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<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="146" xmlns="https://uniprot.org/uniprot">
    <accession>O33777</accession>
    <name>HIS5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Imidazole glycerol phosphate synthase subunit HisH</fullName>
            <ecNumber>4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>IGP synthase glutaminase subunit</fullName>
            <ecNumber>3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName>IGP synthase subunit HisH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>ImGP synthase subunit HisH</fullName>
            <shortName>IGPS subunit HisH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hisH</name>
        <name type="ordered locus">SSO0600</name>
        <name type="ORF">C08_050</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="J. Bacteriol." volume="179" first="4429" last="4432">
            <title>Evolutionary analysis of the hisCGABdFDEHI gene cluster from the archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Sensen C.W."/>
                <person name="Doolittle W.F."/>
                <person name="Brown J.R."/>
            </authorList>
            <dbReference type="PubMed" id="9209067"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.3.2.10"/>
    <dbReference type="EC" id="3.5.1.2"/>
    <dbReference type="EMBL" id="U82227">
        <property type="protein sequence ID" value="AAB63025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90206">
        <property type="entry name" value="H90206"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991119.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O33777"/>
    <dbReference type="SMR" id="O33777"/>
    <dbReference type="FunCoup" id="O33777">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0600"/>
    <dbReference type="MEROPS" id="C26.965"/>
    <dbReference type="PaxDb" id="273057-SSO0600"/>
    <dbReference type="EnsemblBacteria" id="AAK40911">
        <property type="protein sequence ID" value="AAK40911"/>
        <property type="gene ID" value="SSO0600"/>
    </dbReference>
    <dbReference type="GeneID" id="1454878"/>
    <dbReference type="GeneID" id="44129601"/>
    <dbReference type="KEGG" id="sso:SSO0600"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.607"/>
    <dbReference type="eggNOG" id="arCOG00089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071837_2_2_2"/>
    <dbReference type="InParanoid" id="O33777"/>
    <dbReference type="PhylomeDB" id="O33777"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009382">
        <property type="term" value="C:imidazoleglycerol-phosphate synthase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701:SF1">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000495">
        <property type="entry name" value="Amidotransf_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152469" description="Imidazole glycerol phosphate synthase subunit HisH">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1">
        <location>
            <begin position="2"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <sequence length="199" mass="22533" checksum="17DB1A86724CE095" modified="1998-01-01" version="1">MKALVINYGVGNLYSISSALKRVGFEVTIDNKPRNDYDLIVFPGVGAFSAVAEFILRYRELFNDLRRSGTNFLGVCLGMQIMFEKGTEGKESNGLGWFKGIVDKINANVKLPHIGWDLVFEVKDSCELTYGLDKKYVYYVHSYVAYPTSGDYVYMKSQYGIEYPALVCDKNVVGTQFHPEKSSNTGKIFLENLKGWIKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1995-02-01" modified="2026-01-28" version="174" xmlns="https://uniprot.org/uniprot">
    <accession>P39460</accession>
    <name>G3P_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <shortName>GAPDH</shortName>
            <ecNumber>1.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">gap</name>
        <name type="synonym">agaPD</name>
        <name type="ordered locus">SSO0528</name>
        <name type="ORF">C22_023</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1993" name="Biochem. Genet." volume="31" first="241" last="251">
            <title>Nucleotide sequence and molecular evolution of the gene coding for glyceraldehyde-3-phosphate dehydrogenase in the thermoacidophilic archaebacterium Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Arcari P."/>
                <person name="Russo A.D."/>
                <person name="Ianniciello G."/>
                <person name="Gallo M."/>
                <person name="Bocchini V."/>
            </authorList>
            <dbReference type="PubMed" id="8259927"/>
            <dbReference type="DOI" id="10.1007/BF00557333"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PARTIAL PROTEIN SEQUENCE</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1995" name="Eur. J. Biochem." volume="233" first="800" last="808">
            <title>The phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from the thermophilic archaeon Sulfolobus solfataricus overlap by 8-bp. Isolation, sequencing of the genes and expression in Escherichia coli.</title>
            <authorList>
                <person name="Jones C.E."/>
                <person name="Fleming T.M."/>
                <person name="Cowan D.A."/>
                <person name="Littlechild J.A."/>
                <person name="Piper P.W."/>
            </authorList>
            <dbReference type="PubMed" id="8521845"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1995.800_3.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="1999" name="J. Mol. Biol." volume="291" first="651" last="660">
            <title>Crystal structure of the glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Isupov M.N."/>
                <person name="Fleming T.M."/>
                <person name="Dalby A.R."/>
                <person name="Crowhurst G.S."/>
                <person name="Bourne P.C."/>
                <person name="Littlechild J.A."/>
            </authorList>
            <dbReference type="PubMed" id="10448043"/>
            <dbReference type="DOI" id="10.1006/jmbi.1999.3003"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text>Can use both NAD and NADP as cofactors, but exhibits a marked preference for NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>D-glyceraldehyde 3-phosphate + phosphate + NADP(+) = (2R)-3-phospho-glyceroyl phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10296"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)</text>
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    </comment>
    <comment type="pathway">
        <text>Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
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    <comment type="subunit">
        <text>Homotetramer.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="340"/>
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    <feature type="active site" description="Nucleophile" evidence="1">
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            <position position="139"/>
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            <begin position="11"/>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="300"/>
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        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
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    <feature type="disulfide bond">
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            <begin position="123"/>
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    <feature type="sequence conflict" description="In Ref. 1; CAA47040." evidence="2" ref="1">
        <original>I</original>
        <variation>Y</variation>
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            <position position="48"/>
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    <feature type="sequence conflict" description="In Ref. 1; CAA47040." evidence="2" ref="1">
        <original>V</original>
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            <position position="52"/>
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    <feature type="sequence conflict" description="In Ref. 1; CAA47040." evidence="2" ref="1">
        <original>ED</original>
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            <begin position="72"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA47040." evidence="2" ref="1">
        <original>K</original>
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    </feature>
    <feature type="helix" evidence="3">
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="337"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1B7G"/>
        </source>
    </evidence>
    <sequence length="340" mass="37596" checksum="609C9260C3062B6D" modified="2000-12-01" version="2">MINVAVNGYGTIGKRVADAIIKQPDMKLVGVAKTSPNYEAFIAHRRGIRIYVPQQSIKKFEESGIPVAGTVEDLIKTSDIVVDTTPNGVGAQYKPIYLQLQRNAIFQGGEKAEVADISFSALCNYNEALGKKYIRVVSCNTTALLRTICTVNKVSKVEKVRATIVRRAADQKEVKKGPINSLVPDPATVPSHHAKDVNSVIRNLDIATMAVIAPTTLMHMHFINITLKDKVEKKDILSVLENTPRIVLISSKYDAEATAELVEVARDLKRDRNDIPEVMIFSDSIYVKDDEVMLMYAVHQESIVVPENIDAIRASMKLMSAEDSMRITNESLGILKGYLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2026-01-28" version="149" xmlns="https://uniprot.org/uniprot">
    <accession>P50383</accession>
    <name>TRPB1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Tryptophan synthase beta chain 1</fullName>
            <ecNumber>4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">trpB1</name>
        <name type="synonym">trpB</name>
        <name type="ordered locus">SSO0888</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="1995-07" db="EMBL/GenBank/DDBJ databases">
            <title>The tryptophan operon in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Tutino M.L."/>
                <person name="Cubellis M."/>
                <person name="Sannia G."/>
                <person name="Marino G."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
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<entry dataset="Swiss-Prot" created="1992-12-01" modified="2026-01-28" version="156" xmlns="https://uniprot.org/uniprot">
    <accession>P80148</accession>
    <accession>P77979</accession>
    <name>CISY_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Citrate synthase</fullName>
            <ecNumber>2.3.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">gltA</name>
        <name type="ordered locus">SSO2589</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="1996-10" db="EMBL/GenBank/DDBJ databases">
            <title>Sulfolobus solfataricus citrate synthase.</title>
            <authorList>
                <person name="Connaris H."/>
                <person name="Danson M.J."/>
                <person name="Hough D.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35091 / DSM 1616 / IFO 15331 / JCM 8930 / P1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1992" name="Eur. J. Biochem." volume="208" first="459" last="466">
            <title>Conversion, by limited proteolysis, of an archaebacterial citrate synthase into essentially a citryl-CoA hydrolase.</title>
            <authorList>
                <person name="Lill U."/>
                <person name="Lefrank S."/>
                <person name="Henschen A."/>
                <person name="Eggerer H."/>
            </authorList>
            <dbReference type="PubMed" id="1521537"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1992.tb17208.x"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 7-30</scope>
        <scope>ACETYLATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
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        </reaction>
    </comment>
    <comment type="activity regulation">
        <text>Allosterically inhibited by NADH.</text>
    </comment>
    <comment type="pathway">
        <text>Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text>Homodimer.</text>
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    <comment type="PTM">
        <text evidence="2">The N-terminus is blocked by acetylation.</text>
    </comment>
    <comment type="miscellaneous">
        <text>Citrate synthase is found in nearly all cells capable of oxidative metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the citrate synthase family.</text>
    </comment>
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    <feature type="sequence conflict" description="In Ref. 3; AA sequence." evidence="3" ref="3">
        <original>F</original>
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            <position position="20"/>
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        <original>I</original>
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            <position position="27"/>
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    <feature type="sequence conflict" description="In Ref. 1; AAB09594." evidence="3" ref="1">
        <original>P</original>
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            <position position="57"/>
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            <dbReference type="PDB" id="1O7X"/>
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    <sequence length="377" mass="42752" checksum="E3036E232F134F11" modified="2001-06-01" version="3">MSVVSKGLENVIIKVTNLTFIDGEKGILRYRGYNIEDLVNYGSYEETIYLMLYGKLPTKKELNDLKAKLNEEYEVPQEVLDTIYLMPKEADAIGLLEVGTAALASIDKNFKWKENDKEKAISIIAKMATLVANVYRRKEGNKPRIPEPSDSFAKSFLLASFAREPTTDEINAMDKALILYTDHEVPASTTAALVAASTLSDMYSSLTAALAALKGPLHGGAAEEAFKQFIEIGDPNRVQNWFNDKVVNQKNRLMGFGHRVYKTYDPRAKIFKKLALTLIERNADARRYFEIAQKLEELGIKQFSSKGIYPNTDFYSGIVFYALGFPVYMFTALFALSRTLGWLAHIIEYVEEQHRLIRPRALYVGPEYQEYVSIDKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2025-06-18" version="139" xmlns="https://uniprot.org/uniprot">
    <accession>P95968</accession>
    <name>SYE_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="1">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gltX</name>
        <name type="ordered locus">SSO0093</name>
        <name type="ORF">C04029</name>
        <name type="ORF">C05_017</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
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        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000119731" description="Glutamate--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02076"/>
        </source>
    </evidence>
    <sequence length="575" mass="66805" checksum="150D562D4BFED19E" modified="1997-05-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="142" xmlns="https://uniprot.org/uniprot">
    <accession>P95999</accession>
    <name>GGPS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Geranylgeranyl diphosphate synthase</fullName>
            <shortName>GGPP synthase</shortName>
            <shortName>GGPS</shortName>
            <ecNumber>2.5.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">gds</name>
        <name type="synonym">gds-1</name>
        <name type="ordered locus">SSO0061</name>
        <name type="ORF">C05010</name>
        <name type="ORF">C05_049</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of isopentenyl pyrophosphate with the allylic pyrophosphates to yield geranylgeranyl diphosphate (GGPP) which is a precursor of the ether-linked lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = (2E,6E,10E)-geranylgeranyl diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17653"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Isoprenoid biosynthesis; geranylgeranyl diphosphate biosynthesis; geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.29"/>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90145">
        <property type="entry name" value="H90145"/>
    </dbReference>
    <dbReference type="PIR" id="S75427">
        <property type="entry name" value="S75427"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988863.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95999"/>
    <dbReference type="SMR" id="P95999"/>
    <dbReference type="FunCoup" id="P95999">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0061"/>
    <dbReference type="PaxDb" id="273057-SSO0061"/>
    <dbReference type="EnsemblBacteria" id="AAK40423">
        <property type="protein sequence ID" value="AAK40423"/>
        <property type="gene ID" value="SSO0061"/>
    </dbReference>
    <dbReference type="GeneID" id="1455314"/>
    <dbReference type="GeneID" id="44129024"/>
    <dbReference type="KEGG" id="sso:SSO0061"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.62"/>
    <dbReference type="eggNOG" id="arCOG01726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_2_1_2"/>
    <dbReference type="InParanoid" id="P95999"/>
    <dbReference type="PhylomeDB" id="P95999"/>
    <dbReference type="UniPathway" id="UPA00389">
        <property type="reaction ID" value="UER00564"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033386">
        <property type="term" value="P:geranylgeranyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053504">
        <property type="entry name" value="GGPP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041003">
        <property type="entry name" value="GGPP_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF85">
        <property type="entry name" value="SHORT CHAIN ISOPRENYL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01017">
        <property type="entry name" value="Polyprenyl_Transferase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000123974" description="Geranylgeranyl diphosphate synthase">
        <location>
            <begin position="1"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>isopentenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>isopentenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>isopentenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>an all-trans-polyprenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>isopentenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>an all-trans-polyprenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>an all-trans-polyprenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>an all-trans-polyprenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>an all-trans-polyprenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>an all-trans-polyprenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
        </ligand>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA69541." evidence="4" ref="1">
        <original>A</original>
        <variation>S</variation>
        <location>
            <position position="122"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA69541." evidence="4" ref="1">
        <original>A</original>
        <variation>S</variation>
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P14324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q12051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="332" mass="37228" checksum="2E3BEC59432CBD41" modified="2001-06-20" version="2">MSSDDLSLYFNEIVNNVNFRIKNFVKSNFKTLEEASFHLFTAGGKRLRPLVLVSSSDLIGGDRERAYKAAAAVEILHNFTLVHDDIMDNDGLRRGLPTVHVKWGEPMAILAGDYLHAKAFEALNEALKGLDGNTFYKAFSIFITSIEIISEGQAMDMSFENRLDVTEEEYIQMIKGKTAMLFSCSAALGGIINKANDDVVKKLTEYGLNLGISFQIVDDILGIIGDEKELGKPIYSDIREGKKTILVIKTLSEATEDEKKILVSTLGNKEAKKEDLERASEIIRKHSLQYAYDLAKKYSDLAIENLREIPVSNKTAEKALKYLAQFTIQRRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>P96037</accession>
    <name>RL11_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl11</name>
        <name evidence="1" type="synonym">rpl11Ab</name>
        <name type="ordered locus">SSO0346</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="Biochim. Biophys. Acta" volume="1340" first="170" last="177">
            <title>Nucleotide sequence of a gene cluster encoding NusG and the L11-L1-L10-L12 ribosomal proteins from the thermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Geiger M."/>
                <person name="Groebner P."/>
                <person name="Piendl W."/>
            </authorList>
            <dbReference type="PubMed" id="9252104"/>
            <dbReference type="DOI" id="10.1016/S0167-4838(97)00073-3"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40675" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="U85262">
        <property type="protein sequence ID" value="AAB99524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40675.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="PIR" id="D90177">
        <property type="entry name" value="D90177"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990636.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P96037"/>
    <dbReference type="SMR" id="P96037"/>
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        <property type="interactions" value="211"/>
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    <dbReference type="STRING" id="273057.SSO0346"/>
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    <dbReference type="EnsemblBacteria" id="AAK40675">
        <property type="protein sequence ID" value="AAK40675"/>
        <property type="gene ID" value="SSO0346"/>
    </dbReference>
    <dbReference type="GeneID" id="1455485"/>
    <dbReference type="KEGG" id="sso:SSO0346"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.336"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074237_4_0_2"/>
    <dbReference type="InParanoid" id="P96037"/>
    <dbReference type="PhylomeDB" id="P96037"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
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    </dbReference>
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        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="entry name" value="PRK01143.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000104448" description="Large ribosomal subunit protein uL11">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="170" mass="18182" checksum="967A2CD154032F1D" modified="1997-05-01" version="1">MPIKTIKIMVEGGNVKPGPPLAPTLSQLGLNVGEVVKKLNEATSSFKGMSVPVTIEVDSSTKKYEIKVGIPTTTALLLKEAGASEPSGDPAHKKIGNLSLEQVIKIAIMKKPGLTTKSLKAALKSMLGTAKSIGLTVDNRDPKELVKEVEEGKYDDLLAKYENEWNGVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-03-27" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX55</accession>
    <name>Q7LX55_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2203 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2148</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74061">
        <property type="entry name" value="S74061"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX55"/>
    <dbReference type="STRING" id="273057.SSO2148"/>
    <dbReference type="PaxDb" id="273057-SSO2148"/>
    <dbReference type="EnsemblBacteria" id="AAK42325">
        <property type="protein sequence ID" value="AAK42325"/>
        <property type="gene ID" value="SSO2148"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2148"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2242"/>
    <dbReference type="eggNOG" id="arCOG05495">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137908_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LX55"/>
    <dbReference type="PhylomeDB" id="Q7LX55"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018699">
        <property type="entry name" value="DUF2203"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09969">
        <property type="entry name" value="DUF2203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016498">
        <property type="entry name" value="UCP016498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="15199" checksum="19DFC3B115C1961E" modified="2011-05-31" version="1">MRILIFVEYPYFDLETARELLPWLRDRLIQLRKIKNEIELLLVNGDKYALQQYASETKKIIDEIISKGIILRDIDIGLVDFPAIINNKPAFFCWKIDENDIAYWHYMDEGFRGRKRLTGYEDILSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXA5</accession>
    <name>Q7LXA5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptidase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2088</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73083">
        <property type="entry name" value="S73083"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989795.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXA5"/>
    <dbReference type="FunCoup" id="Q7LXA5">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2088"/>
    <dbReference type="PaxDb" id="273057-SSO2088"/>
    <dbReference type="EnsemblBacteria" id="AAK42267">
        <property type="protein sequence ID" value="AAK42267"/>
        <property type="gene ID" value="SSO2088"/>
    </dbReference>
    <dbReference type="GeneID" id="1453594"/>
    <dbReference type="KEGG" id="sso:SSO2088"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2166"/>
    <dbReference type="eggNOG" id="arCOG03669">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_451752_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXA5"/>
    <dbReference type="PhylomeDB" id="Q7LXA5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000062">
        <property type="entry name" value="Peptidase S53 propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF15">
        <property type="entry name" value="TRIPEPTIDYL-PEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S53" evidence="8">
        <location>
            <begin position="230"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="606" mass="67086" checksum="79DF9847AA5EA660" modified="2011-05-31" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXE8</accession>
    <name>Q7LXE8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">dTDP-Glucose 4,6-dehydratase (RfbB-1)</fullName>
            <ecNumber evidence="3">4.2.1.46</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rfbB-1</name>
        <name evidence="3" type="ordered locus">SSO0830</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.46" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90233">
        <property type="entry name" value="F90233"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923120.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXE8"/>
    <dbReference type="FunCoup" id="Q7LXE8">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0830"/>
    <dbReference type="PaxDb" id="273057-SSO0830"/>
    <dbReference type="EnsemblBacteria" id="AAK41125">
        <property type="protein sequence ID" value="AAK41125"/>
        <property type="gene ID" value="SSO0830"/>
    </dbReference>
    <dbReference type="GeneID" id="1455092"/>
    <dbReference type="KEGG" id="sso:SSO0830"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.845"/>
    <dbReference type="eggNOG" id="arCOG01371">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_2"/>
    <dbReference type="InParanoid" id="Q7LXE8"/>
    <dbReference type="PhylomeDB" id="Q7LXE8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="317" mass="36487" checksum="4ED45BD31F8AE22E" modified="2011-05-31" version="1">MKIIVVGGAGFIGSAFVRELNKRGIKPIVVDLLTYAGRKENLIGTDYDFVQADVRSEKMHDIIKEYSPDIVINFAAETHVDRSIYKPQDFVTTNVLGTVNLLEASRKFNFKYVHISTDEVYGEECGDENSPLKPSSPYSASKASADLFVKAYVRTYGISAVIVRPSNNYGPRQFPEKLIPKAIIRTLLGIHIPVYGDGKAERDWIFVEDTARIIFDVVSRAEWKGEVYNIPGGQRYNVLEILKMLEEVSGKEVKIKFVSDRPGHDRRYCMTTSMKYEVTPFKEGLRRTYEWYLNNRWWWEPLINDKFFVEDEPWLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXU8</accession>
    <name>Q7LXU8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosomal protein L11 methyltransferase, putative</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO0554</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90201">
        <property type="entry name" value="H90201"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991062.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXU8"/>
    <dbReference type="FunCoup" id="Q7LXU8">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0554"/>
    <dbReference type="PaxDb" id="273057-SSO0554"/>
    <dbReference type="EnsemblBacteria" id="AAK40871">
        <property type="protein sequence ID" value="AAK40871"/>
        <property type="gene ID" value="SSO0554"/>
    </dbReference>
    <dbReference type="GeneID" id="1454838"/>
    <dbReference type="KEGG" id="sso:SSO0554"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.564"/>
    <dbReference type="eggNOG" id="arCOG01631">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068443_2_1_2"/>
    <dbReference type="InParanoid" id="Q7LXU8"/>
    <dbReference type="PhylomeDB" id="Q7LXU8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026170">
        <property type="entry name" value="FAM173A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053600">
        <property type="entry name" value="Lysine_N-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041081">
        <property type="entry name" value="prot_lys_mtase_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13610">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13610:SF11">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="4">
        <location>
            <begin position="29"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="161" mass="18154" checksum="626265D801B14834" modified="2011-05-31" version="1">MSYVPHVPYVPTPEKVVRRMLEIAKASQDDIVYDLGCGDGRIIITAVKDFNVKKAIGVEINDERIREALANIEKNGVTGRASIVKGNFFEVDISEATVVTMFLLTNVNEMLKPKLEKELKPGTRVVSHEFEIRGWNPKEVVKVEDGNMNHTVYLYVIGEHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXY7</accession>
    <name>Q7LXY7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75382">
        <property type="entry name" value="S75382"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988926.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXY7"/>
    <dbReference type="SMR" id="Q7LXY7"/>
    <dbReference type="PaxDb" id="273057-SSO0107"/>
    <dbReference type="EnsemblBacteria" id="AAK40462">
        <property type="protein sequence ID" value="AAK40462"/>
        <property type="gene ID" value="SSO0107"/>
    </dbReference>
    <dbReference type="GeneID" id="1453290"/>
    <dbReference type="KEGG" id="sso:SSO0107"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.103"/>
    <dbReference type="eggNOG" id="arCOG05989">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166553_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXY7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="25"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="123" mass="14021" checksum="FD2F68340086E91A" modified="2011-05-31" version="1">MEKAIHNLGKDARLHIIHILLQNRSKKELADELGITPAAITKYLKGITHPSDEIIEKCIEIASEDEEYHEIIKVIVSDITEALMELLREIGIEKMIDNEDIQKLKKLLDKAFDKMLSTSPSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-11-27" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TY8</accession>
    <name>Q97TY8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ornithine cyclodeaminase related protein (Ocd-like2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ocd-like2</name>
        <name evidence="1" type="ordered locus">SSO3243</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99510">
        <property type="entry name" value="A99510"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924174.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TY8"/>
    <dbReference type="STRING" id="273057.SSO3243"/>
    <dbReference type="PaxDb" id="273057-SSO3243"/>
    <dbReference type="EnsemblBacteria" id="AAK43336">
        <property type="protein sequence ID" value="AAK43336"/>
        <property type="gene ID" value="SSO3243"/>
    </dbReference>
    <dbReference type="GeneID" id="1453255"/>
    <dbReference type="KEGG" id="sso:SSO3243"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3343"/>
    <dbReference type="eggNOG" id="arCOG01035">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042088_2_1_2"/>
    <dbReference type="InParanoid" id="Q97TY8"/>
    <dbReference type="PhylomeDB" id="Q97TY8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001456">
        <property type="entry name" value="Ornithine cyclodeaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="entry name" value="ornithine cyclodeaminase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812:SF19">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="318" mass="35857" checksum="66A781D8D4B8F983" modified="2001-10-01" version="1">MTLLLKENDVINLLDYKQIYGALVNAFLLFENKFAINLERSRISFHGTTLTFQAAGMENYIGYKTFISGNHLTFLYDTSGNLLSIIESDRLSQARTAVLSILATDYVYGDFSSLGVIGLGKYGLAIVEIANQLKKGIKINVFTPSQQRMEKALSILRGEGIDVSPKDSIKRVCEESEVVTTITKAKDPFLKLEYVNNKRIHINAMGSNIPEKIEIFPEVIKASNLIVVEELEHEFKRIRRVSHRKRMGMLDMSKITLLSWILSRKINVQKEGITIFKSVGIGLEDLAVGKLIYEKALSKGLGTEIEVKGVWYRESEKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2013-06-26" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U29</accession>
    <name>KDGK_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>2-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinase</fullName>
            <shortName>2-dehydro-3-deoxyglucono/galactono-kinase</shortName>
            <ecNumber evidence="1 2 3">2.7.1.178</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>2-keto-3-deoxy-galactonokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>2-keto-3-deoxygluconokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>3-deoxy-2-oxo-D-gluconate kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>KDG kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>KDGal kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">kdgK</name>
        <name type="ordered locus">SSO3195</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Biochem. J." volume="390" first="529" last="540">
            <title>The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluation.</title>
            <authorList>
                <person name="Ahmed H."/>
                <person name="Ettema T.J."/>
                <person name="Tjaden B."/>
                <person name="Geerling A.C."/>
                <person name="van der Oost J."/>
                <person name="Siebers B."/>
            </authorList>
            <dbReference type="PubMed" id="15869466"/>
            <dbReference type="DOI" id="10.1042/BJ20041711"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2005" name="FEBS Lett." volume="579" first="6865" last="6869">
            <title>Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Lamble H.J."/>
                <person name="Theodossis A."/>
                <person name="Milburn C.C."/>
                <person name="Taylor G.L."/>
                <person name="Bull S.D."/>
                <person name="Hough D.W."/>
                <person name="Danson M.J."/>
            </authorList>
            <dbReference type="PubMed" id="16330030"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2005.11.028"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>MASS SPECTROMETRY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2006" name="Biosci. Biotechnol. Biochem." volume="70" first="1308" last="1316">
            <title>Characterization of Sulfolobus solfataricus 2-keto-3-deoxy-D-gluconate kinase in the modified Entner-Doudoroff pathway.</title>
            <authorList>
                <person name="Kim S."/>
                <person name="Lee S.B."/>
            </authorList>
            <dbReference type="PubMed" id="16794308"/>
            <dbReference type="DOI" id="10.1271/bbb.50566"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="5">
        <citation type="journal article" date="2008" name="Acta Crystallogr. D" volume="64" first="1283" last="1287">
            <title>The structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase.</title>
            <authorList>
                <person name="Potter J.A."/>
                <person name="Kerou M."/>
                <person name="Lamble H.J."/>
                <person name="Bull S.D."/>
                <person name="Hough D.W."/>
                <person name="Danson M.J."/>
                <person name="Taylor G.L."/>
            </authorList>
            <dbReference type="PubMed" id="19018105"/>
            <dbReference type="DOI" id="10.1107/S0907444908036111"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH ATP ANALOGS AND 2-KETO-3-DEOXYGLUCONATE</scope>
        <scope>ACTIVE SITE</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 3">Involved in the degradation of glucose and galactose via the semi-phosphorylative Entner-Doudoroff pathway. Catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) and 2-keto-3-deoxygalactonate (KDGal) to produce 2-keto-3-deoxy-6-phosphogluconate (KDPG) and 2-keto-3-deoxy-6-phosphogalactonate (KDPGal), respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2 3">
            <text>2-dehydro-3-deoxy-D-gluconate + ATP = 2-dehydro-3-deoxy-6-phospho-D-gluconate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14797"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57569"/>
            <dbReference type="ChEBI" id="CHEBI:57990"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.178"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2 3">
            <text>2-dehydro-3-deoxy-D-galactonate + ATP = 2-dehydro-3-deoxy-6-phospho-D-galactonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16525"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57989"/>
            <dbReference type="ChEBI" id="CHEBI:58298"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.178"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2 3">0.14 mM for KDG (at 70 degrees Celsius and pH 7.2)</KM>
            <KM evidence="2 3">2.8 mM for ATP (at 60 degrees Celsius and pH 7.5)</KM>
            <KM evidence="2 3">3.6 mM for KDG (at 60 degrees Celsius and pH 7.5)</KM>
            <KM evidence="2 3">8.1 mM for KDGal (at 60 degrees Celsius and pH 7.5)</KM>
            <Vmax evidence="2 3">9.7 umol/min/mg enzyme</Vmax>
            <text>kcat is 3.8 sec(-1) for ATP, 5 sec(-1) for KDG and 5.4 sec(-1) for KDGal (at 60 degrees Celsius and pH 7.5).</text>
        </kinetics>
        <phDependence>
            <text evidence="2 3">Optimum pH is between 7 and 8.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2 3">Optimum temperature is between 70 and 80 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Carbohydrate acid metabolism; 2-dehydro-3-deoxy-D-gluconate degradation; D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Homohexamer; trimer of dimers.</text>
    </comment>
    <comment type="mass spectrometry" mass="34870.0" method="MALDI" evidence="2"/>
    <comment type="similarity">
        <text evidence="5">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.178" evidence="1 2 3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90504">
        <property type="entry name" value="F90504"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991690.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2V78">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B/C=1-313"/>
    </dbReference>
    <dbReference type="PDB" id="2VAR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B/C=1-313"/>
    </dbReference>
    <dbReference type="PDBsum" id="2V78"/>
    <dbReference type="PDBsum" id="2VAR"/>
    <dbReference type="AlphaFoldDB" id="Q97U29"/>
    <dbReference type="SMR" id="Q97U29"/>
    <dbReference type="FunCoup" id="Q97U29">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3195"/>
    <dbReference type="PaxDb" id="273057-SSO3195"/>
    <dbReference type="EnsemblBacteria" id="AAK43293">
        <property type="protein sequence ID" value="AAK43293"/>
        <property type="gene ID" value="SSO3195"/>
    </dbReference>
    <dbReference type="GeneID" id="1453212"/>
    <dbReference type="GeneID" id="44128912"/>
    <dbReference type="KEGG" id="sso:SSO3195"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3299"/>
    <dbReference type="eggNOG" id="arCOG00014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_6_0_2"/>
    <dbReference type="InParanoid" id="Q97U29"/>
    <dbReference type="PhylomeDB" id="Q97U29"/>
    <dbReference type="BioCyc" id="MetaCyc:MONOMER-17931"/>
    <dbReference type="BRENDA" id="2.7.1.178">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00856">
        <property type="reaction ID" value="UER00828"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97U29"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008671">
        <property type="term" value="F:2-dehydro-3-deoxygalactonokinase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008673">
        <property type="term" value="F:2-dehydro-3-deoxygluconokinase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd01166">
        <property type="entry name" value="KdgK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000088">
        <property type="entry name" value="2-dehydro-3-deoxygluconokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054939">
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            <dbReference type="PubMed" id="16794308"/>
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    </evidence>
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    </evidence>
    <sequence length="313" mass="34875" checksum="43D5787DB4D3A86F" modified="2001-10-01" version="1">MVDVIALGEPLIQFNSFNPGPLRFVNYFEKHVAGSELNFCIAVVRNHLSCSLIARVGNDEFGKNIIEYSRAQGIDTSHIKVDNESFTGIYFIQRGYPIPMKSELVYYRKGSAGSRLSPEDINENYVRNSRLVHSTGITLAISDNAKEAVIKAFELAKSRSLDTNIRPKLWSSLEKAKETILSILKKYDIEVLITDPDDTKILLDVTDPDEAYRKYKELGVKVLLYKLGSKGAIAYKDNVKAFKDAYKVPVEDPTGAGDAMAGTFVSLYLQGKDIEYSLAHGIAASTLVITVRGDNELTPTLEDAERFLNEFKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U65</accession>
    <name>Q97U65_SACS2</name>
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        <submittedName>
            <fullName evidence="3">Lipoate-protein ligase A (LplA-1)</fullName>
        </submittedName>
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        <name evidence="3" type="primary">lplA-1</name>
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        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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                <person name="Van der Oost J."/>
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            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2.</text>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
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            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="247" mass="28410" checksum="DF341197ADF11B92" modified="2001-10-01" version="1">MKLRVLLTEYPNDPYLNVAMDEALLLHSKYVPILRIWRNEKSVVLGILSSISDEVNLEMIKKYDVKLARRISGGGSVFHDMGNINYTFITSGQGGIDYLYGYLLRGTISAIENLVNDRVEVYNETDIAFKGYKISGNAGYINEDKYLLHGTLLVSSNLDILHKVLIIPPKNYRKKDINMIKYRVSNLSNLVKGDITYDEAIEAFIRGFSSLLRCDDYFFDDISDEELKLAMRLANEKYSKKEFIYKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U89</accession>
    <name>Q97U89_SACS2</name>
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        <submittedName>
            <fullName evidence="2">Heterodisulfide reductase related protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">SSO3131</name>
    </gene>
    <organism evidence="2 3">
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        <dbReference type="NCBI Taxonomy" id="273057"/>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43232.1"/>
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    </dbReference>
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        <property type="entry name" value="A99497"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="AAK43232">
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        <property type="gene ID" value="SSO3131"/>
    </dbReference>
    <dbReference type="GeneID" id="1453151"/>
    <dbReference type="KEGG" id="sso:SSO3131"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3237"/>
    <dbReference type="eggNOG" id="arCOG00332">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023081_2_1_2"/>
    <dbReference type="InParanoid" id="Q97U89"/>
    <dbReference type="PhylomeDB" id="Q97U89"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051460">
        <property type="entry name" value="HdrC_iron-sulfur_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255:SF2">
        <property type="entry name" value="HETERODISULFIDE REDUCTASE RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cysteine-rich" evidence="1">
        <location>
            <begin position="199"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="299" mass="34319" checksum="04344BB2B7A7A011" modified="2001-10-01" version="1">MINEDQLNFIRKNLVKYLMEDYLPFPVNRSVCYEWANGLNIRRGGETIIYTGCSYQLAELGKRFDEILPALSKFKGVERFSSILKVFYKPKDTRSYKILRNIASVLKSSVDFGYLYEDEPYSGTILLEMGMVEEFKEYAKKLVEVFDSHGVKRIITVDPHTHYTLFRIKEMLSPSWNVEIVNYFELIKNVKVKGEGTFVFHDSCLYSRFLGMRDSIREVIKSSGIVLKEDEMITGKETSMCCGGPLAPINKETSDKIARNRAEALKSVHNKVLLACPFCYANLSPYVEAYDFAEVISGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UA5</accession>
    <name>Q97UA5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acetyl-CoA c-acetyltransferase (Acetoacetyl-CoA thiolase) (AcaB-10)</fullName>
            <ecNumber evidence="3">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">acaB-10</name>
        <name evidence="3" type="ordered locus">SSO3113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.9" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99495">
        <property type="entry name" value="A99495"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990937.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UA5"/>
    <dbReference type="STRING" id="273057.SSO3113"/>
    <dbReference type="PaxDb" id="273057-SSO3113"/>
    <dbReference type="DNASU" id="1453135"/>
    <dbReference type="EnsemblBacteria" id="AAK43216">
        <property type="protein sequence ID" value="AAK43216"/>
        <property type="gene ID" value="SSO3113"/>
    </dbReference>
    <dbReference type="GeneID" id="15298635"/>
    <dbReference type="KEGG" id="sso:SSO3113"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3220"/>
    <dbReference type="eggNOG" id="arCOG01281">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_2_1_2"/>
    <dbReference type="InParanoid" id="Q97UA5"/>
    <dbReference type="PhylomeDB" id="Q97UA5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF2">
        <property type="entry name" value="LIPID-TRANSFER PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase C-terminal" evidence="2">
        <location>
            <begin position="269"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="385" mass="42341" checksum="7F5C4067D7A86ACC" modified="2001-10-01" version="1">MITGFASSLYKKYEGSTFELLADTVFSALEMAGLDKNYVDGIYLTYLPGTFDGYANSHFFTNQVAQYLGIRPKFTQIFDYGGASALSMIHRAYKAIKAGEGETILCIVGGKATELRNKGVTVDAIDKAYPNVSLTPFDRLFRGLNDLNPVSDYALVANRHKYLFKSTDEQRALIAVRQRFNAMQNSKAMFKDPLTIEQVLKSPLVSYPLHLLEIVYPIDGFHVFVVSKRSSKSALTNIDILGYGEAHWPFMPTELDEIVYTPAVESSKGLLNGRIDAFELYDSFTITVMLQVEDIGLAEKGKGGSFFENHDTTFKGDVPINTGGGSLNTGQPAYMSGGVILEEALLQLNDMADGHQVKGTDVVFLNGIGGWNRSHSVSLVLGEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="144" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UF2</accession>
    <name>Q97UF2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Molybdate/tungstate import ATP-binding protein WtpC</fullName>
            <ecNumber evidence="10">7.3.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">SSO3069</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Part of the ABC transporter complex WtpABC involved in molybdate/tungstate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tungstate(in) + ATP + H2O = tungstate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35027"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">The complex is composed of two ATP-binding proteins (WtpC), two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.6" evidence="10"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90489">
        <property type="entry name" value="B90489"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990873.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UF2"/>
    <dbReference type="FunCoup" id="Q97UF2">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3069"/>
    <dbReference type="TCDB" id="3.A.1.1.14">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3069"/>
    <dbReference type="EnsemblBacteria" id="AAK43169">
        <property type="protein sequence ID" value="AAK43169"/>
        <property type="gene ID" value="SSO3069"/>
    </dbReference>
    <dbReference type="GeneID" id="1453088"/>
    <dbReference type="KEGG" id="sso:SSO3069"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3169"/>
    <dbReference type="eggNOG" id="arCOG00175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_2"/>
    <dbReference type="InParanoid" id="Q97UF2"/>
    <dbReference type="PhylomeDB" id="Q97UF2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055052">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015408">
        <property type="term" value="F:ABC-type ferric iron transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901238">
        <property type="term" value="F:ABC-type tungstate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03259">
        <property type="entry name" value="ABC_Carb_Solutes_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000425">
        <property type="entry name" value="Probable ABC transporter, ATP-binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053598">
        <property type="entry name" value="ABC-Glucose_import_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015853">
        <property type="entry name" value="ABC_transpr_FbpC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047641">
        <property type="entry name" value="ABC_transpr_MalK/UgpC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040856">
        <property type="entry name" value="GlcV_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040933">
        <property type="entry name" value="ABC_arch_GlcV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875:SF4">
        <property type="entry name" value="GLUCOSE IMPORT ATP-BINDING PROTEIN GLCV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875">
        <property type="entry name" value="MALTODEXTRIN IMPORT ATP-BINDING PROTEIN MSMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17847">
        <property type="entry name" value="GlcV_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="MOP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="4"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAK43169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="371" mass="40868" checksum="B0DEBEBC23A09DC1" modified="2001-10-01" version="1">MTTIRVENLSKIFKKGKTEVKAVDNVSITIDSGMAFGVLGPSGHGKTTFLRLIAGLEEPTSGYIYFDNEAVSSPRRVMMSPEKRGIAMVFQNWALYPNMTVFDNIAFPLKLAKVPKDKIENKVKEVSEELGLSGVLNRYPKELSGGQMQRTAIARALVKDPKVLLLDEPFSNLDAQIRESARALVRKIQRERKLTTLIVSHDPADIFAIANKAGVIVNGKFAQIGTPTEIYEYPATDLIARLTGEINLIQAKIIENNAIIANLKVPLNNMELKGQSNIVIGLRPDDLTLSDTLLDKYIDMGIVKVKLVSYGAGIFKIVVSPITDENIDIIVDAEEPLETGIETHLLAKPNKVKIFDLNGSNLITSKTQIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UF7</accession>
    <name>Q97UF7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90488">
        <property type="entry name" value="E90488"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924120.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UF7"/>
    <dbReference type="STRING" id="273057.SSO3063"/>
    <dbReference type="PaxDb" id="273057-SSO3063"/>
    <dbReference type="EnsemblBacteria" id="AAK43164">
        <property type="protein sequence ID" value="AAK43164"/>
        <property type="gene ID" value="SSO3063"/>
    </dbReference>
    <dbReference type="GeneID" id="1453083"/>
    <dbReference type="KEGG" id="sso:SSO3063"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3164"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066381_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UF7"/>
    <dbReference type="PhylomeDB" id="Q97UF7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="1">
        <location>
            <begin position="234"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="355" mass="40852" checksum="FCC1CD710F86D440" modified="2001-10-01" version="1">MVGSISEAKSSVSGVTDPYCRTEHCEKIHPYRCDKEVGVLGIDVSKDNLITSRGGKYENNLKGYEKILDMKPCTIVLEPTGVYSIKPSQYFKEKGIRVLQVSPNVLSREKDFREKKTDFYDAEKLENMVDKAKEYEYNVLRELVTLYLFLKDMEVKQKNRLKRALFLVSDNDKINKERLERLASGDFAQEVLYQLEYTPIVLEEVKILAKALFETQEKLKEVKEMIESQVPEDHVLLTIPGVGKLAAGIIIGVVGDIRRFPKPESFVAYCGLDPVVERSGKAVVSKGISRRGNKYLRSLFYFLAQMNCSKNPTLLRFYESHRNRLHGRKLYTALARKLAKVVWSVWYNNKPYEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UH9</accession>
    <name>Q97UH9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2139 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3038</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90485">
        <property type="entry name" value="E90485"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924104.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UH9"/>
    <dbReference type="PaxDb" id="273057-SSO3038"/>
    <dbReference type="EnsemblBacteria" id="AAK43140">
        <property type="protein sequence ID" value="AAK43140"/>
        <property type="gene ID" value="SSO3038"/>
    </dbReference>
    <dbReference type="GeneID" id="1453059"/>
    <dbReference type="KEGG" id="sso:SSO3038"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3137"/>
    <dbReference type="eggNOG" id="arCOG04132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_907988_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UH9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016675">
        <property type="entry name" value="UCP016666"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09910">
        <property type="entry name" value="DUF2139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="34662" checksum="1C4AD43C923E589F" modified="2001-10-01" version="1">MYFSRADGHAELGLWKVGVSTRDAEFLVKNRTVYKMEMKDDKIYATIFNPIHTDVSSIMVYNTLNGEHKFVESFDFPLENNKKIHIRRVGGQIVQIQNKLIAFYGGALIHIDPQKDVYRLYPFLDVRNPESERPTYIPGFRAQKVYIMGIPIISANPAEDLRDISSKTTFGLLLRIDPVVPQIITESGAIFGMAHDGEHLYIGASYANHTGVYTYRAGDGGIFAIPVSELFRKPWNPIRIWIHDGSYSPTSGIDGWFGGIPLKGFTEKKLRVYTGKSTKMRIAEYSLFSKVRDDTVSLNTGWNIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UJ0</accession>
    <name>Q97UJ0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">One of two inversely orientated ORFs in ISC1043</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90483">
        <property type="entry name" value="G90483"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UJ0"/>
    <dbReference type="STRING" id="273057.SSO3025"/>
    <dbReference type="PaxDb" id="273057-SSO3025"/>
    <dbReference type="EnsemblBacteria" id="AAK43126">
        <property type="protein sequence ID" value="AAK43126"/>
        <property type="gene ID" value="SSO3025"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3025"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3118"/>
    <dbReference type="eggNOG" id="arCOG09862">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1656966_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UJ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="159" mass="18607" checksum="02612FCC2B7FDC75" modified="2001-10-01" version="1">MYHEHMNKKLTQKDFDNFKESIEDLIKLHKESRLSKIENNKENEEERSARLRRFGRELRLRGGKVRQDQERTREKKQTNPTREGIHTDNQGISRLIQQGNRRTSITTEPNEEGDKPQDSRETILPPHIFVILYNLLSKTVGGLKVVMDGTGYSLVVTKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UK4</accession>
    <name>Q97UK4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mannan endo-1,4-beta-mannosidase</fullName>
            <ecNumber evidence="3">3.2.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO3007</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.</text>
            <dbReference type="EC" id="3.2.1.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.2.1.78" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90481">
        <property type="entry name" value="F90481"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992659.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UK4"/>
    <dbReference type="SMR" id="Q97UK4"/>
    <dbReference type="STRING" id="273057.SSO3007"/>
    <dbReference type="CAZy" id="GH5">
        <property type="family name" value="Glycoside Hydrolase Family 5"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3007"/>
    <dbReference type="EnsemblBacteria" id="AAK43109">
        <property type="protein sequence ID" value="AAK43109"/>
        <property type="gene ID" value="SSO3007"/>
    </dbReference>
    <dbReference type="GeneID" id="1453028"/>
    <dbReference type="KEGG" id="sso:SSO3007"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3098"/>
    <dbReference type="eggNOG" id="arCOG05411">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_429428_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UK4"/>
    <dbReference type="PhylomeDB" id="Q97UK4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03143">
        <property type="entry name" value="A4_beta-galactosidase_middle_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045053">
        <property type="entry name" value="MAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451:SF39">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="8">
        <location>
            <begin position="24"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK43109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="597" mass="69317" checksum="88D4AC79F7E941A9" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UP4</accession>
    <name>Q97UP4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="2">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2960</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99476">
        <property type="entry name" value="A99476"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924061.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UP4"/>
    <dbReference type="FunCoup" id="Q97UP4">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2960"/>
    <dbReference type="PaxDb" id="273057-SSO2960"/>
    <dbReference type="EnsemblBacteria" id="AAK43064">
        <property type="protein sequence ID" value="AAK43064"/>
        <property type="gene ID" value="SSO2960"/>
    </dbReference>
    <dbReference type="GeneID" id="1452983"/>
    <dbReference type="KEGG" id="sso:SSO2960"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3050"/>
    <dbReference type="eggNOG" id="arCOG01067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_815399_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UP4"/>
    <dbReference type="PhylomeDB" id="Q97UP4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF47">
        <property type="entry name" value="EXTERNAL NADH-UBIQUINONE OXIDOREDUCTASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="8">
        <location>
            <begin position="1"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK43064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="343" mass="37693" checksum="15ED9C191B7D070F" modified="2001-10-01" version="1">MRVVIVGGGFAGIAAKSKYPNAILIDRSEYFLMTPKIVHTLADGESALVYRKPDIVAEVVNIKFNEKKIITNKGEISYDKLIISLGYSQDLSKIKGANEHVMKLESFEDAIKMHNEIEKARTLIVIGGGDLGVEVVGSTIELVSKIKRKGKERVILINRGARILPHMPPQISLKAENILAELGVEVILDASVEEIRGKTVITSKGEFSGDHIFYAGGIKGSNFLTNLKLSLKDGKINVNEDLSSVDYKDVYGAGVCASTFYPSNAEISMQSGVHAILNAIEGRDEKFKPMPLGDIVDIDNNFMGVFMGLPVRGNFAKLLKRIALAKVYYTINKVNFYNKNLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UV8</accession>
    <name>Q97UV8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90467">
        <property type="entry name" value="A90467"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UV8"/>
    <dbReference type="STRING" id="273057.SSO2886"/>
    <dbReference type="PaxDb" id="273057-SSO2886"/>
    <dbReference type="DNASU" id="1452911"/>
    <dbReference type="EnsemblBacteria" id="AAK42992">
        <property type="protein sequence ID" value="AAK42992"/>
        <property type="gene ID" value="SSO2886"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2886"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2972"/>
    <dbReference type="eggNOG" id="arCOG07927">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1438170_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UV8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="195" mass="22570" checksum="E758C21926E121EA" modified="2001-10-01" version="1">MTNILILMEREIIKILSVVEFHPLILAFHLGLMLAGVSLLSYSLFLILVKKVDIMLPLVISFMAYLTSLILLILILLLETYRFLIFDGRILVKNSLLTRNEFKFSLRDKNLIIGFYDKASLGKFAIIYGDRLLFKFLTQQKNIRLIKNFVVKSGYRLGGTYKICRVCGSINEENAEYCDFCGSREVSPYDLYWID</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V04</accession>
    <name>Q97V04_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative phage metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2833</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90460">
        <property type="entry name" value="H90460"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990886.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V04"/>
    <dbReference type="STRING" id="273057.SSO2833"/>
    <dbReference type="PaxDb" id="273057-SSO2833"/>
    <dbReference type="EnsemblBacteria" id="AAK42943">
        <property type="protein sequence ID" value="AAK42943"/>
        <property type="gene ID" value="SSO2833"/>
    </dbReference>
    <dbReference type="GeneID" id="1452862"/>
    <dbReference type="KEGG" id="sso:SSO2833"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2918"/>
    <dbReference type="eggNOG" id="arCOG04217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136010_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V04"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043998">
        <property type="entry name" value="Put_Metallopep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012372">
        <property type="entry name" value="UCP014405_Zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18894">
        <property type="entry name" value="PhageMetallopep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF014405">
        <property type="entry name" value="UCP014405"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative phage metallopeptidase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="127" mass="14924" checksum="19E865F700A404B3" modified="2001-10-01" version="1">MIKYVRSQDGEELLRDIVITLGLDYINLDRVKVVYSYGSKTEAIARIWAVPKIVLETFQLEPLYVIELISERFNRLSNDDKIKIMIHEILHIPFKFSGGLRAHGSKVNSREVNKLYKKYIKLKERRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V12</accession>
    <name>Q97V12_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90459">
        <property type="entry name" value="H90459"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989016.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V12"/>
    <dbReference type="STRING" id="273057.SSO2825"/>
    <dbReference type="PaxDb" id="273057-SSO2825"/>
    <dbReference type="EnsemblBacteria" id="AAK42935">
        <property type="protein sequence ID" value="AAK42935"/>
        <property type="gene ID" value="SSO2825"/>
    </dbReference>
    <dbReference type="GeneID" id="1452854"/>
    <dbReference type="KEGG" id="sso:SSO2825"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2910"/>
    <dbReference type="eggNOG" id="arCOG07332">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140801_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V12"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="158" mass="17645" checksum="B74836CAAAD22175" modified="2001-10-01" version="1">MRKSNIGMITSAIIPAFTIVYQPIWLLGLIITSIASTKLFDPNFKDSIYSPNFRKNTSIYLLVLSILEGITGFGAGPQTSGIISTLTFNLLNRGNSLELHLVLIIPLALFFILHTVSGVGSLILSKGIKNPILFKYIIPIVWIMMYLVVVYLDLYYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V35</accession>
    <name>Q97V35_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Polysulphide reductase NrfD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2797</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NrfD family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90456">
        <property type="entry name" value="G90456"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923974.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V35"/>
    <dbReference type="STRING" id="273057.SSO2797"/>
    <dbReference type="PaxDb" id="273057-SSO2797"/>
    <dbReference type="EnsemblBacteria" id="AAK42910">
        <property type="protein sequence ID" value="AAK42910"/>
        <property type="gene ID" value="SSO2797"/>
    </dbReference>
    <dbReference type="GeneID" id="1452829"/>
    <dbReference type="KEGG" id="sso:SSO2797"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2883"/>
    <dbReference type="eggNOG" id="arCOG02027">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_779911_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V35"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1630.10">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052049">
        <property type="entry name" value="Electron_transfer_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005614">
        <property type="entry name" value="NrfD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34856">
        <property type="entry name" value="PROTEIN NRFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34856:SF2">
        <property type="entry name" value="PROTEIN NRFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03916">
        <property type="entry name" value="NrfD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="39198" checksum="E2AC411B948E5D98" modified="2001-10-01" version="1">MIKMGLFTAPEPGTSFGVQAPIIQINQYPLWGEYVALALYFTEIAGMLMAIIGLMEVTGKYKAFNRRASVAAFIASILALLFFDIDLGRPTAAILAPAEAFFYFAFSWMARGIIFVGGLLLFSFLYMVISLLDVKNRWARGVIAILGVFAGIFSTTYSGFELASTTGIPFWNNGGLPALYLADGVFAASGLGYLIALVGKNEEVMRARVTLTKVLFYSSIAILATWFLFLANVNYLNVFNQVAYEYLLSQVTFYIDIGLSVLTLIISGIGYMSITRFLMFGMRQKAPESAIGQMPVMDLPTVIKYVVVVAAIFALVAGFLARADVLFAGQYAYQLSPMTPFQSVSNQPIPIGSFGWRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V52</accession>
    <name>Q97V52_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VapB-type antitoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO11133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90454">
        <property type="entry name" value="F90454"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993330.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V52"/>
    <dbReference type="STRING" id="273057.SSO11133"/>
    <dbReference type="PaxDb" id="273057-SSO11133"/>
    <dbReference type="EnsemblBacteria" id="AAK42893">
        <property type="protein sequence ID" value="AAK42893"/>
        <property type="gene ID" value="SSO11133"/>
    </dbReference>
    <dbReference type="GeneID" id="1452812"/>
    <dbReference type="KEGG" id="sso:SSO11133"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2865"/>
    <dbReference type="eggNOG" id="arCOG05414">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_194227_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V52"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9531" checksum="1B8A11F1E8A5A41F" modified="2001-10-01" version="1">METVTIKVDKEIAELIKKMISLGIAKSKNEAVNMLIEYGRAEIERRVKEEEEVKKLVEKWLQEGFPYKNLDTSDLREERYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V78</accession>
    <name>Q97V78_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="4">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">porA-2</name>
        <name evidence="9" type="ordered locus">SSO2757</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90451">
        <property type="entry name" value="D90451"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990252.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V78"/>
    <dbReference type="FunCoup" id="Q97V78">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2757"/>
    <dbReference type="PaxDb" id="273057-SSO2757"/>
    <dbReference type="EnsemblBacteria" id="AAK42867">
        <property type="protein sequence ID" value="AAK42867"/>
        <property type="gene ID" value="SSO2757"/>
    </dbReference>
    <dbReference type="GeneID" id="1452786"/>
    <dbReference type="KEGG" id="sso:SSO2757"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2836"/>
    <dbReference type="eggNOG" id="arCOG01608">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002569_5_0_2"/>
    <dbReference type="InParanoid" id="Q97V78"/>
    <dbReference type="PhylomeDB" id="Q97V78"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000010">
        <property type="entry name" value="Pyruvate ferredoxin oxidoreductase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000012">
        <property type="entry name" value="Pyruvate:ferredoxin (Flavodoxin) oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF0">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="7">
        <location>
            <begin position="15"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate:ferredoxin oxidoreductase core" evidence="8">
        <location>
            <begin position="263"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="400" mass="44641" checksum="C3F333BFD8684185" modified="2001-10-01" version="1">MIRKIISGNEAVALGVKLARVGVTGIYPITPQTTIIEKLAEMRAKGEIETEIVRVESEHSAMAATFGAALGGVRAFTATASQGLLYMHEMVWWVAGSRVPVVMVVGTRAVGAPWNIWNEHTDFTSERDSGWIMAFASNPQEALDLTLQAFRISEDERVFLPMMIGMDGFILSHTKTNVLIPDQDQVDDFLPPRRQPYVIDPEDPVGMGNIFPPEGYMRLRESIDLALRNSEDVIKEIGREYNKKINPLGDYSTLNVSYKLEDADYAVVLMGAWAGDAMEAVDTLREKGIKIGMLRIRYLRPWSEKEIRNTLKDKKAVLVLDRSTSFGRGGGPLYVEIKSTISNVVPQIKGVVSGLGGVTVNKNDLVYIFNKFVEGLKDDVIWYYPKEVGKIELRTPRDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VD6</accession>
    <name>Q97VD6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acylaminoacyl-peptidase, putative (ApeH-3)</fullName>
            <ecNumber evidence="3">3.4.19.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">apeH-3</name>
        <name evidence="3" type="ordered locus">SSO2693</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.19.1" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99444">
        <property type="entry name" value="A99444"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988616.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VD6"/>
    <dbReference type="SMR" id="Q97VD6"/>
    <dbReference type="FunCoup" id="Q97VD6">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2693"/>
    <dbReference type="ESTHER" id="sulso-APEH3">
        <property type="family name" value="ACPH_Peptidase_S9"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2693"/>
    <dbReference type="EnsemblBacteria" id="AAK42808">
        <property type="protein sequence ID" value="AAK42808"/>
        <property type="gene ID" value="SSO2693"/>
    </dbReference>
    <dbReference type="GeneID" id="1452727"/>
    <dbReference type="KEGG" id="sso:SSO2693"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2774"/>
    <dbReference type="eggNOG" id="arCOG01646">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008615_2_2_2"/>
    <dbReference type="InParanoid" id="Q97VD6"/>
    <dbReference type="PhylomeDB" id="Q97VD6"/>
    <dbReference type="BRENDA" id="3.4.19.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF4">
        <property type="entry name" value="ACYLAMINO-ACID-RELEASING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="2">
        <location>
            <begin position="382"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="591" mass="67147" checksum="456477B3386BDF29" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="137" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VF4</accession>
    <name>Q97VF4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter, ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2672</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90441">
        <property type="entry name" value="G90441"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988590.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VF4"/>
    <dbReference type="FunCoup" id="Q97VF4">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2672"/>
    <dbReference type="TCDB" id="3.A.1.5.9">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2672"/>
    <dbReference type="EnsemblBacteria" id="AAK42790">
        <property type="protein sequence ID" value="AAK42790"/>
        <property type="gene ID" value="SSO2672"/>
    </dbReference>
    <dbReference type="GeneID" id="1452709"/>
    <dbReference type="KEGG" id="sso:SSO2672"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2754"/>
    <dbReference type="eggNOG" id="arCOG00184">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="InParanoid" id="Q97VF4"/>
    <dbReference type="PhylomeDB" id="Q97VF4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43230">
        <property type="entry name" value="ABC-TYPE DIPEPTIDE/OLIGOPEPTIDE TRANSPORT SYSTEM, ATPASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43230:SF3">
        <property type="entry name" value="ABC-TYPE DIPEPTIDE_OLIGOPEPTIDE TRANSPORT SYSTEM, ATPASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="324" mass="37181" checksum="B6B7BC9B55B06F75" modified="2001-10-01" version="1">MGLMELKGVSVIFEDKVGLFKKRKFYALKDVSLSMNQGDLLIVLGESGAGKTTLGRVIVGLQKPTSGEVVYDGYNIWKNKRKIFKKYRKDVQLIPQDPYSTLPFNKTVEEILVAPILRWEKINKDELRKRLINLLELVKLTPAEEFLGKYPHQLSGGQKQRLSIARSLSVNPRIIVADEPVTMVDASLRIGILNTLAEIKNRLNLTMVFITHDIPIARYFYHLFDKGNTIVMFAGRIVERADLEEILKDPLHPYTNDLIKLTPSIDNLYKEINVKINYERVEKGCPYRLRCPFAMDICKNEEPKLFKYSHEVACFLYGKVGESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VL9</accession>
    <name>Q97VL9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Small heat shock protein hsp20 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2603</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90433">
        <property type="entry name" value="F90433"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989320.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VL9"/>
    <dbReference type="SMR" id="Q97VL9"/>
    <dbReference type="STRING" id="273057.SSO2603"/>
    <dbReference type="PaxDb" id="273057-SSO2603"/>
    <dbReference type="EnsemblBacteria" id="AAK42725">
        <property type="protein sequence ID" value="AAK42725"/>
        <property type="gene ID" value="SSO2603"/>
    </dbReference>
    <dbReference type="GeneID" id="1454052"/>
    <dbReference type="KEGG" id="sso:SSO2603"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2685"/>
    <dbReference type="eggNOG" id="arCOG01832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_9_5_2"/>
    <dbReference type="InParanoid" id="Q97VL9"/>
    <dbReference type="PhylomeDB" id="Q97VL9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000117">
        <property type="entry name" value="Heat shock protein Hsp20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041799">
        <property type="entry name" value="Hsp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="19"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="124" mass="14145" checksum="2532C298F9E5443C" modified="2001-10-01" version="1">MMNVIMREIGKKLDELSREFYESVIPPIDMYEEGGELVVVADLAGFNKDKISVRLSAQNELIINAEREIQYIGTKYATQRPLKIHKVIRLPVKVKRDSQVTAKYENGVLTIRIPVEGSVSIRIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VP1</accession>
    <name>Q97VP1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90430">
        <property type="entry name" value="G90430"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991786.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VP1"/>
    <dbReference type="STRING" id="273057.SSO2575"/>
    <dbReference type="PaxDb" id="273057-SSO2575"/>
    <dbReference type="EnsemblBacteria" id="AAK42702">
        <property type="protein sequence ID" value="AAK42702"/>
        <property type="gene ID" value="SSO2575"/>
    </dbReference>
    <dbReference type="GeneID" id="1454029"/>
    <dbReference type="KEGG" id="sso:SSO2575"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2658"/>
    <dbReference type="eggNOG" id="arCOG06005">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022755_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VP1"/>
    <dbReference type="PhylomeDB" id="Q97VP1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024191">
        <property type="entry name" value="Peptidase_M61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007963">
        <property type="entry name" value="Peptidase_M61_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040756">
        <property type="entry name" value="Peptidase_M61_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05299">
        <property type="entry name" value="Peptidase_M61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17899">
        <property type="entry name" value="Peptidase_M61_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016493">
        <property type="entry name" value="Glycyl_aminpptds"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="1">
        <location>
            <begin position="392"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="506" mass="58757" checksum="AB084C80F24FD103" modified="2001-10-01" version="1">MRFYINPRNKYIEVYGEGREGIIIFPTWVPGSYVIRDTERYVVEIDGIRIGKNRFFVKDKFRYLIQALSKDQREAISTSDYLFINPASVFPFQTIEEEYCVKINVRWPIHTTLKKVGDWFCADNYNEFADSPIQASPKLKLIEIDRNHKISTIDELDKPIVESLGKCTFEIDQNIFSDSSTNEYIFFFRRSDTDFGGIEHERSSSIVISWNYKDLIRLFIHEYFHRYNVKKIRPKDLKINYESETYTELLWVAEGLTEYIAVIVPLRTKVVKVDDTLNYIANTLAWLTFPGIRRMSLAESSYTTWIKYYRRDNNFLNVGISYYQLGLIVGLIMDLEMIDSGNSIYNFFRELYKIREYTYGNVRDIAEGLGVQNIDELVFSRNPPIFNRLSKFFKLSFVDKGFPYCGLMLDNKKVTFVEDDSPADKAGIIPGDEIVGINGISSSNLNLECKDKLELVINREGRLIEFTIIPDGNPGHNLVIKGNGEIFKKWSGGIEYGEGKSNIRII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VR4</accession>
    <name>Q97VR4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2549</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90427">
        <property type="entry name" value="F90427"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VR4"/>
    <dbReference type="STRING" id="273057.SSO2549"/>
    <dbReference type="PaxDb" id="273057-SSO2549"/>
    <dbReference type="EnsemblBacteria" id="AAK42677">
        <property type="protein sequence ID" value="AAK42677"/>
        <property type="gene ID" value="SSO2549"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2549"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2629"/>
    <dbReference type="eggNOG" id="arCOG03464">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034270_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VR4"/>
    <dbReference type="PhylomeDB" id="Q97VR4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF7">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="505" mass="55865" checksum="8B49621E6C0C5ADD" modified="2001-10-01" version="1">MVGIRIMTKLFVRETSGLTKNVSLLDVTMLNVANMGAGLAIFIGISPYIVEGAVLWIASLLTFLFTLPLVFLYTYFISRIPRTGGDYIWLSRKLNGPLGAIMGVALAFNMPPFFALSAFFSVSAINSVLLTIGTLNSQPYLVNLANNVFTTSNAVLAYGLAAVAFLIIILINILRPRWGYTLTSALGMFSLLGTLVAILVLTLNISDFHTVIQPFIKDFNLTVQLYKGPTFSLSSTFYMIPYFASFAYIWLYAGPAVASEIKNKSGIRYNLILGSLLTLIFITVPFLLMDVAAGYGFNYSLYLSGTYNFWTVAIALSRNYALEWFIGISLIAWEFFVMAFGVVVFARYVFAFSFDRLFPEVFSRLNKQGSPAYAHLLDFVVTLVFLAVPIISPNGYEALYSYTPLAIAYLVLVSIAGIRQALSERNLGMLISALLSIGFLGFMGYEAFTNPYFGVISDNGVPYWPGIAYITSLIGLGVITFVSSYYYRKKSGIDLRLVYKEIPPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VR6</accession>
    <name>Q97VR6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90427">
        <property type="entry name" value="D90427"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989876.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VR6"/>
    <dbReference type="STRING" id="273057.SSO2546"/>
    <dbReference type="PaxDb" id="273057-SSO2546"/>
    <dbReference type="EnsemblBacteria" id="AAK42675">
        <property type="protein sequence ID" value="AAK42675"/>
        <property type="gene ID" value="SSO2546"/>
    </dbReference>
    <dbReference type="GeneID" id="1454002"/>
    <dbReference type="KEGG" id="sso:SSO2546"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2627"/>
    <dbReference type="eggNOG" id="arCOG07324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1197671_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VR6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="231" mass="26270" checksum="45F2D961858D1498" modified="2001-10-01" version="1">MSSPLWFLFVIVLFPHAFVEYSLHIQVDYNNQVYHLSELLFENVTKVYYSNSTFVYNLTVYVLNFSQILTSSAILDNFSLPKTFFYIPNPGEPVVERSVILSLLSSNDSNYIYYGKTFIGYVELEYFYYVNSTGVPYKIIILQIGESGQLVSNTTYILVKSNLVNPNEQPILPEGFKIANGSEVVNVPISLGLNSALDQLVGKYISFVTLLLVFVFLTVRFYASRKKTSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VT9</accession>
    <name>Q97VT9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (HpcE-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hpcE-1</name>
        <name evidence="2" type="ordered locus">SSO2520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90424">
        <property type="entry name" value="D90424"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988479.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VT9"/>
    <dbReference type="FunCoup" id="Q97VT9">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2520"/>
    <dbReference type="PaxDb" id="273057-SSO2520"/>
    <dbReference type="EnsemblBacteria" id="AAK42651">
        <property type="protein sequence ID" value="AAK42651"/>
        <property type="gene ID" value="SSO2520"/>
    </dbReference>
    <dbReference type="GeneID" id="1453978"/>
    <dbReference type="KEGG" id="sso:SSO2520"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2597"/>
    <dbReference type="eggNOG" id="arCOG00235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_3_3_2"/>
    <dbReference type="InParanoid" id="Q97VT9"/>
    <dbReference type="PhylomeDB" id="Q97VT9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43211:SF1">
        <property type="entry name" value="BLL6422 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43211">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="1">
        <location>
            <begin position="103"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="318" mass="36421" checksum="9D787F8F33E3D255" modified="2001-10-01" version="1">MKLISFIRDNRIRSGVLVNDEFIADLNTSCYLMLIEKGEEEQFAERYCNAIVPPDMLGVLQAGDKGFEVVKEILEWSKKRNDILYNINQVKLKAPLVRANMLRDFLAFRGHVEATYKRRGQPIPEEWFRIPIYYKGDPAIFYGHLEEVPWPKYSKLVDIELEIAAIIYKRGKDIDKIRARDYILGFTIFNDFSARDIQMAEMKGLLGPAKGKDFANGLGPWIVTKDELSDIKGLRVYAKVNNEIWCDTKAEDMQWSFEDMIAYVSQDEYIRPGDVFGSGTVSGCTGLDIGKSLKPNSSIELYVEKIGILKNIIVKNHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VW1</accession>
    <name>Q97VW1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Lipase (LipP-1)</fullName>
            <ecNumber evidence="4">3.1.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">lipP-1</name>
        <name evidence="4" type="ordered locus">SSO2493</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90421">
        <property type="entry name" value="F90421"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923897.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VW1"/>
    <dbReference type="SMR" id="Q97VW1"/>
    <dbReference type="FunCoup" id="Q97VW1">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2493"/>
    <dbReference type="ESTHER" id="sulir-f0ndq1">
        <property type="family name" value="Hormone-sensitive_lipase_like"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2493"/>
    <dbReference type="EnsemblBacteria" id="AAK42629">
        <property type="protein sequence ID" value="AAK42629"/>
        <property type="gene ID" value="SSO2493"/>
    </dbReference>
    <dbReference type="GeneID" id="7797386"/>
    <dbReference type="KEGG" id="sso:SSO2493"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2574"/>
    <dbReference type="eggNOG" id="arCOG02638">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_6_4_2"/>
    <dbReference type="InParanoid" id="Q97VW1"/>
    <dbReference type="PhylomeDB" id="Q97VW1"/>
    <dbReference type="BRENDA" id="3.1.1.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000089">
        <property type="entry name" value="Alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF8">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="74"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="305" mass="33433" checksum="332CCE8B411271D9" modified="2001-10-01" version="1">MPLDPRIKELLESGFIVPIGKASVDEVRKIFRQLASAAPKVEVGKVEDIKIPGSEANINARVYLPKANGPYGVLIYLHGGGFVIGDVESYDPLCRAITNACNCVVVSVDYRLAPEYKFPSAVIDSFDATNWVYNNLDKFDGKMGVAIAGDSAGGNLAAVVALLSKGKLNLKYQILIYPAVGFDSVSRSMIEYSDGFFLTREHIEWFGSQYLRSPADLLDFRFSPILVQDLSGLPPALIITAEYDPLRDQGEAYANRLLQAGVPVTSVRFNNVIHGFLSFFPLIEQGRDAIGLIGSVLRRAFYDKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VY3</accession>
    <name>LEUD_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>3-isopropylmalate dehydratase small subunit</fullName>
            <ecNumber>4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Alpha-IPM isomerase</fullName>
            <shortName>IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">leuD</name>
        <name type="ordered locus">SSO2470</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LeuD family. LeuD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90418">
        <property type="entry name" value="H90418"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923875.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VY3"/>
    <dbReference type="SMR" id="Q97VY3"/>
    <dbReference type="FunCoup" id="Q97VY3">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2470"/>
    <dbReference type="PaxDb" id="273057-SSO2470"/>
    <dbReference type="EnsemblBacteria" id="AAK42607">
        <property type="protein sequence ID" value="AAK42607"/>
        <property type="gene ID" value="SSO2470"/>
    </dbReference>
    <dbReference type="GeneID" id="7808220"/>
    <dbReference type="KEGG" id="sso:SSO2470"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2547"/>
    <dbReference type="eggNOG" id="arCOG02230">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081378_1_1_2"/>
    <dbReference type="InParanoid" id="Q97VY3"/>
    <dbReference type="PhylomeDB" id="Q97VY3"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01032">
        <property type="entry name" value="LeuD_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050075">
        <property type="entry name" value="LeuD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011827">
        <property type="entry name" value="LeuD_type2/HacB/DmdB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02087">
        <property type="entry name" value="LEUD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43345:SF2">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43345">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000141952" description="3-isopropylmalate dehydratase small subunit">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="165" mass="18046" checksum="644933D3B6105DEB" modified="2001-10-01" version="1">MIIEGPVIKFGDKIDTDIIIPARYLKYTDPQYLAQHVMEPLDPEFYKKASKGVIIVAGKVFGMGSSREQAAIALKAAGVKAVVAESFARIFYRNAINNGLPVITLPNSTKEIDENSYVKIDVETGEILVGNKVLKGKGITGMALEILQAGGIMEYLKKMQTVNRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W03</accession>
    <name>Q97W03_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2445</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90416">
        <property type="entry name" value="D90416"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993134.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W03"/>
    <dbReference type="STRING" id="273057.SSO2445"/>
    <dbReference type="PaxDb" id="273057-SSO2445"/>
    <dbReference type="EnsemblBacteria" id="AAK42587">
        <property type="protein sequence ID" value="AAK42587"/>
        <property type="gene ID" value="SSO2445"/>
    </dbReference>
    <dbReference type="GeneID" id="1453914"/>
    <dbReference type="KEGG" id="sso:SSO2445"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2525"/>
    <dbReference type="eggNOG" id="arCOG05929">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1773284_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W03"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="147" mass="16948" checksum="186B672CAF94BDF5" modified="2001-10-01" version="1">MKIKLKSLVRVIGEEELAVIPLAENEYYVECLNFYEDVEGGRQARLVVVVDKYGIIRQDQVNFIKGKKTFVDAIGVEDDFRKINSVLKLDRVARMFKVPLYFDIEIVEKPDVSKRGIRGLYNYLSVHKEIDIGKLRGLVNLSIEELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W19</accession>
    <name>Q97W19_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Small heat shock protein hsp20 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2427</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2015" name="Biochem. Biophys. Res. Commun." volume="0" first="0" last="0">
            <title>Crystal structure and function of an unusual dimeric Hsp20.1 provide insight into the thermal protection mechanism of small heat shock proteins.</title>
            <authorList>
                <person name="Liang L."/>
                <person name="Yun C.H."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.68 ANGSTROMS) OF 77-166</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90414">
        <property type="entry name" value="D90414"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989398.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="4RZK">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.68 A"/>
        <property type="chains" value="A/B=77-166"/>
    </dbReference>
    <dbReference type="PDBsum" id="4RZK"/>
    <dbReference type="AlphaFoldDB" id="Q97W19"/>
    <dbReference type="SMR" id="Q97W19"/>
    <dbReference type="FunCoup" id="Q97W19">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2427"/>
    <dbReference type="PaxDb" id="273057-SSO2427"/>
    <dbReference type="EnsemblBacteria" id="AAK42571">
        <property type="protein sequence ID" value="AAK42571"/>
        <property type="gene ID" value="SSO2427"/>
    </dbReference>
    <dbReference type="GeneID" id="1453898"/>
    <dbReference type="KEGG" id="sso:SSO2427"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2505"/>
    <dbReference type="eggNOG" id="arCOG01833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117605_1_0_2"/>
    <dbReference type="InParanoid" id="Q97W19"/>
    <dbReference type="PhylomeDB" id="Q97W19"/>
    <dbReference type="EvolutionaryTrace" id="Q97W19"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044587">
        <property type="entry name" value="HSP21-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041800">
        <property type="entry name" value="Hsp20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46733">
        <property type="entry name" value="26.5 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46733:SF4">
        <property type="entry name" value="HEAT SHOCK PROTEIN 21, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="8" id="KW-0002">3D-structure</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="77"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="CS" evidence="5">
        <location>
            <begin position="82"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="4RZK"/>
        </source>
    </evidence>
    <sequence length="176" mass="20097" checksum="7A4E5FDBEB4C3C4A" modified="2001-10-01" version="1">MPKREEKDIFDLMDEWIREMEEEFERIEREFMRGFRGKGEGIRQFGPYVYGFRITVGPDGVPKIEEFGNVRKIRGKPMISEEREPLADVIEKGDEIKVVAEVPGVNKEDIKVKVTNGGKKLVITAKSEDRQYYKEIDLPAEVDEKAAKANFKNGVLEITLKKKASSSDSGVDIKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W69</accession>
    <name>Q97W69_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">O-succinylhomoserine (Thiol)-lyase (Cystathionine gamma-synthase) (MetB)</fullName>
            <ecNumber evidence="4">2.5.1.48</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">metB</name>
        <name evidence="4" type="ordered locus">SSO2368</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.5.1.48" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90407">
        <property type="entry name" value="H90407"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989477.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W69"/>
    <dbReference type="FunCoup" id="Q97W69">
        <property type="interactions" value="239"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2368"/>
    <dbReference type="PaxDb" id="273057-SSO2368"/>
    <dbReference type="EnsemblBacteria" id="AAK42519">
        <property type="protein sequence ID" value="AAK42519"/>
        <property type="gene ID" value="SSO2368"/>
    </dbReference>
    <dbReference type="GeneID" id="1453846"/>
    <dbReference type="KEGG" id="sso:SSO2368"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2453"/>
    <dbReference type="eggNOG" id="arCOG00060">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_2_0_2"/>
    <dbReference type="InParanoid" id="Q97W69"/>
    <dbReference type="PhylomeDB" id="Q97W69"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003962">
        <property type="term" value="F:cystathionine gamma-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047804">
        <property type="term" value="F:cysteine-S-conjugate beta-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000046">
        <property type="entry name" value="Cystathionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000033">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005047">
        <property type="entry name" value="PRK06460.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF50">
        <property type="entry name" value="CYSTATHIONINE BETA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="376" mass="41458" checksum="A4F5225396F50D16" modified="2001-10-01" version="1">MKDATRAVREDIDDLTGAITTPIYQTSAYHYPEGEKYRYSRESNPTVLELAKKIVELENAEMGIAFSSGMGAISTTALALLKPGNSVLVHRDMFGRSYRFFTDYLKNWGVNVEASYPGNNNIIEKAKSKRYDVIFVENITNPLLRVIDVAELSKVAKEQGSILIVDATFSTPINQKPLELGADIVVHSASKFLAGHNDVIAGLAAGYSKYLNVIDQMRRTLGTSLDPHAAYLTLRGIKTLKIRMDVINRNAEQIAEFLEGHPKVVKVYYPGLKSHVDYDIARKVLKGFGGVLSFEVNGGQESALKVMKSLKLIIPAQTLGGVNSVISHPATMSHRTLSLEERKIVGITDSLLRLSVGIEDVNDLIEDLDRALSTLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W72</accession>
    <name>Q97W72_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PqqD family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO10285</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90407">
        <property type="entry name" value="E90407"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989482.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W72"/>
    <dbReference type="STRING" id="273057.SSO10285"/>
    <dbReference type="PaxDb" id="273057-SSO10285"/>
    <dbReference type="EnsemblBacteria" id="AAK42516">
        <property type="protein sequence ID" value="AAK42516"/>
        <property type="gene ID" value="SSO10285"/>
    </dbReference>
    <dbReference type="GeneID" id="65562749"/>
    <dbReference type="KEGG" id="sso:SSO10285"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2450"/>
    <dbReference type="eggNOG" id="arCOG03838">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166552_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W72"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1150">
        <property type="entry name" value="Coenzyme PQQ synthesis protein D (PqqD)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008792">
        <property type="entry name" value="PQQD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041881">
        <property type="entry name" value="PqqD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05402">
        <property type="entry name" value="PqqD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="10684" checksum="78CFF6D1823EC2F5" modified="2001-10-01" version="1">MNFEDVKDKKPKKIGELIDRSEESDNYIVKVAEDKVYELAPIAYYIWAMCDGNRSVDEIVNELSKEANLDISQVRDPVVMVLDELEKASLITF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2014-06-11" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W73</accession>
    <name>SSB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Single-stranded DNA binding protein Ssb</fullName>
            <shortName>SSB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">ssb</name>
        <name type="ordered locus">SSO2364</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="12118" last="12127">
            <title>Physical and functional interaction between the mini-chromosome maintenance-like DNA helicase and the single-stranded DNA binding protein from the crenarchaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Carpentieri F."/>
                <person name="De Felice M."/>
                <person name="De Falco M."/>
                <person name="Rossi M."/>
                <person name="Pisani F.M."/>
            </authorList>
            <dbReference type="PubMed" id="11821426"/>
            <dbReference type="DOI" id="10.1074/jbc.M200091200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH MCM</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Mol. Microbiol." volume="48" first="361" last="371">
            <title>An archaeal XPF repair endonuclease dependent on a heterotrimeric PCNA.</title>
            <authorList>
                <person name="Roberts J.A."/>
                <person name="Bell S.D."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="12675797"/>
            <dbReference type="DOI" id="10.1046/j.1365-2958.2003.03444.x"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2004" name="J. Biol. Chem." volume="279" first="33192" last="33198">
            <title>Reverse gyrase recruitment to DNA after UV light irradiation in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Napoli A."/>
                <person name="Valenti A."/>
                <person name="Salerno V."/>
                <person name="Nadal M."/>
                <person name="Garnier F."/>
                <person name="Rossi M."/>
                <person name="Ciaramella M."/>
            </authorList>
            <dbReference type="PubMed" id="15190074"/>
            <dbReference type="DOI" id="10.1074/jbc.m402619200"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="564" last="576">
            <title>Functional interaction of reverse gyrase with single-strand binding protein of the archaeon Sulfolobus.</title>
            <authorList>
                <person name="Napoli A."/>
                <person name="Valenti A."/>
                <person name="Salerno V."/>
                <person name="Nadal M."/>
                <person name="Garnier F."/>
                <person name="Rossi M."/>
                <person name="Ciaramella M."/>
            </authorList>
            <dbReference type="PubMed" id="15673717"/>
            <dbReference type="DOI" id="10.1093/nar/gki202"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>DNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2003" name="EMBO J." volume="22" first="2561" last="2570">
            <title>Insights into ssDNA recognition by the OB fold from a structural and thermodynamic study of Sulfolobus SSB protein.</title>
            <authorList>
                <person name="Kerr I.D."/>
                <person name="Wadsworth R.I."/>
                <person name="Cubeddu L."/>
                <person name="Blankenfeldt W."/>
                <person name="Naismith J.H."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="12773373"/>
            <dbReference type="DOI" id="10.1093/emboj/cdg272"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.20 ANGSTROMS) OF 1-119</scope>
        <scope>SUBUNIT</scope>
        <scope>DNA-BINDING</scope>
        <scope>MUTAGENESIS OF ILE-30; TRP-56; TRP-75 AND PHE-79</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3 6">Binds to ssDNA, binds approximately 5 nucleotides per monomer, binding may be slightly cooperative (PubMed:12675797). Stimulates the DNA helicase activity of MCM (PubMed:11821426). Inhibits the endonuclease activity of XPF (PubMed:12675797). Stimulates reverse gyrase from S.shibatae strain B12 (rgy) (PubMed:15673717).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Interacts with MCM (PubMed:11821426). Monomer in solution, may bind ssDNA as an array of monomers (PubMed:12773373).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Chromosome</location>
        </subcellularLocation>
        <text evidence="5">About half the protein is soluble and half is probably DNA-associated; distribution does not change upon UV irradiation.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90407">
        <property type="entry name" value="D90407"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989484.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1O7I">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.20 A"/>
        <property type="chains" value="A/B=1-119"/>
    </dbReference>
    <dbReference type="PDB" id="2MNA">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-114"/>
    </dbReference>
    <dbReference type="PDB" id="7WCG">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-114"/>
    </dbReference>
    <dbReference type="PDBsum" id="1O7I"/>
    <dbReference type="PDBsum" id="2MNA"/>
    <dbReference type="PDBsum" id="7WCG"/>
    <dbReference type="AlphaFoldDB" id="Q97W73"/>
    <dbReference type="BMRB" id="Q97W73"/>
    <dbReference type="SMR" id="Q97W73"/>
    <dbReference type="FunCoup" id="Q97W73">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2364"/>
    <dbReference type="PaxDb" id="273057-SSO2364"/>
    <dbReference type="EnsemblBacteria" id="AAK42515">
        <property type="protein sequence ID" value="AAK42515"/>
        <property type="gene ID" value="SSO2364"/>
    </dbReference>
    <dbReference type="GeneID" id="1453842"/>
    <dbReference type="GeneID" id="44128089"/>
    <dbReference type="KEGG" id="sso:SSO2364"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2449"/>
    <dbReference type="eggNOG" id="arCOG01510">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110881_2_0_2"/>
    <dbReference type="InParanoid" id="Q97W73"/>
    <dbReference type="PhylomeDB" id="Q97W73"/>
    <dbReference type="EvolutionaryTrace" id="Q97W73"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04491">
        <property type="entry name" value="SoSSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="DisProt" id="DP03224"/>
    <dbReference type="FunFam" id="2.40.50.140:FF:000588">
        <property type="entry name" value="Single-stranded DNA binding protein Ssb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048970">
        <property type="entry name" value="OB_Ssb-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051231">
        <property type="entry name" value="SOSS-B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005048">
        <property type="entry name" value="PRK06461.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356">
        <property type="entry name" value="OB FOLD NUCLEIC ACID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356:SF0">
        <property type="entry name" value="SOSS COMPLEX SUBUNIT B HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21473">
        <property type="entry name" value="OB_Ssb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0158">Chromosome</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000429325" description="Single-stranded DNA binding protein Ssb">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OB">
        <location>
            <begin position="19"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Slight decrease in ssDNA binding." evidence="4">
        <original>I</original>
        <variation>A</variation>
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Significantly weaker ssDNA binding." evidence="4">
        <original>W</original>
        <variation>A</variation>
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Slight decrease in ssDNA binding." evidence="4">
        <original>W</original>
        <variation>A</variation>
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Significantly weaker ssDNA binding." evidence="4">
        <original>F</original>
        <variation>A</variation>
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="5"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="28"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="49"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="57"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="turn" evidence="9">
        <location>
            <begin position="60"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="82"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="92"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="99"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="104"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11821426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12675797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="12773373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="15190074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="15673717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7">
        <source>
            <dbReference type="PubMed" id="12773373"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="1O7I"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="2MNA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="7WCG"/>
        </source>
    </evidence>
    <sequence length="148" mass="16138" checksum="84147186545E763D" modified="2001-10-01" version="1">MEEKVGNLKPNMESVNVTVRVLEASEARQIQTKNGVRTISEAIVGDETGRVKLTLWGKHAGSIKEGQVVKIENAWTTAFKGQVQLNAGSKTKIAEASEDGFPESSQIPENTPTAPQQMRGGGRGFRGGGRRYGRRGGRRQENEEGEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W83</accession>
    <name>Q97W83_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Geranylgeranyl hydrogenase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2353</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90406">
        <property type="entry name" value="B90406"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W83"/>
    <dbReference type="STRING" id="273057.SSO2353"/>
    <dbReference type="PaxDb" id="273057-SSO2353"/>
    <dbReference type="EnsemblBacteria" id="AAK42505">
        <property type="protein sequence ID" value="AAK42505"/>
        <property type="gene ID" value="SSO2353"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2353"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2439"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024648_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W83"/>
    <dbReference type="PhylomeDB" id="Q97W83"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054906">
        <property type="entry name" value="DGGGPL_red"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054715">
        <property type="entry name" value="GGR_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041080">
        <property type="entry name" value="DGGGPL_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF18">
        <property type="entry name" value="DIGERANYLGERANYLGLYCEROPHOSPHOLIPID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22578">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="126"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="398" mass="44952" checksum="B4CEB56D7BB2C19C" modified="2001-10-01" version="1">MITWGMPYPQGKELEEKVEGIRLYSPDMKTIWTVKGEGFEIDSPSYVQRLAKEARDRGVEILDLTTAMKPIVIGNKVEGAVLFNRRTNETIEAKAKITVDATGYSTSFRNKLPFEFPVTETLDDKDADIAYREVLNTKDEIEEYPYLRIFITQKASPGGYWWYFPKGPNKVNVGLGIQGGMGYPSIHEFYNKYVDYYAPDIDKNRLLVKGGALVPTRRPLATIVWDGVAVIGDSAFTVNPVHGGGKGSAMISAYCVGKAILNAFEVNDFSAKGLWDANECYIERYGAKQASLDLFRRFLQRLSDDEINYGMNRKVIREEDLLEASANGDLQLSTAEKAMRVIMALGKPSLLFKLKTVAEYMKKVKDVYKHFPAEPKDLMKWKHSVDSIILEFNKALEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WA6</accession>
    <name>Q97WA6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO10224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90403">
        <property type="entry name" value="C90403"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992901.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WA6"/>
    <dbReference type="STRING" id="273057.SSO10224"/>
    <dbReference type="PaxDb" id="273057-SSO10224"/>
    <dbReference type="EnsemblBacteria" id="AAK42482">
        <property type="protein sequence ID" value="AAK42482"/>
        <property type="gene ID" value="SSO10224"/>
    </dbReference>
    <dbReference type="GeneID" id="1453809"/>
    <dbReference type="KEGG" id="sso:SSO10224"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2415"/>
    <dbReference type="eggNOG" id="arCOG08375">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2930365_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WA6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="60" mass="6597" checksum="F7EE57401A8F6AD1" modified="2001-10-01" version="1">MKIYNLGRALVLTGIIELILASLFYSDKKVILSVLLGDYSYFCLLIGVIVIAVYGAITKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WB3</accession>
    <name>Q97WB3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2322</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90402">
        <property type="entry name" value="D90402"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992495.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WB3"/>
    <dbReference type="STRING" id="273057.SSO2322"/>
    <dbReference type="PaxDb" id="273057-SSO2322"/>
    <dbReference type="EnsemblBacteria" id="AAK42475">
        <property type="protein sequence ID" value="AAK42475"/>
        <property type="gene ID" value="SSO2322"/>
    </dbReference>
    <dbReference type="GeneID" id="1453802"/>
    <dbReference type="KEGG" id="sso:SSO2322"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2409"/>
    <dbReference type="eggNOG" id="arCOG07206">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1096748_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WB3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056538">
        <property type="entry name" value="ArlX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24151">
        <property type="entry name" value="ArlX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="71"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="252" mass="28041" checksum="CFAD8C57605D351D" modified="2001-10-01" version="1">MININLPELQQLVESPLFILLISISIPLAAFFISFFKIVLPRITRPKNIQTTQTASTTQNQQQQGVTSNSTKEIEELLKNLIGRMDKYQNDLVNVLNTSIEDLKQTLQKLNSSIEDAVLSLKSAQADSSSPFNIISEQSKGEQRPEGSKELKAVAEIVGTSNIDLSVFIRNCVLLEILDYEDSKLTALYELGYISADDMFIVNKIQSYIRTNNGKIRAKDLANIAMSVAESYSSVTAEMKKYLLVLEVGKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WD4</accession>
    <name>Q97WD4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2292</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90399">
        <property type="entry name" value="G90399"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991818.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WD4"/>
    <dbReference type="PaxDb" id="273057-SSO2292"/>
    <dbReference type="EnsemblBacteria" id="AAK42454">
        <property type="protein sequence ID" value="AAK42454"/>
        <property type="gene ID" value="SSO2292"/>
    </dbReference>
    <dbReference type="GeneID" id="1453781"/>
    <dbReference type="KEGG" id="sso:SSO2292"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2386"/>
    <dbReference type="eggNOG" id="arCOG03465">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034270_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WD4"/>
    <dbReference type="PhylomeDB" id="Q97WD4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF7">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="332"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="435"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="473"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="512" mass="56775" checksum="15F8CA7BF4CF95D4" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WD6</accession>
    <name>Q97WD6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Exodeoxyribonuclease</fullName>
            <ecNumber evidence="12">3.1.11.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">SSO2290</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="12"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90399">
        <property type="entry name" value="E90399"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991823.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WD6"/>
    <dbReference type="FunCoup" id="Q97WD6">
        <property type="interactions" value="192"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2290"/>
    <dbReference type="PaxDb" id="273057-SSO2290"/>
    <dbReference type="EnsemblBacteria" id="AAK42452">
        <property type="protein sequence ID" value="AAK42452"/>
        <property type="gene ID" value="SSO2290"/>
    </dbReference>
    <dbReference type="GeneID" id="1453779"/>
    <dbReference type="KEGG" id="sso:SSO2290"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2384"/>
    <dbReference type="eggNOG" id="arCOG02207">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027539_1_3_2"/>
    <dbReference type="InParanoid" id="Q97WD6"/>
    <dbReference type="PhylomeDB" id="Q97WD6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09073">
        <property type="entry name" value="ExoIII_AP-endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000054">
        <property type="entry name" value="Exodeoxyribonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00195">
        <property type="entry name" value="exoDNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF6">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="11">
        <location>
            <begin position="4"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="8">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="10">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="10">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK42452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="247" mass="28463" checksum="8D19870CC5C10F9D" modified="2001-10-01" version="1">MKIVSWNINGLRSALKKNLVNFLESNTFDIVMFQETRGDIVPLDFIMMGYEVISFPARRKGYSGVMTLTKIKPIKVIKGLQIKEFDDEGRTVSLELKDFYLINAYFPRAGDNLERLDFKLKFNNGIENFLLKLREVKPVILCGDFNIAHQSIDAAFSDPKIPGLTPQERAWFTHLLSLGFIDTFRYLHPNVRKYSWWSYMGNAREKNLGLRLDYCIVSEELKDSIKMADILTDVQGSDHAPIILELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WE1</accession>
    <name>Q97WE1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90398">
        <property type="entry name" value="H90398"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991830.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WE1"/>
    <dbReference type="STRING" id="273057.SSO2285"/>
    <dbReference type="PaxDb" id="273057-SSO2285"/>
    <dbReference type="EnsemblBacteria" id="AAK42447">
        <property type="protein sequence ID" value="AAK42447"/>
        <property type="gene ID" value="SSO2285"/>
    </dbReference>
    <dbReference type="GeneID" id="1453774"/>
    <dbReference type="KEGG" id="sso:SSO2285"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2378"/>
    <dbReference type="eggNOG" id="arCOG00284">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_453183_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WE1"/>
    <dbReference type="PhylomeDB" id="Q97WE1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051162">
        <property type="entry name" value="T4SS_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121:SF1">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJGR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="191"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="609" mass="68789" checksum="3FEE0B5F162B0563" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WE2</accession>
    <name>Q97WE2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NurA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90398">
        <property type="entry name" value="G90398"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991831.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WE2"/>
    <dbReference type="STRING" id="273057.SSO2284"/>
    <dbReference type="PaxDb" id="273057-SSO2284"/>
    <dbReference type="EnsemblBacteria" id="AAK42446">
        <property type="protein sequence ID" value="AAK42446"/>
        <property type="gene ID" value="SSO2284"/>
    </dbReference>
    <dbReference type="GeneID" id="1453773"/>
    <dbReference type="KEGG" id="sso:SSO2284"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2377"/>
    <dbReference type="eggNOG" id="arCOG00287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_755697_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WE2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018977">
        <property type="entry name" value="NurA_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09376">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00933">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NurA" evidence="1">
        <location>
            <begin position="65"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="373" mass="41530" checksum="B8EC493AF0F3057B" modified="2001-10-01" version="1">MPKKLELQVLLKDIISKYIVNYLSSSSNISPNIENLVGEDDNRGILISPLNEFIRTVSFEKHSQSLIYSLDGSSRSFISSKGIVSLASVVVSSTISPILGVYPPISGFPELDLKKPFLALASSAHQSPLIPFFYSSEYVTTLSLDGSFFTSVNSPEEIETEIRTILETEALKKIPNDGSIILDGPLIPPLIFLRSKPRDDILSLRLQAIKGRNVIGIVKRLDKSRLLISSLYKLSSKFMEKFRVDPSRYFSDESFILDLLKANLSPPYSPISLGPILRNIGNTPVYVNYLIYPLHPYVFKFAILRVESLNNDSGVVDQISSLKFTKDGIPFILAIADRTAKEITNAILKIIMTSLESMGVQASFYSKLEQVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WH6</accession>
    <name>Q97WH6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Purine nucleosidase, putative (IunH-2)</fullName>
            <ecNumber evidence="4">3.2.2.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">iunH-2</name>
        <name evidence="4" type="ordered locus">SSO2243</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Biochemistry" volume="51" first="4590" last="4599">
            <title>New determinants in the catalytic mechanism of nucleoside hydrolases from the structures of two isozymes from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Minici C."/>
                <person name="Cacciapuoti G."/>
                <person name="De Leo E."/>
                <person name="Porcelli M."/>
                <person name="Degano M."/>
            </authorList>
            <dbReference type="PubMed" id="22551416"/>
            <dbReference type="DOI" id="10.1021/bi300209g"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 1-306 IN COMPLEX WITH CA(2+)</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <dbReference type="EC" id="3.2.2.1" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90394">
        <property type="entry name" value="D90394"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991551.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3T8I">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B/C/D=1-306"/>
    </dbReference>
    <dbReference type="PDBsum" id="3T8I"/>
    <dbReference type="AlphaFoldDB" id="Q97WH6"/>
    <dbReference type="SMR" id="Q97WH6"/>
    <dbReference type="FunCoup" id="Q97WH6">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2243"/>
    <dbReference type="PaxDb" id="273057-SSO2243"/>
    <dbReference type="EnsemblBacteria" id="AAK42411">
        <property type="protein sequence ID" value="AAK42411"/>
        <property type="gene ID" value="SSO2243"/>
    </dbReference>
    <dbReference type="GeneID" id="1453738"/>
    <dbReference type="KEGG" id="sso:SSO2243"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2338"/>
    <dbReference type="eggNOG" id="arCOG04558">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036838_2_2_2"/>
    <dbReference type="InParanoid" id="Q97WH6"/>
    <dbReference type="PhylomeDB" id="Q97WH6"/>
    <dbReference type="BRENDA" id="3.2.2.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97WH6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008477">
        <property type="term" value="F:purine nucleosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006152">
        <property type="term" value="P:purine nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02649">
        <property type="entry name" value="nuc_hydro_CeIAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.245.10:FF:000021">
        <property type="entry name" value="Purine nucleosidase, putative (IunH-2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015910">
        <property type="entry name" value="I/U_nuclsd_hydro_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052775">
        <property type="entry name" value="IUN_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190:SF1">
        <property type="entry name" value="SI:CH211-201H21.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190">
        <property type="entry name" value="SI:CH211-201H21.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01247">
        <property type="entry name" value="IUNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="6" id="KW-0002">3D-structure</keyword>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="3">
        <location>
            <begin position="4"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="6">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="3T8I"/>
        </source>
    </evidence>
    <sequence length="307" mass="34325" checksum="F213364EB1A4060F" modified="2001-10-01" version="1">MRKVIVDSDTATDDTIAILLASRFFQLLGVTIVAGNVNYNQEVKNALFTLEYIGKQDVPVYLGSQRPILGNWRTVEEVHGSNGMSNWNYPEPNKRPEKEHAIDAILRLSKEHEGELEILAISPLTNIALAYLKDPSVVKRVKKIWIMGGAFSKGNTTPIAEFNFWVDPEAAKIVLDAGFDITIVPWEVAEISGSLNERDWEYISKLNTKLSNFFINVNKTLKEYTTKNQGISGSIHPDSLTVSIAHDNSIILDSSLKYVDVELCSKSRGAMLIDWYSLHKNKPNAEIVLKADGGKFKNLLFNSLSQL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-03" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WJ5</accession>
    <accession>Q9UXN1</accession>
    <name>FENR_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ferredoxin--NADP reductase</fullName>
            <shortName evidence="1">FNR</shortName>
            <shortName evidence="1">Fd-NADP(+) reductase</shortName>
            <ecNumber evidence="1">1.18.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2222</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="1999-07" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Arcari P."/>
                <person name="Masullo M."/>
                <person name="Masullo L."/>
                <person name="Bocchini V."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 reduced [2Fe-2S]-[ferredoxin] + NADP(+) + H(+) = 2 oxidized [2Fe-2S]-[ferredoxin] + NADPH</text>
            <dbReference type="Rhea" id="RHEA:20125"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.18.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ferredoxin--NADP reductase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.2" evidence="1"/>
    <dbReference type="EMBL" id="AJ243598">
        <property type="protein sequence ID" value="CAB57297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90391">
        <property type="entry name" value="H90391"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992058.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WJ5"/>
    <dbReference type="SMR" id="Q97WJ5"/>
    <dbReference type="STRING" id="273057.SSO2222"/>
    <dbReference type="PaxDb" id="273057-SSO2222"/>
    <dbReference type="EnsemblBacteria" id="AAK42391">
        <property type="protein sequence ID" value="AAK42391"/>
        <property type="gene ID" value="SSO2222"/>
    </dbReference>
    <dbReference type="GeneID" id="1453718"/>
    <dbReference type="KEGG" id="sso:SSO2222"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2318"/>
    <dbReference type="eggNOG" id="arCOG01296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_5_2"/>
    <dbReference type="InParanoid" id="Q97WJ5"/>
    <dbReference type="PhylomeDB" id="Q97WJ5"/>
    <dbReference type="SABIO-RK" id="Q97WJ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004324">
        <property type="term" value="F:ferredoxin-NADP+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01685">
        <property type="entry name" value="FENR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022890">
        <property type="entry name" value="Fd--NADP_Rdtase_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000364991" description="Ferredoxin--NADP reductase">
        <location>
            <begin position="1"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; CAB57297." evidence="2" ref="2">
        <original>F</original>
        <variation>L</variation>
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="332" mass="36542" checksum="08E580F13F114A08" modified="2001-10-01" version="1">MDEYDIVVIGGGPVGLFGTFYAGLRDMKTLLIDAQDELGGQLVSLYPEKIVYDVGGLAGIQAYELAQRLIEQAKMFGPDIKVNELADMIEKTNDNMWIVKTDKATYKTKTIFIAAGIGKIVPSRLGAKGEIEYENRGVYYTVRRKKDFEGKRVLIVGGGDSAVDWALTLAPVAKSVTLIHRRDQFRAHERSVKELFRVANVYVWHELKEVKGDGNKVTQAIIFDNRTKEEKVLDVDSVIISIGYKGDLGNIPKWGVTMKGRDIVVNGRMETNLPGVYAGGDIVQMEGSPKLALIAVGFAHAAIAISVAKKYVEPNASLFAGHSSEMDKFKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WM2</accession>
    <name>Q97WM2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gamma-glutamylcyclotransferase AIG2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamylcyclotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90388">
        <property type="entry name" value="E90388"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992104.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WM2"/>
    <dbReference type="SMR" id="Q97WM2"/>
    <dbReference type="STRING" id="273057.SSO2192"/>
    <dbReference type="PaxDb" id="273057-SSO2192"/>
    <dbReference type="DNASU" id="1453691"/>
    <dbReference type="EnsemblBacteria" id="AAK42364">
        <property type="protein sequence ID" value="AAK42364"/>
        <property type="gene ID" value="SSO2192"/>
    </dbReference>
    <dbReference type="GeneID" id="1453691"/>
    <dbReference type="KEGG" id="sso:SSO2192"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2287"/>
    <dbReference type="eggNOG" id="arCOG03271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085350_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WM2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061929">
        <property type="term" value="F:gamma-glutamylaminecyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039126">
        <property type="entry name" value="GGACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12510:SF4">
        <property type="entry name" value="GAMMA-GLUTAMYLAMINECYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12510">
        <property type="entry name" value="TROPONIN C-AKIN-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-glutamylcyclotransferase AIG2-like" evidence="2">
        <location>
            <begin position="4"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma-glutamylcyclotransferase AIG2-like" evidence="2">
        <location>
            <begin position="145"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="276" mass="32414" checksum="2765BB196C922CD8" modified="2001-10-01" version="1">MYLLVYGSLRYGFELNHLVAKSRFVGLGYIEGYEMYDLGNYPGVIKGDGIVWGEVYEIDENLIKLLDEVEDYKGSPDDLYVREKTRVYFDQKRKYYLDNVYFYRYNKEIVKNMEKIEDGDYSAWSGMPIIVNYFAYAENTNERILHQRGVDMILSETPAYTKGYRLIFNIPCKYGYCANLIEDESGIICGYLQKVFLHTLNSLDKAEQHLIKYIREVVKVYDRNGKEYFAYAYVSSYKENRKKPSEEYVKIIREGLMRGWGNECVSTGLYDEPGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WT5</accession>
    <name>Q97WT5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99370">
        <property type="entry name" value="A99370"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WT5"/>
    <dbReference type="STRING" id="273057.SSO2029"/>
    <dbReference type="PaxDb" id="273057-SSO2029"/>
    <dbReference type="DNASU" id="1453543"/>
    <dbReference type="EnsemblBacteria" id="AAK42216">
        <property type="protein sequence ID" value="AAK42216"/>
        <property type="gene ID" value="SSO2029"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2029"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2109"/>
    <dbReference type="eggNOG" id="arCOG06009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1163847_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WT5"/>
    <dbReference type="PhylomeDB" id="Q97WT5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="212" mass="23340" checksum="7781F276F95FE1A7" modified="2001-10-01" version="1">MCQKLNMAFNGIKFDTSVPGMVPWEIVTIYFIVGLAIVFYFARRFGLKSFTTIDLVYIAVGAAFSVVWEFYIGSFIGRFLPSTPFIGVGFWGRMFILLIVAALVRKPGTGILSLLIFNILSDLFFYGFGGEPMYTIYEALTYGLFLDLVIVASRGKLFGIGYKSSNGSSVSTKTVVGLAILEGIIIGILFAIPDPIFYQFQTQSFILASLGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WU0</accession>
    <name>Q97WU0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase FkbM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90369">
        <property type="entry name" value="C90369"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923732.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WU0"/>
    <dbReference type="STRING" id="273057.SSO2024"/>
    <dbReference type="PaxDb" id="273057-SSO2024"/>
    <dbReference type="EnsemblBacteria" id="AAK42210">
        <property type="protein sequence ID" value="AAK42210"/>
        <property type="gene ID" value="SSO2024"/>
    </dbReference>
    <dbReference type="GeneID" id="1453537"/>
    <dbReference type="KEGG" id="sso:SSO2024"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2101"/>
    <dbReference type="eggNOG" id="arCOG01401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179025_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WU0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase FkbM" evidence="1">
        <location>
            <begin position="11"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="102" mass="11645" checksum="9A8BBE1E432082A1" modified="2001-10-01" version="1">MEYDDMTSAYESKGNKIVVDAVTLDSLGLKKVKLLKIDVERGELEVLKGTTNTLDITDKILIEVRKELEKDINSLLRAKGFKLVKVYMTYDNIGNFLYKRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WV3</accession>
    <name>Q97WV3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-methyl hydantoinase B (HyuB-2)</fullName>
            <ecNumber evidence="2">3.5.2.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hyuB-2</name>
        <name evidence="2" type="ordered locus">SSO2010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.14" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90367">
        <property type="entry name" value="F90367"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923720.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WV3"/>
    <dbReference type="STRING" id="273057.SSO2010"/>
    <dbReference type="PaxDb" id="273057-SSO2010"/>
    <dbReference type="EnsemblBacteria" id="AAK42197">
        <property type="protein sequence ID" value="AAK42197"/>
        <property type="gene ID" value="SSO2010"/>
    </dbReference>
    <dbReference type="GeneID" id="1453524"/>
    <dbReference type="KEGG" id="sso:SSO2010"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2084"/>
    <dbReference type="eggNOG" id="arCOG01512">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020413_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WV3"/>
    <dbReference type="PhylomeDB" id="Q97WV3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047423">
        <property type="term" value="F:N-methylhydantoinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF23">
        <property type="entry name" value="HYPOTHETICAL 5-OXOPROLINASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="510" mass="55614" checksum="BDE155D0D18E581D" modified="2001-10-01" version="1">MTSWEIIHKAFEFIAEEMGVMLKRSAMSPNIRERMDHSCAILDAEGNIIAQAEHIPVHLGSFSVGVKNTLKIVNLEEGDMVILNDPYISGTHLNDVMLLAPIYYNGKLIGYVVNKAHHVDVGGPSPGSLNPYATTIYEEGLVIPPVKIIKKGKLNEEIVEIIRENFKVPEVSLGDLNAQISANLNGVLRVKQLIDKYGLDRVLSSWAKSIEYGRSLALSEISKWNEGSFEDEDYLELDTLKRIRVKITISDKGIIADFNGTDNQVEAPFNAVYGVTFSAVSFVVRSLIGKDIPTNEGFYSVIKVIAPEGTIVNPLKPAAVGGGNVETSQRIADVTFKAFSHSIPVPAASSGTMMNVMMGGTYKGKYWAYYETIGGGMGGRPNKDGVSGVHVNMTNTLNTPIEIAERHYPILFTTYRIREGSGGKGMYRGGDGIIRAFKVLEKTKLSIMAERFKVPPWGLKGGENGKPGRVTIIRKGNVVEEMPSKFSTILNPGDEVIIETPGGGGYGSEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WW2</accession>
    <name>CAS5C_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated protein Cas5 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cas5c</name>
        <name type="ordered locus">SSO1998</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the aCascade ribonucleoprotein complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated protein Cas5 family. Subtype I-A/Apern subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90366">
        <property type="entry name" value="E90366"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991627.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WW2"/>
    <dbReference type="SMR" id="Q97WW2"/>
    <dbReference type="STRING" id="273057.SSO1998"/>
    <dbReference type="PaxDb" id="273057-SSO1998"/>
    <dbReference type="EnsemblBacteria" id="AAK42188">
        <property type="protein sequence ID" value="AAK42188"/>
        <property type="gene ID" value="SSO1998"/>
    </dbReference>
    <dbReference type="GeneID" id="7806460"/>
    <dbReference type="KEGG" id="sso:SSO1998"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2075"/>
    <dbReference type="eggNOG" id="arCOG02670">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154391_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WW2"/>
    <dbReference type="PhylomeDB" id="Q97WW2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09753">
        <property type="entry name" value="Cas5_I-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013422">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010153">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5a-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053725">
        <property type="entry name" value="CRISPR_Cas5_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01874">
        <property type="entry name" value="cas_cas5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02593">
        <property type="entry name" value="CRISPR_cas5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000417894" description="CRISPR-associated protein Cas5 3">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="240" mass="27211" checksum="30B5FA2A8A3A9A58" modified="2001-10-01" version="1">MIYSKVFLKLHWGFSVVKPSAAKAKSGFYLPPPTTLIGALSYGKFRGIDNTNLGKIYGSPAYNFRNVMATARLESEGAYTEDIVRNVISYFQRKDRRDNPRYIYGVIPTGKVYMPNGTLVVVYVTDSMSKEELEKLSWSITRIGGKECLVSVENVEIGEAKKVSGRMKTRYYFRDTVKVVGKKEFLEYVTFWEENGYIWGKEGGPIRYILPVATYPLASKEVEVEAKEAYEVGGEYVVFS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-25" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X14</accession>
    <name>THIC2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphomethylpyrimidine synthase 2</fullName>
            <ecNumber evidence="1">4.1.99.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Hydroxymethylpyrimidine phosphate synthase 2</fullName>
            <shortName evidence="1">HMP-P synthase 2</shortName>
            <shortName evidence="1">HMP-phosphate synthase 2</shortName>
            <shortName evidence="1">HMPP synthase 2</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thiamine biosynthesis protein ThiC 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiC2</name>
        <name type="synonym">thiC-2</name>
        <name type="ordered locus">SSO1941</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + CO + 5'-deoxyadenosine + formate + L-methionine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:24840"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.99.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.17" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90359">
        <property type="entry name" value="H90359"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992597.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X14"/>
    <dbReference type="SMR" id="Q97X14"/>
    <dbReference type="FunCoup" id="Q97X14">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1941"/>
    <dbReference type="PaxDb" id="273057-SSO1941"/>
    <dbReference type="EnsemblBacteria" id="AAK42135">
        <property type="protein sequence ID" value="AAK42135"/>
        <property type="gene ID" value="SSO1941"/>
    </dbReference>
    <dbReference type="GeneID" id="7798836"/>
    <dbReference type="KEGG" id="sso:SSO1941"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2015"/>
    <dbReference type="eggNOG" id="arCOG02741">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013181_2_2_2"/>
    <dbReference type="InParanoid" id="Q97X14"/>
    <dbReference type="PhylomeDB" id="Q97X14"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070284">
        <property type="term" value="F:phosphomethylpyrimidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="entry name" value="Radical SAM ThiC family, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00089">
        <property type="entry name" value="ThiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037509">
        <property type="entry name" value="ThiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009895">
        <property type="entry name" value="PRK13352.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557:SF1">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="THIAMINE BIOSYNTHESIS PROTEIN THIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01114">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00113">
        <property type="entry name" value="Radical_SAM_Phosphomethylpyrim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000152875" description="Phosphomethylpyrimidine synthase 2">
        <location>
            <begin position="1"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="184"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="225"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00089"/>
        </source>
    </evidence>
    <sequence length="433" mass="47641" checksum="73310287145B2B70" modified="2001-10-01" version="1">MGIIDEAKRGQITDEMRAISKLEGIPVEKVRNRISEGKIMLIRNAKYPSRKLVPIGKGLTTKVNVNIGTSSEVVDLDMELQKVKVANKWGDTLMDLSTGGDLDAIRRDIIKASDLPVGTVPVYQIFIESFKKKSGGAYFTEDELLNTVEKHLKDGVAFMTIHAGITKDLAIRALKSDRIIPIVSRGGDMIAGWMIHNNSENPYRKNWDYVLEMFKEYDAVISLGDALRPGATGDAHDEFQIGELLETARLVKSALQKGVQVMVEGPGHVPLNEIAWDVKLMKKLTGGVPYYVLGPLPIDVGAPYDHIASAIGAAISSASGVDLLCYLTPAEHLGLPTVKQVEEGAIAYRIAAHAGDVVKLGRKARKWDDEVSYYRGKLDWENMISKLIDPQRAYQVYTQFGTPKVKACTMCGGYCPMMWAMDQVRKIGSSSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X18</accession>
    <name>Q97X18_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Antibiotic ABC transporter ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1934</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90359">
        <property type="entry name" value="C90359"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923662.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X18"/>
    <dbReference type="FunCoup" id="Q97X18">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1934"/>
    <dbReference type="PaxDb" id="273057-SSO1934"/>
    <dbReference type="EnsemblBacteria" id="AAK42130">
        <property type="protein sequence ID" value="AAK42130"/>
        <property type="gene ID" value="SSO1934"/>
    </dbReference>
    <dbReference type="GeneID" id="1453457"/>
    <dbReference type="KEGG" id="sso:SSO1934"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2009"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q97X18"/>
    <dbReference type="PhylomeDB" id="Q97X18"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF18">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="297" mass="33638" checksum="DE50BF7BC656FD17" modified="2001-10-01" version="1">MIVVSSIVKKYGNKIALDNISLEVKKGEFVSLIGPNGAGKTTLIRILLTLMKPDKGEVQILGENPFRNKRIFKAIGYVQEIPNYPPFLTGRQVLELSAKIRGVDKSEVKRVLEFVEMENYANSPIIKYSKGMVQRIAIAESLLGNPSILIMDEPNMGIDPIFSLKTRELLNKLKRKDNVSILMTSHELEDVKKLSDRIFMIYKGKIVFSGTVEDMIKEFLGIQVIVETDEIENAEEALKGIEYVKGVFNDGNRLIVKLNEDRREDLLRDLVTSGVKVKGFYIDLNLEEAYMRALRNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X50</accession>
    <name>Q97X50_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Allantoin permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1905</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90355">
        <property type="entry name" value="B90355"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923643.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X50"/>
    <dbReference type="FunCoup" id="Q97X50">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1905"/>
    <dbReference type="PaxDb" id="273057-SSO1905"/>
    <dbReference type="EnsemblBacteria" id="AAK42097">
        <property type="protein sequence ID" value="AAK42097"/>
        <property type="gene ID" value="SSO1905"/>
    </dbReference>
    <dbReference type="GeneID" id="1453424"/>
    <dbReference type="KEGG" id="sso:SSO1905"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1966"/>
    <dbReference type="eggNOG" id="arCOG03448">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021555_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X50"/>
    <dbReference type="PhylomeDB" id="Q97X50"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015851">
        <property type="term" value="P:nucleobase transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11485">
        <property type="entry name" value="SLC-NCS1sbd_YbbW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000017">
        <property type="entry name" value="Permease for cytosine/purines uracil thiamine allantoin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF0">
        <property type="entry name" value="PURINE-URACIL PERMEASE NCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="434"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="488" mass="54171" checksum="60A8E3C9B0A0587F" modified="2001-10-01" version="1">MSDQEINHVNLDLTEYNQGRAVVPDNYYNPNIAPLSKNSKTWTWVNYTTIWAGMIHNVPAFMLAGLLTFEFGPLIALTVIAIAYFTLLIALYLNGHIGVKWGVPFPSSIRPMFGIRGARIPVIMRAISALFWFSVETYAGGLILDALISIFYPLWSTFSVNLLGMPLHMALSFFLFWLLNVLVLFKGMDEIKKFELIAGPLVIIILGGLMIHVATLANGLSPLFQIKASNVSLPNIALGISTMAGFWATLVLNIPDFTRFSRSQKDQLIRQAIGLPILTLLFSFIAVGLTSAVIYIYNIPSSEAINYVNPVNIMFLFTNNPYITLVLGISLVIATISVNVAANIVSPVYDLISLFPKRLNTWSKSAIVSAILGLLYSPWLWYNNASSIENVINLIGAGLGSVAGVMIAHYWILEKTEIKLAELFKPNGRYWYVSGYNINALIAMVIGFSVPVIGFLVPQLALLYDYGWYLGLFLSLMIYLGLEKKRIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X65</accession>
    <name>Q97X65_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thermopsin, probable</fullName>
            <ecNumber evidence="2">3.4.23.42</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.23.42" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90352">
        <property type="entry name" value="G90352"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990998.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X65"/>
    <dbReference type="STRING" id="273057.SSO1886"/>
    <dbReference type="PaxDb" id="273057-SSO1886"/>
    <dbReference type="EnsemblBacteria" id="AAK42078">
        <property type="protein sequence ID" value="AAK42078"/>
        <property type="gene ID" value="SSO1886"/>
    </dbReference>
    <dbReference type="GeneID" id="1453405"/>
    <dbReference type="KEGG" id="sso:SSO1886"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1943"/>
    <dbReference type="eggNOG" id="arCOG03670">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_494031_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X65"/>
    <dbReference type="PhylomeDB" id="Q97X65"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007981">
        <property type="entry name" value="Peptidase_A5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05317">
        <property type="entry name" value="Thermopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="533"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="556" mass="61461" checksum="6A8DA0438FCB003C" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XE0</accession>
    <name>Q97XE0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF973 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90343">
        <property type="entry name" value="B90343"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992016.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XE0"/>
    <dbReference type="PaxDb" id="273057-SSO1805"/>
    <dbReference type="EnsemblBacteria" id="AAK42001">
        <property type="protein sequence ID" value="AAK42001"/>
        <property type="gene ID" value="SSO1805"/>
    </dbReference>
    <dbReference type="GeneID" id="1454780"/>
    <dbReference type="KEGG" id="sso:SSO1805"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1863"/>
    <dbReference type="eggNOG" id="arCOG03778">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1485919_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XE0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="19623" checksum="3ACB594B785FDFF9" modified="2001-10-01" version="1">MNGRFLLQGNVISFIASIIILYGLILLLENGIYFALSKTFLILITFVWILAIPSYLSYRRSGLRKQWILNYFAIPAIVITLIGMILAYMGNFLGIEVIVLGYIFEPIAGISIYLNTLSFSKIYSSLFFWGALLFTIGLPLYLTNLGIVAVIGDVIKIVGIVGLINIGRKTYLTKPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XE3</accession>
    <name>Q97XE3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1803</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90342">
        <property type="entry name" value="G90342"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923599.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XE3"/>
    <dbReference type="PaxDb" id="273057-SSO1803"/>
    <dbReference type="EnsemblBacteria" id="AAK41998">
        <property type="protein sequence ID" value="AAK41998"/>
        <property type="gene ID" value="SSO1803"/>
    </dbReference>
    <dbReference type="GeneID" id="1454777"/>
    <dbReference type="KEGG" id="sso:SSO1803"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1859"/>
    <dbReference type="eggNOG" id="arCOG06508">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134214_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XE3"/>
    <dbReference type="PhylomeDB" id="Q97XE3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase InsH N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="169" mass="19632" checksum="E7B5C87583BF38D3" modified="2001-10-01" version="1">MVKAISTEKDLLLKVDKSFPWETFRSKLKSLYSKKPKWNVISLLKVLLIKLIFDISWNNLEGEIRDSKMFMDFLGGKIPPKSTVFSFYKKLQQTVIQEGETMRTTLMDELNKALDKAISEYREKGFELEVGREKKTEKVSKGTYPPKIDTFLPVKPYPREKRSLSKTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XH7</accession>
    <name>Q97XH7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hemerythrin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1764</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90337">
        <property type="entry name" value="F90337"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989074.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XH7"/>
    <dbReference type="STRING" id="273057.SSO1764"/>
    <dbReference type="PaxDb" id="273057-SSO1764"/>
    <dbReference type="EnsemblBacteria" id="AAK41957">
        <property type="protein sequence ID" value="AAK41957"/>
        <property type="gene ID" value="SSO1764"/>
    </dbReference>
    <dbReference type="GeneID" id="1454736"/>
    <dbReference type="KEGG" id="sso:SSO1764"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1811"/>
    <dbReference type="eggNOG" id="arCOG05367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1801781_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XH7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.520">
        <property type="entry name" value="nmb1532 protein domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="146" mass="17059" checksum="039AA714C98B5E8D" modified="2001-10-01" version="1">MLSLVLTLDHRRLEGLIDELLANPNLSLYGVIWKAYKNHIYWEEEILFKRVTDTSLLAIIRGLETEHGSMWILLKQTEELLRSNEIEGAREKIREFMRVLLEHDGAEEGSIYQFLESLSDEEQARLILEDIALAEPPRDWKCHAIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-01-24" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XK0</accession>
    <name>Q97XK0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial transposase ISC1359</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90334">
        <property type="entry name" value="E90334"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XK0"/>
    <dbReference type="PaxDb" id="273057-SSO1735"/>
    <dbReference type="EnsemblBacteria" id="AAK41932">
        <property type="protein sequence ID" value="AAK41932"/>
        <property type="gene ID" value="SSO1735"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1735"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1776"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067987_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XK0"/>
    <dbReference type="PhylomeDB" id="Q97XK0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033541">
        <property type="entry name" value="transpos_ISH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="49"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="310" mass="35943" checksum="5D1B2CD7BD8FEB8C" modified="2001-10-01" version="1">MLKASGSYLSRLGKDGRRALRKLNAVQVEDVRDALRKMGRMTLRGVRDRRVAVDFHAIPQYHADKSFLSRIKPTKGTSWGLVQAAIFLLGRTRSFLDVIPVTVKNVAEGFKAVMEVIVKELEEDKLRLVMVFADKEFAVNEVIRYLLELGLDFVISAKAQMYKKYKGMLQDVDVSFGGVRYTGFLCVRHGSGAYLIILRKEDGKSIAFLVRREMDLYDAIVLAEMYRERWGIENAFRSLEEFRIRTRTCDVRKELVLVLLSYLLLNVWFLIRSWRKVKLWEFSVSLSNLLDREVRVEQGRAFREVKTSFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XM1</accession>
    <name>Q97XM1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Amine oxidase</fullName>
            <ecNumber evidence="12">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">tynA</name>
        <name evidence="16" type="ordered locus">SSO1692</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a primary methyl amine + O2 + H2O = an aldehyde + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16153"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:228804"/>
            <dbReference type="EC" id="1.4.3.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="12">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="12">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="12"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90330">
        <property type="entry name" value="G90330"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990204.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XM1"/>
    <dbReference type="FunCoup" id="Q97XM1">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1692"/>
    <dbReference type="PaxDb" id="273057-SSO1692"/>
    <dbReference type="EnsemblBacteria" id="AAK41902">
        <property type="protein sequence ID" value="AAK41902"/>
        <property type="gene ID" value="SSO1692"/>
    </dbReference>
    <dbReference type="GeneID" id="1454681"/>
    <dbReference type="KEGG" id="sso:SSO1692"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1744"/>
    <dbReference type="eggNOG" id="arCOG07267">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011500_3_2_2"/>
    <dbReference type="InParanoid" id="Q97XM1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049947">
        <property type="entry name" value="Cu_Am_Ox_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049948">
        <property type="entry name" value="Cu_Am_ox_TPQ-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015800">
        <property type="entry name" value="Cu_amine_oxidase_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015802">
        <property type="entry name" value="Cu_amine_oxidase_N3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008559">
        <property type="entry name" value="PRK11504.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF86">
        <property type="entry name" value="COPPER AMINE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02727">
        <property type="entry name" value="Cu_amine_oxidN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02728">
        <property type="entry name" value="Cu_amine_oxidN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01164">
        <property type="entry name" value="COPPER_AMINE_OXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01165">
        <property type="entry name" value="COPPER_AMINE_OXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0801">TPQ</keyword>
    <feature type="domain" description="Copper amine oxidase N2-terminal" evidence="14">
        <location>
            <begin position="13"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase N3-terminal" evidence="15">
        <location>
            <begin position="105"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="13">
        <location>
            <begin position="230"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AAK41902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="660" mass="77137" checksum="413121DF91C74076" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XM9</accession>
    <name>Q97XM9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1173</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90329">
        <property type="entry name" value="G90329"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XM9"/>
    <dbReference type="STRING" id="273057.SSO8685"/>
    <dbReference type="PaxDb" id="273057-SSO8685"/>
    <dbReference type="EnsemblBacteria" id="AAK41894">
        <property type="protein sequence ID" value="AAK41894"/>
        <property type="gene ID" value="SSO8685"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8685"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1734"/>
    <dbReference type="eggNOG" id="arCOG02133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2968629_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XM9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="58" mass="6467" checksum="2B7229ED90BE733A" modified="2001-10-01" version="1">MRVTRMNLVTLAQLILMILRNLNLKPRKYELKDIALALAAYSLGVQITKTGIPPSTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XN9</accession>
    <name>Q97XN9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1674</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90328">
        <property type="entry name" value="E90328"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989131.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XN9"/>
    <dbReference type="STRING" id="273057.SSO1674"/>
    <dbReference type="PaxDb" id="273057-SSO1674"/>
    <dbReference type="EnsemblBacteria" id="AAK41884">
        <property type="protein sequence ID" value="AAK41884"/>
        <property type="gene ID" value="SSO1674"/>
    </dbReference>
    <dbReference type="GeneID" id="1454663"/>
    <dbReference type="KEGG" id="sso:SSO1674"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1721"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123170_2_2_2"/>
    <dbReference type="InParanoid" id="Q97XN9"/>
    <dbReference type="PhylomeDB" id="Q97XN9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="7"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="128" mass="14585" checksum="DF0B9D572CBE0168" modified="2001-10-01" version="1">MRYTDWMIQAEEDLDVARVLLEAGKYFAVTFYSQQAVEKALKSLLIHLGKDPGKTHSLTELASMIENEGLKIPQNIMEDLMVISPHFIISRYPDAANGVPARQYTKNMAEDLYRRAKEVLGWVKGNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XP0</accession>
    <name>Q97XP0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2007-09" db="PDB data bank">
            <title>Crystal structure of a putative nucleotidyltransferase (NP_343093.1) from Sulfolobus solfataricus at 1.40 A resolution.</title>
            <authorList>
                <consortium name="Joint Center for Structural Genomics (JCSG)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90328">
        <property type="entry name" value="D90328"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989132.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2RFF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.40 A"/>
        <property type="chains" value="A=1-110"/>
    </dbReference>
    <dbReference type="PDBsum" id="2RFF"/>
    <dbReference type="AlphaFoldDB" id="Q97XP0"/>
    <dbReference type="SMR" id="Q97XP0"/>
    <dbReference type="STRING" id="273057.SSO1673"/>
    <dbReference type="PaxDb" id="273057-SSO1673"/>
    <dbReference type="DNASU" id="1454662"/>
    <dbReference type="EnsemblBacteria" id="AAK41883">
        <property type="protein sequence ID" value="AAK41883"/>
        <property type="gene ID" value="SSO1673"/>
    </dbReference>
    <dbReference type="GeneID" id="1454662"/>
    <dbReference type="KEGG" id="sso:SSO1673"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1720"/>
    <dbReference type="eggNOG" id="arCOG01197">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130257_9_4_2"/>
    <dbReference type="InParanoid" id="Q97XP0"/>
    <dbReference type="PhylomeDB" id="Q97XP0"/>
    <dbReference type="EvolutionaryTrace" id="Q97XP0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052548">
        <property type="entry name" value="Type_VII_TA_antitoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933:SF1">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE MNTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2RFF"/>
        </source>
    </evidence>
    <sequence length="110" mass="12823" checksum="C53E86D666E9451D" modified="2001-10-01" version="1">MGKGKSAIESQIRMLKLAKEIVEEVASSFPNLEEVYIFGSRARGDYLDTSDIDILFVFKGIKEMNVFDRMYMVSRFIRGNVDYIVLDEGEKDRVKDKVLFWKREKGFVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XX4</accession>
    <name>Q97XX4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1586</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90317">
        <property type="entry name" value="D90317"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923485.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XX4"/>
    <dbReference type="PaxDb" id="273057-SSO1586"/>
    <dbReference type="EnsemblBacteria" id="AAK41795">
        <property type="protein sequence ID" value="AAK41795"/>
        <property type="gene ID" value="SSO1586"/>
    </dbReference>
    <dbReference type="GeneID" id="7806185"/>
    <dbReference type="KEGG" id="sso:SSO1586"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1620"/>
    <dbReference type="eggNOG" id="arCOG03861">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1056018_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XX4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="283" mass="31986" checksum="D2135004E526CF97" modified="2001-10-01" version="1">MYSTELTLNSPIWIVRKFLMDPLFISGVSGHISILKFKDKQKDEYIMGDRIRELSAPTDEFIALYILLRTNKDFKYTEGIFNGPKVTMQSIKYSGMSDDGKLEFTIEFMPKSLGDTQTRLYVATNVKYNDSLLDKMLGKSAVDFAKHIVEDHFATYARVYFPSLYGDIIRSLTNKETDKEKTTQGLSLTPLFEFSGDATSILSKINEMVSQLDLGLIKVNFGKVNCNIIVQNKDMKRAICRSDSNVKAGFEALSLIISAKGQGKMEVYSVKMEDLIDTLYTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XZ6</accession>
    <name>Q97XZ6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1562</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90314">
        <property type="entry name" value="H90314"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XZ6"/>
    <dbReference type="STRING" id="273057.SSO1562"/>
    <dbReference type="PaxDb" id="273057-SSO1562"/>
    <dbReference type="EnsemblBacteria" id="AAK41775">
        <property type="protein sequence ID" value="AAK41775"/>
        <property type="gene ID" value="SSO1562"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1562"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1595"/>
    <dbReference type="eggNOG" id="arCOG03167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_968456_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XZ6"/>
    <dbReference type="PhylomeDB" id="Q97XZ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295:SF18">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="46"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="287" mass="33717" checksum="4EA10BD8E9DF7E69" modified="2001-10-01" version="1">MTLTIVVSYISQMSFQNPWWVDKNRILEDEHVKRARVIVPPIDESALILGPRQVGKTTYLKTTIKTLLERVDSRSILFFSCDAFSKKEELIGLVNEYRTLVNREESAYIFLDEITSVSDWNTALLHLFNAGYFNNSLVYVTGSSSINLRKEMLPGRPIKKYVFYPLNFRVYFDSFYKKLIQDHVKVDEKDELYENALKLLPYISELNKALLEYVRRGGFFATSFSENPLSLYETYKDAILSEFLKTERKESVFKVIIRKIIESYGSRISENTSAFRKFMAYRQRIYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y03</accession>
    <name>Q97Y03_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1553</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99314">
        <property type="entry name" value="A99314"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y03"/>
    <dbReference type="PaxDb" id="273057-SSO1553"/>
    <dbReference type="EnsemblBacteria" id="AAK41768">
        <property type="protein sequence ID" value="AAK41768"/>
        <property type="gene ID" value="SSO1553"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1553"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1589"/>
    <dbReference type="HOGENOM" id="CLU_1536733_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y03"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="178" mass="20356" checksum="203D67529A5A5152" modified="2001-10-01" version="1">MLIEMKVMKIAVPRMDFRHLSLLKANPIKLKISIYIYYIVNSKEKLIKYLRDHGGKIARSKVKWASEELLNELEKEGIISVIRRSNGSSIQLLDKGIQERNSTNSAQQSEEEKGKSDSSEITSLLKEINKKLDIILQKLGQSIEIDFDKVFEEVKNPMGIASLKDIREGRSNERGILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y25</accession>
    <name>Q97Y25_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Clan AA aspartic protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1523</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90311">
        <property type="entry name" value="C90311"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y25"/>
    <dbReference type="STRING" id="273057.SSO1523"/>
    <dbReference type="PaxDb" id="273057-SSO1523"/>
    <dbReference type="EnsemblBacteria" id="AAK41746">
        <property type="protein sequence ID" value="AAK41746"/>
        <property type="gene ID" value="SSO1523"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1523"/>
    <dbReference type="eggNOG" id="arCOG04029">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161799_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y25"/>
    <dbReference type="PhylomeDB" id="Q97Y25"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022274">
        <property type="entry name" value="Peptidase_asp_AF0612"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03698">
        <property type="entry name" value="clan_AA_DTGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14667" checksum="0B61674A4CB540EF" modified="2001-10-01" version="1">MERVNSLECFDINEKPLLHVKVSDFKNENSVEIDALIDTGFSGWLLLNYEIYTKLNSLEIPITRKYRSILGNIEVFMSKASITINRLNIDAFIESSPYIEMNLLGREILKKLNICFYRMNKVCILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y36</accession>
    <name>Q97Y36_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR type III-associated protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1514</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90309">
        <property type="entry name" value="H90309"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990106.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y36"/>
    <dbReference type="SMR" id="Q97Y36"/>
    <dbReference type="STRING" id="273057.SSO1514"/>
    <dbReference type="PaxDb" id="273057-SSO1514"/>
    <dbReference type="EnsemblBacteria" id="AAK41735">
        <property type="protein sequence ID" value="AAK41735"/>
        <property type="gene ID" value="SSO1514"/>
    </dbReference>
    <dbReference type="GeneID" id="1454514"/>
    <dbReference type="KEGG" id="sso:SSO1514"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1553"/>
    <dbReference type="eggNOG" id="arCOG02657">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047795_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y36"/>
    <dbReference type="PhylomeDB" id="Q97Y36"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09682">
        <property type="entry name" value="Cmr4_III-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013410">
        <property type="entry name" value="CRISPR-assoc_RAMP_Cmr4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02580">
        <property type="entry name" value="cas_RAMP_Cmr4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36700">
        <property type="entry name" value="CRISPR SYSTEM CMR SUBUNIT CMR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36700:SF1">
        <property type="entry name" value="CRISPR SYSTEM CMR SUBUNIT CMR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="2">
        <location>
            <begin position="11"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="269" mass="29872" checksum="EA97E1EC79813C94" modified="2001-10-01" version="1">MVPKYLVLAYAITPIHVGAGKSSTGVVDSPLVRDSIGYPIVYGSSLKGSLKSFLIAKNESLAKCVFGGKPEEENILTSKYVLTDLIPVFYPVSSIDEGYIYITTRYLINHIEDLMSRLGIGSLYKESKEKSEVRIFLGKISTELSLQLSDEVRSLGNLIKDKNRVYVLDNSIGLSAVESALTRVTRTELDDNTKTSKNIWSEEYIPQGTILLSGIFEREISNSYCEELNRNNVNIDNEFLKLIDNVSVFIGGKESIGKGLTKIKLKRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y47</accession>
    <name>Q97Y47_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90308">
        <property type="entry name" value="E90308"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y47"/>
    <dbReference type="PaxDb" id="273057-SSO1500"/>
    <dbReference type="EnsemblBacteria" id="AAK41724">
        <property type="protein sequence ID" value="AAK41724"/>
        <property type="gene ID" value="SSO1500"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1500"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1542"/>
    <dbReference type="eggNOG" id="arCOG05901">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_311621_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y47"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="1039" mass="121123" checksum="2012C2AC9236A750" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y81</accession>
    <name>Q97Y81_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gdhA-1</name>
        <name evidence="10" type="ordered locus">SSO1457</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
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            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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    </reference>
    <reference evidence="12 13" key="2">
        <citation type="journal article" date="2025" name="Extremophiles" volume="29" first="32" last="32">
            <title>Structural insight into the unique substrate specificity of glutamate dehydrogenase from Saccharolobus solfataricus.</title>
            <authorList>
                <person name="Okabe I."/>
                <person name="Hirano M."/>
                <person name="Ohmori T."/>
                <person name="Segawa M."/>
                <person name="Yoneda K."/>
                <person name="Ohshima T."/>
                <person name="Sakuraba H."/>
            </authorList>
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            <dbReference type="DOI" id="10.1007/s00792-025-01395-1"/>
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        <scope>X-RAY CRYSTALLOGRAPHY (1.73 ANGSTROMS) IN COMPLEX WITH 2-OXOGLUTARATE AND NAD(+)</scope>
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        <text evidence="2">Homohexamer.</text>
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        <text evidence="1 4 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
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                <person name="Confalonieri F."/>
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                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
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                <person name="Medina N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054712">
        <property type="entry name" value="Cas3-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006474">
        <property type="entry name" value="Helicase_Cas3_CRISPR-ass_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01587">
        <property type="entry name" value="cas3_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF16">
        <property type="entry name" value="CRISPR-ASSOCIATED NUCLEASE_HELICASE CAS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22590">
        <property type="entry name" value="Cas3-like_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="41"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="245"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="515" mass="57946" checksum="899705D98EEA75E4" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YJ7</accession>
    <name>Q97YJ7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NurA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90288">
        <property type="entry name" value="C90288"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923319.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YJ7"/>
    <dbReference type="PaxDb" id="273057-SSO1325"/>
    <dbReference type="DNASU" id="1454341"/>
    <dbReference type="EnsemblBacteria" id="AAK41562">
        <property type="protein sequence ID" value="AAK41562"/>
        <property type="gene ID" value="SSO1325"/>
    </dbReference>
    <dbReference type="GeneID" id="1454341"/>
    <dbReference type="KEGG" id="sso:SSO1325"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1326"/>
    <dbReference type="eggNOG" id="arCOG00287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_670176_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YJ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018977">
        <property type="entry name" value="NurA_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09376">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00933">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NurA" evidence="1">
        <location>
            <begin position="65"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="390" mass="45736" checksum="925C3071091C98A3" modified="2001-10-01" version="1">MNWKVTIKNTIEELAKLKKDVKIENEGDPEHELSDAEDKVMKIELEEEFKPTMYSLYEFKSLNDKRIASIDSSSRYLRDFSVNTCLIGLSVYSNREGFIDGPFTVDIPYIGISSYREILESLKIDDINVRYKNVVNYYYVNEPNNEYKIDDIADELRLEAENVGLKRVIQNHDIVIIDGPIYPTPLELTEEINLETEARRKHRLAYAKLVNERIELLNNNVIGVVKRLENSKKLWRVKEINETIGNVKRMKDVTIIELIDERLCRKNYEYVCTIGPFKIEYALEVKDEDGRNVIEKVPTKYAYYVIIRKKYFPPTFLRIESTNKNLDISPVLSRLTERLMPTYIEIVDRRSKRISASLFLYAYEIASNYLNIIHDDKMGYLSVISQFTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YP6</accession>
    <name>Q97YP6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1278</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90282">
        <property type="entry name" value="D90282"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990042.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YP6"/>
    <dbReference type="STRING" id="273057.SSO1278"/>
    <dbReference type="PaxDb" id="273057-SSO1278"/>
    <dbReference type="EnsemblBacteria" id="AAK41515">
        <property type="protein sequence ID" value="AAK41515"/>
        <property type="gene ID" value="SSO1278"/>
    </dbReference>
    <dbReference type="GeneID" id="1454294"/>
    <dbReference type="KEGG" id="sso:SSO1278"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1276"/>
    <dbReference type="HOGENOM" id="CLU_1149854_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YP6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="241" mass="27066" checksum="C80A3A3246E10EFF" modified="2001-10-01" version="1">MLHATYINLFLSLFFFFPLFLSLTFYGTQLIGYHGVLSSLAINMNVGVSVNHLPDGGYNVTIREIGNSYNKTLGIQLSYNGEYYYIINMSGKEIWITYYISNASSNIGLLAPFYMPYSTNGTYSFNEKIVNMSNNKIESQIITNISIIKQNSGKLVVNFSDLNITTIPTYYVSQRIIYVNGLISQMQSNYTNSGQIYLYTNISRMEGGTDYSGVIIPSVVIAIIIIVASSIAVYRFRKSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YV4</accession>
    <name>Q97YV4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Partial transposase ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1198</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90273">
        <property type="entry name" value="H90273"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YV4"/>
    <dbReference type="STRING" id="273057.SSO1198"/>
    <dbReference type="PaxDb" id="273057-SSO1198"/>
    <dbReference type="EnsemblBacteria" id="AAK41447">
        <property type="protein sequence ID" value="AAK41447"/>
        <property type="gene ID" value="SSO1198"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1198"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1198"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113598_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YV4"/>
    <dbReference type="PhylomeDB" id="Q97YV4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="210" mass="24216" checksum="380386CE84C0FF81" modified="2001-10-01" version="1">MEAPVAGIDVSKDKLVVYFQGKYYEFQNNKQGFEGVKQVLPKGCKVAIESTGIYHINLAKYLGKEYDVRIINPLVLKKFKDIRGKKSDKNDAKKLAELVISMGSEFTTSEAKESTSQWDFITRSIVRVKNRLRRDLILLGYKDSLSKKNLNEVLRGEDNIVLSEVRFLLDELERLKVRKREIEEELEDLVPKDSLIFTSISGKFLDFNFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YY9</accession>
    <name>Q97YY9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90269">
        <property type="entry name" value="D90269"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991583.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YY9"/>
    <dbReference type="SMR" id="Q97YY9"/>
    <dbReference type="STRING" id="273057.SSO1161"/>
    <dbReference type="PaxDb" id="273057-SSO1161"/>
    <dbReference type="EnsemblBacteria" id="AAK41411">
        <property type="protein sequence ID" value="AAK41411"/>
        <property type="gene ID" value="SSO1161"/>
    </dbReference>
    <dbReference type="GeneID" id="1454190"/>
    <dbReference type="KEGG" id="sso:SSO1161"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1154"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1811487_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YY9"/>
    <dbReference type="PhylomeDB" id="Q97YY9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="14"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="142" mass="16443" checksum="CE1F2118F21D4B27" modified="2001-10-01" version="1">MIPPKFREYSKAFLNIAKKDNIRAKRALELKDYPECLFYSQQSVEKSVKAMLEVKLIYKKEHDIIADASNNLQDLGNELDVVLNALDYLSGAWNISRYPFFNGNNITTPEEFVTEEMCKEGIKYSDEVIEIAENYLRKHGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZA0</accession>
    <name>Q97ZA0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3-oxoacyl-(Acyl carrier protein) reductase (FabG-2)</fullName>
            <ecNumber evidence="4">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fabG-2</name>
        <name evidence="4" type="ordered locus">SSO1030</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90254">
        <property type="entry name" value="F90254"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989207.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZA0"/>
    <dbReference type="FunCoup" id="Q97ZA0">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1030"/>
    <dbReference type="PaxDb" id="273057-SSO1030"/>
    <dbReference type="EnsemblBacteria" id="AAK41293">
        <property type="protein sequence ID" value="AAK41293"/>
        <property type="gene ID" value="SSO1030"/>
    </dbReference>
    <dbReference type="GeneID" id="84058576"/>
    <dbReference type="KEGG" id="sso:SSO1030"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1022"/>
    <dbReference type="eggNOG" id="arCOG01263">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_1_2"/>
    <dbReference type="InParanoid" id="Q97ZA0"/>
    <dbReference type="PhylomeDB" id="Q97ZA0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000819">
        <property type="entry name" value="Short-chain dehydrogenase/reductase SDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169:SF6">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="247" mass="27394" checksum="17B72C6E014FF650" modified="2001-10-01" version="1">MSEVAIVTGASKGIGRAVVKLLKENNYTVVSISRSKSDIGDVIYDADVSDRSTVFRIVNEVLEKFGKIDVLVNNAGFGVYGSFLETDLNEEEYMIRTNLLAPLYFMKAVLPHMVSRRKGSVVNIVSEAAYVSTPKLLVYSATKAGLASLTNGLWAEMRKYNVRVSGVYPGPVRTNFTSHPSFKKNNGDPFANYSVEPEAVAKAVLKAIRTGKREIYVPSRLKLDPYFLKLANLFQSFTYTIVSNYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZF2</accession>
    <name>Q97ZF2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 15">tRNA-splicing ligase RtcB</fullName>
            <ecNumber evidence="15">6.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">rtcB</name>
        <name evidence="16" type="ordered locus">SSO0966</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential for tRNA splicing and maturation. Acts by directly joining spliced tRNA halves to mature-sized tRNAs. Joins RNA with 2',3'-cyclic-phosphate or 3'-phosphate ends to RNA with 5'-hydroxy ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68080"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:68076"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 15">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="14 15">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 15">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 15">Belongs to the RtcB family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.-" evidence="15"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90248">
        <property type="entry name" value="A90248"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992413.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZF2"/>
    <dbReference type="FunCoup" id="Q97ZF2">
        <property type="interactions" value="230"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0966"/>
    <dbReference type="PaxDb" id="273057-SSO0966"/>
    <dbReference type="EnsemblBacteria" id="AAK41240">
        <property type="protein sequence ID" value="AAK41240"/>
        <property type="gene ID" value="SSO0966"/>
    </dbReference>
    <dbReference type="GeneID" id="1455207"/>
    <dbReference type="KEGG" id="sso:SSO0966"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.965"/>
    <dbReference type="eggNOG" id="arCOG04246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022279_0_1_2"/>
    <dbReference type="InParanoid" id="Q97ZF2"/>
    <dbReference type="PhylomeDB" id="Q97ZF2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170057">
        <property type="term" value="F:RNA ligase (GTP) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1860.10:FF:000001">
        <property type="entry name" value="tRNA-splicing ligase RtcB homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="entry name" value="tRNA-splicing ligase RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118:SF1">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="Hypothetical protein PH1602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 14 15" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="GMP-histidine intermediate" evidence="12">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="204"/>
            <end position="208"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="331"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="380"/>
            <end position="383"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="405"/>
            <end position="408"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="481"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU371113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AAK41240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="482" mass="53005" checksum="9158245590E20F12" modified="2001-10-01" version="1">MQINITRVSTYEWRIDKGAQECMKVPVTIFADDVLIEKMKQDMTLRQATNVACLPGVQESIYVLPDGHQGYGFPIGGIAATAIEEGGVVSPGGIGYDINCGVRLLRTNLDYKDVKPKLAQLVEELHRNVPSGVGSEGKVKLTYQQLDQVLAEGVAWAVDKGFGWKEDMNHMEQRGSWELADPSKVSPIAKQRGASQLGTLGAGNHFLEIQVVDKIFDPQIAKAIGVDHEGQVMVMVHTGSRGLGHQVASDYLQIMERAMKKYNIQLPDRELAAVPFESREGQDYFHAMASGANFAWTNRQLITHWTRESFGRVFGVDPEKLDLSIVYDVAHNIAKIEEYVIGGERKKVLVHRKGATRAFPPGSPEIPADHRNIGQIVLIPGSMGTASYVMAGIPEGRRTWFTAPHGAGRWMSREAAVRNYPANVVVETLAEKGIVVRAATRRVVAEEAPGAYKDVDRVAKVAHEVKIAKLVMRLRPIGVTKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZH7</accession>
    <name>Q97ZH7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0934</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90244">
        <property type="entry name" value="D90244"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992366.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZH7"/>
    <dbReference type="STRING" id="273057.SSO0934"/>
    <dbReference type="PaxDb" id="273057-SSO0934"/>
    <dbReference type="EnsemblBacteria" id="AAK41211">
        <property type="protein sequence ID" value="AAK41211"/>
        <property type="gene ID" value="SSO0934"/>
    </dbReference>
    <dbReference type="GeneID" id="1455178"/>
    <dbReference type="KEGG" id="sso:SSO0934"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.931"/>
    <dbReference type="eggNOG" id="arCOG05980">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_848917_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZH7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="346" mass="40220" checksum="06486EEC01304578" modified="2001-10-01" version="1">MKLDDIIKVAAEYPFKTLSENIELQDDMLSIEQLPQLLTIGGVKRVKWKYKAKILGPDLSTILTEGTENEEELIIRTPLHKVSIPWIFTRLDTDSLKKLVEYLIPCKEGISLFNISPWPRYYFMQNRIIELKEGEIGNGRNVSLENIKLTENQISINTRFVNPKFFYMNPYYIESSYNPIRNTFAASLELTEAYSFVSNSLMDLEFELGKISVEANGKILVSKTRTFTESKLHRLLWDMTNDVIEIECNPQFPLSLYRIEPSSIIPLYMKFDEKTNILQIVLENFSDKPVIATVYISARITKILKPNNTLTTEYDRVKIPIRRWGIINLELEIKKLPDLLLKRKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZH8</accession>
    <name>Q97ZH8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0931</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90244">
        <property type="entry name" value="C90244"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992364.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZH8"/>
    <dbReference type="STRING" id="273057.SSO0931"/>
    <dbReference type="PaxDb" id="273057-SSO0931"/>
    <dbReference type="DNASU" id="1455177"/>
    <dbReference type="EnsemblBacteria" id="AAK41210">
        <property type="protein sequence ID" value="AAK41210"/>
        <property type="gene ID" value="SSO0931"/>
    </dbReference>
    <dbReference type="GeneID" id="1455177"/>
    <dbReference type="KEGG" id="sso:SSO0931"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.930"/>
    <dbReference type="eggNOG" id="arCOG00017">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133047_2_1_2"/>
    <dbReference type="InParanoid" id="Q97ZH8"/>
    <dbReference type="PhylomeDB" id="Q97ZH8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40730:SF3">
        <property type="entry name" value="HTH CRO_C1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40730">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13785" checksum="653D60102B63FCD7" modified="2001-10-01" version="1">MSLQLPCEFSVREILPAVRSIVAQKLIKERNLSEYKAANLMGLTPAAVSNYLKSRRGSNLRSLLEKDEKFMDLVNEVTERILNSNSNLSVYYCILCSEGKKVLTKHGYALSPCLYETIVEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZM0</accession>
    <name>Q97ZM0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0872</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA damage repair.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nre family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99238">
        <property type="entry name" value="A99238"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992291.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZM0"/>
    <dbReference type="STRING" id="273057.SSO0872"/>
    <dbReference type="PaxDb" id="273057-SSO0872"/>
    <dbReference type="EnsemblBacteria" id="AAK41160">
        <property type="protein sequence ID" value="AAK41160"/>
        <property type="gene ID" value="SSO0872"/>
    </dbReference>
    <dbReference type="GeneID" id="1455127"/>
    <dbReference type="KEGG" id="sso:SSO0872"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.881"/>
    <dbReference type="eggNOG" id="arCOG04269">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039703_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZM0"/>
    <dbReference type="PhylomeDB" id="Q97ZM0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02096">
        <property type="entry name" value="Nre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033167">
        <property type="entry name" value="Nre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006979">
        <property type="entry name" value="Nre_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006978">
        <property type="entry name" value="Nre_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38136">
        <property type="entry name" value="DNA REPAIR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38136:SF3">
        <property type="entry name" value="DNA REPAIR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04895">
        <property type="entry name" value="Nre_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04894">
        <property type="entry name" value="Nre_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Archaeal Nre N-terminal" evidence="2">
        <location>
            <begin position="18"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Archaeal Nre C-terminal" evidence="3">
        <location>
            <begin position="303"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="408" mass="47049" checksum="D47679548A3BFC41" modified="2001-10-01" version="1">MHKIPAELCIKCKSHKNLCGLPYCPIMERFRGMVSSLQKIEIDTSFKLVEGSTPPSGIVGEKGYPKVSLIVNIPPSVYGEDARKYENVKEWWGRINLGDIIKLRSSLISSITTVKVEKATEYYDTEIPLAIISDNPIVTEAKLKTLEAKLKFDGVILPRGPSGIAEQIKVLDNPKVPTKLDKLIFDDAKSTEAILELYRYNVDYYKIIHALSFGLLGRKKNRRFVPTRWAITAVDSIVGKFLYNKIINYNEINEIEVYHSSYLGNYFYVILYPSKFNSTWIEIWHPLSLWSQELTISELKENFWGEYEYLDGGYMAARLAVLEHLEQIKRQSGIIIIREITEEYFAPVGNWHIRETVRNAMKNRLGKYDNLNLAISEVNKKLKAKINLFELRTINSLINQKSIYDFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZP8</accession>
    <name>Q97ZP8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CopG family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO6469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90225">
        <property type="entry name" value="C90225"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991348.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZP8"/>
    <dbReference type="PaxDb" id="273057-SSO6469"/>
    <dbReference type="EnsemblBacteria" id="AAK41058">
        <property type="protein sequence ID" value="AAK41058"/>
        <property type="gene ID" value="SSO6469"/>
    </dbReference>
    <dbReference type="GeneID" id="1455025"/>
    <dbReference type="KEGG" id="sso:SSO6469"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.759"/>
    <dbReference type="eggNOG" id="arCOG08305">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2662403_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZP8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="43"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="9052" checksum="9B19101BE280DE9A" modified="2001-10-01" version="1">MPRPGYKSVYFPDEELWKKIVDEAEKRKVSVYEVLKDAFNCYIREKEGNKVSMEEIVKELQELKKRVEELEKKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZT7</accession>
    <name>Q97ZT7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphate transport system permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pstC</name>
        <name evidence="12" type="ordered locus">SSO0490</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90194">
        <property type="entry name" value="D90194"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923005.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZT7"/>
    <dbReference type="FunCoup" id="Q97ZT7">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0490"/>
    <dbReference type="PaxDb" id="273057-SSO0490"/>
    <dbReference type="EnsemblBacteria" id="AAK40811">
        <property type="protein sequence ID" value="AAK40811"/>
        <property type="gene ID" value="SSO0490"/>
    </dbReference>
    <dbReference type="GeneID" id="1455621"/>
    <dbReference type="GeneID" id="27426787"/>
    <dbReference type="KEGG" id="sso:SSO0490"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.484"/>
    <dbReference type="eggNOG" id="arCOG00167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_1_3_2"/>
    <dbReference type="InParanoid" id="Q97ZT7"/>
    <dbReference type="PhylomeDB" id="Q97ZT7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011864">
        <property type="entry name" value="Phosphate_PstC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051124">
        <property type="entry name" value="Phosphate_Transport_Permease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02138">
        <property type="entry name" value="phosphate_pstC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30425">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN PST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30425:SF1">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN PSTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="67"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK40811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="294" mass="32514" checksum="8F103490E403A5BB" modified="2001-10-01" version="1">MITIKRKREKFFYFLLIPSSVVVFTFILILSVLVYNSIPILEKVGLSIYTKNIWNPIYDQYGGLAAIYGTIVTSVLSVMISIPFATAFSIFVNDISPKKVKSFLVNLSDLLAAFPTVLYGFWGLLSLGPFLSRTLFTFLSSHVGLHQTTNGPSILLASIILAIMITPFASSLIREVYFQVPRSFEEAVYSLGLGKWELITLKLSYIKKAFLGAYALAFGRAVGETVAVSLTIGNVLNISPNLFEPGYTISSLIISQFGTAYGIQYNAMFALALFLVVIGFIFVIISKLIVRMRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2021-09-29" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q980B8</accession>
    <name>RPO13_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit Rpo13</fullName>
            <ecNumber evidence="2">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpo13</name>
        <name type="ordered locus">SSO0396</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Nature" volume="451" first="851" last="854">
            <title>The X-ray crystal structure of RNA polymerase from Archaea.</title>
            <authorList>
                <person name="Hirata A."/>
                <person name="Klein B.J."/>
                <person name="Murakami K.S."/>
            </authorList>
            <dbReference type="PubMed" id="18235446"/>
            <dbReference type="DOI" id="10.1038/nature06530"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.40 ANGSTROMS) OF THE RNA POLYMERASE COMPLEX</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Probably binds dsDNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 8">Part of the 13-subunit RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo13 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90183">
        <property type="entry name" value="F90183"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988796.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3HKZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="Y/Z=1-104"/>
    </dbReference>
    <dbReference type="PDBsum" id="3HKZ"/>
    <dbReference type="AlphaFoldDB" id="Q980B8"/>
    <dbReference type="SMR" id="Q980B8"/>
    <dbReference type="STRING" id="273057.SSO0396"/>
    <dbReference type="PaxDb" id="273057-SSO0396"/>
    <dbReference type="EnsemblBacteria" id="AAK40725">
        <property type="protein sequence ID" value="AAK40725"/>
        <property type="gene ID" value="SSO0396"/>
    </dbReference>
    <dbReference type="GeneID" id="1455535"/>
    <dbReference type="KEGG" id="sso:SSO0396"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.392"/>
    <dbReference type="eggNOG" id="arCOG05938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177471_0_0_2"/>
    <dbReference type="InParanoid" id="Q980B8"/>
    <dbReference type="BRENDA" id="2.7.7.6">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q980B8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021985">
        <property type="entry name" value="RNA_pol_Rpo13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12136">
        <property type="entry name" value="RNA_pol_Rpo13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000453789" description="DNA-directed RNA polymerase subunit Rpo13">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="60"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="turn" evidence="9">
        <location>
            <begin position="73"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="B8YB65"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q4JAJ6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="18235446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="3HKZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="3HKZ"/>
        </source>
    </evidence>
    <sequence length="104" mass="12176" checksum="298E559164C310EE" modified="2001-10-01" version="1">MVSGMSTDEEKEGTSDEEVNEEKEVEETSEDEFPKLSIQDIELLMRNTEIWDNLLNGKITLEEAKKLFEDNYKEYEKRDSRRKAKKAVSKKVKKTKKKEKSVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q980B9</accession>
    <name>Q980B9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO0395</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 9">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HerA family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90183">
        <property type="entry name" value="E90183"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988797.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980B9"/>
    <dbReference type="STRING" id="273057.SSO0395"/>
    <dbReference type="PaxDb" id="273057-SSO0395"/>
    <dbReference type="EnsemblBacteria" id="AAK40724">
        <property type="protein sequence ID" value="AAK40724"/>
        <property type="gene ID" value="SSO0395"/>
    </dbReference>
    <dbReference type="GeneID" id="1455534"/>
    <dbReference type="KEGG" id="sso:SSO0395"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.391"/>
    <dbReference type="eggNOG" id="arCOG00285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036272_0_0_2"/>
    <dbReference type="InParanoid" id="Q980B9"/>
    <dbReference type="PhylomeDB" id="Q980B9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF1">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="250"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="572" mass="64565" checksum="87D7683C7281AE99" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q980F4</accession>
    <name>Q980F4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF711 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0358</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99179">
        <property type="entry name" value="A99179"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922951.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980F4"/>
    <dbReference type="STRING" id="273057.SSO0358"/>
    <dbReference type="PaxDb" id="273057-SSO0358"/>
    <dbReference type="EnsemblBacteria" id="AAK40688">
        <property type="protein sequence ID" value="AAK40688"/>
        <property type="gene ID" value="SSO0358"/>
    </dbReference>
    <dbReference type="GeneID" id="1455498"/>
    <dbReference type="KEGG" id="sso:SSO0358"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.349"/>
    <dbReference type="eggNOG" id="arCOG04321">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064235_0_0_2"/>
    <dbReference type="InParanoid" id="Q980F4"/>
    <dbReference type="PhylomeDB" id="Q980F4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014537">
        <property type="entry name" value="UCP027893"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007841">
        <property type="entry name" value="UPF0210"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37560:SF2">
        <property type="entry name" value="DUF711 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37560">
        <property type="entry name" value="UPF0210 PROTEIN SPR0218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05167">
        <property type="entry name" value="DUF711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027893">
        <property type="entry name" value="UCP027893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="37795" checksum="2CE5C384909C8EEE" modified="2001-10-01" version="1">MMIRAITIFVTNYSKISDYTEKLSKLNDNNIWSKRISLPPTPIDLSLSKLIELLPTTHSEIIFSLINLQEKDKRLREIKDILKSDKRIYVHVLVKTTDNLKDLAKLIISLEPEEASRFALLFNQDFLLTPYFPTSSANTVYDSFGISLLYVDEFKQGKINEALSKADTIGRQLEKKLNIKYLGIDASLSPWMESSVGEIVESRSKKMFDISNLSAVAEINREIFEGVWRNRVNPIGFSELMLPIAEDNLLRERVKEGSLTLKDLLLMSYVCVAGIDMVGIYSDLVTYENILKSVYYIQYTKRKPYGVRMIPTNGSPVLTKMFGEIPEVKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q980G2</accession>
    <name>Q980G2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF434 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90176">
        <property type="entry name" value="G90176"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990628.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980G2"/>
    <dbReference type="STRING" id="273057.SSO0340"/>
    <dbReference type="PaxDb" id="273057-SSO0340"/>
    <dbReference type="EnsemblBacteria" id="AAK40670">
        <property type="protein sequence ID" value="AAK40670"/>
        <property type="gene ID" value="SSO0340"/>
    </dbReference>
    <dbReference type="GeneID" id="1455480"/>
    <dbReference type="KEGG" id="sso:SSO0340"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.331"/>
    <dbReference type="eggNOG" id="arCOG03229">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102155_1_0_2"/>
    <dbReference type="InParanoid" id="Q980G2"/>
    <dbReference type="PhylomeDB" id="Q980G2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007368">
        <property type="entry name" value="DUF434"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041652">
        <property type="entry name" value="DUF5616"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42252:SF1">
        <property type="entry name" value="DUF434 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42252">
        <property type="entry name" value="DUF5616 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04256">
        <property type="entry name" value="DUF434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18481">
        <property type="entry name" value="DUF5616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF434" evidence="1">
        <location>
            <begin position="7"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5616" evidence="2">
        <location>
            <begin position="65"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="24818" checksum="878E5D76FCC8E11C" modified="2001-10-01" version="1">MYFTDVFREAYLDYKYFLNRDYSRKVVLDIIAAKYNLSSLERLLLYRCVHSDKEIEIIKGKINNEEREVVLDGYNLALTLISAINNDELYLCDDGFLRDLGLGRHKSSEIISDTLILISEYCEKYRIKCEIILDKQLSKSGEITSKLRKKGIEIRTVNKADKEIIISNKAVYSNDFVILFKSQKISTILNTIFFESTFKILKGPWIANLEEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q980S3</accession>
    <name>Q980S3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2153 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90161">
        <property type="entry name" value="G90161"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990449.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980S3"/>
    <dbReference type="STRING" id="273057.SSO0205"/>
    <dbReference type="PaxDb" id="273057-SSO0205"/>
    <dbReference type="EnsemblBacteria" id="AAK40550">
        <property type="protein sequence ID" value="AAK40550"/>
        <property type="gene ID" value="SSO0205"/>
    </dbReference>
    <dbReference type="GeneID" id="1455360"/>
    <dbReference type="KEGG" id="sso:SSO0205"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.204"/>
    <dbReference type="eggNOG" id="arCOG04123">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142667_0_0_2"/>
    <dbReference type="InParanoid" id="Q980S3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014450">
        <property type="entry name" value="UCP008210"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09921">
        <property type="entry name" value="DUF2153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008210">
        <property type="entry name" value="UCP008210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="16694" checksum="0EFC85831831F9B7" modified="2001-10-01" version="1">MESSFIGNLDEWIKLQKNLLTTLKDMEKKEKTEDMDRLDLILASRTAFQHMMRTLKAFDQWLQDPMVIKHMPREMLEDVKNTSWELLQRLLELDIRHTSQFREMISKMSKEGKLDPLIWTRPVTEEHPERERRGPLSTI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q980S6</accession>
    <name>PYRG_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">CTP synthase</fullName>
            <ecNumber evidence="1 2">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="1">CTP synthetase</shortName>
            <shortName evidence="1">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrG</name>
        <name type="ordered locus">SSO0201</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Acta Crystallogr. F Struct. Biol. Commun." volume="67" first="201" last="208">
            <title>Structure of the dimeric form of CTP synthase from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Lauritsen I."/>
                <person name="Willemoes M."/>
                <person name="Jensen K.F."/>
                <person name="Johansson E."/>
                <person name="Harris P."/>
            </authorList>
            <dbReference type="PubMed" id="21301086"/>
            <dbReference type="DOI" id="10.1107/S1744309110052334"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS)</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen (PubMed:21301086). Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>UTP + NH4(+) + ATP = CTP + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
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    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
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    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
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    <comment type="subunit">
        <text evidence="2">Homotetramer in the presence of ATP and UTP. The enzyme dissociates into homodimers in the absence of substrate nucleotides.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
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        <text evidence="1">Belongs to the CTP synthase family.</text>
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</entry>
<entry dataset="Swiss-Prot" created="2003-09-26" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q980V2</accession>
    <name>TRUD_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable tRNA pseudouridine synthase D</fullName>
            <ecNumber evidence="1">5.4.99.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(13) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truD</name>
        <name type="ordered locus">SSO0174</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(13) in tRNA = pseudouridine(13) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42540"/>
            <dbReference type="Rhea" id="RHEA-COMP:10105"/>
            <dbReference type="Rhea" id="RHEA-COMP:10106"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.27" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90158">
        <property type="entry name" value="A90158"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990399.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980V2"/>
    <dbReference type="SMR" id="Q980V2"/>
    <dbReference type="FunCoup" id="Q980V2">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0174"/>
    <dbReference type="PaxDb" id="273057-SSO0174"/>
    <dbReference type="EnsemblBacteria" id="AAK40520">
        <property type="protein sequence ID" value="AAK40520"/>
        <property type="gene ID" value="SSO0174"/>
    </dbReference>
    <dbReference type="GeneID" id="1453347"/>
    <dbReference type="GeneID" id="44129140"/>
    <dbReference type="KEGG" id="sso:SSO0174"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.170"/>
    <dbReference type="eggNOG" id="arCOG04252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005281_4_1_2"/>
    <dbReference type="InParanoid" id="Q980V2"/>
    <dbReference type="PhylomeDB" id="Q980V2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160150">
        <property type="term" value="F:tRNA pseudouridine(13) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3160:FF:000001">
        <property type="entry name" value="Probable tRNA pseudouridine synthase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1510.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01082">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020119">
        <property type="entry name" value="PsdUridine_synth_TruD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF21">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE PUS7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000152554" description="Probable tRNA pseudouridine synthase D">
        <location>
            <begin position="1"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="TRUD" evidence="1">
        <location>
            <begin position="160"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01082"/>
        </source>
    </evidence>
    <sequence length="377" mass="44738" checksum="D92C98109131D915" modified="2001-10-01" version="1">MTPHEIDIALGMEKYYYEDWNELDGTIERPNGFKVIEEIDFKPAQDWKGETKGKYAVFLLTKWGIDHFSAISEVQKVLHSKVNYIGIKDANAITSQLVYIPLNEKQELIEKYQSRSFILKFLGFSSKKLNHTGNIFEISLSISDFDIVIERIEQIKKNPYLPAFIGYQRFGTRRPITHLIGKYLLRRDWEKAFYLILTYPFLSESKETIDIRKLIMEGDFKEAVRSIPSKFKQEKLLLKNYMRFNSYYLALKSSFIPISLYLDAYQSYLFNLYLSRKLDEYKNLNDKVNLLIRIPIYFNNCDDVCKEIYLDEGIERNFFKLQEFKISLRDLVRKAFMNIRDLKVNEETKTISFVLERGMYATILLREILRGDPRKFT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q980W5</accession>
    <name>LYSK_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">[LysW]-lysine/[LysW]-ornithine hydrolase</fullName>
            <ecNumber evidence="1">3.5.1.130</ecNumber>
            <ecNumber evidence="1">3.5.1.132</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lysK</name>
        <name type="ordered locus">SSO0162</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="29537" last="29549">
            <title>The Sulfolobus solfataricus Lrp-like protein LysM regulates lysine biosynthesis in response to lysine availability.</title>
            <authorList>
                <person name="Brinkman A.B."/>
                <person name="Bell S.D."/>
                <person name="Lebbink R.J."/>
                <person name="de Vos W.M."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="12042311"/>
            <dbReference type="DOI" id="10.1074/jbc.M203528200"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the release of L-lysine from [LysW]-gamma-L-lysine and the release of L-ornithine from [LysW]-L-ornithine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O = [amino-group carrier protein]-C-terminal-L-glutamate + L-lysine</text>
            <dbReference type="Rhea" id="RHEA:48684"/>
            <dbReference type="Rhea" id="RHEA-COMP:9693"/>
            <dbReference type="Rhea" id="RHEA-COMP:9715"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:78525"/>
            <dbReference type="ChEBI" id="CHEBI:78526"/>
            <dbReference type="EC" id="3.5.1.130"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-ornithyl)-L-glutamate + H2O = [amino-group carrier protein]-C-terminal-L-glutamate + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:52676"/>
            <dbReference type="Rhea" id="RHEA-COMP:9693"/>
            <dbReference type="Rhea" id="RHEA-COMP:13328"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:78525"/>
            <dbReference type="ChEBI" id="CHEBI:136763"/>
            <dbReference type="EC" id="3.5.1.132"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) or Co(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 5/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family. LysK subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.130" evidence="1"/>
    <dbReference type="EC" id="3.5.1.132" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BK000545">
        <property type="protein sequence ID" value="DAA00055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90156">
        <property type="entry name" value="D90156"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990386.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980W5"/>
    <dbReference type="SMR" id="Q980W5"/>
    <dbReference type="FunCoup" id="Q980W5">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0162"/>
    <dbReference type="MEROPS" id="M20.975"/>
    <dbReference type="PaxDb" id="273057-SSO0162"/>
    <dbReference type="EnsemblBacteria" id="AAK40507">
        <property type="protein sequence ID" value="AAK40507"/>
        <property type="gene ID" value="SSO0162"/>
    </dbReference>
    <dbReference type="GeneID" id="1453334"/>
    <dbReference type="KEGG" id="sso:SSO0162"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.158"/>
    <dbReference type="eggNOG" id="arCOG01107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_2_0_2"/>
    <dbReference type="InParanoid" id="Q980W5"/>
    <dbReference type="PhylomeDB" id="Q980W5"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00039"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05653">
        <property type="entry name" value="M20_ArgE_LysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01120">
        <property type="entry name" value="LysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010175">
        <property type="entry name" value="LysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01902">
        <property type="entry name" value="dapE-lys-deAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001747">
        <property type="entry name" value="PRK00466.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF28">
        <property type="entry name" value="[LYSW]-LYSINE_[LYSW]-ORNITHINE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000185350" description="[LysW]-lysine/[LysW]-ornithine hydrolase">
        <location>
            <begin position="1"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01120"/>
        </source>
    </evidence>
    <sequence length="346" mass="38810" checksum="B09791618F366D82" modified="2001-10-01" version="1">MLQEKELVKQKAKELLLDLLSIYTPSKSEANATKFFEKISKDLNLKLEILPDSNSFILGEGDILLASHVDTVYGYIEPKIENELIYGRGAVDAKGPLISMIIATWLLNEKGIKVRVSGLADEESTSIGAKELIAKNYNFKYIIVGEPSNATDIVVEYRGSIQLDIMCEGTPEHSSSAKNNLIVDISKKIIEVYKQPENYDKPSIVPTIIRAGESYNVTPAKLYLHLDIRYAINNKREDLIKEITDKFPECNLKIVDETPPVKVSINNPVVKSLARALLKQNIKPRLVKKAGTSDMNILQRITTNIATYGPGNSMLEHTTQEKISLDEIYIGVKTYMLAIEELWQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q980X9</accession>
    <name>Q980X9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SSV1 integrase homolog (Orf D-335)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0143</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90154">
        <property type="entry name" value="F90154"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980X9"/>
    <dbReference type="STRING" id="273057.SSO0143"/>
    <dbReference type="PaxDb" id="273057-SSO0143"/>
    <dbReference type="EnsemblBacteria" id="AAK40493">
        <property type="protein sequence ID" value="AAK40493"/>
        <property type="gene ID" value="SSO0143"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0143"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.140"/>
    <dbReference type="eggNOG" id="arCOG01244">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083804_0_0_2"/>
    <dbReference type="InParanoid" id="Q980X9"/>
    <dbReference type="PhylomeDB" id="Q980X9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031857">
        <property type="entry name" value="Integrase_SSV1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16795">
        <property type="entry name" value="Phage_integr_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase SSV1 C-terminal" evidence="2">
        <location>
            <begin position="108"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase SSV1 C-terminal" evidence="2">
        <location>
            <begin position="180"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="290" mass="32955" checksum="FA5B0BBF9DA0F0AC" modified="2001-10-01" version="1">MNPGSSPCEGSKKGVERCKMTLVAILRQYSADYAQEFYDYLLNERKIGNKTAKEYVSAVNRPYKETDKSQKAYRIFAKFLESRGVISSEEMEKILRVIKVKRSNADLYIPTEEGIRKTLKLAKEYSENVYFVYRIALESGARLNEILKVLKKPEKDVCDSADDSNGRIRSNGSADGGISLCYYPLSWIRGSKGSFYVFHFSPLRKVTIGEGAIAGFERRRDGAVAIKYVRKFVASKMAELGIPLDVIDFIQGRKPTRVLTQHYISLFGIAKEHYRKYAEWLSKSGVLSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q981B6</accession>
    <name>Q981B6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90142">
        <property type="entry name" value="E90142"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989610.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981B6"/>
    <dbReference type="FunCoup" id="Q981B6">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0031"/>
    <dbReference type="PaxDb" id="273057-SSO0031"/>
    <dbReference type="EnsemblBacteria" id="AAK40396">
        <property type="protein sequence ID" value="AAK40396"/>
        <property type="gene ID" value="SSO0031"/>
    </dbReference>
    <dbReference type="GeneID" id="1455287"/>
    <dbReference type="KEGG" id="sso:SSO0031"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.32"/>
    <dbReference type="eggNOG" id="arCOG00062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_3_8_2"/>
    <dbReference type="InParanoid" id="Q981B6"/>
    <dbReference type="PhylomeDB" id="Q981B6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07197">
        <property type="entry name" value="nitrilase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001110">
        <property type="entry name" value="UPF0012_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF27">
        <property type="entry name" value="DEAMINATED GLUTATHIONE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01227">
        <property type="entry name" value="UPF0012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="238" mass="26958" checksum="401C841F13280F86" modified="2001-10-01" version="1">MKIGVVQPLEVKSAILLAEKALKEGAELVLLPEKWTRNIDDVPLLEFQKLAKRYTAYILPGAFEDGVSVITPIIDDSGNLKGIAKKIHLFNEERVRLIPGNEAVLFTYRGIRFGISICYDIDFPEVVRELFSKGAEIILVPSKIRSNGLDIWNEFLRIRVLENRIGIVNANVYNPPDFPGRSVAIVPEQRQDGIVIPKVVGELGSEESYMIVDIDPLKYMYLRGDRLKEYVKFTVKEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX78</accession>
    <name>AMZA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Archaemetzincin</fullName>
            <ecNumber evidence="1">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">amzA</name>
        <name type="ordered locus">SSO0689</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable zinc metalloprotease whose natural substrate is unknown.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit. One is catalytic, whereas the other seems to have a structural role.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90216">
        <property type="entry name" value="F90216"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991235.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX78"/>
    <dbReference type="SMR" id="Q9UX78"/>
    <dbReference type="STRING" id="273057.SSO0689"/>
    <dbReference type="PaxDb" id="273057-SSO0689"/>
    <dbReference type="EnsemblBacteria" id="AAK40989">
        <property type="protein sequence ID" value="AAK40989"/>
        <property type="gene ID" value="SSO0689"/>
    </dbReference>
    <dbReference type="GeneID" id="1454956"/>
    <dbReference type="KEGG" id="sso:SSO0689"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.688"/>
    <dbReference type="eggNOG" id="arCOG00458">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108521_2_0_2"/>
    <dbReference type="InParanoid" id="Q9UX78"/>
    <dbReference type="PhylomeDB" id="Q9UX78"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11375">
        <property type="entry name" value="Peptidase_M54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01842">
        <property type="entry name" value="Archaemetzincin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012962">
        <property type="entry name" value="Pept_M54_archaemetzincn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012091">
        <property type="entry name" value="Pept_M54_archaemetzncn_arc/bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033823">
        <property type="entry name" value="archmetzin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15910">
        <property type="entry name" value="ARCHAEMETZINCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15910:SF1">
        <property type="entry name" value="ARCHAEMETZINCIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07998">
        <property type="entry name" value="Peptidase_M54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005785">
        <property type="entry name" value="Zn-prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000159636" description="Archaemetzincin">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01842"/>
        </source>
    </evidence>
    <sequence length="183" mass="21340" checksum="AB9C9E27F294F9B0" modified="2000-05-01" version="1">MTEMKILIVTLTYIEKSIIDEIVNNLSSYGLEVDILLDSRKYLPISAFNWERLQYDAEKVLSFLKSIHDFNYDSIIFLADSDGYIDGYNFVFGLTIDNFAIIFLHRLREEFYNRKPDLDLFMKRVVKEVTHEVGHTLGLSHCNTTGCVMNFSNSVEDVDKKQAKFCKNCAHKIEKLSKYLQQK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX85</accession>
    <name>RL15_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L15</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl15</name>
        <name type="synonym">rpl15Ab</name>
        <name type="ordered locus">SSO0696</name>
        <name type="ORF">C10_034</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90217">
        <property type="entry name" value="F90217"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991250.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX85"/>
    <dbReference type="SMR" id="Q9UX85"/>
    <dbReference type="FunCoup" id="Q9UX85">
        <property type="interactions" value="203"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0696"/>
    <dbReference type="PaxDb" id="273057-SSO0696"/>
    <dbReference type="EnsemblBacteria" id="AAK40997">
        <property type="protein sequence ID" value="AAK40997"/>
        <property type="gene ID" value="SSO0696"/>
    </dbReference>
    <dbReference type="GeneID" id="1454964"/>
    <dbReference type="KEGG" id="sso:SSO0696"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.696"/>
    <dbReference type="eggNOG" id="arCOG00779">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109163_0_0_2"/>
    <dbReference type="InParanoid" id="Q9UX85"/>
    <dbReference type="PhylomeDB" id="Q9UX85"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.990.10:FF:000001">
        <property type="entry name" value="50S ribosomal protein L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.990.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027386">
        <property type="entry name" value="Rbsml_uL15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_uL15_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000104875" description="Large ribosomal subunit protein uL15">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="144" mass="16197" checksum="ED83FC5815B3AB81" modified="2000-05-01" version="1">MVVRREKKSRKMRGSRTMGWGIRGQHRDRGSQGGRQIGMHKEKWSWLVKYGKGWYGKHGFRNPTTKLTSAISLRKLNELLESGYIKIKEMDGKKIVDLNELGYNKLLGGGSISIPVTIKVGKATNKAIQKVKEMGGEVILSPTE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2025-06-18" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>O33771</accession>
    <name>HIS1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>ATP phosphoribosyltransferase</fullName>
            <shortName>ATP-PRT</shortName>
            <shortName>ATP-PRTase</shortName>
            <ecNumber>2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hisG</name>
        <name type="ordered locus">SSO0593</name>
        <name type="ORF">C08_057</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="J. Bacteriol." volume="179" first="4429" last="4432">
            <title>Evolutionary analysis of the hisCGABdFDEHI gene cluster from the archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Sensen C.W."/>
                <person name="Doolittle W.F."/>
                <person name="Brown J.R."/>
            </authorList>
            <dbReference type="PubMed" id="9209067"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Feedback inhibited by histidine.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP phosphoribosyltransferase family. Long subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17"/>
    <dbReference type="EMBL" id="U82227">
        <property type="protein sequence ID" value="AAB63019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90206">
        <property type="entry name" value="B90206"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O33771"/>
    <dbReference type="SMR" id="O33771"/>
    <dbReference type="FunCoup" id="O33771">
        <property type="interactions" value="189"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0593"/>
    <dbReference type="PaxDb" id="273057-SSO0593"/>
    <dbReference type="EnsemblBacteria" id="AAK40905">
        <property type="protein sequence ID" value="AAK40905"/>
        <property type="gene ID" value="SSO0593"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0593"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.601"/>
    <dbReference type="eggNOG" id="arCOG02208">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038115_1_1_2"/>
    <dbReference type="InParanoid" id="O33771"/>
    <dbReference type="PhylomeDB" id="O33771"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13594">
        <property type="entry name" value="PBP2_HisGL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.120:FF:000002">
        <property type="entry name" value="ATP phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00079">
        <property type="entry name" value="HisG_Long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020621">
        <property type="entry name" value="ATP-PRT_HisG_long"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018198">
        <property type="entry name" value="ATP_PRibTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013115">
        <property type="entry name" value="HisG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03455">
        <property type="entry name" value="HisG_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403:SF10">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE ATP-PRTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08029">
        <property type="entry name" value="HisG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01316">
        <property type="entry name" value="ATP_P_PHORIBOSYLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000151889" description="ATP phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="290" mass="31924" checksum="ADA3B3F611A4D7A7" modified="1998-01-01" version="1">MVRNLKIAIPNKGRLQQPTLQFLQSVGIKPLASDDRALIVPTSWEGVQLVMIRTEDIPNIVETGATELGITGHDYVIESSADVEELIKLDFGRSKIVLAVPQTWRENSVDELKGKEFRVATKYYNIAKEYVRRRELNAKVVKISGAAEVMPSLGAAEAIIDVMSTGTTLKLHGLKAIDVIMDSYAVVIGNRNWIKNDEADRINLLLTMMKGAIAAKGKKMIFMNVPDNKLDGVINSLPAMLAPAITRLSRSDIWEVITVAEEDILPEVIAKVKAAGARDIVVIDIEKVVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>O33774</accession>
    <name>HIS6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Imidazole glycerol phosphate synthase subunit HisF</fullName>
            <ecNumber>4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>IGP synthase cyclase subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>IGP synthase subunit HisF</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>ImGP synthase subunit HisF</fullName>
            <shortName>IGPS subunit HisF</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hisF</name>
        <name type="ordered locus">SSO0597</name>
        <name type="ORF">C08_052</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="J. Bacteriol." volume="179" first="4429" last="4432">
            <title>Evolutionary analysis of the hisCGABdFDEHI gene cluster from the archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Sensen C.W."/>
                <person name="Doolittle W.F."/>
                <person name="Brown J.R."/>
            </authorList>
            <dbReference type="PubMed" id="9209067"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.10"/>
    <dbReference type="EMBL" id="U82227">
        <property type="protein sequence ID" value="AAB63022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90206">
        <property type="entry name" value="E90206"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991116.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O33774"/>
    <dbReference type="SMR" id="O33774"/>
    <dbReference type="FunCoup" id="O33774">
        <property type="interactions" value="232"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0597"/>
    <dbReference type="PaxDb" id="273057-SSO0597"/>
    <dbReference type="EnsemblBacteria" id="AAK40908">
        <property type="protein sequence ID" value="AAK40908"/>
        <property type="gene ID" value="SSO0597"/>
    </dbReference>
    <dbReference type="GeneID" id="1454875"/>
    <dbReference type="GeneID" id="44129598"/>
    <dbReference type="KEGG" id="sso:SSO0597"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.604"/>
    <dbReference type="eggNOG" id="arCOG00617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_4_0_2"/>
    <dbReference type="InParanoid" id="O33774"/>
    <dbReference type="PhylomeDB" id="O33774"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000006">
        <property type="entry name" value="Imidazole glycerol phosphate synthase subunit HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01013">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050064">
        <property type="entry name" value="IGPS_HisA/HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00735">
        <property type="entry name" value="hisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235:SF2">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE HISHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISF/H IGP SYNTHASE SUBUNIT HISF/H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142290" description="Imidazole glycerol phosphate synthase subunit HisF">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="251" mass="26965" checksum="46246934AC1BA850" modified="1998-01-01" version="1">MTTKRIIACLDVKDGNVVKGVNFLNLQLKGDPVSLASLYEEEGADEIVFLDITATIEARKALYNVIKDTASVLSIPLTVGGGIRTPDDVSMALRSGADKVSINTAAVESSQIVKKSAEEFGSQAVVVAIDVKKVSGNWIVFTKSGTYNTRLDAIKWAKKVEELGAGEILLTSIDRDGTRLGYDLELTRKIVDSVNIPVIASGGAGKMEHFYEVFSLAKADAALAAGIFHDGIIKIKDLKSYLSQKGIEVRM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-20" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>P58190</accession>
    <name>RL31_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>50S ribosomal protein L31e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rpl31e</name>
        <name type="ordered locus">SSO5671</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90178">
        <property type="entry name" value="C90178"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990647.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58190"/>
    <dbReference type="SMR" id="P58190"/>
    <dbReference type="FunCoup" id="P58190">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO5671"/>
    <dbReference type="PaxDb" id="273057-SSO5671"/>
    <dbReference type="EnsemblBacteria" id="AAK40682">
        <property type="protein sequence ID" value="AAK40682"/>
        <property type="gene ID" value="SSO5671"/>
    </dbReference>
    <dbReference type="GeneID" id="1455492"/>
    <dbReference type="KEGG" id="sso:SSO5671"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.343"/>
    <dbReference type="eggNOG" id="arCOG04473">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2461923_0_0_2"/>
    <dbReference type="InParanoid" id="P58190"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00463">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00410">
        <property type="entry name" value="Ribosomal_eL31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_eL31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023621">
        <property type="entry name" value="Ribosomal_eL31_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002258">
        <property type="entry name" value="PRK01192.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54575">
        <property type="entry name" value="Ribosomal protein L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000153806" description="Large ribosomal subunit protein eL31">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="88" mass="10176" checksum="F4A864E4270C3D68" modified="2001-06-20" version="1">MKEKDNFEMIINLRKIKTGKRTGRSKRAVKFIKKVVARHFNAEKVIIDPLLAKSISKNGNDKIVSKIRVVVSKIEEKIYLVRLAIKSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2026-01-28" version="151" xmlns="https://uniprot.org/uniprot">
    <accession>P95907</accession>
    <name>GLPK2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycerol kinase 2</fullName>
            <ecNumber evidence="1">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP:glycerol 3-phosphotransferase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycerokinase 2</fullName>
            <shortName evidence="1">GK 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glpK2</name>
        <name type="synonym">glpK</name>
        <name type="ordered locus">SSO2133</name>
        <name type="ORF">C01009</name>
        <name type="ORF">C02_028</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycerol + ATP = sn-glycerol 3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="1"/>
    <dbReference type="EMBL" id="Y08256">
        <property type="protein sequence ID" value="CAA69459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74045">
        <property type="entry name" value="S74045"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988327.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95907"/>
    <dbReference type="SMR" id="P95907"/>
    <dbReference type="FunCoup" id="P95907">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2133"/>
    <dbReference type="PaxDb" id="273057-SSO2133"/>
    <dbReference type="EnsemblBacteria" id="AAK42312">
        <property type="protein sequence ID" value="AAK42312"/>
        <property type="gene ID" value="SSO2133"/>
    </dbReference>
    <dbReference type="GeneID" id="1453639"/>
    <dbReference type="GeneID" id="44130846"/>
    <dbReference type="KEGG" id="sso:SSO2133"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2225"/>
    <dbReference type="eggNOG" id="arCOG00024">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_2_3_2"/>
    <dbReference type="InParanoid" id="P95907"/>
    <dbReference type="PhylomeDB" id="P95907"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07786">
        <property type="entry name" value="FGGY_EcGK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000007">
        <property type="entry name" value="Glycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000008">
        <property type="entry name" value="Glycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00186">
        <property type="entry name" value="Glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005999">
        <property type="entry name" value="Glycerol_kin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01311">
        <property type="entry name" value="glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000756">
        <property type="entry name" value="PRK00047.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF69">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0319">Glycerol metabolism</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000059534" description="Glycerol kinase 2">
        <location>
            <begin position="1"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>sn-glycerol 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
            <position position="15"/>
        </location>
        <ligand>
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        </ligand>
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        </location>
        <ligand>
            <name>ADP</name>
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        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>glycerol</name>
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        </ligand>
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        </ligand>
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        </location>
        <ligand>
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        </ligand>
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        </ligand>
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        </location>
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        </ligand>
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            <position position="134"/>
        </location>
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            <name>sn-glycerol 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
        </ligand>
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        </location>
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        </ligand>
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        </location>
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        </ligand>
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        </location>
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        </ligand>
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            <position position="306"/>
        </location>
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            <name>ADP</name>
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        </location>
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        <ligand>
            <name>ATP</name>
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        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>ADP</name>
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        </ligand>
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        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00186"/>
        </source>
    </evidence>
    <sequence length="499" mass="55643" checksum="932B3096774E5FAC" modified="1997-05-01" version="1">MPGGFILAIDEGTTSARAIIYNQDLEVLGIGQYDFPQHYPSPGYVEHNPDEIWNAQMLAIKEAMKKAKIESRQVAGIGVTNQRETTILWDAISGKPIYNAIVWQDRRTSNITDWLKENYFGMIKDKTGLIPDPYFSGSKIKWILDNLPNVRSKAEKGEIKFGTIDTYLIWKLTNGKIHVTDYSNASRTMLFNINKLEWDREILELLKIPESILPEVRPSSDIYGYTEVLGSSIPISGDAGDQQAALFGQVAYDMGEVKSTYGTGSFILMNIGSNPIFSENLLTTIAWGLESKRVTYALEGSIFITGAAVQWFRDGLRAIDASDDIEPLAASVPDTGGVYFVPAFVGLGAPYWDPYARGLIIGITRGTTKAHIARAILESIAYQNRDVIEIMEKESGTKINILKVDGGGAKDNLLMQFQADILGIRVVRPKVMETASMGVAMLAGLAINYWNSLNELKQKWTVDKEFIPSINKEERERRYNAWKEAVKRSLGWEKSLGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX69</accession>
    <name>Q7LX69_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="4">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">porA-like</name>
        <name evidence="9" type="ordered locus">SSO2129</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74041">
        <property type="entry name" value="S74041"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988321.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX69"/>
    <dbReference type="FunCoup" id="Q7LX69">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2129"/>
    <dbReference type="PaxDb" id="273057-SSO2129"/>
    <dbReference type="DNASU" id="1453635"/>
    <dbReference type="EnsemblBacteria" id="AAK42308">
        <property type="protein sequence ID" value="AAK42308"/>
        <property type="gene ID" value="SSO2129"/>
    </dbReference>
    <dbReference type="GeneID" id="1453635"/>
    <dbReference type="KEGG" id="sso:SSO2129"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2221"/>
    <dbReference type="eggNOG" id="arCOG01608">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002569_5_0_2"/>
    <dbReference type="InParanoid" id="Q7LX69"/>
    <dbReference type="PhylomeDB" id="Q7LX69"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000010">
        <property type="entry name" value="Pyruvate ferredoxin oxidoreductase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pyruvate:ferredoxin (Flavodoxin) oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF30">
        <property type="entry name" value="2-OXOACID OXIDOREDUCTASE (FERREDOXIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="7">
        <location>
            <begin position="22"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate:ferredoxin oxidoreductase core" evidence="8">
        <location>
            <begin position="280"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="415" mass="46708" checksum="EFCB461C8648B3FF" modified="2011-05-31" version="1">MTEVKKLVEREVLMNGTQAVAHAAMYADVDVVAAYPIRPYTEVMDTISKLIADGELDAEFIVAEGEHGQFETVKHASLAGARTLVGSSGVGWLYAMEAIVVTATDRAPVVAIIGNRALDDPGAYGVEHNDALMVRDVGWLLVWVDTAQEAFDTALLAYRIAEDQRVLLPLGISMDGGFLTHSEQIVRLPPRELVKNFLPPYNRGKYLVHPDNPITVAPQVNEDWVMEIRRQHEEAMERARSVIVEAYEEFKKVFGRYPGSTSDLQMPENPFVEPYMIDDAEVVLIGMGTVSKPMKVAIKNMRRQGYKVGMLRIRWFRPFPTQDVIRYLANSRVVCVIDRDYSMGSPNRGGVIYHEVRSSLYDLDQRPRVMNFIGGLGGREITIQDVEKIIKIGYEHRDTPITKPVYWVGVRGDPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX95</accession>
    <name>Q7LX95_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74013">
        <property type="entry name" value="S74013"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923772.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX95"/>
    <dbReference type="PaxDb" id="273057-SSO2100"/>
    <dbReference type="EnsemblBacteria" id="AAK42279">
        <property type="protein sequence ID" value="AAK42279"/>
        <property type="gene ID" value="SSO2100"/>
    </dbReference>
    <dbReference type="GeneID" id="1453606"/>
    <dbReference type="KEGG" id="sso:SSO2100"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2182"/>
    <dbReference type="eggNOG" id="arCOG09933">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2165380_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX95"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="110" mass="12652" checksum="E19CA8430137D169" modified="2011-05-31" version="1">MAVALRGLGRIKESEKFVESLREGLKKYDYEFYFSAHSLVSYHSIFNEIDEVDKLIDSIERIKHGDKGTNIMLYALSANTAYAYTKKERYLDIALEAFQKSKGNERVSGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXE4</accession>
    <name>Q7LXE4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0834</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90234">
        <property type="entry name" value="B90234"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXE4"/>
    <dbReference type="PaxDb" id="273057-SSO0834"/>
    <dbReference type="EnsemblBacteria" id="AAK41129">
        <property type="protein sequence ID" value="AAK41129"/>
        <property type="gene ID" value="SSO0834"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0834"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.850"/>
    <dbReference type="HOGENOM" id="CLU_1431706_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXE4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="189" mass="20806" checksum="7855C450C67E59A7" modified="2011-05-31" version="1">MSCFIYRIKMKMTTTKGRFNEKSIESLSMGIGIISVVVLLTTGGSISIASMVLLSTAIIFPISFLLITKRNINRLVIRLTRPIINASGFDDKKLEIGNVLLLLLVGLLVIIYIIVKCIPKAFEEYISTVSPSNPLYNSIMTIMTIYKPWQGLGSLFSSPIGLIIIILIVVMIAVIIALKKRSFSSYAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXF8</accession>
    <name>Q7LXF8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0819</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90232">
        <property type="entry name" value="D90232"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923110.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXF8"/>
    <dbReference type="FunCoup" id="Q7LXF8">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0819"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO0819"/>
    <dbReference type="EnsemblBacteria" id="AAK41115">
        <property type="protein sequence ID" value="AAK41115"/>
        <property type="gene ID" value="SSO0819"/>
    </dbReference>
    <dbReference type="GeneID" id="1455082"/>
    <dbReference type="KEGG" id="sso:SSO0819"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.831"/>
    <dbReference type="eggNOG" id="arCOG01419">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_793709_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXF8"/>
    <dbReference type="PhylomeDB" id="Q7LXF8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004378">
        <property type="term" value="F:GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027054">
        <property type="entry name" value="ALG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45918">
        <property type="entry name" value="ALPHA-1,3/1,6-MANNOSYLTRANSFERASE ALG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45918:SF1">
        <property type="entry name" value="ALPHA-1,3_1,6-MANNOSYLTRANSFERASE ALG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="231"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="349" mass="39692" checksum="6C5C1E60F0825236" modified="2011-05-31" version="1">MKIFFLTSRLKERDGASRAIINFSKGIKQISRYEPVIISLSTSIGVNKLGNIEIINIDKGLGLLSSFRILLGSPSPLKPFKKYLHDDGIYIVAVEDIIPVSEFKDDLIYWSQGVLTSVFFWEPFIRRNKLVSALASPFLLYNAIKFSNFVKKYKVVLANSKTSAVYVSLLYNRPPKAVVYPPLNTDFFKPSKNKEKFVLVFLKRGYPSHVDLLTKIAEKVKMKSIGYKIPNAEYLERVSDEELRDLYSSALVTLYPIDFEYYGYIPVESMAAGTPVIAFRYSGGPAETIVDGETGWLASDEEEFYRLTIKVYNEGYPEDTSVKSRKRAEEFSIQNQTKLLLSIIEKLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-01-24" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXI2</accession>
    <name>Q7LXI2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0791</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99229">
        <property type="entry name" value="B99229"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXI2"/>
    <dbReference type="STRING" id="273057.SSO0791"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO0791"/>
    <dbReference type="EnsemblBacteria" id="AAK41089">
        <property type="protein sequence ID" value="AAK41089"/>
        <property type="gene ID" value="SSO0791"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0791"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.797"/>
    <dbReference type="eggNOG" id="arCOG05431">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_782162_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXI2"/>
    <dbReference type="PhylomeDB" id="Q7LXI2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="206"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="355" mass="40792" checksum="40CAF835F1DC1169" modified="2011-05-31" version="1">MMFTIALRVLWNGGVARIAVEQARNAKAKLLVYRESFYNYDLSGIYVEFLRRKGEKGFLTPLFRRITEIYAKNRGDEATVDLDLIVKASRIIKGPALFHDQFAGITGYLRKVFDGEDYAIYLHETSLTLKGIKYLIPKVMERKVLEDAKIVLTNSQWNKRILEDKGIKAEVLYPGCYPIDKIEEDKENFVLTVSMWDEGRKPEIYGEIAKRIKGKLIMAGSWARRDTMEEFKRKYPEVIVTGNISEDKLRELYKKASLTIRFGFNERGPGMAVLESLCNGTAVIANDGLGGKEFVIEGENGYVVKDWKEAVDRINEVLENHKLRRSLSINALETSKKYSWRNHANKLTEIMEKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXM0</accession>
    <name>Q7LXM0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90220">
        <property type="entry name" value="D90220"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXM0"/>
    <dbReference type="STRING" id="273057.SSO0720"/>
    <dbReference type="PaxDb" id="273057-SSO0720"/>
    <dbReference type="EnsemblBacteria" id="AAK41019">
        <property type="protein sequence ID" value="AAK41019"/>
        <property type="gene ID" value="SSO0720"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0720"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.719"/>
    <dbReference type="eggNOG" id="arCOG04069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1145238_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXM0"/>
    <dbReference type="PhylomeDB" id="Q7LXM0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18086">
        <property type="entry name" value="HsC9orf114-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003750">
        <property type="entry name" value="Put_MeTrfase-C9orf114-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02598">
        <property type="entry name" value="Methyltrn_RNA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="265" mass="30600" checksum="410E0A8AAD13A265" modified="2011-05-31" version="1">MGYRRGILYNRDKEVYMMFPFPRYKPLNVVLFLSIFDVENSLLEVTLKLSFILRVTTTFRVNKIYWITDSLNSRKLEKIITDITKYALLPPYLKKYVPISRNLKKVGLMSPLGIPYHFIFKQAVEGEIRLGYKGDFGLNKKLNSSSKTILITDSLKAGYIDYRGFYYTGVKNEFIDFKKVFNFDNLIIASRSGKNPLQAKEQLTNLYNKYGLTLMIGPPTGNLLQRLGKQYLDKSYNFVIKQGVSDIRAEEALAYSLSILNLLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXQ2</accession>
    <name>Q7LXQ2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO0653</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90212">
        <property type="entry name" value="G90212"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991191.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXQ2"/>
    <dbReference type="STRING" id="273057.SSO0653"/>
    <dbReference type="PaxDb" id="273057-SSO0653"/>
    <dbReference type="EnsemblBacteria" id="AAK40958">
        <property type="protein sequence ID" value="AAK40958"/>
        <property type="gene ID" value="SSO0653"/>
    </dbReference>
    <dbReference type="GeneID" id="1454925"/>
    <dbReference type="KEGG" id="sso:SSO0653"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.655"/>
    <dbReference type="eggNOG" id="arCOG00946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044176_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LXQ2"/>
    <dbReference type="PhylomeDB" id="Q7LXQ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027596">
        <property type="entry name" value="AmmeMemoSam_rS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04337">
        <property type="entry name" value="AmmeMemoSam_rS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352:SF5">
        <property type="entry name" value="PYRUVATE FORMATE-LYASE 1-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PYRUVATE FORMATE-LYASE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01101">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="67"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004869-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK40958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="351" mass="40265" checksum="C84729B9A06A8DB1" modified="2011-05-31" version="1">MTKEAVLYKKEDGRIRCLACARKCLIGEGQVGFCGVRSVRNGKLYLDVYGKIAAAHIDPIEKKPLVHFNPGSKVFSFSTYGCNWMCMYCQNYDISQRRKAEGVELSPEEIVELAMGYDVDGMTYTYNEPAIFSEFAHDVGIVAKRYGLFNTMVSNGYWTPNLVDYVKDFLDAVTIDFKGNGEQKFLRRYTGATSAEPIIETASELSKKGIHVEITDLIIPQIGDDLQAAKQLLTKLYDQLGADIPIHFLRFHPDYKLNYIPWTPVETLERHYKLAKELGFKFVYVGNVPGHPYENTYCPNCGNLVIRRYSFDILEWHLTEDMRCKFCGYKLPIRGKLSRHAFKERFEPVFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-02-05" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXX3</accession>
    <name>Q7LXX3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0518</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90197">
        <property type="entry name" value="E90197"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXX3"/>
    <dbReference type="STRING" id="273057.SSO0518"/>
    <dbReference type="PaxDb" id="273057-SSO0518"/>
    <dbReference type="EnsemblBacteria" id="AAK40836">
        <property type="protein sequence ID" value="AAK40836"/>
        <property type="gene ID" value="SSO0518"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0518"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.512"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987729_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXX3"/>
    <dbReference type="PhylomeDB" id="Q7LXX3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="125" mass="14491" checksum="956C877AB217FD05" modified="2011-05-31" version="1">MVDLVTEAKSSGEGVTSPYSQTEHCENIHEYRCDTRVGVIGIDVSKEHLVTSKGRVRKFTNNEKGYEEILTMKPNIIVIEPTRVYSTRPCQYFKERGVKVLLVSTTLLWKEKDVRGKRFLRRRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U22</accession>
    <name>Q97U22_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acetate--CoA ligase</fullName>
            <ecNumber evidence="7">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">acsA-10</name>
        <name evidence="11" type="ordered locus">SSO3203</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in the autotrophic CO(2) fixation pathway. Activates 3-hydroxypropionate to its CoA ester. Can also activate propionate, and to a lesser extent acrylate, acetate and butyrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-hydroxypropanoate + ATP + CoA = 3-hydroxypropanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:26534"/>
            <dbReference type="ChEBI" id="CHEBI:16510"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.36"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90505">
        <property type="entry name" value="E90505"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991677.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U22"/>
    <dbReference type="FunCoup" id="Q97U22">
        <property type="interactions" value="316"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3203"/>
    <dbReference type="PaxDb" id="273057-SSO3203"/>
    <dbReference type="EnsemblBacteria" id="AAK43300">
        <property type="protein sequence ID" value="AAK43300"/>
        <property type="gene ID" value="SSO3203"/>
    </dbReference>
    <dbReference type="GeneID" id="1453219"/>
    <dbReference type="KEGG" id="sso:SSO3203"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3305"/>
    <dbReference type="eggNOG" id="arCOG01529">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_2"/>
    <dbReference type="InParanoid" id="Q97U22"/>
    <dbReference type="PhylomeDB" id="Q97U22"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043955">
        <property type="term" value="F:3-hydroxypropionyl-CoA synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043427">
        <property type="term" value="P:carbon fixation by 3-hydroxypropionate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF232">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="10">
        <location>
            <begin position="23"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="8">
        <location>
            <begin position="88"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="9">
        <location>
            <begin position="532"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR02188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK43300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="652" mass="73724" checksum="3C2C364943159FE0" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U54</accession>
    <name>Q97U54_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transporter, ATP binding protein related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO3169</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90501">
        <property type="entry name" value="E90501"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989979.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U54"/>
    <dbReference type="FunCoup" id="Q97U54">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3169"/>
    <dbReference type="PaxDb" id="273057-SSO3169"/>
    <dbReference type="EnsemblBacteria" id="AAK43268">
        <property type="protein sequence ID" value="AAK43268"/>
        <property type="gene ID" value="SSO3169"/>
    </dbReference>
    <dbReference type="GeneID" id="1453187"/>
    <dbReference type="KEGG" id="sso:SSO3169"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3273"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q97U54"/>
    <dbReference type="PhylomeDB" id="Q97U54"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43582">
        <property type="entry name" value="LINEARMYCIN RESISTANCE ATP-BINDING PROTEIN LNRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43582:SF2">
        <property type="entry name" value="LINEARMYCIN RESISTANCE ATP-BINDING PROTEIN LNRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="2"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="275" mass="30607" checksum="CD7846C9E7E19166" modified="2001-10-01" version="1">MLETINLTKIYRDGTVALDNLTFSANTRVVTLLGRNGAGKTTLTRILSTQLLPTSGIARVEGYDVVKDARKVRKIIASIPQEAKSIGIASPMEHLIMYLTARGLSFKEATEISRSALKEVGLWEVRDKPSDELSGGMKRKIFVAMALASNAELVLLDEPTTGLDPYSRLEVWSVLKSIKSKILLTTHYMEEAEELSDEVILLHKGKLIAKGSIKELLTKFDNKVRIEGIGEYKIGRLRISYVDRNEASDYVGKYVIKPITLEDLFIIYGGESLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U79</accession>
    <name>Q97U79_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carnitine dehydratase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90498">
        <property type="entry name" value="C90498"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990909.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3144"/>
    <dbReference type="PaxDb" id="273057-SSO3144"/>
    <dbReference type="EnsemblBacteria" id="AAK43242">
        <property type="protein sequence ID" value="AAK43242"/>
        <property type="gene ID" value="SSO3144"/>
    </dbReference>
    <dbReference type="GeneID" id="1453161"/>
    <dbReference type="KEGG" id="sso:SSO3144"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3248"/>
    <dbReference type="eggNOG" id="arCOG02304">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U79"/>
    <dbReference type="PhylomeDB" id="Q97U79"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008410">
        <property type="term" value="F:CoA-transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="entry name" value="Crotonobetainyl-coa:carnitine coa-transferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="entry name" value="formyl-coa transferase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050483">
        <property type="entry name" value="CoA-transferase_III_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48207">
        <property type="entry name" value="SUCCINATE--HYDROXYMETHYLGLUTARATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48207:SF3">
        <property type="entry name" value="SUCCINATE--HYDROXYMETHYLGLUTARATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="CoA-transferase family III (CaiB/BaiF)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="372" mass="42074" checksum="711893741BB72E2C" modified="2001-10-01" version="1">MDKIRVIELGSNISAPLVGTILADLGAEVIKVEPPPYGDDRRRVKPEINGISVYFASTNRGKKSVVINLKSDEGYEIFQRLVKTADVIVTNYRPSALKRLKVDYESVKRINPKIIYCSITGFGNLTEEADRPAYDPIILALSGLMDMTGEENPVKFATSISDITTGLLATVMILWALNKGGPTFIDVPMIYTQFYLTLEDAYMYLNTGKVPKRMGSAHRYLVPYQAFKVVDGYIYVAVFNDEQYLKLCKALNREDLCKFDTLQKRVENRDYLIKELGKIFEKNTRDYWVDLLSKADVPVAPVLSLEQAFKKYGNRLVYEIEGVKYIDFPINITSNKSKAPRLGENTKEVLLELGYSEGEINRLAEKGIIMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U95</accession>
    <name>Q97U95_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HD/PDEase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90496">
        <property type="entry name" value="C90496"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U95"/>
    <dbReference type="FunCoup" id="Q97U95">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3125"/>
    <dbReference type="PaxDb" id="273057-SSO3125"/>
    <dbReference type="EnsemblBacteria" id="AAK43226">
        <property type="protein sequence ID" value="AAK43226"/>
        <property type="gene ID" value="SSO3125"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3125"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3231"/>
    <dbReference type="eggNOG" id="arCOG04430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026821_3_1_2"/>
    <dbReference type="InParanoid" id="Q97U95"/>
    <dbReference type="PhylomeDB" id="Q97U95"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
        <property type="entry name" value="HD_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="1">
        <location>
            <begin position="52"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="413" mass="48029" checksum="2FC976E8661C20A8" modified="2001-10-01" version="1">MQILFFMKIIRDPIHGYIEVEDFVLQIISTEIFQRLRHITQTGLAYLVYPGMRHTRFEHSLGVMHLAKELTRYIKINSEQYTDLDFINEEYLKLVGLSGLLHDIGHLPFSHTFENALSLAKEVYGIDVEYYGKKTHVIFGNRVIDYYLGNYLDKLSKNYDVVNFVQRVISSTPRTKEESLASLIISSPLDADRGDYLLRDSYFAGVGYGNFDIERIKRSLIYVNGKLAVLKKAIPVVEQFLLARMYMYETIYFHSVVGLYNAVLSHAVVKLIQKNLIPNVAPENYLKLNDVVIMSKLNEAGREFFDAIVYRKGFKRYKKDLTGNCYETLEKKRKEINEIMRENNGLILYHDFYDVPYSEDEIFVYDDDELKPLSTESKIVSSLTIIKKGIVGYHESAEDKMKKIYEILQECPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UD0</accession>
    <name>Q97UD0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0033 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO11939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90491">
        <property type="entry name" value="H90491"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989107.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UD0"/>
    <dbReference type="FunCoup" id="Q97UD0">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO11939"/>
    <dbReference type="PaxDb" id="273057-SSO11939"/>
    <dbReference type="EnsemblBacteria" id="AAK43191">
        <property type="protein sequence ID" value="AAK43191"/>
        <property type="gene ID" value="SSO11939"/>
    </dbReference>
    <dbReference type="GeneID" id="15298661"/>
    <dbReference type="KEGG" id="sso:SSO11939"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3193"/>
    <dbReference type="eggNOG" id="arCOG02074">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165255_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UD0"/>
    <dbReference type="PhylomeDB" id="Q97UD0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00291">
        <property type="entry name" value="SirA_YedF_YeeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="entry name" value="TusA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279:SF18">
        <property type="entry name" value="SULFUR CARRIER PROTEIN MJ0990-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SirA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="1">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="82" mass="9291" checksum="706D678F065EB486" modified="2001-10-01" version="1">MEDLTLRQPDEVLDVRGEECPVPEMSASKKLKKMKVGQMLEVLTDHEPAVHVTLPSLCKSLGYPFVVIRDNGLYKVRILKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UE8</accession>
    <name>Q97UE8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO3074</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90489">
        <property type="entry name" value="F90489"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UE8"/>
    <dbReference type="PaxDb" id="273057-SSO3074"/>
    <dbReference type="EnsemblBacteria" id="AAK43173">
        <property type="protein sequence ID" value="AAK43173"/>
        <property type="gene ID" value="SSO3074"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3074"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3176"/>
    <dbReference type="eggNOG" id="arCOG00271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088838_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UE8"/>
    <dbReference type="PhylomeDB" id="Q97UE8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF76">
        <property type="entry name" value="EAMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="30"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="152"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="284" mass="31809" checksum="B11F4DA3BE4BBA14" modified="2001-10-01" version="1">MFSKNSLPIISRYSILKYLTDYFHNMRDKIILFLGGVAFGTAAIFVKFCTITPPFITFLRFLIAGILLLPFVSKRRRKIILKRFILPSLFLSLHMLFFVSSVFLTTIASSTILVSTSPIFAMLFKKKIDPFIIMAILGIFIMNFNTSFGYIFGNVLAILSAISFAIYTYFLSRLDYDSLSIIPIIYLISSIFMIPVLPFYGLGDINPISILAVLGLVLVPTLIGHGSVIYTANKLPISLVTSAELIEPVMATILAIPIFHQIPDTQELIGGTITLISIYFAFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UG7</accession>
    <name>Q97UG7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Maltose ABC transporter, maltose binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO3053</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90487">
        <property type="entry name" value="B90487"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924115.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UG7"/>
    <dbReference type="FunCoup" id="Q97UG7">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3053"/>
    <dbReference type="TCDB" id="3.A.1.5.8">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3053"/>
    <dbReference type="EnsemblBacteria" id="AAK43153">
        <property type="protein sequence ID" value="AAK43153"/>
        <property type="gene ID" value="SSO3053"/>
    </dbReference>
    <dbReference type="GeneID" id="1453072"/>
    <dbReference type="KEGG" id="sso:SSO3053"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3151"/>
    <dbReference type="eggNOG" id="arCOG01534">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_8_3_2"/>
    <dbReference type="InParanoid" id="Q97UG7"/>
    <dbReference type="PhylomeDB" id="Q97UG7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08509">
        <property type="entry name" value="PBP2_TmCBP_oligosaccharides_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.76.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290:SF9">
        <property type="entry name" value="OLIGOPEPTIDE-BINDING PROTEIN APPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="698"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="Solute-binding protein family 5" evidence="5">
        <location>
            <begin position="100"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="721" mass="80006" checksum="22BF58D32F953555" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-01-25" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UH6</accession>
    <name>GLCD2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucose 1-dehydrogenase 2</fullName>
            <shortName evidence="1">GDH 2</shortName>
            <shortName evidence="1">GlcDH 2</shortName>
            <ecNumber evidence="1">1.1.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gdh2</name>
        <name type="synonym">dhg-2</name>
        <name type="ordered locus">SSO3042</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NAD(P)(+)-dependent oxidation of D-glucose to D-gluconate via gluconolactone. Can utilize both NAD(+) and NADP(+) as electron acceptor. Is involved in the degradation of glucose through a non-phosphorylative variant of the Entner-Doudoroff pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose + NAD(+) = D-glucono-1,5-lactone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14293"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16217"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.47"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose + NADP(+) = D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14405"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16217"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit. One of the zinc atoms is essential for catalytic activity while the other has a structural function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family. Glucose 1-dehydrogenase subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK43143" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.47" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43143.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90485">
        <property type="entry name" value="H90485"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990696.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UH6"/>
    <dbReference type="SMR" id="Q97UH6"/>
    <dbReference type="FunCoup" id="Q97UH6">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3042"/>
    <dbReference type="PaxDb" id="273057-SSO3042"/>
    <dbReference type="EnsemblBacteria" id="AAK43143">
        <property type="protein sequence ID" value="AAK43143"/>
        <property type="gene ID" value="SSO3042"/>
    </dbReference>
    <dbReference type="GeneID" id="1453062"/>
    <dbReference type="KEGG" id="sso:SSO3042"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3140"/>
    <dbReference type="eggNOG" id="arCOG01459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_1_0_2"/>
    <dbReference type="InParanoid" id="Q97UH6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047934">
        <property type="term" value="F:glucose 1-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047935">
        <property type="term" value="F:glucose 1-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019595">
        <property type="term" value="P:non-phosphorylated glucose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08230">
        <property type="entry name" value="glucose_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02127">
        <property type="entry name" value="Glucose_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026583">
        <property type="entry name" value="Glc_1-DH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031640">
        <property type="entry name" value="Glu_dehyd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
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        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16912">
        <property type="entry name" value="Glu_dehyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000414841" description="Glucose 1-dehydrogenase 2">
        <location>
            <begin position="1"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
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        <location>
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        </location>
        <ligand>
            <name>substrate</name>
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            <position position="68"/>
        </location>
        <ligand>
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        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
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        <ligand>
            <name>substrate</name>
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            <position position="95"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
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        <ligand>
            <name>Zn(2+)</name>
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            <position position="101"/>
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            <name>Zn(2+)</name>
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        <location>
            <begin position="213"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
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        </ligand>
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            <begin position="279"/>
            <end position="281"/>
        </location>
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        </ligand>
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            <position position="309"/>
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    </feature>
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            <position position="356"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="368" mass="40890" checksum="E5774157751A9E5F" modified="2012-01-25" version="2">MKAIVVKPPNPGVEIKDVKDDENKLSNIGLVKVKILENGICGTDREIVSGKRTSVKPPIGKDELILGHEAIGIVEVGGYGFKEGELVMPINKRGCGKCLNCLIGRPDFCETGEGLVAGTKGLDGFMREYLYDDPKYLVKIPPAIKDIAILAQPLADIEKSVESILTSQKRIPIWTCDDGTLNCRKALVVGTGPTGILFSLVLRTYGFQVWIANRRELLDNEKEILDEPRILFYNSAKGYESLVKDVDKFDLIIDTTGASASIIQELVPLLQINGVLGLFGFPRYDNFSLDYKIVQDFVINNRIIIGLDNGQKPHFQQALIHLASWKSLWPKTASKMITKIISINDEREVISSLREKLPGEIKVKIVWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UJ1</accession>
    <name>Q97UJ1_SACS2</name>
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        <submittedName>
            <fullName evidence="2">Second ORF in transposase ISC1058</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">SSO3023</name>
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    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
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            <taxon>Archaea</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ISSOD12 TRANSPOSASE TNPA_ISSOD12"/>
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    </dbReference>
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        <property type="entry name" value="DDE_Tnp_1"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="14"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAK43125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="204" mass="23102" checksum="4007E3F9F8537FEE" modified="2001-10-01" version="1">MRNMKITFPEASDELEVIADATGISTNTGGQYIVAKWGKTRDSKFLKIEIVMDNNEFNVVNAEVTSNEVESAVKTVKDLQDKGKKVKKFYGDKTYDANEVYKTGVEVVVPPKKNASTKRGHLARRKAVREFRKLGYDRWREEKGYGVRWRVESLFSAVKRTFGESVRATSFAGQVVEAKLKFWAYAWMVHLANSVVGRAPGIRV</sequence>
</entry>
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    <accession>Q97UP5</accession>
    <name>Q97UP5_SACS2</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">SSO2959</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
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            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <property type="gene ID" value="SSO2959"/>
    </dbReference>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169421_0_0_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="115" mass="13620" checksum="F7501DED84D5BFED" modified="2001-10-01" version="1">MSLEGLGSWVNTYYNLEYTLDSMEEEFYYLFLTHDYTSYWVNKFRDVLEKLEEEVSMKQFLSILLYYLFSNSIKFNSLTIQTYSSVLLYFVLGNINFSFASPTVAENPDLLRSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UR7</accession>
    <name>Q97UR7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-methylhydantoinase B (HyuB-3)</fullName>
            <ecNumber evidence="2">3.5.2.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hyuB-3</name>
        <name evidence="2" type="ordered locus">SSO2934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.14" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90472">
        <property type="entry name" value="H90472"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991613.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UR7"/>
    <dbReference type="STRING" id="273057.SSO2934"/>
    <dbReference type="PaxDb" id="273057-SSO2934"/>
    <dbReference type="EnsemblBacteria" id="AAK43039">
        <property type="protein sequence ID" value="AAK43039"/>
        <property type="gene ID" value="SSO2934"/>
    </dbReference>
    <dbReference type="GeneID" id="1452958"/>
    <dbReference type="KEGG" id="sso:SSO2934"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3023"/>
    <dbReference type="eggNOG" id="arCOG01512">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020413_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UR7"/>
    <dbReference type="PhylomeDB" id="Q97UR7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047423">
        <property type="term" value="F:N-methylhydantoinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF23">
        <property type="entry name" value="HYPOTHETICAL 5-OXOPROLINASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="513" mass="56142" checksum="7BE2191837BA2347" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97US1</accession>
    <name>Q97US1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Partial transposase ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2924</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90471">
        <property type="entry name" value="H90471"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97US1"/>
    <dbReference type="PaxDb" id="273057-SSO2924"/>
    <dbReference type="EnsemblBacteria" id="AAK43031">
        <property type="protein sequence ID" value="AAK43031"/>
        <property type="gene ID" value="SSO2924"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2924"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113185_0_0_2"/>
    <dbReference type="InParanoid" id="Q97US1"/>
    <dbReference type="PhylomeDB" id="Q97US1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="136" mass="15163" checksum="AD3E0A1F47F9EB1E" modified="2001-10-01" version="1">MEKAVNEVILSMTGTRKDVAKRLVLGVVVGRNATEIAQEAEMDYETVLNNLDKAAQAKLIEVVKKLVGDHPVLLIIDDTHDHKLYARAMPVSRNGAQIFYCRAHKRFEPAIQLLVIGVKDLVNNQIYVSMGISFFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UV6</accession>
    <name>Q97UV6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2887</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90467">
        <property type="entry name" value="C90467"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UV6"/>
    <dbReference type="STRING" id="273057.SSO2887"/>
    <dbReference type="PaxDb" id="273057-SSO2887"/>
    <dbReference type="EnsemblBacteria" id="AAK42994">
        <property type="protein sequence ID" value="AAK42994"/>
        <property type="gene ID" value="SSO2887"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2887"/>
    <dbReference type="eggNOG" id="arCOG11994">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172293_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UV6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="101" mass="11678" checksum="A07DE99FF301C357" modified="2001-10-01" version="1">MRRRWIAPFVEIFKNTDYEIFLMTVTDGASVTETPWLKRGSMRYASAPDSDGTDVKYHAGWEPPLVPVELCPLPVLRYWHGRAVKQEAPSFTVDPKSFYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UW3</accession>
    <name>Q97UW3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF973 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90466">
        <property type="entry name" value="B90466"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989819.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UW3"/>
    <dbReference type="STRING" id="273057.SSO2880"/>
    <dbReference type="PaxDb" id="273057-SSO2880"/>
    <dbReference type="EnsemblBacteria" id="AAK42985">
        <property type="protein sequence ID" value="AAK42985"/>
        <property type="gene ID" value="SSO2880"/>
    </dbReference>
    <dbReference type="GeneID" id="1452904"/>
    <dbReference type="KEGG" id="sso:SSO2880"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2964"/>
    <dbReference type="eggNOG" id="arCOG03768">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237966_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UW3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009321">
        <property type="entry name" value="DUF973"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06157">
        <property type="entry name" value="DUF973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="24232" checksum="F4DBEEC9466A244D" modified="2001-10-01" version="1">MPQQNTEILGLEKIKKGVTYFLFYSILDIIVSVGVFITLVSVFHLPLTVNSMLAHYGIYAEIVLVIEAVLLPIFILSFWRIREGFKILATSRKELNKGTLGGTIGVIGYVLVLAGSIALIPVQLQQTVMPIAGRIVIGGYLLSLIGVLLIGLAYMKASNIYNNRTIKEGGTLILAWVLIAFIIGIAHSEPIAIFSEIFISSIGLLSAYLLSSGLGQVLRSKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UY7</accession>
    <name>Q97UY7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Proline/betaine transporter, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2852</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90463">
        <property type="entry name" value="B90463"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924002.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UY7"/>
    <dbReference type="FunCoup" id="Q97UY7">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2852"/>
    <dbReference type="PaxDb" id="273057-SSO2852"/>
    <dbReference type="EnsemblBacteria" id="AAK42961">
        <property type="protein sequence ID" value="AAK42961"/>
        <property type="gene ID" value="SSO2852"/>
    </dbReference>
    <dbReference type="GeneID" id="1452880"/>
    <dbReference type="KEGG" id="sso:SSO2852"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2937"/>
    <dbReference type="eggNOG" id="arCOG02691">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_5_2"/>
    <dbReference type="InParanoid" id="Q97UY7"/>
    <dbReference type="PhylomeDB" id="Q97UY7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045:SF1">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="5">
        <location>
            <begin position="13"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="446" mass="48705" checksum="790F02C31080E0FF" modified="2001-10-01" version="1">MEPEKFTWDSGKAIISQFIGFLLDSYDLTMILSIAPVLAKVILPPASPLIATFNIILSYSLTIIFRPLGSAIFGNLGDKIGRRADLIITVLGLGITSALTSALPTYAQAGILSFVLFVLIRVAVGIFAGGEYSAGHPFAMEWTPYKWRGFISGLVQGGFSFGAALAAVIEGIFIGIYGVSGVEEFAWRYVFLTAVIPAFIALAVRLAMKETPVFEDVKKKNLIRRTPLADLFKAPYRRDFLQVMAYMTGMFFFAYALFAFVPTILEHSPSTFSLGEANYIYSIGTYAAFAGALIFGTLSQYVGRRRLTIIWSIITFVIAIPIYYGLITAAIAGNAITAMLFSIIIGIITQGPWGIIPIYLSERFKASMRASGVGFGYSSGIFLGGWFSIYVPLMHNYLFKSLDTPNNVWFSTAVLLMIGALIVGIAQYFGPETLGTRLAEEPQKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V25</accession>
    <name>Q97V25_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2811</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90458">
        <property type="entry name" value="C90458"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923982.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V25"/>
    <dbReference type="STRING" id="273057.SSO2811"/>
    <dbReference type="PaxDb" id="273057-SSO2811"/>
    <dbReference type="EnsemblBacteria" id="AAK42922">
        <property type="protein sequence ID" value="AAK42922"/>
        <property type="gene ID" value="SSO2811"/>
    </dbReference>
    <dbReference type="GeneID" id="1452841"/>
    <dbReference type="KEGG" id="sso:SSO2811"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2896"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_2_2"/>
    <dbReference type="InParanoid" id="Q97V25"/>
    <dbReference type="PhylomeDB" id="Q97V25"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="178"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="304"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="411" mass="48101" checksum="50474DEC425B21BA" modified="2001-10-01" version="1">MPTLGFRFRAYTDEQTLRALKAQLKLACKIYNTLRWADIYFYQRDGKGLTQTELRQLALDLRKQDDEYKQLYSQVVQQIADRYYEAKKRFFEGLARFPKEKKPHKYYSLVYTQSGWKILHVREIRKGSKKNKKKLITLKLSNLGTFKVIVHRDFPLDKVKRVVVKLTRSERIYITFVVEGYEFPKLPNTGKVVAIDVGVEKLLVTPDGEYFPNLRPLEKALWKVKHLHRELSRKKFLSNNWFKAKVKLARAYEHLKNLRSDLYMKLGKWFAEHYDVVVMEGIHAKQLVGKSLRSLRRRLSDVGFGELRDVLKYQLEKYGKKLILVNPAYTSKTCARCGYVKNDLSLSDRVFVCPNCGWIADRDYNASLNILKGAGSERSLVPVELRPLPVPALRYWHGRAVKQEAPSFMRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V95</accession>
    <name>Q97V95_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2740</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90449">
        <property type="entry name" value="C90449"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990275.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V95"/>
    <dbReference type="FunCoup" id="Q97V95">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2740"/>
    <dbReference type="PaxDb" id="273057-SSO2740"/>
    <dbReference type="EnsemblBacteria" id="AAK42850">
        <property type="protein sequence ID" value="AAK42850"/>
        <property type="gene ID" value="SSO2740"/>
    </dbReference>
    <dbReference type="GeneID" id="1452769"/>
    <dbReference type="KEGG" id="sso:SSO2740"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2819"/>
    <dbReference type="eggNOG" id="arCOG00600">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1109541_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V95"/>
    <dbReference type="PhylomeDB" id="Q97V95"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="7"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="72"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="134"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="197"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="250" mass="27596" checksum="B500EE49174CAE4D" modified="2001-10-01" version="1">MKVIDIANPIVITIFPESSLKEAIDILSNNPSGRIVVLKEEKPIGIVSTRDVVASFSEYGTNIYDLKVKDIMSKDLVIVSPNDDVNHVVRIMLMNNIGGIPVVDNNVIVGIFTEREILKLIASSMFSGLVDSVMSSNIISIGEENTIMDAAKLMVMNNVRRLPVFSKDNRLVGIITAADIVKYLAKNKNIGKVLDAGTKNPITINRYYSILNAAKLMIEKRIGTLLVMENQKLVGIVTERDLMYAYINIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VB6</accession>
    <name>Q97VB6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-carbamoyl-L-amino acid amidohydrolase (AmaB)</fullName>
            <ecNumber evidence="2">3.5.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">amaB</name>
        <name evidence="2" type="ordered locus">SSO2719</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.-" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90446">
        <property type="entry name" value="F90446"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988650.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VB6"/>
    <dbReference type="FunCoup" id="Q97VB6">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2719"/>
    <dbReference type="PaxDb" id="273057-SSO2719"/>
    <dbReference type="EnsemblBacteria" id="AAK42829">
        <property type="protein sequence ID" value="AAK42829"/>
        <property type="gene ID" value="SSO2719"/>
    </dbReference>
    <dbReference type="GeneID" id="1452748"/>
    <dbReference type="KEGG" id="sso:SSO2719"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2800"/>
    <dbReference type="eggNOG" id="arCOG01109">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024588_6_0_2"/>
    <dbReference type="InParanoid" id="Q97VB6"/>
    <dbReference type="PhylomeDB" id="Q97VB6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047652">
        <property type="term" value="F:allantoate deiminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000256">
        <property type="term" value="P:allantoin catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03884">
        <property type="entry name" value="M20_bAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010158">
        <property type="entry name" value="Amidase_Cbmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01879">
        <property type="entry name" value="hydantase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006771">
        <property type="entry name" value="PRK09290.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494:SF5">
        <property type="entry name" value="ALLANTOATE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494">
        <property type="entry name" value="ALLANTOATE DEIMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001235">
        <property type="entry name" value="Amidase_carbamoylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="401" mass="45164" checksum="F5895865B81B6468" modified="2001-10-01" version="1">MNPERFLTTFHSLTNIGWTEDGVLRLALNEYDIKVREELIKILSSIGVHIMVDDAGNIIGELGGKLSDAIAIGSHMDSVPYGGKYDGFYGVMAGLEVLRSIKERGISNHSIKLIDFTNEEGSRFQPSLLGSGLTTGIFDKNYVYSRRDKDNISFEEALRVSGFMGDESNRLMHMKPNYYLELHIEQGPILEEEGYQIGIPLGIAGLSVYEFTFKGQSSQTGPTPMDRRRDALVGASKFVVSVRDHAKKQENLRATVGILNVKPNVYNAIPREVRLTVDVRSIERNRIDHTINEFVNIAKSIADDEKLEVEYRHLWTANPVSFSDEVISVIERACKELSMRYKFMYSWAGHDAQYMTKISKVGMIFIPSHLGISHAKEEYSSDEDMLNGLRVLEKAVELLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="141" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VH4</accession>
    <name>Q97VH4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cation transporting ATPase (PacS)</fullName>
            <ecNumber evidence="12">3.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pacS</name>
        <name evidence="12" type="ordered locus">SSO2651</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="12"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90439">
        <property type="entry name" value="C90439"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VH4"/>
    <dbReference type="FunCoup" id="Q97VH4">
        <property type="interactions" value="120"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2651"/>
    <dbReference type="TCDB" id="3.A.3.5.24">
        <property type="family name" value="the p-type atpase (p-atpase) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2651"/>
    <dbReference type="EnsemblBacteria" id="AAK42770">
        <property type="protein sequence ID" value="AAK42770"/>
        <property type="gene ID" value="SSO2651"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2651"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2732"/>
    <dbReference type="eggNOG" id="arCOG01576">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_0_3_2"/>
    <dbReference type="InParanoid" id="Q97VH4"/>
    <dbReference type="PhylomeDB" id="Q97VH4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000111">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01511">
        <property type="entry name" value="ATPase-IB1_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01512">
        <property type="entry name" value="ATPase-IB2_Cd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF8">
        <property type="entry name" value="P-TYPE CU(+) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00943">
        <property type="entry name" value="CUATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="417"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="707"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="735"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="11">
        <location>
            <begin position="21"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK42770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="755" mass="83008" checksum="AD4AFCF60D5880DA" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VJ6</accession>
    <name>Q97VJ6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CoA-ligase / coenzyme F390 synthetase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2627</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90436">
        <property type="entry name" value="E90436"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VJ6"/>
    <dbReference type="STRING" id="273057.SSO2627"/>
    <dbReference type="PaxDb" id="273057-SSO2627"/>
    <dbReference type="EnsemblBacteria" id="AAK42748">
        <property type="protein sequence ID" value="AAK42748"/>
        <property type="gene ID" value="SSO2627"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2627"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2710"/>
    <dbReference type="eggNOG" id="arCOG02620">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035301_1_1_2"/>
    <dbReference type="InParanoid" id="Q97VJ6"/>
    <dbReference type="PhylomeDB" id="Q97VJ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028154">
        <property type="entry name" value="AMP-dep_Lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43845">
        <property type="entry name" value="BLR5969 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43845:SF1">
        <property type="entry name" value="BLR5969 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14535">
        <property type="entry name" value="AMP-binding_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent ligase C-terminal" evidence="2">
        <location>
            <begin position="252"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="339" mass="38054" checksum="3B96851F2613C69D" modified="2001-10-01" version="1">MTSGTTGVPTVNAYTWSDIEIWTSLVARSLVTAGVTKNDIVMNIYGYGLFTGGIGLHQGIQRIGAKVIPWSTGRTEALARALKDFKATVITGTPSYELLIAETLRKLNIDAEKELQLRLAIPGAEAMSKEMLERIEHELGLKAREGRALEIYGLTEALGPGVAQECPDDNHEWLHIWTDHYLVEIIDPETGERISEDEEGEMVITTLSKEGMPLIRYRTRDITRLIESDDEIPFPKIGMLKGRVDDVIFYKGVKVFPTAISNVLMSCEEVKEFQIVVDKTNREHRLIVRVETEKPSEKLAEKLIEEIRTVAFVTPEVEFVSLGTLPRFEGKSKRVVIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VL2</accession>
    <name>Q97VL2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4382 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2611</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90434">
        <property type="entry name" value="E90434"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989331.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VL2"/>
    <dbReference type="STRING" id="273057.SSO2611"/>
    <dbReference type="PaxDb" id="273057-SSO2611"/>
    <dbReference type="EnsemblBacteria" id="AAK42732">
        <property type="protein sequence ID" value="AAK42732"/>
        <property type="gene ID" value="SSO2611"/>
    </dbReference>
    <dbReference type="GeneID" id="1454059"/>
    <dbReference type="KEGG" id="sso:SSO2611"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2692"/>
    <dbReference type="eggNOG" id="arCOG06011">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1599091_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VL2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025491">
        <property type="entry name" value="DUF4382"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14321">
        <property type="entry name" value="DUF4382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4382" evidence="2">
        <location>
            <begin position="28"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="166" mass="17914" checksum="7C6DBA03491912BE" modified="2001-10-01" version="1">MNKAILGIVIVVLVLAGGVYYGFYYLTTGVVNVYIQDPPTSQGVKIYLTISSIMIHKVNASNDSWITISNKTITILLTSNMTFLASSRIPSGQYNEVFLEISFAQVQLGNVNISAKIPSGVFKIHIINGMDLKGGSSQSLLISFPHITYANGQIIISPSITAEVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VQ6</accession>
    <name>Q97VQ6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1234</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90428">
        <property type="entry name" value="G90428"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923922.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VQ6"/>
    <dbReference type="PaxDb" id="273057-SSO2557"/>
    <dbReference type="EnsemblBacteria" id="AAK42686">
        <property type="protein sequence ID" value="AAK42686"/>
        <property type="gene ID" value="SSO2557"/>
    </dbReference>
    <dbReference type="GeneID" id="1454013"/>
    <dbReference type="KEGG" id="sso:SSO2557"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2639"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075479_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VQ6"/>
    <dbReference type="PhylomeDB" id="Q97VQ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="319" mass="36828" checksum="A8B00459A66EF85E" modified="2001-10-01" version="1">MGVEGSTMARTLRHIPNLMYYPLPPIEDMPWREKWLTEIKPVLDAMDLERVLGEGALVYLKLLIVMVLYSCSYRDAVKMVNVNVVVAWFVGRKVGKSTLHDFVGRLYGVRKKLLEISFKLEEKCLPSYLPASAHLVDFMAWLVDSFLLDLPPGKRSVETFREKAELERREGNLERARKLLSLGRTKRRFEGRWTKKRGVSHYGLKAVAVISVSLFVRSITLKPANFSDKRFKSPLKGIKIADRGFSPSPTQLIAREKPFTTLRAHVEFFGTYLNAFWRPYGTTTWRNDVFLHVLGVIYNIKIFLAIQRRTPPERRAVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VS2</accession>
    <name>Q97VS2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90426">
        <property type="entry name" value="F90426"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923911.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VS2"/>
    <dbReference type="PaxDb" id="273057-SSO2540"/>
    <dbReference type="EnsemblBacteria" id="AAK42669">
        <property type="protein sequence ID" value="AAK42669"/>
        <property type="gene ID" value="SSO2540"/>
    </dbReference>
    <dbReference type="GeneID" id="1453996"/>
    <dbReference type="KEGG" id="sso:SSO2540"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2617"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VS2"/>
    <dbReference type="PhylomeDB" id="Q97VS2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="80"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37383" checksum="D2C566F4F39A41AC" modified="2001-10-01" version="1">MQTIQVSKTELKSLAINLATININVISQDLDPEIVKAAPSLLTGNRGKYYLKVVRRGEKVISKGQRTFKFYPIYREVKGEINVVAVDETGLTVGEKEQEKAEGFLLYNWKRKGVKMRSLDLVYPLRLPLLVEVADLRSDSPSQFLLRSVREVSQYMEIDYVVADAGFLNLGVIKEMPVKTIVRGKSNLKGFKELSNVPLVEKRYEVKDKVYVAYRVLEFEGLYYYDVVYVKGKPRHFMFVTNFEGDPYELAELYRLRWQVEEGFKVRKARIRYVRKLSNKIFLFLYYTVLDSAWNLVNHLLFNFKSTCKKVLSFDSFVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VT6</accession>
    <name>Q97VT6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Long-chain-fatty-acid--CoA ligase (FadD-2)</fullName>
            <ecNumber evidence="3">6.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fadD-2</name>
        <name evidence="3" type="ordered locus">SSO2523</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90424">
        <property type="entry name" value="G90424"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988474.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VT6"/>
    <dbReference type="FunCoup" id="Q97VT6">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2523"/>
    <dbReference type="PaxDb" id="273057-SSO2523"/>
    <dbReference type="EnsemblBacteria" id="AAK42654">
        <property type="protein sequence ID" value="AAK42654"/>
        <property type="gene ID" value="SSO2523"/>
    </dbReference>
    <dbReference type="GeneID" id="1453981"/>
    <dbReference type="KEGG" id="sso:SSO2523"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2600"/>
    <dbReference type="eggNOG" id="arCOG00856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_9_2"/>
    <dbReference type="InParanoid" id="Q97VT6"/>
    <dbReference type="PhylomeDB" id="Q97VT6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05935">
        <property type="entry name" value="LC_FACS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050237">
        <property type="entry name" value="ATP-dep_AMP-bd_enzyme"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006181">
        <property type="entry name" value="PRK08314.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767:SF1">
        <property type="entry name" value="NONRIBOSOMAL PEPTIDE SYNTHASE PES1 (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="1">
        <location>
            <begin position="35"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="2">
        <location>
            <begin position="467"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="559" mass="63308" checksum="215A330878F303F4" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VV1</accession>
    <name>Q97VV1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2506</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90422">
        <property type="entry name" value="H90422"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VV1"/>
    <dbReference type="SMR" id="Q97VV1"/>
    <dbReference type="STRING" id="273057.SSO2506"/>
    <dbReference type="PaxDb" id="273057-SSO2506"/>
    <dbReference type="EnsemblBacteria" id="AAK42639">
        <property type="protein sequence ID" value="AAK42639"/>
        <property type="gene ID" value="SSO2506"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2506"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2585"/>
    <dbReference type="eggNOG" id="arCOG02647">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_12_6_2"/>
    <dbReference type="InParanoid" id="Q97VV1"/>
    <dbReference type="PhylomeDB" id="Q97VV1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.10">
        <property type="entry name" value="Tetracycline Repressor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050624">
        <property type="entry name" value="HTH-type_Tx_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43479">
        <property type="entry name" value="ACREF/ENVCD OPERON REPRESSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43479:SF11">
        <property type="entry name" value="ACREF_ENVCD OPERON REPRESSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="Tetracyclin repressor-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="14"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="2">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="198" mass="23506" checksum="F0DC16E11C0AB081" modified="2001-10-01" version="1">MVQMYRYPKTEKGKESLNKIINASLDLISEKGFLNTSISDITNKAGVAYGLFYFYFKSKHDILDELIRKFNTDMRYYLKVNTMGIRNRIEMEKEGLAKFLEWMSQNKKYYKVFVEAQVHRPEMYIWHFTKLAERYKIGLKEAMDRGEIVKVDPELLAYVLIGIGEMIGRRYILWTNQGLTKKQYNDLAILIENMLRPK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2025-06-18" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W36</accession>
    <name>LEU1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>2-isopropylmalate synthase</fullName>
            <shortName>IPMS</shortName>
            <ecNumber evidence="2">2.3.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Alpha-isopropylmalate synthase</fullName>
            <shortName>Alpha-IPM synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">leuA</name>
        <name type="ordered locus">SSO2407</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate). Carries out the first step of the leucine biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21524"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:21525"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="frameshift">
            <sequence resource="EMBL-CDS" id="AAK42554" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42554.1"/>
        <property type="status" value="ALT_FRAME"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90412">
        <property type="entry name" value="C90412"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W36"/>
    <dbReference type="SMR" id="Q97W36"/>
    <dbReference type="FunCoup" id="Q97W36">
        <property type="interactions" value="265"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2407"/>
    <dbReference type="PaxDb" id="273057-SSO2407"/>
    <dbReference type="EnsemblBacteria" id="AAK42554">
        <property type="protein sequence ID" value="AAK42554"/>
        <property type="gene ID" value="SSO2407"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2407"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2489"/>
    <dbReference type="eggNOG" id="arCOG02092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_4_2_2"/>
    <dbReference type="InParanoid" id="Q97W36"/>
    <dbReference type="PhylomeDB" id="Q97W36"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00070"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07940">
        <property type="entry name" value="DRE_TIM_IPMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.260:FF:000001">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000010">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050073">
        <property type="entry name" value="2-IPM_HCS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054948">
        <property type="entry name" value="IPMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011830">
        <property type="entry name" value="LEU1_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041069">
        <property type="entry name" value="IPMS_Sufob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02090">
        <property type="entry name" value="LEU1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277:SF9">
        <property type="entry name" value="2-ISOPROPYLMALATE SYNTHASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277">
        <property type="entry name" value="HOMOCITRATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000140424" description="2-isopropylmalate synthase">
        <location>
            <begin position="1"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="4">
        <location>
            <begin position="15"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P9WQB3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q4JA78"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q9JZG1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="386" mass="43013" checksum="61D59B56AA2EC717" modified="2023-02-22" version="2">MVRTRYEYSLRSKKIRIFDTTLRDGEQAPGIDLTTEQKIMIARKLADLGVDVIEAGFPASSEGEFIATRKIFEEIGDQVEVIGLSRSNKNDIDKTISTGISSIHLFIATSELHMKYKLKMTKEEVLNKIYESVKYAKDHGMIVEFSPEDATRTEEDFLFTAIRTAIEAGADRINIPDTVGTMHPFKYYDMIKKIVNFVGERIIVSVHCHNDFGLATANSLAGVYAGARQAHVTVNGIGERAGNASLEEVVMGIKKLLNYETNVKTWKLYEVSRFVAEMTGVPVPYFKAIVGDNAFGHESGIHVHGVIENPFTYEPISPEEVGNFRRLALGKHSGIHGLRKLLEEQGIYLSDDKLKIVLAEIKKLAESGNKVTVEDAKNIALKLMNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W80</accession>
    <name>Q97W80_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphatase, putative (NagD-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nagD-like</name>
        <name evidence="1" type="ordered locus">SSO2355</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90406">
        <property type="entry name" value="E90406"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989497.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W80"/>
    <dbReference type="FunCoup" id="Q97W80">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2355"/>
    <dbReference type="PaxDb" id="273057-SSO2355"/>
    <dbReference type="EnsemblBacteria" id="AAK42508">
        <property type="protein sequence ID" value="AAK42508"/>
        <property type="gene ID" value="SSO2355"/>
    </dbReference>
    <dbReference type="GeneID" id="1453835"/>
    <dbReference type="KEGG" id="sso:SSO2355"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2442"/>
    <dbReference type="eggNOG" id="arCOG04221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043473_1_2_2"/>
    <dbReference type="InParanoid" id="Q97W80"/>
    <dbReference type="PhylomeDB" id="Q97W80"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF46">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="264" mass="29032" checksum="355CA4FB701A64D9" modified="2001-10-01" version="1">MSVLNGYQLIISDVDGVIVREGDPIWENIQALRNIQNNGVKIIFVTNNSGFSRILLSRQLSYLGLKVTPDMIITSGLAAAIYMKEKLNVKSVFAVGEEGLIEELKNHGFLVFSSAESERILPDAVVMGLDRLSTYDKLSLAMRCISKGSKFIVTNMDRLWPAKDGLKLGAGALASSIIYALRRDPDFIAGKPNTWIVEIAMRISNVKKLDKILVIGDQIETDIQMGYNIGADTALVLTGISNVDDVDRSNVKPKYVVNTLLDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W82</accession>
    <name>Q97W82_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2070 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2354</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90406">
        <property type="entry name" value="C90406"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989499.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W82"/>
    <dbReference type="STRING" id="273057.SSO2354"/>
    <dbReference type="PaxDb" id="273057-SSO2354"/>
    <dbReference type="EnsemblBacteria" id="AAK42506">
        <property type="protein sequence ID" value="AAK42506"/>
        <property type="gene ID" value="SSO2354"/>
    </dbReference>
    <dbReference type="GeneID" id="1453833"/>
    <dbReference type="KEGG" id="sso:SSO2354"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2440"/>
    <dbReference type="eggNOG" id="arCOG04351">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_511562_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W82"/>
    <dbReference type="PhylomeDB" id="Q97W82"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019204">
        <property type="entry name" value="DUF2070_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09843">
        <property type="entry name" value="DUF2070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="515"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2070" evidence="2">
        <location>
            <begin position="10"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="538" mass="61809" checksum="15133EF7992D9955" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WA5</accession>
    <name>Q97WA5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1058</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO10228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90403">
        <property type="entry name" value="D90403"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WA5"/>
    <dbReference type="PaxDb" id="273057-SSO10228"/>
    <dbReference type="EnsemblBacteria" id="AAK42483">
        <property type="protein sequence ID" value="AAK42483"/>
        <property type="gene ID" value="SSO10228"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO10228"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2417"/>
    <dbReference type="HOGENOM" id="CLU_3178661_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WA5"/>
    <dbReference type="PhylomeDB" id="Q97WA5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053172">
        <property type="entry name" value="Tn903_transposase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631:SF3">
        <property type="entry name" value="ISSOD12 TRANSPOSASE TNPA_ISSOD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="50" mass="6354" checksum="0EDF90C4DFD27185" modified="2001-10-01" version="1">MGKSKYKRDWSKYDENVITRYKLMFPFYVFEHWWDLLAEENRNARTKYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WB5</accession>
    <name>Q97WB5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Conserved flagellar protein F immunoglobulin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2319</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90402">
        <property type="entry name" value="B90402"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992493.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WB5"/>
    <dbReference type="STRING" id="273057.SSO2319"/>
    <dbReference type="PaxDb" id="273057-SSO2319"/>
    <dbReference type="EnsemblBacteria" id="AAK42473">
        <property type="protein sequence ID" value="AAK42473"/>
        <property type="gene ID" value="SSO2319"/>
    </dbReference>
    <dbReference type="GeneID" id="1453800"/>
    <dbReference type="KEGG" id="sso:SSO2319"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2407"/>
    <dbReference type="eggNOG" id="arCOG01824">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631727_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WB5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054458">
        <property type="entry name" value="FlaF_Ig-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22201">
        <property type="entry name" value="FlaF_Ig-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved flagellar protein F immunoglobulin-like" evidence="2">
        <location>
            <begin position="49"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="162" mass="18148" checksum="3A54DEB5A424B684" modified="2001-10-01" version="1">MGVSQVVAYVLIFFITISLGLIALEAYIKSQQLLLHAENLRQNMELNQLNTRIFIKSIAINGNLLYITITNNGSTALFDFRDFAIIIKYYANISNISTLIISNYNYSTNLGPYKWVSNTVLINPNMVGTFIADLPYPPYPNTMATVVVASNYGPEAIWRGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WC5</accession>
    <name>Q97WC5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cobalamin biosynthesis precorrin-3 methylase, putative (CbiH)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cbiH</name>
        <name evidence="7" type="ordered locus">SSO2306</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="H90400"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WC5"/>
    <dbReference type="FunCoup" id="Q97WC5">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2306"/>
    <dbReference type="PaxDb" id="273057-SSO2306"/>
    <dbReference type="EnsemblBacteria" id="AAK42463">
        <property type="protein sequence ID" value="AAK42463"/>
        <property type="gene ID" value="SSO2306"/>
    </dbReference>
    <dbReference type="GeneID" id="1453790"/>
    <dbReference type="KEGG" id="sso:SSO2306"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2395"/>
    <dbReference type="eggNOG" id="arCOG00647">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047948_2_0_2"/>
    <dbReference type="InParanoid" id="Q97WC5"/>
    <dbReference type="PhylomeDB" id="Q97WC5"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11646">
        <property type="entry name" value="Precorrin_3B_C17_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006363">
        <property type="entry name" value="Cbl_synth_CobJ/CibH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051810">
        <property type="entry name" value="Precorrin_MeTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01466">
        <property type="entry name" value="cobJ_cbiH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004423">
        <property type="entry name" value="PRK05765.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47036">
        <property type="entry name" value="COBALT-FACTOR III C(17)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47036:SF1">
        <property type="entry name" value="COBALT-FACTOR III C(17)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="6">
        <location>
            <begin position="5"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="250" mass="27981" checksum="870090C6123D4E22" modified="2001-10-01" version="1">MKMGRLYIVGIGPGSKEQRTIKAQEVLEKSNVIIGYNTYLRLISDVLDGKKEVIGARMKEEIFRANTAIEKALESNNTVALVSSGDPQVYGMAGLVYDLIARRNLDLDVEVVPGVTAALAAAARLGSPLSLDFVVISLSDLLIPREEILHKVTKAAEADFVIVFYNLINENLLIEVMDIISKHRKPNTPVGLVKSAYRNNENIIITTLSSWKEYMNEISMSTTMIIGNSLTYRYKNYMITPRGYERKYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WG1</accession>
    <name>Q97WG1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sulfide-quinone reductase related protein (Sqr)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">sqr</name>
        <name evidence="2" type="ordered locus">SSO2261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90396">
        <property type="entry name" value="C90396"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991528.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WG1"/>
    <dbReference type="STRING" id="273057.SSO2261"/>
    <dbReference type="PaxDb" id="273057-SSO2261"/>
    <dbReference type="DNASU" id="1453753"/>
    <dbReference type="EnsemblBacteria" id="AAK42426">
        <property type="protein sequence ID" value="AAK42426"/>
        <property type="gene ID" value="SSO2261"/>
    </dbReference>
    <dbReference type="GeneID" id="1453753"/>
    <dbReference type="KEGG" id="sso:SSO2261"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2353"/>
    <dbReference type="eggNOG" id="arCOG01065">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030742_1_1_2"/>
    <dbReference type="InParanoid" id="Q97WG1"/>
    <dbReference type="PhylomeDB" id="Q97WG1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052541">
        <property type="entry name" value="SQRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755:SF1">
        <property type="entry name" value="FAD-DEPENDENT PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="8"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="207"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="414" mass="45826" checksum="354B5D4AD9E16A6F" modified="2001-10-01" version="1">MRLRSMTKVLVLGARFGGLSAAYALKRLAGNKAEIKVINNTRFSYFRPALPHVATGVINENDVMVDLTKALPEKRINFQQGTVEKIDASGGLVYYTKPDGDKAEEDYDYLIIALGAHLGTELVKGWDKYGYSVCELDYALKLREKLKDFKGGTIAIGSGLFYQGKSPKPKVPENYVPVADAACEGPIFEMSLMLSGYFKKKGMLNKVKFVVFSPGEYLSDLSTMSRKIVREMYKQMGIELVDNFRIKEIRENEIADEAGKTIKADLTILIPPYTGNPALKNSTPDLIDDGGFVPTDLNMVSINYDNVYAVGDANSLTVPKLGYLAVKTGNIAAQHLATRLGVQVKVDQYYPTIICVADNPFEGFGIAVKDDTWYGGTTSIAAPSPINHIKKELFHKYFIWTKGDMALEKFLASW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WH8</accession>
    <name>Q97WH8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BPS2 protein homolog (Bps2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bps2</name>
        <name evidence="3" type="ordered locus">SSO2241</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90394">
        <property type="entry name" value="A90394"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992029.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WH8"/>
    <dbReference type="SMR" id="Q97WH8"/>
    <dbReference type="STRING" id="273057.SSO2241"/>
    <dbReference type="PaxDb" id="273057-SSO2241"/>
    <dbReference type="EnsemblBacteria" id="AAK42408">
        <property type="protein sequence ID" value="AAK42408"/>
        <property type="gene ID" value="SSO2241"/>
    </dbReference>
    <dbReference type="GeneID" id="1453735"/>
    <dbReference type="KEGG" id="sso:SSO2241"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2335"/>
    <dbReference type="eggNOG" id="arCOG00373">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_468999_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WH8"/>
    <dbReference type="PhylomeDB" id="Q97WH8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.510">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045487">
        <property type="entry name" value="ASRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114:SF2">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="130"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="323"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="587" mass="68437" checksum="B90DBC1E19C05E86" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WJ3</accession>
    <name>Q97WJ3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF371 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2224</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90392">
        <property type="entry name" value="B90392"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992056.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WJ3"/>
    <dbReference type="STRING" id="273057.SSO2224"/>
    <dbReference type="PaxDb" id="273057-SSO2224"/>
    <dbReference type="EnsemblBacteria" id="AAK42393">
        <property type="protein sequence ID" value="AAK42393"/>
        <property type="gene ID" value="SSO2224"/>
    </dbReference>
    <dbReference type="GeneID" id="1453720"/>
    <dbReference type="KEGG" id="sso:SSO2224"/>
    <dbReference type="eggNOG" id="arCOG04171">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135994_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WJ3"/>
    <dbReference type="PhylomeDB" id="Q97WJ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.630">
        <property type="entry name" value="mth639 domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007171">
        <property type="entry name" value="DUF371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023131">
        <property type="entry name" value="Mth639-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40696:SF1">
        <property type="entry name" value="DUF371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40696">
        <property type="entry name" value="DUF371 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04027">
        <property type="entry name" value="DUF371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18254" checksum="C964E63F7E1492C7" modified="2001-10-01" version="1">MIAIDKVKIKGHHNVRAFHKTTLEITKDDYLTSRGDCIIGILANKGARDLSEDLKKIARNDKSFIYGVIRVKEFLDVVHGRGSSKFTFENQNKIIFRKSTFIEGSTVMINSDKAARDINREIIDLLKTEIEGEIIILASDQPLEDKEILRIVVNLNPRSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WK4</accession>
    <name>Q97WK4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90390">
        <property type="entry name" value="G90390"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WK4"/>
    <dbReference type="STRING" id="273057.SSO2212"/>
    <dbReference type="PaxDb" id="273057-SSO2212"/>
    <dbReference type="EnsemblBacteria" id="AAK42382">
        <property type="protein sequence ID" value="AAK42382"/>
        <property type="gene ID" value="SSO2212"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2212"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2308"/>
    <dbReference type="eggNOG" id="arCOG07315">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1178149_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WK4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="256" mass="29374" checksum="4BB17893AD2B7D2C" modified="2001-10-01" version="1">MICTTIQYLIQLRSYLKNSLCTIYYMSVKFEESSRLLEAIKSMLASATSSILIVTYGIDQEAASEILTKAASGVNTVLVTADKDWARWLKNQAEAYKKDEEKRLYRELRRNETIYTQIIYFTLIISIAIAIIDIILYFMMGTLVYYDVPLSIIAIGFLIYYGIRRRKEALSQISILRESAQNFVQEVGQIRQKIKEKLKIIEIDNQVSFSIVSCDDKTILFSAPLKFSMEKSSVHVVMEISKQLADQLVNLILKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WM5</accession>
    <name>Q97WM5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2188</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90388">
        <property type="entry name" value="B90388"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992109.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WM5"/>
    <dbReference type="STRING" id="273057.SSO2188"/>
    <dbReference type="PaxDb" id="273057-SSO2188"/>
    <dbReference type="EnsemblBacteria" id="AAK42361">
        <property type="protein sequence ID" value="AAK42361"/>
        <property type="gene ID" value="SSO2188"/>
    </dbReference>
    <dbReference type="GeneID" id="1453688"/>
    <dbReference type="KEGG" id="sso:SSO2188"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2284"/>
    <dbReference type="eggNOG" id="arCOG05489">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1412424_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WM5"/>
    <dbReference type="PhylomeDB" id="Q97WM5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="169" mass="19903" checksum="89A4FC2A622B6996" modified="2001-10-01" version="1">MSENVTPIFGLGNFVMDKLGNVTQYTFFYYYDPENYYVSLPREMLDKEMELVKENMQSFLDEEKIIINDRVTRAKVVSTDIFLLDTTHPVIEFIITFKGTLKEGLNVYENFYEEEITEYPYEAIWRFPGKILNVKMKGQVSILENFLKIKVNKGIKVGGRERIEFFLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WN2</accession>
    <name>Q97WN2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90387">
        <property type="entry name" value="C90387"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992120.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WN2"/>
    <dbReference type="PaxDb" id="273057-SSO2179"/>
    <dbReference type="EnsemblBacteria" id="AAK42354">
        <property type="protein sequence ID" value="AAK42354"/>
        <property type="gene ID" value="SSO2179"/>
    </dbReference>
    <dbReference type="GeneID" id="1453681"/>
    <dbReference type="KEGG" id="sso:SSO2179"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2275"/>
    <dbReference type="eggNOG" id="arCOG06040">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1068006_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WN2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="265" mass="31527" checksum="F8D949DA90F5B362" modified="2001-10-01" version="1">MNFITFAEKLGIDREAAIKVYRLFNGGYFESLYYSKPPILHKLREWPRKYLTKKLILIKNFQLNQAFEALIWADIIAIYGMSSKLIDRPLKYGILEKNIEYIYEEIKKYSLSNNFTDYPTTLSLDFIKVDFSPFIKDLTNKRMEEMKANDSEIINDIAYDSKLMEEIKIKYPWAKNVKRENAVRAFQLSERVNEFVEYIIPFIYYLAASKTLHFDYTLLSNTISDTIKLVEEEGSRAIKEQEMSSEYQRKVRELYQLIITTLNYF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-10-11" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WP0</accession>
    <name>NTPTH_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoside-triphosphatase THEP1</fullName>
            <shortName evidence="1">NTPase THEP1</shortName>
            <ecNumber evidence="1">3.6.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside triphosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2171</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Has nucleotide phosphatase activity towards ATP, GTP, CTP, TTP and UTP. May hydrolyze nucleoside diphosphates with lower efficiency.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="EC" id="3.6.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the THEP1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.15" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90386">
        <property type="entry name" value="C90386"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992133.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WP0"/>
    <dbReference type="SMR" id="Q97WP0"/>
    <dbReference type="FunCoup" id="Q97WP0">
        <property type="interactions" value="148"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2171"/>
    <dbReference type="PaxDb" id="273057-SSO2171"/>
    <dbReference type="EnsemblBacteria" id="AAK42346">
        <property type="protein sequence ID" value="AAK42346"/>
        <property type="gene ID" value="SSO2171"/>
    </dbReference>
    <dbReference type="GeneID" id="7805661"/>
    <dbReference type="KEGG" id="sso:SSO2171"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2266"/>
    <dbReference type="eggNOG" id="arCOG01034">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103145_1_1_2"/>
    <dbReference type="InParanoid" id="Q97WP0"/>
    <dbReference type="PhylomeDB" id="Q97WP0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19482">
        <property type="entry name" value="RecA-like_Thep1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00796">
        <property type="entry name" value="NTPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004948">
        <property type="entry name" value="Nuc-triphosphatase_THEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010248">
        <property type="entry name" value="PRK13695.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43146">
        <property type="entry name" value="CANCER-RELATED NUCLEOSIDE-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43146:SF1">
        <property type="entry name" value="CANCER-RELATED NUCLEOSIDE-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03266">
        <property type="entry name" value="NTPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000146705" description="Nucleoside-triphosphatase THEP1">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="15"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="106"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00796"/>
        </source>
    </evidence>
    <sequence length="175" mass="19730" checksum="6E2A814F2E8FEFCC" modified="2001-10-01" version="1">MLEESKNALRVFITGNPGVGKTTILLFLINKLSENNYKVAGFYCPEVRENGRRIGFRIVDITTNEGDWLAKENAPGRVKIGKYTVLEDSAKRITEITLSNINKADVLAIDEIGPMELKIPTIKKLIETILNNQKPLIAVLHRTQKPMGGRIYVITVENRDSIKYEILNYILSSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WQ8</accession>
    <name>Q97WQ8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase (Acd-3)</fullName>
            <ecNumber evidence="10">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">acd-3</name>
        <name evidence="10" type="ordered locus">SSO2060</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="10"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90373">
        <property type="entry name" value="E90373"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988843.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WQ8"/>
    <dbReference type="STRING" id="273057.SSO2060"/>
    <dbReference type="PaxDb" id="273057-SSO2060"/>
    <dbReference type="EnsemblBacteria" id="AAK42244">
        <property type="protein sequence ID" value="AAK42244"/>
        <property type="gene ID" value="SSO2060"/>
    </dbReference>
    <dbReference type="GeneID" id="1453571"/>
    <dbReference type="KEGG" id="sso:SSO2060"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2139"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_2"/>
    <dbReference type="InParanoid" id="Q97WQ8"/>
    <dbReference type="PhylomeDB" id="Q97WQ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00567">
        <property type="entry name" value="ACAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="3"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="111"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="214"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="371" mass="41395" checksum="A1B2C43EEA106884" modified="2001-10-01" version="1">MSEKELFIQSLREFLNRDVEKIVNKIDKEDYYPRDLVRKLGELGFLIPLTSGLSNYDMMIILEEIAKVSGSLALIADAQGELAGEMIRLYANERQKKEFMEPLSKGEIIGSFALTEPSGGSDIGSMKTLAEKRNGSWIIKGHKMWITQGLYADVFITVAKTGSSRRDLSVFIVPRSNCVETRKIEVMGNRGTGTAEVIFHNCEVSNDYVVGEVNNGWNMVNSVLEIGRLAISGIAIGLAEAALEEAFKWAKSRFAFGNSIYDFQGIRWYFADAIAKLNAVKSMAKDVSSKFDENSKDKGVYVSMLKLLSANIANEVIDISLQVFGGMGYAKGTNIERIYRDARLLRIGEGTDEVQRHIIAKYIEQYGIPYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WS8</accession>
    <name>Q97WS8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Membrane-bound metal-dependent hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99371">
        <property type="entry name" value="A99371"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923744.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WS8"/>
    <dbReference type="PaxDb" id="273057-SSO2038"/>
    <dbReference type="EnsemblBacteria" id="AAK42224">
        <property type="protein sequence ID" value="AAK42224"/>
        <property type="gene ID" value="SSO2038"/>
    </dbReference>
    <dbReference type="GeneID" id="1453551"/>
    <dbReference type="KEGG" id="sso:SSO2038"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2119"/>
    <dbReference type="eggNOG" id="arCOG07202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_965116_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WS8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="34051" checksum="A9C342F962E7C587" modified="2001-10-01" version="1">MNLHTHIILALAFGLIFFHNDITLAVLVGIGAAIPDLDREYVLTKRKIFAKYQLHRALFHNIFFALAMVYLNFYLGLGIFLHMALDLLTSPTDRGVELFFPLGRLVKNYELDYDGNIRKSRGIMWYLEDPMRIINKTADPGLKEVVKMPWIRIYGPFKNSRLVDWMIFYSSFVFIQLYELNHLAEWWKIFLYTVFVKFTFIVIGIVLFYFTGELWRRRLQFQNLNSKLKYVILGVMALGLSLILFQGINLYSPMKPIINFNTLVLIIVSMLVGLFLAYIHVRLRFKKVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WT7</accession>
    <name>Q97WT7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2027</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90369">
        <property type="entry name" value="G90369"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923735.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WT7"/>
    <dbReference type="FunCoup" id="Q97WT7">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2027"/>
    <dbReference type="PaxDb" id="273057-SSO2027"/>
    <dbReference type="EnsemblBacteria" id="AAK42214">
        <property type="protein sequence ID" value="AAK42214"/>
        <property type="gene ID" value="SSO2027"/>
    </dbReference>
    <dbReference type="GeneID" id="7797414"/>
    <dbReference type="KEGG" id="sso:SSO2027"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2107"/>
    <dbReference type="eggNOG" id="arCOG02703">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129117_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WT7"/>
    <dbReference type="PhylomeDB" id="Q97WT7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861:SF3">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_2G14390)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="2">
        <location>
            <begin position="46"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="189" mass="21751" checksum="F9F6DB9082E19C3F" modified="2001-10-01" version="1">MYTLMSKNDRYINPLVLDNSLRSLVSPPNRILSRFVKYLKEDYTVVDLGCGPGFFTTILARIVKTVYAVDPDERAIRRLKEKVQKLSLNNVIPYVAPAQKLEFIKDKSIDFVFSNLMLCCTSDHNGAIKEIKRILKDNGLAYISVTRSFLIKDKMDVSGDEWKKILGQFKIIREGKNLMERWAVVQQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WV2</accession>
    <name>Q97WV2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PaREP1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90367">
        <property type="entry name" value="G90367"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WV2"/>
    <dbReference type="SMR" id="Q97WV2"/>
    <dbReference type="STRING" id="273057.SSO2012"/>
    <dbReference type="PaxDb" id="273057-SSO2012"/>
    <dbReference type="DNASU" id="1453525"/>
    <dbReference type="EnsemblBacteria" id="AAK42198">
        <property type="protein sequence ID" value="AAK42198"/>
        <property type="gene ID" value="SSO2012"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2012"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2085"/>
    <dbReference type="eggNOG" id="arCOG03721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115256_2_0_2"/>
    <dbReference type="InParanoid" id="Q97WV2"/>
    <dbReference type="PhylomeDB" id="Q97WV2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="18718" checksum="14ABF070834D0EE8" modified="2001-10-01" version="1">MKKFPELRKYTNPVWVRLVILCQKKLKVCFIMFIVMSISTSAEIYYEEAEEFLSKGDLVQACEKYYKAAEEAIKLLVIENNLKEIINEAEEHGWDSKTLNDAVTELSYKLGDNIIDMWASAVTLFTARKFMDKDLIENYKKDIKTLIEEAKQRFSIKVLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2024-07-24" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WW9</accession>
    <name>CMR3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR system CMR subunit Cmr3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>CRISPR type III-B/RAMP module-associated protein Cmr3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cmr3</name>
        <name type="ordered locus">SSO1992</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="Mol. Cell" volume="45" first="303" last="313">
            <title>Structure and mechanism of the CMR complex for CRISPR-mediated antiviral immunity.</title>
            <authorList>
                <person name="Zhang J."/>
                <person name="Rouillon C."/>
                <person name="Kerou M."/>
                <person name="Reeks J."/>
                <person name="Brugger K."/>
                <person name="Graham S."/>
                <person name="Reimann J."/>
                <person name="Cannone G."/>
                <person name="Liu H."/>
                <person name="Albers S.V."/>
                <person name="Naismith J.H."/>
                <person name="Spagnolo L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="22227115"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2011.12.013"/>
        </citation>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION IN CMR COMPLEX</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). The CMR complex degrades RNA complementary to the crRNA (target RNA) within UA dinucleotides, generating 3'-OH and 5'-phosphate ends. Activity is dependent on the 8 nt long 5' tag in the crRNA, an unpaired 3' flag on the target RNA, and is stimulated by ATP. Some cleavage of the guide crRNA can also be observed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the CMR ribonucleoprotein complex, consisting of crRNA plus Cmr1/Cmr2/Cmr3/Cmr4/Cmr5/Cmr6 at 1:1 and possibly 3 Cmr7 dimers. A Cmr2/Cmr3/Cmr7 subcomplex without crRNA can also be isolated. It does not cleave target RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR system Cmr3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90365">
        <property type="entry name" value="G90365"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WW9"/>
    <dbReference type="SMR" id="Q97WW9"/>
    <dbReference type="STRING" id="273057.SSO1992"/>
    <dbReference type="PaxDb" id="273057-SSO1992"/>
    <dbReference type="EnsemblBacteria" id="AAK42182">
        <property type="protein sequence ID" value="AAK42182"/>
        <property type="gene ID" value="SSO1992"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1992"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2068"/>
    <dbReference type="eggNOG" id="arCOG02665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_884601_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WW9"/>
    <dbReference type="PhylomeDB" id="Q97WW9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09656">
        <property type="entry name" value="Cmr3_III-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019117">
        <property type="entry name" value="CRISPR-assoc_protein_Cmr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010165">
        <property type="entry name" value="CRISPR-Cmr3_IIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052875">
        <property type="entry name" value="CRISPR_assoc_ribonuclease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01888">
        <property type="entry name" value="cas_cmr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37169:SF2">
        <property type="entry name" value="CRISPR SYSTEM CMR SUBUNIT CMR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37169">
        <property type="entry name" value="CRISPR SYSTEM ENDORIBONUCLEASE CSX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09700">
        <property type="entry name" value="Cas_Cmr3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418076" description="CRISPR system CMR subunit Cmr3">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="22227115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="314" mass="36132" checksum="BC1A8814E3C3992E" modified="2001-10-01" version="1">MMKRVLIKPLEPLMFRSQGEFEPLITGSHTAAQSLIIPRPSTIAGMLGYILFNKSSGTGDWLSDLTNLLDTIYGTFIETNGEYLFPLRMGNHLALVDQQHLINLPILLEKEYEQREKGIYELFYDKNKLFQIINHQDRIGISIDKSTRTVKEHYLYSARYLAFKKEVNYVIFIDNDAISDKINGKIVNFGGENRIAKLEVDDYKVDTSIEEEYYLTLSPILIPDEALDDLLDNISDYVTMGKVDKISLGFDIANTKRKEMLTAILEGSIVKRSIIDFIKKEINNNLLDRFTKYEKIGYNTLMNLCKLAFRKILS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WX2</accession>
    <name>CMR1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR system CMR subunit Cmr1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Type III-B/RAMP module RAMP protein Cmr1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cmr1</name>
        <name type="ordered locus">SSO1989</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="Mol. Cell" volume="45" first="303" last="313">
            <title>Structure and mechanism of the CMR complex for CRISPR-mediated antiviral immunity.</title>
            <authorList>
                <person name="Zhang J."/>
                <person name="Rouillon C."/>
                <person name="Kerou M."/>
                <person name="Reeks J."/>
                <person name="Brugger K."/>
                <person name="Graham S."/>
                <person name="Reimann J."/>
                <person name="Cannone G."/>
                <person name="Liu H."/>
                <person name="Albers S.V."/>
                <person name="Naismith J.H."/>
                <person name="Spagnolo L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="22227115"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2011.12.013"/>
        </citation>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION IN CMR COMPLEX</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). The CMR complex degrades RNA complementary to the crRNA (target RNA) within UA dinucleotides, generating 3'-OH and 5'-phosphate ends. Activity is dependent on the 8 nt long 5' tag in the crRNA, an unpaired 3' flag on the target RNA, and is stimulated by ATP. Some cleavage of the guide crRNA can also be observed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the CMR ribonucleoprotein complex, consisting of crRNA plus Cmr1/Cmr2/Cmr3/Cmr4/Cmr5/Cmr6 at 1:1 and possibly 3 Cmr7 dimers. A Cmr2/Cmr3/Cmr7 subcomplex without crRNA can also be isolated. It does not cleave target RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR system Cmr1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992994.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WX2"/>
    <dbReference type="SMR" id="Q97WX2"/>
    <dbReference type="STRING" id="273057.SSO1989"/>
    <dbReference type="PaxDb" id="273057-SSO1989"/>
    <dbReference type="EnsemblBacteria" id="AAK42179">
        <property type="protein sequence ID" value="AAK42179"/>
        <property type="gene ID" value="SSO1989"/>
    </dbReference>
    <dbReference type="GeneID" id="1453506"/>
    <dbReference type="GeneID" id="27428315"/>
    <dbReference type="KEGG" id="sso:SSO1989"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2065"/>
    <dbReference type="eggNOG" id="arCOG03893">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_584790_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WX2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09657">
        <property type="entry name" value="Cmr1_III-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007522">
        <property type="entry name" value="CRISPR-assoc_prot_TM1795"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01894">
        <property type="entry name" value="cas_TM1795_cmr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418072" description="CRISPR system CMR subunit Cmr1">
        <location>
            <begin position="1"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="22227115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="467" mass="54844" checksum="A17CF7D8D11E4B35" modified="2001-10-01" version="1">MEELLMSFKLKAIYPLTGGYNRHSINEFYEENVRPTEIKGLWRWWNRVLFNTVSYVKEGKLYTYDSIDRLFEDVFGSENKKSAVRLEVITDEGSDNHFELSNVELDNVIDCLKANREEKVNLDFRDNTLIIEIEGSTKIPISFKSNLDIDKIKDLVYKNKLLSFELLGFKSIKIDTKISDKEVIKEILRDLITNYLEYFNIKQEVTFTLNIYLDKSLKHKQNFDAKLKFALHSLLVFILLGGIGRKTSRGFGGLSIVNAECHDGLCGEIYGIVNNMESEKEKKDLATVLPNIIFSQTIEQYFSELINNESYKLRSWNNNSDFFVYYFIKDINILRINRIDTNVNRNGIENILNRISNELSASGNCLKDLIMQEMRRRAFALAFLGNRKFRNIHEIYPRILEFLYANYIKREFVNLIGKERRLSNLRFKILEINNTYYIISYLLYSSYLKDPNSSIKDTLYQFARCVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X05</accession>
    <name>Q97X05_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dolichyl-phosphate mannose synthase related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90361">
        <property type="entry name" value="C90361"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991757.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X05"/>
    <dbReference type="FunCoup" id="Q97X05">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1954"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1954"/>
    <dbReference type="EnsemblBacteria" id="AAK42146">
        <property type="protein sequence ID" value="AAK42146"/>
        <property type="gene ID" value="SSO1954"/>
    </dbReference>
    <dbReference type="GeneID" id="7806412"/>
    <dbReference type="KEGG" id="sso:SSO1954"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2028"/>
    <dbReference type="eggNOG" id="arCOG00895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_9_0_2"/>
    <dbReference type="InParanoid" id="Q97X05"/>
    <dbReference type="PhylomeDB" id="Q97X05"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000284">
        <property type="entry name" value="Glycosyl transferase family 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="3"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="242" mass="28108" checksum="8195E5BAFDB29594" modified="2001-10-01" version="1">MISIIIPAFNEERRIGRTLEKISSTLPNAEIVVVFDGHDNTPEVVKKFPVKLLISESRLGKGGAIKRGINESNFQRVLLLDADFPITEEELDKILSTDADLVIPKRKIVGMPLKRKFLHKGFIIITKILFPSLAKFSDFQAGVKLVNREKVLDILDELIINDFLFDVNLIYGFKRRHYKVKEVEINYIHDESDSKISKKLIKVIILMFLSLIKLRVYYSPFRKILYTKTYLKAQDYILRKLR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-08-29" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X13</accession>
    <name>ILVC3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Putative ketol-acid reductoisomerase 3</fullName>
            <ecNumber>1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Acetohydroxy-acid isomeroreductase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Alpha-keto-beta-hydroxylacyl reductoisomerase 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ilvC3</name>
        <name type="synonym">ilvC-3</name>
        <name type="ordered locus">SSO1942</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Ser-107 is present instead of the conserved His which is expected to be an active site residue.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90360">
        <property type="entry name" value="A90360"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992598.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X13"/>
    <dbReference type="SMR" id="Q97X13"/>
    <dbReference type="FunCoup" id="Q97X13">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1942"/>
    <dbReference type="PaxDb" id="273057-SSO1942"/>
    <dbReference type="EnsemblBacteria" id="AAK42136">
        <property type="protein sequence ID" value="AAK42136"/>
        <property type="gene ID" value="SSO1942"/>
    </dbReference>
    <dbReference type="GeneID" id="1453463"/>
    <dbReference type="KEGG" id="sso:SSO1942"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2016"/>
    <dbReference type="eggNOG" id="arCOG04465">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033821_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X13"/>
    <dbReference type="PhylomeDB" id="Q97X13"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371:SF1">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151402" description="Putative ketol-acid reductoisomerase 3">
        <location>
            <begin position="1"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="1">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="2">
        <location>
            <begin position="183"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="332" mass="37071" checksum="E4861E33EAC6AF22" modified="2001-08-29" version="1">MDKTVLDASLEPLKGKTIAVIGYGNQGRVQANIMRENGLNVIIGNVKDRYYDLAIKEGFEVYDIGEAVKKADVAFLLIPDEIMKEIYEKKIAPILEGKKEFVLDFASGYNVAFGLIRPPKNVDTVMVAPRMVGEGIMDLHKQGKGYPVLLGVKQDASGKAWDYAKAIAKGIGAIPGGIAVISSFEEEALLDLMSEHTWVPILFGAIKACFDVAVKEYGVSPEAALLEFYASGELVEIARLIAEEGIFNQMVHHSTTSQYGTLTRMFKYYDLVKEIVKDEAKYIWDGSFAKEWTLEQQAGYPVFYRLWELAVQSEMAKAEKELYKILRRKVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X27</accession>
    <name>Q97X27_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in partial transposon ISC1913</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO9270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99358">
        <property type="entry name" value="B99358"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X27"/>
    <dbReference type="STRING" id="273057.SSO9270"/>
    <dbReference type="PaxDb" id="273057-SSO9270"/>
    <dbReference type="EnsemblBacteria" id="AAK42121">
        <property type="protein sequence ID" value="AAK42121"/>
        <property type="gene ID" value="SSO9270"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO9270"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2001"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_4_1_2"/>
    <dbReference type="InParanoid" id="Q97X27"/>
    <dbReference type="PhylomeDB" id="Q97X27"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="98" mass="11136" checksum="C2CD7B76DCB514DB" modified="2001-10-01" version="1">MFYIRSVDIILITYKDRLTRFGFEYLEEFFSTMGVRIEVVLGEEPKDATQELVEDLISIITSFAGKIYGIRSHKKTVLVQGVKKLIGELSGEDSEVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XA3</accession>
    <name>Q97XA3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1845</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90347">
        <property type="entry name" value="H90347"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XA3"/>
    <dbReference type="PaxDb" id="273057-SSO1845"/>
    <dbReference type="EnsemblBacteria" id="AAK42039">
        <property type="protein sequence ID" value="AAK42039"/>
        <property type="gene ID" value="SSO1845"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1845"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1903"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113185_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XA3"/>
    <dbReference type="PhylomeDB" id="Q97XA3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="201" mass="22900" checksum="69C336CC7654363C" modified="2001-10-01" version="1">MTKESAREEYYKALEKAVNEVILSMTGTRKDVAKRLLLGAVVGRNATEIAQEAEMDYETVLNNLDKAAQAKLIEVVKKLVGDHPVLLIIDDTHDHKLYARVMPVSRNGAQIFYCRAHKRFESAIQLLVIGVKDLVNNQIYVIHIIAYIPRKVEEELKRRGEEVKFRTKIDALLEFLSSLSGLNVKSKVFDSWYVSMGISFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XH5</accession>
    <name>Q97XH5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin (Amino-end) (Zfx-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">zfx-2</name>
        <name evidence="2" type="ordered locus">SSO8869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90337">
        <property type="entry name" value="H90337"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XH5"/>
    <dbReference type="STRING" id="273057.SSO8869"/>
    <dbReference type="PaxDb" id="273057-SSO8869"/>
    <dbReference type="EnsemblBacteria" id="AAK41959">
        <property type="protein sequence ID" value="AAK41959"/>
        <property type="gene ID" value="SSO8869"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8869"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1813"/>
    <dbReference type="eggNOG" id="arCOG04548">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3094304_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XH5"/>
    <dbReference type="PhylomeDB" id="Q97XH5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="51" mass="5492" checksum="DABE505B0EFF934D" modified="2001-10-01" version="1">MGIDPNYRQSRQVVGEHEGHKIYGPVDEPKVLGVHGTIVGVDFDVCIADGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XJ8</accession>
    <name>Q97XJ8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1736</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90334">
        <property type="entry name" value="G90334"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XJ8"/>
    <dbReference type="PaxDb" id="273057-SSO1736"/>
    <dbReference type="EnsemblBacteria" id="AAK41934">
        <property type="protein sequence ID" value="AAK41934"/>
        <property type="gene ID" value="SSO1736"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1736"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1780"/>
    <dbReference type="eggNOG" id="arCOG11994">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172293_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XJ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="111" mass="12526" checksum="A03E43B0880BDB26" modified="2001-10-01" version="1">MTFYNYSRWGSKGAENFASLRRRWIAPFVEKFKNSDIDTLLMTVTDGVSVTEAPRLKRGSMRYASAPDNDGTDVKYHAGWEPPLVPVELHPLPVLWQGGAVKQEAPSFRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-01-24" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XM5</accession>
    <name>Q97XM5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial transposase ISC1359</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1688</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90330">
        <property type="entry name" value="C90330"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XM5"/>
    <dbReference type="PaxDb" id="273057-SSO1688"/>
    <dbReference type="EnsemblBacteria" id="AAK41898">
        <property type="protein sequence ID" value="AAK41898"/>
        <property type="gene ID" value="SSO1688"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1688"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1739"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067987_1_0_2"/>
    <dbReference type="InParanoid" id="Q97XM5"/>
    <dbReference type="PhylomeDB" id="Q97XM5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="89"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="211" mass="24076" checksum="97229CE747E44B4B" modified="2001-10-01" version="1">MKHENTTKPAKFNRDFARSALKIIYSVLTKILFPEELLSALLKASGSYLSRLGKDGRRALRKLNAVQVEDVRDALRKMGRMTLRGVRDRRVAVDFHAIPQYHANKSFLSRIKPTKGTSWGLVQAAIFLLGRTRSFLDVIPVTVKNIAEGFKAVMEVIVKELEEDKLRLVMVFADREFAVNEVIRYLLELGLDFVISAKAQMYKKYKGMLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-01-24" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XN3</accession>
    <name>Q97XN3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial transposase in ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90329">
        <property type="entry name" value="C90329"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XN3"/>
    <dbReference type="STRING" id="273057.SSO1681"/>
    <dbReference type="PaxDb" id="273057-SSO1681"/>
    <dbReference type="EnsemblBacteria" id="AAK41890">
        <property type="protein sequence ID" value="AAK41890"/>
        <property type="gene ID" value="SSO1681"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1681"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1731"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2165379_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XN3"/>
    <dbReference type="PhylomeDB" id="Q97XN3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="1">
        <location>
            <begin position="37"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="110" mass="13087" checksum="1FFA668FCA1D34D2" modified="2001-10-01" version="1">MIVHRDFPLDKVKRIIVKLEPSGRIYIIFVIDYEFKALPFTGKVVAIDVGIEKLVTTSDGQYFPNLKPFERALTKVRELHRSLSRKRFLSHNWFKAKVKLARAYEHYYFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XR0</accession>
    <name>Q97XR0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">First ORF in transposon ISC1250</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1650</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90325">
        <property type="entry name" value="H90325"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XR0"/>
    <dbReference type="PaxDb" id="273057-SSO1650"/>
    <dbReference type="EnsemblBacteria" id="AAK41863">
        <property type="protein sequence ID" value="AAK41863"/>
        <property type="gene ID" value="SSO1650"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1650"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1698"/>
    <dbReference type="eggNOG" id="arCOG04918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129669_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XR0"/>
    <dbReference type="PhylomeDB" id="Q97XR0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217:SF7">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="137" mass="16032" checksum="6785634AA8B17AF4" modified="2001-10-01" version="1">METITIDEKTLYNLTQPSEVIEELTHLNGNLTLVPNILSEEASLNKSVKEIEKIALEDTDKTSPKHVRGIRRKDYTKYRFLKGFKIMLKGREVIYKLEWISVELPVLYNKGRVKTQVEEMLLREEKVFKALMTIYSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XV8</accession>
    <name>Q97XV8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ORF in partial transposon ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8458</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90319">
        <property type="entry name" value="D90319"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XV8"/>
    <dbReference type="STRING" id="273057.SSO8458"/>
    <dbReference type="PaxDb" id="273057-SSO8458"/>
    <dbReference type="EnsemblBacteria" id="AAK41811">
        <property type="protein sequence ID" value="AAK41811"/>
        <property type="gene ID" value="SSO8458"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8458"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1639"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2930380_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XV8"/>
    <dbReference type="PhylomeDB" id="Q97XV8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="60" mass="6882" checksum="86D66A1AAFD27DFF" modified="2001-10-01" version="1">MPVGEFPKGKYLVEYQGIPIKLLVVEDYKGLGKRYFFSTNLNDTERILSLVGRIDGISRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y63</accession>
    <name>Q97Y63_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease VapC</fullName>
            <shortName evidence="5">RNase VapC</shortName>
            <ecNumber evidence="5">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Putative toxin VapC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">vapC</name>
        <name evidence="7" type="ordered locus">SSO1483</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Toxic component of a toxin-antitoxin (TA) system. An RNase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90306">
        <property type="entry name" value="E90306"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y63"/>
    <dbReference type="STRING" id="273057.SSO1483"/>
    <dbReference type="PaxDb" id="273057-SSO1483"/>
    <dbReference type="EnsemblBacteria" id="AAK41708">
        <property type="protein sequence ID" value="AAK41708"/>
        <property type="gene ID" value="SSO1483"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1483"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1522"/>
    <dbReference type="eggNOG" id="arCOG02730">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153004_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y63"/>
    <dbReference type="PhylomeDB" id="Q97Y63"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18684">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00265">
        <property type="entry name" value="VapC_Nob1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022907">
        <property type="entry name" value="VapC_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39664">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39664:SF2">
        <property type="entry name" value="NUCLEIC ACID-BINDING PROTEIN, CONTAINING PIN DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0800">Toxin</keyword>
    <keyword evidence="1 5" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="domain" description="PIN" evidence="6">
        <location>
            <begin position="19"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="148" mass="16910" checksum="48C0303F051089C7" modified="2001-10-01" version="1">MEEKLQWRRLRKSQVRPLKMIVVDTNVLIYATLEDSEFHTQSLEIIEGSDIIVPQIVVFEYIKVLSEIVQNLDFIKTKISELNNFVVVCEDLNTIALALRLLAELKLSLKDINDMIILTAAIKTNSSIATFDQKLRKIADKKGVKVLP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y96</accession>
    <name>CAS6A_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated endoribonuclease Cas6 1</fullName>
            <ecNumber>3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cas6a</name>
        <name type="ordered locus">SSO1437</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="J. Biol. Chem." volume="286" first="21643" last="21656">
            <title>Structural and functional characterization of an archaeal clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for antiviral defense (CASCADE).</title>
            <authorList>
                <person name="Lintner N.G."/>
                <person name="Kerou M."/>
                <person name="Brumfield S.K."/>
                <person name="Graham S."/>
                <person name="Liu H."/>
                <person name="Naismith J.H."/>
                <person name="Sdano M."/>
                <person name="Peng N."/>
                <person name="She Q."/>
                <person name="Copie V."/>
                <person name="Young M.J."/>
                <person name="White M.F."/>
                <person name="Lawrence C.M."/>
            </authorList>
            <dbReference type="PubMed" id="21507944"/>
            <dbReference type="DOI" id="10.1074/jbc.M111.238485"/>
        </citation>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35091 / DSM 1616 / JCM 8930 / NBRC 15331 / P1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the aCascade ribonucleoprotein complex, minimally composed of Csa2 and Cas5a, which binds crRNA. Other possible components of aCascade in strain P1 are Cas6b (SSO1437) and Csa5 (SSO1443), while SSO1399, Cas5b (SSO1400) and SSO1401 have sometimes been seen weakly associated. Csa2 is probably the major RNA-binding subunit. The Csa2-Cas5a-crRNA complex also binds target DNA homologous to crRNA, probably forming an R-loop. Purified aCascade forms a filament about 6 nm in width.</text>
    </comment>
    <comment type="miscellaneous">
        <text>The aCascade complex was purified from strain P1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR-associated endoribonuclease Cas6 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90301">
        <property type="entry name" value="G90301"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923402.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3ZFV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B/C/D=2-287"/>
    </dbReference>
    <dbReference type="PDBsum" id="3ZFV"/>
    <dbReference type="AlphaFoldDB" id="Q97Y96"/>
    <dbReference type="SMR" id="Q97Y96"/>
    <dbReference type="STRING" id="273057.SSO1437"/>
    <dbReference type="PaxDb" id="273057-SSO1437"/>
    <dbReference type="EnsemblBacteria" id="AAK41670">
        <property type="protein sequence ID" value="AAK41670"/>
        <property type="gene ID" value="SSO1437"/>
    </dbReference>
    <dbReference type="GeneID" id="1454449"/>
    <dbReference type="KEGG" id="sso:SSO1437"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1466"/>
    <dbReference type="eggNOG" id="arCOG01439">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_929391_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y96"/>
    <dbReference type="EvolutionaryTrace" id="Q97Y96"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09652">
        <property type="entry name" value="Cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041165">
        <property type="entry name" value="Cas6_N_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019267">
        <property type="entry name" value="CRISPR-assoc_Cas6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010156">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01877">
        <property type="entry name" value="cas_cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17952">
        <property type="entry name" value="Cas6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10040">
        <property type="entry name" value="CRISPR_Cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000417883" description="CRISPR-associated endoribonuclease Cas6 1">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="3"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="25"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="35"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="68"/>
            <end position="71"/>
        </location>
    </feature>
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        <location>
            <begin position="81"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="100"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="107"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="131"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="148"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="154"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="157"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="173"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="194"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="209"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="247"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="269"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="276"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="21507944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3ZFV"/>
        </source>
    </evidence>
    <sequence length="287" mass="32425" checksum="13E4FE6603783A94" modified="2001-10-01" version="1">MPLIFKIGYNVIPLQDVILPTPSSKVLKYLIQSGKLLPSLNNLITSRDKYKPIFISHLGLNQRRIFQTNGNLKTISRGSKLSSTIAFSTQVNVLPELDEGVFETIYGKFHITIESVEIVEVEKLKEEVEKHMNDNIRVRFISPTLLSSKVLLPPSLSERYKRVNAGYSTLPSVGLIVAYAYNVYCNLIGKKEVEVRAFKFGVISNALSRIIGYDLHPVTIVIGEDSKGNLRKARGVMGWIEFDIPDEKLKRRALRYLLASSYLGIGRSRGIGFGEIKLEFIKREENH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YA5</accession>
    <name>Q97YA5_SACS2</name>
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        <recommendedName>
            <fullName evidence="2">CRISPR type III-associated protein domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="ordered locus">SSO1427</name>
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    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2023" name="Curr. Res. Struct. Biol." volume="5" first="100098" last="100098">
            <title>Structure of the &lt;i&gt;Saccharolobus solfataricus&lt;/i&gt; type III-D CRISPR effector.</title>
            <authorList>
                <person name="Cannone G."/>
                <person name="Kompaniiets D."/>
                <person name="Graham S."/>
                <person name="White M.F."/>
                <person name="Spagnolo L."/>
            </authorList>
            <dbReference type="PubMed" id="36843655"/>
            <dbReference type="DOI" id="10.1016/j.crstbi.2023.100098"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (3.50 ANGSTROMS) OF 3-274</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="F90300"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923393.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="8BMW">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="J=3-274"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YA5"/>
    <dbReference type="SMR" id="Q97YA5"/>
    <dbReference type="STRING" id="273057.SSO1427"/>
    <dbReference type="PaxDb" id="273057-SSO1427"/>
    <dbReference type="EnsemblBacteria" id="AAK41661">
        <property type="protein sequence ID" value="AAK41661"/>
        <property type="gene ID" value="SSO1427"/>
    </dbReference>
    <dbReference type="GeneID" id="1454440"/>
    <dbReference type="KEGG" id="sso:SSO1427"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1453"/>
    <dbReference type="eggNOG" id="arCOG02660">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1044350_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
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    </dbReference>
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    <feature type="domain" description="CRISPR type III-associated protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="223"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="8BMW"/>
        </source>
    </evidence>
    <sequence length="275" mass="30939" checksum="09F548E7072A7B79" modified="2001-10-01" version="1">MQRSPIRRSNYTPRNEKGLEGVIELQLNVVSDYLHVGSGKYDVEVMRSVSDVKRLVEDYLSGGNKRIPNNVDQYFSMVAFLMVRNKDNVVIPGSTIKGMVRSRLELSVPGSCYIVTGHSTSSSAVYKRIFNPDPNRGSDRFDVNKFPQVCPVCDLLGNMGLASRVSLSDFVMTSGKVDYVNVKGRDYEVVTKGSIFAGKVLYKSLKPVEIGMLLYGFGFVKDCNGSKVMLLGRFKFSDKRFGRVKFSLKTPIADCNKLVSDFVKQFNPRYINEEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YI9</accession>
    <name>Q97YI9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1332</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90289">
        <property type="entry name" value="C90289"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YI9"/>
    <dbReference type="PaxDb" id="273057-SSO1332"/>
    <dbReference type="EnsemblBacteria" id="AAK41570">
        <property type="protein sequence ID" value="AAK41570"/>
        <property type="gene ID" value="SSO1332"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1332"/>
    <dbReference type="HOGENOM" id="CLU_2313924_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YI9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="99" mass="11372" checksum="6F705BD5B8ABCE6D" modified="2001-10-01" version="1">MGSIDSTKFPSSSKNSIRHAYFQSDITLSMSSHFSSAILIYMGPKHEYTFAIINLITALRFKKHFYSILYVKSEDSRGILEFGMFSSSRLIRYLIEDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YL7</accession>
    <name>Q97YL7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH bat-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90285">
        <property type="entry name" value="G90285"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923299.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YL7"/>
    <dbReference type="PaxDb" id="273057-SSO1306"/>
    <dbReference type="EnsemblBacteria" id="AAK41542">
        <property type="protein sequence ID" value="AAK41542"/>
        <property type="gene ID" value="SSO1306"/>
    </dbReference>
    <dbReference type="GeneID" id="1454321"/>
    <dbReference type="KEGG" id="sso:SSO1306"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1306"/>
    <dbReference type="eggNOG" id="arCOG02272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104910_1_0_2"/>
    <dbReference type="InParanoid" id="Q97YL7"/>
    <dbReference type="PhylomeDB" id="Q97YL7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH bat-type" evidence="1">
        <location>
            <begin position="165"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="234" mass="28052" checksum="AEBFCE29B3E54C23" modified="2001-10-01" version="1">MSLFNLSLVTFEIDHEDCWSKLTSSFPVQVKTIFAKPSKGKDYILGMDEVKTYNRRVFKDFLKSFKKEKSIYEIIQILELDSRRGIYRILFKERYENMIMSIIENYMTLYIKDLIKEGNERLLLIMPSSDVTMLKRDLESIGKIHYFNAKLVNFNDFVPTFFDFSEQERNAVLHAIRLGYYEYPRRINLEELGKIMGISKPTLEEYLRKAEKKIMSKIFREFYQQDLLFHSPDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YQ9</accession>
    <name>Q97YQ9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1262</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90280">
        <property type="entry name" value="E90280"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990018.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YQ9"/>
    <dbReference type="STRING" id="273057.SSO1262"/>
    <dbReference type="PaxDb" id="273057-SSO1262"/>
    <dbReference type="EnsemblBacteria" id="AAK41500">
        <property type="protein sequence ID" value="AAK41500"/>
        <property type="gene ID" value="SSO1262"/>
    </dbReference>
    <dbReference type="GeneID" id="1454279"/>
    <dbReference type="KEGG" id="sso:SSO1262"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1258"/>
    <dbReference type="eggNOG" id="arCOG08324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1559380_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YQ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="178" mass="20417" checksum="7FDB4796AD857726" modified="2001-10-01" version="1">MRFGLLTTIGFSLLVLSVLSINSPIQSLISISNPYSIAVPKIAYVTARLFENDSNVTIYVQIIHNGYTKIVKLPSYFKLTPGKWEFSIYNETFPITLTKVVNATVVNKSNDIVYVYEYQKIEKYQEIVTTKNTTYPIDLEITIHKVDILQYKEIAEVLGVILIVIDIITLIVIRFKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YU7</accession>
    <name>Q97YU7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">pyruvate synthase</fullName>
            <ecNumber evidence="1">1.2.7.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">porG-1</name>
        <name evidence="5" type="ordered locus">SSO1208</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2 oxidized [2Fe-2S]-[ferredoxin] + pyruvate + CoA = 2 reduced [2Fe-2S]-[ferredoxin] + acetyl-CoA + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12765"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="1.2.7.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.2.7.1" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99275">
        <property type="entry name" value="A99275"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YU7"/>
    <dbReference type="FunCoup" id="Q97YU7">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1208"/>
    <dbReference type="PaxDb" id="273057-SSO1208"/>
    <dbReference type="EnsemblBacteria" id="AAK41456">
        <property type="protein sequence ID" value="AAK41456"/>
        <property type="gene ID" value="SSO1208"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1208"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1207"/>
    <dbReference type="eggNOG" id="arCOG01603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087284_2_0_2"/>
    <dbReference type="InParanoid" id="Q97YU7"/>
    <dbReference type="PhylomeDB" id="Q97YU7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051626">
        <property type="entry name" value="Oxidoreductase_gamma_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43366">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43366:SF1">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="4">
        <location>
            <begin position="20"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00049357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="190" mass="20965" checksum="4C33DC1841B18AE7" modified="2001-10-01" version="1">MEYYNCDKMTLIELALRGRGGQGIVTAGELMTKAMVLEDKYAQSIPFFGGERRGAPVVSFVRLSDKPILLHREVYNPDGVAIFDVSMIQLINVTEGLKENGFLLLNTNTPKRIWKNEYVVDATNIAKELGLIVAGWAIVNTAMIGALARILGQPSLHSLEEAVKEEFPGKIGELNAEAVEIGYKEVKRVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YV3</accession>
    <name>Q97YV3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1048</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO7399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90274">
        <property type="entry name" value="B90274"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YV3"/>
    <dbReference type="EnsemblBacteria" id="AAK41449">
        <property type="protein sequence ID" value="AAK41449"/>
        <property type="gene ID" value="SSO7399"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7399"/>
    <dbReference type="eggNOG" id="arCOG02129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3094303_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YV3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="51" mass="6046" checksum="108A4A6B98CF8720" modified="2001-10-01" version="1">MDNENTQDSTTRTRNRIQEIQTIRTSPRTRIQPSKTQTYQHPSRKGQVGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YX9</accession>
    <name>Q97YX9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid permease related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1173</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90270">
        <property type="entry name" value="F90270"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991567.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YX9"/>
    <dbReference type="STRING" id="273057.SSO1173"/>
    <dbReference type="TCDB" id="2.A.3.15.3">
        <property type="family name" value="the amino acid-polyamine-organocation (apc) family"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1173"/>
    <dbReference type="EnsemblBacteria" id="AAK41421">
        <property type="protein sequence ID" value="AAK41421"/>
        <property type="gene ID" value="SSO1173"/>
    </dbReference>
    <dbReference type="GeneID" id="1454200"/>
    <dbReference type="KEGG" id="sso:SSO1173"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1166"/>
    <dbReference type="eggNOG" id="arCOG03654">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031607_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YX9"/>
    <dbReference type="PhylomeDB" id="Q97YX9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="386"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="417" mass="45685" checksum="DAEE35DC3EB58FC3" modified="2001-10-01" version="1">MSRRKLSVFEAFSLSFGGQAPFTSIITFGTIGLQLGGSFLTIATIIGTILVLVNGIVIYRLSLRYSQHGGYFTYAFYSLTERLGLVTGWLFLLYAFSYGGTLLAGSIYIITSYLKISADLVAFLVILFSAFLVIRGLDVSVKYAEFISIAEIVAIIVSSVVLLLGTKPSFNLTIPTNPFLVILYAIGMPIGYGNLNPMSEDIKNAKKIVGIITVIVILLGGLLSALLFYASALYGTDLIEILLDKVGFIFPYLIFSALNGGILGGIAYIIAMSRILHAMSLKNLMPSIISSVKYNRPYNAEVISLIIYTVILFLLTHFVGLYTTFLVLGGLTVLSYLIISLSANLSLFRIALKKLRKRKMEITIAITSTLLSLIILVYSIQENTPIINYIFFAWIIAGFIYAEVLEIAGNNGKDDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YY2</accession>
    <name>Q97YY2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sugar transport protein, hypothetical</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO1170</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90270">
        <property type="entry name" value="C90270"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YY2"/>
    <dbReference type="FunCoup" id="Q97YY2">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1170"/>
    <dbReference type="PaxDb" id="273057-SSO1170"/>
    <dbReference type="EnsemblBacteria" id="AAK41418">
        <property type="protein sequence ID" value="AAK41418"/>
        <property type="gene ID" value="SSO1170"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1170"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1163"/>
    <dbReference type="eggNOG" id="arCOG00157">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_3_2"/>
    <dbReference type="InParanoid" id="Q97YY2"/>
    <dbReference type="PhylomeDB" id="Q97YY2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.370">
        <property type="entry name" value="MalF N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035277">
        <property type="entry name" value="MalF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050809">
        <property type="entry name" value="UgpAE/MalFG_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43227:SF7">
        <property type="entry name" value="ARABINOOLIGOSACCHARIDES TRANSPORT SYSTEM PERMEASE PROTEIN ARAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43227">
        <property type="entry name" value="BLL4140 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160964">
        <property type="entry name" value="MalF N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="72"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="297" mass="34170" checksum="7A4360684C1E0B72" modified="2001-10-01" version="1">MVNQEMKKEKLFSLFYILPIVIVTVFLFVFPLLYSVYISFTNLSLLHFLTYSYVGLRNYLIIFQYGYFLELLKNTLIWTIGSLLTMMSLGFVLALILNQTDLKGRSIFYAILILPWAFPGFISILIWQGLWVDPYGMMNRLILPLLHLPKVNSLTSTTDAWIELIATNDWLSFPYFMTVFYSALQSIPRELYDIADLDGANAFTKFLTITLPSLKRTIAFVFITSFVFTWNNFYPIYVLTGGGPGISTETFIVYAYQEAFSYNNFALAAAWSIISTIFVIVLGIIVIKYTRILDSFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-11-27" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YY5</accession>
    <name>Q97YY5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GH15-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90269">
        <property type="entry name" value="H90269"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YY5"/>
    <dbReference type="STRING" id="273057.SSO1167"/>
    <dbReference type="CAZy" id="GH15">
        <property type="family name" value="Glycoside Hydrolase Family 15"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1167"/>
    <dbReference type="EnsemblBacteria" id="AAK41415">
        <property type="protein sequence ID" value="AAK41415"/>
        <property type="gene ID" value="SSO1167"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1167"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1159"/>
    <dbReference type="eggNOG" id="arCOG03286">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_854238_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YY5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH15-like" evidence="1">
        <location>
            <begin position="235"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="325" mass="36459" checksum="826DFF3B2A718DA7" modified="2001-10-01" version="1">MRGEEIIFIILIFLSFLNPLLTFSATSSLKYYSPSYLLLNNWKNQSIWIVTGIPVPNPQLNGFPLFNTSIRNFYIGKYGIVNLTVSFNVITTLAYLNLNNTINLASTSGNISIIMPPKTPYILILLNANSTTSIDIATNTSISINNSIISVDTTPQIYILTNTSYLIQNKTVKINVNKGEWFLELSIGEKPNFNISGLLELNDIEVEKWLNASKLPNSLPNDLLKEYYLSLLLIKDDQNPYLGTFAASPSPIYLFSWVRDSAFSAMALQLAGHYDSALKFWLWMSHAEQLQPGVWYTRYDFYNGNPDTTFGIPGAAQESIANCIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YZ2</accession>
    <name>Q97YZ2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90269">
        <property type="entry name" value="A90269"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991587.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YZ2"/>
    <dbReference type="STRING" id="273057.SSO1157"/>
    <dbReference type="PaxDb" id="273057-SSO1157"/>
    <dbReference type="EnsemblBacteria" id="AAK41408">
        <property type="protein sequence ID" value="AAK41408"/>
        <property type="gene ID" value="SSO1157"/>
    </dbReference>
    <dbReference type="GeneID" id="1454187"/>
    <dbReference type="KEGG" id="sso:SSO1157"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1151"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_2_2_2"/>
    <dbReference type="InParanoid" id="Q97YZ2"/>
    <dbReference type="PhylomeDB" id="Q97YZ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07721">
        <property type="entry name" value="yflN-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF18">
        <property type="entry name" value="METALLO-HYDROLASE MJ0296-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="213" mass="23712" checksum="F422BE672A9EFF90" modified="2001-10-01" version="1">MKVFEIPLSFVKVFLIETNENGLILIDSGTPGSGRRIIEGITRLGKNPNKIKSVIFTHSHVDHIGGAYEVKRFVNDAKFGIDNNGVNYLKNGKIREPVLHSSALKVIFSIGRPFFFRKFNGVNVDFVLEEGELVKGVEIIKTPGHTNDSISIYLPELNAIIVGDTLQGDKRGLKYPNIYEDFDGLRKSVENIKSFNPSMVYVSHGVSSSKFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z20</accession>
    <name>Q97Z20_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1121</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90265">
        <property type="entry name" value="B90265"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989753.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z20"/>
    <dbReference type="STRING" id="273057.SSO1121"/>
    <dbReference type="PaxDb" id="273057-SSO1121"/>
    <dbReference type="EnsemblBacteria" id="AAK41377">
        <property type="protein sequence ID" value="AAK41377"/>
        <property type="gene ID" value="SSO1121"/>
    </dbReference>
    <dbReference type="GeneID" id="1454156"/>
    <dbReference type="KEGG" id="sso:SSO1121"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1117"/>
    <dbReference type="eggNOG" id="arCOG05917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2056095_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z20"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="122" mass="14149" checksum="9CFCBAA01411610C" modified="2001-10-01" version="1">MWINIRVKMGKLEDYLKKKGFSLVNEGKRERVVMDDYEFFIENLTILLPIPLPTGKESLDDLIGMGTRYARASRISQGLGAPLEYELNGTTIYIIKRFQNREDLENSIIKSLEGIESLRYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z55</accession>
    <name>Q97Z55_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S-layer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90260">
        <property type="entry name" value="F90260"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989715.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z55"/>
    <dbReference type="STRING" id="273057.SSO1079"/>
    <dbReference type="PaxDb" id="273057-SSO1079"/>
    <dbReference type="EnsemblBacteria" id="AAK41341">
        <property type="protein sequence ID" value="AAK41341"/>
        <property type="gene ID" value="SSO1079"/>
    </dbReference>
    <dbReference type="GeneID" id="1454120"/>
    <dbReference type="KEGG" id="sso:SSO1079"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1079"/>
    <dbReference type="eggNOG" id="arCOG02089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_340005_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z55"/>
    <dbReference type="PhylomeDB" id="Q97Z55"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902:SF3">
        <property type="entry name" value="NPCBM-ASSOCIATED, NEW3 DOMAIN OF ALPHA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902">
        <property type="entry name" value="S-LAYER DOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="807"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="835" mass="92358" checksum="BD28B5418BAF7250" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZA3</accession>
    <name>Q97ZA3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90254">
        <property type="entry name" value="C90254"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZA3"/>
    <dbReference type="STRING" id="273057.SSO1027"/>
    <dbReference type="PaxDb" id="273057-SSO1027"/>
    <dbReference type="EnsemblBacteria" id="AAK41290">
        <property type="protein sequence ID" value="AAK41290"/>
        <property type="gene ID" value="SSO1027"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1027"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1019"/>
    <dbReference type="eggNOG" id="arCOG07213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1902027_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZA3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="136" mass="15102" checksum="F2D68438DC60AA0B" modified="2001-10-01" version="1">MIIMIGKVIVVLAIILVGVFLLTHTNLFYHPQTPVSKGQEYYTTTNVQNITYQFGIISNVSTNYTVPLKLSYGNVTIKVMGHNAFNLTVVNNSTVIYSGIVDRYFSKTFIIGGDVKLVFSSRQGLEVNVTIFDVLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZQ1</accession>
    <name>RL37_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein eL37</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>50S ribosomal protein L37e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rpl37e</name>
        <name type="ordered locus">SSO6453</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL37 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90224">
        <property type="entry name" value="E90224"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991337.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZQ1"/>
    <dbReference type="SMR" id="Q97ZQ1"/>
    <dbReference type="FunCoup" id="Q97ZQ1">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO6453"/>
    <dbReference type="PaxDb" id="273057-SSO6453"/>
    <dbReference type="EnsemblBacteria" id="AAK41052">
        <property type="protein sequence ID" value="AAK41052"/>
        <property type="gene ID" value="SSO6453"/>
    </dbReference>
    <dbReference type="GeneID" id="42364250"/>
    <dbReference type="KEGG" id="sso:SSO6453"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.754"/>
    <dbReference type="eggNOG" id="arCOG04126">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208825_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZQ1"/>
    <dbReference type="PhylomeDB" id="Q97ZQ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000003">
        <property type="entry name" value="50S ribosomal protein L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00547">
        <property type="entry name" value="Ribosomal_eL37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001569">
        <property type="entry name" value="Ribosomal_eL37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018267">
        <property type="entry name" value="Ribosomal_eL37_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003214">
        <property type="entry name" value="PRK04179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768:SF0">
        <property type="entry name" value="RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01907">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01077">
        <property type="entry name" value="RIBOSOMAL_L37E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000139739" description="Large ribosomal subunit protein eL37">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="2">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="61" mass="7145" checksum="FF9BFCDE4FD4805A" modified="2001-10-01" version="1">MKGTPSFGKMNKSHTHIRCRRCGRNAYNVSKHYCAACGFGRTKKIRRYSWQNKKVNGVRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZU2</accession>
    <name>Q97ZU2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0485</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90193">
        <property type="entry name" value="G90193"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923000.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZU2"/>
    <dbReference type="STRING" id="273057.SSO0485"/>
    <dbReference type="PaxDb" id="273057-SSO0485"/>
    <dbReference type="EnsemblBacteria" id="AAK40806">
        <property type="protein sequence ID" value="AAK40806"/>
        <property type="gene ID" value="SSO0485"/>
    </dbReference>
    <dbReference type="GeneID" id="1455616"/>
    <dbReference type="KEGG" id="sso:SSO0485"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.479"/>
    <dbReference type="eggNOG" id="arCOG04233">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056492_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZU2"/>
    <dbReference type="PhylomeDB" id="Q97ZU2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01143">
        <property type="entry name" value="YvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050902">
        <property type="entry name" value="ABC_Transporter_SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535:SF34">
        <property type="entry name" value="MOLYBDATE-BINDING PROTEIN MOLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="2">
        <location>
            <begin position="42"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="318" mass="34839" checksum="C79B6583F8D799BA" modified="2001-10-01" version="1">MPSVKAIGTIVAIIIVVSVISVYYYYKSSSTLQPATVNNNLRIVSLAPSDTQILISLGLGKYIVGTDYYSYQLLQSLNLTKDIPSNITVFSQISPPNISGLLLLHPTVIVVEKGLIGSYIEQMKEAGLNVLITNNDFASTYSQIENCILKVGEYFNVTSKAQQLIDWMNQKISDFSTAGNVSVAYLLWICPDLSFYTAGGNVFINSIIVQAGGINVFANYSGYPLLTPSPLLIIKPSVVIAQEVYNLTYTNYLISQYKGINSSNLYIIGNLATNLLNEPGPLSVYSIQVIKLIIEGKAPHFISDNWVINNLNVTLPVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZU5</accession>
    <name>Q97ZU5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Twin-arginine translocase TatA/TatE family subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">SSO0482</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90193">
        <property type="entry name" value="D90193"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZU5"/>
    <dbReference type="STRING" id="273057.SSO0482"/>
    <dbReference type="PaxDb" id="273057-SSO0482"/>
    <dbReference type="EnsemblBacteria" id="AAK40803">
        <property type="protein sequence ID" value="AAK40803"/>
        <property type="gene ID" value="SSO0482"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0482"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.476"/>
    <dbReference type="eggNOG" id="arCOG02694">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086034_3_1_2"/>
    <dbReference type="InParanoid" id="Q97ZU5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.3310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006312">
        <property type="entry name" value="TatA/E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01411">
        <property type="entry name" value="tatAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982:SF1">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="81"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK40803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="13078" checksum="3B73B14A19CF109F" modified="2001-10-01" version="1">MKTKFLISQYDFSYKDGKMLTNPYDWIIILVVIAVLFFGASKIPELFRSMGRAVGEFKKGRVEAEMELQQMQAQNLQQPLVQQQQPNVQDLEKQIAELQKQLEELKKSKQNQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2015-10-14" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZW4</accession>
    <name>APBC_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO0460</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1490" last="1497">
            <title>Archaeal ApbC/Nbp35 homologs function as iron-sulfur cluster carrier proteins.</title>
            <authorList>
                <person name="Boyd J.M."/>
                <person name="Drevland R.M."/>
                <person name="Downs D.M."/>
                <person name="Graham D.E."/>
            </authorList>
            <dbReference type="PubMed" id="19114487"/>
            <dbReference type="DOI" id="10.1128/JB.01469-08"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">Although the second Cys residue of the CXXC motif is replaced with Asp, the protein has Fe-S carrier activity, suggesting that individual cysteine residues in the CXXC motif are dispensable for Fe-S cluster binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90191">
        <property type="entry name" value="A90191"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922978.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZW4"/>
    <dbReference type="SMR" id="Q97ZW4"/>
    <dbReference type="FunCoup" id="Q97ZW4">
        <property type="interactions" value="133"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0460"/>
    <dbReference type="PaxDb" id="273057-SSO0460"/>
    <dbReference type="EnsemblBacteria" id="AAK40784">
        <property type="protein sequence ID" value="AAK40784"/>
        <property type="gene ID" value="SSO0460"/>
    </dbReference>
    <dbReference type="GeneID" id="15298046"/>
    <dbReference type="KEGG" id="sso:SSO0460"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.454"/>
    <dbReference type="eggNOG" id="arCOG00585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_1_2"/>
    <dbReference type="InParanoid" id="Q97ZW4"/>
    <dbReference type="PhylomeDB" id="Q97ZW4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001119">
        <property type="entry name" value="Iron-sulfur cluster carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF19">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000433954" description="Iron-sulfur cluster carrier protein">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="52"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="19114487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="19114487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40784.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="32048" checksum="20732413716BB566" modified="2001-10-01" version="1">MSSNPFRIQNPQPQPQRQPRDLRKVNQQVQAVDLKVQMKMKNIKYKIGVVSGKGGVGKSFVSSNLAMAIAASGRKVGIVDVDFHGPSVPKMLGVRGQMLTADDKGINPVIGPFGIKVVSIDFLLPRDDTPVVWRGAIKHSAIKQFLGDVNWGELDYLIIDMPPGTGDEALSIAQLVPGITGFVIVTIPSEVSTLAVKKSINFARTVNTKILGVVENMSHFVCPSDGKVYYIFGEGKGKKMAEEMGVDLLGQVPLDPSIAEANDAGEPFFLKHPDSPTSKEFLNIADKVIKIVESNQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-02" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q980A0</accession>
    <name>APGM_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2,3-bisphosphoglycerate-independent phosphoglycerate mutase</fullName>
            <shortName evidence="1">BPG-independent PGAM</shortName>
            <shortName evidence="1">Phosphoglyceromutase</shortName>
            <shortName evidence="1">aPGAM</shortName>
            <ecNumber evidence="1">5.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">apgM</name>
        <name type="ordered locus">SSO0417</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90186">
        <property type="entry name" value="B90186"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988763.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980A0"/>
    <dbReference type="SMR" id="Q980A0"/>
    <dbReference type="FunCoup" id="Q980A0">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0417"/>
    <dbReference type="PaxDb" id="273057-SSO0417"/>
    <dbReference type="DNASU" id="1455555"/>
    <dbReference type="EnsemblBacteria" id="AAK40745">
        <property type="protein sequence ID" value="AAK40745"/>
        <property type="gene ID" value="SSO0417"/>
    </dbReference>
    <dbReference type="GeneID" id="1455555"/>
    <dbReference type="KEGG" id="sso:SSO0417"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.412"/>
    <dbReference type="eggNOG" id="arCOG01696">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034906_2_0_2"/>
    <dbReference type="InParanoid" id="Q980A0"/>
    <dbReference type="PhylomeDB" id="Q980A0"/>
    <dbReference type="BRENDA" id="5.4.2.12">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01402_A">
        <property type="entry name" value="ApgM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023665">
        <property type="entry name" value="ApgAM_prokaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00306">
        <property type="entry name" value="apgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003104">
        <property type="entry name" value="PRK04024.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="COFACTOR-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209:SF0">
        <property type="entry name" value="METALLOENZYME DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006392">
        <property type="entry name" value="IPGAM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000138148" description="2,3-bisphosphoglycerate-independent phosphoglycerate mutase">
        <location>
            <begin position="1"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01402"/>
        </source>
    </evidence>
    <sequence length="414" mass="45180" checksum="05270AB0066F4CEB" modified="2001-10-01" version="1">MKQYKILLIVADGLGDRPVSKLNGLTPLEAANKPAITDLLKNSMIGLMDPISPGIIPGSDTSHLSIFGLDPHVYYRGRGAFEALGAGATLSHGDVAFRGNFATVNNDLVVVDRRAGRKLEEGEQLVKELNEKIKEINDVKIKFYKGTEHRIAVVLSGKGISDKVSDTDPHHEGLKVLESKPLEDSNEAIRTAEIINILTRKVFDVLNSSEINKRRIEQGEKPANIVLLRGAAHYVKLPPFSSYTKLKAAAVSATALIKGICRELGMNVITPSGATGGIDTNYNAKAKAAIELLKENDFVFLHIKATDAASHDGLVEEKVKAIERIDRVIGAIIDNIGRDNLILMFTGDHVTPVEIKEHTGDPVPVLLYVPYPIINDNVGDFNEKEARKGSLRIRGLDVTNILLNYSNRAEKYGS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-19" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q980A6</accession>
    <name>RS6E_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein eS6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">30S ribosomal protein S6e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 5" type="primary">rps6e</name>
        <name type="ordered locus">SSO0411</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90185">
        <property type="entry name" value="D90185"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988772.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="G=1-214"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="G=1-214"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="G=1-214"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="G=1-214"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="G=1-214"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="G=1-214"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="G=1-214"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="G=1-214"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q980A6"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q980A6"/>
    <dbReference type="FunCoup" id="Q980A6">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0411"/>
    <dbReference type="PaxDb" id="273057-SSO0411"/>
    <dbReference type="EnsemblBacteria" id="AAK40739">
        <property type="protein sequence ID" value="AAK40739"/>
        <property type="gene ID" value="SSO0411"/>
    </dbReference>
    <dbReference type="GeneID" id="1455549"/>
    <dbReference type="KEGG" id="sso:SSO0411"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.406"/>
    <dbReference type="eggNOG" id="arCOG01946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1275302_0_0_2"/>
    <dbReference type="InParanoid" id="Q980A6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00512">
        <property type="entry name" value="Ribosomal_eS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001377">
        <property type="entry name" value="Ribosomal_eS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020924">
        <property type="entry name" value="Ribosomal_eS6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018282">
        <property type="entry name" value="Ribosomal_eS6_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003292">
        <property type="entry name" value="PRK04290.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11502">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01092">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01405">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00578">
        <property type="entry name" value="RIBOSOMAL_S6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000137361" description="Small ribosomal subunit protein eS6">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="214" mass="23720" checksum="9E20B93EB0247693" modified="2001-10-01" version="1">MPDFKIVISDPQSVEPKRIKVKVKASDQVKSITGEKDGKAVPQAKVNEKTKQLLNVDTLLTLEITKQEGDKKVKVKGHFKVDVDNSVPDNEVWISKTMAEKFGAEDFEAFAYRTKTLQISVDQNKATNLVGLKIGDVFEANQLIGLPVKLKITGGSDNSGFPMRFDVIGAAKRKILLSGPPGFYPNENGERRRKTIRGNTISQEIVQINTIIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q980D5</accession>
    <name>Q980D5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0379</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90181">
        <property type="entry name" value="E90181"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980D5"/>
    <dbReference type="STRING" id="273057.SSO0379"/>
    <dbReference type="PaxDb" id="273057-SSO0379"/>
    <dbReference type="EnsemblBacteria" id="AAK40708">
        <property type="protein sequence ID" value="AAK40708"/>
        <property type="gene ID" value="SSO0379"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0379"/>
    <dbReference type="eggNOG" id="arCOG07273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_549389_0_0_2"/>
    <dbReference type="InParanoid" id="Q980D5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="496" mass="53611" checksum="3AB1DD451F92292A" modified="2001-10-01" version="1">MYLILINIILLSKKFIYKHVLHNVMSVNLRKVLVTGFISLFAILMLILPITLAQSQTISNVLLIPTGFITIPTSGSSASFTAYVINNNPNSETVTVYLNGNSYTTLTIPPYSYSPVTLSLSTGKNILIVNGQSLIINVNSISKVISANILLNNSANLILINGIPGKLYTVNLTIANIHNWNISISTYTYSSIFFTNMYKLYNAPFELQPPLSNSSYIPILVPLYVPQGLYYFYNYVQFYNISNYQQVLGYIPFLIFINVSYGLNGTLVTSNTYTSNNITVSYVTANGYTYILSKYPFSLSDKVIVKVIPHSSSLNPYTVVLGNFTSTLTINGQALQEPYFGSNSSEFAYGNSWIELKIPEQYTGTQYQINITYITPHGIVSTGVIPAITSSTTTTTTTSSTTTTSTTTSTTSTTSTSSSTTSTTTSSSSTTTTSSSITSSSTTSSTTSSTSTTTPTSTSSSTTSVTTSGVSTSVIIGIAIVVIIIVIVAVVLLRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q980D6</accession>
    <name>Q980D6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ornithine cyclodeaminase related protein (Ocd-like1)</fullName>
            <ecNumber evidence="1">4.3.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ocd-like1</name>
        <name evidence="1" type="ordered locus">SSO0378</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.3.1.12" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90181">
        <property type="entry name" value="D90181"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988824.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980D6"/>
    <dbReference type="FunCoup" id="Q980D6">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0378"/>
    <dbReference type="PaxDb" id="273057-SSO0378"/>
    <dbReference type="EnsemblBacteria" id="AAK40707">
        <property type="protein sequence ID" value="AAK40707"/>
        <property type="gene ID" value="SSO0378"/>
    </dbReference>
    <dbReference type="GeneID" id="1455517"/>
    <dbReference type="KEGG" id="sso:SSO0378"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.371"/>
    <dbReference type="eggNOG" id="arCOG01035">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042088_1_2_2"/>
    <dbReference type="InParanoid" id="Q980D6"/>
    <dbReference type="PhylomeDB" id="Q980D6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008473">
        <property type="term" value="F:ornithine cyclodeaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001183">
        <property type="entry name" value="Ornithine cyclodeaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="entry name" value="ornithine cyclodeaminase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812:SF19">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001439">
        <property type="entry name" value="CryM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="309" mass="34090" checksum="6FCE2283BF712A80" modified="2001-10-01" version="1">MIILTRKDLDELLKAEIAVSSVKEAFKLHYQKKVDQPQRLVMSVKGNWWGLMPSSTDYSFVTKIVNVIPENKERGLPLVQGVVILMSPDTGEILAIIDGTTLTAIRTAAASILSTELAINGRNIGILGIIGAGTEAYYHAKLALEYFKVQKLYISARKSHFELAKRVGGEATDLETLLKNSDTIFATTSSNFPVVLGKYLKDVFHLSSIGAHTPDSRELDDDTIIKVKSYIVDSLEAVSKESGDYIIPKQRGLLENKMIKELGEIIEKGIKIERPSLFKTVGIAVEDNITAHIAYQEALRRGIGFNVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q980G1</accession>
    <name>Q980G1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal recognition particle receptor FtsY</fullName>
            <shortName evidence="8">SRP receptor</shortName>
            <ecNumber evidence="8">3.6.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">srpR</name>
        <name evidence="8" type="synonym">ftsY</name>
        <name evidence="10" type="ordered locus">SSO0348</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the GTP-binding SRP family. FtsY subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.4" evidence="8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90177">
        <property type="entry name" value="G90177"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980G1"/>
    <dbReference type="FunCoup" id="Q980G1">
        <property type="interactions" value="138"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0348"/>
    <dbReference type="PaxDb" id="273057-SSO0348"/>
    <dbReference type="EnsemblBacteria" id="AAK40678">
        <property type="protein sequence ID" value="AAK40678"/>
        <property type="gene ID" value="SSO0348"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0348"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.339"/>
    <dbReference type="eggNOG" id="arCOG01227">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_3_4_2"/>
    <dbReference type="InParanoid" id="Q980G1"/>
    <dbReference type="PhylomeDB" id="Q980G1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000053">
        <property type="entry name" value="Signal recognition particle receptor FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF1">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="9">
        <location>
            <begin position="239"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="78"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="160"/>
            <end position="164"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="218"/>
            <end position="221"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK40678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="266" mass="29550" checksum="8B127B1490BC71E7" modified="2001-10-01" version="1">MRYQLLESDVSFEVTEKILEDLKENIIGKKVKRSDDLERIVKDSLKKSITEIITKNNAINVLEEIKKSPKPYIIIFFGINGVGKTTTIAKFAYMLKKNGLSCIISASDTFRAAAQEQLEVHSRNLEIPLIKGRYGGDPASVAFDAIRAAKSRGIDVVLIDTAGRMHTDTDLVNELKRVVNIAKPNLKILVLDSLGGNDALEQAKYFENNVGFDLVILTKVDADVKGGVILSLAYELNKPVGYLGIGQAYDDLIPFNAEWFIQRLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q980M7</accession>
    <name>Q980M7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0264</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90168">
        <property type="entry name" value="D90168"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990536.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980M7"/>
    <dbReference type="STRING" id="273057.SSO0264"/>
    <dbReference type="PaxDb" id="273057-SSO0264"/>
    <dbReference type="EnsemblBacteria" id="AAK40603">
        <property type="protein sequence ID" value="AAK40603"/>
        <property type="gene ID" value="SSO0264"/>
    </dbReference>
    <dbReference type="GeneID" id="1455413"/>
    <dbReference type="KEGG" id="sso:SSO0264"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.259"/>
    <dbReference type="eggNOG" id="arCOG01019">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140136_0_0_2"/>
    <dbReference type="InParanoid" id="Q980M7"/>
    <dbReference type="PhylomeDB" id="Q980M7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018665">
        <property type="entry name" value="DUF2122_RecB-nuclease-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09895">
        <property type="entry name" value="DUF2122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17283" checksum="A455EDD9E0C8C150" modified="2001-10-01" version="1">MKELYFGLHNLTSNQRLLDFSKLAFNVKYVKYLVLTKVGGTAAQSGVPEVNKLAFKYNKPILVLPELKDAIELLKPEITLLVSQNAEKQIDFNDMIKHDKILIVFSGLEGGGFNKIEQSLGEYVRILEDTQDLGAVSLASFFLCKYLQLVEGKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2026-01-28" version="141" xmlns="https://uniprot.org/uniprot">
    <accession>Q980N4</accession>
    <name>CDC61_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ORC1-type DNA replication protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cdc6-1</name>
        <name type="ordered locus">SSO0257</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="Extremophiles" volume="10" first="61" last="70">
            <title>Biochemical characterization of two Cdc6/ORC1-like proteins from the crenarchaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="De Felice M."/>
                <person name="Esposito L."/>
                <person name="Rossi M."/>
                <person name="Pisani F.M."/>
            </authorList>
            <dbReference type="PubMed" id="16179962"/>
            <dbReference type="DOI" id="10.1007/s00792-005-0473-0"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>DNA-BINDING</scope>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH CDC6-3</scope>
        <scope>AUTOPHOSPHORYLATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2006" name="EMBO Rep." volume="7" first="539" last="545">
            <title>GINS, a central nexus in the archaeal DNA replication fork.</title>
            <authorList>
                <person name="Marinsek N."/>
                <person name="Barry E.R."/>
                <person name="Makarova K.S."/>
                <person name="Dionne I."/>
                <person name="Koonin E.V."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="16485022"/>
            <dbReference type="DOI" id="10.1038/sj.embor.7400649"/>
        </citation>
        <scope>INDUCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2007" name="Biochem. Biophys. Res. Commun." volume="361" first="651" last="658">
            <title>Divergent functions of multiple eukaryote-like Orc1/Cdc6 proteins on modulating the loading of the MCM helicase onto the origins of the hyperthermophilic archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Jiang P.X."/>
                <person name="Wang J."/>
                <person name="Feng Y."/>
                <person name="He Z.G."/>
            </authorList>
            <dbReference type="PubMed" id="17673179"/>
            <dbReference type="DOI" id="10.1016/j.bbrc.2007.07.073"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH MCM</scope>
        <scope>DNA-BINDING</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2007" name="Biochem. Biophys. Res. Commun." volume="363" first="63" last="70">
            <title>Three eukaryote-like Orc1/Cdc6 proteins functionally interact and mutually regulate their activities of binding to the replication origin in the hyperthermophilic archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Wang J."/>
                <person name="Jiang P.X."/>
                <person name="Feng H."/>
                <person name="Feng Y."/>
                <person name="He Z.G."/>
            </authorList>
            <dbReference type="PubMed" id="17825793"/>
            <dbReference type="DOI" id="10.1016/j.bbrc.2007.08.125"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH CDC6-3</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2008" name="J. Virol." volume="82" first="4874" last="4883">
            <title>Transcriptome analysis of infection of the archaeon Sulfolobus solfataricus with Sulfolobus turreted icosahedral virus.</title>
            <authorList>
                <person name="Ortmann A.C."/>
                <person name="Brumfield S.K."/>
                <person name="Walther J."/>
                <person name="McInnerney K."/>
                <person name="Brouns S.J."/>
                <person name="van de Werken H.J."/>
                <person name="Bothner B."/>
                <person name="Douglas T."/>
                <person name="van de Oost J."/>
                <person name="Young M.J."/>
            </authorList>
            <dbReference type="PubMed" id="18337566"/>
            <dbReference type="DOI" id="10.1128/jvi.02583-07"/>
        </citation>
        <scope>INDUCTION BY VIRUS (MICROBIAL INFECTION)</scope>
        <source>
            <strain>2-2-12</strain>
        </source>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2009" name="Proc. Natl. Acad. Sci. U.S.A." volume="106" first="7792" last="7797">
            <title>Archaeal eukaryote-like Orc1/Cdc6 initiators physically interact with DNA polymerase B1 and regulate its functions.</title>
            <authorList>
                <person name="Zhang L."/>
                <person name="Zhang L."/>
                <person name="Liu Y."/>
                <person name="Yang S."/>
                <person name="Gao C."/>
                <person name="Gong H."/>
                <person name="Feng Y."/>
                <person name="He Z.G."/>
            </authorList>
            <dbReference type="PubMed" id="19416914"/>
            <dbReference type="DOI" id="10.1073/pnas.0813056106"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH POLB1</scope>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2007" name="Science" volume="317" first="1210" last="1213">
            <title>Replication origin recognition and deformation by a heterodimeric archaeal Orc1 complex.</title>
            <authorList>
                <person name="Dueber E.L."/>
                <person name="Corn J.E."/>
                <person name="Bell S.D."/>
                <person name="Berger J.M."/>
            </authorList>
            <dbReference type="PubMed" id="17761879"/>
            <dbReference type="DOI" id="10.1126/science.1143690"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.35 ANGSTROMS) OF 15-397 IN COMPLEX WITH CDC6-3 AND DNA</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 4 6 8">Involved in regulation of DNA replication. May play essential roles in origin recognition and cell cycle control of replication. Binds to DNA, with a preference for molecules that contain a bubble, a fork, or a tail. Inhibits the binding of the MCM helicase to the origin DNA and strongly inhibits its DNA helicase activity. Also regulates the DNA polymerase and the nuclease activities of PolB1. Stimulates the DNA-binding activity of Cdc6-3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4 5 6 8">Monomer. Interacts with Cdc6-3, MCM and PolB1.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-9026921">
            <id>Q980N4</id>
        </interactant>
        <interactant intactId="EBI-15778666">
            <id>P26811</id>
            <label>dpo1</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>4</experiments>
    </comment>
    <comment type="induction">
        <text evidence="3">Expressed during early, mid- and late-logarithmic growth; expression is maximal in mid-log phase (at protein level).</text>
    </comment>
    <comment type="induction">
        <text evidence="7">(Microbial infection) At least 4-fold induced following infection by Sulfolobus turreted icosahedral virus 1 (STIV-1) in strain 2-2-12.</text>
    </comment>
    <comment type="PTM">
        <text evidence="2">Autophosphorylated in vitro.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">Strain 2-2-12 is a substrain of P2 that is highly susceptible to infection by Sulfolobus turreted icosahedral virus 1 (STIV-1).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90167">
        <property type="entry name" value="E90167"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990521.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2QBY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.35 A"/>
        <property type="chains" value="A=15-397"/>
    </dbReference>
    <dbReference type="PDBsum" id="2QBY"/>
    <dbReference type="AlphaFoldDB" id="Q980N4"/>
    <dbReference type="SMR" id="Q980N4"/>
    <dbReference type="DIP" id="DIP-48851N"/>
    <dbReference type="FunCoup" id="Q980N4">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="IntAct" id="Q980N4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0257"/>
    <dbReference type="PaxDb" id="273057-SSO0257"/>
    <dbReference type="EnsemblBacteria" id="AAK40596">
        <property type="protein sequence ID" value="AAK40596"/>
        <property type="gene ID" value="SSO0257"/>
    </dbReference>
    <dbReference type="GeneID" id="1455406"/>
    <dbReference type="KEGG" id="sso:SSO0257"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.251"/>
    <dbReference type="eggNOG" id="arCOG00467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025112_3_1_2"/>
    <dbReference type="InParanoid" id="Q980N4"/>
    <dbReference type="PhylomeDB" id="Q980N4"/>
    <dbReference type="EvolutionaryTrace" id="Q980N4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08768">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000502">
        <property type="entry name" value="ORC1-type DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000073">
        <property type="entry name" value="ORC1-type DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000930">
        <property type="entry name" value="ORC1-type DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01407">
        <property type="entry name" value="ORC1_type_DNA_replic_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055237">
        <property type="entry name" value="Cdc6_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02928">
        <property type="entry name" value="orc1/cdc6 family replication initiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22703">
        <property type="entry name" value="Cdc6_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151018" description="ORC1-type DNA replication protein 1">
        <location>
            <begin position="1"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="67"/>
            <end position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
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    <name>Q980Q0_SACS2</name>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
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                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
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                <person name="Medina N."/>
                <person name="Peng X."/>
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            <dbReference type="PubMed" id="11427726"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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    <sequence length="582" mass="67649" checksum="8A3141C9E9F6DB37" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
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                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblBacteria" id="AAK40551">
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043528">
        <property type="term" value="C:tRNA (m2G10) methyltransferase complex"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141106">
        <property type="term" value="F:tRNA methyltransferase activator activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000234">
        <property type="term" value="P:positive regulation of rRNA processing"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
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    </dbReference>
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    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158997">
        <property type="entry name" value="Trm112p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="15483" checksum="0DDD119FCDC2020F" modified="2001-10-01" version="1">MKYRLMDLLACPMCKHFPLKLLVFSEREIDRKLSQEDKKPLCELYCTYKSAFTKDLDSLPPCEECIRHEIEEGMLYCESCNRWYPIIDEIPRMLPDKLRKEEDDIKFLEKYKDKIPKSILTNGVPFRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q980T0</accession>
    <name>Q980T0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF460 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90160">
        <property type="entry name" value="H90160"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990437.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980T0"/>
    <dbReference type="STRING" id="273057.SSO0198"/>
    <dbReference type="PaxDb" id="273057-SSO0198"/>
    <dbReference type="EnsemblBacteria" id="AAK40543">
        <property type="protein sequence ID" value="AAK40543"/>
        <property type="gene ID" value="SSO0198"/>
    </dbReference>
    <dbReference type="GeneID" id="1455353"/>
    <dbReference type="KEGG" id="sso:SSO0198"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.197"/>
    <dbReference type="eggNOG" id="arCOG04219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027052_1_0_2"/>
    <dbReference type="InParanoid" id="Q980T0"/>
    <dbReference type="PhylomeDB" id="Q980T0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007408">
        <property type="entry name" value="DUF460"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40707:SF1">
        <property type="entry name" value="DUF460 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40707">
        <property type="entry name" value="POSSIBLE NUCLEASE OF RNASE H FOLD, RUVC/YQGF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04312">
        <property type="entry name" value="DUF460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="417"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="71302" checksum="F1413528D98D9EEA" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-25" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q980U4</accession>
    <name>HEM3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable porphobilinogen deaminase</fullName>
            <shortName evidence="1">PBG</shortName>
            <ecNumber evidence="1">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Hydroxymethylbilane synthase</fullName>
            <shortName evidence="1">HMBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemC</name>
        <name type="ordered locus">SSO0183</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
        <text evidence="1">Binds 1 dipyrromethane group covalently.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The porphobilinogen subunits are added to the dipyrromethane group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90159">
        <property type="entry name" value="B90159"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990418.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980U4"/>
    <dbReference type="SMR" id="Q980U4"/>
    <dbReference type="FunCoup" id="Q980U4">
        <property type="interactions" value="300"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0183"/>
    <dbReference type="PaxDb" id="273057-SSO0183"/>
    <dbReference type="EnsemblBacteria" id="AAK40529">
        <property type="protein sequence ID" value="AAK40529"/>
        <property type="gene ID" value="SSO0183"/>
    </dbReference>
    <dbReference type="GeneID" id="1455339"/>
    <dbReference type="GeneID" id="44129151"/>
    <dbReference type="KEGG" id="sso:SSO0183"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.181"/>
    <dbReference type="eggNOG" id="arCOG04299">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019704_1_0_2"/>
    <dbReference type="InParanoid" id="Q980U4"/>
    <dbReference type="PhylomeDB" id="Q980U4"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00319"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13644">
        <property type="entry name" value="PBP2_HemC_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="entry name" value="Porphobilinogen deaminase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00260">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557:SF0">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000143030" description="Probable porphobilinogen deaminase">
        <location>
            <begin position="1"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="modified residue" description="S-(dipyrrolylmethanemethyl)cysteine" evidence="1">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00260"/>
        </source>
    </evidence>
    <sequence length="293" mass="32773" checksum="BC7E398700151F49" modified="2001-10-01" version="1">MKILIAARGSKLSRVQVDMVGEKLRKIGIEYDVIDIKTKADLFSTEPLSKLGKGVFEKEVNEAVLEGKADVAVHSMKDILSEINPELEIFAVIERDPPYDILIAEKNLHKLDPNTTIGTSSIRRKNFIKYIRPEINTKDIRGNVDTRIRKYLSKEYDGLILAEASIKRLNISISYHRLNVYDFTPEANQGIIVVLGRKKDEKVKEVFKEITHKDTLDEALAERATISVVGGGCHSPIGVLFRKEGKEFYGIASYSDGKKKITVSISKAGDPYTIGSELGLLLKKEMKNEGIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="142" xmlns="https://uniprot.org/uniprot">
    <accession>Q980W0</accession>
    <name>Q980W0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Methyl coenzyme M reductase system, component A2 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO0167</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90157">
        <property type="entry name" value="A90157"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990391.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980W0"/>
    <dbReference type="SMR" id="Q980W0"/>
    <dbReference type="STRING" id="273057.SSO0167"/>
    <dbReference type="PaxDb" id="273057-SSO0167"/>
    <dbReference type="EnsemblBacteria" id="AAK40512">
        <property type="protein sequence ID" value="AAK40512"/>
        <property type="gene ID" value="SSO0167"/>
    </dbReference>
    <dbReference type="GeneID" id="1453339"/>
    <dbReference type="KEGG" id="sso:SSO0167"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.163"/>
    <dbReference type="eggNOG" id="arCOG00188">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_749282_0_0_2"/>
    <dbReference type="InParanoid" id="Q980W0"/>
    <dbReference type="PhylomeDB" id="Q980W0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050095">
        <property type="entry name" value="ECF_ABC_transporter_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553:SF27">
        <property type="entry name" value="ENERGY-COUPLING FACTOR TRANSPORTER ATP-BINDING PROTEIN ECFA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553">
        <property type="entry name" value="HEAVY METAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK40512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="373" mass="42339" checksum="F31E54ADA5C4080A" modified="2001-10-01" version="1">MLIISPKYLNGKIEIEKGERVGLVGKNGSGKTTLILSALCLDQTTSNVTVDDLDFCKEKDYSKISAVLQDVSSQILASTLKDEIELMKRFHSVDDKIARNLMGHYYEVEFNKLSDGYKKRYAIASVLASNPEYILLDEPFANLDDKGVKSVSTIVPKNSLIAEHRIEELRKLVDRVYMIRNNKEINEISKEKLFDIEFLRKEGLRGFSLPKINSELGNEILNVNIDNFTIKIREGEILCLLGRNGSGKTTILRKLSKKIFVIFQEVDLQFFYFTVREIVRSKRVLSLFKLENLADKSPYALSFGQKMRILIASAFSSDSRVIGLDEPSVGMDGEALLSFYEMVKMLKEQKRGLILATHDKDIISLCDTRIVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q981C5</accession>
    <name>Q981C5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90141">
        <property type="entry name" value="D90141"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989625.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981C5"/>
    <dbReference type="PaxDb" id="273057-SSO0020"/>
    <dbReference type="EnsemblBacteria" id="AAK40387">
        <property type="protein sequence ID" value="AAK40387"/>
        <property type="gene ID" value="SSO0020"/>
    </dbReference>
    <dbReference type="GeneID" id="1455278"/>
    <dbReference type="KEGG" id="sso:SSO0020"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.22"/>
    <dbReference type="eggNOG" id="arCOG01830">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1025340_0_0_2"/>
    <dbReference type="InParanoid" id="Q981C5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="269" mass="31400" checksum="2B6ECCFF126FA9EE" modified="2001-10-01" version="1">MIVEGYFLKYRDVIWSIKGCYHPDGYAVAIPRLFNGVKIKRLNDALEVVRTKFSNLLKYVDEIGFNVPLVPLNESEILDPFNAKIFNNRVREFLRFFNGDVGITGSYLYLGKGNDMDFLSFKPEHYYVLEELRKKGITKPLSTVEESEIETLDYSSFKILKSKRILEGIFEGRGYTFKIVQCEKFGPVREVKEFEGYVRILKSIKPFTIPVKYLVTDNHGNEYILTSFRTRFTELLPNMEIFIKGKILVRDEFNDMDLDIAEIVKLKSF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXB2</accession>
    <name>LEU3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>3-isopropylmalate dehydrogenase</fullName>
            <shortName>3-IPM-DH</shortName>
            <shortName>IMDH</shortName>
            <ecNumber>1.1.1.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Beta-IPM dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">leuB</name>
        <name type="ordered locus">SSO0723</name>
        <name type="ORF">C10_007</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:32271"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.85"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90220">
        <property type="entry name" value="F90220"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991286.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXB2"/>
    <dbReference type="SMR" id="Q9UXB2"/>
    <dbReference type="FunCoup" id="Q9UXB2">
        <property type="interactions" value="294"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0723"/>
    <dbReference type="PaxDb" id="273057-SSO0723"/>
    <dbReference type="EnsemblBacteria" id="AAK41021">
        <property type="protein sequence ID" value="AAK41021"/>
        <property type="gene ID" value="SSO0723"/>
    </dbReference>
    <dbReference type="GeneID" id="1454988"/>
    <dbReference type="KEGG" id="sso:SSO0723"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.721"/>
    <dbReference type="eggNOG" id="arCOG01163">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_1_2"/>
    <dbReference type="InParanoid" id="Q9UXB2"/>
    <dbReference type="PhylomeDB" id="Q9UXB2"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00072"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019298">
        <property type="term" value="P:coenzyme B biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000019">
        <property type="entry name" value="Homoisocitrate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011828">
        <property type="entry name" value="LEU3_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02088">
        <property type="entry name" value="LEU3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000083811" description="3-isopropylmalate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="258"/>
            <end position="270"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="1">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="1">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="336" mass="37089" checksum="FE640CBCC5AC567A" modified="2000-05-01" version="1">MTFRVAVIPGDGIGPELYEGSKRIIAKLIEKYNLDIDLIEVEAGDVALNKYGEALPRHTLDVIEKADMILKGPVGETAMDVVVKLRQMYDMYANIRPAKSLPNVQSKYPNVDLVIVRENTEDLYKGFEFVTSTGVTIAIKVTTEFASKRIVNVALNYALMRRRKVTCVHKANVMRVTDGLFASVCREILKGKVNFEEMYVDAAAANLVRDPTRFDVIVTSNVYGDILSDEASQIAGSLGLAPSANIGDRKSMFEPVHGAAFDIAGKGIANPTAFLLSVSMMLSRMYELSKENKYILASKSLENAIIETYKSGNKLTEDVGGSAKLKDMVDEIYKYM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXF5</accession>
    <name>RFCS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Replication factor C small subunit</fullName>
            <shortName>RFC small subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Clamp loader small subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>SsoRFC small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rfcS</name>
        <name type="ordered locus">SSO0768</name>
        <name type="ORF">C40_004</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2000" name="J. Mol. Biol." volume="301" first="61" last="73">
            <title>Biochemical characterization of a clamp-loader complex homologous to eukaryotic replication factor C from the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Pisani F.M."/>
                <person name="De Felice M."/>
                <person name="Carpentieri F."/>
                <person name="Rossi M."/>
            </authorList>
            <dbReference type="PubMed" id="10926493"/>
            <dbReference type="DOI" id="10.1006/jmbi.2000.3964"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2003" name="Mol. Cell" volume="11" first="275" last="282">
            <title>A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Dionne I."/>
                <person name="Nookala R.K."/>
                <person name="Jackson S.P."/>
                <person name="Doherty A.J."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="12535540"/>
            <dbReference type="DOI" id="10.1016/S1097-2765(02)00824-9"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH PCNA1 AND PCNA2</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-dependent ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3">Heteropentamer composed of four small subunits (RfcS) and one large subunit (RfcL). A homotetramer of this subunit interacts with PCNA heterodimer PCNA1-PCNA2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the activator 1 small subunits family. RfcS subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90226">
        <property type="entry name" value="B90226"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991357.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXF5"/>
    <dbReference type="SMR" id="Q9UXF5"/>
    <dbReference type="FunCoup" id="Q9UXF5">
        <property type="interactions" value="150"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0768"/>
    <dbReference type="PaxDb" id="273057-SSO0768"/>
    <dbReference type="EnsemblBacteria" id="AAK41065">
        <property type="protein sequence ID" value="AAK41065"/>
        <property type="gene ID" value="SSO0768"/>
    </dbReference>
    <dbReference type="GeneID" id="1455032"/>
    <dbReference type="KEGG" id="sso:SSO0768"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.767"/>
    <dbReference type="eggNOG" id="arCOG00469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042324_2_1_2"/>
    <dbReference type="InParanoid" id="Q9UXF5"/>
    <dbReference type="PhylomeDB" id="Q9UXF5"/>
    <dbReference type="BRENDA" id="3.6.4.B8">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000029">
        <property type="entry name" value="Replication factor C small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000129">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01509">
        <property type="entry name" value="RfcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023748">
        <property type="entry name" value="Rep_factor-C_ssu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135984" description="Replication factor C small subunit">
        <location>
            <begin position="1"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="48"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10926493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12535540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="330" mass="37741" checksum="89258D375BEEA23E" modified="2000-05-01" version="1">MSTKVEEILWAEKYRPKTLDDIVNQREIIDRLKKFVKEKNMPHLLFAGPPGTGKTTAALALVHDLYGDNYTEYFLELNASDERGIDVIRNKVKEFARTVIPGDIPFKVVLLDEADNMTADAQQALRRTMELYTENTRFILACNYLSKIIEPIQSRTALFRFYPLKKEDVVNRLIYIAKNEKAEYDQKALETIYDITMGDMRKSINILQAASAYGKISVEAVFKVLGLAQPKEVREMINLALQGKFTQARDKLRTLLITYGLSGEDIVKQIHREITSSEIQISEELRVLLLDYIGETEFRIIEGADDEIQLSALLAKMAIYGNKYIGSENR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2026-01-28" version="147" xmlns="https://uniprot.org/uniprot">
    <accession>P50389</accession>
    <name>PNPH_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Purine nucleoside phosphorylase</fullName>
            <shortName>PNP</shortName>
            <ecNumber evidence="2">2.4.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">5'-methylthioadenosine phosphorylase I</fullName>
            <shortName>MTA phosphorylase I</shortName>
            <shortName>MTAPI</shortName>
            <ecNumber evidence="2 3">2.4.2.28</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2706</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Eur. J. Biochem." volume="239" first="632" last="637">
            <title>Extremely thermophilic and thermostable 5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus. Gene cloning and amino acid sequence determination.</title>
            <authorList>
                <person name="Cacciapuoti G."/>
                <person name="Porcelli M."/>
                <person name="Bertoldo C."/>
                <person name="Fusco S."/>
                <person name="De Rosa M."/>
                <person name="Zappia V."/>
            </authorList>
            <dbReference type="PubMed" id="8774706"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1996.0632u.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1994" name="J. Biol. Chem." volume="269" first="24762" last="24769">
            <title>Purification and characterization of extremely thermophilic and thermostable 5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus. Purine nucleoside phosphorylase activity and evidence for intersubunit disulfide bonds.</title>
            <authorList>
                <person name="Cacciapuoti G."/>
                <person name="Porcelli M."/>
                <person name="Bertoldo C."/>
                <person name="de Rosa M."/>
                <person name="Zappia V."/>
            </authorList>
            <dbReference type="PubMed" id="7929153"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-26</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2005" name="FEBS J." volume="272" first="1886" last="1899">
            <title>A novel hyperthermostable 5'-deoxy-5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Cacciapuoti G."/>
                <person name="Forte S."/>
                <person name="Moretti M.A."/>
                <person name="Brio A."/>
                <person name="Zappia V."/>
                <person name="Porcelli M."/>
            </authorList>
            <dbReference type="PubMed" id="15819883"/>
            <dbReference type="DOI" id="10.1111/j.1742-4658.2005.04619.x"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2001" name="J. Biol. Chem." volume="276" first="39232" last="39242">
            <title>Three-dimensional structure of a hyperthermophilic 5'-deoxy-5'-methylthioadenosine phosphorylase from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Appleby T.C."/>
                <person name="Mathews I.I."/>
                <person name="Porcelli M."/>
                <person name="Cacciapuoti G."/>
                <person name="Ealick S.E."/>
            </authorList>
            <dbReference type="PubMed" id="11489901"/>
            <dbReference type="DOI" id="10.1074/jbc.M105694200"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH SUBSTRATES AND SUBSTRATE ANALOGS</scope>
        <scope>DISULFIDE BOND</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules. Cleaves inosine, guanosine, and adenosine with a better efficiency than MTA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>S-methyl-5'-thioadenosine + phosphate = 5-(methylsulfanyl)-alpha-D-ribose 1-phosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:11852"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
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            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.28"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>a purine D-ribonucleoside + phosphate = a purine nucleobase + alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19805"/>
            <dbReference type="ChEBI" id="CHEBI:26386"/>
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        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a purine 2'-deoxy-D-ribonucleoside + phosphate = a purine nucleobase + 2-deoxy-alpha-D-ribose 1-phosphate</text>
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    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">154 uM for S-methyl-5'-thioadenosine</KM>
            <KM evidence="2">25.4 uM for adenosine</KM>
            <KM evidence="2">84 uM for inosine</KM>
            <KM evidence="2">113.6 uM for guanosine</KM>
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        <temperatureDependence>
            <text evidence="2">Optimum temperature is 120 degrees Celsius. Highly thermostable.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 3">Homohexamer; disulfide-linked. Trimer of homodimers, with three symmetric intersubunit disulfide bonds linking the dimers to one another.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PNP/UDP phosphorylase family.</text>
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    <dbReference type="GO" id="GO:0017061">
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            <begin position="1"/>
            <end position="236"/>
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            <position position="5"/>
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        <ligand>
            <name>a purine D-ribonucleoside</name>
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            <note>ligand shared between dimeric partners</note>
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        <location>
            <position position="21"/>
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        <ligand>
            <name>phosphate</name>
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            <note>ligand shared between dimeric partners</note>
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            <name>phosphate</name>
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            <note>ligand shared between dimeric partners</note>
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            <position position="43"/>
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        <ligand>
            <name>phosphate</name>
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            <note>ligand shared between dimeric partners</note>
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    <feature type="binding site" description="in other chain" evidence="1 6 9">
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            <begin position="86"/>
            <end position="89"/>
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        <ligand>
            <name>phosphate</name>
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            <note>ligand shared between dimeric partners</note>
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    <feature type="binding site" description="in other chain" evidence="1">
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            <position position="163"/>
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        <ligand>
            <name>a purine D-ribonucleoside</name>
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            <note>ligand shared between dimeric partners</note>
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            <begin position="180"/>
            <end position="182"/>
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        <ligand>
            <name>a purine D-ribonucleoside</name>
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            <note>ligand shared between dimeric partners</note>
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            <begin position="204"/>
            <end position="205"/>
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        <ligand>
            <name>a purine D-ribonucleoside</name>
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            <note>ligand shared between dimeric partners</note>
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            <begin position="4"/>
            <end position="6"/>
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    <sequence length="236" mass="25738" checksum="F1570ECE8AA3D51B" modified="1996-10-01" version="1">MNPVHILAKKGEVAERVLVVGDPGRARLLSTLLQNPKLTNENRGFLVYTGKYNGETVSIATHGIGGPSIAIVLEELAMLGANVFIRYGTTGALVPYINLGEYIIVTGASYNQGGLFYQYLRDNACVASTPDFELTNKLVTSFSKRNLKYYVGNVFSSDAFYAEDEEFVKKWSSRGNIAVEMECATLFTLSKVKGWKSATVLVVSDNLAKGGIWITKEELEKSVMDGAKAVLDTLTS</sequence>
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<entry dataset="Swiss-Prot" created="2001-04-27" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>P58030</accession>
    <name>CBSB_SACS2</name>
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        <recommendedName>
            <fullName>Cytochrome b558/566 subunit B</fullName>
        </recommendedName>
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    <gene>
        <name type="primary">cbsB</name>
        <name type="ordered locus">SSO2802</name>
        <name type="ORF">C48_010</name>
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    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90457">
        <property type="entry name" value="D90457"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991444.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58030"/>
    <dbReference type="STRING" id="273057.SSO2802"/>
    <dbReference type="PaxDb" id="273057-SSO2802"/>
    <dbReference type="EnsemblBacteria" id="AAK42915">
        <property type="protein sequence ID" value="AAK42915"/>
        <property type="gene ID" value="SSO2802"/>
    </dbReference>
    <dbReference type="GeneID" id="1452834"/>
    <dbReference type="GeneID" id="44128550"/>
    <dbReference type="KEGG" id="sso:SSO2802"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2888"/>
    <dbReference type="eggNOG" id="arCOG06016">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_884600_0_0_2"/>
    <dbReference type="InParanoid" id="P58030"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017573">
        <property type="entry name" value="Cyt_b558/566_suB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03155">
        <property type="entry name" value="sulfolob_CbsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000089386" description="Cytochrome b558/566 subunit B">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="314" mass="35030" checksum="7D14E8F01922ECA4" modified="2001-04-27" version="1">MKMVDELKGNLNSFLILLGIFSFLQFSFKQSFMFPSILPLNIPDSNLLLVIGNISFYFFFVFLLIVSMILSLTYKSLIPLTIILTISPFITLIPNYENSFLLYSLEIAILILGLASTIEGLIKSSLLSILLIPTLVLVNLGIYASILLNIFHNALFISYLTIYLISIAGYLAYVISWGKIKSLRNYIAISVGLLSMIPFIFFENIISHNRYLEILMNMILPSTLGITLYNPYHITLLVMALGLSTMGIVTSLIKGNVSSGVGYFLIITTVFLGIDGFSLLIYMLTPIIGFLVITSSEIESKRRLIDIISPTRNG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-17" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>P95855</accession>
    <accession>Q9HHC3</accession>
    <name>DPS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>DNA protection during starvation protein</fullName>
            <ecNumber>1.16.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">dps</name>
        <name type="ordered locus">SSO2079</name>
        <name type="ORF">C06008</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2002" name="Protein Sci." volume="11" first="2969" last="2973">
            <title>Redox-dependent structural changes in archaeal and bacterial Rieske-type [2Fe-2S] clusters.</title>
            <authorList>
                <person name="Cosper N.J."/>
                <person name="Eby D.M."/>
                <person name="Kounosu A."/>
                <person name="Kurosawa N."/>
                <person name="Neidle E.L."/>
                <person name="Kurtz D.M. Jr."/>
                <person name="Iwasaki T."/>
                <person name="Scott R.A."/>
            </authorList>
            <dbReference type="PubMed" id="12441394"/>
            <dbReference type="DOI" id="10.1110/ps.0222402"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 151-188</scope>
        <source>
            <strain>ATCC 35091 / DSM 1616 / IFO 15331 / JCM 8930 / P1</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2005" name="Proc. Natl. Acad. Sci. U.S.A." volume="102" first="10551" last="10556">
            <title>An archaeal antioxidant: characterization of a Dps-like protein from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Wiedenheft B."/>
                <person name="Mosolf J."/>
                <person name="Willits D."/>
                <person name="Yeager M."/>
                <person name="Dryden K.A."/>
                <person name="Young M."/>
                <person name="Douglas T."/>
            </authorList>
            <dbReference type="PubMed" id="16024730"/>
            <dbReference type="DOI" id="10.1073/pnas.0501497102"/>
        </citation>
        <scope>FUNCTION IN IRON OXIDATION</scope>
        <scope>FUNCTION IN MINERALIZATION</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3">Protects DNA from oxidative damage by sequestering intracellular Fe(2+) ion and storing it in the form of Fe(3+) oxyhydroxide mineral. One hydrogen peroxide oxidizes two Fe(2+) ions, which prevents hydroxyl radical production by the Fenton reaction (By similarity). In vitro, it efficiently oxidizes Fe(2+) in the presence of hydrogen peroxide and stores it as a mineral core.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>2 Fe(2+) + H2O2 + 2 H(+) = 2 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:48712"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homododecamer. The 12 identical subunits form a hollow sphere into which the mineral iron core of up to 300 Fe(3+) can be deposited.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text>Up-regulated in cultures grown in iron-depleted media and upon hydrogen peroxide stress, but is not induced by other stress.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Dps family.</text>
    </comment>
    <dbReference type="EC" id="1.16.-.-"/>
    <dbReference type="EMBL" id="Y08256">
        <property type="protein sequence ID" value="CAA69491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AB047031">
        <property type="protein sequence ID" value="BAB17764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73096">
        <property type="entry name" value="S73096"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989805.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2CLB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B/C/D/M/N/O/P=1-188"/>
    </dbReference>
    <dbReference type="PDBsum" id="2CLB"/>
    <dbReference type="AlphaFoldDB" id="P95855"/>
    <dbReference type="SMR" id="P95855"/>
    <dbReference type="STRING" id="273057.SSO2079"/>
    <dbReference type="PaxDb" id="273057-SSO2079"/>
    <dbReference type="EnsemblBacteria" id="AAK42260">
        <property type="protein sequence ID" value="AAK42260"/>
        <property type="gene ID" value="SSO2079"/>
    </dbReference>
    <dbReference type="GeneID" id="1453587"/>
    <dbReference type="GeneID" id="44130785"/>
    <dbReference type="KEGG" id="sso:SSO2079"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2157"/>
    <dbReference type="eggNOG" id="arCOG01093">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104506_4_0_2"/>
    <dbReference type="InParanoid" id="P95855"/>
    <dbReference type="PhylomeDB" id="P95855"/>
    <dbReference type="EvolutionaryTrace" id="P95855"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009295">
        <property type="term" value="C:nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01052">
        <property type="entry name" value="DPSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053475">
        <property type="entry name" value="DPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014490">
        <property type="entry name" value="Dps-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033921">
        <property type="entry name" value="DPSL_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040955">
        <property type="entry name" value="Arch_DPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009990">
        <property type="entry name" value="PRK13456.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30295">
        <property type="entry name" value="BACTERIOFERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30295:SF1">
        <property type="entry name" value="DNA PROTECTION DURING STARVATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018063">
        <property type="entry name" value="Ferrtn_UCP018063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0409">Iron storage</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000253338" description="DNA protection during starvation protein">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="11"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="18"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="53"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="57"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="92"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="108"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="139"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="16024730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="2CLB"/>
        </source>
    </evidence>
    <sequence length="188" mass="21770" checksum="617F4CA5A2316F6C" modified="1997-05-01" version="1">MQEKPQEPKVVGVEILEKSGLDIKKLVDKLVKATAAEFTTYYYYTILRMHLTGMEGEGLKEIAEDARLEDRLHFELMTQRIYELGGGLPRDIRQLADISACSDAYLPENWKDPKEILKVLLEAEQCAIRTWKEVCDMTYGKDPRTYDLAQRILQEEIEHEAWFLELLYGRPSGHFRRSSPGNAPYSKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-08-29" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>P96000</accession>
    <name>GPT_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Putative UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase</fullName>
            <ecNumber>2.7.8.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>GlcNAc-1-P transferase</fullName>
            <shortName>G1PT</shortName>
            <shortName>GPT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>N-acetylglucosamine-1-phosphate transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">gnpTA</name>
        <name type="ordered locus">SSO0060</name>
        <name type="ORF">C05011</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = an N-acetyl-alpha-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol + UMP</text>
            <dbReference type="Rhea" id="RHEA:13289"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19507"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58427"/>
            <dbReference type="EC" id="2.7.8.15"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Inhibited by tunicamycin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 4 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.15"/>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75428">
        <property type="entry name" value="S75428"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988861.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P96000"/>
    <dbReference type="SMR" id="P96000"/>
    <dbReference type="FunCoup" id="P96000">
        <property type="interactions" value="251"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0060"/>
    <dbReference type="PaxDb" id="273057-SSO0060"/>
    <dbReference type="DNASU" id="1455313"/>
    <dbReference type="EnsemblBacteria" id="AAK40422">
        <property type="protein sequence ID" value="AAK40422"/>
        <property type="gene ID" value="SSO0060"/>
    </dbReference>
    <dbReference type="GeneID" id="1455313"/>
    <dbReference type="KEGG" id="sso:SSO0060"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.61"/>
    <dbReference type="eggNOG" id="arCOG03199">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023982_4_0_2"/>
    <dbReference type="InParanoid" id="P96000"/>
    <dbReference type="PhylomeDB" id="P96000"/>
    <dbReference type="PRO" id="PR:P96000"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003975">
        <property type="term" value="F:UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044038">
        <property type="term" value="P:cell wall macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06856">
        <property type="entry name" value="GT_GPT_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PHOSPHO-N-ACETYLMURAMOYL-PENTAPEPTIDE-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926:SF3">
        <property type="entry name" value="UNDECAPRENYL-PHOSPHATE ALPHA-N-ACETYLGLUCOSAMINYL 1-PHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000108767" description="Putative UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase">
        <location>
            <begin position="1"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="322" mass="35252" checksum="41F58B03A3269F7D" modified="1997-05-01" version="1">MRILAILLPILISFFISYITTVWVIRQAKKSRFVGKDINKPDKPEIPLLGGIGIIAGFIAGSFSLLLTDVRSERVIPAVILSSLLIAFLGLLDDIFNVRQSVRAFLPIFASVPLIVYSVGHSIISIPFLGPINFGIFYYIIIIPFALTITSNAFNMLEGLNGLGVGMGIIMLSALAYIGLTHTGPTYQAGLIALSAIFSLSAFLIFNKYPAKIFPGNVGTYFIGALIGAIGIAGFMYTALAILYIPYVVEFILKLRTNFKGVSFGKVDSSGRLYWDEKPHSLTHIVMKMGRFKEYQVVIILWGMEAIFAVIAVILQTTTIVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1994-06-01" modified="2026-01-28" version="156" xmlns="https://uniprot.org/uniprot">
    <accession>Q06121</accession>
    <accession>P50385</accession>
    <name>TRPC_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Indole-3-glycerol phosphate synthase</fullName>
            <shortName>IGPS</shortName>
            <ecNumber>4.1.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">trpC</name>
        <name type="ordered locus">SSO0895</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1993" name="J. Bacteriol." volume="175" first="299" last="302">
            <title>Tryptophan biosynthesis genes trpEGC in the thermoacidophilic archaebacterium Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Tutino M.L."/>
                <person name="Scarano G."/>
                <person name="Marino G."/>
                <person name="Sannia G."/>
                <person name="Cubellis M.V."/>
            </authorList>
            <dbReference type="PubMed" id="8416906"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="1995-07" db="EMBL/GenBank/DDBJ databases">
            <title>The tryptophan operon in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Tutino M.L."/>
                <person name="Cubellis M."/>
                <person name="Sannia G."/>
                <person name="Marino G."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="1995" name="Structure" volume="3" first="1295" last="1306">
            <title>2.0-A structure of indole-3-glycerol phosphate synthase from the hyperthermophile Sulfolobus solfataricus: possible determinants of protein stability.</title>
            <authorList>
                <person name="Hennig M."/>
                <person name="Darimont B."/>
                <person name="Kirschner K."/>
                <person name="Jansonius J.N."/>
            </authorList>
            <dbReference type="PubMed" id="8747456"/>
            <dbReference type="DOI" id="10.1016/S0969-2126(01)00267-2"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="1996" name="J. Mol. Biol." volume="262" first="502" last="515">
            <title>The crystal structure of indole-3-glycerol phosphate synthase from the hyperthermophilic archaeon Sulfolobus solfataricus in three different crystal forms: effects of ionic strength.</title>
            <authorList>
                <person name="Knoechel T.R."/>
                <person name="Hennig M."/>
                <person name="Merz A."/>
                <person name="Darimont B."/>
                <person name="Kirschner K."/>
                <person name="Jansonius J.N."/>
            </authorList>
            <dbReference type="PubMed" id="8893859"/>
            <dbReference type="DOI" id="10.1006/jmbi.1996.0531"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)</scope>
    </reference>
    <reference key="6">
        <citation type="submission" date="1998-02" db="PDB data bank">
            <title>The catalytic mechanism of indole-3-glycerolphosphate synthase: crystal structure of substrate, product and substrate analogue bound to the enzyme from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Hennig M."/>
                <person name="Darimont B."/>
                <person name="Kirschner K."/>
                <person name="Jansonius J.N."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text>Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48"/>
    <dbReference type="EMBL" id="M98048">
        <property type="protein sequence ID" value="AAA73381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Z50014">
        <property type="protein sequence ID" value="CAA90313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C40635">
        <property type="entry name" value="C40635"/>
    </dbReference>
    <dbReference type="PIR" id="S50179">
        <property type="entry name" value="S50179"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992313.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1A53">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=2-248"/>
    </dbReference>
    <dbReference type="PDB" id="1IGS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-248"/>
    </dbReference>
    <dbReference type="PDB" id="1JUK">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A=1-248"/>
    </dbReference>
    <dbReference type="PDB" id="1JUL">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-248"/>
    </dbReference>
    <dbReference type="PDB" id="1LBF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.05 A"/>
        <property type="chains" value="A=2-248"/>
    </dbReference>
    <dbReference type="PDB" id="1LBL">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A=2-248"/>
    </dbReference>
    <dbReference type="PDB" id="2C3Z">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A=27-248"/>
    </dbReference>
    <dbReference type="PDB" id="3NYZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.51 A"/>
        <property type="chains" value="A/B=1-248"/>
    </dbReference>
    <dbReference type="PDB" id="3NZ1">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.56 A"/>
        <property type="chains" value="A=1-248"/>
    </dbReference>
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<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="63" xmlns="https://uniprot.org/uniprot">
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    <name>Q7LX34_SACS2</name>
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            <taxon>Saccharolobus</taxon>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
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            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
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                <person name="De Moors A."/>
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                <person name="Gordon P.M.K."/>
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                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="4">P2</strain>
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    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="299" mass="34854" checksum="3E78259A5C2AD6EF" modified="2011-05-31" version="1">MEKSKYKRDWSKYDENVITRYKLMFPFYVFEHWWDLLAEENRNARTKYKAPKEFDEFLAFLHIFLPYRAIEGVLRALEQLKIIPTSLDYSTIWKRVRNMKITFPEASDELEVIADATGISTNTGGQYIVAKWGKTRDSKFLKIEIVMDNNEFNVINAEVTSNEVESAVKTVKDLQDKGKKVKKFYGDKTYDANEVYKTGVEVVVPPKKNASTKRGHLARRKAVREFRKLGYDRWREEKGYGVRWRVESLFSAVKRTFGESVRATSFAGQVVEAKLKFWAYAWMVHLANSVVGRAPGIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX97</accession>
    <name>Q7LX97_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2096</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73090">
        <property type="entry name" value="S73090"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989778.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX97"/>
    <dbReference type="STRING" id="273057.SSO2096"/>
    <dbReference type="PaxDb" id="273057-SSO2096"/>
    <dbReference type="EnsemblBacteria" id="AAK42275">
        <property type="protein sequence ID" value="AAK42275"/>
        <property type="gene ID" value="SSO2096"/>
    </dbReference>
    <dbReference type="GeneID" id="1453602"/>
    <dbReference type="KEGG" id="sso:SSO2096"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2176"/>
    <dbReference type="eggNOG" id="arCOG02219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118482_3_2_2"/>
    <dbReference type="InParanoid" id="Q7LX97"/>
    <dbReference type="PhylomeDB" id="Q7LX97"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000103">
        <property type="entry name" value="PilT protein domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051749">
        <property type="entry name" value="PINc/VapC_TA_RNase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42740">
        <property type="entry name" value="RIBONUCLEASE VAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42740:SF1">
        <property type="entry name" value="RIBONUCLEASE VAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="domain" description="PIN" evidence="6">
        <location>
            <begin position="6"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="123" mass="14371" checksum="D82F9206A535EE1E" modified="2011-05-31" version="1">MENKRICLDTDVLIDAFRNDIKKFIGYYTTCINLYEFLRGLAFIGKNIDEFKSWIELNLNVVCIDNNSLKTASRIYAELRKMGEIIEDPDLLIASICIANDFSLMTHNKKHFKRLEKYGLKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXA4</accession>
    <name>Q7LXA4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiaminase-2/PQQC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73084">
        <property type="entry name" value="S73084"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989794.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXA4"/>
    <dbReference type="STRING" id="273057.SSO2089"/>
    <dbReference type="PaxDb" id="273057-SSO2089"/>
    <dbReference type="EnsemblBacteria" id="AAK42268">
        <property type="protein sequence ID" value="AAK42268"/>
        <property type="gene ID" value="SSO2089"/>
    </dbReference>
    <dbReference type="GeneID" id="1453595"/>
    <dbReference type="KEGG" id="sso:SSO2089"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2167"/>
    <dbReference type="eggNOG" id="arCOG01129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108435_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXA4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiaminase-2/PQQC" evidence="1">
        <location>
            <begin position="9"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="206" mass="24090" checksum="EA47562DEA3829B3" modified="2011-05-31" version="1">MNVLEQIRKELEGLNDQIINHPLLKEKELKKEVVHSFVINQWYIVNHDLRSLAIGLSRSTNLEELDIFKILVDGDYHALKEIVKLMNELGIEVKDPLLYNVSPPAVSYTHYLSWLASYAKPSEFLFAGIVNLPVWASVVTRFGDMIKEKFNIRETGFFEAFRGSYKELEDRIVRLVENSQVERLRGIAYTIQYYEKSFWDSVYTTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-02-05" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXE3</accession>
    <name>Q7LXE3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial ORF from ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90234">
        <property type="entry name" value="C90234"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXE3"/>
    <dbReference type="STRING" id="273057.SSO0836"/>
    <dbReference type="PaxDb" id="273057-SSO0836"/>
    <dbReference type="EnsemblBacteria" id="AAK41130">
        <property type="protein sequence ID" value="AAK41130"/>
        <property type="gene ID" value="SSO0836"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0836"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176875_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LXE3"/>
    <dbReference type="PhylomeDB" id="Q7LXE3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="19"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="100" mass="11902" checksum="0D6B5872B6EC605E" modified="2011-05-31" version="1">MRFKEEDVERLMGIIRKRKVILYARVSSNKDDLINQVKYSRRTSKITTKSSPFWIKHEEKRFLKLLGMILDNEVSKVVITYPDRLVRFGLEYPKHTTANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXF1</accession>
    <name>Q7LXF1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C99233">
        <property type="entry name" value="C99233"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923117.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXF1"/>
    <dbReference type="STRING" id="273057.SSO0827"/>
    <dbReference type="PaxDb" id="273057-SSO0827"/>
    <dbReference type="EnsemblBacteria" id="AAK41122">
        <property type="protein sequence ID" value="AAK41122"/>
        <property type="gene ID" value="SSO0827"/>
    </dbReference>
    <dbReference type="GeneID" id="1455089"/>
    <dbReference type="KEGG" id="sso:SSO0827"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.839"/>
    <dbReference type="eggNOG" id="arCOG01398">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1736499_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXF1"/>
    <dbReference type="PhylomeDB" id="Q7LXF1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="15"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="150" mass="17777" checksum="C2D74AD915BC65A9" modified="2011-05-31" version="1">MRRWLKTLLFTRINTKAIVANFDQAEISRYLCNKWKFIQSKNRWIGPKVSEALKETSGDVVVFLEDDDLFERTKLKVIYDIFKQNQNLGFYRHKVKIINENGKEANLPYNLYNSGKIVIKKSRNIGKQYLLTYTEAVMCPCLPWQSEEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-03-27" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXQ4</accession>
    <name>Q7LXQ4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Adenosine monophosphate-protein transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0651</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90212">
        <property type="entry name" value="E90212"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXQ4"/>
    <dbReference type="STRING" id="273057.SSO0651"/>
    <dbReference type="PaxDb" id="273057-SSO0651"/>
    <dbReference type="EnsemblBacteria" id="AAK40956">
        <property type="protein sequence ID" value="AAK40956"/>
        <property type="gene ID" value="SSO0651"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0651"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.654"/>
    <dbReference type="eggNOG" id="arCOG04298">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138382_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXQ4"/>
    <dbReference type="PhylomeDB" id="Q7LXQ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1520.10">
        <property type="entry name" value="Ta1353-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007153">
        <property type="entry name" value="Adenosine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036902">
        <property type="entry name" value="Ta1353-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36155">
        <property type="entry name" value="BLL5354 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36155:SF1">
        <property type="entry name" value="BLL5354 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04008">
        <property type="entry name" value="Adenosine_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103165">
        <property type="entry name" value="Ta1353-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="18162" checksum="9E38204618FFB5D5" modified="2011-05-31" version="1">MMSIKIDVVRIDIPEGTNVIIGQSHFIKTVEDLYETLSSSSPNLKFGIAFNEASSKRLIRYDGNDQELIKLAIENAKKIGAGHVFVIYLKNGYPINVLNRIKNVEEVVRIFVATANPLQVIVAETDQGRGVIGVVDGYAPLGVESEADIQERKEFLRKIGYKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXR2</accession>
    <name>Q7LXR2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="2 10">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GARS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 10">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 10">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">purD</name>
        <name evidence="13" type="ordered locus">SSO0635</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-phospho-beta-D-ribosylamine + glycine + ATP = N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 10">Belongs to the GARS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="2 10"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90210">
        <property type="entry name" value="H90210"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXR2"/>
    <dbReference type="FunCoup" id="Q7LXR2">
        <property type="interactions" value="152"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0635"/>
    <dbReference type="PaxDb" id="273057-SSO0635"/>
    <dbReference type="EnsemblBacteria" id="AAK40943">
        <property type="protein sequence ID" value="AAK40943"/>
        <property type="gene ID" value="SSO0635"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0635"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.639"/>
    <dbReference type="eggNOG" id="arCOG04415">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027420_3_0_2"/>
    <dbReference type="InParanoid" id="Q7LXR2"/>
    <dbReference type="PhylomeDB" id="Q7LXR2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="entry name" value="Phosphoribosylglycinamide synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01209">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="118"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAK40943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="483" mass="53333" checksum="6DB51AB10779AA60" modified="2011-05-31" version="1">MVRKMKVLLVGDGARENVLAYSLARSSKGYKIYALSSYINPGINSIVKTTGAEYFIGNVNSPEVIKEVIKKVNPDLGVIGPEDPLFNGIADIFRKEGISVFGASKKCARIEESKAWARELMWKHSIPGRLRYKVFYTIEDTAKFILEYGGSVAIKPAGQAGGKGVKVIADLEAYLTHDKREALTKSVNEIGSLYNKEGEPRIIIEEKVDGPEYTLHVLSDGKTTISLPLAQDYKNAYQDGIGPETGGMGSISGPNELLPFISNEEYQTTYDIVKRTMDAIYKETGERYVGVIAGQMMLTELWGPTVIEYYSRFGDPEASAIIPRLESDFGETIELTATGHLNKASIKINEKPSIVRAVATLGYPISKQMASGHKIVVDLEKMKERGCVVFFGSVALEGMQLITKGSRALEIVAIGDFEEAAENLDRCMQYISSDTKLIYRTDIGRTVKSQIEKAEIIRYSYKNREKRGILGVSADWSPNGGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXW0</accession>
    <name>Q7LXW0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0538</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90200">
        <property type="entry name" value="B90200"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991037.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXW0"/>
    <dbReference type="PaxDb" id="273057-SSO0538"/>
    <dbReference type="EnsemblBacteria" id="AAK40857">
        <property type="protein sequence ID" value="AAK40857"/>
        <property type="gene ID" value="SSO0538"/>
    </dbReference>
    <dbReference type="GeneID" id="1454824"/>
    <dbReference type="KEGG" id="sso:SSO0538"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.537"/>
    <dbReference type="eggNOG" id="arCOG07283">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128033_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXW0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="180" mass="20839" checksum="D3A4DCB80FDAB01F" modified="2011-05-31" version="1">MEEKRLSFFKWLGLALLFIVLPSAVAVVLSFSVPYYILHDMTLANALSTIIFILGFGVSAIYFNRYLESRGLITPFMKRVSITILPDSGQPIDEKYIKSFEARLKFAKGEEYIKLLAMLGMMYLQNAVAYDNKDFYLRAKEYLSRAEEAMQEKSVSFETKALVDNLRSKIETYKYRFGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LY03</accession>
    <name>Q7LY03_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75431">
        <property type="entry name" value="S75431"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988859.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LY03"/>
    <dbReference type="STRING" id="273057.SSO0057"/>
    <dbReference type="PaxDb" id="273057-SSO0057"/>
    <dbReference type="EnsemblBacteria" id="AAK40421">
        <property type="protein sequence ID" value="AAK40421"/>
        <property type="gene ID" value="SSO0057"/>
    </dbReference>
    <dbReference type="GeneID" id="1455312"/>
    <dbReference type="KEGG" id="sso:SSO0057"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.60"/>
    <dbReference type="eggNOG" id="arCOG06952">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1773286_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LY03"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="148" mass="17789" checksum="40E0886853032D74" modified="2011-05-31" version="1">MTKDIVKKLVINSMKRVKIGIFYLENDMIEEARKEIQKSWRQIMSALLILNLYNAEEKQFTHYNVPRSKIFSVSKSLEDKGYRDLIKISSMYFSSLQELPKSEIKILITVFIKEEIEHIKEWFKAIWDEKLDDELNEIKKEKQWLIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LYK0</accession>
    <name>Q7LYK0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S43883">
        <property type="entry name" value="S43883"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923767.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LYK0"/>
    <dbReference type="STRING" id="273057.SSO2077"/>
    <dbReference type="PaxDb" id="273057-SSO2077"/>
    <dbReference type="EnsemblBacteria" id="AAK42258">
        <property type="protein sequence ID" value="AAK42258"/>
        <property type="gene ID" value="SSO2077"/>
    </dbReference>
    <dbReference type="GeneID" id="1453585"/>
    <dbReference type="KEGG" id="sso:SSO2077"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2155"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077283_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LYK0"/>
    <dbReference type="PhylomeDB" id="Q7LYK0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="354" mass="40276" checksum="8769A62E1EAF6952" modified="2011-05-31" version="1">MTKELTREEYYKALEKAVNEVILSMTGTRKDVAKRLLLGAVVGRNATEIAQEAEMDYETVLNNLDKAAQAKLIEVVKKLVGDHPVLLIVDDTHDHKLYARAMPVSRNGAQIFYCRAHKRFEPAIQLLVIGVKDLVNNQIYVIHIIAYIPRKVEEELKRRGEEVKFKTKIDALLEFLSSLSGLNVKARVFDSWYVNSRTLQGNTVGELKSSARVVEGGRSVPVSEFPQGEYLVEYLGTPIKLLVIDDYKGYGRRYFFSTDLNDTAEDIITTWENRWDIEVLIRELKALGLEGGSFLTWVRNSGFVALKALSLLVVQYFKYSTGLMLGAKRLARLIKSIYREAGGIKKLFKRRRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U08</accession>
    <name>Q97U08_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO12210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90507">
        <property type="entry name" value="D90507"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U08"/>
    <dbReference type="PaxDb" id="273057-SSO12210"/>
    <dbReference type="EnsemblBacteria" id="AAK43315">
        <property type="protein sequence ID" value="AAK43315"/>
        <property type="gene ID" value="SSO12210"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO12210"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3322"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2784299_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U08"/>
    <dbReference type="PhylomeDB" id="Q97U08"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="68" mass="8405" checksum="9671DF999EA7CBDA" modified="2001-10-01" version="1">MKLACKIYNTLRWADIYFYQRDGKGLTQTELRQLALDLRKQDDEYKQLYSQVVQQIADRYYEARQRFF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-01-25" modified="2026-01-28" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U21</accession>
    <name>GLCD3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucose 1-dehydrogenase 3</fullName>
            <shortName evidence="1">GDH 3</shortName>
            <shortName evidence="1">GlcDH 3</shortName>
            <ecNumber evidence="1">1.1.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gdh3</name>
        <name type="synonym">dhg-3</name>
        <name type="ordered locus">SSO3204</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NAD(P)(+)-dependent oxidation of D-glucose to D-gluconate via gluconolactone. Can utilize both NAD(+) and NADP(+) as electron acceptor. Is involved in the degradation of glucose through a non-phosphorylative variant of the Entner-Doudoroff pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose + NAD(+) = D-glucono-1,5-lactone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14293"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16217"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.47"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose + NADP(+) = D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14405"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16217"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit. One of the zinc atoms is essential for catalytic activity while the other has a structural function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family. Glucose 1-dehydrogenase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.47" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90505">
        <property type="entry name" value="F90505"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991676.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U21"/>
    <dbReference type="SMR" id="Q97U21"/>
    <dbReference type="FunCoup" id="Q97U21">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3204"/>
    <dbReference type="PaxDb" id="273057-SSO3204"/>
    <dbReference type="EnsemblBacteria" id="AAK43301">
        <property type="protein sequence ID" value="AAK43301"/>
        <property type="gene ID" value="SSO3204"/>
    </dbReference>
    <dbReference type="GeneID" id="1453220"/>
    <dbReference type="KEGG" id="sso:SSO3204"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3306"/>
    <dbReference type="eggNOG" id="arCOG01459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_1_0_2"/>
    <dbReference type="InParanoid" id="Q97U21"/>
    <dbReference type="PhylomeDB" id="Q97U21"/>
    <dbReference type="BRENDA" id="1.1.1.47">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047934">
        <property type="term" value="F:glucose 1-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047935">
        <property type="term" value="F:glucose 1-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019595">
        <property type="term" value="P:non-phosphorylated glucose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08230">
        <property type="entry name" value="glucose_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02127">
        <property type="entry name" value="Glucose_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026583">
        <property type="entry name" value="Glc_1-DH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031640">
        <property type="entry name" value="Glu_dehyd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF2">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16912">
        <property type="entry name" value="Glu_dehyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000414842" description="Glucose 1-dehydrogenase 3">
        <location>
            <begin position="1"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="192"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="214"/>
            <end position="216"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="274"/>
            <end position="276"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="302"/>
            <end position="304"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
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        </location>
        <ligand>
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        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02127"/>
        </source>
    </evidence>
    <sequence length="360" mass="39970" checksum="84D0C39C4DB1E878" modified="2001-10-01" version="1">MKAIVVNPPNKGVHVKEINDIHRSLTADEVLVKTIANGICGTDRGIVSGLLKFSRPPNGKNDLVLGHENLGQVIDKGPEVHGLGKGDYVVSIVRRGCGKCSNCLAGRQDFCETGEFVEAGIRGLDGFMREFYIDNASYLVKIPDEIVDIAVLLEPLSNVVKAYSELMLVQRRMTWWCKDGSYNCRNVAIVGSGPIGLMFSLMFSIQGFNAFVLNKRDPFPIEAEIVEKSNAKFINTNKDRLPNTIDLLIDTSGYPSAFIPLMSRLNKNSAIILFGTTGGEKFEVNADLITYLVENNILLFGSVNASKKDFENGVNYLTIWKYRYPSVLNRMITRVIKPEQAPEVLYTKPKGEIKTVISWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U67</accession>
    <name>Q97U67_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">First ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO3154</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="H90499"/>
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    <dbReference type="RefSeq" id="WP_010924149.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="PaxDb" id="273057-SSO3154"/>
    <dbReference type="EnsemblBacteria" id="AAK43255">
        <property type="protein sequence ID" value="AAK43255"/>
        <property type="gene ID" value="SSO3154"/>
    </dbReference>
    <dbReference type="GeneID" id="1453174"/>
    <dbReference type="KEGG" id="sso:SSO3154"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3260"/>
    <dbReference type="eggNOG" id="arCOG03164">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="InParanoid" id="Q97U67"/>
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        <property type="component" value="Chromosome"/>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:DNA strand exchange activity"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="HTH_MerR-trunc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1390:FF:000002">
        <property type="entry name" value="ORF1 in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="5">
        <location>
            <begin position="55"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="192" mass="22423" checksum="EEB3269812D33E6F" modified="2001-10-01" version="1">MLRPKEACQRLGISYATLREYVKKGYIKPVILQSGKWRFREEDIERLMGIIRKRKVILYARVSSSTQKDDLVNQVKYLEEQVKEYDLVITDIGSGLNMKRKGFLKLLRMILNNEVSRVVVAYPDRLVRFGFEILEETCKAHGCEIVVLNQEDKEEELVEDLVSILVSFSGKLYGMRSHKYEKVKKCAEELKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U91</accession>
    <name>Q97U91_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO3129</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90496">
        <property type="entry name" value="G90496"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989954.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U91"/>
    <dbReference type="FunCoup" id="Q97U91">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3129"/>
    <dbReference type="PaxDb" id="273057-SSO3129"/>
    <dbReference type="EnsemblBacteria" id="AAK43230">
        <property type="protein sequence ID" value="AAK43230"/>
        <property type="gene ID" value="SSO3129"/>
    </dbReference>
    <dbReference type="GeneID" id="1453149"/>
    <dbReference type="KEGG" id="sso:SSO3129"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3235"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_34_0_2"/>
    <dbReference type="InParanoid" id="Q97U91"/>
    <dbReference type="PhylomeDB" id="Q97U91"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17504">
        <property type="entry name" value="MFS_MMR_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000928">
        <property type="entry name" value="Major facilitator superfamily MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000038">
        <property type="entry name" value="Major facilitator superfamily MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF192">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="409"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="451"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="13"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="487" mass="53392" checksum="3BBF5CA0B4EC3D4A" modified="2001-10-01" version="1">MSDNYDLKYAYKALFILAPIAIAVMYTEAMLIPSLPTIANDFNVNSATVSWILTAYLVSGVISNPIVGKLGDIYGKKRILVYVMIIYTIAVTFNGFAPNFTLFIIFRTIQGIGLGMFPLAFSLIREEFPPHLVPRAQGIVSAMFGIGSAISLPIAATVAQDLGWQYNYHFIIPFVILLTYLTSREIRESKYTNPNTKIDYIGALILGLSLALMTIALSEAPTWGWLSLNFLTTVLIGIFLFFGFILYEIRAPFPLISINLLREKNVLAANIAAFVAGFGIFMAYQAITYLLELPNPVGFNLDILTTGLLMWPVSLMQMIGALLSSRLILRTGTKFIVVISSIILSFFYFILGLLAIGGSSNNLATIITLSSLATLGAAMLNVVLINILTFSVERRVLGIATGMNTVFRLIGGAFGPSIAGSLLSTYYQYLVYPMILNGQETFLPVQLPSDYAFQLTFFIASVAGLMMVIIGSMTRDIKIRGTIVSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UB8</accession>
    <name>Q97UB8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90493">
        <property type="entry name" value="D90493"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UB8"/>
    <dbReference type="STRING" id="273057.SSO3099"/>
    <dbReference type="PaxDb" id="273057-SSO3099"/>
    <dbReference type="EnsemblBacteria" id="AAK43203">
        <property type="protein sequence ID" value="AAK43203"/>
        <property type="gene ID" value="SSO3099"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3099"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3205"/>
    <dbReference type="eggNOG" id="arCOG06001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_544713_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UB8"/>
    <dbReference type="PhylomeDB" id="Q97UB8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="548" mass="58758" checksum="0A92B20CE888FDEF" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UC5</accession>
    <name>Q97UC5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90492">
        <property type="entry name" value="E90492"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UC5"/>
    <dbReference type="STRING" id="273057.SSO3090"/>
    <dbReference type="PaxDb" id="273057-SSO3090"/>
    <dbReference type="EnsemblBacteria" id="AAK43196">
        <property type="protein sequence ID" value="AAK43196"/>
        <property type="gene ID" value="SSO3090"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3090"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3198"/>
    <dbReference type="eggNOG" id="arCOG07341">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_910926_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UC5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="36584" checksum="64BC682C6D432B46" modified="2001-10-01" version="1">MITFCYLQLLGFTVRGIGMKVLHPYFYTFLFFNFTVFLPILINGIRIVSLWYSPLSDTGQQTFILPFLIFTVIYLACSSIFGYFLYRHKENKFILVKGDFTVLNIMKSLLNDKAFRKILIFYSVSYLVSFLIVSGVILVPNINISPYFLKLTTITYDAYGIQIYFNFFVLNIPMIIFALLNTLILSFAFMLSYYTISLIYVSYNLMEWKVPKTFRITATNIAGGFLTASVPSIGTIAGICCLTPTAINSLLYLASSSFPLISKNLVWKYTIYMGMPLLSTISQLILLSSPVFAGVVILSLSLYSLIQISKKLREGLLWQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UD6</accession>
    <name>Q97UD6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99491">
        <property type="entry name" value="B99491"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989099.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UD6"/>
    <dbReference type="STRING" id="273057.SSO3081"/>
    <dbReference type="PaxDb" id="273057-SSO3081"/>
    <dbReference type="EnsemblBacteria" id="AAK43185">
        <property type="protein sequence ID" value="AAK43185"/>
        <property type="gene ID" value="SSO3081"/>
    </dbReference>
    <dbReference type="GeneID" id="84059605"/>
    <dbReference type="KEGG" id="sso:SSO3081"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3187"/>
    <dbReference type="eggNOG" id="arCOG08498">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2009980_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UD6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="128" mass="14471" checksum="EA40FC0574E5B8C3" modified="2001-10-01" version="1">MMRVGIYLISLGIFILVLGEITFPLNTTLNVNNSSMYIIPPWYEKAKVSVNSGSFLIVYHNYTYILLVKGSITIKPASILYGVGNASILLEGYKIFYNQSYIAVGIFLIIVGVGLEIIRFKRRKDHQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97US9</accession>
    <name>Q97US9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C4-dicarboxylate transporter/malic acid transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2915</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90470">
        <property type="entry name" value="H90470"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97US9"/>
    <dbReference type="PaxDb" id="273057-SSO2915"/>
    <dbReference type="DNASU" id="1452942"/>
    <dbReference type="EnsemblBacteria" id="AAK43023">
        <property type="protein sequence ID" value="AAK43023"/>
        <property type="gene ID" value="SSO2915"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2915"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3005"/>
    <dbReference type="eggNOG" id="arCOG08507">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_958530_0_0_2"/>
    <dbReference type="InParanoid" id="Q97US9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="entry name" value="Voltage-dependent anion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="287"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="35258" checksum="0779A8DBC23D6C39" modified="2001-10-01" version="1">MYNSEAIHKAKYKIKLLLSNNMSLDDIFGQPISFTMLMGIAGISIASYYDRLTSLSLSSFGISIALLSLLTFFSILNLNRMKYEMVDIMAVISGLTLFIARLRLLYSSLFYFIPLVILSIFYLIIAYRVLRSLKQVSFKYHLLGVAITLLSIELRSYLPILSFIFVCIGIVMYLMVTSLLLKRILKEKNVIKLIDGSIWIQMGLPALISFAISPFSKVVSLVFWYLALIFLPLVILASLIKLISIEISIRQFHPSLWSVIFPQAVLSTATLNIVRVRFIALPILYEISISVLFSALSLFLLFLSIAVFSITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UV7</accession>
    <name>Q97UV7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SpoVT-AbrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO11387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90467">
        <property type="entry name" value="B90467"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924016.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UV7"/>
    <dbReference type="STRING" id="273057.SSO11387"/>
    <dbReference type="PaxDb" id="273057-SSO11387"/>
    <dbReference type="EnsemblBacteria" id="AAK42993">
        <property type="protein sequence ID" value="AAK42993"/>
        <property type="gene ID" value="SSO11387"/>
    </dbReference>
    <dbReference type="GeneID" id="1452912"/>
    <dbReference type="KEGG" id="sso:SSO11387"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2975"/>
    <dbReference type="eggNOG" id="arCOG04480">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173007_2_0_2"/>
    <dbReference type="InParanoid" id="Q97UV7"/>
    <dbReference type="PhylomeDB" id="Q97UV7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="4"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="74" mass="8909" checksum="6272929642D6DAFB" modified="2001-10-01" version="1">MEKVVIKKVDDQRRIVIPKEWRAGFDTEDLIMVLKDDRIEIYPKVSNVSKLIDSVEVDELPDDWHELKRKIYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UW6</accession>
    <name>Q97UW6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Acyl-CoA dehydrogenase (Acd-6)</fullName>
            <ecNumber evidence="12">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">acd-6</name>
        <name evidence="12" type="ordered locus">SSO2877</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-valine degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="12"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90465">
        <property type="entry name" value="G90465"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989823.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UW6"/>
    <dbReference type="FunCoup" id="Q97UW6">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2877"/>
    <dbReference type="PaxDb" id="273057-SSO2877"/>
    <dbReference type="EnsemblBacteria" id="AAK42982">
        <property type="protein sequence ID" value="AAK42982"/>
        <property type="gene ID" value="SSO2877"/>
    </dbReference>
    <dbReference type="GeneID" id="1452901"/>
    <dbReference type="KEGG" id="sso:SSO2877"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2961"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_2"/>
    <dbReference type="InParanoid" id="Q97UW6"/>
    <dbReference type="PhylomeDB" id="Q97UW6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0101">Branched-chain amino acid catabolism</keyword>
    <keyword evidence="6 8" id="KW-0274">FAD</keyword>
    <keyword evidence="5 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="11">
        <location>
            <begin position="16"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="10">
        <location>
            <begin position="131"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="9">
        <location>
            <begin position="241"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK42982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="400" mass="43963" checksum="CE3A8630A52873BE" modified="2001-10-01" version="1">MVLPFSSVEAFSINISEKHELFRRAVREFMERDVAPYVEKGEKEGTVPKEVLEKAKEIGLYGVAVPEQYGGQGGDTLMTAIAQEEISRVWASLSTRISVGGLFMTPILLFGNEEQKKKYVTPVARGDKVAAFANTEPVAGSDVAGIQTVAKKINGKYVINGRKIFITNGGIADYYVVTARTSPPEPNARWKGISMFIVEREWKGVKVLNRIETMGLRASNTAELAFEDVEVPAENLLGEEGNGFKYAMATFDRTRVGVAAQGVGVAQAALERMVTYSTQRFAFQSPLIGFQMVQEKIAESLTEVNTARLLTYWAANLFDRGLENEAIVAASMAKLYATDIAEKVAIRAITVHGGYGVATSTGVERLLRDVEVMRIYEGANDIQKLVILRETARRLLGIKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UY2</accession>
    <name>Q97UY2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2857</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90463">
        <property type="entry name" value="G90463"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989847.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UY2"/>
    <dbReference type="STRING" id="273057.SSO2857"/>
    <dbReference type="PaxDb" id="273057-SSO2857"/>
    <dbReference type="EnsemblBacteria" id="AAK42966">
        <property type="protein sequence ID" value="AAK42966"/>
        <property type="gene ID" value="SSO2857"/>
    </dbReference>
    <dbReference type="GeneID" id="1452885"/>
    <dbReference type="KEGG" id="sso:SSO2857"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2944"/>
    <dbReference type="eggNOG" id="arCOG07333">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1943951_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UY2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="138" mass="15925" checksum="6497984F8E6FCF5F" modified="2001-10-01" version="1">MINRRGLTIMTVFSFIYAILELGIQWDPSKVLSSPAWMKSVFTPTVSLYFYRVIYILIFGFPSYLASGKLLSVETVWYLIYGSIVEDIMYWIVDLKLPFSWAWFYPVHFGIPIDDLIGVVILAAMYKLIKQKSKAGMS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2019-07-03" modified="2025-06-18" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UY9</accession>
    <name>GLCU_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucose import system permease protein GlcU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">glcU</name>
        <name evidence="7" type="ordered locus">SSO2849</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="J. Bacteriol." volume="181" first="4285" last="4291">
            <title>Glucose transport in the extremely thermoacidophilic Sulfolobus solfataricus involves a high-affinity membrane-integrated binding protein.</title>
            <authorList>
                <person name="Albers S.V."/>
                <person name="Elferink M.G."/>
                <person name="Charlebois R.L."/>
                <person name="Sensen C.W."/>
                <person name="Driessen A.J."/>
                <person name="Konings W.N."/>
            </authorList>
            <dbReference type="PubMed" id="10400586"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Mol. Microbiol." volume="39" first="1494" last="1503">
            <title>Sugar transport in Sulfolobus solfataricus is mediated by two families of binding protein-dependent ABC transporters.</title>
            <authorList>
                <person name="Elferink M.G."/>
                <person name="Albers S.V."/>
                <person name="Konings W.N."/>
                <person name="Driessen A.J."/>
            </authorList>
            <dbReference type="PubMed" id="11260467"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="5 6">Part of the ABC transporter complex GlcSTUV involved in glucose uptake. Responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The complex is composed of two ATP-binding proteins (GlcV), two transmembrane proteins (GlcT and GlcU) and a solute-binding protein (GlcS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90462">
        <property type="entry name" value="H90462"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UY9"/>
    <dbReference type="SMR" id="Q97UY9"/>
    <dbReference type="FunCoup" id="Q97UY9">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2849"/>
    <dbReference type="TCDB" id="3.A.1.1.13">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2849"/>
    <dbReference type="EnsemblBacteria" id="AAK42959">
        <property type="protein sequence ID" value="AAK42959"/>
        <property type="gene ID" value="SSO2849"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2849"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2935"/>
    <dbReference type="eggNOG" id="arCOG00159">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_2_2"/>
    <dbReference type="InParanoid" id="Q97UY9"/>
    <dbReference type="PhylomeDB" id="Q97UY9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054927">
        <property type="entry name" value="GlcU_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040932">
        <property type="entry name" value="ABC_arch_GlcU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43879">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43879:SF1">
        <property type="entry name" value="GLUCOSE IMPORT SYSTEM PERMEASE PROTEIN GLCU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0762">Sugar transport</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000447614" description="Glucose import system permease protein GlcU">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="2">
        <location>
            <begin position="71"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="11260467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="10400586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6">
        <source>
            <dbReference type="PubMed" id="11260467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="287" mass="31182" checksum="85002C1A317C0315" modified="2001-10-01" version="1">MDKKMRPVIKASLHYLALAIVSVIWLIPVYAMLINGFKSNFEVLSTPVLVPPTKITFEAYVSVLLSLAKPLINSLIIVIPTSFISAFLGAMGAYFFYTLSYSFSRASSAISDVLFSLISLATFIPQEATLLPLTRLIVSMGLLDSYIGIIFALLIFYIPTGALLMSMFISVIPRSLIEAAKMDGTGDLKIFMKIVFPLSMPGFISTLIFIIIQAWNNFFIPLVLVTTPGMKLTSIAVLSYSGAYGTLYNDTFAAGMVASIIPLAIFVFLGRYFIRGLMALGGGGKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UZ4</accession>
    <name>Q97UZ4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Third ORF in transposon ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO11280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90462">
        <property type="entry name" value="B90462"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UZ4"/>
    <dbReference type="STRING" id="273057.SSO11280"/>
    <dbReference type="PaxDb" id="273057-SSO11280"/>
    <dbReference type="EnsemblBacteria" id="AAK42953">
        <property type="protein sequence ID" value="AAK42953"/>
        <property type="gene ID" value="SSO11280"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11280"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2928"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208838_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UZ4"/>
    <dbReference type="PhylomeDB" id="Q97UZ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="52" mass="6092" checksum="9DF0972AC5BE83ED" modified="2001-10-01" version="1">MIAIKVNPIIKRFYEEHKGRLRGKKLITACARKLAVITWAVLYYNKPFDANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V02</accession>
    <name>Q97V02_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter, binding protein (Branched chain amino acid)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2835</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90461">
        <property type="entry name" value="B90461"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923989.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V02"/>
    <dbReference type="FunCoup" id="Q97V02">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2835"/>
    <dbReference type="PaxDb" id="273057-SSO2835"/>
    <dbReference type="EnsemblBacteria" id="AAK42945">
        <property type="protein sequence ID" value="AAK42945"/>
        <property type="gene ID" value="SSO2835"/>
    </dbReference>
    <dbReference type="GeneID" id="1452864"/>
    <dbReference type="KEGG" id="sso:SSO2835"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2921"/>
    <dbReference type="eggNOG" id="arCOG01021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_5_1_2"/>
    <dbReference type="InParanoid" id="Q97V02"/>
    <dbReference type="PhylomeDB" id="Q97V02"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051010">
        <property type="entry name" value="BCAA_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30483:SF40">
        <property type="entry name" value="HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30483">
        <property type="entry name" value="LEUCINE-SPECIFIC-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Receptor ligand binding region" evidence="5">
        <location>
            <begin position="3"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="361" mass="39459" checksum="CBCE97405B496B10" modified="2001-10-01" version="1">MQVQVVIEDTATNPQTALSDLQTLYSQGVKLVIGPFSSGELRQIMSYAESNGIMLFSPSSTSPALATPDRINGTIFRDVPNDTYQGAVIANIMYQQGIRAIAVIWRGDTYGDGLVSVVNKTFTALGGKVMLVARYDPSTTDFSSYVSTLANTISQLINQYGKDHVAVYDVSFEEGASILQLASQYPQLTEVKWYGSDGTAGVGSIVQNPVAAQVAVETLFLNPIAAPAHTVFYDQLTQHFNKTLQAYSYITYDIFWIYFYSVLKVGSDNVTAIKSILPKVAAKFYGASGYHLFDKYGDRVSGIYQLQIVNKTASGYEWTIYGVYDVTTHTLTIGFPPAAQPPQGYLYPDDPQAMSKLSSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V03</accession>
    <name>Q97V03_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2834</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99461">
        <property type="entry name" value="A99461"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923988.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V03"/>
    <dbReference type="STRING" id="273057.SSO2834"/>
    <dbReference type="PaxDb" id="273057-SSO2834"/>
    <dbReference type="EnsemblBacteria" id="AAK42944">
        <property type="protein sequence ID" value="AAK42944"/>
        <property type="gene ID" value="SSO2834"/>
    </dbReference>
    <dbReference type="GeneID" id="1452863"/>
    <dbReference type="KEGG" id="sso:SSO2834"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2920"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V03"/>
    <dbReference type="PhylomeDB" id="Q97V03"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="80"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37371" checksum="9F415D9F3B0741A6" modified="2001-10-01" version="1">MQTIQVSKTELKSLAITLATNNINVISQDLDPEIVKAAPSLLTGNRGKYYLKVVRRGEKVISKGQRTFKFYPIYREVKGEINVVAVDETGLTVGEKEQEKAEGFLLYNWKRKGVKMRSLDLVYPLRLPLLVEVADLRSDSPSQFLLRSVREVSQYMEIDYVVADAGFLNLGVIKEMPVKTIVRGKSNLKGFKELSNVPLVEKRYEVKDKVYVAYRVLEFEGLYYYDVVYVKGKPRHFMFVTNFEGDPYELAELYRLRWQVEEGFKVRKARIRYVRKLSNKIFLFLYYTVLDSAWNLVNHLLFNFKSTCKKVLSFDSFVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V43</accession>
    <name>Q97V43_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acid phosphatase, putative (AcpA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">acpA</name>
        <name evidence="3" type="ordered locus">SSO2789</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90455">
        <property type="entry name" value="G90455"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991941.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V43"/>
    <dbReference type="SMR" id="Q97V43"/>
    <dbReference type="STRING" id="273057.SSO2789"/>
    <dbReference type="PaxDb" id="273057-SSO2789"/>
    <dbReference type="EnsemblBacteria" id="AAK42902">
        <property type="protein sequence ID" value="AAK42902"/>
        <property type="gene ID" value="SSO2789"/>
    </dbReference>
    <dbReference type="GeneID" id="1452821"/>
    <dbReference type="KEGG" id="sso:SSO2789"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2875"/>
    <dbReference type="eggNOG" id="arCOG05966">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038122_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V43"/>
    <dbReference type="PhylomeDB" id="Q97V43"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042578">
        <property type="term" value="F:phosphoric ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16013">
        <property type="entry name" value="AcpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007312">
        <property type="entry name" value="Phosphoesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31956:SF1">
        <property type="entry name" value="NON-SPECIFIC PHOSPHOLIPASE C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31956">
        <property type="entry name" value="NON-SPECIFIC PHOSPHOLIPASE C4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04185">
        <property type="entry name" value="Phosphoesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="433"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="487"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="511" mass="59014" checksum="A365376857684E68" modified="2001-10-01" version="1">MKRVLILTLLPLLLINTTFHANTTTPIRHVIIIIEENHSFDNLFGTYPFGYPPILNNITLSLMYPYGLYENYTQLQGAKNGVLYWISVPDIPWLPIAYSHPYYSNAYSTRDPYEGWDSYHGDYWFDTDKGFVYYSGPQSLAYFSYQQTWILWDYAEEYVLADDYFSPVLGLTEPNRIAYLTGFSPNFYSDDATNVIPFNYSIMYQLQLNNVSWGYFVYKLNGIPWPLDAFNQINMSHIYDLKNFFGDLKNGSLPAVSWVMLIGGESDEYDMHPPHNITAGEIMIANITNAVMESQYWNSTVIFITFDEGGGYYDQVIPPSINHYGLGQRIPLLIISPYAKEGYIDNYTLSGYTLLAFIDYNWHLPWLTKYVEDSDLQGLLQAFNFSTKPRAPIILTPSNWTYPLPLQYPIHYGYIATVPNYKGYAEIYPMPEMAFLLPLDVIAFVLLFISFKKRTLIKLTFLLFVVSLGISGYIYEINNIYQFISEYYFAASFIGFLIGGVLLARQYSRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V90</accession>
    <name>Q97V90_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2173 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2745</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90449">
        <property type="entry name" value="H90449"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990269.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V90"/>
    <dbReference type="PaxDb" id="273057-SSO2745"/>
    <dbReference type="EnsemblBacteria" id="AAK42855">
        <property type="protein sequence ID" value="AAK42855"/>
        <property type="gene ID" value="SSO2745"/>
    </dbReference>
    <dbReference type="GeneID" id="1452774"/>
    <dbReference type="KEGG" id="sso:SSO2745"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2824"/>
    <dbReference type="eggNOG" id="arCOG05211">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170985_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V90"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018685">
        <property type="entry name" value="DUF2173"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09941">
        <property type="entry name" value="DUF2173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006821">
        <property type="entry name" value="UCP006821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="12162" checksum="798A09776506CAC9" modified="2001-10-01" version="1">MSEKLDKLMQLKGAIAAGQYTPDGKLVEYKGPMSKEIAEMVAKMVAANTLMGTVEAESFTKISGMRWTPFLGWAVAAGEYAVCVMGNYGVFVKLAEADFNQIFKTLREVAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VB7</accession>
    <name>Q97VB7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2718</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90446">
        <property type="entry name" value="E90446"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988649.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VB7"/>
    <dbReference type="FunCoup" id="Q97VB7">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2718"/>
    <dbReference type="PaxDb" id="273057-SSO2718"/>
    <dbReference type="EnsemblBacteria" id="AAK42828">
        <property type="protein sequence ID" value="AAK42828"/>
        <property type="gene ID" value="SSO2718"/>
    </dbReference>
    <dbReference type="GeneID" id="1452747"/>
    <dbReference type="KEGG" id="sso:SSO2718"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2799"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_5_1_2"/>
    <dbReference type="InParanoid" id="Q97VB7"/>
    <dbReference type="PhylomeDB" id="Q97VB7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001056">
        <property type="entry name" value="Major facilitator superfamily MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001059">
        <property type="entry name" value="Major facilitator superfamily MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF399">
        <property type="entry name" value="FI19708P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="327"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="389"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="9"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="419" mass="46499" checksum="6DB6AB6B5C6DA267" modified="2001-10-01" version="1">MKGNLRWKIVLIIFALIIVDYIDRGLINTALPVLKSEFHISSFEAGIIGDGFTFGYLIMNPLVGYFLDKYGPKRVFSRFAILWGAVQAINVFAFSTFYFIVTRVLLGIGEAVGFPGVTKIVANWLRKDEKARGGTISDSGVNLGIVFGSLFMLGLFAIIPNQELAWRLGFLVSGLLAIILAIILGRLLYDLPEQHPKISKEELDYILSGREKVGVKTKLPLSYWLRSKNYWGYMQGLGAQAGIFFGLFTWLPLYLFYARHLSLSFTLEYTALIWSFGFIGELVGGYVVDKLIKRNPNLGFKVGFAVSSLAVTIGLAAATLISSPIEAVEILMVTFFFLRWSGIQWAAPSFLVSTELAGQFGGHIGFWETLWGIIVPIVFGATVEVTKAYLLGMEILIGIGLIYFIGTVIITNYRQIKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VD7</accession>
    <name>Q97VD7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2691</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90443">
        <property type="entry name" value="H90443"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988615.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VD7"/>
    <dbReference type="FunCoup" id="Q97VD7">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2691"/>
    <dbReference type="PaxDb" id="273057-SSO2691"/>
    <dbReference type="EnsemblBacteria" id="AAK42807">
        <property type="protein sequence ID" value="AAK42807"/>
        <property type="gene ID" value="SSO2691"/>
    </dbReference>
    <dbReference type="GeneID" id="1452726"/>
    <dbReference type="KEGG" id="sso:SSO2691"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2773"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_12_1_2"/>
    <dbReference type="InParanoid" id="Q97VD7"/>
    <dbReference type="PhylomeDB" id="Q97VD7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="8"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="74"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="133" mass="14882" checksum="39ADFE1B12AD9739" modified="2001-10-01" version="1">MKLKDVFNNTRPIRIARHTSLSEALERMDKQGVKFALIVDDSKGEEIIGIVTRSIILSSLGKGISQNEPVSKVMIKNVITINGEEDLIDTFMFMMKNNITHLLAINENGKIIGVVTLRDVLSAINKECERDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VH1</accession>
    <name>Q97VH1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Quinol oxidase (SoxABC), cytochrome B subunit, C-terminal part (SoxC)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">soxC</name>
        <name evidence="2" type="ordered locus">SSO10828</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90439">
        <property type="entry name" value="F90439"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988566.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VH1"/>
    <dbReference type="FunCoup" id="Q97VH1">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO10828"/>
    <dbReference type="PaxDb" id="273057-SSO10828"/>
    <dbReference type="EnsemblBacteria" id="AAK42773">
        <property type="protein sequence ID" value="AAK42773"/>
        <property type="gene ID" value="SSO10828"/>
    </dbReference>
    <dbReference type="GeneID" id="84060507"/>
    <dbReference type="KEGG" id="sso:SSO10828"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2737"/>
    <dbReference type="eggNOG" id="arCOG07257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3283025_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VH1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="40" mass="4389" checksum="37553DCFB8AF9969" modified="2001-10-01" version="1">MNKLGIAILALIFGATAFMAGFMAYNLLWDSVKIIAMLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VI7</accession>
    <name>Q97VI7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Carbon monoxide dehydrogenase, medium chain. (CutB-2)</fullName>
            <ecNumber evidence="5">1.2.7.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cutB-2</name>
        <name evidence="5" type="ordered locus">SSO2636</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.4" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90437">
        <property type="entry name" value="F90437"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988542.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VI7"/>
    <dbReference type="FunCoup" id="Q97VI7">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2636"/>
    <dbReference type="PaxDb" id="273057-SSO2636"/>
    <dbReference type="EnsemblBacteria" id="AAK42757">
        <property type="protein sequence ID" value="AAK42757"/>
        <property type="gene ID" value="SSO2636"/>
    </dbReference>
    <dbReference type="GeneID" id="1452676"/>
    <dbReference type="KEGG" id="sso:SSO2636"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2720"/>
    <dbReference type="eggNOG" id="arCOG01926">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058050_3_0_2"/>
    <dbReference type="InParanoid" id="Q97VI7"/>
    <dbReference type="PhylomeDB" id="Q97VI7"/>
    <dbReference type="BioCyc" id="MetaCyc:MONOMER-18072"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:anaerobic carbon-monoxide dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000017">
        <property type="entry name" value="Xanthine dehydrogenase, FAD binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036683">
        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051312">
        <property type="entry name" value="Diverse_Substr_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053586">
        <property type="entry name" value="Glyceraldehyde_DH_medium"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041019">
        <property type="entry name" value="glyceraldDH_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42659">
        <property type="entry name" value="XANTHINE DEHYDROGENASE SUBUNIT C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42659:SF2">
        <property type="entry name" value="XANTHINE DEHYDROGENASE SUBUNIT C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03450">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00941">
        <property type="entry name" value="FAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01092">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55447">
        <property type="entry name" value="CO dehydrogenase flavoprotein C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="282" mass="31052" checksum="C476A97CE2785412" modified="2001-10-01" version="1">MYPPDFTYVRVSSSEEATKFLESHDDARPLAGGQSLIPMLKLRVISPNYIVDLNPITSLSYVRSSFNSTKIGALTRYNEILKNDLVRVNVPLLHQAVRVVGDMQVRNLGTIGGSAANADPSADIPTVLTALNAEIILSSASGNRSVNALDFFKGAFATDLRKGEIISEIVLPNLEGYRTIYKKVVRRAGDFALVSLALAIKLRQNEIEDIRLAYGGVGERPFRALEVEKSVMGKRLNDELVEEIVSKVSSQVNPPSDTRGSSWYRREVMKVITRKALKEVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VQ2</accession>
    <name>Q97VQ2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90429">
        <property type="entry name" value="C90429"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992735.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VQ2"/>
    <dbReference type="PaxDb" id="273057-SSO2561"/>
    <dbReference type="EnsemblBacteria" id="AAK42690">
        <property type="protein sequence ID" value="AAK42690"/>
        <property type="gene ID" value="SSO2561"/>
    </dbReference>
    <dbReference type="GeneID" id="1454017"/>
    <dbReference type="KEGG" id="sso:SSO2561"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2643"/>
    <dbReference type="eggNOG" id="arCOG03853">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1500345_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VQ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="182" mass="21278" checksum="77B774FB54F1E1B7" modified="2001-10-01" version="1">MKNFTLSRAVFLLPFLLWWVPIIGPFFTGLLLSYRFKTNYRLSIVSSVAYSVFLSVLTTYIFYIVRITILGYPFYYLVLVFNLIGSITCIATAYMSSRHGTFARMMRNTIELEFTVNSMNEIDEILSQYLDVSLCSKPNIRFISEDQIEITRSCNNLQINYEVIKMGRALRVKAKFENLRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VQ9</accession>
    <name>Q97VQ9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glutamine synthetase (Glutamate ammonia ligase) (GS). (GlnA-3)</fullName>
            <ecNumber evidence="7">6.3.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">glnA-3</name>
        <name evidence="7" type="ordered locus">SSO2554</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90428">
        <property type="entry name" value="D90428"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923920.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VQ9"/>
    <dbReference type="FunCoup" id="Q97VQ9">
        <property type="interactions" value="175"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2554"/>
    <dbReference type="PaxDb" id="273057-SSO2554"/>
    <dbReference type="EnsemblBacteria" id="AAK42683">
        <property type="protein sequence ID" value="AAK42683"/>
        <property type="gene ID" value="SSO2554"/>
    </dbReference>
    <dbReference type="GeneID" id="1454010"/>
    <dbReference type="KEGG" id="sso:SSO2554"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2636"/>
    <dbReference type="eggNOG" id="arCOG01909">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_1_3_2"/>
    <dbReference type="InParanoid" id="Q97VQ9"/>
    <dbReference type="PhylomeDB" id="Q97VQ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000020">
        <property type="entry name" value="Putative glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785">
        <property type="entry name" value="GAMMA-GLUTAMYLPUTRESCINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785:SF12">
        <property type="entry name" value="TYPE-1 GLUTAMINE SYNTHETASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS catalytic" evidence="6">
        <location>
            <begin position="105"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="429" mass="49237" checksum="3EDF913A749984DF" modified="2001-10-01" version="1">MNVEKELREKGVEILRFTWVGLDGYIRSKGAYIDHVDELLKTGIGLTMAMMSFTPMDYISPYGTFGPQDEDVFLTPDLNTLSVFPPSAIVICNLYKHGNPWNYDPRSILIKTIEKAKEQYDFDFKSAFEIEFYLTKDKKPLDDARCFDPSAFYNNQIIPEIAKTLRQINIEPIRIIKEYGPGQYEFDIMHKDALRSADEVVIFKEVARQVASKHGVEANFMPKPFNKLAGSGLHLNISLWKDNQNLFYSHNDKYGLSDLAYNFIAGLLEHAKALTAIASPTINSYKRLVPGSWAPTKITYGYNNKSAMIRIPTPYPGMSQLDRRIEYRVPDPSTNPYLLLTAVIEAGLDGIERGLKPPEAVNENAYYRKDIDDIPRNLREALNELRKDTKLVERIGKEIIGEFIKVKLAEVEEYESLVTDWEYEVYRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VT4</accession>
    <name>Q97VT4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycerol-3-phosphate dehydrogenase chain A (GlpA)</fullName>
            <ecNumber evidence="7">1.1.5.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">glpA</name>
        <name evidence="7" type="ordered locus">SSO2526</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.5.3" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90425">
        <property type="entry name" value="A90425"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VT4"/>
    <dbReference type="FunCoup" id="Q97VT4">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2526"/>
    <dbReference type="PaxDb" id="273057-SSO2526"/>
    <dbReference type="EnsemblBacteria" id="AAK42656">
        <property type="protein sequence ID" value="AAK42656"/>
        <property type="gene ID" value="SSO2526"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2526"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2602"/>
    <dbReference type="eggNOG" id="arCOG00753">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015740_0_1_2"/>
    <dbReference type="InParanoid" id="Q97VT4"/>
    <dbReference type="PhylomeDB" id="Q97VT4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004368">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006127">
        <property type="term" value="P:glycerol-3-phosphate shuttle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000447">
        <property type="entry name" value="G3P_DH_FAD-dep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985:SF15">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01001">
        <property type="entry name" value="FADG3PDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="6">
        <location>
            <begin position="20"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="445" mass="48644" checksum="F880333FA7F6D3ED" modified="2001-10-01" version="1">MSTFLMLNPLHIIMEIKSTVTVIGGGVSGLFTALDLALRGIDVTLVDRGDIGYGTSGKFHGLLHSGARYAVTDPDSARECIQENKIISKIAPHTVKDTGGVFLGITDDDLQFSETFIKALNKVGIESKVIDVKEVLQKEPFINRDTKMAIWVPDKVVYGYDLLASVAITASLNGAKILTYNEVVEIIRENNNVKGVKVLDKINNNTNVIKSDIIVNTAGPWSFNIIKMAGLEEIPIMPTAGIIVVFDKRVNNMVINRLRPPSDGDIIVPYADSSILGTTATIIEDPDNFTISDEDIAMLVNEGAYLIPKLKNMRVVRSYASVRPLIKSEVSAREASRDFRIIDHEKENGLSGLVSVIGGKFTTGRLAGERVADLVSSKLGIKSASKTATTKLLSPNDINLLNYAEKIRLPKVIVRSILDRKGSLDEERYLSSLYILLSLISRGTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VV6</accession>
    <name>Q97VV6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-oxoacyl-(Acyl carrier protein) reductase (FabG-7)</fullName>
            <ecNumber evidence="3">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fabG-7</name>
        <name evidence="3" type="ordered locus">SSO2500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90422">
        <property type="entry name" value="C90422"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923902.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VV6"/>
    <dbReference type="FunCoup" id="Q97VV6">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2500"/>
    <dbReference type="PaxDb" id="273057-SSO2500"/>
    <dbReference type="EnsemblBacteria" id="AAK42634">
        <property type="protein sequence ID" value="AAK42634"/>
        <property type="gene ID" value="SSO2500"/>
    </dbReference>
    <dbReference type="GeneID" id="15296772"/>
    <dbReference type="KEGG" id="sso:SSO2500"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2579"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_2"/>
    <dbReference type="InParanoid" id="Q97VV6"/>
    <dbReference type="PhylomeDB" id="Q97VV6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004746">
        <property type="entry name" value="PRK06077.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43943">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE (SDR FAMILY) MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43943:SF2">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="252" mass="26848" checksum="B2557241D106DC0C" modified="2001-10-01" version="1">MYSLKDKVVVVTGSGRGIGRAIAVRLAKEGSLVVVNAKKRADEMNETIKIIKENGGEAIGILADVSTREGCETLAKATIDRYRVADILINNAGLGLFSPFLNVDDKLLDKHISTDFKSVVYCSQSLAKEMRDGGAIVNIASVAGVSPAYGLSIYGAMKAAVIALTKYLALELAPKIRVNAIAPGFVKTKLGESMFQVLGMSEKEFGEKFTLMGKILDPEEVAEFTAAILKIESLTGQVFVLDSGESLKGGIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-05-10" modified="2025-06-18" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VW7</accession>
    <name>OGT_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methylated-DNA--protein-cysteine methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.63</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">6-O-methylguanine-DNA methyltransferase</fullName>
            <shortName evidence="1">MGMT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">O-6-methylguanine-DNA-alkyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ogt</name>
        <name type="ordered locus">SSO2487</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = S-methyl-L-cysteinyl-[protein] + a 2'-deoxyguanosine in DNA</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text>This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MGMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90420">
        <property type="entry name" value="H90420"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923891.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="4ZYD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.68 A"/>
        <property type="chains" value="A=1-151"/>
    </dbReference>
    <dbReference type="PDB" id="4ZYE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.85 A"/>
        <property type="chains" value="A=1-151"/>
    </dbReference>
    <dbReference type="PDB" id="4ZYG">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B=1-151"/>
    </dbReference>
    <dbReference type="PDB" id="4ZYH">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A=1-151"/>
    </dbReference>
    <dbReference type="PDB" id="5LLQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B=1-151"/>
    </dbReference>
    <dbReference type="PDB" id="6GA0">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-151"/>
    </dbReference>
    <dbReference type="PDB" id="8AES">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J=1-151"/>
    </dbReference>
    <dbReference type="PDBsum" id="4ZYD"/>
    <dbReference type="PDBsum" id="4ZYE"/>
    <dbReference type="PDBsum" id="4ZYG"/>
    <dbReference type="PDBsum" id="4ZYH"/>
    <dbReference type="PDBsum" id="5LLQ"/>
    <dbReference type="PDBsum" id="6GA0"/>
    <dbReference type="PDBsum" id="8AES"/>
    <dbReference type="AlphaFoldDB" id="Q97VW7"/>
    <dbReference type="SMR" id="Q97VW7"/>
    <dbReference type="FunCoup" id="Q97VW7">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2487"/>
    <dbReference type="PaxDb" id="273057-SSO2487"/>
    <dbReference type="EnsemblBacteria" id="AAK42623">
        <property type="protein sequence ID" value="AAK42623"/>
        <property type="gene ID" value="SSO2487"/>
    </dbReference>
    <dbReference type="GeneID" id="84060749"/>
    <dbReference type="KEGG" id="sso:SSO2487"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2568"/>
    <dbReference type="eggNOG" id="arCOG02724">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_52_2_2"/>
    <dbReference type="InParanoid" id="Q97VW7"/>
    <dbReference type="PhylomeDB" id="Q97VW7"/>
    <dbReference type="BRENDA" id="2.1.1.63">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97VW7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000214">
        <property type="entry name" value="Methylated-DNA--protein-cysteine methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.70">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00772">
        <property type="entry name" value="OGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008332">
        <property type="entry name" value="MethylG_MeTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023546">
        <property type="entry name" value="MGMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036631">
        <property type="entry name" value="MGMT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10815">
        <property type="entry name" value="METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10815:SF13">
        <property type="entry name" value="METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02870">
        <property type="entry name" value="Methyltransf_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53155">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000139387" description="Methylated-DNA--protein-cysteine methyltransferase">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; methyl group acceptor" evidence="1">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="3"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="12"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="23"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="33"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="39"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="90"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="101"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="112"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="119"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="125"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00772"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="4ZYD"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="4ZYE"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="5LLQ"/>
        </source>
    </evidence>
    <sequence length="151" mass="17037" checksum="8457864CD5BC8F99" modified="2001-10-01" version="1">MLVYGLYKSPLGYITVAKDDKGFIMLDFCDCVEGNSRDDSSFTEFFHKLDLYFEGKPINLREPINLKTYPFRLSVFKEVMKIPWGKVMTYKQIADSLGTSPRAVGMALSKNPILLIIPCHRVIAENGIGGYSRGVKLKRALLELEGVKIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W01</accession>
    <name>Q97W01_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribokinase (RbsK-2)</fullName>
            <ecNumber evidence="5">2.7.1.15</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rbsK-2</name>
        <name evidence="5" type="ordered locus">SSO2449</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.15" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90416">
        <property type="entry name" value="F90416"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993139.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W01"/>
    <dbReference type="FunCoup" id="Q97W01">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2449"/>
    <dbReference type="PaxDb" id="273057-SSO2449"/>
    <dbReference type="EnsemblBacteria" id="AAK42589">
        <property type="protein sequence ID" value="AAK42589"/>
        <property type="gene ID" value="SSO2449"/>
    </dbReference>
    <dbReference type="GeneID" id="1453916"/>
    <dbReference type="KEGG" id="sso:SSO2449"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2527"/>
    <dbReference type="eggNOG" id="arCOG00014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_2_2_2"/>
    <dbReference type="InParanoid" id="Q97W01"/>
    <dbReference type="PhylomeDB" id="Q97W01"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019206">
        <property type="term" value="F:nucleoside kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004747">
        <property type="term" value="F:ribokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01942">
        <property type="entry name" value="ribokinase_group_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000115">
        <property type="entry name" value="PfkB domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584:SF166">
        <property type="entry name" value="RIBOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="4">
        <location>
            <begin position="5"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="283" mass="31166" checksum="DD0F05088DD0B02C" modified="2001-10-01" version="1">MIHLAVGRFNIDIIVKLDSIPPIDSSHMTDVLEIMPGGAATNYAVAVTKLGHSAKLLAKVGKSEVVRSLMEKVVELGVGLEYVEELNEKPSATLIFLRNDGTLSMVRRLGASILLTREDVKRRFGLFDVIHFASVSPNVVVRDPYAKLVSYDPGPQAKNIESVDVDILYVNEKEYEMIEDKNIRARFIVIKMGKKGAKIITETEECSVEPIQVEKIVDTTGAGDTFDAAFNVTYSEDKDIVKSLQVASVASGLKVSRIGGISSPTLEEVREYLRKKKPNVICK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W21</accession>
    <name>Q97W21_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methylmalonyl-CoA mutase, alpha-subunit, chain A (McmA1)</fullName>
            <ecNumber evidence="4">5.4.99.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mcmA1</name>
        <name evidence="4" type="ordered locus">SSO2425</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.4.99.2" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90414">
        <property type="entry name" value="B90414"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W21"/>
    <dbReference type="FunCoup" id="Q97W21">
        <property type="interactions" value="251"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2425"/>
    <dbReference type="PaxDb" id="273057-SSO2425"/>
    <dbReference type="EnsemblBacteria" id="AAK42569">
        <property type="protein sequence ID" value="AAK42569"/>
        <property type="gene ID" value="SSO2425"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2425"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2503"/>
    <dbReference type="eggNOG" id="arCOG04232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009523_5_1_2"/>
    <dbReference type="InParanoid" id="Q97W21"/>
    <dbReference type="PhylomeDB" id="Q97W21"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004494">
        <property type="term" value="F:methylmalonyl-CoA mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03680">
        <property type="entry name" value="MM_CoA_mutase_ICM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.240">
        <property type="entry name" value="Methylmalonyl-CoA mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006099">
        <property type="entry name" value="MeMalonylCoA_mutase_a/b_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006098">
        <property type="entry name" value="MMCoA_mutase_a_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00641">
        <property type="entry name" value="acid_CoA_mut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101:SF1">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01642">
        <property type="entry name" value="MM_CoA_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="Cobalamin (vitamin B12)-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methylmalonyl-CoA mutase alpha/beta chain catalytic" evidence="3">
        <location>
            <begin position="38"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="490"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="561" mass="64122" checksum="F82B72FC5CC23C79" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W23</accession>
    <name>Q97W23_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Type I phosphodiesterase/nucleotide pyrophosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2423</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90413">
        <property type="entry name" value="H90413"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W23"/>
    <dbReference type="STRING" id="273057.SSO2423"/>
    <dbReference type="PaxDb" id="273057-SSO2423"/>
    <dbReference type="EnsemblBacteria" id="AAK42567">
        <property type="protein sequence ID" value="AAK42567"/>
        <property type="gene ID" value="SSO2423"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2423"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2501"/>
    <dbReference type="eggNOG" id="arCOG01378">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039939_1_0_2"/>
    <dbReference type="InParanoid" id="Q97W23"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF120">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="44162" checksum="FD4EF1E0128A0F59" modified="2001-10-01" version="1">MANTVIDNIDINLKINTYNLIANMDLILPDYSGENIYTLSCFVAEYLGVKRQCLNKMNLNVNNRLVLALFDGLGWNVFSQAGVNLKAKKITTVFPSTTSTVLTTLFTALTPAEHSVLGYNTFSKRLGGVVNTLRYTYPTVSDRDSIKDSVPFSSSFPHVKSYLKEVQDKKTISIVPKGIENTEFSNATHGASGESKTYVNFWDAFYQLSQVLQQDTYDFIYFYIPDVDTLSHRYGPYAEPTIKSARDILTSILEISQKHKKYSFLITADHGHVPVTETIIFNNDQELLNMLDVPPYGDSRAIFLRTRYDVSTYLNRKYVSLKIFGKSDFERLLGKINDITALPDYIAVPTDYRAFIFNFKDKDEYDKLKGHHGGLLQEEFEIPLVILNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W84</accession>
    <name>Q97W84_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5751 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90406">
        <property type="entry name" value="A90406"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989501.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W84"/>
    <dbReference type="STRING" id="273057.SSO2352"/>
    <dbReference type="PaxDb" id="273057-SSO2352"/>
    <dbReference type="EnsemblBacteria" id="AAK42504">
        <property type="protein sequence ID" value="AAK42504"/>
        <property type="gene ID" value="SSO2352"/>
    </dbReference>
    <dbReference type="GeneID" id="1453831"/>
    <dbReference type="KEGG" id="sso:SSO2352"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2437"/>
    <dbReference type="eggNOG" id="arCOG05958">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987615_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W84"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043963">
        <property type="entry name" value="DUF5751"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19025">
        <property type="entry name" value="DUF5751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5751" evidence="1">
        <location>
            <begin position="10"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="127" mass="14884" checksum="3AA6034124D32EAA" modified="2001-10-01" version="1">MQLENKPIIVISSTNSEEIPNFVRAMFKDCRLNGSKKLVINFVSSISYPEFVQNAREALLDNIDLGVYIYIWKPEEVDQMMRRIMENHKDMKGMIIYCDDNNKHMIEKILPKIPNSIKANIIKDYCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WE9</accession>
    <name>Q97WE9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease GajA/Old nuclease/RecF-like AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90397">
        <property type="entry name" value="H90397"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991842.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WE9"/>
    <dbReference type="STRING" id="273057.SSO2277"/>
    <dbReference type="PaxDb" id="273057-SSO2277"/>
    <dbReference type="EnsemblBacteria" id="AAK42439">
        <property type="protein sequence ID" value="AAK42439"/>
        <property type="gene ID" value="SSO2277"/>
    </dbReference>
    <dbReference type="GeneID" id="1453766"/>
    <dbReference type="KEGG" id="sso:SSO2277"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2369"/>
    <dbReference type="eggNOG" id="arCOG03239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_549365_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WE9"/>
    <dbReference type="PhylomeDB" id="Q97WE9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00267">
        <property type="entry name" value="ABC_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041685">
        <property type="entry name" value="AAA_GajA/Old/RecF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051396">
        <property type="entry name" value="Bact_Antivir_Def_Nuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43581">
        <property type="entry name" value="ATP/GTP PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43581:SF4">
        <property type="entry name" value="ATP_GTP PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13175">
        <property type="entry name" value="AAA_15"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease GajA/Old nuclease/RecF-like AAA" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease GajA/Old nuclease/RecF-like AAA" evidence="1">
        <location>
            <begin position="154"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="495" mass="57049" checksum="B7B4F37972DC32A5" modified="2001-10-01" version="1">MRITEFYVSNFRSLSEVNLKDLGGFNIVVGYNGYGKTNLLSSIFLFVKNLSAGIEKRSIEDRNQEFILLWQGYDVSKPIMIGGKVEFSPEESNKIVGKNQKITLEIINKMRYNNKFVEWNLDTLYINGSPPGEDDLKVAKKLAEYASQAIEYVPIFDQTYFDETLKRMTEMNKSPINLRKYWYDFVNLVAATIPEVKGMEFWDGRRLVLNVYNLPIYIDLAASGFQRVILMLFIIWLSGNKILLIEEPEVNMHPTLQAKIMKLIKNWTDNNILQSFLTTHSPYIVEMAADSYVVMRRVNGNSTAITVKPTPDLRGTISLLNASLSNILFSRIIILTSELAEPSVIVNWLKRLNINVEDNGISIYKVSSDLELQHWLKLKNMLNLDVIFLGLCDKLDMSLKDYCVPLSREVENFYSKQALLEVLRRLGIYPDEKEIRDLGKEENLRWIINVLRKRGIEYEKLRMSIGELITDVDSIEIPKEIEILANKIKSLQATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WF5</accession>
    <name>Q97WF5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2269</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90397">
        <property type="entry name" value="A90397"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WF5"/>
    <dbReference type="PaxDb" id="273057-SSO2269"/>
    <dbReference type="EnsemblBacteria" id="AAK42432">
        <property type="protein sequence ID" value="AAK42432"/>
        <property type="gene ID" value="SSO2269"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2269"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2361"/>
    <dbReference type="eggNOG" id="arCOG05912">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1665578_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WF5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="173" mass="20179" checksum="E3FBF54DDB941F21" modified="2001-10-01" version="1">MMRNKVIIYYIPISLMKNSEYYIWVLVGSIILTIILLSMAPLVPIDEPLVYRASSGVTYNLTIPVGVSFSAFIALIIFIISILLISTFKNDYYNMIIDTSAISFVFLNYLNYYLIWYVWRPQMQMLPFLIEIVYNHAATLQLDIGQIVIVIFLYRLYKRLRKPRSLSGPDEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WM1</accession>
    <name>Q97WM1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NfeD-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2193</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90388">
        <property type="entry name" value="F90388"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992101.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WM1"/>
    <dbReference type="STRING" id="273057.SSO2193"/>
    <dbReference type="PaxDb" id="273057-SSO2193"/>
    <dbReference type="EnsemblBacteria" id="AAK42365">
        <property type="protein sequence ID" value="AAK42365"/>
        <property type="gene ID" value="SSO2193"/>
    </dbReference>
    <dbReference type="GeneID" id="1453692"/>
    <dbReference type="KEGG" id="sso:SSO2193"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2289"/>
    <dbReference type="eggNOG" id="arCOG01912">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2044513_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WM1"/>
    <dbReference type="PhylomeDB" id="Q97WM1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052165">
        <property type="entry name" value="Membrane_assoc_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002810">
        <property type="entry name" value="NfeD-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33507">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YBBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33507:SF4">
        <property type="entry name" value="NODULATION COMPETITIVENESS PROTEIN NFED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01957">
        <property type="entry name" value="NfeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141322">
        <property type="entry name" value="NfeD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="NfeD-like C-terminal" evidence="6">
        <location>
            <begin position="60"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="124" mass="13856" checksum="67161CBA45D861F3" modified="2001-10-01" version="1">MAHPVAITLIIIAILIAILVITGYISDPIIDIPTLALLGFLTYRVINVVIKTRGRNLYSYEGKIGKAVDDIKSGQEGYVIVEGEYWRALALEDIKKNEDIVVVKREGMKLMVRKKVSDIETSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WP9</accession>
    <name>Q97WP9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="2">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">bcp-1</name>
        <name evidence="13" type="ordered locus">SSO2071</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2009" name="Proteins" volume="76" first="995" last="1006">
            <title>Insights into the catalytic mechanism of the Bcp family: functional and structural analysis of Bcp1 from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="D'Ambrosio K."/>
                <person name="Limauro D."/>
                <person name="Pedone E."/>
                <person name="Galdi I."/>
                <person name="Pedone C."/>
                <person name="Bartolucci S."/>
                <person name="De Simone G."/>
            </authorList>
            <dbReference type="PubMed" id="19338062"/>
            <dbReference type="DOI" id="10.1002/prot.22408"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS)</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peroxiredoxin family. BCP/PrxQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90374">
        <property type="entry name" value="F90374"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990189.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3DRN">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.15 A"/>
        <property type="chains" value="A/B=1-153"/>
    </dbReference>
    <dbReference type="PDBsum" id="3DRN"/>
    <dbReference type="AlphaFoldDB" id="Q97WP9"/>
    <dbReference type="SMR" id="Q97WP9"/>
    <dbReference type="FunCoup" id="Q97WP9">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2071"/>
    <dbReference type="PeroxiBase" id="4323">
        <property type="entry name" value="SsolBCP"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2071"/>
    <dbReference type="EnsemblBacteria" id="AAK42253">
        <property type="protein sequence ID" value="AAK42253"/>
        <property type="gene ID" value="SSO2071"/>
    </dbReference>
    <dbReference type="GeneID" id="1453580"/>
    <dbReference type="KEGG" id="sso:SSO2071"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2148"/>
    <dbReference type="eggNOG" id="arCOG00310">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_14_2_2"/>
    <dbReference type="InParanoid" id="Q97WP9"/>
    <dbReference type="PhylomeDB" id="Q97WP9"/>
    <dbReference type="BRENDA" id="1.11.1.24">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97WP9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03017">
        <property type="entry name" value="PRX_BCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000007">
        <property type="entry name" value="Thioredoxin-dependent thiol peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050924">
        <property type="entry name" value="Peroxiredoxin_BCP/PrxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801:SF4">
        <property type="entry name" value="AHPC_TSA FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="15" id="KW-0002">3D-structure</keyword>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="12">
        <location>
            <begin position="2"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="11">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAK42253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="15">
        <source>
            <dbReference type="PDB" id="3DRN"/>
        </source>
    </evidence>
    <sequence length="153" mass="17460" checksum="5A83F52BAEA7A8E7" modified="2001-10-01" version="1">MVKVGDKAPLFEGIADNGEKISLSDYIGKHNIVLYFYPKDDTPGCTREACAFRDNWDLLKDYDVVVIGVSSDDINSHKRFKEKYKLPFILVSDPDKKIRELYGAKGFILPARITFVIDKKGIIRHIYNSQMNPANHVNEALKALKQIKEEEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WS7</accession>
    <name>Q97WS7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Metabolite transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2039</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90371">
        <property type="entry name" value="B90371"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923745.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WS7"/>
    <dbReference type="FunCoup" id="Q97WS7">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2039"/>
    <dbReference type="PaxDb" id="273057-SSO2039"/>
    <dbReference type="EnsemblBacteria" id="AAK42225">
        <property type="protein sequence ID" value="AAK42225"/>
        <property type="gene ID" value="SSO2039"/>
    </dbReference>
    <dbReference type="GeneID" id="1453552"/>
    <dbReference type="KEGG" id="sso:SSO2039"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2120"/>
    <dbReference type="eggNOG" id="arCOG02691">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_5_2"/>
    <dbReference type="InParanoid" id="Q97WS7"/>
    <dbReference type="PhylomeDB" id="Q97WS7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045:SF1">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="7"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="427" mass="46092" checksum="25385082FEC9F848" modified="2001-10-01" version="1">MKEETKVSIAAMIGIAFEFYDFLIFGFVAGILANLFFPSGNKIVSLLDTFAVFATGFAGRPLGAIVFGHLGDKIGRKYTLIITMTLMGLSSLFTGLLPGYAILGVLAPTLLTILRLLQGFSLGGEFGGGITLSAEFAEPGKRAFYTGIAQMAQGIGPLMATGLIFVFSSIMLPSAFASIGWRILFIIGAFIAVIGVMIRLKISESPVFKKVKEKNEISHFPLVDAFRIYWKRILLGLGFIIGGTTMTYATSVFASSYLENVIGVPARLVSLALTIGYIVEAISILLFAMLADKVGRKPLMVATAVGLLILVYPYFLLISTGQFSLILLAQIMYSVIGSMSTGAYATALTELFPTKVRYTALSFDYHVGVAVFGGTTPFIASYLIYATGYKLAPVYWGIAGMILTLIAYLLYKETKGTVFEGQEVLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WY7</accession>
    <name>Q97WY7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Second ORF in transposon ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1972</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90363">
        <property type="entry name" value="E90363"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WY7"/>
    <dbReference type="STRING" id="273057.SSO1972"/>
    <dbReference type="PaxDb" id="273057-SSO1972"/>
    <dbReference type="EnsemblBacteria" id="AAK42164">
        <property type="protein sequence ID" value="AAK42164"/>
        <property type="gene ID" value="SSO1972"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1972"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2049"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036902_12_2_2"/>
    <dbReference type="InParanoid" id="Q97WY7"/>
    <dbReference type="PhylomeDB" id="Q97WY7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="2">
        <location>
            <begin position="37"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="159" mass="17948" checksum="BEFEF1071AF225A3" modified="2001-10-01" version="1">MGIVLSWLRFLLEELERLEGRKREIEKELENVVSKDSLIFTIPGIGKTLGCIILARVGDVRRFSDKKRFVAYCGLDPVVESSGKSVVSRGISKKGDAVLRRAFYLAALTAIKVNLVIKRFYEDHKGRLKGKKLITACARKLAVITWAVLYYNKPFDASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WZ8</accession>
    <name>Q97WZ8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Extradiol ring-cleavage dioxygenase class III enzyme subunit B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90362">
        <property type="entry name" value="B90362"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991748.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WZ8"/>
    <dbReference type="STRING" id="273057.SSO1962"/>
    <dbReference type="PaxDb" id="273057-SSO1962"/>
    <dbReference type="EnsemblBacteria" id="AAK42153">
        <property type="protein sequence ID" value="AAK42153"/>
        <property type="gene ID" value="SSO1962"/>
    </dbReference>
    <dbReference type="GeneID" id="7806420"/>
    <dbReference type="KEGG" id="sso:SSO1962"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2036"/>
    <dbReference type="eggNOG" id="arCOG01729">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089900_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WZ8"/>
    <dbReference type="PhylomeDB" id="Q97WZ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07952">
        <property type="entry name" value="ED_3B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004183">
        <property type="entry name" value="Xdiol_dOase_suB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02900">
        <property type="entry name" value="LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53213">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Extradiol ring-cleavage dioxygenase class III enzyme subunit B" evidence="1">
        <location>
            <begin position="35"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="257" mass="29129" checksum="37663EDD3D813CA1" modified="2001-10-01" version="1">MISFILVAPHGDEIIDPKDEKSSLLNKAMREAASLVKADKYIVITPHNIRIDDHMAVILTQYAEGKRGKIKRKYLCDRGLAYEIYERAKGSGLPVVGVNFGALEGKESKIALDWGSSIPLYFLKRRRIVLLTPARGVKREILRKFGVVIGEVLRDHRKRVGVIVSADHAHTHDPKGPYGYSEYAAVYDSTIVDIFKSERFEDLMKIDDKVISEAKPDSYWQLLIMLGILESSYNKYSVKLMEYQVPSYFGMMVALLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X06</accession>
    <name>Q97X06_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1953</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90361">
        <property type="entry name" value="B90361"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X06"/>
    <dbReference type="STRING" id="273057.SSO1953"/>
    <dbReference type="PaxDb" id="273057-SSO1953"/>
    <dbReference type="EnsemblBacteria" id="AAK42145">
        <property type="protein sequence ID" value="AAK42145"/>
        <property type="gene ID" value="SSO1953"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1953"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2027"/>
    <dbReference type="eggNOG" id="arCOG03852">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1665579_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X06"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="18764" checksum="4D3AC99B198FD45F" modified="2001-10-01" version="1">MEMDKEKVLNILRNSSNLPLDLIRRLLSDKDKDIKHEAWNYVISNVRDKDFLLELLSFHDTGTRYRAWNSVPEFVERGILTLEEVIKRKEHFLEMLKDSNKVVRALSWYVTLKPLLEMNVVSLGEVLSYSPFLCELINSEFHEVVEGVMQEFKITCKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XA0</accession>
    <name>Q97XA0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1851</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90348">
        <property type="entry name" value="C90348"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XA0"/>
    <dbReference type="STRING" id="273057.SSO1851"/>
    <dbReference type="PaxDb" id="273057-SSO1851"/>
    <dbReference type="EnsemblBacteria" id="AAK42042">
        <property type="protein sequence ID" value="AAK42042"/>
        <property type="gene ID" value="SSO1851"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1851"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1640043_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XA0"/>
    <dbReference type="PhylomeDB" id="Q97XA0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="161" mass="18291" checksum="A256A73D383E2D1C" modified="2001-10-01" version="1">MNSRTLHGNTVGELKSSARVVEGGRSVPVGEFPQGEYLVEYLGTPIKLLVIDDYKGYGRRYFFSTDLNDTAEDIITTWENRWDIEVLIRELKALGLEGGSFLTWVRNSGFVALKALSLLVVQYFKYSTGLRLGAKRLARLIKSIYREAGGIKKLFKRRRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XC5</accession>
    <name>Q97XC5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3782 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1818</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99345">
        <property type="entry name" value="A99345"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923607.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XC5"/>
    <dbReference type="STRING" id="273057.SSO1818"/>
    <dbReference type="PaxDb" id="273057-SSO1818"/>
    <dbReference type="EnsemblBacteria" id="AAK42016">
        <property type="protein sequence ID" value="AAK42016"/>
        <property type="gene ID" value="SSO1818"/>
    </dbReference>
    <dbReference type="GeneID" id="1454795"/>
    <dbReference type="KEGG" id="sso:SSO1818"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1879"/>
    <dbReference type="eggNOG" id="arCOG01424">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_965117_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XC5"/>
    <dbReference type="PhylomeDB" id="Q97XC5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1010">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34314">
        <property type="entry name" value="CRENARCHAEAL PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34314:SF6">
        <property type="entry name" value="DUF3782 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="36"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="38738" checksum="4BB952C5B982A7B0" modified="2001-10-01" version="1">MSEEIINEIISNPRLLDKITSVIYEKMKNDIILQRLTKLEETVATLTRTVQENNQQIVLIWKKLDEMENEMRKRDSILEEHSKRIEELTKRVEELAKRVEEHSKILEEHSKRIEELSKRIEEHSKILEEHSKRIEELTKRVEELAKRVEEHSKILEEHSKRIEELSKRIEEHSKILEEHSKRIEELTKAFLMLKVSFDSFTSRAGIYVQRTLMELYKEALKSHGVDPSNVKHGYIVDDIGVIEKGRRFEVDFYETNDEIRLFEIKNLGDMDGIEQLIVRRRILESKGNKKQIKMYLVCNSIPKKVLLKAKKEGIVVIAGNILGERSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XG0</accession>
    <name>Q97XG0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sugar phosphate nucleotydyl transferase</fullName>
            <ecNumber evidence="2">2.7.7.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1782</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.-" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90339">
        <property type="entry name" value="G90339"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990173.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XG0"/>
    <dbReference type="FunCoup" id="Q97XG0">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1782"/>
    <dbReference type="PaxDb" id="273057-SSO1782"/>
    <dbReference type="EnsemblBacteria" id="AAK41974">
        <property type="protein sequence ID" value="AAK41974"/>
        <property type="gene ID" value="SSO1782"/>
    </dbReference>
    <dbReference type="GeneID" id="1454753"/>
    <dbReference type="KEGG" id="sso:SSO1782"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1832"/>
    <dbReference type="eggNOG" id="arCOG00667">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_1_2"/>
    <dbReference type="InParanoid" id="Q97XG0"/>
    <dbReference type="PhylomeDB" id="Q97XG0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04189">
        <property type="entry name" value="G1P_TT_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005908">
        <property type="entry name" value="G1P_thy_trans_l"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01208">
        <property type="entry name" value="rmlA_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42883">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE THYMIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42883:SF2">
        <property type="entry name" value="THYMIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="344" mass="38168" checksum="D862C26928000B57" modified="2001-10-01" version="1">MEAVILHGGQGTRLRPLTHTGPKQLIKVAGKPISQWVLEQIRDSGIKDILLILGDNTPTRVVDYYGDGSKFGVNITYVYQGKARGLADAVYRVKDLVSEKFLVYLGDNLVFYDLRKFLSFKGSASILLAKVDNPNRFGVAVINENKVIRLVEKPKEKISDLALVGVYGFTDEIFEVIEKLKPSWRGELEITDAIQGLINEGKEVNYEIIQGWWKDTGTPKDILEANSFLLDRYAERKIEGEVENSTIDGRVILEKGAVVKNSVIRGPAYIGKDTKIINSYIGPFTSIGDLSEISGSEIEYSVILDNVRIKGVSLMDSLIGNNSTVEKGTKWQKLIIGENSSVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XG9</accession>
    <name>Q97XG9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">First ORF in transposon ISC1491</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1772</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90338">
        <property type="entry name" value="F90338"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XG9"/>
    <dbReference type="SMR" id="Q97XG9"/>
    <dbReference type="PaxDb" id="273057-SSO1772"/>
    <dbReference type="EnsemblBacteria" id="AAK41965">
        <property type="protein sequence ID" value="AAK41965"/>
        <property type="gene ID" value="SSO1772"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1772"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1821"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098916_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XG9"/>
    <dbReference type="PhylomeDB" id="Q97XG9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="2">
        <location>
            <begin position="12"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="189"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="245" mass="28361" checksum="D5D7BC394B6949A5" modified="2001-10-01" version="1">MESEIKTRGFAIDVSRDKLTVAKAELVITQEKREVEVNEVREFKYDNEGIEELIKFLGEYKEGIMESTGPYFFYLHEELTEKGYKVTLINPLHLKEVFGKKTDKLDAQRLAKAFILGAVKGSYIPTGEIRELRELTRYRESLMRKITQVKNEIRKFLEMAGYKIEPFDKRGMALLEKLSRGEGLSKEERDELKEKLGRSLNDAEKLALKQLVDLLKSLEAMVKEVEDMIISKIPQPVVELSRELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XJ0</accession>
    <name>Q97XJ0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90335">
        <property type="entry name" value="G90335"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XJ0"/>
    <dbReference type="PaxDb" id="273057-SSO1746"/>
    <dbReference type="EnsemblBacteria" id="AAK41942">
        <property type="protein sequence ID" value="AAK41942"/>
        <property type="gene ID" value="SSO1746"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1746"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1791"/>
    <dbReference type="eggNOG" id="arCOG00713">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850709_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XJ0"/>
    <dbReference type="PhylomeDB" id="Q97XJ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09854">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052106">
        <property type="entry name" value="PINc/VapC_TA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38826">
        <property type="entry name" value="RIBONUCLEASE VAPC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38826:SF5">
        <property type="entry name" value="RIBONUCLEASE VAPC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="131" mass="15458" checksum="67D77465BEDBD38C" modified="2001-10-01" version="1">MKIMIDSNVFIYVLFSDPSYGERAKELLNKAENEDAYSSTLIISQVLAHLERRRKSEIIPVFINYIQQSGIKILETRWEDVIEAIKLLRNMNLSYNLWDDAIISAQIKREKIDIIFSNDKDFDILQVKREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-06-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XL8</accession>
    <name>Q97XL8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1491</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8725</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90331">
        <property type="entry name" value="B90331"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XL8"/>
    <dbReference type="EnsemblBacteria" id="AAK41905">
        <property type="protein sequence ID" value="AAK41905"/>
        <property type="gene ID" value="SSO8725"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8725"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1748"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180515_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XL8"/>
    <dbReference type="PhylomeDB" id="Q97XL8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="76" mass="8938" checksum="97C59FE4D56D5A22" modified="2001-10-01" version="1">MESEIKTRGFAIDVSRDKLTVAKAELVITQEKREVEVNEVREFKYDNEGIEELIKFLGEYKEGIMESTGPYFFYLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XM7</accession>
    <name>Q97XM7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Partial transposon ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1684</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90330">
        <property type="entry name" value="A90330"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XM7"/>
    <dbReference type="PaxDb" id="273057-SSO1684"/>
    <dbReference type="EnsemblBacteria" id="AAK41896">
        <property type="protein sequence ID" value="AAK41896"/>
        <property type="gene ID" value="SSO1684"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1684"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1736"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066381_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XM7"/>
    <dbReference type="PhylomeDB" id="Q97XM7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="3">
        <location>
            <begin position="193"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="260" mass="29388" checksum="D207F39E35F0566D" modified="2001-10-01" version="1">MEAPVAGIDISKDKLVVYFQGKYYEFQNNKQGFEGVKQVLPKGCKVAIESTGIYHINLAKYLGKEYDVRIINPFILKKFKDIRGKKSDKNDKKLAELVVSIGSEFTTSEAKELTSQWDFVTRSIARVKNRLRRDLILLGYRDSLSRKNLNEVLRGEDNAALSEIRFLLGELERLEARKREIEEELEDLVPKDSLIFTIPGIGRTLGCIILARVGDVKRFGDKKRFVAYCGPLVESSGKSVVSRGISKRGDAVLRSSILQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XQ5</accession>
    <name>Q97XQ5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Helicase of the snf2/rad54 family (Carboxy end), hypothetical</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2005" name="Cell" volume="121" first="363" last="373">
            <title>X-ray structures of the Sulfolobus solfataricus SWI2/SNF2 ATPase core and its complex with DNA.</title>
            <authorList>
                <person name="Durr H."/>
                <person name="Korner C."/>
                <person name="Muller M."/>
                <person name="Hickmann V."/>
                <person name="Hopfner K.P."/>
            </authorList>
            <dbReference type="PubMed" id="15882619"/>
            <dbReference type="DOI" id="10.1016/j.cell.2005.03.026"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 8-124</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90326">
        <property type="entry name" value="E90326"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923551.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1Z5Z">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B=8-124"/>
    </dbReference>
    <dbReference type="PDBsum" id="1Z5Z"/>
    <dbReference type="AlphaFoldDB" id="Q97XQ5"/>
    <dbReference type="SMR" id="Q97XQ5"/>
    <dbReference type="STRING" id="273057.SSO1655"/>
    <dbReference type="PaxDb" id="273057-SSO1655"/>
    <dbReference type="EnsemblBacteria" id="AAK41868">
        <property type="protein sequence ID" value="AAK41868"/>
        <property type="gene ID" value="SSO1655"/>
    </dbReference>
    <dbReference type="GeneID" id="1454647"/>
    <dbReference type="KEGG" id="sso:SSO1655"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1704"/>
    <dbReference type="eggNOG" id="arCOG00883">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_30_6_2"/>
    <dbReference type="InParanoid" id="Q97XQ5"/>
    <dbReference type="EvolutionaryTrace" id="Q97XQ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="5" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="1Z5Z"/>
        </source>
    </evidence>
    <sequence length="124" mass="14063" checksum="984E60EE3F272FED" modified="2001-10-01" version="1">MRTLPDDIISKFQNNPSVKFIVLSVKAGGFGINLTSANRVIHFDRWWNPAVEDQATDRVYRIGQTRNVIVHKLISVGTLEEKIDQLLAFKRSLFKDIISSGDSWITELSTEELRKVIELSVGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XQ6</accession>
    <name>Q97XQ6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transposase ISC1173</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1654</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Absolutely required for transposition of IS1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase 27 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90326">
        <property type="entry name" value="D90326"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XQ6"/>
    <dbReference type="STRING" id="273057.SSO1654"/>
    <dbReference type="PaxDb" id="273057-SSO1654"/>
    <dbReference type="EnsemblBacteria" id="AAK41867">
        <property type="protein sequence ID" value="AAK41867"/>
        <property type="gene ID" value="SSO1654"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1654"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1703"/>
    <dbReference type="eggNOG" id="arCOG02133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076276_0_1_2"/>
    <dbReference type="InParanoid" id="Q97XQ6"/>
    <dbReference type="PhylomeDB" id="Q97XQ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005063">
        <property type="entry name" value="Transposase_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051354">
        <property type="entry name" value="Transposase_27_IS1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033558">
        <property type="entry name" value="transpos_IS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293:SF1">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03400">
        <property type="entry name" value="DDE_Tnp_IS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37541" checksum="BA888994B34FCDF0" modified="2001-10-01" version="1">MRVTNMDLVTLAQLILMILRNLNLKPRKYELKDIALALAAYSLGVQITKTGIPPSTLYYYLRKVGIRRRGESRPTCPSCNSNRVVKNGSSRGKAKYKCKACGRTFYDVTSHRMDREQRERILKEYLNRMSMRAISRVEGKPLTTVYSLVRRVGIRAFTNLTILKGELKNFTAKSTVFDEFWTYFRVRHGVIREDLWIWTALADGVPFYEFGDRSYGTFRYLLGWLPRSEVNYTDYYCVYQVLDNRVASKKYTYLVESHNSWCRSHLARLARDTKAVTRSGRMAEYSLALLNVMYPHVFSKERTPLNETYLKGVQYIRENLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XV3</accession>
    <name>Q97XV3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99320">
        <property type="entry name" value="A99320"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923505.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XV3"/>
    <dbReference type="STRING" id="273057.SSO1605"/>
    <dbReference type="PaxDb" id="273057-SSO1605"/>
    <dbReference type="DNASU" id="1454595"/>
    <dbReference type="EnsemblBacteria" id="AAK41816">
        <property type="protein sequence ID" value="AAK41816"/>
        <property type="gene ID" value="SSO1605"/>
    </dbReference>
    <dbReference type="GeneID" id="1454595"/>
    <dbReference type="KEGG" id="sso:SSO1605"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1643"/>
    <dbReference type="eggNOG" id="arCOG01141">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1656964_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XV3"/>
    <dbReference type="PhylomeDB" id="Q97XV3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN VPS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="190" mass="22230" checksum="0AE30F005D76317A" modified="2001-10-01" version="1">MRKISIEWDGKILVLSDIHYPYCDIDEINKIMLSERPSLTVLLGDIIVSKSEDYRNFIDKLKIRKNIIYVKGDEDKFRGDFDLIKIKNNGKRFILLHGHQYFNENNEYSLAKVLKKMNDNIPPLLFCIFFRIMLRNFKDTIILGHSHALRFFKTINCVNAGTLSNVINLYNDRGYVVLDNGNIKLVQSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XW4</accession>
    <name>Q97XW4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl-CoA dehydrogenase (Acd-2)</fullName>
            <ecNumber evidence="3">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">acd-2</name>
        <name evidence="3" type="ordered locus">SSO1597</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.3.99.-" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90318">
        <property type="entry name" value="F90318"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XW4"/>
    <dbReference type="STRING" id="273057.SSO1597"/>
    <dbReference type="PaxDb" id="273057-SSO1597"/>
    <dbReference type="EnsemblBacteria" id="AAK41805">
        <property type="protein sequence ID" value="AAK41805"/>
        <property type="gene ID" value="SSO1597"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1597"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1630"/>
    <dbReference type="eggNOG" id="arCOG00856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_5_2"/>
    <dbReference type="InParanoid" id="Q97XW4"/>
    <dbReference type="PhylomeDB" id="Q97XW4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050237">
        <property type="entry name" value="ATP-dep_AMP-bd_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767:SF11">
        <property type="entry name" value="MEDIUM-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="1">
        <location>
            <begin position="14"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="2">
        <location>
            <begin position="427"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="516" mass="57858" checksum="56A38F5BCA096207" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XY7</accession>
    <name>Q97XY7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1568</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90315">
        <property type="entry name" value="F90315"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XY7"/>
    <dbReference type="STRING" id="273057.SSO1568"/>
    <dbReference type="PaxDb" id="273057-SSO1568"/>
    <dbReference type="EnsemblBacteria" id="AAK41781">
        <property type="protein sequence ID" value="AAK41781"/>
        <property type="gene ID" value="SSO1568"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1568"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1604"/>
    <dbReference type="eggNOG" id="arCOG06017">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121284_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XY7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="187" mass="21723" checksum="AB505A671CDD4830" modified="2001-10-01" version="1">MYMCLLIINNVTRPIISNDIVLLRDNSFSKVKDYLRVLSSDKRDGEISKSYFYFIVDRLRRMELLIDNAIGFKAILPFTVNNKGISLKEGIMYITNDKQLIYFNYYDATYQCDRCSITTFSCVPSLKKIARELDVKIRSDIPGIAWYELLEDIQHYLLEASISLRVKISEIGKSFEAIKVGEYARDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y42</accession>
    <name>Q97Y42_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">One of two inversely orientated ORFs in ISC1043</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1507</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90309">
        <property type="entry name" value="B90309"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y42"/>
    <dbReference type="PaxDb" id="273057-SSO1507"/>
    <dbReference type="EnsemblBacteria" id="AAK41729">
        <property type="protein sequence ID" value="AAK41729"/>
        <property type="gene ID" value="SSO1507"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1507"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1547"/>
    <dbReference type="eggNOG" id="arCOG09862">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1656966_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y42"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="159" mass="18635" checksum="63B13B82E371F008" modified="2001-10-01" version="1">MYHEHMNKKLTQKDFDNFKESIEDLIKLHKESRLSKIENNKESEEERLARLRRFGRELRLRGGKVRQDQERTREKKQTNPTREGIHTDNQGISRLIQQGNRRTSITTEPNEGGDKPQDSRETILPPHIFVILYNLLSKTASGLKVVMDRTGYSLVVTKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YD6</accession>
    <name>Q97YD6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1388</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90296">
        <property type="entry name" value="B90296"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991620.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YD6"/>
    <dbReference type="PaxDb" id="273057-SSO1388"/>
    <dbReference type="EnsemblBacteria" id="AAK41625">
        <property type="protein sequence ID" value="AAK41625"/>
        <property type="gene ID" value="SSO1388"/>
    </dbReference>
    <dbReference type="GeneID" id="1454404"/>
    <dbReference type="KEGG" id="sso:SSO1388"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1395"/>
    <dbReference type="eggNOG" id="arCOG05901">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_311621_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YD6"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="1031" mass="120698" checksum="9B5FF1A7D55EC3F5" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YH0</accession>
    <name>Q97YH0_SACS2</name>
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        <submittedName>
            <fullName evidence="7">Permease, multidrug efflux</fullName>
        </submittedName>
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    <gene>
        <name evidence="7" type="ordered locus">SSO1351</name>
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    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
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            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
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            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="AAK41587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="D90291"/>
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    </dbReference>
    <dbReference type="STRING" id="273057.SSO1351"/>
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    <dbReference type="PaxDb" id="273057-SSO1351"/>
    <dbReference type="EnsemblBacteria" id="AAK41587">
        <property type="protein sequence ID" value="AAK41587"/>
        <property type="gene ID" value="SSO1351"/>
    </dbReference>
    <dbReference type="GeneID" id="1454366"/>
    <dbReference type="KEGG" id="sso:SSO1351"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1357"/>
    <dbReference type="eggNOG" id="arCOG00144">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="InParanoid" id="Q97YH0"/>
    <dbReference type="PhylomeDB" id="Q97YH0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Permease, multidrug efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Permease, multidrug efflux"/>
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    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF5">
        <property type="entry name" value="TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="539"/>
        </location>
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        <location>
            <begin position="7"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAK41587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="559" mass="60491" checksum="ADC0C903F6DB157F" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YH4</accession>
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        <submittedName>
            <fullName evidence="7">Third ORF in transposon ISC1904</fullName>
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    </protein>
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        <name evidence="7" type="ordered locus">SSO1347</name>
    </gene>
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        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
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        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAK41583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblBacteria" id="AAK41583">
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        <property type="gene ID" value="SSO1347"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1347"/>
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    <dbReference type="HOGENOM" id="CLU_095573_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
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        <property type="entry name" value="Cas12f1-like_TNB"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
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        <location>
            <begin position="3"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="6">
        <location>
            <begin position="70"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="183" mass="21820" checksum="D9D7C37CF525B10B" modified="2001-10-01" version="1">MHQSTRRGRELMRKYSHRERNRVLDFVHKFVNTLLDLYPMTFFAVEKLNKESMFKDANDSLSRKISRTVWRSIHRVLKYKAPLYGSFVKEVNPYLTSRSCPRCRFVSRKVGKTFECERCGFKLDRQLNASLNIYLKMCGFPHIREIARVWVGVIPLMGRRRMNVRDFGEAQGLRVDIKYHEIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YI2</accession>
    <name>Q97YI2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase ISC1316</fullName>
        </submittedName>
    </protein>
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        <name evidence="8" type="ordered locus">SSO1341</name>
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    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
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            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90290">
        <property type="entry name" value="B90290"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923334.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YI2"/>
    <dbReference type="SMR" id="Q97YI2"/>
    <dbReference type="PaxDb" id="273057-SSO1341"/>
    <dbReference type="EnsemblBacteria" id="AAK41577">
        <property type="protein sequence ID" value="AAK41577"/>
        <property type="gene ID" value="SSO1341"/>
    </dbReference>
    <dbReference type="GeneID" id="1454356"/>
    <dbReference type="KEGG" id="sso:SSO1341"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1345"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YI2"/>
    <dbReference type="PhylomeDB" id="Q97YI2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="175"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="300"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="403" mass="47246" checksum="1CA8407466D295C0" modified="2001-10-01" version="1">MPTLGFRFRAYTDEQTLRALKAQLKLACKIYNTLRWADIYFYQRDGKGLTQTELRQLALDLRKQDDEYKQLYSQVVQQIADRYYEARQRFFEGLARFPKEKKPHKYYSLVYPQYGWKILHVREIRKGSKKKLITLKLSNLGTFKVIVHRDFPLDKVKRVVVKLTRSERIYITFVVDHEFPKLPNTGKVVAIDVGVEKLLVTSDGEYFPNLRPYEKALWKVKHLHRELSRKKFLSNNWFKAKVKLARAYEYLKNLRTDLYMKLGKWFAEHYDVVVMEGIHAKQLVGKSLRSLRRRLSDVGFGELRGVLKYQLEKYGKKLILVNPAYTSKTCARCGYVKNDLSLSDRVFVCPNCGWIADRDYNASLNILRGSGSERPLVPVELRPLPVLWQGGAMKQEAPSFMRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YM9</accession>
    <name>Q97YM9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1293</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90284">
        <property type="entry name" value="D90284"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YM9"/>
    <dbReference type="PaxDb" id="273057-SSO1293"/>
    <dbReference type="EnsemblBacteria" id="AAK41531">
        <property type="protein sequence ID" value="AAK41531"/>
        <property type="gene ID" value="SSO1293"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1293"/>
    <dbReference type="eggNOG" id="arCOG07929">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2243979_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YM9"/>
    <dbReference type="PhylomeDB" id="Q97YM9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="104" mass="12123" checksum="0FD48BCC3DE4085C" modified="2001-10-01" version="1">MPKDQVLKFYNKVRNPIETSYKNIKSFLPFISLIKFASILEKFLDYDLVVYPLPKAINFLKALAFRKLIFMIALYPLLWLQKITATVHFVRGEACGASSQYSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YR4</accession>
    <name>Q97YR4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1255</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90279">
        <property type="entry name" value="G90279"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991884.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YR4"/>
    <dbReference type="STRING" id="273057.SSO1255"/>
    <dbReference type="PaxDb" id="273057-SSO1255"/>
    <dbReference type="EnsemblBacteria" id="AAK41494">
        <property type="protein sequence ID" value="AAK41494"/>
        <property type="gene ID" value="SSO1255"/>
    </dbReference>
    <dbReference type="GeneID" id="1454273"/>
    <dbReference type="KEGG" id="sso:SSO1255"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1250"/>
    <dbReference type="eggNOG" id="arCOG06044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_5_1_2"/>
    <dbReference type="InParanoid" id="Q97YR4"/>
    <dbReference type="PhylomeDB" id="Q97YR4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="entry name" value="GntR ligand-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43537">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, GNTR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43537:SF5">
        <property type="entry name" value="UXU OPERON TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="GntR ligand-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="212" mass="24275" checksum="4DB187FD7B792118" modified="2001-10-01" version="1">MSLSQIAYEKILEYIITGKYKPGNTLKEEELASLLKISRTPIREALARLEKEGVIVKNGKSFTVIPLTESDILQLYEVRINLESLSAKLAAIRASQDEINKIINVLNEIRGSANADPLTLANLNGNLHRAIAEASHNKYIVDILDSIRLKLKIVRITLFTSFQRRDEEFREHESIVIAIKNRSPDLAYDMMRMHEEKVLDYVKQNILPVMFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YU0</accession>
    <name>Q97YU0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">First ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1217</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="A99276"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="PaxDb" id="273057-SSO1217"/>
    <dbReference type="EnsemblBacteria" id="AAK41464">
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        <property type="gene ID" value="SSO1217"/>
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    <dbReference type="KEGG" id="sso:SSO1217"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1217"/>
    <dbReference type="eggNOG" id="arCOG03164">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
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        <property type="component" value="Chromosome"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
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    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
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        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative DNA-binding domain"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="5">
        <location>
            <begin position="55"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="195" mass="22692" checksum="FB9CF5BAC15E4D83" modified="2001-10-01" version="1">MLRPKEACQRLGISYATLREYVKKGYIKPVILQSGKQRFGEEDVERLMGIIRKRKVILYARVSSSTQKDELVNQVKYLEEQVKEYDLVITDIGSGLNMKRKGFLKLLRMILNNEVSRVVVAYPDRLVRVGFEILEEVCKAHNCEIVVLNQEDKEEELVEDLMSALVSFSGKLYGMRSHEYEKVKKCAEELKNWKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YV8</accession>
    <name>Q97YV8_SACS2</name>
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        <submittedName>
            <fullName evidence="3">Phosphohistidine phosphatase (SixA)</fullName>
            <ecNumber evidence="3">3.1.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">sixA</name>
        <name evidence="3" type="ordered locus">SSO1195</name>
    </gene>
    <organism evidence="3 4">
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        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.-" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="D90273"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YV8"/>
    <dbReference type="FunCoup" id="Q97YV8">
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    </dbReference>
    <dbReference type="STRING" id="273057.SSO1195"/>
    <dbReference type="PaxDb" id="273057-SSO1195"/>
    <dbReference type="EnsemblBacteria" id="AAK41443">
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        <property type="gene ID" value="SSO1195"/>
    </dbReference>
    <dbReference type="GeneID" id="1454222"/>
    <dbReference type="GeneID" id="44130127"/>
    <dbReference type="KEGG" id="sso:SSO1195"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1194"/>
    <dbReference type="eggNOG" id="arCOG01992">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084603_3_1_2"/>
    <dbReference type="InParanoid" id="Q97YV8"/>
    <dbReference type="PhylomeDB" id="Q97YV8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101006">
        <property type="term" value="F:protein histidine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051021">
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00300">
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="161" mass="18180" checksum="862843770B9F113D" modified="2001-10-01" version="1">MITLILVRHGDAEPQVDGKEDKDRKLVKKGVKQMRRVANFLEELGFNVDRIVSSPYLRAYQSAEVILEELFDDNSEKKVETFDDLTPDKEPSLFLEKLKDFVDNSTILVVGHEPYLSSFVKTISGANVEIKKGGVAVLDYDLKEGRGTLKILLSQKVLKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YW1</accession>
    <name>Q97YW1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CHAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90272">
        <property type="entry name" value="H90272"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992800.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YW1"/>
    <dbReference type="STRING" id="273057.SSO1190"/>
    <dbReference type="PaxDb" id="273057-SSO1190"/>
    <dbReference type="EnsemblBacteria" id="AAK41439">
        <property type="protein sequence ID" value="AAK41439"/>
        <property type="gene ID" value="SSO1190"/>
    </dbReference>
    <dbReference type="GeneID" id="1454218"/>
    <dbReference type="KEGG" id="sso:SSO1190"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1190"/>
    <dbReference type="eggNOG" id="arCOG07254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1329527_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YW1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="24744" checksum="BCBCA0D7016481BD" modified="2001-10-01" version="1">MTISQVKDYLNLQLKKAIQINGIGVEEIHDMRVAVRKYFDVLYAIHPVYENVECLFLAKEAIKRLGKVRDMDICEIANGERTKLAIRALKDVRELQVCFVNDKIYGVRLTIYNRILSSLHQIQDITDFHELRKNIRVTRNLVEALGYDNTEIKALAKKMGDIRDEILKMRCRGLTPPDINIIQYKEEAKRVILKIIASQEEFHHFKIEDRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YY0</accession>
    <name>Q97YY0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycoside hydrolase family 57 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1172</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glycosyl hydrolase 57 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90270">
        <property type="entry name" value="E90270"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991569.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YY0"/>
    <dbReference type="STRING" id="273057.SSO1172"/>
    <dbReference type="CAZy" id="GH57">
        <property type="family name" value="Glycoside Hydrolase Family 57"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1172"/>
    <dbReference type="EnsemblBacteria" id="AAK41420">
        <property type="protein sequence ID" value="AAK41420"/>
        <property type="gene ID" value="SSO1172"/>
    </dbReference>
    <dbReference type="GeneID" id="1454199"/>
    <dbReference type="KEGG" id="sso:SSO1172"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1165"/>
    <dbReference type="eggNOG" id="arCOG03284">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_321236_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YY0"/>
    <dbReference type="BRENDA" id="3.2.1.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10796">
        <property type="entry name" value="GH57N_APU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052046">
        <property type="entry name" value="GH57_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004300">
        <property type="entry name" value="Glyco_hydro_57_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017002">
        <property type="entry name" value="Glyco_hydro_57_Saci1162_prd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36306:SF1">
        <property type="entry name" value="ALPHA-AMYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36306">
        <property type="entry name" value="ALPHA-AMYLASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03065">
        <property type="entry name" value="Glyco_hydro_57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032656">
        <property type="entry name" value="Glycosyl_Ohase_Saci_1162_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="876"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 57 N-terminal" evidence="5">
        <location>
            <begin position="348"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="902" mass="101481" checksum="A0E641524B90874A" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z17</accession>
    <name>Q97Z17_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxiredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2007-07" db="PDB data bank">
            <title>Crystal structure of protein of unknown function (NP_342590.1) from Sulfolobus solfataricus at 2.09 A resolution.</title>
            <authorList>
                <consortium name="Joint Center for Structural Genomics (JCSG)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.09 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90265">
        <property type="entry name" value="E90265"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989756.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2QS7">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.09 A"/>
        <property type="chains" value="A/B/C/D=1-143"/>
    </dbReference>
    <dbReference type="PDBsum" id="2QS7"/>
    <dbReference type="AlphaFoldDB" id="Q97Z17"/>
    <dbReference type="SMR" id="Q97Z17"/>
    <dbReference type="FunCoup" id="Q97Z17">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1126"/>
    <dbReference type="PaxDb" id="273057-SSO1126"/>
    <dbReference type="EnsemblBacteria" id="AAK41380">
        <property type="protein sequence ID" value="AAK41380"/>
        <property type="gene ID" value="SSO1126"/>
    </dbReference>
    <dbReference type="GeneID" id="1454159"/>
    <dbReference type="KEGG" id="sso:SSO1126"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1120"/>
    <dbReference type="eggNOG" id="arCOG02064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094970_2_0_2"/>
    <dbReference type="InParanoid" id="Q97Z17"/>
    <dbReference type="PhylomeDB" id="Q97Z17"/>
    <dbReference type="EvolutionaryTrace" id="Q97Z17"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032836">
        <property type="entry name" value="DsrE2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655">
        <property type="entry name" value="CONSERVED WITHIN P. AEROPHILUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655:SF2">
        <property type="entry name" value="PEROXIREDOXIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13686">
        <property type="entry name" value="DrsE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="3" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2QS7"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="16112" checksum="FAD5AF90B0967CAF" modified="2001-10-01" version="1">MAEEKKKKLSIIVFSGTIDKLMPVGILTSGAAASGYEVNLFFTFWGLQAITKRSLNSQQPPQIDKNYEQMGPIMMQKMQEMKYPMWHQLVQQAKEIGEVKVFACSTTMEFFGIKREDLAEFVDDVVGVATFLDRAEGGTTLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z26</accession>
    <name>Q97Z26_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Biotin synthase (BioB)</fullName>
            <ecNumber evidence="6">2.8.1.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">bioB</name>
        <name evidence="6" type="ordered locus">SSO1115</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.1.6" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90264">
        <property type="entry name" value="D90264"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989747.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z26"/>
    <dbReference type="FunCoup" id="Q97Z26">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1115"/>
    <dbReference type="PaxDb" id="273057-SSO1115"/>
    <dbReference type="EnsemblBacteria" id="AAK41371">
        <property type="protein sequence ID" value="AAK41371"/>
        <property type="gene ID" value="SSO1115"/>
    </dbReference>
    <dbReference type="GeneID" id="1454150"/>
    <dbReference type="KEGG" id="sso:SSO1115"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1111"/>
    <dbReference type="eggNOG" id="arCOG00662">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054041_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z26"/>
    <dbReference type="PhylomeDB" id="Q97Z26"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01098">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="30"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="351" mass="39814" checksum="892DB90BC987F752" modified="2001-10-01" version="1">MTLRLISSPDWVRLSFGADMVLGFSSGVFLKGALNTTINLLQYYPDGCKANCSYCGQAREVANGPECKTLIRVEWPLRPLNEVLKRIYERQGNPEYGLQRICIGQLAHPRASPDAIEITRRIRNAGIELQISELVTATYTFKHHMIEMRKAGADMIDVAIDAANEKVFEELRGKKARSMHSWKRYLEAIDEAVEVFGKKNAGIHLIIGLGESEKDAVNLMWYAHSRGAKISLFAFYPEAGTPMEKRKPVPVHVYRRMQIARWLIENDIVDINAFKFDDKGELIDVEIPSDITLNELAPAFMTSGCPGCNRPYSNERPGRVLKNVPWYLDREMTLRSIKASRLDSLIKKVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z59</accession>
    <name>Q97Z59_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DSBA oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1074</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90260">
        <property type="entry name" value="A90260"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923189.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z59"/>
    <dbReference type="STRING" id="273057.SSO1074"/>
    <dbReference type="PaxDb" id="273057-SSO1074"/>
    <dbReference type="EnsemblBacteria" id="AAK41336">
        <property type="protein sequence ID" value="AAK41336"/>
        <property type="gene ID" value="SSO1074"/>
    </dbReference>
    <dbReference type="GeneID" id="1454115"/>
    <dbReference type="KEGG" id="sso:SSO1074"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1074"/>
    <dbReference type="eggNOG" id="arCOG02869">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102827_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z59"/>
    <dbReference type="PhylomeDB" id="Q97Z59"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887:SF14">
        <property type="entry name" value="DISULFIDE BOND FORMATION PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13743">
        <property type="entry name" value="Thioredoxin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="26089" checksum="2AA49B0D10163C4F" modified="2001-10-01" version="1">MAIKIVFFHDVLCPFCFVTSRRLRKVVNKFKEEVVVKHKAFMIISSLEDLKAAAPTEEEAKEIFKQEFSIIKRYYPDYDPEKVIGKGKIIWVWSLPPLIACKAAEYQRGDNGYWDYFDKAQEKFFLEGENVNDDNILIQIAEELGLDVEKFKEDFKSKKARMSVFEDEAEAHAMGIRGVPALLVNDYWLIRGVQEETYLESVIEDLLSNGGEPKKVKLKAYWEAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z98</accession>
    <name>Q97Z98_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sulfate ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">SSO1032</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90254">
        <property type="entry name" value="H90254"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989205.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z98"/>
    <dbReference type="FunCoup" id="Q97Z98">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1032"/>
    <dbReference type="PaxDb" id="273057-SSO1032"/>
    <dbReference type="EnsemblBacteria" id="AAK41295">
        <property type="protein sequence ID" value="AAK41295"/>
        <property type="gene ID" value="SSO1032"/>
    </dbReference>
    <dbReference type="GeneID" id="84058574"/>
    <dbReference type="KEGG" id="sso:SSO1032"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1024"/>
    <dbReference type="eggNOG" id="arCOG00164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_14_1_2"/>
    <dbReference type="InParanoid" id="Q97Z98"/>
    <dbReference type="PhylomeDB" id="Q97Z98"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30183">
        <property type="entry name" value="MOLYBDENUM TRANSPORT SYSTEM PERMEASE PROTEIN MODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30183:SF3">
        <property type="entry name" value="MOLYBDENUM TRANSPORT SYSTEM PERMEASE PROTEIN MODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="52"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK41295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="265" mass="29319" checksum="9126310A9D252D93" modified="2001-10-01" version="1">MRVISLKFNTLQAISFFLSLLLALPTLYLLFYGYGPFFVKSVAFSSSLLSSIGLSFFASALSIVITVLIFTPLAYYLSRHRNPIIETLVDVPASIPHPLVGIALIFIDSPTNPLGALLYSHGIIFYYTYTGLLLALIIVSSPIYIRSMQNFFESLPRSYEIYAMSLGASEFKVFTSVVFPLSIRGIISAGLTSIARAISEFGSVVIVAPYVTGWLFNNAYVASVYIYNEYETYFSASISASATLLLFSLILIVTSRIVNHFLYKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZA2</accession>
    <name>Q97ZA2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90254">
        <property type="entry name" value="D90254"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989211.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZA2"/>
    <dbReference type="STRING" id="273057.SSO1028"/>
    <dbReference type="PaxDb" id="273057-SSO1028"/>
    <dbReference type="EnsemblBacteria" id="AAK41291">
        <property type="protein sequence ID" value="AAK41291"/>
        <property type="gene ID" value="SSO1028"/>
    </dbReference>
    <dbReference type="GeneID" id="84058578"/>
    <dbReference type="KEGG" id="sso:SSO1028"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1020"/>
    <dbReference type="eggNOG" id="arCOG00891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031506_5_2_2"/>
    <dbReference type="InParanoid" id="Q97ZA2"/>
    <dbReference type="PhylomeDB" id="Q97ZA2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46124">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46124:SF2">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR005902-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="232" mass="26580" checksum="687D100EF6F253C0" modified="2001-10-01" version="1">MYKYFDAHCHYSYLKKKYEGYLIAAVSMDYQSAMDTFSLKSDNVLVGVGIHPWRVHEEDLTRILSLIIKADFVGEVGLDYRFAKAPKELQIKYFEEQIKAGEGKLINVHALDAWKDAFNILVKNDVKSAIFHWYTGPINLLKDIESAGYFISINPSVSFQKKHQEVLKNVDLKNVLTESDGGYEYRGKLLEPSDIPNAISFMSNFLGIEEYKLVRIISNNFFKAYGVNPVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZG7</accession>
    <name>Q97ZG7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF373 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0949</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90245">
        <property type="entry name" value="H90245"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992384.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZG7"/>
    <dbReference type="STRING" id="273057.SSO0949"/>
    <dbReference type="PaxDb" id="273057-SSO0949"/>
    <dbReference type="EnsemblBacteria" id="AAK41223">
        <property type="protein sequence ID" value="AAK41223"/>
        <property type="gene ID" value="SSO0949"/>
    </dbReference>
    <dbReference type="GeneID" id="1455190"/>
    <dbReference type="KEGG" id="sso:SSO0949"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.944"/>
    <dbReference type="eggNOG" id="arCOG04151">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048986_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZG7"/>
    <dbReference type="PhylomeDB" id="Q97ZG7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007254">
        <property type="entry name" value="DUF373"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38815:SF1">
        <property type="entry name" value="DUF373 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38815">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF373 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04123">
        <property type="entry name" value="DUF373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="355" mass="39808" checksum="107EEF8530FFE869" modified="2001-10-01" version="1">MVKTIVIYVDIDDDIGDLGFSTPIIGEEKCKLTLDKAAYFMPTDSDFNSMVVAFNIYTDLKRKGEDTEIVFISGSKKGGTESQLEFSKKLDKVIEELSPEYAVVVYDSPDDARAIPIIQSRLKISAVQQVIVEQYRGVEETYMLLAKYIRKAVTEKRYARVFLGVPGIILALAGILAALNLSVYIEPTILIILGVAMVIKGLKIDDLIESWWENSTIMVITATVSIISLLIGVINLYIQLQIVRGLSSIQEFAFIILQILPYASFSAIILFGGKAISKGLNRDIRVWHDIIKIINIVIIYFILFTVVKDIIDNTYILTFQSLYTLILASIVIISIYITLTALEKYGILEKLIKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZL7</accession>
    <name>Q97ZL7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartokinase</fullName>
            <ecNumber evidence="7">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">akH</name>
        <name evidence="10" type="ordered locus">SSO0876</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-aspartate + ATP = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the aspartokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90238">
        <property type="entry name" value="D90238"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992294.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZL7"/>
    <dbReference type="FunCoup" id="Q97ZL7">
        <property type="interactions" value="249"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0876"/>
    <dbReference type="PaxDb" id="273057-SSO0876"/>
    <dbReference type="DNASU" id="1455130"/>
    <dbReference type="EnsemblBacteria" id="AAK41163">
        <property type="protein sequence ID" value="AAK41163"/>
        <property type="gene ID" value="SSO0876"/>
    </dbReference>
    <dbReference type="GeneID" id="1455130"/>
    <dbReference type="KEGG" id="sso:SSO0876"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.884"/>
    <dbReference type="eggNOG" id="arCOG00861">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_6_1_2"/>
    <dbReference type="InParanoid" id="Q97ZL7"/>
    <dbReference type="PhylomeDB" id="Q97ZL7"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00015"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00461"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009090">
        <property type="term" value="P:homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04892">
        <property type="entry name" value="ACT_AK-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04921">
        <property type="entry name" value="ACT_AKi-HSDH-ThrA-like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.10">
        <property type="entry name" value="VC0802-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054352">
        <property type="entry name" value="ACT_Aspartokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005260">
        <property type="entry name" value="Asp_kin_monofn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499">
        <property type="entry name" value="ASPARTATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499:SF70">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22468">
        <property type="entry name" value="ACT_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000726">
        <property type="entry name" value="Asp_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="9">
        <location>
            <begin position="313"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK41163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="446" mass="48765" checksum="0B264F50395E43C1" modified="2001-10-01" version="1">MALIVKIGGSIQKDEKDYELIVKKIQDFSKKSDKIIVVTSAIKNVTNELISATSNTDNSPNIVTEIYERHIKLLSKLADGKEFENSFKDISRLSDELFRVAWSIRVLDEVTPRVRDYILSFGERMATLLLSAILRSNGIEAEGIITPPFLTDENYGEANVIEDLSKNEIANILENAKANVIVLPGFIGRTREGRYTTLGRGGSDYTATLLGKLVGVREVRLVTEVPGIMTGDPKKFENAKTISRLSLEEAIELSQLGAKRLHPRTFDPVFGSDMKVIVESLYEDGFTIINGECQNNDGLKGISLLDNAKLITVESTKIVGKIGSAARITNEAKEAGVNIISISQPASETTIQLVVDSLSANRLLSKLEEIRGTLVKDIEVNDINIVGIVGCGIKKKEISTKVLSIASSYDPLAISRGISNVSMTFIVNKDEGEKLAKELHKVIVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZL9</accession>
    <name>Q97ZL9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase/histidinol phosphatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90238">
        <property type="entry name" value="B90238"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992292.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZL9"/>
    <dbReference type="STRING" id="273057.SSO0873"/>
    <dbReference type="PaxDb" id="273057-SSO0873"/>
    <dbReference type="EnsemblBacteria" id="AAK41161">
        <property type="protein sequence ID" value="AAK41161"/>
        <property type="gene ID" value="SSO0873"/>
    </dbReference>
    <dbReference type="GeneID" id="1455128"/>
    <dbReference type="KEGG" id="sso:SSO0873"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.882"/>
    <dbReference type="eggNOG" id="arCOG00302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072983_1_0_2"/>
    <dbReference type="InParanoid" id="Q97ZL9"/>
    <dbReference type="PhylomeDB" id="Q97ZL9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07432">
        <property type="entry name" value="PHP_HisPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000101">
        <property type="entry name" value="PHP domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052018">
        <property type="entry name" value="PHP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42924">
        <property type="entry name" value="EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42924:SF3">
        <property type="entry name" value="POLYMERASE_HISTIDINOL PHOSPHATASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13263">
        <property type="entry name" value="PHP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase/histidinol phosphatase N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="219" mass="25051" checksum="8A23D2F8DF696649" modified="2001-10-01" version="1">MFFDLHVHSRYSDGKYLPKDIIAYAKAKHIHVAITDHDTSLGLNTVKEEKVIPGQEVTTEYGHVVILCNFPPSPPNRIAELVDYAKENSCVVFPSHPFDIFRKGIGDKTFRYKFDLIEIYNSKAPKMANNKAKEASIRLNLPGVSNSDAHVIQAIGSAYNDLYEIIEFNLDDILDNLRRGKIRNIINGLSFRAKFSILQWYIERKIRNAQNSSRTMHQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZS0</accession>
    <name>Q97ZS0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1173</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0509</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90196">
        <property type="entry name" value="F90196"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZS0"/>
    <dbReference type="PaxDb" id="273057-SSO0509"/>
    <dbReference type="EnsemblBacteria" id="AAK40829">
        <property type="protein sequence ID" value="AAK40829"/>
        <property type="gene ID" value="SSO0509"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0509"/>
    <dbReference type="eggNOG" id="arCOG02133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881169_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZS0"/>
    <dbReference type="PhylomeDB" id="Q97ZS0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="135" mass="15454" checksum="DA61BF6B761F2FE6" modified="2001-10-01" version="1">MNLVALAQLILFVLRNLNLKPRKHKPEEIALAIAAYVMGVQITKLNIPPSTLYYYTKKLGVKRRKDARPRCPSCNSDSTLRFSPPLEQFFDPTRLTVNTFYQSGQPFGPEYQTRGVLEVLDNEEREPSRPRSLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZU6</accession>
    <name>Q97ZU6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HAD family hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0481</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90193">
        <property type="entry name" value="C90193"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922996.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZU6"/>
    <dbReference type="FunCoup" id="Q97ZU6">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0481"/>
    <dbReference type="PaxDb" id="273057-SSO0481"/>
    <dbReference type="EnsemblBacteria" id="AAK40802">
        <property type="protein sequence ID" value="AAK40802"/>
        <property type="gene ID" value="SSO0481"/>
    </dbReference>
    <dbReference type="GeneID" id="15298025"/>
    <dbReference type="KEGG" id="sso:SSO0481"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.475"/>
    <dbReference type="eggNOG" id="arCOG02293">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_19_3_2"/>
    <dbReference type="InParanoid" id="Q97ZU6"/>
    <dbReference type="PhylomeDB" id="Q97ZU6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051806">
        <property type="entry name" value="HAD-like_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481">
        <property type="entry name" value="FRUCTOSE-1-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481:SF4">
        <property type="entry name" value="GLYCEROL-1-PHOSPHATE PHOSPHOHYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="23521" checksum="05758CA870A696A3" modified="2001-10-01" version="1">MRKAVIFDLDGTLANTDEVHKLAWEIALRKLGYDVKVDIRYLLGRKTIDIAKILIGESHAEKLASVKTEIYNELVKTLAKPKPCVMELINRLRDVEIPIAVVTSSMRNSAVQVLKVINVEPDVLITGDDVKLGKPNPMPVIEAIKRLNVDPNESVGVGDTIYDVMAYYSAGIREIIVVRSSVPLNVEEARKYDAKILDSLCEISDWFELF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-16" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZX0</accession>
    <name>SYW_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Tryptophanyl-tRNA synthetase</fullName>
            <shortName evidence="1">TrpRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpS</name>
        <name type="ordered locus">SSO0452</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Trp) + L-tryptophan + ATP = L-tryptophyl-tRNA(Trp) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40778" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40778.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90190">
        <property type="entry name" value="C90190"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988707.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZX0"/>
    <dbReference type="SMR" id="Q97ZX0"/>
    <dbReference type="FunCoup" id="Q97ZX0">
        <property type="interactions" value="344"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0452"/>
    <dbReference type="PaxDb" id="273057-SSO0452"/>
    <dbReference type="EnsemblBacteria" id="AAK40778">
        <property type="protein sequence ID" value="AAK40778"/>
        <property type="gene ID" value="SSO0452"/>
    </dbReference>
    <dbReference type="GeneID" id="1455588"/>
    <dbReference type="KEGG" id="sso:SSO0452"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.447"/>
    <dbReference type="eggNOG" id="arCOG01887">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032621_0_1_2"/>
    <dbReference type="InParanoid" id="Q97ZX0"/>
    <dbReference type="PhylomeDB" id="Q97ZX0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000007">
        <property type="entry name" value="Tryptophan--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000138">
        <property type="entry name" value="Tryptophan--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_A">
        <property type="entry name" value="Trp_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020653">
        <property type="entry name" value="Tryptophan-tRNA-ligase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008924">
        <property type="entry name" value="PRK12285.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008927">
        <property type="entry name" value="PRK12285.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055:SF1">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055">
        <property type="entry name" value="TRYPTOPHANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000136733" description="Tryptophan--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="81"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="253"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="380" mass="44691" checksum="CF8344CF63883680" modified="2002-04-16" version="2">MPDEFTVTPWEVKGKVDYDKLIVQFGTQKITEELKQRIKNLAGDLHVMLRRNVFFSHRDLDLVLNDYEKSKGFFLYTGRAPSLGMHIGHLIPFIFTKWLQEKFNANLYIEITDDEKYMRNPEFTLDQTRSWAYDNILDIIAVGFNPDKTFIFQDTEYIRNMYPITVKIAKKLTFSEVRATFGLDASSNIGLIFYPALQIAPTMFEKKRCLIPAGIDQDPYWRLQRDIAESLGYYKAAQIHSKFLPPLTGPEGKMSSSNPETAIYLVDDPKTVERKIMKYAFSGGQPTIELHRKYGGNPEIDVPFQWLYYFFEEDDNRIKEIEEEYRSGKMLTGELKQILIDKLNNFLEEHRRRREEAKELVHVFKYDGKLAKQMWEKIHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZX4</accession>
    <name>Q97ZX4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidinol-phosphate transaminase</fullName>
            <ecNumber evidence="3">2.6.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">hisC-like</name>
        <name evidence="11" type="ordered locus">SSO0448</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90189">
        <property type="entry name" value="G90189"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988713.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZX4"/>
    <dbReference type="SMR" id="Q97ZX4"/>
    <dbReference type="FunCoup" id="Q97ZX4">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0448"/>
    <dbReference type="PaxDb" id="273057-SSO0448"/>
    <dbReference type="DNASU" id="1455584"/>
    <dbReference type="EnsemblBacteria" id="AAK40774">
        <property type="protein sequence ID" value="AAK40774"/>
        <property type="gene ID" value="SSO0448"/>
    </dbReference>
    <dbReference type="GeneID" id="1455584"/>
    <dbReference type="KEGG" id="sso:SSO0448"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.442"/>
    <dbReference type="eggNOG" id="arCOG04273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_2_2"/>
    <dbReference type="InParanoid" id="Q97ZX4"/>
    <dbReference type="PhylomeDB" id="Q97ZX4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050106">
        <property type="entry name" value="HistidinolP_aminotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43643:SF6">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43643">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 11" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="10">
        <location>
            <begin position="123"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK40774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="326" mass="37541" checksum="B2DBC4E048BCB7E6" modified="2001-10-01" version="1">MKHGGYPWKNGKPALDVKDFSVNLNPMGVPKFIEELINEAVKLKVYAYYPPQNLREIKGIIAEIYGVNEDLVGVFNGASEVISILDENFTVPQPNYSEYKFHSYYFAEEHEDEFVFELNPGRILTSNPNNPTGSLIKLDEIEEFLINEKNELILDESFIDISLAESATKLVNEFNNLTIINSFTKSLAIPGLRFGFSIGHKSKELEKRVPIWRINSITYYVISNLNPKEVRSFFVNSKIKVKEIYDKVDGVKKLFKMYKSYAPFFLVEFKIPTSLINDELIRRCKCYIRDASNFLGLRNTHARIALKNEVIELINNINEIISEKES</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-06-28" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZZ0</accession>
    <name>IXTPA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">dITP/XTP pyrophosphatase</fullName>
            <ecNumber evidence="1">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="1">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0432</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>XTP + H2O = XMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ITP + H2O = IMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90187">
        <property type="entry name" value="E90187"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988745.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZZ0"/>
    <dbReference type="SMR" id="Q97ZZ0"/>
    <dbReference type="FunCoup" id="Q97ZZ0">
        <property type="interactions" value="240"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0432"/>
    <dbReference type="PaxDb" id="273057-SSO0432"/>
    <dbReference type="EnsemblBacteria" id="AAK40756">
        <property type="protein sequence ID" value="AAK40756"/>
        <property type="gene ID" value="SSO0432"/>
    </dbReference>
    <dbReference type="GeneID" id="1455566"/>
    <dbReference type="KEGG" id="sso:SSO0432"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.424"/>
    <dbReference type="eggNOG" id="arCOG04184">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082080_1_0_2"/>
    <dbReference type="InParanoid" id="Q97ZZ0"/>
    <dbReference type="PhylomeDB" id="Q97ZZ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009143">
        <property type="term" value="P:nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009146">
        <property type="term" value="P:purine nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000001">
        <property type="entry name" value="dITP/XTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01405">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020922">
        <property type="entry name" value="dITP/XTP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="RdgB/HAM1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011396">
        <property type="entry name" value="PRK14821.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00042">
        <property type="entry name" value="RdgB/HAM1 family non-canonical purine NTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067:SF9">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000410429" description="dITP/XTP pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="145"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="173"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01405"/>
        </source>
    </evidence>
    <sequence length="192" mass="21919" checksum="A153E5AE08837F54" modified="2001-10-01" version="1">MLRRGVKIGVLTNNENKFIELKEIAKNFNIELEHLRGEKIEIQSDDLEEISRTAANLAYLIFRRPLIVDDSGLFVQALQNFPGPYTNFVKNTIGLKGILKLLEGIKDRSAYFMTALTFTDGKIIKTFIGIVKGAISEEIRGNLGFGFDPIFIPEGEKRTFAEMSLEEKNRYSHRARAFAKFAEFLESYTEKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-21" modified="2026-01-28" version="146" xmlns="https://uniprot.org/uniprot">
    <accession>Q980A5</accession>
    <name>IF2G_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor 2 subunit gamma</fullName>
            <ecNumber evidence="2 4">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">aIF2-gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">eIF-2-gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">eif2g</name>
        <name type="ordered locus">SSO0412</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
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        <citation type="journal article" date="2006" name="Structure" volume="14" first="119" last="128">
            <title>Structural switch of the gamma subunit in an archaeal aIF2 alpha gamma heterodimer.</title>
            <authorList>
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                <person name="Mechulam Y."/>
                <person name="Blanquet S."/>
                <person name="Schmitt E."/>
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        <citation type="journal article" date="2007" name="J. Mol. Biol." volume="373" first="328" last="336">
            <title>New insights into the interactions of the translation initiation factor 2 from archaea with guanine nucleotides and initiator tRNA.</title>
            <authorList>
                <person name="Nikonov O."/>
                <person name="Stolboushkina E."/>
                <person name="Nikulin A."/>
                <person name="Hasenohrl D."/>
                <person name="Blasi U."/>
                <person name="Manstein D.J."/>
                <person name="Fedorov R."/>
                <person name="Garber M."/>
                <person name="Nikonov S."/>
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        <citation type="journal article" date="2007" name="Proc. Natl. Acad. Sci. U.S.A." volume="104" first="18445" last="18450">
            <title>Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states.</title>
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                <person name="Mechulam Y."/>
                <person name="Blanquet S."/>
                <person name="Schmitt E."/>
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        <citation type="journal article" date="2008" name="J. Mol. Biol." volume="382" first="680" last="691">
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                <person name="Blasi U."/>
                <person name="Manstein D.J."/>
                <person name="Fedorov R."/>
                <person name="Garber M."/>
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                <person name="Coureux P.D."/>
                <person name="Perez J."/>
                <person name="Thompson A."/>
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            <title>Identification of a second GTP-bound magnesium ion in archaeal initiation factor 2.</title>
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</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q980K0</accession>
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            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo11</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1 4">DNA-directed RNA polymerase subunit L</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo11</name>
        <name evidence="1 3" type="synonym">rpoL</name>
        <name type="ordered locus">SSO5577</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5 6 7" key="2">
        <citation type="journal article" date="2008" name="Nature" volume="451" first="851" last="854">
            <title>The X-ray crystal structure of RNA polymerase from Archaea.</title>
            <authorList>
                <person name="Hirata A."/>
                <person name="Klein B.J."/>
                <person name="Murakami K.S."/>
            </authorList>
            <dbReference type="PubMed" id="18235446"/>
            <dbReference type="DOI" id="10.1038/nature06530"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.40 ANGSTROMS) OF THE RNA POLYMERASE COMPLEX</scope>
        <scope>X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS) IN COMPLEX WITH RPO3</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 13-subunit RNA polymerase complex.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-9022031">
            <id>Q980K0</id>
        </interactant>
        <interactant intactId="EBI-9022065">
            <id>P95989</id>
            <label>rpo3</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>2</experiments>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90171">
        <property type="entry name" value="H90171"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990580.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2PA8">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.76 A"/>
        <property type="chains" value="L=1-92"/>
    </dbReference>
    <dbReference type="PDB" id="2PMZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="L/X=1-92"/>
    </dbReference>
    <dbReference type="PDB" id="3HKZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="L/V=1-92"/>
    </dbReference>
    <dbReference type="PDBsum" id="2PA8"/>
    <dbReference type="PDBsum" id="2PMZ"/>
    <dbReference type="PDBsum" id="3HKZ"/>
    <dbReference type="AlphaFoldDB" id="Q980K0"/>
    <dbReference type="SMR" id="Q980K0"/>
    <dbReference type="DIP" id="DIP-60647N"/>
    <dbReference type="FunCoup" id="Q980K0">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="IntAct" id="Q980K0">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO5577"/>
    <dbReference type="PaxDb" id="273057-SSO5577"/>
    <dbReference type="EnsemblBacteria" id="AAK40631">
        <property type="protein sequence ID" value="AAK40631"/>
        <property type="gene ID" value="SSO5577"/>
    </dbReference>
    <dbReference type="GeneID" id="1455441"/>
    <dbReference type="KEGG" id="sso:SSO5577"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.286"/>
    <dbReference type="eggNOG" id="arCOG04111">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090381_5_1_2"/>
    <dbReference type="InParanoid" id="Q980K0"/>
    <dbReference type="PhylomeDB" id="Q980K0"/>
    <dbReference type="BRENDA" id="2.7.7.6">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q980K0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07027">
        <property type="entry name" value="RNAP_RPB11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002233">
        <property type="entry name" value="PRK01146.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF28">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000149339" description="DNA-directed RNA polymerase subunit Rpo11">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="2"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="12"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="38"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="48"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="53"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="61"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18235446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="11427726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="18235446"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="5">
        <source>
            <dbReference type="PDB" id="2PA8"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="3HKZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="2PA8"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <sequence length="92" mass="10285" checksum="B8F3DDA07598088C" modified="2001-10-01" version="1">MEIRILKSESNYLELEIEGEDHTLGNLIAGTLRRISGVSFASYYQPHPLSDKIIVKILTDGSITPKDALLKAIENIRGMTSHYIDEIKGLTK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2021-09-29" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q980L5</accession>
    <name>RPO8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo8</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase, subunit G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo8</name>
        <name evidence="1 3" type="synonym">rpoG</name>
        <name type="ordered locus">SSO0277</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Nature" volume="451" first="851" last="854">
            <title>The X-ray crystal structure of RNA polymerase from Archaea.</title>
            <authorList>
                <person name="Hirata A."/>
                <person name="Klein B.J."/>
                <person name="Murakami K.S."/>
            </authorList>
            <dbReference type="PubMed" id="18235446"/>
            <dbReference type="DOI" id="10.1038/nature06530"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.40 ANGSTROMS) OF THE RNA POLYMERASE COMPLEX</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 6">Part of the 13-subunit RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90169">
        <property type="entry name" value="H90169"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990556.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3HKZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="G/S=1-132"/>
    </dbReference>
    <dbReference type="PDBsum" id="3HKZ"/>
    <dbReference type="AlphaFoldDB" id="Q980L5"/>
    <dbReference type="SMR" id="Q980L5"/>
    <dbReference type="STRING" id="273057.SSO0277"/>
    <dbReference type="PaxDb" id="273057-SSO0277"/>
    <dbReference type="EnsemblBacteria" id="AAK40615">
        <property type="protein sequence ID" value="AAK40615"/>
        <property type="gene ID" value="SSO0277"/>
    </dbReference>
    <dbReference type="GeneID" id="1455425"/>
    <dbReference type="KEGG" id="sso:SSO0277"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.271"/>
    <dbReference type="eggNOG" id="arCOG04271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136588_0_0_2"/>
    <dbReference type="InParanoid" id="Q980L5"/>
    <dbReference type="BRENDA" id="2.7.7.6">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q980L5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00866">
        <property type="entry name" value="RNApol_arch_Rpo8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031555">
        <property type="entry name" value="RNA_pol_Rpo8"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011549">
        <property type="entry name" value="PRK14980.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16992">
        <property type="entry name" value="RNA_pol_RpbG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000453881" description="DNA-directed RNA polymerase subunit Rpo8">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="37"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="54"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="91"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="97"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="111"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18235446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="11427726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="3HKZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="3HKZ"/>
        </source>
    </evidence>
    <sequence length="132" mass="14968" checksum="F9796F096A7831F2" modified="2001-10-01" version="1">MMESVAQEIILSCEINSIERGSLKNLSMVNMSCNGFNVSFDIIDSINIFSQKEKVKVIISKNRPSYSHDDFCGHGYIVTELKDSSLNNGNKYTTIISLYGLLVKIISNKESFLRTSQLNIMDHVYFCVKKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q980P8</accession>
    <name>Q980P8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13 14">Adenylosuccinate lyase</fullName>
            <shortName evidence="14">ASL</shortName>
            <ecNumber evidence="5 13 14">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 14">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">purB</name>
        <name evidence="17" type="ordered locus">SSO0240</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes two reactions in de novo purine nucleotide biosynthesis. Catalyzes the breakdown of 5-aminoimidazole- (N-succinylocarboxamide) ribotide (SAICAR or 2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate) to 5-aminoimidazole-4-carboxamide ribotide (AICAR or 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) and fumarate, and of adenylosuccinate (ADS or N(6)-(1,2-dicarboxyethyl)-AMP) to adenosine monophosphate (AMP) and fumarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:23921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = fumarate + AMP</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:16854"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 14">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="5 13 14"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90165">
        <property type="entry name" value="E90165"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990495.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980P8"/>
    <dbReference type="FunCoup" id="Q980P8">
        <property type="interactions" value="380"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0240"/>
    <dbReference type="PaxDb" id="273057-SSO0240"/>
    <dbReference type="EnsemblBacteria" id="AAK40580">
        <property type="protein sequence ID" value="AAK40580"/>
        <property type="gene ID" value="SSO0240"/>
    </dbReference>
    <dbReference type="GeneID" id="1455390"/>
    <dbReference type="GeneID" id="44129210"/>
    <dbReference type="KEGG" id="sso:SSO0240"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.235"/>
    <dbReference type="eggNOG" id="arCOG01747">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030949_0_1_2"/>
    <dbReference type="InParanoid" id="Q980P8"/>
    <dbReference type="PhylomeDB" id="Q980P8"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01360">
        <property type="entry name" value="Adenylsuccinate_lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000007">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000008">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172:SF1">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 14 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 14" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adenylosuccinate lyase C-terminal" evidence="16">
        <location>
            <begin position="376"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="351"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="NCBIfam" id="TIGR00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AAK40580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="474" mass="53848" checksum="0BF91C5516ADCA30" modified="2001-10-01" version="1">MRLQYKLINVEHTLQLAMEDSICPLEWRYGSNEMRKFFSREEILKRRIQVEIALLKALTKYGLVKEEDVQKVEKASLTIRPEEVDELESKIGHDVMAMVVLMAERSGDSGRYVHFGATSYDIVDTAYALVFRDAIRLVKTKVVEILEMMVSLAIKYKDLAMVGRTHGQHALPITLGFKIANYIYEFSRSLERLNEVEKRIVKGKMAGAVGTMAAWGNIGLEIEENVMKFLGLRPHEISTQVAPRDAFAELTSDLAILASQCDRFALEVRELMRPEINEISEGVEGRVGSSTMPQKENPVTSEKIGGLAKVLRSLVVASLENIPLWHERDLTNSSSERIIISHEFLVIDEILDSMISLLRNLRVNFENIEKNLNLTKGQIMAESLMINLTLKGMKRHEAHQYVNKLTSKVRQTGKTLLEVCLEDSLILSYFSKQELENVLNPKNYLGSYDKLIERAISYAKGVIKGSEIREAQDR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Q3</accession>
    <name>GATB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
            <shortName>Asp/Glu-ADT subunit B</shortName>
            <ecNumber>6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">gatB</name>
        <name type="ordered locus">SSO0232</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>L-aspartyl-tRNA(Asn) + L-glutamine + ATP + H2O = L-asparaginyl-tRNA(Asn) + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="frameshift">
            <sequence resource="EMBL-CDS" id="AAK40575" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.3.5.-"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40575.1"/>
        <property type="status" value="ALT_FRAME"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90164">
        <property type="entry name" value="H90164"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990489.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Q3"/>
    <dbReference type="SMR" id="Q980Q3"/>
    <dbReference type="FunCoup" id="Q980Q3">
        <property type="interactions" value="192"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0232"/>
    <dbReference type="PaxDb" id="273057-SSO0232"/>
    <dbReference type="EnsemblBacteria" id="AAK40575">
        <property type="protein sequence ID" value="AAK40575"/>
        <property type="gene ID" value="SSO0232"/>
    </dbReference>
    <dbReference type="GeneID" id="1455385"/>
    <dbReference type="KEGG" id="sso:SSO0232"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.230"/>
    <dbReference type="eggNOG" id="arCOG01718">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019240_0_1_2"/>
    <dbReference type="InParanoid" id="Q980Q3"/>
    <dbReference type="PhylomeDB" id="Q980Q3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.410:FF:000001">
        <property type="entry name" value="Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="entry name" value="GatB domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="GatB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004012">
        <property type="entry name" value="PRK05477.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004014">
        <property type="entry name" value="PRK05477.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT B MITOCHONDRIAL AND PROKARYOTIC PET112-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148877" description="Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B">
        <location>
            <begin position="1"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="472" mass="53848" checksum="7017A69DAFC15184" modified="2002-11-01" version="2">MVKIGLEVHVHITALKTKLFCSCPSDYTGKDPNTVTCPVCLGLPGAIPVLNENAVKYGILTALALNCQIAEKLIFDRKHYFYPDMSKNYQISQYDGPGSMAIAKDGYIKLNDKIIRIRRINIEEDPAKIVYPTGSILTSKYTLLDYNRSGTPLLEIVTEPDLRSAKEARFFLEKLRSILEHLGVCDCGIEGAMKADVNISVKGGERVEVKNVGSPKDVEDAINYEIARQRASILQGIKIERETRHWDNERRVTVPLRTKETEEDYRYFPDPDLPPYSITPELIEKFKREMPELPDQRAERFVKQYNVTPYQAQVLVNEKALADLFEEITNNYKNYAKVANLLINDYMRWLNENNITVTQSKAKANHIIELFEYLDSGLISIKIVKELLPEMILTGKTPGQLIKEKGMTNIKDENYLERIIDEVLNEEKEAVEKAKRDPKVVNYLVGMVMKKTGKRADPQMTVEIIKKKLGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Y4</accession>
    <name>Q980Y4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial transposase ISC1359</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90154">
        <property type="entry name" value="A90154"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922896.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Y4"/>
    <dbReference type="PaxDb" id="273057-SSO0137"/>
    <dbReference type="EnsemblBacteria" id="AAK40488">
        <property type="protein sequence ID" value="AAK40488"/>
        <property type="gene ID" value="SSO0137"/>
    </dbReference>
    <dbReference type="GeneID" id="1453315"/>
    <dbReference type="KEGG" id="sso:SSO0137"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.130"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067987_0_0_2"/>
    <dbReference type="InParanoid" id="Q980Y4"/>
    <dbReference type="PhylomeDB" id="Q980Y4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033541">
        <property type="entry name" value="transpos_ISH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="89"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="318" mass="36623" checksum="A62AF7C9F37F2963" modified="2001-10-01" version="1">MKHENTTKPAKFNRDFARSALKIIYSVLTKILFPEELLSALLKASGSYLSRLGKDGRRALRKLNAVQVEDVRDALRKMGRMTLRGVRDRRVAVDFHAIPQYHADKSFLSRIKPTKGTSWGLVQAAIFLLGRTRSFLDVIPVTVKNVAEGFKAVMEVIVKELEEDKLRLVIVFADREFAVNEVIRYLLELGLDFVISAKAQMYKKYKGMLQDVDVSFGGVRYTGFLCVRHGSGAYLIILRKEDGKIIAFLVRKEMDLYDAIVLAEMYRERWGIENAFRSLEEFRVRTRTCDVTLSDLSKIKSHYNYNVSYYTILHYIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Z7</accession>
    <name>Q980Z7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO5209</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90151">
        <property type="entry name" value="H90151"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Z7"/>
    <dbReference type="STRING" id="273057.SSO5209"/>
    <dbReference type="PaxDb" id="273057-SSO5209"/>
    <dbReference type="EnsemblBacteria" id="AAK40471">
        <property type="protein sequence ID" value="AAK40471"/>
        <property type="gene ID" value="SSO5209"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO5209"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.111"/>
    <dbReference type="eggNOG" id="arCOG07493">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176733_0_0_2"/>
    <dbReference type="InParanoid" id="Q980Z7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="9921" checksum="3E61B79FB214BD3E" modified="2001-10-01" version="1">MMYEIEMVLNAIKEGAVNPGDAVIKTGLPRYEVLALFHILEELGLIEPIYSKGSHKAFRLTKKGEEILEGLKKGYELYIFVKPNPVQS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-20" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q9P999</accession>
    <name>XYLS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Alpha-xylosidase</fullName>
            <ecNumber>3.2.1.177</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">xylS</name>
        <name type="ordered locus">SSO3022</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="J. Biol. Chem." volume="275" first="22082" last="22089">
            <title>Identification and molecular characterization of the first alpha-xylosidase from an Archaeon.</title>
            <authorList>
                <person name="Moracci M."/>
                <person name="Ponzano B.C."/>
                <person name="Trincone A."/>
                <person name="Fusco S."/>
                <person name="De Rosa M."/>
                <person name="van der Oost J."/>
                <person name="Sensen C.W."/>
                <person name="Charlebois R.L."/>
                <person name="Rossi M."/>
            </authorList>
            <dbReference type="PubMed" id="10801892"/>
            <dbReference type="DOI" id="10.1074/jbc.M910392199"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Catalyzes the liberation of alpha-xylose from the non-reducing terminal glucose of xyloglucan oligosaccharides. Has high hydrolytic activity on the disaccharide isoprimeverose. Follows a retaining mechanism of substrate hydrolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Hydrolysis of terminal, non-reducing alpha-D-xylose residues with release of alpha-D-xylose.</text>
            <dbReference type="EC" id="3.2.1.177"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">17 mM for maltose</KM>
            <KM evidence="2">3.45 mM for maltotriose</KM>
            <KM evidence="2">28.9 mM for isoprimeverose</KM>
            <KM evidence="2">1.72 mM for 4-nitrophenyl-beta-isoprimeveroside</KM>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 5.5.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 90 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.177"/>
    <dbReference type="EMBL" id="AJ251975">
        <property type="protein sequence ID" value="CAB99206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90483">
        <property type="entry name" value="D90483"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992679.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9P999"/>
    <dbReference type="SMR" id="Q9P999"/>
    <dbReference type="STRING" id="273057.SSO3022"/>
    <dbReference type="CAZy" id="GH31">
        <property type="family name" value="Glycoside Hydrolase Family 31"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3022"/>
    <dbReference type="DNASU" id="1453042"/>
    <dbReference type="EnsemblBacteria" id="AAK43123">
        <property type="protein sequence ID" value="AAK43123"/>
        <property type="gene ID" value="SSO3022"/>
    </dbReference>
    <dbReference type="GeneID" id="1453042"/>
    <dbReference type="GeneID" id="44128744"/>
    <dbReference type="KEGG" id="sso:SSO3022"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3115"/>
    <dbReference type="eggNOG" id="arCOG03663">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000631_7_3_2"/>
    <dbReference type="InParanoid" id="Q9P999"/>
    <dbReference type="PhylomeDB" id="Q9P999"/>
    <dbReference type="BioCyc" id="MetaCyc:MONOMER-16613"/>
    <dbReference type="BRENDA" id="3.2.1.177">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q9P999"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061634">
        <property type="term" value="F:alpha-D-xyloside xylohydrolase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06591">
        <property type="entry name" value="GH31_xylosidase_XylS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033403">
        <property type="entry name" value="DUF5110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051816">
        <property type="entry name" value="Glycosyl_Hydrolase_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43863">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G03140)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43863:SF2">
        <property type="entry name" value="MALTASE-GLUCOAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17137">
        <property type="entry name" value="DUF5110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0326">Glycosidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000185369" description="Alpha-xylosidase">
        <location>
            <begin position="1"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="353"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10801892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="731" mass="84485" checksum="85328243A00C4F3E" modified="2000-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX88</accession>
    <name>RL18_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl18</name>
        <name evidence="1" type="synonym">rpl18Ab</name>
        <name type="ordered locus">SSO0699</name>
        <name type="ORF">C10_031</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99218">
        <property type="entry name" value="A99218"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX88"/>
    <dbReference type="SMR" id="Q9UX88"/>
    <dbReference type="FunCoup" id="Q9UX88">
        <property type="interactions" value="233"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0699"/>
    <dbReference type="PaxDb" id="273057-SSO0699"/>
    <dbReference type="EnsemblBacteria" id="AAK41000">
        <property type="protein sequence ID" value="AAK41000"/>
        <property type="gene ID" value="SSO0699"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0699"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.699"/>
    <dbReference type="eggNOG" id="arCOG04088">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056222_2_0_2"/>
    <dbReference type="InParanoid" id="Q9UX88"/>
    <dbReference type="PhylomeDB" id="Q9UX88"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.100:FF:000008">
        <property type="entry name" value="50S ribosomal protein L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_uL18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057267">
        <property type="entry name" value="Rbsml_uL18_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_uL18_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006342">
        <property type="entry name" value="PRK08569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="RIBOSOMAL PROTEIN L5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000131418" description="Large ribosomal subunit protein uL18">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="199" mass="22402" checksum="B7F42036F5C1E557" modified="2000-05-01" version="1">MNKLANGPNYKVKPRRRREGKTNYYKRYVYVISKQTRFIVRITNKYVIVQIAKIDPKGDIMIASAHSAELAKKFGWKGDENNTPAAYLTGYLAGLRAIKRGVTECVADIGLHVPSKGNRVFYVIKGAIDAGLKIPIGDISIENDRIKGEHIAKYAEKLKSENSDLYSKLFSRYLQRGLNPENLPSHFEEILNKIKSSGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-01-11" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXB8</accession>
    <name>PFDB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Prefoldin subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>GimC subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pfdB</name>
        <name type="ordered locus">SSO0730</name>
        <name type="ORF">C20_021</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90221">
        <property type="entry name" value="D90221"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991297.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXB8"/>
    <dbReference type="SMR" id="Q9UXB8"/>
    <dbReference type="FunCoup" id="Q9UXB8">
        <property type="interactions" value="204"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0730"/>
    <dbReference type="PaxDb" id="273057-SSO0730"/>
    <dbReference type="EnsemblBacteria" id="AAK41027">
        <property type="protein sequence ID" value="AAK41027"/>
        <property type="gene ID" value="SSO0730"/>
    </dbReference>
    <dbReference type="GeneID" id="1454994"/>
    <dbReference type="KEGG" id="sso:SSO0730"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.727"/>
    <dbReference type="eggNOG" id="arCOG01342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131909_2_1_2"/>
    <dbReference type="InParanoid" id="Q9UXB8"/>
    <dbReference type="PhylomeDB" id="Q9UXB8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23162">
        <property type="entry name" value="Prefoldin_beta_GimC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000013">
        <property type="entry name" value="Prefoldin subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00307">
        <property type="entry name" value="PfdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012713">
        <property type="entry name" value="PfdB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02338">
        <property type="entry name" value="gimC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000124870" description="Prefoldin subunit beta">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="126" mass="14543" checksum="5895DB741C8DE117" modified="2000-05-01" version="1">MAEKLPPEVQAQLAKFQQLKDQLDRLLLEKSTIENELREINKVLEELSVLNADATIYKIVGNLLVKSDKTSVEKELNDRKELLELRSRTYQKQESILRKQLEDLQAKINDMLSKYYPQGGQTGIKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-03-15" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXE4</accession>
    <name>SPEE_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 5">Polyamine aminopropyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">1,3-diaminopropane aminopropyltransferase</fullName>
            <ecNumber evidence="4">2.5.1.23</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Norspermidine aminopropyltransferase</fullName>
            <ecNumber evidence="4">2.5.1.126</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Norspermine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1 6">Putrescine aminopropyltransferase</fullName>
            <shortName evidence="1">PAPT</shortName>
            <ecNumber evidence="1 4">2.5.1.16</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Spermidine aminopropyltransferase</fullName>
            <ecNumber evidence="4">2.5.1.79</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1 6">Spermidine synthase</fullName>
            <shortName evidence="1">SPDS</shortName>
            <shortName evidence="1">SPDSY</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Sym-norspermidine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Thermospermine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">speE</name>
        <name type="ordered locus">SSO0757</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1986" name="Eur. J. Biochem." volume="161" first="263" last="271">
            <title>Purification and characterization of propylamine transferase from Sulfolobus solfataricus, an extreme thermophilic archaebacterium.</title>
            <authorList>
                <person name="Cacciapuoti G."/>
                <person name="Porcelli M."/>
                <person name="Carteni-Farina M."/>
                <person name="Gambacorta A."/>
                <person name="Zappia V."/>
            </authorList>
            <dbReference type="PubMed" id="3096734"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1986.tb10442.x"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>SUBUNIT</scope>
        <scope>REACTION MECHANISM</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="1992" name="Eur. J. Biochem." volume="204" first="473" last="482">
            <title>Effect of temperature on the propylamine transferase from Sulfolobus solfataricus, an extreme thermophilic archaebacterium. 1. Conformational behavior of the oligomeric enzyme in solution.</title>
            <authorList>
                <person name="Facchiano F."/>
                <person name="Ragone R."/>
                <person name="Porcelli M."/>
                <person name="Cacciapuoti G."/>
                <person name="Colonna G."/>
            </authorList>
            <dbReference type="PubMed" id="1541263"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1992.tb16657.x"/>
        </citation>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="1992" name="Eur. J. Biochem." volume="204" first="483" last="490">
            <title>Effect of temperature on the propylamine transferase from Sulfolobus solfataricus, an extreme thermophilic archaebacterium. 2. Denaturation and structural stability.</title>
            <authorList>
                <person name="Ragone R."/>
                <person name="Facchiano F."/>
                <person name="Cacciapuoti G."/>
                <person name="Porcelli M."/>
                <person name="Colonna G."/>
            </authorList>
            <dbReference type="PubMed" id="1541264"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1992.tb16658.x"/>
        </citation>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the biosynthesis of polyamines which are thought to support the growth of thermophilic microorganisms under high-temperature conditions. It seems that long-chain and branched-chain of polyamines effectively stabilize DNA and RNA, respectively. Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to various amine acceptors such as putrescine (1,4-diaminobutane), 1,3-diaminopropane, sym-norspermidine and spermidine. The biosynthesis of caldopentamine from norspermine has been also observed, but with a very low activity. The reaction involves a nucleophilic attack on the C-3 methylene of the propylamine moiety adjacent to the positively charged sulfur of decarboxy-AdoMet. S-adenosylmethioninamine is the only amino donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12721"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="2.5.1.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-adenosyl 3-(methylsulfanyl)propylamine + propane-1,3-diamine = norspermidine + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23244"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57484"/>
            <dbReference type="ChEBI" id="CHEBI:57920"/>
            <dbReference type="EC" id="2.5.1.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>norspermidine + S-adenosyl 3-(methylsulfanyl)propylamine = norspermine + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42864"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57920"/>
            <dbReference type="ChEBI" id="CHEBI:58704"/>
            <dbReference type="EC" id="2.5.1.126"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = thermospermine + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30515"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:59903"/>
            <dbReference type="EC" id="2.5.1.79"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="4">Competitively inhibited by 5-methylthioadenosine, 5-methylthiotubercidin, S-adenosyl(5)-3-thiopropylamine and S-adenosyl-3-thio-l,8-diaminooctane.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="4">7.9 uM for S-adenosylmethioninamine (at 78 degrees Celsius and at pH 7.5)</KM>
            <KM evidence="4">954 uM for sym-nor-spermidine (at 78 degrees Celsius and at pH 7.5)</KM>
            <KM evidence="2 3 4">1539 uM for spermidine (at 78 degrees Celsius and at pH 7.5)</KM>
            <KM evidence="4">1675 uM for 1,3-diaminopropane (at 78 degrees Celsius and at pH 7.5)</KM>
            <KM evidence="4">3850 uM for putrescine (at 78 degrees Celsius and at pH 7.5)</KM>
            <Vmax evidence="4">1980.0 nmol/min/mg enzyme with 1,3-diaminopropane as substrate (at 78 degrees Celsius and at pH 7.5)</Vmax>
            <Vmax evidence="4">662.0 nmol/min/mg enzyme with putrescine as substrate (at 78 degrees Celsius and at pH 7.5)</Vmax>
            <Vmax evidence="4">316.0 nmol/min/mg enzyme with sym-nor-spermidine as substrate (at 78 degrees Celsius and at pH 7.5)</Vmax>
            <Vmax evidence="4">124.0 nmol/min/mg enzyme with spermidine as substrate (at 78 degrees Celsius and at pH 7.5)</Vmax>
        </kinetics>
        <phDependence>
            <text evidence="4">Optimum pH is 7.5 with one-half of the maximal activity at pH 6 and 9.5.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="4">Optimum temperature is 90 degrees Celsius. No loss of activity is observable even after exposure of the purified enzyme to 100 degrees Celsius for 1 hour. At 60 and 110 degrees Celsius the reaction rate is one-half of the maximal value, and a residual 20% activity is still observable at 120 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.23" evidence="4"/>
    <dbReference type="EC" id="2.5.1.126" evidence="4"/>
    <dbReference type="EC" id="2.5.1.16" evidence="1 4"/>
    <dbReference type="EC" id="2.5.1.79" evidence="4"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90224">
        <property type="entry name" value="D90224"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991336.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXE4"/>
    <dbReference type="SMR" id="Q9UXE4"/>
    <dbReference type="FunCoup" id="Q9UXE4">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0757"/>
    <dbReference type="PaxDb" id="273057-SSO0757"/>
    <dbReference type="EnsemblBacteria" id="AAK41051">
        <property type="protein sequence ID" value="AAK41051"/>
        <property type="gene ID" value="SSO0757"/>
    </dbReference>
    <dbReference type="GeneID" id="1455018"/>
    <dbReference type="GeneID" id="44129755"/>
    <dbReference type="KEGG" id="sso:SSO0757"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.753"/>
    <dbReference type="eggNOG" id="arCOG00050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048199_0_1_2"/>
    <dbReference type="InParanoid" id="Q9UXE4"/>
    <dbReference type="PhylomeDB" id="Q9UXE4"/>
    <dbReference type="UniPathway" id="UPA00248">
        <property type="reaction ID" value="UER00314"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050314">
        <property type="term" value="F:sym-norspermidine synthase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010487">
        <property type="term" value="F:thermospermine synthase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000088">
        <property type="entry name" value="Polyamine aminopropyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43317">
        <property type="entry name" value="THERMOSPERMINE SYNTHASE ACAULIS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43317:SF1">
        <property type="entry name" value="THERMOSPERMINE SYNTHASE ACAULIS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156534" description="Polyamine aminopropyltransferase">
        <location>
            <begin position="1"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="PABS" evidence="1">
        <location>
            <begin position="4"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="141"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="1541263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="1541264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="3096734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="3096734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6">
        <source>
            <dbReference type="PubMed" id="3096734"/>
        </source>
    </evidence>
    <sequence length="301" mass="34854" checksum="2A6A1897C71E773D" modified="2000-05-01" version="1">MFGWHWLLEWQTPYEFHGHLIEKVLAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYHELLVHPLLLSLTKPPKSVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKYLYEWHQGAFEDKRTNLVITDGLKFIKETKEKYDAIILDLTDPIKNSTSYMLYTKEFYEKLREILNERGGIVTQATSPSFSLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGVRPDLLSEDEVDSKINERIDGQLRFYDGYSHKISFSLPKNIKSEFQKITKISTEKDPIYVPA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2025-06-18" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>O33778</accession>
    <name>HIS3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Phosphoribosyl-AMP cyclohydrolase</fullName>
            <shortName>PRA-CH</shortName>
            <ecNumber>3.5.4.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hisI</name>
        <name type="ordered locus">SSO6227</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="J. Bacteriol." volume="179" first="4429" last="4432">
            <title>Evolutionary analysis of the hisCGABdFDEHI gene cluster from the archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Sensen C.W."/>
                <person name="Doolittle W.F."/>
                <person name="Brown J.R."/>
            </authorList>
            <dbReference type="PubMed" id="9209067"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRA-CH family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19"/>
    <dbReference type="EMBL" id="U82227">
        <property type="protein sequence ID" value="AAB63026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90207">
        <property type="entry name" value="A90207"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O33778"/>
    <dbReference type="SMR" id="O33778"/>
    <dbReference type="FunCoup" id="O33778">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO6227"/>
    <dbReference type="PaxDb" id="273057-SSO6227"/>
    <dbReference type="EnsemblBacteria" id="AAK40912">
        <property type="protein sequence ID" value="AAK40912"/>
        <property type="gene ID" value="SSO6227"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO6227"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.608"/>
    <dbReference type="eggNOG" id="arCOG02676">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_5_3_2"/>
    <dbReference type="InParanoid" id="O33778"/>
    <dbReference type="PhylomeDB" id="O33778"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="entry name" value="Phosphoribosyl-AMP cyclohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945:SF1">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="HisI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000136514" description="Phosphoribosyl-AMP cyclohydrolase">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="85" mass="9766" checksum="79C648BED321E372" modified="1998-01-01" version="1">MVGNMNREALFKTLTTGYLHFWSLSRKKLWLKGETSGNFQIIEEFKVDCDADAVLFKVTSLGPICHTGNYTCFYRSYDELVNSKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1991-11-01" modified="2026-01-28" version="137" xmlns="https://uniprot.org/uniprot">
    <accession>P23794</accession>
    <name>GLNA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Glutamine synthetase</fullName>
            <shortName>GS</shortName>
            <ecNumber>6.3.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Glutamate--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">glnA</name>
        <name type="synonym">glnA-1</name>
        <name type="ordered locus">SSO0366</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1990" name="Mol. Gen. Genet." volume="221" first="187" last="194">
            <title>Cloning and nucleotide sequence of an archaebacterial glutamine synthetase gene: phylogenetic implications.</title>
            <authorList>
                <person name="Sanangelantoni A.M."/>
                <person name="Barbarini D."/>
                <person name="di Pasquale G."/>
                <person name="Cammarano P."/>
                <person name="Tiboni O."/>
            </authorList>
            <dbReference type="PubMed" id="1973523"/>
            <dbReference type="DOI" id="10.1007/BF00261719"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2"/>
    <dbReference type="EMBL" id="X53263">
        <property type="protein sequence ID" value="CAA37352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99180">
        <property type="entry name" value="A99180"/>
    </dbReference>
    <dbReference type="PIR" id="S11899">
        <property type="entry name" value="S11899"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990668.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P23794"/>
    <dbReference type="SMR" id="P23794"/>
    <dbReference type="FunCoup" id="P23794">
        <property type="interactions" value="175"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0366"/>
    <dbReference type="PaxDb" id="273057-SSO0366"/>
    <dbReference type="EnsemblBacteria" id="AAK40696">
        <property type="protein sequence ID" value="AAK40696"/>
        <property type="gene ID" value="SSO0366"/>
    </dbReference>
    <dbReference type="GeneID" id="1455506"/>
    <dbReference type="GeneID" id="44129340"/>
    <dbReference type="KEGG" id="sso:SSO0366"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.360"/>
    <dbReference type="eggNOG" id="arCOG01909">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_1_2_2"/>
    <dbReference type="InParanoid" id="P23794"/>
    <dbReference type="PhylomeDB" id="P23794"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006576">
        <property type="term" value="P:biogenic amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019740">
        <property type="term" value="P:nitrogen utilization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000005">
        <property type="entry name" value="Probable glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43407">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43407:SF1">
        <property type="entry name" value="LENGSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000153214" description="Glutamine synthetase">
        <location>
            <begin position="1"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="GS beta-grasp" evidence="2">
        <location>
            <begin position="15"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="3">
        <location>
            <begin position="107"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-AMP-tyrosine" evidence="1">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA37352." evidence="4" ref="1">
        <original>S</original>
        <variation>T</variation>
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="471" mass="53348" checksum="1CC4239287B6C2CD" modified="2001-06-20" version="2">MPSTAEDVLKFLKENNIKWVDLQFTDVPGRLHHITIPADEFDLESLKTGFGKLDGSSIRGFTSIYESDMVLLPVPETMTLIPWSPGVARVLCKVFWGGGKGRFERDPRFIAEEAEKYQTEQGYTSYYGPELEFFMFDKVKLDVSAPQSGTGYKIYAREAPWSDSGTFVIRFKEGYYPAPPVDQLMDVRVEIVDTLVKYFGYTIEATHHEVATAGQGEIDFRFSTLVDTADKVQTLKYVAKNIAAKHGLVVTFMPKPIYGDNGTGMHTHLSLWTKDGKKNLMYDPNDEYAEISQFGRYVIGGLLTHARALSAIVSPTVNSYRRLIPGFEAPVYIAWSKGNRSAVIRVPAYYRGMEKAKRIEYRPPDPSTNPYLAFAALLMAALDGVNKKIDPGDPVDENIYHLTPEKRRQLGIKELPRSLNEALDELESDKEFLKPVFNSSILDTYIDLKRDEARSLQGYPHPMELYYYLDS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1993-07-01" modified="2026-01-28" version="162" xmlns="https://uniprot.org/uniprot">
    <accession>P30925</accession>
    <name>EF2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Elongation factor 2</fullName>
            <shortName>EF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">fusA</name>
        <name type="synonym">fus</name>
        <name type="ordered locus">SSO0728</name>
        <name type="ORF">C10_002</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1993" name="Gene" volume="136" first="41" last="48">
            <title>Cloning and sequencing of the gene encoding thermostable elongation factor 2 in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="de Vendittis E."/>
                <person name="Amatruda M.R."/>
                <person name="Masullo M."/>
                <person name="Bocchini V."/>
            </authorList>
            <dbReference type="PubMed" id="8294039"/>
            <dbReference type="DOI" id="10.1016/0378-1119(93)90445-9"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="1992" name="Biochim. Biophys. Acta" volume="1132" first="127" last="132">
            <title>Molecular, functional and structural properties of an archaebacterial elongation factor 2.</title>
            <authorList>
                <person name="Raimo G."/>
                <person name="Masullo M."/>
                <person name="Parente A."/>
                <person name="Dello Russo A."/>
                <person name="Bocchini V."/>
            </authorList>
            <dbReference type="PubMed" id="1390884"/>
            <dbReference type="DOI" id="10.1016/0167-4781(92)90002-H"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-23 AND 590-604</scope>
        <scope>DIPHTHAMIDE AT HIS-603</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="X69297">
        <property type="protein sequence ID" value="CAA49157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
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    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
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        <property type="entry name" value="G_TR_1"/>
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    </dbReference>
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        <property type="entry name" value="G_TR_2"/>
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    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
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    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed" evidence="2">
        <location>
            <position position="1"/>
        </location>
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            <end position="736"/>
        </location>
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        <location>
            <begin position="18"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="93"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>GTP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="147"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>GTP</name>
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        </ligand>
    </feature>
    <feature type="modified residue" description="Diphthamide" evidence="2">
        <location>
            <position position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
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        <source>
            <dbReference type="PubMed" id="1390884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="736" mass="81820" checksum="06A96231E6EE83E6" modified="2007-01-23" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXA1</accession>
    <name>Q7LXA1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
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            <taxon>Sulfolobales</taxon>
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            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="SSO2092"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="183" mass="20505" checksum="9C3F2ACC252D94BE" modified="2011-05-31" version="1">MNRSFNIIISFKFIFWNMKSKPMNVIFDVLNEIHGFFGALWAGAALLNFLIKPQDKRQFERIGRFFLITSIITIITGIIIFAYIYLAPYQGNLFLVAAILRSSLDIRIRAFLNLIGGAFGLLAFGAGIVISNRIRLMVRVKEGDVTILELRNSVSNLSKISLVFLLISLAMMILAGSIAQVIT</sequence>
</entry>
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    <accession>Q7LXE6</accession>
    <name>Q7LXE6_SACS2</name>
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        <submittedName>
            <fullName evidence="2">dTDP-4-dehydrorhamnose reductase (RfbD-1)</fullName>
            <ecNumber evidence="2">1.1.1.133</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rfbD-1</name>
        <name evidence="2" type="ordered locus">SSO0832</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.133" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90233">
        <property type="entry name" value="H90233"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXE6"/>
    <dbReference type="FunCoup" id="Q7LXE6">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0832"/>
    <dbReference type="PaxDb" id="273057-SSO0832"/>
    <dbReference type="EnsemblBacteria" id="AAK41127">
        <property type="protein sequence ID" value="AAK41127"/>
        <property type="gene ID" value="SSO0832"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0832"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.847"/>
    <dbReference type="eggNOG" id="arCOG01367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045518_2_4_2"/>
    <dbReference type="InParanoid" id="Q7LXE6"/>
    <dbReference type="PhylomeDB" id="Q7LXE6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008831">
        <property type="term" value="F:dTDP-4-dehydrorhamnose reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05254">
        <property type="entry name" value="dTDP_HR_like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43242">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43242:SF1">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RmlD-like substrate binding" evidence="1">
        <location>
            <begin position="3"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="226" mass="25727" checksum="AB77A312E0B1B1F1" modified="2011-05-31" version="1">MEDFIIKTRPDVIINTAAMTDVDKCEVEREKAYKINAEAVKHMVRASRVVEAYFIHVSTDYVFDGTKGNYKEDDLPNPINYYGLTKLLGETFALSYDDSLVIRTSGVFRHKGFPVYVYKTLKEGKTVFAYKGYYSPISARKLASAIEELLALRKTGLLNVAGERISRYELALKIKEKFNLPGEVKEVDEVKGWTARRPFDSSLDYSKAKKILSVDFYSLDLEGMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXF2</accession>
    <name>Q7LXF2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0826</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99233">
        <property type="entry name" value="B99233"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923116.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXF2"/>
    <dbReference type="STRING" id="273057.SSO0826"/>
    <dbReference type="PaxDb" id="273057-SSO0826"/>
    <dbReference type="EnsemblBacteria" id="AAK41121">
        <property type="protein sequence ID" value="AAK41121"/>
        <property type="gene ID" value="SSO0826"/>
    </dbReference>
    <dbReference type="GeneID" id="1455088"/>
    <dbReference type="KEGG" id="sso:SSO0826"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.837"/>
    <dbReference type="eggNOG" id="arCOG01398">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2313921_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXF2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="99" mass="12152" checksum="AEB27655B6219538" modified="2011-05-31" version="1">MQIIYRLSKGSEIEKFLRYDYLFLKVLHKLFRNTKKKPSDDICKLNFYDILKVINNPYAEDRLRNFILAIASYFPFTLRKIILRRIYNFRVKMYNNLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXG0</accession>
    <name>Q7LXG0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Class I SAM-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0817</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99232">
        <property type="entry name" value="B99232"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXG0"/>
    <dbReference type="STRING" id="273057.SSO0817"/>
    <dbReference type="PaxDb" id="273057-SSO0817"/>
    <dbReference type="EnsemblBacteria" id="AAK41113">
        <property type="protein sequence ID" value="AAK41113"/>
        <property type="gene ID" value="SSO0817"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0817"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.829"/>
    <dbReference type="eggNOG" id="arCOG01773">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2257488_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXG0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="13076" checksum="A009EA693B892529" modified="2011-05-31" version="1">MYSYMPKDPIKEFYEQHYAKLELTAHMLRRIKLTEDAIEKFAKSKESILEIGCGTGENLSYYVNKFHFTNAYCVEIASFAEMEIREKGITPFILDVNVTEIPLEISSIDVLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXP2</accession>
    <name>Q7LXP2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH dehydrogenase subunit B (NuoB)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NuoB</name>
        <name evidence="7" type="ordered locus">SSO0665</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90214">
        <property type="entry name" value="B90214"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991208.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXP2"/>
    <dbReference type="SMR" id="Q7LXP2"/>
    <dbReference type="FunCoup" id="Q7LXP2">
        <property type="interactions" value="212"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0665"/>
    <dbReference type="PaxDb" id="273057-SSO0665"/>
    <dbReference type="EnsemblBacteria" id="AAK40969">
        <property type="protein sequence ID" value="AAK40969"/>
        <property type="gene ID" value="SSO0665"/>
    </dbReference>
    <dbReference type="GeneID" id="1454936"/>
    <dbReference type="KEGG" id="sso:SSO0665"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.666"/>
    <dbReference type="eggNOG" id="arCOG01554">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055737_7_3_2"/>
    <dbReference type="InParanoid" id="Q7LXP2"/>
    <dbReference type="PhylomeDB" id="Q7LXP2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12280:FF:000002">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006138">
        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01957">
        <property type="entry name" value="nuoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005012">
        <property type="entry name" value="PRK06411.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="6">
        <location>
            <begin position="49"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="170" mass="18823" checksum="269C0A6D2517891B" modified="2011-05-31" version="1">MTEQTILMGNLNEVAKKAAQWLINRKPIKSIIDWGISFSLWPPHFTTSCCGTEFGAFAAARFDAERYGMLPFSSARQSNILILEGTLSRKMGRAARIVYDQMPEPKYVIALGACILEGGIFWNSYNTVLPSDIGIPVDLYVPGCPIRPEAVARAVLMLQKKIRTQGALKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXX0</accession>
    <name>Q7LXX0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90197">
        <property type="entry name" value="H90197"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988379.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXX0"/>
    <dbReference type="PaxDb" id="273057-SSO0521"/>
    <dbReference type="EnsemblBacteria" id="AAK40839">
        <property type="protein sequence ID" value="AAK40839"/>
        <property type="gene ID" value="SSO0521"/>
    </dbReference>
    <dbReference type="GeneID" id="1454806"/>
    <dbReference type="KEGG" id="sso:SSO0521"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.515"/>
    <dbReference type="eggNOG" id="arCOG07733">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2366353_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXX0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="104" mass="11535" checksum="1C0E2E3D28DF66B6" modified="2011-05-31" version="1">MKRSIVLLSMVSIVLIVLGGYLFAIDKPTVVYPEIKGQIITETGVPNAYWWGYPAIIVTPQELAQLKQLAPHIVPPPWYATLWPIPLVGGIVGLIYSVVRLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TX4</accession>
    <name>Q97TX4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Arginine deiminase, hypothetical</fullName>
            <ecNumber evidence="1">3.5.3.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1148</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.3.6" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90267">
        <property type="entry name" value="H90267"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923212.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TX4"/>
    <dbReference type="STRING" id="273057.SSO1148"/>
    <dbReference type="PaxDb" id="273057-SSO1148"/>
    <dbReference type="EnsemblBacteria" id="AAK41399">
        <property type="protein sequence ID" value="AAK41399"/>
        <property type="gene ID" value="SSO1148"/>
    </dbReference>
    <dbReference type="GeneID" id="7806693"/>
    <dbReference type="KEGG" id="sso:SSO1148"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1140"/>
    <dbReference type="eggNOG" id="arCOG03108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052662_0_1_2"/>
    <dbReference type="InParanoid" id="Q97TX4"/>
    <dbReference type="PhylomeDB" id="Q97TX4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016990">
        <property type="term" value="F:arginine deiminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019546">
        <property type="term" value="P:L-arginine deiminase pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47271">
        <property type="entry name" value="ARGININE DEIMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47271:SF2">
        <property type="entry name" value="ARGININE DEIMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02274">
        <property type="entry name" value="ADI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="341" mass="39448" checksum="A1DC226C650607F2" modified="2001-10-01" version="1">MIAEWGKLRAVGMHKPGLEVMLGLLDPSSFLYERQFNWSKARREHDNLRRVLKEEGVKIYRLRQTIVNKIRRDDRFREKVIEKVGVNNDDPEFLVELLILRPILGYEKRDKGTHLEVKLTDPLSNLYFMRDQQITTEKGIIMGRMATHQREKEVEVMKLFWEALNIKYKEIGRGKFEGGDFFPMGDFNLVGVGVRTDLEGVSELFNILNGGEIGIIHWNREEFIHLDTFFNVGSSSSVVSVRSFMEESRVAVYYDRKIIRETTLYDYVVKEKGFNLIEVSVDEAKKYVTNFLTIDDGKIVSPKISANRKLEKEYDVIEVEVSNLTGGAGGIHCMTAVIRRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TX8</accession>
    <name>DPHB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Diphthine synthase</fullName>
            <ecNumber evidence="1">2.1.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Diphthamide biosynthesis methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dphB</name>
        <name type="ordered locus">SSO0953</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 3 S-adenosyl-L-methionine = diphthine-[translation elongation factor 2] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36415"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="2.1.1.98"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the diphthine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.98" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90246">
        <property type="entry name" value="D90246"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992392.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TX8"/>
    <dbReference type="SMR" id="Q97TX8"/>
    <dbReference type="FunCoup" id="Q97TX8">
        <property type="interactions" value="246"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0953"/>
    <dbReference type="PaxDb" id="273057-SSO0953"/>
    <dbReference type="EnsemblBacteria" id="AAK41227">
        <property type="protein sequence ID" value="AAK41227"/>
        <property type="gene ID" value="SSO0953"/>
    </dbReference>
    <dbReference type="GeneID" id="1455194"/>
    <dbReference type="KEGG" id="sso:SSO0953"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.949"/>
    <dbReference type="eggNOG" id="arCOG04161">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066040_0_0_2"/>
    <dbReference type="InParanoid" id="Q97TX8"/>
    <dbReference type="PhylomeDB" id="Q97TX8"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004164">
        <property type="term" value="F:diphthine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156128" description="Diphthine synthase">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
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        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <begin position="117"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01084"/>
        </source>
    </evidence>
    <sequence length="257" mass="28834" checksum="218BE19B593BF01B" modified="2001-10-01" version="1">MSILSLVGLGISKKFITDSAIETLSNSDIIIFDRYTSRSCDINVDVLRRLVKGEREFIEADRSLLENNSKAIIDYLDKGYNVSIASIGDALIATTHVSLLIEAKHRGHEVKVIPGISVHCYLISKSLLSSYKFGKSVTVTFPYNDFIDPTPYNVIKDNKERGLHTILYLDLKNEKAMTANEALQILLRLEERHKKSVLSKSDIIIVGARLGCDDERIIALKVEEATSFDFGNTPHIIIIPGNLHYMEADAIKWILRS</sequence>
</entry>
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    <accession>Q97U26</accession>
    <name>Q97U26_SACS2</name>
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        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="7" type="ordered locus">SSO3199</name>
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        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
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            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    <dbReference type="EnsemblBacteria" id="AAK43296">
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    </dbReference>
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        <property type="entry name" value="P_starv_induced_PsiE-like"/>
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        <property type="entry name" value="PsiE"/>
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    </dbReference>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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            <end position="36"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="128" mass="14356" checksum="AFB4B501F9BAC8E4" modified="2001-10-01" version="1">MARISDKDLIKFVGYIIRIILFFGIGVQIIITVYDIVTSIFSLNLLDLVNATITGPLLILVLIELYIAVNSYLTGKERSIINVIDAGISFFVRELILELFSHNYTITNIFIIAGIVGILSFSRFIANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U43</accession>
    <name>Q97U43_SACS2</name>
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        <submittedName>
            <fullName evidence="7">Phosphate transporter related protein</fullName>
        </submittedName>
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        <name evidence="7" type="ordered locus">SSO3180</name>
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    </organism>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
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                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
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                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <dbReference type="EnsemblBacteria" id="AAK43279">
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        <property type="gene ID" value="SSO3180"/>
    </dbReference>
    <dbReference type="GeneID" id="1453198"/>
    <dbReference type="KEGG" id="sso:SSO3180"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3284"/>
    <dbReference type="eggNOG" id="arCOG02682">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_14_2"/>
    <dbReference type="InParanoid" id="Q97U43"/>
    <dbReference type="PhylomeDB" id="Q97U43"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
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    </dbReference>
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        <property type="entry name" value="MFS_dom"/>
    </dbReference>
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        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
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        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
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        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
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        <location>
            <begin position="25"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAK43279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="471" mass="50732" checksum="49BBD2AA540D1E47" modified="2001-10-01" version="1">MDKGISKTPFGSIDSLKLTFNHIKVWYTSGMGFFTDAYDLFIISAILDVLLQLHDPNFPLNSVTEGLLASSALWAAIIGQLVFGFLGDKIGRKAIYGVEAILMTVGALLSALSPNIYWLIIFRSIMGLGIGGDYPISATIMSEYANVKDRGKLIALVFANQGLGSLAAVSVGIGSVLAFPLDISWRVMAAIGAIPAATVIYLRRKTPETPRYSMLVKGNVQEAKKAAEFLGAKIEEKRAYSKPLSLSEFLSKYWLILIGTAVPWFILDIAFYGTGIYSGAITQLILGKPTSIANLILEQGLPYMVGFFGYFTAVALMDKLGRKIIQLQGFILMTIIYAVVSSFLIVSGTKVVGLTIPAGIGFLIYALSFFFIDFGPNTTTFILPAEAYPTRARTTGHGISAASGKLGAAITTYLFPSLLASMGIKNILLMLSALSLVGAIVTIIAVKETKGKSLEEISKEEVIVQEEQFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U99</accession>
    <name>Q97U99_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Metabolite transport protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO3120</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90495">
        <property type="entry name" value="G90495"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U99"/>
    <dbReference type="STRING" id="273057.SSO3120"/>
    <dbReference type="PaxDb" id="273057-SSO3120"/>
    <dbReference type="EnsemblBacteria" id="AAK43222">
        <property type="protein sequence ID" value="AAK43222"/>
        <property type="gene ID" value="SSO3120"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3120"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3227"/>
    <dbReference type="eggNOG" id="arCOG02686">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_2"/>
    <dbReference type="InParanoid" id="Q97U99"/>
    <dbReference type="PhylomeDB" id="Q97U99"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="40"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="483" mass="53321" checksum="97D3440613D060AC" modified="2001-10-01" version="1">MYDYSDGESMSQYSKEEIDRGIKKIYDTVLNTKNITSRYIVILALASLWLDAYDFASMTFGTASLKSTFPSVPSVLISLAIGAVQLGAIIGAVVGGWLNDRIGRRNMFILNMILFTGMAILGGLSTNILELSIFRGLLGFALGADTATGFAYIFEYLEKKQRLFWSNLWQIQWYIMYEVTIALIVVPFFFTVHSLTSPLLWRIIMIVGGVLALVILLLRGRIPESVLWLAYQGRLATAKKILKQTYGIDLPEVPDVDLQLRAPARGVRSAFSIFRASKWRELVYSFNGNFEQGFIFYTFGFYVPYILLALKLAGPLASIVASAFLYAGGVIGGYLTAWLTPKIGTKSQYVIGAIGEGISVGLIALTYIYHLPLIYFVVFSFLFYFFHVIGPASQGMTSINAFFGAKERGTAAGWGYFWVKLAAFIGLLIGIVGIAYNPVSLTLGLAIYGVLTGIVGLIIGYDTRTYKLADVEELGENPQESVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UB4</accession>
    <name>Q97UB4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90493">
        <property type="entry name" value="H90493"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990924.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UB4"/>
    <dbReference type="PaxDb" id="273057-SSO3104"/>
    <dbReference type="EnsemblBacteria" id="AAK43207">
        <property type="protein sequence ID" value="AAK43207"/>
        <property type="gene ID" value="SSO3104"/>
    </dbReference>
    <dbReference type="GeneID" id="1453126"/>
    <dbReference type="KEGG" id="sso:SSO3104"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3211"/>
    <dbReference type="eggNOG" id="arCOG06029">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116131_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UB4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="177" mass="19157" checksum="124E1921796DC8CB" modified="2001-10-01" version="1">MNKLLLLGVLLSTILVGGVVIGEEISGSLGTISYNVTSPTIQTTLASFNLGNLTAGQKGTLTENATLTVSANGTYTIKLKEDVLGDVFSEFNVTIHIANYTFTLSLHGTEHYTLYLSKGTYTVNIKISYVVSQNPKAKSVSNIPFLMVKYGGEQQAQQTQTAEHGKKHHDDDNEQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UF1</accession>
    <name>Q97UF1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90489">
        <property type="entry name" value="C90489"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990872.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UF1"/>
    <dbReference type="FunCoup" id="Q97UF1">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3071"/>
    <dbReference type="PaxDb" id="273057-SSO3071"/>
    <dbReference type="EnsemblBacteria" id="AAK43170">
        <property type="protein sequence ID" value="AAK43170"/>
        <property type="gene ID" value="SSO3071"/>
    </dbReference>
    <dbReference type="GeneID" id="1453089"/>
    <dbReference type="KEGG" id="sso:SSO3071"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3170"/>
    <dbReference type="eggNOG" id="arCOG02462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030671_3_0_2"/>
    <dbReference type="InParanoid" id="Q97UF1"/>
    <dbReference type="PhylomeDB" id="Q97UF1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.50:FF:000005">
        <property type="entry name" value="Cyclase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31118">
        <property type="entry name" value="CYCLASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31118:SF12">
        <property type="entry name" value="CYCLASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="243" mass="27787" checksum="475CB2A0FB567EAA" modified="2001-10-01" version="1">MIEELFDQMSKGNLLLIDMTYDMFNGMQTYIGDPPFKHEYFRKGNKYGEVTLSTVYMGLHSGTHIDLPLHFVPNGESVEKFNIIQFIGYGNVLDLSYKKLGEPISSNDLKKFDDRIKENYVVMLYTGFSATRGKEDFLYNWPYLDSNGADYLVSKKIRAVGIESMSIAGWAGKEYPPRTNWDEVVYVHHKLLSNDIIILESVNNMKKVLEECKNGEALFFYAPLKIVGAEGGPTRLFAICEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UP3</accession>
    <name>Q97UP3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="4">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ilvB-5</name>
        <name evidence="11" type="ordered locus">SSO2962</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="4"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90476">
        <property type="entry name" value="B90476"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924062.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UP3"/>
    <dbReference type="FunCoup" id="Q97UP3">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2962"/>
    <dbReference type="PaxDb" id="273057-SSO2962"/>
    <dbReference type="EnsemblBacteria" id="AAK43065">
        <property type="protein sequence ID" value="AAK43065"/>
        <property type="gene ID" value="SSO2962"/>
    </dbReference>
    <dbReference type="GeneID" id="1452984"/>
    <dbReference type="KEGG" id="sso:SSO2962"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3051"/>
    <dbReference type="eggNOG" id="arCOG01999">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052737">
        <property type="term" value="F:pyruvate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02014">
        <property type="entry name" value="TPP_POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07039">
        <property type="entry name" value="TPP_PYR_POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047211">
        <property type="entry name" value="POXB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047212">
        <property type="entry name" value="TPP_POXB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047210">
        <property type="entry name" value="TPP_PYR_POXB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005040">
        <property type="entry name" value="PRK06457.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42981">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE [UBIQUINONE]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42981:SF2">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE [UBIQUINONE]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="10">
        <location>
            <begin position="4"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="8">
        <location>
            <begin position="187"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="9">
        <location>
            <begin position="373"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK43065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="552" mass="61094" checksum="EC1AFAF391A6A09F" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UP8</accession>
    <name>Q97UP8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ORF in partial transposon ISC774</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO11553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="Q97UP8"/>
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    <dbReference type="EnsemblBacteria" id="AAK43060">
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    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_2230495_0_0_2"/>
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    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="84" mass="9716" checksum="4AF66DD810C5B0DA" modified="2001-10-01" version="1">MHDGLSVCNAFDWFNVGHKRVTLGDYTEQGFRKLKHRVSSMDFACNSNKLMSWLSTFFLIYNLLYIQVYLVDKGVMINANISTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UQ7</accession>
    <name>Q97UQ7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3782 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
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        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
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            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90474">
        <property type="entry name" value="B90474"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924048.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UQ7"/>
    <dbReference type="PaxDb" id="273057-SSO2946"/>
    <dbReference type="EnsemblBacteria" id="AAK43049">
        <property type="protein sequence ID" value="AAK43049"/>
        <property type="gene ID" value="SSO2946"/>
    </dbReference>
    <dbReference type="GeneID" id="1452968"/>
    <dbReference type="KEGG" id="sso:SSO2946"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3033"/>
    <dbReference type="eggNOG" id="arCOG01422">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064028_1_0_2"/>
    <dbReference type="InParanoid" id="Q97UQ7"/>
    <dbReference type="PhylomeDB" id="Q97UQ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024271">
        <property type="entry name" value="DUF3782"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012431">
        <property type="entry name" value="PDDEXK_10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34314">
        <property type="entry name" value="CRENARCHAEAL PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34314:SF7">
        <property type="entry name" value="DUF3782 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12644">
        <property type="entry name" value="DUF3782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07788">
        <property type="entry name" value="PDDEXK_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="41"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="27925" checksum="2769F018D9C47875" modified="2001-10-01" version="1">MSLEEEIKKVLLNNPSILVDVLTAKPEIVYQVLARLTPWQSLATKQDVERLDKEIEEVKKTMATKQDVERLDKEIEEVKKTMATKQDVERLDKEIEEVKKTMATKEDLKRLETIITGLGARWGILNEDSFRQGISEILSNSGWKISREVLYDRDGYVYDEPSDVEYDIVVKDSSVILVEITSSIRRGDLPTIKKKKEFYEKVKNIKVNLVYVITPFIHDRYPERIKAMAKDMGIEIIYPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UX1</accession>
    <name>Q97UX1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2870</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90465">
        <property type="entry name" value="B90465"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989830.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UX1"/>
    <dbReference type="STRING" id="273057.SSO2870"/>
    <dbReference type="PaxDb" id="273057-SSO2870"/>
    <dbReference type="EnsemblBacteria" id="AAK42977">
        <property type="protein sequence ID" value="AAK42977"/>
        <property type="gene ID" value="SSO2870"/>
    </dbReference>
    <dbReference type="GeneID" id="7807700"/>
    <dbReference type="KEGG" id="sso:SSO2870"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2956"/>
    <dbReference type="eggNOG" id="arCOG00826">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_36_1_2"/>
    <dbReference type="InParanoid" id="Q97UX1"/>
    <dbReference type="PhylomeDB" id="Q97UX1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050832">
        <property type="entry name" value="Bact_Acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43877:SF1">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43877">
        <property type="entry name" value="AMINOALKYLPHOSPHONATE N-ACETYLTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="154" mass="18139" checksum="FD2220E4E3E076D2" modified="2001-10-01" version="1">MVTIRRATKEDVKTLIDFFSRMYRLNSEFDPLLLTPENLEERINKVVEKSLEDQNEMIVVAEDQGRIVGAARVLIIDRIFYTPEKEALIREFYVHPSYRRQGVGNEIVNFIISELKERGIEILGAEFPSRNLIAISFYRKMGFREIYCEFVKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UX7</accession>
    <name>Q97UX7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GPR1/FUN34/yaaH family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2864</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90464">
        <property type="entry name" value="D90464"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UX7"/>
    <dbReference type="FunCoup" id="Q97UX7">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2864"/>
    <dbReference type="PaxDb" id="273057-SSO2864"/>
    <dbReference type="EnsemblBacteria" id="AAK42971">
        <property type="protein sequence ID" value="AAK42971"/>
        <property type="gene ID" value="SSO2864"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2864"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2950"/>
    <dbReference type="eggNOG" id="arCOG03176">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051062_3_0_2"/>
    <dbReference type="InParanoid" id="Q97UX7"/>
    <dbReference type="PhylomeDB" id="Q97UX7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015360">
        <property type="term" value="F:acetate:proton symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035433">
        <property type="term" value="P:acetate transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071422">
        <property type="term" value="P:succinate transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047622">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047623">
        <property type="entry name" value="SatP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30178">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YAAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30178:SF3">
        <property type="entry name" value="SUCCINATE-ACETATE_PROTON SYMPORTER SATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01114">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="20361" checksum="0E45B3F7FEB95797" modified="2001-10-01" version="1">MLYKDVMTEQKRANPAPLGLSGFALTTLVLSTFNAGLITQGASVVLGLAAFYGGLAQLLAGILEWRAGNTFGYTAFFTYGAFWEWYFLTAGGFFGGVTPQAIGLVLIAFGIFTLAMWFGTFKANLGLFMTFLLLWITFFLLGVGAMIGNVGLSHAGGYVGILTAIAAWYTGLAIVVAESLGKSPPVGRPIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UZ7</accession>
    <name>Q97UZ7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Partial transposase ISC1250</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2842</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90461">
        <property type="entry name" value="G90461"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UZ7"/>
    <dbReference type="PaxDb" id="273057-SSO2842"/>
    <dbReference type="EnsemblBacteria" id="AAK42950">
        <property type="protein sequence ID" value="AAK42950"/>
        <property type="gene ID" value="SSO2842"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2842"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2926"/>
    <dbReference type="eggNOG" id="arCOG04918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1599048_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UZ7"/>
    <dbReference type="PhylomeDB" id="Q97UZ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217:SF7">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="189" mass="21692" checksum="709CA486E7240A13" modified="2001-10-01" version="1">MLVPNILSEDALLNKSVKEIEKIALEEADKTSPKHVRGKKIRRKGYAKYRFLKGFKIMLKGREDIYKLERISVELPVLYSDKGRVKTQVEERLLREEKVFKALMTVYSVLGGKLNAKLWMLEIQVSGDFKYVIVDGKYVKLKGGRGSFSAIGVTEEGKRAVLDITLSVEKNAMGYWRLLVRVWKKYSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V50</accession>
    <name>Q97V50_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Third ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90454">
        <property type="entry name" value="H90454"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V50"/>
    <dbReference type="STRING" id="273057.SSO2784"/>
    <dbReference type="PaxDb" id="273057-SSO2784"/>
    <dbReference type="EnsemblBacteria" id="AAK42895">
        <property type="protein sequence ID" value="AAK42895"/>
        <property type="gene ID" value="SSO2784"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2784"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2867"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074024_1_0_2"/>
    <dbReference type="InParanoid" id="Q97V50"/>
    <dbReference type="PhylomeDB" id="Q97V50"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="203" mass="24132" checksum="F699F469FAA9AC66" modified="2001-10-01" version="1">MTKDEIVKILIEQVVAMGFRIKLIALDAGFYTVEVIKFISQFNYIIGVPVDDVKIYEEFDGEYVTNSKRHSKDEQVKFRLIVYRREKIKRKRRRLFTSPGLQISIYPRIRSWSNKVRNPIETSYRSVKSFLPFTCSTKFIFRMLIFLLDVLVYSLYTTLKDNVKMRTFKSLISKIIENISEIEIYLNKLEETLTNTIGLFLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VA0</accession>
    <name>Q97VA0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO11020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90448">
        <property type="entry name" value="F90448"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990282.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VA0"/>
    <dbReference type="STRING" id="273057.SSO11020"/>
    <dbReference type="PaxDb" id="273057-SSO11020"/>
    <dbReference type="EnsemblBacteria" id="AAK42845">
        <property type="protein sequence ID" value="AAK42845"/>
        <property type="gene ID" value="SSO11020"/>
    </dbReference>
    <dbReference type="GeneID" id="1452764"/>
    <dbReference type="KEGG" id="sso:SSO11020"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2814"/>
    <dbReference type="eggNOG" id="arCOG08517">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2379564_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VA0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="94" mass="10459" checksum="8FAAC9565D233288" modified="2001-10-01" version="1">MDYSAILGTLTKDKRECQSFLSICNNGKFVFVAICDTQTKVYCGEVNENSIIFGEKGNNNSVTYEIPISDNNIKVIKVSDSIYAYNVNESLLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VD0</accession>
    <name>Q97VD0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2702</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90444">
        <property type="entry name" value="G90444"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988627.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VD0"/>
    <dbReference type="PaxDb" id="273057-SSO2702"/>
    <dbReference type="EnsemblBacteria" id="AAK42814">
        <property type="protein sequence ID" value="AAK42814"/>
        <property type="gene ID" value="SSO2702"/>
    </dbReference>
    <dbReference type="GeneID" id="1452733"/>
    <dbReference type="KEGG" id="sso:SSO2702"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2782"/>
    <dbReference type="eggNOG" id="arCOG06006">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_798378_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VD0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="347" mass="39742" checksum="1E4044BB0F5EF0C7" modified="2001-10-01" version="1">MGLNRKLLVFGFSFSSIGGPLALVGQFLENVNPFEVVLSVLIFSPITFLVYYSMNKVWDKGGLYDYVVKFTPKLSSYFLYFWFFSYFLYLSYTVDYIVYYILNLDGFTAILLTIGIAGSIAMITLLDRELEFLFVSAIIQMLFILPINWHLKFMNPYELSFTNILSTSLLYICITLTPFIGNGSKEGINSVILAYLITGAILFLDSFFNIPKIIYLISSFSTFSLIVVEFYSLKSVLSKFSIKSRKYLVLGFLGFTLTSLINPYMYYIYTIVPSLTALYISLIIFFISMTFSIRNELKLLGFISLGLIAYGLYSSLQLSSYYLLLEQIIIIIIIGVIPLLKNRLFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VF0</accession>
    <name>Q97VF0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2677</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90442">
        <property type="entry name" value="C90442"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VF0"/>
    <dbReference type="PaxDb" id="273057-SSO2677"/>
    <dbReference type="EnsemblBacteria" id="AAK42794">
        <property type="protein sequence ID" value="AAK42794"/>
        <property type="gene ID" value="SSO2677"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2677"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2758"/>
    <dbReference type="eggNOG" id="arCOG05362">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137177_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VF0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="164" mass="18994" checksum="A26F5535EE69292A" modified="2001-10-01" version="1">MTNSSCIFHLLYIENKKLYVTCQKPFIQRNYIYSMNPFKKEKTYPKQVDLNKVVNEFVSYLNIQKWKVQQKVEGNKAIVQAQKGGILRDIIAADRALTFTFENTTQGLKVTAGIGSWKKNLAITALETLFLSELFLVVDVPEMLWNEHVESELMKKLDEIIQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VM5</accession>
    <name>Q97VM5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Serine-pyruvate aminotransferase (AgxT)</fullName>
            <ecNumber evidence="9">2.6.1.51</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">agxT</name>
        <name evidence="9" type="ordered locus">SSO2597</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="11 12 13" key="2">
        <citation type="journal article" date="2012" name="Acta Crystallogr. D" volume="68" first="763" last="772">
            <title>Crystal structure and substrate specificity of the thermophilic serine:pyruvate aminotransferase from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Sayer C."/>
                <person name="Bommer M."/>
                <person name="Isupov M."/>
                <person name="Ward J."/>
                <person name="Littlechild J."/>
            </authorList>
            <dbReference type="PubMed" id="22751661"/>
            <dbReference type="DOI" id="10.1107/s0907444912011274"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) IN COMPLEX WITH CA(2+) AND PYRIDOXAL 5'-PHOSPHATE</scope>
        <scope>PYRIDOXAL PHOSPHATE AT LYS-189</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.51" evidence="9"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90432">
        <property type="entry name" value="H90432"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989311.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3ZRP">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.75 A"/>
        <property type="chains" value="A/B=1-384"/>
    </dbReference>
    <dbReference type="PDB" id="3ZRQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B=1-384"/>
    </dbReference>
    <dbReference type="PDB" id="3ZRR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.99 A"/>
        <property type="chains" value="A/B=1-384"/>
    </dbReference>
    <dbReference type="PDBsum" id="3ZRP"/>
    <dbReference type="PDBsum" id="3ZRQ"/>
    <dbReference type="PDBsum" id="3ZRR"/>
    <dbReference type="AlphaFoldDB" id="Q97VM5"/>
    <dbReference type="SMR" id="Q97VM5"/>
    <dbReference type="FunCoup" id="Q97VM5">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2597"/>
    <dbReference type="PaxDb" id="273057-SSO2597"/>
    <dbReference type="EnsemblBacteria" id="AAK42719">
        <property type="protein sequence ID" value="AAK42719"/>
        <property type="gene ID" value="SSO2597"/>
    </dbReference>
    <dbReference type="GeneID" id="1454046"/>
    <dbReference type="KEGG" id="sso:SSO2597"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2678"/>
    <dbReference type="eggNOG" id="arCOG00082">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027686_5_2_2"/>
    <dbReference type="InParanoid" id="Q97VM5"/>
    <dbReference type="PhylomeDB" id="Q97VM5"/>
    <dbReference type="BRENDA" id="2.6.1.51">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97VM5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004760">
        <property type="term" value="F:L-serine-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019265">
        <property type="term" value="P:glycine biosynthetic process, by transamination of glyoxylate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000191">
        <property type="entry name" value="Serine-pyruvate aminotransferase (AgxT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152:SF24">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152">
        <property type="entry name" value="AMINOTRANSFERASE CLASS V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="11 12 13" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="8">
        <location>
            <begin position="29"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine (covalent)" evidence="11">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="11">
        <source>
            <dbReference type="PDB" id="3ZRP"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="12">
        <source>
            <dbReference type="PDB" id="3ZRQ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="13">
        <source>
            <dbReference type="PDB" id="3ZRR"/>
        </source>
    </evidence>
    <sequence length="384" mass="41977" checksum="032540C7080B6160" modified="2001-10-01" version="1">MDKLLLHVGPTTIKEDVLVAGLENNVGFTSKEFVEALAYSLKGLRYVMGASKNYQPLIIPGGGTSAMESVTSLLKPNDKILVVSNGVFGDRWEQIFKRYPVNVKVLRPSPGDYVKPGEVEEEVRKSEYKLVALTHVETSTGVREPVKDVINKIRKYVELIVVDGVSSVGAEEVKAEEWNVDVYLTASQKALGSAAGLGLLLLSPKALSILDSQNSIAGYYLDLRNWLPVMRGAEEGKAAYFATPPVHVILQLAEAFRLIEKEGIENRIKRHTMVASAIRAGLEALGLEIVARRPESYSNTVTGVILKVADPQKVLAGTVNEGVEFAPGVHPAFKYFRIGHMGWVTPNDAIIAISVIERTLRKLGEPIRFGEGVKAVEEVLFSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VR5</accession>
    <name>Q97VR5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90427">
        <property type="entry name" value="E90427"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923916.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VR5"/>
    <dbReference type="STRING" id="273057.SSO2547"/>
    <dbReference type="PaxDb" id="273057-SSO2547"/>
    <dbReference type="EnsemblBacteria" id="AAK42676">
        <property type="protein sequence ID" value="AAK42676"/>
        <property type="gene ID" value="SSO2547"/>
    </dbReference>
    <dbReference type="GeneID" id="1454003"/>
    <dbReference type="KEGG" id="sso:SSO2547"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2628"/>
    <dbReference type="eggNOG" id="arCOG07325">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_357772_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VR5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="690"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="742"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="782" mass="88601" checksum="93172185CC0FE85A" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VX9</accession>
    <name>Q97VX9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2474</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90419">
        <property type="entry name" value="D90419"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923879.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VX9"/>
    <dbReference type="STRING" id="273057.SSO2474"/>
    <dbReference type="PaxDb" id="273057-SSO2474"/>
    <dbReference type="EnsemblBacteria" id="AAK42611">
        <property type="protein sequence ID" value="AAK42611"/>
        <property type="gene ID" value="SSO2474"/>
    </dbReference>
    <dbReference type="GeneID" id="84057809"/>
    <dbReference type="KEGG" id="sso:SSO2474"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2551"/>
    <dbReference type="eggNOG" id="arCOG02242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150444_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VX9"/>
    <dbReference type="PhylomeDB" id="Q97VX9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052362">
        <property type="entry name" value="HTH-GbsR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38465">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR MJ1563-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38465:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR MJ1563-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="4">
        <location>
            <begin position="25"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="131" mass="14981" checksum="DDC7D125AAF2F9AF" modified="2001-10-01" version="1">MQGKTEINMPDGRVADIFNVIKFLYGLSDRDIEILKLLLKSQNPLTMEEISSKLNITKSVVNKSILNLEKKNIIIKEKIETSKKGRRAYTYKVDSNYLNKKLLVDLDQLMKDLKVKIANVIGVEIEKTANI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VY4</accession>
    <name>Q97VY4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter, ATP binding protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2469</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90418">
        <property type="entry name" value="G90418"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VY4"/>
    <dbReference type="FunCoup" id="Q97VY4">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2469"/>
    <dbReference type="PaxDb" id="273057-SSO2469"/>
    <dbReference type="EnsemblBacteria" id="AAK42606">
        <property type="protein sequence ID" value="AAK42606"/>
        <property type="gene ID" value="SSO2469"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2469"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2546"/>
    <dbReference type="eggNOG" id="arCOG00193">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="InParanoid" id="Q97VY4"/>
    <dbReference type="PhylomeDB" id="Q97VY4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050166">
        <property type="entry name" value="ABC_transporter_ATP-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42788:SF13">
        <property type="entry name" value="ALIPHATIC SULFONATES IMPORT ATP-BINDING PROTEIN SSUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42788">
        <property type="entry name" value="TAURINE IMPORT ATP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="238" mass="26683" checksum="6E1A5525D8D05C1E" modified="2001-10-01" version="1">MFENINMYTKDRELIAIVGPSGIGKSTLLRVLGGFVKPLKGEIRLLGKKVTSPTPKIALIHQSIATFPWLTALENVKLGLKYRKLPKEEEDRLAKHMLEVVGLQGFEDFYPKQMSGGMRQRIAIARALAANPLVLLMDEPFSHLDELTAEGLRQEVHSMLFNESTTLHSVVLVSHNLSEVVELADRVYVLNGRPATVIGEVEIKLERPRNPKDESFQEYLDILYALLTPVKKGGNNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W15</accession>
    <name>Q97W15_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MobA-like NTP transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2432</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2008-05" db="PDB data bank">
            <title>Crystal structure of the Q97W15_SULSO protein from Sulfolobus solfataricus. NESG target SsR125.</title>
            <authorList>
                <person name="Vorobiev S.M."/>
                <person name="Chen Y."/>
                <person name="Seetharaman J."/>
                <person name="Lee D."/>
                <person name="Foote R.E."/>
                <person name="Maglaqui M."/>
                <person name="Janjua H."/>
                <person name="Xiao R."/>
                <person name="Acton T.B."/>
                <person name="Montelione G.T."/>
                <person name="Tong L."/>
                <person name="Hunt J.F."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS)</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90414">
        <property type="entry name" value="H90414"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989391.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3D5N">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B/D/E/F/G/H/I=1-189"/>
    </dbReference>
    <dbReference type="PDBsum" id="3D5N"/>
    <dbReference type="AlphaFoldDB" id="Q97W15"/>
    <dbReference type="SMR" id="Q97W15"/>
    <dbReference type="STRING" id="273057.SSO2432"/>
    <dbReference type="PaxDb" id="273057-SSO2432"/>
    <dbReference type="EnsemblBacteria" id="AAK42575">
        <property type="protein sequence ID" value="AAK42575"/>
        <property type="gene ID" value="SSO2432"/>
    </dbReference>
    <dbReference type="GeneID" id="1453902"/>
    <dbReference type="KEGG" id="sso:SSO2432"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2511"/>
    <dbReference type="eggNOG" id="arCOG01873">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061980_1_1_2"/>
    <dbReference type="InParanoid" id="Q97W15"/>
    <dbReference type="PhylomeDB" id="Q97W15"/>
    <dbReference type="EvolutionaryTrace" id="Q97W15"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061602">
        <property type="term" value="F:molybdenum cofactor cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902760">
        <property type="term" value="P:Mo(VI)-molybdopterin cytosine dinucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04182">
        <property type="entry name" value="GT_2_like_f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43777">
        <property type="entry name" value="MOLYBDENUM COFACTOR CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43777:SF1">
        <property type="entry name" value="MOLYBDENUM COFACTOR CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MobA-like NTP transferase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="116"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3D5N"/>
        </source>
    </evidence>
    <sequence length="189" mass="21376" checksum="E881A0B5F83347AA" modified="2001-10-01" version="1">MNIGVIILAAGEGKRFGGDKLLAKIDNTPIIMRTIRIYGDLEKIIIVGKYVNEMLPLLMDQIVIYNPFWNEGISTSLKLGLRFFKDYDAVLVALGDMPFVTKEDVNKIINTFKPNCKAVIPTHKGERGNPVLISKSLFNEIEKLRGDVGARVILNKIKIEELCFIECSEGVLIDIDKKEDLMRLRDFHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W37</accession>
    <name>Q97W37_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2406</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99412">
        <property type="entry name" value="B99412"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989427.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W37"/>
    <dbReference type="STRING" id="273057.SSO2406"/>
    <dbReference type="PaxDb" id="273057-SSO2406"/>
    <dbReference type="EnsemblBacteria" id="AAK42553">
        <property type="protein sequence ID" value="AAK42553"/>
        <property type="gene ID" value="SSO2406"/>
    </dbReference>
    <dbReference type="GeneID" id="1453880"/>
    <dbReference type="KEGG" id="sso:SSO2406"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2488"/>
    <dbReference type="eggNOG" id="arCOG00497">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070010_3_0_2"/>
    <dbReference type="InParanoid" id="Q97W37"/>
    <dbReference type="PhylomeDB" id="Q97W37"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42967:SF1">
        <property type="entry name" value="MBL FOLD METALLO-HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42967">
        <property type="entry name" value="METAL DEPENDENT HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13483">
        <property type="entry name" value="Lactamase_B_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="24378" checksum="5A698B3D4257FC1F" modified="2001-10-01" version="1">MVLKYFGHSCVLIDDRILIDPHDGGSIGLPKPDIKKVDLILVTHDHYDHNAYQIFEYKDVKINFYGSLTYSNYTIKGIKSYHDKYNGKLRGENSIYVLQRSDGKKIVHLGDLGHLLEENTYKELYGADVLLIPIGGVITINFKEALEIINKISPKIIIPIHYWIKGHYMPLDPPEEFLANLKYEIRRIDLKNNLIDENVEESKKVVYALSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W42</accession>
    <name>Q97W42_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transporter, ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2402</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90411">
        <property type="entry name" value="E90411"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W42"/>
    <dbReference type="FunCoup" id="Q97W42">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2402"/>
    <dbReference type="PaxDb" id="273057-SSO2402"/>
    <dbReference type="EnsemblBacteria" id="AAK42548">
        <property type="protein sequence ID" value="AAK42548"/>
        <property type="gene ID" value="SSO2402"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2402"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2483"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q97W42"/>
    <dbReference type="PhylomeDB" id="Q97W42"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43613">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43613:SF1">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="10"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="248" mass="28004" checksum="91278AD558FA9107" modified="2001-10-01" version="1">MSMTEKQFAVSVTNLRKRIGNKEILKGLSFDVESGEVFGIVGPNGAGKTTTLRILAGIIKNFNGDAKVFGLRPIEAKQNGYISYMPEDAFPYEKLTGYENLDFYAELYAKGDKELKEKYLKLGIEIANLGERIYDKTAEYSRGMKRRLIIARTLMVMPKLSVLDEPTSALDVESAVRIRNIILEMARRYNMTIILSSHNMLEVEYLCDRITLINDGRIVANGKPKEIIKSTNSKNLEEAFIKLVFGDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W43</accession>
    <name>Q97W43_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2401</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90411">
        <property type="entry name" value="D90411"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989435.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W43"/>
    <dbReference type="STRING" id="273057.SSO2401"/>
    <dbReference type="PaxDb" id="273057-SSO2401"/>
    <dbReference type="EnsemblBacteria" id="AAK42547">
        <property type="protein sequence ID" value="AAK42547"/>
        <property type="gene ID" value="SSO2401"/>
    </dbReference>
    <dbReference type="GeneID" id="1453874"/>
    <dbReference type="KEGG" id="sso:SSO2401"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2482"/>
    <dbReference type="eggNOG" id="arCOG04261">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1253581_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W43"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="227" mass="26704" checksum="881D2176AA451500" modified="2001-10-01" version="1">MIGTVKDRTLEAILRYGDKAKYILKAIIEITEENENRELGDFNYKRLVEKLEEWGINYDPRMILRALEKDFGIIETTYKSANQHWWKLIDIEQTKLAISGEIENGEEDAEISLIIIQYNSLNPKEVEKKLLFLLKKPKLLDLDKKNFMKFSFEILPLIVKLYYKASQHEETFEIAEKLKKIINLAKRISSRINNEKGDNKGFHKEEINEGENYDVNSLRLPHSENNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W81</accession>
    <name>Q97W81_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO10269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90406">
        <property type="entry name" value="D90406"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989498.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W81"/>
    <dbReference type="STRING" id="273057.SSO10269"/>
    <dbReference type="PaxDb" id="273057-SSO10269"/>
    <dbReference type="EnsemblBacteria" id="AAK42507">
        <property type="protein sequence ID" value="AAK42507"/>
        <property type="gene ID" value="SSO10269"/>
    </dbReference>
    <dbReference type="GeneID" id="1453834"/>
    <dbReference type="KEGG" id="sso:SSO10269"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2441"/>
    <dbReference type="eggNOG" id="arCOG05957">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2433664_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W81"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="96" mass="11378" checksum="F736D94B63A0D732" modified="2001-10-01" version="1">MSINKEKLGADKVIKNSLDYCDLYIIQKGDKVFLLYLFERRGYYYFKIMPEIVGKWEDCENVVYTAFGLFGFVSNQEELEQKIKEKIEVLMRNVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WA1</accession>
    <name>Q97WA1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alcohol dehydrogenase (Zn containing) (Adh-6)</fullName>
            <ecNumber evidence="5">1.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">adh-6</name>
        <name evidence="5" type="ordered locus">SSO2334</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.1" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90403">
        <property type="entry name" value="H90403"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WA1"/>
    <dbReference type="FunCoup" id="Q97WA1">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2334"/>
    <dbReference type="PaxDb" id="273057-SSO2334"/>
    <dbReference type="EnsemblBacteria" id="AAK42487">
        <property type="protein sequence ID" value="AAK42487"/>
        <property type="gene ID" value="SSO2334"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2334"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2421"/>
    <dbReference type="eggNOG" id="arCOG01458">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_4_2"/>
    <dbReference type="InParanoid" id="Q97WA1"/>
    <dbReference type="PhylomeDB" id="Q97WA1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030554">
        <property type="term" value="F:adenyl nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043168">
        <property type="term" value="F:anion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08264">
        <property type="entry name" value="Zn_ADH_like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF4">
        <property type="entry name" value="D-ARABINOSE 1-DEHYDROGENASE (NADP(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="4">
        <location>
            <begin position="16"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="338" mass="36931" checksum="92A1434D4EC21714" modified="2001-10-01" version="1">MQIRLTLMKALVFDKSGLENLKVKEVQEPQLGPHDVLIRVVKSGLNPIDYFVINAIPVTPMPHIPGAELAGVVEKVGDHVKGLNKGDKVVVYNRVFDGTCDLCLSGREMLCRNGGIMSVITNGGWSEYFSVPDKNIFKIPESMSWDVAASLPVAALTSYHSLKELEVGPNDIIVIFGASGNTGLFAVQLAKKFGATVIAVSRKNWLKEFGADYIVGYDDVVQKVKEITNGKMADVVINSLGSSMWENSLQVLGLDGQLAFFGTLTGSEVKVNLSSLYGRHIKVVGTTGGSRKELMELINLCKDCKVKVHKTYKLDEGVEAVKEIMNENREGRIMLQVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WB0</accession>
    <name>Q97WB0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90402">
        <property type="entry name" value="G90402"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992499.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WB0"/>
    <dbReference type="STRING" id="273057.SSO2325"/>
    <dbReference type="PaxDb" id="273057-SSO2325"/>
    <dbReference type="EnsemblBacteria" id="AAK42478">
        <property type="protein sequence ID" value="AAK42478"/>
        <property type="gene ID" value="SSO2325"/>
    </dbReference>
    <dbReference type="GeneID" id="1453805"/>
    <dbReference type="KEGG" id="sso:SSO2325"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2411"/>
    <dbReference type="eggNOG" id="arCOG07317">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_557385_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WB0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="313"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="466"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="489" mass="55976" checksum="827D0A52573EC6B1" modified="2001-10-01" version="1">MIRLDKRNFIPWISIVLLLCAYSIGTGLYITIQRVTYYPIFESKIVSVFLTIFSSSLITFYNYKKYVFLLPLSLLSFFSVSLTPLIISIFILYELKKVDRTVSIILLIINASMISWLILRLLLGINTYFSIPLMILEAGAPTVIPFIWFVGIILSAYKRNLSSKSQLFINPLIPFIVVLLISLIPYLPFINPYKFPETVDFKYYYSWLLAPTFSGWFFDSRPVYLMLLYALSLIFKPYTVAYYEFIFLSLLYTYSAYKLASAIDKSIASLSALLASVSPMLMTFLYSGLEANLFSISLMFISMSYLFKKEKLSLAILFSLLSMFSHIYAWAQLSTGITLYYLLKSIIHRSRPDNYTLTYLSFSIPFIAIGLFLILSGVFPLPMELLNYTQLIYQIAVVSWGSNNALLYFLLSSFGNRYVKEGVLNFVYSISVFGIIFVSSATNLIIDLPLFIPAAYTIRNVNRRSTSILLVLSLILWGIYMSINSVPML</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WD0</accession>
    <name>Q97WD0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cobalamin biosynthesis protein G homolog, putative (CbiG-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cbiG-like</name>
        <name evidence="4" type="ordered locus">SSO2297</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2008-09" db="PDB data bank">
            <title>Crystal structure of a putative cobalamin biosynthesis protein G homolog from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Bonanno J.B."/>
                <person name="Gilmore M."/>
                <person name="Bain K.T."/>
                <person name="Chang S."/>
                <person name="Romero R."/>
                <person name="Wasserman S."/>
                <person name="Sauder J.M."/>
                <person name="Burley S.K."/>
                <person name="Almo S.C."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 7-7; 10-126 AND 130-336</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90400">
        <property type="entry name" value="C90400"/>
    </dbReference>
    <dbReference type="PDB" id="3EEQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B=7-7, A/B=10-126, A/B=130-336"/>
    </dbReference>
    <dbReference type="PDBsum" id="3EEQ"/>
    <dbReference type="AlphaFoldDB" id="Q97WD0"/>
    <dbReference type="SMR" id="Q97WD0"/>
    <dbReference type="FunCoup" id="Q97WD0">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2297"/>
    <dbReference type="PaxDb" id="273057-SSO2297"/>
    <dbReference type="DNASU" id="1453785"/>
    <dbReference type="EnsemblBacteria" id="AAK42458">
        <property type="protein sequence ID" value="AAK42458"/>
        <property type="gene ID" value="SSO2297"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2297"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2390"/>
    <dbReference type="eggNOG" id="arCOG00651">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028397_0_2_2"/>
    <dbReference type="InParanoid" id="Q97WD0"/>
    <dbReference type="PhylomeDB" id="Q97WD0"/>
    <dbReference type="EvolutionaryTrace" id="Q97WD0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.180">
        <property type="entry name" value="CobE/GbiG C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048775">
        <property type="entry name" value="CbiG-like_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052553">
        <property type="entry name" value="CbiG_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021744">
        <property type="entry name" value="CbiG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002750">
        <property type="entry name" value="CobE/GbiG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036518">
        <property type="entry name" value="CobE/GbiG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038029">
        <property type="entry name" value="GbiG_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004467">
        <property type="entry name" value="PRK05788.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37477">
        <property type="entry name" value="COBALT-PRECORRIN-5A HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37477:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-5A HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21437">
        <property type="entry name" value="CbiG-like_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01890">
        <property type="entry name" value="CbiG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11760">
        <property type="entry name" value="CbiG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159672">
        <property type="entry name" value="CbiG N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159664">
        <property type="entry name" value="CobE/GbiG C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="6" id="KW-0002">3D-structure</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cobalamin synthesis G N-terminal" evidence="2">
        <location>
            <begin position="54"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="CbiG-like linker" evidence="3">
        <location>
            <begin position="137"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="CobE/GbiG C-terminal" evidence="1">
        <location>
            <begin position="216"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="60"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="6">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="3EEQ"/>
        </source>
    </evidence>
    <sequence length="337" mass="37665" checksum="988CC92C119A84F3" modified="2001-10-01" version="1">MESGGRLTMIENLWRGICIISASEDAFSAGETIKEKLKSFEIPVVHYRYKDAEIETIWKCYDAIVFVMALEGATRIVCKYAKSKTEDPAIVCIDDKINYVIPLLGGHWGANDIARELSVILNSTPIITTAAEIKGKLSIERIANILIAKIINPENIVKINAALLRDESICIDGIDVNVNFPENIKVNSEECSYIISLRGDKEYKDKIVVWLKPLKISIGIGSKKDVKMEEIRDGIYKVLERLNLKRERIGIIASIREEVKKIADEFNVRFRLVNEEEINNFMNPCLTPPSKTLIEVGLKGVAEISALIAGGRNSKLILRKIAISRNSTIAVATYEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WD3</accession>
    <name>Q97WD3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90399">
        <property type="entry name" value="H90399"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WD3"/>
    <dbReference type="PaxDb" id="273057-SSO2294"/>
    <dbReference type="EnsemblBacteria" id="AAK42455">
        <property type="protein sequence ID" value="AAK42455"/>
        <property type="gene ID" value="SSO2294"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2294"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2387"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134836_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WD3"/>
    <dbReference type="PhylomeDB" id="Q97WD3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="46"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="168" mass="19576" checksum="D73CB2E5D3D6EBAE" modified="2001-10-01" version="1">MEKFLDYDLVVYPLPKAINFLKALVSVKKIKKSNFIIRIVNLEPLFQRANELLRASKFNFNSGFYEIAAIAAVESLYLLLNATLIRIGADIPWYLDFDGLFRVISRYSNDDRIAEIRIKERDIISLLDDIKIRLGYSIPLELNEKDTEKLIKFAERIFDLLWGDLLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WH3</accession>
    <name>Q97WH3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90394">
        <property type="entry name" value="G90394"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991547.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WH3"/>
    <dbReference type="SMR" id="Q97WH3"/>
    <dbReference type="STRING" id="273057.SSO2246"/>
    <dbReference type="PaxDb" id="273057-SSO2246"/>
    <dbReference type="DNASU" id="1453741"/>
    <dbReference type="EnsemblBacteria" id="AAK42414">
        <property type="protein sequence ID" value="AAK42414"/>
        <property type="gene ID" value="SSO2246"/>
    </dbReference>
    <dbReference type="GeneID" id="1453741"/>
    <dbReference type="KEGG" id="sso:SSO2246"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2341"/>
    <dbReference type="eggNOG" id="arCOG01773">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_14_1_2"/>
    <dbReference type="InParanoid" id="Q97WH3"/>
    <dbReference type="PhylomeDB" id="Q97WH3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="40"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="223" mass="25709" checksum="2ED1F7F16F056595" modified="2001-10-01" version="1">MRAEDLFNDPKSYKRWYELHSKLYENERNVVRSFNLKDCLDVGSGPDIFHEEIGGRIVALDISLLMLKESKSDEKVLADALHLPFRDNSFKCVFSSVTVCFIEDVKGFIREMARITKERAIICFIARDSPWGEYYENLGKSGHKYYSHARFISRREFYVIINDFMKISRIRSTLKKMSETEIDGVYNDDSGSFICVEAIPMKPEPLSLSLNRHSGADPADPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WL4</accession>
    <name>Q97WL4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2202</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90389">
        <property type="entry name" value="E90389"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992090.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WL4"/>
    <dbReference type="STRING" id="273057.SSO2202"/>
    <dbReference type="PaxDb" id="273057-SSO2202"/>
    <dbReference type="EnsemblBacteria" id="AAK42372">
        <property type="protein sequence ID" value="AAK42372"/>
        <property type="gene ID" value="SSO2202"/>
    </dbReference>
    <dbReference type="GeneID" id="1453699"/>
    <dbReference type="KEGG" id="sso:SSO2202"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2298"/>
    <dbReference type="eggNOG" id="arCOG00996">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_873238_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WL4"/>
    <dbReference type="PhylomeDB" id="Q97WL4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="290" mass="33797" checksum="94482DCAD0B7DF8F" modified="2001-10-01" version="1">MKIILGIPDLKIPVWTFKQPLMINQLNWDRPSWENETWVDSGGYQIMVRGIKVEIEKVVEKYKILDATQYMSLDIPSIPCHKPSELNYKYFEYLYTVFDKKIVPVIHAYDPESIKKSIDFYSQYTDLVAFGGIVPPTLNKSGNKKLAVAIYYLVRKLWKGKIHVLGAGSPFMRKVYFNADSVDTSTYRVKGIHGMVIIPGKGERYVGNRKIVWGTKRASEEELEILLSFLDKTHYPFQIRFDNWIDRSLINAWVLLNSEYESNNPLIRYSKKLDTFAEDEIEEEVNELCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WQ7</accession>
    <name>Q97WQ7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acetyl-CoA C-acetyltransferase (Acetoacetyl-CoA thiolase) (AcaB-2)</fullName>
            <ecNumber evidence="3">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">acaB-2</name>
        <name evidence="3" type="ordered locus">SSO2061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.9" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90373">
        <property type="entry name" value="F90373"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988841.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WQ7"/>
    <dbReference type="FunCoup" id="Q97WQ7">
        <property type="interactions" value="167"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2061"/>
    <dbReference type="PaxDb" id="273057-SSO2061"/>
    <dbReference type="EnsemblBacteria" id="AAK42245">
        <property type="protein sequence ID" value="AAK42245"/>
        <property type="gene ID" value="SSO2061"/>
    </dbReference>
    <dbReference type="GeneID" id="1453572"/>
    <dbReference type="KEGG" id="sso:SSO2061"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2140"/>
    <dbReference type="eggNOG" id="arCOG01279">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_2_2_2"/>
    <dbReference type="InParanoid" id="Q97WQ7"/>
    <dbReference type="PhylomeDB" id="Q97WQ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004722">
        <property type="entry name" value="PRK06066.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF6">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase C-terminal" evidence="2">
        <location>
            <begin position="254"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="381" mass="41598" checksum="1A0B5FA2727CF6FA" modified="2001-10-01" version="1">MGTFLNRIAIIGLGWYGFKPTTHEVSFREMVFEAASRAYKDAGGINPRSDVDSFISCQEDFWEGISIADEFAPDQMGGAMRPTMTVAGDSLQGLAHAAMHINSGVANVVSIEAHSKVSDILTFSDLEKFAMDPIYIRSINPPNFHFIAGLDAVKFMHRKGITREDLALVVEKNKKAGLSSPRASFASNISAVDVMKKNYVVYPLSELDIAPFVDGAIVIVVADEEVAKKLKKDDYLVVKGIGFATDSSNLETAELGKANYMKIASDMAYKMAGVETPRRYFDAVFVDDRYSYKELQHLEGLRVSEEPSKDLREGNFSPQGEVPVNPLGGHMAKGVPLEASGFSLLLDAIEYIKQGKGERAVVASWRGIPTLTGSVVVVEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WW8</accession>
    <name>Q97WW8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1993</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90365">
        <property type="entry name" value="H90365"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992986.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WW8"/>
    <dbReference type="PaxDb" id="273057-SSO1993"/>
    <dbReference type="EnsemblBacteria" id="AAK42183">
        <property type="protein sequence ID" value="AAK42183"/>
        <property type="gene ID" value="SSO1993"/>
    </dbReference>
    <dbReference type="GeneID" id="1453510"/>
    <dbReference type="KEGG" id="sso:SSO1993"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2069"/>
    <dbReference type="eggNOG" id="arCOG07736">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631733_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WW8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="162" mass="18791" checksum="5F9595D273E868C8" modified="2001-10-01" version="1">MKIILAITEDKYGCSAIAELIRKLEENKKIENEIKLDCHSMGGKGNIISAKTVRIVKSSIFKGYDKIMIFVDSDNEDSQILENRIKDMLKRNGVDMNRVCIIVFNPHIEILLAPQENNPLEYLRTHDRYEKSDLPKRIRNIDLNTVYKLEVFQKIVKTLNDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WZ1</accession>
    <name>Q97WZ1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SpoVT-AbrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO9378</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90363">
        <property type="entry name" value="A90363"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991915.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WZ1"/>
    <dbReference type="PaxDb" id="273057-SSO9378"/>
    <dbReference type="EnsemblBacteria" id="AAK42160">
        <property type="protein sequence ID" value="AAK42160"/>
        <property type="gene ID" value="SSO9378"/>
    </dbReference>
    <dbReference type="GeneID" id="1453487"/>
    <dbReference type="KEGG" id="sso:SSO9378"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2043"/>
    <dbReference type="eggNOG" id="arCOG00820">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158484_9_2_2"/>
    <dbReference type="InParanoid" id="Q97WZ1"/>
    <dbReference type="PhylomeDB" id="Q97WZ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052975">
        <property type="entry name" value="Repressor-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860:SF7">
        <property type="entry name" value="TRANSCRIPTION REGULATOR, SPOVT_ABRB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="4"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="91" mass="10372" checksum="C7212281A99C94DF" modified="2001-10-01" version="1">MSLTLRVEVGKKGYIIIPKSVRDLVGIKEGDILILTVVGDKIILEPERKVNIREVVKKLEEHERKISYAKRASLGELENISLEEELKIDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X02</accession>
    <name>Q97X02_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transport membrane protein (Permease)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1958</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90361">
        <property type="entry name" value="F90361"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991753.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X02"/>
    <dbReference type="FunCoup" id="Q97X02">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1958"/>
    <dbReference type="PaxDb" id="273057-SSO1958"/>
    <dbReference type="EnsemblBacteria" id="AAK42149">
        <property type="protein sequence ID" value="AAK42149"/>
        <property type="gene ID" value="SSO1958"/>
    </dbReference>
    <dbReference type="GeneID" id="1453476"/>
    <dbReference type="KEGG" id="sso:SSO1958"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2032"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_762112_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X02"/>
    <dbReference type="PhylomeDB" id="Q97X02"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010645">
        <property type="entry name" value="MFS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23537">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23537:SF1">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="4"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="374" mass="41352" checksum="8BB348C7AE34C954" modified="2001-10-01" version="1">MLRNIIVSWIGTFLQLFLRLSWGVISLPIALVFHLNSIQIGLVATVFYIGYVVSSIPWGLIIDRIGPSSAVEYASILLVGMNLLLFLFLTSYAILLVAYLIEGLITAAIFPSAMKIVAVSYSNSSKFTFYVALVESAGPITIITLGIIASFLLHLWRFIYLIMAISFGLIAIFSHFNRIDVNRTEIKRSFKIILDRKITIATLIRLGELWSTWGTTTWIFPMLVLYRNISPTLSALFLLLFGVGQLVGILSVERLVERFGDRTVILINLIGFILLTFSIIFSNNIDILPEAFLLGIFSFSYRPPTDSLIMRIAGQSSAGTSIGYANAVSQIGTMIAPSFVGLTLYLTHSFSISMLALDVGCIISIISLLSLKHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X03</accession>
    <name>Q97X03_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90361">
        <property type="entry name" value="E90361"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991754.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X03"/>
    <dbReference type="STRING" id="273057.SSO1957"/>
    <dbReference type="PaxDb" id="273057-SSO1957"/>
    <dbReference type="EnsemblBacteria" id="AAK42148">
        <property type="protein sequence ID" value="AAK42148"/>
        <property type="gene ID" value="SSO1957"/>
    </dbReference>
    <dbReference type="GeneID" id="7809970"/>
    <dbReference type="KEGG" id="sso:SSO1957"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2030"/>
    <dbReference type="eggNOG" id="arCOG06000">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870834_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X03"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="136" mass="15304" checksum="57941BD3819D0DF2" modified="2001-10-01" version="1">MDLGKIIGLIVFISVLIVQFAMINNFNGLLQTTLNQINSTILILFTSQPKIGLVISNNQLILTFNNTLNFPITLLNVTGDYTYLSKPVTINPNEVKNISVVVTNLEMFERAIHDNSYNLTIVMKMFNTTFSQSEMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X17</accession>
    <name>Q97X17_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1936</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90359">
        <property type="entry name" value="D90359"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992190.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X17"/>
    <dbReference type="STRING" id="273057.SSO1936"/>
    <dbReference type="PaxDb" id="273057-SSO1936"/>
    <dbReference type="EnsemblBacteria" id="AAK42131">
        <property type="protein sequence ID" value="AAK42131"/>
        <property type="gene ID" value="SSO1936"/>
    </dbReference>
    <dbReference type="GeneID" id="7807176"/>
    <dbReference type="KEGG" id="sso:SSO1936"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2010"/>
    <dbReference type="eggNOG" id="arCOG02441">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1006930_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X17"/>
    <dbReference type="PhylomeDB" id="Q97X17"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37305">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37305:SF1">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="31072" checksum="AC156D53674B8C92" modified="2001-10-01" version="1">MFEIMLYEWRRAIARKKVIVLSIITFIFELGIYLAIYLAPSHSLKTLIIPLSPYLWALGVLLPQVLLIHFLAISIASGSMAEEYEQGTVDYFISKPITRLRFVMEKWLGSFSLLLIIYLFMVVLALILSSLLFGVQSELILLPELFFSIIFSTLVFLNIAFAIGEILRRSNLSFTISGFILIASIIVSDVLVFVSVITHNSNYETISEYLPTWGSTELPFMLLHNSPFSAIARGLNILPSISNNVSLAIVSIVVYSLIPLVLSIINFLGRDISKKVS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2021-09-29" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X43</accession>
    <name>TFS4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription factor S4</fullName>
            <shortName evidence="6">TFS4</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tfs4</name>
        <name evidence="5" type="synonym">rpoM-2</name>
        <name type="ordered locus">SSO9221</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="J. Virol." volume="82" first="4874" last="4883">
            <title>Transcriptome analysis of infection of the archaeon Sulfolobus solfataricus with Sulfolobus turreted icosahedral virus.</title>
            <authorList>
                <person name="Ortmann A.C."/>
                <person name="Brumfield S.K."/>
                <person name="Walther J."/>
                <person name="McInnerney K."/>
                <person name="Brouns S.J."/>
                <person name="van de Werken H.J."/>
                <person name="Bothner B."/>
                <person name="Douglas T."/>
                <person name="van de Oost J."/>
                <person name="Young M.J."/>
            </authorList>
            <dbReference type="PubMed" id="18337566"/>
            <dbReference type="DOI" id="10.1128/jvi.02583-07"/>
        </citation>
        <scope>INDUCTION BY VIRUS</scope>
        <source>
            <strain>2-2-12</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2017" name="Nat. Commun." volume="8" first="1914" last="1914">
            <title>The transcript cleavage factor paralogue TFS4 is a potent RNA polymerase inhibitor.</title>
            <authorList>
                <person name="Fouqueau T."/>
                <person name="Blombach F."/>
                <person name="Hartman R."/>
                <person name="Cheung A.C.M."/>
                <person name="Young M.J."/>
                <person name="Werner F."/>
            </authorList>
            <dbReference type="PubMed" id="29203770"/>
            <dbReference type="DOI" id="10.1038/s41467-017-02081-3"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>PROBABLE COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>INDUCTION BY VIRUS</scope>
        <scope>DOMAIN</scope>
        <scope>ACTIVE SITE</scope>
        <scope>MUTAGENESIS OF 72-ILE--LYS-78; 76-LYS--LYS-78; 76-LYS-LYS-77; LYS-76; 77-LYS-LYS-78; LYS-77 AND LYS-78</scope>
        <source>
            <strain>2-2-12</strain>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="journal article" date="2021" name="Nat. Commun." volume="12" first="5523" last="5523">
            <title>Structural basis of RNA polymerase inhibition by viral and host factors.</title>
            <authorList>
                <person name="Pilotto S."/>
                <person name="Fouqueau T."/>
                <person name="Lukoyanova N."/>
                <person name="Sheppard C."/>
                <person name="Lucas-Staat S."/>
                <person name="Diaz-Santin L.M."/>
                <person name="Matelska D."/>
                <person name="Prangishvili D."/>
                <person name="Cheung A.C.M."/>
                <person name="Werner F."/>
            </authorList>
            <dbReference type="PubMed" id="34535646"/>
            <dbReference type="DOI" id="10.1038/s41467-021-25666-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.61 ANGSTROMS) IN COMPLEX WITH HOST (S.ACIDOCALDARIUS) RNA POLYMERASE</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>DOMAIN</scope>
        <source>
            <strain>2-2-12</strain>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">A potent inhibitor of RNA polymerase (RNAP) probably involved in viral defense. Destabilizes the transcription pre-initiation complex of TBP, TFB, DNA and RNAP, inhibits abortive transcription initiation, productive initiation and transcription elongation. Increases the RNAP KM for NTPs about 50-fold. Overexpression of TFS1-tip4 (TFS1 with the active tip of this protein, phenocopies this protein) in S.acidocaldarius MW001 leads to severe growth inhibition (PubMed:29203770). When bound to RNAP induces conformational changes that widen the DNA-binding channel, probably destabilizing the interaction of DNA with RNAP (PubMed:34535646).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 8 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4 8 9">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 4">Interacts with RNA polymerase.</text>
    </comment>
    <comment type="induction">
        <text evidence="2 3">At least 4-fold induced following infection by Sulfolobus turreted icosahedral virus 1 (STIV-1) in strain 2-2-12 (PubMed:18337566). Undetected in non-infected cells. Induced following STIV-1 infection, protein is detectable by 12 hours post-infection (p.i.) and increases until at least 24 hours p.i. in strain 2-2-12 (at protein level) (PubMed:29203770).</text>
    </comment>
    <comment type="domain">
        <text evidence="3 4 8">Has 2 zinc-ribbon domains (ZR-N and ZR-C); functionally important residues Lys-76, Lys-77 and Lys-78 are probably located at the tip of a domain that extends from ZR-C into the RNAP active site. The nature of these residues determines if the protein is involved in stalled transcript cleavage and thus activation (Asp and Glu residues in TFS1) or in RNAP inhibition (Lys or Arg residues in this protein TFS4). Increasing numbers of Lys residues lead to an increased ability to inhibit transcription. ZR-N interacts with Rpo1C and Rpo2 while ZR-C binds in the NTP entry funnel to sites in Rpo1N without reaching the active site (PubMed:34535646).</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">Strain 2-2-12 is a substrain of P2 that is highly susceptible to infection by Sulfolobus turreted icosahedral virus 1 (STIV-1).</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90356">
        <property type="entry name" value="B90356"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923647.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="7OQY">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.61 A"/>
        <property type="chains" value="Y=1-92"/>
    </dbReference>
    <dbReference type="PDBsum" id="7OQY"/>
    <dbReference type="AlphaFoldDB" id="Q97X43"/>
    <dbReference type="EMDB" id="EMD-13034"/>
    <dbReference type="SMR" id="Q97X43"/>
    <dbReference type="STRING" id="273057.SSO9221"/>
    <dbReference type="PaxDb" id="273057-SSO9221"/>
    <dbReference type="EnsemblBacteria" id="AAK42105">
        <property type="protein sequence ID" value="AAK42105"/>
        <property type="gene ID" value="SSO9221"/>
    </dbReference>
    <dbReference type="GeneID" id="1453432"/>
    <dbReference type="KEGG" id="sso:SSO9221"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1974"/>
    <dbReference type="eggNOG" id="arCOG00579">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2406498_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X43"/>
    <dbReference type="PhylomeDB" id="Q97X43"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047723">
        <property type="entry name" value="TransFacS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000453930" description="Transcription factor S4">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="ZR-N" evidence="4 8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Flexible linker" evidence="4">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="ZR-C" evidence="4 8">
        <location>
            <begin position="55"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 4 8">
        <location>
            <position position="4"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 4 8">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 4 8">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 4 8">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8 9">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8 9">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8 9">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8 9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Does not inhibit RNAP activity, has acquired transcript cleavage activity (TFS4-tip1, with active tip of TFS1)." evidence="3">
        <original>ILLNKKK</original>
        <variation>YFWILQTRRADEPPT</variation>
        <location>
            <begin position="72"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No longer inhibits RNAP activity." evidence="3">
        <original>KKK</original>
        <variation>AAA</variation>
        <location>
            <begin position="76"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Mild decrease in RNAP activity." evidence="3">
        <original>KKK</original>
        <variation>AKA</variation>
        <location>
            <begin position="76"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Wild-type inhibition of RNAP activity." evidence="3">
        <original>KKK</original>
        <variation>RRR</variation>
        <location>
            <begin position="76"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Small decrease in RNAP activity." evidence="3">
        <original>KK</original>
        <variation>AA</variation>
        <location>
            <begin position="76"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Very small decrease in RNAP activity." evidence="3">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Medium decrease in RNAP activity." evidence="3">
        <original>KK</original>
        <variation>AA</variation>
        <location>
            <begin position="77"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Very small decrease in RNAP activity." evidence="3">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Mild decrease in RNAP activity." evidence="3">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="turn" evidence="12">
        <location>
            <begin position="5"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="17"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="turn" evidence="12">
        <location>
            <begin position="21"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="34"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="58"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="64"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="69"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="turn" evidence="12">
        <location>
            <begin position="76"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="80"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="turn" evidence="12">
        <location>
            <begin position="84"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="89"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18337566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="29203770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="34535646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="11427726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="6">
        <source>
            <dbReference type="PubMed" id="29203770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <evidence type="ECO:0000305" key="8">
        <source>
            <dbReference type="PubMed" id="29203770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="9">
        <source>
            <dbReference type="PDB" id="7OQY"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="12">
        <source>
            <dbReference type="PDB" id="7OQY"/>
        </source>
    </evidence>
    <sequence length="92" mass="10403" checksum="88FB7493ECCC4416" modified="2001-10-01" version="1">MRFCPKCGSFLKVKGNKMVCSKCGYSDHDVEKVILKENVAHENDKTIIADGETIEGRVAISLCPRCGSVRAILLNKKKRLYRCMTCNFVYNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XF9</accession>
    <name>Q97XF9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">dTDP-4-dehydrorhamnose reductase (RfbD-2)</fullName>
            <ecNumber evidence="2">1.1.1.133</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rfbD-2</name>
        <name evidence="2" type="ordered locus">SSO1783</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.133" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90339">
        <property type="entry name" value="H90339"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XF9"/>
    <dbReference type="FunCoup" id="Q97XF9">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1783"/>
    <dbReference type="PaxDb" id="273057-SSO1783"/>
    <dbReference type="EnsemblBacteria" id="AAK41975">
        <property type="protein sequence ID" value="AAK41975"/>
        <property type="gene ID" value="SSO1783"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1783"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1833"/>
    <dbReference type="eggNOG" id="arCOG01367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045518_2_4_2"/>
    <dbReference type="InParanoid" id="Q97XF9"/>
    <dbReference type="PhylomeDB" id="Q97XF9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008831">
        <property type="term" value="F:dTDP-4-dehydrorhamnose reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05254">
        <property type="entry name" value="dTDP_HR_like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43242">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43242:SF1">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RmlD-like substrate binding" evidence="1">
        <location>
            <begin position="2"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="207" mass="23601" checksum="8DA954786EC2B3D3" modified="2001-10-01" version="1">MTDVDKCEIEKDKAYKINSQAVKHIVRAGRVVDSYVIHISTDYVFDGNKGNYKEDNIPNPINYYGLSKLLGEIFASSYDESLIIRTSGVFRNKGFPIYVYKTLKEGKTVLAFKGYYSPISARKLALAIDELLDLRKTGIIHVAGERISRYELAIKIKEKFNLPGEVKEVDEIKSWIAKRPYDSSLDISKARKLLTIDFYTLDLEGMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XL9</accession>
    <name>Q97XL9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase in ISC1395</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1695</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90331">
        <property type="entry name" value="A90331"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923671.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XL9"/>
    <dbReference type="STRING" id="273057.SSO1695"/>
    <dbReference type="PaxDb" id="273057-SSO1695"/>
    <dbReference type="EnsemblBacteria" id="AAK41904">
        <property type="protein sequence ID" value="AAK41904"/>
        <property type="gene ID" value="SSO1695"/>
    </dbReference>
    <dbReference type="GeneID" id="1454683"/>
    <dbReference type="KEGG" id="sso:SSO1695"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1747"/>
    <dbReference type="eggNOG" id="arCOG02127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056788_0_1_2"/>
    <dbReference type="InParanoid" id="Q97XL9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13551">
        <property type="entry name" value="HTH_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="2">
        <location>
            <begin position="157"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="298" mass="35029" checksum="CA5C33191631321C" modified="2001-10-01" version="1">MDVLRTLKRRIRKLIEKKPKNLNETKARGILLYLKGMNTRKIAKILQVHLSTVYRWINQFEKEGEKCLFYKERKGRNKKIDEKEIKVEELQGKTIWEAKAYIEEKFHVKLSHVTAWRIARKRLKIPYIKPYNKKRPFNADNILRGRLKGVIKKGVKVFFMDESGIHHDPSRRLGLYNVSVDYPSKKLNVLASIPLFDGVPCFMSTYSNVNFKIFAEFLRLFRNSGPIVLILDNAKFHKNAYVFSVASQLGITLLFLPPYSPDLNPIELVWRDLVRGLILIIILNTLLIRWRRSSIICF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XP4</accession>
    <name>Q97XP4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF917 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1667</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90327">
        <property type="entry name" value="H90327"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990101.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XP4"/>
    <dbReference type="STRING" id="273057.SSO1667"/>
    <dbReference type="PaxDb" id="273057-SSO1667"/>
    <dbReference type="EnsemblBacteria" id="AAK41879">
        <property type="protein sequence ID" value="AAK41879"/>
        <property type="gene ID" value="SSO1667"/>
    </dbReference>
    <dbReference type="GeneID" id="1454658"/>
    <dbReference type="KEGG" id="sso:SSO1667"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1716"/>
    <dbReference type="eggNOG" id="arCOG03841">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038930_0_1_2"/>
    <dbReference type="InParanoid" id="Q97XP4"/>
    <dbReference type="PhylomeDB" id="Q97XP4"/>
    <dbReference type="BRENDA" id="3.5.2.9">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.390.10">
        <property type="entry name" value="CV3147-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1610.10">
        <property type="entry name" value="CV3147-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048350">
        <property type="entry name" value="S-Me-THD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024071">
        <property type="entry name" value="S-Me-THD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010318">
        <property type="entry name" value="S-Me-THD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027479">
        <property type="entry name" value="S-Me-THD_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20906">
        <property type="entry name" value="S-Me-THD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06032">
        <property type="entry name" value="S-Me-THD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160991">
        <property type="entry name" value="CV3147-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-Me-THD N-terminal" evidence="1">
        <location>
            <begin position="10"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="S-Me-THD-like C-terminal" evidence="2">
        <location>
            <begin position="170"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="39953" checksum="90256769BA05B15D" modified="2001-10-01" version="1">METYFIEERDLQNLITGASFLGTGGGGDPYIGGLMLKRELEIVKKIEVVTGMKYSNSKSFVLGSAMMGAPIVMIEKIPNSSEAKKALEVYSKFTDNEVEYITPLEMGGVNSTIPLITSAKTGIPVVDGDGMGRAFPELQMTTFYFYGVQPSPIVIFDERGNVSIVQGIDGYWSEKIARIITVRYGGSAYIALYGTSLPTYLNTAVLGTLSLALEIGKLLNQMRLDDLLDLLKAIIMFKGKVVDVQRRVERGFSKGNVIIDGIENYSEKTMKIDFQNEFLVARINNDIVTTVPDLITVLDLFTLKPITTDRIKYGQKVMVIGIPSSGKLRTKEALRYVGPRAFGYDVDFIPLEERWKKVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XR4</accession>
    <name>Q97XR4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alcohol dehydrogenase (Zn containing) (Adh-5)</fullName>
            <ecNumber evidence="8">1.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">adh-5</name>
        <name evidence="8" type="ordered locus">SSO1646</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90325">
        <property type="entry name" value="D90325"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990900.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XR4"/>
    <dbReference type="FunCoup" id="Q97XR4">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1646"/>
    <dbReference type="PaxDb" id="273057-SSO1646"/>
    <dbReference type="EnsemblBacteria" id="AAK41859">
        <property type="protein sequence ID" value="AAK41859"/>
        <property type="gene ID" value="SSO1646"/>
    </dbReference>
    <dbReference type="GeneID" id="1454638"/>
    <dbReference type="KEGG" id="sso:SSO1646"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1694"/>
    <dbReference type="eggNOG" id="arCOG01455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_2_2"/>
    <dbReference type="InParanoid" id="Q97XR4"/>
    <dbReference type="PhylomeDB" id="Q97XR4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05284">
        <property type="entry name" value="arabinose_DH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="11"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="344" mass="37253" checksum="F92B25FD4435EE60" modified="2001-10-01" version="1">MKAVRIHEYNKPLSLDEVNKPRVSGPYDIVVRIRGAGVCRTDLHIIEGVWKDVLNPKLPFTIGHENVGIIEEKGNAVDWLDVGDQVILHPYITCRHCRACRSGNDMHCPYGKFPGLDGTDGGYAEYLRTSAFSAIKIPKGIDIIPMAPLADAGITAYHAVKKISQYLYPGSIVGVIGVGGLGHIGIQALKAITPARVIAIDISEEKTKLAKELGADEVVIAGKDSGVSDVLKLTDNIGVDAIIDFVGEHSTPQNALRMIRKGGIYSIVGYGGEFKNSTLDFINREIMVVGNLVGTYNELSELVTLYVQNKVKLKVKTFPLEEANSALEELKAGRILGRAVLVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XS5</accession>
    <name>Q97XS5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8549</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90323">
        <property type="entry name" value="H90323"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923534.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XS5"/>
    <dbReference type="STRING" id="273057.SSO8549"/>
    <dbReference type="PaxDb" id="273057-SSO8549"/>
    <dbReference type="EnsemblBacteria" id="AAK41847">
        <property type="protein sequence ID" value="AAK41847"/>
        <property type="gene ID" value="SSO8549"/>
    </dbReference>
    <dbReference type="GeneID" id="54123716"/>
    <dbReference type="KEGG" id="sso:SSO8549"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1678"/>
    <dbReference type="eggNOG" id="arCOG07229">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2821080_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XS5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="67" mass="7922" checksum="10063EEEC8AB0793" modified="2001-10-01" version="1">MIDPKLIIKLLEDRQRPRGSKIKDEDLKRLAKFGEENMKLLEVLGCWKMEGDIIYYKTGCLGNYFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XT6</accession>
    <name>Q97XT6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyltransferase family 1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1621</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90322">
        <property type="entry name" value="E90322"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923523.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XT6"/>
    <dbReference type="SMR" id="Q97XT6"/>
    <dbReference type="STRING" id="273057.SSO1621"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1621"/>
    <dbReference type="EnsemblBacteria" id="AAK41836">
        <property type="protein sequence ID" value="AAK41836"/>
        <property type="gene ID" value="SSO1621"/>
    </dbReference>
    <dbReference type="GeneID" id="1454615"/>
    <dbReference type="KEGG" id="sso:SSO1621"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1663"/>
    <dbReference type="eggNOG" id="arCOG01411">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_822879_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XT6"/>
    <dbReference type="PhylomeDB" id="Q97XT6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="3">
        <location>
            <begin position="65"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="158"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="38936" checksum="D65AC49056080B8C" modified="2001-10-01" version="1">MRVLLIAGKDDFNEDVSKVTGIGRYVREVYEGLKKQIDVEAYPLFDYSSIFSSINSIIKGTFHDYSSYDIIHLLSPKPFFPLRKGKAKWITTVHDLFFLKYNESKPSPFMQKIYLKSILSSDAIIAVSSLVKEDVEKLGYKKKIFVINPGIGEEFFTSPKKPEKKDVIKIGYIGRLDSERKDLIRGIEVFRKIKEKNVIFELWGNYNPDSEIFKRIKKESEEDPRIRIMGPAPDEKLVEIYDSFDAFFFPTKEEGFGLPIVEAQARGVPVIVFKDARIPEEACNYCIRINEEFPQIDYIKSFKEKYNDALRKYASKFSLGISVKNILNSYGSIMLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XU7</accession>
    <name>Q97XU7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO8478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90320">
        <property type="entry name" value="H90320"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XU7"/>
    <dbReference type="STRING" id="273057.SSO8478"/>
    <dbReference type="PaxDb" id="273057-SSO8478"/>
    <dbReference type="DNASU" id="1454602"/>
    <dbReference type="EnsemblBacteria" id="AAK41823">
        <property type="protein sequence ID" value="AAK41823"/>
        <property type="gene ID" value="SSO8478"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8478"/>
    <dbReference type="eggNOG" id="arCOG07733">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2406496_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XU7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="95" mass="10805" checksum="3B42CDBF11EE2B43" modified="2001-10-01" version="1">MEFLSLLIFVSGIIVAFLTLYFSPDFGRVKPNKRSLSTIFLSIALTGIGLWLYSIDHSTYFCPNINAPQIHLVYFVEGMRSRHLCTLTYGHSQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XU8</accession>
    <name>Q97XU8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90320">
        <property type="entry name" value="G90320"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923511.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XU8"/>
    <dbReference type="STRING" id="273057.SSO8475"/>
    <dbReference type="PaxDb" id="273057-SSO8475"/>
    <dbReference type="EnsemblBacteria" id="AAK41822">
        <property type="protein sequence ID" value="AAK41822"/>
        <property type="gene ID" value="SSO8475"/>
    </dbReference>
    <dbReference type="GeneID" id="1454601"/>
    <dbReference type="KEGG" id="sso:SSO8475"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1649"/>
    <dbReference type="eggNOG" id="arCOG08408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2327376_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XU8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="95" mass="10676" checksum="2C63472CAA3D215E" modified="2001-10-01" version="1">MWINVAPEPINAVAGHSSATDVGGTTPIPSYYAETGLTLFHNVFINYWYNPYAWYFSIGTPQDYSAHGLNYKQLSEGQSYNTINGLNYALYWISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XW8</accession>
    <name>Q97XW8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transporter (Proton symporter)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90318">
        <property type="entry name" value="B90318"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923491.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XW8"/>
    <dbReference type="STRING" id="273057.SSO1591"/>
    <dbReference type="PaxDb" id="273057-SSO1591"/>
    <dbReference type="DNASU" id="1454580"/>
    <dbReference type="EnsemblBacteria" id="AAK41801">
        <property type="protein sequence ID" value="AAK41801"/>
        <property type="gene ID" value="SSO1591"/>
    </dbReference>
    <dbReference type="GeneID" id="7806180"/>
    <dbReference type="KEGG" id="sso:SSO1591"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1626"/>
    <dbReference type="eggNOG" id="arCOG05339">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1127122_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XW8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="232" mass="26116" checksum="917835241091EBC4" modified="2001-10-01" version="1">MSFDPIYFLTPIIVMVFSFGMVYKLVKSIKVLMFSALAYFIAIVLKVLVQFFTLGYLASINSLAVLGLYYGLQTSIFEVGLAYVFALLGKLKNGWGYGVSLAMWENGVLISIPTLFIYIAYYFFPLILSTPSQLEYPITQALPLIGLSILERVSSLLIHSSWGYLSVLSVITGRKRLILVAFPMGFVDFLVPFEKYLGILTFEFIFFCIGLICLVMAWSLGKYYRSTSSKDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XZ0</accession>
    <name>Q97XZ0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1160</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO8380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK67521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XZ0"/>
    <dbReference type="STRING" id="273057.SSO8380"/>
    <dbReference type="PaxDb" id="273057-SSO8380"/>
    <dbReference type="EnsemblBacteria" id="AAK67521">
        <property type="protein sequence ID" value="AAK67521"/>
        <property type="gene ID" value="SSO8380"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8380"/>
    <dbReference type="HOGENOM" id="CLU_2476185_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XZ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK67521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="87" mass="9920" checksum="ED0052BD1B1188C4" modified="2001-10-01" version="1">MKGKILGEEICVTKSEFQMIILNFPHPNSSNKGRKGEEVAKTLVTAVERLNREQVKDVQDLKPNSEKLRREKRSSREITRASEATIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y09</accession>
    <name>Q97Y09_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-oxoacyl-(Acyl carrier protein) reductase (FabG-3)</fullName>
            <ecNumber evidence="3">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fabG-3</name>
        <name evidence="3" type="ordered locus">SSO1542</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90313">
        <property type="entry name" value="C90313"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y09"/>
    <dbReference type="FunCoup" id="Q97Y09">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1542"/>
    <dbReference type="PaxDb" id="273057-SSO1542"/>
    <dbReference type="EnsemblBacteria" id="AAK41762">
        <property type="protein sequence ID" value="AAK41762"/>
        <property type="gene ID" value="SSO1542"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1542"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1579"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_2"/>
    <dbReference type="InParanoid" id="Q97Y09"/>
    <dbReference type="PhylomeDB" id="Q97Y09"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24321">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24321:SF8">
        <property type="entry name" value="ESTRADIOL 17-BETA-DEHYDROGENASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="255" mass="28186" checksum="A052EB569313F45F" modified="2001-10-01" version="1">MIRMELFSLKGRTAVVIGAGSGIGKAIAKLFTDLGGKVVASDVKGLEDLQSTYDTFKADITNVNDVRQLVEFSLNKLGEIYALYITPSINIRKRIENYTLDEFDRVVNLNLRGNFIVLKEFLKVMKENKNGGSIVLFSSIRHLVTEPGQAVYASTKAAIVQLAKTAAAEYGKYGLRVNVIAPGVVDTPFTQQIKNNPEWYRAYTEKTILKRWADPMEIASVAVFLAMPASSYITGTVIYVDGGWTAIDGRYEPDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y17</accession>
    <name>Q97Y17_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Luciferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90312">
        <property type="entry name" value="C90312"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989859.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y17"/>
    <dbReference type="STRING" id="273057.SSO1532"/>
    <dbReference type="PaxDb" id="273057-SSO1532"/>
    <dbReference type="EnsemblBacteria" id="AAK41754">
        <property type="protein sequence ID" value="AAK41754"/>
        <property type="gene ID" value="SSO1532"/>
    </dbReference>
    <dbReference type="GeneID" id="1454533"/>
    <dbReference type="KEGG" id="sso:SSO1532"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1571"/>
    <dbReference type="eggNOG" id="arCOG02410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_3_0_2"/>
    <dbReference type="InParanoid" id="Q97Y17"/>
    <dbReference type="PhylomeDB" id="Q97Y17"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050766">
        <property type="entry name" value="Bact_Lucif_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30137">
        <property type="entry name" value="LUCIFERASE-LIKE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30137:SF6">
        <property type="entry name" value="LUCIFERASE-LIKE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="1">
        <location>
            <begin position="25"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="341" mass="39215" checksum="FCAD9CB7D9286636" modified="2001-10-01" version="1">MVENKIKPELFWVMDYYSESGLTKREYYEQVAKEIEFAEKLGYRGVWIAEHHFIDYGIVPSTSVYLSHIASRTKNIRIGPAVATIVFKNPIQVAEEYSLLDTLSNGRLNLAMGSGYLKHEFAGFNIPQEAKREIFDEALDIILKLMSGESVSVNGKYFKYENIKINVLPLQKPYPPIWIAVLRAEAAYHVGKKGFNLMMIPYATVDKIDDVKPVTDSYKQGLKENTNAEISLAFHTHISKSFHDAVEESKEYLHRYVFSRLYAKRRTLDDLYNSGLLLLGGPEDVANQIYKIWKLTEVNRLMFLVDYGMKPLALIQDILINIREKVAKLLEERGIELDFIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y55</accession>
    <name>Q97Y55_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial transposase from ISC1048</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="E90307"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y55"/>
    <dbReference type="PaxDb" id="273057-SSO1490"/>
    <dbReference type="EnsemblBacteria" id="AAK41716">
        <property type="protein sequence ID" value="AAK41716"/>
        <property type="gene ID" value="SSO1490"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1490"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1530"/>
    <dbReference type="eggNOG" id="arCOG02130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2353342_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y55"/>
    <dbReference type="PhylomeDB" id="Q97Y55"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="10"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="104" mass="12306" checksum="923BBBF997745F1B" modified="2001-10-01" version="1">MRYFKRRVGSGRVYMVMDNYSPHKTKGTLEVCCRKGIHPVFTPPYSPELNMAEAVFKSLKNYMSNKIFYTIEDVKNCIKQFFEENKYRFNLNAITYLGLDKIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YB5</accession>
    <name>Q97YB5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Partial transposase ISC1250</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1414</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90299">
        <property type="entry name" value="A90299"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YB5"/>
    <dbReference type="PaxDb" id="273057-SSO1414"/>
    <dbReference type="EnsemblBacteria" id="AAK41648">
        <property type="protein sequence ID" value="AAK41648"/>
        <property type="gene ID" value="SSO1414"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1414"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1438"/>
    <dbReference type="eggNOG" id="arCOG04918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129669_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YB5"/>
    <dbReference type="PhylomeDB" id="Q97YB5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217:SF7">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="178" mass="20469" checksum="0ED38627BE306D17" modified="2001-10-01" version="1">MDLLTANEITLYGIQLKELIEGPGQSLTLTLAPKILSDDALLNKSVQEIEKMALEEAEKRSPNYRRGKEVKRKGYARYRFLKGFKIMLEGREVIYKLEWISVELPVLYGIKGRVKTQVEETLLRKERKVFATLMLVYSVLGGKLNAKLWMPQIEVSGNFKYVIVDGKCVSILEKFLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YI7</accession>
    <name>Q97YI7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">malate synthase</fullName>
            <ecNumber evidence="2">2.3.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">aceB/mas</name>
        <name evidence="4" type="ordered locus">SSO1334</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the malate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.9" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90289">
        <property type="entry name" value="E90289"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923329.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YI7"/>
    <dbReference type="FunCoup" id="Q97YI7">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1334"/>
    <dbReference type="PaxDb" id="273057-SSO1334"/>
    <dbReference type="EnsemblBacteria" id="AAK41572">
        <property type="protein sequence ID" value="AAK41572"/>
        <property type="gene ID" value="SSO1334"/>
    </dbReference>
    <dbReference type="GeneID" id="1454351"/>
    <dbReference type="KEGG" id="sso:SSO1334"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1340"/>
    <dbReference type="eggNOG" id="arCOG05498">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_342775_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YI7"/>
    <dbReference type="BioCyc" id="MetaCyc:MONOMER-18076"/>
    <dbReference type="BRENDA" id="2.3.3.9">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004474">
        <property type="term" value="F:malate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00480">
        <property type="entry name" value="malate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.360:FF:000005">
        <property type="entry name" value="Malate synthase (GlcB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.360">
        <property type="entry name" value="Malate synthase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011076">
        <property type="entry name" value="Malate_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006252">
        <property type="entry name" value="Malate_synthA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001465">
        <property type="entry name" value="Malate_synthase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046363">
        <property type="entry name" value="MS_N_TIM-barrel_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902">
        <property type="entry name" value="MALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902:SF1">
        <property type="entry name" value="MALATE SYNTHASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01274">
        <property type="entry name" value="MS_TIM-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51645">
        <property type="entry name" value="Malate synthase G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Malate synthase TIM barrel" evidence="3">
        <location>
            <begin position="211"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="826" mass="94702" checksum="6B400E4090E53EAF" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-01-24" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YK5</accession>
    <name>Q97YK5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90287">
        <property type="entry name" value="C90287"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923311.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YK5"/>
    <dbReference type="STRING" id="273057.SSO1317"/>
    <dbReference type="PaxDb" id="273057-SSO1317"/>
    <dbReference type="EnsemblBacteria" id="AAK41554">
        <property type="protein sequence ID" value="AAK41554"/>
        <property type="gene ID" value="SSO1317"/>
    </dbReference>
    <dbReference type="GeneID" id="1454333"/>
    <dbReference type="KEGG" id="sso:SSO1317"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1319"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YK5"/>
    <dbReference type="PhylomeDB" id="Q97YK5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="80"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37388" checksum="CE00969E28A5FE4F" modified="2001-10-01" version="1">MQTMILSKTELKSLAITLATNNINVISQDLDPEIVKAAPSLLTGNRGKYYLKVVRRGEKVISKGQRTFKFYPIYREVKGEINVVAVDETGLTVGEKEQEKAEGFLLYNWKRKGVKMRSLDLVYPLRLPLLVEVADLRSDSPSQFLLRSVREVSQYMEIDYVVADAGFLNLGVIKEMPVKTIVRGKSNLKGFKELSNVPLVEKRYEVKDKVYVAYRVLEFEGLYYYDVVYVKGKPRHFMFVTNFEGDPYELAELYRLRWQVEEGFKVRKARIRYVRKLSNKIFLFLYYTVLDSAWNLVNHLLFNFKSTCKKVLSFDSFVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YL2</accession>
    <name>Q97YL2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Enoyl CoA hydratase (PaaF-3)</fullName>
            <ecNumber evidence="4">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">paaF-3</name>
        <name evidence="4" type="ordered locus">SSO1311</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90286">
        <property type="entry name" value="D90286"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923304.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YL2"/>
    <dbReference type="STRING" id="273057.SSO1311"/>
    <dbReference type="PaxDb" id="273057-SSO1311"/>
    <dbReference type="EnsemblBacteria" id="AAK41547">
        <property type="protein sequence ID" value="AAK41547"/>
        <property type="gene ID" value="SSO1311"/>
    </dbReference>
    <dbReference type="GeneID" id="1454326"/>
    <dbReference type="KEGG" id="sso:SSO1311"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1312"/>
    <dbReference type="eggNOG" id="arCOG00239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_2"/>
    <dbReference type="InParanoid" id="Q97YL2"/>
    <dbReference type="PhylomeDB" id="Q97YL2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="267" mass="29594" checksum="7F429186BB18DBBE" modified="2001-10-01" version="1">MGLTELTPKYFKIEVEDGVGIIRLNRPPANAHNLEMLRELDNIIVESRFDENVKALIITSAIPRFFSAGFDINEIKDKSPEYIGLSSQFSKEVMLRMMSTKKLIIASINGHCMGGGLELALACDLRFGANDENIKFGMPEVANLALIPGEGGTQFLARLVGRSKAIYLMITGKTLSPKEAYDLGILDKLVEPEKLFEESLEFARQISRGPSLAVGFAKLAVNEGMDLPLYNAFALEREMQNQALASEDAKEGARAFFEKRRPNFKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YN4</accession>
    <name>Q97YN4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99284">
        <property type="entry name" value="A99284"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991991.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YN4"/>
    <dbReference type="PaxDb" id="273057-SSO1290"/>
    <dbReference type="EnsemblBacteria" id="AAK41528">
        <property type="protein sequence ID" value="AAK41528"/>
        <property type="gene ID" value="SSO1290"/>
    </dbReference>
    <dbReference type="GeneID" id="1454307"/>
    <dbReference type="KEGG" id="sso:SSO1290"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1289"/>
    <dbReference type="eggNOG" id="arCOG05398">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084677_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YN4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="7"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="324" mass="38074" checksum="462274FE42123F74" modified="2001-10-01" version="1">MNYCIYATVFNNAPTLEESVKSVWRSDVTIVITDNYSTDSTWERLLELRKDYNLVLYRLKSTRGKGRGYSLKHCPENSITTYFDLDMRYNESFHKILEWAPRDKKILVNLVNGFVVKRETILEKGSWRDLNRAEDWEIVSRVGFDYFVPALIHAELRNELAREKRYAKGLKYYGRRFKNKLDVIRGLGYNWSDMKIAYSQHSIPYKIFVNAPSFVLAKLMGIYRNYKEYNNGVGTILSALDRMIDLKEIGINDKYFLFGGYWGFFSAYNLDKIIDEKLPSKVGRVRKLICNDDGLRYIKTLDGFDIIKLASFLKDKLECKEFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YR9</accession>
    <name>Q97YR9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90279">
        <property type="entry name" value="B90279"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YR9"/>
    <dbReference type="PaxDb" id="273057-SSO1250"/>
    <dbReference type="EnsemblBacteria" id="AAK41489">
        <property type="protein sequence ID" value="AAK41489"/>
        <property type="gene ID" value="SSO1250"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1250"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1244"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113185_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YR9"/>
    <dbReference type="PhylomeDB" id="Q97YR9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="202" mass="23151" checksum="29E78B65FDB45A0D" modified="2001-10-01" version="1">MNKIPAIQQYYHALKETIQLLADPMTDVRKDTLAKLVLGGVMGGTATEIAQVIGMDYETVLKNLDKLANVNLIKAVKEIVKDHPVLLIIDDTHDHKEYARAIPVSRNGAQVFYCREHKRYEPAIQLLLITVKDLRTNEAYVVTIIPYIPQKVVEVLRERGEEVEFKTKIQEYLEILPVLEKEFSVVCKVFDSWYVSMGISFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YV0</accession>
    <name>Q97YV0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="3">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">porB-1</name>
        <name evidence="7" type="ordered locus">SSO1206</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90274">
        <property type="entry name" value="F90274"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YV0"/>
    <dbReference type="FunCoup" id="Q97YV0">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1206"/>
    <dbReference type="PaxDb" id="273057-SSO1206"/>
    <dbReference type="EnsemblBacteria" id="AAK41453">
        <property type="protein sequence ID" value="AAK41453"/>
        <property type="gene ID" value="SSO1206"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1206"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1204"/>
    <dbReference type="eggNOG" id="arCOG01601">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058423_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YV0"/>
    <dbReference type="PhylomeDB" id="Q97YV0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03376">
        <property type="entry name" value="TPP_PFOR_porB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051479">
        <property type="entry name" value="PorB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008818">
        <property type="entry name" value="PRK11864.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42897:SF1">
        <property type="entry name" value="2-OXOACID OXIDOREDUCTASE (FERREDOXIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42897">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="6">
        <location>
            <begin position="69"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="300" mass="33069" checksum="05CA20307E2C581F" modified="2001-10-01" version="1">MAVLSSQVTPKRMPKFYRGNAACPGCPIPKELDVALEVLGNKTVLVVPASCTTIIMGDTNGMPSTVPVVHSAFGAAAAIGSGIVRSLRMRGDNDAIVAVWAGDGSTGDIGFAAVSGAAERNEDILYICYDNEAYMNTGIQRSGLTPKGAWTTTTPEGKREVKKPLPFIIAEHKVPYVATASIAYIYDYEAKMRKAKQIRGFRYIHLLSPCPPGWRFDSKLTIDIAKLAVETGVWPLFEIENGEFKLTSVSKTLVEKKNRKPVAEYLKLQGRFKQLTEEQIKGIQEEIDEMWEEIKRLIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z08</accession>
    <name>Q97Z08_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Na(+)/H(+) antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">SSO1138</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90266">
        <property type="entry name" value="G90266"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989706.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z08"/>
    <dbReference type="FunCoup" id="Q97Z08">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1138"/>
    <dbReference type="PaxDb" id="273057-SSO1138"/>
    <dbReference type="EnsemblBacteria" id="AAK41390">
        <property type="protein sequence ID" value="AAK41390"/>
        <property type="gene ID" value="SSO1138"/>
    </dbReference>
    <dbReference type="GeneID" id="1454169"/>
    <dbReference type="KEGG" id="sso:SSO1138"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1130"/>
    <dbReference type="eggNOG" id="arCOG01953">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005126_7_1_2"/>
    <dbReference type="InParanoid" id="Q97Z08"/>
    <dbReference type="PhylomeDB" id="Q97Z08"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562">
        <property type="entry name" value="NAPA-TYPE SODIUM/HYDROGEN ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562:SF3">
        <property type="entry name" value="SODIUM ION_PROTON EXCHANGER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="227"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="299"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="11">
        <location>
            <begin position="15"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK41390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="391" mass="41628" checksum="17693FE15070E2F4" modified="2001-10-01" version="1">MNPIILALFDVSIFILLAEILSSLVQKYGLPKLIGELLAGMIIGPYALGSLLNQLLGFPLIGINSYIEFLAEFSVILLIFASGLEHGIAPIKSSGILGFLGATFGALLPFFAAYYFYISSFGLDSALILGTAMGATSLAAVASIIEEGKLKGKGINFMVSSAAADDVVDLLLLSVVIAILQGESTSVTSISLKIIALVVIWVVILVVSIILIPKITDRISDKYIEEFPLAVLFGLTLLMVSLGYSPIISAFVAGVAFANSVKNSKIKEISSTLLSVFGPLFFVYIGAEVNFLTLNLNTVILSLELTAIATIFKWLGIFPFALAYLRNAKAANTVALGMVPRGETGLVIASLGLSYNALNQEEFEGIVFMSIFTTLIGSALFKIYAKKHLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z09</accession>
    <name>Q97Z09_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90266">
        <property type="entry name" value="F90266"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z09"/>
    <dbReference type="STRING" id="273057.SSO1137"/>
    <dbReference type="PaxDb" id="273057-SSO1137"/>
    <dbReference type="EnsemblBacteria" id="AAK41389">
        <property type="protein sequence ID" value="AAK41389"/>
        <property type="gene ID" value="SSO1137"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1137"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1129"/>
    <dbReference type="eggNOG" id="arCOG06017">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121284_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z09"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="171" mass="19950" checksum="A9E7612C4A2EC03E" modified="2001-10-01" version="1">MCEFKVVTIERPIREDNNTVLIDYYDFVKISDTKIMNVLVKDSRENYSKSYYYYIRDYLNKLRILKENMINVKLVFPFEKANGSLNLKKGIIYVTNDKQLVYMNLHSNVYANCENCIAKPFCTYYLAKIIGENRLKIGVNKGNPGESWDKALSSLQSKYVKTKVIELPPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z36</accession>
    <name>Q97Z36_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transport membrane protein (Permease)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1103</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99263">
        <property type="entry name" value="A99263"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989736.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z36"/>
    <dbReference type="FunCoup" id="Q97Z36">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1103"/>
    <dbReference type="PaxDb" id="273057-SSO1103"/>
    <dbReference type="EnsemblBacteria" id="AAK41360">
        <property type="protein sequence ID" value="AAK41360"/>
        <property type="gene ID" value="SSO1103"/>
    </dbReference>
    <dbReference type="GeneID" id="1454139"/>
    <dbReference type="KEGG" id="sso:SSO1103"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1100"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_5_5_2"/>
    <dbReference type="InParanoid" id="Q97Z36"/>
    <dbReference type="PhylomeDB" id="Q97Z36"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052952">
        <property type="entry name" value="MFS-Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23527">
        <property type="entry name" value="BLL3282 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23527:SF1">
        <property type="entry name" value="BLL3282 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="5"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="378" mass="42236" checksum="E97476C97466BCF5" modified="2001-10-01" version="1">MKAKRVIFLSASSFFLSYFSRVTWSIVAPLSTLKTTTTEDSIIFALFFLGYILVQIPSGMLADRISANHLLFLSLLGVAITSFISSTFPLIMLEYVASFLMGFSAGWIYPITVKLLSASFDSRDLPIAMSIYSISWPLSIVASGVIIPFLALTFGWEFSFYFITALSIILGILALLYLPSLKLSKSIIKFKDVVKDKNSIYISVGGFLFYLTYWILVLYLYKYLLSVVGNEYTAGIIYSFTALTGIFSTILAGYIIKSLGVKRTFLSFITLYSFSLLLFSFSKNVIVIGIDALALGFFRFVITPTSSTAVAVIGGKERSGSVTGLANFFWQFSGIVGSIIAPLLINLFTYTYLWTFVSVFSFLSLIFYYKLKFMRDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z42</accession>
    <name>Q97Z42_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Aconitate hydratase</fullName>
            <ecNumber evidence="8">4.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1095</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90262">
        <property type="entry name" value="C90262"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989728.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z42"/>
    <dbReference type="FunCoup" id="Q97Z42">
        <property type="interactions" value="366"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1095"/>
    <dbReference type="PaxDb" id="273057-SSO1095"/>
    <dbReference type="EnsemblBacteria" id="AAK41354">
        <property type="protein sequence ID" value="AAK41354"/>
        <property type="gene ID" value="SSO1095"/>
    </dbReference>
    <dbReference type="GeneID" id="1454133"/>
    <dbReference type="KEGG" id="sso:SSO1095"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1092"/>
    <dbReference type="eggNOG" id="arCOG01697">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013476_2_1_2"/>
    <dbReference type="InParanoid" id="Q97Z42"/>
    <dbReference type="PhylomeDB" id="Q97Z42"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030350">
        <property type="term" value="F:iron-responsive element binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01586">
        <property type="entry name" value="AcnA_IRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000011">
        <property type="entry name" value="Aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000025">
        <property type="entry name" value="Aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01341">
        <property type="entry name" value="aconitase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006757">
        <property type="entry name" value="PRK09277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009520">
        <property type="entry name" value="PRK12881.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
        <property type="entry name" value="ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="6">
        <location>
            <begin position="64"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="7">
        <location>
            <begin position="663"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="855" mass="95775" checksum="D9876DF9AA6BA84F" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z50</accession>
    <name>Q97Z50_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1086</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90261">
        <property type="entry name" value="C90261"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z50"/>
    <dbReference type="PaxDb" id="273057-SSO1086"/>
    <dbReference type="EnsemblBacteria" id="AAK41346">
        <property type="protein sequence ID" value="AAK41346"/>
        <property type="gene ID" value="SSO1086"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1086"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1084"/>
    <dbReference type="eggNOG" id="arCOG05972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2230493_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z50"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="106" mass="12442" checksum="B899F6DAF64048EA" modified="2001-10-01" version="1">MMVKVYKIGDYYIAGVEHVIQGYLQDVVFVYKNNNNWVSVSAERFRSNDPSINKVKEAVKYATHEEDLKKAIEELRSSGIKIEEVKEIPFPRKFIEGRKKIQEEFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z70</accession>
    <name>Q97Z70_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoate protein ligase A, hypothetical (LplA-like1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lplA-like1</name>
        <name evidence="2" type="ordered locus">SSO1060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90258">
        <property type="entry name" value="D90258"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z70"/>
    <dbReference type="FunCoup" id="Q97Z70">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1060"/>
    <dbReference type="PaxDb" id="273057-SSO1060"/>
    <dbReference type="EnsemblBacteria" id="AAK41323">
        <property type="protein sequence ID" value="AAK41323"/>
        <property type="gene ID" value="SSO1060"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1060"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1056"/>
    <dbReference type="eggNOG" id="arCOG01939">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022986_1_0_2"/>
    <dbReference type="InParanoid" id="Q97Z70"/>
    <dbReference type="PhylomeDB" id="Q97Z70"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033819">
        <property type="term" value="F:lipoyl(octanoyl) transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009107">
        <property type="term" value="P:lipoate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050664">
        <property type="entry name" value="Octanoyltrans_LipM/LipL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679:SF2">
        <property type="entry name" value="OCTANOYL-[GCVH]:PROTEIN N-OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679">
        <property type="entry name" value="OCTANOYLTRANSFERASE LIPM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="30"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="279" mass="31830" checksum="B77A4E232A58EB64" modified="2001-10-01" version="1">MSWRFVSLPPQDGYHMVTSFVSVADYVNRGGKNTLLVFSVKEPFVNVGIHQEVWLEVDLEFTKKMKIPVVRRDLGGGTVVITPGEHDYFIVVRQEDAPRNPSELYKKFLTPIVNVLRSYGLKADLRDQDIVVNGKKISGNGAMTHGNSIVIAGNILLSLDIDLISKCIRVPTEKFRDKMAKDMSEWLTSLERELGYIPPKEEINKKLKEAFEKELGIKFEDSTLTPEEIELWEKLASEKAKEEWIFYKDNRHRDIHTERCVKISSAALFATLTIRLGSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z94</accession>
    <name>Q97Z94_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90255">
        <property type="entry name" value="D90255"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z94"/>
    <dbReference type="STRING" id="273057.SSO1036"/>
    <dbReference type="PaxDb" id="273057-SSO1036"/>
    <dbReference type="EnsemblBacteria" id="AAK41299">
        <property type="protein sequence ID" value="AAK41299"/>
        <property type="gene ID" value="SSO1036"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1036"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1029"/>
    <dbReference type="eggNOG" id="arCOG08555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2313914_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z94"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="110" mass="11887" checksum="F9881EE374A9BF7B" modified="2001-10-01" version="1">MLLTTVLTEVVMPKLQITGEIGGIPPVNVVAANWGNVKVWAAAYYSIWFITSPETIGYYIAQPIPSNTNLIGQVDYGAAGAINLNITPWWARSVSVGSGYSYSYQTLSIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZB0</accession>
    <name>Q97ZB0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90253">
        <property type="entry name" value="D90253"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989222.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZB0"/>
    <dbReference type="STRING" id="273057.SSO1020"/>
    <dbReference type="PaxDb" id="273057-SSO1020"/>
    <dbReference type="EnsemblBacteria" id="AAK41283">
        <property type="protein sequence ID" value="AAK41283"/>
        <property type="gene ID" value="SSO1020"/>
    </dbReference>
    <dbReference type="GeneID" id="15297577"/>
    <dbReference type="KEGG" id="sso:SSO1020"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1011"/>
    <dbReference type="eggNOG" id="arCOG08457">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2243973_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZB0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="118" mass="14026" checksum="7570612665EDF817" modified="2001-10-01" version="1">MFKRKRTKGENRGEDKENRGTEEENRGNEKRPREDISTTIDHNRVYEEECSELLRQLLNLSSRESALTFELFQLSEEAKVETLRLAGLIANCKYYDKALAEELYKELDILFCKYLERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZD3</accession>
    <name>Q97ZD3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycogen synthase</fullName>
            <ecNumber evidence="5">2.4.1.21</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO0987</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.21" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90250">
        <property type="entry name" value="D90250"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989256.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZD3"/>
    <dbReference type="FunCoup" id="Q97ZD3">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0987"/>
    <dbReference type="CAZy" id="GT5">
        <property type="family name" value="Glycosyltransferase Family 5"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO0987"/>
    <dbReference type="EnsemblBacteria" id="AAK41259">
        <property type="protein sequence ID" value="AAK41259"/>
        <property type="gene ID" value="SSO0987"/>
    </dbReference>
    <dbReference type="GeneID" id="1455226"/>
    <dbReference type="KEGG" id="sso:SSO0987"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.984"/>
    <dbReference type="eggNOG" id="arCOG01420">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_18_5_2"/>
    <dbReference type="InParanoid" id="Q97ZD3"/>
    <dbReference type="PhylomeDB" id="Q97ZD3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009011">
        <property type="term" value="F:alpha-1,4-glucan glucosyltransferase (ADP-glucose donor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825:SF11">
        <property type="entry name" value="ALPHA AMYLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825">
        <property type="entry name" value="GRANULE-BOUND STARCH SYNTHASE 1, CHLOROPLASTIC/AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Starch synthase catalytic" evidence="4">
        <location>
            <begin position="15"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="3">
        <location>
            <begin position="345"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="566" mass="65596" checksum="16700049337E7806" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZK6</accession>
    <name>Q97ZK6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0901</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90240">
        <property type="entry name" value="G90240"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZK6"/>
    <dbReference type="STRING" id="273057.SSO0901"/>
    <dbReference type="PaxDb" id="273057-SSO0901"/>
    <dbReference type="EnsemblBacteria" id="AAK41182">
        <property type="protein sequence ID" value="AAK41182"/>
        <property type="gene ID" value="SSO0901"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0901"/>
    <dbReference type="eggNOG" id="arCOG07222">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176007_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZK6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="107" mass="12191" checksum="A6B49E4AF85EB88A" modified="2001-10-01" version="1">MRKMFEVGFKVLAEDPFRIKYLSQSINDVFKDLCEPVKIGASYICAPNQDTLLLIYFSSQLSKDMNASLKIMSNNATYVVEIMREVNSRLRSQGFYITISETSSTSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZK9</accession>
    <name>SYV_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Valine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Valyl-tRNA synthetase</fullName>
            <shortName evidence="1">ValRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">valS</name>
        <name type="ordered locus">SSO0899</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90240">
        <property type="entry name" value="D90240"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992319.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZK9"/>
    <dbReference type="SMR" id="Q97ZK9"/>
    <dbReference type="FunCoup" id="Q97ZK9">
        <property type="interactions" value="196"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0899"/>
    <dbReference type="PaxDb" id="273057-SSO0899"/>
    <dbReference type="EnsemblBacteria" id="AAK41179">
        <property type="protein sequence ID" value="AAK41179"/>
        <property type="gene ID" value="SSO0899"/>
    </dbReference>
    <dbReference type="GeneID" id="1455146"/>
    <dbReference type="KEGG" id="sso:SSO0899"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.900"/>
    <dbReference type="eggNOG" id="arCOG00808">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_0_2_2"/>
    <dbReference type="InParanoid" id="Q97ZK9"/>
    <dbReference type="PhylomeDB" id="Q97ZK9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00817">
        <property type="entry name" value="ValRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000033">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000192">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000324">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02005">
        <property type="entry name" value="Val_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022874">
        <property type="entry name" value="Valine-tRNA_ligase_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009687">
        <property type="entry name" value="PRK13208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF93">
        <property type="entry name" value="VALINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224635" description="Valine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="572"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="575"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02005"/>
        </source>
    </evidence>
    <sequence length="842" mass="98578" checksum="916108C2583B46D5" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZL8</accession>
    <name>Q97ZL8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">malonyl CoA reductase (malonate semialdehyde-forming)</fullName>
            <ecNumber evidence="5">1.2.1.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">asd-1</name>
        <name evidence="8" type="ordered locus">SSO0874</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>3-oxopropanoate + NADP(+) + CoA = malonyl-CoA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26446"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33190"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.75"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.75" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90238">
        <property type="entry name" value="C90238"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992293.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZL8"/>
    <dbReference type="FunCoup" id="Q97ZL8">
        <property type="interactions" value="236"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0874"/>
    <dbReference type="PaxDb" id="273057-SSO0874"/>
    <dbReference type="EnsemblBacteria" id="AAK41162">
        <property type="protein sequence ID" value="AAK41162"/>
        <property type="gene ID" value="SSO0874"/>
    </dbReference>
    <dbReference type="GeneID" id="1455129"/>
    <dbReference type="KEGG" id="sso:SSO0874"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.883"/>
    <dbReference type="eggNOG" id="arCOG00494">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049966_1_0_2"/>
    <dbReference type="InParanoid" id="Q97ZL8"/>
    <dbReference type="PhylomeDB" id="Q97ZL8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18130">
        <property type="entry name" value="ASADH_C_arch_fung_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02315">
        <property type="entry name" value="ScASADH_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000076">
        <property type="entry name" value="Aspartate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000411">
        <property type="entry name" value="Aspartate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051823">
        <property type="entry name" value="ASADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005676">
        <property type="entry name" value="Asp_semi-ald_DH_pep-lack"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00978">
        <property type="entry name" value="asd_EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006416">
        <property type="entry name" value="PRK08664.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718:SF1">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000148">
        <property type="entry name" value="ASA_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="7">
        <location>
            <begin position="6"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="6">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00052213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000148-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="350" mass="38692" checksum="B93ABD392519A4F2" modified="2001-10-01" version="1">MADKIKVSLLGSTGMVGQKMVRMLSKHPYIELVKVSASPQKIGKKYKDAVKWIEPGDIPENVAELPIVSTEYEDHKDVDVVLSALPNELAEDVELKLVKQGKIVVSNASPFRMDPDVPLINPEVNWEHLELLKYQKSSKNWNGLLVKNPNCTAAILSMPIKPLERLIKINATYIVTLQAVSGAGYSGLPFMAIDGNVVPWIKGEEEKIPKEINKMLGKFEAGKLKSSSLEIHPTTTRVPVKVGHMGVINIVTDDNVDEKEVQKALDSFASLPQHKNLPTAPNKPIIVFKDEDRPQPARDLQYYDGMAVTVGRVKFEGNVLRLVVLGDNLVRGAAGITILTLEVMKELGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZM5</accession>
    <name>Q97ZM5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0866</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90237">
        <property type="entry name" value="D90237"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992286.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZM5"/>
    <dbReference type="STRING" id="273057.SSO0866"/>
    <dbReference type="PaxDb" id="273057-SSO0866"/>
    <dbReference type="EnsemblBacteria" id="AAK41155">
        <property type="protein sequence ID" value="AAK41155"/>
        <property type="gene ID" value="SSO0866"/>
    </dbReference>
    <dbReference type="GeneID" id="7806727"/>
    <dbReference type="KEGG" id="sso:SSO0866"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.876"/>
    <dbReference type="eggNOG" id="arCOG04266">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162442_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZM5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="108" mass="12806" checksum="72CDA5BD0BB6FB4D" modified="2001-10-01" version="1">MSTNKSVKMSEDEINKALAKAEKEAEKKDHKKQWIERMIKSAKTYYKLCPYYDKKNTKCFLTLGDKCQRDGKYETCPIFISFLDNKYQEIVNKKKMLPMDFQDLALMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZN3</accession>
    <name>Q97ZN3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D212 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0852</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90236">
        <property type="entry name" value="C90236"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZN3"/>
    <dbReference type="STRING" id="273057.SSO0852"/>
    <dbReference type="PaxDb" id="273057-SSO0852"/>
    <dbReference type="EnsemblBacteria" id="AAK41146">
        <property type="protein sequence ID" value="AAK41146"/>
        <property type="gene ID" value="SSO0852"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0852"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.868"/>
    <dbReference type="HOGENOM" id="CLU_1773290_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZN3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022012">
        <property type="entry name" value="D212_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12187">
        <property type="entry name" value="VirArc_Nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D212 catalytic" evidence="1">
        <location>
            <begin position="24"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="146" mass="17066" checksum="411251539615C8FD" modified="2001-10-01" version="1">MHLGHSLERTDRTWEGDPFKKFDFYIPKYNLYLDVTGTSFTKSESIKRAHMLQLRGAIIAILGIKVSVAEILAAKGYRTAFANVAEREGEIRFMPYSRLKVLEKNGRAIIAQPEEIEFAKGERTYILTRWTDWLKPNQFLRWLKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZN5</accession>
    <name>Q97ZN5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycine zipper 2TM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0849</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90235">
        <property type="entry name" value="H90235"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923138.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZN5"/>
    <dbReference type="STRING" id="273057.SSO0849"/>
    <dbReference type="PaxDb" id="273057-SSO0849"/>
    <dbReference type="EnsemblBacteria" id="AAK41143">
        <property type="protein sequence ID" value="AAK41143"/>
        <property type="gene ID" value="SSO0849"/>
    </dbReference>
    <dbReference type="GeneID" id="1455110"/>
    <dbReference type="KEGG" id="sso:SSO0849"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.865"/>
    <dbReference type="eggNOG" id="arCOG07289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1891523_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZN5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008816">
        <property type="entry name" value="Gly_zipper_2TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05433">
        <property type="entry name" value="Rick_17kDa_Anti"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycine zipper 2TM" evidence="1">
        <location>
            <begin position="64"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="134" mass="14879" checksum="6223CA277C917674" modified="2001-10-01" version="1">MNEYIARYVVSKDHRIIGVINTRYTANNDDESIKIAKSVCEKLGEEDIEELIIGKVIGTVSVDVIGNLLGKSDDDKLLGTIAGAIVGTLVGHAIDSTITKVIYSKKYPCIKPRPFEDIVNYVDLERLEEIVNER</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZS5</accession>
    <name>Q97ZS5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Purine nucleosidase, putative (IunH-1)</fullName>
            <ecNumber evidence="4">3.2.2.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">iunH-1</name>
        <name evidence="4" type="ordered locus">SSO0505</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="Biochemistry" volume="51" first="4590" last="4599">
            <title>New determinants in the catalytic mechanism of nucleoside hydrolases from the structures of two isozymes from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Minici C."/>
                <person name="Cacciapuoti G."/>
                <person name="De Leo E."/>
                <person name="Porcelli M."/>
                <person name="Degano M."/>
            </authorList>
            <dbReference type="PubMed" id="22551416"/>
            <dbReference type="DOI" id="10.1021/bi300209g"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EC" id="3.2.2.1" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99196">
        <property type="entry name" value="A99196"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991400.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3T8J">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
        <property type="chains" value="A=1-311"/>
    </dbReference>
    <dbReference type="PDBsum" id="3T8J"/>
    <dbReference type="AlphaFoldDB" id="Q97ZS5"/>
    <dbReference type="SMR" id="Q97ZS5"/>
    <dbReference type="FunCoup" id="Q97ZS5">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0505"/>
    <dbReference type="PaxDb" id="273057-SSO0505"/>
    <dbReference type="EnsemblBacteria" id="AAK40824">
        <property type="protein sequence ID" value="AAK40824"/>
        <property type="gene ID" value="SSO0505"/>
    </dbReference>
    <dbReference type="GeneID" id="1455634"/>
    <dbReference type="KEGG" id="sso:SSO0505"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.499"/>
    <dbReference type="eggNOG" id="arCOG04558">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036838_2_1_2"/>
    <dbReference type="InParanoid" id="Q97ZS5"/>
    <dbReference type="PhylomeDB" id="Q97ZS5"/>
    <dbReference type="BRENDA" id="3.2.2.8">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97ZS5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008477">
        <property type="term" value="F:purine nucleosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006152">
        <property type="term" value="P:purine nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02649">
        <property type="entry name" value="nuc_hydro_CeIAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.245.10:FF:000024">
        <property type="entry name" value="Purine nucleosidase, putative (IunH-1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023186">
        <property type="entry name" value="IUNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304">
        <property type="entry name" value="INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304:SF4">
        <property type="entry name" value="URIDINE NUCLEOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="6" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="3">
        <location>
            <begin position="5"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="3T8J"/>
        </source>
    </evidence>
    <sequence length="311" mass="35212" checksum="1BD61FF2C3042DD9" modified="2001-10-01" version="1">MRHFIIDCDTAEDDVLSLYLLLKNNIDVVAVTIVEGNISYEQEVKNALWALEQVNREIPVYPGANKPLLKNYITVEKVHGKGGIGDVTVEPKRLKAQEKHAALAIIDLANEYAGELEFLAISPLTNLALAYLLDNSIVKKIKKVWVMGGAVFGIGNITPVAEFNIWVDPDAAKIVFNAGFDITMIPWDVIINYPVTDEEWNVIKNMKTRMSELYVSMYLHYRQYSSTVQKINGHPHPDAITTAIAIDGSIATRREKRFVVIDNTDNITRGMTLVDRFDADTSWSDKPNAEIVYEINKKSFMEKIYDLLNWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZS9</accession>
    <name>Q97ZS9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90195">
        <property type="entry name" value="D90195"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991390.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZS9"/>
    <dbReference type="PaxDb" id="273057-SSO0500"/>
    <dbReference type="EnsemblBacteria" id="AAK40819">
        <property type="protein sequence ID" value="AAK40819"/>
        <property type="gene ID" value="SSO0500"/>
    </dbReference>
    <dbReference type="GeneID" id="1455629"/>
    <dbReference type="KEGG" id="sso:SSO0500"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.493"/>
    <dbReference type="eggNOG" id="arCOG07280">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1248330_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZS9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="221" mass="25652" checksum="2C8AB0832F05F011" modified="2001-10-01" version="1">MYTILAYTDTIVFNVIRKAAYENFCTVYTIRSYSPSKLVASVGNIMIIVSRNNKSATISVKCGNAKKSFYIKVNENRINFDGNEMDTNLFIYHISSIENELYEYVKIISEKCNMQEICHKQKKGIKEILVEGKKINIGEEIKHSLEQLLTILYKREVSVECSKSSLCIKKVILTRRKVYIQLVDSEKENYWYLELNDLINKMPEHAQEILNIEGQIRAQSI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZW3</accession>
    <name>KAD6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative adenylate kinase</fullName>
            <shortName evidence="1">AK</shortName>
            <ecNumber evidence="1">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0461</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Also has ATPase activity. Involved in the late maturation steps of the 30S ribosomal particles, specifically 16S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with uS11. Not a structural component of 40S pre-ribosomes, but transiently interacts with them by binding to uS11.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90191">
        <property type="entry name" value="B90191"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922979.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZW3"/>
    <dbReference type="SMR" id="Q97ZW3"/>
    <dbReference type="FunCoup" id="Q97ZW3">
        <property type="interactions" value="218"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0461"/>
    <dbReference type="PaxDb" id="273057-SSO0461"/>
    <dbReference type="EnsemblBacteria" id="AAK40785">
        <property type="protein sequence ID" value="AAK40785"/>
        <property type="gene ID" value="SSO0461"/>
    </dbReference>
    <dbReference type="GeneID" id="84061998"/>
    <dbReference type="KEGG" id="sso:SSO0461"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.455"/>
    <dbReference type="eggNOG" id="arCOG01038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079096_3_1_2"/>
    <dbReference type="InParanoid" id="Q97ZW3"/>
    <dbReference type="PhylomeDB" id="Q97ZW3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595:SF0">
        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000153915" description="Putative adenylate kinase">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMP" evidence="1">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="1">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00039"/>
        </source>
    </evidence>
    <sequence length="187" mass="21247" checksum="8EC5517CA85D5F22" modified="2001-10-01" version="1">MIIIVTGTPGVGKTVASKKLSEALNLNYLSLSQFVIENKLYTEYDELRQSYIIDEDKVKEELEKIISTSHLVIETIYPSLVSTADLVVVLRKNPFSLYNELKGRGWADIKVAENVEAEILGVISQEAREAFKDKVCEVDTTEMSTEQILNKILNKQCDGPIEWLVDTKVQRFLEELDKIISSYENDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZY0</accession>
    <name>Q97ZY0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0442</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90188">
        <property type="entry name" value="H90188"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988728.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZY0"/>
    <dbReference type="STRING" id="273057.SSO0442"/>
    <dbReference type="PaxDb" id="273057-SSO0442"/>
    <dbReference type="EnsemblBacteria" id="AAK40767">
        <property type="protein sequence ID" value="AAK40767"/>
        <property type="gene ID" value="SSO0442"/>
    </dbReference>
    <dbReference type="GeneID" id="1455577"/>
    <dbReference type="KEGG" id="sso:SSO0442"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.435"/>
    <dbReference type="eggNOG" id="arCOG04224">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157709_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZY0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="121" mass="13436" checksum="A152F53FBCA8B683" modified="2001-10-01" version="1">MIVMSSNENEKEEEKKVDIKTLVSVIPPASALKGNKAPPKEKRIKIRKRNDVDKGFAIISSKLAKDLGITDNIEISVKGKRIRLKAILQEGIPELEVWANPQDLVTLGIEDNSTVTARAYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZZ7</accession>
    <name>Q97ZZ7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0422</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90186">
        <property type="entry name" value="E90186"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZZ7"/>
    <dbReference type="FunCoup" id="Q97ZZ7">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0422"/>
    <dbReference type="PaxDb" id="273057-SSO0422"/>
    <dbReference type="EnsemblBacteria" id="AAK40748">
        <property type="protein sequence ID" value="AAK40748"/>
        <property type="gene ID" value="SSO0422"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0422"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.415"/>
    <dbReference type="eggNOG" id="arCOG00062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_3_1_2"/>
    <dbReference type="InParanoid" id="Q97ZZ7"/>
    <dbReference type="PhylomeDB" id="Q97ZZ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07197">
        <property type="entry name" value="nitrilase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF27">
        <property type="entry name" value="DEAMINATED GLUTATHIONE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="293" mass="32357" checksum="EC66C61803281590" modified="2001-10-01" version="1">MVKKFMLIALVHLRLKELSRKHNLEKAKKLIRQAKDKGAKLVILPSLFPSGNMFEIYDNDKKLRSFIKNLAEKIPGNNTDTLINLAMDGEVHVIVGPILEQAGPKIFLTSLIISPQGEIIGKYRKTVLSEKDIRLGISAGKEPVNVVLDRKYGIIAEDDIFSPEISRLLAMGGSEIVIGTMKALGKEQQVIKHLAIARTIENGIPYLIVGESIEDEEGEIIGYSPTFITSPDNLISKEAEEDDSLLFVESSILSSSKQTSLTYLEPIINGLCKGVKKIKGEAKKRSASTMEEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q980A9</accession>
    <name>METE_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methionine synthase</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Homocysteine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">metE</name>
        <name type="ordered locus">SSO0407</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a methyl group to L-homocysteine resulting in methionine formation. The physiological methyl donor is unknown.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the archaeal MetE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99185">
        <property type="entry name" value="A99185"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988777.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980A9"/>
    <dbReference type="SMR" id="Q980A9"/>
    <dbReference type="FunCoup" id="Q980A9">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0407"/>
    <dbReference type="PaxDb" id="273057-SSO0407"/>
    <dbReference type="EnsemblBacteria" id="AAK40736">
        <property type="protein sequence ID" value="AAK40736"/>
        <property type="gene ID" value="SSO0407"/>
    </dbReference>
    <dbReference type="GeneID" id="1455546"/>
    <dbReference type="KEGG" id="sso:SSO0407"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.403"/>
    <dbReference type="eggNOG" id="arCOG01876">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040013_3_2_2"/>
    <dbReference type="InParanoid" id="Q980A9"/>
    <dbReference type="PhylomeDB" id="Q980A9"/>
    <dbReference type="UniPathway" id="UPA00051"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00288">
        <property type="entry name" value="MetE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022921">
        <property type="entry name" value="MetE_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003317">
        <property type="entry name" value="PRK04326.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30519">
        <property type="entry name" value="5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000098692" description="Methionine synthase">
        <location>
            <begin position="1"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="332" mass="38250" checksum="88D4CF48846B1C2B" modified="2001-10-01" version="1">MSKLPLLPTTVIGSYPRPKWLRESIRLHKAGKMSNEDLQEAFNDAVIAVFQDHYKAGVDVPTDGEVRRDEMVEFFAERIKGFKFYGPVRVWGTAYYRKPSVVSKIEYREPMLVDEFTFAKSVSYTDNLKITITGPYTIAEWSYNEYYRNKKDLVFDLAKAINQEIKNLVEAGAKIIQIDEPALHTRKEDVSWGVEAVNEAVKGVNAKLVMHICYGDYSFVAPYFNEIKVDQINFALKIYNYKPLELLKKYGFDKELGAGVVDVHNRKVETSEEVANDIRKILEYFPPEKVWINPDCGLKLLSRKIAYQKLVSMVEGTKVVREELKRKGYSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q980B4</accession>
    <name>Q980B4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8 9">Riboflavin synthase</fullName>
            <ecNumber evidence="4 8 9">2.5.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ribC</name>
        <name evidence="10" type="ordered locus">SSO0401</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>2 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) = 5-amino-6-(D-ribitylamino)uracil + riboflavin</text>
            <dbReference type="Rhea" id="RHEA:20772"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="EC" id="2.5.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.9" evidence="4 8 9"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90184">
        <property type="entry name" value="C90184"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988788.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980B4"/>
    <dbReference type="FunCoup" id="Q980B4">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0401"/>
    <dbReference type="PaxDb" id="273057-SSO0401"/>
    <dbReference type="EnsemblBacteria" id="AAK40730">
        <property type="protein sequence ID" value="AAK40730"/>
        <property type="gene ID" value="SSO0401"/>
    </dbReference>
    <dbReference type="GeneID" id="1455540"/>
    <dbReference type="GeneID" id="44129382"/>
    <dbReference type="KEGG" id="sso:SSO0401"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.397"/>
    <dbReference type="eggNOG" id="arCOG01322">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1682776_0_0_2"/>
    <dbReference type="InParanoid" id="Q980B4"/>
    <dbReference type="PhylomeDB" id="Q980B4"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00405"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004746">
        <property type="term" value="F:riboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09210">
        <property type="entry name" value="Riboflavin_synthase_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="entry name" value="Lumazine/riboflavin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006399">
        <property type="entry name" value="Ribfl_synth_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01506">
        <property type="entry name" value="ribC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015750">
        <property type="entry name" value="Ribfl_synth_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="Lumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="7 9 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR01506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK40730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="155" mass="17106" checksum="D3BB9131114C4229" modified="2001-10-01" version="1">MTRKYGIADTTFSRVDMGSLVYKVIRSEDSEADIVRYTVPGIKDLPVAAKRLIDEGCDGVITLGWVGKTMLDKYSYLATSIGLIMVQILTSKHVIDVTVHEDEADDEEKLKEIAIDRATKHAKNLVKLVKEGKNALTKYAGKGLRQGYSNAGEID</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2024-05-29" modified="2025-06-18" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q980D0</accession>
    <name>RNJL_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNase aCSPSF2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Sso-RNase J</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0386</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="RNA" volume="17" first="99" last="107">
            <title>Identification of an RNase J ortholog in Sulfolobus solfataricus: implications for 5'-to-3' directional decay and 5'-end protection of mRNA in Crenarchaeota.</title>
            <authorList>
                <person name="Hasenoehrl D."/>
                <person name="Konrat R."/>
                <person name="Blaesi U."/>
            </authorList>
            <dbReference type="PubMed" id="21115637"/>
            <dbReference type="DOI" id="10.1261/rna.2418211"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2013" name="Nucleic Acids Res." volume="41" first="1091" last="1103">
            <title>Archaeal beta-CASP ribonucleases of the aCPSF1 family are orthologs of the eukaryal CPSF-73 factor.</title>
            <authorList>
                <person name="Phung D.K."/>
                <person name="Rinaldi D."/>
                <person name="Langendijk-Genevaux P.S."/>
                <person name="Quentin Y."/>
                <person name="Carpousis A.J."/>
                <person name="Clouet-d'Orval B."/>
            </authorList>
            <dbReference type="PubMed" id="23222134"/>
            <dbReference type="DOI" id="10.1093/nar/gks1237"/>
        </citation>
        <scope>NOMENCLATURE</scope>
    </reference>
    <comment type="function">
        <text evidence="2">A 5'-3' exoribonuclease, more active on 5'-monophosphorylated and 5'-hydroxylated RNA than 5'-tri-phosphorylated RNA; note there is no evidence for accumulation of 5'-monophosphorylated RNA in this organism (PubMed:21115637). Translation initiation factor 2 subunit gamma but not subunit alpha protects 5'-tri-phosphorylated RNA from degradation by this enzyme (PubMed:21115637).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90182">
        <property type="entry name" value="B90182"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980D0"/>
    <dbReference type="SMR" id="Q980D0"/>
    <dbReference type="STRING" id="273057.SSO0386"/>
    <dbReference type="PaxDb" id="273057-SSO0386"/>
    <dbReference type="EnsemblBacteria" id="AAK40713">
        <property type="protein sequence ID" value="AAK40713"/>
        <property type="gene ID" value="SSO0386"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0386"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.379"/>
    <dbReference type="eggNOG" id="arCOG00541">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009673_5_1_2"/>
    <dbReference type="InParanoid" id="Q980D0"/>
    <dbReference type="PhylomeDB" id="Q980D0"/>
    <dbReference type="BRENDA" id="3.1.13.B1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16295">
        <property type="entry name" value="TTHA0252-CPSF-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF52">
        <property type="entry name" value="MRNA 3-END PROCESSING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000460374" description="RNase aCSPSF2">
        <location>
            <begin position="1"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9V076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="21115637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="21115637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="23222134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="492" mass="54944" checksum="E9EB3B86579B6B08" modified="2001-10-01" version="1">MPMTFVFITVGFSITIFFSLVSIITLVAFICSVFIPILSIFFRSEGFIFAITCFIEEEVNLIVCGYDISQMNYFLKILGGGREVGRSAIEVGNSDGSIILDYGVNFDEKDNPNFPLQEMPGKVKGFVVSHAHLDHIGALPIYQIGSLNTKVYGTVATRIITETMLKDFLKLSGAKIPYEWVEVRKTMDNFMAIGYGEEVEIDSLKVSLYNAGHIPGSSIIKVSSEKGVIAFTGDINLTETKLMKPAEIENIGDANVLVMESTYGKFNHPNRKDVENDFYDKVMEVVESGGTVLVPAFSLARSQEVLSVLAERNFPYPVYYDGMSREITEIMLGFKEFLNRPDLLKKAYDNFNYVKGWEDRHRAWKEKGVIVASAGMLKGGPAVYYFKKLSENSKNAVFLVSYQAINTPGRKLLEMGKFDEYSGLLKARLEIFDFSSHAGRRQLLEIVKSVKDLEKVVLVHGSPDNESSLADLIKQEIGVEVITPENGQEISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q980G3</accession>
    <name>Q980G3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90176">
        <property type="entry name" value="F90176"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980G3"/>
    <dbReference type="STRING" id="273057.SSO0337"/>
    <dbReference type="PaxDb" id="273057-SSO0337"/>
    <dbReference type="EnsemblBacteria" id="AAK40669">
        <property type="protein sequence ID" value="AAK40669"/>
        <property type="gene ID" value="SSO0337"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0337"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.330"/>
    <dbReference type="eggNOG" id="arCOG05344">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_800770_0_0_2"/>
    <dbReference type="InParanoid" id="Q980G3"/>
    <dbReference type="PhylomeDB" id="Q980G3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="38369" checksum="D5D4E255F85B4A91" modified="2001-10-01" version="1">MIEMLGIKIPLRKTTILPVLSFTLSSYFLTNFIFYLQYTDYTGLVEFLLIGAPFIGRAITPITYPFLMSRINIERITYLSLGNMAILDILEFFMGTDTLALILLRVATGILFGLATSAAVELATQSRSKIIMGMTMGGWALGWILSALMSFLMGKMVLITSSFSLVFFLLIKRSNIDKKLFSNSGSISIAFSWKALLIFLLGFEPAYILQLIPSLLGENNAIEETIIAYSISFIAYIILPSIGNIRISSLLTSLMIGILGFTSFITLKAWMFIPFTVLGLGLNSLLPIIIKLMKVEVTKIGPSMNLASLSGFLIPSLVSIGDIEINSAILTLISSISLGAIVYKNLKITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q980J2</accession>
    <name>Q980J2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="5">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tkt-2</name>
        <name evidence="9" type="ordered locus">SSO0299</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90172">
        <property type="entry name" value="H90172"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990593.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980J2"/>
    <dbReference type="FunCoup" id="Q980J2">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0299"/>
    <dbReference type="PaxDb" id="273057-SSO0299"/>
    <dbReference type="EnsemblBacteria" id="AAK40639">
        <property type="protein sequence ID" value="AAK40639"/>
        <property type="gene ID" value="SSO0299"/>
    </dbReference>
    <dbReference type="GeneID" id="1455449"/>
    <dbReference type="KEGG" id="sso:SSO0299"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.295"/>
    <dbReference type="eggNOG" id="arCOG01051">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_1_1_2"/>
    <dbReference type="InParanoid" id="Q980J2"/>
    <dbReference type="PhylomeDB" id="Q980J2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07033">
        <property type="entry name" value="TPP_PYR_DXS_TK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000129">
        <property type="entry name" value="Transketolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051157">
        <property type="entry name" value="PDH/Transketolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43825">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43825:SF1">
        <property type="entry name" value="TRANSKETOLASE-LIKE PYRIMIDINE-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="8">
        <location>
            <begin position="7"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK40639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="313" mass="35086" checksum="6FC6ABD1EED323E3" modified="2001-10-01" version="1">MMQGNIYSMRETFGRLLADLGDKNKDLVVITADVGDSTRALYFREKFKDRYFNVGIAEQDMVNFAAGLAAVGKKPAIVNFGMFLMRAWEQIRNSIARMNLDVKMFVTHTGYSDHGDGSSHQVLEDIALMRVLPNMKVVVPADPKDIERSLPVIINEERGPLYYRIGREYSPPITIGQEYEFKIGKAYVIKDGSDLAIIGAGVVLWDALKAAEELEKLGISVAVINLFSIKPIDENTIEYYARKAGKIITIEEHSIYGGIGSAVAEVTARRYPVPIRFVGATTFGRSARSQRDLLDYYNINYKTIIREAIDLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q980J4</accession>
    <name>Q980J4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thiamine biosynthesis protein related protein (ThiF)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">thiF</name>
        <name evidence="2" type="ordered locus">SSO0298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90172">
        <property type="entry name" value="F90172"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980J4"/>
    <dbReference type="FunCoup" id="Q980J4">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0298"/>
    <dbReference type="PaxDb" id="273057-SSO0298"/>
    <dbReference type="EnsemblBacteria" id="AAK40637">
        <property type="protein sequence ID" value="AAK40637"/>
        <property type="gene ID" value="SSO0298"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0298"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.293"/>
    <dbReference type="eggNOG" id="arCOG01676">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_10_1_2"/>
    <dbReference type="InParanoid" id="Q980J4"/>
    <dbReference type="PhylomeDB" id="Q980J4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00757">
        <property type="entry name" value="ThiF_MoeB_HesA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001089">
        <property type="entry name" value="Thiamine biosynthesis protein ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF102">
        <property type="entry name" value="ADENYLYLTRANSFERASE AND SULFURTRANSFERASE MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="1">
        <location>
            <begin position="46"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="333" mass="37279" checksum="AD25E4BF337F744D" modified="2001-10-01" version="1">MSNSSIDIGVPSLPPPFSIGTISIPTFSRQHLFLKIILHYNLVERYSRQLIVLGLGIQQRLNELKILIAGCGALGTAVAELLARLGVKELTIVDADVVDITNLHRVHLFDENDVGKPKAEVCAKKISLINSSIKINYIIDILDEENVERLISDKDYVFDALDSLYYKLLLNDAIVKLGKILIYGGINGEYGSAKLIDPSQTSCLSCFIDYSDQDEIGNSCDVIGTTPLIVELTATLQVNLMLNHLRGNPDYSLFYIDSRELKIERINIEKNSQCKTCVLNEYPFLYQKISKPKCGIFRTNMKIPELDEPKVFKNLGNVTLCYPSIGCFEKRGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-20" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q980W2</accession>
    <name>SRP19_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Signal recognition particle 19 kDa protein</fullName>
            <shortName evidence="1">SRP19</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">srp19</name>
        <name type="ordered locus">SSO0165</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SRP19 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90156">
        <property type="entry name" value="G90156"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990389.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3KTW">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.20 A"/>
        <property type="chains" value="A/B=2-102"/>
    </dbReference>
    <dbReference type="PDBsum" id="3KTW"/>
    <dbReference type="AlphaFoldDB" id="Q980W2"/>
    <dbReference type="SMR" id="Q980W2"/>
    <dbReference type="STRING" id="273057.SSO0165"/>
    <dbReference type="PaxDb" id="273057-SSO0165"/>
    <dbReference type="EnsemblBacteria" id="AAK40510">
        <property type="protein sequence ID" value="AAK40510"/>
        <property type="gene ID" value="SSO0165"/>
    </dbReference>
    <dbReference type="GeneID" id="1453337"/>
    <dbReference type="KEGG" id="sso:SSO0165"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.161"/>
    <dbReference type="eggNOG" id="arCOG01217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169299_1_0_2"/>
    <dbReference type="InParanoid" id="Q980W2"/>
    <dbReference type="EvolutionaryTrace" id="Q980W2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.30">
        <property type="entry name" value="Signal recognition particle, SRP19-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00305">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002778">
        <property type="entry name" value="Signal_recog_particle_SRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036521">
        <property type="entry name" value="SRP19-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022938">
        <property type="entry name" value="SRP19_arc-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01922">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69695">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0733">Signal recognition particle</keyword>
    <feature type="chain" id="PRO_0000135225" description="Signal recognition particle 19 kDa protein">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="5"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="11"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="17"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="24"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="54"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="70"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="76"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00305"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="3KTW"/>
        </source>
    </evidence>
    <sequence length="102" mass="12029" checksum="7AD3F7178B42AA40" modified="2001-10-01" version="1">MSLRDLKEENRIVIWPSYFFSPTRSKGRRLARIPYKIKTEELVSTLRELGLDPIVIENKKYPRDRKINFLIAVKKVKSKNYTLKIIHNALMGTRQTNPNKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q981A9</accession>
    <name>Q981A9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator PadR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90143">
        <property type="entry name" value="D90143"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989599.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981A9"/>
    <dbReference type="STRING" id="273057.SSO0038"/>
    <dbReference type="PaxDb" id="273057-SSO0038"/>
    <dbReference type="EnsemblBacteria" id="AAK40403">
        <property type="protein sequence ID" value="AAK40403"/>
        <property type="gene ID" value="SSO0038"/>
    </dbReference>
    <dbReference type="GeneID" id="1455294"/>
    <dbReference type="KEGG" id="sso:SSO0038"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.39"/>
    <dbReference type="eggNOG" id="arCOG00002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_1_3_2"/>
    <dbReference type="InParanoid" id="Q981A9"/>
    <dbReference type="PhylomeDB" id="Q981A9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252:SF2">
        <property type="entry name" value="TRANSCRIPTION REGULATOR, PADR-LIKE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR YQJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="19"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="148" mass="17522" checksum="03D3A2846EDEBDEA" modified="2001-10-01" version="1">MISKFSFQRLKKGALKYLVLESLNERPMRTYEIIKTIEKKFEGSYRPSTGSVYPVLKSLVEKNLVEVKVENNKKIYIITEKGKNELQEIKNKSLKLLGDNTKFSRQILQELLQIAFYLYDNRSKIDENNIQKIIEYLRNCRNQLKNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q981C0</accession>
    <name>Q981C0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90142">
        <property type="entry name" value="A90142"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989614.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981C0"/>
    <dbReference type="STRING" id="273057.SSO0025"/>
    <dbReference type="PaxDb" id="273057-SSO0025"/>
    <dbReference type="EnsemblBacteria" id="AAK40392">
        <property type="protein sequence ID" value="AAK40392"/>
        <property type="gene ID" value="SSO0025"/>
    </dbReference>
    <dbReference type="GeneID" id="1455283"/>
    <dbReference type="KEGG" id="sso:SSO0025"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.28"/>
    <dbReference type="eggNOG" id="arCOG05905">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1335106_0_0_2"/>
    <dbReference type="InParanoid" id="Q981C0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="205" mass="24261" checksum="EC01D31020990FA2" modified="2001-10-01" version="1">MQLYKYSLKDGYLVPNENGDVTVFVEGNFISITDKNGNKIEGARFKYLGNEYLLLEKLRYLAKLVNVDVDEDVLLAHPTLRNRTLAINKLMGELFEVFIYNLLLAKDYKVKRQFEIYPSLRKFTHTRWHNRPDFIVEDKLVIEAKIHKNDYLQTLEYSKYFKYGMAVFPFTGECRVPKGWICIFNTTKDQSRFYSLLEDLLSRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q981C6</accession>
    <name>Q981C6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdenum cofactor biosynthesis protein B (MoaB)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">moaB</name>
        <name evidence="2" type="ordered locus">SSO0019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90141">
        <property type="entry name" value="C90141"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981C6"/>
    <dbReference type="FunCoup" id="Q981C6">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0019"/>
    <dbReference type="PaxDb" id="273057-SSO0019"/>
    <dbReference type="EnsemblBacteria" id="AAK40386">
        <property type="protein sequence ID" value="AAK40386"/>
        <property type="gene ID" value="SSO0019"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0019"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.21"/>
    <dbReference type="eggNOG" id="arCOG00214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077358_2_3_2"/>
    <dbReference type="InParanoid" id="Q981C6"/>
    <dbReference type="PhylomeDB" id="Q981C6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.980.10:FF:000006">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012245">
        <property type="entry name" value="MoaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232:SF2">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006443">
        <property type="entry name" value="MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="1">
        <location>
            <begin position="22"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="182" mass="20582" checksum="70F74FEF5C7FA89C" modified="2001-10-01" version="1">MNNLMSAHKQHRQHAPTNINFYVITISTSRYEKLVRKEPLIDESGDIIKQLIIQSNYNIIGYELVPDEKVKILKAFANALDNSQTDVIISTGGTGYTQSDVTVETIRRIFDREIEGFSDVFRLVSYNDPEVKSASYLTKATAGIINDKVIFLLPGSPDAVRLAMKELILPEIGHLLYIVRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q981D7</accession>
    <name>Q981D7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">X-pro aminopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0010</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90139">
        <property type="entry name" value="H90139"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989641.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981D7"/>
    <dbReference type="FunCoup" id="Q981D7">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0010"/>
    <dbReference type="PaxDb" id="273057-SSO0010"/>
    <dbReference type="EnsemblBacteria" id="AAK40375">
        <property type="protein sequence ID" value="AAK40375"/>
        <property type="gene ID" value="SSO0010"/>
    </dbReference>
    <dbReference type="GeneID" id="1455265"/>
    <dbReference type="KEGG" id="sso:SSO0010"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.10"/>
    <dbReference type="eggNOG" id="arCOG01000">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_4_1_2"/>
    <dbReference type="InParanoid" id="Q981D7"/>
    <dbReference type="PhylomeDB" id="Q981D7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01092">
        <property type="entry name" value="APP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050659">
        <property type="entry name" value="Peptidase_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112:SF9">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="5">
        <location>
            <begin position="3"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="4">
        <location>
            <begin position="132"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="351" mass="40505" checksum="BB63B323F15E5EFC" modified="2001-10-01" version="1">MNRIQRVQKELERANADYLITGTTSNMYYLIGFSEEQMERPLLLFITRDDYFLLVPKLYEEQLKQFPLIVYRDGEDPYSKLNLKENSNVLIDDTTFSLFTIEILNRFKPGRIGRASRILGKLRQVKDDEELEKMEKGVKKAEQLILEFVSNIKENMTECQIERKLKSFLIEEAGNISFDPIVTSGPNSSMPHLRCSDKKVKRGEAIVIDYGIKHDGYSTDTTRVFSLGKPNDPLILEIVEIVKTANEEAEKHVREGMRAKEIDYFAREVITNKGYGDYFIHRTGHGIGIDVHEDPYISPDNDDVIEQNMVFTIEPGIYLPGKFGIRIEDEVVVKKGYGKTLNMLQKELYII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXA6</accession>
    <name>RL4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl4</name>
        <name type="ordered locus">SSO0718</name>
        <name type="ORF">C10_013</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99220">
        <property type="entry name" value="B99220"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991281.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXA6"/>
    <dbReference type="SMR" id="Q9UXA6"/>
    <dbReference type="FunCoup" id="Q9UXA6">
        <property type="interactions" value="235"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0718"/>
    <dbReference type="PaxDb" id="273057-SSO0718"/>
    <dbReference type="EnsemblBacteria" id="AAK41017">
        <property type="protein sequence ID" value="AAK41017"/>
        <property type="gene ID" value="SSO0718"/>
    </dbReference>
    <dbReference type="GeneID" id="1454984"/>
    <dbReference type="KEGG" id="sso:SSO0718"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.717"/>
    <dbReference type="eggNOG" id="arCOG04071">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026535_0_0_2"/>
    <dbReference type="InParanoid" id="Q9UXA6"/>
    <dbReference type="PhylomeDB" id="Q9UXA6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1370.10:FF:000011">
        <property type="entry name" value="50S ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_A">
        <property type="entry name" value="Ribosomal_uL4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013000">
        <property type="entry name" value="Ribosomal_uL4_euk/arc_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019970">
        <property type="entry name" value="Ribosomall_uL4-arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03672">
        <property type="entry name" value="rpl4p_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00939">
        <property type="entry name" value="RIBOSOMAL_L1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000129344" description="Large ribosomal subunit protein uL4">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="267" mass="29534" checksum="53CA92332CE50FD2" modified="2000-05-01" version="1">MYLELVKKKSDILDKDGNRIKEVELPIIFSFPVRKDLIRRVFIAEFTHSLQPKGRDPNAGKRTSAESFGINLGMARVPRVKNSGEAALAPNTVGGRLAFPPSVNKKLAEEANVKEKRLAVISALSATADIAFVRARGHVFKDSVRFPIVVTDDIVNLKTTSEVEEFLKKIGVYDDVERVKERIRIRAGKGKMRGRKYKEPIGPLIIVHDSNSPIIKAARNLAGVDVVNAKDVSVIHLAPGAHPGRLTIYTESSIKILDERLSKRVVS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>O33773</accession>
    <name>HIS7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Imidazoleglycerol-phosphate dehydratase</fullName>
            <shortName>IGPD</shortName>
            <ecNumber>4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hisB</name>
        <name type="ordered locus">SSO0596</name>
        <name type="ORF">C08_054</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="J. Bacteriol." volume="179" first="4429" last="4432">
            <title>Evolutionary analysis of the hisCGABdFDEHI gene cluster from the archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Sensen C.W."/>
                <person name="Doolittle W.F."/>
                <person name="Brown J.R."/>
            </authorList>
            <dbReference type="PubMed" id="9209067"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19"/>
    <dbReference type="EMBL" id="U82227">
        <property type="protein sequence ID" value="AAB63021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90206">
        <property type="entry name" value="D90206"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991115.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O33773"/>
    <dbReference type="SMR" id="O33773"/>
    <dbReference type="FunCoup" id="O33773">
        <property type="interactions" value="114"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0596"/>
    <dbReference type="PaxDb" id="273057-SSO0596"/>
    <dbReference type="EnsemblBacteria" id="AAK40907">
        <property type="protein sequence ID" value="AAK40907"/>
        <property type="gene ID" value="SSO0596"/>
    </dbReference>
    <dbReference type="GeneID" id="1454874"/>
    <dbReference type="KEGG" id="sso:SSO0596"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.603"/>
    <dbReference type="eggNOG" id="arCOG04398">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044308_3_0_2"/>
    <dbReference type="InParanoid" id="O33773"/>
    <dbReference type="PhylomeDB" id="O33773"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000001">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000003">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="entry name" value="Imidazole glycerol phosphate dehydratase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002114">
        <property type="entry name" value="PRK00951.2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010121">
        <property type="entry name" value="PRK13598.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133:SF2">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000158197" description="Imidazoleglycerol-phosphate dehydratase">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="193" mass="21437" checksum="DB0C7E108F08B6FB" modified="1998-01-01" version="1">MSRSANITRETKETKIEVLLDIDRKGEVKVSTPIPFFNHMLITLLTYMNSTAIVSATDKLPYDDHHIVEDVAITLGLAIKTALGDKRGIKRFSHQIIPMDDALVLVSLDISNRGMAFVNLNLKRSEIGGLATENVPHFFQSFAYNSGITLHISQLSGYNTHHIIEASFKALGLALYEATRIVDNEIRSTKGII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="142" xmlns="https://uniprot.org/uniprot">
    <accession>P95949</accession>
    <name>LHRC_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>ATP-dependent helicase Lhr-Core</fullName>
            <ecNumber evidence="4">5.6.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA 3'-5' helicase Lhr-Core</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Hel112</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0112</name>
        <name type="ORF">C04_010</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2007" name="Biochem. J." volume="408" first="87" last="95">
            <title>A novel DNA helicase with strand-annealing activity from the crenarchaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="De Felice M."/>
                <person name="Aria V."/>
                <person name="Esposito L."/>
                <person name="De Falco M."/>
                <person name="Pucci B."/>
                <person name="Rossi M."/>
                <person name="Pisani F.M."/>
            </authorList>
            <dbReference type="PubMed" id="17683280"/>
            <dbReference type="DOI" id="10.1042/bj20070134"/>
        </citation>
        <scope>FUNCTION AS A HELICASE</scope>
        <scope>FUNCTION AS AN ATPASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
        <scope>DNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2012" name="J. Biol. Chem." volume="287" first="30282" last="30295">
            <title>Synergic and opposing activities of thermophilic RecQ-like helicase and topoisomerase 3 proteins in Holliday junction processing and replication fork stabilization.</title>
            <authorList>
                <person name="Valenti A."/>
                <person name="De Felice M."/>
                <person name="Perugino G."/>
                <person name="Bizard A."/>
                <person name="Nadal M."/>
                <person name="Rossi M."/>
                <person name="Ciaramella M."/>
            </authorList>
            <dbReference type="PubMed" id="22722926"/>
            <dbReference type="DOI" id="10.1074/jbc.m112.366377"/>
        </citation>
        <scope>FUNCTION AS A HELICASE</scope>
        <scope>FUNCTION AS AN ATPASE</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH TOP3</scope>
        <scope>DNA-BINDING</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2018" name="Extremophiles" volume="22" first="581" last="589">
            <title>The Sulfolobus solfataricus RecQ-like DNA helicase Hel112 inhibits the NurA/HerA complex exonuclease activity.</title>
            <authorList>
                <person name="De Falco M."/>
                <person name="Massa F."/>
                <person name="Rossi M."/>
                <person name="De Felice M."/>
            </authorList>
            <dbReference type="PubMed" id="29488113"/>
            <dbReference type="DOI" id="10.1007/s00792-018-1018-7"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">DNA helicase that translocates in a 3'-5' direction on single-stranded (ss)DNA, probably involved in DNA repair. Unwinds DNA in a 3'-5' direction, unwinding is ATP-dependent, acts preferentially on fork and 3'-tailed DNA; bubble and blunt-ended double-stranded (ds)DNA are not substrates (PubMed:17683280). Has winding and unwinding activity, unwinds Holliday junction (HJ) DNA in the presence of ATP, the main product is forked DNA, single-stranded binding protein (SSB) does not stimulate activity (PubMed:22722926). Anneals complementary oligonucleotides in an ATP-independent manner to form HJ and fork structures, thus can perform strand exchange (PubMed:17683280, PubMed:22722926). Preferentially binds HJ, forked and ssDNA, dsDNA is bound less well (PubMed:17683280, PubMed:22722926). LhrC-Core (Hel112) inhibits the exonuclease activity of the HerA-NurA complex on ss- and dsDNA, has no effect on ssDNA nicking by NurA (PubMed:29488113); HerA-NurA are involved in DNA end-resection during DNA double-strand break repair (PubMed:29488113).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 8">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="5">DNA topoisomerase 3 (topA) inhibits helicase activity on Holliday junctions (HJ) but has no effect on ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5 8">Monomer and homodimer (PubMed:17683280). The monomeric form has helicase, ATPase and strand annealing activities (PubMed:17683280, PubMed:22722926), while the dimeric form only has ATPAse and strand annealing activities (PubMed:17683280). Interacts with DNA topoisomerase 3 (topA).</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Composed of 2 helicase domains, a winged-helix (WH) domain, and Lhr-specific domain 4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the Lhr helicase family. Lhr-Core subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="4"/>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75377">
        <property type="entry name" value="S75377"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988933.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95949"/>
    <dbReference type="SMR" id="P95949"/>
    <dbReference type="FunCoup" id="P95949">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0112"/>
    <dbReference type="PaxDb" id="273057-SSO0112"/>
    <dbReference type="EnsemblBacteria" id="AAK40467">
        <property type="protein sequence ID" value="AAK40467"/>
        <property type="gene ID" value="SSO0112"/>
    </dbReference>
    <dbReference type="GeneID" id="1453295"/>
    <dbReference type="KEGG" id="sso:SSO0112"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.107"/>
    <dbReference type="eggNOG" id="arCOG00557">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002025_0_0_2"/>
    <dbReference type="InParanoid" id="P95949"/>
    <dbReference type="PhylomeDB" id="P95949"/>
    <dbReference type="BRENDA" id="3.6.4.12">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990814">
        <property type="term" value="F:DNA/DNA annealing activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17922">
        <property type="entry name" value="DEXHc_LHR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18796">
        <property type="entry name" value="SF2_C_LHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052511">
        <property type="entry name" value="ATP-dep_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017170">
        <property type="entry name" value="Lhr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013701">
        <property type="entry name" value="Lhr-like_DEAD/DEAH_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045628">
        <property type="entry name" value="Lhr_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010338">
        <property type="entry name" value="PRK13767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962:SF6">
        <property type="entry name" value="LARGE HELICASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08494">
        <property type="entry name" value="DEAD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19306">
        <property type="entry name" value="WHD_Lhr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037307">
        <property type="entry name" value="Lhr-like_helic_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000102198" description="ATP-dependent helicase Lhr-Core">
        <location>
            <begin position="1"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="39"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="3">
        <location>
            <begin position="247"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="WH domain" evidence="1">
        <location>
            <begin position="420"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Domain 4" evidence="1">
        <location>
            <begin position="507"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEIH box">
        <location>
            <begin position="173"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Wedges between bases of the loading strand" evidence="1">
        <location>
            <position position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0QT91"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="17683280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="22722926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="6">
        <source>
            <dbReference type="PubMed" id="17683280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <evidence type="ECO:0000305" key="8">
        <source>
            <dbReference type="PubMed" id="22722926"/>
        </source>
    </evidence>
    <sequence length="875" mass="100393" checksum="920C7CF77BF8EC98" modified="1997-05-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="158" xmlns="https://uniprot.org/uniprot">
    <accession>Q59968</accession>
    <name>CARA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carbamoyl phosphate synthase small chain</fullName>
            <ecNumber evidence="1">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Carbamoyl phosphate synthetase glutamine chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">carA</name>
        <name type="ordered locus">SSO0640</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Biol. Evol." volume="13" first="970" last="977">
            <title>Phylogenetic analysis of carbamoylphosphate synthetase genes: complex evolutionary history includes an internal duplication within a gene which can root the tree of life.</title>
            <authorList>
                <person name="Lawson F.S."/>
                <person name="Charlebois R.L."/>
                <person name="Dillon J.A."/>
            </authorList>
            <dbReference type="PubMed" id="8752005"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <molecule>Carbamoyl phosphate synthase small chain</molecule>
        <reaction evidence="1">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="1"/>
    <dbReference type="EMBL" id="U33768">
        <property type="protein sequence ID" value="AAA99058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="T43252">
        <property type="entry name" value="T43252"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991178.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59968"/>
    <dbReference type="SMR" id="Q59968"/>
    <dbReference type="FunCoup" id="Q59968">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0640"/>
    <dbReference type="MEROPS" id="C26.A33"/>
    <dbReference type="PaxDb" id="273057-SSO0640"/>
    <dbReference type="EnsemblBacteria" id="AAK40948">
        <property type="protein sequence ID" value="AAK40948"/>
        <property type="gene ID" value="SSO0640"/>
    </dbReference>
    <dbReference type="GeneID" id="1454915"/>
    <dbReference type="GeneID" id="44129640"/>
    <dbReference type="KEGG" id="sso:SSO0640"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.645"/>
    <dbReference type="eggNOG" id="arCOG00064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035901_1_1_2"/>
    <dbReference type="InParanoid" id="Q59968"/>
    <dbReference type="PhylomeDB" id="Q59968"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005951">
        <property type="term" value="C:carbamoyl-phosphate synthase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000086">
        <property type="entry name" value="Carbamoyl-phosphate synthase small chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase small chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF7">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000112365" description="Carbamoyl phosphate synthase small chain">
        <location>
            <begin position="1"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="182"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="CPSase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01209"/>
        </source>
    </evidence>
    <sequence length="367" mass="41480" checksum="814FF3E5D2526C80" modified="1996-11-01" version="1">MKLENKKGYLYLEDGTFIEGYSFGAKGIKVGEVVFTTSMNGYVESLTDPSYKGQILIITHPLVGNYGVPEKKYEQGILTNFESERIQVEGLIVAEHTYPSKWNSALTLDEWLKSENVPGVFDVDTRMIVKKIRTYGTMMGIIASELEIDDPRKYLEKKYDEIDFTQFTSPKSPIFHPNTGDMIVVVDCGIKHGILYGLYKRGFSIVRVPCSFSASKIIEYNPKGIVFSNGPGNPNLLENQIKTFSELVEYKIPILGICLGHQIATLALGGKIKKMKFGHRAINKPVIESNSNKCYISTHNHGYGIISKNDIPPNTKIWFYNPDDYTIEGLIHEKLPIITTQFHPEARPGPWDTTWVFDKFRTMVTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX49</accession>
    <name>Q7LX49_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Endonuclease IV related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2156</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP endonuclease 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74063">
        <property type="entry name" value="S74063"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX49"/>
    <dbReference type="FunCoup" id="Q7LX49">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2156"/>
    <dbReference type="PaxDb" id="273057-SSO2156"/>
    <dbReference type="EnsemblBacteria" id="AAK42332">
        <property type="protein sequence ID" value="AAK42332"/>
        <property type="gene ID" value="SSO2156"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2156"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2249"/>
    <dbReference type="eggNOG" id="arCOG01894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068832_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX49"/>
    <dbReference type="PhylomeDB" id="Q7LX49"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.150:FF:000017">
        <property type="entry name" value="Endonuclease IV related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018246">
        <property type="entry name" value="AP_endonuc_F2_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445:SF0">
        <property type="entry name" value="APURINIC-APYRIMIDINIC ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="ENDONUCLEASE IV ENDODEOXYRIBONUCLEASE IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00729">
        <property type="entry name" value="AP_NUCLEASE_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="8">
        <location>
            <begin position="32"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="283" mass="32015" checksum="DBFEB9C13F0B5975" modified="2011-05-31" version="1">MYNKFKLMAKIYLGPAGVPHSAKKKNTVEGIRTVKELGLNAMEVEFVQGVRMSKETAEETGQVARELGVRLSVHAPYFINLCSEEKEKIEASKQRILDTADRAELMGADAIAIHIAFYGKMTPEECYQNVKEGLEEVIDKAREMGIRNVKFGVETMAKETAFGTLDEVISISKELKGVIPYIDWAHTFARQGGEIDYGKIIDRLIKELGLTHINSHFESLVYRRGKYVDEHIPIDANAPPFEPLAKELLKRDISITLICESPELERDALKMKEVLERLGYRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX52</accession>
    <name>Q7LX52_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73095">
        <property type="entry name" value="S73095"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX52"/>
    <dbReference type="PaxDb" id="273057-SSO2152"/>
    <dbReference type="EnsemblBacteria" id="AAK42328">
        <property type="protein sequence ID" value="AAK42328"/>
        <property type="gene ID" value="SSO2152"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2152"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2245"/>
    <dbReference type="eggNOG" id="arCOG08535">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2021608_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX52"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="137" mass="14983" checksum="D7E543474A3DFB95" modified="2011-05-31" version="1">MLNIYMRKGLSDSVTMMIVLLASVILAITVVSILFTYLGYFGSNYGYVKQIGTALITQNGELNITLENSFSNAKIVGVIYNATLHKVTYTISVGLNKYTINTGFTFPNGIQTVTLTLVVVTNQDTIYIPVEAQLVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX68</accession>
    <name>Q7LX68_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="3">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">porB-like</name>
        <name evidence="7" type="ordered locus">SSO2130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74042">
        <property type="entry name" value="S74042"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988324.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX68"/>
    <dbReference type="FunCoup" id="Q7LX68">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2130"/>
    <dbReference type="PaxDb" id="273057-SSO2130"/>
    <dbReference type="EnsemblBacteria" id="AAK42309">
        <property type="protein sequence ID" value="AAK42309"/>
        <property type="gene ID" value="SSO2130"/>
    </dbReference>
    <dbReference type="GeneID" id="1453636"/>
    <dbReference type="KEGG" id="sso:SSO2130"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2222"/>
    <dbReference type="eggNOG" id="arCOG01601">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058423_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX68"/>
    <dbReference type="PhylomeDB" id="Q7LX68"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051479">
        <property type="entry name" value="PorB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42897">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42897:SF2">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="6">
        <location>
            <begin position="60"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="338" mass="37972" checksum="2804427D5454FC0D" modified="2011-05-31" version="1">MVEKELEEKVYKSIIKTIKDTPLEEFYTSGHRTCQGCESALVMRFLAKAAGPRTIVIGATGCMYVANTTYYTTSWIVPWVHTQLGGTGAAALGTAAALRALMRKGKIKQEPINVIAFCGDLGCADMGLSGVSNAMTYDYNLLIILYDNESSANTDIQETSMTPFGAQTSFSRPGKQRRIMKKRWKKSVVPMIIAGHRNVRYAATMTPAYPLDSMNKIRKALTIGGPTFIHSLDPCPKGWDYDPKFSHELGVLAVETGLWPLYEYIDGEIVYNEPTKSIVEGRMKRKPVKEYLEKQGRFSHFTEEDIEYVQRMVDEMYEEWEIPGVMPIKSINAKISDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX71</accession>
    <name>Q7LX71_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transport protein, permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74039">
        <property type="entry name" value="S74039"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923780.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX71"/>
    <dbReference type="FunCoup" id="Q7LX71">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2127"/>
    <dbReference type="PaxDb" id="273057-SSO2127"/>
    <dbReference type="EnsemblBacteria" id="AAK42306">
        <property type="protein sequence ID" value="AAK42306"/>
        <property type="gene ID" value="SSO2127"/>
    </dbReference>
    <dbReference type="GeneID" id="1453633"/>
    <dbReference type="KEGG" id="sso:SSO2127"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2219"/>
    <dbReference type="eggNOG" id="arCOG00147">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_59_7_2"/>
    <dbReference type="InParanoid" id="Q7LX71"/>
    <dbReference type="PhylomeDB" id="Q7LX71"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17353">
        <property type="entry name" value="MFS_OFA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004741">
        <property type="entry name" value="Oxa_For_antiport_fam_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00890">
        <property type="entry name" value="2A0111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF304">
        <property type="entry name" value="MFS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="388"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="12"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="430" mass="46853" checksum="A7379073FDA8410E" modified="2011-05-31" version="1">MMSSKWFEKGWFRWMLVVGATISMALVSVYEYSWTLYTVPLGRVFHVAPGSPALGLTYTIYIIVQALSMFVAGRIADRYGPRLISMLGGIITGIGYISSAFAKSLPILYLTYGFGSIGVGIIYGTAISTAVKWFPDKRGLATGIIEIGFGGGSFALSPLIQYIITSISYTAAFTYMGIVQLIVITLLAFFFAYPPPQWLPKGFNAEEYEKKRKMIKRSKNDYTVSQMVKTWQWWVIYIAFFLIAGSGLSIIGHLIPYGRSLGFSIAAVIAVFLFPFANGLGRFVMGTVSDYLGRPYTMTLSFGISGISMLSIAFIPKIASLYLALIFLTAFTWGPLFSLFPPLVGDYYGPKHSGANYGLTYTAKALAGIFAGYGASILFTSYGIKETLIITGSMAIMSAILALTLRPPKIPTAQIAGAESQKGSIESKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX73</accession>
    <name>Q7LX73_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74037">
        <property type="entry name" value="S74037"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991907.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX73"/>
    <dbReference type="PaxDb" id="273057-SSO2125"/>
    <dbReference type="EnsemblBacteria" id="AAK42304">
        <property type="protein sequence ID" value="AAK42304"/>
        <property type="gene ID" value="SSO2125"/>
    </dbReference>
    <dbReference type="GeneID" id="1453631"/>
    <dbReference type="KEGG" id="sso:SSO2125"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2216"/>
    <dbReference type="eggNOG" id="arCOG05545">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1599078_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX73"/>
    <dbReference type="PhylomeDB" id="Q7LX73"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021799">
        <property type="entry name" value="PIN-like_prokaryotic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11848">
        <property type="entry name" value="DUF3368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19876" checksum="743B705EFAC10FA6" modified="2011-05-31" version="1">MIAVADTSFLIDWVRYSKRDLIFQLFNLIYLPDSVFNEVRSERTLLWIAQGLENNKLAIFPELPQIREEALRLVYETRRLPVRPVDYPEAYCVVVGKVEGYTVLTENGGAVALINYYKEYRDVKIMRALEVLIQLHNRGLITDIRNEIEEYNKQTGHRFSSKDLSGYDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXI7</accession>
    <name>Q7LXI7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid specific permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO0786</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90228">
        <property type="entry name" value="E90228"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923079.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXI7"/>
    <dbReference type="FunCoup" id="Q7LXI7">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0786"/>
    <dbReference type="PaxDb" id="273057-SSO0786"/>
    <dbReference type="EnsemblBacteria" id="AAK41084">
        <property type="protein sequence ID" value="AAK41084"/>
        <property type="gene ID" value="SSO0786"/>
    </dbReference>
    <dbReference type="GeneID" id="1455051"/>
    <dbReference type="KEGG" id="sso:SSO0786"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.789"/>
    <dbReference type="eggNOG" id="arCOG03651">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_20_3_2"/>
    <dbReference type="InParanoid" id="Q7LXI7"/>
    <dbReference type="PhylomeDB" id="Q7LXI7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="466" mass="50233" checksum="FFFABDAD68B24607" modified="2011-05-31" version="1">MDEKRELKREVLGTWLVASYGIAANAPIAVATLYFVGIAGLAGGSMPLVTVLSYLIYATTLLVVYEWSKDIAASYGYLAMIKKGLNSSLAAFTVGYGYIYQYLVAGAAGFGVLGVTSFLYLVSPSIASSMPWLWAVISVIVTLETTLIMWLGVKPGGMLNLIVGLISIVYLIITSIALIIIAGDKNTLAPFTAQPVNGNWVLILTSMIFGITTFGGATTPVGVAEEAKVPKKTLPKALLLEFLLLGVGLILNAYAQTVVYGVNNMFNYANLPDPMVIIYSKYFSTITVLILIVLVAFMFNSSTIAFATSGSRMIYGMARDGVLYPKIFTKINKYGVPGNAILLTGIITGALSLISGYILGPLEASIFLITFGSFYVSLGHLFAAIALIRHKVKVRMINIVKHVLVPIVSSIAYILTIYFGTYPAPAFPLNIAVYAAWAVLLIHVILYYILKSRNPEAIKKLGDFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXK8</accession>
    <name>Q7LXK8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA/rRNA methyltransferase SpoU type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0747</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90223">
        <property type="entry name" value="C90223"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991324.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXK8"/>
    <dbReference type="STRING" id="273057.SSO0747"/>
    <dbReference type="PaxDb" id="273057-SSO0747"/>
    <dbReference type="EnsemblBacteria" id="AAK41042">
        <property type="protein sequence ID" value="AAK41042"/>
        <property type="gene ID" value="SSO0747"/>
    </dbReference>
    <dbReference type="GeneID" id="1455009"/>
    <dbReference type="KEGG" id="sso:SSO0747"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.742"/>
    <dbReference type="eggNOG" id="arCOG01018">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056931_3_0_2"/>
    <dbReference type="InParanoid" id="Q7LXK8"/>
    <dbReference type="PhylomeDB" id="Q7LXK8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18093">
        <property type="entry name" value="SpoU-like_TrmJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004384">
        <property type="entry name" value="RNA_MeTrfase_TrmJ/LasT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053648">
        <property type="entry name" value="tRNA_Cytidine-2'-O-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00050">
        <property type="entry name" value="rRNA_methyl_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041077">
        <property type="entry name" value="tRNAmeth_TrmJ_Sulfolob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786:SF2">
        <property type="entry name" value="TRNA (CYTIDINE_URIDINE-2'-O-)-METHYLTRANSFERASE TRMJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786">
        <property type="entry name" value="TRNA/RRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004808">
        <property type="entry name" value="LasT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="5">
        <location>
            <begin position="2"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="237" mass="27290" checksum="E8E89534EF5514A8" modified="2011-05-31" version="1">MIRVVLVEPEGEYNIGFVARLCKNFLVDELYIVNPRANISEAFRFSAGGREILEKAKIVNTFDKAINDVDIKIATSSIADNNGDILRKSIRPWEILPLIQGKKIALIFGRESVGLTREEIFKCDFLLYIPANPSYPVLNLSHAVGIVLYEIWKNRELLTNPQTSTISDQSIKLIDKYTEILYKLLKRSDDDYKMYIALKRAIIKGVKDEEEARVIVHFLRKLYTRIIHNEKEMNNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXP0</accession>
    <name>Q7LXP0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Iron-dependent repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0669</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtxR/MntR family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90214">
        <property type="entry name" value="E90214"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991214.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXP0"/>
    <dbReference type="STRING" id="273057.SSO0669"/>
    <dbReference type="PaxDb" id="273057-SSO0669"/>
    <dbReference type="EnsemblBacteria" id="AAK40972">
        <property type="protein sequence ID" value="AAK40972"/>
        <property type="gene ID" value="SSO0669"/>
    </dbReference>
    <dbReference type="GeneID" id="1454939"/>
    <dbReference type="KEGG" id="sso:SSO0669"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.669"/>
    <dbReference type="eggNOG" id="arCOG02099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069532_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXP0"/>
    <dbReference type="PhylomeDB" id="Q7LXP0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.10">
        <property type="entry name" value="Iron dependent repressor, metal binding and dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050536">
        <property type="entry name" value="DtxR_MntR_Metal-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238">
        <property type="entry name" value="IRON (METAL) DEPENDENT REPRESSOR, DTXR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238:SF7">
        <property type="entry name" value="IRON-DEPENDENT TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="Iron-dependent repressor protein, dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="212" mass="24109" checksum="3090DF4F53DF3603" modified="2011-05-31" version="1">MVELSEPLENYLKEMYEIEEIKGSAKVSELISIFNISPGTISKALNKLEKLGLVERTNDRKIKLTEEGKRIAERLIKSHRLSERLLTDIVGVDWIRAHELAHRLEHIWPDDILERIDRLLGYPTTCPHGHPIGNRIKVSGKKLSEINIAGKYKVVMIVREEEWILREATRLGLKPGVVLEVIENNGTDIKIKIGEEIEEVSKVIGDQVLVIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2014-05-14" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXP6</accession>
    <name>TLDD_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Zinc metalloprotease TldD homolog</fullName>
            <ecNumber>3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">tldD</name>
        <name type="ordered locus">SSO0660</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="Biosci. Rep." volume="32" first="609" last="618">
            <title>An archaeal protein evolutionarily conserved in prokaryotes is a zinc-dependent metalloprotease.</title>
            <authorList>
                <person name="Hu Y."/>
                <person name="Peng N."/>
                <person name="Han W."/>
                <person name="Mei Y."/>
                <person name="Chen Z."/>
                <person name="Feng X."/>
                <person name="Liang Y.X."/>
                <person name="She Q."/>
            </authorList>
            <dbReference type="PubMed" id="22950735"/>
            <dbReference type="DOI" id="10.1042/BSR20120074"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>COFACTOR</scope>
        <scope>MUTAGENESIS OF HIS-228; GLU-229; HIS-233 AND CYS-416</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Zinc metalloprotease. Able to degrade azocasein in vitro.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="1">Optimum pH is 7.0.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="1">Optimum temperature is 55-65 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text>Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The HEAIGH motif probably functions as the active center of the metalloprotease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90213">
        <property type="entry name" value="F90213"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991202.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXP6"/>
    <dbReference type="SMR" id="Q7LXP6"/>
    <dbReference type="STRING" id="273057.SSO0660"/>
    <dbReference type="PaxDb" id="273057-SSO0660"/>
    <dbReference type="EnsemblBacteria" id="AAK40965">
        <property type="protein sequence ID" value="AAK40965"/>
        <property type="gene ID" value="SSO0660"/>
    </dbReference>
    <dbReference type="GeneID" id="1454932"/>
    <dbReference type="GeneID" id="44129657"/>
    <dbReference type="KEGG" id="sso:SSO0660"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.662"/>
    <dbReference type="eggNOG" id="arCOG00321">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_1_2_2"/>
    <dbReference type="InParanoid" id="Q7LXP6"/>
    <dbReference type="PhylomeDB" id="Q7LXP6"/>
    <dbReference type="BRENDA" id="3.4.24.B29">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="entry name" value="PmbA/TldD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051463">
        <property type="entry name" value="Peptidase_U62_metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053642">
        <property type="entry name" value="Zinc_metalloprotease_TldD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040952">
        <property type="entry name" value="Arch_mtprotase_TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624:SF0">
        <property type="entry name" value="METALLOPROTEASE SLR0863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN TLDD AND PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000429058" description="Zinc metalloprotease TldD homolog">
        <location>
            <begin position="1"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HEAIGH motif">
        <location>
            <begin position="228"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abolishes metalloprotease activity. Does not affect homodimerization." evidence="1">
        <original>H</original>
        <variation>F</variation>
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abolishes metalloprotease activity. Does not affect homodimerization." evidence="1">
        <original>E</original>
        <variation>D</variation>
        <location>
            <position position="229"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abolishes metalloprotease activity. Does not affect homodimerization." evidence="1">
        <original>H</original>
        <variation>Y</variation>
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abolishes metalloprotease activity and homodimerization." evidence="1">
        <original>C</original>
        <variation>G</variation>
        <location>
            <position position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="22950735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="443" mass="49882" checksum="34D565FE315CAF03" modified="2011-05-31" version="1">MFKYIKKAEELGASFADIRYERVIANEVTITEDRKYVSHGVDEGYSIRVIYNRNWGFKATDKITENEIEDTINHIYGDERVNIVYLPSKHDTIKIGKDINKNEEEKINDLHKVASQVNTLHPSIKSYSIKYYDEIFHKEYYSSEDREIIADGSSSSLSILVVAREGDVTVEVSEILSTQMGYIFDVFDINQVLANLQKRIINQLKGSTPKAGEYPVILAPEVVGIFTHEAIGHLSEADVTLNGSLYKLRNKRIGDEFLNISDLPAMDHPQSSLVYYDDEGVEGREVKIIENGILKEFMTDRYYSAYLGQPPTGNARAQSYRNFSLIRMRNTYMKPGDASLNELFEGIKEGYYLVSPIGGETSSNGTFQFAIQEGYRVENSEIKEPLRNIGISGNTISTLNAIEMISKDFGMSSGFCEKNGQTVQVSNGGPHIKVKKLKVGGYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXQ8</accession>
    <name>Q7LXQ8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-gulonate 3-dehydrogenase</fullName>
            <ecNumber evidence="8">1.1.1.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">L-gulonate 3-dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">hdb-1</name>
        <name evidence="13" type="ordered locus">SSO0647</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.45" evidence="8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99212">
        <property type="entry name" value="A99212"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991183.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXQ8"/>
    <dbReference type="FunCoup" id="Q7LXQ8">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0647"/>
    <dbReference type="PaxDb" id="273057-SSO0647"/>
    <dbReference type="EnsemblBacteria" id="AAK40952">
        <property type="protein sequence ID" value="AAK40952"/>
        <property type="gene ID" value="SSO0647"/>
    </dbReference>
    <dbReference type="GeneID" id="1454919"/>
    <dbReference type="KEGG" id="sso:SSO0647"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.650"/>
    <dbReference type="eggNOG" id="arCOG00251">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_0_1_2"/>
    <dbReference type="InParanoid" id="Q7LXQ8"/>
    <dbReference type="PhylomeDB" id="Q7LXQ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050104">
        <property type="term" value="F:L-gulonate 3-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022694">
        <property type="entry name" value="3-OHacyl-CoA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006180">
        <property type="entry name" value="3-OHacyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075:SF1">
        <property type="entry name" value="LAMBDA-CRYSTALLIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000105">
        <property type="entry name" value="HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00067">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="12">
        <location>
            <begin position="10"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="193"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="10">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAK40952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="324" mass="36626" checksum="57400DA44A8594A8" modified="2011-05-31" version="1">MFIHMKSINKVAVIGAGVIGVGWTTLLLAKGYKVNLYTEKKETLEKALAKVSAYLVNLKNLGMINEEPESYITNLTGITKIDDAIHNVDFVIEAIIEDYTAKKNLFKLLDTQLPQDIIIASSTSGLLMTEIQKAMIRHPERGVIAHPWNPPHLLPLVEIVPGEKTSKETVDLTREFMEKLDRVVVLLRKEVPGFIGNRLAFALFREAVNLVDEGVATVEDIDKVMTAAIGLRWAFMGPFLTYHLGGGEGGIEYFFSKGFGYGANEWMHTLAKWDKFPYTAVKKVLEQMKEYEFIKGKSFQDLSRWRDEKLVTIYKAVYKEKCNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-01-24" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXW6</accession>
    <name>Q7LXW6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in a partial transposon ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90198">
        <property type="entry name" value="E90198"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXW6"/>
    <dbReference type="PaxDb" id="273057-SSO0525"/>
    <dbReference type="EnsemblBacteria" id="AAK40844">
        <property type="protein sequence ID" value="AAK40844"/>
        <property type="gene ID" value="SSO0525"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0525"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.523"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140802_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXW6"/>
    <dbReference type="PhylomeDB" id="Q7LXW6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="158" mass="18732" checksum="B4978272254DEF45" modified="2011-05-31" version="1">MQGFSYKCYWKRKGIKMRSLDFVYPLRLPLLVEIADLRSDSPSQFLLRSVREVSQYMEIDYVVADAGFLNLDAIKEMPVKTIVRGKSNLKRSKELSNVPLVEKRYEVKDKVYVAYRVLKFEGLYYYDVVYVKGKPRHFIFVTNFEGDPYELAELYRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXX8</accession>
    <name>Q7LXX8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75371">
        <property type="entry name" value="S75371"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXX8"/>
    <dbReference type="STRING" id="273057.SSO0118"/>
    <dbReference type="PaxDb" id="273057-SSO0118"/>
    <dbReference type="EnsemblBacteria" id="AAK40473">
        <property type="protein sequence ID" value="AAK40473"/>
        <property type="gene ID" value="SSO0118"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0118"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.113"/>
    <dbReference type="eggNOG" id="arCOG05987">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987669_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXX8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF046073">
        <property type="entry name" value="UpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="154" mass="16590" checksum="C57BCB2B792F37E7" modified="2011-05-31" version="1">MLQLMMKGGYKLKKRKGLSSILGTVIVLAITLVLGGLLYAYSNGLFSSLTQNASLQTQVSIYVNPNTGQAYIQYYISNTGSTQIYLSSIQILNGTRNIIIPLNNNLLQPGQTVQNITSINGKITAGQYYTVEIAGNLPNGKPYSVVQNVLASIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXY4</accession>
    <name>Q7LXY4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0110</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75379">
        <property type="entry name" value="S75379"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988929.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXY4"/>
    <dbReference type="FunCoup" id="Q7LXY4">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0110"/>
    <dbReference type="PaxDb" id="273057-SSO0110"/>
    <dbReference type="DNASU" id="1453293"/>
    <dbReference type="EnsemblBacteria" id="AAK40465">
        <property type="protein sequence ID" value="AAK40465"/>
        <property type="gene ID" value="SSO0110"/>
    </dbReference>
    <dbReference type="GeneID" id="1453293"/>
    <dbReference type="KEGG" id="sso:SSO0110"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.106"/>
    <dbReference type="eggNOG" id="arCOG00610">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_926289_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXY4"/>
    <dbReference type="PhylomeDB" id="Q7LXY4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04588">
        <property type="entry name" value="CBS_pair_archHTH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051462">
        <property type="entry name" value="CBS_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016436">
        <property type="entry name" value="UCP005063_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005104">
        <property type="entry name" value="WHTH_HrcA_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108:SF23">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03444">
        <property type="entry name" value="WHD_HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005063">
        <property type="entry name" value="UCP005063_CBS_MJ1232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="178"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="241"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="300" mass="33521" checksum="DFE53515F5DF9BF4" modified="2011-05-31" version="1">MQNLSPTQREILLALTDLYNRQKRMIKSKEVADIIGKDEGTVRNIILSLKVLGLVESKPGPNGGYMPTLKAYEIINNPTLTPILDKLNLYKGMIETDIKVENIEIIDITNPSANRVLLKVEGDLRKLKIGDAVRLGPTPYSRLVIEGLILHLDENSKEIVVDVKRMISIPKEKVKNLISKKLIALKPETSLREASMIFYKEAIRGAPVINQDEKVVGILTTADIIKAFFEGNYTAKVSDYMKTNVISINENEDLLDAIRKMIIYNVGRLLVLDSNNKAVGIVTRTDILRSIAGLEGLWTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXY9</accession>
    <name>Q7LXY9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Esterase, tropinesterase related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75387">
        <property type="entry name" value="S75387"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXY9"/>
    <dbReference type="FunCoup" id="Q7LXY9">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0102"/>
    <dbReference type="PaxDb" id="273057-SSO0102"/>
    <dbReference type="EnsemblBacteria" id="AAK40458">
        <property type="protein sequence ID" value="AAK40458"/>
        <property type="gene ID" value="SSO0102"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0102"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.99"/>
    <dbReference type="eggNOG" id="arCOG01648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_4_2"/>
    <dbReference type="InParanoid" id="Q7LXY9"/>
    <dbReference type="PhylomeDB" id="Q7LXY9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF31">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN YCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="2"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="231" mass="26293" checksum="7E60691B48401A7C" modified="2011-05-31" version="1">MLIHHLAGSYKSWKFVIPKLSLDNTVVAYDLRGHGRSSTPNSPYNIEDHSNDLRRLLVQLGIEKPVLIGHSIGSLIAIDYALKYPIEKLVLIGALYRAPSSEVYEKYVRIAVNFGMRALAEYRRFHKEFTETLASNYQAWNSLLEVYEETTPIGYKNAVEGLLKAKDYSDELMGINAKTLIVYGTYDGLIVNLNVFKNNMKNVEIKTIDGYGHFLNFENPLLLSEIIKNFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-03-27" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXZ9</accession>
    <name>Q7LXZ9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75411">
        <property type="entry name" value="S75411"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXZ9"/>
    <dbReference type="STRING" id="273057.SSO0077"/>
    <dbReference type="PaxDb" id="273057-SSO0077"/>
    <dbReference type="EnsemblBacteria" id="AAK40438">
        <property type="protein sequence ID" value="AAK40438"/>
        <property type="gene ID" value="SSO0077"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0077"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.77"/>
    <dbReference type="eggNOG" id="arCOG05889">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168027_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXZ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="137" mass="16252" checksum="0389A414EDB5630C" modified="2011-05-31" version="1">MFIMKLRDNNSFFSSSGVINMGQKLPPTNKPSFEKLYDCETESCIQKLVDDYIYNLITDNNNINNYTTFKLFKDEDDNEHKFVLNEKVMLNSYLFIKVLLRRILETDLDFEYVVEDLEKKLGNTHPVVLFLKQFKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-03-27" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LY01</accession>
    <name>Q7LY01_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75423">
        <property type="entry name" value="S75423"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922873.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LY01"/>
    <dbReference type="STRING" id="273057.SSO0065"/>
    <dbReference type="PaxDb" id="273057-SSO0065"/>
    <dbReference type="EnsemblBacteria" id="AAK40426">
        <property type="protein sequence ID" value="AAK40426"/>
        <property type="gene ID" value="SSO0065"/>
    </dbReference>
    <dbReference type="GeneID" id="1455317"/>
    <dbReference type="KEGG" id="sso:SSO0065"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.65"/>
    <dbReference type="eggNOG" id="arCOG05890">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237967_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LY01"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="240" mass="27892" checksum="EAFEE9AA97A31750" modified="2011-05-31" version="1">MQLLVILRDLKIQLWVILQRGLEVKVPFLGIPLKGVNNPILVLDGIIEPLNIFVNTEAIRQFIMQFNEAVGFECIKEEVYWDSSIPFSSYYIYITDKLANDAIRRCGIPISEDERFEILHLVDEAIFPPNFLVKALRTSLQLNSPILFRDGEEPITVQLEPIRIKIISSYPFDNNPKYLDNSLVHLAGIIPVEYIESKSRNLIEVENGLWSAIYSLPYLQINNWKWIWDLNWVTIIEFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TU9</accession>
    <name>Q97TU9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1709</name>
        <name evidence="2" type="ordered locus">SSO1790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90332">
        <property type="entry name" value="C90332"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992505.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TU9"/>
    <dbReference type="PaxDb" id="273057-SSO1709"/>
    <dbReference type="EnsemblBacteria" id="AAK41914">
        <property type="protein sequence ID" value="AAK41914"/>
        <property type="gene ID" value="SSO1709"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41984">
        <property type="protein sequence ID" value="AAK41984"/>
        <property type="gene ID" value="SSO1790"/>
    </dbReference>
    <dbReference type="GeneID" id="1454763"/>
    <dbReference type="KEGG" id="sso:SSO1709"/>
    <dbReference type="KEGG" id="sso:SSO1790"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1758"/>
    <dbReference type="eggNOG" id="arCOG11547">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369589_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="199" mass="23241" checksum="D57252EEE01CBB53" modified="2001-10-01" version="1">MKFSSNATTEIVFGDGSFDREIVRNALEKNKDKYCELLLFIPESGAKGKSLPIGIGIESVLKFVKNDVKRFSLNYIIFIDKEHCTLDYVECVKETAKDYGIYTSDVIKLNNEYDIYRFSCSVGSKKFRMYFVFLGYYCCIEDFILKICNEQIKEFRGRGCCQEYKKELKKIKDNCIEKAIDELFKEIENVLKDIRQTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TZ4</accession>
    <name>Q97TZ4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sorbitol dehydrogenase</fullName>
            <ecNumber evidence="7">1.1.1.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO3237</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.14" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90509">
        <property type="entry name" value="C90509"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989673.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TZ4"/>
    <dbReference type="SMR" id="Q97TZ4"/>
    <dbReference type="FunCoup" id="Q97TZ4">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3237"/>
    <dbReference type="PaxDb" id="273057-SSO3237"/>
    <dbReference type="EnsemblBacteria" id="AAK43330">
        <property type="protein sequence ID" value="AAK43330"/>
        <property type="gene ID" value="SSO3237"/>
    </dbReference>
    <dbReference type="GeneID" id="1453249"/>
    <dbReference type="KEGG" id="sso:SSO3237"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3337"/>
    <dbReference type="eggNOG" id="arCOG01459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_0_2"/>
    <dbReference type="InParanoid" id="Q97TZ4"/>
    <dbReference type="PhylomeDB" id="Q97TZ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030554">
        <property type="term" value="F:adenyl nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043168">
        <property type="term" value="F:anion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003939">
        <property type="term" value="F:L-iditol 2-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08235">
        <property type="entry name" value="iditol_2_DH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF2">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="6">
        <location>
            <begin position="16"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="345" mass="37555" checksum="7E75CEA343F2A54B" modified="2001-10-01" version="1">MVEGFSPMKSVIMENGKAVIKELPLPKLQQGDVLVKMKACGLCGTDIEKICGQYTASQPILGHEPTGIIQESTVDWLKPGDRVFAHHHVPCYECYYCKKGSPTMCPYYRKTNLDPGGFSEYFRVPAWNVARGGVLKLPDNVSFEEGAFIEPLATVIRAQKRVAIDNGDTVFIVGIGPMGLLHAMMAKVNKAGVIIASDVSDFRTEFGYKVGINYSLNAKKVNVVDEVKRLTDGRGADVTIIASGSPSAIVSGLNATRKGGRVLLFGVPYKGTILNYDISDLLNNEISIIPSNAAVEEDTKEALAIIANKKIDVTKLITHRFSLDEFNEAVRIGKEGNAIKVIIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U04</accession>
    <name>Q97U04_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycosyltransferase RgtA/B/C/D-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO3225</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90508">
        <property type="entry name" value="A90508"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989685.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U04"/>
    <dbReference type="STRING" id="273057.SSO3225"/>
    <dbReference type="PaxDb" id="273057-SSO3225"/>
    <dbReference type="DNASU" id="1453239"/>
    <dbReference type="EnsemblBacteria" id="AAK43320">
        <property type="protein sequence ID" value="AAK43320"/>
        <property type="gene ID" value="SSO3225"/>
    </dbReference>
    <dbReference type="GeneID" id="1453239"/>
    <dbReference type="KEGG" id="sso:SSO3225"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3327"/>
    <dbReference type="eggNOG" id="arCOG00561">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032023_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U04"/>
    <dbReference type="PhylomeDB" id="Q97U04"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050297">
        <property type="entry name" value="LipidA_mod_glycosyltrf_83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038731">
        <property type="entry name" value="RgtA/B/C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33908">
        <property type="entry name" value="MANNOSYLTRANSFERASE YKCB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33908:SF11">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13231">
        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase RgtA/B/C/D-like" evidence="9">
        <location>
            <begin position="80"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK43320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="372" mass="42695" checksum="AF6AE499FA8B9420" modified="2001-10-01" version="1">MQLKWINFFFYLSLIVLISVYTYETVITFSPINAYIGDEVWYPTAAYNMLKLVFHVTPPPMTNIGYPNEQNIQTYINPEHPPLAKYIMAVFIYFVGYYPIVWRIPSWILGDLMLIVGYFLGKKLVSNGILGNLAGILTALLIALDPNVWSLHGIALLDIYVSFFSFLSLYFLLNDKPLWASIALALAMLSKESAYVLIFPFLYYLGEISNNVKKRAIYGIGIPALIYILFSTPLIAYYGGIKAWLDATIIHRALWDVTNGHITLTATSQISTPWDWFLNIHPFYLGYNLYASTNPYVLILWIITTPLAFIFRDSKLITTTMWAWTMWIGFVIVYILGNHTLFSFYVTDFMPVVDAYVVVSLFKLVQKINILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U32</accession>
    <name>Q97U32_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Formate dehydrogenase (Amino end)</fullName>
            <ecNumber evidence="3">1.2.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO3191</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.1.2" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90504">
        <property type="entry name" value="C90504"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U32"/>
    <dbReference type="STRING" id="273057.SSO3191"/>
    <dbReference type="PaxDb" id="273057-SSO3191"/>
    <dbReference type="EnsemblBacteria" id="AAK43290">
        <property type="protein sequence ID" value="AAK43290"/>
        <property type="gene ID" value="SSO3191"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3191"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3296"/>
    <dbReference type="eggNOG" id="arCOG01491">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_747265_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U32"/>
    <dbReference type="PhylomeDB" id="Q97U32"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.228.10">
        <property type="entry name" value="Dimethylsulfoxide Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050612">
        <property type="entry name" value="Prok_Mopterin_Oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43742:SF6">
        <property type="entry name" value="OXIDOREDUCTASE YYAE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43742">
        <property type="entry name" value="TRIMETHYLAMINE-N-OXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="370" mass="42530" checksum="4936F02BE8E8BED7" modified="2001-10-01" version="1">MVHACTRDCYDTCIFDDNHKPIDTFPFNGFTCSRGIADLKRNSLNRVDSVYIEGKLSNIDEALDLIVKEIRKRKKEEILHVDYDGNQGLLTWYYPARLWNVIGAASTDYSICSSEGHEAIKLHYGNSIGALPEDFLKYESFVIWGSELVFSFIHGWNLIRDKYKATIDVRISETAKRSNKYYIVKPGSDVYLAIGIIKRLFERNWADLSLLDEPEELRNYVYSFNDEEIEEATGLDSSKIDELAELYYYRRPLTIIGFALGRSINGGDAISLISLIPALIGMKKGFFYANSQGLGIDFKYLPRTSQILSFKNCRYAEVGKEIEEGKITFMFVWNSNPLHSLPMSDRIYDAVKEVRLFLVVHDPYCQKLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U37</accession>
    <name>Q97U37_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90503">
        <property type="entry name" value="F90503"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991703.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U37"/>
    <dbReference type="STRING" id="273057.SSO3185"/>
    <dbReference type="PaxDb" id="273057-SSO3185"/>
    <dbReference type="EnsemblBacteria" id="AAK43285">
        <property type="protein sequence ID" value="AAK43285"/>
        <property type="gene ID" value="SSO3185"/>
    </dbReference>
    <dbReference type="GeneID" id="1453204"/>
    <dbReference type="KEGG" id="sso:SSO3185"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3290"/>
    <dbReference type="eggNOG" id="arCOG05926">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2230497_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U37"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="105" mass="12497" checksum="86064ABDFF66D606" modified="2001-10-01" version="1">MEDVKNVLWKVLNNEAPLVEDDIKMYHIKEGILTEDDLKKWREAIRLIREAYYDAYKNENVAVEKVRKSLEIINSISPKKPMPPEMKIRFEDLKKNLELVVKINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U47</accession>
    <name>Q97U47_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3177</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90502">
        <property type="entry name" value="D90502"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991716.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U47"/>
    <dbReference type="PaxDb" id="273057-SSO3177"/>
    <dbReference type="EnsemblBacteria" id="AAK43275">
        <property type="protein sequence ID" value="AAK43275"/>
        <property type="gene ID" value="SSO3177"/>
    </dbReference>
    <dbReference type="GeneID" id="1453194"/>
    <dbReference type="KEGG" id="sso:SSO3177"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3281"/>
    <dbReference type="eggNOG" id="arCOG06029">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116131_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U47"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="162" mass="17284" checksum="D6AC3EEBB7CF28AA" modified="2001-10-01" version="1">MKTSILALTLVGAFLAGLATAGVAGYGPLAYISYHIMVSQQKGQAQVIPAYINLGNLTAGEKGNITATAAINISTNGTYTIDLLHADKLQKIFSNFTVELKINNYTIILTLENDSAHVSLVNGSYNVSILIIYQVSQHPSGDLNVNNEPLLIIHPQGEDNNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UA9</accession>
    <name>Q97UA9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Class I SAM-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90494">
        <property type="entry name" value="E90494"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990932.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UA9"/>
    <dbReference type="STRING" id="273057.SSO3110"/>
    <dbReference type="PaxDb" id="273057-SSO3110"/>
    <dbReference type="EnsemblBacteria" id="AAK43212">
        <property type="protein sequence ID" value="AAK43212"/>
        <property type="gene ID" value="SSO3110"/>
    </dbReference>
    <dbReference type="GeneID" id="12419125"/>
    <dbReference type="KEGG" id="sso:SSO3110"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3216"/>
    <dbReference type="eggNOG" id="arCOG03850">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1393635_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UA9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13578">
        <property type="entry name" value="Methyltransf_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23386" checksum="8CCA7582B2EFCBDC" modified="2001-10-01" version="1">MENSESILNALKEIFNVDLSKYWNEAEEINNGIKRVLGNSFRYALSETKRYVLYSIIKYYNPDIVIETGVGPGVSSTIILSALERGTLHSIDVRETLENGKQVGFLVPEQLKSKWKLYIGRSRDILLDLLKKLGKVDIFLHDSEHTFENVTFELNIAWDYLRNGGILLIDNLDFTEAPYYFTKQKGVKLYELTNEAGGLGIIIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UC8</accession>
    <name>Q97UC8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90492">
        <property type="entry name" value="B90492"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UC8"/>
    <dbReference type="STRING" id="273057.SSO3087"/>
    <dbReference type="PaxDb" id="273057-SSO3087"/>
    <dbReference type="EnsemblBacteria" id="AAK43193">
        <property type="protein sequence ID" value="AAK43193"/>
        <property type="gene ID" value="SSO3087"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3087"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3195"/>
    <dbReference type="eggNOG" id="arCOG07340">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2044515_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UC8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="133" mass="15815" checksum="52BBD07A0CA44271" modified="2001-10-01" version="1">MQKFIWTKRVFPIMELKDECGCIVNRKYASDFLMKRLFSYKKKINAKKLGYFRIDNSRWFTLYRAQDGKIYYESSECPLLIITLEALCERYIENEGKINRDNSMEKLRQIKGFTTNQIVNEVVEKFINGVSNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UD2</accession>
    <name>Q97UD2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO11934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90491">
        <property type="entry name" value="F90491"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UD2"/>
    <dbReference type="STRING" id="273057.SSO11934"/>
    <dbReference type="PaxDb" id="273057-SSO11934"/>
    <dbReference type="EnsemblBacteria" id="AAK43189">
        <property type="protein sequence ID" value="AAK43189"/>
        <property type="gene ID" value="SSO11934"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11934"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3191"/>
    <dbReference type="eggNOG" id="arCOG08313">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2434006_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UD2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="95" mass="10874" checksum="CED3351F9D2E38C4" modified="2001-10-01" version="1">MAIMISLPIIRRLLAPLVVSLFALGWYGFSVQYIVSNNNVALENGVFSAYISPSQLQGYIEATRYICYVVVYLGLIFFWYNLVKTVRELEEANKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UH2</accession>
    <name>Q97UH2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter, permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO3047</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90486">
        <property type="entry name" value="D90486"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UH2"/>
    <dbReference type="FunCoup" id="Q97UH2">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3047"/>
    <dbReference type="PaxDb" id="273057-SSO3047"/>
    <dbReference type="EnsemblBacteria" id="AAK43147">
        <property type="protein sequence ID" value="AAK43147"/>
        <property type="gene ID" value="SSO3047"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3047"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3144"/>
    <dbReference type="eggNOG" id="arCOG00748">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_8_0_2"/>
    <dbReference type="InParanoid" id="Q97UH2"/>
    <dbReference type="PhylomeDB" id="Q97UH2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050366">
        <property type="entry name" value="BP-dependent_transpt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386:SF1">
        <property type="entry name" value="D,D-DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DDPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN APPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="270"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="98"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK43147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="326" mass="36704" checksum="8DF3F3FB74F8463F" modified="2001-10-01" version="1">MYKMASRKLNTFNLKIDKYGPVYNLIRGVWEQRVSRVGFYILLGIIVFSLIGVFYTPYNPSATNFPRDLPPSPQHLLGTDDYGHDIFSQLLVGGFPVIGVGFAVGLIGTLIAILIGVTAGLYAETIIDRILSAITQIFLIIPGILIIELIGAYLGALQFKLGYITILLTLSLTGWAWGARVLRSLVLSLRRREYILSSDLIGESKLSVIFNQIIPNNLPFIASNFFFTAIYGIAGFTFIYYFGLGSLTQVNWGTMLYWSLGGEAYLRGLWWWYIPPGLMIGLVAFSFALINIGIDRVANPRLRIWDVKKYMKKIEKTKEKEEVMTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UQ4</accession>
    <name>Q97UQ4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase beta nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90474">
        <property type="entry name" value="G90474"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924051.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UQ4"/>
    <dbReference type="SMR" id="Q97UQ4"/>
    <dbReference type="PaxDb" id="273057-SSO2952"/>
    <dbReference type="DNASU" id="1452973"/>
    <dbReference type="EnsemblBacteria" id="AAK43054">
        <property type="protein sequence ID" value="AAK43054"/>
        <property type="gene ID" value="SSO2952"/>
    </dbReference>
    <dbReference type="GeneID" id="1452973"/>
    <dbReference type="KEGG" id="sso:SSO2952"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3039"/>
    <dbReference type="eggNOG" id="arCOG02110">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1860803_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UQ4"/>
    <dbReference type="PhylomeDB" id="Q97UQ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041633">
        <property type="entry name" value="Polbeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052930">
        <property type="entry name" value="TA_antitoxin_MntA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43852">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43852:SF3">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18765">
        <property type="entry name" value="Polbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase beta nucleotidyltransferase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="134" mass="15871" checksum="BE23398F2B3A4F7D" modified="2001-10-01" version="1">MLKLSDYYEKLRKFNWASYDVYYAILFGSLAKKGEGNDLDIAVEFKRKSLEAYSKLYSDLVDYLGIEEVDLAIITDETDCFFIHEVFNNSLILHLEDYIRMHRRVIICEDFLIDVKKLNTIENSARALLRKWNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UT4</accession>
    <name>Q97UT4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sulfite reductase hemoprotein beta component (CysI)</fullName>
            <ecNumber evidence="7">1.8.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cysI</name>
        <name evidence="7" type="ordered locus">SSO2909</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.1.2" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90470">
        <property type="entry name" value="C90470"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993314.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UT4"/>
    <dbReference type="FunCoup" id="Q97UT4">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2909"/>
    <dbReference type="PaxDb" id="273057-SSO2909"/>
    <dbReference type="DNASU" id="1452937"/>
    <dbReference type="EnsemblBacteria" id="AAK43018">
        <property type="protein sequence ID" value="AAK43018"/>
        <property type="gene ID" value="SSO2909"/>
    </dbReference>
    <dbReference type="GeneID" id="1452937"/>
    <dbReference type="KEGG" id="sso:SSO2909"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2999"/>
    <dbReference type="eggNOG" id="arCOG02055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001975_3_2_2"/>
    <dbReference type="InParanoid" id="Q97UT4"/>
    <dbReference type="PhylomeDB" id="Q97UT4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004783">
        <property type="term" value="F:sulfite reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="entry name" value="Sulfite Reductase Hemoprotein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.480.10">
        <property type="entry name" value="Sulfite Reductase Hemoprotein,Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045169">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493:SF54">
        <property type="entry name" value="ANAEROBIC SULFITE REDUCTASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] SUBUNIT BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="Nitrite and sulphite reductase 4Fe-4S domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="Nitrite/Sulfite reductase N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrite/Sulfite reductase ferredoxin-like" evidence="6">
        <location>
            <begin position="80"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Nitrite/sulphite reductase 4Fe-4S" evidence="5">
        <location>
            <begin position="157"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Nitrite/Sulfite reductase ferredoxin-like" evidence="6">
        <location>
            <begin position="371"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="630" mass="72474" checksum="2D45856C72D45C53" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UY0</accession>
    <name>Q97UY0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methanethiol oxidase</fullName>
            <ecNumber evidence="3">1.8.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2860</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>methanethiol + O2 + H2O = hydrogen sulfide + formaldehyde + H2O2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16007"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="EC" id="1.8.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Organosulfur degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the selenium-binding protein family.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.4" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99464">
        <property type="entry name" value="A99464"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989842.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UY0"/>
    <dbReference type="FunCoup" id="Q97UY0">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2860"/>
    <dbReference type="PaxDb" id="273057-SSO2860"/>
    <dbReference type="EnsemblBacteria" id="AAK42968">
        <property type="protein sequence ID" value="AAK42968"/>
        <property type="gene ID" value="SSO2860"/>
    </dbReference>
    <dbReference type="GeneID" id="1452887"/>
    <dbReference type="KEGG" id="sso:SSO2860"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2947"/>
    <dbReference type="eggNOG" id="arCOG04697">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032512_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UY0"/>
    <dbReference type="PhylomeDB" id="Q97UY0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018549">
        <property type="term" value="F:methanethiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008430">
        <property type="term" value="F:selenium binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008826">
        <property type="entry name" value="Se-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23300">
        <property type="entry name" value="METHANETHIOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23300:SF0">
        <property type="entry name" value="METHANETHIOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05694">
        <property type="entry name" value="SBP56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75011">
        <property type="entry name" value="3-carboxy-cis,cis-mucoante lactonizing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="466" mass="53110" checksum="3CCB7AEAA6E9240F" modified="2001-10-01" version="1">MELPSVFAPFKRDPTFYPSPKMAMKSPPEDLAYVACLYTGTGINRPDFIAVVDVKPESETYSKVIHKVELSYVNDELHHFGWNACSSALCPNGRPNFERRFLVVPGLRSSRIYIVDTKLNPRQPNIVKTIEPEEVKKVTGYSRLHTVHCGPDGIYISAFGNENGEGPGGILMLDHYSFEPLGKWEIDRSDQYLAYDFWWNLPNEVMVTSEWAVPNTIENGLRLEHLKDRYGNRIHFWDLRRRKKVSSVTLGEENRMALELRPLHDPTKLMGFINMVVSLKDLSSSIWLWYYEDGKWNGEKVIEIPAEPTEGGLPEILKPFKAVPPLVTDIDLSLDDKFLYVSLWGIGEIRQYDVSNPFKPVLTGKVKLGGIFHRADHPSDHKLTGAPQMIEISRDGKRVYVTNSLYSTWDNQFYPEGLKGWMVKLNANPDGGLDVDKEFFVDFGEARSHQVRLRGGDASSDSYCYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V14</accession>
    <name>Q97V14_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2822</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90459">
        <property type="entry name" value="F90459"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989020.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V14"/>
    <dbReference type="STRING" id="273057.SSO2822"/>
    <dbReference type="PaxDb" id="273057-SSO2822"/>
    <dbReference type="EnsemblBacteria" id="AAK42933">
        <property type="protein sequence ID" value="AAK42933"/>
        <property type="gene ID" value="SSO2822"/>
    </dbReference>
    <dbReference type="GeneID" id="1452852"/>
    <dbReference type="KEGG" id="sso:SSO2822"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2908"/>
    <dbReference type="eggNOG" id="arCOG02113">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2067846_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V14"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012440">
        <property type="entry name" value="DUF1641"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38433">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38433:SF1">
        <property type="entry name" value="DUF1641 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07849">
        <property type="entry name" value="DUF1641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13069" checksum="8391A356DFE3364D" modified="2001-10-01" version="1">MEEGGVQAIDGLIEQLIESREALENFLKLINTLQKTGILPLLVGMMNTFDQNLAFLAEQNAVLIRNINIIYSILSGKEEAKDISLTELTKQLNDPDVKRGLYLLLKILKAIGSASKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V84</accession>
    <name>Q97V84_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2751</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90450">
        <property type="entry name" value="F90450"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990260.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V84"/>
    <dbReference type="STRING" id="273057.SSO2751"/>
    <dbReference type="PaxDb" id="273057-SSO2751"/>
    <dbReference type="EnsemblBacteria" id="AAK42861">
        <property type="protein sequence ID" value="AAK42861"/>
        <property type="gene ID" value="SSO2751"/>
    </dbReference>
    <dbReference type="GeneID" id="1452780"/>
    <dbReference type="KEGG" id="sso:SSO2751"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2830"/>
    <dbReference type="eggNOG" id="arCOG03737">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1599090_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V84"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="166" mass="19572" checksum="4754C13DD6790114" modified="2001-10-01" version="1">MIGKDDVFALILSEYKDSQKPVSLSKIKRKFKDRNLIHVLEELEKEGKIRRVENGSKITFEPLDSINIEDELKILRDEIHKMLDLLQKFVESKSFSSKDFDEAYDRIRDSLGYAPLERIRIELGLSKEEFYSKFRKYVEENYDLIAGGDEGFTRRGVTYGIVKRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VB9</accession>
    <name>Q97VB9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Permease, multidrug resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO2716</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. EmrB family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90446">
        <property type="entry name" value="C90446"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988645.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VB9"/>
    <dbReference type="FunCoup" id="Q97VB9">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2716"/>
    <dbReference type="PaxDb" id="273057-SSO2716"/>
    <dbReference type="EnsemblBacteria" id="AAK42826">
        <property type="protein sequence ID" value="AAK42826"/>
        <property type="gene ID" value="SSO2716"/>
    </dbReference>
    <dbReference type="GeneID" id="1452745"/>
    <dbReference type="KEGG" id="sso:SSO2716"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2797"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="InParanoid" id="Q97VB9"/>
    <dbReference type="PhylomeDB" id="Q97VB9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17321">
        <property type="entry name" value="MFS_MMR_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000503">
        <property type="entry name" value="Drug resistance transporter, EmrB/QacA subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000021">
        <property type="entry name" value="Drug resistance transporter, EmrB/QacA subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="6"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="469" mass="50070" checksum="554D56BA7585B6A1" modified="2001-10-01" version="1">MNTNSILIVLTLGSLMAAVDTTIVLLALPTITSSLHTDLLSSIWVLLAYMIVISVLSTQAGRIGDLFGKGKIYNLGFVIFTAASALCGISSNIDMLIVFRIIQAIGGSMLVANSSSIVADVFPPNRRGKAYGITSLGWNIGALVGIVLGGVLTTFFGWQYIFYINVPIGIVAVLLGVMNIKDINKVNTKLDMLGAILLGISLALISLSLMFIAASGISTDNIIELALGVALIPFFLLNETRSKYPILNLKIFKIRLLTYSILANFLQGIGGLSLSFLLIMYLQGVRGLSPLDSSLLLTPGYVIASILAPFMGRVADRGKPGIVAGIGLMFIFITMILYFFLLTPTTDYYLIVGISAITGIGSAMFWPSNSTAIMFHAPKEYYGSVSGLSRTLGNIGTILSYVLSIVVATLSIPRSVAFEIFIGTTTLNGDVSVTFVNGLHFAFLISSIIIVVAMIFSFMSGKTQESAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VC0</accession>
    <name>Q97VC0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ABC transporter, permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">SSO2714</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. MalFG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90446">
        <property type="entry name" value="B90446"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988643.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VC0"/>
    <dbReference type="STRING" id="273057.SSO2714"/>
    <dbReference type="PaxDb" id="273057-SSO2714"/>
    <dbReference type="EnsemblBacteria" id="AAK42825">
        <property type="protein sequence ID" value="AAK42825"/>
        <property type="gene ID" value="SSO2714"/>
    </dbReference>
    <dbReference type="GeneID" id="1452744"/>
    <dbReference type="KEGG" id="sso:SSO2714"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2796"/>
    <dbReference type="eggNOG" id="arCOG00157">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_496634_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VC0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050901">
        <property type="entry name" value="BP-dep_ABC_trans_perm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32243">
        <property type="entry name" value="MALTOSE TRANSPORT SYSTEM PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32243:SF50">
        <property type="entry name" value="MALTOSE_MALTODEXTRIN TRANSPORT SYSTEM PERMEASE PROTEIN MALG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAK42825.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="548" mass="60760" checksum="975D0636BE3D9D9E" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VC8</accession>
    <name>Q97VC8_SACS2</name>
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        <submittedName>
            <fullName evidence="9">Permease, multidrug efflux</fullName>
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        <name evidence="9" type="ordered locus">SSO2704</name>
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        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
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    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
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            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAK42816.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="567" mass="61049" checksum="33F540B2F983E65F" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VK5</accession>
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        <submittedName>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
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                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
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            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
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    <dbReference type="GO" id="GO:0015833">
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
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    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="695"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="Solute-binding protein family 5" evidence="5">
        <location>
            <begin position="86"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="726" mass="80418" checksum="F8F603535A17ABF2" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VK9</accession>
    <name>Q97VK9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Dipeptide ABC transporter ATP binding protein (DppF-3)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dppF-3</name>
        <name evidence="6" type="ordered locus">SSO2615</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90434">
        <property type="entry name" value="H90434"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989337.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VK9"/>
    <dbReference type="FunCoup" id="Q97VK9">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2615"/>
    <dbReference type="PaxDb" id="273057-SSO2615"/>
    <dbReference type="EnsemblBacteria" id="AAK42735">
        <property type="protein sequence ID" value="AAK42735"/>
        <property type="gene ID" value="SSO2615"/>
    </dbReference>
    <dbReference type="GeneID" id="1454062"/>
    <dbReference type="KEGG" id="sso:SSO2615"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2696"/>
    <dbReference type="eggNOG" id="arCOG00184">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="InParanoid" id="Q97VK9"/>
    <dbReference type="PhylomeDB" id="Q97VK9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000016">
        <property type="entry name" value="Oligopeptide ABC transporter ATP-binding component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050319">
        <property type="entry name" value="ABC_transp_ATP-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776:SF7">
        <property type="entry name" value="D,D-DIPEPTIDE TRANSPORT ATP-BINDING PROTEIN DDPF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776">
        <property type="entry name" value="TRANSPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="7"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="323" mass="36447" checksum="241AA2757775092F" modified="2001-10-01" version="1">MSDSILYKAININKYYLAKKRGILESLAREPPIYVRALDNVSVEIRKGEVLGVVGESGSGKTTLGKVLATLEKPTGGNLIFMGMEVNDRNINHVRKHIHMVFQNPMTSINPRMKVKDIVREAMKEKREERVKKLLEEVGLDYNYVKDKYPRELSGGQTQRVAIARALAKEPDFLILDEPTSALDASVQAQILNLLVDLQKERKLTYLFITHNIAVARYISDRVIIMYAGKIVEEGNTKEVISEPMHPYTQSLLSSVPELGKKELKPPSGEVPSLINPPSGCRFNPRCPFAMSVCKEKEPPLVEINGRKVACWLYETNWKKASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VM2</accession>
    <name>Q97VM2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter, ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2600</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90433">
        <property type="entry name" value="C90433"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VM2"/>
    <dbReference type="FunCoup" id="Q97VM2">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2600"/>
    <dbReference type="PaxDb" id="273057-SSO2600"/>
    <dbReference type="EnsemblBacteria" id="AAK42722">
        <property type="protein sequence ID" value="AAK42722"/>
        <property type="gene ID" value="SSO2600"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2600"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2681"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q97VM2"/>
    <dbReference type="PhylomeDB" id="Q97VM2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051782">
        <property type="entry name" value="ABC_Transporter_VariousFunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939:SF1">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="10"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="249" mass="27630" checksum="D339A197D49B47BB" modified="2001-10-01" version="1">MVNLYYIMLLNVRNLTVRYGSFIAVNSLNFSVNSEVYCLLGPNGAGKSSTLKAIMDMVPFEGNIEILGKSNKEKFVKNYVGYVPEQPALYEYMTPSEIISFVASLRGINDLNRINALVKAFSLEMFMNTPIASLSMGNRQKVSILLSLIHEPKLLILDEPFNALDVLSVKVLKELIQSHVKNGGGVLFSTHIMEVAEKICNRIGIMNKGIMVMETSSDNIREAGRSLEDVFLSVTGLDETIKDILKGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VN8</accession>
    <name>Q97VN8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90431">
        <property type="entry name" value="B90431"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992194.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VN8"/>
    <dbReference type="PaxDb" id="273057-SSO2579"/>
    <dbReference type="EnsemblBacteria" id="AAK42705">
        <property type="protein sequence ID" value="AAK42705"/>
        <property type="gene ID" value="SSO2579"/>
    </dbReference>
    <dbReference type="GeneID" id="1454032"/>
    <dbReference type="KEGG" id="sso:SSO2579"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2662"/>
    <dbReference type="eggNOG" id="arCOG02221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135601_2_0_2"/>
    <dbReference type="InParanoid" id="Q97VN8"/>
    <dbReference type="PhylomeDB" id="Q97VN8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18689">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677:SF4">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="4"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="126" mass="14447" checksum="43D648D82D954D28" modified="2001-10-01" version="1">MRKYILDANIIFQFFSGSPNVKKYLLNNDEKFVNAVNLSEFAYHYARKFGLRASSVKLNYLLTFINLVEIDKTLAEIASKIRLKYNLSLDDSFLIATAEVIEGIVVASDHELAERTAKDYNVEFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VP4</accession>
    <name>Q97VP4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2572</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90430">
        <property type="entry name" value="D90430"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991789.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VP4"/>
    <dbReference type="STRING" id="273057.SSO2572"/>
    <dbReference type="PaxDb" id="273057-SSO2572"/>
    <dbReference type="EnsemblBacteria" id="AAK42699">
        <property type="protein sequence ID" value="AAK42699"/>
        <property type="gene ID" value="SSO2572"/>
    </dbReference>
    <dbReference type="GeneID" id="1454026"/>
    <dbReference type="KEGG" id="sso:SSO2572"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2655"/>
    <dbReference type="eggNOG" id="arCOG07326">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369550_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VP4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="199" mass="23170" checksum="F97D2E0A10D74D39" modified="2001-10-01" version="1">MRLKITSIEDLFIPPLQEYSYLCNGIITDMKCKGMEIFRDPDFITFTVNDILSSMSLQGLIKMKTRGRKRERWLRYISKYKMELEPKEFSTILRLGALLTIYVDGYEIEGSQGDVVVKEFRVSGTGSNTEHVKKMLLELSPRLVVIQNKNNIWYIVTGYKVTFVDSQLKKLEKLLVSSDRMECNEIQEEYNTRICVNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VW4</accession>
    <name>Q97VW4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Maleylacetate reductase (ClcE)</fullName>
            <ecNumber evidence="4">1.3.1.32</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">clcE</name>
        <name evidence="4" type="ordered locus">SSO2490</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.3.1.32" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90421">
        <property type="entry name" value="C90421"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923894.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VW4"/>
    <dbReference type="FunCoup" id="Q97VW4">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2490"/>
    <dbReference type="PaxDb" id="273057-SSO2490"/>
    <dbReference type="DNASU" id="1453953"/>
    <dbReference type="EnsemblBacteria" id="AAK42626">
        <property type="protein sequence ID" value="AAK42626"/>
        <property type="gene ID" value="SSO2490"/>
    </dbReference>
    <dbReference type="GeneID" id="1453953"/>
    <dbReference type="KEGG" id="sso:SSO2490"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2571"/>
    <dbReference type="eggNOG" id="arCOG00984">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_846272_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VW4"/>
    <dbReference type="PhylomeDB" id="Q97VW4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018506">
        <property type="term" value="F:maleylacetate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08192">
        <property type="entry name" value="MAR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056798">
        <property type="entry name" value="ADH_Fe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496:SF83">
        <property type="entry name" value="HYDROXYACID-OXOACID TRANSHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25137">
        <property type="entry name" value="ADH_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA" evidence="2">
        <location>
            <begin position="10"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Fe-containing alcohol dehydrogenase-like C-terminal" evidence="3">
        <location>
            <begin position="151"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="327" mass="36353" checksum="CFEA94A43E660983" modified="2001-10-01" version="1">MFKVEYPITQVIYGLNALDWLASVKGKRIALVTTRSLLKSKILEQILSLINAEVIEGPRQHTPVGDVNNLTERLKGYDVVIGLGGGSVIDGIKLSFNGYYIAIPTTFSGAEHTRSGGATIDGIKKSRIGKEADAIILDPRATLETPKWLLIASGVRAIDHAVEALYSKDSTPFTDSLAIEGYKKLVKCIRDLDSIENRALCQIGTWLSSLTMRYAKMGISHNFGYVYGPRFNIPHGVTSCISLPSTIKLNYSVARNKLREIENEGEPLYEFMDKFLKDIGAKRRLSEFTTLDEALKYVQTFIQIVNNSGNPVKIDIETAKRFIEEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VW5</accession>
    <name>Q97VW5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90421">
        <property type="entry name" value="B90421"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923893.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VW5"/>
    <dbReference type="STRING" id="273057.SSO2489"/>
    <dbReference type="PaxDb" id="273057-SSO2489"/>
    <dbReference type="DNASU" id="1453952"/>
    <dbReference type="EnsemblBacteria" id="AAK42625">
        <property type="protein sequence ID" value="AAK42625"/>
        <property type="gene ID" value="SSO2489"/>
    </dbReference>
    <dbReference type="GeneID" id="7797382"/>
    <dbReference type="KEGG" id="sso:SSO2489"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2570"/>
    <dbReference type="eggNOG" id="arCOG03856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1122664_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VW5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="254" mass="28801" checksum="471DEE82BFF45807" modified="2001-10-01" version="1">MIKRLTISPLFLILSLIAYYLSRNDILLYSTLSTILFWTFIILILPTSFYNYIKDGIVKYCKKKIFWFTVGLYISYHVILYSFFFYLLLPGSIKNISFWFSFNAGIGYSIPPPITYFLEWVSNSPAIWFFIGPYEGDVIPFSTFLGVVLALLIGLNVMKILELWGISNKLSKSIVLIPSLGVVSGSSCCVFLPSIVMYSIALSISSITATILSILSSFAYFILAYYALPIVSAIVLFHNLTMLNRLIKKLSFTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W31</accession>
    <name>Q97W31_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2411</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90412">
        <property type="entry name" value="H90412"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989419.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W31"/>
    <dbReference type="STRING" id="273057.SSO2411"/>
    <dbReference type="PaxDb" id="273057-SSO2411"/>
    <dbReference type="EnsemblBacteria" id="AAK42559">
        <property type="protein sequence ID" value="AAK42559"/>
        <property type="gene ID" value="SSO2411"/>
    </dbReference>
    <dbReference type="GeneID" id="1453886"/>
    <dbReference type="KEGG" id="sso:SSO2411"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2494"/>
    <dbReference type="eggNOG" id="arCOG05950">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1639999_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W31"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="167" mass="19400" checksum="443A41BE9116BCD8" modified="2001-10-01" version="1">MNGDAQSYLMERISIALPILEISVPCNTTCVMMTKYKHLLSIENFKAQLEILDSLINLIEDKIYTLRHEIEDKFSQYKANVNIDNLVYAIYKMIEEGGNMILGEKVYFGNKEVAYGEYTVLIGFHNLVEKIVKSDSNIRSLCDEIRYLSESTWEHFDKNIRRSLNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W50</accession>
    <name>Q97W50_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">5'-deoxynucleotidase</fullName>
            <ecNumber evidence="5">3.1.3.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2391</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.1.3.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.89" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90410">
        <property type="entry name" value="D90410"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989449.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W50"/>
    <dbReference type="FunCoup" id="Q97W50">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2391"/>
    <dbReference type="PaxDb" id="273057-SSO2391"/>
    <dbReference type="EnsemblBacteria" id="AAK42539">
        <property type="protein sequence ID" value="AAK42539"/>
        <property type="gene ID" value="SSO2391"/>
    </dbReference>
    <dbReference type="GeneID" id="1453866"/>
    <dbReference type="KEGG" id="sso:SSO2391"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2474"/>
    <dbReference type="eggNOG" id="arCOG04311">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039453_4_2_2"/>
    <dbReference type="InParanoid" id="Q97W50"/>
    <dbReference type="PhylomeDB" id="Q97W50"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000060">
        <property type="entry name" value="Metal dependent phosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845:SF13">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="8">
        <location>
            <begin position="27"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="182" mass="20452" checksum="5EB4B943BD996D25" modified="2001-10-01" version="1">MKLERLLEGAKNLVRTGWMQNGIPSAMGETVASHSFEASILAYVLATELKRKGIEINPEHSALIALFHDAGETLLGDLPKWATSRINKRDAEVEAFDELGIGKELFLELKDLNTNEAKVAKLSDRLSTYLQGLRYKRIGFNVDEIISSYAEEIDKLLSIEPLNQIRELVTRIMNSLNKLNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W65</accession>
    <name>Q97W65_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2372</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90408">
        <property type="entry name" value="D90408"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989469.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W65"/>
    <dbReference type="STRING" id="273057.SSO2372"/>
    <dbReference type="PaxDb" id="273057-SSO2372"/>
    <dbReference type="EnsemblBacteria" id="AAK42523">
        <property type="protein sequence ID" value="AAK42523"/>
        <property type="gene ID" value="SSO2372"/>
    </dbReference>
    <dbReference type="GeneID" id="1453850"/>
    <dbReference type="KEGG" id="sso:SSO2372"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2457"/>
    <dbReference type="eggNOG" id="arCOG05954">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507449_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W65"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="180" mass="21037" checksum="295614F9C13D9ADB" modified="2001-10-01" version="1">MDLIHELKSEYGFDEDEISYALSRARGIIFGFAMEYRARRILQEMNFENIRSVDMPTHDIEAEKDGVKYYVEVKASKKSPTREYSAYKVAMIALLDGVHLTLSMIPKPNLLLTEEILSEPKRILYRFFKLAYLKQLEELKVFLENEKNKEVLDSYSNVIRTISNRYHLSIILDLANPFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W71</accession>
    <name>Q97W71_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdopterin biosynthesis protein (MoeA-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">moeA-2</name>
        <name evidence="4" type="ordered locus">SSO2365</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90407">
        <property type="entry name" value="F90407"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W71"/>
    <dbReference type="FunCoup" id="Q97W71">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2365"/>
    <dbReference type="PaxDb" id="273057-SSO2365"/>
    <dbReference type="EnsemblBacteria" id="AAK42517">
        <property type="protein sequence ID" value="AAK42517"/>
        <property type="gene ID" value="SSO2365"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2365"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2451"/>
    <dbReference type="eggNOG" id="arCOG00217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_7_2_2"/>
    <dbReference type="InParanoid" id="Q97W71"/>
    <dbReference type="PhylomeDB" id="Q97W71"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.10">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.190.11">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011067">
        <property type="entry name" value="PRK14497.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF5">
        <property type="entry name" value="GEPHYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="MoeA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="MoeA N-terminal region -like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="3">
        <location>
            <begin position="187"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="550" mass="61097" checksum="67DFDA03575987DA" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W77</accession>
    <name>Q97W77_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Succinate dehydrogenase subunit C (SdhC)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">sdhC</name>
        <name evidence="3" type="ordered locus">SSO2358</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90406">
        <property type="entry name" value="H90406"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W77"/>
    <dbReference type="FunCoup" id="Q97W77">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2358"/>
    <dbReference type="PaxDb" id="273057-SSO2358"/>
    <dbReference type="EnsemblBacteria" id="AAK42511">
        <property type="protein sequence ID" value="AAK42511"/>
        <property type="gene ID" value="SSO2358"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2358"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2445"/>
    <dbReference type="eggNOG" id="arCOG00338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052147_1_0_2"/>
    <dbReference type="InParanoid" id="Q97W77"/>
    <dbReference type="PhylomeDB" id="Q97W77"/>
    <dbReference type="BRENDA" id="1.3.5.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051278">
        <property type="entry name" value="HdrB/HdrD_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42947">
        <property type="entry name" value="COB--COM HETERODISULFIDE REDUCTASE SUBUNIT B 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42947:SF1">
        <property type="entry name" value="COB--COM HETERODISULFIDE REDUCTASE SUBUNIT B 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cysteine-rich" evidence="2">
        <location>
            <begin position="6"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Cysteine-rich" evidence="2">
        <location>
            <begin position="152"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="293" mass="32094" checksum="0D74DFD84A402ED6" modified="2001-10-01" version="1">MIGMKIAYYPGCATHGLSKDVDIATKKVAEVLGVELVEVPDWNCCGGGFYDEYDEVGHVALNLRNLSQVEKMGLTKMVTPCSVCLHSHRLATYKYKEDKDIKRKTDKRLEGTSVKYEGKVDAEHIVWVLLRDVGLENIKKHVKKPLTALKVGTYYGCQMLRPEQIMGFEKAYNPSSLSELVAVTGATPVPFPAMTSCCGFPLVGSNPKGALKLAFNVLNSAKQSGADLVIHPCSLCHLQLDSLQLKVKAEFNIGWTMPAIYVTQLLGLSFGFSPEELGIGKLAIEVLRSKGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WB1</accession>
    <name>Q97WB1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2324</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90402">
        <property type="entry name" value="F90402"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992498.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WB1"/>
    <dbReference type="STRING" id="273057.SSO2324"/>
    <dbReference type="PaxDb" id="273057-SSO2324"/>
    <dbReference type="DNASU" id="1453804"/>
    <dbReference type="EnsemblBacteria" id="AAK42477">
        <property type="protein sequence ID" value="AAK42477"/>
        <property type="gene ID" value="SSO2324"/>
    </dbReference>
    <dbReference type="GeneID" id="1453804"/>
    <dbReference type="KEGG" id="sso:SSO2324"/>
    <dbReference type="eggNOG" id="arCOG07316">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138957_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WB1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="161" mass="18269" checksum="8613BAC7C2FC008A" modified="2001-10-01" version="1">MAPIFLEYKVVHDNITYYIIYVFIYNLSLKLVTMYTINPSSHTILNYNVSLEPEQIFSNITGVSTYYSLDGKKILAYEDNNGSIIVAYNGLILKKASNNSVRYLIAANYPGIDNSKFPSLYKLAEISIFDSNIRVLVLGVLLTILSSFILKVMVGRYDKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WD1</accession>
    <name>Q97WD1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cobalamin biosynthesis precorrin-6B methylase, putative (CbiE)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cbiE</name>
        <name evidence="7" type="ordered locus">SSO2296</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90400">
        <property type="entry name" value="B90400"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991815.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WD1"/>
    <dbReference type="FunCoup" id="Q97WD1">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2296"/>
    <dbReference type="PaxDb" id="273057-SSO2296"/>
    <dbReference type="EnsemblBacteria" id="AAK42457">
        <property type="protein sequence ID" value="AAK42457"/>
        <property type="gene ID" value="SSO2296"/>
    </dbReference>
    <dbReference type="GeneID" id="1453784"/>
    <dbReference type="KEGG" id="sso:SSO2296"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2389"/>
    <dbReference type="eggNOG" id="arCOG00650">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089162_1_0_2"/>
    <dbReference type="InParanoid" id="Q97WD1"/>
    <dbReference type="PhylomeDB" id="Q97WD1"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11644">
        <property type="entry name" value="Precorrin-6Y-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012818">
        <property type="entry name" value="CbiE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050714">
        <property type="entry name" value="Cobalamin_biosynth_MTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02467">
        <property type="entry name" value="CbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004455">
        <property type="entry name" value="PRK05787.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182">
        <property type="entry name" value="COBALT-PRECORRIN-6B C(15)-METHYLTRANSFERASE (DECARBOXYLATING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-7 C(5)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="6">
        <location>
            <begin position="7"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="227" mass="25549" checksum="0267F09491F2DCED" modified="2001-10-01" version="1">MRVSKITVYIVGVGPGDPEYLTLKGYKAIRDSVVVAGWKSVLERFSPILEGKRTVVLTYKNESETLEEVIEIGKTENVAILDHGDPSVSDWQFVEKIKNIAASKGVKVNIISGVSSLNIALSRLGLDINFIGFVTLHVRGDISKFLNDILNILRMGRVAIVIPEPHKDGPQKVAKFLYDNNLNCRIVVFEKLSYDDEKRRVYDNLVSLMNEKNDFSDLTMMVIFPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WI9</accession>
    <name>Q97WI9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2227</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90392">
        <property type="entry name" value="F90392"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923808.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WI9"/>
    <dbReference type="PaxDb" id="273057-SSO2227"/>
    <dbReference type="EnsemblBacteria" id="AAK42397">
        <property type="protein sequence ID" value="AAK42397"/>
        <property type="gene ID" value="SSO2227"/>
    </dbReference>
    <dbReference type="GeneID" id="1453724"/>
    <dbReference type="KEGG" id="sso:SSO2227"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2323"/>
    <dbReference type="eggNOG" id="arCOG03765">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1406031_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WI9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="191" mass="21213" checksum="9889DF0B8037B43D" modified="2001-10-01" version="1">MPRVNIAAEADLIEQLENEAKKRGYTIYSLTNAALKALLKLLKEGEDANTLESLVDYYTISKALDIVPVTSWFLENLTKLAYEKDNKQYENMCEEVGEQIGSFLRSKASTLDELFDFYNAIKIALPIRNVSIKNTGDMIEFRITGTGFSLISTLCAGKIFSKIAEAYDLSIQDVDVVPGGIVTIKAKANLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WK3</accession>
    <name>Y2213_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0284 protein SSO2213</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2213</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0284 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90390">
        <property type="entry name" value="H90390"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992069.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WK3"/>
    <dbReference type="SMR" id="Q97WK3"/>
    <dbReference type="STRING" id="273057.SSO2213"/>
    <dbReference type="PaxDb" id="273057-SSO2213"/>
    <dbReference type="EnsemblBacteria" id="AAK42383">
        <property type="protein sequence ID" value="AAK42383"/>
        <property type="gene ID" value="SSO2213"/>
    </dbReference>
    <dbReference type="GeneID" id="1453710"/>
    <dbReference type="KEGG" id="sso:SSO2213"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2309"/>
    <dbReference type="eggNOG" id="arCOG04272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053134_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WK3"/>
    <dbReference type="PhylomeDB" id="Q97WK3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008939">
        <property type="term" value="F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02439">
        <property type="entry name" value="DMB-PRT_CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01086">
        <property type="entry name" value="UPF0284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003200">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002805">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036087">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00303">
        <property type="entry name" value="nicotinate mononucleotide-dependent phosphoribosyltransferase CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003368">
        <property type="entry name" value="PRK04447.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003370">
        <property type="entry name" value="PRK04447.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003372">
        <property type="entry name" value="PRK04447.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811:SF1">
        <property type="entry name" value="UPF0284 PROTEIN SLL1500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02277">
        <property type="entry name" value="DBI_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52733">
        <property type="entry name" value="Nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (CobT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151061" description="UPF0284 protein SSO2213">
        <location>
            <begin position="1"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01086"/>
        </source>
    </evidence>
    <sequence length="347" mass="36980" checksum="88B69679EBA07A4B" modified="2001-10-01" version="1">MIKAYFGAETFTLNKDFAYILVIGTTDVSLIPGLTIAGATPELTHFTPAADAEYVILGKCKSINTIPVSPTGIPTPALLTRASLSFTKSLKIVVNAGSRITPKIPYIDLQGEPGKDIRKQALSIEKVNIIIENGIKLGEELSNEYELIMIGESIPAGTTTAMATLLALGYDAMDKVSSASPDNPKELKRKVVEEALRNLPTDPLQRLAKVSDPVLLGVAGISLGFRGKILLAGGTQMTAAAAIVNEFDKSKLKDIIIGTTKWIVEDKSADMLSLAKQVGVKVLASMLDLSISIHEGIRTYEKGYVKEGVGAGGSTIMAFIRGVSNSTLVRKIDELYSELVGSNNLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WK7</accession>
    <name>Q97WK7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">SSO2209</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90390">
        <property type="entry name" value="D90390"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992076.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WK7"/>
    <dbReference type="STRING" id="273057.SSO2209"/>
    <dbReference type="CAZy" id="GT21">
        <property type="family name" value="Glycosyltransferase Family 21"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2209"/>
    <dbReference type="DNASU" id="1453706"/>
    <dbReference type="EnsemblBacteria" id="AAK42379">
        <property type="protein sequence ID" value="AAK42379"/>
        <property type="gene ID" value="SSO2209"/>
    </dbReference>
    <dbReference type="GeneID" id="1453706"/>
    <dbReference type="KEGG" id="sso:SSO2209"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2305"/>
    <dbReference type="eggNOG" id="arCOG01389">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067021_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WK7"/>
    <dbReference type="PhylomeDB" id="Q97WK7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008120">
        <property type="term" value="F:ceramide glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006679">
        <property type="term" value="P:glucosylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000232">
        <property type="entry name" value="Glycosyl transferase family 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025993">
        <property type="entry name" value="Ceramide_glucosylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726:SF0">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="10">
        <location>
            <begin position="42"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK42379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="352" mass="41010" checksum="0DC4BB0CF33EBC83" modified="2001-10-01" version="1">MEYYNLILYLLVFGADMLIILQTYNEIKMWKTVKGNFVGKVSIIVPIKGIDTRLERNIESLLSQDYPYPYEVIYVVEKGDEVENILKKYNVKIVYSDFNCDSCSGKIRAQISGLLRASNDIIVFADSDTWYPRYWLRNLISPLDKYDTTTTFSWPFPSRITLSNLIRAGFWTLGFESQSLQSSRFLWGGSMALRREFFDNEVLDELSKEWCDDCTLTRIIKRRGGKIGFVMSAIPLNVYDENSLISWSSRQIITILAYSPRGARAYLVAGTFFALILIYSLVYLNIVTFTPYILWIVKNVIRGVKYPKQALIASLMTILAIPYALFLLVFNWNRREVYWRGKRYIVKPLREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WL3</accession>
    <name>Q97WL3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90389">
        <property type="entry name" value="F90389"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WL3"/>
    <dbReference type="STRING" id="273057.SSO2203"/>
    <dbReference type="PaxDb" id="273057-SSO2203"/>
    <dbReference type="EnsemblBacteria" id="AAK42373">
        <property type="protein sequence ID" value="AAK42373"/>
        <property type="gene ID" value="SSO2203"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2203"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2299"/>
    <dbReference type="eggNOG" id="arCOG07715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085262_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WL3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="284" mass="31370" checksum="983D17C8185399A9" modified="2001-10-01" version="1">MFSYVLANGKCNFVYYISETTLHHASVKVGFLIYCNHCFQLLLSLTLPCLLDIKHLVIVSKGAVILILITLFVYTLNIISNAYLVNVKILSPFPITLMFSDSQGVKLVSSNQTLNVSSSNLRIQVFVLSPYSYCIFINGNKTDEFNVTLSNYSQFNLTVTVIPKYAFLKVNVVGKGTAYIELDNGSIIAVRNSSVIRVYNGSSVLLEANGDLINWSNGANTMTLWYTVGGNSTITAFFGNSSILPKSGTVKPTVNYTEVGLSLFAVGFFILYKIYSRKDQDTNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WM4</accession>
    <name>Q97WM4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90388">
        <property type="entry name" value="C90388"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992108.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WM4"/>
    <dbReference type="STRING" id="273057.SSO2190"/>
    <dbReference type="PaxDb" id="273057-SSO2190"/>
    <dbReference type="EnsemblBacteria" id="AAK42362">
        <property type="protein sequence ID" value="AAK42362"/>
        <property type="gene ID" value="SSO2190"/>
    </dbReference>
    <dbReference type="GeneID" id="1453689"/>
    <dbReference type="KEGG" id="sso:SSO2190"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2285"/>
    <dbReference type="eggNOG" id="arCOG05883">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1168616_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WM4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="238" mass="27391" checksum="F57C5332C0A76001" modified="2001-10-01" version="1">MKCSTKSIREIRGFSFLVPNCDSTLLREKFESFRKLSNEENKTYIIRIADSNYFKFEVLMLPNSLTLLTISTVRGTNNINLRDFEMLDELQTLACCAYQKEELKNTTSNFLSPTAQIAEAEVVDLISTDEEFECQIECDNVSWNEIKLPESTEGKWTTTELASTSRLYLKALSGQAKFLTISTERPQNSIKFTSMERLGALCCFLTELKRKLNECPTLRDVLQPYLQVKTGRKRKKNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2026-01-28" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WM8</accession>
    <name>CDC63_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ORC1-type DNA replication protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cdc6-3</name>
        <name type="ordered locus">SSO2184</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="Extremophiles" volume="10" first="61" last="70">
            <title>Biochemical characterization of two Cdc6/ORC1-like proteins from the crenarchaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="De Felice M."/>
                <person name="Esposito L."/>
                <person name="Rossi M."/>
                <person name="Pisani F.M."/>
            </authorList>
            <dbReference type="PubMed" id="16179962"/>
            <dbReference type="DOI" id="10.1007/s00792-005-0473-0"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>DNA-BINDING</scope>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH CDC6-1 AND CDC6-2</scope>
        <scope>LACK OF AUTOPHOSPHORYLATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2007" name="Biochem. Biophys. Res. Commun." volume="361" first="651" last="658">
            <title>Divergent functions of multiple eukaryote-like Orc1/Cdc6 proteins on modulating the loading of the MCM helicase onto the origins of the hyperthermophilic archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Jiang P.X."/>
                <person name="Wang J."/>
                <person name="Feng Y."/>
                <person name="He Z.G."/>
            </authorList>
            <dbReference type="PubMed" id="17673179"/>
            <dbReference type="DOI" id="10.1016/j.bbrc.2007.07.073"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH MCM</scope>
        <scope>DNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2007" name="Biochem. Biophys. Res. Commun." volume="363" first="63" last="70">
            <title>Three eukaryote-like Orc1/Cdc6 proteins functionally interact and mutually regulate their activities of binding to the replication origin in the hyperthermophilic archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Wang J."/>
                <person name="Jiang P.X."/>
                <person name="Feng H."/>
                <person name="Feng Y."/>
                <person name="He Z.G."/>
            </authorList>
            <dbReference type="PubMed" id="17825793"/>
            <dbReference type="DOI" id="10.1016/j.bbrc.2007.08.125"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH CDC6-1 AND CDC6-2</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2008" name="J. Virol." volume="82" first="4874" last="4883">
            <title>Transcriptome analysis of infection of the archaeon Sulfolobus solfataricus with Sulfolobus turreted icosahedral virus.</title>
            <authorList>
                <person name="Ortmann A.C."/>
                <person name="Brumfield S.K."/>
                <person name="Walther J."/>
                <person name="McInnerney K."/>
                <person name="Brouns S.J."/>
                <person name="van de Werken H.J."/>
                <person name="Bothner B."/>
                <person name="Douglas T."/>
                <person name="van de Oost J."/>
                <person name="Young M.J."/>
            </authorList>
            <dbReference type="PubMed" id="18337566"/>
            <dbReference type="DOI" id="10.1128/jvi.02583-07"/>
        </citation>
        <scope>INDUCTION BY VIRUS (MICROBIAL INFECTION)</scope>
        <source>
            <strain>2-2-12</strain>
        </source>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2009" name="Proc. Natl. Acad. Sci. U.S.A." volume="106" first="7792" last="7797">
            <title>Archaeal eukaryote-like Orc1/Cdc6 initiators physically interact with DNA polymerase B1 and regulate its functions.</title>
            <authorList>
                <person name="Zhang L."/>
                <person name="Zhang L."/>
                <person name="Liu Y."/>
                <person name="Yang S."/>
                <person name="Gao C."/>
                <person name="Gong H."/>
                <person name="Feng Y."/>
                <person name="He Z.G."/>
            </authorList>
            <dbReference type="PubMed" id="19416914"/>
            <dbReference type="DOI" id="10.1073/pnas.0813056106"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH POLB1</scope>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2007" name="Science" volume="317" first="1210" last="1213">
            <title>Replication origin recognition and deformation by a heterodimeric archaeal Orc1 complex.</title>
            <authorList>
                <person name="Dueber E.L."/>
                <person name="Corn J.E."/>
                <person name="Bell S.D."/>
                <person name="Berger J.M."/>
            </authorList>
            <dbReference type="PubMed" id="17761879"/>
            <dbReference type="DOI" id="10.1126/science.1143690"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.35 ANGSTROMS) OF 14-394 IN COMPLEX WITH CDC6-1 AND DNA</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 3 5 7">Involved in regulation of DNA replication. May play essential roles in origin recognition and cell cycle control of replication. Binds to DNA, with a preference for molecules that contain a bubble, a fork, or a tail. Inhibits the binding of the MCM helicase to the origin DNA and inhibits its DNA helicase activity. Also regulates the DNA polymerase and the nuclease activities of PolB1. Inhibits the DNA-binding activity of Cdc6-1 and Cdc6-2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3 4 5 7">Monomer. Interacts with Cdc6-1, Cdc6-2, MCM and PolB1.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-9026919">
            <id>Q97WM8</id>
        </interactant>
        <interactant intactId="EBI-15778666">
            <id>P26811</id>
            <label>dpo1</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>3</experiments>
    </comment>
    <comment type="induction">
        <text evidence="6">(Microbial infection) At least 4-fold induced following infection by Sulfolobus turreted icosahedral virus 1 (STIV-1) in strain 2-2-12.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">Strain 2-2-12 is a substrain of P2 that is highly susceptible to infection by Sulfolobus turreted icosahedral virus 1 (STIV-1).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDC6/cdc18 family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAK42358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90387">
        <property type="entry name" value="G90387"/>
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    <dbReference type="RefSeq" id="WP_009992115.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="PDB" id="2QBY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.35 A"/>
        <property type="chains" value="B=14-394"/>
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    <dbReference type="PDBsum" id="2QBY"/>
    <dbReference type="AlphaFoldDB" id="Q97WM8"/>
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    <dbReference type="DIP" id="DIP-48853N"/>
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        <property type="interactions" value="85"/>
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        <property type="interactions" value="1"/>
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    <dbReference type="STRING" id="273057.SSO2184"/>
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    <dbReference type="EnsemblBacteria" id="AAK42358">
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        <property type="gene ID" value="SSO2184"/>
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    <dbReference type="PATRIC" id="fig|273057.12.peg.2280"/>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="HLD_clamp"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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    </dbReference>
    <dbReference type="HAMAP" id="MF_01407">
        <property type="entry name" value="ORC1_type_DNA_replic_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_22"/>
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        <property type="entry name" value="Cdc6_C"/>
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        <property type="entry name" value="Cdc6_lid"/>
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        <property type="entry name" value="ORC1/CDC6"/>
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        <property type="entry name" value="Orc1/Cdc6_arc"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="orc1/cdc6 family replication initiation protein"/>
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        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
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        <property type="entry name" value="WHD_Cdc6"/>
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        <property type="entry name" value="Cdc6_C"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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    </feature>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WN5</accession>
    <name>Q97WN5_SACS2</name>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90386">
        <property type="entry name" value="H90386"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992125.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WN5"/>
    <dbReference type="PaxDb" id="273057-SSO2176"/>
    <dbReference type="EnsemblBacteria" id="AAK42351">
        <property type="protein sequence ID" value="AAK42351"/>
        <property type="gene ID" value="SSO2176"/>
    </dbReference>
    <dbReference type="GeneID" id="1453678"/>
    <dbReference type="KEGG" id="sso:SSO2176"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2272"/>
    <dbReference type="eggNOG" id="arCOG07324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1465179_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WN5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="184" mass="21050" checksum="555DF2EEED2172D7" modified="2001-10-01" version="1">MVYQLAFIFLILLISIPLSPHQSFSYNVQIIYNNTLYLYVYNYTILSISPLTYNFTIYNSNGSIIYNKVFTISNYSLFPPALLINASLIGNYTLILSKMENNTNISVYKGFLKLNRSEIVLNLIYHNNILYQANGTSKNVHIYIFQINSENESPSPTIYSYLPLIVLLIVIIVAVILLIKIGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WY5</accession>
    <name>Q97WY5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1975</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90363">
        <property type="entry name" value="G90363"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989379.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WY5"/>
    <dbReference type="STRING" id="273057.SSO1975"/>
    <dbReference type="PaxDb" id="273057-SSO1975"/>
    <dbReference type="EnsemblBacteria" id="AAK42166">
        <property type="protein sequence ID" value="AAK42166"/>
        <property type="gene ID" value="SSO1975"/>
    </dbReference>
    <dbReference type="GeneID" id="7806434"/>
    <dbReference type="KEGG" id="sso:SSO1975"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2051"/>
    <dbReference type="eggNOG" id="arCOG02219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118482_3_5_2"/>
    <dbReference type="InParanoid" id="Q97WY5"/>
    <dbReference type="PhylomeDB" id="Q97WY5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09881">
        <property type="entry name" value="PIN_VapC4-5_FitB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050556">
        <property type="entry name" value="Type_II_TA_system_RNase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33653">
        <property type="entry name" value="RIBONUCLEASE VAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33653:SF1">
        <property type="entry name" value="RIBONUCLEASE VAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="7">
        <location>
            <begin position="3"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="125" mass="14134" checksum="68CC09D109BAFFEE" modified="2001-10-01" version="1">MKVLIESSAIIEYLKGNAKVKEIISNSEDFYVSTLTIFEVLLGKVEENKILDFLSAFNVIGLNKKDSIIASRIYKRLRDKGKLIGYFDILISAQAINRDLTLVTKDTDFLKVADEFNELKVALIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X16</accession>
    <name>Q97X16_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial transposase in ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90359">
        <property type="entry name" value="E90359"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X16"/>
    <dbReference type="PaxDb" id="273057-SSO1937"/>
    <dbReference type="EnsemblBacteria" id="AAK42132">
        <property type="protein sequence ID" value="AAK42132"/>
        <property type="gene ID" value="SSO1937"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1937"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2012"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754799_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X16"/>
    <dbReference type="PhylomeDB" id="Q97X16"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="148" mass="16746" checksum="24D0FBCBB989148E" modified="2001-10-01" version="1">MLGILRLANLSDLAFSFDGFKLLADRASRKNILVKGVSSARLPVEGSGIKIREGKASATTLRGPIESSYRNIKAFLPFTGSTKFVFRTLIFVLALVLYSLYTIFKGEVGREEFRLLLILLFPDLFNPENFTFNVIETLIYTIDLFLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-10-02" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X36</accession>
    <name>Q97X36_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1395</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1919</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99357">
        <property type="entry name" value="A99357"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X36"/>
    <dbReference type="STRING" id="273057.SSO1919"/>
    <dbReference type="PaxDb" id="273057-SSO1919"/>
    <dbReference type="EnsemblBacteria" id="AAK42112">
        <property type="protein sequence ID" value="AAK42112"/>
        <property type="gene ID" value="SSO1919"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1919"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1984"/>
    <dbReference type="eggNOG" id="arCOG02127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172291_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X36"/>
    <dbReference type="PhylomeDB" id="Q97X36"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13551">
        <property type="entry name" value="HTH_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="108" mass="12777" checksum="6490AC8390D7BB4A" modified="2001-10-01" version="1">MLVMEAVGLSVLRTLKREIRKLISKKPKNLNETKARAILLHLEGMKISEIAKILQVHKSTVYRWINEFEKEGEKCLFYKQRKGRKKKVNEREIKVEEIQGKTIWEAKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X75</accession>
    <name>Q97X75_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thermopsin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90351">
        <property type="entry name" value="D90351"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X75"/>
    <dbReference type="PaxDb" id="273057-SSO1878"/>
    <dbReference type="EnsemblBacteria" id="AAK42067">
        <property type="protein sequence ID" value="AAK42067"/>
        <property type="gene ID" value="SSO1878"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1878"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1930"/>
    <dbReference type="eggNOG" id="arCOG03673">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058361_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X75"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="347"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="41776" checksum="4AB76672BF79AC9B" modified="2001-10-01" version="1">MLLMKVITLFLFLFIILIPIVNAGYDYGYEFGYVNMNGIQAIVTLYNISLQPGSPPISIQQNVCLNLNGSEILVQNVIIPLILTATNQYEINWVNSVFYNGSYHTFSYSFVVGNTFNLTTVWVNSSDKLVITFYLSNCSTSLNRTYVLYGNYIGVVYSGYDAGTVIVGFRNGATADFVKGFNVSIQEFYRYDNSWYVPPVAYSGYSNTGESATNGYAYFYDGKVWVIYGNASTQMLYNFSVIIVNNTIFTFPKGSLWLSDGNFFTDSIPLKGNLIISPFYYFNYSFSTKKQILLKFNNYTVVDGIKGKAFYLPSSENVYIRENGKCVMEFINQSNVNQREGANPMKLLFVIVVIIAGILSLLYYKMIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XA5</accession>
    <name>Q97XA5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glyceraldehyde-3-phosphate dehydrogenase, NADP dependent (GapN-2)</fullName>
            <ecNumber evidence="6">1.2.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">gapN-2</name>
        <name evidence="6" type="ordered locus">SSO1842</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.9" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90347">
        <property type="entry name" value="F90347"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XA5"/>
    <dbReference type="FunCoup" id="Q97XA5">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1842"/>
    <dbReference type="PaxDb" id="273057-SSO1842"/>
    <dbReference type="EnsemblBacteria" id="AAK42037">
        <property type="protein sequence ID" value="AAK42037"/>
        <property type="gene ID" value="SSO1842"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1842"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1901"/>
    <dbReference type="eggNOG" id="arCOG01252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_0_2"/>
    <dbReference type="InParanoid" id="Q97XA5"/>
    <dbReference type="PhylomeDB" id="Q97XA5"/>
    <dbReference type="BRENDA" id="1.2.1.79">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="1.2.1.9">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008886">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (non-phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008911">
        <property type="term" value="F:lactaldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07145">
        <property type="entry name" value="ALDH_LactADH_F420-Bios"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000018">
        <property type="entry name" value="Alpha-aminoadipic semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000007">
        <property type="entry name" value="NAD/NADP-dependent betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051020">
        <property type="entry name" value="ALDH-related_metabolic_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42991">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42991:SF1">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="5"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="470" mass="51604" checksum="6C22E206D5B8326C" modified="2001-10-01" version="1">MMSLEVIEVRSPSNLKVIGTVKRMSKDEVRGEIEEAYKGFEIISRMPLYKRTAILRKISEILEREQERLARLLAMEAGKPISDSRVEVLRASRLFRQAAEEVGIVLEGKNYRVDAYEYPPGNENRIVLSTREPIGVVTAILPFNFPINSFAHKVAPALAVGNSVVVKPSINTPLAAVEMKKILVEAGLPDSAVRVVTGYSSEIGDEIITHPLVGLITLTGSTQTGLKIASKAISLGKRIIMELGGSDPIIILEDANIERASSIAVRARFEYAGQNCNAGKRIIVRQEVYDKFVKAFNEKAKALKVGEPLDETTDVGPVINKESVENLNSVLEDAKVKGGRVEILNRGPESGSFFPLTMVTNPSLDMLVLKSEVFGPIVPIVSVKSDEEAIRIANSTEYGLQSAIFTNDVNRALKLSRELKFGAVIINDSTRLRWDSLPFGGFKKTGIGREGVRETMLEMTENKLIAITLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XC4</accession>
    <name>Q97XC4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90345">
        <property type="entry name" value="B90345"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XC4"/>
    <dbReference type="STRING" id="273057.SSO1820"/>
    <dbReference type="PaxDb" id="273057-SSO1820"/>
    <dbReference type="EnsemblBacteria" id="AAK42017">
        <property type="protein sequence ID" value="AAK42017"/>
        <property type="gene ID" value="SSO1820"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1820"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1880"/>
    <dbReference type="eggNOG" id="arCOG05901">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1136103_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XC4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="248" mass="29006" checksum="AB9D01F17F8F8CE3" modified="2001-10-01" version="1">MKSVMNKIDKDEALELYRLTNFINSENLITSLEKRLDNYKLYLANLLYNNYSPNEDLRSALKLLDSLTLSRCQFLIGHKKFLFMEDDINDLLFCYNIKLAKSLIMGNKYDYKITRSDIIELSDIYEDFNFIQNNAIIAKIALGYKEDYLKKHINLTPLALGALALTGDEFHKDIFNSIIESIVEKYGEESLLYTTAMRESYMIYLLLHGKDISKIKINEAYRMDISSVLARKFLLNYKQRYLASLVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XC7</accession>
    <name>Q97XC7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8998</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90344">
        <property type="entry name" value="G90344"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992890.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XC7"/>
    <dbReference type="PaxDb" id="273057-SSO8998"/>
    <dbReference type="EnsemblBacteria" id="AAK42014">
        <property type="protein sequence ID" value="AAK42014"/>
        <property type="gene ID" value="SSO8998"/>
    </dbReference>
    <dbReference type="GeneID" id="1454793"/>
    <dbReference type="KEGG" id="sso:SSO8998"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1877"/>
    <dbReference type="eggNOG" id="arCOG07052">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192646_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XC7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="86" mass="10002" checksum="998E7C183B7A386B" modified="2001-10-01" version="1">MKVIEKIKLDRTESCGSKSPLSIMLDAIRKIKECDESVEILMNDYDWLLSLKYILQVNDKKMKVEEKGSEEHYLKVEVSMDCSQSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XD4</accession>
    <name>Q97XD4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prolidase (Xaa-Pro dipeptidase) (PepQ-like1)</fullName>
            <ecNumber evidence="2">3.4.13.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pepQ-like1</name>
        <name evidence="2" type="ordered locus">SSO1811</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.13.9" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90343">
        <property type="entry name" value="H90343"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991467.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XD4"/>
    <dbReference type="FunCoup" id="Q97XD4">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1811"/>
    <dbReference type="PaxDb" id="273057-SSO1811"/>
    <dbReference type="EnsemblBacteria" id="AAK42007">
        <property type="protein sequence ID" value="AAK42007"/>
        <property type="gene ID" value="SSO1811"/>
    </dbReference>
    <dbReference type="GeneID" id="1454786"/>
    <dbReference type="KEGG" id="sso:SSO1811"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1869"/>
    <dbReference type="eggNOG" id="arCOG00696">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023620_2_2_2"/>
    <dbReference type="InParanoid" id="Q97XD4"/>
    <dbReference type="PhylomeDB" id="Q97XD4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102009">
        <property type="term" value="F:proline dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01299">
        <property type="entry name" value="Met_dep_hydrolase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000161">
        <property type="entry name" value="Amidohydrolase, imidazolonepropionase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051781">
        <property type="entry name" value="Metallo-dep_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057744">
        <property type="entry name" value="OTAase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135:SF3">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="47"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="396" mass="44087" checksum="5E59A62ACDA54FDD" modified="2001-10-01" version="1">MITLVGKIFDGEKIIEQGTVVIENDKIERVSEGIDIPQGSEVIQANFIMPGLVDAHLHFFGVEEDNVLSWNIVNEIDVAIRSTRDMERLLRSGFTLVRDLGSKVAVRLDYLQRRGEILGPTVIASGYSLAITGGNDDPKDLPIDIAQRLSYSFYCDSPYECRKAVRMAIRQGARVIKVYASGAFSQGGKILPGFALDELKAIVDESHRFGLKVASHAYGKEAIMNSILAGVDTIEHGLGLDDETASMIKERGICYIPTLATYEVPFEVRDPEVRIYREELVKRHFSEDIRIAVSHGVKIATGTDYVGSKKRPHGQNYRESVLLSRYMSNLEVLRASTSIASECLGVKVGYIREGYKADLIVLKDDPTRDIENLKPNNVSYVIKDGKLYTGYGLYQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XD5</accession>
    <name>Q97XD5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO1810</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC-4 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90343">
        <property type="entry name" value="G90343"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991468.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XD5"/>
    <dbReference type="STRING" id="273057.SSO1810"/>
    <dbReference type="PaxDb" id="273057-SSO1810"/>
    <dbReference type="EnsemblBacteria" id="AAK42006">
        <property type="protein sequence ID" value="AAK42006"/>
        <property type="gene ID" value="SSO1810"/>
    </dbReference>
    <dbReference type="GeneID" id="1454785"/>
    <dbReference type="KEGG" id="sso:SSO1810"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1868"/>
    <dbReference type="eggNOG" id="arCOG02312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_2"/>
    <dbReference type="InParanoid" id="Q97XD5"/>
    <dbReference type="PhylomeDB" id="Q97XD5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050250">
        <property type="entry name" value="Macrolide_Exporter_MacB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30572:SF4">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE YTRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30572">
        <property type="entry name" value="MEMBRANE COMPONENT OF TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="302"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="MacB-like periplasmic core" evidence="9">
        <location>
            <begin position="22"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="ABC3 transporter permease C-terminal" evidence="8">
        <location>
            <begin position="260"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="41897" checksum="A2F0F68F18461D24" modified="2001-10-01" version="1">MNGVDILWLAYKGLMARKTLAIISIIAIMIGITSVSFIEAFSQGVERSVISTLFQLNPTNIYVFNEIGYVSPTDVSLMSSLPGIDAVYPVIEAHGVVQIGGRVINVLVVGVDNISSLLGKVELENGTVYPPVTVPYAVIGHDIAHPIPNVTVQPGSTIILKLSNGNALPLTVYGILRPTQSVVIGDTSDVIFIPLGEAKALINPPGYFLVVLQGSSISEVNTITTLLNYIYGNSLTVTTIQQAISSVQVIITSFSFLVILIGSISLFVGAVGIMGITLARVYQRTREIGIMKTVGLTTRQVLLVFLLEALIVGVIGGIVGLTLTIMGTYIMDINGIPFNAGSSNGSNLIVVIRPFLSMSDVAISILIALVTSIIAGIYPAWKASKLTVIEAVRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XT8</accession>
    <name>Q97XT8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1619</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90322">
        <property type="entry name" value="C90322"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923521.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XT8"/>
    <dbReference type="STRING" id="273057.SSO1619"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1619"/>
    <dbReference type="DNASU" id="1454613"/>
    <dbReference type="EnsemblBacteria" id="AAK41834">
        <property type="protein sequence ID" value="AAK41834"/>
        <property type="gene ID" value="SSO1619"/>
    </dbReference>
    <dbReference type="GeneID" id="1454613"/>
    <dbReference type="KEGG" id="sso:SSO1619"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1661"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_817896_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XT8"/>
    <dbReference type="PhylomeDB" id="Q97XT8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12526">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12526:SF630">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="341" mass="39355" checksum="1A12353F8BAC65D6" modified="2001-10-01" version="1">MRILISPPMRFGKEFLGGMRRTLEVYSRMGEEVYLCVDERTLPQVDPEISPLLSKFHLVSSSSTDKKTYLKGFLNCLKYARKVDVLISYSEYSLSVYYTWLLSLFSRKPFIVVVHHVTEEIRGSKFLRTVIARSKGIICLDNPEVYEELKSLFPNKVILTSTNGVSVEDYYTSEEKICDGIFVGNYGERKGSRYLFQIWDKVNEKIDAKLCIIGKGWSEIPKNSVYYGFVTEREKIDLLAKSKVFVFPSLYEGFALAVTEALSAYLPVVTWDLKWSERYPVAIRVTYPDISSFAEEVVKLLKDEKLRKSIGEKSRELAKTLNWEKAAEMEKKAIYKIMESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y20</accession>
    <name>Q97Y20_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dihydrolipoamide S-acetyltransferase, amino-end (PdhC)</fullName>
            <ecNumber evidence="5">2.3.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pdhC</name>
        <name evidence="5" type="ordered locus">SSO1529</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.12" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90311">
        <property type="entry name" value="H90311"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y20"/>
    <dbReference type="SMR" id="Q97Y20"/>
    <dbReference type="FunCoup" id="Q97Y20">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1529"/>
    <dbReference type="PaxDb" id="273057-SSO1529"/>
    <dbReference type="EnsemblBacteria" id="AAK41751">
        <property type="protein sequence ID" value="AAK41751"/>
        <property type="gene ID" value="SSO1529"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1529"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1568"/>
    <dbReference type="eggNOG" id="arCOG01706">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_7_2_2"/>
    <dbReference type="InParanoid" id="Q97Y20"/>
    <dbReference type="PhylomeDB" id="Q97Y20"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004742">
        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="entry name" value="E3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045257">
        <property type="entry name" value="E2/Pdx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151:SF90">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="3">
        <location>
            <begin position="2"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="4">
        <location>
            <begin position="114"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="211" mass="23327" checksum="147D9BEEA6B4B931" modified="2001-10-01" version="1">MGKEVLMPKLGLTMTKGKIVQWKKKEGDRVQEGEDLVIIETEKITTTVKSPVSGILLKIYAKEGEEVPVGQIIAYIGEIGEQPPPSPTKPALATQQQQAQPIRTEEVKVIGEVRASPRARRLAKEKGIDLSKIRGTGPGGMITEDDVIRELENIEKGMKFTATGLRVKEVIPMSVIRQEISRRMVQSLQTMAQVTLSIEINANSLVKMKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y68</accession>
    <name>Q97Y68_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2006" name="J. Biol. Chem." volume="281" first="4261" last="4266">
            <title>Crystal structure of a metal ion-bound IS200 transposase.</title>
            <authorList>
                <person name="Lee H.H."/>
                <person name="Yoon J.Y."/>
                <person name="Kim H.S."/>
                <person name="Kang J.Y."/>
                <person name="Kim K.H."/>
                <person name="Kim D.J."/>
                <person name="Ha J.Y."/>
                <person name="Mikami B."/>
                <person name="Yoon H.J."/>
                <person name="Suh S.W."/>
            </authorList>
            <dbReference type="PubMed" id="16340015"/>
            <dbReference type="DOI" id="10.1074/jbc.m511567200"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH MN(2+)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90305">
        <property type="entry name" value="F90305"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992706.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2F4F">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B=1-133"/>
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    <dbReference type="PDB" id="2F5G">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A/B=1-133"/>
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    <dbReference type="PDBsum" id="2F4F"/>
    <dbReference type="PDBsum" id="2F5G"/>
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    <dbReference type="STRING" id="273057.SSO1474"/>
    <dbReference type="PaxDb" id="273057-SSO1474"/>
    <dbReference type="EnsemblBacteria" id="AAK41701">
        <property type="protein sequence ID" value="AAK41701"/>
        <property type="gene ID" value="SSO1474"/>
    </dbReference>
    <dbReference type="GeneID" id="1454480"/>
    <dbReference type="KEGG" id="sso:SSO1474"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1513"/>
    <dbReference type="eggNOG" id="arCOG02759">
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    <dbReference type="HOGENOM" id="CLU_101320_2_0_2"/>
    <dbReference type="InParanoid" id="Q97Y68"/>
    <dbReference type="PhylomeDB" id="Q97Y68"/>
    <dbReference type="BRENDA" id="2.7.7.B22">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97Y68"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1290">
        <property type="entry name" value="Transposase IS200-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002686">
        <property type="entry name" value="Transposase_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036515">
        <property type="entry name" value="Transposase_17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033573">
        <property type="entry name" value="transpos_IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33360">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33360:SF2">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01797">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01321">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143422">
        <property type="entry name" value="Transposase IS200-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4 5" id="KW-0002">3D-structure</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS200-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2F4F"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="2F5G"/>
        </source>
    </evidence>
    <sequence length="133" mass="15692" checksum="F5556B5DAB7F3AC3" modified="2001-10-01" version="1">MELKSTRHTKYLCNYHFVWIPKHRRNTLVNEIAEYTKEVLKSIAEELGCEIIALEVMPDHIHLFVNCPPRYAPSYLANYFKGKSARLILKKFPQLNKGKLWTRSYFVATAGNVSSEVIKKYIEEQWRKEGEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YA0</accession>
    <name>Q97YA0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR type III-associated protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1432</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2023" name="Curr. Res. Struct. Biol." volume="5" first="100098" last="100098">
            <title>Structure of the &lt;i&gt;Saccharolobus solfataricus&lt;/i&gt; type III-D CRISPR effector.</title>
            <authorList>
                <person name="Cannone G."/>
                <person name="Kompaniiets D."/>
                <person name="Graham S."/>
                <person name="White M.F."/>
                <person name="Spagnolo L."/>
            </authorList>
            <dbReference type="PubMed" id="36843655"/>
            <dbReference type="DOI" id="10.1016/j.crstbi.2023.100098"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (3.50 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90301">
        <property type="entry name" value="C90301"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923398.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="8BMW">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="N=1-252"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YA0"/>
    <dbReference type="SMR" id="Q97YA0"/>
    <dbReference type="STRING" id="273057.SSO1432"/>
    <dbReference type="PaxDb" id="273057-SSO1432"/>
    <dbReference type="EnsemblBacteria" id="AAK41666">
        <property type="protein sequence ID" value="AAK41666"/>
        <property type="gene ID" value="SSO1432"/>
    </dbReference>
    <dbReference type="GeneID" id="1454445"/>
    <dbReference type="KEGG" id="sso:SSO1432"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1459"/>
    <dbReference type="eggNOG" id="arCOG02658">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1197672_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YA0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="CRISPR_Csm3_endoribonuclease"/>
    </dbReference>
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        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35579">
        <property type="entry name" value="CRISPR SYSTEM CMS ENDORIBONUCLEASE CSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35579:SF6">
        <property type="entry name" value="DUF324 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="5" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="2">
        <location>
            <begin position="10"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="8BMW"/>
        </source>
    </evidence>
    <sequence length="252" mass="28917" checksum="1E28E53EAF36CEB5" modified="2001-10-01" version="1">MVIQVFRLNFRLRTGFRVGGGQEVGDNVIRQLRIGVEGVMIPASSWKGMFRRVSEIVLNSEDHFEEHSKKEVDTRTINALLKSDEKFRRIAISKVGKEVITQNGINVDKLDSESLSDLRRIYNEYNCPIERLYGSNYFAGGITISDSVIPNASIMERTHVTIERKSKKASEKHLFSEEIIDAEKIEVKVIVRNEFELWKNSLKLLREIGYFIGGSKSRGIGYIVLDEKESEYAVINNFSETPKFSELKRYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YB3</accession>
    <name>Q97YB3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90299">
        <property type="entry name" value="E90299"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988833.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YB3"/>
    <dbReference type="STRING" id="273057.SSO1418"/>
    <dbReference type="PaxDb" id="273057-SSO1418"/>
    <dbReference type="EnsemblBacteria" id="AAK41652">
        <property type="protein sequence ID" value="AAK41652"/>
        <property type="gene ID" value="SSO1418"/>
    </dbReference>
    <dbReference type="GeneID" id="1454431"/>
    <dbReference type="KEGG" id="sso:SSO1418"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1443"/>
    <dbReference type="eggNOG" id="arCOG03167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041527_2_0_2"/>
    <dbReference type="InParanoid" id="Q97YB3"/>
    <dbReference type="PhylomeDB" id="Q97YB3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025420">
        <property type="entry name" value="DUF4143"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295:SF18">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13635">
        <property type="entry name" value="DUF4143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="41"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="409" mass="47189" checksum="7283973E34820646" modified="2001-10-01" version="1">MEENIWWRGKDYIEEDEDIKKWNSAKFKWIPKEINKISLQPFSLNFIVGPRQVGKTTLIKLLIKKLLESNHNPLSIFYCSCDLVSDYEELLKRTRDYLKIRKQEGIKSSFIFLDEVTFVKEWYRAVKHLIDTGELTSDVVTVSGSSSLTILKQKESFPGRRGNGTDVILYPLSFSGYINVINPKIEVRPGWYSEIEELFESYLKVGGLPPSINGLSPLKTYTEWIIYEINRVGRDEMLAKQILSAVLSTTPSKVSYNSIAKEIGVNHRTVAEYIKLFNDMFLTLTLHFIDVNTGYYNYRKQIKIHFVDPLFYDVMSTWTGVKRPDDPIIVEGIVASHLSRIYSIGYTEVGKEEIDVVTLPEVIGYEVKYRERTKQVKVIAGKMKKVITLSKHGENDTFPVHLFLAELNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YB6</accession>
    <name>Q97YB6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1413</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90298">
        <property type="entry name" value="H90298"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923384.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YB6"/>
    <dbReference type="STRING" id="273057.SSO1413"/>
    <dbReference type="PaxDb" id="273057-SSO1413"/>
    <dbReference type="EnsemblBacteria" id="AAK41647">
        <property type="protein sequence ID" value="AAK41647"/>
        <property type="gene ID" value="SSO1413"/>
    </dbReference>
    <dbReference type="GeneID" id="1454426"/>
    <dbReference type="KEGG" id="sso:SSO1413"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1437"/>
    <dbReference type="eggNOG" id="arCOG07298">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1840677_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YB6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="139" mass="16072" checksum="07FDEDE60FCDECAE" modified="2001-10-01" version="1">MMSTSGFYQRSRRAIIYETDSLFRYTTEAELKFVIEKGVIMSYNPKGTYLTNLFTDDPNVAVKMLALSKLPRYRIGPISMSKKLFSKVKYVGIVTPKGKSRGGAKEYVFTDPIIIDVENLYVYDFKDRRTYTIRLPSIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YE2</accession>
    <name>Q97YE2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1078</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1384</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90295">
        <property type="entry name" value="C90295"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YE2"/>
    <dbReference type="STRING" id="273057.SSO1384"/>
    <dbReference type="PaxDb" id="273057-SSO1384"/>
    <dbReference type="EnsemblBacteria" id="AAK41618">
        <property type="protein sequence ID" value="AAK41618"/>
        <property type="gene ID" value="SSO1384"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1384"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1390"/>
    <dbReference type="eggNOG" id="arCOG02127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1718298_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YE2"/>
    <dbReference type="PhylomeDB" id="Q97YE2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051839">
        <property type="entry name" value="RD_transcriptional_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025959">
        <property type="entry name" value="Winged_HTH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33215">
        <property type="entry name" value="PROTEIN DISTAL ANTENNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33215:SF13">
        <property type="entry name" value="PROTEIN DISTAL ANTENNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13592">
        <property type="entry name" value="HTH_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Winged helix-turn helix" evidence="1">
        <location>
            <begin position="89"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="152" mass="18048" checksum="031C74C350FE62C4" modified="2001-10-01" version="1">MPKSRMHGIRLDKKQEEERRINAVKDVRNGMSIKDVAKKYDVSIFTVYKWLRKGDLSAKPRKGPTKLKDEEKLVRILEKSPRDFGLNYDFWTLKLIAYILDKEFGIKYNPRSLSPVLKKLGFKYKKGKRTYVRDDNAVERWVKEQGEKLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YL0</accession>
    <name>Q97YL0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ring oxydation complex/ phenylacetic acid degradation related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1313</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2013-09" db="PDB data bank">
            <title>Crystal structure of a ring oxydation complex/ phenylacetic acid degradation-like protein (SSO1313) from Sulfolobus solfataricus P2 at 2.43 A resolution.</title>
            <authorList>
                <consortium name="Joint Center for Structural Genomics (JCSG)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.43 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90286">
        <property type="entry name" value="F90286"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923306.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="4MUD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.43 A"/>
        <property type="chains" value="A/B/C/D=1-234"/>
    </dbReference>
    <dbReference type="PDBsum" id="4MUD"/>
    <dbReference type="AlphaFoldDB" id="Q97YL0"/>
    <dbReference type="SMR" id="Q97YL0"/>
    <dbReference type="STRING" id="273057.SSO1313"/>
    <dbReference type="PaxDb" id="273057-SSO1313"/>
    <dbReference type="DNASU" id="1454328"/>
    <dbReference type="EnsemblBacteria" id="AAK41549">
        <property type="protein sequence ID" value="AAK41549"/>
        <property type="gene ID" value="SSO1313"/>
    </dbReference>
    <dbReference type="GeneID" id="1454328"/>
    <dbReference type="KEGG" id="sso:SSO1313"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1314"/>
    <dbReference type="eggNOG" id="arCOG04786">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093768_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YL0"/>
    <dbReference type="PhylomeDB" id="Q97YL0"/>
    <dbReference type="EvolutionaryTrace" id="Q97YL0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052703">
        <property type="entry name" value="Aromatic_CoA_ox/epox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007814">
        <property type="entry name" value="PaaA_PaaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30458:SF0">
        <property type="entry name" value="1,2-PHENYLACETYL-COA EPOXIDASE, SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30458">
        <property type="entry name" value="PHENYLACETIC ACID DEGRADATION PROTEIN PAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05138">
        <property type="entry name" value="PaaA_PaaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="3" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="4MUD"/>
        </source>
    </evidence>
    <sequence length="234" mass="27280" checksum="2FD699786CD9897A" modified="2001-10-01" version="1">MQKLRSVKEVPQDLTNTLVNIIELRADFELAMVEQYSPWLVNAPTVDSRLFVAKLVSDELNHGWQLVRLLEEFKVKDVIERISNARLGIHKLEVSNLPLFNWEDVIAFTFLVDGAGLYQLKILKDCSFEPLSTLASSMIKEEESHIFFSQNELRNYQNKNRMQGAINFWFPRAVEMLHMTWSLNETHLRDLNISDLTKNDLINGYIKTTNEELKKCGYNEVNYDKALHYNVVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YQ4</accession>
    <name>Q97YQ4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90281">
        <property type="entry name" value="D90281"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YQ4"/>
    <dbReference type="STRING" id="273057.SSO1270"/>
    <dbReference type="PaxDb" id="273057-SSO1270"/>
    <dbReference type="EnsemblBacteria" id="AAK41507">
        <property type="protein sequence ID" value="AAK41507"/>
        <property type="gene ID" value="SSO1270"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1270"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1266"/>
    <dbReference type="eggNOG" id="arCOG01444">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031100_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YQ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="464" mass="54066" checksum="C985CD9BF8CB2B5F" modified="2001-10-01" version="1">MLRMIGKVLELLNCKETEREINRRKFSICDDVNFILNLPIGYLKDVISPLENYLSKHMTNNFSRLTRVVPNDYEDTQLHFSKFFLNLYSSAISEIPRFNILTSLVILDEYRMMPDKALIAAADELARNGINAIFSTSTPSVFFEEEIVSRFRNRRVVSISIVNEYGQAVEGRDCVKKGEGYYQCRGSYEVIELIEDFDIRDLDYEIIKGEEVFKVKEPFTLFVNSWEKALRLYRKLREERNTEVCLLNSSDECKVTITPKIMPTRFNVVSEISSLPTIVARSGIINDKDKIYVWIDRDNYDTVTESTLNLLSNIKVCLKHPFGCNGKMGYANKTVLKPNIDERLIKRLSEISHLMDRREFEKVTEVFSDFVTVDLFTDKEKITTVPPEFVYENKKKILYYGNSCVYTYFTDGEKCSKIVYNWLEGGGVSWLKRNLARAYKNYVEEEGKKKVFLGLKARDVEEWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YT4</accession>
    <name>Q97YT4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Catechol 2,3-dioxygenase (Metapyrocatechase)</fullName>
            <ecNumber evidence="3">1.13.11.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1223</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.13.11.2" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90276">
        <property type="entry name" value="G90276"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923261.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YT4"/>
    <dbReference type="FunCoup" id="Q97YT4">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1223"/>
    <dbReference type="PaxDb" id="273057-SSO1223"/>
    <dbReference type="DNASU" id="1454249"/>
    <dbReference type="EnsemblBacteria" id="AAK41470">
        <property type="protein sequence ID" value="AAK41470"/>
        <property type="gene ID" value="SSO1223"/>
    </dbReference>
    <dbReference type="GeneID" id="1454249"/>
    <dbReference type="KEGG" id="sso:SSO1223"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1224"/>
    <dbReference type="eggNOG" id="arCOG06037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052361_3_0_2"/>
    <dbReference type="InParanoid" id="Q97YT4"/>
    <dbReference type="PhylomeDB" id="Q97YT4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018577">
        <property type="term" value="F:catechol 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017624">
        <property type="entry name" value="Catechol_2-3_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050383">
        <property type="entry name" value="GlyoxalaseI/FosfomycinResist"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03211">
        <property type="entry name" value="catechol_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21366">
        <property type="entry name" value="GLYOXALASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21366:SF19">
        <property type="entry name" value="METAPYROCATECHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="VOC" evidence="2">
        <location>
            <begin position="8"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="2">
        <location>
            <begin position="155"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="335" mass="38026" checksum="44249E85668D4849" modified="2001-10-01" version="1">MVNKLLSQLASVEILTPKIDDTLWFFKDILGLHEVGRKDRSVYLRGWEDWYAYSLKVTESDTAGVKEIVWRTYSEKDLIDSINIVNSNGLGLGWDEGEPDMGVGKGYKFILPSGQIGKLVWEMKIWRASGELRSGYKARPMKKPTKGVQAKDLSHIFIFAYNDESLAKTVELLKRLTFKVNEILKEGDKRIAYWMAVNGTAHDLAVSLDPSGVPARLNHVTFNVDYADDILKAADFYTDYGLEIVGGPLRHGITDSHSLYVKEPGGNVIELLHGGYLNVVPDWEPIVWDTKEDENRWLYYWGHVKEAFWHGSPIPANRVSDELKEIVRQKLGAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YU9</accession>
    <name>Q97YU9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="4">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">porA-1</name>
        <name evidence="9" type="ordered locus">SSO1207</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90274">
        <property type="entry name" value="G90274"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990065.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YU9"/>
    <dbReference type="FunCoup" id="Q97YU9">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1207"/>
    <dbReference type="PaxDb" id="273057-SSO1207"/>
    <dbReference type="EnsemblBacteria" id="AAK41454">
        <property type="protein sequence ID" value="AAK41454"/>
        <property type="gene ID" value="SSO1207"/>
    </dbReference>
    <dbReference type="GeneID" id="1454233"/>
    <dbReference type="KEGG" id="sso:SSO1207"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1205"/>
    <dbReference type="eggNOG" id="arCOG01608">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002569_5_0_2"/>
    <dbReference type="InParanoid" id="Q97YU9"/>
    <dbReference type="PhylomeDB" id="Q97YU9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000010">
        <property type="entry name" value="Pyruvate ferredoxin oxidoreductase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000012">
        <property type="entry name" value="Pyruvate:ferredoxin (Flavodoxin) oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF0">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="7">
        <location>
            <begin position="20"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate:ferredoxin oxidoreductase core" evidence="8">
        <location>
            <begin position="265"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="385" mass="43604" checksum="371DB7727D96F5A0" modified="2001-10-01" version="1">MQVLKRKVLALVGNHAVAYAVKQAKPKVLAVFPITPQTTMLEKLSEYISSEELKAELIKVESEHSALASIYGAALAGARVFTATSSQGLLYMTEMIYWAGGQRVPIVAAVATRAIAEPWSIWDDHQDFVSKRDAIWIQIMAENVQEAYDMTIQAFRISEDERVILPVMMGFDGFILTHTMERIEVLEDNEVDNFLPPRQFNLIDFSDPIAIGPIATPEEYIKYRYEAMKAMERAKGVIEEIMGEYERISGRKQHGLVECYKCEDAKYVFVTMGAWSGDGKAAVDRLRDSGVKTGLLKIRVFRPFPKEKVEEYLRSMKGVVVFDRAYSYGYGGILVNEIKAALYGYRVPVYSVVAGIGGKDVRPRHFQKVIEDLINDNLEEERWLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YY1</accession>
    <name>Q97YY1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Maltose ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1171</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90270">
        <property type="entry name" value="D90270"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923220.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YY1"/>
    <dbReference type="FunCoup" id="Q97YY1">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1171"/>
    <dbReference type="PaxDb" id="273057-SSO1171"/>
    <dbReference type="EnsemblBacteria" id="AAK41419">
        <property type="protein sequence ID" value="AAK41419"/>
        <property type="gene ID" value="SSO1171"/>
    </dbReference>
    <dbReference type="GeneID" id="1454198"/>
    <dbReference type="KEGG" id="sso:SSO1171"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1164"/>
    <dbReference type="eggNOG" id="arCOG00154">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_568182_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YY1"/>
    <dbReference type="PhylomeDB" id="Q97YY1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055052">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901982">
        <property type="term" value="F:maltose binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042956">
        <property type="term" value="P:maltodextrin transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015768">
        <property type="term" value="P:maltose transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13522">
        <property type="entry name" value="PBP2_ABC_oligosaccharides"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30061">
        <property type="entry name" value="MALTOSE-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30061:SF50">
        <property type="entry name" value="MALTOSE_MALTODEXTRIN-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="523" mass="56856" checksum="6E4BA186840859D8" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z07</accession>
    <name>Q97Z07_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90266">
        <property type="entry name" value="H90266"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989705.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z07"/>
    <dbReference type="STRING" id="273057.SSO1140"/>
    <dbReference type="PaxDb" id="273057-SSO1140"/>
    <dbReference type="EnsemblBacteria" id="AAK41391">
        <property type="protein sequence ID" value="AAK41391"/>
        <property type="gene ID" value="SSO1140"/>
    </dbReference>
    <dbReference type="GeneID" id="1454170"/>
    <dbReference type="KEGG" id="sso:SSO1140"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1131"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_697573_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z07"/>
    <dbReference type="PhylomeDB" id="Q97Z07"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17370">
        <property type="entry name" value="MFS_MJ1317_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518">
        <property type="entry name" value="C-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="163"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="383" mass="42512" checksum="F4269BC5D108058D" modified="2001-10-01" version="1">MLKNRNEVLKISFSAFFADLGYQAVVASFPIIFVLIFKAPIPLYGFAEALNYGIGTVMAYAGGLAGDRFGRKRIAILGNVLILFTSLIGLSRDYIQALIFFMIGWWFRNFRSPPRRAMMAEVTSPEERSEAFGILHSLDIAGALIAIIYLTVLLYLRVSIFFVLLFTSIPLLMSTIVLTMVNAGKKSEKAKRKEAESKITQKRVFWTLILSTMFFGFSQYSFGFPILTTTEITGKEYLGVLSYGIFLGASSLFGYLFGRIRMKEFESLAFLGYLIGALGSLGFAYLSSFGVFSLYPLSFLMGTSVASTETFEPTIISKITKEEAFSTSMGYLSAGRSIGIFLGNVIMGFLYQISYTYAYLFAAITSLISFALILNLIMRPNAS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z80</accession>
    <name>RS27_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein eS27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 5" type="primary">rps27e</name>
        <name type="ordered locus">SSO7114</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6 7 8 9 10 11 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1 6 7 8 9 10 11 12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90257">
        <property type="entry name" value="B90257"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="W=1-66"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="W=1-66"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="W=1-66"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="W=1-66"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="W=1-66"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="W=1-66"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="W=1-66"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="W=1-66"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q97Z80"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q97Z80"/>
    <dbReference type="FunCoup" id="Q97Z80">
        <property type="interactions" value="207"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO7114"/>
    <dbReference type="PaxDb" id="273057-SSO7114"/>
    <dbReference type="EnsemblBacteria" id="AAK41313">
        <property type="protein sequence ID" value="AAK41313"/>
        <property type="gene ID" value="SSO7114"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7114"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1045"/>
    <dbReference type="eggNOG" id="arCOG04108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199465_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z80"/>
    <dbReference type="PhylomeDB" id="Q97Z80"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00371">
        <property type="entry name" value="Ribosomal_eS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_eS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_eS27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001629">
        <property type="entry name" value="PRK00415.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11594">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01168">
        <property type="entry name" value="RIBOSOMAL_S27E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000149082" description="Small ribosomal subunit protein eS27">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 6 7">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 6 7">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 6 7">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 6 7">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FRL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="10">
        <source>
            <dbReference type="PDB" id="9FS6"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="11">
        <source>
            <dbReference type="PDB" id="9FS8"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="12">
        <source>
            <dbReference type="PDB" id="9FY0"/>
        </source>
    </evidence>
    <sequence length="66" mass="7385" checksum="DC33AF61C4A6CF9A" modified="2001-10-01" version="1">MMRKLRVLIPEPKSRFLRVKCPNCGNEQTIFSHATFPVRCLSCGTELVYSMGGKAKIVGEVVRIMG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-01-31" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZF1</accession>
    <name>DHYS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Probable deoxyhypusine synthase</fullName>
            <shortName>DHS</shortName>
            <ecNumber>2.5.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">dys</name>
        <name type="ordered locus">SSO0967</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-deoxyhypusine + propane-1,3-diamine</text>
            <dbReference type="Rhea" id="RHEA:33299"/>
            <dbReference type="Rhea" id="RHEA-COMP:10143"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57484"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="EC" id="2.5.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.46"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90248">
        <property type="entry name" value="B90248"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992414.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZF1"/>
    <dbReference type="SMR" id="Q97ZF1"/>
    <dbReference type="FunCoup" id="Q97ZF1">
        <property type="interactions" value="217"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0967"/>
    <dbReference type="PaxDb" id="273057-SSO0967"/>
    <dbReference type="EnsemblBacteria" id="AAK41241">
        <property type="protein sequence ID" value="AAK41241"/>
        <property type="gene ID" value="SSO0967"/>
    </dbReference>
    <dbReference type="GeneID" id="1455208"/>
    <dbReference type="KEGG" id="sso:SSO0967"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.966"/>
    <dbReference type="eggNOG" id="arCOG04142">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039781_1_0_2"/>
    <dbReference type="InParanoid" id="Q97ZF1"/>
    <dbReference type="PhylomeDB" id="Q97ZF1"/>
    <dbReference type="BRENDA" id="2.5.1.46">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00354"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008216">
        <property type="term" value="P:spermidine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.910.10:FF:000007">
        <property type="entry name" value="Probable deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00153">
        <property type="entry name" value="DHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022899">
        <property type="entry name" value="Deoxyhypus_synthase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002294">
        <property type="entry name" value="PRK01221.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF0">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000134508" description="Probable deoxyhypusine synthase">
        <location>
            <begin position="1"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="312" mass="35050" checksum="A9072CE18825A2D9" modified="2001-10-01" version="1">MINREDLLKNPVEDITLSDLKKYNDIVSVFDKIYGFSSEGIVNGSKILKEMIKNADLRFLSFTANLVSTGLRGLFADLIKKGYFNIVVTTGGTIDHDLARSFGGVYYKGSFDIDDTMLKDLEIHRLGNVLVPFESYGKVIEDVVRKFLPEITKDRKEISAYELLWEFGKRITDSNSILRAAYDKNVPIIVPGILDGSFGTNLFIQSQFLNFRINLFEDMRLIKDLIFSSKKSGALIIGGGISKHHTIWWNQFKDGLNYAIYITTAQEYDGSLSGAKPREAISWNKIRPDAKHVTIYGDATIIVPILAASLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="139" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZF9</accession>
    <name>Q97ZF9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA repair protein rad25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO0959</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90247">
        <property type="entry name" value="B90247"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992401.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZF9"/>
    <dbReference type="FunCoup" id="Q97ZF9">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0959"/>
    <dbReference type="PaxDb" id="273057-SSO0959"/>
    <dbReference type="EnsemblBacteria" id="AAK41233">
        <property type="protein sequence ID" value="AAK41233"/>
        <property type="gene ID" value="SSO0959"/>
    </dbReference>
    <dbReference type="GeneID" id="1455200"/>
    <dbReference type="KEGG" id="sso:SSO0959"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.956"/>
    <dbReference type="eggNOG" id="arCOG00874">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008213_6_0_2"/>
    <dbReference type="InParanoid" id="Q97ZF9"/>
    <dbReference type="PhylomeDB" id="Q97ZF9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17926">
        <property type="entry name" value="DEXHc_RE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050615">
        <property type="entry name" value="ATP-dep_DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274">
        <property type="entry name" value="RAD25/XP-B DNA REPAIR HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="173"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="406"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="551" mass="64175" checksum="FB8F968A0CD97E63" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZG9</accession>
    <name>Q97ZG9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0944</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90245">
        <property type="entry name" value="E90245"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992379.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZG9"/>
    <dbReference type="SMR" id="Q97ZG9"/>
    <dbReference type="STRING" id="273057.SSO0944"/>
    <dbReference type="PaxDb" id="273057-SSO0944"/>
    <dbReference type="EnsemblBacteria" id="AAK41220">
        <property type="protein sequence ID" value="AAK41220"/>
        <property type="gene ID" value="SSO0944"/>
    </dbReference>
    <dbReference type="GeneID" id="1455187"/>
    <dbReference type="KEGG" id="sso:SSO0944"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.940"/>
    <dbReference type="eggNOG" id="arCOG04153">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142666_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZG9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007832">
        <property type="entry name" value="RNA_pol_Rpc34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016049">
        <property type="entry name" value="RNA_pol_Rpc34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12780">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED , 39KD SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05158">
        <property type="entry name" value="RNA_pol_Rpc34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="125" mass="13872" checksum="BB85BD6B7FE221BD" modified="2001-10-01" version="1">MESDSTSNYEAIVHKKIKEMGEKGISQQELARSVGLPIREISSIIKRLVEKKLIIKKAVKENGKNIVKLFAVDNNYNINIYINLNGLDEIPCLSCKSLTKCGNGIHVSPQTCSKLSGWIIEKALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZH2</accession>
    <name>Q97ZH2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF61 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0941</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2018" name="J. Struct. Biol." volume="203" first="179" last="184">
            <title>Solution structure of an archaeal DUF61 family protein SSO0941 encoded by a gene in the operon of box C/D RNA protein complexes.</title>
            <authorList>
                <person name="Zhou T."/>
                <person name="Yao X."/>
                <person name="Wang J."/>
                <person name="Feng Y."/>
            </authorList>
            <dbReference type="PubMed" id="29526782"/>
            <dbReference type="DOI" id="10.1016/j.jsb.2018.03.003"/>
        </citation>
        <scope>STRUCTURE BY NMR</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90245">
        <property type="entry name" value="B90245"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992374.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="5VFK">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-138"/>
    </dbReference>
    <dbReference type="PDBsum" id="5VFK"/>
    <dbReference type="AlphaFoldDB" id="Q97ZH2"/>
    <dbReference type="SMR" id="Q97ZH2"/>
    <dbReference type="STRING" id="273057.SSO0941"/>
    <dbReference type="PaxDb" id="273057-SSO0941"/>
    <dbReference type="EnsemblBacteria" id="AAK41217">
        <property type="protein sequence ID" value="AAK41217"/>
        <property type="gene ID" value="SSO0941"/>
    </dbReference>
    <dbReference type="GeneID" id="1455184"/>
    <dbReference type="KEGG" id="sso:SSO0941"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.937"/>
    <dbReference type="eggNOG" id="arCOG01921">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850723_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZH2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012357">
        <property type="entry name" value="UCP024484"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002746">
        <property type="entry name" value="UPF0216"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01886">
        <property type="entry name" value="DUF61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF024484">
        <property type="entry name" value="UCP024484"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="3" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="5VFK"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="16040" checksum="422D754AAEE99CB2" modified="2001-10-01" version="1">MIDKIFEIGLKDIFSSSPAEYVTIKDALDGKLKIRLNNNFYHEIKKDEVEKLSSRIPLYLWSLVKIPFIFIKSSEIGEYFVSGEQWNKKAISILLGREISNVILNVDVEKLLREYTSLIFIILSPTRSYTEETELSEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZK8</accession>
    <name>Q97ZK8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD(P)/FAD-dependent oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0898</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90240">
        <property type="entry name" value="E90240"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992321.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZK8"/>
    <dbReference type="STRING" id="273057.SSO0898"/>
    <dbReference type="PaxDb" id="273057-SSO0898"/>
    <dbReference type="EnsemblBacteria" id="AAK41180">
        <property type="protein sequence ID" value="AAK41180"/>
        <property type="gene ID" value="SSO0898"/>
    </dbReference>
    <dbReference type="GeneID" id="1455147"/>
    <dbReference type="KEGG" id="sso:SSO0898"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.901"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066799_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZK8"/>
    <dbReference type="PhylomeDB" id="Q97ZK8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000579">
        <property type="entry name" value="FAD dependent oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF20">
        <property type="entry name" value="HYDROGENASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="38923" checksum="A552DE6859CE81D2" modified="2001-10-01" version="1">MRIAILGGGVAGSTLAYLLSRINYEVTIFDINQHYVKPCGDIVPNIYTPPFNWKETFGIRRFSFYIDGEKIYDVEYRHTKWLVIDKWKWINDMRKSSKVIVTHDNNAKEFDYVIDSRGPYPMDREVVYTTRAIIETEEFDDEAILEFNTRYTGFYWIFPSKENEYNIGAGFLEYKNSKELLNYYLRKRFKNFEVKDIRGAPISIGSVKNKKLRIGEARGLVFPLSGEGIRPSAISAEYAFEAIHKEKDLDGFLNEKLSIIEKRIKIQKMLLDLYRYSSLNLRKTFMRIFFKNEVLIDTYLEDKMDLNGIIESLKSVRANGGIIRKFEEQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZV0</accession>
    <name>Q97ZV0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90192">
        <property type="entry name" value="G90192"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922992.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZV0"/>
    <dbReference type="STRING" id="273057.SSO0477"/>
    <dbReference type="PaxDb" id="273057-SSO0477"/>
    <dbReference type="EnsemblBacteria" id="AAK40798">
        <property type="protein sequence ID" value="AAK40798"/>
        <property type="gene ID" value="SSO0477"/>
    </dbReference>
    <dbReference type="GeneID" id="1455608"/>
    <dbReference type="KEGG" id="sso:SSO0477"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.471"/>
    <dbReference type="eggNOG" id="arCOG01357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032214_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZV0"/>
    <dbReference type="PhylomeDB" id="Q97ZV0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731:SF4">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731">
        <property type="entry name" value="SLL1084 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="220"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="541" mass="61550" checksum="059C50BC606D2BE8" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZZ2</accession>
    <name>Q97ZZ2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0429</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90187">
        <property type="entry name" value="C90187"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922969.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZZ2"/>
    <dbReference type="STRING" id="273057.SSO0429"/>
    <dbReference type="PaxDb" id="273057-SSO0429"/>
    <dbReference type="EnsemblBacteria" id="AAK40754">
        <property type="protein sequence ID" value="AAK40754"/>
        <property type="gene ID" value="SSO0429"/>
    </dbReference>
    <dbReference type="GeneID" id="1455564"/>
    <dbReference type="KEGG" id="sso:SSO0429"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.421"/>
    <dbReference type="eggNOG" id="arCOG02459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_859465_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZZ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.50">
        <property type="entry name" value="ATP synthase (C/AC39) subunit, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036079">
        <property type="entry name" value="ATPase_csu/dsu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044911">
        <property type="entry name" value="V-type_ATPase_csu/dsu_dom_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="V-type ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="37203" checksum="62E13DE5DA57AC41" modified="2001-10-01" version="1">MSYAIYSFIHSISRTQKVSLLTKGLVNELISSESWNNVASLLKERGIIEEQPASLEDFEYMLKSRSLTLLEKIRNYFSIFRVTYNIVDLYIYMISLDELKNIIVSIVNGTGNGNSNKIRFFRKYFDQIPSSLEELMNSFKGNVYANALSYAIKDGQGKNISYLLSLLDIYFIKKLSEIIEGFKGDWKSLAENIICYYKDYYSISLAIKHKTVENTVCKIGTEILKDLSSSTSDAETLDILRRTQYSKLLNVNSTYGALASMYRIARINARKNSELVFMSSPFNPALALALAELIRLDTEDIISIANAKSLRLKEEKIKNMLSFEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q980A2</accession>
    <name>Q980A2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription elongation factor Spt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpoE2</name>
        <name evidence="2" type="synonym">spt4</name>
        <name evidence="4" type="ordered locus">SSO5798</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of Spt4 and Spt5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal Spt4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90185">
        <property type="entry name" value="H90185"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988766.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980A2"/>
    <dbReference type="STRING" id="273057.SSO5798"/>
    <dbReference type="PaxDb" id="273057-SSO5798"/>
    <dbReference type="EnsemblBacteria" id="AAK40743">
        <property type="protein sequence ID" value="AAK40743"/>
        <property type="gene ID" value="SSO5798"/>
    </dbReference>
    <dbReference type="GeneID" id="1455553"/>
    <dbReference type="GeneID" id="44129395"/>
    <dbReference type="KEGG" id="sso:SSO5798"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.410"/>
    <dbReference type="eggNOG" id="arCOG04077">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199467_0_0_2"/>
    <dbReference type="InParanoid" id="Q980A2"/>
    <dbReference type="PhylomeDB" id="Q980A2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00949">
        <property type="entry name" value="Spt4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007178">
        <property type="entry name" value="Spt4_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038589">
        <property type="entry name" value="Spt4_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041664">
        <property type="entry name" value="RNAP_arch_Epp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40704">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40704:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Spt4/RpoE2 zinc finger" evidence="3">
        <location>
            <begin position="7"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="66" mass="7341" checksum="CF7F0B536B99A8C1" modified="2001-10-01" version="1">MAKKSVFKACKNCKALVDTDKETCPVCGSTSFTDEWDGMIIIINSESEIAKITEAPKPWKYAIIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q980F3</accession>
    <name>Q980F3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0359</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90179">
        <property type="entry name" value="B90179"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990655.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980F3"/>
    <dbReference type="STRING" id="273057.SSO0359"/>
    <dbReference type="PaxDb" id="273057-SSO0359"/>
    <dbReference type="EnsemblBacteria" id="AAK40689">
        <property type="protein sequence ID" value="AAK40689"/>
        <property type="gene ID" value="SSO0359"/>
    </dbReference>
    <dbReference type="GeneID" id="1455499"/>
    <dbReference type="KEGG" id="sso:SSO0359"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.350"/>
    <dbReference type="eggNOG" id="arCOG02067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121282_0_0_2"/>
    <dbReference type="InParanoid" id="Q980F3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655:SF3">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655">
        <property type="entry name" value="CONSERVED WITHIN P. AEROPHILUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DsrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="133" mass="14779" checksum="A22DB75FAC37217F" modified="2001-10-01" version="1">MSKITILLADNNIDKLYHGLVVALGAKALGWDVKFFVTSQAVVLFTKAKKGKGKLGLPFFARFFVRLQMKRLNIPNPEKLIDDAINQGVEFFVDEAGLRLVNARSEDLLENVKLSGTITFILEAKESDVVITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q980I2</accession>
    <name>Q980I2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="15">DNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">SSO0313</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90174">
        <property type="entry name" value="B90174"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990605.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980I2"/>
    <dbReference type="FunCoup" id="Q980I2">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0313"/>
    <dbReference type="PaxDb" id="273057-SSO0313"/>
    <dbReference type="EnsemblBacteria" id="AAK40649">
        <property type="protein sequence ID" value="AAK40649"/>
        <property type="gene ID" value="SSO0313"/>
    </dbReference>
    <dbReference type="GeneID" id="1455459"/>
    <dbReference type="KEGG" id="sso:SSO0313"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.306"/>
    <dbReference type="eggNOG" id="arCOG00770">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006515_9_0_2"/>
    <dbReference type="InParanoid" id="Q980I2"/>
    <dbReference type="PhylomeDB" id="Q980I2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001813">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002255">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF34">
        <property type="entry name" value="REGULATOR OF TELOMERE ELONGATION HELICASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="15"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAK40649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="550" mass="63447" checksum="30ED83C55DFE2405" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q980L1</accession>
    <name>Q980L1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TFIIB-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90170">
        <property type="entry name" value="D90170"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990562.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980L1"/>
    <dbReference type="STRING" id="273057.SSO0280"/>
    <dbReference type="PaxDb" id="273057-SSO0280"/>
    <dbReference type="EnsemblBacteria" id="AAK40619">
        <property type="protein sequence ID" value="AAK40619"/>
        <property type="gene ID" value="SSO0280"/>
    </dbReference>
    <dbReference type="GeneID" id="1455429"/>
    <dbReference type="KEGG" id="sso:SSO0280"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.275"/>
    <dbReference type="eggNOG" id="arCOG05931">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1393599_0_0_2"/>
    <dbReference type="InParanoid" id="Q980L1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="170" mass="19968" checksum="2E33F3A92ADF6AAB" modified="2001-10-01" version="1">MQCPACGSNEIIWDNKNGEIVCSNCGTIIDNIYYNGQNESESTEIISINNKFYKDDIQVKELRVKNFLKNNRIETKKIDQYEIILRSMLLDSQYKKIYKLLYDEGILSGLKAKSKLGLLIYFRFALNNGYLHLLEKFDIKNETLKKILKRIGRKRLTLLFDKLNEESDRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-05" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Q8</accession>
    <name>IF2P_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable translation initiation factor IF-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">infB</name>
        <name type="ordered locus">SSO0228</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90164">
        <property type="entry name" value="C90164"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990481.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Q8"/>
    <dbReference type="SMR" id="Q980Q8"/>
    <dbReference type="FunCoup" id="Q980Q8">
        <property type="interactions" value="138"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0228"/>
    <dbReference type="PaxDb" id="273057-SSO0228"/>
    <dbReference type="EnsemblBacteria" id="AAK40570">
        <property type="protein sequence ID" value="AAK40570"/>
        <property type="gene ID" value="SSO0228"/>
    </dbReference>
    <dbReference type="GeneID" id="1455380"/>
    <dbReference type="GeneID" id="44129198"/>
    <dbReference type="KEGG" id="sso:SSO0228"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.225"/>
    <dbReference type="eggNOG" id="arCOG01560">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002656_3_3_2"/>
    <dbReference type="InParanoid" id="Q980Q8"/>
    <dbReference type="PhylomeDB" id="Q980Q8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16266">
        <property type="entry name" value="IF2_aeIF5B_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000013">
        <property type="entry name" value="eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000152">
        <property type="entry name" value="Probable translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_A">
        <property type="entry name" value="IF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004544">
        <property type="entry name" value="TF_aIF-2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00491">
        <property type="entry name" value="aIF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003078">
        <property type="entry name" value="PRK04004.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000137308" description="Probable translation initiation factor IF-2">
        <location>
            <begin position="1"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="10"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="44"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="83"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="137"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="205"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="83"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="137"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00100"/>
        </source>
    </evidence>
    <sequence length="600" mass="66934" checksum="74F21DF4F4C05287" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q980S4</accession>
    <name>Q980S4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1411 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0203</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90161">
        <property type="entry name" value="F90161"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990448.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980S4"/>
    <dbReference type="STRING" id="273057.SSO0203"/>
    <dbReference type="PaxDb" id="273057-SSO0203"/>
    <dbReference type="EnsemblBacteria" id="AAK40549">
        <property type="protein sequence ID" value="AAK40549"/>
        <property type="gene ID" value="SSO0203"/>
    </dbReference>
    <dbReference type="GeneID" id="1455359"/>
    <dbReference type="KEGG" id="sso:SSO0203"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.203"/>
    <dbReference type="eggNOG" id="arCOG04105">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140139_0_0_2"/>
    <dbReference type="InParanoid" id="Q980S4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="17922" checksum="4F561341D80D6286" modified="2001-10-01" version="1">MSQPGQQKSKDVKMVSKQVISTNIINEDKRKLQLLYIIRQIGKLTEKSIIMLLYELKQKGIDLGYQFNVIGNNVFSLMAKEDLTSLLYLGLVENDPVTKKISISNNGIEILDKTQIDDDFKNKLAQALNEIKTKILAYDEEYNLKIKNEMKLRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q980U3</accession>
    <name>Q980U3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Uroporphyrinogen-III synthase</fullName>
            <ecNumber evidence="3 7">4.2.1.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO0184</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>hydroxymethylbilane = uroporphyrinogen III + H2O</text>
            <dbReference type="Rhea" id="RHEA:18965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="EC" id="4.2.1.75"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the uroporphyrinogen-III synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.75" evidence="3 7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90159">
        <property type="entry name" value="C90159"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990420.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980U3"/>
    <dbReference type="FunCoup" id="Q980U3">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0184"/>
    <dbReference type="PaxDb" id="273057-SSO0184"/>
    <dbReference type="EnsemblBacteria" id="AAK40530">
        <property type="protein sequence ID" value="AAK40530"/>
        <property type="gene ID" value="SSO0184"/>
    </dbReference>
    <dbReference type="GeneID" id="1455340"/>
    <dbReference type="KEGG" id="sso:SSO0184"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.182"/>
    <dbReference type="eggNOG" id="arCOG02048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1253635_0_0_2"/>
    <dbReference type="InParanoid" id="Q980U3"/>
    <dbReference type="PhylomeDB" id="Q980U3"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006780">
        <property type="term" value="P:uroporphyrinogen III biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38042">
        <property type="entry name" value="UROPORPHYRINOGEN-III SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38042:SF1">
        <property type="entry name" value="UROPORPHYRINOGEN-III SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="HemD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 7" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrapyrrole biosynthesis uroporphyrinogen III synthase" evidence="8">
        <location>
            <begin position="22"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK40530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="237" mass="27328" checksum="AE1C85DAEE7427F6" modified="2001-10-01" version="1">MRVLFLRPDNEDNELNEKLMILRKNGIEVLNIPIFKIKCVSYSLPTYDYEALAFTSRNSVICFKDRTLIKNVHKIYAIGEETAELLMKMYNVNPITPERFTSIELAKRILEDKVNSILSIRSRKASEDMRNILNGKIKYDEIYVYDSEIIRDNINEISKILTECEVDAIAFTSSLMAKLIGPFIRGKCNIIIFSIGPMTTETLKRVNNKVKIIESKTHSIKGIIETILVEMKRNGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q980X5</accession>
    <name>Q980X5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90155">
        <property type="entry name" value="B90155"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980X5"/>
    <dbReference type="STRING" id="273057.SSO0150"/>
    <dbReference type="PaxDb" id="273057-SSO0150"/>
    <dbReference type="EnsemblBacteria" id="AAK40497">
        <property type="protein sequence ID" value="AAK40497"/>
        <property type="gene ID" value="SSO0150"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0150"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.147"/>
    <dbReference type="eggNOG" id="arCOG05934">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167325_0_0_2"/>
    <dbReference type="InParanoid" id="Q980X5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="118" mass="14090" checksum="8E8EB4D707E72670" modified="2001-10-01" version="1">MVVRKEWVLNIIYLIYITILSMSTRWLPKWKAIEIDYNNKKVTVCYDEVTRLYVCPICSPNCAKGVSTDYSTYFFNLEDLKRHLDAHKYGLWLQKKTRTEEEEEEPKLSIGEEESEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q981A2</accession>
    <name>Q981A2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0046</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <ligand>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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                <person name="Van der Oost J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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        <citation type="journal article" date="2024" name="Nucleic Acids Res." volume="52" first="9966" last="9977">
            <title>Unraveling the structure and function of a novel SegC protein interacting with the SegAB chromosome segregation complex in Archaea.</title>
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                <person name="Lin M.G."/>
                <person name="Yen C.Y."/>
                <person name="Shen Y.Y."/>
                <person name="Huang Y.S."/>
                <person name="Ng I.W."/>
                <person name="Barilla D."/>
                <person name="Sun Y.J."/>
                <person name="Hsiao C.D."/>
            </authorList>
            <dbReference type="PubMed" id="39077943"/>
            <dbReference type="DOI" id="10.1093/nar/gkae660"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH ADP AND MG(2+)</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
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</entry>
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    <accession>Q9UXC6</accession>
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    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
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                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
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            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
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                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90222">
        <property type="entry name" value="C90222"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991312.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXC6"/>
    <dbReference type="SMR" id="Q9UXC6"/>
    <dbReference type="FunCoup" id="Q9UXC6">
        <property type="interactions" value="205"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0738"/>
    <dbReference type="PaxDb" id="273057-SSO0738"/>
    <dbReference type="EnsemblBacteria" id="AAK41034">
        <property type="protein sequence ID" value="AAK41034"/>
        <property type="gene ID" value="SSO0738"/>
    </dbReference>
    <dbReference type="GeneID" id="1455001"/>
    <dbReference type="GeneID" id="44129734"/>
    <dbReference type="KEGG" id="sso:SSO0738"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.734"/>
    <dbReference type="eggNOG" id="arCOG00971">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_4_1_2"/>
    <dbReference type="InParanoid" id="Q9UXC6"/>
    <dbReference type="PhylomeDB" id="Q9UXC6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03756">
        <property type="entry name" value="proteasome_alpha_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000004">
        <property type="entry name" value="Proteasome subunit alpha type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00289_A">
        <property type="entry name" value="Proteasome_A_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019982">
        <property type="entry name" value="Proteasome_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03633">
        <property type="entry name" value="arc_protsome_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0647">Proteasome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000124187" description="Proteasome subunit alpha">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00289"/>
        </source>
    </evidence>
    <sequence length="241" mass="26589" checksum="9B73A9B506E72911" modified="2000-05-01" version="1">MAFGPAAMGYDRAITIFSPDGSLYQVDYAFEAVKKGWTAIGIKSKSSVVIASEKRKAQSLLDVDSIEKVFLIDDHVGCSFAGLASDGRVLIDYARNIALQHRLIYDEPVSIDYLTKSVADVKQMYTQHGGVRPFGVALVIAGIDKSVPKLFMTEPSGQYMPYQAVAIGQGYYTATEFLEKNYKEDLNVEETILLALKALSATLKPNEKLTPNTVEIGYASTQTGLFLKMTNEDKNMYLQKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-04-27" modified="2025-06-18" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXG8</accession>
    <name>Y781_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>UPF0148 protein SSO0781</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0781</name>
        <name type="ORF">C40_017</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0148 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90227">
        <property type="entry name" value="G90227"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923073.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXG8"/>
    <dbReference type="SMR" id="Q9UXG8"/>
    <dbReference type="PaxDb" id="273057-SSO0781"/>
    <dbReference type="EnsemblBacteria" id="AAK41078">
        <property type="protein sequence ID" value="AAK41078"/>
        <property type="gene ID" value="SSO0781"/>
    </dbReference>
    <dbReference type="GeneID" id="84061674"/>
    <dbReference type="KEGG" id="sso:SSO0781"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.782"/>
    <dbReference type="eggNOG" id="arCOG00578">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142653_1_0_2"/>
    <dbReference type="InParanoid" id="Q9UXG8"/>
    <dbReference type="PhylomeDB" id="Q9UXG8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00343">
        <property type="entry name" value="UPF0148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009563">
        <property type="entry name" value="SSSCA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022954">
        <property type="entry name" value="UPF0148"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001644">
        <property type="entry name" value="PRK00420.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001647">
        <property type="entry name" value="PRK00420.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06677">
        <property type="entry name" value="Auto_anti-p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000159860" description="UPF0148 protein SSO0781">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="118" mass="13445" checksum="C92FE8819473D8B2" modified="2000-05-01" version="1">MTNESEVGVKKAAELLRQGATMLEEACPICKMPLFKLKNGDVVCPVHGKVYIVKSDDEEKIVKRNLQLDEIESILIDGLYLSAKKMKEDPLDSERIIQIIRYLDALERLRKIKINSSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1995-02-01" modified="2025-06-18" version="147" xmlns="https://uniprot.org/uniprot">
    <accession>P39461</accession>
    <name>FUMC_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Fumarate hydratase class II</fullName>
            <shortName evidence="1 4">Fumarase C</shortName>
            <ecNumber evidence="1 2">4.2.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aerobic fumarase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Iron-independent fumarase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 4" type="primary">fumC</name>
        <name type="ordered locus">SSO1077</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1994" name="FEBS Lett." volume="337" first="93" last="98">
            <title>Molecular cloning, nucleotide sequence and expression of a Sulfolobus solfataricus gene encoding a class II fumarase.</title>
            <authorList>
                <person name="Colombo S."/>
                <person name="Grisa M."/>
                <person name="Tortora P."/>
                <person name="Vanoni M."/>
            </authorList>
            <dbReference type="PubMed" id="8276121"/>
            <dbReference type="DOI" id="10.1016/0014-5793(94)80636-5"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 1-14</scope>
        <scope>FUNCTION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1994" name="FEBS Lett." volume="340" first="151" last="153">
            <authorList>
                <person name="Colombo S."/>
                <person name="Grisa M."/>
                <person name="Tortora P."/>
                <person name="Vanoni M."/>
            </authorList>
            <dbReference type="PubMed" id="8119400"/>
            <dbReference type="DOI" id="10.1016/0014-5793(94)80192-4"/>
        </citation>
        <scope>ERRATUM OF PUBMED:8276121</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="1990" name="J. Gen. Microbiol." volume="136" first="1537" last="1541">
            <title>Purification and properties of a thermostable fumarate hydratase from the archaeobacterium Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Puchegger S."/>
                <person name="Redl B."/>
                <person name="Stoeffler G."/>
            </authorList>
            <dbReference type="PubMed" id="2124611"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35091 / DSM 1616 / IFO 15331 / JCM 8930 / P1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2 3">Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>(S)-malate = fumarate + H2O</text>
            <dbReference type="Rhea" id="RHEA:12460"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="EC" id="4.2.1.2"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">0.125 mM for malate (at pH 7.5 and at 70 degrees Celsius)</KM>
            <KM evidence="2">0.3 mM for malate (at pH 7.5 and at 70 degrees Celsius)</KM>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 8.0.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2 3">Optimum temperature is 85 degrees Celsius. At 95 degrees Celsius, the enzyme activity is still 66% of maximum, whereas at 40 degrees Celsius the enzyme is almost inactive. Thermostable.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK41339" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
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    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0816">Tricarboxylic acid cycle</keyword>
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<entry dataset="Swiss-Prot" created="2001-02-21" modified="2026-01-28" version="137" xmlns="https://uniprot.org/uniprot">
    <accession>P57766</accession>
    <name>PCNA1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA polymerase sliding clamp 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Proliferating cell nuclear antigen homolog 1</fullName>
            <shortName evidence="1">PCNA1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pcn1</name>
        <name type="synonym">pcnA-like</name>
        <name type="ordered locus">SSO0397</name>
        <name type="ORF">C41_008</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1999" name="J. Mol. Biol." volume="291" first="47" last="57">
            <title>Two DNA polymerase sliding clamps from the thermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="De Felice M."/>
                <person name="Sensen C.W."/>
                <person name="Charlebois R.L."/>
                <person name="Rossi M."/>
                <person name="Pisani F.M."/>
            </authorList>
            <dbReference type="PubMed" id="10438605"/>
            <dbReference type="DOI" id="10.1006/jmbi.1999.2939"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 1-10</scope>
        <scope>CHARACTERIZATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Mol. Cell" volume="11" first="275" last="282">
            <title>A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Dionne I."/>
                <person name="Nookala R.K."/>
                <person name="Jackson S.P."/>
                <person name="Doherty A.J."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="12535540"/>
            <dbReference type="DOI" id="10.1016/S1097-2765(02)00824-9"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH FEN AND PCNA2</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2003" name="Mol. Microbiol." volume="48" first="361" last="371">
            <title>An archaeal XPF repair endonuclease dependent on a heterotrimeric PCNA.</title>
            <authorList>
                <person name="Roberts J.A."/>
                <person name="Bell S.D."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="12675797"/>
            <dbReference type="DOI" id="10.1046/j.1365-2958.2003.03444.x"/>
        </citation>
        <scope>INTERACTION WITH XPF</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2006" name="J. Mol. Biol." volume="364" first="243" last="247">
            <title>PCNA activates the Holliday junction endonuclease Hjc.</title>
            <authorList>
                <person name="Dorazi R."/>
                <person name="Parker J.L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="17011573"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2006.09.011"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH HJC</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2006" name="Acta Crystallogr. F Struct. Biol. Commun." volume="62" first="944" last="948">
            <title>Structure of the heterotrimeric PCNA from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Williams G.J."/>
                <person name="Johnson K."/>
                <person name="Rudolf J."/>
                <person name="McMahon S.A."/>
                <person name="Carter L."/>
                <person name="Oke M."/>
                <person name="Liu H."/>
                <person name="Taylor G.L."/>
                <person name="White M.F."/>
                <person name="Naismith J.H."/>
            </authorList>
            <dbReference type="PubMed" id="17012780"/>
            <dbReference type="DOI" id="10.1107/S1744309106034075"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2006" name="Mol. Cell" volume="24" first="279" last="291">
            <title>A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA.</title>
            <authorList>
                <person name="Pascal J.M."/>
                <person name="Tsodikov O.V."/>
                <person name="Hura G.L."/>
                <person name="Song W."/>
                <person name="Cotner E.A."/>
                <person name="Classen S."/>
                <person name="Tomkinson A.E."/>
                <person name="Tainer J.A."/>
                <person name="Ellenberger T."/>
            </authorList>
            <dbReference type="PubMed" id="17052461"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2006.08.015"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.79 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2006" name="Nucleic Acids Res." volume="34" first="4515" last="4526">
            <title>Structure of an archaeal PCNA1-PCNA2-FEN1 complex: elucidating PCNA subunit and client enzyme specificity.</title>
            <authorList>
                <person name="Dore A.S."/>
                <person name="Kilkenny M.L."/>
                <person name="Jones S.A."/>
                <person name="Oliver A.W."/>
                <person name="Roe S.M."/>
                <person name="Bell S.D."/>
                <person name="Pearl L.H."/>
            </authorList>
            <dbReference type="PubMed" id="16945955"/>
            <dbReference type="DOI" id="10.1093/nar/gkl623"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) IN COMPLEX WITH PCNA2 AND FEN</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF 114-TYR--LYS-116 AND 175-LYS--TYR-177</scope>
    </reference>
    <reference key="9">
        <citation type="journal article" date="2008" name="Acta Crystallogr. D" volume="64" first="941" last="949">
            <title>Structures of monomeric, dimeric and trimeric PCNA: PCNA-ring assembly and opening.</title>
            <authorList>
                <person name="Hlinkova V."/>
                <person name="Xing G."/>
                <person name="Bauer J."/>
                <person name="Shin Y.J."/>
                <person name="Dionne I."/>
                <person name="Rajashankar K.R."/>
                <person name="Bell S.D."/>
                <person name="Ling H."/>
            </authorList>
            <dbReference type="PubMed" id="18703842"/>
            <dbReference type="DOI" id="10.1107/S0907444908021665"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 3-249</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="10">
        <citation type="journal article" date="2009" name="Mol. Microbiol." volume="71" first="678" last="691">
            <title>Structural insight into recruitment of translesion DNA polymerase Dpo4 to sliding clamp PCNA.</title>
            <authorList>
                <person name="Xing G."/>
                <person name="Kirouac K."/>
                <person name="Shin Y.J."/>
                <person name="Bell S.D."/>
                <person name="Ling H."/>
            </authorList>
            <dbReference type="PubMed" id="19054331"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.2008.06553.x"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 3-249 IN COMPLEX WITH DPO4</scope>
        <scope>INTERACTION WITH DPO4</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2 5">One of the sliding clamp subunits that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication. Heterotrimer stimulates the Holliday junction resolvase Hjc. DNA polymerase I, DNA ligase and the flap endonuclease may be constitutively associated with the PCNA heterotrimer forming a scanning complex able to couple DNA synthesis and Okazaki fragment maturation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3 4 5 6 7 8 9">Forms heterodimers with PCNA2, which then recruit PCNA3; does not form homotrimers (PubMed:12535540, PubMed:18703842). The heterodimers interact with RfcS homotetramers (PubMed:12535540). Heterotrimer which circularizes head-to-tail (head is at N-terminus, tail is at C-terminus) to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA. Heterotrimer interacts, probably via this subunit, with flap endonuclease 1 (fen) (PubMed:12535540), Hjc (PubMed:17011573), Dpo4 (PubMed:19054331), and XPF (PubMed:12675797).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PCNA family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90183">
        <property type="entry name" value="G90183"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988793.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2HII">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.79 A"/>
        <property type="chains" value="A/X=1-249"/>
    </dbReference>
    <dbReference type="PDB" id="2HIK">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.30 A"/>
        <property type="chains" value="A/L/X=1-249"/>
    </dbReference>
    <dbReference type="PDB" id="2IO4">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A/C=1-249"/>
    </dbReference>
    <dbReference type="PDB" id="2IX2">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A=1-249"/>
    </dbReference>
    <dbReference type="PDB" id="2IZO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="C=1-249"/>
    </dbReference>
    <dbReference type="PDB" id="2NTI">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/D/G=1-249"/>
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    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
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    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000149215" description="DNA polymerase sliding clamp 1">
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            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of interaction with PCNA3, no change with PCNA2." evidence="4">
        <original>YIK</original>
        <variation>ELE</variation>
        <location>
            <begin position="114"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of interaction with both PCNA3 and PCNA2." evidence="4">
        <original>KRY</original>
        <variation>EED</variation>
        <location>
            <begin position="175"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="3"/>
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    <feature type="helix" evidence="11">
        <location>
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    <evidence type="ECO:0000255" key="1">
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            <dbReference type="PubMed" id="17011573"/>
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            <dbReference type="PubMed" id="17012780"/>
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    </evidence>
    <evidence type="ECO:0007829" key="11">
        <source>
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    </evidence>
    <sequence length="249" mass="27536" checksum="137EB10BE2C03F77" modified="2001-02-21" version="1">MFKIVYPNAKDFFSFINSITNVTDSIILNFTEDGIFSRHLTEDKVLMAIMRIPKDVLSEYSIDSPTSVKLDVSSVKKILSKASSKKATIELTETDSGLKIIIRDEKSGAKSTIYIKAEKGQVEQLTEPKVNLAVNFTTDESVLNVIAADVTLVGEEMRISTEEDKIKIEAGEEGKRYVAFLMKDKPLKELSIDTSASSSYSAEMFKDAVKGLRGFSAPTMVSFGENLPMKIDVEAVSGGHMIFWIAPRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>P95872</accession>
    <name>RIB7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase</fullName>
            <shortName>DAROPP reductase</shortName>
            <shortName>DARP reductase</shortName>
            <ecNumber>1.1.1.302</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase</fullName>
            <shortName>DARIPP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2097</name>
        <name type="ORF">C06025</name>
        <name type="ORF">C06_008</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes an early step in riboflavin biosynthesis, the NADPH-dependent reduction of the ribose side chain of 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate, yielding 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NADP(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27278"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NAD(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27274"/>
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            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HTP reductase family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAA69508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42277.1"/>
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        <property type="component" value="Chromosome"/>
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        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
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        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
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        <property type="entry name" value="2,5-DIAMINO-6-RIBOSYLAMINO-4(3H)-PYRIMIDINONE 5'-PHOSPHATE REDUCTASE"/>
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        <property type="entry name" value="DIHYDROFOLATE REDUCTASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_8G06820)"/>
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    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="chain" id="PRO_0000135946" description="2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="79"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="145"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="213" mass="24561" checksum="A741F6CADEF7631C" modified="1997-05-01" version="1">MVMKPYVIIFSTVSIDGRLATKTGYSELSCPYDKQRQHEIRSEVDAVMVGANTVRVDNPSLTVKYGKNRRNPIRVVVTRSFNLDPSYKIFTTPPSTVIYTSNYESEKVEEFIRKGVIVRKFLHLDDLLEDLYDNFNVRRLMVEGGGHLIWWFIKDNLYDEIRITISPRIFGNGVSFTQGEGFIGEDSPRLELIDAKICECGNEVHLTYKKYMT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="142" xmlns="https://uniprot.org/uniprot">
    <accession>P95987</accession>
    <name>RS4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein uS4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">30S ribosomal protein S4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps4</name>
        <name evidence="6" type="synonym">rps4AB</name>
        <name type="ordered locus">SSO0073</name>
        <name evidence="4" type="ORF">C04_049</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="8 9 10" key="3">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Part of the 30S ribosomal subunit (PubMed:39753558). Contacts protein S5 (PubMed:39753558). The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="AAK40435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="S75415"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988885.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="D=1-181"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="D=1-181"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="D=1-181"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="D=1-181"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="D=1-181"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="D=1-181"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="D=1-181"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="D=1-181"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="P95987"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="P95987"/>
    <dbReference type="FunCoup" id="P95987">
        <property type="interactions" value="219"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0073"/>
    <dbReference type="PaxDb" id="273057-SSO0073"/>
    <dbReference type="EnsemblBacteria" id="AAK40435">
        <property type="protein sequence ID" value="AAK40435"/>
        <property type="gene ID" value="SSO0073"/>
    </dbReference>
    <dbReference type="GeneID" id="1455326"/>
    <dbReference type="KEGG" id="sso:SSO0073"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.74"/>
    <dbReference type="eggNOG" id="arCOG04239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089738_1_1_2"/>
    <dbReference type="InParanoid" id="P95987"/>
    <dbReference type="PhylomeDB" id="P95987"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000026">
        <property type="entry name" value="30S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_A">
        <property type="entry name" value="Ribosomal_uS4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022802">
        <property type="entry name" value="Ribosomal_uS4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
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        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding" evidence="1">
        <location>
            <begin position="104"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
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            <dbReference type="PubMed" id="39753558"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="PubMed" id="8899719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="7">
        <source>
            <dbReference type="EMBL" id="CAA69529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="10">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="181" mass="20748" checksum="E48B4C85CD98C522" modified="1997-05-01" version="1">MGDPKKSRKKWETPGHPWIKERIGYEQELLGKYGLRNKREIWIAQSIIRKFRHQARSLLALPPAERAVREKQLVGKLLKMGLLKKETATVDDILSLTEQDLLERRLQTIVYKKGLSNTIYQARQLITHGHIAVNGKRVTSPGYIVNVDEENLIDYYVTSSFKSRPPVMSQQEGGEIGVKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX65</accession>
    <name>Q7LX65_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sugar transport related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2134</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74046">
        <property type="entry name" value="S74046"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988328.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX65"/>
    <dbReference type="FunCoup" id="Q7LX65">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2134"/>
    <dbReference type="PaxDb" id="273057-SSO2134"/>
    <dbReference type="EnsemblBacteria" id="AAK42313">
        <property type="protein sequence ID" value="AAK42313"/>
        <property type="gene ID" value="SSO2134"/>
    </dbReference>
    <dbReference type="GeneID" id="1453640"/>
    <dbReference type="KEGG" id="sso:SSO2134"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2226"/>
    <dbReference type="eggNOG" id="arCOG02682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_2"/>
    <dbReference type="InParanoid" id="Q7LX65"/>
    <dbReference type="PhylomeDB" id="Q7LX65"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="15"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="423" mass="46443" checksum="CD7EE08B57CDDB62" modified="2011-05-31" version="1">MSYFDNIPLSVRIRTFIVTSAGFLLDGYDLNSISFAATIILKEFSLTTVKYGLLLAASLIGMIPGSIVFGWLSDKMGRSKIMGLDLFFFLVFGILTAISQNFVELFISRLLLGIGIGGDYPISSTLMSELSPSRSRGRYLTGSVAMYWVGVAISGIATLFLLPTGSYFWRYVFLIGALISVPIILLRLRLIESPRWLVSTGKANIKEINREIENKGVRGVVDLFKGKLLKITFFVTSVWFLFDVAAYGIGLYYPALLEEFAFPSKYEVVLGTLAIAAASVLGYIIAELLVDSLGRRVVLLVGLGFMTLLLYLGGIYKFTGGILVPYFMSFVALEQWAGAVTLFYPTELYPTPVRAIGQGFATAISRVGSVLGVFYFPILTKQMGFFNSLIMFGSVCLIAFIISILLSKETAKKPLEVTSEGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX66</accession>
    <name>Q7LX66_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transposase ISC1078</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1897</name>
        <name evidence="4" type="ordered locus">SSO2090</name>
        <name evidence="5" type="ordered locus">SSO2132</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74044">
        <property type="entry name" value="S74044"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923639.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX66"/>
    <dbReference type="STRING" id="273057.SSO1897"/>
    <dbReference type="PaxDb" id="273057-SSO1897"/>
    <dbReference type="EnsemblBacteria" id="AAK42087">
        <property type="protein sequence ID" value="AAK42087"/>
        <property type="gene ID" value="SSO1897"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK42269">
        <property type="protein sequence ID" value="AAK42269"/>
        <property type="gene ID" value="SSO2090"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK42311">
        <property type="protein sequence ID" value="AAK42311"/>
        <property type="gene ID" value="SSO2132"/>
    </dbReference>
    <dbReference type="GeneID" id="34442755"/>
    <dbReference type="KEGG" id="sso:SSO1897"/>
    <dbReference type="KEGG" id="sso:SSO2090"/>
    <dbReference type="KEGG" id="sso:SSO2132"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1952"/>
    <dbReference type="eggNOG" id="arCOG02127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056788_0_1_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025959">
        <property type="entry name" value="Winged_HTH_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13592">
        <property type="entry name" value="HTH_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Winged helix-turn helix" evidence="2">
        <location>
            <begin position="89"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="162"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="337" mass="39452" checksum="26E5C5B5271A33DA" modified="2011-05-31" version="1">MPKSRMHGIRLNKKQEEERRINAVKDVRNGMSIKDVAKKYDVSIFTVYKWLRKGDLSAKPRKGPTKLKDEEKLVRILEKSPRDFGLNYDFWTLKLIAYILDKEFGIKYNPRSLSPVLKKLGFKYKKGKRTYVRDDNAVERWVKEQGEKLFKKIREGYKVHIFDESYVSYINKGKGWMKVGGGIKVNPKRKRFAVVGGITISKEGITFSYSTYKKPSLNSKDIILYLRKVIKDEKTVIVMDNARIHGNEVREFLEENGVEFVYLPPYSPDKSPAEGPWAVLKKRLYSKIYTDFETLMWDATRFLRSISKRVNDLTYSVKRCGEPWTSILSEVFLPFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-03-27" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXH9</accession>
    <name>Q7LXH9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISC1476</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0794</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90229">
        <property type="entry name" value="E90229"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923087.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXH9"/>
    <dbReference type="PaxDb" id="273057-SSO0794"/>
    <dbReference type="DNASU" id="1455059"/>
    <dbReference type="EnsemblBacteria" id="AAK41092">
        <property type="protein sequence ID" value="AAK41092"/>
        <property type="gene ID" value="SSO0794"/>
    </dbReference>
    <dbReference type="GeneID" id="1455059"/>
    <dbReference type="KEGG" id="sso:SSO0794"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.800"/>
    <dbReference type="eggNOG" id="arCOG00683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040997_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LXH9"/>
    <dbReference type="PhylomeDB" id="Q7LXH9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="2">
        <location>
            <begin position="225"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="371" mass="43637" checksum="208E08043567DA5D" modified="2011-05-31" version="1">MAPSSGQLLGDEEREPTPTPAMPEEGVYEVKYSNRRTNVVRLLPNGFQERKLRRLANLSAKLFNEVNFERRQQFFHEGKVDFKGTWNKYYEKYKAILGVNAQAVLQKNNEAWSSFFSLLKLKKERKLPPHMNHVSPPRYWKDREAGKRKLILVVRQDRYKVDAGNHKLILKDFDMEIDFVGRLKWYGKQGRLEIIFDETKNAWYAHIPIEVGVEETGKKSKHVVKGERKSIQISKPKGNKVTSIDLGINVIASVVVNDGTWLLYKGVRTKEDYFYFQRRISEVQSLADRTRNIGEYDAYLELLREKRRFFKKLTRRLLHLYRNLASHLIETLHELGVSTIYLGYPFNIVQEKGNKFTVNMWSYRKLMVSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-03-27" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXJ8</accession>
    <name>Q7LXJ8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">THUMP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0773</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90226">
        <property type="entry name" value="F90226"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXJ8"/>
    <dbReference type="STRING" id="273057.SSO0773"/>
    <dbReference type="PaxDb" id="273057-SSO0773"/>
    <dbReference type="EnsemblBacteria" id="AAK41069">
        <property type="protein sequence ID" value="AAK41069"/>
        <property type="gene ID" value="SSO0773"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0773"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.772"/>
    <dbReference type="eggNOG" id="arCOG00084">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582937_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXJ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11717">
        <property type="entry name" value="THUMP_THUMPD1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040183">
        <property type="entry name" value="THUMPD1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="2">
        <location>
            <begin position="54"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="172" mass="19588" checksum="9556865295213800" modified="2011-05-31" version="1">MMWQNPKALVTTKPGKGRKCINEILNRILIKDIDARVIEYVQNVAIVYSNLDSLVVYGLLFASPPSCAEKVYPFQLIINSTNEKEIVVNVINFIKNKTKDLKTFYVRCYNRGIDVNCREIEMGVGIGLKGLINVDFKDPEVVLHVNVLKEFAGISLLRKNQEKFRATLLDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXL2</accession>
    <name>Q7LXL2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNase P subunit p30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90222">
        <property type="entry name" value="E90222"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991314.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXL2"/>
    <dbReference type="STRING" id="273057.SSO0740"/>
    <dbReference type="PaxDb" id="273057-SSO0740"/>
    <dbReference type="EnsemblBacteria" id="AAK41036">
        <property type="protein sequence ID" value="AAK41036"/>
        <property type="gene ID" value="SSO0740"/>
    </dbReference>
    <dbReference type="GeneID" id="1455003"/>
    <dbReference type="KEGG" id="sso:SSO0740"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.736"/>
    <dbReference type="eggNOG" id="arCOG00307">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1444601_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXL2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="21159" checksum="F1F4B6E755D111D5" modified="2011-05-31" version="1">MLIVESCILNSKLFPYAKRLGYNTIFSEDGLVGIKRVTIKVENGNQLKKAFKKQFVSKEVLVFVKPLSIDALKYAVINKRVNAVVLDDDNIRIFKKSMLNLLRSYGKFVEISLKSSSGLLYNAILFAYKWIPNIIFSSYASSFDEIWSPISKISYLTVLGADEEEAYKFVIINPIKLLNELNSINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXL6</accession>
    <name>Q7LXL6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">2-haloalkanoic acid dehalogenase, putative</fullName>
            <ecNumber evidence="6">3.8.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0726</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.8.1.2" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90221">
        <property type="entry name" value="A90221"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991291.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXL6"/>
    <dbReference type="FunCoup" id="Q7LXL6">
        <property type="interactions" value="132"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0726"/>
    <dbReference type="PaxDb" id="273057-SSO0726"/>
    <dbReference type="EnsemblBacteria" id="AAK41024">
        <property type="protein sequence ID" value="AAK41024"/>
        <property type="gene ID" value="SSO0726"/>
    </dbReference>
    <dbReference type="GeneID" id="1454991"/>
    <dbReference type="KEGG" id="sso:SSO0726"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.724"/>
    <dbReference type="eggNOG" id="arCOG02291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_8_3_2"/>
    <dbReference type="InParanoid" id="Q7LXL6"/>
    <dbReference type="PhylomeDB" id="Q7LXL6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018784">
        <property type="term" value="F:(S)-2-haloacid dehalogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051400">
        <property type="entry name" value="HAD-like_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470:SF2">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470">
        <property type="entry name" value="N-ACYLNEURAMINATE-9-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="242" mass="27690" checksum="85F39397DDE2C4A3" modified="2011-05-31" version="1">MSINYKLFNNVKAVLFDFDDTLVDFSSKAKEALDAVSKDIYTYIKENYQQEININIIRKLVEEESRKLDSQGEYNRNKWWENILKSLNIVHIDKSQLYDWTSLYWSIASQTEPYEDAKEIIEYLDSKGYKLGLVTNSDGEGGNKSSRLRTFPLIDKFDLILIAGEGGIRPKPNLEPFIISCEKLSVEPSSCVFVGDEPVKDCLAAKKANMISVLIDREGKVKDAELYADFVLSSLKQLEEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXN8</accession>
    <name>Q7LXN8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90214">
        <property type="entry name" value="G90214"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991217.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXN8"/>
    <dbReference type="STRING" id="273057.SSO0671"/>
    <dbReference type="PaxDb" id="273057-SSO0671"/>
    <dbReference type="EnsemblBacteria" id="AAK40974">
        <property type="protein sequence ID" value="AAK40974"/>
        <property type="gene ID" value="SSO0671"/>
    </dbReference>
    <dbReference type="GeneID" id="1454941"/>
    <dbReference type="KEGG" id="sso:SSO0671"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.672"/>
    <dbReference type="eggNOG" id="arCOG05893">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154342_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXN8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="137" mass="15109" checksum="C40747780614120F" modified="2011-05-31" version="1">MAHVNKQIRKRLVSISLGILLLTTSIFLLIKTGINSEQLQSALFFGISPILFYMLGIIFGLERIVFGVTGSEKLFRLLAGDGELYYTALLGVFFIFIISGVLILAYTPLIAGIIEKVLELINGLSFFALSATLFMRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXS2</accession>
    <name>Q7LXS2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-polyprenylphenol hydroxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0612</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90208">
        <property type="entry name" value="D90208"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991141.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXS2"/>
    <dbReference type="STRING" id="273057.SSO0612"/>
    <dbReference type="PaxDb" id="273057-SSO0612"/>
    <dbReference type="EnsemblBacteria" id="AAK40923">
        <property type="protein sequence ID" value="AAK40923"/>
        <property type="gene ID" value="SSO0612"/>
    </dbReference>
    <dbReference type="GeneID" id="1454890"/>
    <dbReference type="KEGG" id="sso:SSO0612"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.620"/>
    <dbReference type="eggNOG" id="arCOG02199">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1318587_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXS2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050353">
        <property type="entry name" value="PyrK_electron_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513:SF3">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="23647" checksum="53F690E56DC52CA4" modified="2011-05-31" version="1">MIYYEVIEKRKNVDNVYEIKIANFSVKPKAGQYVSLILPSKAEIPLGVGDYDEDNNELRLYVESEKLANEIGKRVILKGPLGKPLDFTNVRIIVGIAYGKLYHDLLYPLKVAYRSKIETFAKCIDCKLNYEEPKNIEDADLILVSAPLQLLYELKIPGEKTLVYNRWVKMNCNLGVCGLCEVKGKLTCIDGPFMRFDDLVDYGKGVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LYJ4</accession>
    <name>Q7LYJ4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Molybdenum cofactor biosynthesis protein E (MoaE)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">moaE</name>
        <name evidence="1" type="ordered locus">SSO2394</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90410">
        <property type="entry name" value="F90410"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989446.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LYJ4"/>
    <dbReference type="FunCoup" id="Q7LYJ4">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2394"/>
    <dbReference type="PaxDb" id="273057-SSO2394"/>
    <dbReference type="EnsemblBacteria" id="AAK42541">
        <property type="protein sequence ID" value="AAK42541"/>
        <property type="gene ID" value="SSO2394"/>
    </dbReference>
    <dbReference type="GeneID" id="1453868"/>
    <dbReference type="KEGG" id="sso:SSO2394"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2476"/>
    <dbReference type="eggNOG" id="arCOG00534">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG00536">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069141_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LYJ4"/>
    <dbReference type="PhylomeDB" id="Q7LYJ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00756">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00754">
        <property type="entry name" value="Ubl_MoaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.40">
        <property type="entry name" value="Molybdopterin biosynthesis MoaE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010038">
        <property type="entry name" value="MoaD_arc-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036563">
        <property type="entry name" value="MoaE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003448">
        <property type="entry name" value="Mopterin_biosynth_MoaE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01687">
        <property type="entry name" value="moaD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23404">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02391">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54690">
        <property type="entry name" value="Molybdopterin synthase subunit MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="231" mass="26189" checksum="594645E22EC315FE" modified="2011-05-31" version="1">MKKVKVLYFAFIKDITHKSNEVLETECETVDCFIEQLGKIYGSELVNFLKSGINGIKVSILVNGSASTKNIKDGDEVALLPPPSGGDLIIGKKFDLLEEIRKFREKAPPEAGSLVVYVGFVKGIVDNHRVFELKYEAYEEYTRKRFLEIKDEIKKKYNDLIEIEIIHVIDSMKPGENVLLIMAIGKGRKDAIDAVKETLELVKHSTGIWKLEIRDDGEYWVVAGNTRVKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TV3</accession>
    <name>Q97TV3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transposase ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1938</name>
        <name evidence="6" type="ordered locus">SSO2724</name>
        <name evidence="7" type="ordered locus">SSO3027</name>
        <name evidence="5" type="ordered locus">SSO3259</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90359">
        <property type="entry name" value="F90359"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TV3"/>
    <dbReference type="STRING" id="273057.SSO1938"/>
    <dbReference type="PaxDb" id="273057-SSO1938"/>
    <dbReference type="EnsemblBacteria" id="AAK42133">
        <property type="protein sequence ID" value="AAK42133"/>
        <property type="gene ID" value="SSO1938"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK42536">
        <property type="protein sequence ID" value="AAK42536"/>
        <property type="gene ID" value="SSO3259"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK42835">
        <property type="protein sequence ID" value="AAK42835"/>
        <property type="gene ID" value="SSO2724"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK43128">
        <property type="protein sequence ID" value="AAK43128"/>
        <property type="gene ID" value="SSO3027"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1938"/>
    <dbReference type="KEGG" id="sso:SSO2724"/>
    <dbReference type="KEGG" id="sso:SSO3027"/>
    <dbReference type="KEGG" id="sso:SSO3259"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2013"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074024_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025471">
        <property type="entry name" value="DUF4322"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033541">
        <property type="entry name" value="transpos_ISH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14210">
        <property type="entry name" value="DUF4322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4322" evidence="3">
        <location>
            <begin position="26"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="120"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="376" mass="43580" checksum="BD68542BA1A9F4FA" modified="2001-10-01" version="1">MTRTQLIEERFKVRKPVLSRGEDKVITPGLPHQNNLQQVGYKLLSMLSFKGRKAEEVSRVLVSACLWNDSVESKSKGYNVSPQTVRNYVEEQGTEVIEKLLESMRRISMEILKGVKEVDISIDWTTKTWYGKPVEGLGSSAKGNSWNYATATTKYQNMVLLLAFVPQVNGMSKDEIVKLLMEQIVGMGFKVGLVTLDAGFYTVEVLKFISQFKFVIGVPVGDVKIYEEFDGEYTTNSKRHKKEEQVKFRLLVYGKEIVKKRKKTVVYFARATNLDLPKREVLKLYNKVRSPIETSYRNIKAFLPFTSSTKFIFRELIFVLAMIFYSLYTVFKNVMTREEFRLLLILCFLDDLSDLKDFIFNLEETLINTIDLFLWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TV8</accession>
    <name>Q97TV8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposase ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1572</name>
        <name evidence="3" type="ordered locus">SSO2845</name>
        <name evidence="4" type="ordered locus">SSO3151</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90499">
        <property type="entry name" value="E90499"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923476.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TV8"/>
    <dbReference type="STRING" id="273057.SSO1572"/>
    <dbReference type="PaxDb" id="273057-SSO1572"/>
    <dbReference type="EnsemblBacteria" id="AAK41785">
        <property type="protein sequence ID" value="AAK41785"/>
        <property type="gene ID" value="SSO1572"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK42955">
        <property type="protein sequence ID" value="AAK42955"/>
        <property type="gene ID" value="SSO2845"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK43252">
        <property type="protein sequence ID" value="AAK43252"/>
        <property type="gene ID" value="SSO3151"/>
    </dbReference>
    <dbReference type="GeneID" id="1454564"/>
    <dbReference type="KEGG" id="sso:SSO1572"/>
    <dbReference type="KEGG" id="sso:SSO2845"/>
    <dbReference type="KEGG" id="sso:SSO3151"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1609"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="80"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37357" checksum="73FC83250C1348E8" modified="2001-10-01" version="1">MQTIQVSKTELKSLAINLATININVISQDLDPEIVKAAPSLLTGNRGKYYLKVVRQGEKVISKGQRTFKFYPIYREVKGETNVVAVDETGLTVGEKEQEKAEGFLLYNWKRKGIKMRSLDLVYPLRLPLLVEVADLRSDSPSQFLLRSVREVSQYMEIDYVVADAGFLNLGVIKEMPVKTIVRGKSNLKGFKELSNVPLVEKRYEVKDKVYVAYRVLEFEGLYYYDVVYVKGKPRHFMFVTNFEGDPYELAELYRLRWQVEEGFKVRKARIRYVRKLSNKIFLFLYYTVLDSAWNLVNHLLFNFKSTCKKVLSFDSFVKLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TZ9</accession>
    <name>HTPX2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protease HtpX homolog 2</fullName>
            <ecNumber evidence="1">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">htpX2</name>
        <name type="synonym">htpX-2</name>
        <name type="ordered locus">SSO3231</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M48B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90508">
        <property type="entry name" value="F90508"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989679.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TZ9"/>
    <dbReference type="FunCoup" id="Q97TZ9">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3231"/>
    <dbReference type="PaxDb" id="273057-SSO3231"/>
    <dbReference type="EnsemblBacteria" id="AAK43325">
        <property type="protein sequence ID" value="AAK43325"/>
        <property type="gene ID" value="SSO3231"/>
    </dbReference>
    <dbReference type="GeneID" id="1453244"/>
    <dbReference type="KEGG" id="sso:SSO3231"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3333"/>
    <dbReference type="eggNOG" id="arCOG01331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042266_4_1_2"/>
    <dbReference type="InParanoid" id="Q97TZ9"/>
    <dbReference type="PhylomeDB" id="Q97TZ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07338">
        <property type="entry name" value="M48B_HtpX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00188">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050083">
        <property type="entry name" value="HtpX_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022919">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002322">
        <property type="entry name" value="PRK01265.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221">
        <property type="entry name" value="PROTEASE HTPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221:SF2">
        <property type="entry name" value="PROTEASE HTPX HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000138931" description="Protease HtpX homolog 2">
        <location>
            <begin position="1"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00188"/>
        </source>
    </evidence>
    <sequence length="325" mass="36420" checksum="C71933866F60CE6C" modified="2001-10-01" version="1">MNWEVVKLRLNMALATLGITLLGFALALAVADYAFGAQFGVGLILSILIFIFFLNIIQWLFGPYMINWAYRTVEVTPTDPVYGWLYSTVAEVAKYNGFREVPKVYIADVPFPNAFAYGSPIAGKRIAFTLPILKLLNRDEIMAVAGHELGHLKHRDVELLMAVGLIPALIYYLGWWLFWGGLFSGGGNGRGNNGGLVFLLGIIMMAVSFVFQLLVLSLNRMREAYADVNSALTVPGGKENLQLALAKLTLSMDPEALERFKKKSTTNQMASMLFFTNAIEEVPTWNAKELVEIWKTTKVPWYADIFMDHPHPAKRIQLLDKVSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U38</accession>
    <name>Q97U38_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO12142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90503">
        <property type="entry name" value="E90503"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U38"/>
    <dbReference type="STRING" id="273057.SSO12142"/>
    <dbReference type="PaxDb" id="273057-SSO12142"/>
    <dbReference type="EnsemblBacteria" id="AAK43284">
        <property type="protein sequence ID" value="AAK43284"/>
        <property type="gene ID" value="SSO12142"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO12142"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3289"/>
    <dbReference type="eggNOG" id="arCOG03652">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2857280_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U38"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="64" mass="7001" checksum="3729B7C163F9FA1B" modified="2001-10-01" version="1">MELKRAVLSFKETYAQAMAVTAPLGSVVSTTTAAILYAGSSGYIHHVFILNNKCLMDLYLVDVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U83</accession>
    <name>Q97U83_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90497">
        <property type="entry name" value="G90497"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990916.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U83"/>
    <dbReference type="STRING" id="273057.SSO3140"/>
    <dbReference type="PaxDb" id="273057-SSO3140"/>
    <dbReference type="EnsemblBacteria" id="AAK43238">
        <property type="protein sequence ID" value="AAK43238"/>
        <property type="gene ID" value="SSO3140"/>
    </dbReference>
    <dbReference type="GeneID" id="1453157"/>
    <dbReference type="KEGG" id="sso:SSO3140"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3244"/>
    <dbReference type="eggNOG" id="arCOG06002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_511559_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U83"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="550" mass="61209" checksum="823ECE2855D61CD3" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UA8</accession>
    <name>Q97UA8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF998 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90494">
        <property type="entry name" value="F90494"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990933.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UA8"/>
    <dbReference type="STRING" id="273057.SSO3111"/>
    <dbReference type="PaxDb" id="273057-SSO3111"/>
    <dbReference type="EnsemblBacteria" id="AAK43213">
        <property type="protein sequence ID" value="AAK43213"/>
        <property type="gene ID" value="SSO3111"/>
    </dbReference>
    <dbReference type="GeneID" id="1453132"/>
    <dbReference type="KEGG" id="sso:SSO3111"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3217"/>
    <dbReference type="eggNOG" id="arCOG02008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080422_2_0_2"/>
    <dbReference type="InParanoid" id="Q97UA8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009339">
        <property type="entry name" value="DUF998"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06197">
        <property type="entry name" value="DUF998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="19043" checksum="E13016C38B29464C" modified="2001-10-01" version="1">MMLNRTKVSGYLILVGVSQFLLFFIISEILYPNYSVKYNYISDLGVGKTAIIFNTSIVIMGILVIIASILLRANYSPLVFLVGLGAALVGIFPENTGLPHLIASLITFLFGGIGALVTSIKRNYFWGILGIATLVSLILFILKDYGPLGPGGLERMIVYPEIIWGISFATYLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UF0</accession>
    <name>Q97UF0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dihydrodipicolinate synthase (DapA-3)</fullName>
            <ecNumber evidence="4">4.3.3.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dapA-3</name>
        <name evidence="4" type="ordered locus">SSO3072</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.3.3.7" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90489">
        <property type="entry name" value="D90489"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990871.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UF0"/>
    <dbReference type="FunCoup" id="Q97UF0">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3072"/>
    <dbReference type="PaxDb" id="273057-SSO3072"/>
    <dbReference type="EnsemblBacteria" id="AAK43171">
        <property type="protein sequence ID" value="AAK43171"/>
        <property type="gene ID" value="SSO3072"/>
    </dbReference>
    <dbReference type="GeneID" id="7808682"/>
    <dbReference type="KEGG" id="sso:SSO3072"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3171"/>
    <dbReference type="eggNOG" id="arCOG04172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_5_1_2"/>
    <dbReference type="InParanoid" id="Q97UF0"/>
    <dbReference type="PhylomeDB" id="Q97UF0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008675">
        <property type="term" value="F:2-dehydro-3-deoxy-phosphogluconate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00408">
        <property type="entry name" value="DHDPS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128:SF66">
        <property type="entry name" value="4-HYDROXY-2-OXOGLUTARATE ALDOLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="DIHYDRODIPICOLINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="2">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="302" mass="34099" checksum="CC825E146E4BEE51" modified="2001-10-01" version="1">MRLEGVVVPLITPFLEDYSIDKEGLKWLVSYLSENGVNGIFVNSSVGEFASLTLDEMKLVTKIALDSRKSYSTLIFAGASTNFTEETIKLAKDFKDMGVDAIVVMAPYFFKVREKELLDHFSMIAEKVDLPIIIYNIPLFTGIDIPISVYKMLVSQHSNIIGTKVTLDSLIFFKKLISEIREIRKDFSILTGFDEYLLPLLYMGGNGGVMGLANAIPKLHLKVYESWKNGNLSDANKYWRNVLNLTDIYDYCNSYTASIKLLLKVLNMPIKNVVRPPLSICEEESKIRGMIKDLSSFLTNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UH3</accession>
    <name>Q97UH3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter, ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO3046</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90486">
        <property type="entry name" value="C90486"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924109.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UH3"/>
    <dbReference type="FunCoup" id="Q97UH3">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3046"/>
    <dbReference type="PaxDb" id="273057-SSO3046"/>
    <dbReference type="EnsemblBacteria" id="AAK43146">
        <property type="protein sequence ID" value="AAK43146"/>
        <property type="gene ID" value="SSO3046"/>
    </dbReference>
    <dbReference type="GeneID" id="1453065"/>
    <dbReference type="KEGG" id="sso:SSO3046"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3143"/>
    <dbReference type="eggNOG" id="arCOG05410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="InParanoid" id="Q97UH3"/>
    <dbReference type="PhylomeDB" id="Q97UH3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067:SF3">
        <property type="entry name" value="MALTOSE ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067">
        <property type="entry name" value="OLIGOPEPTIDE/DIPEPTIDE ABC TRANSPORTER, ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="8"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="331" mass="37342" checksum="605E12AF6CDB52FC" modified="2001-10-01" version="1">MSEISISVKNLNVGYLSDYGIIKVLRNVSIDIPESKIIGVAGESGSGKSTLATTIMGFVNPPLYVERGSIIVDSKYDILSMSRDELNKIRGSVISYVPQAAQNSLNPIRKVKETFTDILKAKGMDYYANIDVVYNTLEEVGLTDRQVLDMYPFQLSGGMKQRVVIAISLLLNPKILIMDEPTTGLDVVVQYEILKLLKKIQKEKDLTLVVISHDIAMLFQISDYIAVMYGGQIVEYGKYNTLLEEAYHPYTYLLLSSVPTVRSRGKKLPRIPGDFEGFIRYPKGCVFSSRCPFVTSTCKEVPPQIVAYDKFGFYKCIRYPEWRNEVKKAYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UI9</accession>
    <name>Q97UI9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">One of two inversely orientated ORFs in ISC1043</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3026</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90483">
        <property type="entry name" value="H90483"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UI9"/>
    <dbReference type="PaxDb" id="273057-SSO3026"/>
    <dbReference type="EnsemblBacteria" id="AAK43127">
        <property type="protein sequence ID" value="AAK43127"/>
        <property type="gene ID" value="SSO3026"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3026"/>
    <dbReference type="HOGENOM" id="CLU_2178006_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UI9"/>
    <dbReference type="PhylomeDB" id="Q97UI9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="109" mass="12596" checksum="44ADD7B33B86C6B3" modified="2001-10-01" version="1">MNFNIISLHLFPPLIVALGYQVDLRIREVIQYRLAIVLVSPQGVNLYTKIPQDLENHLISLPLTLKRITVSDNQVTTQVKDEEGVRELLPFLHIFPRLLPFTPLMLGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UJ7</accession>
    <name>Q97UJ7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO3014</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90482">
        <property type="entry name" value="E90482"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UJ7"/>
    <dbReference type="FunCoup" id="Q97UJ7">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3014"/>
    <dbReference type="PaxDb" id="273057-SSO3014"/>
    <dbReference type="EnsemblBacteria" id="AAK43116">
        <property type="protein sequence ID" value="AAK43116"/>
        <property type="gene ID" value="SSO3014"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3014"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3106"/>
    <dbReference type="eggNOG" id="arCOG01617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_2"/>
    <dbReference type="InParanoid" id="Q97UJ7"/>
    <dbReference type="PhylomeDB" id="Q97UJ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19079">
        <property type="entry name" value="AKR_EcYajO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000004">
        <property type="entry name" value="Oxidoreductase, aldo/keto reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF4">
        <property type="entry name" value="NAD(P)-LINKED OXIDOREDUCTASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="25"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="344" mass="39281" checksum="81DF4D7136837FC2" modified="2001-10-01" version="1">MCSRFIHMKYVNLGESGVKVSQVAIGTWFLPRLQEKDELGIYKVDKETTLKILKRAYDEGVNFIDTANRYHGAMAPVDLPHVGNAERIVGEFLKTVDRESVVISTKVRGKMGDFVNGEGLSRKHILWQIKESLKRLGTSYIDVYFIHWPDPDTPKLETLRTLNSLVNNGLVYYLGVSNHSAVDVMEFLQLSERHNLEKFIVMQEKYNMLERDIEKDKAIIAKRFGLAIMAYSPLAQGFLTGKYVDKNGWKIEELSRASITEDLKKRYFTEANLRILLGLKDVASELGITLSQLAISWLLKRGEQLGITVIPLIGASKIEHLEDNLSALNINLRDDYMKRIEQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UK2</accession>
    <name>Q97UK2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbon monoxide dehydrogenase, large chain (CutA-7)</fullName>
            <ecNumber evidence="4">1.2.7.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cutA-7</name>
        <name evidence="4" type="ordered locus">SSO3009</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.4" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90481">
        <property type="entry name" value="H90481"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992661.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UK2"/>
    <dbReference type="SMR" id="Q97UK2"/>
    <dbReference type="FunCoup" id="Q97UK2">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3009"/>
    <dbReference type="PaxDb" id="273057-SSO3009"/>
    <dbReference type="EnsemblBacteria" id="AAK43111">
        <property type="protein sequence ID" value="AAK43111"/>
        <property type="gene ID" value="SSO3009"/>
    </dbReference>
    <dbReference type="GeneID" id="1453030"/>
    <dbReference type="KEGG" id="sso:SSO3009"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3100"/>
    <dbReference type="eggNOG" id="arCOG01167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_2_0_2"/>
    <dbReference type="InParanoid" id="Q97UK2"/>
    <dbReference type="PhylomeDB" id="Q97UK2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:anaerobic carbon-monoxide dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.50">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.365.10">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046867">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908:SF132">
        <property type="entry name" value="ALDEHYDE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="MoCoBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20256">
        <property type="entry name" value="MoCoBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde oxidase/xanthine dehydrogenase a/b hammerhead" evidence="3">
        <location>
            <begin position="20"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="723" mass="79542" checksum="E8C59A6262525EA3" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UL0</accession>
    <name>Q97UL0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Manganese transporter, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2995</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90480">
        <property type="entry name" value="F90480"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992646.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UL0"/>
    <dbReference type="FunCoup" id="Q97UL0">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2995"/>
    <dbReference type="PaxDb" id="273057-SSO2995"/>
    <dbReference type="EnsemblBacteria" id="AAK43101">
        <property type="protein sequence ID" value="AAK43101"/>
        <property type="gene ID" value="SSO2995"/>
    </dbReference>
    <dbReference type="GeneID" id="1453020"/>
    <dbReference type="KEGG" id="sso:SSO2995"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3090"/>
    <dbReference type="eggNOG" id="arCOG04531">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020088_6_0_2"/>
    <dbReference type="InParanoid" id="Q97UL0"/>
    <dbReference type="PhylomeDB" id="Q97UL0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006828">
        <property type="term" value="P:manganese ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF33">
        <property type="entry name" value="NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="394" mass="43182" checksum="E6790F856E1A7289" modified="2001-10-01" version="1">MSIKEVIRLFGPAWIVMMADVDAASVLTGVANGQEYGYRLIWLLLLLSIPLYIIQEAAGRLGAVNNGKGLGEIIRERYSGKIALLASLSMFVVDVFTYIVEYVGIAVGGLVLGIPPYITLPIFFVFHLVIISTRKYEKIEKFLLPISLLLAVAFVLQAMLRGIVNGENVLYFSPSKSFTFLVAANIGAVIMPFMIFYQTSATSYKYQNVESSIETKIKWSSIETLIGAIVSELIMVAIEMATTGISPSVDPLNYKEISYALSLVSGPFSPYIFGIGLICAAFLALIVESLGSAWGTLEALGKNSFNNFILLYISESIPALVITLLFTNNYDNVVNFALTLMSIAPFIVVIPCVLIGMLVKDKNLMMNYAYGRTRMLVYWITVAMILIGGILAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UM5</accession>
    <name>Q97UM5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">S-formylglutathione hydrolase</fullName>
            <ecNumber evidence="2">3.1.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2979</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the esterase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.12" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90478">
        <property type="entry name" value="D90478"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992578.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UM5"/>
    <dbReference type="STRING" id="273057.SSO2979"/>
    <dbReference type="ESTHER" id="sulso-SSO2979">
        <property type="family name" value="A85-Feruloyl-Esterase"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2979"/>
    <dbReference type="EnsemblBacteria" id="AAK43083">
        <property type="protein sequence ID" value="AAK43083"/>
        <property type="gene ID" value="SSO2979"/>
    </dbReference>
    <dbReference type="GeneID" id="1453002"/>
    <dbReference type="KEGG" id="sso:SSO2979"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3070"/>
    <dbReference type="eggNOG" id="arCOG05356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037618_0_1_2"/>
    <dbReference type="InParanoid" id="Q97UM5"/>
    <dbReference type="PhylomeDB" id="Q97UM5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018738">
        <property type="term" value="F:S-formylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014186">
        <property type="entry name" value="S-formylglutathione_hydrol"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061:SF0">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0719">Serine esterase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="320" mass="37317" checksum="11A121C634779A0D" modified="2001-10-01" version="1">MNLTIIEFESNVLRDNPLNDPYKRRVGIIYPKDYEGRPILIYLSGYLSSSLTQINYNPLGEDMVSKVERLSNEGKMKGSVIVLPDMFTKVGGNQYINSSAVGMYEDFLVKELIPFLKDKFKSDKIGIFGHSSGGYGALILGMKYPNTIKAIADHAGDAYFEYVYLPTFPRAIEQLRRFKTPEEWLENYWKKENKQHREDLNTLNVVGMSAFYSPNNEKIELPFDLETGEILEDVWKKWLEKDPVRMVDKYADNLKMLKFIFIDVGKKDEFNIQYGSRTLHKKLQKYGINHYYEEFNDGHLHTNYRYDISLSLLEKKLTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UN9</accession>
    <name>Q97UN9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO11575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90476">
        <property type="entry name" value="F90476"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UN9"/>
    <dbReference type="PaxDb" id="273057-SSO11575"/>
    <dbReference type="EnsemblBacteria" id="AAK43069">
        <property type="protein sequence ID" value="AAK43069"/>
        <property type="gene ID" value="SSO11575"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11575"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3055"/>
    <dbReference type="eggNOG" id="arCOG08505">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2784278_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UN9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="69" mass="8134" checksum="90D6832C2C482449" modified="2001-10-01" version="1">MMKVVELKKIYSQILSLSEECKKCVDCEACDRVEELIDYVIENIDKMPIEEQTEARRILNSILTLRKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UR2</accession>
    <name>Q97UR2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2940</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AAK43044.1"/>
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    </dbReference>
    <dbReference type="STRING" id="273057.SSO2940"/>
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    <dbReference type="EnsemblBacteria" id="AAK43044">
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        <property type="gene ID" value="SSO2940"/>
    </dbReference>
    <dbReference type="GeneID" id="1452963"/>
    <dbReference type="KEGG" id="sso:SSO2940"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3028"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_030571_2_6_2"/>
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        <property type="component" value="Chromosome"/>
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        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
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    </dbReference>
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        <property type="entry name" value="Metallo-B-lactamas"/>
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        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
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        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF18">
        <property type="entry name" value="METALLO-HYDROLASE MJ0296-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="2">
        <location>
            <begin position="21"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="235" mass="26630" checksum="7896EF0F327547BA" modified="2001-10-01" version="1">MNWKILVNGVPIYTSLGFVGFCNIVLMDIDDKYLIYDPGHFGNREVLLKALKDSGLSPLDIDGVILSHMHYDHSLNSLLFPNAKVYTTREEVEYTKNTPDNYSVTYLPDLLRERIVLVKNNEQLDGLKFILLPGHTGGSLGVMYKDTIFVGDAIKYIIDAKNGETSFAYYDITEANKSIRKVLKLANKVVPGHDAPFTIVRDSNKIEIIPDSDLINKEFTVYTRSDEPKILIRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UR4</accession>
    <name>Q97UR4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Permease (Proline/betaine)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2938</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90473">
        <property type="entry name" value="C90473"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UR4"/>
    <dbReference type="FunCoup" id="Q97UR4">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2938"/>
    <dbReference type="EnsemblBacteria" id="AAK43042">
        <property type="protein sequence ID" value="AAK43042"/>
        <property type="gene ID" value="SSO2938"/>
    </dbReference>
    <dbReference type="GeneID" id="1452961"/>
    <dbReference type="KEGG" id="sso:SSO2938"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3026"/>
    <dbReference type="eggNOG" id="arCOG02691">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_2_2"/>
    <dbReference type="InParanoid" id="Q97UR4"/>
    <dbReference type="PhylomeDB" id="Q97UR4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045:SF1">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="8"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK43042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="438" mass="48554" checksum="AF6A182B55EE5183" modified="2001-10-01" version="1">MVSEKTKAIIASSFGMALEWYDFFIYTFVATVVSKLFFPSSNPIASLLAFYLTFFIGFLGRPLGGIVFGYLGDKVGRKYSLVFTILLTGLSVFFVGLLPTYYQIGLLAPILLAILRFLTGVALGGEWGGAFSLTSEYVNPGRRGLYSGILQATVSIANLLATGIIFVIIIIMGQTGFDNFGWRILFYSGLLIAIVGLLIRLRIEDSPVFRKLREDRKILKNPISEAFRKYWKLILANLFIVGIINGAWYYTNFAYSVGYATTIAQRFNMPHVSLQTIDFAIFLVSAIGIFASIFFGYLSDIIGRRTQIIINSIMGIGLAYPYYYLLLQGNESAVIGAIFLGGLLIYYFAGAITPAFLVESFPPMVRYTAISFSYQVGVGFIGGLTPFILTYLVSATSNIFSPVYFTILTGIVVLILALLLKETKGRIYEGEEILKQEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97US5</accession>
    <name>Q97US5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90471">
        <property type="entry name" value="D90471"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924035.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97US5"/>
    <dbReference type="STRING" id="273057.SSO2918"/>
    <dbReference type="PaxDb" id="273057-SSO2918"/>
    <dbReference type="EnsemblBacteria" id="AAK43027">
        <property type="protein sequence ID" value="AAK43027"/>
        <property type="gene ID" value="SSO2918"/>
    </dbReference>
    <dbReference type="GeneID" id="1452946"/>
    <dbReference type="KEGG" id="sso:SSO2918"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3008"/>
    <dbReference type="eggNOG" id="arCOG06508">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134214_0_0_2"/>
    <dbReference type="InParanoid" id="Q97US5"/>
    <dbReference type="PhylomeDB" id="Q97US5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase InsH N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="128" mass="14987" checksum="7AD3AF731293DDBE" modified="2001-10-01" version="1">MDKSFPWETFRSKLKSLYSKKPKWDVISLLKVLLIKFVFDISWNNLEGEIRDSKSFMDFLGGKVPPKSTVFFFYKKLQQTVIQEGETMRTTLMDELNKALDKAISEYREKGFELEVGREKTIGSRTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UU2</accession>
    <name>Q97UU2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LmbE family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2901</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90469">
        <property type="entry name" value="C90469"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UU2"/>
    <dbReference type="STRING" id="273057.SSO2901"/>
    <dbReference type="PaxDb" id="273057-SSO2901"/>
    <dbReference type="EnsemblBacteria" id="AAK43010">
        <property type="protein sequence ID" value="AAK43010"/>
        <property type="gene ID" value="SSO2901"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2901"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2991"/>
    <dbReference type="eggNOG" id="arCOG03460">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049311_0_1_2"/>
    <dbReference type="InParanoid" id="Q97UU2"/>
    <dbReference type="PhylomeDB" id="Q97UU2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993:SF11">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="22185" checksum="99069CE2DA11EDC0" modified="2001-10-01" version="1">MQKSSCMRRVLIVAPHPDDETLCCGGTIQIFKEKGYKISVIIVTDGRYGSPDDKLKGSSELIEIRRQEALRATKILGIDEVKFLNFEDSKVSEEDAENALAEFLRENDVVFSPIPFDNHPDHANIGKAVEKLYPNAYFYLIWGNTQVNWREVKFDIRKYKESKLRAINQYISQIGGLPLERFTGDYEVFWTKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UU6</accession>
    <name>Q97UU6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH hxlR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2897</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90468">
        <property type="entry name" value="E90468"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990687.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UU6"/>
    <dbReference type="SMR" id="Q97UU6"/>
    <dbReference type="STRING" id="273057.SSO2897"/>
    <dbReference type="PaxDb" id="273057-SSO2897"/>
    <dbReference type="EnsemblBacteria" id="AAK43004">
        <property type="protein sequence ID" value="AAK43004"/>
        <property type="gene ID" value="SSO2897"/>
    </dbReference>
    <dbReference type="GeneID" id="1452923"/>
    <dbReference type="KEGG" id="sso:SSO2897"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2984"/>
    <dbReference type="eggNOG" id="arCOG01057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111585_5_2_2"/>
    <dbReference type="InParanoid" id="Q97UU6"/>
    <dbReference type="PhylomeDB" id="Q97UU6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33204">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, MARR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33204:SF18">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="4">
        <location>
            <begin position="13"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="127" mass="14413" checksum="F72F278D5781906F" modified="2001-10-01" version="1">MVKRNKVIKQQSCPLIDTINVISRKWLLLVLNTIGNEGKLGFNSILNSIDDISPKALSDVLKTMEEMGLVKKVIVKSSPPRVEYSLTYEGKKLRKAVIPLFKWAAEHTGHYECPILQSNKLNKEEYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V31</accession>
    <name>Q97V31_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Rieske iron-sulfur protein-1 (SoxL-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">soxL-2</name>
        <name evidence="9" type="ordered locus">SSO2803</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90457">
        <property type="entry name" value="E90457"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991446.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V31"/>
    <dbReference type="STRING" id="273057.SSO2803"/>
    <dbReference type="PaxDb" id="273057-SSO2803"/>
    <dbReference type="EnsemblBacteria" id="AAK42916">
        <property type="protein sequence ID" value="AAK42916"/>
        <property type="gene ID" value="SSO2803"/>
    </dbReference>
    <dbReference type="GeneID" id="1452835"/>
    <dbReference type="KEGG" id="sso:SSO2803"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2889"/>
    <dbReference type="eggNOG" id="arCOG01720">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074282_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V31"/>
    <dbReference type="PhylomeDB" id="Q97V31"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03475">
        <property type="entry name" value="Rieske_SoxF_SoxL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017586">
        <property type="entry name" value="Rieske_Fe-S_SoxL2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03171">
        <property type="entry name" value="soxL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Rieske" evidence="8">
        <location>
            <begin position="173"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="290"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="322" mass="34631" checksum="AA966565E10EBC9D" modified="2001-10-01" version="1">MIRFKSDKEDKPILEYPDLAFIRKLIRKMRDPKTKFDAKEFVNKGEDYLFNYTNKNVGSVDEKRRKFLKSLIFGIAAAAVVGVIPGLKVLVPPTVTVTSGFPKSLLVDSSGSPIKASEIPVNSPFIIIFEYPMTGEPNFLINLGDSTGKPIEIPSTSVVVPQTGKTYTFPGGVGPNKSIVAYSAICQHLGCTPPYIHFYPPQYVGPSQLTASEPDQLTAAALLAAKQAKVPALIHCDCHGSTYDPYHGAAVLTGPTVRPLPAVVLEWDPSTDYLYAIGAVGVATYPNGSNGVPTSNPTDDLSSSFGSSVSDKTTVQETENPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V38</accession>
    <name>Q97V38_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oxydoreductase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2794</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90456">
        <property type="entry name" value="D90456"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V38"/>
    <dbReference type="SMR" id="Q97V38"/>
    <dbReference type="STRING" id="273057.SSO2794"/>
    <dbReference type="PaxDb" id="273057-SSO2794"/>
    <dbReference type="EnsemblBacteria" id="AAK42907">
        <property type="protein sequence ID" value="AAK42907"/>
        <property type="gene ID" value="SSO2794"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2794"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2880"/>
    <dbReference type="eggNOG" id="arCOG01500">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_1_1_2"/>
    <dbReference type="InParanoid" id="Q97V38"/>
    <dbReference type="PhylomeDB" id="Q97V38"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042279">
        <property type="term" value="F:nitrite reductase (cytochrome, ammonia-forming) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10551">
        <property type="entry name" value="PsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050954">
        <property type="entry name" value="ET_IronSulfur_Cluster-Binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43177">
        <property type="entry name" value="PROTEIN NRFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43177:SF3">
        <property type="entry name" value="PROTEIN NRFC HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="46"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="103"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="138"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="293" mass="32382" checksum="C13A04963619EDAD" modified="2001-10-01" version="1">MGSQQNPPLKVINMSSPQNTTTPQIKLDIRQGGVQHAPPFSLKANYAIITDLNKCFGCGGCQMSCKEWNTSGMFGPLPDLNPYGELDVMFWLRVLYVEVGTYPQTKVYNIPINCFHCINAPCTEVCPVGATFKRTEDGIVLVDYNECIGTKYCIYACPYGNRFFDYVEGVTKKCTHCFDRIYDPTLPPEERIPACIHGCMVQARIWANILDPTDPGTILFYDKGGFVMGPETGAQPASGYLPWRSKYAESDVQLLSEAEYYNVWTANGVVQLGAQGNNSTYTEGNGSNSSSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V46</accession>
    <name>Q97V46_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mechanosensitive ion channel MscS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2786</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90455">
        <property type="entry name" value="D90455"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991934.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V46"/>
    <dbReference type="STRING" id="273057.SSO2786"/>
    <dbReference type="PaxDb" id="273057-SSO2786"/>
    <dbReference type="EnsemblBacteria" id="AAK42899">
        <property type="protein sequence ID" value="AAK42899"/>
        <property type="gene ID" value="SSO2786"/>
    </dbReference>
    <dbReference type="GeneID" id="1452818"/>
    <dbReference type="KEGG" id="sso:SSO2786"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2870"/>
    <dbReference type="eggNOG" id="arCOG01573">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115707_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V46"/>
    <dbReference type="PhylomeDB" id="Q97V46"/>
    <dbReference type="BRENDA" id="6.3.4.14">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221:SF1">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="6">
        <location>
            <begin position="134"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="205" mass="21801" checksum="5F29B8D66FF9D651" modified="2001-10-01" version="1">MAKSTTTKVGNAIVRTIIYIILYVILSAIVKYIIESLLPSFNIHAVSYESYVQILLALAFGYLIVSGISLIFYYSLLPKYGHPTAAAVRNIMRIIGIGALAASIAGAVAGGAAGVALGGFIGIVIGFASQQVLGQAVAGLFLLVARPFKVNDNVNIVSEDGIVEDVSTLFTTILKADGTRVLIPNNMIIGNKIYLKQTEQQQKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V61</accession>
    <name>Q97V61_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycosyltransferase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2775</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90453">
        <property type="entry name" value="E90453"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990224.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V61"/>
    <dbReference type="FunCoup" id="Q97V61">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2775"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2775"/>
    <dbReference type="DNASU" id="1452803"/>
    <dbReference type="EnsemblBacteria" id="AAK42884">
        <property type="protein sequence ID" value="AAK42884"/>
        <property type="gene ID" value="SSO2775"/>
    </dbReference>
    <dbReference type="GeneID" id="1452803"/>
    <dbReference type="KEGG" id="sso:SSO2775"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2856"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_2_2"/>
    <dbReference type="InParanoid" id="Q97V61"/>
    <dbReference type="PhylomeDB" id="Q97V61"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="3">
        <location>
            <begin position="17"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="173"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="349" mass="40014" checksum="F0A7983D8090ACD6" modified="2001-10-01" version="1">MDLLITNHRDLRHRKAGGAEQVIYEISRRLVKKGVSVTWLSEEIKGLPKEEEIENIRIIRRGNSITLHLYAPIEARRHEIVIDSVAHAVPFYSYLVNKNAIALVHHVHQDVVKYELNPIMARAVAFSERSVSNYKSIIAVSNTTKRDLVERLKAKEDRIEVIHNGIDHNIYRPGKKASEPTILWIGRLKKYKNPLDAIDVAKRVKAKLVMAGGGDLENEVVQKIKTINGEYLGRVSEEDKIRLYQSAWIVIVTSFIEGWSMVTVEANACGTPVIAYNKGSLPEIIKNGVNGYIVNYKDIEAMSKIINELIEDEKRIKELWKSSYEESLNYDWDKSSEKYYNYLIRKALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V66</accession>
    <name>Q97V66_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mechanosensitive ion channel MscS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2769</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90452">
        <property type="entry name" value="H90452"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990233.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V66"/>
    <dbReference type="STRING" id="273057.SSO2769"/>
    <dbReference type="PaxDb" id="273057-SSO2769"/>
    <dbReference type="EnsemblBacteria" id="AAK42879">
        <property type="protein sequence ID" value="AAK42879"/>
        <property type="gene ID" value="SSO2769"/>
    </dbReference>
    <dbReference type="GeneID" id="1452798"/>
    <dbReference type="KEGG" id="sso:SSO2769"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2851"/>
    <dbReference type="eggNOG" id="arCOG01568">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074283_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V66"/>
    <dbReference type="PhylomeDB" id="Q97V66"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221:SF1">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="6">
        <location>
            <begin position="124"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="308" mass="34031" checksum="3B96FC2CC69CA9DA" modified="2001-10-01" version="1">MALNERRVIALILTVVLAGIALGGGVFVLSSVFKIIPADYLLYIYAAIWFIVAVIVSYFIGSLIRNRLGDVIGYDNASSISFVVRLIGYIIAFVGFFVLIRVSLGTALAAGGFAGLVLGLAAQDVLANIFGGIMIVISRPYKVGDRVTVSTWQYGLLAPTYPPKFFSNDFLIPGYTGTVTDISLLYTTIFTDDQIPVKIPNSIMIQAAIFIHNGEEKRKVRTKYEVSKDIDPDVLINVLKEKIKKLDFVIEEPSIKVLETTLNTYILGIDTICKTIYEEPPRSEILKIVMRTVRELQTKTAAASDYNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V92</accession>
    <name>Q97V92_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2743</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA damage repair.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nre family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90449">
        <property type="entry name" value="F90449"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990272.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V92"/>
    <dbReference type="STRING" id="273057.SSO2743"/>
    <dbReference type="PaxDb" id="273057-SSO2743"/>
    <dbReference type="EnsemblBacteria" id="AAK42853">
        <property type="protein sequence ID" value="AAK42853"/>
        <property type="gene ID" value="SSO2743"/>
    </dbReference>
    <dbReference type="GeneID" id="1452772"/>
    <dbReference type="KEGG" id="sso:SSO2743"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2822"/>
    <dbReference type="eggNOG" id="arCOG04269">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039703_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V92"/>
    <dbReference type="PhylomeDB" id="Q97V92"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02096">
        <property type="entry name" value="Nre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033167">
        <property type="entry name" value="Nre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006979">
        <property type="entry name" value="Nre_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006978">
        <property type="entry name" value="Nre_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38136">
        <property type="entry name" value="DNA REPAIR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38136:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04895">
        <property type="entry name" value="Nre_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04894">
        <property type="entry name" value="Nre_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Archaeal Nre N-terminal" evidence="2">
        <location>
            <begin position="16"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Archaeal Nre C-terminal" evidence="3">
        <location>
            <begin position="291"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="397" mass="45451" checksum="0F0CF1EEC1674072" modified="2001-10-01" version="1">MIRPELCIRCRGAKYLCGLSYCPIIVNTKTVRVNFKEVYGSSPPSVFVGRFSYPKITVYPSAPPILGDTSKFEDSKYWLNADLSEFLSMRLSLVRGGVKYHKDDARLPDRFLLDIQSITISSRPVELDLRLKRIPSGGILDESLPPLGPSAPLEKLEIATLPPPLAVVEKVYVDNDMRAKDGIMKLYNAGIDVEKISRILSIGGIGKERKLVPTRWSITAVDKAISDTLIENIKNYDTIDKVEVYFRKYNKNLFIAILLPRDWSFEWGEAWYPGSTWNKFGNYVDIEVDNERYHGRSDYPEIGGCYYASRLGVTEFLASRKRQATAILWREIYEGFNLPVGVWFVRENVKEMFKGKPVVFDDISTALNFVKRLLRSDLKQWLSKSLLSFNTIDKWLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VC6</accession>
    <name>Q97VC6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DoxX family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2707</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90445">
        <property type="entry name" value="D90445"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988636.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VC6"/>
    <dbReference type="STRING" id="273057.SSO2707"/>
    <dbReference type="PaxDb" id="273057-SSO2707"/>
    <dbReference type="EnsemblBacteria" id="AAK42819">
        <property type="protein sequence ID" value="AAK42819"/>
        <property type="gene ID" value="SSO2707"/>
    </dbReference>
    <dbReference type="GeneID" id="1452738"/>
    <dbReference type="KEGG" id="sso:SSO2707"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2788"/>
    <dbReference type="eggNOG" id="arCOG08092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1902034_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VC6"/>
    <dbReference type="PhylomeDB" id="Q97VC6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051907">
        <property type="entry name" value="DoxX-like_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33452:SF1">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33452">
        <property type="entry name" value="OXIDOREDUCTASE CATD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07681">
        <property type="entry name" value="DoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="14117" checksum="0523D217B4B83E74" modified="2001-10-01" version="1">MDLTIASIGLLIFRILYGIALIPHGTAKANKNLNSQFKGYMRQLGVPPIFVDLSMLVEILGGLLVMIGAISLIVSAVLILFFLGTIVVSHRMKKPLATGMNPGMDLDILFLAGAIILLLLGPGDLAILPGPQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VD5</accession>
    <name>Q97VD5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Heat shock protein X, protease htpX homolog, putative (HtpX-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">htpX-like</name>
        <name evidence="13" type="ordered locus">SSO2694</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90444">
        <property type="entry name" value="B90444"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VD5"/>
    <dbReference type="FunCoup" id="Q97VD5">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2694"/>
    <dbReference type="PaxDb" id="273057-SSO2694"/>
    <dbReference type="EnsemblBacteria" id="AAK42809">
        <property type="protein sequence ID" value="AAK42809"/>
        <property type="gene ID" value="SSO2694"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2694"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2775"/>
    <dbReference type="eggNOG" id="arCOG01331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091224_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VD5"/>
    <dbReference type="PhylomeDB" id="Q97VD5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050083">
        <property type="entry name" value="HtpX_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221">
        <property type="entry name" value="PROTEASE HTPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221:SF2">
        <property type="entry name" value="PROTEASE HTPX HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 10 13" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0346">Stress response</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="12">
        <location>
            <begin position="83"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAK42809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="291" mass="34171" checksum="A8A82790C73D2861" modified="2001-10-01" version="1">MIILSFYKLLERVICVHSITTYWWKYYLASFFSILVIGIVISSLNINIPYFFLIQIGLVFSLWYLISPIIMILVFKMRRAPQDVTFMVEDLSKKYNVKLSKVYIINDNFLNAFAFGNIIFRGIAVTSPLYESLVQEELAAVLSHEIAHIKNYDPEVMLLTIFSINSIYAALIYYLPLVSLFFLIIYFVILLPSVFYVHRKIEKRADLTAVKVNPDITYWLESSLIKIGYLSRSIPTNMLRYVPPIQIFLAKEFIVNHIVDEKSVLSFRTHPSLKERLLYLSQYEKQKMYYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VG1</accession>
    <name>Q97VG1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">gluconate dehydratase</fullName>
            <ecNumber evidence="6">4.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2665</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18391"/>
            <dbReference type="ChEBI" id="CHEBI:57990"/>
            <dbReference type="EC" id="4.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the mandelate racemase/muconate lactonizing enzyme family. GaD subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.39" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90440">
        <property type="entry name" value="H90440"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="STRING" id="273057.SSO2665"/>
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    <dbReference type="EnsemblBacteria" id="AAK42783">
        <property type="protein sequence ID" value="AAK42783"/>
        <property type="gene ID" value="SSO2665"/>
    </dbReference>
    <dbReference type="GeneID" id="1452702"/>
    <dbReference type="KEGG" id="sso:SSO2665"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2747"/>
    <dbReference type="eggNOG" id="arCOG01168">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_030273_3_2_2"/>
    <dbReference type="InParanoid" id="Q97VG1"/>
    <dbReference type="PhylomeDB" id="Q97VG1"/>
    <dbReference type="BioCyc" id="MetaCyc:MONOMER-18070"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047929">
        <property type="term" value="F:gluconate dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03316">
        <property type="entry name" value="MR_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Putative L-rhamnonate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR034593">
        <property type="entry name" value="DgoD-like"/>
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        <property type="entry name" value="Enolase-like_C_sf"/>
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        <property type="entry name" value="Enolase-like_N"/>
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    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
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    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080:SF2">
        <property type="entry name" value="D-GALACTONATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080">
        <property type="entry name" value="D-GALACTONATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00179">
        <property type="entry name" value="mandelate_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00552">
        <property type="entry name" value="xylonate_dehydratase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mandelate racemase/muconate lactonizing enzyme C-terminal" evidence="7">
        <location>
            <begin position="139"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="393" mass="44761" checksum="3DA805B1507ACFC3" modified="2001-10-01" version="1">MTKISEIEAYILGKEVTSAQWASLMVLVRVTTNDGRVGWGETVSALRAEAVANFVKKINTVLKGNDVFNVEKNRLEWYKHDFNMTISLESTTAYSAVDIASWDIIGKELGAPLYKLLGGKTRDKVLVYANGWYQNCVKPEDFAEKAKEIVKMGYKALKFDPFGPYFNDISKKGLDIAEERVKAVREAVGDNVDILIEHHGRFNANSAIMIAKRLEKYNPLFMEEPIHPEDVEGLRKYRNNTSLRIALGERIINKQQALYFMKEGLVDFLQADLYRIGGVTETKKVVGIAETFDVQMAFHNAQGPILNAVTLQFDAFIPNFLIQESFYDWFPSWKRELIYNGTPIDNGYAIIPERPGLGVEVNEKMLDSLKVKGEEYFNPEEPVWVVKGTWRDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VH0</accession>
    <name>Q97VH0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Quinol oxidase (SoxABC), cytochrome B subunit (SoxC)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">soxC</name>
        <name evidence="13" type="ordered locus">SSO2656</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="G90439"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="Q97VH0">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2656"/>
    <dbReference type="PaxDb" id="273057-SSO2656"/>
    <dbReference type="EnsemblBacteria" id="AAK42774">
        <property type="protein sequence ID" value="AAK42774"/>
        <property type="gene ID" value="SSO2656"/>
    </dbReference>
    <dbReference type="GeneID" id="1452693"/>
    <dbReference type="GeneID" id="44128384"/>
    <dbReference type="KEGG" id="sso:SSO2656"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2738"/>
    <dbReference type="eggNOG" id="arCOG01721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038033_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VH0"/>
    <dbReference type="PhylomeDB" id="Q97VH0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.810.10:FF:000012">
        <property type="entry name" value="Cytochrome b/b6 domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.810.10">
        <property type="entry name" value="Cytochrome Bc1 Complex, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005798">
        <property type="entry name" value="Cyt_b/b6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036150">
        <property type="entry name" value="Cyt_b/b6_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005797">
        <property type="entry name" value="Cyt_b/b6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027387">
        <property type="entry name" value="Cytb/b6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053595">
        <property type="entry name" value="Cytochrome_b-type_ET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041076">
        <property type="entry name" value="cyt_b_SoxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19271">
        <property type="entry name" value="CYTOCHROME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19271:SF16">
        <property type="entry name" value="CYTOCHROME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00032">
        <property type="entry name" value="Cytochrom_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13631">
        <property type="entry name" value="Cytochrom_B_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81648">
        <property type="entry name" value="a domain/subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="Transmembrane di-heme cytochromes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51003">
        <property type="entry name" value="CYTB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51002">
        <property type="entry name" value="CYTB_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="321"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="389"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="455"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="500"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b/b6 N-terminal region profile" evidence="11">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b/b6 C-terminal region profile" evidence="12">
        <location>
            <begin position="230"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAK42774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="557" mass="62369" checksum="A0D26F9B6CA8B0F8" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VK4</accession>
    <name>Q97VK4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SpoVT-AbrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90435">
        <property type="entry name" value="E90435"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989344.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VK4"/>
    <dbReference type="STRING" id="273057.SSO2620"/>
    <dbReference type="PaxDb" id="273057-SSO2620"/>
    <dbReference type="EnsemblBacteria" id="AAK42740">
        <property type="protein sequence ID" value="AAK42740"/>
        <property type="gene ID" value="SSO2620"/>
    </dbReference>
    <dbReference type="GeneID" id="1454067"/>
    <dbReference type="KEGG" id="sso:SSO2620"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2702"/>
    <dbReference type="eggNOG" id="arCOG00320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_896058_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VK4"/>
    <dbReference type="PhylomeDB" id="Q97VK4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF5">
        <property type="entry name" value="PHOSPHATE UPTAKE REGULATOR, PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="11"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="315" mass="36412" checksum="4C28570A5C4AB175" modified="2001-10-01" version="1">MEKNLRRRIQKIKGGSYIITLPPEWVRRNNLDAKSEVFVIEKNGELLIKPIRDYITRKIIDLDLIDLETTKYLITVYYMQGISEIEIKSKSVISAPIKDELKNLQLYLPGLTIESESFNYITFKVQDDVNTNVVERMKTFSSKLLVLLKDLEKIIENPSKEMAIDLKNRAEELNKLYNSVIREIALVSQKEEEFVVKGLPTRDLILYAIAMRDMGRMLSHIKTASLAITECGNTSNDKELNSIIKIIVNMFEKSREVFFDENIDHVRSIRESMKEINKIVYGSKIECRELARELARIASYCVALMDDGVHKSVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VN1</accession>
    <name>Q97VN1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2588</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90432">
        <property type="entry name" value="A90432"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989296.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VN1"/>
    <dbReference type="FunCoup" id="Q97VN1">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2588"/>
    <dbReference type="PaxDb" id="273057-SSO2588"/>
    <dbReference type="EnsemblBacteria" id="AAK42712">
        <property type="protein sequence ID" value="AAK42712"/>
        <property type="gene ID" value="SSO2588"/>
    </dbReference>
    <dbReference type="GeneID" id="1454039"/>
    <dbReference type="KEGG" id="sso:SSO2588"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2669"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_12_1_2"/>
    <dbReference type="InParanoid" id="Q97VN1"/>
    <dbReference type="PhylomeDB" id="Q97VN1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09836">
        <property type="entry name" value="CBS_pair_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="9"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="72"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="142" mass="15582" checksum="BBE4DE3572664597" modified="2001-10-01" version="1">MAITSRSLIKRSPVVVKVGTKAIDACKIMYQNNIGSVVIVNEKDYPVGIFTERDVLRAVACGKDLNDKVENLGTFGKLVTVKSNSSIGEIAEKMVKNNIRHIVVVDDEGKLIGVVSIKDIVNEKHVLDFLVRSELNWEGGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VN3</accession>
    <name>Q97VN3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-methylcitrate dehydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2586</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PrpD family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90431">
        <property type="entry name" value="G90431"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989294.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VN3"/>
    <dbReference type="FunCoup" id="Q97VN3">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2586"/>
    <dbReference type="PaxDb" id="273057-SSO2586"/>
    <dbReference type="EnsemblBacteria" id="AAK42710">
        <property type="protein sequence ID" value="AAK42710"/>
        <property type="gene ID" value="SSO2586"/>
    </dbReference>
    <dbReference type="GeneID" id="1454037"/>
    <dbReference type="KEGG" id="sso:SSO2586"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2667"/>
    <dbReference type="eggNOG" id="arCOG04285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026574_3_0_2"/>
    <dbReference type="InParanoid" id="Q97VN3"/>
    <dbReference type="PhylomeDB" id="Q97VN3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4100.10">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.120">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036148">
        <property type="entry name" value="MmgE/PrpD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042183">
        <property type="entry name" value="MmgE/PrpD_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042188">
        <property type="entry name" value="MmgE/PrpD_sf_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005656">
        <property type="entry name" value="MmgE_PrpD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045337">
        <property type="entry name" value="MmgE_PrpD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045336">
        <property type="entry name" value="MmgE_PrpD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943:SF8">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19305">
        <property type="entry name" value="MmgE_PrpD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03972">
        <property type="entry name" value="MmgE_PrpD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103378">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MmgE/PrpD N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="MmgE/PrpD C-terminal" evidence="3">
        <location>
            <begin position="260"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="48672" checksum="DF3B629C9C636E92" modified="2001-10-01" version="1">MEIAEKIAEFVSSVNYQNLSEKVIHEAKRRIIDAIAVARGALNSPPHLVNKNVVTYFLGSVPLLFGGNATPDFAAFYNTFLIRYLDFNDTYLSKEPLHPSDMIGAFLSLGSLFNLEGKDVIEAVAIGYEVGVKLCDATSLRKKGFDHVTFLQVGAAAGLAKMLKLDEKSTVNAISLTLVPNIALRETRSGELSMWKAGAAADASRKAVFGALLAKFGMTSPSKPFSGRLGFVNVVAKDFDSTVFNRLEDDGILKTSLKKYPVEYHAEAVVEAGKSFNVNVDDIIKIEVETYEAAKTIIADEEKWNPTNKETADHSLPYILAYTLIKKDFWLDAYDKEMIFNEKIRNLMKKITVIEDEKYTSIYPKELPVKVTIYTTKGKDTIEVRNPRGYYNNPMTDAEVEEKYLRLKGRKEELNILWNMEELKVKEIVSSIKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VP9</accession>
    <name>Q97VP9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1359</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO10674</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90429">
        <property type="entry name" value="G90429"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VP9"/>
    <dbReference type="PaxDb" id="273057-SSO10674"/>
    <dbReference type="EnsemblBacteria" id="AAK42694">
        <property type="protein sequence ID" value="AAK42694"/>
        <property type="gene ID" value="SSO10674"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO10674"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2629897_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VP9"/>
    <dbReference type="PhylomeDB" id="Q97VP9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="77" mass="9272" checksum="4244A5D13FFE5EF0" modified="2001-10-01" version="1">MRKELVLVLLSYLLLNVWFLIRSWRKVKLWEFSVSLSNLLDREVRVEQGRAFREVKTSFPKLQLTLCTSFQLREINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VQ0</accession>
    <name>Q97VQ0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2566</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90429">
        <property type="entry name" value="E90429"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923925.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VQ0"/>
    <dbReference type="PaxDb" id="273057-SSO2566"/>
    <dbReference type="EnsemblBacteria" id="AAK42692">
        <property type="protein sequence ID" value="AAK42692"/>
        <property type="gene ID" value="SSO2566"/>
    </dbReference>
    <dbReference type="GeneID" id="1454019"/>
    <dbReference type="KEGG" id="sso:SSO2566"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2646"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077283_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VQ0"/>
    <dbReference type="PhylomeDB" id="Q97VQ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="354" mass="40264" checksum="75446031C8CBB1D0" modified="2001-10-01" version="1">MTKELTREEYYKALEKAVNEVILSMTGTRKDVAKRLLLGAVVGRNATEIAQEAEMDYETVLNNLDKAAQAKLIEVVKKLVGDHPVLLIVDDTHDHKLYARAMPVSRNGAQIFYCRAHKRFEPAIQLLVIGVKDLVNNQIYVIHIIAYIPRKVEEELKRRGEEVKFKTKIDALLEFLSSLSGLNVKSKVFDSWYVNSRTLQGNTVGELKSSARVVEGGRSVPVSEFPQGEYLVEYLGTPIKLLVIDDYKGYGRRYFFSTDLNDTAEDIITTWENRWDIEVLIRELKALGLEGGSFLTWVRNSGFVALKALSLLVVQYFKYSTGLMLGAKRLARLIKSIYREAGGIKKLFKRRRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VU7</accession>
    <name>Q97VU7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Medium-chain-fatty-acid--CoA ligase (AlkK-2)</fullName>
            <ecNumber evidence="7">6.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">alkK-2</name>
        <name evidence="7" type="ordered locus">SSO2510</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.-" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90423">
        <property type="entry name" value="D90423"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988492.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VU7"/>
    <dbReference type="STRING" id="273057.SSO2510"/>
    <dbReference type="PaxDb" id="273057-SSO2510"/>
    <dbReference type="EnsemblBacteria" id="AAK42643">
        <property type="protein sequence ID" value="AAK42643"/>
        <property type="gene ID" value="SSO2510"/>
    </dbReference>
    <dbReference type="GeneID" id="1453970"/>
    <dbReference type="KEGG" id="sso:SSO2510"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2589"/>
    <dbReference type="eggNOG" id="arCOG00856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_5_2"/>
    <dbReference type="InParanoid" id="Q97VU7"/>
    <dbReference type="PhylomeDB" id="Q97VU7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000008">
        <property type="entry name" value="2,3-dihydroxybenzoate-AMP ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004837">
        <property type="entry name" value="PRK06187.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859:SF4">
        <property type="entry name" value="BUTANOATE--COA LIGASE AAE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="43"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="458"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="552" mass="62391" checksum="1DD7D5CDCB2F5721" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W16</accession>
    <name>Q97W16_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carbon monoxide dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2431</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90414">
        <property type="entry name" value="G90414"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989392.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W16"/>
    <dbReference type="STRING" id="273057.SSO2431"/>
    <dbReference type="PaxDb" id="273057-SSO2431"/>
    <dbReference type="EnsemblBacteria" id="AAK42574">
        <property type="protein sequence ID" value="AAK42574"/>
        <property type="gene ID" value="SSO2431"/>
    </dbReference>
    <dbReference type="GeneID" id="1453901"/>
    <dbReference type="KEGG" id="sso:SSO2431"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2510"/>
    <dbReference type="eggNOG" id="arCOG01927">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046420_1_1_2"/>
    <dbReference type="InParanoid" id="Q97W16"/>
    <dbReference type="PhylomeDB" id="Q97W16"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05018">
        <property type="entry name" value="CoxG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010419">
        <property type="entry name" value="CO_DH_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38588">
        <property type="entry name" value="BLL0334 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38588:SF1">
        <property type="entry name" value="BLL0334 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06240">
        <property type="entry name" value="COXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15143" checksum="57512535289C3589" modified="2001-10-01" version="1">MKYEGSISINATKDQILKLLDNPDQVAQCFPGIKSFSKEGEEYKVVGVTGIGFIKGEYRANVRFEKIDDNKRKIIAKGTGMNSNVDIDAIAEALDGKINYSADVKVAGVLASVGARLMGSAVEKIINDLFNCIKERVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W79</accession>
    <name>Q97W79_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">succinate dehydrogenase</fullName>
            <ecNumber evidence="4">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">sdhA</name>
        <name evidence="14" type="ordered locus">SSO2356</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90406">
        <property type="entry name" value="F90406"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989496.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W79"/>
    <dbReference type="FunCoup" id="Q97W79">
        <property type="interactions" value="269"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2356"/>
    <dbReference type="PaxDb" id="273057-SSO2356"/>
    <dbReference type="EnsemblBacteria" id="AAK42509">
        <property type="protein sequence ID" value="AAK42509"/>
        <property type="gene ID" value="SSO2356"/>
    </dbReference>
    <dbReference type="GeneID" id="1453836"/>
    <dbReference type="KEGG" id="sso:SSO2356"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2443"/>
    <dbReference type="eggNOG" id="arCOG00571">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_6_2_2"/>
    <dbReference type="InParanoid" id="Q97W79"/>
    <dbReference type="PhylomeDB" id="Q97W79"/>
    <dbReference type="BRENDA" id="1.3.5.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000104">
        <property type="term" value="F:succinate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009061">
        <property type="term" value="P:anaerobic respiration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000003">
        <property type="entry name" value="Fumarate reductase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.40">
        <property type="entry name" value="succinate dehydrogenase protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005037">
        <property type="entry name" value="PRK06452.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="12">
        <location>
            <begin position="7"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="13">
        <location>
            <begin position="442"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000171-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAK42509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="566" mass="62532" checksum="C432C3B696BC397D" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W85</accession>
    <name>Q97W85_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO10258</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90405">
        <property type="entry name" value="H90405"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989503.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W85"/>
    <dbReference type="PaxDb" id="273057-SSO10258"/>
    <dbReference type="EnsemblBacteria" id="AAK42503">
        <property type="protein sequence ID" value="AAK42503"/>
        <property type="gene ID" value="SSO10258"/>
    </dbReference>
    <dbReference type="GeneID" id="1453830"/>
    <dbReference type="KEGG" id="sso:SSO10258"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2436"/>
    <dbReference type="eggNOG" id="arCOG07196">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197256_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W85"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="78" mass="9186" checksum="BF30EF919EE05481" modified="2001-10-01" version="1">MIVVVKYRIIDKNIRGIINSLRKIPFIKEILFYSGEKNSIFANGYMIWEEGSDLNPIEEVYDVKIIELSRRLYFPICG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-01-25" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W94</accession>
    <name>MTAP_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-methyl-5'-thioadenosine phosphorylase</fullName>
            <ecNumber evidence="1">2.4.2.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5'-methylthioadenosine phosphorylase</fullName>
            <shortName evidence="1">MTA phosphorylase</shortName>
            <shortName evidence="1">MTAP</shortName>
            <shortName evidence="1">MTAPII</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mtnP</name>
        <name type="ordered locus">SSO2343</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="FEBS J." volume="272" first="1886" last="1899">
            <title>A novel hyperthermostable 5'-deoxy-5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Cacciapuoti G."/>
                <person name="Forte S."/>
                <person name="Moretti M.A."/>
                <person name="Brio A."/>
                <person name="Zappia V."/>
                <person name="Porcelli M."/>
            </authorList>
            <dbReference type="PubMed" id="15819883"/>
            <dbReference type="DOI" id="10.1111/j.1742-4658.2005.04619.x"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>MUTAGENESIS OF CYS-259; CYS-261 AND CYS-262</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2006" name="J. Mol. Biol." volume="357" first="252" last="262">
            <title>The crystal structure of 5'-deoxy-5'-methylthioadenosine phosphorylase II from Sulfolobus solfataricus, a thermophilic enzyme stabilized by intramolecular disulfide bonds.</title>
            <authorList>
                <person name="Zhang Y."/>
                <person name="Porcelli M."/>
                <person name="Cacciapuoti G."/>
                <person name="Ealick S.E."/>
            </authorList>
            <dbReference type="PubMed" id="16414070"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.12.040"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) IN COMPLEX WITH SUBSTRATES</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-methyl-5'-thioadenosine + phosphate = 5-(methylsulfanyl)-alpha-D-ribose 1-phosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:11852"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.28"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">0.7 mM for S-methyl-5'-thioadenosine</KM>
            <KM evidence="2">270 uM for adenosine</KM>
        </kinetics>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 120 degrees Celsius. Highly thermostable.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3">Homohexamer. Dimer of a homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.28" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90404">
        <property type="entry name" value="G90404"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989516.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="PDB" id="2A8Y">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.45 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-270"/>
    </dbReference>
    <dbReference type="PDB" id="3T94">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.45 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-270"/>
    </dbReference>
    <dbReference type="PDBsum" id="2A8Y"/>
    <dbReference type="PDBsum" id="3T94"/>
    <dbReference type="AlphaFoldDB" id="Q97W94"/>
    <dbReference type="SMR" id="Q97W94"/>
    <dbReference type="FunCoup" id="Q97W94">
        <property type="interactions" value="265"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2343"/>
    <dbReference type="PaxDb" id="273057-SSO2343"/>
    <dbReference type="EnsemblBacteria" id="AAK42494">
        <property type="protein sequence ID" value="AAK42494"/>
        <property type="gene ID" value="SSO2343"/>
    </dbReference>
    <dbReference type="GeneID" id="1453821"/>
    <dbReference type="KEGG" id="sso:SSO2343"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2427"/>
    <dbReference type="eggNOG" id="arCOG01327">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054456_0_2_2"/>
    <dbReference type="InParanoid" id="Q97W94"/>
    <dbReference type="PhylomeDB" id="Q97W94"/>
    <dbReference type="BRENDA" id="2.4.2.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="2.4.2.28">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00873"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97W94"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000012">
        <property type="entry name" value="Probable 6-oxopurine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018099">
        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01694">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006334">
        <property type="entry name" value="PRK08564.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679:SF3">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0660">Purine salvage</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000415109" description="S-methyl-5'-thioadenosine phosphorylase">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="58"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="91"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="214"/>
            <end position="216"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="138"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="200"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="259"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces thermostability of the enzyme; when associated with S-261." evidence="2">
        <original>C</original>
        <variation>S</variation>
        <location>
            <position position="259"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces thermostability of the enzyme; when associated with S-259." evidence="2">
        <original>C</original>
        <variation>S</variation>
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces thermostability of the enzyme." evidence="2">
        <original>C</original>
        <variation>S</variation>
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="9"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="25"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="51"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="58"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="61"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="67"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="110"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="125"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="156"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="161"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="173"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="187"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="218"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="236"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="256"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="260"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="266"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15819883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="16414070"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2A8Y"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="3T94"/>
        </source>
    </evidence>
    <sequence length="270" mass="30141" checksum="682A166698935A88" modified="2001-10-01" version="1">MIEQNEKASIGIIGGSGLYDPGIFSESKEIKVYTPYGQPSDFITIGKIGNKSVAFLPRHGRGHRIPPHKINYRANIWALKELGVRWVISVSAVGSLRMDYKLGDFVIPDQFIDMTKNREYSFFDGPVVAHVSMADPFCNSLRKLAIETAKELNIKTHESGTYICIEGPRFSTRAESRTWREVYKADIIGMTLVPEVNLACEAQMCYATIAMVTDYDVFAEIPVTAEEVTRVMAENTEKAKKLLYALIQKLPEKPEEGSCSCCNSLKTALV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-06-21" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WG9</accession>
    <name>MRE11_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA double-strand break repair protein Mre11</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mre11</name>
        <name type="synonym">rad32</name>
        <name type="ordered locus">SSO2250</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Mre11 binds to DSB ends and has both double-stranded 3'-5' exonuclease activity and single-stranded endonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Nuclease activity is regulated by Rad50.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MRE11/RAD32 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90395">
        <property type="entry name" value="C90395"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991541.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WG9"/>
    <dbReference type="SMR" id="Q97WG9"/>
    <dbReference type="STRING" id="273057.SSO2250"/>
    <dbReference type="PaxDb" id="273057-SSO2250"/>
    <dbReference type="EnsemblBacteria" id="AAK42418">
        <property type="protein sequence ID" value="AAK42418"/>
        <property type="gene ID" value="SSO2250"/>
    </dbReference>
    <dbReference type="GeneID" id="1453745"/>
    <dbReference type="GeneID" id="44127984"/>
    <dbReference type="KEGG" id="sso:SSO2250"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2345"/>
    <dbReference type="eggNOG" id="arCOG00397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026621_5_2_2"/>
    <dbReference type="InParanoid" id="Q97WG9"/>
    <dbReference type="PhylomeDB" id="Q97WG9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045027">
        <property type="term" value="F:DNA end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004529">
        <property type="term" value="F:DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000129">
        <property type="entry name" value="DNA double-strand break repair protein Mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02044">
        <property type="entry name" value="Mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050535">
        <property type="entry name" value="DNA_Repair-Maintenance_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053459">
        <property type="entry name" value="DSB_Repair_Mre11/Rad50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032885">
        <property type="entry name" value="Mre11_archaea-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041031">
        <property type="entry name" value="Mre11_Sulfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337">
        <property type="entry name" value="COMPONENT OF ATP-DEPENDENT DSDNA EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337:SF0">
        <property type="entry name" value="NUCLEASE SBCCD SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000138699" description="DNA double-strand break repair protein Mre11">
        <location>
            <begin position="1"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02044"/>
        </source>
    </evidence>
    <sequence length="381" mass="43551" checksum="D44BDEAD2C100440" modified="2001-10-01" version="1">MVQILHISDTHLGKRQYSLVEREKDIYDIFSQLVDIAIKEHVDVIIHSGDLFDVSSPTTNALVMAIKILKRLKDVNIPFLSIPGDHDTPKRKGYLIPHNILSELDLIKILNYEKPYIIKGIEVYGIPHIPTVSKSILVSALSALRPKSSRSILLLHQGVKQILPYDGSWQMELGSLPKGFGYYALGHIHTRWRLTQDDGSVIAIAGSPDIMREEEIEGYEKFGKGAYLIDFSKDLPILSTINITVRPQKVVTINTKNIKKDILTIRDDLIEHNKSENNKPILHIIVEGERMRKDLLYKELLPLNDVALYYRIYKDETTQTVDNLSYTLPRDKGLDKIIIEYLTKYEKFSEDEANLILQMIKNVESDEIVNEILKKLTGVNL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-10" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WJ0</accession>
    <name>NEP1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA small subunit methyltransferase Nep1</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">16S rRNA (pseudouridine-N1-)-methyltransferase Nep1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nep1</name>
        <name type="ordered locus">SSO2226</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Methyltransferase involved in ribosomal biogenesis. Specifically catalyzes the N1-methylation of the pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Fully modified pseudouridine 930 is seen in 16S rRNA in ribosomal 30S initiation complexes (PubMed:39753558).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>a pseudouridine in rRNA + S-adenosyl-L-methionine = an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46696"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:13933"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK42396" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42396.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90392">
        <property type="entry name" value="E90392"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WJ0"/>
    <dbReference type="SMR" id="Q97WJ0"/>
    <dbReference type="FunCoup" id="Q97WJ0">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2226"/>
    <dbReference type="PaxDb" id="273057-SSO2226"/>
    <dbReference type="EnsemblBacteria" id="AAK42396">
        <property type="protein sequence ID" value="AAK42396"/>
        <property type="gene ID" value="SSO2226"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2226"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2322"/>
    <dbReference type="eggNOG" id="arCOG04122">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055846_1_3_2"/>
    <dbReference type="InParanoid" id="Q97WJ0"/>
    <dbReference type="PhylomeDB" id="Q97WJ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070037">
        <property type="term" value="F:rRNA (pseudouridine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18088">
        <property type="entry name" value="Nep1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000042">
        <property type="entry name" value="Ribosomal RNA small subunit methyltransferase Nep1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00554">
        <property type="entry name" value="NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005304">
        <property type="entry name" value="Rbsml_bgen_MeTrfase_EMG1/NEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023503">
        <property type="entry name" value="Ribosome_NEP1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003203">
        <property type="entry name" value="PRK04171.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636">
        <property type="entry name" value="NEP1/MRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636:SF5">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03587">
        <property type="entry name" value="EMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000158622" description="Ribosomal RNA small subunit methyltransferase Nep1">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="193"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="1">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="site" description="Stabilizes Arg-61" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="1">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="1">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="1">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <sequence length="218" mass="24933" checksum="8DD248A26FECFF49" modified="2002-05-10" version="2">MHLNIILLEASLELVPKEIVNHPAVIKNAKRRNKKPEDTLLDISLHYHAMKYLENSHKRGRPDILHQALLVILTDPVIKGDIFIHTIQSKIIKVNPNMRPPKNYLRFIGLMEQLLKYGKIPINGDETLMEVTNLTLDNIVNRYDLILLSEKGEKINPEEICKLDEKWLLGIGAFPHGDFSDKILNLAKKIYSISGFPLETQQVLCRIFSACNSILGWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WJ7</accession>
    <name>Q97WJ7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2220</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90391">
        <property type="entry name" value="F90391"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992062.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WJ7"/>
    <dbReference type="STRING" id="273057.SSO2220"/>
    <dbReference type="PaxDb" id="273057-SSO2220"/>
    <dbReference type="EnsemblBacteria" id="AAK42389">
        <property type="protein sequence ID" value="AAK42389"/>
        <property type="gene ID" value="SSO2220"/>
    </dbReference>
    <dbReference type="GeneID" id="1453716"/>
    <dbReference type="KEGG" id="sso:SSO2220"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2316"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059688_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WJ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050375">
        <property type="entry name" value="MFS_TsgA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43702">
        <property type="entry name" value="L-FUCOSE-PROTON SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43702:SF3">
        <property type="entry name" value="PROTEIN TSGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="1"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="355" mass="39030" checksum="811760466D892719" modified="2001-10-01" version="1">MKGIKIIVARVIYAIHWFYLAPLIPFLAGKLNLSLQIVGLIPLSFFLGSGIMQIPSAFLSTRIGLRNTLLLGLFIMSISPLLIPLSSNFIELLIFYLMDGVGASMFFSTGGGILASLNRDSPSLALGLYNASFAVGGLIGLNWYIVFGNYLSFYILSILTFLSLIINVNNSNLKPCWRIIRDNRIVILGISIAGIWGVYYVIGELYPTFAYYYLHLNLIDSSLFTSLLLVSSVIGGSLTFLADRKIKKFNLLIISSLLGSVPSLLLYTKVYLLGIVITGIFNELAISVLYSIIASLQRDANATMGLALVNSLNILIGMNFELLASYSGYYMWIAVNIIGLLLFSLIVLVRQNISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WL0</accession>
    <name>Q97WL0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional activator (TenA-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tenA-1</name>
        <name evidence="2" type="ordered locus">SSO2206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2007-08" db="PDB data bank">
            <title>Crystal structure of putative TenA-like thiaminase II (NP_343586.1) from Sulfolobus solfataricus at 1.50 A resolution.</title>
            <authorList>
                <consortium name="Joint Center for Structural Genomics (JCSG)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99390">
        <property type="entry name" value="A99390"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992080.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2QZC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.50 A"/>
        <property type="chains" value="A/B=1-213"/>
    </dbReference>
    <dbReference type="PDBsum" id="2QZC"/>
    <dbReference type="AlphaFoldDB" id="Q97WL0"/>
    <dbReference type="SMR" id="Q97WL0"/>
    <dbReference type="FunCoup" id="Q97WL0">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2206"/>
    <dbReference type="PaxDb" id="273057-SSO2206"/>
    <dbReference type="DNASU" id="1453703"/>
    <dbReference type="EnsemblBacteria" id="AAK42376">
        <property type="protein sequence ID" value="AAK42376"/>
        <property type="gene ID" value="SSO2206"/>
    </dbReference>
    <dbReference type="GeneID" id="1453703"/>
    <dbReference type="KEGG" id="sso:SSO2206"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2302"/>
    <dbReference type="eggNOG" id="arCOG01128">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077537_3_0_2"/>
    <dbReference type="InParanoid" id="Q97WL0"/>
    <dbReference type="PhylomeDB" id="Q97WL0"/>
    <dbReference type="EvolutionaryTrace" id="Q97WL0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19362">
        <property type="entry name" value="TenA_C_SsTenA-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.910.10:FF:000027">
        <property type="entry name" value="Transcriptional activator, TenA family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050967">
        <property type="entry name" value="Thiamine_Salvage_TenA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198">
        <property type="entry name" value="BIFUNCTIONAL TH2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198:SF2">
        <property type="entry name" value="SI:CH1073-67J19.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiaminase-2/PQQC" evidence="1">
        <location>
            <begin position="16"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2QZC"/>
        </source>
    </evidence>
    <sequence length="213" mass="24787" checksum="788EF039211351CD" modified="2001-10-01" version="1">MSIVGNVENLINGVGELWNKYVKHEFILKMRDGSLPLDIFRYYLIQDGKYVEDMLRALLIASSKGPIDKVTKILNLVFSSRDKGLETHGKLYSKLDISRDVIVKTGYNLINYAYTRHLYYYANLDWNKFLVAWTPCMFGYSIVGDYVIDSPNEVYKTWASFYASTEYKKRIEAILYALDEVSITEDLLNIFINSVRFEIGFWDASLRKDPTVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WN8</accession>
    <name>Q97WN8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Amidohydrolase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2174</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90386">
        <property type="entry name" value="E90386"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992130.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WN8"/>
    <dbReference type="FunCoup" id="Q97WN8">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2174"/>
    <dbReference type="PaxDb" id="273057-SSO2174"/>
    <dbReference type="EnsemblBacteria" id="AAK42348">
        <property type="protein sequence ID" value="AAK42348"/>
        <property type="gene ID" value="SSO2174"/>
    </dbReference>
    <dbReference type="GeneID" id="1453675"/>
    <dbReference type="KEGG" id="sso:SSO2174"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2268"/>
    <dbReference type="eggNOG" id="arCOG00062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_3_6_2"/>
    <dbReference type="InParanoid" id="Q97WN8"/>
    <dbReference type="PhylomeDB" id="Q97WN8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07586">
        <property type="entry name" value="nitrilase_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050345">
        <property type="entry name" value="Aliph_Amidase/BUP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674:SF2">
        <property type="entry name" value="BETA-UREIDOPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674">
        <property type="entry name" value="NITRILASE C965.09-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="270" mass="31320" checksum="8AEEF63528EB6178" modified="2001-10-01" version="1">MGIKVELAQIRSYPGDVYRNYKKHLEIIESSSADCVIFPELSLTGYIIKDLTYEIYKDAEEATQKIAEKVNKCVVFGTIRQVRKGILRNSAAVIINGKLDYIYKFYLPTYGLFEERRYFQRGDPLKDLKIFEYKDLKFGVVICEDAWHPEPIEALSLMGADAIFIPSASPMRKLRENLAIEESWDSLLKAHSLMNTVWTVFTNVVGSQEEEYFWGGSRVVSPLGDVKLKLKLFYEDRGTIEITENELLRARFFSSYRDHIREFHSILDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WY3</accession>
    <name>Q97WY3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1977</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90364">
        <property type="entry name" value="A90364"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989371.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WY3"/>
    <dbReference type="PaxDb" id="273057-SSO1977"/>
    <dbReference type="EnsemblBacteria" id="AAK42168">
        <property type="protein sequence ID" value="AAK42168"/>
        <property type="gene ID" value="SSO1977"/>
    </dbReference>
    <dbReference type="GeneID" id="1453495"/>
    <dbReference type="KEGG" id="sso:SSO1977"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2054"/>
    <dbReference type="HOGENOM" id="CLU_129118_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WY3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="132" mass="14953" checksum="C650ACF7653694C3" modified="2001-10-01" version="1">MDNVNVTLTPHLVSYVFNVYIKYELPCGKVCCLYYCTIGKYYSPGSIINISFTAYRYSQNLELIGVEPSTYFTLISHGQTEAHCCLKSWIAVKLNNVSVYTSIYIVVKAMSPSSATPKFNIPMLPIIDRRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X81</accession>
    <name>Q97X81_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF929 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90350">
        <property type="entry name" value="F90350"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923634.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X81"/>
    <dbReference type="STRING" id="273057.SSO1873"/>
    <dbReference type="PaxDb" id="273057-SSO1873"/>
    <dbReference type="EnsemblBacteria" id="AAK42061">
        <property type="protein sequence ID" value="AAK42061"/>
        <property type="gene ID" value="SSO1873"/>
    </dbReference>
    <dbReference type="GeneID" id="1453388"/>
    <dbReference type="KEGG" id="sso:SSO1873"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1924"/>
    <dbReference type="eggNOG" id="arCOG03842">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077328_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X81"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009272">
        <property type="entry name" value="DUF929"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06053">
        <property type="entry name" value="DUF929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="30563" checksum="99CBA54724D11B72" modified="2001-10-01" version="1">MRRQTSRYLLITIVIIAIIVIAGSALSQLRSTSSPLIDKPIAGDVYNQLVEISNQGYNITALPTNDFKVLPLNFSSNGKPAIIYVGAEWCPYCGAERWALIIALLRFGNFSNLEYMVSSPTDVYPNTPTFTFANSTYNSPYISFYGIEYQDRNYQPLDKVPTPIYNMWENYGNLSIPFLIIGYYYKVGTSIDPGLLSGHNWTYVLDQLHNPNSLVYKEIYSEANLITQMICKVDGYKPYNVCSHFMQASVNSDSSLFYVSTSLRINSGDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XB4</accession>
    <name>Q97XB4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1831</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90346">
        <property type="entry name" value="D90346"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992461.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XB4"/>
    <dbReference type="STRING" id="273057.SSO1831"/>
    <dbReference type="PaxDb" id="273057-SSO1831"/>
    <dbReference type="EnsemblBacteria" id="AAK42027">
        <property type="protein sequence ID" value="AAK42027"/>
        <property type="gene ID" value="SSO1831"/>
    </dbReference>
    <dbReference type="GeneID" id="1453354"/>
    <dbReference type="KEGG" id="sso:SSO1831"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1891"/>
    <dbReference type="eggNOG" id="arCOG07310">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1648337_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XB4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="172" mass="19845" checksum="14A06A4E90478013" modified="2001-10-01" version="1">MPIIVRKVHKKDDGSTVWIRIGESPPVIKEGNVIDGGFFIRIGDDKSDKIIRLSDQEALDIAYRIIEAYRRHVRIYAKLDELSYEQYKRSKELAKDEDEEDIEKIEEKKNLTPDKIEAELINYLITAGGETTLDKVREDLGEEYAAYLKTMKNKGIIIEGIRVKLGKDFKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XF1</accession>
    <name>Q97XF1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial transposase in ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1798</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90341">
        <property type="entry name" value="G90341"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XF1"/>
    <dbReference type="STRING" id="273057.SSO1798"/>
    <dbReference type="PaxDb" id="273057-SSO1798"/>
    <dbReference type="EnsemblBacteria" id="AAK41990">
        <property type="protein sequence ID" value="AAK41990"/>
        <property type="gene ID" value="SSO1798"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1798"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2230505_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XF1"/>
    <dbReference type="PhylomeDB" id="Q97XF1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="105" mass="12533" checksum="69F98003E41E0097" modified="2001-10-01" version="1">MKLILYIYILLLKTDLLTVIFIKYMQLYLNKTGAVQSYIYYHLYQKNEIPILKSNIIVIEPTGVYSTRPCQYFKERGVKVLLVSLAMERKGHKGKENRFLRRRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XG2</accession>
    <name>Q97XG2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Polysaccharide biosynthesis related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1780</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90339">
        <property type="entry name" value="E90339"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923582.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XG2"/>
    <dbReference type="STRING" id="273057.SSO1780"/>
    <dbReference type="PaxDb" id="273057-SSO1780"/>
    <dbReference type="EnsemblBacteria" id="AAK41972">
        <property type="protein sequence ID" value="AAK41972"/>
        <property type="gene ID" value="SSO1780"/>
    </dbReference>
    <dbReference type="GeneID" id="1454751"/>
    <dbReference type="KEGG" id="sso:SSO1780"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1830"/>
    <dbReference type="eggNOG" id="arCOG02209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046380_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XG2"/>
    <dbReference type="PhylomeDB" id="Q97XG2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052556">
        <property type="entry name" value="PolySynth_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43424">
        <property type="entry name" value="LOCUS PUTATIVE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43424:SF1">
        <property type="entry name" value="LOCUS PUTATIVE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="313"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="403"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="424"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="468" mass="52141" checksum="81655EA954589DF6" modified="2001-10-01" version="1">MNPIKNALKNLSVTTTNVIIALIFFVITAKISSPAFFGKVAIVQLLEIVTSTFFYFIPGQIITREISYLYAKKEITKEVVGKFLSFPFLVLPIFLILLIFPDYVKLAIPYLFLYLLNGVMTAVMIGMDMFTESAITGNFFLVIRWGIAIIAVLYHNIYLFVKIWTLGGILSVSMNYAFISKKVGLVLPTPDFAFLFRHFREGLPVYLSSFAGFLSSQGDRVTTAYLLGSYYLGIYQFSALVAGVPSMILGALGGVLLPTASFYKALGKDEKKMSSLSFIFLSLLTFLTVIISIPIGEIIIIHFFPNYKEGLEVFVLLLISATLPFPIGSLTNFIVAFKRNLRPFLILSILNGSLVLLTSYLLIPRIGIMGGAISQVIVATISSLFIIFYSIRTSVFSTGRKEIILLFLIPVVGIYEAIDPPFLDFLLILLILLVFKLFKIITEEDVKIIEGFLPHGLKFVSKILSKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y02</accession>
    <name>Q97Y02_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1395</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8351</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90314">
        <property type="entry name" value="B90314"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y02"/>
    <dbReference type="STRING" id="273057.SSO8351"/>
    <dbReference type="PaxDb" id="273057-SSO8351"/>
    <dbReference type="EnsemblBacteria" id="AAK41769">
        <property type="protein sequence ID" value="AAK41769"/>
        <property type="gene ID" value="SSO8351"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8351"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1591"/>
    <dbReference type="eggNOG" id="arCOG02127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2597916_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y02"/>
    <dbReference type="PhylomeDB" id="Q97Y02"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13565">
        <property type="entry name" value="HTH_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="79" mass="9741" checksum="DDDCF953C83D43D4" modified="2001-10-01" version="1">MSTVYRWINQFEKEGEKCLFYKERKGRNKKIDEKEIKVEELQGKTIWEAKAYIEEKFHVKLSYVTAWRIARKRLKIPYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y04</accession>
    <name>Q97Y04_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1552</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90313">
        <property type="entry name" value="H90313"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y04"/>
    <dbReference type="SMR" id="Q97Y04"/>
    <dbReference type="STRING" id="273057.SSO1552"/>
    <dbReference type="PaxDb" id="273057-SSO1552"/>
    <dbReference type="EnsemblBacteria" id="AAK41767">
        <property type="protein sequence ID" value="AAK41767"/>
        <property type="gene ID" value="SSO1552"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1552"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1587"/>
    <dbReference type="eggNOG" id="arCOG00945">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_3_1_2"/>
    <dbReference type="InParanoid" id="Q97Y04"/>
    <dbReference type="PhylomeDB" id="Q97Y04"/>
    <dbReference type="UniPathway" id="UPA00782"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787:SF3">
        <property type="entry name" value="ARYLSULFATASE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787">
        <property type="entry name" value="FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="8"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="342" mass="39650" checksum="16F43CA8C926BEA2" modified="2001-10-01" version="1">MSGMIGKIQDKKIFSITIIPSSWCNFRCVYCYENDHYKIKNMITTSEMTKRIKLFLENNIKTGFFRKVHIRWYGGEPLLDFKTIIDIMDFTNSIGKTYKTDIIGEIITNGYLLDIVKFKILLEKGVKIFQVTIDGYKDAHDKLRPLLNGKPTFDKIFENLIQTRNVTDNFFMFIRSNVMPDNLDSIKKFLDLASNYFSRDRRYYFTFFPVTRSGGDNDKNLKVIEPKKRMEIIQELYMYATQLGLNIPDKDTLLQIDGVPAMDDLCQALYPFSIIIRNDGKLFKCPLTVMIDDESVGHIEEDGSIVLDESKINRWAKYILSPLSRGCPRLHELGEVVGVEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y14</accession>
    <name>Q97Y14_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH bat-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1536</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90312">
        <property type="entry name" value="F90312"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923458.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y14"/>
    <dbReference type="PaxDb" id="273057-SSO1536"/>
    <dbReference type="EnsemblBacteria" id="AAK41757">
        <property type="protein sequence ID" value="AAK41757"/>
        <property type="gene ID" value="SSO1536"/>
    </dbReference>
    <dbReference type="GeneID" id="1454536"/>
    <dbReference type="KEGG" id="sso:SSO1536"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1574"/>
    <dbReference type="eggNOG" id="arCOG02272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104910_1_0_2"/>
    <dbReference type="InParanoid" id="Q97Y14"/>
    <dbReference type="PhylomeDB" id="Q97Y14"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH bat-type" evidence="2">
        <location>
            <begin position="153"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="215" mass="25130" checksum="ED4C436AF70A0282" modified="2001-10-01" version="1">MEKLSLISADLVHPDCWTERTEKYLVNVKLLSQQFSDDKFFVSKVLILGKDSRNLIDELKNFKGVKINKLYCLDEDSFVMDFIYPRYNSVSSLFYDTNSIVISHRIVDGIEKWKIIASSSMVSHILEKLENTTNLIDFKEINLKKLERLLDKLTDRNLSFLKIAHKQGLFDYPKRKTLLSLSKELGIKPNTLLYHIRKSESSLLEILIDEYYSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y64</accession>
    <name>Q97Y64_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Second ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1480</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90306">
        <property type="entry name" value="B90306"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y64"/>
    <dbReference type="STRING" id="273057.SSO1480"/>
    <dbReference type="PaxDb" id="273057-SSO1480"/>
    <dbReference type="EnsemblBacteria" id="AAK41705">
        <property type="protein sequence ID" value="AAK41705"/>
        <property type="gene ID" value="SSO1480"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1480"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1518"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074024_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y64"/>
    <dbReference type="PhylomeDB" id="Q97Y64"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033541">
        <property type="entry name" value="transpos_ISH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="41"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="297" mass="34614" checksum="367E9E6FA8C8920A" modified="2001-10-01" version="1">MSPQTVRNYVEEQGTEVIEKLLESMRRISMEILKGVKEVDISIDWTTKTWYGKPVEGLGSSAKGNSWNYATAITKYQNMVLLLAFVPQVNGMSKDEIVKLLMEQIVGMGLKVGLVTLDAGFYTVEVLKFISQFKFVIGVPVGDVKIYEEFDGEYTTNSKRYKKEEQVKFRLLVYGKEIVKKRKKTVVYFARATNLDLPKREVLKLYNKVRSPIETSYRNIKAFLPFTSSTKFIFRELIFVLAMIFYSLYTVFKNVMTREEFRLLLILCFLDDLSDLKDFIFNLEETLINTIDLFLWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y79</accession>
    <name>Q97Y79_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Penicillin acylase</fullName>
            <ecNumber evidence="4">3.5.1.11</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1460</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S45 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.11" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99304">
        <property type="entry name" value="A99304"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923415.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y79"/>
    <dbReference type="STRING" id="273057.SSO1460"/>
    <dbReference type="MEROPS" id="S45.003"/>
    <dbReference type="PaxDb" id="273057-SSO1460"/>
    <dbReference type="EnsemblBacteria" id="AAK41688">
        <property type="protein sequence ID" value="AAK41688"/>
        <property type="gene ID" value="SSO1460"/>
    </dbReference>
    <dbReference type="GeneID" id="1454467"/>
    <dbReference type="KEGG" id="sso:SSO1460"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1492"/>
    <dbReference type="eggNOG" id="arCOG04082">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011790_4_0_2"/>
    <dbReference type="InParanoid" id="Q97Y79"/>
    <dbReference type="PhylomeDB" id="Q97Y79"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008953">
        <property type="term" value="F:penicillin amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017000">
        <property type="term" value="P:antibiotic biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.439.10">
        <property type="entry name" value="Penicillin Amidohydrolase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014395">
        <property type="entry name" value="Pen/GL7ACA/AHL_acylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043147">
        <property type="entry name" value="Penicillin_amidase_A-knob"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023343">
        <property type="entry name" value="Penicillin_amidase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043146">
        <property type="entry name" value="Penicillin_amidase_N_B-knob"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002692">
        <property type="entry name" value="S45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34218:SF4">
        <property type="entry name" value="ACYL-HOMOSERINE LACTONE ACYLASE QUIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34218">
        <property type="entry name" value="PEPTIDASE S45 PENICILLIN AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01804">
        <property type="entry name" value="Penicil_amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001227">
        <property type="entry name" value="Pen_acylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="831" mass="93459" checksum="A0D5A508F26A6D1A" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y85</accession>
    <name>CAS2B_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated endoribonuclease Cas2 2</fullName>
            <ecNumber>3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cas22</name>
        <name type="ordered locus">SSO8090</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="J. Biol. Chem." volume="283" first="20361" last="20371">
            <title>A novel family of sequence-specific endoribonucleases associated with the clustered regularly interspaced short palindromic repeats.</title>
            <authorList>
                <person name="Beloglazova N."/>
                <person name="Brown G."/>
                <person name="Zimmerman M.D."/>
                <person name="Proudfoot M."/>
                <person name="Makarova K.S."/>
                <person name="Kudritska M."/>
                <person name="Kochinyan S."/>
                <person name="Wang S."/>
                <person name="Chruszcz M."/>
                <person name="Minor W."/>
                <person name="Koonin E.V."/>
                <person name="Edwards A.M."/>
                <person name="Savchenko A."/>
                <person name="Yakunin A.F."/>
            </authorList>
            <dbReference type="PubMed" id="18482976"/>
            <dbReference type="DOI" id="10.1074/jbc.M803225200"/>
        </citation>
        <scope>FUNCTION AS A SSRNA-SPECIFIC ENDORIBONUCLEASE</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="submission" date="2008-10" db="PDB data bank">
            <title>Structure of the RNase SSO8090 from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Proudfoot M."/>
                <person name="Brown M."/>
                <person name="Singer A.U."/>
                <person name="Skarina T."/>
                <person name="Tan K."/>
                <person name="Kagan O."/>
                <person name="Edwards A.M."/>
                <person name="Joachimiak A."/>
                <person name="Savchenko A."/>
                <person name="Yakunin A.F."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer, forms a heterotetramer with a Cas1 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CRISPR-associated endoribonuclease Cas2 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK54438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990820.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3EXC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.25 A"/>
        <property type="chains" value="X=1-88"/>
    </dbReference>
    <dbReference type="PDBsum" id="3EXC"/>
    <dbReference type="AlphaFoldDB" id="Q97Y85"/>
    <dbReference type="SMR" id="Q97Y85"/>
    <dbReference type="STRING" id="273057.SSO8090"/>
    <dbReference type="PaxDb" id="273057-SSO8090"/>
    <dbReference type="EnsemblBacteria" id="AAK54438">
        <property type="protein sequence ID" value="AAK54438"/>
        <property type="gene ID" value="SSO8090"/>
    </dbReference>
    <dbReference type="GeneID" id="24780239"/>
    <dbReference type="KEGG" id="sso:SSO8090"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1479"/>
    <dbReference type="eggNOG" id="arCOG04194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161124_2_0_2"/>
    <dbReference type="InParanoid" id="Q97Y85"/>
    <dbReference type="PhylomeDB" id="Q97Y85"/>
    <dbReference type="EvolutionaryTrace" id="Q97Y85"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09725">
        <property type="entry name" value="Cas2_I_II_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01471">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021127">
        <property type="entry name" value="CRISPR_associated_Cas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019199">
        <property type="entry name" value="Virulence_VapD/CRISPR_Cas2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01573">
        <property type="entry name" value="cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405:SF3">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS2 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09827">
        <property type="entry name" value="CRISPR_Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143430">
        <property type="entry name" value="TTP0101/SSO1404-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000416952" description="CRISPR-associated endoribonuclease Cas2 2">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="2"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="28"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="34"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="57"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="61"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="69"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="18482976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="3EXC"/>
        </source>
    </evidence>
    <sequence length="88" mass="10224" checksum="870148D1138A7930" modified="2001-10-01" version="1">MKLLVVYDVSDDSKRNKLANNLKKLGLERIQRSAFEGDIDSQRVKDLVRVVKLIVDTNTDIVHIIPLGIRDWERRIVIGREGLEEWLV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y90</accession>
    <name>CSA5B_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR type I-A cluster 2/Apern-associated protein Csa5-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Probable CRISPR toxin Csa5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">csa5</name>
        <name type="ordered locus">SSO1443</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="J. Biol. Chem." volume="286" first="21643" last="21656">
            <title>Structural and functional characterization of an archaeal clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for antiviral defense (CASCADE).</title>
            <authorList>
                <person name="Lintner N.G."/>
                <person name="Kerou M."/>
                <person name="Brumfield S.K."/>
                <person name="Graham S."/>
                <person name="Liu H."/>
                <person name="Naismith J.H."/>
                <person name="Sdano M."/>
                <person name="Peng N."/>
                <person name="She Q."/>
                <person name="Copie V."/>
                <person name="Young M.J."/>
                <person name="White M.F."/>
                <person name="Lawrence C.M."/>
            </authorList>
            <dbReference type="PubMed" id="21507944"/>
            <dbReference type="DOI" id="10.1074/jbc.M111.238485"/>
        </citation>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35091 / DSM 1616 / JCM 8930 / NBRC 15331 / P1</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2013" name="RNA Biol." volume="10" first="762" last="769">
            <title>Structure of the archaeal Cascade subunit Csa5: relating the small subunits of CRISPR effector complexes.</title>
            <authorList>
                <person name="Reeks J."/>
                <person name="Graham S."/>
                <person name="Anderson L."/>
                <person name="Liu H."/>
                <person name="White M.F."/>
                <person name="Naismith J.H."/>
            </authorList>
            <dbReference type="PubMed" id="23846216"/>
            <dbReference type="DOI" id="10.4161/RNA.23854"/>
        </citation>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF ASP-32</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2014" name="J. Mol. Biol." volume="426" first="3683" last="3688">
            <title>First experimental evidence for the presence of a CRISPR toxin in sulfolobus.</title>
            <authorList>
                <person name="He F."/>
                <person name="Chen L."/>
                <person name="Peng X."/>
            </authorList>
            <dbReference type="PubMed" id="25277654"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2014.09.016"/>
        </citation>
        <scope>FUNCTION AS A TOXIN</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF ASP-32</scope>
        <source>
            <strain>P2 / 5E6</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="function">
        <text evidence="3">In strain P2 toxic when expressed in vivo in the absence of CRISPR loci A-D (strain P2 contains 6 CRISPR loci in all). It causes strong growth inhibition, a significant proportion of cells have degraded or no DNA by 20-24 post-induction (PubMed:25277654).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3">Probably oligomeric upon overexpression; does not interact with a Cas5 (Sso1441)/Cas7 (Sso1442)/crRNA complex (PubMed:23846216). Forms dimer and tetramer-sized oligomers when overexpressed in the absence of CRISPR loci A-D; the oligomers are probably responsible for its toxicity in the absence of CRISPR loci A-D (PubMed:25277654). Part of the aCascade ribonucleoprotein complex, minimally composed of Cas7 (formerly Csa2) and Cas5a, which binds crRNA. Other possible components of aCascade in strain P1 are Cas6b (SSO1437) and Csa5 (SSO1443), while SSO1399, Cas5b (SSO1400) and SSO1401 have sometimes been seen weakly associated. Csa2 is probably the major RNA-binding subunit. The Csa2-Cas5a-crRNA complex also binds target DNA homologous to crRNA, probably forming an R-loop. Purified aCascade forms a filament about 6 nm in width (PubMed:21507944).</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The aCascade complex was purified from strain P1.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90302">
        <property type="entry name" value="E90302"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923408.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9CRO">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="I/J/K/L/M/N=1-150"/>
    </dbReference>
    <dbReference type="PDB" id="9CRP">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.20 A"/>
        <property type="chains" value="H/J/K/L/N=1-150"/>
    </dbReference>
    <dbReference type="PDB" id="9CRQ">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.07 A"/>
        <property type="chains" value="H/K/L/N=1-150"/>
    </dbReference>
    <dbReference type="PDBsum" id="9CRO"/>
    <dbReference type="PDBsum" id="9CRP"/>
    <dbReference type="PDBsum" id="9CRQ"/>
    <dbReference type="AlphaFoldDB" id="Q97Y90"/>
    <dbReference type="EMDB" id="EMD-45873"/>
    <dbReference type="EMDB" id="EMD-45874"/>
    <dbReference type="EMDB" id="EMD-45875"/>
    <dbReference type="SMR" id="Q97Y90"/>
    <dbReference type="STRING" id="273057.SSO1443"/>
    <dbReference type="PaxDb" id="273057-SSO1443"/>
    <dbReference type="EnsemblBacteria" id="AAK41676">
        <property type="protein sequence ID" value="AAK41676"/>
        <property type="gene ID" value="SSO1443"/>
    </dbReference>
    <dbReference type="GeneID" id="1454455"/>
    <dbReference type="GeneID" id="27427811"/>
    <dbReference type="KEGG" id="sso:SSO1443"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1472"/>
    <dbReference type="eggNOG" id="arCOG03823">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1811533_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y90"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010157">
        <property type="entry name" value="CRISPR-assoc_Cas5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01878">
        <property type="entry name" value="cas_Csa5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09702">
        <property type="entry name" value="Cas_Csa5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0800">Toxin</keyword>
    <feature type="chain" id="PRO_0000417888" description="CRISPR type I-A cluster 2/Apern-associated protein Csa5-2">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="In strain P2 protein is soluble upon overexpression, behaves as a monomer. No longer toxic when expressed in vivo in the absence of CRISPR loci A-D." evidence="2 3">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="21507944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="23846216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="25277654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="25277654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="150" mass="16487" checksum="50D18F528A157F1B" modified="2001-10-01" version="1">MAQKSEKENIIGRIANLLAVGFLYSESPTLVDRFANALSKEAVTKVLYDVQRIVQMGIDRSEIATTTITIQGKDYPAQGKDYPAVNVNSSGAKYTVVGYLPTSQDIEDFLRMIEEDVYYARKAGALAMSIANRIKLGSKQSKSEQGGEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YG0</accession>
    <name>Q97YG0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1048</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90292">
        <property type="entry name" value="G90292"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YG0"/>
    <dbReference type="STRING" id="273057.SSO1363"/>
    <dbReference type="PaxDb" id="273057-SSO1363"/>
    <dbReference type="EnsemblBacteria" id="AAK41598">
        <property type="protein sequence ID" value="AAK41598"/>
        <property type="gene ID" value="SSO1363"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1363"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1369"/>
    <dbReference type="eggNOG" id="arCOG02129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056788_15_2_2"/>
    <dbReference type="InParanoid" id="Q97YG0"/>
    <dbReference type="PhylomeDB" id="Q97YG0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="63"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="232" mass="27010" checksum="72C710E0F2F7ADE5" modified="2001-10-01" version="1">MDNENTQDSTTRTRNRIQEIQTIRTSPRTRIQPSKTQTYQHPSRKGQVGRFLKKIKKLERKALFFLDECRTVISTSIKKVLAKVGSKPVMRVNIGFSSIYVILAINAWTGEVVVSLAERPNSESVKYFLRYFKRRVGSGRVYMVMDNYSPHKTKGTLEVCRRKGIHPVFTPPYSPELNMAEAVFKSLKNYMSNKIFYTIEDVKNCIKQFFEENKYRFNLNAITYLGLDKIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YH2</accession>
    <name>Q97YH2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">First ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1349</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90291">
        <property type="entry name" value="B90291"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923342.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YH2"/>
    <dbReference type="STRING" id="273057.SSO1349"/>
    <dbReference type="PaxDb" id="273057-SSO1349"/>
    <dbReference type="EnsemblBacteria" id="AAK41585">
        <property type="protein sequence ID" value="AAK41585"/>
        <property type="gene ID" value="SSO1349"/>
    </dbReference>
    <dbReference type="GeneID" id="1454364"/>
    <dbReference type="KEGG" id="sso:SSO1349"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1355"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="InParanoid" id="Q97YH2"/>
    <dbReference type="PhylomeDB" id="Q97YH2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04762">
        <property type="entry name" value="HTH_MerR-trunc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1660.10:FF:000013">
        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2170:FF:000002">
        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1390:FF:000002">
        <property type="entry name" value="ORF1 in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="5">
        <location>
            <begin position="55"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="195" mass="22911" checksum="27026474C8BAC890" modified="2001-10-01" version="1">MLRPKEACQRLGISYATLREYVKKGYIKPVILQSGKWRFREEDIERLMGIIRKRKVILYARVSSSTQKDDLVNQVKYLEEQVKEYDQVITDIGSGLNMKRKGFLKLLRMILNNEVSRVVVAYPDRLVRFGFEILEEVCKAHNCEIVVLNQEDKEEELVEDLMSALVSFSGKLYGMRSHKYEKVKKCAEELKNWKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YJ0</accession>
    <name>Q97YJ0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Schlafen group 3-like DNA/RNA helicase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90289">
        <property type="entry name" value="B90289"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923326.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YJ0"/>
    <dbReference type="STRING" id="273057.SSO1331"/>
    <dbReference type="PaxDb" id="273057-SSO1331"/>
    <dbReference type="EnsemblBacteria" id="AAK41569">
        <property type="protein sequence ID" value="AAK41569"/>
        <property type="gene ID" value="SSO1331"/>
    </dbReference>
    <dbReference type="GeneID" id="1454348"/>
    <dbReference type="KEGG" id="sso:SSO1331"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1335"/>
    <dbReference type="eggNOG" id="arCOG04708">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019642_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YJ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018647">
        <property type="entry name" value="SLFN_3-like_DNA/RNA_helicase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09848">
        <property type="entry name" value="SLFN-g3_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Schlafen group 3-like DNA/RNA helicase" evidence="1">
        <location>
            <begin position="225"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="604" mass="69867" checksum="CB733E807EC80B6A" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YL4</accession>
    <name>Q97YL4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transglutaminase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90286">
        <property type="entry name" value="B90286"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923302.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YL4"/>
    <dbReference type="STRING" id="273057.SSO1309"/>
    <dbReference type="PaxDb" id="273057-SSO1309"/>
    <dbReference type="EnsemblBacteria" id="AAK41545">
        <property type="protein sequence ID" value="AAK41545"/>
        <property type="gene ID" value="SSO1309"/>
    </dbReference>
    <dbReference type="GeneID" id="1454324"/>
    <dbReference type="KEGG" id="sso:SSO1309"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1310"/>
    <dbReference type="eggNOG" id="arCOG02165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008973_1_0_2"/>
    <dbReference type="InParanoid" id="Q97YL4"/>
    <dbReference type="PhylomeDB" id="Q97YL4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013589">
        <property type="entry name" value="Bac_transglu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33490">
        <property type="entry name" value="BLR5614 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33490:SF6">
        <property type="entry name" value="SLL1049 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08379">
        <property type="entry name" value="Bact_transglu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="1">
        <location>
            <begin position="159"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="266" mass="30445" checksum="28FCAD9D90CC3479" modified="2001-10-01" version="1">MDYKVEYISIYEYEADVISNENTLKIVPYNGDNQVLISHEVGTIPNGYKTSYVDIFGNIVYKIKVQELHRRLEIYSKSVVTLDKKVIKTDYEFPYNYGFNEFLLPTRLVDPEQFQKIAKELTKSLKTLGEVIETIVNFVRKKIIYKPGITNINTTAFEAYSLGYGVCQDYTHITLGLLRALGIPARYVMGVVNDNPRATHAWVEVLTPDGTYIDVDPTRGRFYNLDYIKFAVGRDFNDVSPVIGFFVSSGRGWLKEVRIKVEKSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YP9</accession>
    <name>Q97YP9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligo/dipeptide transport, permease protein (DppC-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dppC-1</name>
        <name evidence="7" type="ordered locus">SSO1275</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99282">
        <property type="entry name" value="A99282"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990034.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YP9"/>
    <dbReference type="FunCoup" id="Q97YP9">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1275"/>
    <dbReference type="PaxDb" id="273057-SSO1275"/>
    <dbReference type="EnsemblBacteria" id="AAK41512">
        <property type="protein sequence ID" value="AAK41512"/>
        <property type="gene ID" value="SSO1275"/>
    </dbReference>
    <dbReference type="GeneID" id="1454291"/>
    <dbReference type="KEGG" id="sso:SSO1275"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1272"/>
    <dbReference type="eggNOG" id="arCOG00749">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_10_0_2"/>
    <dbReference type="InParanoid" id="Q97YP9"/>
    <dbReference type="PhylomeDB" id="Q97YP9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43839">
        <property type="entry name" value="OPPC IN A BINDING PROTEIN-DEPENDENT TRANSPORT SYSTEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43839:SF1">
        <property type="entry name" value="OPPC IN A BINDING PROTEIN-DEPENDENT TRANSPORT SYSTEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="311"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="417"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="248"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="450" mass="49332" checksum="418EB6565D63E851" modified="2001-10-01" version="1">MSKRNVGFSSYIRDFLSSKIGLVGLIILIILLIFTGIALSIPAKVYVSWNNPAAWNQYPEHVPPAWLSIFYPGKYFTTEELSPINMSLYSPAKNIFVAIITYQFNWSKVLPAYNVYFITSSNVTPIEEVIYWSKPDGYTLQVNVPAGVFNVPFDLTSIRGDILSYISSITGKTPNIVNSTVLSSALFNSPKSNFTVIEQGKYVVKIEIVSSLPMNITKSRLILLGNSYGLMGTDYFGRPLDLGILLGLPNALEIGVLTSLVAVLVGVFVGGISGYLGGNKDSIIQWVTLVFLALPALPFLVAVSFVAEPNIVIEALLIAFLSWPFYAIIARSMALSIKSNSYVEADKLLGIPSYRVFFTHFMPRLIPITVAYMVLGVPAGILLAQTLAFLGIAPANIVTWGGILDAAETYQAQVNGWWWWVVFPGAMVVIVAIPFVLIGFAIERVTLGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YS8</accession>
    <name>Q97YS8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Toluene-4-monooxygenase system protein C. (TmoC)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tmoC</name>
        <name evidence="7" type="ordered locus">SSO1230</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90277">
        <property type="entry name" value="E90277"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992602.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YS8"/>
    <dbReference type="FunCoup" id="Q97YS8">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1230"/>
    <dbReference type="PaxDb" id="273057-SSO1230"/>
    <dbReference type="EnsemblBacteria" id="AAK41476">
        <property type="protein sequence ID" value="AAK41476"/>
        <property type="gene ID" value="SSO1230"/>
    </dbReference>
    <dbReference type="GeneID" id="1454255"/>
    <dbReference type="KEGG" id="sso:SSO1230"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1230"/>
    <dbReference type="eggNOG" id="arCOG02852">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_5_0_2"/>
    <dbReference type="InParanoid" id="Q97YS8"/>
    <dbReference type="PhylomeDB" id="Q97YS8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.102.10.10:FF:000045">
        <property type="entry name" value="Rieske (2Fe-2S) iron-sulphur domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496">
        <property type="entry name" value="FERREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496:SF0">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="6">
        <location>
            <begin position="8"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="134" mass="15188" checksum="565094085F46C258" modified="2001-10-01" version="1">MNYPGEFHKLLTLDDIYEGEAKLVEVNRYEILVVNIKGEIKAYYARCAHALGLIESNSFDGEEKIICPVHLWEYNARTGESINPVGSSLFPLDVKVEGSDIFVKVPSVPISEFKVKWFGMYRQRGVINEFGVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YT0</accession>
    <name>Q97YT0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Toluene-4-monooxygenase system protein A. carboxy end (TmoA)</fullName>
            <ecNumber evidence="2">1.14.13.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tmoA</name>
        <name evidence="2" type="ordered locus">SSO1228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.13.-" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90277">
        <property type="entry name" value="C90277"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992600.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YT0"/>
    <dbReference type="FunCoup" id="Q97YT0">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1228"/>
    <dbReference type="PaxDb" id="273057-SSO1228"/>
    <dbReference type="EnsemblBacteria" id="AAK41474">
        <property type="protein sequence ID" value="AAK41474"/>
        <property type="gene ID" value="SSO1228"/>
    </dbReference>
    <dbReference type="GeneID" id="1454253"/>
    <dbReference type="KEGG" id="sso:SSO1228"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1228"/>
    <dbReference type="eggNOG" id="arCOG04507">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_896079_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YT0"/>
    <dbReference type="PhylomeDB" id="Q97YT0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007029">
        <property type="entry name" value="YHS_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04945">
        <property type="entry name" value="YHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YHS" evidence="1">
        <location>
            <begin position="197"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="310" mass="36599" checksum="176917884F145E34" modified="2001-10-01" version="1">MSREGSLGQTKGEVKQVLSNISEGLMKNYRNTVEFAARMREKGPAYKEAGEYLVAKGFWLSVRLIGALTGVSMDYLTPLDARIMSYKEFMTEWVGAQLKRLLEDYGIRLPWYWKWFELELDHWHHDFIIGLYTWRRTLNVSFRGPTPDERKWLNEKYPHWEMFFGRVWDLYIKKIIDGQIPLPLTAVHLCAVCQVPIQAPANGKYLRIYLKEYKGKMYTFDSPACLWIFEQEPERYAGRRTYTQRVLEGMIQFTEEAYKDPKRLLDEVIWNMGQTEEGEAGLDPTDGAYALLYREKDPDFFNRIKKYTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YT2</accession>
    <name>Q97YT2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Toluene 1,2-dioxygenase system ferredoxin--NAD(+) reductase component, probable (TodA)</fullName>
            <ecNumber evidence="7">1.18.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">todA</name>
        <name evidence="7" type="ordered locus">SSO1225</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.18.1.3" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99277">
        <property type="entry name" value="A99277"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923263.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YT2"/>
    <dbReference type="FunCoup" id="Q97YT2">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1225"/>
    <dbReference type="PaxDb" id="273057-SSO1225"/>
    <dbReference type="EnsemblBacteria" id="AAK41472">
        <property type="protein sequence ID" value="AAK41472"/>
        <property type="gene ID" value="SSO1225"/>
    </dbReference>
    <dbReference type="GeneID" id="1454251"/>
    <dbReference type="KEGG" id="sso:SSO1225"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1226"/>
    <dbReference type="eggNOG" id="arCOG01070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003291_4_0_2"/>
    <dbReference type="InParanoid" id="Q97YT2"/>
    <dbReference type="PhylomeDB" id="Q97YT2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008860">
        <property type="term" value="F:ferredoxin-NAD+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050446">
        <property type="entry name" value="FAD-oxidoreductase/Apoptosis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028202">
        <property type="entry name" value="Reductase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557:SF2">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14759">
        <property type="entry name" value="Reductase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="5">
        <location>
            <begin position="5"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Reductase C-terminal" evidence="6">
        <location>
            <begin position="315"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="404" mass="45525" checksum="7510C7A59CF20F37" modified="2001-10-01" version="1">MKCEYLIIGSGIAGYNALKELLQIKPNSRIIMITSDKYYPYDRPPLSKDYLKGKLEKDMLFFESDDFYKRDNLEVMLNKSVERIDANLKEAILNDGSVISFDKALISTGGRPRRLNIPGSENALYLRSLDDADRIREAASKGKNALIIGAGFIGVEVASSLITLGVKTTVVEVMPYIWNTFVDEKVSRVIQQYLESKGINFILNESVKEIQGKIATTSSGRKIEADMFLIAVGISPNVELAQRSGMQVDNGIVVNEYLETSARDIYAAGDIANIFDPREGKRKRIEHWNNAEYTGKLAARNMAGSREAYNFISSIWSDIFDLHIESAGETRNYDEYIIRGKFELERPRFSVIYLKGGIIKGYLAINRNVKEIIALNKLIQKQADVSSKRDKLADESFDLQKLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z01</accession>
    <name>Q97Z01_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90267">
        <property type="entry name" value="G90267"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z01"/>
    <dbReference type="PaxDb" id="273057-SSO1147"/>
    <dbReference type="EnsemblBacteria" id="AAK41398">
        <property type="protein sequence ID" value="AAK41398"/>
        <property type="gene ID" value="SSO1147"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1147"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1139"/>
    <dbReference type="eggNOG" id="arCOG02692">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2299473_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z01"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="10918" checksum="5DAE4B9274826CDE" modified="2001-10-01" version="1">MILAGIVLSRRNFLPGEILMSISDSALYAPQSSIFTEIFDKKYRFTVSNFSYQAASILGGSLAPVILRANQSSLVLVTLSYVLVTFISVLLIAETKGKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z05</accession>
    <name>Q97Z05_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transport protein, permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1142</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90267">
        <property type="entry name" value="B90267"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923206.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z05"/>
    <dbReference type="FunCoup" id="Q97Z05">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1142"/>
    <dbReference type="PaxDb" id="273057-SSO1142"/>
    <dbReference type="EnsemblBacteria" id="AAK41393">
        <property type="protein sequence ID" value="AAK41393"/>
        <property type="gene ID" value="SSO1142"/>
    </dbReference>
    <dbReference type="GeneID" id="15297459"/>
    <dbReference type="KEGG" id="sso:SSO1142"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1133"/>
    <dbReference type="eggNOG" id="arCOG00147">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_745192_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z05"/>
    <dbReference type="PhylomeDB" id="Q97Z05"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17353">
        <property type="entry name" value="MFS_OFA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052983">
        <property type="entry name" value="MFS_Riboflavin_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43385">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER RIBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43385:SF1">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER RIBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="371" mass="40210" checksum="606955DF8E116B9F" modified="2001-10-01" version="1">MKRNKYIIIGFVIMCFNSLYQYSWNALEPLLREGFNVSVVEIALGFTLFSVFSSVFQPIGGHFADKQGPKRIGVISAILSAIGFLGTYLSPNIYVFFVSWSIGSIGEGILYGIAANLAMKWFRERMGFATGIVSMGFGIGSAIANPFISMAGNYRIVTLAIGLVEIVLLPILLYISDYPPKLSGQTPRQAVLSVKFWLIYLSFATSIVPLTVLSSELPILGKGLPQQELVTLISILPLLSGGLRPIFGYIADRLGILRTTFLLTIVLTLGSISLLLGEIALSTILVGFAGGSLITLYFNVSTEIFGFKFSTVNSGILYTGKALGGVLGSIIFALLYTISLRTSEIYTLSCSLIGVLTLLPLLFTKQSRKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z45</accession>
    <name>Q97Z45_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90261">
        <property type="entry name" value="H90261"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989725.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z45"/>
    <dbReference type="STRING" id="273057.SSO1091"/>
    <dbReference type="PaxDb" id="273057-SSO1091"/>
    <dbReference type="EnsemblBacteria" id="AAK41351">
        <property type="protein sequence ID" value="AAK41351"/>
        <property type="gene ID" value="SSO1091"/>
    </dbReference>
    <dbReference type="GeneID" id="1454130"/>
    <dbReference type="KEGG" id="sso:SSO1091"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1089"/>
    <dbReference type="eggNOG" id="arCOG02900">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058577_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z45"/>
    <dbReference type="PhylomeDB" id="Q97Z45"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040929">
        <property type="entry name" value="ArnB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737:SF6">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18677">
        <property type="entry name" value="ArnB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="39"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="380" mass="42755" checksum="B376F7BA3BE1C81B" modified="2001-10-01" version="1">MTLSLRVDTSHKYSFNADVRYIFKVLLVPEKLGSATGFHYIVALDTSGSMTGYKIELAKQGAIELFKRIPNGNKVSFITFSSNVNVIKEFVDPLDLTNEILQITAGGQTALYTAILTANSLAKKYQMPTYLLLLTDGNPTDETNIGNYLKLPYYEKIQVYSFGIGDDYNEQLLQSVSDKTGGVMYHISDANEIPQKLPQKAVTQIAAKNVTVDITAEGNVKLLNYVTTPVKVNGIENVIKIFGETILPANYEGNFLTVKVNYEDPVTNKPESLLQVIQVRKAQDQNTFVSGINNDVINEYRYYELLDKYAKQVQAEQLVEATKTLNQLNEIAQQTRRIDFMETTRRLSEGLETTKRIGTVEQTKRLSKEVTSEVTRKLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z52</accession>
    <name>Q97Z52_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glutaconate CoA-transferase, subunit A (GctA)</fullName>
            <ecNumber evidence="1">2.8.3.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gctA</name>
        <name evidence="1" type="ordered locus">SSO1083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.3.12" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99261">
        <property type="entry name" value="A99261"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z52"/>
    <dbReference type="FunCoup" id="Q97Z52">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1083"/>
    <dbReference type="PaxDb" id="273057-SSO1083"/>
    <dbReference type="EnsemblBacteria" id="AAK41344">
        <property type="protein sequence ID" value="AAK41344"/>
        <property type="gene ID" value="SSO1083"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1083"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1082"/>
    <dbReference type="eggNOG" id="arCOG01987">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049557_1_0_2"/>
    <dbReference type="InParanoid" id="Q97Z52"/>
    <dbReference type="PhylomeDB" id="Q97Z52"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018730">
        <property type="term" value="F:glutaconate CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43293">
        <property type="entry name" value="ACETATE COA-TRANSFERASE YDIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43293:SF3">
        <property type="entry name" value="CHOLESTEROL RING-CLEAVING HYDROLASE IPDB SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="268" mass="30368" checksum="FDAD83736A860B52" modified="2001-10-01" version="1">MEEVFIYIFFVEIINMESKLLSLEEAVEVVKKGDSVTISGISIHRNPMAFIYALVKKGVRDLYFIDREPGFGLEVLLKYGLIRKLRIAMSTLEWFSSIPKHFRKMTEEREVEILEDTCGAFIAGIRAGSFGIPFMPVRGIIGSDLVKIHEREGTWKVVEDPFSGEKVVLVKAINPDVAIIHVNKADEEGNAEILGPLYEDEYKAKAAKKVIITAEEIVPRSYFYGRRPTINGEYVTAVVHAPKGAEPTSMFPLYDADYEKIIEFLEHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZB8</accession>
    <name>Q97ZB8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1177 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90252">
        <property type="entry name" value="D90252"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989233.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZB8"/>
    <dbReference type="STRING" id="273057.SSO1005"/>
    <dbReference type="PaxDb" id="273057-SSO1005"/>
    <dbReference type="EnsemblBacteria" id="AAK41275">
        <property type="protein sequence ID" value="AAK41275"/>
        <property type="gene ID" value="SSO1005"/>
    </dbReference>
    <dbReference type="GeneID" id="1455242"/>
    <dbReference type="KEGG" id="sso:SSO1005"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1001"/>
    <dbReference type="eggNOG" id="arCOG05881">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077081_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZB8"/>
    <dbReference type="PhylomeDB" id="Q97ZB8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009561">
        <property type="entry name" value="DUF1177"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06675">
        <property type="entry name" value="DUF1177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="32909" checksum="E06C7CB22CB6C152" modified="2001-10-01" version="1">MILKTLLEVIDILESKDPLQKIRERLESKVKYEEIKAGEVTFIKALYEGVGNNKVEILGRLGAIQMVGVNKGLVSDADGAIISLAVLLELLNLRDKGIELNLDVLFVTNLSIKAKLIPHKPFDFMVPLVGLDEALKVEVEPSASLVISIDSTKGNRIVKFDDFAITHVVKDGYILKLHDNVIDIYNKITGHEIYMVPLTTGDLTPLDYNVSHISTLISPWLYTDSPVIGIATVSKQVIPGYETGVLNIEMLEHASRFCIELLKYIENGGKVYEEKELEELENRLGKSSLLKAKRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-08-29" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZG0</accession>
    <name>GATC_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Glutamyl-tRNA(Gln) amidotransferase subunit C</fullName>
            <shortName>Glu-ADT subunit C</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatC</name>
        <name type="ordered locus">SSO6855</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-aspartyl-tRNA(Asn) + L-glutamine + ATP + H2O = L-asparaginyl-tRNA(Asn) + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90247">
        <property type="entry name" value="A90247"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992398.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZG0"/>
    <dbReference type="SMR" id="Q97ZG0"/>
    <dbReference type="FunCoup" id="Q97ZG0">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO6855"/>
    <dbReference type="PaxDb" id="273057-SSO6855"/>
    <dbReference type="EnsemblBacteria" id="AAK41232">
        <property type="protein sequence ID" value="AAK41232"/>
        <property type="gene ID" value="SSO6855"/>
    </dbReference>
    <dbReference type="GeneID" id="1455199"/>
    <dbReference type="GeneID" id="44129887"/>
    <dbReference type="KEGG" id="sso:SSO6855"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.954"/>
    <dbReference type="eggNOG" id="arCOG02726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105899_4_1_2"/>
    <dbReference type="InParanoid" id="Q97ZG0"/>
    <dbReference type="PhylomeDB" id="Q97ZG0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.60">
        <property type="entry name" value="Glu-tRNAGln amidotransferase C subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00122">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="GatC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00135">
        <property type="entry name" value="gatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000684">
        <property type="entry name" value="PRK00034.3-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="Glu-tRNAGln amidotransferase C subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000105361" description="Glutamyl-tRNA(Gln) amidotransferase subunit C">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00122"/>
        </source>
    </evidence>
    <sequence length="97" mass="11306" checksum="CABDD04A4D1AD86F" modified="2001-08-29" version="1">MKIEVNENLIKHLENLSLIQLSGEEEKILEKDLANIIKFFEKINELDLSNVEPLFHPLPQGRLRKDVPRDPLDRENALKNVKRKEDGYIVGPRTYGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZM6</accession>
    <name>Q97ZM6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90237">
        <property type="entry name" value="C90237"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992285.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZM6"/>
    <dbReference type="STRING" id="273057.SSO0865"/>
    <dbReference type="PaxDb" id="273057-SSO0865"/>
    <dbReference type="EnsemblBacteria" id="AAK41154">
        <property type="protein sequence ID" value="AAK41154"/>
        <property type="gene ID" value="SSO0865"/>
    </dbReference>
    <dbReference type="GeneID" id="1455121"/>
    <dbReference type="KEGG" id="sso:SSO0865"/>
    <dbReference type="eggNOG" id="arCOG04160">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084972_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZM6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="276" mass="31819" checksum="4CFE54C6E8CFD84D" modified="2001-10-01" version="1">MPRRKKNEEGKADSEKNTSKEEIRKKKSSSSKKAEKYIDPDKVLDEYLNEIVNALGISYLNLSVDDLKEVLREPFASAVGEVKTKPKVSTIINRLKAMGDRLMEIISYKLLRLYDVEKLSEDQLEFIVTYGKGGLIPIMDKLYKECLKRNRKDLIDLLRVTWSMFASILRSPIKCPKCEFDSVMPDLSCRVCGYMLSMKELKNTIHVIDILQDFLKMDRDGFYEILKSGFFYYTSEGPIPPSRFKPTQGQIYFEVILNKEEKSKLESISHSILPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZP2</accession>
    <name>Q97ZP2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transposase IS110-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO6659</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99235">
        <property type="entry name" value="A99235"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZP2"/>
    <dbReference type="STRING" id="273057.SSO6659"/>
    <dbReference type="PaxDb" id="273057-SSO6659"/>
    <dbReference type="EnsemblBacteria" id="AAK41136">
        <property type="protein sequence ID" value="AAK41136"/>
        <property type="gene ID" value="SSO6659"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO6659"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.856"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166569_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZP2"/>
    <dbReference type="PhylomeDB" id="Q97ZP2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="1">
        <location>
            <begin position="23"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="94" mass="10740" checksum="24B3C08D1A765ADB" modified="2001-10-01" version="1">MKTPVAGINISKDKLIVYFQGKFYEFPNDKQGFEEVMPRGCKVGIKSTGVYHVNLAKYEVRVINPLVIKKFKDFRGKKSDKNDAKKLAELVVNM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZQ4</accession>
    <name>RL23_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L23</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl23</name>
        <name type="ordered locus">SSO6401</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts protein L29.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99220">
        <property type="entry name" value="A99220"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991280.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZQ4"/>
    <dbReference type="SMR" id="Q97ZQ4"/>
    <dbReference type="FunCoup" id="Q97ZQ4">
        <property type="interactions" value="180"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO6401"/>
    <dbReference type="PaxDb" id="273057-SSO6401"/>
    <dbReference type="EnsemblBacteria" id="AAK41016">
        <property type="protein sequence ID" value="AAK41016"/>
        <property type="gene ID" value="SSO6401"/>
    </dbReference>
    <dbReference type="GeneID" id="1454983"/>
    <dbReference type="KEGG" id="sso:SSO6401"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.716"/>
    <dbReference type="eggNOG" id="arCOG04072">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037562_4_2_2"/>
    <dbReference type="InParanoid" id="Q97ZQ4"/>
    <dbReference type="PhylomeDB" id="Q97ZQ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001084">
        <property type="entry name" value="50S ribosomal protein L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019985">
        <property type="entry name" value="Ribosomal_uL23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001014">
        <property type="entry name" value="Ribosomal_uL23_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011118">
        <property type="entry name" value="PRK14548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03636">
        <property type="entry name" value="uL23_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000272956" description="Large ribosomal subunit protein uL23">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="81" mass="9127" checksum="7A651776FCA0893E" modified="2001-10-01" version="1">MIQMALATEKALKLIESYNTLTLIVDKSDTKDDIKKSVERLFNVKVVKVNVVITPQGYKKAYVKLAPEYKASDIAHKLGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q980C4</accession>
    <name>Q980C4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase small domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0392</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90182">
        <property type="entry name" value="H90182"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988805.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980C4"/>
    <dbReference type="STRING" id="273057.SSO0392"/>
    <dbReference type="PaxDb" id="273057-SSO0392"/>
    <dbReference type="EnsemblBacteria" id="AAK40719">
        <property type="protein sequence ID" value="AAK40719"/>
        <property type="gene ID" value="SSO0392"/>
    </dbReference>
    <dbReference type="GeneID" id="1455529"/>
    <dbReference type="KEGG" id="sso:SSO0392"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.386"/>
    <dbReference type="eggNOG" id="arCOG00110">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_7_2_2"/>
    <dbReference type="InParanoid" id="Q980C4"/>
    <dbReference type="PhylomeDB" id="Q980C4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008990">
        <property type="term" value="F:rRNA (guanine-N2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046977">
        <property type="entry name" value="RsmC/RlmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47816">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47816:SF4">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="3">
        <location>
            <begin position="16"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="186" mass="20872" checksum="A83E196EC146FA98" modified="2001-10-01" version="1">MSHEYIIFEVINGIPLTLLSSPGIFSKKELDLGTRILLENIKVAENGTVADVGCGYGPIGIYLALKNPNLKVYMVDVNYIAIKLAKKNAKLNGVENRTIIVKSDIFDNIPIDVKFTAIYSNPPLSKGVDFLKKLEAEAYDRLVEKGFIQLIAYKGEENIEKIFSKRFKIESIKRKKGYSLITIVKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2018-05-23" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q980C6</accession>
    <name>SLAB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">S-layer protein B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Surface layer small protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">slaB</name>
        <name evidence="6" type="ordered locus">SSO0390</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2009" name="Mol. Microbiol." volume="73" first="58" last="72">
            <title>Acidianus, Sulfolobus and Metallosphaera surface layers: structure, composition and gene expression.</title>
            <authorList>
                <person name="Veith A."/>
                <person name="Klingl A."/>
                <person name="Zolghadr B."/>
                <person name="Lauber K."/>
                <person name="Mentele R."/>
                <person name="Lottspeich F."/>
                <person name="Rachel R."/>
                <person name="Albers S.V."/>
                <person name="Kletzin A."/>
            </authorList>
            <dbReference type="PubMed" id="19522740"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.2009.06746.x"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 25-31</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>INDUCTION</scope>
        <scope>DOMAIN</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">S-layer small protein. May anchor the complex to the cell membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The mushroom-shaped unit cells of the Sulfolobales' S-layers may consist of three SlaB subunits and six SlaA subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Secreted</location>
            <location evidence="5">Cell wall</location>
            <location evidence="5">S-layer</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="2">Constitutively expressed.</text>
    </comment>
    <comment type="domain">
        <text evidence="2">Composed of consecutive beta sandwich domains, a coiled-coil domain and a C-terminal transmembrane helix.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Sulfolobales SlaB family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90182">
        <property type="entry name" value="F90182"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922961.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980C6"/>
    <dbReference type="SMR" id="Q980C6"/>
    <dbReference type="STRING" id="273057.SSO0390"/>
    <dbReference type="PaxDb" id="273057-SSO0390"/>
    <dbReference type="EnsemblBacteria" id="AAK40717">
        <property type="protein sequence ID" value="AAK40717"/>
        <property type="gene ID" value="SSO0390"/>
    </dbReference>
    <dbReference type="GeneID" id="1455527"/>
    <dbReference type="GeneID" id="44129367"/>
    <dbReference type="KEGG" id="sso:SSO0390"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.384"/>
    <dbReference type="eggNOG" id="arCOG07272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_697598_0_0_2"/>
    <dbReference type="InParanoid" id="Q980C6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030115">
        <property type="term" value="C:S-layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58100">
        <property type="entry name" value="Bacterial hemolysins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0134">Cell wall</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0701">S-layer</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000444055" description="S-layer protein B">
        <location>
            <begin position="25"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="259"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="19522740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="19522740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="19522740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="397" mass="41546" checksum="01C2618BA0D7C2DA" modified="2001-10-01" version="1" precursor="true">MVVKKTFVLSTLILISVVALVSTAVYTSGNVTFYSPSVNNQIYYVGKSVTIDAVVPQQFAGQGAVINLFFPNSTLAASIPTTVNATGGIYVPNAYTFPHVIGIWQITVEVTGGVAVGTIDVNVTTPAVAPIILTLQNLAMYENTYPQFIEFANGIITSVVMQNGTVNIMGYVYNSTVGPLSGATVSLTLNIPTVGTKTLSTTTASNGSFMLSFQVPQLSTTLTLISSYLISGSLTVSYGVHTVTYNVFITAIPNYLSVINALNNEVSTLRSEISSLNSTIASLNKSLANANTQISNLQSEITTLNSEIGKLNSTVGSLSTQLSSLSSQYNTLSSQVTALNGKISNLSTSLSTLNGEVASLRSTVSSLTTIAYGGIIAGIIGLIVAIVAIVLVMRRIS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2018-05-23" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q980C7</accession>
    <accession>Q2M1E7</accession>
    <name>SLAA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">S-layer protein A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Surface layer large protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">slaA</name>
        <name evidence="6" type="synonym">slp1</name>
        <name evidence="5" type="ordered locus">SSO0389</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Can. J. Microbiol." volume="51" first="731" last="743">
            <title>Molecular organization of selected prokaryotic S-layer proteins.</title>
            <authorList>
                <person name="Claus H."/>
                <person name="Akca E."/>
                <person name="Debaerdemaeker T."/>
                <person name="Evrard C."/>
                <person name="Declercq J.P."/>
                <person name="Harris J.R."/>
                <person name="Schlott B."/>
                <person name="Konig H."/>
            </authorList>
            <dbReference type="PubMed" id="16391651"/>
            <dbReference type="DOI" id="10.1139/w05-093"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2009" name="Mol. Microbiol." volume="73" first="58" last="72">
            <title>Acidianus, Sulfolobus and Metallosphaera surface layers: structure, composition and gene expression.</title>
            <authorList>
                <person name="Veith A."/>
                <person name="Klingl A."/>
                <person name="Zolghadr B."/>
                <person name="Lauber K."/>
                <person name="Mentele R."/>
                <person name="Lottspeich F."/>
                <person name="Rachel R."/>
                <person name="Albers S.V."/>
                <person name="Kletzin A."/>
            </authorList>
            <dbReference type="PubMed" id="19522740"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.2009.06746.x"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 36-41</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>INDUCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-layer large protein. May form the highly ordered outer sheath.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The mushroom-shaped unit cells of the Sulfolobales' S-layers may consist of three SlaB subunits and six SlaA subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
            <location evidence="1">S-layer</location>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="1">Constitutively expressed.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Sulfolobales SlaA family.</text>
    </comment>
    <dbReference type="EMBL" id="BN000829">
        <property type="protein sequence ID" value="CAJ31324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90182">
        <property type="entry name" value="E90182"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922960.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980C7"/>
    <dbReference type="FunCoup" id="Q980C7">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="IntAct" id="Q980C7">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="MINT" id="Q980C7"/>
    <dbReference type="STRING" id="273057.SSO0389"/>
    <dbReference type="PaxDb" id="273057-SSO0389"/>
    <dbReference type="EnsemblBacteria" id="AAK40716">
        <property type="protein sequence ID" value="AAK40716"/>
        <property type="gene ID" value="SSO0389"/>
    </dbReference>
    <dbReference type="GeneID" id="1455526"/>
    <dbReference type="GeneID" id="27426684"/>
    <dbReference type="KEGG" id="sso:SSO0389"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.383"/>
    <dbReference type="eggNOG" id="arCOG06039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_264979_0_0_2"/>
    <dbReference type="InParanoid" id="Q980C7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030115">
        <property type="term" value="C:S-layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0134">Cell wall</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0701">S-layer</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000444052" description="S-layer protein A">
        <location>
            <begin position="36"/>
            <end position="1231"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="19522740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="19522740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="19522740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="CAJ31324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="1231" mass="131868" checksum="B85623012F8BCAEE" modified="2001-10-01" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2017-09-27" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q980D1</accession>
    <name>SYTE_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Threonine--tRNA ligase editing subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ser-tRNA(Thr) hydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Threonyl-tRNA synthetase editing subunit</fullName>
            <shortName evidence="3">ThrS-ed</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">thrS-ed</name>
        <name type="ordered locus">SSO0384</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="Proc. Natl. Acad. Sci. U.S.A." volume="101" first="10260" last="10265">
            <title>A freestanding proofreading domain is required for protein synthesis quality control in Archaea.</title>
            <authorList>
                <person name="Korencic D."/>
                <person name="Ahel I."/>
                <person name="Schelert J."/>
                <person name="Sacher M."/>
                <person name="Ruan B."/>
                <person name="Stathopoulos C."/>
                <person name="Blum P."/>
                <person name="Ibba M."/>
                <person name="Soell D."/>
            </authorList>
            <dbReference type="PubMed" id="15240874"/>
            <dbReference type="DOI" id="10.1073/pnas.0403926101"/>
        </citation>
        <scope>FUNCTION IN EDITING</scope>
        <scope>SUBUNIT</scope>
        <scope>DISRUPTION PHENOTYPE</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Freestanding tRNA editing subunit of threonine--tRNA ligase, the catalytic subunit is AC Q97VW8. Deacylates (edits) mischarged L-seryl-tRNA(Thr) in trans, removing L-serine, has no aminoacylation activity. In vitro when both subunits are present, or if the 2 subunits are fused, L-seryl-tRNA(Thr) is no longer produced. Has no activity on correctly acylated L-seryl-tRNA(Ser) or L-threonyl-tRNA(Thr) (PubMed:15240874). Editing is probably catalyzed by the 2'-OH of A76 of tRNA(Thr).</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Probably interacts with its catalytic subunit (AC Q97VW8); a subunit fusion (in the order edit-catalytic) is fully functional.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="disruption phenotype">
        <text evidence="2">Not essential, however growth is severely inhibited in the presence of 0.5 mM L-serine, but not in the presence of 0.5 mM L-ser plus 0.5 mM L-threonine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-II aminoacyl-tRNA synthetase family. Archaea-specific ThrRS editing domain subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90182">
        <property type="entry name" value="A90182"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988816.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980D1"/>
    <dbReference type="SMR" id="Q980D1"/>
    <dbReference type="STRING" id="273057.SSO0384"/>
    <dbReference type="PaxDb" id="273057-SSO0384"/>
    <dbReference type="EnsemblBacteria" id="AAK40712">
        <property type="protein sequence ID" value="AAK40712"/>
        <property type="gene ID" value="SSO0384"/>
    </dbReference>
    <dbReference type="GeneID" id="1455522"/>
    <dbReference type="KEGG" id="sso:SSO0384"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.378"/>
    <dbReference type="eggNOG" id="arCOG00401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_712936_0_0_2"/>
    <dbReference type="InParanoid" id="Q980D1"/>
    <dbReference type="PhylomeDB" id="Q980D1"/>
    <dbReference type="BRENDA" id="6.1.1.3">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000001">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.80.10:FF:000004">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="entry name" value="D-tyrosyl-tRNA(Tyr) deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015011">
        <property type="entry name" value="Threonyl-tRNA_syn_edit_dom_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011500">
        <property type="entry name" value="PRK14938.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF44">
        <property type="entry name" value="THREONINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08915">
        <property type="entry name" value="tRNA-Thr_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000441620" description="Threonine--tRNA ligase editing subunit">
        <location>
            <begin position="1"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9YFY3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15240874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="15240874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="15240874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="386" mass="44954" checksum="8707FCE32EF29128" modified="2001-10-01" version="1">MIILFIHASDFSFNVKERAIKEPEEAKLKSIELKNTLVCFTTVEKGDDEEILSKAIDDILDVYSKVKADSVVIYPYAHLSSNLANPDTAIKILESLENLLKDKVKVYRAPFGWYKAFSISCYGHPLSELSRRIRKTEELEKSEELKYCEKFGFPSSSESAFMRRATIGYLRNLFQPLFESENNENVRDGEMSILYQNVESGRILPCINENPRIVVVYGGVRELNFPKEINDSKNRIRVWWVNESKIYVDVGRLIYYFILESVKQQPPTLPDWLNPIQVRLLPVKKDFLDFSIQVAERLRKEGIRVNIDDLDDSLGNKIRRAGTDWIPFVIVIGEREVKTNTLTVKIRARNEQKSMTVEELVKEIKDEVKERQNLPLYYTLYRHKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q980E3</accession>
    <name>Q980E3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta-ribofuranosylaminobenzene 5'-phosphate synthase</fullName>
            <shortName evidence="2">Beta-RFA-P synthase</shortName>
            <ecNumber evidence="2">2.4.2.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0370</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of 4-aminobenzoate (pABA) with 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to produce beta-ribofuranosylaminobenzene 5'-phosphate (beta-RFA-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + 4-hydroxybenzoate + H(+) = 4-(beta-D-ribofuranosyl)phenol 5'-phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:48556"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:82767"/>
            <dbReference type="EC" id="2.4.2.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-RFA-P synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.54" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90180">
        <property type="entry name" value="E90180"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990672.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980E3"/>
    <dbReference type="STRING" id="273057.SSO0370"/>
    <dbReference type="PaxDb" id="273057-SSO0370"/>
    <dbReference type="EnsemblBacteria" id="AAK40700">
        <property type="protein sequence ID" value="AAK40700"/>
        <property type="gene ID" value="SSO0370"/>
    </dbReference>
    <dbReference type="GeneID" id="1455510"/>
    <dbReference type="KEGG" id="sso:SSO0370"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.364"/>
    <dbReference type="eggNOG" id="arCOG01026">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061764_0_0_2"/>
    <dbReference type="InParanoid" id="Q980E3"/>
    <dbReference type="PhylomeDB" id="Q980E3"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043793">
        <property type="term" value="F:beta-ribofuranosylaminobenzene 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004422">
        <property type="entry name" value="RFAP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00144">
        <property type="entry name" value="beta_RFAP_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF6">
        <property type="entry name" value="BETA-RIBOFURANOSYLPHENOL 5'-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004884">
        <property type="entry name" value="Sugar_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="3">
        <location>
            <begin position="196"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR004884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="292" mass="32166" checksum="FECD9777AD27C6A4" modified="2001-10-01" version="1">MIKIVGLSRIHITLFDLEGKYGRLDGGAGVALKYPRIVVRSGNCFKPEVSLPFDVPKICIEEDFEQHIGLGHTTQYLLSVAKLAAEYNFKRLNSYELAKLVKRGGTSGVGVYAFEYGGFIVDGGHSTKVKRELLPSDFSSAPPPPLIARLNFPWYIYVNVVRGGRRVFGKDEINAFKQAKLEGLDTLARVVLMELIPSVAERDLEGALNAISLIQDLGFKKVEVFLQTDEVKKLMNDMVKAGFPAGLSSFGPAVYTFVSSKREGDELISRFGGFVSEPNNEGAKVFWSTMSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q980G9</accession>
    <name>Q980G9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NADH dehydrogenase subunit L (NuoL)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NuoL</name>
        <name evidence="9" type="ordered locus">SSO0328</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90175">
        <property type="entry name" value="H90175"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980G9"/>
    <dbReference type="FunCoup" id="Q980G9">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0328"/>
    <dbReference type="PaxDb" id="273057-SSO0328"/>
    <dbReference type="EnsemblBacteria" id="AAK40663">
        <property type="protein sequence ID" value="AAK40663"/>
        <property type="gene ID" value="SSO0328"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0328"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.323"/>
    <dbReference type="eggNOG" id="arCOG01539">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_277666_0_0_2"/>
    <dbReference type="InParanoid" id="Q980G9"/>
    <dbReference type="PhylomeDB" id="Q980G9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050586">
        <property type="entry name" value="CPA3_Na-H_Antiporter_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01434">
        <property type="entry name" value="NADHDHGNASE5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="398"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="432"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="514"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="543"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="585"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="620"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="745"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="781"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="808"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="848"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="915"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="952"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="991"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1026"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1126"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="7">
        <location>
            <begin position="143"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-Ubiquinone oxidoreductase (complex I) chain 5 N-terminal" evidence="8">
        <location>
            <begin position="583"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="7">
        <location>
            <begin position="686"/>
            <end position="954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK40663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="1148" mass="127464" checksum="8D184872BA9D8664" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q980I7</accession>
    <name>AROC_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chorismate synthase</fullName>
            <shortName evidence="1">CS</shortName>
            <ecNumber evidence="1">4.2.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-enolpyruvylshikimate-3-phosphate phospholyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroC</name>
        <name type="ordered locus">SSO0307</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21020"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="EC" id="4.2.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMNH2</name>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
        </cofactor>
        <text evidence="1">Reduced FMN (FMNH(2)).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the chorismate synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.5" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90173">
        <property type="entry name" value="E90173"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990599.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980I7"/>
    <dbReference type="SMR" id="Q980I7"/>
    <dbReference type="FunCoup" id="Q980I7">
        <property type="interactions" value="167"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0307"/>
    <dbReference type="PaxDb" id="273057-SSO0307"/>
    <dbReference type="EnsemblBacteria" id="AAK40644">
        <property type="protein sequence ID" value="AAK40644"/>
        <property type="gene ID" value="SSO0307"/>
    </dbReference>
    <dbReference type="GeneID" id="1455454"/>
    <dbReference type="GeneID" id="44129280"/>
    <dbReference type="KEGG" id="sso:SSO0307"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.300"/>
    <dbReference type="eggNOG" id="arCOG04133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034547_0_0_2"/>
    <dbReference type="InParanoid" id="Q980I7"/>
    <dbReference type="PhylomeDB" id="Q980I7"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004107">
        <property type="term" value="F:chorismate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07304">
        <property type="entry name" value="Chorismate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.150.10:FF:000002">
        <property type="entry name" value="Chorismate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.150.10">
        <property type="entry name" value="Chorismate synthase AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00300">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000453">
        <property type="entry name" value="Chorismate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035904">
        <property type="entry name" value="Chorismate_synth_AroC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020541">
        <property type="entry name" value="Chorismate_synthase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00033">
        <property type="entry name" value="aroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003793">
        <property type="entry name" value="PRK05382.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085:SF0">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01264">
        <property type="entry name" value="Chorismate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001456">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103263">
        <property type="entry name" value="Chorismate synthase, AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00787">
        <property type="entry name" value="CHORISMATE_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00788">
        <property type="entry name" value="CHORISMATE_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00789">
        <property type="entry name" value="CHORISMATE_SYNTHASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140697" description="Chorismate synthase">
        <location>
            <begin position="1"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="126"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="301"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00300"/>
        </source>
    </evidence>
    <sequence length="391" mass="43179" checksum="CEDE6FC88ADAD5AE" modified="2001-10-01" version="1">MPGNSFGKLFRITTFGESHGPAVGVVIDGVPAGLPLTVEDIKFELEFRRPGRLYVSGRREKDEPEILSGIFNNRTTGSPIAVIVRNTDVISSFYDEIKYKPRPGHADLPFIMKYGYENWDYRGGGRASARETVSRVIAGAVAKKLLMLTDTWIAGHLRSLGPEELSEEVTFEEVLCSKYSPVRASKKDLEEKYEALIKKATQEGDSYGGIAEVIAKNPPIGLGEPVFDKMKAELAKAIMSIPAVMGFEYGLGFIASKMKGSEANDEIIRKNNRIGWKYNYAGGILGGLTNGEDLIVRCAFKPTSSIRKPQKTIDLRNLEESYISVIGRHDPAVAIRGVTVVESMVALTIVDHAMRAGAIPLVKLTEDQANTIQQRWERYVKSCKPMEESQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q980N9</accession>
    <name>Q980N9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0251</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90166">
        <property type="entry name" value="G90166"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990513.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980N9"/>
    <dbReference type="STRING" id="273057.SSO0251"/>
    <dbReference type="PaxDb" id="273057-SSO0251"/>
    <dbReference type="EnsemblBacteria" id="AAK40590">
        <property type="protein sequence ID" value="AAK40590"/>
        <property type="gene ID" value="SSO0251"/>
    </dbReference>
    <dbReference type="GeneID" id="1455400"/>
    <dbReference type="KEGG" id="sso:SSO0251"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.245"/>
    <dbReference type="eggNOG" id="arCOG00993">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127467_0_0_2"/>
    <dbReference type="InParanoid" id="Q980N9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="181" mass="21317" checksum="847BF7703C143039" modified="2001-10-01" version="1">MPFKEGIRMSFSEARIDEVRSKLEVIFSRYNCMNKLSEFICNKSFYELKGKYNQLFMLTNNVDKDRLLEFLSGLLSKGLHPYSIGIPIIIYDMSEILPTLAFGRYLAEICENKLVIFNYNLIYKIIYKKPIYINYAYNYKDVIIVDKMNNFIAYAKVKSKKRGVHEVIPVKDIGWYLRRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q980S1</accession>
    <name>Q980S1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Phosphomannomutase (PmM)</fullName>
            <ecNumber evidence="12">5.4.2.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pmM</name>
        <name evidence="12" type="ordered locus">SSO0207</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="12"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99162">
        <property type="entry name" value="A99162"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990453.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980S1"/>
    <dbReference type="FunCoup" id="Q980S1">
        <property type="interactions" value="277"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0207"/>
    <dbReference type="PaxDb" id="273057-SSO0207"/>
    <dbReference type="EnsemblBacteria" id="AAK40552">
        <property type="protein sequence ID" value="AAK40552"/>
        <property type="gene ID" value="SSO0207"/>
    </dbReference>
    <dbReference type="GeneID" id="1455362"/>
    <dbReference type="KEGG" id="sso:SSO0207"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.206"/>
    <dbReference type="eggNOG" id="arCOG00767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_7_1_2"/>
    <dbReference type="InParanoid" id="Q980S1"/>
    <dbReference type="PhylomeDB" id="Q980S1"/>
    <dbReference type="BRENDA" id="5.4.2.2">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="5.4.2.8">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03087">
        <property type="entry name" value="PGM_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000001">
        <property type="entry name" value="Phosphoglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024086">
        <property type="entry name" value="GlmM_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03990">
        <property type="entry name" value="Arch_GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771:SF1">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="9">
        <location>
            <begin position="3"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="10">
        <location>
            <begin position="154"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="259"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="8">
        <location>
            <begin position="376"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK40552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="455" mass="50265" checksum="E107A9EC192ECCA1" modified="2001-10-01" version="1">MGKLFGTDGIRGVTNTELQPEFALKIGKAIGTYFGKGARILIGRDVRAGGDMLLKAVESGLLSSGVLVYEAGMAPTPAFQYGVKALGYDGGVIITASHNPAEYNGIKVLSPHGIEISREDEDKIEDIYFNSRFHVVEWNGLVNDVKREDKVIETYLRGILSHVDTEKIKSKKYKVLIDPANSVGTLVTPIVARELGCKVFTINGNLDPLFSARTPEPTFESLTESAKVAKQLGVDLAVAHDGDADRAIFIDSMGRVQWGDRSGTLLSYWASVKAPNLPRRIFTAVSSSSLVEEYLKKYNIEVKWTKVGSIDIAHMLFKEKGVAGFEENGGFMYPPHQTVRDGAMSFALMLDMMASENESSAELFNRLPVYYLVKTKVRVTEKSDIKKIYDEVMNKYGNYGNTVTIDGVKIIGSDFWLLVRKSGTEPIIRILVEAKDENKSKELAKELEKLVSELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q980X7</accession>
    <name>Q980X7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD(P)/FAD-dependent oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90154">
        <property type="entry name" value="H90154"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990374.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980X7"/>
    <dbReference type="PaxDb" id="273057-SSO0147"/>
    <dbReference type="EnsemblBacteria" id="AAK40495">
        <property type="protein sequence ID" value="AAK40495"/>
        <property type="gene ID" value="SSO0147"/>
    </dbReference>
    <dbReference type="GeneID" id="1453322"/>
    <dbReference type="KEGG" id="sso:SSO0147"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.145"/>
    <dbReference type="eggNOG" id="arCOG05933">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978674_0_0_2"/>
    <dbReference type="InParanoid" id="Q980X7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="34375" checksum="23B1343975BE8355" modified="2001-10-01" version="1">MIVVIGAGISGLLLSKRVKADLILEEQPVIGGVFASDNVFGKDLPYVLPIINDPAKLPNIEFNYLEYDLKLSHRKYDYFEDKICKYCDKLPTWLNFGLMRKLYVIENITAFIDNLSKNVRIIKEYPIRIDDKKIITNKGNMYKYTRVYNTASLKKMYKLLGVDSSNLKHKAALTLLILTRKTNNKNWNVYLSGDTADSFSVVIKLDNIIKDLDLYYVYSFMDITQKSIDIDRVMLDLKRRQIISLDEIIAFRSKLISEALLFGEINHKITLNMVNCGRLGEWKNYDVIETISRIQNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q981D1</accession>
    <name>Q981D1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1634 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90140">
        <property type="entry name" value="F90140"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922856.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981D1"/>
    <dbReference type="STRING" id="273057.SSO0014"/>
    <dbReference type="PaxDb" id="273057-SSO0014"/>
    <dbReference type="EnsemblBacteria" id="AAK40381">
        <property type="protein sequence ID" value="AAK40381"/>
        <property type="gene ID" value="SSO0014"/>
    </dbReference>
    <dbReference type="GeneID" id="1455272"/>
    <dbReference type="KEGG" id="sso:SSO0014"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.15"/>
    <dbReference type="eggNOG" id="arCOG05396">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140339_1_0_2"/>
    <dbReference type="InParanoid" id="Q981D1"/>
    <dbReference type="PhylomeDB" id="Q981D1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012861">
        <property type="entry name" value="DUF1634"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07843">
        <property type="entry name" value="DUF1634"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="13933" checksum="D0DB868746478F39" modified="2001-10-01" version="1">MDFNNIIGNTLRIGVIISAIIIIFGVALLFVNNGSNGFTITQISSSNSIVNSSIFKPSEIFSGLPKLYGLDYIYLGLMVLIATPVLRVLLGIAQFASERNKLYTIITIIVFFNLMFAIFLLPIIIGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXA3</accession>
    <name>RS19_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein uS19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">30S ribosomal protein S19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps19</name>
        <name evidence="5" type="synonym">rps19AB</name>
        <name type="ordered locus">SSO0715</name>
        <name type="ORF">C10_016</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7 8 9" key="3">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). uS9, uS13 and uS19 stabilize the codon:anticodon duplex (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S small ribosomal subunit, forms a complex with S13 that binds strongly to the 16S ribosomal RNA (PubMed:39753558).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90219">
        <property type="entry name" value="G90219"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991278.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="T=1-140"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="T=1-140"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="T=1-140"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="T=1-140"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="T=1-140"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="T=1-140"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="T=1-140"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="T=1-140"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q9UXA3"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q9UXA3"/>
    <dbReference type="FunCoup" id="Q9UXA3">
        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0715"/>
    <dbReference type="PaxDb" id="273057-SSO0715"/>
    <dbReference type="EnsemblBacteria" id="AAK41014">
        <property type="protein sequence ID" value="AAK41014"/>
        <property type="gene ID" value="SSO0715"/>
    </dbReference>
    <dbReference type="GeneID" id="1454981"/>
    <dbReference type="KEGG" id="sso:SSO0715"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.714"/>
    <dbReference type="eggNOG" id="arCOG04099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097347_1_1_2"/>
    <dbReference type="InParanoid" id="Q9UXA3"/>
    <dbReference type="PhylomeDB" id="Q9UXA3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.860.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_uS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005713">
        <property type="entry name" value="Ribosomal_uS19_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003121">
        <property type="entry name" value="PRK04038.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01025">
        <property type="entry name" value="uS19_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000130015" description="Small ribosomal subunit protein uS19">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="CAB57589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="140" mass="16368" checksum="AC574CAFEDE026EA" modified="2000-05-01" version="1">MSLEIPPEWKNFKYRGKSIDELLNMPMDEFIKLLPSRQRRSLKRGFTDAQRHLLEKVRKYRREGKFNKTIKTHVRNLVILPELIGLKMAVYNGKEFVEFTVTPEMIGHYLGEYSITTKKVEHGEPGLKATRSSLFLAMKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXA5</accession>
    <name>RL2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl2</name>
        <name evidence="1" type="synonym">rpl2Ab</name>
        <name type="ordered locus">SSO0716</name>
        <name type="ORF">C10_014</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90219">
        <property type="entry name" value="H90219"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991279.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXA5"/>
    <dbReference type="SMR" id="Q9UXA5"/>
    <dbReference type="FunCoup" id="Q9UXA5">
        <property type="interactions" value="216"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0716"/>
    <dbReference type="PaxDb" id="273057-SSO0716"/>
    <dbReference type="EnsemblBacteria" id="AAK41015">
        <property type="protein sequence ID" value="AAK41015"/>
        <property type="gene ID" value="SSO0716"/>
    </dbReference>
    <dbReference type="GeneID" id="1454982"/>
    <dbReference type="KEGG" id="sso:SSO0716"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.715"/>
    <dbReference type="eggNOG" id="arCOG04067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036235_0_1_2"/>
    <dbReference type="InParanoid" id="Q9UXA5"/>
    <dbReference type="PhylomeDB" id="Q9UXA5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000001">
        <property type="entry name" value="50S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_A">
        <property type="entry name" value="Ribosomal_uL2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023672">
        <property type="entry name" value="Ribosomal_uL2_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007180">
        <property type="entry name" value="PRK09612.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000129727" description="Large ribosomal subunit protein uL2">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="201"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="223"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="238" mass="25365" checksum="B5CD0765610D0C00" modified="2000-05-01" version="1">MGKNLLQQRAGKGSPTFRNPGWLRIGKVRYPNIFGHFIGKVIDIVHNPGMNAPVALVKLENGTKFLMQAIQGLVVNQKIEFGKGSPIANGNVIEIGDAPEGTIVCNVEENFGDGGKYARSAGSYAVVVGKSGDRVLIRLPSDKIKAVSNKARATVGIVAGGGVIEKPLLKAGANYWKYKVKAKKWPIVRGVAMNVVDHPHGGGLHQSVSRPSTVSRNAPPGRKVGHIAARRTGRKEGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXB9</accession>
    <name>BRIX_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable Brix domain-containing ribosomal biogenesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0731</name>
        <name type="ORF">C20_022</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably involved in the biogenesis of the ribosome.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90221">
        <property type="entry name" value="E90221"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923055.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXB9"/>
    <dbReference type="SMR" id="Q9UXB9"/>
    <dbReference type="STRING" id="273057.SSO0731"/>
    <dbReference type="PaxDb" id="273057-SSO0731"/>
    <dbReference type="EnsemblBacteria" id="AAK41028">
        <property type="protein sequence ID" value="AAK41028"/>
        <property type="gene ID" value="SSO0731"/>
    </dbReference>
    <dbReference type="GeneID" id="1454995"/>
    <dbReference type="KEGG" id="sso:SSO0731"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.728"/>
    <dbReference type="eggNOG" id="arCOG03247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118422_0_0_2"/>
    <dbReference type="InParanoid" id="Q9UXB9"/>
    <dbReference type="PhylomeDB" id="Q9UXB9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10480">
        <property type="entry name" value="Probable brix-domain ribosomal biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00699">
        <property type="entry name" value="BriX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023548">
        <property type="entry name" value="Brix_dom_Rbsml_bgen_prot"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_0000120280" description="Probable Brix domain-containing ribosomal biogenesis protein">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Brix" evidence="1">
        <location>
            <begin position="6"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00699"/>
        </source>
    </evidence>
    <sequence length="170" mass="19614" checksum="63C8F23E576350E2" modified="2000-05-01" version="1">MHIHRTRIVITSSRDPSIRTRNFLNVLTFVLPDSVKITRGKKSKIEIFERAINLGALYLLFVLAKNGNPLRIIVYDLESLSIKYFFKLSGLSLPSDYNVSLNQIKGHSNVCIKLNECDFLRDFLVDMNMYNLTNNCDVVVNSRLIHTNICELLFMLTTKNIKFLKMILEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-02-09" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>P0CD66</accession>
    <accession>O59645</accession>
    <name>AGLU_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Alpha-glucosidase</fullName>
            <ecNumber evidence="2">3.2.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Maltase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">malA</name>
        <name type="ordered locus">SSO3051</name>
        <name type="ORF">C23_036</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Major soluble alpha-glucosidase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Hydrolysis of terminal, non-reducing (1-&gt;4)-linked alpha-D-glucose residues with release of alpha-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.20" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90486">
        <property type="entry name" value="H90486"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988431.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2G3M">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.55 A"/>
        <property type="chains" value="A/B/C/D/E/F=5-693"/>
    </dbReference>
    <dbReference type="PDB" id="2G3N">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.55 A"/>
        <property type="chains" value="A/B/C/D/E/F=5-693"/>
    </dbReference>
    <dbReference type="PDBsum" id="2G3M"/>
    <dbReference type="PDBsum" id="2G3N"/>
    <dbReference type="AlphaFoldDB" id="P0CD66"/>
    <dbReference type="SMR" id="P0CD66"/>
    <dbReference type="FunCoup" id="P0CD66">
        <property type="interactions" value="246"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3051"/>
    <dbReference type="CAZy" id="GH31">
        <property type="family name" value="Glycoside Hydrolase Family 31"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3051"/>
    <dbReference type="EnsemblBacteria" id="AAK43151">
        <property type="protein sequence ID" value="AAK43151"/>
        <property type="gene ID" value="SSO3051"/>
    </dbReference>
    <dbReference type="GeneID" id="1453070"/>
    <dbReference type="GeneID" id="44128770"/>
    <dbReference type="KEGG" id="sso:SSO3051"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3149"/>
    <dbReference type="eggNOG" id="arCOG03663">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000631_7_2_2"/>
    <dbReference type="InParanoid" id="P0CD66"/>
    <dbReference type="PhylomeDB" id="P0CD66"/>
    <dbReference type="BRENDA" id="3.2.1.20">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="P0CD66"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06604">
        <property type="entry name" value="GH31_glucosidase_II_MalA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053497">
        <property type="entry name" value="GH31_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
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</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>P58157</accession>
    <name>KTHY2_SACS2</name>
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        <recommendedName>
            <fullName>Probable thymidylate kinase 2</fullName>
            <ecNumber>2.7.4.9</ecNumber>
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        <alternativeName>
            <fullName>dTMP kinase 2</fullName>
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    <gene>
        <name type="primary">tmk2</name>
        <name type="ordered locus">SSO1192</name>
        <name type="ORF">C50_002</name>
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    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
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    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <reaction>
            <text>dTMP + ATP = dTDP + ADP</text>
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        <text evidence="2">Belongs to the thymidylate kinase family.</text>
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    <keyword id="KW-0067">ATP-binding</keyword>
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    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="213" mass="25001" checksum="3A5BCCFD2F067A7D" modified="2001-06-01" version="1">MRGLLIAFEGIDGSGKSSQAVLLKDWIEMRRDVYLTEWNSSEWIHDIIKEAKKKNMLTSITFSLIHATDFSDRYERYILPMLKSGFVVICDRYVYTAYARDVVRNVDFDWVKRLYSFAIKPNFTFYIRVTPEIALERIRKAKRKIKPQEAGIDILGEIPLEEGFLKYQSRIVEIYDKIAKEESNFITIDGNRPLKDVQIDIRKILGEYIDNSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="149" xmlns="https://uniprot.org/uniprot">
    <accession>P95928</accession>
    <name>APE2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Leucyl aminopeptidase</fullName>
            <ecNumber>3.4.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">ape2</name>
        <name type="synonym">lap</name>
        <name type="ordered locus">SSO2154</name>
        <name type="ORF">C01_030</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1998" name="Mol. Microbiol." volume="29" first="775" last="785">
            <title>A novel aminopeptidase associated with the 60 kDa chaperonin in the thermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Condo I."/>
                <person name="Ruggero D."/>
                <person name="Reinhardt R."/>
                <person name="Londei P."/>
            </authorList>
            <dbReference type="PubMed" id="9723917"/>
            <dbReference type="DOI" id="10.1046/j.1365-2958.1998.00971.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 1-7</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Preferentially acts as a leucyl-aminopeptidase, although it also has activity against other substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.</text>
            <dbReference type="EC" id="3.4.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text>Optimum pH is 6.</text>
        </phDependence>
        <temperatureDependence>
            <text>Optimum temperature is 80 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text>Co-immunoprecipitates with the 60 kDa chaperonin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text>Can be phosphorylated by cell extracts.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.1"/>
    <dbReference type="EMBL" id="AF043465">
        <property type="protein sequence ID" value="AAC63218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y08256">
        <property type="protein sequence ID" value="CAA69432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73098">
        <property type="entry name" value="S73098"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992163.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95928"/>
    <dbReference type="SMR" id="P95928"/>
    <dbReference type="FunCoup" id="P95928">
        <property type="interactions" value="170"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2154"/>
    <dbReference type="MEROPS" id="M01.021"/>
    <dbReference type="PaxDb" id="273057-SSO2154"/>
    <dbReference type="EnsemblBacteria" id="AAK42330">
        <property type="protein sequence ID" value="AAK42330"/>
        <property type="gene ID" value="SSO2154"/>
    </dbReference>
    <dbReference type="GeneID" id="1453657"/>
    <dbReference type="KEGG" id="sso:SSO2154"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2247"/>
    <dbReference type="eggNOG" id="arCOG02969">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003705_0_3_2"/>
    <dbReference type="InParanoid" id="P95928"/>
    <dbReference type="PhylomeDB" id="P95928"/>
    <dbReference type="BRENDA" id="3.4.11.B3">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000012">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000036">
        <property type="entry name" value="Leucyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000006">
        <property type="entry name" value="Puromycin-sensitive aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF174">
        <property type="entry name" value="PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00142">
        <property type="entry name" value="ZINC_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0031">Aminopeptidase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000095107" description="Leucyl aminopeptidase">
        <location>
            <begin position="1"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="238"/>
            <end position="242"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="785" mass="90515" checksum="73ACFDEB1F2832BD" modified="1997-05-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX54</accession>
    <name>Q7LX54_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbon monoxide dehydrogenase, large chain (CutA-3)</fullName>
            <ecNumber evidence="4">1.2.7.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cutA-3</name>
        <name evidence="4" type="ordered locus">SSO2150</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.4" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73093">
        <property type="entry name" value="S73093"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989159.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX54"/>
    <dbReference type="SMR" id="Q7LX54"/>
    <dbReference type="FunCoup" id="Q7LX54">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2150"/>
    <dbReference type="PaxDb" id="273057-SSO2150"/>
    <dbReference type="EnsemblBacteria" id="AAK42326">
        <property type="protein sequence ID" value="AAK42326"/>
        <property type="gene ID" value="SSO2150"/>
    </dbReference>
    <dbReference type="GeneID" id="1453653"/>
    <dbReference type="KEGG" id="sso:SSO2150"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2243"/>
    <dbReference type="eggNOG" id="arCOG01167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_2_0_2"/>
    <dbReference type="InParanoid" id="Q7LX54"/>
    <dbReference type="PhylomeDB" id="Q7LX54"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:anaerobic carbon-monoxide dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.50">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.365.10">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046867">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD2"/>
    </dbReference>
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        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053554">
        <property type="entry name" value="Glyceraldehyde_dh-related"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041018">
        <property type="entry name" value="glyceraldDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908:SF132">
        <property type="entry name" value="ALDEHYDE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="MoCoBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20256">
        <property type="entry name" value="MoCoBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde oxidase/xanthine dehydrogenase a/b hammerhead" evidence="3">
        <location>
            <begin position="17"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="729" mass="80647" checksum="313EC14F2C09D1DB" modified="2011-05-31" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-03-27" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX58</accession>
    <name>Q7LX58_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74056">
        <property type="entry name" value="S74056"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX58"/>
    <dbReference type="PaxDb" id="273057-SSO2144"/>
    <dbReference type="EnsemblBacteria" id="AAK42322">
        <property type="protein sequence ID" value="AAK42322"/>
        <property type="gene ID" value="SSO2144"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2144"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2238"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_8_1_2"/>
    <dbReference type="InParanoid" id="Q7LX58"/>
    <dbReference type="PhylomeDB" id="Q7LX58"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024930">
        <property type="entry name" value="Skp_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111384">
        <property type="entry name" value="OmpH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="131" mass="16055" checksum="56838BB12DBACBE5" modified="2011-05-31" version="1">MPTLGFRFRAYADEQTLRALKAQLKLACKIYNTLRWADIYFYQRDGKGLTQTELRQLALDLRKQDDEYKQLYSQVVQQIADRYYEARQRFFEGLARFPKEKKPHKYYSLVYPQYGWKILQVREIRKRKQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX79</accession>
    <name>Q7LX79_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74029">
        <property type="entry name" value="S74029"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990150.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX79"/>
    <dbReference type="STRING" id="273057.SSO2117"/>
    <dbReference type="PaxDb" id="273057-SSO2117"/>
    <dbReference type="EnsemblBacteria" id="AAK42296">
        <property type="protein sequence ID" value="AAK42296"/>
        <property type="gene ID" value="SSO2117"/>
    </dbReference>
    <dbReference type="GeneID" id="1453623"/>
    <dbReference type="KEGG" id="sso:SSO2117"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2206"/>
    <dbReference type="eggNOG" id="arCOG05371">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090553_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX79"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="274" mass="31075" checksum="A65D3FD1C0931516" modified="2011-05-31" version="1">MKRLFTYIVKQTLSNPNLIGWGVLFVLFWGLLGAFIMAPGFIKQIEPTYIKTIYPNYVAAWYSDLVILSLSSLAVSVSYAIFYQTGTLPYLLRYSKLRADTYIISLYLGFLVVGLVIVLLMTAVIPFMFSNNGIGIITYPSQIHILIPISVLGSLFFISLSTLLTLITIKLKGMRMQNLISFIPLILGYIFYSLFTFSEYPSILDYTSPYLTIVLLLYYGYTGNVPPISVQSQQTVSLEGVIISSVLWTLILTIIDIILMKRIYYTNIEESRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX98</accession>
    <name>Q7LX98_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Maltooligosyltrehalose synthase (TreY)</fullName>
            <ecNumber evidence="2">2.4.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">treY</name>
        <name evidence="2" type="ordered locus">SSO2095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.-" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73089">
        <property type="entry name" value="S73089"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989783.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX98"/>
    <dbReference type="SMR" id="Q7LX98"/>
    <dbReference type="FunCoup" id="Q7LX98">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2095"/>
    <dbReference type="CAZy" id="GH13">
        <property type="family name" value="Glycoside Hydrolase Family 13"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2095"/>
    <dbReference type="EnsemblBacteria" id="AAK42274">
        <property type="protein sequence ID" value="AAK42274"/>
        <property type="gene ID" value="SSO2095"/>
    </dbReference>
    <dbReference type="GeneID" id="1453601"/>
    <dbReference type="KEGG" id="sso:SSO2095"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2173"/>
    <dbReference type="eggNOG" id="arCOG02955">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005045_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LX98"/>
    <dbReference type="PhylomeDB" id="Q7LX98"/>
    <dbReference type="BRENDA" id="5.4.99.15">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047470">
        <property type="term" value="F:(1,4)-alpha-D-glucan 1-alpha-D-glucosylmutase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030980">
        <property type="term" value="P:alpha-glucan catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11336">
        <property type="entry name" value="AmyAc_MTSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000342">
        <property type="entry name" value="Malto-oligosyltrehalose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000287">
        <property type="entry name" value="Maltooligosyl trehalose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1590.10">
        <property type="entry name" value="Maltooligosyl trehalose synthase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.200">
        <property type="entry name" value="Maltooligosyl trehalose synthase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012767">
        <property type="entry name" value="Trehalose_TreY"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011084">
        <property type="entry name" value="PRK14511.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF216">
        <property type="entry name" value="MALTOOLIGOSYL TREHALOSE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="1">
        <location>
            <begin position="15"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="732" mass="86609" checksum="8BD55028830054E9" modified="2011-05-31" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXG1</accession>
    <name>Q7LXG1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0816</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99232">
        <property type="entry name" value="A99232"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXG1"/>
    <dbReference type="PaxDb" id="273057-SSO0816"/>
    <dbReference type="DNASU" id="1455079"/>
    <dbReference type="EnsemblBacteria" id="AAK41112">
        <property type="protein sequence ID" value="AAK41112"/>
        <property type="gene ID" value="SSO0816"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0816"/>
    <dbReference type="HOGENOM" id="CLU_2271158_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXG1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="110" mass="12558" checksum="7152408D7356439B" modified="2011-05-31" version="1">MNGFSSLLTCWKNYVAIIFASTLLSLLFSFLNLLISASILTLFYLIDLIVPPFYPANVMEKPIVFLSPILIISITIYILVKLFFSNLIFFISLIILFILDVILSFKRAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXN2</accession>
    <name>Q7LXN2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Diphthamide synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90215">
        <property type="entry name" value="E90215"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991226.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXN2"/>
    <dbReference type="SMR" id="Q7LXN2"/>
    <dbReference type="FunCoup" id="Q7LXN2">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0678"/>
    <dbReference type="PaxDb" id="273057-SSO0678"/>
    <dbReference type="EnsemblBacteria" id="AAK40980">
        <property type="protein sequence ID" value="AAK40980"/>
        <property type="gene ID" value="SSO0678"/>
    </dbReference>
    <dbReference type="GeneID" id="1454947"/>
    <dbReference type="KEGG" id="sso:SSO0678"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.679"/>
    <dbReference type="eggNOG" id="arCOG00035">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010289_0_2_2"/>
    <dbReference type="InParanoid" id="Q7LXN2"/>
    <dbReference type="PhylomeDB" id="Q7LXN2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="AANH_PF0828-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000145">
        <property type="entry name" value="ATP-binding domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1490.10">
        <property type="entry name" value="putative n-type atp pyrophosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022427">
        <property type="entry name" value="MJ0570_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03679">
        <property type="entry name" value="arCOG00187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196:SF2">
        <property type="entry name" value="DIPHTHINE--AMMONIA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039123">
        <property type="entry name" value="Diphthamide_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthamide synthase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="222" mass="25574" checksum="C67F5F29712CE82F" modified="2011-05-31" version="1">MKICVLYSGGKDSTYALHWAVFKGFEVVCLITLIPKREDSWMFQYPNVIYTKYQAEAMDFKLITFGTSGEKDKELEDLKKALLQAKNEGADGIVSGALLSDYQRLNISIIAEELGLKTYTPLWRKSQEEYMRWLVKEGFKFIITSASAYGFPFDLVGKEITTEDVEKIIERARRYGFNPAFEGGEAETFVTYAPLFKTQLRVNGKLKRISEYECRYEITDIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXP3</accession>
    <name>Q7LXP3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Threonylcarbamoyl-AMP synthase</fullName>
            <shortName evidence="12">TC-AMP synthase</shortName>
            <ecNumber evidence="3 12">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">SSO0664</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>L-threonine + hydrogencarbonate + ATP = L-threonylcarbamoyladenylate + diphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the SUA5 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="3 12"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99214">
        <property type="entry name" value="A99214"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991206.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXP3"/>
    <dbReference type="FunCoup" id="Q7LXP3">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0664"/>
    <dbReference type="PaxDb" id="273057-SSO0664"/>
    <dbReference type="EnsemblBacteria" id="AAK40968">
        <property type="protein sequence ID" value="AAK40968"/>
        <property type="gene ID" value="SSO0664"/>
    </dbReference>
    <dbReference type="GeneID" id="1454935"/>
    <dbReference type="KEGG" id="sso:SSO0664"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.665"/>
    <dbReference type="eggNOG" id="arCOG01952">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031397_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXP3"/>
    <dbReference type="PhylomeDB" id="Q7LXP3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11030:FF:000001">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.870.10:FF:000008">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11030">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038385">
        <property type="entry name" value="Sua5/YwlC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005145">
        <property type="entry name" value="Sua5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010923">
        <property type="entry name" value="T(6)A37_SUA5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050156">
        <property type="entry name" value="TC-AMP_synthase_SUA5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00057">
        <property type="entry name" value="L-threonylcarbamoyladenylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490:SF16">
        <property type="entry name" value="THREONYLCARBAMOYL-AMP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03481">
        <property type="entry name" value="Sua5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004930">
        <property type="entry name" value="Tln_factor_SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="14">
        <location>
            <begin position="14"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="13">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    <feature type="binding site" evidence="13">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00015492"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR004930-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAK40968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="358" mass="39394" checksum="CC4E15E1E18FCE4E" modified="2011-05-31" version="1">MTEVLKVDPLYPELDKIKKAANVIKAGGTVVFPTETVYGLGANALDPEAVKKIFIAKNRPMDNPLIVHISNIDQLYEVAKDIPKKIINLVNIVWPGPLTFVLKKTEKVPKETTGGLETVAVRMPAHPVALALINESGVPIAAPSANLATKPSPTRVEHVIDDIYGKVDVILDSGETFFGVESTVINVTVDPPVLLRPGPFSVEELERLFGKVVVPEQAKGTAEFDVALAPGMKYKHYAPTKRLLVVENRSVLRGVINELQKRSCRIALLCASEVCKQFRGEKIEIIELGSENNLYEISRNLFDSFRKLDKSNVDIGVIHSISEKGIGLAIMNRTRKASGFSSIYNIEDVWKYVGNKTQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2019-01-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LYJ6</accession>
    <name>RN081_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR system ring nuclease SSO2081</fullName>
            <ecNumber evidence="4">4.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2081</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2018" name="Nature" volume="562" first="277" last="280">
            <title>Ring nucleases deactivate type III CRISPR ribonucleases by degrading cyclic oligoadenylate.</title>
            <authorList>
                <person name="Athukoralage J.S."/>
                <person name="Rouillon C."/>
                <person name="Graham S."/>
                <person name="Grueschow S."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="30232454"/>
            <dbReference type="DOI" id="10.1038/s41586-018-0557-5"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>COFACTOR</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>MUTAGENESIS OF SER-11 AND 105-ARG-LYS-106</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 4">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). A nuclease that degrades cyclic oligoadenylates (cOA), second messengers that induce an antiviral state important for defense against invading nucleic acids. Destruction of cOA deactivates the Csx1 ribonuclease, preventing uncontrolled degradation of cellular RNA. Degrades cA4 (a tetraadenylate ring) into a linear diadenylate product with 5'-OH and 2',3'-cyclic phosphate termini. Is 10-fold more active than SSO1393, suggesting this is the major cA4 degradation enzyme. Is highly specific for cA4; it has very poor activity on cA6 and no discernible activity against a number of cyclic dinucleotides. There may be 2 active sites per homodimer (PubMed:30232454).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>cyclic tetraadenylate = 2 5'-hydroxy-diadenylate 2',3'-cylic phosphate</text>
            <dbReference type="Rhea" id="RHEA:58012"/>
            <dbReference type="ChEBI" id="CHEBI:142457"/>
            <dbReference type="ChEBI" id="CHEBI:142458"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <text evidence="2">Does not require a metal cofactor.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <text evidence="2">kcat is 0.23 min(-1).</text>
        </kinetics>
    </comment>
    <comment type="subunit">
        <text evidence="1 5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cOA ring nuclease family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.-" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73092">
        <property type="entry name" value="S73092"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989802.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="7YGH">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.11 A"/>
        <property type="chains" value="A/B=1-178"/>
    </dbReference>
    <dbReference type="PDB" id="7YGL">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B=1-178"/>
    </dbReference>
    <dbReference type="PDB" id="7YHL">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B=1-178"/>
    </dbReference>
    <dbReference type="PDB" id="8HTW">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B=1-178"/>
    </dbReference>
    <dbReference type="PDBsum" id="7YGH"/>
    <dbReference type="PDBsum" id="7YGL"/>
    <dbReference type="PDBsum" id="7YHL"/>
    <dbReference type="PDBsum" id="8HTW"/>
    <dbReference type="AlphaFoldDB" id="Q7LYJ6"/>
    <dbReference type="SMR" id="Q7LYJ6"/>
    <dbReference type="STRING" id="273057.SSO2081"/>
    <dbReference type="PaxDb" id="273057-SSO2081"/>
    <dbReference type="DNASU" id="1453589"/>
    <dbReference type="EnsemblBacteria" id="AAK42262">
        <property type="protein sequence ID" value="AAK42262"/>
        <property type="gene ID" value="SSO2081"/>
    </dbReference>
    <dbReference type="GeneID" id="1453589"/>
    <dbReference type="KEGG" id="sso:SSO2081"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2159"/>
    <dbReference type="eggNOG" id="arCOG03847">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514679_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LYJ6"/>
    <dbReference type="PhylomeDB" id="Q7LYJ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10770">
        <property type="entry name" value="Hypothetical protein VC1899 like domain (Restriction endonuclease-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019092">
        <property type="entry name" value="SSO2081-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040581">
        <property type="entry name" value="cas_Crn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09623">
        <property type="entry name" value="Cas_NE0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000446011" description="CRISPR system ring nuclease SSO2081">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Transition state stabilizer" evidence="5">
        <location>
            <begin position="105"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="3.5-fold decrease in kcat for degradation of cA4." evidence="2">
        <original>S</original>
        <variation>A</variation>
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No degradation of cA4." evidence="2">
        <original>RK</original>
        <variation>AA</variation>
        <location>
            <begin position="105"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="2"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="turn" evidence="10">
        <location>
            <begin position="57"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="65"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="98"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="turn" evidence="10">
        <location>
            <begin position="118"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="122"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="130"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="152"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="157"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="172"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q97YD2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="30232454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="30232454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="30232454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="7YGH"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="7YHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="8HTW"/>
        </source>
    </evidence>
    <sequence length="178" mass="20213" checksum="B0078636F21B2A03" modified="2011-05-31" version="1">MVKLVATLGTSPGGVIESFLYLVKKGENIDEVRVVTTSNAEVKKAWRIVRLMFVCCIQEKFPKVEISEHPLDIEDIYSEDDLRKVREFVEKQLGEGDYLDITGGRKSMSVAAALAAKNKGVKIITSIIPQDDYNKISKKVRELKEIPEIKNRGECRQEMKETYCSLIVQDARSIEFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TU8</accession>
    <name>Q97TU8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISC1234</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2882</name>
        <name evidence="3" type="ordered locus">SSO3028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90466">
        <property type="entry name" value="E90466"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924011.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TU8"/>
    <dbReference type="STRING" id="273057.SSO2882"/>
    <dbReference type="PaxDb" id="273057-SSO2882"/>
    <dbReference type="EnsemblBacteria" id="AAK42988">
        <property type="protein sequence ID" value="AAK42988"/>
        <property type="gene ID" value="SSO2882"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK43129">
        <property type="protein sequence ID" value="AAK43129"/>
        <property type="gene ID" value="SSO3028"/>
    </dbReference>
    <dbReference type="GeneID" id="1453048"/>
    <dbReference type="KEGG" id="sso:SSO2882"/>
    <dbReference type="KEGG" id="sso:SSO3028"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2968"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075479_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="319" mass="36790" checksum="D7157B8539A1BF9A" modified="2001-10-01" version="1">MGVEGSTMARTLRHIPNLMYYPLPPIEDMPWREKWLTEIKPVLDTMDLERVLGEGALVYLKLLIVMVLYSCSYRDAVKMVNVNVVVAWFVGRKVGKSTLHDFVGRLYGVRKKLLEISFKLEEKCLPSYLPASAHLVDFMAWLVDSFLLDLPPGKRSVETFREKAELERREGNLERARKLLSLGRTKRRFEGRWTKKRGVSHYGLKAVAVISVSLFVRSITVKPANFSDKRFKSPLKGIKIADRGFSPSPTQLIAREKPFTTLRAHVEFFGTYLNAFWRPYGTTTWRNDVFLHVLGVIYNIKMFLAIQRRTPPGRRAVQL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-01-23" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U19</accession>
    <name>Y3206_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>RutC family protein SSO3206</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO3206</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90505">
        <property type="entry name" value="H90505"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991672.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U19"/>
    <dbReference type="SMR" id="Q97U19"/>
    <dbReference type="FunCoup" id="Q97U19">
        <property type="interactions" value="231"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3206"/>
    <dbReference type="PaxDb" id="273057-SSO3206"/>
    <dbReference type="EnsemblBacteria" id="AAK43303">
        <property type="protein sequence ID" value="AAK43303"/>
        <property type="gene ID" value="SSO3206"/>
    </dbReference>
    <dbReference type="GeneID" id="1453222"/>
    <dbReference type="KEGG" id="sso:SSO3206"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3308"/>
    <dbReference type="eggNOG" id="arCOG01630">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100715_7_3_2"/>
    <dbReference type="InParanoid" id="Q97U19"/>
    <dbReference type="PhylomeDB" id="Q97U19"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.40:FF:000001">
        <property type="entry name" value="L-PSP family endoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006056">
        <property type="entry name" value="RidA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019897">
        <property type="entry name" value="RidA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00004">
        <property type="entry name" value="Rid family detoxifying hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF39">
        <property type="entry name" value="2-IMINOBUTANOATE_2-IMINOPROPANOATE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01094">
        <property type="entry name" value="UPF0076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000170345" description="RutC family protein SSO3206">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="126" mass="14250" checksum="A2685038EAC59502" modified="2001-10-01" version="1">MKEIIFTEKAPKPIGPYSQGVKVGDILYVSGQIPVDPKTNEVVGKNIEEQTIRVIENIKAVLEAAGYMLDDVVMSFVYLKDIKDFQRFNEVYSKYFSNKPPARVTVEVSRLPRDVLIEITVIAQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U52</accession>
    <name>Q97U52_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">First ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO3171</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90501">
        <property type="entry name" value="G90501"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924154.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U52"/>
    <dbReference type="STRING" id="273057.SSO3171"/>
    <dbReference type="PaxDb" id="273057-SSO3171"/>
    <dbReference type="EnsemblBacteria" id="AAK43270">
        <property type="protein sequence ID" value="AAK43270"/>
        <property type="gene ID" value="SSO3171"/>
    </dbReference>
    <dbReference type="GeneID" id="1453189"/>
    <dbReference type="KEGG" id="sso:SSO3171"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3275"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="InParanoid" id="Q97U52"/>
    <dbReference type="PhylomeDB" id="Q97U52"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04762">
        <property type="entry name" value="HTH_MerR-trunc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1660.10:FF:000013">
        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2170:FF:000002">
        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1390:FF:000002">
        <property type="entry name" value="ORF1 in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="5">
        <location>
            <begin position="55"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="192" mass="22499" checksum="A1533729EABDA4B2" modified="2001-10-01" version="1">MLRPKEACQRLGISYATLREYVKKGYIKPVILQSGKWRFREEDVERLMGIIRKRKVILYARVSSSTQKDELVNQVKYLEEQVKEYDLVITDIGSGLNMKRKGFLKLLRMILNNEVSRVVVAYPDRLVRFGFEILEETCKAHNCEIVVLNQEDKEEELVEDLMSVLVSFSGKLYGMRSHEYEKVKKCAEELKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U84</accession>
    <name>Q97U84_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90497">
        <property type="entry name" value="F90497"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U84"/>
    <dbReference type="STRING" id="273057.SSO3139"/>
    <dbReference type="PaxDb" id="273057-SSO3139"/>
    <dbReference type="EnsemblBacteria" id="AAK43237">
        <property type="protein sequence ID" value="AAK43237"/>
        <property type="gene ID" value="SSO3139"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3139"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3243"/>
    <dbReference type="eggNOG" id="arCOG06002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_583459_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U84"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="478" mass="53578" checksum="C936350594CA78E0" modified="2001-10-01" version="1">MGILWRTLPRWGVMIMNKGLSNVISVIILIFIILLVLVPMLYYLEYSSQYNAVTQSVVNNYVYLKNLQISQVTTGHPSLYYNGSSIFLIYSNGTFVPPSNLTIVSILYLNSNGIWVNVTSIKYPLVVSQGQLINLPSYTSGKPIIIVTSLGNIFFLQPGSSIGPFATASKGGVEILAQIYTKNGPLSVSANITSNIYGSLKNYTTPVAFPNQTGTFTVKAPQYVYYQNSKGQIVTGVFYNWIKLGSGTLNSTTTQGVKVTLQGQPLVLIANYSVITTTVNLKICTNDPNVPITVNIDGSNYTITRSYTVQVPAGYVNITLYTLQFNDTTQQQQGIIKHYIYSNSTYNGNVYTARSFILFIPPSSSQTPVVYIKYYNNYNYYEIYIYGYKLPGSVNLILNDTVYNYNKYYWIIGGNYSFSPTGIVNIPYSYGAQTVTFQYSNGTYFEYIFPNIPSYVIINQPMNIEVYYDIIIYWTSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UB5</accession>
    <name>Q97UB5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH iclR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO3103</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90493">
        <property type="entry name" value="G90493"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UB5"/>
    <dbReference type="PaxDb" id="273057-SSO3103"/>
    <dbReference type="EnsemblBacteria" id="AAK43206">
        <property type="protein sequence ID" value="AAK43206"/>
        <property type="gene ID" value="SSO3103"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3103"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3210"/>
    <dbReference type="eggNOG" id="arCOG00393">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154365_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UB5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="HTH iclR-type" evidence="2">
        <location>
            <begin position="207"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="262" mass="29517" checksum="ED95A7E46ED4590C" modified="2001-10-01" version="1">MMKILLLLLLPLITVPVIISHSSILNIYYNGTVEAELTGNVNQFVLIGHNITNLKIIGAKYNLSGNTLYLTPSNTTVFIYYNAILPRGVIQINEPYNATINIYLPLNSSINYISPQPYSFNVMSDYYNVTFANVNSVIWLYTLSSHVTTAQNELEIPMIIGGLISSDAVLVSLIIFLLRRGNVRVLKEEESVDFVPEVLDERDTIVLEAIKMGTNTLADIVRQTGLPKSTAYRRVKKLVKLGYIEEIREEGKIRYVVRSDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UB7</accession>
    <name>Q97UB7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO3100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90493">
        <property type="entry name" value="E90493"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UB7"/>
    <dbReference type="PaxDb" id="273057-SSO3100"/>
    <dbReference type="EnsemblBacteria" id="AAK43204">
        <property type="protein sequence ID" value="AAK43204"/>
        <property type="gene ID" value="SSO3100"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3100"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3206"/>
    <dbReference type="eggNOG" id="arCOG07269">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1149850_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UB7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="247" mass="27524" checksum="8703DBF4E97F28F6" modified="2001-10-01" version="1">MKRFMILGNSCSSCRLLNLLYFDVNASSKVYCFTVSWNQGLYGIFNQLQIVDRQTLLVGLVIITLIVNVILGVELYYTLHNRPTQPVGGIPINVSTVQIISPSSSTTTNTTSGATSTITTTQSSTTNSGSSTTTKQPQTYSFSFKAEFKIHVKEHGIYIVGIKPNITFSQLYVILYFEDGQVVTLNLNQTYVNVTIKENDVKVTAYIYGKSYENLTPQQILNDIGLYFKFIGNSTNSTVMKTQMSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UE1</accession>
    <name>Q97UE1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO11915</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90490">
        <property type="entry name" value="E90490"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924124.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UE1"/>
    <dbReference type="STRING" id="273057.SSO11915"/>
    <dbReference type="PaxDb" id="273057-SSO11915"/>
    <dbReference type="EnsemblBacteria" id="AAK43180">
        <property type="protein sequence ID" value="AAK43180"/>
        <property type="gene ID" value="SSO11915"/>
    </dbReference>
    <dbReference type="GeneID" id="44089189"/>
    <dbReference type="KEGG" id="sso:SSO11915"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3182"/>
    <dbReference type="eggNOG" id="arCOG09928">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3178768_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UE1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="46" mass="5414" checksum="65D10644E52F7A2F" modified="2001-10-01" version="1">MKSLSTERDTLIKVDKAFPWKIFRDKLESLYSKKPGRTSFYPSKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UQ3</accession>
    <name>Q97UQ3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2953</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90474">
        <property type="entry name" value="H90474"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924052.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UQ3"/>
    <dbReference type="PaxDb" id="273057-SSO2953"/>
    <dbReference type="EnsemblBacteria" id="AAK43055">
        <property type="protein sequence ID" value="AAK43055"/>
        <property type="gene ID" value="SSO2953"/>
    </dbReference>
    <dbReference type="GeneID" id="1452974"/>
    <dbReference type="KEGG" id="sso:SSO2953"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3042"/>
    <dbReference type="eggNOG" id="arCOG07238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099722_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UQ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="193" mass="22777" checksum="2847891574E9AE02" modified="2001-10-01" version="1">MLVYVFWHQRAKEFPAKEYEGSLLNFHDYFNKKAKVEGYLGSLVVKVDHVPWIEGEVYEDWYFMESSKTLDLLNNIIIESNDIKTVHDSIAKMARNGKGGLYRLLNGEPSSPSYRYGYWISKPIGMRYEDFYTEIKPFSQNLWRRQLAMGPLSEFCIFSNEYFKVSAKYSPIYQQRILLYSNDKEKTNLAPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UW2</accession>
    <name>Q97UW2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90466">
        <property type="entry name" value="C90466"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UW2"/>
    <dbReference type="STRING" id="273057.SSO2881"/>
    <dbReference type="PaxDb" id="273057-SSO2881"/>
    <dbReference type="EnsemblBacteria" id="AAK42986">
        <property type="protein sequence ID" value="AAK42986"/>
        <property type="gene ID" value="SSO2881"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2881"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2965"/>
    <dbReference type="eggNOG" id="arCOG01357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032214_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UW2"/>
    <dbReference type="PhylomeDB" id="Q97UW2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731:SF4">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731">
        <property type="entry name" value="SLL1084 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="176"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="502" mass="57210" checksum="A0968A987BA2F31A" modified="2001-10-01" version="1">MVPRFFMDRIFAPPMSTDKEGKAIVAPYPLRKVEAVLAKSGFNVVVTTPEKLEKVVTSKGTKVIGINVHDPRGIEPVSAKLSMIFGGGETWTAKFFDELGEKVSKLKEKFHLKVVVGGAGAWQLEKDPPRWADVIFLGHAEVDFPEVVKKLEQGEEVPRVVRGRYPRKLDEIPPIINPSRGGEVQITRGCPRGCWFCSVTPDTFISFPLDYIMREVEVNMKAGIKDVSLITDDMLLYGAKRLNEVNHDAIVRLYAELKRAGVDYINFAHISAAPVKLSPKTVKAMAEIAEWNEEKAVSPVVGLETGSEKIFNKYMKMKAFPWNYTNWKDLIVESTAIMNESYIFPCYTMTIGYPEETDKDVEDSIKLVEYIIDHEPIAWIFPLPVIPMGLSKIRDNPLPALERLPSRYWDLLYLSWKYDLKITRRLGNTIAYTSKNPFIRSIVTYMIDKVFKTIEWYFERLKETKGKSALEYRELNLNTTYGVVWSLFQLLRLAFSSSLPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UW4</accession>
    <name>Q97UW4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">XdhC Rossmann domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2879</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90466">
        <property type="entry name" value="A90466"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989820.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UW4"/>
    <dbReference type="SMR" id="Q97UW4"/>
    <dbReference type="STRING" id="273057.SSO2879"/>
    <dbReference type="PaxDb" id="273057-SSO2879"/>
    <dbReference type="EnsemblBacteria" id="AAK42984">
        <property type="protein sequence ID" value="AAK42984"/>
        <property type="gene ID" value="SSO2879"/>
    </dbReference>
    <dbReference type="GeneID" id="1452903"/>
    <dbReference type="KEGG" id="sso:SSO2879"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2963"/>
    <dbReference type="eggNOG" id="arCOG01929">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041115_3_1_2"/>
    <dbReference type="InParanoid" id="Q97UW4"/>
    <dbReference type="PhylomeDB" id="Q97UW4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052698">
        <property type="entry name" value="MoCofactor_Util/Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027051">
        <property type="entry name" value="XdhC_Rossmann_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30388">
        <property type="entry name" value="ALDEHYDE OXIDOREDUCTASE MOLYBDENUM COFACTOR ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30388:SF6">
        <property type="entry name" value="XANTHINE DEHYDROGENASE SUBUNIT A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13478">
        <property type="entry name" value="XdhC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XdhC Rossmann" evidence="1">
        <location>
            <begin position="84"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="267" mass="29237" checksum="30C0CF62DD7893B2" modified="2001-10-01" version="1">MSSCEIFPLISKLSAEGKRFAIVSIYKGNRLERTIVSDGRVLLGSLDRNIIEIALEALEKGKVIQKDLEDGKLVIEPIEPRPAIIVVGSGIIARFLAKLAIDMGYYVAVVGSGDVKEEDFSGVYAISNDLNLLEQMVSEDSIVIVANEGGKPYDIDALYIAMKKRARFVGLLASQKRAALMISLLVKRDIPLEEIKRRLHSPLGLDIGSKTAEEIALSILSEVVLDLRGGTGKRLQEVKNPYLLLDDALAGKIEDKCMFVPKSLSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UX0</accession>
    <name>Q97UX0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3-hydroxyacyl-CoA dehydrogenase (Hdb-2)</fullName>
            <ecNumber evidence="4">1.1.1.157</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hdb-2</name>
        <name evidence="4" type="ordered locus">SSO2871</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.157" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90465">
        <property type="entry name" value="C90465"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989828.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UX0"/>
    <dbReference type="FunCoup" id="Q97UX0">
        <property type="interactions" value="293"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2871"/>
    <dbReference type="PaxDb" id="273057-SSO2871"/>
    <dbReference type="EnsemblBacteria" id="AAK42978">
        <property type="protein sequence ID" value="AAK42978"/>
        <property type="gene ID" value="SSO2871"/>
    </dbReference>
    <dbReference type="GeneID" id="1452897"/>
    <dbReference type="KEGG" id="sso:SSO2871"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2957"/>
    <dbReference type="eggNOG" id="arCOG00249">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_2_0_2"/>
    <dbReference type="InParanoid" id="Q97UX0"/>
    <dbReference type="PhylomeDB" id="Q97UX0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008691">
        <property type="term" value="F:3-hydroxybutyryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000009">
        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075:SF5">
        <property type="entry name" value="3-HYDROXYBUTYRYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="3">
        <location>
            <begin position="5"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="2">
        <location>
            <begin position="186"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="2">
        <location>
            <begin position="303"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="370" mass="41478" checksum="261A16AD5A9F46CB" modified="2001-10-01" version="1">MIRNVLVVGAGTMGHGIAEVSAIAGYNVYLSDVSKDILNNAIQKVKWSLEKLYEKGNLKESVEDVLGRIKTIEGLSNELREIDISIEAIPEKLDLKRQLFSKLEELLPNNAILATNTSSLPITEIASAVKRQERVIGTHFFNPPVLMQLVEIIRGSKTSDDTVKQTYEFIKSLRKIPIMVNKDVPGFVVNRILLRIITTACMLVEKGICEYTAIDAVARYKLGFPMGIFELVDYTGVDVNYYVSSAMMERGFKAYPCKTLEEMTKKGDLGVKSGKGFYTYPSNKYIKPNLPPQLAEKINPLYIIAPAISEANWMVKNGIASKEDIDLGVRLGLSFPKGIFDYEREFGRESVTKTLEDLRRISGMEEYLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UX2</accession>
    <name>Q97UX2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NAD-dependent malic enzyme (Malate oxidoreductase)</fullName>
            <ecNumber evidence="5">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2869</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90465">
        <property type="entry name" value="A90465"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UX2"/>
    <dbReference type="FunCoup" id="Q97UX2">
        <property type="interactions" value="249"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2869"/>
    <dbReference type="PaxDb" id="273057-SSO2869"/>
    <dbReference type="EnsemblBacteria" id="AAK42976">
        <property type="protein sequence ID" value="AAK42976"/>
        <property type="gene ID" value="SSO2869"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2869"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2955"/>
    <dbReference type="eggNOG" id="arCOG00853">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034446_2_1_2"/>
    <dbReference type="InParanoid" id="Q97UX2"/>
    <dbReference type="PhylomeDB" id="Q97UX2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004473">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NADP+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05311">
        <property type="entry name" value="NAD_bind_2_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051674">
        <property type="entry name" value="Malate_Decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045213">
        <property type="entry name" value="Malic_NAD-bd_bact_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43237">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43237:SF4">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="4">
        <location>
            <begin position="17"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="3">
        <location>
            <begin position="162"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="432" mass="47940" checksum="D3D8B4F22C75110B" modified="2001-10-01" version="1">MIYFFMVDAVELSRKYEGKIEIYPKVPITSYNDFALIYTPGVAEVSKAIYKDPDKSFELTSRWNTIAVVTDGTRVLGLGNIGPTAAMPVMEGKSLLFKYLGGVDAIPLPINVKSADEFTNVVKALETSFGGINLEDIESPKCFYILEKLQSMLNIPVWHDDQQGTAAAILAGLINAMILTNKDPKESKVVFYGAGASNIAAARILAKYGFKYENIVMIDSKGPLYADREDVDHLMLHHKWKYELAIKTNKWEAKEIKDAFSGADAVVAASTPGPNVIKKEWISLMNKEAIVFALANPVPEIWPQDAKEAGAKIVATGRSDFPNQVNNSLIFPAIFRGVLDSRAKAITDEMTIAASEALAKYARDRGINENYIIPRMDEWEAFYEEAAAVASKAVELGLARVKKSREEFREIAKERIIRARKIMNLVISTWSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UX3</accession>
    <name>Q97UX3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2868</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90464">
        <property type="entry name" value="H90464"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989833.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UX3"/>
    <dbReference type="FunCoup" id="Q97UX3">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2868"/>
    <dbReference type="PaxDb" id="273057-SSO2868"/>
    <dbReference type="EnsemblBacteria" id="AAK42975">
        <property type="protein sequence ID" value="AAK42975"/>
        <property type="gene ID" value="SSO2868"/>
    </dbReference>
    <dbReference type="GeneID" id="1452894"/>
    <dbReference type="KEGG" id="sso:SSO2868"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2954"/>
    <dbReference type="eggNOG" id="arCOG00271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978689_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UX3"/>
    <dbReference type="PhylomeDB" id="Q97UX3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050638">
        <property type="entry name" value="AA-Vitamin_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322:SF18">
        <property type="entry name" value="S-ADENOSYLMETHIONINE_S-ADENOSYLHOMOCYSTEINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="10"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="137"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="274" mass="30723" checksum="44EF99B47071EA78" modified="2001-10-01" version="1">MKILKYLLPYVTITSFNYYFAKDAITFSSPIFFNLIRYVISSIIFLSISRGKILFNRDVIQLAVYTTVSSLLWAYGLLYVSPAESAVLSYTMPLFSIPISFIILLERPAVLEIVGVVIGFSGVILYGLPLVHGLTLFGSIITIVNALFWGLFTVFYRKLKGYDPITVNASQFSIGSIILALLLPIHHNIDPNPEFYIGIAYTSTLGGAISFFLWNMMVKAEKIPKVTVLSFSVPILTTLVEFLLYHVLPFPIQLFGIFLMFLGILISRSRTLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UZ6</accession>
    <name>Q97UZ6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90461">
        <property type="entry name" value="H90461"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UZ6"/>
    <dbReference type="STRING" id="273057.SSO2843"/>
    <dbReference type="PaxDb" id="273057-SSO2843"/>
    <dbReference type="EnsemblBacteria" id="AAK42951">
        <property type="protein sequence ID" value="AAK42951"/>
        <property type="gene ID" value="SSO2843"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2843"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2927"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850727_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UZ6"/>
    <dbReference type="PhylomeDB" id="Q97UZ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="138" mass="15733" checksum="36285E1238535855" modified="2001-10-01" version="1">MPKGCKVAIESTGVYHINLAKYLGKEYDVRIINPLILKKFKDFGDKKSDKNDKKLAELVVSMGSKLTTSEAKELTSQWDFITRSIARVKNRLRRDLILLGYKDSLSGKNLNEVLRGEDNIVLSEVSFWVSWRGLRLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V42</accession>
    <name>Q97V42_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2299 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90455">
        <property type="entry name" value="H90455"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V42"/>
    <dbReference type="STRING" id="273057.SSO2790"/>
    <dbReference type="PaxDb" id="273057-SSO2790"/>
    <dbReference type="EnsemblBacteria" id="AAK42903">
        <property type="protein sequence ID" value="AAK42903"/>
        <property type="gene ID" value="SSO2790"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2790"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2876"/>
    <dbReference type="eggNOG" id="arCOG01714">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137738_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V42"/>
    <dbReference type="PhylomeDB" id="Q97V42"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd17510">
        <property type="entry name" value="T3SC_YbjN-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018747">
        <property type="entry name" value="DUF2299"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10061">
        <property type="entry name" value="DUF2299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="16019" checksum="6AB39576245AF7C6" modified="2001-10-01" version="1">MSVMVSDSEVFNWFKELGMRVEQVKSGDLYFHFTVAPPMGGLPVSVIRPRPDSTYYIVAVLLELDQERLRNNKRLVEQIKRELLRMNVEFFFTPNDKEPKSIQVARLMFTDGLTKNEALNNVTLVKNSALLVLQLINES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V69</accession>
    <name>Q97V69_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thioredoxin reductase (TrxB-2)</fullName>
            <ecNumber evidence="4">1.8.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">trxB-2</name>
        <name evidence="4" type="ordered locus">SSO2765</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.1.9" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90452">
        <property type="entry name" value="E90452"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923960.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V69"/>
    <dbReference type="FunCoup" id="Q97V69">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2765"/>
    <dbReference type="PaxDb" id="273057-SSO2765"/>
    <dbReference type="EnsemblBacteria" id="AAK42876">
        <property type="protein sequence ID" value="AAK42876"/>
        <property type="gene ID" value="SSO2765"/>
    </dbReference>
    <dbReference type="GeneID" id="1452795"/>
    <dbReference type="KEGG" id="sso:SSO2765"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2846"/>
    <dbReference type="eggNOG" id="arCOG01296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_3_2"/>
    <dbReference type="InParanoid" id="Q97V69"/>
    <dbReference type="PhylomeDB" id="Q97V69"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="3">
        <location>
            <begin position="6"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="325" mass="35229" checksum="7DA8A0803D94F9B5" modified="2001-10-01" version="1">MPLKTYDTIIVGAGIAGLSAALYSSRQKLSTLVLSKDLGGQLTLTDLIENYPGIESTGGLTLAQKIEKQAKKFGAEFIYGEEVKEIAQESDLFIIKGIKGEYAGRALILAFGKTPREINVPGEQEFKGKGVSYCAICDAAFFKGKPAAVIGEGEPGIEAIELLSNYANPAYYITSSSYLAGEEEIVKNVVNKPTVKILTSSRVLEIRGNSKVEELVIKRGDEILQLKVDGVIIEMGYTLKTEFLKGFVELNEKGEIIVDELGRTSREGVFAAGDVTQTPYKQAVVAAAEGVKAALSAYNYIRSKRGLPPVTVDWKAEKKKVSFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V86</accession>
    <name>Q97V86_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Linocin_M18 bacteriocin protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2749</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Encapsulin nanocompartment</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90450">
        <property type="entry name" value="D90450"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990264.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V86"/>
    <dbReference type="STRING" id="273057.SSO2749"/>
    <dbReference type="PaxDb" id="273057-SSO2749"/>
    <dbReference type="EnsemblBacteria" id="AAK42859">
        <property type="protein sequence ID" value="AAK42859"/>
        <property type="gene ID" value="SSO2749"/>
    </dbReference>
    <dbReference type="GeneID" id="1452778"/>
    <dbReference type="KEGG" id="sso:SSO2749"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2828"/>
    <dbReference type="eggNOG" id="arCOG05908">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067492_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V86"/>
    <dbReference type="PhylomeDB" id="Q97V86"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140737">
        <property type="term" value="C:encapsulin nanocompartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.10">
        <property type="entry name" value="hypothetical protein PF0899 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007544">
        <property type="entry name" value="ENCAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051429">
        <property type="entry name" value="Encapsulin_nc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37165">
        <property type="entry name" value="PEPTIDASE U56 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37165:SF1">
        <property type="entry name" value="TYPE 1 ENCAPSULIN SHELL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04454">
        <property type="entry name" value="Linocin_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1284">Encapsulin nanocompartment</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00033738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="39491" checksum="FC84D2B10287124E" modified="2001-10-01" version="1">MFSNDPSTLTRKEKFDKEESVRAIRNAIAAEIDAINFYLQQNKYIDDEKLRKVHEDITKEEMTHFGEFLRLLYQVSPEDFSYFKKGWEEASKLIGETKDFPLIPTSSKEISKDHIVSWITEGIVSSRIMRNIGNTIKYEFTTIPLSEIKEDSGDIIQSKSASLYEVPLINSQVKFYLGQKSDSRRTAVLAGKSFAKMENYLLLKNHPLSPLKIGLKITGSDWNVAGNILLDVLRAYENLTREGFGKDVYILMSSLNYSKTFRVVDRSGTYEIEMIKEIGNVVPTDIVSNDEIYVISKQGFDILVFSDLNVEYLSKEKDYEVYLITEQIAPRLISSAASCVITQKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VD1</accession>
    <name>Q97VD1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Permease, major facilitator superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2701</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90444">
        <property type="entry name" value="F90444"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988626.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VD1"/>
    <dbReference type="FunCoup" id="Q97VD1">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2701"/>
    <dbReference type="PaxDb" id="273057-SSO2701"/>
    <dbReference type="EnsemblBacteria" id="AAK42813">
        <property type="protein sequence ID" value="AAK42813"/>
        <property type="gene ID" value="SSO2701"/>
    </dbReference>
    <dbReference type="GeneID" id="1452732"/>
    <dbReference type="KEGG" id="sso:SSO2701"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2781"/>
    <dbReference type="eggNOG" id="arCOG00132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025894_3_0_2"/>
    <dbReference type="InParanoid" id="Q97VD1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520:SF5">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="391" mass="41613" checksum="286B7F7DB5FBB4B9" modified="2001-10-01" version="1">MQDDYGYTFLLLSRILRSIGIIYITLSSSLYLSAIGVKPEIIGIIFLGAIGFSSTLNLALGIIGDRIGYKKVLIFVEILAAIASMLLALSINQTLIFAALIIGGVGGAAGGIRGVFSPGLTALVASNWSEEKERVRRLSFLMSASSLAGIGGSVLLSIRSYISNSVEGYRLLYFLSFALLLASAISLLLVKEVKRPKKTSKVMKKSSMKYISKVIVSNSITGFGLGIAIPLLPLWYKLAFHANSFEIGLIFTISYLTTAIGSLLSNKIMFDPLKVASITRILNGVFLIAMAFSPWLALAAGLYIARGLNAGIGAPNRSAVNVRGVSAEDFGTASSFQGLATRLSQMSSGISGYLLDISYSLPLEIGGLLQAVGGYLYMKLLAISTDRKVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VG0</accession>
    <name>Q97VG0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99441">
        <property type="entry name" value="A99441"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988583.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VG0"/>
    <dbReference type="STRING" id="273057.SSO2666"/>
    <dbReference type="PaxDb" id="273057-SSO2666"/>
    <dbReference type="EnsemblBacteria" id="AAK42784">
        <property type="protein sequence ID" value="AAK42784"/>
        <property type="gene ID" value="SSO2666"/>
    </dbReference>
    <dbReference type="GeneID" id="1452703"/>
    <dbReference type="KEGG" id="sso:SSO2666"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2748"/>
    <dbReference type="eggNOG" id="arCOG08525">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1674062_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VG0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="157" mass="17611" checksum="BA3A7FF56F156235" modified="2001-10-01" version="1">MRRRTILAISITILAIGLIDVLFTYLYSSLVVYSLRDNFSLAEEGAQSFPILVKQNDKIIIEGNSSKPIDIYIIGIVPQKVANNVSSFSISYVSPNTGELYIQFRSLPYSNLTTISFTIKVFNSWFSGIGYLSSAIILFVGLILLGYAVQLKERRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VJ5</accession>
    <name>Q97VJ5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DsrE family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2628</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90436">
        <property type="entry name" value="F90436"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989355.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VJ5"/>
    <dbReference type="STRING" id="273057.SSO2628"/>
    <dbReference type="PaxDb" id="273057-SSO2628"/>
    <dbReference type="EnsemblBacteria" id="AAK42749">
        <property type="protein sequence ID" value="AAK42749"/>
        <property type="gene ID" value="SSO2628"/>
    </dbReference>
    <dbReference type="GeneID" id="1452668"/>
    <dbReference type="KEGG" id="sso:SSO2628"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2711"/>
    <dbReference type="eggNOG" id="arCOG01670">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2079529_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VJ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="13454" checksum="28E525929436F7BB" modified="2001-10-01" version="1">MAKLLFVIMDDPNNLSESIRAAHALHYAVELKKEGHEVYVYFDGLGTKIPISESPYKGLRPAYERALKEEIILGACGYCASPPHLNIRDKLINSIKLIGDEEHHYAFIDLINGGFQIIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VK6</accession>
    <name>Q97VK6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dipeptide ABC transporter permease protein (DppB-3)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dppB-3</name>
        <name evidence="9" type="ordered locus">SSO2618</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90435">
        <property type="entry name" value="C90435"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989342.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VK6"/>
    <dbReference type="FunCoup" id="Q97VK6">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2618"/>
    <dbReference type="PaxDb" id="273057-SSO2618"/>
    <dbReference type="EnsemblBacteria" id="AAK42738">
        <property type="protein sequence ID" value="AAK42738"/>
        <property type="gene ID" value="SSO2618"/>
    </dbReference>
    <dbReference type="GeneID" id="1454065"/>
    <dbReference type="KEGG" id="sso:SSO2618"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2699"/>
    <dbReference type="eggNOG" id="arCOG00751">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_0_3_2"/>
    <dbReference type="InParanoid" id="Q97VK6"/>
    <dbReference type="PhylomeDB" id="Q97VK6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163:SF6">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="254"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="102"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="343" mass="37916" checksum="CCC4A2A194CB79ED" modified="2001-10-01" version="1">MSINIVFFIIRRIINAFITLILLIILVFIMIHIIAPNPLALARLYTGPHATYTELEQVAKQYGLDQPLYIQIINYIINVLHGNFGIDPTYKVPVITLIGKYLPRTLELVIPATILSVVIGLFTGAIAASNRNKPLDYLVRGVYLVTWASPPFLIAVILQLVIAYYLRLLPPTGTVNPVLTPPKSITPFPLLNAMLAGDWPYFSSLVHHMVLPVIAIALVSFGIVTRIARASMLDYMESDFAKLSLMKGLSRRRVVYGVVLRNASIPLVTLIALLFGYSVAGAVVIEDIFQYHGMGYFITQAIESLDYTSILGTTIIVGIAIIIANLIADILYGVLDPRVRIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VU3</accession>
    <name>Q97VU3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sugar transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2516</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90423">
        <property type="entry name" value="H90423"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988487.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VU3"/>
    <dbReference type="FunCoup" id="Q97VU3">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2516"/>
    <dbReference type="PaxDb" id="273057-SSO2516"/>
    <dbReference type="EnsemblBacteria" id="AAK42647">
        <property type="protein sequence ID" value="AAK42647"/>
        <property type="gene ID" value="SSO2516"/>
    </dbReference>
    <dbReference type="GeneID" id="1453974"/>
    <dbReference type="KEGG" id="sso:SSO2516"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2593"/>
    <dbReference type="eggNOG" id="arCOG02687">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_2"/>
    <dbReference type="InParanoid" id="Q97VU3"/>
    <dbReference type="PhylomeDB" id="Q97VU3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF34">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="21"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="488" mass="53815" checksum="A49DBE7DC941B5FC" modified="2001-10-01" version="1">MYKARKLTFIRYFYELTGVSTSMSSKLDEYLARIDRLPTWGLSYAVLWALGIGYFATLYDAVSNLGYALPFIPFINATQASIIVSVGLAGYIAGSIGFGVASDRIGRRPILITTFALLGIGSLGMGLATNYFMLFIFRLLEGIGIGAALNLAMVYVAEFSPSMKRGKYANWIFISGWTAVGVGTVAAAFIVTINPTVGWRIIFLLPAVLSLMTTVVLTILSPESVRILVKKGKVQEVEKIVNKMEEISMRRARMESLPQPKIIHYEETPSSSQLKILRESKYLKRLISLTVFWFFIYFIQYTSTGLGPTFVKVVIGFSTAQYAEYIRLSGFAAIGATIVSFILLSVVEKTDRRLLTQIGGIGFLVSSFVSTYLLLNKELIVWFIAYFLLEFVVNPPYLAGYLMSSEAFPTAARSTAFAITDGIGHLGGVIGPLLLFPLIRIIGPLPAWVVLGLPVPFAAALLWFTIPKTVKVRLEEVNEAYREGTAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VV0</accession>
    <name>Q97VV0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fis family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2507</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99423">
        <property type="entry name" value="A99423"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989901.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VV0"/>
    <dbReference type="PaxDb" id="273057-SSO2507"/>
    <dbReference type="EnsemblBacteria" id="AAK42640">
        <property type="protein sequence ID" value="AAK42640"/>
        <property type="gene ID" value="SSO2507"/>
    </dbReference>
    <dbReference type="GeneID" id="1453967"/>
    <dbReference type="KEGG" id="sso:SSO2507"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2586"/>
    <dbReference type="eggNOG" id="arCOG01842">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128031_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VV0"/>
    <dbReference type="PhylomeDB" id="Q97VV0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="16390" checksum="9600DC81116E779D" modified="2001-10-01" version="1">MAEFLYPSREWAEEWCKLLNESKEYNDAGKGWVSPILFVVTDIPTQVLEHLGVKGTNTLAMKLYLNNGKCQGIEFFTDVTKADAPYVLEASYNSWKDIISGKLQVVSALLSGTIKLKKGSLFDLARYTTASVVMANVSNKINTKFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VV9</accession>
    <name>Q97VV9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">acetyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">acaB-5</name>
        <name evidence="8" type="ordered locus">SSO2496</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90421">
        <property type="entry name" value="H90421"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923899.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VV9"/>
    <dbReference type="FunCoup" id="Q97VV9">
        <property type="interactions" value="238"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2496"/>
    <dbReference type="PaxDb" id="273057-SSO2496"/>
    <dbReference type="EnsemblBacteria" id="AAK42631">
        <property type="protein sequence ID" value="AAK42631"/>
        <property type="gene ID" value="SSO2496"/>
    </dbReference>
    <dbReference type="GeneID" id="84057831"/>
    <dbReference type="KEGG" id="sso:SSO2496"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2576"/>
    <dbReference type="eggNOG" id="arCOG01282">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_1_1_2"/>
    <dbReference type="InParanoid" id="Q97VV9"/>
    <dbReference type="PhylomeDB" id="Q97VV9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000013">
        <property type="entry name" value="Acetyl-CoA acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050215">
        <property type="entry name" value="Thiolase-like_sf_Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005033">
        <property type="entry name" value="PRK06445.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853:SF2">
        <property type="entry name" value="3-OXOADIPYL-COA_3-OXO-5,6-DEHYDROSUBERYL-COA THIOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="7">
        <location>
            <begin position="272"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="395" mass="43145" checksum="6DD8FF1BBDB076DA" modified="2001-10-01" version="1">MLEDVYLVDYARTAFTRFSRKDYQKDPFYNIRPEELAGMVINRLIEKNGIKAEEIDEIITGCALQVGEQWAFGGRHEIFAARLPYNIPTMAVDRQCASSLTTVSIGAMEISTGMADIVLAGGVEKLSRTPMFDNPHIEVNTKFLTDSKYIEYDLTTGYVMGLTAEKLAEEARIKREEMDRWSLRSHQLAWKAIQEGYFKDEILPIEVEVEGKKTVVNVDQSVRPDTSIEKLGQLPPAFKPNGTITAGNSAPLNSGASYVLLMSKNALKKYGLTPMAKIRSFGFAGVPPAVMGKGPVPASKKALEKANLSTRKIDLWEINEAFAVVVLYAIKQLELDESTVNKKGGAIAIGHPLGATGARLVGTLARQLIIEGKDYGVATLCVGGGQGGAIVLERV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W62</accession>
    <name>IF1A_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Translation initiation factor 1A</fullName>
            <shortName>aIF-1A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">eIF1A</name>
        <name type="ordered locus">SSO2375</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-1A family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90408">
        <property type="entry name" value="G90408"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989464.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W62"/>
    <dbReference type="SMR" id="Q97W62"/>
    <dbReference type="FunCoup" id="Q97W62">
        <property type="interactions" value="212"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2375"/>
    <dbReference type="PaxDb" id="273057-SSO2375"/>
    <dbReference type="EnsemblBacteria" id="AAK42526">
        <property type="protein sequence ID" value="AAK42526"/>
        <property type="gene ID" value="SSO2375"/>
    </dbReference>
    <dbReference type="GeneID" id="1453853"/>
    <dbReference type="KEGG" id="sso:SSO2375"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2460"/>
    <dbReference type="eggNOG" id="arCOG01179">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109098_1_2_2"/>
    <dbReference type="InParanoid" id="Q97W62"/>
    <dbReference type="PhylomeDB" id="Q97W62"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018104">
        <property type="entry name" value="TIF_eIF-1A_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003082">
        <property type="entry name" value="PRK04012.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003084">
        <property type="entry name" value="PRK04012.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003085">
        <property type="entry name" value="PRK04012.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01262">
        <property type="entry name" value="IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000145134" description="Translation initiation factor 1A">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="S1-like">
        <location>
            <begin position="11"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="108" mass="12518" checksum="EC6ADC11A358B3D8" modified="2001-10-01" version="1">MPKKDRAQEAPSRDVPRPEEGQTICVVKKMLGGDHLIVLCMDGKERLARIPGKIRKKMWMREGDVVLVGIWDFQPNRCDILYKYGNDEIKRLVNENIISREVIDQLRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-08-14" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W96</accession>
    <name>RF1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Peptide chain release factor subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Translation termination factor aRF1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">prf1</name>
        <name type="ordered locus">SSO2339</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of two subunits, one of which binds GTP.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-15910165">
            <id>Q97W96</id>
        </interactant>
        <interactant intactId="EBI-15910144">
            <id>Q980K5</id>
            <label>SSO0287</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>2</experiments>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="frameshift">
            <sequence resource="EMBL-CDS" id="AAK42492" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42492.1"/>
        <property type="status" value="ALT_FRAME"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90404">
        <property type="entry name" value="E90404"/>
    </dbReference>
    <dbReference type="DIP" id="DIP-60311N"/>
    <dbReference type="FunCoup" id="Q97W96">
        <property type="interactions" value="344"/>
    </dbReference>
    <dbReference type="IntAct" id="Q97W96">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2339"/>
    <dbReference type="PaxDb" id="273057-SSO2339"/>
    <dbReference type="EnsemblBacteria" id="AAK42492">
        <property type="protein sequence ID" value="AAK42492"/>
        <property type="gene ID" value="SSO2339"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2339"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2425"/>
    <dbReference type="eggNOG" id="arCOG01742">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035759_3_0_2"/>
    <dbReference type="InParanoid" id="Q97W96"/>
    <dbReference type="PhylomeDB" id="Q97W96"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018444">
        <property type="term" value="C:translation release factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000003">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="entry name" value="eRF1 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00424">
        <property type="entry name" value="Rel_fact_arch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020918">
        <property type="entry name" value="Peptide_chain-rel_aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03676">
        <property type="entry name" value="aRF1_eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000143185" description="Peptide chain release factor subunit 1">
        <location>
            <begin position="1"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="369" mass="42310" checksum="EFE1726D2E97000F" modified="2001-08-14" version="1">MSXDEYALNKQELKALIKELKKWKAPATVLLSLYIPPGRPIPDVVNLLRQEYSIAQNIKLKRTRDAVLSAIGAAIDRLNKIPKIDGNGLVLFCGENFDTEDFKCFMFSPPDKVPLFFYRTDKEFHLEFLEDMVEDTAVYGLIIVERDEATIGLLKGARIEILEEIEGFVPGKHMMGGQSQRRIDRIIDEMYHNFLKEVGEKVNAYFMPFVQNGKMKGVLLGGPGYAKEDFYKEDYVDYRIKNLILQPLIDVSDQGEVGLREMIMKAEDLLKNQQYIEVEKLLEELKYHLAKDDGLIIYGKEQIKKAMEIGAIEAIVIHEDSTDKELEKLAQDAENYGVKVFVVGDEVPEAEWVKKTFNGIVGKLRYRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WG2</accession>
    <name>Q97WG2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2259</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90396">
        <property type="entry name" value="B90396"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WG2"/>
    <dbReference type="STRING" id="273057.SSO2259"/>
    <dbReference type="PaxDb" id="273057-SSO2259"/>
    <dbReference type="EnsemblBacteria" id="AAK42425">
        <property type="protein sequence ID" value="AAK42425"/>
        <property type="gene ID" value="SSO2259"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2259"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2352"/>
    <dbReference type="eggNOG" id="arCOG02115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1253639_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WG2"/>
    <dbReference type="PhylomeDB" id="Q97WG2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012440">
        <property type="entry name" value="DUF1641"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38433">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38433:SF1">
        <property type="entry name" value="DUF1641 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07849">
        <property type="entry name" value="DUF1641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="24900" checksum="ECC55875E903DF2B" modified="2001-10-01" version="1">MTNGEFYALDNLLKDEKLNSLNKILDVINTTDRLGILDVIKGILEDENTIGKIIGSLTSDDVLELLVNWDKVIKTTKLFINEDNIYNIQFLINLIDKVRSKGILDPIIGLLEDEESLGKIINALINDFTLNLINHWEEIINDLSRIDLTNFKYYTLLVSATGEALKTENVKPITSIWEIYKLLKDPDIQRGLGVAASVLKRIGKLYVPDKGLAFEVEKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WG3</accession>
    <name>Q97WG3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2257</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99396">
        <property type="entry name" value="A99396"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991531.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WG3"/>
    <dbReference type="STRING" id="273057.SSO2257"/>
    <dbReference type="PaxDb" id="273057-SSO2257"/>
    <dbReference type="EnsemblBacteria" id="AAK42424">
        <property type="protein sequence ID" value="AAK42424"/>
        <property type="gene ID" value="SSO2257"/>
    </dbReference>
    <dbReference type="GeneID" id="1453751"/>
    <dbReference type="KEGG" id="sso:SSO2257"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2351"/>
    <dbReference type="eggNOG" id="arCOG04328">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084678_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WG3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="30913" checksum="490B9576E34230AD" modified="2001-10-01" version="1">MRILVNGLLPLDSGKTTFSLSIIRLFNQVGIRLFPFKPIAGHNAWYSFNTLIRSEELGILVGNDALKYYDETKYDIRRINPFAVLFVPIDLEKISYNVSLYNMIMEYGFPYLIRLSDCINGTDQYFVNSSADIYVPKSMLKFTRNLALKFNAKLYDKMREIIDASPQLVDNCTIDVFRNYENVIIESYNDSASPTYNSTNVDYVFTVSPTKAFLIKGQEFKKALSLFSIPPWNVRVSTLMKYLKIGKSFEIDVGERVAKDEILDILLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WK2</accession>
    <name>Q97WK2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2214</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90391">
        <property type="entry name" value="A90391"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WK2"/>
    <dbReference type="PaxDb" id="273057-SSO2214"/>
    <dbReference type="EnsemblBacteria" id="AAK42384">
        <property type="protein sequence ID" value="AAK42384"/>
        <property type="gene ID" value="SSO2214"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2214"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2310"/>
    <dbReference type="eggNOG" id="arCOG05884">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165243_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WK2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="121" mass="14011" checksum="8AEEA7459359690B" modified="2001-10-01" version="1">MSFWEYYRMVEKGTLVSIEEFKSNRKLKESVKNGIKGLVKLLFQEADKIIKFDSNEDLIFQLMKLGLISPTLAQELLDILKIADNLDNVDDEILYSMLVRIMEDVEEAINNIGKYMVKNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WL9</accession>
    <name>Q97WL9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Erythrocyte band 7 membrane protein homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2195</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90388">
        <property type="entry name" value="H90388"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992097.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WL9"/>
    <dbReference type="FunCoup" id="Q97WL9">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2195"/>
    <dbReference type="PaxDb" id="273057-SSO2195"/>
    <dbReference type="EnsemblBacteria" id="AAK42367">
        <property type="protein sequence ID" value="AAK42367"/>
        <property type="gene ID" value="SSO2195"/>
    </dbReference>
    <dbReference type="GeneID" id="1453694"/>
    <dbReference type="KEGG" id="sso:SSO2195"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2291"/>
    <dbReference type="eggNOG" id="arCOG01915">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024949_3_3_2"/>
    <dbReference type="InParanoid" id="Q97WL9"/>
    <dbReference type="PhylomeDB" id="Q97WL9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08826">
        <property type="entry name" value="SPFH_eoslipins_u1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF19">
        <property type="entry name" value="AT06885P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="4">
        <location>
            <begin position="24"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="267" mass="29664" checksum="F485C1E07E92674A" modified="2001-10-01" version="1">MAISIGDIVGIVFLIIIIIIFIAMSFRVVREWERAVVLRLGRFLRVKGPGIIFLIPFVDRPLVVDLRVNTVEVPPQTILTKDNVTVSVDAVVYYKVVDPQKAVLSVFNYNVAVLNLAQTSLRDIVGQMELDELLSKREEINKRIQEILDVTTEGWGIKVTAVTIRDIRLSQDLLSAMAKQAEAERLRRAKVILSEGERQAASILADASAYYKNNPSALQLRFLETLSDISQRGGLIIVVPAGNEIYPTLGTSAALSTLSKKFQTETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WP5</accession>
    <name>Q97WP5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transmembrane type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2135</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90382">
        <property type="entry name" value="C90382"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988330.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WP5"/>
    <dbReference type="STRING" id="273057.SSO2135"/>
    <dbReference type="PaxDb" id="273057-SSO2135"/>
    <dbReference type="DNASU" id="1453641"/>
    <dbReference type="EnsemblBacteria" id="AAK42314">
        <property type="protein sequence ID" value="AAK42314"/>
        <property type="gene ID" value="SSO2135"/>
    </dbReference>
    <dbReference type="GeneID" id="1453641"/>
    <dbReference type="KEGG" id="sso:SSO2135"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2227"/>
    <dbReference type="eggNOG" id="arCOG01467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_2_3_2"/>
    <dbReference type="InParanoid" id="Q97WP5"/>
    <dbReference type="PhylomeDB" id="Q97WP5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051328">
        <property type="entry name" value="T7SS_ABC-Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43077:SF10">
        <property type="entry name" value="TRANSPORT PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43077">
        <property type="entry name" value="TRANSPORT PERMEASE YVFS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="6">
        <location>
            <begin position="31"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="290" mass="32327" checksum="7A26C82F6E155BBD" modified="2001-10-01" version="1">MIEEERNNYSVFHGLWTLASRELKKWYKAPVILLLSLIQPIFWIGIFGKAMNLGSIFTETTFNIPGLTIPKQVLDQIGLEILKQTFGTTDYFSFLAAGMLSFIVLFTSMQSGMSIVWDRRLGVLDRILTTPVPRGNIIIGKVLSAVIRSLVQATIVLVVAVLLGMTFAPEINPLDFLGVYAALFLMSFGLSSLFLMLALRATSWESQMAIMNLLNLPLLFTSNAFYPVKSMPYWLKPVAYINPLTYSNGVARGMLLGISTNLAIDFLYLGLFAIILSTIGIVLSWKYLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WP7</accession>
    <name>Q97WP7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallophosphoesterase TT1561-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99376">
        <property type="entry name" value="A99376"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WP7"/>
    <dbReference type="STRING" id="273057.SSO2085"/>
    <dbReference type="PaxDb" id="273057-SSO2085"/>
    <dbReference type="EnsemblBacteria" id="AAK42264">
        <property type="protein sequence ID" value="AAK42264"/>
        <property type="gene ID" value="SSO2085"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2085"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2161"/>
    <dbReference type="eggNOG" id="arCOG01145">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041441_5_0_2"/>
    <dbReference type="InParanoid" id="Q97WP7"/>
    <dbReference type="PhylomeDB" id="Q97WP7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029461">
        <property type="entry name" value="TT1561-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523:SF1">
        <property type="entry name" value="CALCINEURIN-LIKE PHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14582">
        <property type="entry name" value="Metallophos_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophosphoesterase TT1561-like" evidence="1">
        <location>
            <begin position="196"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="316" mass="35688" checksum="3734BABCA2BD03EE" modified="2001-10-01" version="1">MVKHHCRMVFKFKLGFFTDLHGSEYSFKRSLNIAKSKRVDFLIISGGLIAKEILFVEDIGGRYFLNGKKVNLNNLNEDALRSGKYIVVDKKEVIEDIKSDKSKLEKIVIEKASEQMSRWVKIAEEIFRLEKVFWSPAHGDIPQLDSILKNYGSNLINENVINLEEIQIVSLGFGSPIGNSYREIPDSELYIKGKSLLKDADKERLIMNFHMPPLNTKLDNAKVGLRKSHVGSKAVLDLITEFQPIISLHGHVHESTSMDKVGETIAINPGSLYQLGDPSIVTFEVHKELKSFGSVNVSKYVIKNIEFVSTNPLDVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="146" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WQ2</accession>
    <name>Q97WQ2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Indolepyruvate oxidoreductase subunit IorA</fullName>
            <shortName evidence="16">IOR</shortName>
            <ecNumber evidence="16">1.2.7.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 16">Indolepyruvate ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">iorA</name>
        <name evidence="19" type="ordered locus">SSO2067</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="function">
        <text evidence="1 16">Catalyzes the ferredoxin-dependent oxidative decarboxylation of arylpyruvates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 16">
            <text>indole-3-pyruvate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = (indol-3-yl)acetyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12645"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57271"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="EC" id="1.2.7.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="16 17">Binds 2 [4Fe-4S] clusters. In this family the first cluster has a non-standard and varying [4Fe-4S] binding motif CX(2)CX(2)CX(4-5)CP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 16">Heterodimer of the IorA and IorB subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.8" evidence="16"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90374">
        <property type="entry name" value="C90374"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990194.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WQ2"/>
    <dbReference type="STRING" id="273057.SSO2067"/>
    <dbReference type="PaxDb" id="273057-SSO2067"/>
    <dbReference type="EnsemblBacteria" id="AAK42250">
        <property type="protein sequence ID" value="AAK42250"/>
        <property type="gene ID" value="SSO2067"/>
    </dbReference>
    <dbReference type="GeneID" id="1453577"/>
    <dbReference type="GeneID" id="44130775"/>
    <dbReference type="KEGG" id="sso:SSO2067"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2145"/>
    <dbReference type="eggNOG" id="arCOG01609">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017727_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WQ2"/>
    <dbReference type="PhylomeDB" id="Q97WQ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043805">
        <property type="term" value="F:indolepyruvate ferredoxin oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02008">
        <property type="entry name" value="TPP_IOR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000077">
        <property type="entry name" value="Indolepyruvate oxidoreductase subunit IorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000039">
        <property type="entry name" value="Indolepyruvate oxidoreductase subunit IorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000109">
        <property type="entry name" value="Indolepyruvate oxidoreductase subunit IorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045025">
        <property type="entry name" value="HACL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017721">
        <property type="entry name" value="IorA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03336">
        <property type="entry name" value="IOR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43710">
        <property type="entry name" value="2-HYDROXYACYL-COA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43710:SF7">
        <property type="entry name" value="INDOLEPYRUVATE OXIDOREDUCTASE SUBUNIT IORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006439">
        <property type="entry name" value="Indolepyruvate_ferr_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 16 17" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9 16" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11 16 17" id="KW-0408">Iron</keyword>
    <keyword evidence="12 16 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 16 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="18">
        <location>
            <begin position="542"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="18">
        <location>
            <begin position="572"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="551"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="554"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="557"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="563"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="581"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="584"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="587"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="591"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR006439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR006439-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AAK42250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="612" mass="67123" checksum="A298D2B5EA635E32" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WV8</accession>
    <name>CAS6B_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated endoribonuclease Cas6 2</fullName>
            <ecNumber>3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cas6b</name>
        <name type="ordered locus">SSO2004</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="J. Biol. Chem." volume="286" first="21643" last="21656">
            <title>Structural and functional characterization of an archaeal clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for antiviral defense (CASCADE).</title>
            <authorList>
                <person name="Lintner N.G."/>
                <person name="Kerou M."/>
                <person name="Brumfield S.K."/>
                <person name="Graham S."/>
                <person name="Liu H."/>
                <person name="Naismith J.H."/>
                <person name="Sdano M."/>
                <person name="Peng N."/>
                <person name="She Q."/>
                <person name="Copie V."/>
                <person name="Young M.J."/>
                <person name="White M.F."/>
                <person name="Lawrence C.M."/>
            </authorList>
            <dbReference type="PubMed" id="21507944"/>
            <dbReference type="DOI" id="10.1074/jbc.M111.238485"/>
        </citation>
        <scope>FUNCTION AS AN ENDORIBONUCLEASE</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease, generating an 8 base-long tag known as the 5' handle.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text>Possibly part of the aCascade ribonucleoprotein complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated endoribonuclease Cas6 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
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        <property type="protein sequence ID" value="AAK42192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99367">
        <property type="entry name" value="A99367"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992200.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="4ILL">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.48 A"/>
        <property type="chains" value="A/B=1-289"/>
    </dbReference>
    <dbReference type="PDB" id="4ILM">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.07 A"/>
        <property type="chains" value="A/B/D/F/H/J/L/N=1-289"/>
    </dbReference>
    <dbReference type="PDB" id="4ILR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.09 A"/>
        <property type="chains" value="A=1-289"/>
    </dbReference>
    <dbReference type="PDBsum" id="4ILL"/>
    <dbReference type="PDBsum" id="4ILM"/>
    <dbReference type="PDBsum" id="4ILR"/>
    <dbReference type="AlphaFoldDB" id="Q97WV8"/>
    <dbReference type="SMR" id="Q97WV8"/>
    <dbReference type="DIP" id="DIP-60141N"/>
    <dbReference type="STRING" id="273057.SSO2004"/>
    <dbReference type="PaxDb" id="273057-SSO2004"/>
    <dbReference type="EnsemblBacteria" id="AAK42192">
        <property type="protein sequence ID" value="AAK42192"/>
        <property type="gene ID" value="SSO2004"/>
    </dbReference>
    <dbReference type="GeneID" id="1453519"/>
    <dbReference type="KEGG" id="sso:SSO2004"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2079"/>
    <dbReference type="eggNOG" id="arCOG01439">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_929391_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WV8"/>
    <dbReference type="EvolutionaryTrace" id="Q97WV8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09652">
        <property type="entry name" value="Cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041165">
        <property type="entry name" value="Cas6_N_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019267">
        <property type="entry name" value="CRISPR-assoc_Cas6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010156">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01877">
        <property type="entry name" value="cas_cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17952">
        <property type="entry name" value="Cas6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10040">
        <property type="entry name" value="CRISPR_Cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000417884" description="CRISPR-associated endoribonuclease Cas6 2">
        <location>
            <begin position="1"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="3"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="25"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="38"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="41"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="52"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="81"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="90"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="101"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="122"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="130"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="155"/>
            <end position="157"/>
        </location>
    </feature>
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        <location>
            <begin position="158"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="174"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="195"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="210"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="227"/>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="232"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="248"/>
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        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="273"/>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="277"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="21507944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="4ILL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="4ILM"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="4ILR"/>
        </source>
    </evidence>
    <sequence length="289" mass="32637" checksum="03ADDA6E415DDC83" modified="2001-10-01" version="1">MPLIFKIGYNVIPLQDVILPTPSSKVLKYLIQSGKLIPSLKDLITSRDKYKPIFISHLGFNQRRIFQTNGNLKTITKGSRLSSIIAFSTQANVLSEVADEGIFETVYGKFHIMIESIEIVEVEKLKEEVEKHMNDNIRVRFVSPTLLSSKVLLPPSLSERYKKIHAGYSTLPSVGLIVAYAYNVYCNLIGKKEVEVRAFKFGILSNALSRIIGYDLHPVTVAIGEDSKGNLRKARGVMGWIEFDIPDERLKRRALNYLLTSSYLGIGRSRGIGFGEIRLEFRKIEEKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WV9</accession>
    <name>Q97WV9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="RefSeq" id="WP_009992199.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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        <property type="gene ID" value="SSO2002"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="CRISPR-assoc_prot_CsaX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03876">
        <property type="entry name" value="cas_csaX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="345" mass="38776" checksum="945264E84E0F7C47" modified="2001-10-01" version="1">MEVPLYNIFGDNYIIQVATEAENSSIYNNKVEIDDDELRNVLNLAYKIAKNNEDAAAERRGKAKKKKGEEGETTTSNVILPLSGNDKNPWTETLKCYNFPTTVALSEVFKNFSQVKECEEVSAPSFVKPEFYEFGRSPGMVERTRRVKLEVEPHYLIIAAAGWVLTRLGKAMVSEGDYVGVNVFTPTRGILYSLIQNVNGIIPGIKPETAFGLWIARKVVSSVTNPNVSVMRIYTISDAAGQNPTTINGGFSIDLTKLLEKRDLLSERLEAIARNALSISSNMRERYIVLANYIYEYLTGSKRLEDLLYFANRDLIMNLNSDEERVRDLKLISTYVNGELIRGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WW3</accession>
    <name>Q97WW3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Type I-A CRISPR-associated protein Cas7/Csa2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1997</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="D90366"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991625.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WW3"/>
    <dbReference type="STRING" id="273057.SSO1997"/>
    <dbReference type="PaxDb" id="273057-SSO1997"/>
    <dbReference type="EnsemblBacteria" id="AAK42187">
        <property type="protein sequence ID" value="AAK42187"/>
        <property type="gene ID" value="SSO1997"/>
    </dbReference>
    <dbReference type="GeneID" id="1453514"/>
    <dbReference type="KEGG" id="sso:SSO1997"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2074"/>
    <dbReference type="eggNOG" id="arCOG03617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054331_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WW3"/>
    <dbReference type="PhylomeDB" id="Q97WW3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09685">
        <property type="entry name" value="Cas7_I-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002764">
        <property type="entry name" value="Cas7/Cst2/DevR_sub_I-a/Apern"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010154">
        <property type="entry name" value="CRISPR-assoc_Cas7/Cst2/DevR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052681">
        <property type="entry name" value="CRISPR-Cas7/Cst2/DevR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01875">
        <property type="entry name" value="cas_MJ0381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02583">
        <property type="entry name" value="DevR_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37459">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37459:SF1">
        <property type="entry name" value="CRISPR-ASSOCIATED PROTEIN CAS7_CST2_DEVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01905">
        <property type="entry name" value="DevR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="35210" checksum="B54F9A1C77A5FFA7" modified="2001-10-01" version="1">MIGGSGRFLVNLESLNGVESIGNLTKHRTAPVVLKTSTGYLVRYVPVISGEALAHAYQASLVDIAKKEGLPVGNLSSQYEFIKFSTDEALKIEGIKEPKDYNDARRFEVEVMLKDVIADVGGFMYAGSAPVRRTSRIKLGYMIPALRGDEIPAQLEAQFHVRFSNKPVKGSQAIFNVEVSSALYTFSFELDEDLIAVPSTVGEKVKGEEELERQKARRVDSAIKALYSLLAGNFGGKRSRFLPSMKLMSLVVVKTDFPFIPEPGHDDSYIKTTVMRLEKAKSVLNGNVAKVYVINNEGIEVGEGATVLSSVEDLVAKLKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X52</accession>
    <name>Q97X52_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetyl-CoA synthetase (Acetate-CoA ligase) amino-end. (AcsA-3)</fullName>
            <ecNumber evidence="6">6.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">acsA-3</name>
        <name evidence="6" type="ordered locus">SSO1903</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90354">
        <property type="entry name" value="G90354"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923641.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X52"/>
    <dbReference type="FunCoup" id="Q97X52">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1903"/>
    <dbReference type="PaxDb" id="273057-SSO1903"/>
    <dbReference type="EnsemblBacteria" id="AAK42094">
        <property type="protein sequence ID" value="AAK42094"/>
        <property type="gene ID" value="SSO1903"/>
    </dbReference>
    <dbReference type="GeneID" id="1453421"/>
    <dbReference type="KEGG" id="sso:SSO1903"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1962"/>
    <dbReference type="eggNOG" id="arCOG04201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_10_2"/>
    <dbReference type="InParanoid" id="Q97X52"/>
    <dbReference type="PhylomeDB" id="Q97X52"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015645">
        <property type="term" value="F:fatty acid ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000097">
        <property type="entry name" value="AMP-dependent synthetase and ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051087">
        <property type="entry name" value="Mitochondrial_ACSM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605:SF10">
        <property type="entry name" value="ACYL-COA SYNTHETASE MEDIUM CHAIN FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605">
        <property type="entry name" value="ACYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="43"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="457" mass="52244" checksum="3C708AF28F86FA4E" modified="2001-10-01" version="1">MVSYEEVRRSFSWNEVIKFLDKNPLDVLTSHDGIAIKRFTKDVVEDISFSDLKRKALRLALYLREFHDVKKGDVIAVLASKKIEQVIVLLATFSLGAIYQPLFTAFGPEAIKMRTRDAKPKVIFYQDDQKDKVSDAIPFSKFDELLSYGELKNIEKINWDDPIVLLYTSGTTGAPKGALIAKRLLLNTYVYMKYGIGIRDSDVFWNPADPGWAYGLYYGIIGPLMFGKTVIFLDEPFNPERTMEFMEEKKVTNFAFAPTAYRMIAGTIKRKYDLVLERASSAGEPLNPEVIRWFMDNYNVTIKDHYGQTEVGMVVYNGWGYETKIKIGSMGLPAPGYEIDVIEDTIAVKRDSPGFHFLGYLNNPEKTKESFRGDWYLTGDNAYKDEEGYFWFVGRKDDVVKVSGYRVGPFEVESVLLEFPAVLESAVVADEDPIRGQWCFTKLFHLKDIILVLSKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X56</accession>
    <name>Q97X56_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1286 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1899</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90354">
        <property type="entry name" value="B90354"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992018.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X56"/>
    <dbReference type="PaxDb" id="273057-SSO1899"/>
    <dbReference type="DNASU" id="1453416"/>
    <dbReference type="EnsemblBacteria" id="AAK42089">
        <property type="protein sequence ID" value="AAK42089"/>
        <property type="gene ID" value="SSO1899"/>
    </dbReference>
    <dbReference type="GeneID" id="1453416"/>
    <dbReference type="KEGG" id="sso:SSO1899"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1954"/>
    <dbReference type="eggNOG" id="arCOG05320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117041_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X56"/>
    <dbReference type="PhylomeDB" id="Q97X56"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009705">
        <property type="entry name" value="DUF1286"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06939">
        <property type="entry name" value="DUF1286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="20204" checksum="E0EE139D3169F94C" modified="2001-10-01" version="1">MRLRTHYVFSTGLLTLLDSGIFHEYFYYTLILCGIVSVIGNSLIDRIGHKEIITRYGYISMRTPLTHTIPRSVVWGIISIIPIFILLLIYYYGLNYHEYYLFSINNRVILLTLLNGIVVGPSHMLLDVFTERGIYVKKYGKWRRFALAHFKYDNPAINGLAIIVGIIMIYLAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X84</accession>
    <name>Q97X84_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90350">
        <property type="entry name" value="C90350"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X84"/>
    <dbReference type="STRING" id="273057.SSO1870"/>
    <dbReference type="PaxDb" id="273057-SSO1870"/>
    <dbReference type="EnsemblBacteria" id="AAK42058">
        <property type="protein sequence ID" value="AAK42058"/>
        <property type="gene ID" value="SSO1870"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1870"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1921"/>
    <dbReference type="eggNOG" id="arCOG03700">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081192_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X84"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="226" mass="25179" checksum="6432BBB2FA174543" modified="2001-10-01" version="1">MESKKLLLIGVIIAVVVASVVGYAYYYSYYLPVQSSVTYYNQQLQYMGYYAQGNFGMMGGGMMGGGGAYHMMGYYGGYATLYENTITISEAISMMRNVPSYVKVFPNNNTLLFTSSNIVLVVLGMGHERATNLTGQQPPAYAQHDVFVIYGLINPTLIIPRGATVQVVFINLDEDMLHNIAITPVPPPYPYYPMMYIRMNTVGMTPMLPYVNYNSGQAYEFSFTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XF2</accession>
    <name>Q97XF2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRISPR system endoribonuclease Csx1-like HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90340">
        <property type="entry name" value="G90340"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992507.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XF2"/>
    <dbReference type="STRING" id="273057.SSO1788"/>
    <dbReference type="PaxDb" id="273057-SSO1788"/>
    <dbReference type="EnsemblBacteria" id="AAK41982">
        <property type="protein sequence ID" value="AAK41982"/>
        <property type="gene ID" value="SSO1788"/>
    </dbReference>
    <dbReference type="GeneID" id="1454761"/>
    <dbReference type="KEGG" id="sso:SSO1788"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1843"/>
    <dbReference type="eggNOG" id="arCOG03433">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1782573_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XF2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3740.10">
        <property type="entry name" value="SSO1389-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027419">
        <property type="entry name" value="CRISPR-assoc_Csx1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019016">
        <property type="entry name" value="Csx1-like_HEPN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09455">
        <property type="entry name" value="Csx1_HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160980">
        <property type="entry name" value="SSO1389-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR system endoribonuclease Csx1-like HEPN" evidence="1">
        <location>
            <begin position="78"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="168" mass="19983" checksum="35C2BD6982100722" modified="2001-10-01" version="1">MVIEKCKEKKEFNNDYYQYFLNNVKIEGKEIKINEKLYPDRAWVDVICDYVCSKVKDNTKEEIEEFGKILEKFSMNAGPLISREINIIYNLAKDLKEGERKQYSEIYSEDEEGRSKQLSEEELKRNFIAHAGLLKEYVIIEKKNGKIYVNYDESKIKELLEWSVLKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XH0</accession>
    <name>Q97XH0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Second ORF in transposon ISC1491</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1769</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90338">
        <property type="entry name" value="E90338"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XH0"/>
    <dbReference type="PaxDb" id="273057-SSO1769"/>
    <dbReference type="EnsemblBacteria" id="AAK41964">
        <property type="protein sequence ID" value="AAK41964"/>
        <property type="gene ID" value="SSO1769"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1769"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1820"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153635_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XH0"/>
    <dbReference type="PhylomeDB" id="Q97XH0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF17">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="99" mass="11133" checksum="F09E05AF655915E1" modified="2001-10-01" version="1">MISAATIYAEFGDVSRFSSSKARAYAGFAPRTKQSGGSESHSGMIRGNKYLRRAFFLVARVARKSERFKEYYDRLVSRWKSATKLVLLVGSLLVFVITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XH2</accession>
    <name>Q97XH2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1767</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90338">
        <property type="entry name" value="C90338"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XH2"/>
    <dbReference type="PaxDb" id="273057-SSO1767"/>
    <dbReference type="EnsemblBacteria" id="AAK41962">
        <property type="protein sequence ID" value="AAK41962"/>
        <property type="gene ID" value="SSO1767"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1767"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1818"/>
    <dbReference type="eggNOG" id="arCOG07190">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_935740_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XH2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="300" mass="34975" checksum="C9089FA69D856FEB" modified="2001-10-01" version="1">MKMWVELDLNPILDKDLDLKRQVKEEIQKEKIDSTITIDLIRSLNKDILDVNALGLEDRDYNLYIWSLIDSYFVTGNNKSYELVNELLSKRKTLHSSLFQLKVYDITKDKSILTSVSDTIFKLDEYWGEDLLALAKLSYITQDLKIVKRSTEIMLNKLEEIERQGGIKSEIDVEMGMGALKGLSLININYSKYPDILEKIKYYDDKYFVPMFEFIGNKPNIPEYLDSLQVIPMLASSKEFTVFAATKDIKYLKGTIKLYKYYQEYLNTIGITKTSLRQKLWGLIALSRIVYFIEKGKILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XH8</accession>
    <name>Q97XH8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nitric oxide response protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90337">
        <property type="entry name" value="E90337"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989072.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XH8"/>
    <dbReference type="STRING" id="273057.SSO1763"/>
    <dbReference type="PaxDb" id="273057-SSO1763"/>
    <dbReference type="EnsemblBacteria" id="AAK41956">
        <property type="protein sequence ID" value="AAK41956"/>
        <property type="gene ID" value="SSO1763"/>
    </dbReference>
    <dbReference type="GeneID" id="1454735"/>
    <dbReference type="KEGG" id="sso:SSO1763"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1810"/>
    <dbReference type="eggNOG" id="arCOG05707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_777599_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XH8"/>
    <dbReference type="PhylomeDB" id="Q97XH8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="38960" checksum="F55086FC28AA7BD0" modified="2001-10-01" version="1">MRLPLLLMIVGILLLLLGGIPAVFEFMDMQGFFLDPTASLFPAHWFIMIYGFFGALIGNEILVALSVEWSGKTADNKLIVIYLLSIILASISFLFISQQLGFIFTLLGMAILLYYSREYLGTSRLGLQPTTYNWLLFYSIMISTAIVALQLGLGYAIPYVNLIFPASMILAVMDRDVALVTGIKIARHWENVLAFILLVIGMGVYPNGSILMIIAWILSFHASGLYRFKGRKYPILHLTTAWIYLLLASIFIFNYDVYIHALAVGFLFNTVFGVDVVLMDLFVNAFERRVRIKPSYVPFVLVNLGLIMRFLYDFGLSIPILLLSAPLQGIGILSFFALTLKQVVMAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XI7</accession>
    <name>Q97XI7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1078</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90336">
        <property type="entry name" value="B90336"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XI7"/>
    <dbReference type="SMR" id="Q97XI7"/>
    <dbReference type="STRING" id="273057.SSO1749"/>
    <dbReference type="PaxDb" id="273057-SSO1749"/>
    <dbReference type="EnsemblBacteria" id="AAK41945">
        <property type="protein sequence ID" value="AAK41945"/>
        <property type="gene ID" value="SSO1749"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1749"/>
    <dbReference type="eggNOG" id="arCOG02129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431705_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XI7"/>
    <dbReference type="PhylomeDB" id="Q97XI7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="22"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="189" mass="21705" checksum="78F4E08C73D10282" modified="2001-10-01" version="1">MGEGTRGEAFKKIREGYKVHIFDESYVSYINKGKGWMKVGGGIKVNPKRKRFAVVGGITISKEGITFSYSTYKKPSLNSKDIILYLRKVIKDEKTVIVMDNARIHGNEVREFLEENGVEFVYLPPYSPDKSPAEGPWAVLKKRLYSKIYTDFETLMWDATRFLRSISKRVNDLTYSVKRCGEPLDVNTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XP8</accession>
    <name>Q97XP8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-methylhydantoinase A (HuyA-1)</fullName>
            <ecNumber evidence="4">3.5.2.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">huyA-1</name>
        <name evidence="4" type="ordered locus">SSO1663</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.14" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90327">
        <property type="entry name" value="D90327"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990098.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XP8"/>
    <dbReference type="FunCoup" id="Q97XP8">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1663"/>
    <dbReference type="PaxDb" id="273057-SSO1663"/>
    <dbReference type="EnsemblBacteria" id="AAK41875">
        <property type="protein sequence ID" value="AAK41875"/>
        <property type="gene ID" value="SSO1663"/>
    </dbReference>
    <dbReference type="GeneID" id="1454654"/>
    <dbReference type="KEGG" id="sso:SSO1663"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1712"/>
    <dbReference type="eggNOG" id="arCOG01511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002157_1_2_2"/>
    <dbReference type="InParanoid" id="Q97XP8"/>
    <dbReference type="PhylomeDB" id="Q97XP8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047423">
        <property type="term" value="F:N-methylhydantoinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049517">
        <property type="entry name" value="ACX-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF2">
        <property type="entry name" value="5-OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19278">
        <property type="entry name" value="Hydant_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="1">
        <location>
            <begin position="198"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Acetophenone carboxylase-like C-terminal" evidence="3">
        <location>
            <begin position="502"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="683" mass="76406" checksum="A6F71D2E0287738E" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XS9</accession>
    <name>Q97XS9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glyceraldehyde-3-phosphate dehydrogenase, NADP dependent (GapN-1)</fullName>
            <ecNumber evidence="6">1.2.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">gapN-1</name>
        <name evidence="6" type="ordered locus">SSO1629</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.9" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90323">
        <property type="entry name" value="D90323"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XS9"/>
    <dbReference type="FunCoup" id="Q97XS9">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1629"/>
    <dbReference type="PaxDb" id="273057-SSO1629"/>
    <dbReference type="EnsemblBacteria" id="AAK41843">
        <property type="protein sequence ID" value="AAK41843"/>
        <property type="gene ID" value="SSO1629"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1629"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1672"/>
    <dbReference type="eggNOG" id="arCOG01252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_5_3_2"/>
    <dbReference type="InParanoid" id="Q97XS9"/>
    <dbReference type="PhylomeDB" id="Q97XS9"/>
    <dbReference type="BRENDA" id="1.2.1.24">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="1.2.1.9">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008886">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (non-phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008911">
        <property type="term" value="F:lactaldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07145">
        <property type="entry name" value="ALDH_LactADH_F420-Bios"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000018">
        <property type="entry name" value="Alpha-aminoadipic semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000007">
        <property type="entry name" value="NAD/NADP-dependent betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051020">
        <property type="entry name" value="ALDH-related_metabolic_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42991">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42991:SF1">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="470" mass="51530" checksum="54BEE97B2D2FFE78" modified="2001-10-01" version="1">MMSQEVIEVRSPSNLNVIGTVKRMHKDEVRGEIEEAYKGFEIISKMPLYKRTAILRKVSEILEREQERLAKLLAMEAGKPIRDSRVEVLRASRLFRHAAEEVGMVLEGKNYRVDAYEYPPGNENRIVVSTREPIGVVTAILPFNFPINSFAHKVAPALAVGNSVVVKPSINTPLAAIEMKKILVEAGLPDSAVRVVTGYSSEIGDEIITHPLVGLITLTGSTQTGLKIASKAVSLGKRIIMELGGSDPIIILEDANIERASSIAVRARFEYAGQNCNAGKRIIVREEIYDKFVKAFNDKARALKVGDPLDETTDVGPVINKESVENLNSVLADAKAKGGKVEILNKGPESGSFFPLTMVTNPSLDMLVLKSEVFGPIVPIVSVKSDEEAIRIANSTEYGLQSAIFTNDVNRALKLSRELKFGAVIINDSTRLRWDSLAFGGFKKSSIGREGVRETMLEMTENKLIAITLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XX9</accession>
    <name>Q97XX9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Molybdopterin oxidoreductase, iron-sulfur binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1579</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90316">
        <property type="entry name" value="G90316"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923480.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XX9"/>
    <dbReference type="STRING" id="273057.SSO1579"/>
    <dbReference type="PaxDb" id="273057-SSO1579"/>
    <dbReference type="EnsemblBacteria" id="AAK41790">
        <property type="protein sequence ID" value="AAK41790"/>
        <property type="gene ID" value="SSO1579"/>
    </dbReference>
    <dbReference type="GeneID" id="7806190"/>
    <dbReference type="KEGG" id="sso:SSO1579"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1614"/>
    <dbReference type="eggNOG" id="arCOG01500">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_1_1_2"/>
    <dbReference type="InParanoid" id="Q97XX9"/>
    <dbReference type="PhylomeDB" id="Q97XX9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10551">
        <property type="entry name" value="PsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050954">
        <property type="entry name" value="ET_IronSulfur_Cluster-Binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43177">
        <property type="entry name" value="PROTEIN NRFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43177:SF3">
        <property type="entry name" value="PROTEIN NRFC HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="68"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="100"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="228" mass="25298" checksum="C79E2E40F6138980" modified="2001-10-01" version="1">MSTQVSNPYIQFGNTQPCKHWGFVVKVDQCLGCDACVAACTIENQTPFWRGLFRTHVEDLELGEYPNTQRVFVPRLCMQCENPPCYYVCPTGATQIVAGGIVVVDEYKCMGCLYCVEACPYGARYFYTYEDIEKSKEYFGLNSIHVVPHVDKCTFCYGTAPEGTYTPACVRTCPGHARVFGCLDDPNSEVSILVNTGQAVVLNPELNVQPKVFYVFNRQLGRGGGGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XY3</accession>
    <name>Q97XY3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nitric oxide reductase, subunit I (Cytochrome B) (NorB-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">norB-2</name>
        <name evidence="3" type="ordered locus">SSO1573</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90316">
        <property type="entry name" value="C90316"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923477.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XY3"/>
    <dbReference type="STRING" id="273057.SSO1573"/>
    <dbReference type="PaxDb" id="273057-SSO1573"/>
    <dbReference type="EnsemblBacteria" id="AAK41786">
        <property type="protein sequence ID" value="AAK41786"/>
        <property type="gene ID" value="SSO1573"/>
    </dbReference>
    <dbReference type="GeneID" id="34442704"/>
    <dbReference type="KEGG" id="sso:SSO1573"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1610"/>
    <dbReference type="eggNOG" id="arCOG04703">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1399798_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XY3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054309">
        <property type="entry name" value="NorB_cytochrome_c-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22085">
        <property type="entry name" value="NorB_cytochrome_c-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Nitric oxide reductase subunit B cytochrome c-like" evidence="2">
        <location>
            <begin position="40"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="194" mass="21648" checksum="26F6A9C1300BCD21" modified="2001-10-01" version="1">MAKRIKGDVWSNLVLVATVLVYVVYIALAGYTLTHLPPIPSVVETENGTVLFTGGEVISGKVLMQKYGLFDYGSFWGFGGYYGTDFTALALKVINQTTDPPTIKVDGPAYSSITDSETSRWVVSNNYVKAYNTLYNELCNILYNNSSNYGLKPNLVSPNDLRNITAFILWGAVVFHQIISFERYNISTFKKRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XZ1</accession>
    <name>Q97XZ1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90315">
        <property type="entry name" value="E90315"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XZ1"/>
    <dbReference type="STRING" id="273057.SSO1567"/>
    <dbReference type="PaxDb" id="273057-SSO1567"/>
    <dbReference type="EnsemblBacteria" id="AAK41780">
        <property type="protein sequence ID" value="AAK41780"/>
        <property type="gene ID" value="SSO1567"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1567"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1602"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_2_4_2"/>
    <dbReference type="InParanoid" id="Q97XZ1"/>
    <dbReference type="PhylomeDB" id="Q97XZ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07721">
        <property type="entry name" value="yflN-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF17">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="28"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="233" mass="26133" checksum="570DC35D19F9F88D" modified="2001-10-01" version="1">MGDGLMVKVGKVRIMELLEPEFFGTVLNHNISVIENGPCGGLMMIDTGLPGYLDQIESYLKAWGYSLEDISDIVITHWHHDHAGNAMAIKRISDAKIYAHVDELGDLENPPKYSTIYSDELGVSLPVFKRTMERINKLHYEPVKVDFALKGGEDLGGFRVIHVPGHTKGHIALFDGKCLVVGDAVRNVRNKLSPPLRIFSWNYELAVNSFNYLISLPYTVLIPFHGDIVIRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y44</accession>
    <name>Q97Y44_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1504</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90308">
        <property type="entry name" value="H90308"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923437.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y44"/>
    <dbReference type="PaxDb" id="273057-SSO1504"/>
    <dbReference type="EnsemblBacteria" id="AAK41727">
        <property type="protein sequence ID" value="AAK41727"/>
        <property type="gene ID" value="SSO1504"/>
    </dbReference>
    <dbReference type="GeneID" id="1454506"/>
    <dbReference type="KEGG" id="sso:SSO1504"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1545"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y44"/>
    <dbReference type="PhylomeDB" id="Q97Y44"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="175"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="300"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="391" mass="46031" checksum="129C2D31D32E84EA" modified="2001-10-01" version="1">MPTLGFRFRAYTDEQTLRALKAQLKSACKIYNTLRWADIYFYQRDGKGLTRTELRQLALDLRKQDDEYKQLYSQVVQQVADRYYEAKKRFFEGLAHFPKEKKPHKHYSLVYPQYGWKILQVREIRKGSKKKLITLKLSNLGTFKVIVHRDFPLDKVKRVVVKLTRSERIYITFVVDHEFPKLPNTGKVVAIDVGVEKLIVTSDGEYFPNLRPYEKALWKVKHLHRELSRKKFLSHNWFKAKVKLARAYEYLKNLRTDLYMKLGKWFAEHYDVVVMEGIHVKQLIGKSLRSLRRRLSDVAFSELRDLIKYQLEKYGKKLILVNPAYTSKTCAKCGYVKEDLSLSDRVFVCPNCGWIADRDYNASLNILRGSGSERPLVWSSALYQYSGMAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y46</accession>
    <name>Q97Y46_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1501</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90308">
        <property type="entry name" value="F90308"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990164.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y46"/>
    <dbReference type="PaxDb" id="273057-SSO1501"/>
    <dbReference type="EnsemblBacteria" id="AAK41725">
        <property type="protein sequence ID" value="AAK41725"/>
        <property type="gene ID" value="SSO1501"/>
    </dbReference>
    <dbReference type="GeneID" id="1454504"/>
    <dbReference type="KEGG" id="sso:SSO1501"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1543"/>
    <dbReference type="eggNOG" id="arCOG03724">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1217637_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y46"/>
    <dbReference type="PhylomeDB" id="Q97Y46"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1230">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58113">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="227" mass="27511" checksum="248438091397CE49" modified="2001-10-01" version="1">MADVFIQMLSDAVTEYAKSLVEKSKQGKKLSEQDYLFLMMYLNSKRTDDLRQDMNKKFDDLKSYVDKRFDDTKAEIISVKEDIKGLRDYTERKFDESRAYTDKKFEETNKKVEDLRHYVDNKFEETRVYTDKKFEETNKKVEDLRNYVDTKFQELRTYTDKKFEESRAYTDVQFVKFKEYVDKQFEHVDTRFNDLRSDMDSKFNELKNDLNLIIKLLVGKEKEEEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y66</accession>
    <name>Q97Y66_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1478</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90305">
        <property type="entry name" value="H90305"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923418.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y66"/>
    <dbReference type="PaxDb" id="273057-SSO1478"/>
    <dbReference type="EnsemblBacteria" id="AAK41703">
        <property type="protein sequence ID" value="AAK41703"/>
        <property type="gene ID" value="SSO1478"/>
    </dbReference>
    <dbReference type="GeneID" id="1454482"/>
    <dbReference type="KEGG" id="sso:SSO1478"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1515"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154343_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y66"/>
    <dbReference type="PhylomeDB" id="Q97Y66"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="147" mass="16712" checksum="7F7E2EE46FD1E211" modified="2001-10-01" version="1">MGFNDVFLLMGYTTSKLKNFTAFRKYKGSWGRKHGKSYFGFKVCNLVERRTNFVRDFKVGLANLSDLAFSFDNVKLMADRAWIFREDVLVKGVGSAGLPVEGSGVEIREGKASSTTLRGVVMEVFFLNFYRDLEILSTRIRTKVLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y87</accession>
    <name>Q97Y87_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISC1359</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1448</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90302">
        <property type="entry name" value="H90302"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y87"/>
    <dbReference type="SMR" id="Q97Y87"/>
    <dbReference type="PaxDb" id="273057-SSO1448"/>
    <dbReference type="EnsemblBacteria" id="AAK41679">
        <property type="protein sequence ID" value="AAK41679"/>
        <property type="gene ID" value="SSO1448"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1448"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1477"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067987_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y87"/>
    <dbReference type="PhylomeDB" id="Q97Y87"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033541">
        <property type="entry name" value="transpos_ISH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="114"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="388" mass="45115" checksum="28D3435C3E0DCD67" modified="2001-10-01" version="1">MKYRQNREDENFCRKVCIYEGGLEHMKHENTTKPAKFNRDFARSALKIIYSVLTKILFPEELLSALLKASGSYLSRLGKDGRRALRKLNAVQVEDVRDALRKMGRMTLRGVRDRRVAVDFHAIPQYHADKSFLSRIKPTKGTSWGLVQAAIFLLGRTRSFLDVIPVTVKNVAEGFKAVMEVIVKELEEDKLRLVMVFADREFAVNEVIRFLLELGLDFVISAKAQMYKKYKGMLQDVDVSFSGVRYTGFLCVRHGSGAYLIILRKEDGKIIAFLVRREMDLYDAIVLAEMYRERWGIENAFRSLEEFRIKTRTCDVRKELVLVLLSYLLLNVWFLIRSWRKVKLWEFSVSLSNLLDREVRVEQERAFREVKTSFPQTPANPMHLLPAT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2024-07-24" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YC7</accession>
    <name>CSA2A_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated protein Cas7/Csa2 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>CRISPR-associated aCascade subunit Cas7/Csa2, subtype I-A/Apern 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cas7a</name>
        <name type="ordered locus">SSO1399</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="J. Biol. Chem." volume="286" first="21643" last="21656">
            <title>Structural and functional characterization of an archaeal clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for antiviral defense (CASCADE).</title>
            <authorList>
                <person name="Lintner N.G."/>
                <person name="Kerou M."/>
                <person name="Brumfield S.K."/>
                <person name="Graham S."/>
                <person name="Liu H."/>
                <person name="Naismith J.H."/>
                <person name="Sdano M."/>
                <person name="Peng N."/>
                <person name="She Q."/>
                <person name="Copie V."/>
                <person name="Young M.J."/>
                <person name="White M.F."/>
                <person name="Lawrence C.M."/>
            </authorList>
            <dbReference type="PubMed" id="21507944"/>
            <dbReference type="DOI" id="10.1074/jbc.M111.238485"/>
        </citation>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35091 / DSM 1616 / JCM 8930 / NBRC 15331 / P1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the aCascade ribonucleoprotein complex, minimally composed of Csa2 and Cas5a, which binds crRNA. Other possible components of aCascade in strain P1 are Cas6b (SSO1437) and Csa5 (SSO1443), while SSO1399, Cas5b (SSO1400) and SSO1401 have sometimes been seen weakly associated. Csa2 is probably the major RNA-binding subunit. The Csa2-Cas5a-crRNA complex also binds target DNA homologous to crRNA, probably forming an R-loop. Purified aCascade forms a filament about 6 nm in width.</text>
    </comment>
    <comment type="miscellaneous">
        <text>The aCascade complex was purified from strain P1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR-associated protein Cas7/Cst2/DevR family. Subtype I-a/Apern subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C99297">
        <property type="entry name" value="C99297"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YC7"/>
    <dbReference type="SMR" id="Q97YC7"/>
    <dbReference type="STRING" id="273057.SSO1399"/>
    <dbReference type="PaxDb" id="273057-SSO1399"/>
    <dbReference type="EnsemblBacteria" id="AAK41634">
        <property type="protein sequence ID" value="AAK41634"/>
        <property type="gene ID" value="SSO1399"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1399"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1415"/>
    <dbReference type="eggNOG" id="arCOG03617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054331_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YC7"/>
    <dbReference type="PhylomeDB" id="Q97YC7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002764">
        <property type="entry name" value="Cas7/Cst2/DevR_sub_I-a/Apern"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010154">
        <property type="entry name" value="CRISPR-assoc_Cas7/Cst2/DevR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052681">
        <property type="entry name" value="CRISPR-Cas7/Cst2/DevR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01875">
        <property type="entry name" value="cas_MJ0381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02583">
        <property type="entry name" value="DevR_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37459">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37459:SF1">
        <property type="entry name" value="CRISPR-ASSOCIATED PROTEIN CAS7_CST2_DEVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01905">
        <property type="entry name" value="DevR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000417886" description="CRISPR-associated protein Cas7/Csa2 1">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="21507944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="318" mass="35852" checksum="6A6C3E459D62BBBD" modified="2001-10-01" version="1">MQLEVNNMWISFSVRYLVNVEDLNNVESAGNYVRHRRAPLVFKDKDSYTVTYVPAVSGEMIAHGYQMNLVELALQRNLPVDSLAKQGILIKRGSDDKVHEGTKCTDEKGSDYELCVINEDIVEDVAGFMNPNKLVKRTSNVAFSYMVPAIDAVKASTISSQFHVRYANKELMDKYKNENIQSLYNIETASASYVLTGYLNVNSVGKTQNYPVKEVDKKKDREKAALDALMLTLTQFLFGAKLTRFKPIVEIEALFVSASEKPFNLPPVTGDIKKYIDLVNSTTDSFAKILNIKRPVVKYYLKEEKGNVNTPIDAFTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YC9</accession>
    <name>Q97YC9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1397</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99297">
        <property type="entry name" value="A99297"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988389.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YC9"/>
    <dbReference type="SMR" id="Q97YC9"/>
    <dbReference type="STRING" id="273057.SSO1397"/>
    <dbReference type="PaxDb" id="273057-SSO1397"/>
    <dbReference type="DNASU" id="1454411"/>
    <dbReference type="EnsemblBacteria" id="AAK41632">
        <property type="protein sequence ID" value="AAK41632"/>
        <property type="gene ID" value="SSO1397"/>
    </dbReference>
    <dbReference type="GeneID" id="1454411"/>
    <dbReference type="KEGG" id="sso:SSO1397"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1413"/>
    <dbReference type="eggNOG" id="arCOG03847">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514679_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YC9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040581">
        <property type="entry name" value="cas_Crn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="19775" checksum="019C5176774B4811" modified="2001-10-01" version="1">MVKLVATLGKSPGGIAETLDNLISGNYVAPFEAKQIKVNELVVIRTEEVTESYYFLKTILLCCLDFTNVKEVSLPFDDISFPQDFITVRETVRKVLSTGDYLDFSGGRKAITAAAVLTARDVGAHLVTTIIDQDDYIRMNKRYEELKGKALSVYNKGQCLSYFCDLMSSKAKTIIFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YD1</accession>
    <name>Q97YD1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Clan AA aspartic protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90296">
        <property type="entry name" value="G90296"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YD1"/>
    <dbReference type="PaxDb" id="273057-SSO1395"/>
    <dbReference type="EnsemblBacteria" id="AAK41630">
        <property type="protein sequence ID" value="AAK41630"/>
        <property type="gene ID" value="SSO1395"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1395"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1410"/>
    <dbReference type="eggNOG" id="arCOG04029">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176719_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YD1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022274">
        <property type="entry name" value="Peptidase_asp_AF0612"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03698">
        <property type="entry name" value="clan_AA_DTGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="12753" checksum="3220FB322C928ED8" modified="2001-10-01" version="1">MKCRIGCISLDFELETEGIKVRAKVDTGFDGEIIVTKEIFDKIPYNSSDGTRICTASMECYSTFVKLVRTKFLGREITAEVLNSPVIEKNIIGEGLLKKVSAVINYKDNKIEDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YE6</accession>
    <name>Q97YE6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90294">
        <property type="entry name" value="G90294"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YE6"/>
    <dbReference type="STRING" id="273057.SSO1380"/>
    <dbReference type="PaxDb" id="273057-SSO1380"/>
    <dbReference type="EnsemblBacteria" id="AAK41614">
        <property type="protein sequence ID" value="AAK41614"/>
        <property type="gene ID" value="SSO1380"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1380"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1385"/>
    <dbReference type="eggNOG" id="arCOG07239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1056102_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YE6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="263" mass="31204" checksum="CE8B2E3919CEB7F7" modified="2001-10-01" version="1">MIGMEKKNSINISLLENLANFFSIGEIEKGELRANSEIINYLLEQGLIRQIERNFIVDELLKFIILKYSNIVKLKMSKDYLVKTQSIYILLMIVDENYHKIPFYDYIAYSGLVKIKKKRIARSVIRNGKKIINNNQGEIHLMKYQKNKIKAEINLDQTKSGEIIEFGLYIWNRPSKLSLMGDKTFSQNYFWIYAPTLEYSTKILLENNLNVESINLERFLYLDDNHQIKAPIKNQYMLRKEKNYIEIKVHYPDYGIYKLSYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YE8</accession>
    <name>Q97YE8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1378</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90294">
        <property type="entry name" value="E90294"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YE8"/>
    <dbReference type="STRING" id="273057.SSO1378"/>
    <dbReference type="PaxDb" id="273057-SSO1378"/>
    <dbReference type="EnsemblBacteria" id="AAK41612">
        <property type="protein sequence ID" value="AAK41612"/>
        <property type="gene ID" value="SSO1378"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1378"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1383"/>
    <dbReference type="eggNOG" id="arCOG12761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_408631_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YE8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="146"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="672" mass="76401" checksum="7BEA2F01E86FC2E0" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YL3</accession>
    <name>Q97YL3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF403 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90286">
        <property type="entry name" value="C90286"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923303.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YL3"/>
    <dbReference type="STRING" id="273057.SSO1310"/>
    <dbReference type="PaxDb" id="273057-SSO1310"/>
    <dbReference type="EnsemblBacteria" id="AAK41546">
        <property type="protein sequence ID" value="AAK41546"/>
        <property type="gene ID" value="SSO1310"/>
    </dbReference>
    <dbReference type="GeneID" id="1454325"/>
    <dbReference type="KEGG" id="sso:SSO1310"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1311"/>
    <dbReference type="eggNOG" id="arCOG07295">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1902036_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YL3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007296">
        <property type="entry name" value="DUF403"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04168">
        <property type="entry name" value="Alpha-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF403" evidence="1">
        <location>
            <begin position="6"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="133" mass="15692" checksum="B4748EDD650552E7" modified="2001-10-01" version="1">MLPKSIAYKIYWAGRYLERIENICRMSLLAINNGLNINTVAKQLGFDNEYELINYVKTSFQYLRENVRSFADEKVIIQVNTLEFLIDSDKSDLQSYFTQLLNGVYNVGNSFEKFFVEVRSEMRIRPQQENQPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YP2</accession>
    <name>Q97YP2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligo/dipeptide transport, permease protein (DppC-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dppC-2</name>
        <name evidence="7" type="ordered locus">SSO1283</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99283">
        <property type="entry name" value="A99283"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YP2"/>
    <dbReference type="FunCoup" id="Q97YP2">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1283"/>
    <dbReference type="PaxDb" id="273057-SSO1283"/>
    <dbReference type="EnsemblBacteria" id="AAK41520">
        <property type="protein sequence ID" value="AAK41520"/>
        <property type="gene ID" value="SSO1283"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1283"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1282"/>
    <dbReference type="eggNOG" id="arCOG00749">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_10_0_2"/>
    <dbReference type="InParanoid" id="Q97YP2"/>
    <dbReference type="PhylomeDB" id="Q97YP2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42729">
        <property type="entry name" value="OLIGO/DIPEPTIDE TRANSPORT, PERMEASE PROTEIN (DPPC-2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42729:SF1">
        <property type="entry name" value="OLIGO_DIPEPTIDE TRANSPORT, PERMEASE PROTEIN (DPPC-2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="329"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="365"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="499"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="327"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="536" mass="59323" checksum="134A43BD8C60D3EF" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YW0</accession>
    <name>Q97YW0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable thymidylate kinase</fullName>
            <ecNumber evidence="7">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tmk</name>
        <name evidence="9" type="ordered locus">SSO1191</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>dTMP + ATP = dTDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90273">
        <property type="entry name" value="A90273"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923237.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YW0"/>
    <dbReference type="FunCoup" id="Q97YW0">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1191"/>
    <dbReference type="PaxDb" id="273057-SSO1191"/>
    <dbReference type="DNASU" id="1454219"/>
    <dbReference type="EnsemblBacteria" id="AAK41440">
        <property type="protein sequence ID" value="AAK41440"/>
        <property type="gene ID" value="SSO1191"/>
    </dbReference>
    <dbReference type="GeneID" id="1454219"/>
    <dbReference type="KEGG" id="sso:SSO1191"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1191"/>
    <dbReference type="eggNOG" id="arCOG01891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049131_1_1_2"/>
    <dbReference type="InParanoid" id="Q97YW0"/>
    <dbReference type="PhylomeDB" id="Q97YW0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004389">
        <property type="entry name" value="Probable thymidylate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF1">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate kinase-like" evidence="8">
        <location>
            <begin position="8"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="208" mass="23768" checksum="1D2BF4961FD3A9FC" modified="2001-10-01" version="1">MNILIIAFEGIEGSGKTSHLEATKKYLEKEGYGVVTFGLQMSKLIGDRIAQVKRNIIFERRTLFLAYVTDLADQIENYVKPSIDSGFIALADGYTLTLTSWGLTRGLEKEWIDDVLSMLPKPSISLPLISTPDEIVRRIIKKRGYLDPLETGIDICIKEDTFEAYIDYINRFQEALMSISSRENIIYTDKEFDEVQKEIVRRIDNITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YW6</accession>
    <name>Q97YW6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF973 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90272">
        <property type="entry name" value="C90272"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988684.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YW6"/>
    <dbReference type="PaxDb" id="273057-SSO1185"/>
    <dbReference type="EnsemblBacteria" id="AAK41434">
        <property type="protein sequence ID" value="AAK41434"/>
        <property type="gene ID" value="SSO1185"/>
    </dbReference>
    <dbReference type="GeneID" id="1454213"/>
    <dbReference type="KEGG" id="sso:SSO1185"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1182"/>
    <dbReference type="eggNOG" id="arCOG03768">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_851576_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YW6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009321">
        <property type="entry name" value="DUF973"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06157">
        <property type="entry name" value="DUF973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="35348" checksum="6DEA7261E8D24A64" modified="2001-10-01" version="1">MNYTEAEYKGLSYLRRGNIEIIIIILIELIAILLTYIMQSLGLPNPLQDFLILLPSSAISALLVILAYLDIQRGLSNLAKAEINIGINNVISFLFLVLSILAIIGVVSYTLSLVLTSVTSSILASLQTLLEIFAFITLILLGFSYKLIGDHYSNNYLSIGGLVLILGVILFIFKLEYGGLLAFIGLIITYIGLTNLLTIKKPVSPPPPPLQTPSAPSETTSSTPSAETTSTPPTVNIISHGGILRSNGEAFIAVSSDIEDQILSSTILNTDYSTNEITPKTLSKGYNSIKIKFNINPQQFTSGNNYTIRLVLASGKNIDVTTFFQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z03</accession>
    <name>Q97Z03_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transporter, ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1144</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90267">
        <property type="entry name" value="D90267"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923208.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z03"/>
    <dbReference type="FunCoup" id="Q97Z03">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1144"/>
    <dbReference type="PaxDb" id="273057-SSO1144"/>
    <dbReference type="EnsemblBacteria" id="AAK41395">
        <property type="protein sequence ID" value="AAK41395"/>
        <property type="gene ID" value="SSO1144"/>
    </dbReference>
    <dbReference type="GeneID" id="15297457"/>
    <dbReference type="KEGG" id="sso:SSO1144"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1135"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q97Z03"/>
    <dbReference type="PhylomeDB" id="Q97Z03"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43038:SF8">
        <property type="entry name" value="ABC-TYPE MULTIDRUG TRANSPORT SYSTEM, ATPASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43038">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY H, MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="4"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="275" mass="31650" checksum="D226FDB4F931D8A7" modified="2001-10-01" version="1">MYVIEVNNVWKAYGKIIANEDITMRVKEGEIVALLGPNGAGKTTLVKQIYGELTPTKGEIRVLGKKPTDRHVKKFLGVIPQECEPYGDLTVWDNIYYMGRLKGASKDEIKKRGEELLERLDLRSKRNTLARDLSGGLKRRTLIAMALINNPKLLILDEPTTGLDPEARREVWEILLNMRKEGQSMLLTTHYLDEAERLADKIYFLSRKIIVEGTPTQIKEKFADWYEVIDYTNGKVYKVKGEEELKKIIMTINGKFEVRMPSLEEIYLQVMKDVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z43</accession>
    <name>FAU1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable ribonuclease FAU-1</fullName>
            <ecNumber evidence="1">3.1.26.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA-binding protein FAU-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fau-1</name>
        <name type="ordered locus">SSO1093</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable RNase involved in rRNA stability through maturation and/or degradation of precursor rRNAs. Binds to RNA in loop regions with AU-rich sequences.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAU-1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99262">
        <property type="entry name" value="B99262"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989727.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z43"/>
    <dbReference type="SMR" id="Q97Z43"/>
    <dbReference type="STRING" id="273057.SSO1093"/>
    <dbReference type="PaxDb" id="273057-SSO1093"/>
    <dbReference type="EnsemblBacteria" id="AAK41353">
        <property type="protein sequence ID" value="AAK41353"/>
        <property type="gene ID" value="SSO1093"/>
    </dbReference>
    <dbReference type="GeneID" id="1454132"/>
    <dbReference type="KEGG" id="sso:SSO1093"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1091"/>
    <dbReference type="eggNOG" id="arCOG04307">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044303_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z43"/>
    <dbReference type="PhylomeDB" id="Q97Z43"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.380.10">
        <property type="entry name" value="FomD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01910">
        <property type="entry name" value="RNA_binding_AU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007295">
        <property type="entry name" value="DUF402"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035930">
        <property type="entry name" value="FomD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050212">
        <property type="entry name" value="Ntdp-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016730">
        <property type="entry name" value="RNA-bd_FAU-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39159">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39159:SF1">
        <property type="entry name" value="UPF0374 PROTEIN YGAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04167">
        <property type="entry name" value="DUF402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018644">
        <property type="entry name" value="RNA-binding_FAU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159234">
        <property type="entry name" value="FomD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <feature type="chain" id="PRO_0000334212" description="Probable ribonuclease FAU-1">
        <location>
            <begin position="1"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01910"/>
        </source>
    </evidence>
    <sequence length="424" mass="49257" checksum="54938B54AA4406A1" modified="2001-10-01" version="1">MKGRVRIRGIYATALTSIFSSLSYEIVQQSVEISERFMQEINNLSADITIKDFEDDRGKIIVMGNGIIEDDLRNVFKYSFHWRSPVKLYSVIEIDESCTYANFKVEPCLREGIVIKPPYDGKIILSETKAVSKYAIVWRGKGITTFSEHIVDEEEKMRLLTLSLPLNRKGYNVKWRSNAKYVALNELKEDLERLILRYENREFRDQGEDFYLITLSLPDKLYLDEVRKNVVDTVKYHHMLKLSYNREVDSLEKDKKGSLGKLLEGLISDFLKIEHIKADGKVIYLRGGKVIEKEVNDNGYRIVLRREFEGNGILDGIGKKIEEGDYDIVEYNSDKWYQIHKYYSGIDNSLKGVYINISTPPELLRGKIRYLDLEIDIAIRDSEIALLDEDELNKKSIYMPSSLVNKAKEVVNYLINRIQQNKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z58</accession>
    <name>Q97Z58_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1075</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90260">
        <property type="entry name" value="B90260"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z58"/>
    <dbReference type="FunCoup" id="Q97Z58">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1075"/>
    <dbReference type="PaxDb" id="273057-SSO1075"/>
    <dbReference type="EnsemblBacteria" id="AAK41337">
        <property type="protein sequence ID" value="AAK41337"/>
        <property type="gene ID" value="SSO1075"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1075"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1075"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_12_1_2"/>
    <dbReference type="InParanoid" id="Q97Z58"/>
    <dbReference type="PhylomeDB" id="Q97Z58"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="18"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="81"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="133" mass="14650" checksum="AF67BFF972BC57BA" modified="2001-10-01" version="1">MGSYLKSLYMLKRVKDFMSTPVFQVEANTSLQEVCKLMLERGVGSVVVTEQGIPKGIFTDRDAVKAIATSLSSSDEVRLAATMGNLIIVDEDIDVFEALKIMAANKIRHLPVKNKDGNIIGMFSITDVYKVYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z66</accession>
    <name>Q97Z66_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdenum transport protein ModA related protein (ModA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">modA</name>
        <name evidence="2" type="ordered locus">SSO1066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90259">
        <property type="entry name" value="B90259"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989883.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z66"/>
    <dbReference type="STRING" id="273057.SSO1066"/>
    <dbReference type="PaxDb" id="273057-SSO1066"/>
    <dbReference type="EnsemblBacteria" id="AAK41329">
        <property type="protein sequence ID" value="AAK41329"/>
        <property type="gene ID" value="SSO1066"/>
    </dbReference>
    <dbReference type="GeneID" id="1454108"/>
    <dbReference type="KEGG" id="sso:SSO1066"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1063"/>
    <dbReference type="eggNOG" id="arCOG00223">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125440_2_1_2"/>
    <dbReference type="InParanoid" id="Q97Z66"/>
    <dbReference type="PhylomeDB" id="Q97Z66"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051815">
        <property type="entry name" value="Molybdate_resp_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30432:SF1">
        <property type="entry name" value="DNA-BINDING TRANSCRIPTIONAL DUAL REGULATOR MODE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30432">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MODE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="75"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="118" mass="13338" checksum="21A6D35944F024A1" modified="2001-10-01" version="1">MRFRFKIWIEAEDGKPLIGKGGVKLLRAIEETGSLSSASKSLGVSYKFAWEYVKRINEILSDSVEMRKGGKNAGGSKVNEKLDKLLNIYEEAQKEISEILEKYNKKINEVLNQEGEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z75</accession>
    <name>Q97Z75_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">SSO1054</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90257">
        <property type="entry name" value="G90257"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989165.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z75"/>
    <dbReference type="FunCoup" id="Q97Z75">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1054"/>
    <dbReference type="PaxDb" id="273057-SSO1054"/>
    <dbReference type="EnsemblBacteria" id="AAK41318">
        <property type="protein sequence ID" value="AAK41318"/>
        <property type="gene ID" value="SSO1054"/>
    </dbReference>
    <dbReference type="GeneID" id="1454097"/>
    <dbReference type="KEGG" id="sso:SSO1054"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1050"/>
    <dbReference type="eggNOG" id="arCOG04397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_33_4_2"/>
    <dbReference type="InParanoid" id="Q97Z75"/>
    <dbReference type="PhylomeDB" id="Q97Z75"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072488">
        <property type="term" value="P:ammonium transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000020">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF10">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="424"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="10">
        <location>
            <begin position="69"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
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            <end position="509"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK41318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="509" mass="54110" checksum="921306D2362BA48A" modified="2001-10-01" version="1">MKSWKFKALLIGQVILVMLLFSNILAHSQTTTNSSALQQLNQSVQSILNRTSSYPAAAVPSWLDTGSNAWMLTAATFVGLQSVPGVALYYAGLSKKKYAVNSALMIFYAFAAVLVIWIIAGYSFGFGYPALVSIHGYGIFGYPIPAWGGLFEASQTTYGPSGAHANIPTATFIFFQFVFAAITPILLAGGVLERMNFKAWMVFVPFWSLLVYSPVAYWLFAGGWLNQLGAVDFSGGYVIHVDAGVGALAAALAIGPRLASERKLEAHSLPLVLVGAGLIWLGWDGFNGGDPLGATVDAAIAVLNTNIATAVSAVVWMLMDMKFFGKPTLIGATSGAITGLVAITPAAGYVNGWEAALIGIFSGSIPWMALYWLEPKLRVDDTLGVFSTHGIAGIVGGLLTGVFANPAVTQFVAPGLTGALYGNWYQLGIQALAAVIVFVYDFAITFGLLKFIGLFIPLQAPPQELAIGDYAMHGEVAYSELLATIPESAKSKEQSVVLPKKEKEENSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZA6</accession>
    <name>Q97ZA6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NADH-Ubiquinone/plastoquinone related</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO1024</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90253">
        <property type="entry name" value="H90253"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="Q97ZA6">
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    </dbReference>
    <dbReference type="STRING" id="273057.SSO1024"/>
    <dbReference type="PaxDb" id="273057-SSO1024"/>
    <dbReference type="DNASU" id="1455254"/>
    <dbReference type="EnsemblBacteria" id="AAK41287">
        <property type="protein sequence ID" value="AAK41287"/>
        <property type="gene ID" value="SSO1024"/>
    </dbReference>
    <dbReference type="GeneID" id="1455254"/>
    <dbReference type="KEGG" id="sso:SSO1024"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1015"/>
    <dbReference type="eggNOG" id="arCOG01537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_10_2_2"/>
    <dbReference type="InParanoid" id="Q97ZA6"/>
    <dbReference type="PhylomeDB" id="Q97ZA6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="ComplexI-like_HydComp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
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        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005042">
        <property type="entry name" value="PRK06458.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HYDROGENASE-4 COMPONENT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42682:SF5">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="345"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="416"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="8">
        <location>
            <begin position="112"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="440" mass="48653" checksum="03D8D84117630A96" modified="2001-10-01" version="1">MNIEPILLLLIPIISNVGFFKLKLIKTLSVLSAVLTSIISIFLYFLAPIKNSFFFITKFTTFFLLVIASIYLLSTLYSMNYIKPSKIVSERLYYILLNSFASSMLFTVIMNNYGLMWVGVELTTVTSALLIIAEASETSLEATWRYIIIVSAGVTLALFSIIFIYYNYHTLTVTEILTKPENNIITKLAVALALIGFGTKAGVFPMYTWLPDAHSEAPSPISALFSGVLLPAATYVVYMVYQVNPLTNIFVIFTTLSIITASIILTYQWHIKRMFAYSTIENMNLALLGLTIGQPLGAIILLLAHAFGKAGAFYSSGIVLKVLGEKRIENIGGLHTKLKLTSVSLLLSSLAVTGTPPFATFIGEFFILQTLIQKGYIIEFILIVISLATAFISINYNVTKMIFTQRELTVSEEPKLITFISLVSSIIPLVLGILLLVILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZE5</accession>
    <name>Q97ZE5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">3-hydroxyisobutyrate dehydrogenase</fullName>
            <ecNumber evidence="5">1.1.1.31</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO0974</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.31" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90248">
        <property type="entry name" value="H90248"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992423.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZE5"/>
    <dbReference type="FunCoup" id="Q97ZE5">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0974"/>
    <dbReference type="PaxDb" id="273057-SSO0974"/>
    <dbReference type="EnsemblBacteria" id="AAK41247">
        <property type="protein sequence ID" value="AAK41247"/>
        <property type="gene ID" value="SSO0974"/>
    </dbReference>
    <dbReference type="GeneID" id="1455214"/>
    <dbReference type="KEGG" id="sso:SSO0974"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.972"/>
    <dbReference type="eggNOG" id="arCOG00247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035117_0_6_2"/>
    <dbReference type="InParanoid" id="Q97ZE5"/>
    <dbReference type="PhylomeDB" id="Q97ZE5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008442">
        <property type="term" value="F:3-hydroxyisobutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981:SF80">
        <property type="entry name" value="BLR4309 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="3">
        <location>
            <begin position="2"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyisobutyrate dehydrogenase-like NAD-binding" evidence="4">
        <location>
            <begin position="161"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="289" mass="31658" checksum="771D8F3B8AC61519" modified="2001-10-01" version="1">MKIGLIGLGIMGYRIAANLAKANKLNLVYDRTQEKIESFVKEYKVNGVQNTKDLVESSDVIITMLADDYAVKSVVEPLIPLMKGKILIDMSTISPTLSISLAKRIESNGGTMFDAPVIGTSVFVEQKKLVVLVGGPKDKFDIVNDIAKETASLVVYMGPNGMGLYSKLVNNLLLASYVAAIAEAYNFGIRAGLEPQQVVNILTTLSSARSPTTELKAPKLLKEDYSTQFATKHMRKDLEIIMREAQNLKIITPVSSLVLELYKMAEALGYSEADFISVVEVFKKLSPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="148" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZF3</accession>
    <name>Q97ZF3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ATP-dependent helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO0965</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the Lhr helicase family. Lhr-Core subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90247">
        <property type="entry name" value="H90247"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992411.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZF3"/>
    <dbReference type="FunCoup" id="Q97ZF3">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0965"/>
    <dbReference type="PaxDb" id="273057-SSO0965"/>
    <dbReference type="EnsemblBacteria" id="AAK41239">
        <property type="protein sequence ID" value="AAK41239"/>
        <property type="gene ID" value="SSO0965"/>
    </dbReference>
    <dbReference type="GeneID" id="1455206"/>
    <dbReference type="KEGG" id="sso:SSO0965"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.963"/>
    <dbReference type="eggNOG" id="arCOG00557">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002025_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZF3"/>
    <dbReference type="PhylomeDB" id="Q97ZF3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052511">
        <property type="entry name" value="ATP-dep_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017170">
        <property type="entry name" value="Lhr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962:SF5">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037307">
        <property type="entry name" value="Lhr-like_helic_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="29"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="240"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="786"/>
            <end position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00093467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="933" mass="108519" checksum="AAC24E50447E7F80" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZH6</accession>
    <name>GATE_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit E</fullName>
            <shortName evidence="1">Glu-ADT subunit E</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatE</name>
        <name type="ordered locus">SSO0936</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatB/GatE family. GatE subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90244">
        <property type="entry name" value="E90244"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992367.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZH6"/>
    <dbReference type="SMR" id="Q97ZH6"/>
    <dbReference type="FunCoup" id="Q97ZH6">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0936"/>
    <dbReference type="PaxDb" id="273057-SSO0936"/>
    <dbReference type="EnsemblBacteria" id="AAK41212">
        <property type="protein sequence ID" value="AAK41212"/>
        <property type="gene ID" value="SSO0936"/>
    </dbReference>
    <dbReference type="GeneID" id="1455179"/>
    <dbReference type="GeneID" id="44129865"/>
    <dbReference type="KEGG" id="sso:SSO0936"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.932"/>
    <dbReference type="eggNOG" id="arCOG01719">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030702_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZH6"/>
    <dbReference type="PhylomeDB" id="Q97ZH6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.380:FF:000002">
        <property type="entry name" value="Glutamyl-tRNA(Gln) amidotransferase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.30:FF:000003">
        <property type="entry name" value="Glutamyl-tRNA(Gln) amidotransferase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="entry name" value="GatB domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00588">
        <property type="entry name" value="GatE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004414">
        <property type="entry name" value="GatE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00134">
        <property type="entry name" value="gatE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003107">
        <property type="entry name" value="PRK04028.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT B MITOCHONDRIAL AND PROKARYOTIC PET112-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF2">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="GAD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140082" description="Glutamyl-tRNA(Gln) amidotransferase subunit E">
        <location>
            <begin position="1"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="415"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="633" mass="71534" checksum="C168309831E2EE01" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZI1</accession>
    <name>Q97ZI1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe-S cluster assembly protein SufB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0927</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-sulfur cluster assembly SufBD family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90243">
        <property type="entry name" value="H90243"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992359.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZI1"/>
    <dbReference type="FunCoup" id="Q97ZI1">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0927"/>
    <dbReference type="PaxDb" id="273057-SSO0927"/>
    <dbReference type="EnsemblBacteria" id="AAK41207">
        <property type="protein sequence ID" value="AAK41207"/>
        <property type="gene ID" value="SSO0927"/>
    </dbReference>
    <dbReference type="GeneID" id="1455174"/>
    <dbReference type="KEGG" id="sso:SSO0927"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.927"/>
    <dbReference type="eggNOG" id="arCOG01715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026231_0_1_2"/>
    <dbReference type="InParanoid" id="Q97ZI1"/>
    <dbReference type="PhylomeDB" id="Q97ZI1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990229">
        <property type="term" value="C:iron-sulfur cluster assembly complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010231">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01980">
        <property type="entry name" value="sufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508">
        <property type="entry name" value="FES CLUSTER ASSEMBLY PROTEIN SUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508:SF1">
        <property type="entry name" value="UPF0051 PROTEIN ABCI8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD N-terminal" evidence="3">
        <location>
            <begin position="145"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="2">
        <location>
            <begin position="214"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="479" mass="54177" checksum="DE639952CE685E71" modified="2001-10-01" version="1">MQEDKISLDLHEIINATIDAKYNKLNQLEFHRRIVESGLSRSTIEEISRIKKEPEWMLKLRLKGLELFEKLPTPNWLPDVLSSLDISALELYVKPGVDKAQSWEELPPEIRKYYDELGIPESEKKFLGGLVAVLESEPIYSNVKIELMKKGVVMLPIDEAVSKYPDLMKEYFMKIFPASDHKFAALHGALWSGGVFVYVPKNVKITTPVEGFFVIGSELEGQFEHTLLIADEGSYIHFIEGCTAPQLKKYSFHDGMVELYAKKNAYIKFTTIQNWSKNVINFNNKRAWADENSTVEWVEGSLGSKYSFVYPSTILRGKNASSTSLVVTMTSGEGEWKDSGSKMIHAAPYTKSKVVNKNIGFNGGVNVYRGLVKVNKGAVGSKAFVKCDSLMLDDKTKAYTYPHNQVLEEDADVGHEAHTFRMNEDQLFYLMTRGIDEKEATSMLVLGFIDEIMKELPFEYATMLNKVIKLELDKLGAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZQ0</accession>
    <name>Q97ZQ0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative snRNP Sm-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">snRNP-2</name>
        <name evidence="5" type="ordered locus">SSO6454</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2005" name="Proteins" volume="61" first="689" last="693">
            <title>Crystal structure of an archaeal Sm protein from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Kilic T."/>
                <person name="Thore S."/>
                <person name="Suck D."/>
            </authorList>
            <dbReference type="PubMed" id="16184597"/>
            <dbReference type="DOI" id="10.1002/prot.20637"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.68 ANGSTROMS)</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90224">
        <property type="entry name" value="F90224"/>
    </dbReference>
    <dbReference type="PDB" id="1TH7">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.68 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L/M/N=1-79"/>
    </dbReference>
    <dbReference type="PDBsum" id="1TH7"/>
    <dbReference type="AlphaFoldDB" id="Q97ZQ0"/>
    <dbReference type="SMR" id="Q97ZQ0"/>
    <dbReference type="FunCoup" id="Q97ZQ0">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO6454"/>
    <dbReference type="PaxDb" id="273057-SSO6454"/>
    <dbReference type="EnsemblBacteria" id="AAK41053">
        <property type="protein sequence ID" value="AAK41053"/>
        <property type="gene ID" value="SSO6454"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO6454"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.755"/>
    <dbReference type="eggNOG" id="arCOG00998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_11_1_2"/>
    <dbReference type="InParanoid" id="Q97ZQ0"/>
    <dbReference type="PhylomeDB" id="Q97ZQ0"/>
    <dbReference type="EvolutionaryTrace" id="Q97ZQ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01731">
        <property type="entry name" value="archaeal_Sm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022901">
        <property type="entry name" value="snRNP_Sm-like_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF5">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="7" id="KW-0002">3D-structure</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="4">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="1TH7"/>
        </source>
    </evidence>
    <sequence length="79" mass="8679" checksum="5886E9444EF65F62" modified="2001-10-01" version="1">MNFLAETAHKVLAESLNNLVLVKLKGNKEVRGMLRSYDQHMNLVLSDSEEIQSDGSGKKLGTIVIRGDNVILISPLQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZQ2</accession>
    <name>Q97ZQ2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0754</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90224">
        <property type="entry name" value="B90224"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZQ2"/>
    <dbReference type="PaxDb" id="273057-SSO0754"/>
    <dbReference type="EnsemblBacteria" id="AAK41049">
        <property type="protein sequence ID" value="AAK41049"/>
        <property type="gene ID" value="SSO0754"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0754"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.751"/>
    <dbReference type="eggNOG" id="arCOG07720">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1127133_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZQ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="246" mass="26700" checksum="48F1DB5E7F487AF3" modified="2001-10-01" version="1">MISLKEYYKYKREGNKKMYEIYPAIAVAERILAVAVSNSSSAYELKTKIFRQGIVIRTEDTGEYYYIGGISPVWIGGSNIAVYQGGAAFRGLVKLPAKARDIFISNGEIGIIPLKRVSAQIEWYNPARFKSCQGLLGAIWDYITTVGVALVVAIASLASEITLSSPRSIDDLVYPGSSGSYYVSKTLIGSSLGAKSSDELRTISDQYPFIYLALASFKQVASGLGLSNYLAVTVNGPIDVVLNNCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZS7</accession>
    <name>Q97ZS7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Primase X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0502</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2015" name="Nat. Commun." volume="6" first="7300" last="7300">
            <title>A primase subunit essential for efficient primer synthesis by an archaeal eukaryotic-type primase.</title>
            <authorList>
                <person name="Liu B."/>
                <person name="Ouyang S."/>
                <person name="Makarova K.S."/>
                <person name="Xia Q."/>
                <person name="Zhu Y."/>
                <person name="Li Z."/>
                <person name="Guo L."/>
                <person name="Koonin E.V."/>
                <person name="Liu Z.J."/>
                <person name="Huang L."/>
            </authorList>
            <dbReference type="PubMed" id="26095544"/>
            <dbReference type="DOI" id="10.1038/ncomms8300"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 26-154</scope>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2017" name="Nat. Commun." volume="8" first="1718" last="1718">
            <title>Primer synthesis by a eukaryotic-like archaeal primase is independent of its Fe-S cluster.</title>
            <authorList>
                <person name="Holzer S."/>
                <person name="Yan J."/>
                <person name="Kilkenny M.L."/>
                <person name="Bell S.D."/>
                <person name="Pellegrini L."/>
            </authorList>
            <dbReference type="PubMed" id="29167441"/>
            <dbReference type="DOI" id="10.1038/s41467-017-01707-w"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.91 ANGSTROMS) IN COMPLEX WITH MN(2+)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90195">
        <property type="entry name" value="F90195"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991394.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="4WYH">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.95 A"/>
        <property type="chains" value="A=26-154"/>
    </dbReference>
    <dbReference type="PDB" id="5OF3">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.91 A"/>
        <property type="chains" value="C/F=1-154"/>
    </dbReference>
    <dbReference type="PDB" id="5OFN">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="B/C=42-154"/>
    </dbReference>
    <dbReference type="PDBsum" id="4WYH"/>
    <dbReference type="PDBsum" id="5OF3"/>
    <dbReference type="PDBsum" id="5OFN"/>
    <dbReference type="AlphaFoldDB" id="Q97ZS7"/>
    <dbReference type="SMR" id="Q97ZS7"/>
    <dbReference type="STRING" id="273057.SSO0502"/>
    <dbReference type="PaxDb" id="273057-SSO0502"/>
    <dbReference type="EnsemblBacteria" id="AAK40821">
        <property type="protein sequence ID" value="AAK40821"/>
        <property type="gene ID" value="SSO0502"/>
    </dbReference>
    <dbReference type="GeneID" id="1455631"/>
    <dbReference type="KEGG" id="sso:SSO0502"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.495"/>
    <dbReference type="eggNOG" id="arCOG05924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118423_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZS7"/>
    <dbReference type="BRENDA" id="2.7.7.102">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="2.7.7.B16">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97ZS7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040865">
        <property type="entry name" value="PriX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033412">
        <property type="entry name" value="primase_PriX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18689">
        <property type="entry name" value="PriX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4 5 6" id="KW-0002">3D-structure</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Primase X" evidence="1">
        <location>
            <begin position="57"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="4WYH"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="5OF3"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="5OFN"/>
        </source>
    </evidence>
    <sequence length="154" mass="18029" checksum="FEAC6CD7ACBC03A9" modified="2001-10-01" version="1">MSQEKKAKKIILHYPDDTPAGYIEYAEGSSSIYDNEGNFLFKVEGKFPPQPKKSSDYSWIEKVLEMGLQDSRKRFILYVASRYLVNVKGVNEDEALQTLKEFYYKLQSGKVYESWLKSVINGVKKKGLLPWSLKRIEERDKEMYNEIIRVLKNS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZY8</accession>
    <accession>E0A3F4</accession>
    <name>KAE1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N6-L-threonylcarbamoyladenine synthase</fullName>
            <shortName evidence="1">t(6)A synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">kae1</name>
        <name type="ordered locus">SSO0434</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2010-05" db="EMBL/GenBank/DDBJ databases">
            <title>An update of the SSO5844-SSO0434-SSO0433 genomic region of the archaea Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Hecker A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="2010-05" db="EMBL/GenBank/DDBJ databases">
            <title>An update of the SSO0434 gene sequence from the archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Hecker A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="frameshift">
            <sequence resource="EMBL-CDS" id="AAK40758" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EMBL" id="HM366920">
        <property type="protein sequence ID" value="ADK39020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="HM370296">
        <property type="protein sequence ID" value="ADK88910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40758.1"/>
        <property type="status" value="ALT_FRAME"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90187">
        <property type="entry name" value="G90187"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZY8"/>
    <dbReference type="SMR" id="Q97ZY8"/>
    <dbReference type="FunCoup" id="Q97ZY8">
        <property type="interactions" value="177"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0434"/>
    <dbReference type="PaxDb" id="273057-SSO0434"/>
    <dbReference type="DNASU" id="1455568"/>
    <dbReference type="EnsemblBacteria" id="AAK40758">
        <property type="protein sequence ID" value="AAK40758"/>
        <property type="gene ID" value="SSO0434"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0434"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.426"/>
    <dbReference type="eggNOG" id="arCOG01183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023208_2_2_2"/>
    <dbReference type="InParanoid" id="Q97ZY8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000229">
        <property type="entry name" value="tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01446">
        <property type="entry name" value="Kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034680">
        <property type="entry name" value="Kae1_archaea_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03722">
        <property type="entry name" value="arch_KAE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF14">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000303645" description="tRNA N6-adenosine threonylcarbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="130"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="331" mass="36290" checksum="F89341F2AF0C6BD1" modified="2013-12-11" version="2">MFVLGIESTAHTFGVGIVRDSPPYILANERDTFIPKEGGMKPGDLLKHHAEVSATILRRALEKAKISINDINYIAVALGPGIGPALRVGATLARAIALKYNKKLVPVNHGIGHIEIGYLTTEARDPLILYLSGGNTIITTFYKGRFRVFGETLDIALGNMMDVFVREVSLAPPYIINGIHVIDICAEKGNKLLKLPYVVKGQDMSFSGLLTAALRVVGKEKLEDICYSVREIAFDMLLEATERALALTSKKELMIVGGVAASVSLRKKLEELGKEWNVQIKIVPPEFAGDNGAMIAYAGMLAASKGVFIDVDKSYIRPRWRVDEVDIPWRN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-12-13" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q980B6</accession>
    <name>GCH3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTP cyclohydrolase III</fullName>
            <ecNumber evidence="1">3.5.4.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gch3</name>
        <name type="ordered locus">SSO0399</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of 2-amino-5-formylamino-6-ribofuranosylamino-4(3H)-pyrimidinone ribonucleotide monophosphate and inorganic phosphate from GTP. Also has an independent pyrophosphate phosphohydrolase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + 3 H2O = 2-amino-5-formylamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57258"/>
            <dbReference type="EC" id="3.5.4.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal-type GTP cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.29" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99184">
        <property type="entry name" value="A99184"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988791.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980B6"/>
    <dbReference type="SMR" id="Q980B6"/>
    <dbReference type="FunCoup" id="Q980B6">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0399"/>
    <dbReference type="PaxDb" id="273057-SSO0399"/>
    <dbReference type="EnsemblBacteria" id="AAK40728">
        <property type="protein sequence ID" value="AAK40728"/>
        <property type="gene ID" value="SSO0399"/>
    </dbReference>
    <dbReference type="GeneID" id="1455538"/>
    <dbReference type="KEGG" id="sso:SSO0399"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.395"/>
    <dbReference type="eggNOG" id="arCOG04202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080076_0_0_2"/>
    <dbReference type="InParanoid" id="Q980B6"/>
    <dbReference type="PhylomeDB" id="Q980B6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043740">
        <property type="term" value="F:GTP cyclohydrolase IIa activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00608">
        <property type="entry name" value="GTP_cyclohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007839">
        <property type="entry name" value="GTP_CycHdrlase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42202">
        <property type="entry name" value="GTP CYCLOHYDROLASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42202:SF1">
        <property type="entry name" value="GTP CYCLOHYDROLASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05165">
        <property type="entry name" value="GCH_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009265">
        <property type="entry name" value="GTP_cyclohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000145758" description="GTP cyclohydrolase III">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00608"/>
        </source>
    </evidence>
    <sequence length="231" mass="27013" checksum="5119B366CD40AB18" modified="2001-10-01" version="1">MKILAIKLLDYREWTERLGYDREWLIQKIQNKFMMKIHEIASQYNTFPLQLRFDNFLMIADGITTTQLAYMINDMQENLPVRIKSCLGYGKTPLEAQWNASICLNNQDDNEIKEYTDEKIAALHFDINFNTETLKYTSVYDSFIEITNIYVNLSRFLYKIGGILQYLGGDNYLGFISTESVNKVIEEFSDDNKIKVGIGIGKNARTAIRLATTSLEKIRNNREKTWHMEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q980G7</accession>
    <name>Q980G7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myo-inositol-1-phosphate synthase GAPDH-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the myo-inositol 1-phosphate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90176">
        <property type="entry name" value="B90176"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990624.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980G7"/>
    <dbReference type="FunCoup" id="Q980G7">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0330"/>
    <dbReference type="PaxDb" id="273057-SSO0330"/>
    <dbReference type="EnsemblBacteria" id="AAK40665">
        <property type="protein sequence ID" value="AAK40665"/>
        <property type="gene ID" value="SSO0330"/>
    </dbReference>
    <dbReference type="GeneID" id="1455475"/>
    <dbReference type="KEGG" id="sso:SSO0330"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.325"/>
    <dbReference type="eggNOG" id="arCOG04213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050011_0_0_2"/>
    <dbReference type="InParanoid" id="Q980G7"/>
    <dbReference type="PhylomeDB" id="Q980G7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052199">
        <property type="entry name" value="MIPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013021">
        <property type="entry name" value="Myo-inos-1-P_Synthase_GAPDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43125">
        <property type="entry name" value="INOSITOL-3-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43125:SF1">
        <property type="entry name" value="INOSITOL-3-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01658">
        <property type="entry name" value="Inos-1-P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07994">
        <property type="entry name" value="NAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015578">
        <property type="entry name" value="Myoinos-ppht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myo-inositol-1-phosphate synthase GAPDH-like" evidence="2">
        <location>
            <begin position="180"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="344" mass="37935" checksum="8CAF02B7ED069F69" modified="2001-10-01" version="1">MIRIALIGVGNVASALVQSIELIRNGKEIYGILDLPIRPNDIEIVAAFDIDKRKVNKRLREAIFTKPNVVDKYVDVKNDIIVMRGPTLDGRVGILSNIIEESEEEPVDIVDVLKKEKVDVVVNLLPTGATSASNFYAKASLEAGSAFINTTPSPTAKEFKDQFMEKRLPILGDDLLSQIGGTITHAGIIEFLKSRGVKVLKSYQIDIAGSTEALVALEDWRKDLKKSLKSSFISSFTNDVEVVAGTSDYVEFLKDRRVSYMVIEGLYGIGVPIRIDISLKTYDSFNAVAPLIDLIRIAKLLGRHGIGGAVKEVCYYYFKDPPEKAKSIMDAKRSLDSLLNKLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q980G8</accession>
    <name>Q980G8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH dehydrogenase subunit N (NuoN)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NuoN</name>
        <name evidence="7" type="ordered locus">SSO0329</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90176">
        <property type="entry name" value="A90176"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980G8"/>
    <dbReference type="FunCoup" id="Q980G8">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0329"/>
    <dbReference type="PaxDb" id="273057-SSO0329"/>
    <dbReference type="EnsemblBacteria" id="AAK40664">
        <property type="protein sequence ID" value="AAK40664"/>
        <property type="gene ID" value="SSO0329"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0329"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.324"/>
    <dbReference type="eggNOG" id="arCOG01540">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_1_5_2"/>
    <dbReference type="InParanoid" id="Q980G8"/>
    <dbReference type="PhylomeDB" id="Q980G8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004445">
        <property type="entry name" value="PRK05777.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22773">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="334"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="456"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="6">
        <location>
            <begin position="144"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="483" mass="53087" checksum="79A625B9585DA000" modified="2001-10-01" version="1">MGYSFTFNYSFDSIRGDWIMDPMYYLPILIPSIFILFSSISVLFIDNGLDENRFRLSVILSVVLLGVSLAFLIYSWTASVINYTLFSKSLLIDVPGYFFSITVFSIGILAILLSKDHIISWPTRSSMLSLMLLSLLGLFYMSFANNIIIVLTTWAISSAASYAIAMLRKDYKSVDAGIKYLIMGLLSSSIMIIGFASYLISTGSLLFTTSVIYPNLFFFSISFISIAFLFKIGAFPFQAWLPDVYTDADRISVAFISSVGKLIGIIPLFRVVYLSDPNMVELVIFIVISVASMLVGNITAFSRRDVAAILSFSSITQMGYILIGFAMFTVSPAIAVVGILVQSLAYGIAQVGLFGIVSHVEKVSGTSDISGLRGISSQDKPLAFAIVVLILSLLGIPPIFGFWGKLFLFESSFTYPWLTIIAVLNSAISAGYYVPIVREVFREGEFEVIKSSERNIAVVSAILSIILGIVIPIVFQILVSQID</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-13" modified="2026-01-28" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>Q980I9</accession>
    <name>AROB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-dehydroquinate synthase</fullName>
            <shortName evidence="1">DHQS</shortName>
            <ecNumber evidence="1">4.2.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroB</name>
        <name type="ordered locus">SSO0305</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3-dehydroquinate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21968"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="EC" id="4.2.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit. Can use either Co(2+) or Zn(2+).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.4" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90173">
        <property type="entry name" value="C90173"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990597.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980I9"/>
    <dbReference type="SMR" id="Q980I9"/>
    <dbReference type="FunCoup" id="Q980I9">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0305"/>
    <dbReference type="PaxDb" id="273057-SSO0305"/>
    <dbReference type="EnsemblBacteria" id="AAK40642">
        <property type="protein sequence ID" value="AAK40642"/>
        <property type="gene ID" value="SSO0305"/>
    </dbReference>
    <dbReference type="GeneID" id="1455452"/>
    <dbReference type="GeneID" id="44129278"/>
    <dbReference type="KEGG" id="sso:SSO0305"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.298"/>
    <dbReference type="eggNOG" id="arCOG00983">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001201_0_1_2"/>
    <dbReference type="InParanoid" id="Q980I9"/>
    <dbReference type="PhylomeDB" id="Q980I9"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00085"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08195">
        <property type="entry name" value="DHQS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1090.10:FF:000038">
        <property type="entry name" value="3-dehydroquinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00110">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050071">
        <property type="entry name" value="Dehydroquinate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016037">
        <property type="entry name" value="DHQ_synth_AroB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030963">
        <property type="entry name" value="DHQ_synth_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030960">
        <property type="entry name" value="DHQS/DOIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056179">
        <property type="entry name" value="DHQS_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01357">
        <property type="entry name" value="aroB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43622">
        <property type="entry name" value="3-DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43622:SF1">
        <property type="entry name" value="3-DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01761">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24621">
        <property type="entry name" value="DHQS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001455">
        <property type="entry name" value="DHQ_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000140822" description="3-dehydroquinate synthase">
        <location>
            <begin position="1"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="61"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="119"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="159"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00110"/>
        </source>
    </evidence>
    <sequence length="354" mass="39300" checksum="26DBD8C098DE41AB" modified="2001-10-01" version="1">MREILEDICCSEVRVVVGEGSLSKLSKIKDNNAAVIYSRKISIADKINKYLPNAYFIPINDGESTKELSSVISLVEKLFEKNFDRGDYIIGVGGGTVTDVAGFLASIYLRGLNLINVPTTFLGMVDAAIGGKNGVNFNNIKNLIGTFYQPSMIISDLEFLETLPIEELKKGLAEVIKYGLTLDKELYDYLSLNKEKILNKDKQALEDIIFRSTLDKLSIVKEDERETKGIRIVLNFGHTIGHAIEAGSSFNVPHGYAISVGMVCEAKMAEELGYAEEGVVEDVLWLLQIYGLPYDISQIDAPVDLKLALNAINMDKKHRKDVILIPFPTRIGSWKKVEVPLDTVKGFAEQCLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q980K3</accession>
    <name>Q980K3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Inorganic phosphate permease related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO0290</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90171">
        <property type="entry name" value="E90171"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990574.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980K3"/>
    <dbReference type="FunCoup" id="Q980K3">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0290"/>
    <dbReference type="PaxDb" id="273057-SSO0290"/>
    <dbReference type="EnsemblBacteria" id="AAK40628">
        <property type="protein sequence ID" value="AAK40628"/>
        <property type="gene ID" value="SSO0290"/>
    </dbReference>
    <dbReference type="GeneID" id="1455438"/>
    <dbReference type="KEGG" id="sso:SSO0290"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.283"/>
    <dbReference type="eggNOG" id="arCOG02683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_14_2"/>
    <dbReference type="InParanoid" id="Q980K3"/>
    <dbReference type="PhylomeDB" id="Q980K3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="395"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="15"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="451" mass="49434" checksum="8778DBC3289D2629" modified="2001-10-01" version="1">MGYNKPDFSSLEFKSVLTAGMGVFTDGYELYAISLVFYYIQKEFLLNGLEEGIVIASAYYGAAVSAILLGFLSDKLGRKFIYGLDVALMSLGILFQSISTNFAELVMSRIILGFGIGADYVLSPIITAENSNPKNRGKRMIVTFALLWGMGAVTAAFVEQILLLVNMENDLIWRIVLFMGILPAISVFYLRRKIPETYKFLTRIKPKPEELKKLEKEIKSKVAISPDEVPFSKRFVSAIGITIISSILWLLYDMYSSTFAIYGPITIAGNLGLNPIEFTYLAQFLAGLPGQIISIYLVDKIGRKPLIVIGYAGVAFWLFMYSILLMDPYYFGLKIHISNPLKAAESLTGEAALLGLSFYLMNYLSSAIGPASIIGSAMLTPELVPTKVRGTSQAISVAVDRLAAAFSITAFPLLLAKFGLPVLLGIYSIIALVSCSITLFLIPETKGVELS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-03" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q980M4</accession>
    <name>TRM56_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (cytidine(56)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.206</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA ribose 2'-O-methyltransferase aTrm56</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0267</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically catalyzes the AdoMet-dependent 2'-O-ribose methylation of cytidine at position 56 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cytidine(56) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(56) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42968"/>
            <dbReference type="Rhea" id="RHEA-COMP:10308"/>
            <dbReference type="Rhea" id="RHEA-COMP:10309"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.206"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aTrm56 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.206" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90168">
        <property type="entry name" value="G90168"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990542.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980M4"/>
    <dbReference type="SMR" id="Q980M4"/>
    <dbReference type="STRING" id="273057.SSO0267"/>
    <dbReference type="PaxDb" id="273057-SSO0267"/>
    <dbReference type="EnsemblBacteria" id="AAK40606">
        <property type="protein sequence ID" value="AAK40606"/>
        <property type="gene ID" value="SSO0267"/>
    </dbReference>
    <dbReference type="GeneID" id="1455416"/>
    <dbReference type="KEGG" id="sso:SSO0267"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.262"/>
    <dbReference type="eggNOG" id="arCOG01857">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123709_0_0_2"/>
    <dbReference type="InParanoid" id="Q980M4"/>
    <dbReference type="PhylomeDB" id="Q980M4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106059">
        <property type="term" value="F:tRNA (cytidine(56)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00077">
        <property type="entry name" value="tRNA_methyltr_aTrm56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002845">
        <property type="entry name" value="tRNA_mtfrase_aTrm56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42197">
        <property type="entry name" value="TRNA (CYTIDINE(56)-2'-O)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42197:SF1">
        <property type="entry name" value="TRNA (CYTIDINE(56)-2'-O)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01994">
        <property type="entry name" value="Trm56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016123">
        <property type="entry name" value="UCP016123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000365321" description="tRNA (cytidine(56)-2'-O)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="112"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00077"/>
        </source>
    </evidence>
    <sequence length="160" mass="18185" checksum="9B5E819443D079F2" modified="2001-10-01" version="1">MTTHVILVARAFGAKGVYIEGKDEKMVKSILKVIDSWGGSSYFLVKEIENGKSIVNEWKEKGGTIIHLTMYGININDFQDRFEKIKYPLLIIVGAEKVEGWYYHNADYNIAIGNQPHSEVAALAIFLDRIYKGRELYMEFEDAKIKILPQKAGKKVIRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q980N6</accession>
    <name>Q980N6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO0254</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAM hydrolase / SAM-dependent halogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90167">
        <property type="entry name" value="B90167"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980N6"/>
    <dbReference type="STRING" id="273057.SSO0254"/>
    <dbReference type="PaxDb" id="273057-SSO0254"/>
    <dbReference type="EnsemblBacteria" id="AAK40593">
        <property type="protein sequence ID" value="AAK40593"/>
        <property type="gene ID" value="SSO0254"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0254"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.248"/>
    <dbReference type="eggNOG" id="arCOG04309">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059734_1_1_2"/>
    <dbReference type="InParanoid" id="Q980N6"/>
    <dbReference type="PhylomeDB" id="Q980N6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.90">
        <property type="entry name" value="Bacterial fluorinating enzyme like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10790">
        <property type="entry name" value="S-adenosyl-l-methionine hydroxide adenosyltransferase, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046470">
        <property type="entry name" value="SAM_HAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046469">
        <property type="entry name" value="SAM_HAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002747">
        <property type="entry name" value="SAM_OH_AdoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023227">
        <property type="entry name" value="SAM_OH_AdoTrfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023228">
        <property type="entry name" value="SAM_OH_AdoTrfase_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35092">
        <property type="entry name" value="CHLORINASE MJ1651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35092:SF1">
        <property type="entry name" value="CHLORINASE MJ1651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20257">
        <property type="entry name" value="SAM_HAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01887">
        <property type="entry name" value="SAM_HAT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006779">
        <property type="entry name" value="UCP006779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101852">
        <property type="entry name" value="Bacterial fluorinating enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102522">
        <property type="entry name" value="Bacterial fluorinating enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="S-adenosyl-l-methionine hydroxide adenosyltransferase N-terminal" evidence="3">
        <location>
            <begin position="11"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosyl-l-methionine hydroxide adenosyltransferase C-terminal" evidence="4">
        <location>
            <begin position="181"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="281" mass="31911" checksum="23B24338E3FF375F" modified="2001-10-01" version="1">MINMRRVPNQIAILTDFGLNDNYNGVMEAVIKKINPEAKITYISGNSKPFNLIAGAYLLYTSYKYFPRNTIFLVVIDPGVGTSRRPLIIKTKNYVFIGPDNGVLYPAITSEEIVKIIEIKNEKLYLAKTISNTFHGRDIFSVAAAFISIGVEIDTFGNQVRYEDLTKLNFTYKPSKSEICGKVLYIDHFGNVATSIPKEVLLNNFKYDENIEIKVNNSKHKCRFVKTFGYGMENELLIYFNGYFLVEIGINKGSAKDKLNVREGDEICLEGYIQEDFSHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q980P0</accession>
    <name>Q980P0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HIT-like protein, hypothetical (Hit)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hit</name>
        <name evidence="4" type="ordered locus">SSO0249</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90166">
        <property type="entry name" value="E90166"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990510.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980P0"/>
    <dbReference type="FunCoup" id="Q980P0">
        <property type="interactions" value="133"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0249"/>
    <dbReference type="PaxDb" id="273057-SSO0249"/>
    <dbReference type="EnsemblBacteria" id="AAK40588">
        <property type="protein sequence ID" value="AAK40588"/>
        <property type="gene ID" value="SSO0249"/>
    </dbReference>
    <dbReference type="GeneID" id="1455398"/>
    <dbReference type="KEGG" id="sso:SSO0249"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.243"/>
    <dbReference type="eggNOG" id="arCOG00419">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_1_2_2"/>
    <dbReference type="InParanoid" id="Q980P0"/>
    <dbReference type="PhylomeDB" id="Q980P0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008796">
        <property type="term" value="F:bis(5'-nucleosyl)-tetraphosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015967">
        <property type="term" value="P:diadenosine tetraphosphate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01275">
        <property type="entry name" value="FHIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052908">
        <property type="entry name" value="AP-4-A_phosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039383">
        <property type="entry name" value="FHIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42997:SF1">
        <property type="entry name" value="AP-4-A PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42997">
        <property type="entry name" value="HIT FAMILY HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="3">
        <location>
            <begin position="25"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2">
        <location>
            <begin position="120"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="171" mass="19593" checksum="351EA24115EDB6EF" modified="2001-10-01" version="1">MDILWAPWRAKYISETSKVKQDECLFCRVIKEENDEQNYIVYRGKHAFIILNAFPYNTAHVMVVPYRHVPSVELLSNDEALDIFTLSTIAIRAIRDIYAPDGFNIGVNIGRVAGAGIESHIHVHIVPRWNGDSNFMPVIFNTKVMPETLSDTFKKLNTKVNEIMKKPSRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Q2</accession>
    <name>Q980Q2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoribosyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0233</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90165">
        <property type="entry name" value="A90165"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990490.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Q2"/>
    <dbReference type="SMR" id="Q980Q2"/>
    <dbReference type="STRING" id="273057.SSO0233"/>
    <dbReference type="PaxDb" id="273057-SSO0233"/>
    <dbReference type="DNASU" id="1455386"/>
    <dbReference type="EnsemblBacteria" id="AAK40576">
        <property type="protein sequence ID" value="AAK40576"/>
        <property type="gene ID" value="SSO0233"/>
    </dbReference>
    <dbReference type="GeneID" id="1455386"/>
    <dbReference type="KEGG" id="sso:SSO0233"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.231"/>
    <dbReference type="eggNOG" id="arCOG00031">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086256_1_0_2"/>
    <dbReference type="InParanoid" id="Q980Q2"/>
    <dbReference type="PhylomeDB" id="Q980Q2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050118">
        <property type="entry name" value="Pur/Pyrimidine_PRTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43864">
        <property type="entry name" value="HYPOXANTHINE/GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43864:SF1">
        <property type="entry name" value="XANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="3">
        <location>
            <begin position="123"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="246" mass="28561" checksum="4492A811EEB46F6E" modified="2001-10-01" version="1">MEIFMLQSNQIKEKELRLRLFSVDILKELKNIYTYKELSKFFNIQESLICRYVNGRTIPSERQALEIVTRIKNKEFLYNFFIRKIRVYEDDFIDVSNLLFYPNLLKTFLELYLIKLNKVSDVTKIVGIASNGIPFATIVASILEKPLIISKKHKDSTQIQYIEENIRESNGVISTLYIRGDYISKKDKILIVDDVIRSGKTLLSLYNLIGKANASVVGALIIATNTDTWKDKFINKDINLMVLFKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-11-16" modified="2025-06-18" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q980R3</accession>
    <name>RL30E_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL30</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>50S ribosomal protein L30e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rpl30e</name>
        <name type="ordered locus">SSO0221</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90163">
        <property type="entry name" value="E90163"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980R3"/>
    <dbReference type="SMR" id="Q980R3"/>
    <dbReference type="FunCoup" id="Q980R3">
        <property type="interactions" value="199"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0221"/>
    <dbReference type="PaxDb" id="273057-SSO0221"/>
    <dbReference type="EnsemblBacteria" id="AAK40564">
        <property type="protein sequence ID" value="AAK40564"/>
        <property type="gene ID" value="SSO0221"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0221"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.219"/>
    <dbReference type="eggNOG" id="arCOG01752">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130502_1_0_2"/>
    <dbReference type="InParanoid" id="Q980R3"/>
    <dbReference type="PhylomeDB" id="Q980R3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00481">
        <property type="entry name" value="Ribosomal_eL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000231">
        <property type="entry name" value="Ribosomal_eL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="Ribosomal_eL30-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022991">
        <property type="entry name" value="Ribosomal_eL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002172">
        <property type="entry name" value="PRK01018.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00709">
        <property type="entry name" value="RIBOSOMAL_L30E_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00993">
        <property type="entry name" value="RIBOSOMAL_L30E_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000146159" description="Large ribosomal subunit protein eL30">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="108" mass="12095" checksum="4B24861EC334AA2C" modified="2001-10-01" version="1">MIKVSEQQVSFEGELKSLLKTGKVIFGAKRAIKYIKLSKVKMVIVASTLRGDLKQDIMHYAKLSNIPVYEYKGSGWDLGTLCGKPFMISTISIIDEGDSKILDIIKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q980R4</accession>
    <name>Q980R4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable transcription termination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">nusA</name>
        <name evidence="10" type="ordered locus">SSO0220</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Participates in transcription termination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NusA family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90163">
        <property type="entry name" value="D90163"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980R4"/>
    <dbReference type="STRING" id="273057.SSO0220"/>
    <dbReference type="PaxDb" id="273057-SSO0220"/>
    <dbReference type="EnsemblBacteria" id="AAK40563">
        <property type="protein sequence ID" value="AAK40563"/>
        <property type="gene ID" value="SSO0220"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0220"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.218"/>
    <dbReference type="eggNOG" id="arCOG01760">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131906_0_0_2"/>
    <dbReference type="InParanoid" id="Q980R4"/>
    <dbReference type="PhylomeDB" id="Q980R4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22531">
        <property type="entry name" value="KH-II_NusA_arch_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000041">
        <property type="entry name" value="Probable transcription termination protein NusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_A">
        <property type="entry name" value="NusA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058582">
        <property type="entry name" value="KH_NusA_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010212">
        <property type="entry name" value="NusA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="TF_NusA_bacterial"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01952">
        <property type="entry name" value="nusA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR NUSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648:SF0">
        <property type="entry name" value="TRANSCRIPTION TERMINATION_ANTITERMINATION PROTEIN NUSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26594">
        <property type="entry name" value="KH_NusA_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 6" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="KH type-2" evidence="8">
        <location>
            <begin position="2"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="NusA-like second KH" evidence="9">
        <location>
            <begin position="59"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF26594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK40563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="124" mass="13812" checksum="C14B2798826A5A4E" modified="2001-10-01" version="1">MTRANVRDCIIDNENNRIIFLVDSKDMGVAIGKSGINVKKLKKIIGKDIELVAYSDNLEELVKNLMSPARVRSVKVVNTSSKKSVYINIDPQDKGLAIGKNGRNVARAKLILKRYMDIDNVVIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-01-24" modified="2026-01-28" version="150" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UWW6</accession>
    <name>AATA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">A-type ATP synthase subunit A</fullName>
            <ecNumber evidence="1">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpA</name>
        <name type="ordered locus">SSO0563</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane. The A chain is the catalytic subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90202">
        <property type="entry name" value="H90202"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991074.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UWW6"/>
    <dbReference type="SMR" id="Q9UWW6"/>
    <dbReference type="FunCoup" id="Q9UWW6">
        <property type="interactions" value="233"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0563"/>
    <dbReference type="PaxDb" id="273057-SSO0563"/>
    <dbReference type="EnsemblBacteria" id="AAK40879">
        <property type="protein sequence ID" value="AAK40879"/>
        <property type="gene ID" value="SSO0563"/>
    </dbReference>
    <dbReference type="GeneID" id="1454846"/>
    <dbReference type="KEGG" id="sso:SSO0563"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.572"/>
    <dbReference type="eggNOG" id="arCOG00868">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008162_3_1_2"/>
    <dbReference type="InParanoid" id="Q9UWW6"/>
    <dbReference type="PhylomeDB" id="Q9UWW6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18119">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01134">
        <property type="entry name" value="V_A-ATPase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000008">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00309">
        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005726">
        <property type="entry name" value="ATP_synth_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01043">
        <property type="entry name" value="ATP_syn_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003220">
        <property type="entry name" value="PRK04192.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000144606" description="A-type ATP synthase subunit A">
        <location>
            <begin position="1"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="233"/>
            <end position="240"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00309"/>
        </source>
    </evidence>
    <sequence length="592" mass="66412" checksum="04875AD8DC7ADB07" modified="2000-05-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX81</accession>
    <name>KCY_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Cytidylate kinase</fullName>
            <shortName>CK</shortName>
            <ecNumber>2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Cytidine monophosphate kinase</fullName>
            <shortName>CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cmk</name>
        <name type="ordered locus">SSO0692</name>
        <name type="ORF">C10_038</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>CMP + ATP = CDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>dCMP + ATP = dCDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylate kinase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99217">
        <property type="entry name" value="A99217"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991241.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX81"/>
    <dbReference type="SMR" id="Q9UX81"/>
    <dbReference type="FunCoup" id="Q9UX81">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0692"/>
    <dbReference type="PaxDb" id="273057-SSO0692"/>
    <dbReference type="EnsemblBacteria" id="AAK40992">
        <property type="protein sequence ID" value="AAK40992"/>
        <property type="gene ID" value="SSO0692"/>
    </dbReference>
    <dbReference type="GeneID" id="1454959"/>
    <dbReference type="GeneID" id="44129690"/>
    <dbReference type="KEGG" id="sso:SSO0692"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.692"/>
    <dbReference type="eggNOG" id="arCOG01037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079959_1_0_2"/>
    <dbReference type="InParanoid" id="Q9UX81"/>
    <dbReference type="PhylomeDB" id="Q9UX81"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00239">
        <property type="entry name" value="Cytidyl_kinase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011892">
        <property type="entry name" value="Cyt_kin_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02173">
        <property type="entry name" value="cyt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13189">
        <property type="entry name" value="Cytidylate_kin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000132024" description="Cytidylate kinase">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="189" mass="21792" checksum="5E5C0FB3D71C140D" modified="2000-05-01" version="1">MIIIISGPPGSGKTSVAIKLANELSYKFISAGKIFRDIAQSMGLDIINLNRVAESNFDIDKMVDKKIHEYVLRERNLIVESHIAGWIFREYTDVAIYLWAPLKIRANRIAIRDKISYVEAVSQIIRREYMHYKRFNKFYGIDINDLSVFDLVINTSNIDINNIVKLILDYLSSVSHNSQPLKEKNINDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-04-27" modified="2024-05-29" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>P58029</accession>
    <name>CBSA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Cytochrome b558/566 subunit A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cbsA</name>
        <name type="ordered locus">SSO2801</name>
        <name type="ORF">C48_011</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Monoheme cytochrome whose physiological function is not yet clear.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cell membrane</location>
            <topology>Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90457">
        <property type="entry name" value="C90457"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58029"/>
    <dbReference type="SMR" id="P58029"/>
    <dbReference type="STRING" id="273057.SSO2801"/>
    <dbReference type="GlyCosmos" id="P58029">
        <property type="glycosylation" value="13 sites, No reported glycans"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2801"/>
    <dbReference type="EnsemblBacteria" id="AAK42914">
        <property type="protein sequence ID" value="AAK42914"/>
        <property type="gene ID" value="SSO2801"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2801"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2887"/>
    <dbReference type="eggNOG" id="arCOG06015">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_575729_0_0_2"/>
    <dbReference type="InParanoid" id="P58029"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09624">
        <property type="entry name" value="DOMON_b558_566"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019020">
        <property type="entry name" value="Cyt-c552/DMSO_Rdtase_haem-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017572">
        <property type="entry name" value="Cyt_b558/566_suA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03154">
        <property type="entry name" value="sulfolob_CbsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09459">
        <property type="entry name" value="EB_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00887">
        <property type="entry name" value="EB_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0349">Heme</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000089384" description="Cytochrome b558/566 subunit A">
        <location>
            <begin position="1"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="1">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="1">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <sequence length="479" mass="52532" checksum="7C92B0B91A8245B4" modified="2001-04-27" version="1">MLKPFCEKMSIKRKSKYTLGVLLLASFLAIIMGLANVPMAQTSPQIPVYKVVGNADLSNPGSASYWSQIPWINISLTANIPMAPTSGLTHYLLVKAVWNGSWIIILERWYAPEPAFGAWSAAAAALYPPASGPGLFRQIMLTPGTTYTIEKNYTNYFSIVNGNIIQGRLVLNYSGILLPAPNDTQITVLSNGTIILWHSPRPIEDLLYSDGMFYGYYTNNTWYYPDRAAIMWYMGSVIPPTKDGMNIGGKVPGQAFDGVTFNDTGGSLVQPGGAANIWMWVSGATWNNATYDPAFKVNLWQNTSLTGLPYIDPDNHGFAVPLYTNNTNMYEVDTAGIWYTPVTTSGLNGSLFFIWTGATYQNGYWTVEFARPLAVPSAYAKWMPNITVGKTYYVAFAVWQGKLGETLFDKSITSNFLTLELVTTPPTSTTTSTISSTSVTTTTSITTVTSIPSTTIYVTIVGVVIAIIALIILYVVFRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>P95931</accession>
    <name>PGP2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Phosphoglycolate phosphatase 2</fullName>
            <shortName>PGP 2</shortName>
            <shortName>PGPase 2</shortName>
            <ecNumber>3.1.3.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2157</name>
        <name type="ORF">C01033</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dephosphorylation of 2-phosphoglycolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>2-phosphoglycolate + H2O = glycolate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="EC" id="3.1.3.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal SPP-like hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.18"/>
    <dbReference type="EMBL" id="Y08256">
        <property type="protein sequence ID" value="CAA69435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74064">
        <property type="entry name" value="S74064"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992158.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95931"/>
    <dbReference type="SMR" id="P95931"/>
    <dbReference type="STRING" id="273057.SSO2157"/>
    <dbReference type="PaxDb" id="273057-SSO2157"/>
    <dbReference type="DNASU" id="1453660"/>
    <dbReference type="EnsemblBacteria" id="AAK42333">
        <property type="protein sequence ID" value="AAK42333"/>
        <property type="gene ID" value="SSO2157"/>
    </dbReference>
    <dbReference type="GeneID" id="1453660"/>
    <dbReference type="KEGG" id="sso:SSO2157"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2250"/>
    <dbReference type="eggNOG" id="arCOG01213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237940_0_0_2"/>
    <dbReference type="InParanoid" id="P95931"/>
    <dbReference type="PhylomeDB" id="P95931"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01419">
        <property type="entry name" value="GPH_hydrolase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006382">
        <property type="entry name" value="PGPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01487">
        <property type="entry name" value="Pglycolate_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01482">
        <property type="entry name" value="SPP-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000:SF8">
        <property type="entry name" value="HAD SUPERFAMILY HYDROLASE-LIKE, TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000146729" description="Phosphoglycolate phosphatase 2">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="233" mass="26215" checksum="5E3AE64515CAAD90" modified="1997-05-01" version="1">MDIGSNEILVASDYDRTLASEENNFVISPIVSQKVNEFSKKYKFVVVTGREKKFIDKLAVGLKPTAWILENGSLILFNDREFVLCEKSWFERDRKKIIEILDNLKVRYSIGRIILYVDGYGSKLDMLSEIEKYGRIEVNRNDAMILPKGVDKGVGVLKFKELTGFKGKIIALGDSENDYALFRVADIKVAVANAIPQIKEIADIVTENPNGLGVVEILDKILSGNFGKEVDIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-03-27" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX42</accession>
    <name>Q7LX42_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Clan AA aspartic protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2164</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74071">
        <property type="entry name" value="S74071"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX42"/>
    <dbReference type="STRING" id="273057.SSO2164"/>
    <dbReference type="PaxDb" id="273057-SSO2164"/>
    <dbReference type="EnsemblBacteria" id="AAK42340">
        <property type="protein sequence ID" value="AAK42340"/>
        <property type="gene ID" value="SSO2164"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2164"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2257"/>
    <dbReference type="eggNOG" id="arCOG04029">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161799_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX42"/>
    <dbReference type="PhylomeDB" id="Q7LX42"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022274">
        <property type="entry name" value="Peptidase_asp_AF0612"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03698">
        <property type="entry name" value="clan_AA_DTGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14161" checksum="FABDD916B3B11DC4" modified="2011-05-31" version="1">MGMVFDCFRINDKPTLPVTVIDPYNGDFMEVRALIDTGFSGYLLIPPSVYNKVNSLELENPRVYATLNGIVKTRVAKAIIKIGKIKLDTLIESPILGREISLLGRELQKILHIEFKKGKEVCIEDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX63</accession>
    <name>Q7LX63_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator PadR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S78039">
        <property type="entry name" value="S78039"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988333.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX63"/>
    <dbReference type="STRING" id="273057.SSO2138"/>
    <dbReference type="PaxDb" id="273057-SSO2138"/>
    <dbReference type="EnsemblBacteria" id="AAK42316">
        <property type="protein sequence ID" value="AAK42316"/>
        <property type="gene ID" value="SSO2138"/>
    </dbReference>
    <dbReference type="GeneID" id="1453643"/>
    <dbReference type="KEGG" id="sso:SSO2138"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2229"/>
    <dbReference type="eggNOG" id="arCOG00002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_1_3_2"/>
    <dbReference type="InParanoid" id="Q7LX63"/>
    <dbReference type="PhylomeDB" id="Q7LX63"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR YQJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, PADR-LIKE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="17"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="135" mass="15777" checksum="B464F50CDA34C4BF" modified="2011-05-31" version="1">MSELIARQKGRLRLMTLWLLWQSPRRGIEIIDDINKMTWGFWKPSPGSIYPLLNKMEEEGVIEKTSDGKYKITAKGSEEIKEFLPIKQINSIEDSVQELEGLAQFFKEVERNKLIEYKHRIISAIKEIEEVVKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXG5</accession>
    <name>Q7LXG5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="14">UDP-glucose 4-epimerase (GalE-2)</fullName>
            <ecNumber evidence="14">5.1.3.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">galE-2</name>
        <name evidence="14" type="ordered locus">SSO0809</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">Golgi stack membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="5.1.3.2" evidence="14"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90231">
        <property type="entry name" value="E90231"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXG5"/>
    <dbReference type="FunCoup" id="Q7LXG5">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0809"/>
    <dbReference type="PaxDb" id="273057-SSO0809"/>
    <dbReference type="EnsemblBacteria" id="AAK41108">
        <property type="protein sequence ID" value="AAK41108"/>
        <property type="gene ID" value="SSO0809"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0809"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.822"/>
    <dbReference type="eggNOG" id="arCOG01369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_4_0_2"/>
    <dbReference type="InParanoid" id="Q7LXG5"/>
    <dbReference type="PhylomeDB" id="Q7LXG5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048040">
        <property type="term" value="F:UDP-glucuronate decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05230">
        <property type="entry name" value="UGD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000065">
        <property type="entry name" value="UDP-glucuronic acid decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044516">
        <property type="entry name" value="UXS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078:SF6">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01713">
        <property type="entry name" value="NUCEPIMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="13">
        <location>
            <begin position="5"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAK41108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="310" mass="35279" checksum="32414BD692F5C628" modified="2011-05-31" version="1">MLMKILISGGAGFLGSHLTEALLEKGEEITIVDDLSTAKYFNIRKDVEFIKKKVEEFETEKKYDVVIHLAARPSPEDYIEHPVDTALSNSLGTYKMLEIARKSNARFIYTSSSEVYGSASIIPTPETYWGYVNPIGIRSCYDESKRFSEALIMAYHRQYKLDTRIQRPFNVYGPGLREDGTYGRVVSRFIYQALKGEDVTVFGDGNQTRAFLYISDWVDATIKLIYKDGLEGEVFNIGSDKEIKIIELANMIIKLTGSKSRIKYLPPRPDDPPRRAADITKAKEKLGWYPKISLEEGLKLTINWFKGVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-02-05" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXH1</accession>
    <name>Q7LXH1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial transposase in ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90230">
        <property type="entry name" value="E90230"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXH1"/>
    <dbReference type="STRING" id="273057.SSO0802"/>
    <dbReference type="PaxDb" id="273057-SSO0802"/>
    <dbReference type="EnsemblBacteria" id="AAK41100">
        <property type="protein sequence ID" value="AAK41100"/>
        <property type="gene ID" value="SSO0802"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0802"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.810"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146471_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXH1"/>
    <dbReference type="PhylomeDB" id="Q7LXH1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="1">
        <location>
            <begin position="94"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="149" mass="16989" checksum="2504FF0EB7080973" modified="2011-05-31" version="1">MLICEFTLSDARELTSQWDFIVKSIVRVKNRLRSDLILLGYSDSLSRKNLSEVLEGGDNIILSEVKFLLDELERLEARKREVEKRLEDVVPRDSLIFTIPGIGKTLGLIILGLGDVRRFDDKRKFVAYCGLDPVVEWGECCVARCLKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXH4</accession>
    <name>Q7LXH4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0799</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90230">
        <property type="entry name" value="B90230"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923092.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXH4"/>
    <dbReference type="STRING" id="273057.SSO0799"/>
    <dbReference type="PaxDb" id="273057-SSO0799"/>
    <dbReference type="EnsemblBacteria" id="AAK41097">
        <property type="protein sequence ID" value="AAK41097"/>
        <property type="gene ID" value="SSO0799"/>
    </dbReference>
    <dbReference type="GeneID" id="1455064"/>
    <dbReference type="KEGG" id="sso:SSO0799"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.805"/>
    <dbReference type="eggNOG" id="arCOG02218">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04033">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104053_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXH4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="183"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="232" mass="27066" checksum="0DCB14D7F7DBAE9F" modified="2011-05-31" version="1">MQVVEYSISTYLPRDVVYFDGTSVTLPHEYVIKEGNLRLFVPKNKINDVVNALKSAGFKGEKYSLSTKFFNIWELHVRIYDDGFIDAHFEVSRDYLEHLTYPTIPSIYEVFEIYRTVYDKLHIFDNGAKKWIKEVKTHYFVTLNPPRSITALQPITVSVGALSAIGILAYLLSRLDKGEDNIREVVEKALDEAIKEKEKERLRETALKIKELMKDVNEEEWATVIRENRNER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-03-27" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXJ9</accession>
    <name>Q7LXJ9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YbhB/YbcL family Raf kinase inhibitor-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0767</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99226">
        <property type="entry name" value="A99226"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXJ9"/>
    <dbReference type="STRING" id="273057.SSO0767"/>
    <dbReference type="MEROPS" id="I51.003"/>
    <dbReference type="PaxDb" id="273057-SSO0767"/>
    <dbReference type="EnsemblBacteria" id="AAK41064">
        <property type="protein sequence ID" value="AAK41064"/>
        <property type="gene ID" value="SSO0767"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0767"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.766"/>
    <dbReference type="eggNOG" id="arCOG04702">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083918_3_2_2"/>
    <dbReference type="InParanoid" id="Q7LXJ9"/>
    <dbReference type="PhylomeDB" id="Q7LXJ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00865">
        <property type="entry name" value="PEBP_bact_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005247">
        <property type="entry name" value="YbhB_YbcL/LppC-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00481">
        <property type="entry name" value="YbhB/YbcL family Raf kinase inhibitor-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30289:SF1">
        <property type="entry name" value="PEBP (PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30289">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YBCL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17903" checksum="6D66377C5D56B06E" modified="2011-05-31" version="1">MNSESIYSMRVVSSAFKNEDFIPIKYTCDGQDLSPELEWDLVTNAKSYAIIVEDPDAPGGTFIHWVIYNITTNRLPEGVPRLYKSQYGVQGVNDFGNIGYNGPCPPKTHPPHRYYFYVYAIDTILLEIKNINADKLKSLMEGHGIERGFVMGKYKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXQ6</accession>
    <name>Q7LXQ6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VIT family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90212">
        <property type="entry name" value="C90212"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991186.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXQ6"/>
    <dbReference type="STRING" id="273057.SSO0649"/>
    <dbReference type="PaxDb" id="273057-SSO0649"/>
    <dbReference type="EnsemblBacteria" id="AAK40954">
        <property type="protein sequence ID" value="AAK40954"/>
        <property type="gene ID" value="SSO0649"/>
    </dbReference>
    <dbReference type="GeneID" id="1454921"/>
    <dbReference type="KEGG" id="sso:SSO0649"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.652"/>
    <dbReference type="eggNOG" id="arCOG01091">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1425143_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXQ6"/>
    <dbReference type="PhylomeDB" id="Q7LXQ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="19591" checksum="CD403B64A77BCBAE" modified="2011-05-31" version="1">MANKLYEALKYVVKYELKSSIRRYIVLGAFDGILLTLSILLAGVISHIGLTSISLSIYSGIIATAISSTWNALVVELGEKKTELEKIEKQVLKSLKGTIYDYSARIAAALAAIAHGISPFLGLLVLYAYEYSHSTMLSLGVGISSLAVLGLLYGEGIKAKISTMVQLIVAGIITALITLLVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXR3</accession>
    <name>Q7LXR3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="8">ATase</shortName>
            <ecNumber evidence="3 8">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">purF-2</name>
        <name evidence="12" type="ordered locus">SSO0633</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 8">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="3 8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90210">
        <property type="entry name" value="G90210"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXR3"/>
    <dbReference type="FunCoup" id="Q7LXR3">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0633"/>
    <dbReference type="PaxDb" id="273057-SSO0633"/>
    <dbReference type="EnsemblBacteria" id="AAK40942">
        <property type="protein sequence ID" value="AAK40942"/>
        <property type="gene ID" value="SSO0633"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0633"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.638"/>
    <dbReference type="eggNOG" id="arCOG00094">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022389_3_1_2"/>
    <dbReference type="InParanoid" id="Q7LXR3"/>
    <dbReference type="PhylomeDB" id="Q7LXR3"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 8 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="5"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK40942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="404" mass="44839" checksum="DFFF4D11A07FD8A0" modified="2011-05-31" version="1">MILMAGILGILAFDDVWNVSRFLYYGLIGLQHRGYSKSGIAILNKQRNIFTKLENVAPEDLEIKDLNGWAGIGYAGIRNSYPITIDNGAIVVDGMIDDNNVLNHVIREPENAIDDIKKPISFIAISKDGQLIAYRDELGLKPLSIGGFGFDLAVISSEPTSMFVIGAEFKREINPGELVIVDKYHIESKQVRIPRKSYCTIEYVYQARIDSMINEREIYDLRVKIGEELALEYPINADSVIGVPETALPFAIGYSKKLNIPLDLGFTRTGSPIRTMLSSDAFLKIVGVQLKLNPIKSAVKGKRIILIDDSMVTGTTLKNTIFNLRKLGAKEIHVLIGSPKLVSQCPYGVEVPDEKELISANLNDETIARVLGADSIHWLSIEGLFKAIGHRDLCLGCMTKKYPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXR5</accession>
    <name>Q7LXR5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Methionyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">metS-like</name>
        <name evidence="17" type="ordered locus">SSO0627</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90210">
        <property type="entry name" value="A90210"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXR5"/>
    <dbReference type="FunCoup" id="Q7LXR5">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0627"/>
    <dbReference type="PaxDb" id="273057-SSO0627"/>
    <dbReference type="EnsemblBacteria" id="AAK40936">
        <property type="protein sequence ID" value="AAK40936"/>
        <property type="gene ID" value="SSO0627"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0627"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.632"/>
    <dbReference type="eggNOG" id="arCOG01136">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065946_3_1_2"/>
    <dbReference type="InParanoid" id="Q7LXR5"/>
    <dbReference type="PhylomeDB" id="Q7LXR5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02800">
        <property type="entry name" value="tRNA_bind_EcMetRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000042">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004495">
        <property type="entry name" value="Met-tRNA-synth_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00399">
        <property type="entry name" value="metG_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF37">
        <property type="entry name" value="TRNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="16">
        <location>
            <begin position="57"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AAK40936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="162" mass="18220" checksum="1349858C854F2AC1" modified="2011-05-31" version="1">MVFPSEILLTSITTILHNRYKKLASNKYKLLLHYVNQNPYNSSPMLNIMSNQITIDDFAKLDLKVGIVRQAERIEGTRLLKLLIDLGSEQRQIIAGLGEYYTPEELLNKRIIVIANLKPRVIRGFESQGMLLAAGCKEDEAKGIKPRILTVDGEVPPGTKIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXR9</accession>
    <name>Q7LXR9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0619</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="C90209"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991153.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXR9"/>
    <dbReference type="STRING" id="273057.SSO0619"/>
    <dbReference type="PaxDb" id="273057-SSO0619"/>
    <dbReference type="EnsemblBacteria" id="AAK40930">
        <property type="protein sequence ID" value="AAK40930"/>
        <property type="gene ID" value="SSO0619"/>
    </dbReference>
    <dbReference type="GeneID" id="1454897"/>
    <dbReference type="KEGG" id="sso:SSO0619"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.627"/>
    <dbReference type="eggNOG" id="arCOG00454">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137725_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXR9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053658">
        <property type="entry name" value="Cdv_Coordination_Protein"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041009">
        <property type="entry name" value="cell_div_CdvB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="168" mass="19290" checksum="6968D2D795D353E4" modified="2011-05-31" version="1">MKKKGKSLAELLIDVRIARNKVQNIINRMQNKIGTYNHIFMRNVASFPHLSKMVARESELLENVMDHLLTLEVILEILEIKIETIIYIGNIVTSAASVVEAIKLLKESFNLTPDISLLLDDIYSNFYVNVDLPKEIKINVKEEARSVLLDAEKIAEKRKSETYYQVNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXS8</accession>
    <name>Q7LXS8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90207">
        <property type="entry name" value="D90207"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991127.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXS8"/>
    <dbReference type="STRING" id="273057.SSO0604"/>
    <dbReference type="PaxDb" id="273057-SSO0604"/>
    <dbReference type="EnsemblBacteria" id="AAK40915">
        <property type="protein sequence ID" value="AAK40915"/>
        <property type="gene ID" value="SSO0604"/>
    </dbReference>
    <dbReference type="GeneID" id="1454882"/>
    <dbReference type="KEGG" id="sso:SSO0604"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.611"/>
    <dbReference type="eggNOG" id="arCOG01357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032214_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXS8"/>
    <dbReference type="PhylomeDB" id="Q7LXS8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731:SF4">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731">
        <property type="entry name" value="SLL1084 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="217"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="506" mass="58355" checksum="9ECE2B38D7901752" modified="2011-05-31" version="1">MSFLEQTFDFILTTDRCLMTNHHGKEFLGFLGTGPAVGVPEAVWKWLACPKMKVDDLGRPEQAPYGMRKVEAKLIDEGFKAAIIDPDHLDRHLKYAKALMFSHHDYFAFGPPSSTWWGITKKEPINYKSFQALISKPEIQEAKKRGMKILVGGPSTWQWLWREDMIEKVGVDTLVDGEGEKVVVKLAQMILDNEPLPKYVYVSGDDVPDIDDISEIKGASVNGMIEIMRGCARSCRFCSVTIRPTRYYPLEKIEKELQTNVRAGVRHGVVHSDDVLFYGAVGIYPRPEPLIKLHTLVKKYYKTIAWSHASLAAIRYSEEKYGLISKLGEIIFDDEQRYLGVEVGIETGSVKLAKEIMPAKSAPYKPEEYPEIVEEAFKIMHENKIIPAGTMIVGLPEETEDDVYRTIELVDNLRPYRSILVPMFFVPMGFFKNRDWFTRVKLSEAHIELYRKVFWHDVYWAEDIINSFYMKGPVYMPVRFALKLFLRFAKKKMREVEKWLENQLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXT9</accession>
    <name>Q7LXT9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0577</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90204">
        <property type="entry name" value="D90204"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991093.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXT9"/>
    <dbReference type="STRING" id="273057.SSO0577"/>
    <dbReference type="PaxDb" id="273057-SSO0577"/>
    <dbReference type="EnsemblBacteria" id="AAK40891">
        <property type="protein sequence ID" value="AAK40891"/>
        <property type="gene ID" value="SSO0577"/>
    </dbReference>
    <dbReference type="GeneID" id="1454858"/>
    <dbReference type="KEGG" id="sso:SSO0577"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.585"/>
    <dbReference type="eggNOG" id="arCOG05991">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2177744_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXT9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="130" mass="15246" checksum="24FDCD67BBADAECD" modified="2011-05-31" version="1">MTQERVVRILAYYRDPGLIERIASNFRKLFMDIEWIYGWKVNDENLYEFYVGVKDHNNFNTAVLLLSKTVDVSKVEILDDAQLKRVIIRDGKVIENQSEGVKEGDIIIYVPVFNKVKGYSWGEVYVKDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXV5</accession>
    <name>Q7LXV5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">7-cyano-7-deazaguanine synthase</fullName>
            <ecNumber evidence="9">6.3.4.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">SSO0544</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>7-carboxy-7-carbaguanine + NH4(+) + 2 ATP = 7-cyano-7-carbaguanine + 2 AMP + 2 diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:27982"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the QueC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.20" evidence="9"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90200">
        <property type="entry name" value="G90200"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992829.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXV5"/>
    <dbReference type="FunCoup" id="Q7LXV5">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0544"/>
    <dbReference type="PaxDb" id="273057-SSO0544"/>
    <dbReference type="EnsemblBacteria" id="AAK40862">
        <property type="protein sequence ID" value="AAK40862"/>
        <property type="gene ID" value="SSO0544"/>
    </dbReference>
    <dbReference type="GeneID" id="1454829"/>
    <dbReference type="KEGG" id="sso:SSO0544"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.548"/>
    <dbReference type="eggNOG" id="arCOG00039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081854_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LXV5"/>
    <dbReference type="PhylomeDB" id="Q7LXV5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01995">
        <property type="entry name" value="QueC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914">
        <property type="entry name" value="7-CYANO-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914:SF1">
        <property type="entry name" value="7-CYANO-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK40862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="245" mass="28450" checksum="FD68B57B6D2DD196" modified="2011-05-31" version="1">MVEEAIIQLTGGIDSTVLAYYLKKDKYVLHGTFIHYGYPPQIKELELVKELSKKLDIPLKIIDFSSYIKSFDLPPIDSIQYIIKYQFVVEILASFSAYPNLNTMFVGWLKDEWNNRISLLKDIESLMGFKVIAPFHTMVKSEVIKLGNELGVDFTKTHSCIVSGKMHCGICMACRSRRRAFEEVKVKDPTIYYYNLPPAEVDKLSRELSLEEFVERFFKPFLQYTGEYPKEFVDNLAHILRKYNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXX7</accession>
    <name>Q7LXX7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type II secretion system protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75370">
        <property type="entry name" value="S75370"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXX7"/>
    <dbReference type="STRING" id="273057.SSO0119"/>
    <dbReference type="PaxDb" id="273057-SSO0119"/>
    <dbReference type="EnsemblBacteria" id="AAK40474">
        <property type="protein sequence ID" value="AAK40474"/>
        <property type="gene ID" value="SSO0119"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0119"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.114"/>
    <dbReference type="eggNOG" id="arCOG01815">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_558551_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXX7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056569">
        <property type="entry name" value="ArlJ-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF046075">
        <property type="entry name" value="UpsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402:SF1">
        <property type="entry name" value="TYPE II SECRETION SYSTEM PROTEIN GSPF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="484"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="506" mass="58973" checksum="8E36B837A0F08765" modified="2011-05-31" version="1">MIKLKLKLFKKMNLFELMNTRINENIKYYGDDMERLRKEYIVMLFLMPLISSISIILYLKISPYFLLLNIMNVFIYFFPILITQIRKDEQRKLIENEMPVFLLFAYVNSLLGRNLYKTFEEIRNSKVFKGLRREAMLLVKEVEVLGKSSFSAMESRAKAHRGDFLGKIYTVYTSGESIGISMPERLKDLLNETIDGLNSNFQGYVEKVNELVEILFMLFLITPMILLAFQYISSSINIFELIFPLLLFPIIFFYISLIQPNIGYDIKIDIKEVKNSLYILPIPFILVFLFHLSLEYEILVFYSIFIMFSFFIYRKISVADAILNNLPYILSDIADYLKIGYSIKSAILKLNVDNTHFRMFLGELAAKIRKNEAISNVRTNIWIVNAILELIENIDKKGFADTYTFKDLSLILYNYTSLRKKVLQNLRLFSILATITPIVFYFALGIMSKIRAVGNLDLIIVLYTMALSIIYAKVSRFTVFNFPLLVLALMNLIIVLLFGNVILTFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LY04</accession>
    <name>Q7LY04_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1614 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75432">
        <property type="entry name" value="S75432"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988857.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LY04"/>
    <dbReference type="STRING" id="273057.SSO0055"/>
    <dbReference type="PaxDb" id="273057-SSO0055"/>
    <dbReference type="DNASU" id="1455311"/>
    <dbReference type="EnsemblBacteria" id="AAK40420">
        <property type="protein sequence ID" value="AAK40420"/>
        <property type="gene ID" value="SSO0055"/>
    </dbReference>
    <dbReference type="GeneID" id="1455311"/>
    <dbReference type="KEGG" id="sso:SSO0055"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.59"/>
    <dbReference type="eggNOG" id="arCOG02159">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082100_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LY04"/>
    <dbReference type="PhylomeDB" id="Q7LY04"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011672">
        <property type="entry name" value="DUF1614"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07758">
        <property type="entry name" value="DUF1614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="243" mass="26924" checksum="E6251F3987F6C63D" modified="2011-05-31" version="1">MKRIIILSPFRGLFRSLLYFLLGLIMALISAGYFSQLFSIVGINRDIAIIISITISFLSLFSSPFNLVLTEVKKEAIAIEEDVVFFFGFPIFIPRISRQNMNTLIAVNLGGALIPLGVSLFLMYELQAYIVYFIINIIVVIIVSKLFSRVIRGVGVIMHPIIPSIFSVISSYVLFYKLHILIPLSAYIGSVLGTLIGADLLNLRRIINEARPQVISIGGMGTFDGIFVSGIISVFISELLVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LY05</accession>
    <name>Q7LY05_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MobA-like NTP transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0054</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75434">
        <property type="entry name" value="S75434"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LY05"/>
    <dbReference type="SMR" id="Q7LY05"/>
    <dbReference type="FunCoup" id="Q7LY05">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0054"/>
    <dbReference type="PaxDb" id="273057-SSO0054"/>
    <dbReference type="EnsemblBacteria" id="AAK40419">
        <property type="protein sequence ID" value="AAK40419"/>
        <property type="gene ID" value="SSO0054"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0054"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.57"/>
    <dbReference type="eggNOG" id="arCOG01871">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098907_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LY05"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136:SF86">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-PHOSPHATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MobA-like NTP transferase" evidence="2">
        <location>
            <begin position="18"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="179" mass="20525" checksum="F9B1C701178DAFB2" modified="2011-05-31" version="1">MLEHLIKISRRNWSSLKVIILAGGRGKRITLFKPFLVVCGKPLISWAFDAVNKLSDKVYLGINQSHPLFTIFKHSIFKIFPTPDISYENDVKYVVESLGPPILVLPVDIAFINNNIINNLIERCAVDMCTLKSYGSYLGVTYWTGLNFSNYTDIEVKEKLYNINTWEDYIKANKECNIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-26" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TY2</accession>
    <name>Y3251_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0282 protein SSO3251</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO3251</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0282 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90510">
        <property type="entry name" value="G90510"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989655.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TY2"/>
    <dbReference type="STRING" id="273057.SSO3251"/>
    <dbReference type="PaxDb" id="273057-SSO3251"/>
    <dbReference type="EnsemblBacteria" id="AAK43344">
        <property type="protein sequence ID" value="AAK43344"/>
        <property type="gene ID" value="SSO3251"/>
    </dbReference>
    <dbReference type="GeneID" id="1453261"/>
    <dbReference type="KEGG" id="sso:SSO3251"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3349"/>
    <dbReference type="eggNOG" id="arCOG00969">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079268_0_0_2"/>
    <dbReference type="InParanoid" id="Q97TY2"/>
    <dbReference type="PhylomeDB" id="Q97TY2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01406">
        <property type="entry name" value="UPF0282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050114">
        <property type="entry name" value="UPF0173_UPF0282_UlaG_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014426">
        <property type="entry name" value="UPF0282_hydrls"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003287">
        <property type="entry name" value="PRK04286.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546">
        <property type="entry name" value="UPF0173 METAL-DEPENDENT HYDROLASE MJ1163-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546:SF4">
        <property type="entry name" value="UPF0282 PROTEIN MJ1629"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004944">
        <property type="entry name" value="UCP004944_hydrls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000057640" description="UPF0282 protein SSO3251">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01406"/>
        </source>
    </evidence>
    <sequence length="308" mass="34895" checksum="9112788BD5164F99" modified="2001-10-01" version="1">MKITPIAFESLGVRSQATLIETKDLRVLVDPAVSLAPRRYNLPPHQREVDRLTQLAKVLVDVAKDVDVIVVSHYHYDHHDPGYVIPTDIYKNKIVFLKDPQNMINNSQKYRRAPRFLRSIKDKPRKIEIADGKTFEFNTTTISFSPAVPHGADERLGYVIQVAISDKDSTVIFTSDIEGAPKDVHLKFTKEKMPTVIIIDGPLSYLLGRALKEEELENSIRNMEEIIKNGLETVIIDHHVLRDINYAQVLKPVHDIAKDLGVRVTTAAEYLNLEPLILEARRKELYKEDNRPAKIPRGLANLLNAGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TY3</accession>
    <name>Q97TY3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO3250</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90510">
        <property type="entry name" value="F90510"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989657.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TY3"/>
    <dbReference type="STRING" id="273057.SSO3250"/>
    <dbReference type="PaxDb" id="273057-SSO3250"/>
    <dbReference type="EnsemblBacteria" id="AAK43343">
        <property type="protein sequence ID" value="AAK43343"/>
        <property type="gene ID" value="SSO3250"/>
    </dbReference>
    <dbReference type="GeneID" id="1453260"/>
    <dbReference type="KEGG" id="sso:SSO3250"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3348"/>
    <dbReference type="eggNOG" id="arCOG01096">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038957_2_1_2"/>
    <dbReference type="InParanoid" id="Q97TY3"/>
    <dbReference type="PhylomeDB" id="Q97TY3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="247" mass="27514" checksum="8E4DE7E13DF53836" modified="2001-10-01" version="1">MEKEKGEELVHYIHEADIFRTKVFGIQDGLIGVGAIALGAAGFSHDAIAVLVAGLIATIGQAFSMGIGEYISTRVRMQVIQNEIRKEKYQLKKFPEMEKQELVNFYMKKGFTKEVSEKIADYLLKNEDVALEEMLMHELKVFPEEFERPVKLGFLMSFYLIFGGLIPVFPFAISTYLKQFGFNFALITSILLVILTLGIFGILGTKYTGLSKPRGALEQIGTGLIALMGSYLAGVILAHFIPISYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TZ2</accession>
    <name>Q97TZ2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbon monoxide dehydrogenase, large chain (CutA-8)</fullName>
            <ecNumber evidence="4">1.2.7.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cutA-8</name>
        <name evidence="4" type="ordered locus">SSO3239</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.4" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90509">
        <property type="entry name" value="E90509"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989671.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TZ2"/>
    <dbReference type="FunCoup" id="Q97TZ2">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3239"/>
    <dbReference type="PaxDb" id="273057-SSO3239"/>
    <dbReference type="EnsemblBacteria" id="AAK43332">
        <property type="protein sequence ID" value="AAK43332"/>
        <property type="gene ID" value="SSO3239"/>
    </dbReference>
    <dbReference type="GeneID" id="1453251"/>
    <dbReference type="KEGG" id="sso:SSO3239"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3339"/>
    <dbReference type="eggNOG" id="arCOG01167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_2_0_2"/>
    <dbReference type="InParanoid" id="Q97TZ2"/>
    <dbReference type="PhylomeDB" id="Q97TZ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:anaerobic carbon-monoxide dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.50">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.365.10">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046867">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053554">
        <property type="entry name" value="Glyceraldehyde_dh-related"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041018">
        <property type="entry name" value="glyceraldDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908:SF132">
        <property type="entry name" value="ALDEHYDE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="MoCoBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20256">
        <property type="entry name" value="MoCoBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde oxidase/xanthine dehydrogenase a/b hammerhead" evidence="3">
        <location>
            <begin position="17"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="710" mass="79024" checksum="C5FCAF974DCE11C5" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U11</accession>
    <name>Q97U11_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NTP pyrophosphohydrolase MazG-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO12199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2004-09" db="PDB data bank">
            <title>Crystal structure of MazG nucleotide pyrophosphohydrolase (13816655) from Sulfolobus solfataricus at 1.46 A resolution.</title>
            <authorList>
                <consortium name="Joint Center for Structural Genomics (JCSG)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.46 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90506">
        <property type="entry name" value="H90506"/>
    </dbReference>
    <dbReference type="PDB" id="1VMG">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.46 A"/>
        <property type="chains" value="A=1-83"/>
    </dbReference>
    <dbReference type="PDBsum" id="1VMG"/>
    <dbReference type="AlphaFoldDB" id="Q97U11"/>
    <dbReference type="SMR" id="Q97U11"/>
    <dbReference type="STRING" id="273057.SSO12199"/>
    <dbReference type="PaxDb" id="273057-SSO12199"/>
    <dbReference type="EnsemblBacteria" id="AAK43311">
        <property type="protein sequence ID" value="AAK43311"/>
        <property type="gene ID" value="SSO12199"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO12199"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3317"/>
    <dbReference type="eggNOG" id="arCOG01084">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166059_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U11"/>
    <dbReference type="PhylomeDB" id="Q97U11"/>
    <dbReference type="EvolutionaryTrace" id="Q97U11"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd11535">
        <property type="entry name" value="NTP-PPase_SsMazG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1080">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004518">
        <property type="entry name" value="MazG-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42702">
        <property type="entry name" value="NUCLEOTIDE PYROPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42702:SF1">
        <property type="entry name" value="REGULATORY PROTEIN FOR BETA-LACTAMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03819">
        <property type="entry name" value="MazG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101386">
        <property type="entry name" value="all-alpha NTP pyrophosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP pyrophosphohydrolase MazG-like" evidence="1">
        <location>
            <begin position="28"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="1VMG"/>
        </source>
    </evidence>
    <sequence length="83" mass="9571" checksum="234170B691B7CD82" modified="2001-10-01" version="1">MDLELKELQSKMKEMYFEKDSQRGIYATFTWLVEEVGELAEALLSNNLDSIQEELADVIAWTVSIANLEGIDIEEALKKKYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UB1</accession>
    <name>Q97UB1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Twin-arginine translocase TatA/TatE family subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">SSO11972</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90494">
        <property type="entry name" value="C90494"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990928.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UB1"/>
    <dbReference type="STRING" id="273057.SSO11972"/>
    <dbReference type="PaxDb" id="273057-SSO11972"/>
    <dbReference type="EnsemblBacteria" id="AAK43210">
        <property type="protein sequence ID" value="AAK43210"/>
        <property type="gene ID" value="SSO11972"/>
    </dbReference>
    <dbReference type="GeneID" id="7798408"/>
    <dbReference type="KEGG" id="sso:SSO11972"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3214"/>
    <dbReference type="eggNOG" id="arCOG02694">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086034_3_1_2"/>
    <dbReference type="InParanoid" id="Q97UB1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.3310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006312">
        <property type="entry name" value="TatA/E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01411">
        <property type="entry name" value="tatAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982:SF1">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK43210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="10318" checksum="5895DF09A333A2BF" modified="2001-10-01" version="1">MIDNPTDIIIILVVIAVLFFGSSKMPELFRALGRSMGEFKKGQLEAEMEMQQMMSSNVVEKGERSESVEELEKKIVELQKQLEALKQSKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UD5</accession>
    <name>Q97UD5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90491">
        <property type="entry name" value="C90491"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989100.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UD5"/>
    <dbReference type="STRING" id="273057.SSO3082"/>
    <dbReference type="PaxDb" id="273057-SSO3082"/>
    <dbReference type="EnsemblBacteria" id="AAK43186">
        <property type="protein sequence ID" value="AAK43186"/>
        <property type="gene ID" value="SSO3082"/>
    </dbReference>
    <dbReference type="GeneID" id="7808556"/>
    <dbReference type="KEGG" id="sso:SSO3082"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3188"/>
    <dbReference type="eggNOG" id="arCOG07731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1478828_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UD5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="205" mass="23369" checksum="202584EEA5ED72F2" modified="2001-10-01" version="1">MGVVSFLIKHRYGEYGLLSYIDLELALSVSIVASFNFFHPQTRNASVDQLLFNIITFTIGIILSTLVIRNICLGMGQDLYDGTIISFLQMRNRRAIFLLSYFIDVLLIGGLFLLIAELVFLLSSFTPPITWISIFISEYLLLCNSSYLIAILIKRPFRSFFISISLIFLVLGIEITGNMIIFNYLLPLDLILGHLAYYAFRKVQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UG5</accession>
    <name>Q97UG5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Maltose ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO3055</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90487">
        <property type="entry name" value="D90487"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UG5"/>
    <dbReference type="FunCoup" id="Q97UG5">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3055"/>
    <dbReference type="TCDB" id="3.A.1.5.8">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3055"/>
    <dbReference type="EnsemblBacteria" id="AAK43155">
        <property type="protein sequence ID" value="AAK43155"/>
        <property type="gene ID" value="SSO3055"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3055"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3153"/>
    <dbReference type="eggNOG" id="arCOG00185">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_86_2_2"/>
    <dbReference type="InParanoid" id="Q97UG5"/>
    <dbReference type="PhylomeDB" id="Q97UG5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000016">
        <property type="entry name" value="Oligopeptide ABC transporter ATP-binding component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008453">
        <property type="entry name" value="PRK11308.1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067:SF3">
        <property type="entry name" value="MALTOSE ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067">
        <property type="entry name" value="OLIGOPEPTIDE/DIPEPTIDE ABC TRANSPORTER, ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="9"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="357"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="617" mass="69433" checksum="00803C0F73246469" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UI0</accession>
    <name>Q97UI0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90485">
        <property type="entry name" value="D90485"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924103.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UI0"/>
    <dbReference type="STRING" id="273057.SSO3037"/>
    <dbReference type="PaxDb" id="273057-SSO3037"/>
    <dbReference type="EnsemblBacteria" id="AAK43139">
        <property type="protein sequence ID" value="AAK43139"/>
        <property type="gene ID" value="SSO3037"/>
    </dbReference>
    <dbReference type="GeneID" id="34442795"/>
    <dbReference type="KEGG" id="sso:SSO3037"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3136"/>
    <dbReference type="eggNOG" id="arCOG04132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1656967_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UI0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016675">
        <property type="entry name" value="UCP016666"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09910">
        <property type="entry name" value="DUF2139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="159" mass="18457" checksum="B51446734C14F15B" modified="2001-10-01" version="1">MDKPKLVLKEFVQHNEVALGHHQFRSLYIGFHPISRNMIVHDGHAAKVYSNGEVIYRYEKLGRAPRVAGDTHAALTWSKDLLFIGGWLKAPPGMIEVSQYERILKYQDMRMKYSHIHMIYDNDKVEVLWSRKWDDKIPPNGSVLMQSSYSKSISKIKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UJ5</accession>
    <name>Q97UJ5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO3016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90482">
        <property type="entry name" value="G90482"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UJ5"/>
    <dbReference type="PaxDb" id="273057-SSO3016"/>
    <dbReference type="EnsemblBacteria" id="AAK43118">
        <property type="protein sequence ID" value="AAK43118"/>
        <property type="gene ID" value="SSO3016"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3016"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3109"/>
    <dbReference type="eggNOG" id="arCOG01933">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044590_4_0_2"/>
    <dbReference type="InParanoid" id="Q97UJ5"/>
    <dbReference type="PhylomeDB" id="Q97UJ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01292">
        <property type="entry name" value="metallo-dependent_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000089">
        <property type="entry name" value="Amidohydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="2-AMINO-3-CARBOXYLMUCONATE-6-SEMIALDEHYDE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240:SF19">
        <property type="entry name" value="CATALYTIC_ HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="2">
        <location>
            <begin position="10"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="286" mass="33012" checksum="B970C4F0D82656D0" modified="2001-10-01" version="1">MKSYYMGYVDAHTHVWFKETLPKDFFSNDSGYEYTPPSVQEIIKEMDSVNIDYIVIIAYPSREIWRTKEDFPIAMIKFLKEYGNRFSIVGGIEPNRISLNEAKDWLERQYEAGVSGFKLHPVHSHVKPNAYREEEGGLKQLELLYEFAQDHNLPVVIHTGTSVFLKARNKYADPIFVDDVAVDFPQLKIVLAHMGRPNYVPTAFQLVRIRRNLYGEISSIPPKKLLEYLPRLEEISYKTIYGSDYGGPGIKSISYNLREFLSLNISEKAKLDMGSNNPKALYKPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UL9</accession>
    <name>Q97UL9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nickel/cobalt efflux system</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2984</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90479">
        <property type="entry name" value="B90479"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992584.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UL9"/>
    <dbReference type="PaxDb" id="273057-SSO2984"/>
    <dbReference type="DNASU" id="1453008"/>
    <dbReference type="EnsemblBacteria" id="AAK43089">
        <property type="protein sequence ID" value="AAK43089"/>
        <property type="gene ID" value="SSO2984"/>
    </dbReference>
    <dbReference type="GeneID" id="1453008"/>
    <dbReference type="KEGG" id="sso:SSO2984"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3076"/>
    <dbReference type="eggNOG" id="arCOG06012">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115694_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UL9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="18729" checksum="C91AEF8B8270CC4D" modified="2001-10-01" version="1">MDPFILSLLLGLSHGIEPDHVATARLLKSRWKIVQFALSHSAGFVIIAIPLVILIGDNKFLEIISNIIGIIFSILLLIQAIFDKEIDIGANKAGLLQGAFVITPTKVLVIVIASTAYSILYSIEVISVFIIASAVSIISLSLLNFVPKRVYKIVDVGIALLTMTYLIFLLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UN7</accession>
    <name>Q97UN7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2966</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90476">
        <property type="entry name" value="H90476"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924077.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UN7"/>
    <dbReference type="STRING" id="273057.SSO2966"/>
    <dbReference type="PaxDb" id="273057-SSO2966"/>
    <dbReference type="EnsemblBacteria" id="AAK43071">
        <property type="protein sequence ID" value="AAK43071"/>
        <property type="gene ID" value="SSO2966"/>
    </dbReference>
    <dbReference type="GeneID" id="1452990"/>
    <dbReference type="KEGG" id="sso:SSO2966"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3057"/>
    <dbReference type="eggNOG" id="arCOG03857">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1149849_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UN7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="252" mass="28209" checksum="C78935129438911B" modified="2001-10-01" version="1">MKLYFLSTIPIVMSLALAYMFPLNELIPIVTFWIFLTYLLFNTVFRAELQVMGSYKRKGTKWGIFFSYLITHYLVYSVAIVELLTYIYKPTISNPQTAFVSLFNTPFGISPSITNLGLSLIFNPTITAFIPPNIVIDLSLYSISMGLYIAILVTSSLITIISLNKKLKYLVFIPLIGIIAGASCCVSIPVLLAESVEVSNLIFLTTVGWQAVFIAYVSLPILTVIFLKHLSSSLHRLHSGLVEFNTLRKVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97US4</accession>
    <name>Q97US4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transposase IS4-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90471">
        <property type="entry name" value="E90471"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97US4"/>
    <dbReference type="STRING" id="273057.SSO2919"/>
    <dbReference type="PaxDb" id="273057-SSO2919"/>
    <dbReference type="EnsemblBacteria" id="AAK43028">
        <property type="protein sequence ID" value="AAK43028"/>
        <property type="gene ID" value="SSO2919"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2919"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3009"/>
    <dbReference type="eggNOG" id="arCOG02754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1551894_0_0_2"/>
    <dbReference type="InParanoid" id="Q97US4"/>
    <dbReference type="PhylomeDB" id="Q97US4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053172">
        <property type="entry name" value="Tn903_transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631:SF3">
        <property type="entry name" value="ISSOD12 TRANSPOSASE TNPA_ISSOD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="9"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="166" mass="19221" checksum="5C8FF0B7CDD3957A" modified="2001-10-01" version="1">MDNVTGGQYIVAKWGKTRDSKFLKIEIVMDNNEFNVVNAEVTSNEVESVKTVKDLQDKGKKVKKFYGDKAYDANEVYKTGVEVVVPPKKNASTKRHLARRKAVREFRKLGYDRWREEKGYGVRWRVESLFSAVKRTFGESVRQVLLELRLSSSSGLTRGWLTWRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UT2</accession>
    <name>Q97UT2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">sulfate adenylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP-sulfurylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Sulfate adenylate transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">sat</name>
        <name evidence="12" type="ordered locus">SSO2912</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90470">
        <property type="entry name" value="E90470"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993310.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UT2"/>
    <dbReference type="FunCoup" id="Q97UT2">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2912"/>
    <dbReference type="PaxDb" id="273057-SSO2912"/>
    <dbReference type="EnsemblBacteria" id="AAK43020">
        <property type="protein sequence ID" value="AAK43020"/>
        <property type="gene ID" value="SSO2912"/>
    </dbReference>
    <dbReference type="GeneID" id="1452939"/>
    <dbReference type="GeneID" id="44128639"/>
    <dbReference type="KEGG" id="sso:SSO2912"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3001"/>
    <dbReference type="eggNOG" id="arCOG04191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022950_1_1_2"/>
    <dbReference type="InParanoid" id="Q97UT2"/>
    <dbReference type="PhylomeDB" id="Q97UT2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00517">
        <property type="entry name" value="ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.10">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025980">
        <property type="entry name" value="ATP-Sase_PUA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024951">
        <property type="entry name" value="Sulfurylase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002650">
        <property type="entry name" value="Sulphate_adenylyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003166">
        <property type="entry name" value="PRK04149.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00339">
        <property type="entry name" value="sopT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43509">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43509:SF1">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01747">
        <property type="entry name" value="ATP-sulfurylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14306">
        <property type="entry name" value="PUA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-sulfurylase PUA-like" evidence="11">
        <location>
            <begin position="17"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Sulphate adenylyltransferase catalytic" evidence="10">
        <location>
            <begin position="161"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK43020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="406" mass="46996" checksum="8DF82324506FFBA2" modified="2001-10-01" version="1">MNLIGHGKVEIVERIKTISDFKELHRIEVKRQLAHEIVSIAYGFLSPLKGFMNYEEVDGVVENMRLPNGVLWPIPLVFDYSQNEKVKEGDTIGITYLGKPLAIMKVKEIFKYDKLKIAEKVYKTKDIKHPGVKRTLSYADAFLAGDVWLVREPQFNKPYSEFWLTPRMHRTVFEKKGWKRVVAFQTRNVPHTGHEYLMKFAWFAANENQKVDEPRTGILVNVVIGEKRVGDYIDEAILLTHDALSKYGYISPKVHLLSFTLWDMRYAGPREALLHAIIRSNLGCTHHVFGRDHAGVGNYYSPYEAHEIFDSINEEDLLIKPIFLRENYYCPRCGSIENEILCDHKDEKQEFSGSLIRSIILDEVKPTKMVMRPEVYDVLMKAAEQYGFGSPFVTEEYLEKRQSILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UU3</accession>
    <name>Q97UU3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99469">
        <property type="entry name" value="B99469"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993054.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UU3"/>
    <dbReference type="STRING" id="273057.SSO2900"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2900"/>
    <dbReference type="EnsemblBacteria" id="AAK43009">
        <property type="protein sequence ID" value="AAK43009"/>
        <property type="gene ID" value="SSO2900"/>
    </dbReference>
    <dbReference type="GeneID" id="1452928"/>
    <dbReference type="KEGG" id="sso:SSO2900"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2990"/>
    <dbReference type="eggNOG" id="arCOG01417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053640_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UU3"/>
    <dbReference type="PhylomeDB" id="Q97UU3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="208"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="378" mass="43331" checksum="2F858ECD8E60816B" modified="2001-10-01" version="1">MDILAVVDFGLNEKTGGYKRNFEIMKRLPKRVNIDIVPSIRNVRLYSFRKDLISLLKGLNSTPDYVIELLEKGSNVEEFLNGLKKKKYDVAVVYSNSSENVELARRITSAPIGVQLQLEPFYRDLTTLFKIKFRGVTGKAVRKFKEAMEESKKEELKWKGLIERGNLNFAISVSKVPLINSGLDRLLSYRVTKPGNAFDENMLKFRGDKEDYAVYFTRLMPEKGLFEIPLIWKKLRRDIRLYVMGQFVDESDKDDFLSLVKRLNVNVEYIGLKEEEELYSIVAKALFTVYPSHYDSFSLVVLESLALGTPVYAYDTLALREIFGNVKGVHLVREGDVKGLADLISSFKGEEVPIPEDYSSWDRVAEAELEDIKAFAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="142" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V01</accession>
    <name>Q97V01_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable branched-chain amino acid transport ATP-binding protein LivG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2836</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Probable component of a branched-chain amino-acid transport system.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90461">
        <property type="entry name" value="C90461"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923990.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V01"/>
    <dbReference type="FunCoup" id="Q97V01">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2836"/>
    <dbReference type="PaxDb" id="273057-SSO2836"/>
    <dbReference type="EnsemblBacteria" id="AAK42946">
        <property type="protein sequence ID" value="AAK42946"/>
        <property type="gene ID" value="SSO2836"/>
    </dbReference>
    <dbReference type="GeneID" id="1452865"/>
    <dbReference type="KEGG" id="sso:SSO2836"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2922"/>
    <dbReference type="eggNOG" id="arCOG00925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q97V01"/>
    <dbReference type="PhylomeDB" id="Q97V01"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03219">
        <property type="entry name" value="ABC_Mj1267_LivG_branched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000421">
        <property type="entry name" value="Branched-chain amino acid ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051120">
        <property type="entry name" value="ABC_AA/LPS_Transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45772:SF5">
        <property type="entry name" value="BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45772">
        <property type="entry name" value="CONSERVED COMPONENT OF ABC TRANSPORTER FOR NATURAL AMINO ACIDS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="4"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00056071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="248" mass="27679" checksum="23F338D862364764" modified="2001-10-01" version="1">MSLLKLDSVSKYFGGIKALDSVTLEIENSKFTLLIGPNGSGKSTLINVVTGIYKPDSGSIFLEDRNITGKKPHELYKLGIVRTFQNPQIFPNLSVLDNVILGISPIKGESILRSLFKPIWLREEEELAEKAYNILKIVKLDRLWDRPARELSGGQLKLLEIARSLMTRPRLLVMDEPLAGVNPQLAKEILNILTDLKRDIGIFVVEHRLDIVANYADYVYAMFMGKVIAKGSVNDVLNNPTVVDAYLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V27</accession>
    <name>Q97V27_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Medium-chain-fatty-acid--CoA ligase (AlkK-3)</fullName>
            <ecNumber evidence="7">6.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">alkK-3</name>
        <name evidence="7" type="ordered locus">SSO2808</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.-" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99458">
        <property type="entry name" value="A99458"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990052.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V27"/>
    <dbReference type="FunCoup" id="Q97V27">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2808"/>
    <dbReference type="PaxDb" id="273057-SSO2808"/>
    <dbReference type="EnsemblBacteria" id="AAK42920">
        <property type="protein sequence ID" value="AAK42920"/>
        <property type="gene ID" value="SSO2808"/>
    </dbReference>
    <dbReference type="GeneID" id="1452839"/>
    <dbReference type="KEGG" id="sso:SSO2808"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2894"/>
    <dbReference type="eggNOG" id="arCOG00856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_5_2"/>
    <dbReference type="InParanoid" id="Q97V27"/>
    <dbReference type="PhylomeDB" id="Q97V27"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12119">
        <property type="entry name" value="ttLC_FACS_AlkK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000008">
        <property type="entry name" value="2,3-dihydroxybenzoate-AMP ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859:SF4">
        <property type="entry name" value="BUTANOATE--COA LIGASE AAE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="23"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="412"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="507" mass="57130" checksum="48CC28A6B3E982A0" modified="2001-10-01" version="1">MDSTIMDYNLNIRSIFWRIEKLYKDREIISRTYEGIVHYTYSDFALRVRKLATFLKTNGIAGEAVASIAWNTHRHLELYFAVPLLGGVLHTVNVRFHESEMDYVIKSMGDKAIFMDKDINYKNSTLPTFIFDERYDEDVNSQESTSDFPDVDERQGAIACFTSGTTGKPKGVIYSHRSVFIHSLSLLAADALGISSSDTVMPIVPMFHISAWDLPFSSLMTGAKLVLPGPRPKAEDIVKLIKEFKVTVGVGAPTVWIDVVNYVERENVDLPLKVVVTGGAEPPLGLIKKLKELGVKTYHAWGMTETEAIATVNKSDNIERMSEQGYPIPAFEIALMGPEGNELPWDGKSTGELVARGAFVTKKYLNDKSDAQTKWFRTGDVARINPDGSVKVIDRLKDLIKSGGEWISSVDLENAIMSYEKVLEAVVVGVKDERWGERPIALVVKKPGMDVNEYEIIEYLKSLNRFPKWWLPDKIIFVDSIPKTSTGKLDKKLVREQLRSMLEEKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V33</accession>
    <name>Q97V33_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2799</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90457">
        <property type="entry name" value="A90457"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923976.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V33"/>
    <dbReference type="STRING" id="273057.SSO2799"/>
    <dbReference type="PaxDb" id="273057-SSO2799"/>
    <dbReference type="EnsemblBacteria" id="AAK42912">
        <property type="protein sequence ID" value="AAK42912"/>
        <property type="gene ID" value="SSO2799"/>
    </dbReference>
    <dbReference type="GeneID" id="1452831"/>
    <dbReference type="KEGG" id="sso:SSO2799"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2885"/>
    <dbReference type="eggNOG" id="arCOG08321">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_929442_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V33"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="299" mass="34390" checksum="046E452F108CE98C" modified="2001-10-01" version="1">MIELQRKFFLNLSKLIKQGFHPVLLLNGCQKRVLPVMKIISSNGDLRGDLKIKLCIRMGIQEDKICEFFYPSTREITYEKEISTSKGNGLLLASSEGLLLVDVIYPRRNNEILRATLSNLITTWGVEWYEIEIKDDMVGFVWGFTDRRYMEVKEGMSVGMINRPGGMLPVKLLYKALNGNIEYLERRGIDINYVYELAEETFNRATKILKLNSNVLPINITRMGINNMYSLFPNYSLKIQLPVPWYSEIMKEIAPLIQDPLQSELLVLVIGEKREVEDALQEAEKQGFPSFLVGKVSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V94</accession>
    <name>Q97V94_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2741</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90449">
        <property type="entry name" value="D90449"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990274.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V94"/>
    <dbReference type="STRING" id="273057.SSO2741"/>
    <dbReference type="PaxDb" id="273057-SSO2741"/>
    <dbReference type="EnsemblBacteria" id="AAK42851">
        <property type="protein sequence ID" value="AAK42851"/>
        <property type="gene ID" value="SSO2741"/>
    </dbReference>
    <dbReference type="GeneID" id="1452770"/>
    <dbReference type="KEGG" id="sso:SSO2741"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2820"/>
    <dbReference type="eggNOG" id="arCOG08515">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514681_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V94"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="177" mass="20572" checksum="EADA30228A225BD5" modified="2001-10-01" version="1">MYCLDDRKRIPANVVCSSYNFKIRAIVNGRPYMASYEELWNFPLDGLNSIHLFENLDYALRNDPFLSATSSIAIFVSEKEDIEGFVELILSKTLFEIRSIILVENPISKYGDSEVTLSSSSLYYSVSYRNDRLMIKAKDREGYYEINMKLHNFTFVSDYRELLRNVEKVGKSTRVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VB3</accession>
    <name>Q97VB3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO10975</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK67526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VB3"/>
    <dbReference type="PaxDb" id="273057-SSO10975"/>
    <dbReference type="EnsemblBacteria" id="AAK67526">
        <property type="protein sequence ID" value="AAK67526"/>
        <property type="gene ID" value="SSO10975"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO10975"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2695948_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VB3"/>
    <dbReference type="PhylomeDB" id="Q97VB3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK67526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="73" mass="8039" checksum="C1E4321D7D04F97E" modified="2001-10-01" version="1">MGFKVRLVTLDTGFYTVEVLKFISQFKYVIGVPVEDVKAFSNKSFGRILSIVSATIATRTLSIRVIGEGHVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VN2</accession>
    <name>Q97VN2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methylisocitrate lyase</fullName>
            <ecNumber evidence="6">4.1.3.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">prpB</name>
        <name evidence="7" type="ordered locus">SSO2587</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2-methylisocitrate to yield pyruvate and succinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate</text>
            <dbReference type="Rhea" id="RHEA:16809"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57429"/>
            <dbReference type="EC" id="4.1.3.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Organic acid metabolism; propanoate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.30" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90431">
        <property type="entry name" value="H90431"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989295.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VN2"/>
    <dbReference type="FunCoup" id="Q97VN2">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2587"/>
    <dbReference type="PaxDb" id="273057-SSO2587"/>
    <dbReference type="EnsemblBacteria" id="AAK42711">
        <property type="protein sequence ID" value="AAK42711"/>
        <property type="gene ID" value="SSO2587"/>
    </dbReference>
    <dbReference type="GeneID" id="1454038"/>
    <dbReference type="GeneID" id="44128317"/>
    <dbReference type="KEGG" id="sso:SSO2587"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2668"/>
    <dbReference type="eggNOG" id="arCOG00581">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027389_3_2_2"/>
    <dbReference type="InParanoid" id="Q97VN2"/>
    <dbReference type="PhylomeDB" id="Q97VN2"/>
    <dbReference type="UniPathway" id="UPA00946"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046421">
        <property type="term" value="F:methylisocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019629">
        <property type="term" value="P:propionate catabolic process, 2-methylcitrate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000009">
        <property type="entry name" value="2-methylisocitrate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018523">
        <property type="entry name" value="Isocitrate_lyase_ph_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012695">
        <property type="entry name" value="PrpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02317">
        <property type="entry name" value="prpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905:SF5">
        <property type="entry name" value="CARBOXYVINYL-CARBOXYPHOSPHONATE PHOSPHORYLMUTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42905">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13714">
        <property type="entry name" value="PEP_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00161">
        <property type="entry name" value="ISOCITRATE_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="285" mass="32091" checksum="C201A02D296D9958" modified="2001-10-01" version="1">MSQVLREADFLMVPGVFNPFTAILAKKVGFKAVYLSGAALTSSYGLPDIGLITLDEVAEMIRRIKEVVDIPIIVDSDTGFGEAMNVYRTVRVLEKAGADAIQIEDQRMPKKCGHLEGKEVVEPLEMVQKIKAALKARRDALIIARVDSRGVIGLDDAIERAKIYLEAGADIIFPEALTSKEEFTKFAKEVKAPLLANMTEFGKTPYIKAQEFKEMGYKYVIFPVTIFRVAAKAMKDALEVLLREGTQINLLDKMINRQQQYEIIDYFFYERLDKELGSIKLVRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VX4</accession>
    <name>Q97VX4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sugar phosphate nucleotydyl transferase</fullName>
            <ecNumber evidence="2">2.7.7.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.-" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99420">
        <property type="entry name" value="A99420"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923884.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VX4"/>
    <dbReference type="FunCoup" id="Q97VX4">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2479"/>
    <dbReference type="PaxDb" id="273057-SSO2479"/>
    <dbReference type="EnsemblBacteria" id="AAK42616">
        <property type="protein sequence ID" value="AAK42616"/>
        <property type="gene ID" value="SSO2479"/>
    </dbReference>
    <dbReference type="GeneID" id="84057816"/>
    <dbReference type="KEGG" id="sso:SSO2479"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2558"/>
    <dbReference type="eggNOG" id="arCOG00663">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_2_2_2"/>
    <dbReference type="InParanoid" id="Q97VX4"/>
    <dbReference type="PhylomeDB" id="Q97VX4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="237" mass="27388" checksum="2A4A3A00DEE30C31" modified="2001-10-01" version="1">MYVMHAVILAGGYGKRLRPLTDDRPKPLIEVAGRPIIEWQISWLKQFGITSFVILTGYKWEVLIKWLSENEKRLGISTYFSIEEEPLGTGGALRKVERLLSTENTFIVLNGDIITNLDISKLKISNENVMTMSLVPLKSPYGIVETKDDKIIDFKEKPILENYWINAGVYLMRKEIFKYLPEKGDMEKLTFPKLAKESLLIGIKYYDVYWRSIDTIKDIEEVSEDLIKMKNGLSSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VY0</accession>
    <name>Q97VY0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glucan 1,4 alpha glucosidase (Glucoamylase)</fullName>
            <ecNumber evidence="2">3.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.2.1.3" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90419">
        <property type="entry name" value="C90419"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923878.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VY0"/>
    <dbReference type="SMR" id="Q97VY0"/>
    <dbReference type="FunCoup" id="Q97VY0">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2473"/>
    <dbReference type="CAZy" id="GH15">
        <property type="family name" value="Glycoside Hydrolase Family 15"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2473"/>
    <dbReference type="EnsemblBacteria" id="AAK42610">
        <property type="protein sequence ID" value="AAK42610"/>
        <property type="gene ID" value="SSO2473"/>
    </dbReference>
    <dbReference type="GeneID" id="1453937"/>
    <dbReference type="KEGG" id="sso:SSO2473"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2550"/>
    <dbReference type="eggNOG" id="arCOG03285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028187_0_0_2"/>
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        <property type="entry name" value="6hp_glycosidase-like_sf"/>
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        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
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        <property type="entry name" value="GH15-like"/>
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    <dbReference type="PANTHER" id="PTHR31616">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
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        <location>
            <begin position="248"/>
            <end position="601"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="612" mass="70939" checksum="235FF53F614CA9C6" modified="2001-10-01" version="1"> </sequence>
</entry>
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    <accession>Q97VZ7</accession>
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        <recommendedName>
            <fullName evidence="13">Protein phosphatase</fullName>
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        <name evidence="5" type="ordered locus">SSO2453</name>
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        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7 8 9" key="2">
        <citation type="journal article" date="2007" name="Proteins" volume="66" first="996" last="1003">
            <title>Enzyme-substrate interactions revealed by the crystal structures of the archaeal Sulfolobus PTP-fold phosphatase and its phosphopeptide complexes.</title>
            <authorList>
                <person name="Chu H.M."/>
                <person name="Wang A.H."/>
            </authorList>
            <dbReference type="PubMed" id="17173287"/>
            <dbReference type="DOI" id="10.1002/prot.21262"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.66 ANGSTROMS)</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="journal article" date="2011" name="J. Biomol. Struct. Dyn." volume="29" first="267" last="282">
            <title>Terpyridine platinum(II) complexes inhibit cysteine proteases by binding to active-site cysteine.</title>
            <authorList>
                <person name="Lo Y.C."/>
                <person name="Su W.C."/>
                <person name="Ko T.P."/>
                <person name="Wang N.C."/>
                <person name="Wang A.H."/>
            </authorList>
            <dbReference type="PubMed" id="21875148"/>
            <dbReference type="DOI" id="10.1080/073911011010524993"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS)</scope>
    </reference>
    <reference evidence="11 12" key="4">
        <citation type="journal article" date="2021" name="Biochemistry" volume="60" first="2888" last="2901">
            <title>Significant Loop Motions in the SsoPTP Protein Tyrosine Phosphatase Allow for Dual General Acid Functionality.</title>
            <authorList>
                <person name="Pinkston J."/>
                <person name="Jo J."/>
                <person name="Olsen K.J."/>
                <person name="Comer D."/>
                <person name="Glaittli C.A."/>
                <person name="Loria J.P."/>
                <person name="Johnson S.J."/>
                <person name="Hengge A.C."/>
            </authorList>
            <dbReference type="PubMed" id="34496202"/>
            <dbReference type="DOI" id="10.1021/acs.biochem.1c00365"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.05 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42593.1"/>
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    </dbReference>
    <dbReference type="PIR" id="B90417">
        <property type="entry name" value="B90417"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A=1-161"/>
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    <dbReference type="PDBsum" id="2I6P"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000157">
        <property type="entry name" value="Protein-tyrosine phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
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        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22785">
        <property type="entry name" value="Tc-R-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="7 8 9" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="76"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="2DXP"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="2I6I"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="2I6J"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="3RO1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="11">
        <source>
            <dbReference type="PDB" id="7MPC"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="12">
        <source>
            <dbReference type="PDB" id="7MPD"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18412" checksum="2876B6FE4E6BA942" modified="2001-10-01" version="1">MYWVRRKTIGGSGLPYTENEILEWRKEGVKRVLVLPEDWEIEESWGDKDYYLSILKKNGLQPLHIPIPDGGVPSDSQFLTIMKWLLSEKEGNLVHCVGGIGRTGTILASYLILTEGLEVESAIDEVRLVRPGAVQTYEQEMFLLRVEGMRKSWLKNIYSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="138" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VZ8</accession>
    <name>Q97VZ8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KaiC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2452</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
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            <taxon>Thermoproteota</taxon>
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            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2009" name="J. Mol. Biol." volume="389" first="661" last="673">
            <title>Structural and functional characterisation of a conserved archaeal RadA paralog with antirecombinase activity.</title>
            <authorList>
                <person name="McRobbie A.M."/>
                <person name="Carter L.G."/>
                <person name="Kerou M."/>
                <person name="Liu H."/>
                <person name="McMahon S.A."/>
                <person name="Johnson K.A."/>
                <person name="Oke M."/>
                <person name="Naismith J.H."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="19414020"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2009.04.060"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 1-235 IN COMPLEX WITH ZN(2+)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99417">
        <property type="entry name" value="A99417"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923860.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2W0M">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-235"/>
    </dbReference>
    <dbReference type="PDBsum" id="2W0M"/>
    <dbReference type="AlphaFoldDB" id="Q97VZ8"/>
    <dbReference type="SMR" id="Q97VZ8"/>
    <dbReference type="STRING" id="273057.SSO2452"/>
    <dbReference type="PaxDb" id="273057-SSO2452"/>
    <dbReference type="EnsemblBacteria" id="AAK42592">
        <property type="protein sequence ID" value="AAK42592"/>
        <property type="gene ID" value="SSO2452"/>
    </dbReference>
    <dbReference type="GeneID" id="1453919"/>
    <dbReference type="KEGG" id="sso:SSO2452"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2530"/>
    <dbReference type="eggNOG" id="arCOG01171">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023669_2_0_2"/>
    <dbReference type="InParanoid" id="Q97VZ8"/>
    <dbReference type="PhylomeDB" id="Q97VZ8"/>
    <dbReference type="EvolutionaryTrace" id="Q97VZ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01124">
        <property type="entry name" value="KaiC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022443">
        <property type="entry name" value="KaiC-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03881">
        <property type="entry name" value="KaiC_arch_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF2">
        <property type="entry name" value="PROTEIN GVPD 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01874">
        <property type="entry name" value="DNAREPAIRADA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="6" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="KaiC" evidence="3">
        <location>
            <begin position="3"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="2W0M"/>
        </source>
    </evidence>
    <sequence length="262" mass="30288" checksum="E3D1974270FDA974" modified="2001-10-01" version="1">MVSRLSTGILDFDKLIQGGIPQGFFIALTGEPGTGKTIFSLHFIAKGLRDGDPCIYVTTEESRDSIIRQAKQFNWDFEEYIEKKLIIIDALMKEKEDQWSLVNLTPEELVNKVIEAKQKLGYGKARLVIDSVSALFLDKPAMARKISYYLKRVLNKWNFTIYATSQYAITTSQAFGFGVEHVADGIIRFRRMIRNGELHRYILIEKMRQTDHDKHVWEIDIVNGKGIVLKGRLEERREDYTLPEKVKRKIIESGKKTEEELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W06</accession>
    <name>Q97W06_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alcohol dehydrogenase (Zn containing) (Adh-7)</fullName>
            <ecNumber evidence="6">1.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">adh-7</name>
        <name evidence="6" type="ordered locus">SSO2441</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.1" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99416">
        <property type="entry name" value="A99416"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993129.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W06"/>
    <dbReference type="FunCoup" id="Q97W06">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2441"/>
    <dbReference type="PaxDb" id="273057-SSO2441"/>
    <dbReference type="EnsemblBacteria" id="AAK42584">
        <property type="protein sequence ID" value="AAK42584"/>
        <property type="gene ID" value="SSO2441"/>
    </dbReference>
    <dbReference type="GeneID" id="1453911"/>
    <dbReference type="KEGG" id="sso:SSO2441"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2522"/>
    <dbReference type="eggNOG" id="arCOG01457">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_2_2"/>
    <dbReference type="InParanoid" id="Q97W06"/>
    <dbReference type="PhylomeDB" id="Q97W06"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030554">
        <property type="term" value="F:adenyl nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043168">
        <property type="term" value="F:anion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008743">
        <property type="term" value="F:L-threonine 3-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006566">
        <property type="term" value="P:threonine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF4">
        <property type="entry name" value="D-ARABINOSE 1-DEHYDROGENASE (NADP(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="5">
        <location>
            <begin position="24"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="4">
        <location>
            <begin position="167"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="328" mass="35928" checksum="04BE0B33ACAA735F" modified="2001-10-01" version="1">MLGAILKNFGEELSLEDVREPSNGIVLKVLATGLCHGDLHVINGDWRYEIKVELPKILGHEIVGEVVKGNGTFKEGDKVLVYNSIGCGKCKYCASKKYQYCEKVKIIGLNINGGFAEYVSVPSDNILLRVDGEPVKLAPLADAGITAYSSAKGITNEDSVLIIGTGAVALLALQILKLKGARVTVIGHNKNKLEKAKELGADEVINIKYDSTIASEVKESKFDYIIDYVGSDYTLSDSMWLLNREGELRIIGEFGGTITVPEQLLVLKGLKVRGILYGSFDDLISVYKLYNEGKIRPIVNTYKLRDINKAIRDLRDGRVIGRAVIVPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W13</accession>
    <name>Q97W13_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Carbon monoxide dehydrogenase, medium chain. (CutB-1)</fullName>
            <ecNumber evidence="5">1.2.7.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cutB-1</name>
        <name evidence="5" type="ordered locus">SSO2434</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.4" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99415">
        <property type="entry name" value="B99415"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989389.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W13"/>
    <dbReference type="STRING" id="273057.SSO2434"/>
    <dbReference type="PaxDb" id="273057-SSO2434"/>
    <dbReference type="EnsemblBacteria" id="AAK42577">
        <property type="protein sequence ID" value="AAK42577"/>
        <property type="gene ID" value="SSO2434"/>
    </dbReference>
    <dbReference type="GeneID" id="1453904"/>
    <dbReference type="KEGG" id="sso:SSO2434"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2513"/>
    <dbReference type="eggNOG" id="arCOG01926">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058050_3_0_2"/>
    <dbReference type="InParanoid" id="Q97W13"/>
    <dbReference type="PhylomeDB" id="Q97W13"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:anaerobic carbon-monoxide dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036683">
        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051312">
        <property type="entry name" value="Diverse_Substr_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053586">
        <property type="entry name" value="Glyceraldehyde_DH_medium"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041019">
        <property type="entry name" value="glyceraldDH_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42659">
        <property type="entry name" value="XANTHINE DEHYDROGENASE SUBUNIT C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42659:SF2">
        <property type="entry name" value="XANTHINE DEHYDROGENASE SUBUNIT C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03450">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00941">
        <property type="entry name" value="FAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01092">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55447">
        <property type="entry name" value="CO dehydrogenase flavoprotein C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="278" mass="30744" checksum="0590DCCBB2006899" modified="2001-10-01" version="1">MYPPKFSYVIPDNVKEALEFLETHDDAKPLAGGHSLIPMLKLRIFRPSYLVEIRRLPELKYIKMEGNVVKIGPIVTHYDIIKANIPLLSETASKIADPQVRNMGTIGGSISHLDPSADYPAALIAMNAKVRIRSTKGERVENFSSFAKDMFTPDLNPGELVTEIEVPLLKDYKFSYQKLERRAGDFAIVGVAVALKVNGDVIEDARIGLTAVNKTAVRASEAEKILLSGKISEKLIEEAATKAMDYANPTSDIRGSAEYKKKMVKVMTKRAILAALNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W49</accession>
    <name>Q97W49_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Bifunctional thiamine biosynthesis protein ThiDN</fullName>
            <ecNumber evidence="6">2.5.1.3</ecNumber>
            <ecNumber evidence="5">2.7.1.49</ecNumber>
            <ecNumber evidence="7">2.7.4.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">thiD-2</name>
        <name evidence="24" type="ordered locus">SSO2393</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:47848"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62890"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:47844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ATP = 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + ADP</text>
            <dbReference type="Rhea" id="RHEA:19893"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-amino-5-hydroxymethyl-2-methylpyrimidine + ATP = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23096"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16892"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.49"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>4-methyl-5-(2-phosphooxyethyl)-thiazole + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + H(+) = thiamine phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="18">Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">In the C-terminal section; belongs to the ThiN family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20">In the N-terminal section; belongs to the ThiD family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.3" evidence="6"/>
    <dbReference type="EC" id="2.7.1.49" evidence="5"/>
    <dbReference type="EC" id="2.7.4.7" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90410">
        <property type="entry name" value="E90410"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W49"/>
    <dbReference type="FunCoup" id="Q97W49">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2393"/>
    <dbReference type="PaxDb" id="273057-SSO2393"/>
    <dbReference type="EnsemblBacteria" id="AAK42540">
        <property type="protein sequence ID" value="AAK42540"/>
        <property type="gene ID" value="SSO2393"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2393"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2475"/>
    <dbReference type="eggNOG" id="arCOG00020">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035788_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W49"/>
    <dbReference type="PhylomeDB" id="Q97W49"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000015">
        <property type="entry name" value="Phosphomethylpyrimidine kinase (Hmp-phosphate kinase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000096">
        <property type="entry name" value="Phosphomethylpyrimidine kinase (ThiD-2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019293">
        <property type="entry name" value="ThiN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858:SF17">
        <property type="entry name" value="HYDROXYMETHYLPYRIMIDINE_PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10120">
        <property type="entry name" value="ThiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 24" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="8 24" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="22">
        <location>
            <begin position="25"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine-phosphate synthase ThiN" evidence="23">
        <location>
            <begin position="282"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00059241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00060674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00060834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00060992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00067160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF10120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="AAK42540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="456" mass="51661" checksum="855717C0641BA7A1" modified="2001-10-01" version="1">MGSSREYESEKTMIKPVGMSIAGLDTGNGAGGETDLRVFEVLGIHGVFAITAITAQSTKGIKDITVVNPEFLKKQIETLLEDFKVEAVKIGMIYTKEQFQVVNELLNDYFLVADPVLYAKDGTPLIRDIEEYKKIILPKVKVITPNIIEASAISGVKIEKESDVVIVCKKLRDSYNIPYVIIKGGHSKGDYSFDYMCNDEGLYKIGYKRLQAKDTHGTGSVFATALTAEYIKIRDLKLAFRKARDFVQTSIEYGLNIGKGIGPVNVSVEIMKKSMKYEAVEEMRRFADFAENNDRFWILIPEVQSNLAHSIKPEYVRDLNDIATFRGRIIRRWDKKVIVGHPVVFGNPTHTARMLLSLILKGKDSTCLMNIRYDDKIVESFKRIGYETIEINRELEPAHGEGKTMQWIIEYVSSEYGGIPNVIYDKGTKGKEAMIRFWTKNMEEMIEALDNLLKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W66</accession>
    <name>Q97W66_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1152 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2371</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90408">
        <property type="entry name" value="C90408"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989470.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W66"/>
    <dbReference type="STRING" id="273057.SSO2371"/>
    <dbReference type="PaxDb" id="273057-SSO2371"/>
    <dbReference type="EnsemblBacteria" id="AAK42522">
        <property type="protein sequence ID" value="AAK42522"/>
        <property type="gene ID" value="SSO2371"/>
    </dbReference>
    <dbReference type="GeneID" id="1453849"/>
    <dbReference type="KEGG" id="sso:SSO2371"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2456"/>
    <dbReference type="eggNOG" id="arCOG04164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069296_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W66"/>
    <dbReference type="PhylomeDB" id="Q97W66"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010581">
        <property type="entry name" value="DUF1152"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06626">
        <property type="entry name" value="DUF1152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029122">
        <property type="entry name" value="DUF1152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="36781" checksum="C41A11630DCC936D" modified="2001-10-01" version="1">MKAFVFGIGGGGDVVSAIVAYNYLRKLGYDVLLGSVVWERYVEDPIPGPICFDNIRNGVLVNQGLYKVNRDTYAIRGNRRVVPQLVRAVRALNLGEAYGICNKDGVIGLYSSLKEFVNKEKIDLIVGVDAGGDVLAKGCEETLGSPLIDFISLASLVKLEEDGYNVMLGVIGSGSDGELQYDYFLKRISEIASLNGLLDIKGYDRETANLVGRILNEVTTEASKIPFDAFKGLYGEITIRNGTRKVLVTPISAVMFFLDPIKIAETSPLYSLVKDSISIDDAKKNLNEVGIYTEYNFEEDLYNEFGLNASHASAYDVDRIRVEGKRKLGGVKIKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W78</accession>
    <name>Q97W78_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Succinate dehydrogenase subunit B (SdhB)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">sdhB</name>
        <name evidence="19" type="ordered locus">SSO2357</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90406">
        <property type="entry name" value="G90406"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989494.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W78"/>
    <dbReference type="FunCoup" id="Q97W78">
        <property type="interactions" value="335"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2357"/>
    <dbReference type="PaxDb" id="273057-SSO2357"/>
    <dbReference type="EnsemblBacteria" id="AAK42510">
        <property type="protein sequence ID" value="AAK42510"/>
        <property type="gene ID" value="SSO2357"/>
    </dbReference>
    <dbReference type="GeneID" id="1453837"/>
    <dbReference type="KEGG" id="sso:SSO2357"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2444"/>
    <dbReference type="eggNOG" id="arCOG00962">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044838_3_1_2"/>
    <dbReference type="InParanoid" id="Q97W78"/>
    <dbReference type="PhylomeDB" id="Q97W78"/>
    <dbReference type="BRENDA" id="1.3.5.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000003">
        <property type="entry name" value="Succinate dehydrogenase iron-sulfur subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009226">
        <property type="entry name" value="PRK12576.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43551">
        <property type="entry name" value="FUMARATE REDUCTASE IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43551:SF2">
        <property type="entry name" value="FUMARATE REDUCTASE IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="18">
        <location>
            <begin position="146"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="18">
        <location>
            <begin position="199"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AAK42510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="316" mass="36792" checksum="ABEAAC2D7EB2A2AE" modified="2001-10-01" version="1">MTQSQEEEVILKVKRFNPEKGYWWAEYKLKVDRFTQFTEALRRIKSEQDPTLSYRASCHMAVCGSCGMKINGEPRLACKTLVLDVVKKYNNNVITIEPMDYFKPIKDLIVDWDEFYERMFKIKPRLYQAKEVLEGKAEHRLKPEDQRELWKFAQCIWCGLCVSACPAVVIDQQFLGPAAHAKGYRFLADPRDTITEERMKILIDSSWRCTYCYQCFNVCPRDIEPVTAIKKTRSFTKTYKDKSEIAERGEKHVEAIHESILKTGKLAEAPVYLKTYGVLQSLVDLVYMSKTGKLKYALVQERPVQNINEIKKIIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WF8</accession>
    <name>Q97WF8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2265</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90396">
        <property type="entry name" value="F90396"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991525.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WF8"/>
    <dbReference type="SMR" id="Q97WF8"/>
    <dbReference type="FunCoup" id="Q97WF8">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2265"/>
    <dbReference type="PaxDb" id="273057-SSO2265"/>
    <dbReference type="EnsemblBacteria" id="AAK42429">
        <property type="protein sequence ID" value="AAK42429"/>
        <property type="gene ID" value="SSO2265"/>
    </dbReference>
    <dbReference type="GeneID" id="1453756"/>
    <dbReference type="KEGG" id="sso:SSO2265"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2356"/>
    <dbReference type="eggNOG" id="arCOG01226">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043725_1_0_2"/>
    <dbReference type="InParanoid" id="Q97WF8"/>
    <dbReference type="PhylomeDB" id="Q97WF8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052040">
        <property type="entry name" value="GTPase/Isobutyryl-CoA_mutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005129">
        <property type="entry name" value="GTPase_ArgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00750">
        <property type="entry name" value="lao"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43087:SF1">
        <property type="entry name" value="LAO_AO TRANSPORT SYSTEM ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43087">
        <property type="entry name" value="LYSINE/ARGININE/ORNITHINE TRANSPORT SYSTEM KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03308">
        <property type="entry name" value="MeaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="48"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="315" mass="34727" checksum="C3A59B3B3C909B61" modified="2001-10-01" version="1">MLNNNLSKLIEEALNGNELAIARLLTKIEYMTDEGISALGTLSKRSGNAHVIGITGIPGSGKSTIIGGLIQEYVSRGHRVGVIVIDPSSPYTMGSFMGNRLRFQDKTMLKNVFVRSIGSRGYLGGISAEALMLTEALDGLGYDKIIIETVGAGQTDTEVEQSVHTILVVSIPGAGDDIQALKAGIMEIGDIYVLNKADRQDAELAFNTLKFAIDSAEISYRNGWKPLIVKTVAIKNEGIIELVNKIEDHLNYLKQKELFKKRIIDRRAKIVELIARRKIDEIISEIVKKNYNTIINEDITEAIPKVIHNVKELLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WK5</accession>
    <name>Q97WK5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2211</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90390">
        <property type="entry name" value="F90390"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992072.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WK5"/>
    <dbReference type="STRING" id="273057.SSO2211"/>
    <dbReference type="PaxDb" id="273057-SSO2211"/>
    <dbReference type="EnsemblBacteria" id="AAK42381">
        <property type="protein sequence ID" value="AAK42381"/>
        <property type="gene ID" value="SSO2211"/>
    </dbReference>
    <dbReference type="GeneID" id="1453708"/>
    <dbReference type="KEGG" id="sso:SSO2211"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2307"/>
    <dbReference type="eggNOG" id="arCOG00938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_777602_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WK5"/>
    <dbReference type="PhylomeDB" id="Q97WK5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023819">
        <property type="entry name" value="Pep-mod_rSAM_AF0577"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023867">
        <property type="entry name" value="Sulphatase_maturase_rSAM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04084">
        <property type="entry name" value="rSAM_AF0577"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43273">
        <property type="entry name" value="ANAEROBIC SULFATASE-MATURATING ENZYME HOMOLOG ASLB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43273:SF2">
        <property type="entry name" value="RADICAL SAM CORE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01104">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="7"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="350" mass="40314" checksum="A58045FD1324A01F" modified="2001-10-01" version="1">MLWLLMTTGKCNLKCDYCGGSFPDNVVPYGIKYDINSVKKLIEKDKDSTIIFYGGEPLLNYKFVMAVLDNVRAKRYGIQTNGIAVRLLPERYWRKINVALLSIDGREEITNKHRGKGIYKAVLRSAKYLKDLGVETIARMTVTQDTDIFTDVMHLIDLGLFDKVHWQLDVIWSEEWDFKSWSFSYLNGIKRLTDYFLSELRKGRVVKIIPILGVISAHFFEGYKGSPCGAGYSSVTVTPDGRILSCPIAVREKWAELGNVNLGYRLLEDPLPDDCKRCEFKRYCGGRCLYAQKERYWGEEGFKAVDEVNKEYLRTVLSIIPEIERLIRDGYIRLSDLYYNPILDSTEIIP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-31" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WK8</accession>
    <name>NUCS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease NucS</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nucS</name>
        <name type="ordered locus">SSO2208</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cleaves both 3' and 5' ssDNA extremities of branched DNA structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NucS endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90390">
        <property type="entry name" value="C90390"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992078.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WK8"/>
    <dbReference type="SMR" id="Q97WK8"/>
    <dbReference type="STRING" id="273057.SSO2208"/>
    <dbReference type="PaxDb" id="273057-SSO2208"/>
    <dbReference type="EnsemblBacteria" id="AAK42378">
        <property type="protein sequence ID" value="AAK42378"/>
        <property type="gene ID" value="SSO2208"/>
    </dbReference>
    <dbReference type="GeneID" id="1453705"/>
    <dbReference type="GeneID" id="44127943"/>
    <dbReference type="KEGG" id="sso:SSO2208"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2304"/>
    <dbReference type="eggNOG" id="arCOG01304">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069350_1_0_2"/>
    <dbReference type="InParanoid" id="Q97WK8"/>
    <dbReference type="PhylomeDB" id="Q97WK8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22341">
        <property type="entry name" value="NucS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.180.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00722">
        <property type="entry name" value="NucS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002793">
        <property type="entry name" value="Endonuclease_NucS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048301">
        <property type="entry name" value="NucS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048302">
        <property type="entry name" value="NucS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049173">
        <property type="entry name" value="NucS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003270">
        <property type="entry name" value="PRK04247.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38814">
        <property type="entry name" value="ENDONUCLEASE NUCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38814:SF1">
        <property type="entry name" value="ENDONUCLEASE NUCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01939">
        <property type="entry name" value="NucS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21003">
        <property type="entry name" value="NucS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000155700" description="Endonuclease NucS">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00722"/>
        </source>
    </evidence>
    <sequence length="236" mass="27265" checksum="6930125F102BBAC8" modified="2001-10-01" version="1">MYSVLLNPSNTEIYSFLTDRIYRELIVIFATCKVNYKGRAESVASESPRLIILKPDGTVIIHESVKREPLNWQPPGTKIEIMNDYPLKIVAQRNRPKEVIEIDLKEVFYITSAEVKEGDFTIKGREIDIVNTIIQNPSMIEEGFVPLTREYNTPYGKIDLIGLDKKGNFVIIEVKRSKAQLNAISQLYRYYLHMREIKGDKIRGILVAPDLTIHARELLQKLGLEFIRYDIKNYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WQ5</accession>
    <name>Q97WQ5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ChsH2 rubredoxin-like zinc ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90373">
        <property type="entry name" value="H90373"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988837.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WQ5"/>
    <dbReference type="STRING" id="273057.SSO2063"/>
    <dbReference type="PaxDb" id="273057-SSO2063"/>
    <dbReference type="EnsemblBacteria" id="AAK42247">
        <property type="protein sequence ID" value="AAK42247"/>
        <property type="gene ID" value="SSO2063"/>
    </dbReference>
    <dbReference type="GeneID" id="1453574"/>
    <dbReference type="KEGG" id="sso:SSO2063"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2142"/>
    <dbReference type="eggNOG" id="arCOG01287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119412_2_0_2"/>
    <dbReference type="InParanoid" id="Q97WQ5"/>
    <dbReference type="PhylomeDB" id="Q97WQ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="ChsH2_Znr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF4">
        <property type="entry name" value="DUF35 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="1">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="165" mass="19095" checksum="F44BF5772AB2B558" modified="2001-10-01" version="1">MVTPLKEEELSKHYIISYKPNAKYAYTAGQAQSKYLLGLKEGKIYGRKCNKCGRIHVPPKMYCEECFRPTDEWVEVKDEGIVMTAVASFISWTRAKLEEPEIVGVIRLLPSNDRDFVYPGIFHRICASYEQVKSMEIIGKRVKAVWRPKEERKGSIEDIQCFKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WT2</accession>
    <name>Q97WT2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="D90370"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WT2"/>
    <dbReference type="PaxDb" id="273057-SSO2032"/>
    <dbReference type="EnsemblBacteria" id="AAK42219">
        <property type="protein sequence ID" value="AAK42219"/>
        <property type="gene ID" value="SSO2032"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2032"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2114"/>
    <dbReference type="eggNOG" id="arCOG07264">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1412422_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WT2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="176" mass="19930" checksum="8C07DBA8891F501E" modified="2001-10-01" version="1">MHIHMDKKILIFIVLIVIISFALIMVYPSKDLLFSPQEAEAIFGGNWEVLQNNTNLKSIGTITIYYANSTNLTTQHSLEVESIEEEVLVGDVNNTKVEMTIVIVKFSSNSSWSEIFGNNTNYYKYNGYYITYSATSFTYPKTIIVAYKGSTLVEITLDGYQASFAQVKEILSYLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WU2</accession>
    <name>Q97WU2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ring oxydation complex/ phenylacetic acid degradation related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2022</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99369">
        <property type="entry name" value="A99369"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923730.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WU2"/>
    <dbReference type="STRING" id="273057.SSO2022"/>
    <dbReference type="PaxDb" id="273057-SSO2022"/>
    <dbReference type="EnsemblBacteria" id="AAK42208">
        <property type="protein sequence ID" value="AAK42208"/>
        <property type="gene ID" value="SSO2022"/>
    </dbReference>
    <dbReference type="GeneID" id="7805861"/>
    <dbReference type="KEGG" id="sso:SSO2022"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2098"/>
    <dbReference type="eggNOG" id="arCOG07233">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067515_2_0_2"/>
    <dbReference type="InParanoid" id="Q97WU2"/>
    <dbReference type="PhylomeDB" id="Q97WU2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048846">
        <property type="entry name" value="PaaX-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012906">
        <property type="entry name" value="PaaX-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013225">
        <property type="entry name" value="PaaX_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011965">
        <property type="entry name" value="PaaX_trns_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30319">
        <property type="entry name" value="PHENYLACETIC ACID REGULATOR-RELATED TRANSCRIPTIONAL REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30319:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PAAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07848">
        <property type="entry name" value="PaaX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08223">
        <property type="entry name" value="PaaX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20803">
        <property type="entry name" value="PaaX_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF020623">
        <property type="entry name" value="PaaX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcriptional repressor PaaX-like N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Transcriptional repressor PaaX-like central Cas2-like" evidence="3">
        <location>
            <begin position="89"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Transcriptional repressor PaaX-like C-terminal" evidence="2">
        <location>
            <begin position="175"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="266" mass="31094" checksum="22C52FF1560BEA18" modified="2001-10-01" version="1">MKIQSLFFTLYGDYVKDSGGTISSKSLIVIFKEFGFSEGAIRAGLHRMKKAGLIVGIKGENRKVSYKLSEKGMLRLLEGTRRVYEKVRRRWDNKWRIVVYNIPENNRELRDKLRRELKWLGFGMLAQSTWISPNPIEDTLKNFINDHYGSSNGIQVDIFVANYLGEPKGLVEKCWNLSEVEQAYRAFLEKWTGVLEKVSSLKSNEAFVTRILLVHEYRKFLNIDPDLPEDLLPPNWIGYTAYDLFMKLREELTPKANEFFYKVYEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WV5</accession>
    <name>Q97WV5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1048</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90367">
        <property type="entry name" value="D90367"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WV5"/>
    <dbReference type="STRING" id="273057.SSO2007"/>
    <dbReference type="PaxDb" id="273057-SSO2007"/>
    <dbReference type="EnsemblBacteria" id="AAK42195">
        <property type="protein sequence ID" value="AAK42195"/>
        <property type="gene ID" value="SSO2007"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2007"/>
    <dbReference type="eggNOG" id="arCOG02130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1943955_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WV5"/>
    <dbReference type="PhylomeDB" id="Q97WV5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="129" mass="14934" checksum="E4F406D7AB579945" modified="2001-10-01" version="1">MEGEALFFLDESRIVISTAIRKVLAKIYSKPVMHVNMGFSSLHVFLAINAWTGEVVVGILRRTNSESLKYFLRYFKMVMGGSTWLWIIILLTRLGLRFVAGRAFIPYLLLPIRFSLIWQRRSSSPSRYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X32</accession>
    <name>Q97X32_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO9250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90357">
        <property type="entry name" value="E90357"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923653.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X32"/>
    <dbReference type="PaxDb" id="273057-SSO9250"/>
    <dbReference type="EnsemblBacteria" id="AAK42116">
        <property type="protein sequence ID" value="AAK42116"/>
        <property type="gene ID" value="SSO9250"/>
    </dbReference>
    <dbReference type="GeneID" id="1453443"/>
    <dbReference type="KEGG" id="sso:SSO9250"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1990"/>
    <dbReference type="eggNOG" id="arCOG08588">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG09928">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134214_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X32"/>
    <dbReference type="PhylomeDB" id="Q97X32"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase InsH N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="98" mass="11605" checksum="36B719912C530378" modified="2001-10-01" version="1">MLKVDKVFPWETFRGKLESPYSKKPKWDVILLLKVLLIKFVYEISWLEGEIRDSKMFMKFLGGKVHQKSILLLQETVVDEGERMWTTLMTQQGLRQGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X37</accession>
    <name>Q97X37_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1918</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90356">
        <property type="entry name" value="H90356"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990344.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X37"/>
    <dbReference type="STRING" id="273057.SSO1918"/>
    <dbReference type="PaxDb" id="273057-SSO1918"/>
    <dbReference type="EnsemblBacteria" id="AAK42111">
        <property type="protein sequence ID" value="AAK42111"/>
        <property type="gene ID" value="SSO1918"/>
    </dbReference>
    <dbReference type="GeneID" id="54123520"/>
    <dbReference type="KEGG" id="sso:SSO1918"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1983"/>
    <dbReference type="eggNOG" id="arCOG03166">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041137_3_0_2"/>
    <dbReference type="InParanoid" id="Q97X37"/>
    <dbReference type="PhylomeDB" id="Q97X37"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004256">
        <property type="entry name" value="DUF234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34704">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34704:SF1">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03008">
        <property type="entry name" value="DUF234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="DUF234" evidence="2">
        <location>
            <begin position="295"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="49612" checksum="A3CA9C62C5B122FD" modified="2001-10-01" version="1">MIFMNMVNRERELQALKKRLSSNDLELIIIYGRRRIGKTFLIINAVNGYDFIYYLATEKNNLRKFKELTESKYEDIKYVKEDWEAIFHYLRDKIIIIDEFPYMIKEDESILSTFQKIVDETLKGSKTKLILLGSSISMMEDAISYKSPLYGRRTASIELKELKFKDLKGFNFSLIDAIHVYGFAGGVPFYLTKAKTPFLKWINAELKEVDSFIKDEIDFMLRYEFSEIGTYKEILHAISSGKNTLGEIKDFVRISGDVSSYLNKLEKIGIIGKEFPYKMKKGARYYIKDNFTLFWFTFIYPNLSLIEEGIYEIKEDEYNVYLGRIFEKIAREYVEDVYKVKVERLWFKDVEIDIYGDGIAGECKWSEGINGEKVLYELKRKVEDLGLDVKKYIIFAKSFSKTNEEAEFIDLKKLEQWYRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XC6</accession>
    <name>Q97XC6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sulfurtransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cysA-2</name>
        <name evidence="4" type="ordered locus">SSO1817</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90344">
        <property type="entry name" value="H90344"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992891.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XC6"/>
    <dbReference type="FunCoup" id="Q97XC6">
        <property type="interactions" value="183"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1817"/>
    <dbReference type="PaxDb" id="273057-SSO1817"/>
    <dbReference type="EnsemblBacteria" id="AAK42015">
        <property type="protein sequence ID" value="AAK42015"/>
        <property type="gene ID" value="SSO1817"/>
    </dbReference>
    <dbReference type="GeneID" id="1454794"/>
    <dbReference type="KEGG" id="sso:SSO1817"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1878"/>
    <dbReference type="eggNOG" id="arCOG02019">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031618_1_3_2"/>
    <dbReference type="InParanoid" id="Q97XC6"/>
    <dbReference type="PhylomeDB" id="Q97XC6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01449">
        <property type="entry name" value="TST_Repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051126">
        <property type="entry name" value="Thiosulfate_sulfurtransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43855">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43855:SF1">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00683">
        <property type="entry name" value="RHODANESE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="21"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="167"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="293" mass="33655" checksum="4CA1D4503A948B25" modified="2001-10-01" version="1">MSQTLQQGVIVDNKWVLEHLKDPNVRIVEVDYDPNTAYNVWHIPGSVLIRWREDLRHPVLRDFIEPSQFEKLMESKGISNNTTIVLYGDYNNWFAVYAFWLFKAYGHEDVRILNGGRTKWAKENLPTEQGPKETQYPKGSYKVKKVDWGNHRAFFWEILQKVTKGEIGKTTLLIDVRSPKEFTGEIRAPPEYADEQTQVGGHIPGAISFPWAQAVNPDTGEFKSVEELRRLVENVGITSDKEIITYCRIGERASHTWFVLKYLLGYPSVRVYDGSWAEWGNIVGAPVKKGAEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XD3</accession>
    <name>Q97XD3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF2249 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1812</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90344">
        <property type="entry name" value="A90344"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991466.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XD3"/>
    <dbReference type="STRING" id="273057.SSO1812"/>
    <dbReference type="PaxDb" id="273057-SSO1812"/>
    <dbReference type="EnsemblBacteria" id="AAK42008">
        <property type="protein sequence ID" value="AAK42008"/>
        <property type="gene ID" value="SSO1812"/>
    </dbReference>
    <dbReference type="GeneID" id="1454787"/>
    <dbReference type="KEGG" id="sso:SSO1812"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1870"/>
    <dbReference type="eggNOG" id="arCOG03002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1340782_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XD3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018720">
        <property type="entry name" value="DUF2249"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37694">
        <property type="entry name" value="SLR8022 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37694:SF1">
        <property type="entry name" value="SLR8022 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10006">
        <property type="entry name" value="DUF2249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2249" evidence="2">
        <location>
            <begin position="2"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-2" evidence="1">
        <location>
            <begin position="107"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="22550" checksum="4764C11C917F2294" modified="2001-10-01" version="1">MELDLRPIQPEYRHKLVLESFSKLKEGEELTVIADHYPSHLIQLLSGYIEKYKVEETANGDFVLKLTKKKGSTNKAIISHISEFRKTGDVFTPIPVLRKDEYGVILVFFKPGQYIPIHAPDSDLIFYVLQGQGKVSVDNREYEVHEGSIVIVPRGIKRGVKADTYMEAIHIVVPSPSPEDHEKVMKAAMNGIAEVDLRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XD6</accession>
    <name>Q97XD6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1809</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90343">
        <property type="entry name" value="F90343"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991469.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XD6"/>
    <dbReference type="STRING" id="273057.SSO1809"/>
    <dbReference type="PaxDb" id="273057-SSO1809"/>
    <dbReference type="EnsemblBacteria" id="AAK42005">
        <property type="protein sequence ID" value="AAK42005"/>
        <property type="gene ID" value="SSO1809"/>
    </dbReference>
    <dbReference type="GeneID" id="1454784"/>
    <dbReference type="KEGG" id="sso:SSO1809"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1867"/>
    <dbReference type="eggNOG" id="arCOG08434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1465182_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XD6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="177" mass="20915" checksum="B501144706DFC4E3" modified="2001-10-01" version="1">MGNTEIIDRAKSKLERFLGFSFPTPLVIETSIPFYGSYRYDVVIIGYFDNDEDALERTLIHELAHFVQDKICKPKFPYLHMLIEAIPLAFGIGKGKLLRYFQFVEGFATWIDEEIMGYRGDEYWFSVPKYYFKVYYKGAEIFRKMGKEKAIEFGLTNREFCKEPQRYKSLRTITISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XE9</accession>
    <name>Q97XE9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99342">
        <property type="entry name" value="A99342"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923596.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XE9"/>
    <dbReference type="PaxDb" id="273057-SSO1800"/>
    <dbReference type="EnsemblBacteria" id="AAK41992">
        <property type="protein sequence ID" value="AAK41992"/>
        <property type="gene ID" value="SSO1800"/>
    </dbReference>
    <dbReference type="GeneID" id="1454771"/>
    <dbReference type="KEGG" id="sso:SSO1800"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1852"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154343_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XE9"/>
    <dbReference type="PhylomeDB" id="Q97XE9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="146" mass="16575" checksum="561B56FD90A693F0" modified="2001-10-01" version="1">MGFNDVFLLMGYTTSKLKSFTAFRKYKGWGRKHGKSYFGFKVCNLVERRTNFVRGFKVGLANLRDLAFSFDNVKLMADRAWIFREDVLVKGVGSAGLPVEGSGVEIREGKASSTTLRGVVMEVFFLNLYRDLEILSTRIRTKVLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XG8</accession>
    <name>Q97XG8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug-efflux transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO1773</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90338">
        <property type="entry name" value="G90338"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XG8"/>
    <dbReference type="PaxDb" id="273057-SSO1773"/>
    <dbReference type="EnsemblBacteria" id="AAK41966">
        <property type="protein sequence ID" value="AAK41966"/>
        <property type="gene ID" value="SSO1773"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1773"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1822"/>
    <dbReference type="eggNOG" id="arCOG00132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_60_4_2"/>
    <dbReference type="InParanoid" id="Q97XG8"/>
    <dbReference type="PhylomeDB" id="Q97XG8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050171">
        <property type="entry name" value="MFS_Transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517:SF14">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517">
        <property type="entry name" value="RESISTANCE PROTEIN MDTM, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="265" mass="29929" checksum="EBACD340D8A4E780" modified="2001-10-01" version="1">MSSPSVKDLLLSFSRFRAGINLGWVIGPIIGGLLYYYLGWNFILLLGSLGNLISIPIVYKLDNRKSVNNVVNRKYFIKPSKYLMEIILVQFIFAFVASQILFGFTVYSTNILKIPSLLIGYFISLNSLLSALFQEPIGRYMIKLNLKYMYILGSFVFSMSYLSIFYLGNIIGLTLFIIFISLGEIILNPIILSISYIVSYKYHSKEIGMAMGFTRMAEGLGRAFSTSVTAYLFSIHDYEVSFTLLSLIGIIGLIVNRVVYTSLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XK6</accession>
    <name>Q97XK6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1727</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90333">
        <property type="entry name" value="G90333"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923684.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XK6"/>
    <dbReference type="STRING" id="273057.SSO1727"/>
    <dbReference type="PaxDb" id="273057-SSO1727"/>
    <dbReference type="EnsemblBacteria" id="AAK41926">
        <property type="protein sequence ID" value="AAK41926"/>
        <property type="gene ID" value="SSO1727"/>
    </dbReference>
    <dbReference type="GeneID" id="1454705"/>
    <dbReference type="KEGG" id="sso:SSO1727"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1770"/>
    <dbReference type="eggNOG" id="arCOG02656">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1500348_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XK6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.30">
        <property type="entry name" value="AF1862-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023101">
        <property type="entry name" value="AF1862-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158568">
        <property type="entry name" value="AF1862-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="179" mass="20687" checksum="B79D92E491F5C18A" modified="2001-10-01" version="1">MSKLDEAVNLAIEFGEKIFRLTEICSSKEFISGFRARAREFPLMVRQMGLIPSLTFLFSKIDDGVLIPWLYYLVKEDVKDTKKICNEVRNEGYTSYLMVNLNSIKRKFYYFRFFDKCIEELGSKSEIPSDFISCVKKDFVSMLNELKSNSKLLAEVEDFVLNFSIELKKIVDGIYGGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XL2</accession>
    <name>Q97XL2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1491</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90332">
        <property type="entry name" value="H90332"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XL2"/>
    <dbReference type="PaxDb" id="273057-SSO8760"/>
    <dbReference type="EnsemblBacteria" id="AAK41919">
        <property type="protein sequence ID" value="AAK41919"/>
        <property type="gene ID" value="SSO8760"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8760"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1763"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180515_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XL2"/>
    <dbReference type="PhylomeDB" id="Q97XL2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="76" mass="8966" checksum="67D288E4D56D431F" modified="2001-10-01" version="1">MESEIKTRGFAIDVSRDKLTVVKAELVITQEKREVEVNEVREFKYDNEGIEELIKFLGEYKEGIMESTGPYFFYLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XQ8</accession>
    <name>Q97XQ8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1652</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90326">
        <property type="entry name" value="B90326"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923548.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XQ8"/>
    <dbReference type="PaxDb" id="273057-SSO1652"/>
    <dbReference type="EnsemblBacteria" id="AAK41865">
        <property type="protein sequence ID" value="AAK41865"/>
        <property type="gene ID" value="SSO1652"/>
    </dbReference>
    <dbReference type="GeneID" id="1454644"/>
    <dbReference type="KEGG" id="sso:SSO1652"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1701"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_2_2"/>
    <dbReference type="InParanoid" id="Q97XQ8"/>
    <dbReference type="PhylomeDB" id="Q97XQ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="175"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="300"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="408" mass="47769" checksum="FE77F5186227AC80" modified="2001-10-01" version="1">MPTLGFRFRAYTDEQTLRALKAQLKLACEVYNTLRWADIYFYQRDGKGLTQTELRQLALDLRKQDDKYKQLYSQVVQQIADRYYEAKKRFFEGLARFPKEKKPHKYYSLVYPQYGWKILQVREIRKGSKKKLITLKLSNLGTFKVIVHRDFPLDKVKRVIVKLTRSERIYITFVVDHEFPKLPNTGKVVAIDVGVEKLLVTSDSEYFPNLRPLEKALWKVKHLHRELSRKKFLSNNWFKAKVKLARAYEYLKNLRSNLYMKLGKWFAEHYDVVVMEGIHVKQLIGKSLRSLRRRLSDVAFSELRDLIKYQLEKYGKKLILVNPAYTSKTCAKCGYVKEDLSLSDRVFVCPNCGWIADRDYNASLNILRGSGSERSLVPVELRPLPVLWHGRAVKQEAPSLGGGSSPYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XS4</accession>
    <name>Q97XS4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6036 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90324">
        <property type="entry name" value="A90324"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923535.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XS4"/>
    <dbReference type="PaxDb" id="273057-SSO1635"/>
    <dbReference type="EnsemblBacteria" id="AAK41848">
        <property type="protein sequence ID" value="AAK41848"/>
        <property type="gene ID" value="SSO1635"/>
    </dbReference>
    <dbReference type="GeneID" id="15297409"/>
    <dbReference type="KEGG" id="sso:SSO1635"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1679"/>
    <dbReference type="eggNOG" id="arCOG05472">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106322_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XS4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045792">
        <property type="entry name" value="DUF6036"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19502">
        <property type="entry name" value="DUF6036"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6036" evidence="1">
        <location>
            <begin position="26"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="186" mass="21662" checksum="42C1E93B7DD947C4" modified="2001-10-01" version="1">MLRELLSRISELKGSETDRFLKFLAILNTLLEDKGLGRIIIVGGFAVELYSGRSYRTGDVDVIVEGIAENLVRQVLKEISDFGLRIYLPKIREISEKGIDIVDNVYNKRKSPLKVQVDSYYIYITPPEEVVLTYLEAWKFWNSIEDRNKAVLVYCAQRNRIDVNYINLESERRGVKDFLERVKDYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XV0</accession>
    <name>Q97XV0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8472</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90320">
        <property type="entry name" value="E90320"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923509.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XV0"/>
    <dbReference type="STRING" id="273057.SSO8472"/>
    <dbReference type="PaxDb" id="273057-SSO8472"/>
    <dbReference type="EnsemblBacteria" id="AAK41820">
        <property type="protein sequence ID" value="AAK41820"/>
        <property type="gene ID" value="SSO8472"/>
    </dbReference>
    <dbReference type="GeneID" id="84057997"/>
    <dbReference type="KEGG" id="sso:SSO8472"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1647"/>
    <dbReference type="eggNOG" id="arCOG07237">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2784283_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XV0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="68" mass="7614" checksum="29C8BFA7E407EAA9" modified="2001-10-01" version="1">MTTFKSVHLALIPQLEERLSKIGDVEVMNEVKKLKSALPNNVVLVTMDKNLASNISLNLKTITLESYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XW7</accession>
    <name>Q97XW7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1593</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="C90318"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    <dbReference type="PaxDb" id="273057-SSO1593"/>
    <dbReference type="EnsemblBacteria" id="AAK41802">
        <property type="protein sequence ID" value="AAK41802"/>
        <property type="gene ID" value="SSO1593"/>
    </dbReference>
    <dbReference type="GeneID" id="7806179"/>
    <dbReference type="KEGG" id="sso:SSO1593"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1627"/>
    <dbReference type="eggNOG" id="arCOG02792">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_055959_0_0_2"/>
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    <dbReference type="PhylomeDB" id="Q97XW7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Major facilitator superfamily MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051084">
        <property type="entry name" value="H+-coupled_symporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43528">
        <property type="entry name" value="ALPHA-KETOGLUTARATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43528:SF1">
        <property type="entry name" value="ALPHA-KETOGLUTARATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0769">Symport</keyword>
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    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="435" mass="48139" checksum="D5583F43EB10F11A" modified="2001-10-01" version="1">MEKSIERLIDTAKWTSIHSLMFASLAIGYFMWGVIASIAPLIYPNINSVLFLLTPTFATLAGNLALSLFSDKKLGRKTTFFITMSLYSVGTILLVLASVLAGFSTDNLAKFPSLILIILGIVLGIFGVEGEVPVMLSYTAEMMPLKYRDMMLVLAPNFDNIGAMVAALVGYLTYSLSNSFIIELLALTVVAILGIVTAVIIRLLLPESVRWLATKGDINKAKIEASKVVKDGTTEAKEVNVNKKLSLGSRYAFLAIIGLSQYLTYGLMAFVVADYYFSSSQTPFIIFIANLGASISGFIAAYIANKIRTRIFALISYVGGSLSMIPILYLTTNFNFGMFYSLLIVNMLFSEFSWAIRTIYEPVLMPKKLRAFMIGLIRLVPITAYAISTYVTQSFNLTNYILYNTILWLIGGIATIIWYFKGIDTNYVSLEEVER</sequence>
</entry>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblBacteria" id="AAK41745">
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    </dbReference>
    <dbReference type="GeneID" id="1454524"/>
    <dbReference type="KEGG" id="sso:SSO1522"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="110" mass="12760" checksum="9D701027B3DCFF56" modified="2001-10-01" version="1">MKVLIKDVDEKLYRMLKAKALIEGISVSEAINEAIKLWLINKDLDRIMVIKSKDFWDAVNEGKYALFCDNNFIGSFENEDEMIKEARKYKKCYALSEKWLVGEGELTGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y41</accession>
    <name>Q97Y41_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">One of two inversely orientated ORFs in ISC1043</fullName>
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        <name evidence="2" type="ordered locus">SSO1508</name>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AAK41730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90309">
        <property type="entry name" value="C90309"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y41"/>
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    <dbReference type="EnsemblBacteria" id="AAK41730">
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        <property type="gene ID" value="SSO1508"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1508"/>
    <dbReference type="HOGENOM" id="CLU_1727318_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y41"/>
    <dbReference type="PhylomeDB" id="Q97Y41"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="151" mass="17209" checksum="FB0C7AB242A103CF" modified="2001-10-01" version="1">MDPLFPDLIYDPPCPPFNEDQSLSRLKYSSKYSSGFFMNFNIISLHLFPPLIVALGYQVLRIREVIQYRLAIVLVSPQGVNLYTKIPQDLEDHLISLPLTLKRITVSDNQVTTQVKDEEGVRELLPSHLPQTSPFHSFNAWLLLGNNLSCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y50</accession>
    <name>Q97Y50_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90308">
        <property type="entry name" value="B90308"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y50"/>
    <dbReference type="PaxDb" id="273057-SSO1496"/>
    <dbReference type="EnsemblBacteria" id="AAK41721">
        <property type="protein sequence ID" value="AAK41721"/>
        <property type="gene ID" value="SSO1496"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1496"/>
    <dbReference type="HOGENOM" id="CLU_2191147_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y50"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="108" mass="11946" checksum="3206931FF22F88EA" modified="2001-10-01" version="1">MGTPRYSTRYSPWGNSPTGTDLPPSLTRALDLSSPTVSFKRALELTYQESKTLHTTLNSFSSIGKVSKYSCILVLNSTSSPLTPQQPFVVYTVLLLLRRPRLPSSLLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2025-06-18" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y88</accession>
    <name>CSA3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR locus-related putative DNA-binding protein Csa3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">csa3</name>
        <name type="ordered locus">SSO1445</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="J. Mol. Biol." volume="405" first="939" last="955">
            <title>The structure of the CRISPR-associated protein Csa3 provides insight into the regulation of the CRISPR/Cas system.</title>
            <authorList>
                <person name="Lintner N.G."/>
                <person name="Frankel K.A."/>
                <person name="Tsutakawa S.E."/>
                <person name="Alsbury D.L."/>
                <person name="Copie V."/>
                <person name="Young M.J."/>
                <person name="Tainer J.A."/>
                <person name="Lawrence C.M."/>
            </authorList>
            <dbReference type="PubMed" id="21093452"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2010.11.019"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR-associated Csa3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90302">
        <property type="entry name" value="G90302"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923410.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2WTE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B=1-237"/>
    </dbReference>
    <dbReference type="PDB" id="6W11">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.46 A"/>
        <property type="chains" value="A/B=1-212"/>
    </dbReference>
    <dbReference type="PDB" id="6WXQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.05 A"/>
        <property type="chains" value="A/B=1-237"/>
    </dbReference>
    <dbReference type="PDBsum" id="2WTE"/>
    <dbReference type="PDBsum" id="6W11"/>
    <dbReference type="PDBsum" id="6WXQ"/>
    <dbReference type="AlphaFoldDB" id="Q97Y88"/>
    <dbReference type="SMR" id="Q97Y88"/>
    <dbReference type="STRING" id="273057.SSO1445"/>
    <dbReference type="PaxDb" id="273057-SSO1445"/>
    <dbReference type="DNASU" id="1454457"/>
    <dbReference type="EnsemblBacteria" id="AAK41678">
        <property type="protein sequence ID" value="AAK41678"/>
        <property type="gene ID" value="SSO1445"/>
    </dbReference>
    <dbReference type="GeneID" id="1454457"/>
    <dbReference type="GeneID" id="27427813"/>
    <dbReference type="KEGG" id="sso:SSO1445"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1476"/>
    <dbReference type="eggNOG" id="arCOG01446">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107894_1_0_2"/>
    <dbReference type="InParanoid" id="Q97Y88"/>
    <dbReference type="EvolutionaryTrace" id="Q97Y88"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09655">
        <property type="entry name" value="CasRa_I-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010163">
        <property type="entry name" value="Csa3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054588">
        <property type="entry name" value="Csa3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01884">
        <property type="entry name" value="cas_HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22662">
        <property type="entry name" value="Csa3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25212">
        <property type="entry name" value="HVO_A0114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000417889" description="CRISPR locus-related putative DNA-binding protein Csa3">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="3"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="13"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="61"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="87"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="94"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="115"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="128"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="133"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="162"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="188"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="193"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="197"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="202"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="21093452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2WTE"/>
        </source>
    </evidence>
    <sequence length="237" mass="26816" checksum="BC4DC274587E7CE2" modified="2001-10-01" version="1">MKSYFVTMGFNETFLLRLLNETSAQKEDSLVIVVPSPIVSGTRAAIESLRAQISRLNYPPPRIYEIEITDFNLALSKILDIILTLPEPIISDLTMGMRMINTLILLGIIVSRKRFTVYVRDEGGGSRVISFNDNTIRALMRDYSREEMKLLNVLYETKGTGITELAKMLDKSEKTLINKIAELKKFGILTQKGKDRKVELNELGLNVIKLNKSVIESSKSSEELVKENKGKEVNIPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YH1</accession>
    <name>Q97YH1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative peptidoglycan binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90291">
        <property type="entry name" value="C90291"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923343.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YH1"/>
    <dbReference type="STRING" id="273057.SSO1350"/>
    <dbReference type="PaxDb" id="273057-SSO1350"/>
    <dbReference type="EnsemblBacteria" id="AAK41586">
        <property type="protein sequence ID" value="AAK41586"/>
        <property type="gene ID" value="SSO1350"/>
    </dbReference>
    <dbReference type="GeneID" id="1454365"/>
    <dbReference type="KEGG" id="sso:SSO1350"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1356"/>
    <dbReference type="eggNOG" id="arCOG04204">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068244_0_1_2"/>
    <dbReference type="InParanoid" id="Q97YH1"/>
    <dbReference type="PhylomeDB" id="Q97YH1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010430">
        <property type="entry name" value="DUF1028"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014927">
        <property type="entry name" value="PG-bd_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39328">
        <property type="entry name" value="BLL2871 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39328:SF1">
        <property type="entry name" value="BLL2871 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06267">
        <property type="entry name" value="DUF1028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08823">
        <property type="entry name" value="PG_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative peptidoglycan binding" evidence="1">
        <location>
            <begin position="210"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="268" mass="29577" checksum="0F5F92F1A2C60EF7" modified="2001-10-01" version="1">MTFSIVIYDPDEKAWGVGVASKFLAVGAFVPWLKPNVGAIATQALANLTYGINGLSLLEKGYSASDSIRILTESDPLREKRQIGIVDSKGNASAFTGRECYSYAGHIVGNNFTVQGNILAGEEVLEAMAKEAEGKGKIYEKILRALKAGESKGGDRRGKQSAAIIVVRAVERSEREIDPLVVGKYVDLRVDDSPDPLRDLERLLDLWVATFVEEEMVNVKDYEDQIRDALKRWGYSDLRTWVEINNFEGKYTGDKIGKSVLKILLSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YM0</accession>
    <name>Q97YM0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO7635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90285">
        <property type="entry name" value="D90285"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YM0"/>
    <dbReference type="PaxDb" id="273057-SSO7635"/>
    <dbReference type="EnsemblBacteria" id="AAK41539">
        <property type="protein sequence ID" value="AAK41539"/>
        <property type="gene ID" value="SSO7635"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7635"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1303"/>
    <dbReference type="eggNOG" id="arCOG09924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2519932_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YM0"/>
    <dbReference type="PhylomeDB" id="Q97YM0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="84" mass="9465" checksum="F1BC6A83CDE3173F" modified="2001-10-01" version="1">MQTIQVSITELKSLAINLATININLISQDLDPEIVKAAPSLLTGNRGKYYLKVVRQGEKVISKGQRTFKFYPIYREVKGEINVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YN2</accession>
    <name>Q97YN2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">XdhC Rossmann domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90284">
        <property type="entry name" value="C90284"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991989.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YN2"/>
    <dbReference type="SMR" id="Q97YN2"/>
    <dbReference type="STRING" id="273057.SSO1292"/>
    <dbReference type="PaxDb" id="273057-SSO1292"/>
    <dbReference type="EnsemblBacteria" id="AAK41530">
        <property type="protein sequence ID" value="AAK41530"/>
        <property type="gene ID" value="SSO1292"/>
    </dbReference>
    <dbReference type="GeneID" id="1454309"/>
    <dbReference type="KEGG" id="sso:SSO1292"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1291"/>
    <dbReference type="eggNOG" id="arCOG01929">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1418746_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YN2"/>
    <dbReference type="PhylomeDB" id="Q97YN2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052698">
        <property type="entry name" value="MoCofactor_Util/Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027051">
        <property type="entry name" value="XdhC_Rossmann_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30388">
        <property type="entry name" value="ALDEHYDE OXIDOREDUCTASE MOLYBDENUM COFACTOR ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30388:SF6">
        <property type="entry name" value="XANTHINE DEHYDROGENASE SUBUNIT A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13478">
        <property type="entry name" value="XdhC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XdhC Rossmann" evidence="1">
        <location>
            <begin position="38"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="191" mass="21295" checksum="02B053A6A048B4F1" modified="2001-10-01" version="1">MKVVIFASSREADMEEPVFCDRDTVKVDASKCTGKGINVAPFIARYLKDLGFHVLVVDPFAEESYYEADEVIKGTTFQIPDDVVKDNYVIVATRHVYDTWAIMKSIYGGAKEIAVIMSLKRAEVILKRLIQAGIDKEKLKKLRIPAGLDIGAKNEKEIAFSIVAEVLAVTRGSMGLPLREIKGFEKLLEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YN3</accession>
    <name>Q97YN3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1291</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99284">
        <property type="entry name" value="B99284"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YN3"/>
    <dbReference type="STRING" id="273057.SSO1291"/>
    <dbReference type="PaxDb" id="273057-SSO1291"/>
    <dbReference type="EnsemblBacteria" id="AAK41529">
        <property type="protein sequence ID" value="AAK41529"/>
        <property type="gene ID" value="SSO1291"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1291"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1290"/>
    <dbReference type="eggNOG" id="arCOG03654">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031607_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YN3"/>
    <dbReference type="PhylomeDB" id="Q97YN3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="446" mass="48710" checksum="1EF8378F4625AB50" modified="2001-10-01" version="1">MFYCMKEEIDKKEEMKTSKKLTFKDVFFISFGGQAPFISLLTFGTVMIGKVGTQAPFAMLIATLVVLFNGLVIYSLSGRFKRGGGYYTYAFYSLTSRLGLNTGWNYLLYGLAYGGTLLTGGAYVLYTIISTYIPSTMLPSIFYDQWFYALIIGIIATSIVLAGVQVSAKYAIGASIVEILIISLLSLLFLYDSRWNFYNPIPSSITPTLISAVVFGLGIPTGYGSIAPLGGETNPKTIGKAAISVLLFGGLIATFFFYSLAAMNFTGNLIDYLLFRFGLIGTLVIALIALSDGTLGGMTYILANSRTLKAMAEDKIMPSGLSKVKYSELLVGLVFITSLTAMTYTFGLYNVFTILGALAGLNNLFIHISANSSLVRIASKRALKHLHEVFIGIFASLVSISVFLYSLPTFNKYIVYLFFGWIILGFIYAEALEISRQSSESENSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YS7</accession>
    <name>Q97YS7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Toluene-4-monooxygenase system protein D. (TmoD)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tmoD</name>
        <name evidence="1" type="ordered locus">SSO1231</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90277">
        <property type="entry name" value="F90277"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992603.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YS7"/>
    <dbReference type="FunCoup" id="Q97YS7">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1231"/>
    <dbReference type="PaxDb" id="273057-SSO1231"/>
    <dbReference type="EnsemblBacteria" id="AAK41477">
        <property type="protein sequence ID" value="AAK41477"/>
        <property type="gene ID" value="SSO1231"/>
    </dbReference>
    <dbReference type="GeneID" id="1454256"/>
    <dbReference type="KEGG" id="sso:SSO1231"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1231"/>
    <dbReference type="eggNOG" id="arCOG08362">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148539_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YS7"/>
    <dbReference type="PhylomeDB" id="Q97YS7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.56.10">
        <property type="entry name" value="Monooxygenase component MmoB/DmpM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003454">
        <property type="entry name" value="MOase_MmoB_DmpM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036889">
        <property type="entry name" value="mOase_MmoB_DmpM_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02406">
        <property type="entry name" value="MmoB_DmpM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56029">
        <property type="entry name" value="Monooxygenase (hydroxylase) regulatory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="115" mass="13105" checksum="E9B4E269202BEB35" modified="2001-10-01" version="1">MSLELTLETILDKYQIMDLSKLPQNMVGPVLMKDEFSYAVVEALARDNPNTFKGVLDRGSYIRVVGERELILNKTTLEEVIGMDVRFPGEVEVRMSAFAGKIIVRGDYIKWHLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YU8</accession>
    <name>Q97YU8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pyruvate synthase delta chain (Pyruvic-ferredoxin oxidoreductase delta chain) (PorD-1)</fullName>
            <ecNumber evidence="9">1.2.7.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">porD-1</name>
        <name evidence="9" type="ordered locus">SSO7412</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.2.7.1" evidence="9"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90274">
        <property type="entry name" value="H90274"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990062.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YU8"/>
    <dbReference type="FunCoup" id="Q97YU8">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO7412"/>
    <dbReference type="PaxDb" id="273057-SSO7412"/>
    <dbReference type="EnsemblBacteria" id="AAK41455">
        <property type="protein sequence ID" value="AAK41455"/>
        <property type="gene ID" value="SSO7412"/>
    </dbReference>
    <dbReference type="GeneID" id="1454234"/>
    <dbReference type="KEGG" id="sso:SSO7412"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1206"/>
    <dbReference type="eggNOG" id="arCOG01605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_1_1_2"/>
    <dbReference type="InParanoid" id="Q97YU8"/>
    <dbReference type="PhylomeDB" id="Q97YU8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011898">
        <property type="entry name" value="PorD_KorD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02179">
        <property type="entry name" value="PorD_KorD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724:SF1">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="27"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="57"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="88" mass="9822" checksum="5199856A61E70B3C" modified="2001-10-01" version="1">MIKVPIGVPVARPKIGSAGKTGLWRVEKPVISYDKCTKCRLCVLYCLENTIDLLENLYPQIDYDYCKGCGVCAQVCPPKAIDMVREVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YX3</accession>
    <name>Q97YX3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO7348</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90271">
        <property type="entry name" value="D90271"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YX3"/>
    <dbReference type="STRING" id="273057.SSO7348"/>
    <dbReference type="PaxDb" id="273057-SSO7348"/>
    <dbReference type="EnsemblBacteria" id="AAK41427">
        <property type="protein sequence ID" value="AAK41427"/>
        <property type="gene ID" value="SSO7348"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7348"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1175"/>
    <dbReference type="eggNOG" id="arCOG02689">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2447810_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YX3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="89" mass="9503" checksum="FBE630E827CB3584" modified="2001-10-01" version="1">MLGVELAPTKIRGIAQSITVAAGRIGAALTAFVFPSFFALYGESFAITFLAIVAGISSVITFLFVPETKGKPLEESSREVSIMEKYATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YZ9</accession>
    <name>Q97YZ9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lrp/AsnC family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90268">
        <property type="entry name" value="B90268"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YZ9"/>
    <dbReference type="STRING" id="273057.SSO1150"/>
    <dbReference type="PaxDb" id="273057-SSO1150"/>
    <dbReference type="EnsemblBacteria" id="AAK41401">
        <property type="protein sequence ID" value="AAK41401"/>
        <property type="gene ID" value="SSO1150"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1150"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1144"/>
    <dbReference type="eggNOG" id="arCOG08454">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066382_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YZ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="41807" checksum="28456D46C63AA99C" modified="2001-10-01" version="1">MSMIDSTVIQKLTKSLHYVQRFGDHNHELLGELTGLENVRNVLSNLKVLVIPSPKLDSLGLSLFHTTIKLTRNSKVQPLDFYYLLDGMVVASLRDVLTSDTIVLDVVFPRGRWDFVQILEMMSRGGLIDNYKTYLEYRREVYPVDYSTFNFDTLTFERVILDKPREPFQLPDLTDDFKPDWIDVYTLGKKQENPLRSWDEIAESIGLTSDEVVDHYFNHVVGKGLVDAFYTYLGKTDFRMSLIVNDTNEEITRELSRIVTLLRISYLHNDKIYAFIVGQRHTLIDIMEFINKLSSAYHFSYEIYVHPITPLRDFVRTYSIPYEHFTKDNKWSITLKELKENFEKELSRVLEERKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z11</accession>
    <name>Q97Z11_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Heterodisulfide reductase, subunit B (HdrB-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hdrB-2</name>
        <name evidence="3" type="ordered locus">SSO1135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90266">
        <property type="entry name" value="D90266"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989762.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z11"/>
    <dbReference type="FunCoup" id="Q97Z11">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1135"/>
    <dbReference type="PaxDb" id="273057-SSO1135"/>
    <dbReference type="EnsemblBacteria" id="AAK41387">
        <property type="protein sequence ID" value="AAK41387"/>
        <property type="gene ID" value="SSO1135"/>
    </dbReference>
    <dbReference type="GeneID" id="1454166"/>
    <dbReference type="KEGG" id="sso:SSO1135"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1127"/>
    <dbReference type="eggNOG" id="arCOG00338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052147_1_0_2"/>
    <dbReference type="InParanoid" id="Q97Z11"/>
    <dbReference type="PhylomeDB" id="Q97Z11"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051278">
        <property type="entry name" value="HdrB/HdrD_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42947">
        <property type="entry name" value="COB--COM HETERODISULFIDE REDUCTASE SUBUNIT B 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42947:SF1">
        <property type="entry name" value="COB--COM HETERODISULFIDE REDUCTASE SUBUNIT B 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cysteine-rich" evidence="2">
        <location>
            <begin position="10"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Cysteine-rich" evidence="2">
        <location>
            <begin position="159"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="305" mass="33992" checksum="9998F1EC5DD62F9E" modified="2001-10-01" version="1">MTLPTPYGKVAFYPGCALDGLGKSYDVSLKLVAQDLGIPLEKIEDYNCCGALEVKNVNTMAGVLLPARNLALAREMGADAVVSACPGCHYSLSRTQYYLTKYPKLREKTNMYLEKMGTKNYDLKLMLVHAVEYIYNTVGIEAIKTKVRRPLTGLKVAPYYGCLYVRPKSYTLAGYMKMRDDPERPFFMDEILKALGAEVVPFEAKTMCCGGPHVYSDVEVALHLEARILKEAKRNGAEILVTDCPLGHVAIETNMEKIAQKYGEDLRTPLAYFSQLVAFAFGHSPEETLLTANITNPMSILKRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z40</accession>
    <name>Q97Z40_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2258 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90262">
        <property type="entry name" value="E90262"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z40"/>
    <dbReference type="STRING" id="273057.SSO1098"/>
    <dbReference type="PaxDb" id="273057-SSO1098"/>
    <dbReference type="EnsemblBacteria" id="AAK41356">
        <property type="protein sequence ID" value="AAK41356"/>
        <property type="gene ID" value="SSO1098"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1098"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1094"/>
    <dbReference type="eggNOG" id="arCOG03772">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691618_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z40"/>
    <dbReference type="PhylomeDB" id="Q97Z40"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017140">
        <property type="entry name" value="ThermoDBP-RPs_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10015">
        <property type="entry name" value="ThermoDBP-RP_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037214">
        <property type="entry name" value="UCP037214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="18157" checksum="D9C25620A8779217" modified="2001-10-01" version="1">MMSEEINRDMERAEEYEQTTTRVSVLGQNRFELSTGLIIAARYADKLRRVALVAFSKIAPKEVIIRDVSELNKQLYTKIVEEMKLGKLDVIRISVDAEYDDRNKKLIFSNLRILRYITEEQCGEKYKDIISENERLKGEILELKKKLEDILSLLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z86</accession>
    <name>KPRS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribose-phosphate pyrophosphokinase</fullName>
            <shortName evidence="1">RPPK</shortName>
            <ecNumber evidence="1">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1 3">5-phospho-D-ribosyl alpha-1-diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosyl pyrophosphate synthase</fullName>
            <shortName evidence="1">P-Rib-PP synthase</shortName>
            <shortName evidence="1 3">PRPP synthase</shortName>
            <shortName evidence="1">PRPPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 3" type="primary">prs</name>
        <name type="ordered locus">SSO1045</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2017" name="Microbiol. Mol. Biol. Rev." volume="81" first="E00040" last="E00040">
            <title>Phosphoribosyl diphosphate (PRPP): biosynthesis, enzymology, utilization, and metabolic significance.</title>
            <authorList>
                <person name="Hove-Jensen B."/>
                <person name="Andersen K.R."/>
                <person name="Kilstrup M."/>
                <person name="Martinussen J."/>
                <person name="Switzer R.L."/>
                <person name="Willemoes M."/>
            </authorList>
            <dbReference type="PubMed" id="28031352"/>
            <dbReference type="DOI" id="10.1128/MMBR.00040-16"/>
        </citation>
        <scope>REVIEW</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2015" name="Extremophiles" volume="19" first="407" last="415">
            <title>Structure of dimeric, recombinant Sulfolobus solfataricus phosphoribosyl diphosphate synthase: a bent dimer defining the adenine specificity of the substrate ATP.</title>
            <authorList>
                <person name="Andersen R.W."/>
                <person name="Leggio L.L."/>
                <person name="Hove-Jensen B."/>
                <person name="Kadziola A."/>
            </authorList>
            <dbReference type="PubMed" id="25605536"/>
            <dbReference type="DOI" id="10.1007/s00792-014-0726-x"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) IN COMPLEX WITH ATP ANALOG</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 6">Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the ribose-phosphate pyrophosphokinase family. Class III (archaeal) subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK41307" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="1"/>
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        <property type="protein sequence ID" value="AAK41307.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="D90256"/>
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    <dbReference type="RefSeq" id="WP_009989183.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="4TWB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-291"/>
    </dbReference>
    <dbReference type="PDBsum" id="4TWB"/>
    <dbReference type="AlphaFoldDB" id="Q97Z86"/>
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    <dbReference type="STRING" id="273057.SSO1045"/>
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    <dbReference type="EnsemblBacteria" id="AAK41307">
        <property type="protein sequence ID" value="AAK41307"/>
        <property type="gene ID" value="SSO1045"/>
    </dbReference>
    <dbReference type="GeneID" id="1454086"/>
    <dbReference type="GeneID" id="44129974"/>
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    <dbReference type="PATRIC" id="fig|273057.12.peg.1039"/>
    <dbReference type="eggNOG" id="arCOG00067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033546_2_2_2"/>
    <dbReference type="InParanoid" id="Q97Z86"/>
    <dbReference type="PhylomeDB" id="Q97Z86"/>
    <dbReference type="BRENDA" id="2.7.6.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97Z86"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000074">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000083">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_A">
        <property type="entry name" value="RibP_PPkinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037514">
        <property type="entry name" value="Rib-P_diPkinase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF32">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000141248" description="Ribose-phosphate pyrophosphokinase">
        <location>
            <begin position="1"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 6 7">
        <location>
            <begin position="34"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 6 7">
        <location>
            <begin position="93"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2 7">
        <location>
            <begin position="220"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="2"/>
            <end position="5"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="12"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
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            <begin position="24"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
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            <begin position="28"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
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            <end position="40"/>
        </location>
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    <feature type="strand" evidence="8">
        <location>
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        </location>
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        </location>
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        <location>
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            <end position="114"/>
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            <begin position="115"/>
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        </location>
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        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
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            <begin position="157"/>
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        </location>
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        </location>
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        <location>
            <begin position="284"/>
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        </location>
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    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="25605536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="25605536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000305" key="6">
        <source>
            <dbReference type="PubMed" id="25605536"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
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        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="4TWB"/>
        </source>
    </evidence>
    <sequence length="291" mass="32179" checksum="A18743D629CF60A6" modified="2002-10-25" version="2">MIIIGGSATNGIDESLSKILSIPLVKVENKIFPDGESYIRVPSSIRDEEVLLVQTTDYPQDKHLIELFLIAETIRDLGAKKLTAIVPYLAYSRQDRRFKDGEAISIKTILHILSEVGVNTLVVVEPHKPEELSYFKGELKIVHPYHQIARKIKEIIEDPFILAPDRGALDRARKIAEEINAPYSYIEKERDRTTGEVRIKEAPNINLKGKDVVIIDDIISTGGTIVQATRLAYSLGAKSVTAAAIHLLLVGGAKERLREVGVKTLIGTNTINVNDKDIITIDVSQSIALSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZG4</accession>
    <name>Q97ZG4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF357 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO0954</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90246">
        <property type="entry name" value="E90246"/>
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    <dbReference type="RefSeq" id="WP_009992393.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="PATRIC" id="fig|273057.12.peg.950"/>
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    </dbReference>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_1212640_0_0_2"/>
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        <property type="component" value="Chromosome"/>
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        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
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        <property type="entry name" value="cytidylyltransferase"/>
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        <property type="entry name" value="AF1782-like"/>
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        <property type="entry name" value="HUPs"/>
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        <property type="entry name" value="AF1782-like_sf"/>
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        <property type="entry name" value="Archaeal_FAD_synthase"/>
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    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023140">
        <property type="entry name" value="DUF357"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43793">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43793:SF1">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04010">
        <property type="entry name" value="DUF357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158372">
        <property type="entry name" value="AF1782-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DUF357" evidence="4">
        <location>
            <begin position="12"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Cytidyltransferase-like" evidence="3">
        <location>
            <begin position="86"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="26289" checksum="CF5CFD38B0BC0CE6" modified="2001-10-01" version="1">MDIEELKKRIEKYIKGMDERLKDTSSANNKVVELARLYTEDAKYYLEKGDYITALVDVVYAEGLLDAKEIYENSDPKSNVSKKVFVAGTFDILHPGHIEFLKEASKYGRVYVTVARDSNSERIKGRKPINDEQTRLEIIKSIRYVFDAILGDQEDFLKSVERINPDIIFLGPDQKVDEAKLLEELKKRGLSPQIIRLNERIRKWSHSSTTDIINEIKKRYCNLEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZK5</accession>
    <name>Q97ZK5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0902</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90240">
        <property type="entry name" value="H90240"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992324.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZK5"/>
    <dbReference type="STRING" id="273057.SSO0902"/>
    <dbReference type="PaxDb" id="273057-SSO0902"/>
    <dbReference type="EnsemblBacteria" id="AAK41183">
        <property type="protein sequence ID" value="AAK41183"/>
        <property type="gene ID" value="SSO0902"/>
    </dbReference>
    <dbReference type="GeneID" id="1455150"/>
    <dbReference type="KEGG" id="sso:SSO0902"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.903"/>
    <dbReference type="eggNOG" id="arCOG08333">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2243970_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZK5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="104" mass="12223" checksum="435D5035766C068E" modified="2001-10-01" version="1">MIVTNYNINGLNFSVIYENERVIIYMDVNKEIKVNKRAKDEERVIYMDVNKEIKNGILRKLIICNTKISSYICNAIVEITTKNIYEDLLLNLYNEVLEVSEMVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-11-27" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZM9</accession>
    <name>Q97ZM9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0858</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90236">
        <property type="entry name" value="G90236"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992280.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZM9"/>
    <dbReference type="STRING" id="273057.SSO0858"/>
    <dbReference type="PaxDb" id="273057-SSO0858"/>
    <dbReference type="EnsemblBacteria" id="AAK41150">
        <property type="protein sequence ID" value="AAK41150"/>
        <property type="gene ID" value="SSO0858"/>
    </dbReference>
    <dbReference type="GeneID" id="71027421"/>
    <dbReference type="KEGG" id="sso:SSO0858"/>
    <dbReference type="eggNOG" id="arCOG06043">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161775_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZM9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="130" mass="15141" checksum="092CB387D3263D26" modified="2001-10-01" version="1">MQKHNVCPYYKNGYCTSPALDKPSDIVTSSNRCFGQFKTCRYFLNDGSDSKIGLDKFNEEKTIEQEIRFYPKINELENIVDSGCEHYRLIKSEKGFIAYCNAIKRVLVTRQTLLCNKEFQRCPYRTLLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZP0</accession>
    <name>Q97ZP0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO6663</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90235">
        <property type="entry name" value="C90235"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923133.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZP0"/>
    <dbReference type="STRING" id="273057.SSO6663"/>
    <dbReference type="PaxDb" id="273057-SSO6663"/>
    <dbReference type="EnsemblBacteria" id="AAK41138">
        <property type="protein sequence ID" value="AAK41138"/>
        <property type="gene ID" value="SSO6663"/>
    </dbReference>
    <dbReference type="GeneID" id="63017551"/>
    <dbReference type="KEGG" id="sso:SSO6663"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.859"/>
    <dbReference type="eggNOG" id="arCOG02227">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3075539_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZP0"/>
    <dbReference type="PhylomeDB" id="Q97ZP0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="52" mass="6027" checksum="4AB90ADC74D23C2A" modified="2001-10-01" version="1">MVKLRKIGEPVNAVDIILSSIALNRDMIIVTNDNDFESIKKVEERLKIEKMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZT0</accession>
    <name>Q97ZT0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0499</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90195">
        <property type="entry name" value="C90195"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991388.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZT0"/>
    <dbReference type="PaxDb" id="273057-SSO0499"/>
    <dbReference type="EnsemblBacteria" id="AAK40818">
        <property type="protein sequence ID" value="AAK40818"/>
        <property type="gene ID" value="SSO0499"/>
    </dbReference>
    <dbReference type="GeneID" id="1455628"/>
    <dbReference type="KEGG" id="sso:SSO0499"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.491"/>
    <dbReference type="eggNOG" id="arCOG05946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_654919_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZT0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <source>
            <dbReference type="EMBL" id="AAK40818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="420" mass="49186" checksum="CCAAD64A4FCD0A7E" modified="2001-10-01" version="1">MKEESYRLLEYVVEHGLEGTLTALETNKGIPIVLVKEDPHTLTTILCIDGIARRITKRFTRTTVHKAIYELIDEIESMISQPIEELRISQKVSFENCIEERGEEKPKRKKRETPRLPSIDEYKRIEIPQKHVIPLLYLGDRKYLSLILELGIIDIIESLSSSPIIIENNQVTPYKIRDMRAVYNVLSLFKLDRFNNSNPFSTISLNRKFLTFFTALYNDVEVLGQTSISMLQRNLKLVKHRVKMFSASKKGNLHTEEVEILNNKNSLERNDIRVGLFLRSNDGNTVQIGDINLGELHEKNVFTVNEYIYSSLYMMEDDDYLFFDNILMKLLNTYIAKSNYSKLTRDIIERETNINYSIPIVMRTMANRIELANPILYWYSKEILNSDEICINCPIIEYVNKFNEFLNNYVRLGYFRSVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZT2</accession>
    <name>Q97ZT2_SACS2</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name evidence="2" type="ordered locus">SSO0496</name>
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        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
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        <dbReference type="NCBI Taxonomy" id="273057"/>
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    </organism>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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                <person name="Van der Oost J."/>
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            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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    </dbReference>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_384809_0_0_2"/>
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        <property type="component" value="Chromosome"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="60"/>
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    </feature>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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    </feature>
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    </feature>
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            <end position="377"/>
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        <location>
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            <begin position="449"/>
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            <begin position="695"/>
            <end position="712"/>
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    </feature>
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            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="EMBL" id="AAK40816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="718" mass="81097" checksum="02901C30EB9942C6" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZU1</accession>
    <name>Q97ZU1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Iron (III) ABC transporter, permease protein (HEMU 1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO0486</name>
    </gene>
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        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="AAK40807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="H90193"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZU1"/>
    <dbReference type="FunCoup" id="Q97ZU1">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0486"/>
    <dbReference type="PaxDb" id="273057-SSO0486"/>
    <dbReference type="EnsemblBacteria" id="AAK40807">
        <property type="protein sequence ID" value="AAK40807"/>
        <property type="gene ID" value="SSO0486"/>
    </dbReference>
    <dbReference type="GeneID" id="15298020"/>
    <dbReference type="KEGG" id="sso:SSO0486"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.480"/>
    <dbReference type="eggNOG" id="arCOG01007">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_0_2_2"/>
    <dbReference type="InParanoid" id="Q97ZU1"/>
    <dbReference type="PhylomeDB" id="Q97ZU1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06550">
        <property type="entry name" value="TM_ABC_iron-siderophores_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472:SF25">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN MJ0876-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="FERRIC ENTEROBACTIN TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
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    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <end position="102"/>
        </location>
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        </location>
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    </feature>
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            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK40807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="328" mass="35682" checksum="C7F720A3D0ABDE0C" modified="2001-10-01" version="1">MVRLSGIFLIIGFIISFLLGLLYGDVTIPISEIFHPSGVYAYILLNIRLPTLTVAILIGAILSTSGAVLQMLLRNPLVDPYISGTASGGAFGAVLTYLLLMLNLPFSWIIYFQPLVAFFTATLATLITIMIGKKSGYLGLVIGGVLVSFIFSSLVTVILSVMSAKYPQIPPLTFWLLGDISVVGWFNAIILSLFAILLLYFSLSNSRLIDLLSISDEISYSQGVNPNKQRVFWLLFISLVVAYCVSIAGIIGFLGIIVPHIIRRLIGGNTSQLVMYSSLLGSTILILSNILSHGIFGYFIPLTALLSIMGAPIMIFALVRKDVNVEEY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZY6</accession>
    <name>RS24_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Small ribosomal subunit protein eS24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">30S ribosomal protein S24e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 6" type="primary">rps24e</name>
        <name type="ordered locus">SSO0435</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7 8 9" key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the 30S small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS24 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="A99188"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
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    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
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    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
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    <dbReference type="PDB" id="9FS6">
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        <property type="resolution" value="2.90 A"/>
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    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
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        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="V=1-120"/>
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    <dbReference type="PDBsum" id="9FHL"/>
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    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q97ZY6"/>
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    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q97ZY6"/>
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        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0435"/>
    <dbReference type="PaxDb" id="273057-SSO0435"/>
    <dbReference type="EnsemblBacteria" id="AAK40760">
        <property type="protein sequence ID" value="AAK40760"/>
        <property type="gene ID" value="SSO0435"/>
    </dbReference>
    <dbReference type="GeneID" id="1455570"/>
    <dbReference type="KEGG" id="sso:SSO0435"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.428"/>
    <dbReference type="eggNOG" id="arCOG04182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107248_3_2_2"/>
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    <dbReference type="PhylomeDB" id="Q97ZY6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00545">
        <property type="entry name" value="Ribosomal_eS24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053709">
        <property type="entry name" value="eRP_eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_eS24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018098">
        <property type="entry name" value="Ribosomal_eS24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10496">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
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    <dbReference type="PROSITE" id="PS00529">
        <property type="entry name" value="RIBOSOMAL_S24E"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000137656" description="Small ribosomal subunit protein eS24">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="120" mass="13097" checksum="613DAAEEF56CB1B4" modified="2001-10-01" version="1">MESQAKVKISDKAEGIIERDMQNSVIGRREISLKVYHMGSGTPSRKDIIKAIIQALGSQENLVVVRKISTSYGAGISNVKLHIYKSREILEKVEPKYLLDRDAGTKQKKGGSKGGQGAKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-30" modified="2026-01-28" version="137" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Q4</accession>
    <name>UPP_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Uracil phosphoribosyltransferase</fullName>
            <ecNumber>2.4.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>UMP pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>UPRTase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">upp</name>
        <name type="ordered locus">SSO0231</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="FEBS J." volume="272" first="1440" last="1453">
            <title>Allosteric properties of the GTP activated and CTP inhibited uracil phosphoribosyltransferase from the thermoacidophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Jensen K.F."/>
                <person name="Arent S."/>
                <person name="Larsen S."/>
                <person name="Schack L."/>
            </authorList>
            <dbReference type="PubMed" id="15752360"/>
            <dbReference type="DOI" id="10.1111/j.1742-4658.2005.04576.x"/>
        </citation>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>FUNCTION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>KINETIC PARAMETERS</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>REACTION MECHANISM</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2005" name="Biochemistry" volume="44" first="883" last="892">
            <title>Allosteric regulation and communication between subunits in uracil phosphoribosyltransferase from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Arent S."/>
                <person name="Harris P."/>
                <person name="Jensen K.F."/>
                <person name="Larsen S."/>
            </authorList>
            <dbReference type="PubMed" id="15654744"/>
            <dbReference type="DOI" id="10.1021/bi048041l"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEXES WITH UMP AND CTP</scope>
        <scope>REACTION MECHANISM</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2009" name="J. Mol. Biol." volume="393" first="464" last="477">
            <title>Structural and kinetic studies of the allosteric transition in Sulfolobus solfataricus uracil phosphoribosyltransferase: Permanent activation by engineering of the C-terminus.</title>
            <authorList>
                <person name="Christoffersen S."/>
                <person name="Kadziola A."/>
                <person name="Johansson E."/>
                <person name="Rasmussen M."/>
                <person name="Willemoes M."/>
                <person name="Jensen K.F."/>
            </authorList>
            <dbReference type="PubMed" id="19683539"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2009.08.019"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEXES WITH GTP; 5-PHOSPHO-ALPHA-D-RIBOSE 1-DIPHOSPHATE; RIBOSE-5-PHOSPHATE AND DIPHOSPHATE</scope>
        <scope>REACTION MECHANISM</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>UMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:13017"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit. The magnesium is bound as Mg-PRPP.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Allosterically activated by GTP. Inhibited by CTP and UMP in combination.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">55 uM for 5-phospho-alpha-D-ribose 1-diphosphate (in the presence of 1 mM GTP)</KM>
            <KM evidence="2">19 uM for 5-phospho-alpha-D-ribose 1-diphosphate (without GTP)</KM>
            <KM evidence="2">27 uM for uracil (in the presence of 1 mM GTP)</KM>
            <KM evidence="2">1.4 uM for uracil (without GTP)</KM>
            <Vmax evidence="2">15.8 umol/min/mg enzyme (in the presence of 1 mM GTP)</Vmax>
            <Vmax evidence="2">0.92 umol/min/mg enzyme (without GTP)</Vmax>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 5.0-5.6 at 60 degrees Celsius.</text>
        </phDependence>
    </comment>
    <comment type="pathway">
        <text>Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90164">
        <property type="entry name" value="G90164"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990487.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1VST">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A=1-216"/>
    </dbReference>
    <dbReference type="PDB" id="1XTT">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B/C/D=1-216"/>
    </dbReference>
    <dbReference type="PDB" id="1XTU">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H=1-216"/>
    </dbReference>
    <dbReference type="PDB" id="1XTV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H=1-216"/>
    </dbReference>
    <dbReference type="PDB" id="3G6W">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="A/B/C/D=1-216"/>
    </dbReference>
    <dbReference type="PDBsum" id="1VST"/>
    <dbReference type="PDBsum" id="1XTT"/>
    <dbReference type="PDBsum" id="1XTU"/>
    <dbReference type="PDBsum" id="1XTV"/>
    <dbReference type="PDBsum" id="3G6W"/>
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        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0231"/>
    <dbReference type="PaxDb" id="273057-SSO0231"/>
    <dbReference type="EnsemblBacteria" id="AAK40574">
        <property type="protein sequence ID" value="AAK40574"/>
        <property type="gene ID" value="SSO0231"/>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_067096_2_0_2"/>
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    <dbReference type="BRENDA" id="2.4.2.9">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00636"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q980Q4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032263">
        <property type="term" value="P:GMP salvage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006178">
        <property type="term" value="P:guanine salvage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046100">
        <property type="term" value="P:hypoxanthine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <keyword id="KW-0021">Allosteric enzyme</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000120927" description="Uracil phosphoribosyltransferase">
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        <location>
            <begin position="210"/>
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    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15752360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="1XTT"/>
        </source>
    </evidence>
    <sequence length="216" mass="24150" checksum="08F934237C3FD158" modified="2001-10-01" version="1">MPLYVIDKPITLHILTQLRDKYTDQINFRKNLVRLGRILGYEISNTLDYEIVEVETPLGVKTKGVDITDLNNIVIINILRAAVPLVEGLLKAFPKARQGVIGASRVEVDGKEVPKDMDVYIYYKKIPDIRAKVDNVIIADPMIATASTMLKVLEEVVKANPKRIYIVSIISSEYGVNKILSKYPFIYLFTVAIDPELNNKGYILPGLGDAGDRAFG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-05-10" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Q6</accession>
    <name>RL24E_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L24e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl24e</name>
        <name type="ordered locus">SSO5478</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90164">
        <property type="entry name" value="E90164"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990483.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Q6"/>
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    <dbReference type="FunCoup" id="Q980Q6">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO5478"/>
    <dbReference type="PaxDb" id="273057-SSO5478"/>
    <dbReference type="EnsemblBacteria" id="AAK40572">
        <property type="protein sequence ID" value="AAK40572"/>
        <property type="gene ID" value="SSO5478"/>
    </dbReference>
    <dbReference type="GeneID" id="15298283"/>
    <dbReference type="KEGG" id="sso:SSO5478"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.227"/>
    <dbReference type="eggNOG" id="arCOG01950">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_190191_0_0_2"/>
    <dbReference type="InParanoid" id="Q980Q6"/>
    <dbReference type="PhylomeDB" id="Q980Q6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000001">
        <property type="entry name" value="probable ribosome biogenesis protein RLP24"/>
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    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00773">
        <property type="entry name" value="Ribosomal_eL24"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L24e/L24_sf"/>
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        <property type="entry name" value="Ribosomal_eL24"/>
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        <property type="entry name" value="Ribosomal_eL24-rel_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_eL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
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        <property type="entry name" value="PRK14891.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000136928" description="Large ribosomal subunit protein eL24">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="61" mass="7321" checksum="9A3A7918B74A2F84" modified="2001-10-01" version="1">MPTTRQCSFCGHEIPPGTGLMYVRNDGTILWFCSSKCRKSMLKYHRDPKKYKWTTRYMKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q980R5</accession>
    <name>Q980R5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BFN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90163">
        <property type="entry name" value="B90163"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980R5"/>
    <dbReference type="STRING" id="273057.SSO0218"/>
    <dbReference type="PaxDb" id="273057-SSO0218"/>
    <dbReference type="EnsemblBacteria" id="AAK40561">
        <property type="protein sequence ID" value="AAK40561"/>
        <property type="gene ID" value="SSO0218"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0218"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.216"/>
    <dbReference type="eggNOG" id="arCOG01759">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131318_0_0_2"/>
    <dbReference type="InParanoid" id="Q980R5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.690.10">
        <property type="entry name" value="Bifunctional nuclease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036104">
        <property type="entry name" value="BFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003729">
        <property type="entry name" value="Bi_nuclease_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02577">
        <property type="entry name" value="BFN_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103256">
        <property type="entry name" value="Hypothetical protein TM0160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51658">
        <property type="entry name" value="BFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BFN" evidence="1">
        <location>
            <begin position="12"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="159" mass="18381" checksum="41DC2B94E3373EA8" modified="2001-10-01" version="1">MMSLQPIKDEDYIKVNSVDAFFMPLHGIPTIVCYLEDGRQFYLFSVPAEIVIAINKTKGNKEEEFGDKRENIYDIISFIPEITEEFSKHIEKVIIDDMIRDTGVYVATVEFKFDGVIIQKRMIPSHAIFLAIISNKPIFVKKGLVDEQEKESREGQQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q980S5</accession>
    <name>Q980S5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">D-arabino 3-hexulose 6-phosphate formaldehyde lyase (HpS-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hpS-2</name>
        <name evidence="4" type="ordered locus">SSO0202</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90161">
        <property type="entry name" value="E90161"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990446.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980S5"/>
    <dbReference type="FunCoup" id="Q980S5">
        <property type="interactions" value="189"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0202"/>
    <dbReference type="PaxDb" id="273057-SSO0202"/>
    <dbReference type="EnsemblBacteria" id="AAK40548">
        <property type="protein sequence ID" value="AAK40548"/>
        <property type="gene ID" value="SSO0202"/>
    </dbReference>
    <dbReference type="GeneID" id="1455358"/>
    <dbReference type="KEGG" id="sso:SSO0202"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.202"/>
    <dbReference type="eggNOG" id="arCOG00053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081825_1_1_2"/>
    <dbReference type="InParanoid" id="Q980S5"/>
    <dbReference type="PhylomeDB" id="Q980S5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033982">
        <property type="term" value="F:3-dehydro-L-gulonate-6-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019854">
        <property type="term" value="P:L-ascorbic acid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04726">
        <property type="entry name" value="KGPDC_HPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000022">
        <property type="entry name" value="3-keto-L-gulonate-6-phosphate decarboxylase UlaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041710">
        <property type="entry name" value="HPS/KGPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35039">
        <property type="entry name" value="3-KETO-L-GULONATE-6-PHOSPHATE DECARBOXYLASE SGBH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35039:SF3">
        <property type="entry name" value="3-KETO-L-GULONATE-6-PHOSPHATE DECARBOXYLASE SGBH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orotidine 5'-phosphate decarboxylase" evidence="3">
        <location>
            <begin position="14"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="225" mass="24803" checksum="778225487291DD6C" modified="2001-10-01" version="1">MREVLLEKLRTEKFLQIALDFIDINDAIKVVNEVKDLENEIIEIGTPLLKSFGIEGIRKIKSLVPDKIIVADTKTADAGDVEVEIAKLGGAHIMTVLGIMDDSTIYSAVKKARELDILVQADLINVKNVYERSKELKQLGVDIIGFHVGLDVQKSRGISVADLKNEIRKVSELNLIISIAGGLNKDRILELIDLPVSIYVVGGAITRSKEPKNVSKEIVNILKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="143" xmlns="https://uniprot.org/uniprot">
    <accession>Q980U9</accession>
    <name>Q980U9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AAA family ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO0176</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90158">
        <property type="entry name" value="E90158"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990405.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980U9"/>
    <dbReference type="FunCoup" id="Q980U9">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0176"/>
    <dbReference type="PaxDb" id="273057-SSO0176"/>
    <dbReference type="EnsemblBacteria" id="AAK40524">
        <property type="protein sequence ID" value="AAK40524"/>
        <property type="gene ID" value="SSO0176"/>
    </dbReference>
    <dbReference type="GeneID" id="1455334"/>
    <dbReference type="KEGG" id="sso:SSO0176"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.174"/>
    <dbReference type="eggNOG" id="arCOG01308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_12_2_2"/>
    <dbReference type="InParanoid" id="Q980U9"/>
    <dbReference type="PhylomeDB" id="Q980U9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000007">
        <property type="entry name" value="AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000057">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000008">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000178">
        <property type="entry name" value="CDC48/VCP homolog, AAA superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
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            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
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                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
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</entry>
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                <person name="Singh R.K."/>
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                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="143" xmlns="https://uniprot.org/uniprot">
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                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
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                <person name="Kozera C.J."/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="Helicase_ATP-bd"/>
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        <property type="entry name" value="Helicase_C-like"/>
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        <property type="entry name" value="MZB"/>
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        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
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        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
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    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
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    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
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        <property type="entry name" value="MZB"/>
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        <property type="entry name" value="DEXDc"/>
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    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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        <property type="entry name" value="HELICASE_CTER"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="64"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="264"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="739" mass="83674" checksum="A4D5BF9AFFDA2C74" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-07-11" modified="2026-01-28" version="146" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX24</accession>
    <name>PURL_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit PurL</fullName>
            <shortName evidence="1">FGAM synthase</shortName>
            <ecNumber evidence="1">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formylglycinamide ribonucleotide amidotransferase subunit II</fullName>
            <shortName evidence="1">FGAR amidotransferase II</shortName>
            <shortName evidence="1">FGAR-AT II</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutamine amidotransferase PurL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purL</name>
        <name type="ordered locus">SSO0629</name>
        <name type="ORF">C08_019</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGAMS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90210">
        <property type="entry name" value="E90210"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991165.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX24"/>
    <dbReference type="SMR" id="Q9UX24"/>
    <dbReference type="FunCoup" id="Q9UX24">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0629"/>
    <dbReference type="PaxDb" id="273057-SSO0629"/>
    <dbReference type="EnsemblBacteria" id="AAK40940">
        <property type="protein sequence ID" value="AAK40940"/>
        <property type="gene ID" value="SSO0629"/>
    </dbReference>
    <dbReference type="GeneID" id="1454907"/>
    <dbReference type="GeneID" id="44129631"/>
    <dbReference type="KEGG" id="sso:SSO0629"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.636"/>
    <dbReference type="eggNOG" id="arCOG00641">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003100_0_1_2"/>
    <dbReference type="InParanoid" id="Q9UX24"/>
    <dbReference type="PhylomeDB" id="Q9UX24"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02203">
        <property type="entry name" value="PurL_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02204">
        <property type="entry name" value="PurL_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00420">
        <property type="entry name" value="PurL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010074">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01736">
        <property type="entry name" value="FGAM_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002290">
        <property type="entry name" value="PRK01213.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001587">
        <property type="entry name" value="FGAM_synthase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000100525" description="Phosphoribosylformylglycinamidine synthase subunit PurL">
        <location>
            <begin position="1"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="83"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="294"/>
            <end position="296"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="507"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="510"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00420"/>
        </source>
    </evidence>
    <sequence length="709" mass="77089" checksum="150777DE8D23E5CA" modified="2000-05-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX89</accession>
    <name>RL19E_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L19e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl19e</name>
        <name type="ordered locus">SSO0700</name>
        <name type="ORF">C10_030</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90218">
        <property type="entry name" value="B90218"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991257.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX89"/>
    <dbReference type="SMR" id="Q9UX89"/>
    <dbReference type="FunCoup" id="Q9UX89">
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    </dbReference>
    <dbReference type="STRING" id="273057.SSO0700"/>
    <dbReference type="PaxDb" id="273057-SSO0700"/>
    <dbReference type="EnsemblBacteria" id="AAK41001">
        <property type="protein sequence ID" value="AAK41001"/>
        <property type="gene ID" value="SSO0700"/>
    </dbReference>
    <dbReference type="GeneID" id="1454968"/>
    <dbReference type="KEGG" id="sso:SSO0700"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.700"/>
    <dbReference type="eggNOG" id="arCOG04089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083919_1_1_2"/>
    <dbReference type="InParanoid" id="Q9UX89"/>
    <dbReference type="PhylomeDB" id="Q9UX89"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01418">
        <property type="entry name" value="Ribosomal_L19e_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.240:FF:000003">
        <property type="entry name" value="50S ribosomal protein L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_eL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033936">
        <property type="entry name" value="Ribosomal_eL19_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023638">
        <property type="entry name" value="Ribosomal_eL19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00526">
        <property type="entry name" value="RIBOSOMAL_L19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000131196" description="Large ribosomal subunit protein eL19">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="62"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="151" mass="17286" checksum="9F65D14F5AE7760B" modified="2000-05-01" version="1">MTNLKAQKRLAASIAGVGINRIKVVEDYIEDVQSSLTRDEIRNLIKDGKIIVLKKVGISGGRLKERRKKRSLKSEGKKSGSRKGKKGARANSKQMWVKRVRKIRAYLKWLRDHKVIDRHTYRELYLKTKGGNYKGVSDVRNVLIQMGKIKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-20" modified="2025-06-18" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>P58178</accession>
    <name>TBP_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>TATA-box-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Box A-binding protein</fullName>
            <shortName>BAP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>TATA sequence-binding protein</fullName>
            <shortName>TBP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>TATA-box factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">tbp</name>
        <name type="synonym">tfiID</name>
        <name type="ordered locus">SSO0951</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBP family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK41225" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41225.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90246">
        <property type="entry name" value="B90246"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58178"/>
    <dbReference type="SMR" id="P58178"/>
    <dbReference type="FunCoup" id="P58178">
        <property type="interactions" value="209"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0951"/>
    <dbReference type="PaxDb" id="273057-SSO0951"/>
    <dbReference type="EnsemblBacteria" id="AAK41225">
        <property type="protein sequence ID" value="AAK41225"/>
        <property type="gene ID" value="SSO0951"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0951"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.947"/>
    <dbReference type="eggNOG" id="arCOG01764">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060161_4_3_2"/>
    <dbReference type="InParanoid" id="P58178"/>
    <dbReference type="PhylomeDB" id="P58178"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140223">
        <property type="term" value="F:general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04518">
        <property type="entry name" value="TBP_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000007">
        <property type="entry name" value="TATA-box-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000010">
        <property type="entry name" value="TATA-box-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033711">
        <property type="entry name" value="TBP_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001592">
        <property type="entry name" value="PRK00394.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001593">
        <property type="entry name" value="PRK00394.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000154026" description="TATA-box-binding protein">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="1">
        <location>
            <begin position="14"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="2">
        <location>
            <begin position="105"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="198" mass="22309" checksum="231448D0B2B026D7" modified="2001-06-20" version="1">MSNSAVSYKPIVNIENIVATVTLEQSLDLYAMERSIPNIEYDPDQFPGLIFRLEQPKVTALIFKSGKMVVTGAKSTEELIKAVKRIIKTLKKYGIKIVGKPKIQIQNIVASANLHVNVNLDKAAFLLENNMYEPEQFPGLIFRMDDPRVVLLIFSSGKMVITGAKREDEVSKAVKRIFDKLAELDCVKPIEEEEELEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="142" xmlns="https://uniprot.org/uniprot">
    <accession>P96041</accession>
    <name>SYA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="1">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">alaS</name>
        <name type="ordered locus">SSO0341</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Biochim. Biophys. Acta" volume="1340" first="170" last="177">
            <title>Nucleotide sequence of a gene cluster encoding NusG and the L11-L1-L10-L12 ribosomal proteins from the thermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Geiger M."/>
                <person name="Groebner P."/>
                <person name="Piendl W."/>
            </authorList>
            <dbReference type="PubMed" id="9252104"/>
            <dbReference type="DOI" id="10.1016/S0167-4838(97)00073-3"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-174</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="U85262">
        <property type="protein sequence ID" value="AAB99528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90176">
        <property type="entry name" value="H90176"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990629.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P96041"/>
    <dbReference type="SMR" id="P96041"/>
    <dbReference type="FunCoup" id="P96041">
        <property type="interactions" value="221"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0341"/>
    <dbReference type="PaxDb" id="273057-SSO0341"/>
    <dbReference type="EnsemblBacteria" id="AAK40671">
        <property type="protein sequence ID" value="AAK40671"/>
        <property type="gene ID" value="SSO0341"/>
    </dbReference>
    <dbReference type="GeneID" id="1455481"/>
    <dbReference type="GeneID" id="44129312"/>
    <dbReference type="KEGG" id="sso:SSO0341"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.332"/>
    <dbReference type="eggNOG" id="arCOG01255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_4_0_2"/>
    <dbReference type="InParanoid" id="P96041"/>
    <dbReference type="PhylomeDB" id="P96041"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00673">
        <property type="entry name" value="AlaRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.130:FF:000010">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.54.20:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000056">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.54.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_A">
        <property type="entry name" value="Ala_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022429">
        <property type="entry name" value="Ala-tRNA_lgiase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03683">
        <property type="entry name" value="A-tRNA_syn_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000075277" description="Alanine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="604"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="608"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="708"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="712"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <sequence length="900" mass="102980" checksum="6CB15A31210E153F" modified="2001-06-01" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="171" xmlns="https://uniprot.org/uniprot">
    <accession>Q59969</accession>
    <name>CARB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carbamoyl phosphate synthase large chain</fullName>
            <ecNumber evidence="1">6.3.4.16</ecNumber>
            <ecNumber evidence="1">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Carbamoyl phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">carB</name>
        <name type="ordered locus">SSO0641</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Biol. Evol." volume="13" first="970" last="977">
            <title>Phylogenetic analysis of carbamoylphosphate synthetase genes: complex evolutionary history includes an internal duplication within a gene which can root the tree of life.</title>
            <authorList>
                <person name="Lawson F.S."/>
                <person name="Charlebois R.L."/>
                <person name="Dillon J.A."/>
            </authorList>
            <dbReference type="PubMed" id="8752005"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Large subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The large subunit (synthetase) binds the substrates ammonia (free or transferred from glutamine from the small subunit), hydrogencarbonate and ATP and carries out an ATP-coupled ligase reaction, activating hydrogencarbonate by forming carboxy phosphate which reacts with ammonia to form carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <molecule>Carbamoyl phosphate synthase large chain</molecule>
        <reaction evidence="1">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:17544"/>
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        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 4 Mg(2+) or Mn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The large subunit is composed of 2 ATP-grasp domains that are involved in binding the 2 ATP molecules needed for carbamoyl phosphate synthesis. The N-terminal ATP-grasp domain (referred to as the carboxyphosphate synthetic component) catalyzes the ATP-dependent phosphorylation of hydrogencarbonate to carboxyphosphate and the subsequent nucleophilic attack by ammonia to form a carbamate intermediate. The C-terminal ATP-grasp domain (referred to as the carbamoyl phosphate synthetic component) then catalyzes the phosphorylation of carbamate with the second ATP to form the end product carbamoyl phosphate. The reactive and unstable enzyme intermediates are sequentially channeled from one active site to the next through the interior of the protein over a distance of at least 96 A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CarB family.</text>
    </comment>
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        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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        </location>
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</entry>
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            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
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                <person name="Clausen I.G."/>
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                <person name="Heikamp-de Jong I."/>
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        <property type="entry name" value="MFS_trans_sf"/>
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    </dbReference>
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    </feature>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXD8</accession>
    <name>Q7LXD8_SACS2</name>
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        <recommendedName>
            <fullName evidence="1">Transposase IS116/IS110/IS902 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    <organism evidence="2 3">
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90234">
        <property type="entry name" value="H90234"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXD8"/>
    <dbReference type="PaxDb" id="273057-SSO0844"/>
    <dbReference type="EnsemblBacteria" id="AAK41135">
        <property type="protein sequence ID" value="AAK41135"/>
        <property type="gene ID" value="SSO0844"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0844"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.855"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146471_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXD8"/>
    <dbReference type="PhylomeDB" id="Q7LXD8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="1">
        <location>
            <begin position="61"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="103" mass="11861" checksum="C866E81881E7C437" modified="2011-05-31" version="1">MGYSDSLSRKNLSEVLEGGDNIILSEVKFLLDELERLEARKREVEKRLEDVVPRDSLIFRKVLGLIILARVGDVRRFDDKRKFVAYCGLDSVECCILRCLKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXE1</accession>
    <name>Q7LXE1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TRM5/TYW2-like methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0838</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90234">
        <property type="entry name" value="E90234"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923127.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXE1"/>
    <dbReference type="STRING" id="273057.SSO0838"/>
    <dbReference type="PaxDb" id="273057-SSO0838"/>
    <dbReference type="EnsemblBacteria" id="AAK41132">
        <property type="protein sequence ID" value="AAK41132"/>
        <property type="gene ID" value="SSO0838"/>
    </dbReference>
    <dbReference type="GeneID" id="1455099"/>
    <dbReference type="KEGG" id="sso:SSO0838"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.852"/>
    <dbReference type="eggNOG" id="arCOG01401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101256_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXE1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052514">
        <property type="entry name" value="SAM-dependent_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203">
        <property type="entry name" value="METHYLTRANSFERASE, FKBM FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203:SF15">
        <property type="entry name" value="SLL1173 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRM5/TYW2-like methyltransferase" evidence="3">
        <location>
            <begin position="33"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="239" mass="27177" checksum="CBFC36DB2CDF269E" modified="2011-05-31" version="1">MIFTYGKTKLKVPEGYEYVYYATFIAAEWDFLKVKDMDTVLDAGAFIGDFTVKVARKAKEVVAVEPLPWAFKLLKENVEINNLKNVVLVNKALYDVDGVKLKIVDEGTGSRIGESGVEVDSVSVNSLGKFSVVKMDIEGAEGRVMKSGEWLDYVKQIAVELHGRENIEIIPHLLRKRSFVIRFMTRGDLVRNTVRNALLHPISFIKAEARTKVVLNYFKRKYYVPALSGDEYKIVYGRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2026-01-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXJ7</accession>
    <name>GIN23_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA replication complex GINS protein Gins23</fullName>
            <shortName evidence="3">Gins23</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gins23</name>
        <name evidence="6" type="ordered locus">SSO0772</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="EMBO Rep." volume="7" first="539" last="545">
            <title>GINS, a central nexus in the archaeal DNA replication fork.</title>
            <authorList>
                <person name="Marinsek N."/>
                <person name="Barry E.R."/>
                <person name="Makarova K.S."/>
                <person name="Dionne I."/>
                <person name="Koonin E.V."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="16485022"/>
            <dbReference type="DOI" id="10.1038/sj.embor.7400649"/>
        </citation>
        <scope>INTERACTION WITH GINS15; MCM; PRIL AND PRIS</scope>
        <scope>INDUCTION</scope>
        <scope>OPERON STRUCTURE</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="journal article" date="2025" name="Acta Crystallogr. F Struct. Biol. Commun." volume="81" first="207" last="215">
            <title>Structure of the Saccharolobus solfataricus GINS tetramer.</title>
            <authorList>
                <person name="Shankar S."/>
                <person name="Enemark E.J."/>
            </authorList>
            <dbReference type="PubMed" id="40235367"/>
            <dbReference type="DOI" id="10.1107/s2053230x25003085"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS)</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The CG complex (Cdc45, Gins15 and Gins23) stimulates the helicase activity of the MCM replicative DNA helicase; it copurifies throughout the cell cycle with MCM (as presumably does the CG complex) probably associated with moving replication forks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the Cdc45-GINS complex (CG complex) that includes Cdc45, Gins15 and Gins23 (PubMed:16485022). The GINS complex has 2:2 stoichiometry (PubMed:16485022, PubMed:40235367). Interacts directly with Gins15. Gins23 interacts with N-terminal 226 residues of MCM in all growth phases (PubMed:16485022).</text>
    </comment>
    <comment type="induction">
        <text evidence="2">Expressed during early, mid- and late-logarithmic growth (at protein level). Encoded in the gins23-MCM operon.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GINS1/PSF1 family.</text>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK41070" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41070.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90226">
        <property type="entry name" value="G90226"/>
    </dbReference>
    <dbReference type="PDB" id="9MOJ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="C/D=1-179"/>
    </dbReference>
    <dbReference type="PDBsum" id="9MOJ"/>
    <dbReference type="AlphaFoldDB" id="Q7LXJ7"/>
    <dbReference type="STRING" id="273057.SSO0772"/>
    <dbReference type="PaxDb" id="273057-SSO0772"/>
    <dbReference type="EnsemblBacteria" id="AAK41070">
        <property type="protein sequence ID" value="AAK41070"/>
        <property type="gene ID" value="SSO0772"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0772"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.773"/>
    <dbReference type="eggNOG" id="arCOG00552">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117967_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXJ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd11714">
        <property type="entry name" value="GINS_A_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038437">
        <property type="entry name" value="GINS_Psf3_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000463483" description="DNA replication complex GINS protein Gins23">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q4JAB6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16485022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="16485022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="40235367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9MOJ"/>
        </source>
    </evidence>
    <sequence length="179" mass="20621" checksum="DD69545C1A092446" modified="2026-01-28" version="2">MIEVKLRAIKRLSNVYTRRVMIIEDWNGSSITTGNIELVKGSENQLPQWLAIILEGKKVAKIEDKISIEDLGRILFQERQNMNTPASLVPLGKDFTSRVQLYLETLRKDNNVESLEKLRKSIGILNEIIKIRLRKLIQLAFLNIDDQNLINGMTEEELLIYKTIKQLIKELYGDIIGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXQ9</accession>
    <name>Q7LXQ9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Haloacid dehalogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0646</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90211">
        <property type="entry name" value="H90211"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991182.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXQ9"/>
    <dbReference type="STRING" id="273057.SSO0646"/>
    <dbReference type="PaxDb" id="273057-SSO0646"/>
    <dbReference type="EnsemblBacteria" id="AAK40951">
        <property type="protein sequence ID" value="AAK40951"/>
        <property type="gene ID" value="SSO0646"/>
    </dbReference>
    <dbReference type="GeneID" id="1454918"/>
    <dbReference type="KEGG" id="sso:SSO0646"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.648"/>
    <dbReference type="eggNOG" id="arCOG04318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099315_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXQ9"/>
    <dbReference type="PhylomeDB" id="Q7LXQ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14820">
        <property type="entry name" value="TRAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036081">
        <property type="entry name" value="Translin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011156">
        <property type="entry name" value="PRK14562.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74784">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="22810" checksum="56D8CE5230428237" modified="2011-05-31" version="1">MLVEKIKGYLNSISGVLQERFENRERVLMLARELIRYCGETISLSHKGKKEEALKKYQIAISKVSEIQKIIDSFPELLYGDVGTAYQELAEASIVISLYFDVELKLSKELGIPDIYYISGIADAIGEMRRRVLELLKKNKIDEAENTYNIMEELYELLWSFEYPKSLVPGLRQKIDTIRRLLEETNHDIFLAKLGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXS3</accession>
    <name>Q7LXS3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0609</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90208">
        <property type="entry name" value="A90208"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991135.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXS3"/>
    <dbReference type="STRING" id="273057.SSO0609"/>
    <dbReference type="PaxDb" id="273057-SSO0609"/>
    <dbReference type="EnsemblBacteria" id="AAK40920">
        <property type="protein sequence ID" value="AAK40920"/>
        <property type="gene ID" value="SSO0609"/>
    </dbReference>
    <dbReference type="GeneID" id="1454887"/>
    <dbReference type="KEGG" id="sso:SSO0609"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.617"/>
    <dbReference type="eggNOG" id="arCOG05995">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2366034_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXS3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="99" mass="11315" checksum="BA869A3580DB4AE1" modified="2011-05-31" version="1">MVRPVMKGRDLETLVFLGRLKEVKVEYCDEEKKMAKVIGITDTKEEVETECIPLRAAGKISTVLKHYIRLGVGNYIITQTDMRNVANVDTQAEEEEKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXV9</accession>
    <name>Q7LXV9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0539</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90200">
        <property type="entry name" value="C90200"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991035.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXV9"/>
    <dbReference type="PaxDb" id="273057-SSO0539"/>
    <dbReference type="EnsemblBacteria" id="AAK40858">
        <property type="protein sequence ID" value="AAK40858"/>
        <property type="gene ID" value="SSO0539"/>
    </dbReference>
    <dbReference type="GeneID" id="1454825"/>
    <dbReference type="KEGG" id="sso:SSO0539"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.538"/>
    <dbReference type="eggNOG" id="arCOG07527">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120799_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXV9"/>
    <dbReference type="PhylomeDB" id="Q7LXV9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="134" mass="15649" checksum="77A10B45CB543EC2" modified="2011-05-31" version="1">MPQSLKPKSIKLLHYEEKKDDKRIFRQGVTLIEYEGQPSKIIQWSQLIEGDPFGDHEITYRINYGSESELRSFKVKYLGREGDKHRVLIKEGVSGCGTKTKRIENKEVLISDKLYQPYPLQQKNEEGRDPNPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-06-18" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXX6</accession>
    <name>Q7LXX6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type II secretion system protein, virB homolog (GspE-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gspE-1</name>
        <name evidence="3" type="ordered locus">SSO0120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75369">
        <property type="entry name" value="S75369"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXX6"/>
    <dbReference type="STRING" id="273057.SSO0120"/>
    <dbReference type="PaxDb" id="273057-SSO0120"/>
    <dbReference type="EnsemblBacteria" id="AAK40475">
        <property type="protein sequence ID" value="AAK40475"/>
        <property type="gene ID" value="SSO0120"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0120"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.115"/>
    <dbReference type="eggNOG" id="arCOG01817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_2_2_2"/>
    <dbReference type="InParanoid" id="Q7LXX6"/>
    <dbReference type="PhylomeDB" id="Q7LXX6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01130">
        <property type="entry name" value="VirB11-like_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF6">
        <property type="entry name" value="TYPE IV PILUS RETRACTATION ATPASE PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial type II secretion system protein E" evidence="2">
        <location>
            <begin position="141"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="483" mass="55370" checksum="C7FC726CA28B7ECF" modified="2011-05-31" version="1">MSIFFLNNGIMNILQEYSVLNAKVIIYEENGIGYYKVNEPNLDQNEIEILNSILEYIYSTPSVPNIEETVVKILKNKGISDQGIIEKIIYYLRKRIGYDELTVPLADPYVEEIECKGFSYPVTVVHRIVTRFPRLYTNIILEQEDHVLKVIEKLANKADKPVSIAKPYLEFSLPEGHRVAATVSREVSLPGSTFDIRKFPLKPISPISLIKNESISSLMLAYIWFLLDYKPFFLIIGSTGSGKTTLLNSILSMINPFFKVITIEDTPEINIIHDNWIRFFARQSISSQFEVSLMDLAKLSLRYRPDYLVIGEIRGKEIEALVHASASGHGSLATFHAGNPHEALTRLLSLLNKDVAKLFLQNLWGIIVLGTLRDRNGNIRRIIRSIYEIVYTRGKLRFRPVLRWSFSSNTFMPNNIDDLIKRSYRISYISKTYEIPISEITKEISNRVKTLNNFVSNNILDLEEFSVYLKKISQGDKVETKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXY2</accession>
    <name>Q7LXY2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO0114</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75375">
        <property type="entry name" value="S75375"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXY2"/>
    <dbReference type="STRING" id="273057.SSO0114"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO0114"/>
    <dbReference type="EnsemblBacteria" id="AAK40468">
        <property type="protein sequence ID" value="AAK40468"/>
        <property type="gene ID" value="SSO0114"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0114"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.108"/>
    <dbReference type="eggNOG" id="arCOG01391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041759_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LXY2"/>
    <dbReference type="PhylomeDB" id="Q7LXY2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050321">
        <property type="entry name" value="Glycosyltr_2/OpgH_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43867">
        <property type="entry name" value="CELLULOSE SYNTHASE CATALYTIC SUBUNIT A [UDP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43867:SF2">
        <property type="entry name" value="CELLULOSE SYNTHASE CATALYTIC SUBUNIT A [UDP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="8">
        <location>
            <begin position="50"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK40468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="458" mass="51578" checksum="FA3FBD3F6BB2740E" modified="2011-05-31" version="1">MILNILLNLAIFIIPSLAVWNQIILYIFGKSGDIANLILLNNESNLPKLSIIVPTKGERIEVIQGLIDNIHEAIWDRNKLEIIIVSDDEQEYFDKLLSTLIIPPDLDVRIFRREKKLGYKSGALAFGLQKSTGDLILTLDVDARIEKDSLIKAYNHMVNLGCDAITMEWHGYSNISTSLAKALMVSTVLTSKSILRGRDKLGLKVLPIGCGTIYKRSALEAVNGWDYKMIQDDYELGARLINKGFKVCASSSPVYVEVPDNLIAFYVQQTRWAMGTMEVLYWRFKYIISSDIKLWQKLEIIAYLSQYIPIILTFISAIIFAIAGFVGIRLSMNLPMFIIWAITLSIYASIFVNSAKKSGIDTVTAIKALGRLSAYTVGISPFLLIGTINAFKKTRTYIVTPKGKKAKSNIGYPILAFGVFFILSAFLYMFRGDFLTFIWLAYYSIAFLYTFIAYIKGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-01-25" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LYI9</accession>
    <name>GLCD1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucose 1-dehydrogenase 1</fullName>
            <shortName evidence="1">GDH 1</shortName>
            <shortName evidence="1">GlcDH 1</shortName>
            <ecNumber evidence="1">1.1.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gdh1</name>
        <name type="synonym">dhg-1</name>
        <name type="ordered locus">SSO3003</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NAD(P)(+)-dependent oxidation of D-glucose to D-gluconate via gluconolactone. Can utilize both NAD(+) and NADP(+) as electron acceptor. Is involved in the degradation of glucose through a non-phosphorylative variant of the Entner-Doudoroff pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose + NAD(+) = D-glucono-1,5-lactone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14293"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16217"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.47"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose + NADP(+) = D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14405"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16217"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit. One of the zinc atoms is essential for catalytic activity while the other has a structural function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family. Glucose 1-dehydrogenase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.47" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="T44937">
        <property type="entry name" value="T44937"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992653.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LYI9"/>
    <dbReference type="SMR" id="Q7LYI9"/>
    <dbReference type="FunCoup" id="Q7LYI9">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3003"/>
    <dbReference type="PaxDb" id="273057-SSO3003"/>
    <dbReference type="EnsemblBacteria" id="AAK43106">
        <property type="protein sequence ID" value="AAK43106"/>
        <property type="gene ID" value="SSO3003"/>
    </dbReference>
    <dbReference type="GeneID" id="1453025"/>
    <dbReference type="KEGG" id="sso:SSO3003"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3095"/>
    <dbReference type="eggNOG" id="arCOG01459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LYI9"/>
    <dbReference type="PhylomeDB" id="Q7LYI9"/>
    <dbReference type="BRENDA" id="1.1.1.359">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047910">
        <property type="term" value="F:galactose 1-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005534">
        <property type="term" value="F:galactose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047934">
        <property type="term" value="F:glucose 1-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047935">
        <property type="term" value="F:glucose 1-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033498">
        <property type="term" value="P:galactose catabolic process via D-galactonate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019595">
        <property type="term" value="P:non-phosphorylated glucose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08230">
        <property type="entry name" value="glucose_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000957">
        <property type="entry name" value="Glucose 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000074">
        <property type="entry name" value="Glucose 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02127">
        <property type="entry name" value="Glucose_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026583">
        <property type="entry name" value="Glc_1-DH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031640">
        <property type="entry name" value="Glu_dehyd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF2">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16912">
        <property type="entry name" value="Glu_dehyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000414840" description="Glucose 1-dehydrogenase 1">
        <location>
            <begin position="1"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
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            <position position="150"/>
        </location>
        <ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
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            <label>1</label>
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        </ligand>
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        <location>
            <begin position="189"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="211"/>
            <end position="213"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="277"/>
            <end position="279"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="305"/>
            <end position="307"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02127"/>
        </source>
    </evidence>
    <sequence length="366" mass="40891" checksum="59D4AC33B447D3EB" modified="2011-05-31" version="1">MKAIIVKPPNAGVQVKDVDEKKLDSYGKIKIRTIYNGICGTDREIVNGKLTLSTLPKGKDFLVLGHEAIGVVEESYHGFSQGDLVMPVNRRGCGICRNCLVGRPDFCETGEFGEAGIHKMDGFMREWWYDDPKYLVKIPKSIEDIGILAQPLADIEKSIEEILEVQKRVPVWTCDDGTLNCRKVLVVGTGPIGVLFTLLFRTYGLEVWMANRREPTEVEQTVIEETKTNYYNSSNGYDKLKDSVGKFDVIIDATGADVNILGNVIPLLGRNGVLGLFGFSTSGSVPLDYKTLQEIVHTNKTIIGLVNGQKPHFQQAVVHLASWKTLYPKAAKMLITKTVSINDEKELLKVLREKEHGEIKIRILWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TX3</accession>
    <name>Q97TX3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Second ORF in transposon ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1708</name>
        <name evidence="4" type="ordered locus">SSO1791</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90332">
        <property type="entry name" value="B90332"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TX3"/>
    <dbReference type="STRING" id="273057.SSO1708"/>
    <dbReference type="PaxDb" id="273057-SSO1708"/>
    <dbReference type="EnsemblBacteria" id="AAK41913">
        <property type="protein sequence ID" value="AAK41913"/>
        <property type="gene ID" value="SSO1708"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41985">
        <property type="protein sequence ID" value="AAK41985"/>
        <property type="gene ID" value="SSO1791"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1708"/>
    <dbReference type="KEGG" id="sso:SSO1791"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1757"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036902_12_2_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="2">
        <location>
            <begin position="12"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="131" mass="15051" checksum="023BCCB2BEBB2DF7" modified="2001-10-01" version="1">MKSKNFPEILVLLTISGIGKLAAGIIIGIVGDIRRFPRPESFVAYCGLDPVVERSGRIEVRKGISKRGNKYLRSLFYFLAETQYSRNPTLLKLHEAHKDKLKGRKLYTALARKLARIVWSVWYNNKPYEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U46</accession>
    <name>Q97U46_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90502">
        <property type="entry name" value="E90502"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U46"/>
    <dbReference type="PaxDb" id="273057-SSO3178"/>
    <dbReference type="EnsemblBacteria" id="AAK43276">
        <property type="protein sequence ID" value="AAK43276"/>
        <property type="gene ID" value="SSO3178"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3178"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3282"/>
    <dbReference type="eggNOG" id="arCOG07249">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174525_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U46"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="108" mass="12316" checksum="2162BEA4D1B550D9" modified="2001-10-01" version="1">MKKSLDFCMFQNIFIHKMRVFIMAVEFNEKGVTIKIPTLSTSISFSKDQIEKVEEVVPPDEICRFARNSGVIFAGSTIDGKVMYFNVKKGERCLLLVLKDGRKVYIGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U59</accession>
    <name>Q97U59_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycolate oxidase glcE subunit. (GlcE)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">glcE</name>
        <name evidence="3" type="ordered locus">SSO3165</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90500">
        <property type="entry name" value="H90500"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U59"/>
    <dbReference type="STRING" id="273057.SSO3165"/>
    <dbReference type="PaxDb" id="273057-SSO3165"/>
    <dbReference type="EnsemblBacteria" id="AAK43263">
        <property type="protein sequence ID" value="AAK43263"/>
        <property type="gene ID" value="SSO3165"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3165"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3268"/>
    <dbReference type="eggNOG" id="arCOG00344">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_722834_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U59"/>
    <dbReference type="PhylomeDB" id="Q97U59"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF111">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="2">
        <location>
            <begin position="40"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="382" mass="43043" checksum="290974B4D76848D2" modified="2001-10-01" version="1">MYIGINIILLIKNYFYNLSPFLLMDWVDELGKIVKVSNGKDEGPARWKITAIPQTYAELRDVIKFANEKKLSIYPFSFNMHHIGPPVSIDIGVKLSQFNNVVEISDDDLYVTSQVGVRVSDLFEIIKAKGLFLPAYYDGSLGGLLATNLPTPFSSFYGYPKNLILMAKVITGDGIIAKGGGKTLKFSSGYKIHKILSGMLGWLGIYLEATLKIYPFPEVIVTFQTDKVNLTSKYRPISIIYETDEKGDKTYVTFIGFRRAIEKIGEEIGAKGEDGFYSIDYTSDEKVISIHTVRGKEVDVIRKAKSIMRVKRGIGIVGTGYCRLEVASFDNLEVLRREIDEQVVVERGYYNGDYWGIRDKETLQMLKEAFDPNNILQPGLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U97</accession>
    <name>Q97U97_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99496">
        <property type="entry name" value="A99496"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989943.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U97"/>
    <dbReference type="STRING" id="273057.SSO3123"/>
    <dbReference type="PaxDb" id="273057-SSO3123"/>
    <dbReference type="EnsemblBacteria" id="AAK43224">
        <property type="protein sequence ID" value="AAK43224"/>
        <property type="gene ID" value="SSO3123"/>
    </dbReference>
    <dbReference type="GeneID" id="1453143"/>
    <dbReference type="KEGG" id="sso:SSO3123"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3229"/>
    <dbReference type="eggNOG" id="arCOG08494">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_616269_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U97"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="444" mass="51149" checksum="21CD965E66D075CD" modified="2001-10-01" version="1">MERIGDLLSNLPTDYAKALIQILTADNWNRLDRDVNFYQLGLGIGKVVSRIDKETLKALVKSCDYYQSLCRGIAKGMDGIELDRDLILYLGNLSPVMAMELLANLELYKYPDIMKILAVNVAQIKHIPNVGSNIARQFDKLPFEIRRQILDIFKDNSMFLYEFLQSVNLNKVDNIENFLNKIKEIDEIIGYRLYEVNDKMKEKLLNFSSISVGIGKGFQNLSYHWKRKVIEKVKKDKEFAKGFLSSIDLSLLEDEFFDIIIKIGESDLELSKVLGRNFGNSLAYLTEDLKSLAFNIAQGNPDFARGFGEGISESLGSFIGFIRGKAYELKKEDQDRVLDLALSNDNFANGLLTTFNAIFFFDNKEKVIELMIKREQYLKLFIEQIGRRINDFDLFKLLSLNNKLTSELGKILCRNFIYLSKKNREIVLEWLSKNNELKEGFLQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UA3</accession>
    <name>Q97UA3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO11986</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90495">
        <property type="entry name" value="C90495"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UA3"/>
    <dbReference type="STRING" id="273057.SSO11986"/>
    <dbReference type="PaxDb" id="273057-SSO11986"/>
    <dbReference type="EnsemblBacteria" id="AAK43218">
        <property type="protein sequence ID" value="AAK43218"/>
        <property type="gene ID" value="SSO11986"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11986"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3223"/>
    <dbReference type="eggNOG" id="arCOG02273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2766277_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UA3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="69" mass="8226" checksum="3A3C1B09CC7623BD" modified="2001-10-01" version="1">MMHVYKVKLDIKHGSCWTYKTSDFKVKAKVMYLFPLITKNSIFEIAEIYSNDKNELSDFISTINMKIWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UB3</accession>
    <name>Q97UB3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metal-dependent carboxypeptidase</fullName>
            <ecNumber evidence="1">3.4.17.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO3105</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Broad specificity carboxypetidase that releases amino acids sequentially from the C-terminus, including neutral, aromatic, polar and basic residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of a C-terminal amino acid with broad specificity, except for -Pro.</text>
            <dbReference type="EC" id="3.4.17.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M32 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.19" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99494">
        <property type="entry name" value="A99494"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990926.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UB3"/>
    <dbReference type="STRING" id="273057.SSO3105"/>
    <dbReference type="PaxDb" id="273057-SSO3105"/>
    <dbReference type="EnsemblBacteria" id="AAK43208">
        <property type="protein sequence ID" value="AAK43208"/>
        <property type="gene ID" value="SSO3105"/>
    </dbReference>
    <dbReference type="GeneID" id="1453127"/>
    <dbReference type="KEGG" id="sso:SSO3105"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3212"/>
    <dbReference type="eggNOG" id="arCOG04247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032916_1_1_2"/>
    <dbReference type="InParanoid" id="Q97UB3"/>
    <dbReference type="PhylomeDB" id="Q97UB3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06460">
        <property type="entry name" value="M32_Taq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001333">
        <property type="entry name" value="Peptidase_M32_Taq"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34217:SF1">
        <property type="entry name" value="CARBOXYPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34217">
        <property type="entry name" value="METAL-DEPENDENT CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02074">
        <property type="entry name" value="Peptidase_M32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006615">
        <property type="entry name" value="Zn_crbxpep_Taq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00998">
        <property type="entry name" value="CRBOXYPTASET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52034">
        <property type="entry name" value="PEPTIDASE_M32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR006615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR006615-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="467" mass="54841" checksum="55D5119687C36BF3" modified="2001-10-01" version="1">MYEDIWAIEHAISLLDWDIQTYMPQSGIKARGEALARLSNLRRKLLLGIRGEIEKLEPKNDIEKGLKRVLEREYKYYDAVPEELDMKLHRITSEATVVWRNAKAKGDFNAFKPYLEQILEIKREIAHKLGYKDHPYSALLDRYEEGFTVTDAERVFNELLPGLSKILNKIDDKFTRKYHFEDEKYDVFQMSKTIEAIAYEVLKMPKDRFRIDVSPHPFTVSMSRNDVRITVRYEGYDFKRVLYSLVHESGHAIYELQIDPSLEYSPLANAPSMGLHESQSRFWENVVGRSYGFIKTIYPLLNVKDSIDDVYYYVNGVKRQPIRVDADEVTYNFHIAIRYEIEKRAIEGSLEASEFPSLFNDLMDKYLNIRPKNDGEGVLQDVHWSQGSFGYFPTYTLGNVIAGMVYYHMKSERGFDISNIEGIKNWLRERIHKYGSIYSPKELQMRSFGEAYNPSRLLDYMREKYNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UB9</accession>
    <name>Q97UB9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90493">
        <property type="entry name" value="C90493"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989123.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UB9"/>
    <dbReference type="PaxDb" id="273057-SSO3098"/>
    <dbReference type="EnsemblBacteria" id="AAK43202">
        <property type="protein sequence ID" value="AAK43202"/>
        <property type="gene ID" value="SSO3098"/>
    </dbReference>
    <dbReference type="GeneID" id="84059588"/>
    <dbReference type="KEGG" id="sso:SSO3098"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3204"/>
    <dbReference type="eggNOG" id="arCOG06008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1232810_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UB9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="246" mass="26417" checksum="25F0BE0BD34C2A20" modified="2001-10-01" version="1">MRGKELIIALSISLGIAVAILSLPDILEPIAHKILLVLGIYWPLAIFIIGIVHGLKPDEHTWPITVSYGIMQRNMKGAIVSTSVFAGALTLVWASLSALTGQLLSFFQNYNLDPIVDVIVGLTMIGVALIFIIKGKDKVVDKDNNPDYKLIWIHGLAAAFGGDFIVILLLTIALSTLISVNITFLIGLLFGIGSWIAQSIVVLLIYKGLVKGVKANFQILAKAGRLSLLFLGIFMIGLGILSSLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UE9</accession>
    <name>Q97UE9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ZIP family metal transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90489">
        <property type="entry name" value="E90489"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990867.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UE9"/>
    <dbReference type="STRING" id="273057.SSO3073"/>
    <dbReference type="PaxDb" id="273057-SSO3073"/>
    <dbReference type="EnsemblBacteria" id="AAK43172">
        <property type="protein sequence ID" value="AAK43172"/>
        <property type="gene ID" value="SSO3073"/>
    </dbReference>
    <dbReference type="GeneID" id="15298676"/>
    <dbReference type="KEGG" id="sso:SSO3073"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3172"/>
    <dbReference type="eggNOG" id="arCOG00576">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094845_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UE9"/>
    <dbReference type="PhylomeDB" id="Q97UE9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071577">
        <property type="term" value="P:zinc ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="26329" checksum="721250D3586D39EA" modified="2001-10-01" version="1">MNYFPILLGLIAGGTVAIGAIIISKGKFNKTTIGYLGAFTGGILSYLALDTGSEAEEIVTNFLAVKDYPDFTVALILTSIGLIGTWLLLSLLEKNPSGEGLPVVVASALGFHNIGEGFAIAAALLSGSVAAAWAFTIGFAVHNATEGFAISSPAVMSKRVMGLKVLIPLSLLAGLPTTLGASIYYLGISNEIFLAFLNVVASASLVFVMIRININAASLLGGFKARFWTWLFIGIATTYGLEVLLDILGGGTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UI1</accession>
    <name>Q97UI1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-glucuronidase</fullName>
            <ecNumber evidence="2">3.2.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gusB</name>
        <name evidence="9" type="ordered locus">SSO3036</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.31" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90485">
        <property type="entry name" value="C90485"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990700.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UI1"/>
    <dbReference type="FunCoup" id="Q97UI1">
        <property type="interactions" value="255"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3036"/>
    <dbReference type="CAZy" id="GH2">
        <property type="family name" value="Glycoside Hydrolase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3036"/>
    <dbReference type="EnsemblBacteria" id="AAK43138">
        <property type="protein sequence ID" value="AAK43138"/>
        <property type="gene ID" value="SSO3036"/>
    </dbReference>
    <dbReference type="GeneID" id="1453057"/>
    <dbReference type="KEGG" id="sso:SSO3036"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3135"/>
    <dbReference type="eggNOG" id="arCOG07337">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006501_6_1_2"/>
    <dbReference type="InParanoid" id="Q97UI1"/>
    <dbReference type="PhylomeDB" id="Q97UI1"/>
    <dbReference type="BRENDA" id="3.2.1.31">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004566">
        <property type="term" value="F:beta-glucuronidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000243">
        <property type="entry name" value="Beta-glucuronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006101">
        <property type="entry name" value="Glyco_hydro_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006103">
        <property type="entry name" value="Glyco_hydro_2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006104">
        <property type="entry name" value="Glyco_hydro_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006102">
        <property type="entry name" value="Ig-like_GH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10066">
        <property type="entry name" value="BETA-GLUCURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10066:SF67">
        <property type="entry name" value="BETA-GLUCURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00703">
        <property type="entry name" value="Glyco_hydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02836">
        <property type="entry name" value="Glyco_hydro_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02837">
        <property type="entry name" value="Glyco_hydro_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00132">
        <property type="entry name" value="GLHYDRLASE2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="beta-Galactosidase/glucuronidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolases family 2 sugar binding" evidence="8">
        <location>
            <begin position="11"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 2 immunoglobulin-like beta-sandwich" evidence="6">
        <location>
            <begin position="166"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 2 catalytic" evidence="7">
        <location>
            <begin position="259"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK43138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="570" mass="66796" checksum="DEB2FEC8050AF189" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UJ3</accession>
    <name>Q97UJ3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90483">
        <property type="entry name" value="A90483"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924092.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UJ3"/>
    <dbReference type="STRING" id="273057.SSO3018"/>
    <dbReference type="PaxDb" id="273057-SSO3018"/>
    <dbReference type="EnsemblBacteria" id="AAK43120">
        <property type="protein sequence ID" value="AAK43120"/>
        <property type="gene ID" value="SSO3018"/>
    </dbReference>
    <dbReference type="GeneID" id="1453039"/>
    <dbReference type="KEGG" id="sso:SSO3018"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3112"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UJ3"/>
    <dbReference type="PhylomeDB" id="Q97UJ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="80"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37343" checksum="77A7D0E368408E8A" modified="2001-10-01" version="1">MQTIQVSKTELKSLAINLATININVISQDLDPEIVKAAPSLLTGNRGKYYLKVVRQGEKVISKGQRTFKFYPIYREVKGETNVVAVDETGLTVGEKEQEKAEGFLLYNWKRKGVKMRSLDLVYPLRLPLLVEVADLRSDSPSQFLLRSVREVSQYMEIDYVVADAGFLNLGVIKEMPVKTIVRGKSNLKGFKELSNVPLVEKRYEVKDKVYVAYRVLEFEGLYYYDVVYVKGKPRHFMFVTNFEGDPYELAELYRLRWQVEEGFKVRKARIRYVRKLSNKIFLFLYYTVLDSAWNLVNHLLFNFKSTCKKVLSFDSFVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UM0</accession>
    <name>Q97UM0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2983</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90479">
        <property type="entry name" value="A90479"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UM0"/>
    <dbReference type="STRING" id="273057.SSO2983"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2983"/>
    <dbReference type="DNASU" id="1453007"/>
    <dbReference type="EnsemblBacteria" id="AAK43088">
        <property type="protein sequence ID" value="AAK43088"/>
        <property type="gene ID" value="SSO2983"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2983"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3075"/>
    <dbReference type="eggNOG" id="arCOG01417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053640_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UM0"/>
    <dbReference type="PhylomeDB" id="Q97UM0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="233"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="409" mass="47494" checksum="FEAE5CA6944AC08F" modified="2001-10-01" version="1">MRKMRVLAIGNVFNPSGVSVHIINVLKELVKMGDEVFLYVPSFLVRRNYEVLKDLENAHISVHHSVYETKEAEEVGTLPYFIRSHTVYLRWNNLGEDRKYIDELKDIKPDVIYDMHEDSITLRLSYHIAKRLNKPLVKLLHDEPFRNSFGRGYRKFLGIKGLAYDTLMWMFYKFDKRAFLRSINDGILRGIAGVSKAPFYLSGIEEIAHSVRLKVYEVGNAFNKDIIYKYRRTRGKEDYAVFFARLVPQKGIKELPKIADLLDSKIVVFGKIFNENDRSILMSHPKIEYKGYRPIEEVYNTVAKAKVLIYPSHQDGFSLVVLDTLALGTSVVAYDIPAIRFVFSSLKPVRTVREYDIKEMASTANSILRMKDEEYESEHEDEKVKKFLELHSSWANVAKETHDFLSAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-10-02" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UP7</accession>
    <name>Q97UP7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1395</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90475">
        <property type="entry name" value="F90475"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UP7"/>
    <dbReference type="STRING" id="273057.SSO2957"/>
    <dbReference type="PaxDb" id="273057-SSO2957"/>
    <dbReference type="EnsemblBacteria" id="AAK43061">
        <property type="protein sequence ID" value="AAK43061"/>
        <property type="gene ID" value="SSO2957"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2957"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3048"/>
    <dbReference type="eggNOG" id="arCOG02134">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172291_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UP7"/>
    <dbReference type="PhylomeDB" id="Q97UP7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13551">
        <property type="entry name" value="HTH_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="127" mass="15437" checksum="BCECAE6DC9B020BB" modified="2001-10-01" version="1">MGVLRTLKRRIRKLIEKKPKNLNETKSRGILLYLEGMNTRKIAKILQVHLSTVYRRINQFEKEGEKCLFYKERKGRNKKMDEKEIKVEELQETIWEAKAYIEEKFHVKLSYVTAWRIARKRLKIPYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UQ2</accession>
    <name>Q97UQ2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Deoxycytidine triphosphate deaminase (DcD-2)</fullName>
            <ecNumber evidence="3">3.5.4.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dcD-2</name>
        <name evidence="3" type="ordered locus">SSO2954</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.4.13" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99475">
        <property type="entry name" value="A99475"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UQ2"/>
    <dbReference type="FunCoup" id="Q97UQ2">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2954"/>
    <dbReference type="PaxDb" id="273057-SSO2954"/>
    <dbReference type="EnsemblBacteria" id="AAK43056">
        <property type="protein sequence ID" value="AAK43056"/>
        <property type="gene ID" value="SSO2954"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2954"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3043"/>
    <dbReference type="eggNOG" id="arCOG04048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087476_3_0_2"/>
    <dbReference type="InParanoid" id="Q97UQ2"/>
    <dbReference type="PhylomeDB" id="Q97UQ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015949">
        <property type="term" value="P:nucleobase-containing small molecule interconversion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680:SF3">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22769">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="166" mass="18711" checksum="DB61648EF9447288" modified="2001-10-01" version="1">MSNLFSEMILSHQSIKKLLGKVIINYVEENVRENGYDLRICGDKYYVVEEGAQLPAIKAKIREIPFKEVADLSPLKTYLFESCEEFNMPENLAVLITLKSTMARNGFLAPPTVIDAGYKGKIYLAITSVYNSSLAKGISTHHLIFLELDQRTERIYSGKYQGGIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UQ6</accession>
    <name>Q97UQ6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2951</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90474">
        <property type="entry name" value="E90474"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924049.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UQ6"/>
    <dbReference type="STRING" id="273057.SSO2951"/>
    <dbReference type="PaxDb" id="273057-SSO2951"/>
    <dbReference type="EnsemblBacteria" id="AAK43052">
        <property type="protein sequence ID" value="AAK43052"/>
        <property type="gene ID" value="SSO2951"/>
    </dbReference>
    <dbReference type="GeneID" id="1452971"/>
    <dbReference type="KEGG" id="sso:SSO2951"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3036"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066381_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UQ6"/>
    <dbReference type="PhylomeDB" id="Q97UQ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="1">
        <location>
            <begin position="40"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="2">
        <location>
            <begin position="236"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="357" mass="41305" checksum="C2F88E92D1A0DAF5" modified="2001-10-01" version="1">MVEMGIEAKSSGEGVTSPYSRTEHCENIHEYRCDTRVGVIGIDVSKEHLVTSKGRVRKFTNNEKGYEEILTMKPNIIVIEPTGVYSTRPCQYFKERGVKVLLVSPTLLWKEKDVRGKKTDFYDAEKLENMVNKAREYVNNPLKELVSLYLFMKDVQTKQENRLRRALFLVSDEEKLSEERLEKFSKGDFSDVKLYQLEYTGTVLREIQVLARTLLQTREELKEVRKMIEGNVPQDHVLLTIPGIGKLAAGIIIGIVGDIKRFPRPESFVAYCGLDPVVERSGRIEVRKGISKRGNKYLRSLFYFLAETQYSRNPTLLKFYETHKDKLKGRKLYTALAGKLARIVWSVWYNNKPYEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UR3</accession>
    <name>Q97UR3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Maleate cis-trans isomerase, probable (MaiA)</fullName>
            <ecNumber evidence="1">5.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">maiA</name>
        <name evidence="1" type="ordered locus">SSO2939</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.2.1.1" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90473">
        <property type="entry name" value="D90473"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989870.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UR3"/>
    <dbReference type="FunCoup" id="Q97UR3">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2939"/>
    <dbReference type="PaxDb" id="273057-SSO2939"/>
    <dbReference type="EnsemblBacteria" id="AAK43043">
        <property type="protein sequence ID" value="AAK43043"/>
        <property type="gene ID" value="SSO2939"/>
    </dbReference>
    <dbReference type="GeneID" id="1452962"/>
    <dbReference type="KEGG" id="sso:SSO2939"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="PhylomeDB" id="Q97UR3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050076">
        <property type="term" value="F:maleate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053714">
        <property type="entry name" value="Iso_Racemase_Enz_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026286">
        <property type="entry name" value="MaiA/AMDase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40267">
        <property type="entry name" value="BLR3294 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40267:SF1">
        <property type="entry name" value="BLR3294 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17645">
        <property type="entry name" value="Amdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015736">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="242" mass="27682" checksum="E945F26BBF244CB7" modified="2001-10-01" version="1">MEWIKVGVIIPSSNTTVEYEFSNVFNQIKEYRITPHFSRIKLRNVTIEDLAEMERETERAASELSTILPQIISYACTTGSLFKGPKHHEEIIQRIEKIAKVPATATSGSVINALRRLDIKRLVLLTPYIEEVNKKEIEFFSKNGFEIVKSAGMGIRENIKIGQVTPEEVYNFAVNVLRNVKGDYDGVFISCTNLRTFEILSRLEKETKKPVISSNSATLWETLYKINLNIKVRELGHLFEIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-10-02" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97US8</accession>
    <name>Q97US8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1058</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="A90471"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97US8"/>
    <dbReference type="PaxDb" id="273057-SSO2916"/>
    <dbReference type="EnsemblBacteria" id="AAK43024">
        <property type="protein sequence ID" value="AAK43024"/>
        <property type="gene ID" value="SSO2916"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2916"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3006"/>
    <dbReference type="eggNOG" id="arCOG02754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102439_1_0_2"/>
    <dbReference type="InParanoid" id="Q97US8"/>
    <dbReference type="PhylomeDB" id="Q97US8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053172">
        <property type="entry name" value="Tn903_transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631:SF3">
        <property type="entry name" value="ISSOD12 TRANSPOSASE TNPA_ISSOD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13737">
        <property type="entry name" value="DDE_Tnp_1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase DDE" evidence="1">
        <location>
            <begin position="36"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="128" mass="15336" checksum="7069EBD254C273B9" modified="2001-10-01" version="1">MEKSKYKRDWSKYDENVITRYKLMFPFYVFEHWWDLLAEENRNARTKYKAPKEFNEFLAFLHIFLPYRAIEGVLRALEQLKIIPTSLDYSTIWERVRNMNITFPETSDELEVIADATGISTNTGRCPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UT1</accession>
    <name>Q97UT1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2913</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90470">
        <property type="entry name" value="F90470"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924029.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UT1"/>
    <dbReference type="STRING" id="273057.SSO2913"/>
    <dbReference type="PaxDb" id="273057-SSO2913"/>
    <dbReference type="EnsemblBacteria" id="AAK43021">
        <property type="protein sequence ID" value="AAK43021"/>
        <property type="gene ID" value="SSO2913"/>
    </dbReference>
    <dbReference type="GeneID" id="1452940"/>
    <dbReference type="KEGG" id="sso:SSO2913"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3002"/>
    <dbReference type="eggNOG" id="arCOG02617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177103_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UT1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054230">
        <property type="entry name" value="DUF6955"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22271">
        <property type="entry name" value="DUF6955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="101" mass="11826" checksum="A9ECADDDDA7F6AE0" modified="2001-10-01" version="1">MEVKIYIWLNEELSRKFEELGLKHAKEVLGGMKRIEIDVEQEMVEEIIKLFPNAKVDSSTTKSIELLPKSFKNEILKIIIEKKLEPREALREAIKKFKGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UZ8</accession>
    <name>Q97UZ8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter, permease (Branched chain amino acid)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2841</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90461">
        <property type="entry name" value="F90461"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923993.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UZ8"/>
    <dbReference type="FunCoup" id="Q97UZ8">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2841"/>
    <dbReference type="PaxDb" id="273057-SSO2841"/>
    <dbReference type="EnsemblBacteria" id="AAK42949">
        <property type="protein sequence ID" value="AAK42949"/>
        <property type="gene ID" value="SSO2841"/>
    </dbReference>
    <dbReference type="GeneID" id="1452868"/>
    <dbReference type="KEGG" id="sso:SSO2841"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2925"/>
    <dbReference type="eggNOG" id="arCOG01273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_1_0_2"/>
    <dbReference type="InParanoid" id="Q97UZ8"/>
    <dbReference type="PhylomeDB" id="Q97UZ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482:SF1">
        <property type="entry name" value="BRANCHED-CHAIN AMINO ACID TRANSPORT PERMEASE PROTEIN LIVM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="345" mass="36956" checksum="9166F6A4A76F4130" modified="2001-10-01" version="1">MVVDISSLILAILLDFSVYLMVVISLNVEIGLTGVPNFGRVLAYAGGAFIAGAVPGRILQIIYGLHGDYILNNPSIIAEINDKLLEQPLMALAILILTLALAGIVGATLGYISSYPALRLREDYLGILLLVAGTSLVVVGITYPPIVGGTVGVQVPAVFNALLGQGASLYYKISILLLVFSLIMLLLFNLISNSPMGRAFRAVRENEDLVEVLGRDIGVIRGKAMALGGFLAGVAGSLYAFYIGSVFAGAFDNLTWSFNPYLMLILGGAGTSIGALLGALIYSTLYHVIDLYKQTIGYYLHFDAVWLQYMLFGLVLTIILILRPQGIVVEKAKPLVKPKAKDQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="143" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V13</accession>
    <name>Q97V13_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Formate dehydrogenase Alpha subunit (FdhF-2)</fullName>
            <ecNumber evidence="17">1.2.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">fdhF-2</name>
        <name evidence="17" type="ordered locus">SSO2824</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.2" evidence="17"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90459">
        <property type="entry name" value="G90459"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989018.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V13"/>
    <dbReference type="FunCoup" id="Q97V13">
        <property type="interactions" value="337"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2824"/>
    <dbReference type="PaxDb" id="273057-SSO2824"/>
    <dbReference type="EnsemblBacteria" id="AAK42934">
        <property type="protein sequence ID" value="AAK42934"/>
        <property type="gene ID" value="SSO2824"/>
    </dbReference>
    <dbReference type="GeneID" id="1452853"/>
    <dbReference type="KEGG" id="sso:SSO2824"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2909"/>
    <dbReference type="eggNOG" id="arCOG01492">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_2_2_2"/>
    <dbReference type="InParanoid" id="Q97V13"/>
    <dbReference type="PhylomeDB" id="Q97V13"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008863">
        <property type="term" value="F:formate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015942">
        <property type="term" value="P:formate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02753">
        <property type="entry name" value="MopB_Formate-Dh-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.90:FF:000001">
        <property type="entry name" value="Formate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.740:FF:000003">
        <property type="entry name" value="Formate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.228.10:FF:000002">
        <property type="entry name" value="Formate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000032">
        <property type="entry name" value="Formate dehydrogenase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000005">
        <property type="entry name" value="Periplasmic nitrate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.90">
        <property type="entry name" value="ADC-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.228.10">
        <property type="entry name" value="Dimethylsulfoxide Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041924">
        <property type="entry name" value="Formate_Dh-H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006478">
        <property type="entry name" value="Formate_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01591">
        <property type="entry name" value="Fdh-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF14">
        <property type="entry name" value="FORMATE DEHYDROGENASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13510">
        <property type="entry name" value="Fer2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036643">
        <property type="entry name" value="FDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00490">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="9 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="13">
        <location>
            <begin position="4"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="16">
        <location>
            <begin position="80"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="14">
        <location>
            <begin position="137"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="14">
        <location>
            <begin position="182"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="15">
        <location>
            <begin position="257"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AAK42934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="979" mass="108800" checksum="FFB62A3608AE8B29" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V16</accession>
    <name>Q97V16_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">pyruvate, water dikinase</fullName>
            <ecNumber evidence="5">2.7.9.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Pyruvate, water dikinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ppsA-2</name>
        <name evidence="15" type="ordered locus">SSO2820</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>pyruvate + ATP + H2O = phosphoenolpyruvate + AMP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11364"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.2" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90459">
        <property type="entry name" value="D90459"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989023.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V16"/>
    <dbReference type="STRING" id="273057.SSO2820"/>
    <dbReference type="PaxDb" id="273057-SSO2820"/>
    <dbReference type="EnsemblBacteria" id="AAK42931">
        <property type="protein sequence ID" value="AAK42931"/>
        <property type="gene ID" value="SSO2820"/>
    </dbReference>
    <dbReference type="GeneID" id="1452850"/>
    <dbReference type="KEGG" id="sso:SSO2820"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2906"/>
    <dbReference type="eggNOG" id="arCOG01114">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007308_6_0_2"/>
    <dbReference type="InParanoid" id="Q97V16"/>
    <dbReference type="PhylomeDB" id="Q97V16"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008986">
        <property type="term" value="F:pyruvate, water dikinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006319">
        <property type="entry name" value="PEP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43030">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43030:SF1">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="14">
        <location>
            <begin position="16"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAK42931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="312" mass="34975" checksum="57C9DE3ACEBB929E" modified="2001-10-01" version="1">MNYTYLLDEVSLSMVSIVGRKSAYLGELYKMGFNIPKGFIISSRGVNEAIKDLDDEIRGILSSVNLNDTTDLEKRSEMIKSMIIASKLPNEMEKEIYERFSQLGSKYVAVRATATSPLSGASFAGEYETDLFVTQENLIPSIKRVIASYFNPRAIAYRILTHNEAGMAILVQTMINPVSAGTAFSIHPITEEPDYVVIESSFGLGESVTKGMVTPDQYVVSKATRSLVSKRISEKVMKLTYDFAEKKIKSIELSKEEALAESLSDNDAIRIANMAIAIESIFKRNINIEWAIEDKKVYLLEVRGIRRLYPEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V26</accession>
    <name>Q97V26_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Hydrolase, acetamydase/formamidase related, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90458">
        <property type="entry name" value="B90458"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923981.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V26"/>
    <dbReference type="FunCoup" id="Q97V26">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2810"/>
    <dbReference type="PaxDb" id="273057-SSO2810"/>
    <dbReference type="EnsemblBacteria" id="AAK42921">
        <property type="protein sequence ID" value="AAK42921"/>
        <property type="gene ID" value="SSO2810"/>
    </dbReference>
    <dbReference type="GeneID" id="1452840"/>
    <dbReference type="KEGG" id="sso:SSO2810"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2895"/>
    <dbReference type="eggNOG" id="arCOG01004">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032013_1_0_2"/>
    <dbReference type="InParanoid" id="Q97V26"/>
    <dbReference type="PhylomeDB" id="Q97V26"/>
    <dbReference type="BRENDA" id="3.5.2.B2">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.580">
        <property type="entry name" value="Acetamidase/Formamidase-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.20">
        <property type="entry name" value="Acetamidase/Formamidase-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004304">
        <property type="entry name" value="FmdA_AmdA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31891">
        <property type="entry name" value="FORMAMIDASE C869.04-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31891:SF1">
        <property type="entry name" value="FORMAMIDASE C869.04-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03069">
        <property type="entry name" value="FmdA_AmdA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141130">
        <property type="entry name" value="Acetamidase/Formamidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="318" mass="34950" checksum="A6AA73D64E7C73FA" modified="2001-10-01" version="1">MQYTIHAKTHNKWDNSLPPILTISDGDIITVETKEASDGQVTPSSTEKDLLKLDFSKIHPLTGPIEVKGAEPGDALEVEFLEFRHKGWGWTGVLPGFGFLADEQYTAPIDLQGPALKIWKVDEKYAYAKFGDLNVKVPIYPFPGVIGTGLPFRGKLSTIPPRENGGNMDIKHLTVGTKLYLPVFVNGALLSIGDTHLAQGDGEVCGTAIEAPMEVTVKVRLIKNVGLTQPLFVTKKVKEMEYDEYIAYPGIDSNLWNATKKAIKGIISILSKYMAPVEAYILASVVVNLRVSEVVDVPNWIVTAYLPKNVFDVNVEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V44</accession>
    <name>Q97V44_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2788</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90455">
        <property type="entry name" value="F90455"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991939.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V44"/>
    <dbReference type="SMR" id="Q97V44"/>
    <dbReference type="PaxDb" id="273057-SSO2788"/>
    <dbReference type="EnsemblBacteria" id="AAK42901">
        <property type="protein sequence ID" value="AAK42901"/>
        <property type="gene ID" value="SSO2788"/>
    </dbReference>
    <dbReference type="GeneID" id="1452820"/>
    <dbReference type="KEGG" id="sso:SSO2788"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2874"/>
    <dbReference type="eggNOG" id="arCOG04119">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1933356_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V44"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="15623" checksum="312114A25632CEFA" modified="2001-10-01" version="1">MEWRFEETFRKAITIIRRFYPDYALIGRYARNFYAPPETTLDIDFLVNVDDIEILAELIDYVRSQGYEIHPDDIGPWQYKIVIKGIRVDLVKPKGIDIDNEMISKRRVVELVNFISFLRRIWLFFMSFPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V59</accession>
    <name>Q97V59_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Electron transfert oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2776</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90453">
        <property type="entry name" value="G90453"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990220.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V59"/>
    <dbReference type="FunCoup" id="Q97V59">
        <property type="interactions" value="243"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2776"/>
    <dbReference type="PaxDb" id="273057-SSO2776"/>
    <dbReference type="EnsemblBacteria" id="AAK42886">
        <property type="protein sequence ID" value="AAK42886"/>
        <property type="gene ID" value="SSO2776"/>
    </dbReference>
    <dbReference type="GeneID" id="1452805"/>
    <dbReference type="KEGG" id="sso:SSO2776"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2858"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050977_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V59"/>
    <dbReference type="PhylomeDB" id="Q97V59"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039651">
        <property type="entry name" value="FixC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43624">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-QUINONE OXIDOREDUCTASE YDIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43624:SF2">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-QUINONE OXIDOREDUCTASE YDIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12831">
        <property type="entry name" value="FAD_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="407" mass="45243" checksum="0146268D88FFC2C8" modified="2001-10-01" version="1">MSFDADVIIVGGGLAGLSAAITANREGLSTIVLERGEYSGSKNVSGGRMYTHALLQLFPDALERAPLERPVTNETYEIYCEEEKKITFSFRHKTRNSYTVLRAKFDQWLAKEAENEGVLISYSTLVTNARRENDYVVIETNRGELKAPLVIDAGGITAPVSRFLGVKKLEPKTLMLGVKEVLDIKPDMDDDEGEARTIVGLIGNLKGGGFVYTNKDTLSVGVTIKVESLYNSNIPSHEIVERFRESLGLSGSILEYSAHLIPYYGYDKIGKIYDKNLILVGDAAGFLINDGFNIRGMDLAMGSGIIAGKSAKKIKELNDYSRTDIYYEMLKESFVLKDMKTALRSFSVLNEEEIFTKYPKVICNVLGQLFTVTSDGKERPINVLIKESKSENVNTTKMLTDIMRLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V80</accession>
    <name>Q97V80_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90451">
        <property type="entry name" value="B90451"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990256.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V80"/>
    <dbReference type="STRING" id="273057.SSO2755"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2755"/>
    <dbReference type="EnsemblBacteria" id="AAK42865">
        <property type="protein sequence ID" value="AAK42865"/>
        <property type="gene ID" value="SSO2755"/>
    </dbReference>
    <dbReference type="GeneID" id="1452784"/>
    <dbReference type="KEGG" id="sso:SSO2755"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2834"/>
    <dbReference type="eggNOG" id="arCOG01408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_616268_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V80"/>
    <dbReference type="PhylomeDB" id="Q97V80"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="242"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="437" mass="48855" checksum="D3F60271C39249CA" modified="2001-10-01" version="1">MISVAINTQTPPIRFSLTYRDLLEKYGYLEPPIDLNLLSNDDYHISVGGVAKMMLSLIKTRAFSKSRWVSLGPGYPPSVKMEEAEVYFVDLDAKSLANYTRFKEGLYNESHGLSKYNLVGEEYIAYANYNWLSAQKLLEFYNDTDIYFINDFQQLLVGGIIGPSAPAVLWYHIPFVPENLSRKLKEFLIKAFESFDLVILSTKRDLEGLVRTGAKVKVKQIYPFIDPSSYKTVGRKEIQYIEDKYQLKSDDKVILVVARMDPMKSQDLAISAIKNVDAKLVLAGDGSFTSKTLGHDKGSMWVSRLKELARSLGVENKVVFTGYVPDEELFALYQRANVVLLPSRIEGFGLAVCEGWVYGKPAVVSSGAGISELIIEGGNGFVFKSGNIDELAEKLKLALKDEKVGSLGKETVKKCSVSNALNELKEGFEIASEEYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VA8</accession>
    <name>Q97VA8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2726</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90447">
        <property type="entry name" value="F90447"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924066.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VA8"/>
    <dbReference type="STRING" id="273057.SSO2726"/>
    <dbReference type="PaxDb" id="273057-SSO2726"/>
    <dbReference type="EnsemblBacteria" id="AAK42837">
        <property type="protein sequence ID" value="AAK42837"/>
        <property type="gene ID" value="SSO2726"/>
    </dbReference>
    <dbReference type="GeneID" id="1452756"/>
    <dbReference type="KEGG" id="sso:SSO2726"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2806"/>
    <dbReference type="eggNOG" id="arCOG03462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034270_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VA8"/>
    <dbReference type="PhylomeDB" id="Q97VA8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF7">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="490"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="529" mass="58891" checksum="FB146A90F6040585" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VB1</accession>
    <name>Q97VB1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2723</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90447">
        <property type="entry name" value="B90447"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VB1"/>
    <dbReference type="STRING" id="273057.SSO2723"/>
    <dbReference type="PaxDb" id="273057-SSO2723"/>
    <dbReference type="EnsemblBacteria" id="AAK42833">
        <property type="protein sequence ID" value="AAK42833"/>
        <property type="gene ID" value="SSO2723"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2723"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987729_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VB1"/>
    <dbReference type="PhylomeDB" id="Q97VB1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="125" mass="14363" checksum="94768E91060AB2B0" modified="2001-10-01" version="1">MVDLVTEAKSSGEGVTSPYSRTEHCENIHEYRCDTRVGVIGIDVSKEHLVTSKGRVRKFTNNEKGYEEILTMKPNIIVIEPTGVYSTRPCQYFKERGVKVLLVSLAMERKGRKGKENRFLRRRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="148" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VD9</accession>
    <name>Q97VD9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mercuric reductase</fullName>
            <ecNumber evidence="4">1.16.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Hg(II) reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">merA</name>
        <name evidence="20" type="ordered locus">SSO2689</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Hg + NADP(+) + H(+) = Hg(2+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:23856"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16170"/>
            <dbReference type="ChEBI" id="CHEBI:16793"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.1" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90443">
        <property type="entry name" value="F90443"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988613.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VD9"/>
    <dbReference type="FunCoup" id="Q97VD9">
        <property type="interactions" value="224"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2689"/>
    <dbReference type="PaxDb" id="273057-SSO2689"/>
    <dbReference type="EnsemblBacteria" id="AAK42805">
        <property type="protein sequence ID" value="AAK42805"/>
        <property type="gene ID" value="SSO2689"/>
    </dbReference>
    <dbReference type="GeneID" id="1452724"/>
    <dbReference type="GeneID" id="44128417"/>
    <dbReference type="KEGG" id="sso:SSO2689"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2771"/>
    <dbReference type="eggNOG" id="arCOG01068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_1_1_2"/>
    <dbReference type="InParanoid" id="Q97VD9"/>
    <dbReference type="PhylomeDB" id="Q97VD9"/>
    <dbReference type="BRENDA" id="1.16.1.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016152">
        <property type="term" value="F:mercury (II) reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045340">
        <property type="term" value="F:mercury ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016668">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050787">
        <property type="term" value="P:detoxification of mercury ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000001">
        <property type="entry name" value="Dihydrolipoyl dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021179">
        <property type="entry name" value="Mercury_reductase_MerA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02053">
        <property type="entry name" value="MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43014">
        <property type="entry name" value="MERCURIC REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43014:SF4">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE RCLA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 17" id="KW-0274">FAD</keyword>
    <keyword evidence="7 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0475">Mercuric resistance</keyword>
    <keyword evidence="11" id="KW-0476">Mercury</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="12 17 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14 17" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="19">
        <location>
            <begin position="3"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="18">
        <location>
            <begin position="330"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AAK42805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="453" mass="49415" checksum="1FCF004D65982047" modified="2001-10-01" version="1">MEDLVIIGYGAAGFAALIRANELGIKPVVVGYGEIGGTCVNVGCVPSKRMLRIGELYNYSSKVIGKKLFPEFFQAFQDKAEIVNSLRKEKYEDVINSYDIKLKIGKAYFTSPNAVKVNGEIIEAKKFIIATGSSPNIPNIKGLTEVGFWTNVEALSPDKTISSLAIIGGRALALEFAQMYKRLGVDTTILQRSGRILPDWEPEISLSVKNYLEENDSIPIFTNVRVKEVRKGNGGKIIVTDKGEVEADEILLATGRKPNVDLNLDAAGIELNDKGGIKVNEELRTSNPNVFAAGDVIGGPMLEALAGRQGSIAAENAIMNVHRKIDMLSVPQVVFIEPNVAKVGLTALEAVKEGYDIDQRVVKMDNIAKARILRENYGLIKMVIDKKFRNILGVQMFGKYAAEVINEAALALRFRATIDDLIDTIHVFPTMGESLRIAALAFTGDVSKMSCCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VK8</accession>
    <name>Q97VK8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Dipeptide ABC transporter ATP binding protein (DppD-3)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dppD-3</name>
        <name evidence="10" type="ordered locus">SSO2616</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99435">
        <property type="entry name" value="A99435"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VK8"/>
    <dbReference type="FunCoup" id="Q97VK8">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2616"/>
    <dbReference type="PaxDb" id="273057-SSO2616"/>
    <dbReference type="EnsemblBacteria" id="AAK42736">
        <property type="protein sequence ID" value="AAK42736"/>
        <property type="gene ID" value="SSO2616"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2616"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2697"/>
    <dbReference type="eggNOG" id="arCOG00181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="InParanoid" id="Q97VK8"/>
    <dbReference type="PhylomeDB" id="Q97VK8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000016">
        <property type="entry name" value="Oligopeptide ABC transporter ATP-binding component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050388">
        <property type="entry name" value="ABC_Ni/Peptide_Import"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43297:SF14">
        <property type="entry name" value="ATPASE AAA-TYPE CORE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43297">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT ATP-BINDING PROTEIN APPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="9"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="324" mass="36331" checksum="FB1CF6C74C0164AA" modified="2001-10-01" version="1">MNNMVVLQIRNLNVYYNTLISWVKVLSDVNLDVEKGEIVGVVGESGSGKSTLGHAISRILPPNARIQGDIIVDGVNLAKLKDSELQKYRGTWVFMIFQNPLNSLNPVKKVGSQLLEAVKIRYQREGKKAEEESLMRDVIEVLKDLRLPDPYSIIDRYPHQLSGGQVQRIVISMALLLKPKLLIADEPTSALDVTIQAQVVNLFKQLNKEINTSILFITHDISLAYVVSDRIVVMYAGRIMEDGKVEEVLKSPMHPYTQGLVASIPSGDKNQKLTAIPGNPPSFFALPTGCKFSNRCSKVFDICRKKEPSIIEKNGRKIRCWLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VT3</accession>
    <name>Q97VT3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prolidase (Xaa-Pro dipeptidase) (PepQ-like3)</fullName>
            <ecNumber evidence="2">3.4.13.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pepQ-like3</name>
        <name evidence="2" type="ordered locus">SSO2527</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.13.9" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90425">
        <property type="entry name" value="B90425"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990971.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VT3"/>
    <dbReference type="FunCoup" id="Q97VT3">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2527"/>
    <dbReference type="PaxDb" id="273057-SSO2527"/>
    <dbReference type="EnsemblBacteria" id="AAK42657">
        <property type="protein sequence ID" value="AAK42657"/>
        <property type="gene ID" value="SSO2527"/>
    </dbReference>
    <dbReference type="GeneID" id="1453984"/>
    <dbReference type="KEGG" id="sso:SSO2527"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2603"/>
    <dbReference type="eggNOG" id="arCOG00696">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023620_2_2_2"/>
    <dbReference type="InParanoid" id="Q97VT3"/>
    <dbReference type="PhylomeDB" id="Q97VT3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102009">
        <property type="term" value="F:proline dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01299">
        <property type="entry name" value="Met_dep_hydrolase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051781">
        <property type="entry name" value="Metallo-dep_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057744">
        <property type="entry name" value="OTAase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135:SF3">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="49"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="407" mass="44553" checksum="8F1A4C18ED297BF3" modified="2001-10-01" version="1">MDLGIKNGNVFNGKGVVGKVNIYVKDGKIVEISKENKQAKIEIDAKDMFVMPGLIDAHIHLTGIKGGSLLKTMFEKPEYRVLRASKWLEKLLLAGFTTVRDCGETISLALKRGVSEGIINGPRIIAAGKPISQTFGHGELSHDVPLEFSMALSFSEFCDGVESCIRASRKVLRDGADFIKIFATGGVLSQRDRPEHPQLSYEEISAIVNEAEKINTYVAAHAHGDKGARIAVEAGIKTLEHGTLLRDETLKLMRERNVTLTPTLTIQELIYKYGKQIGVDEWGLQKINEVRESVVNVVKKAEEYGVMIITGTDLGFETGLEEIDIGKNWMESVLLVERGGLSTISSLRASTYNSAFAIGINAGAIEVGKDADIIVIDGDPTENIRDLSKVRRVVKNGKLTVEDGKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VX2</accession>
    <name>Q97VX2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate--CoA ligase [ADP-forming] subunit alpha</fullName>
            <ecNumber evidence="4">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="4">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 9" type="primary">sucD</name>
        <name evidence="9" type="ordered locus">SSO2482</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 7">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>GTP + succinate + CoA = succinyl-CoA + GDP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22120"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 7">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 7">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 6">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C99420">
        <property type="entry name" value="C99420"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VX2"/>
    <dbReference type="FunCoup" id="Q97VX2">
        <property type="interactions" value="372"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2482"/>
    <dbReference type="PaxDb" id="273057-SSO2482"/>
    <dbReference type="EnsemblBacteria" id="AAK42618">
        <property type="protein sequence ID" value="AAK42618"/>
        <property type="gene ID" value="SSO2482"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2482"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2563"/>
    <dbReference type="eggNOG" id="arCOG01339">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052104_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VX2"/>
    <dbReference type="PhylomeDB" id="Q97VX2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
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    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004230">
        <property type="entry name" value="PRK05678.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4 7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA-binding" evidence="8">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="4 5">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="69"/>
            <end position="71"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="260" mass="27733" checksum="0E146E845FB2FC01" modified="2001-10-01" version="1">MLKYGTKIVAGVTPGKGGTQVNSVPVYDTVKDAMKEHEADASIIFVPARYAVDAIYEAVDAGIKLIVTITEHIPVLDMARAIKYARARGARIIGPNCPGIIAPEESLVGILPARAFKKGKIGIVSRSGTLTYEVSELLKNSGMGQSTVIGIGGDPIIGTSTLEVAKMFDQDPETEKIVVIGEIGGTMEERLAEAYKRGEIKKPIIAYIAGMTAPREKRMGHAGAVVYMGMGTFESKIRAFKEAGIPIANTPYDIPKLLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="137" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W22</accession>
    <name>Q97W22_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Purine phosphoribosyltransferase (GpT-2)</fullName>
            <ecNumber evidence="4">2.4.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gpT-2</name>
        <name evidence="4" type="ordered locus">SSO2424</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2015-04" db="PDB data bank">
            <title>Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Christoffersen S."/>
                <person name="Hansen M.R."/>
                <person name="Jensen K.S."/>
                <person name="Larsen S."/>
                <person name="Jensen K.F."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH GMP</scope>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="submission" date="2015-05" db="PDB data bank">
            <title>Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Christoffersen S."/>
                <person name="Hansen M.R."/>
                <person name="Jensen K.S."/>
                <person name="Larsen S."/>
                <person name="Jensen K.F."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH GMP</scope>
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    <dbReference type="EC" id="2.4.2.-" evidence="4"/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="A99414"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="4ZFN">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A/B=1-179"/>
    </dbReference>
    <dbReference type="PDB" id="5BQO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.39 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-179"/>
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    <dbReference type="PDB" id="5BQP">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A/B/C/D=1-179"/>
    </dbReference>
    <dbReference type="PDBsum" id="4ZFN"/>
    <dbReference type="PDBsum" id="5BQO"/>
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    <dbReference type="SMR" id="Q97W22"/>
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    </dbReference>
    <dbReference type="STRING" id="273057.SSO2424"/>
    <dbReference type="PaxDb" id="273057-SSO2424"/>
    <dbReference type="EnsemblBacteria" id="AAK42568">
        <property type="protein sequence ID" value="AAK42568"/>
        <property type="gene ID" value="SSO2424"/>
    </dbReference>
    <dbReference type="GeneID" id="1453895"/>
    <dbReference type="KEGG" id="sso:SSO2424"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2502"/>
    <dbReference type="eggNOG" id="arCOG00040">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080904_1_2_2"/>
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    <dbReference type="PhylomeDB" id="Q97W22"/>
    <dbReference type="BRENDA" id="2.4.2.8">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97W22"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF1">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="6 7 8" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="3">
        <location>
            <begin position="8"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="4ZFN"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="5BQO"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="5BQP"/>
        </source>
    </evidence>
    <sequence length="179" mass="20656" checksum="232F64D3C39025B4" modified="2001-10-01" version="1">MVEYHIPSWDEIEDAVFSIGEALVKSNYIPDVLIAVLTGGIIPAKLLSDLLDLKVIRYIDIKFYRSVGKTESKPVIRSVYTDSLEGKKVLVVDDVADTGETLEAVSNVITMFNPAKVMTAALYLKPWSKRIPDFYYKQIDKWIIFPWDKWDVVRENSNVPVDKKERFLNLYNQLLKIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W75</accession>
    <name>Q97W75_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90407">
        <property type="entry name" value="B90407"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989488.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W75"/>
    <dbReference type="STRING" id="273057.SSO2360"/>
    <dbReference type="PaxDb" id="273057-SSO2360"/>
    <dbReference type="EnsemblBacteria" id="AAK42513">
        <property type="protein sequence ID" value="AAK42513"/>
        <property type="gene ID" value="SSO2360"/>
    </dbReference>
    <dbReference type="GeneID" id="1453840"/>
    <dbReference type="KEGG" id="sso:SSO2360"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2447"/>
    <dbReference type="eggNOG" id="arCOG00442">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_598029_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W75"/>
    <dbReference type="PhylomeDB" id="Q97W75"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36846">
        <property type="entry name" value="PROTEIN VIAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36846:SF1">
        <property type="entry name" value="PROTEIN VIAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="289"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="452" mass="51116" checksum="4E57E68868A5AC67" modified="2001-10-01" version="1">MSEEEGVLRGIDYRDPLVKYRGERISYTLKKLLGRDVQLNETFLVDTYYVHYLPLPITKGKSEIEKNQEIAYSLVNSTLSSDIVLKNREYSIVNSAVSLALTVSYVQNLIEELERIKKTSQSMEEREAAEEILNGLMKGSSSKEGKEQKNSNQQSMEKVLRQAHEKAMSKAIEDANSVRNMQKIVGGNGAGTGSVLTFEGEIHEVLRLARNTEIKKILEFLSGIPKLGSITKRRTTRFSKGELYGYEEGSDIERIVYSELALPDMLFYLKLAEGQLLLYQKQIKETLGPIYLLLDKSGSMDGEKILWAKAVALALYSRAKRENRDFYLRFFDNIPYPLIKVQKNAKSKDVIKMIEYIGKIRGGGGTDISRSIISACEDIKEGHVKGVSEIILLTDGEDKIAETTVRRSLKEANSQLISVMIRGDNADLRRVSDEYLITYKLDHEDLLKVVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WH9</accession>
    <name>Q97WH9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2238</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90393">
        <property type="entry name" value="H90393"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992032.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WH9"/>
    <dbReference type="STRING" id="273057.SSO2238"/>
    <dbReference type="PaxDb" id="273057-SSO2238"/>
    <dbReference type="EnsemblBacteria" id="AAK42407">
        <property type="protein sequence ID" value="AAK42407"/>
        <property type="gene ID" value="SSO2238"/>
    </dbReference>
    <dbReference type="GeneID" id="1453734"/>
    <dbReference type="KEGG" id="sso:SSO2238"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2334"/>
    <dbReference type="eggNOG" id="arCOG05886">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2127942_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WH9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="108" mass="12213" checksum="D6A9CF15F0E3F6EB" modified="2001-10-01" version="1">MIIFYAIGERERAKELVRIITKTRWKTISKHAIKIASSSIGPSVVIFKPTMAGLAVALWLKQRAEELGMTSAVGWFQPINQIPPQVEDAIRTDLNKILMKKLEIPWSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WN7</accession>
    <name>Q97WN7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription regulator, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO9953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90386">
        <property type="entry name" value="F90386"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992127.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WN7"/>
    <dbReference type="STRING" id="273057.SSO9953"/>
    <dbReference type="PaxDb" id="273057-SSO9953"/>
    <dbReference type="EnsemblBacteria" id="AAK42349">
        <property type="protein sequence ID" value="AAK42349"/>
        <property type="gene ID" value="SSO9953"/>
    </dbReference>
    <dbReference type="GeneID" id="1453676"/>
    <dbReference type="KEGG" id="sso:SSO9953"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2270"/>
    <dbReference type="eggNOG" id="arCOG00731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2565816_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WN7"/>
    <dbReference type="PhylomeDB" id="Q97WN7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38600">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38600:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="89" mass="10845" checksum="E547FCCAB03D766C" modified="2001-10-01" version="1">MLLRLDEIFQNKGWDTRKKILNELEEKPQTAYELSKKLGLNYSTVKYHLEILEKFGLVNINRDKNKCFYTVTKNYKIVEKYLEEEIRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WR3</accession>
    <name>Q97WR3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Catechol 2,3-dioxygenase (Metapyrocatechase)</fullName>
            <ecNumber evidence="4">1.13.11.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2054</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.13.11.2" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90372">
        <property type="entry name" value="H90372"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WR3"/>
    <dbReference type="FunCoup" id="Q97WR3">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2054"/>
    <dbReference type="PaxDb" id="273057-SSO2054"/>
    <dbReference type="EnsemblBacteria" id="AAK42239">
        <property type="protein sequence ID" value="AAK42239"/>
        <property type="gene ID" value="SSO2054"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2054"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2134"/>
    <dbReference type="eggNOG" id="arCOG06037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052361_3_0_2"/>
    <dbReference type="InParanoid" id="Q97WR3"/>
    <dbReference type="PhylomeDB" id="Q97WR3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018577">
        <property type="term" value="F:catechol 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011981">
        <property type="entry name" value="DHPA_dOase_Mn/Fe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051332">
        <property type="entry name" value="Fosfomycin_Res_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018146">
        <property type="entry name" value="Glyoxalase_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02295">
        <property type="entry name" value="HpaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36113:SF6">
        <property type="entry name" value="FOSFOMYCIN RESISTANCE PROTEIN FOSX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36113">
        <property type="entry name" value="LYASE, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00934">
        <property type="entry name" value="GLYOXALASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="3">
        <location>
            <begin position="7"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="3">
        <location>
            <begin position="139"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="316" mass="37031" checksum="24E2E2EE777C2D02" modified="2001-10-01" version="1">MMINTLRTSHVCIRVSDLDKARYFYSELLGFVETEKDGDYIYLRGIEEGQHHSLILKKANSAGLSYIGFRVSKGEELDKAREELENLGHKVRKFREKGINEALLFESPEGIPILLYYDMEYVGDLRLAFHRHRGVSPVRLAHVNYMVKDLERELEFFKRFMGYYETERFLDKDGNLAVVWLTRRGDSHEIAIAKSSKNVPGFHHETYYVHDVKDVIKAPDILASVGLWDSIERGPGRHGVTEGYYIYLRDFDKNRIEFFTNDYVVLDPDKWKPIVWTYDQLRYRSDFWGRPIPQSWLTEWVAVEDISTGQLKGWSG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-12-21" modified="2025-06-18" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WT4</accession>
    <name>Y2030_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Putative ABC transporter ATP-binding protein SSO2030</fullName>
            <ecNumber>7.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2030</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90370">
        <property type="entry name" value="B90370"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923738.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WT4"/>
    <dbReference type="SMR" id="Q97WT4"/>
    <dbReference type="FunCoup" id="Q97WT4">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2030"/>
    <dbReference type="PaxDb" id="273057-SSO2030"/>
    <dbReference type="EnsemblBacteria" id="AAK42217">
        <property type="protein sequence ID" value="AAK42217"/>
        <property type="gene ID" value="SSO2030"/>
    </dbReference>
    <dbReference type="GeneID" id="1453544"/>
    <dbReference type="KEGG" id="sso:SSO2030"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2111"/>
    <dbReference type="eggNOG" id="arCOG00188">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_86_7_2"/>
    <dbReference type="InParanoid" id="Q97WT4"/>
    <dbReference type="PhylomeDB" id="Q97WT4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003633">
        <property type="entry name" value="Cobalt ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003642">
        <property type="entry name" value="Cobalt ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050095">
        <property type="entry name" value="ECF_ABC_transporter_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553:SF23">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553">
        <property type="entry name" value="HEAVY METAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000092162" description="Putative ABC transporter ATP-binding protein SSO2030">
        <location>
            <begin position="1"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter 1" evidence="2">
        <location>
            <begin position="6"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter 2" evidence="2">
        <location>
            <begin position="282"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="314"/>
            <end position="321"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="530" mass="60110" checksum="6F365CE6E60A57FE" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WV0</accession>
    <name>Q97WV0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nitric oxide response protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99368">
        <property type="entry name" value="A99368"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WV0"/>
    <dbReference type="STRING" id="273057.SSO2014"/>
    <dbReference type="PaxDb" id="273057-SSO2014"/>
    <dbReference type="EnsemblBacteria" id="AAK42200">
        <property type="protein sequence ID" value="AAK42200"/>
        <property type="gene ID" value="SSO2014"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2014"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2087"/>
    <dbReference type="eggNOG" id="arCOG05707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_777599_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WV0"/>
    <dbReference type="PhylomeDB" id="Q97WV0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="34324" checksum="5A82AF9C20876F82" modified="2001-10-01" version="1">MNQRFIMIYGFFGALIGNEVLVALSVEWSGKTTDNRLIAVYLSSAILGSISFLFLSQQLGLISILLSMGILLYHSKEYLGVSKIGLSPTTYNWLLFYSIMISSAIVAFQLGLGCTIPYINLIFPASMILAVMDRDIALVTGVKIARHWENVLAFILLAIGMGIYPNGSILMILAWILSFHASGLYRFKGRRYPILHLGIAWIYLLIASILVFSYDIYIHAIAVGFLFNTVFGVDVVMMDLFVNAFNRRIHVKPSYIPFILLNVGLTMRFLYNFGLSIPILLLASPLQGIGILSFFVLTLKQVVRLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WV7</accession>
    <name>Q97WV7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90367">
        <property type="entry name" value="B90367"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992201.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WV7"/>
    <dbReference type="STRING" id="273057.SSO2005"/>
    <dbReference type="PaxDb" id="273057-SSO2005"/>
    <dbReference type="EnsemblBacteria" id="AAK42193">
        <property type="protein sequence ID" value="AAK42193"/>
        <property type="gene ID" value="SSO2005"/>
    </dbReference>
    <dbReference type="GeneID" id="1453520"/>
    <dbReference type="KEGG" id="sso:SSO2005"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2080"/>
    <dbReference type="eggNOG" id="arCOG03617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2191148_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WV7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010154">
        <property type="entry name" value="CRISPR-assoc_Cas7/Cst2/DevR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052681">
        <property type="entry name" value="CRISPR-Cas7/Cst2/DevR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37459">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37459:SF1">
        <property type="entry name" value="CRISPR-ASSOCIATED PROTEIN CAS7_CST2_DEVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01905">
        <property type="entry name" value="DevR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="108" mass="11878" checksum="129A7290E2688D68" modified="2001-10-01" version="1">MEILVYKLFGNTNTTARIVVEIRVYDIPVLTGNSLKHWHSVYLAEVYITLGGKHANELCKAGIGLRGYKLESTLDDTKAATSENEAIENVCNDIHGFLITIKGQGKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WZ6</accession>
    <name>Q97WZ6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">First ORF in transposon ISC1913</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1964</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90362">
        <property type="entry name" value="D90362"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WZ6"/>
    <dbReference type="STRING" id="273057.SSO1964"/>
    <dbReference type="PaxDb" id="273057-SSO1964"/>
    <dbReference type="EnsemblBacteria" id="AAK42155">
        <property type="protein sequence ID" value="AAK42155"/>
        <property type="gene ID" value="SSO1964"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1964"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2038"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_2_0_2"/>
    <dbReference type="InParanoid" id="Q97WZ6"/>
    <dbReference type="PhylomeDB" id="Q97WZ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04762">
        <property type="entry name" value="HTH_MerR-trunc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1660.10:FF:000012">
        <property type="entry name" value="DNA-binding protein, excisionase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="4">
        <location>
            <begin position="58"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="132" mass="15426" checksum="E52C08D327646B7A" modified="2001-10-01" version="1">MERLLRPKEACQLLSISYSTLLRWIREGKIKVVTTEGGKYRIPYSEIKKYLEKREETRAVIYARVSSADQREDLERQINYLTNYATAKGYKVVEVLKDIASGLNTQRKGLLKLFKLVEGSFRYKNLSMKNRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X40</accession>
    <name>Q97X40_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">First ORF in transposon ISC1913</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1915</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90356">
        <property type="entry name" value="E90356"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923649.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X40"/>
    <dbReference type="STRING" id="273057.SSO1915"/>
    <dbReference type="PaxDb" id="273057-SSO1915"/>
    <dbReference type="EnsemblBacteria" id="AAK42108">
        <property type="protein sequence ID" value="AAK42108"/>
        <property type="gene ID" value="SSO1915"/>
    </dbReference>
    <dbReference type="GeneID" id="1453435"/>
    <dbReference type="KEGG" id="sso:SSO1915"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1979"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="InParanoid" id="Q97X40"/>
    <dbReference type="PhylomeDB" id="Q97X40"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04762">
        <property type="entry name" value="HTH_MerR-trunc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1660.10:FF:000012">
        <property type="entry name" value="DNA-binding protein, excisionase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1390:FF:000002">
        <property type="entry name" value="ORF1 in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="4">
        <location>
            <begin position="58"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="213" mass="24458" checksum="3D4BDBF73D724849" modified="2001-10-01" version="1">MERLLRPKEACQLLGISYSTLLRWIREGKIRVVTTEGGKYRIPYSEIKKYLEKREETRAVIYARVSSTDQREDLERQINYLTNYATAKGYKVVEVLKDIASGLNTQRKGLLKLFKLVEGRSVDIVLITYKDRLTRFGFEYIEEFFSTMGVKIEVVFGEEPKDDAQELVEDLISIITSFAGKIYGMRSHKKTLLVQGVKKLIGELSGEDSEVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XA7</accession>
    <name>Q97XA7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Coenzyme PQQ synthesis protein E (PqqE-4)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pqqE-4</name>
        <name evidence="8" type="ordered locus">SSO1839</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90347">
        <property type="entry name" value="D90347"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923620.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XA7"/>
    <dbReference type="FunCoup" id="Q97XA7">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1839"/>
    <dbReference type="PaxDb" id="273057-SSO1839"/>
    <dbReference type="DNASU" id="1453362"/>
    <dbReference type="EnsemblBacteria" id="AAK42035">
        <property type="protein sequence ID" value="AAK42035"/>
        <property type="gene ID" value="SSO1839"/>
    </dbReference>
    <dbReference type="GeneID" id="1453362"/>
    <dbReference type="KEGG" id="sso:SSO1839"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1899"/>
    <dbReference type="eggNOG" id="arCOG00938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_0_2"/>
    <dbReference type="InParanoid" id="Q97XA7"/>
    <dbReference type="PhylomeDB" id="Q97XA7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21123">
        <property type="entry name" value="SPASM_MftC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000325">
        <property type="entry name" value="Radical SAM domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034391">
        <property type="entry name" value="AdoMet-like_SPASM_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017200">
        <property type="entry name" value="PqqE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF7">
        <property type="entry name" value="PQQA PEPTIDE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037420">
        <property type="entry name" value="PQQ_syn_pqqE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01387">
        <property type="entry name" value="BtrN-like_SPASM_domain_contain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="32"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="378" mass="43029" checksum="8274772E937D9081" modified="2001-10-01" version="1">MIPISVLVTNAGTVSFHIKGEYNRDRPSKFSDVFRPVVTWNLTRKCNLKCLHCYINASPEGEDGLTTEEALGLIDEMAEMKIPLIIMSGGEPLMRRDFFELASYARSKGIKLALSTNGTLISESVAKRLKELDFSYVGISLDSYDPEFHDKFRGLNGAFNMTVKGIGNAINVGLNVGLRFTITAKNIHQVDEYMKLALKLGVKRITFYHLSASGRGRELREWMYTLEEYVSFINKMIDYAIKLSGKIEIETTLGQFDGVYIAKKLARNEEELEKYLKFVENSGGCGRKMISIYPNGDVYPCQFIDFYKLGNVREKPLKEIIKNIPDLFINTDKYLKGRYCDKCEYKSACKGGDRARAYYWNEDIYGDDPLCPLKTLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XB3</accession>
    <name>Q97XB3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1832</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90346">
        <property type="entry name" value="E90346"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XB3"/>
    <dbReference type="PaxDb" id="273057-SSO1832"/>
    <dbReference type="EnsemblBacteria" id="AAK42028">
        <property type="protein sequence ID" value="AAK42028"/>
        <property type="gene ID" value="SSO1832"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1832"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1892"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074024_2_0_2"/>
    <dbReference type="InParanoid" id="Q97XB3"/>
    <dbReference type="PhylomeDB" id="Q97XB3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="122" mass="14725" checksum="6566B3E74F016FE2" modified="2001-10-01" version="1">MLIVKKRKKIVVYFARATNLDLPKREVLKLYNKVRSPIETSYRNIKAFLPFTSSTKFIFRELIFVLAMIFYSLYTVFKNGMTREEFRLLLILCFLDDLSDLKDFIFNFEETLINTIDLFLWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XB5</accession>
    <name>Q97XB5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">First ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1830</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2010-01" db="PDB data bank">
            <title>The Crystal Structure of a Serine Recombinase from Sulfolobus solfataricus to 2.3A.</title>
            <authorList>
                <person name="Stein A.J."/>
                <person name="Osipiuk J."/>
                <person name="Marshall N."/>
                <person name="Bearden J."/>
                <person name="Davidoff J."/>
                <person name="Joachimiak A."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 53-192</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90346">
        <property type="entry name" value="C90346"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923616.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3LHF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B/C/D=53-192"/>
    </dbReference>
    <dbReference type="PDBsum" id="3LHF"/>
    <dbReference type="AlphaFoldDB" id="Q97XB5"/>
    <dbReference type="SMR" id="Q97XB5"/>
    <dbReference type="STRING" id="273057.SSO1830"/>
    <dbReference type="PaxDb" id="273057-SSO1830"/>
    <dbReference type="DNASU" id="1453353"/>
    <dbReference type="EnsemblBacteria" id="AAK42026">
        <property type="protein sequence ID" value="AAK42026"/>
        <property type="gene ID" value="SSO1830"/>
    </dbReference>
    <dbReference type="GeneID" id="1453353"/>
    <dbReference type="KEGG" id="sso:SSO1830"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1890"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="InParanoid" id="Q97XB5"/>
    <dbReference type="PhylomeDB" id="Q97XB5"/>
    <dbReference type="EvolutionaryTrace" id="Q97XB5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04762">
        <property type="entry name" value="HTH_MerR-trunc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1660.10:FF:000013">
        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2170:FF:000002">
        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1390:FF:000002">
        <property type="entry name" value="ORF1 in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="8" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="5">
        <location>
            <begin position="55"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="8">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="3LHF"/>
        </source>
    </evidence>
    <sequence length="192" mass="22521" checksum="069354828FD2BF2F" modified="2001-10-01" version="1">MLRPKEACQRLGISYATLREYVKKGYIKPVILQSGKWRFREEDVERLMGIIRKRKVILYARVSSSTQKDDLVNQVKYLEEQVKEYDQVITDIGSKLNMKRKGFLKLLRMILNNEVSRVVVAYPDRLVRFGFEILEEVCKAHGCEIVVINQEDKEEELVEDLVSILVSFSGKLYGMRSHKYEKVKKCVEELKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XH6</accession>
    <name>Q97XH6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90337">
        <property type="entry name" value="G90337"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989075.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XH6"/>
    <dbReference type="STRING" id="273057.SSO1765"/>
    <dbReference type="PaxDb" id="273057-SSO1765"/>
    <dbReference type="EnsemblBacteria" id="AAK41958">
        <property type="protein sequence ID" value="AAK41958"/>
        <property type="gene ID" value="SSO1765"/>
    </dbReference>
    <dbReference type="GeneID" id="1454737"/>
    <dbReference type="KEGG" id="sso:SSO1765"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1812"/>
    <dbReference type="eggNOG" id="arCOG06013">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1773285_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XH6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="146" mass="16953" checksum="77794A8649A99C21" modified="2001-10-01" version="1">MITPQQIREEEEAKKKLGIAKTIELPIGGSMFYFDIPDNPMVYVSEISGIIYINGSSYWEPELLMLKDLTKEFVNQTIELAKVISKTVSKIDDIQLGLDEKKNIEKRKFYVLIGDIIEIGFYYNLYLPDGKRNGIVEIIPYYKQYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XS1</accession>
    <name>Q97XS1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Penicillin acylase</fullName>
            <ecNumber evidence="4">3.5.1.11</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1638</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S45 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.11" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90324">
        <property type="entry name" value="D90324"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XS1"/>
    <dbReference type="MEROPS" id="S45.003"/>
    <dbReference type="PaxDb" id="273057-SSO1638"/>
    <dbReference type="EnsemblBacteria" id="AAK41851">
        <property type="protein sequence ID" value="AAK41851"/>
        <property type="gene ID" value="SSO1638"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1638"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1683"/>
    <dbReference type="eggNOG" id="arCOG04082">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011790_4_0_2"/>
    <dbReference type="InParanoid" id="Q97XS1"/>
    <dbReference type="PhylomeDB" id="Q97XS1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008953">
        <property type="term" value="F:penicillin amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017000">
        <property type="term" value="P:antibiotic biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.439.10">
        <property type="entry name" value="Penicillin Amidohydrolase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014395">
        <property type="entry name" value="Pen/GL7ACA/AHL_acylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043147">
        <property type="entry name" value="Penicillin_amidase_A-knob"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023343">
        <property type="entry name" value="Penicillin_amidase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043146">
        <property type="entry name" value="Penicillin_amidase_N_B-knob"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002692">
        <property type="entry name" value="S45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34218:SF4">
        <property type="entry name" value="ACYL-HOMOSERINE LACTONE ACYLASE QUIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34218">
        <property type="entry name" value="PEPTIDASE S45 PENICILLIN AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01804">
        <property type="entry name" value="Penicil_amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001227">
        <property type="entry name" value="Pen_acylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="829" mass="93362" checksum="13D713EC74979582" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XS8</accession>
    <name>Q97XS8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Coenzyme PQQ synthesis protein E (PqqE-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pqqE-2</name>
        <name evidence="8" type="ordered locus">SSO1631</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90323">
        <property type="entry name" value="E90323"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923531.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XS8"/>
    <dbReference type="STRING" id="273057.SSO1631"/>
    <dbReference type="PaxDb" id="273057-SSO1631"/>
    <dbReference type="DNASU" id="1454623"/>
    <dbReference type="EnsemblBacteria" id="AAK41844">
        <property type="protein sequence ID" value="AAK41844"/>
        <property type="gene ID" value="SSO1631"/>
    </dbReference>
    <dbReference type="GeneID" id="7806643"/>
    <dbReference type="KEGG" id="sso:SSO1631"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1673"/>
    <dbReference type="eggNOG" id="arCOG00938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_0_2"/>
    <dbReference type="InParanoid" id="Q97XS8"/>
    <dbReference type="PhylomeDB" id="Q97XS8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21123">
        <property type="entry name" value="SPASM_MftC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017200">
        <property type="entry name" value="PqqE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04053">
        <property type="entry name" value="TIGR04053 family radical SAM/SPASM domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF34">
        <property type="entry name" value="TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE COFACTOR MODIFYING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037420">
        <property type="entry name" value="PQQ_syn_pqqE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="4"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="350" mass="39509" checksum="C5A74FFF5ADF80DE" modified="2001-10-01" version="1">MPFENAPHLVFWEVTKACPLTCKHCRANAIDKPLPGELNTEESRKLLEDIARFGKVVIVFTGGDPLSRSDIFELMEYAKSLGLVVSIAPSPSHRLDDETMKIISNYARYMSISLDGATSQTHDWLRGLGSYKYALRGIELGLKYGIQVQVNTLVWKKSYSELPFVVKLLKEMGVKIWEVFFLIPVGRGTTELDIPRDKYKDVIDFLVEATRYDLIVRTVEAPFFRRAKLEYTPATTNDNELVSTLRELLGEPVKEADKSILPTRDGAGVIFIGYNGDVYPSGFLPLYLGNVKKESLVDIYRKSEVLKKIKDSRFEGKCGICKYNNICGGSRARAFAVYNNPFAEDPMCPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XW6</accession>
    <name>Q97XW6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Coenzyme F390 synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1594</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90318">
        <property type="entry name" value="D90318"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923493.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XW6"/>
    <dbReference type="STRING" id="273057.SSO1594"/>
    <dbReference type="PaxDb" id="273057-SSO1594"/>
    <dbReference type="EnsemblBacteria" id="AAK41803">
        <property type="protein sequence ID" value="AAK41803"/>
        <property type="gene ID" value="SSO1594"/>
    </dbReference>
    <dbReference type="GeneID" id="7806178"/>
    <dbReference type="KEGG" id="sso:SSO1594"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1628"/>
    <dbReference type="eggNOG" id="arCOG01870">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128032_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XW6"/>
    <dbReference type="PhylomeDB" id="Q97XW6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002808">
        <property type="entry name" value="AdoCbi_amidolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052209">
        <property type="entry name" value="CbiZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35336">
        <property type="entry name" value="ADENOSYLCOBINAMIDE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35336:SF5">
        <property type="entry name" value="ADENOSYLCOBINAMIDE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01955">
        <property type="entry name" value="CbiZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="180" mass="19627" checksum="B22A8C5D28D033EE" modified="2001-10-01" version="1">MEPKIVRFDLGKEYIILTSALYPEGIIRVNQICSIFVDKSYCSGNPWGDVINWCPSKSAIMFMTAAKNYTFKETDWGKFFISAGIGRSGEDAGCTINIGVFVDKGLNINGLVDLIRTVTEAKAGALRDLGYKFTGTVSDAIAVGSLPGNEYFIGPGTELGKKIAYDIRNTIVELLFKGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="149" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XZ3</accession>
    <name>Q97XZ3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Dihydrolipoamide dehydrogenase (PdhD-3)</fullName>
            <ecNumber evidence="12">1.8.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pdhD-3</name>
        <name evidence="12" type="ordered locus">SSO1565</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2009-12" db="PDB data bank">
            <title>Crystal structure of a dihydrolipoyl dehydrogenase from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Bonanno J.B."/>
                <person name="Rutter M."/>
                <person name="Bain K.T."/>
                <person name="Miller S."/>
                <person name="Sampathkumar P."/>
                <person name="Wasserman S."/>
                <person name="Sauder J.M."/>
                <person name="Burley S.K."/>
                <person name="Almo S.C."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 2-456 IN COMPLEX WITH ADP</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="12"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90315">
        <property type="entry name" value="C90315"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989987.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3L8K">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B=2-456"/>
    </dbReference>
    <dbReference type="PDBsum" id="3L8K"/>
    <dbReference type="AlphaFoldDB" id="Q97XZ3"/>
    <dbReference type="SMR" id="Q97XZ3"/>
    <dbReference type="FunCoup" id="Q97XZ3">
        <property type="interactions" value="249"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1565"/>
    <dbReference type="PaxDb" id="273057-SSO1565"/>
    <dbReference type="EnsemblBacteria" id="AAK41778">
        <property type="protein sequence ID" value="AAK41778"/>
        <property type="gene ID" value="SSO1565"/>
    </dbReference>
    <dbReference type="GeneID" id="1454557"/>
    <dbReference type="KEGG" id="sso:SSO1565"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1600"/>
    <dbReference type="eggNOG" id="arCOG01068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_2_2"/>
    <dbReference type="InParanoid" id="Q97XZ3"/>
    <dbReference type="PhylomeDB" id="Q97XZ3"/>
    <dbReference type="EvolutionaryTrace" id="Q97XZ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
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        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
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        <property type="entry name" value="PRK06292.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="14" id="KW-0002">3D-structure</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0274">FAD</keyword>
    <keyword evidence="3 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="11">
        <location>
            <begin position="3"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="10">
        <location>
            <begin position="343"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK41778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="14">
        <source>
            <dbReference type="PDB" id="3L8K"/>
        </source>
    </evidence>
    <sequence length="456" mass="50498" checksum="5380675B9F59C855" modified="2001-10-01" version="1">MKYDVVVIGAGGAGYHGAFRLAKAKYNVLMADPKGELGGNCLYSGCVPSKTVREVIQTAWRLTNIANVKIPLDFSTVQDRKDYVQELRFKQHKRNMSQYETLTFYKGYVKIKDPTHVIVKTDEGKEIEAETRYMIIASGAETAKLRLPGVEYCLTSDDIFGYKTSFRKLPQDMVIIGAGYIGLEIASIFRLMGVQTHIIEMLDRALITLEDQDIVNTLLSILKLNIKFNSPVTEVKKIKDDEYEVIYSTKDGSKKSIFTNSVVLAAGRRPVIPEGAREIGLSISKTGIVVDETMKTNIPNVFATGDANGLAPYYHAAVRMSIAAANNIMANGMPVDYVDVKSIPVTIYTIPSLSYVGILPSKARKMGIEIVEAEYNMEEDVSAQIYGQKEGVLKLIFERGSMRLIGAWMIGVHSQYLINELGLAVAYGLNAKQLASFAEQHPSTNEIISYTARKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y01</accession>
    <name>Q97Y01_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Second ORF in transposon ISC1395</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="C90314"/>
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    <dbReference type="AlphaFoldDB" id="Q97Y01"/>
    <dbReference type="STRING" id="273057.SSO1554"/>
    <dbReference type="PaxDb" id="273057-SSO1554"/>
    <dbReference type="EnsemblBacteria" id="AAK41770">
        <property type="protein sequence ID" value="AAK41770"/>
        <property type="gene ID" value="SSO1554"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1554"/>
    <dbReference type="eggNOG" id="arCOG02129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2056158_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y01"/>
    <dbReference type="PhylomeDB" id="Q97Y01"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="2">
        <location>
            <begin position="15"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="129" mass="14592" checksum="6E092B09554CAEB0" modified="2001-10-01" version="1">MRDRLKGVIKKGVKVFFTDESGIHHDPSRRLGLYNVPVDYPSRKLNVLASIPLFGGVPCFMFTYSNVNSEIFAEFLRLFRNSGPIVLILDNAKFHKNAYVFSVASQLGIILLFLPPYSPDLNPIEFGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y19</accession>
    <name>Q97Y19_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dihydrolipoamide S-acetyltransferase, carboxy-end (PdhC)</fullName>
            <ecNumber evidence="5">2.3.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pdhC</name>
        <name evidence="5" type="ordered locus">SSO1530</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.3.1.12" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99312">
        <property type="entry name" value="A99312"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y19"/>
    <dbReference type="FunCoup" id="Q97Y19">
        <property type="interactions" value="149"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1530"/>
    <dbReference type="PaxDb" id="273057-SSO1530"/>
    <dbReference type="EnsemblBacteria" id="AAK41752">
        <property type="protein sequence ID" value="AAK41752"/>
        <property type="gene ID" value="SSO1530"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1530"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1569"/>
    <dbReference type="eggNOG" id="arCOG01706">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_2_3_2"/>
    <dbReference type="InParanoid" id="Q97Y19"/>
    <dbReference type="PhylomeDB" id="Q97Y19"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004742">
        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050743">
        <property type="entry name" value="2-oxoacid_DH_E2_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43178">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43178:SF5">
        <property type="entry name" value="LIPOAMIDE ACYLTRANSFERASE COMPONENT OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="2-oxoacid dehydrogenase acyltransferase catalytic" evidence="4">
        <location>
            <begin position="2"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="177" mass="19666" checksum="0805927D2F0E4F62" modified="2001-10-01" version="1">MKITYTDILVKVVAKLLRDHPYLNATLEGDQIKIIEEVNIGIAVALDQGLIVPVIRNADTKPITEIAKESHELADKARENKLNPDEVSGGTFTISNLGMYDIDSFTPIINPPQTAILGVGRIRRAPVVVGDNISIGYIMWLSLTFDHRVMDGHTAAKFLKELTEILEDENKLRTFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y37</accession>
    <name>Q97Y37_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR type III-B/RAMP module-associated protein Cmr5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1513</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90309">
        <property type="entry name" value="G90309"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990107.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y37"/>
    <dbReference type="STRING" id="273057.SSO1513"/>
    <dbReference type="PaxDb" id="273057-SSO1513"/>
    <dbReference type="EnsemblBacteria" id="AAK41734">
        <property type="protein sequence ID" value="AAK41734"/>
        <property type="gene ID" value="SSO1513"/>
    </dbReference>
    <dbReference type="GeneID" id="1454513"/>
    <dbReference type="KEGG" id="sso:SSO1513"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1552"/>
    <dbReference type="eggNOG" id="arCOG02656">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1682783_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y37"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023101">
        <property type="entry name" value="AF1862-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158568">
        <property type="entry name" value="AF1862-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17457" checksum="9B6B6DEE6633496C" modified="2001-10-01" version="1">MESEYVKFATEIGKRIVSAQCKIQSGPSKPGLLRRSADFPSLMLGIGFSPAFTFYISKIEDYNRFKDIYNYLSGGKGNPASICKELDKREGSGYAGYVAIILLVLNNIGKRIRIGDDPKDIYYSLLDVASSVGSKEERLILPYLNEIKKVLEALPYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y58</accession>
    <name>Q97Y58_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">One of two inversely orientated ORFs in ISC1043</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1486</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90307">
        <property type="entry name" value="B90307"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923427.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y58"/>
    <dbReference type="PaxDb" id="273057-SSO1486"/>
    <dbReference type="EnsemblBacteria" id="AAK41713">
        <property type="protein sequence ID" value="AAK41713"/>
        <property type="gene ID" value="SSO1486"/>
    </dbReference>
    <dbReference type="GeneID" id="1454492"/>
    <dbReference type="KEGG" id="sso:SSO1486"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1524"/>
    <dbReference type="HOGENOM" id="CLU_2140319_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y58"/>
    <dbReference type="PhylomeDB" id="Q97Y58"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="112" mass="13313" checksum="F1F3BA3960261ECB" modified="2001-10-01" version="1">MNKKLTQKDFDNFKESIEDLIKLYKESRLSKIENNKESEEERLARLRRFGRELRQDLEEAKFVKIKKEQGRKSKLTPQEKAYILIIKEYLGLSNRETAELASLLNLTKELPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y83</accession>
    <name>Q97Y83_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRISPR-associated protein, Csa1 family</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90303">
        <property type="entry name" value="C90303"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923413.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y83"/>
    <dbReference type="STRING" id="273057.SSO1451"/>
    <dbReference type="PaxDb" id="273057-SSO1451"/>
    <dbReference type="EnsemblBacteria" id="AAK41682">
        <property type="protein sequence ID" value="AAK41682"/>
        <property type="gene ID" value="SSO1451"/>
    </dbReference>
    <dbReference type="GeneID" id="1454461"/>
    <dbReference type="KEGG" id="sso:SSO1451"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1481"/>
    <dbReference type="eggNOG" id="arCOG04195">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_905009_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y83"/>
    <dbReference type="PhylomeDB" id="Q97Y83"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09659">
        <property type="entry name" value="Cas4_I-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009260">
        <property type="entry name" value="CRISPR-ass_Csa1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01896">
        <property type="entry name" value="cas_AF1879"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06023">
        <property type="entry name" value="Csa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009226">
        <property type="entry name" value="UCP009226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="32244" checksum="1DD9313F33655651" modified="2001-10-01" version="1">MRSQIVRQLRRLHSYRASDPIEEELRGWNYYMPPIKPRRYLGLSISDVAYKYCETKRDVYLRKVLKIQGEQRTPLLLGQAIHEIVRNVSNEVVKLLSSGYKPWSVLETLFRNNKIVRSLCPPQFIKYCEEVYKAYALDLLSDFSDSTSFIPVISEFKVDGSPLGLSSRLSVDAIMQLSLVVEMKVGSVQEFHRLALAGYGMALESALELPIDFGALIYVNGINTSSPEFKVEVFYVSPDMRKEFLDARDEVIDMISQEKDPGMPVSCSPSCPFLNYCNNNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YB0</accession>
    <name>Q97YB0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1422</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90300">
        <property type="entry name" value="A90300"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YB0"/>
    <dbReference type="STRING" id="273057.SSO1422"/>
    <dbReference type="PaxDb" id="273057-SSO1422"/>
    <dbReference type="EnsemblBacteria" id="AAK41656">
        <property type="protein sequence ID" value="AAK41656"/>
        <property type="gene ID" value="SSO1422"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1422"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1448"/>
    <dbReference type="eggNOG" id="arCOG01440">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1297535_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YB0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010156">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01877">
        <property type="entry name" value="cas_cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="263" mass="29454" checksum="88B4570F947603BD" modified="2001-10-01" version="1">MQNTKCVISDITQHSYTFSGRFDMIVAEIFVKPESDAIIPFSSKVGKSLLLDPKNVSISPLKYKGKYLIKYGSVLTFLEVIGGNVYSFEVGGDERNVYSALINLGDKSLLFNTYWKVVDVEVHEVEVSSIPKNFGVDILTPALIVSPYVKEKRKVFTNKSEYVFFNNIIDATGFNRGDVKLSEVISRFAQLLWEEPSIIGYANVRYGDKLVIGMIGKLRYSVKGEDEVLIKVLEDAIVRGIGSSRRNGFGVVRIKGVEASWLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YB2</accession>
    <name>Q97YB2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acylaminoacyl-peptidase, putative (ApeH-1)</fullName>
            <ecNumber evidence="3">3.4.19.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">apeH-1</name>
        <name evidence="3" type="ordered locus">SSO1419</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.19.1" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90299">
        <property type="entry name" value="F90299"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988830.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YB2"/>
    <dbReference type="SMR" id="Q97YB2"/>
    <dbReference type="FunCoup" id="Q97YB2">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1419"/>
    <dbReference type="ESTHER" id="sulso-APEH1">
        <property type="family name" value="ACPH_Peptidase_S9"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1419"/>
    <dbReference type="EnsemblBacteria" id="AAK41653">
        <property type="protein sequence ID" value="AAK41653"/>
        <property type="gene ID" value="SSO1419"/>
    </dbReference>
    <dbReference type="GeneID" id="1454432"/>
    <dbReference type="KEGG" id="sso:SSO1419"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1444"/>
    <dbReference type="eggNOG" id="arCOG01646">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_482043_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YB2"/>
    <dbReference type="PhylomeDB" id="Q97YB2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000616">
        <property type="entry name" value="Putative peptidase S9 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF27">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE FAMILY MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69304">
        <property type="entry name" value="Tricorn protease N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="2">
        <location>
            <begin position="338"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="536" mass="61784" checksum="667EFCFD8D5FAEE1" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YF7</accession>
    <name>Q97YF7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Second ORF in transposon ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1368</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90293">
        <property type="entry name" value="C90293"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YF7"/>
    <dbReference type="PaxDb" id="273057-SSO1368"/>
    <dbReference type="DNASU" id="1454381"/>
    <dbReference type="EnsemblBacteria" id="AAK41602">
        <property type="protein sequence ID" value="AAK41602"/>
        <property type="gene ID" value="SSO1368"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1368"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1373"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_9_1_2"/>
    <dbReference type="InParanoid" id="Q97YF7"/>
    <dbReference type="PhylomeDB" id="Q97YF7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="24"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="149"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="240" mass="27930" checksum="FD3834BD9F941451" modified="2001-10-01" version="1">MVKHHWRVVVKLTRSERIYITFVVDHEFPKLPNTGKVVAIDVGVEKLLVTSDGEYFPNLRPYEKALWKVKHLHRELSRKKFLSHNWFKAKVKLARAYEHLKNLRTDLYMKLGKWFAEHYDVVVMEDIHAKQLVGKSLRSLRRRLSDVGFGELRDVLKYQLEKYGKKLILVNPAYTSKTCARCGYVKNDLSLSDRVFVCPNCGWIADRDYNASLNILRGSGSERPLVWSSALYQYSGKVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YG7</accession>
    <name>Q97YG7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Endoglucanase</fullName>
            <ecNumber evidence="3">3.2.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1354</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 12 (cellulase H) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90291">
        <property type="entry name" value="G90291"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923347.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YG7"/>
    <dbReference type="SMR" id="Q97YG7"/>
    <dbReference type="STRING" id="273057.SSO1354"/>
    <dbReference type="CAZy" id="GH12">
        <property type="family name" value="Glycoside Hydrolase Family 12"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1354"/>
    <dbReference type="EnsemblBacteria" id="AAK41590">
        <property type="protein sequence ID" value="AAK41590"/>
        <property type="gene ID" value="SSO1354"/>
    </dbReference>
    <dbReference type="GeneID" id="1454369"/>
    <dbReference type="KEGG" id="sso:SSO1354"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1360"/>
    <dbReference type="eggNOG" id="arCOG03858">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_768611_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YG7"/>
    <dbReference type="PhylomeDB" id="Q97YG7"/>
    <dbReference type="BRENDA" id="3.2.1.4">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="3.2.1.8">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013319">
        <property type="entry name" value="GH11/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002594">
        <property type="entry name" value="GH12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01670">
        <property type="entry name" value="Glyco_hydro_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="332" mass="37295" checksum="F896165AE4926B9E" modified="2001-10-01" version="1">MNKLYIVLPVIVIIAIGVMGGIIYLHQQSLSVKPVTTTEFSTTTSTSTTTNAITTTVTQTVTSITSYNQLIYVTSSASSPTPVYLNNSTIPSFYLEVNMWNAKNYNGNYTMVFNPLARTLSVSFNLTQVKPLEWTNGYPEIYVGRKPWDTAYAGNIFPMRIGNMTPFMVSFYINLTKLDPSINFDIASDAWIVRPQIAFSPGTAPGNGDIEIMVWLFSQNLQPAGEQVGKVVVPIYINHTLVNATFQVWEMKSVPWGGWEYIAFRPDGWKVTNGYVSYEPNLFIKALSNFTSYNITNYYLTDWEFGTEWGTMTSNGTAYFSWTVSNFSETLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YH9</accession>
    <name>Q97YH9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3782 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1344</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90290">
        <property type="entry name" value="E90290"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YH9"/>
    <dbReference type="PaxDb" id="273057-SSO1344"/>
    <dbReference type="EnsemblBacteria" id="AAK41580">
        <property type="protein sequence ID" value="AAK41580"/>
        <property type="gene ID" value="SSO1344"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1344"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1349"/>
    <dbReference type="eggNOG" id="arCOG01422">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064028_1_0_2"/>
    <dbReference type="InParanoid" id="Q97YH9"/>
    <dbReference type="PhylomeDB" id="Q97YH9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024271">
        <property type="entry name" value="DUF3782"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012431">
        <property type="entry name" value="PDDEXK_10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34314">
        <property type="entry name" value="CRENARCHAEAL PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34314:SF7">
        <property type="entry name" value="DUF3782 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12644">
        <property type="entry name" value="DUF3782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07788">
        <property type="entry name" value="PDDEXK_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="25052" checksum="B3BEC595CC5C1EC5" modified="2001-10-01" version="1">MKMSLAEEIKKVLKENPSIIVDALAQRPEILYDVLVKLMPWQVIIRDIDDLKNHTQKIEKELGEVKESLKNFVTKEDAKNFATKDDIKRLETIITGLGARWGLLSEDAFRQGVYEIMKSEGIVAKKELVYDKTGEVYGEPSDVEYDLMIEDGNLVMIEITSAIKRGDLPVIRKKKEFYERNKNVKISKVIVITPFLHDKYPDRLKAMAKDMGIEIINP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YI0</accession>
    <name>Q97YI0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">L-carnitine dehydratase, putative (CaiB)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">caiB</name>
        <name evidence="1" type="ordered locus">SSO1343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90290">
        <property type="entry name" value="D90290"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923336.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YI0"/>
    <dbReference type="STRING" id="273057.SSO1343"/>
    <dbReference type="PaxDb" id="273057-SSO1343"/>
    <dbReference type="DNASU" id="1454358"/>
    <dbReference type="EnsemblBacteria" id="AAK41579">
        <property type="protein sequence ID" value="AAK41579"/>
        <property type="gene ID" value="SSO1343"/>
    </dbReference>
    <dbReference type="GeneID" id="1454358"/>
    <dbReference type="KEGG" id="sso:SSO1343"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1347"/>
    <dbReference type="eggNOG" id="arCOG02304">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_2_1_2"/>
    <dbReference type="InParanoid" id="Q97YI0"/>
    <dbReference type="PhylomeDB" id="Q97YI0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="entry name" value="Crotonobetainyl-coa:carnitine coa-transferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="entry name" value="formyl-coa transferase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050509">
        <property type="entry name" value="CoA-transferase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48228:SF5">
        <property type="entry name" value="ALPHA-METHYLACYL-COA RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48228">
        <property type="entry name" value="SUCCINYL-COA--D-CITRAMALATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="CoA-transferase family III (CaiB/BaiF)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="366" mass="41915" checksum="9BA31F7FCAC9CC07" modified="2001-10-01" version="1">MYRVIELGHVISAPFAGEILRHLGFEVIKIEPLLGDPSRKDDVLVDSMFVFNNRGKRSISVDLRKEKGKEVFLRLVRNSHVLIENLSPHVMDRLGLSDDVIFSTNPSLVYCSIKGYPKGKYENLPAFGTIIEAESGIMDANGKARLPASITDMNASTYCVITILWALLMKKPGHYRVSIIQANTVWLGYYLIAYQKYGKLFEAGKDELPFWAPYELFKSSEEKEFYLAVNDNEKFTKLCKALGLEDLLADERFKTNADRVRNRKILHEKLQQKFSSMKLEEIMNILRYNDIPVGKLNNLKDLISNELVEWDTFRNVQVPKLPLPGSLNSQHVPNVGEDTLYILKELGYSENEIESMIKENVINYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YJ3</accession>
    <name>Q97YJ3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90288">
        <property type="entry name" value="G90288"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923323.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YJ3"/>
    <dbReference type="PaxDb" id="273057-SSO1330"/>
    <dbReference type="EnsemblBacteria" id="AAK41566">
        <property type="protein sequence ID" value="AAK41566"/>
        <property type="gene ID" value="SSO1330"/>
    </dbReference>
    <dbReference type="GeneID" id="71027429"/>
    <dbReference type="KEGG" id="sso:SSO1330"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1330"/>
    <dbReference type="eggNOG" id="arCOG03169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631732_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YJ3"/>
    <dbReference type="PhylomeDB" id="Q97YJ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301:SF8">
        <property type="entry name" value="ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301">
        <property type="entry name" value="DNA-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="14"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="162" mass="18690" checksum="6F3AA9931560E95C" modified="2001-10-01" version="1">MLFDERPKENREDLFDREKEIKEIMSNINRPLILITGVRRIGKTSVLKVALNEIKIPHVIIDCRNLRPNYSRGDLYSLFSNAFSSKLSTFLDVLSKIRGVSILGNSVELKWKGREYVSLSDLFDHLNEKRIVIGIDEAQKLRGPLSNEIKEAIAHAYDYDKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2023-05-03" modified="2026-01-28" version="148" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YM2</accession>
    <name>ARADH_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">D-arabinose 1-dehydrogenase (NADP(+))</fullName>
            <shortName evidence="3">AraDH</shortName>
            <shortName evidence="3">D-Ara dehydrogenase</shortName>
            <ecNumber evidence="1">1.1.1.427</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">araDH</name>
        <name evidence="5" type="synonym">adh-4</name>
        <name evidence="5" type="ordered locus">SSO1300</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="J. Biol. Chem." volume="281" first="27378" last="27388">
            <title>Identification of the missing links in prokaryotic pentose oxidation pathways: evidence for enzyme recruitment.</title>
            <authorList>
                <person name="Brouns S.J."/>
                <person name="Walther J."/>
                <person name="Snijders A.P."/>
                <person name="van de Werken H.J."/>
                <person name="Willemen H.L."/>
                <person name="Worm P."/>
                <person name="de Vos M.G."/>
                <person name="Andersson A."/>
                <person name="Lundgren M."/>
                <person name="Mazon H.F."/>
                <person name="van den Heuvel R.H."/>
                <person name="Nilsson P."/>
                <person name="Salmon L."/>
                <person name="de Vos W.M."/>
                <person name="Wright P.C."/>
                <person name="Bernander R."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="16849334"/>
            <dbReference type="DOI" id="10.1074/jbc.M605549200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>INDUCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="journal article" date="2007" name="J. Mol. Biol." volume="371" first="1249" last="1260">
            <title>Crystal structure and biochemical properties of the D-arabinose dehydrogenase from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Brouns S.J."/>
                <person name="Turnbull A.P."/>
                <person name="Willemen H.L."/>
                <person name="Akerboom J."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="17610898"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2007.05.097"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 6-349 IN COMPLEX WITH ZINC</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>DOMAIN</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Participates in a pentose oxidation pathway that converts D-arabinose to 2-oxoglutarate (PubMed:16849334). Catalyzes the NADP-dependent conversion of D-arabinose to D-arabinono-1,4-lactone (PubMed:16849334, PubMed:17610898). In vitro, can also use L-fucose, L-galactose and D-ribose (PubMed:16849334, PubMed:17610898). Shows highest activity with L-fucose, in combinaison with NAD, and lower activity toward L-galactose and D-ribose (PubMed:16849334, PubMed:17610898). When acting on its physiological substrate, D-arabinose, shows a clear preference for NADP over NAD (PubMed:16849334).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>D-arabinose + NADP(+) = D-arabinono-1,4-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21892"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16292"/>
            <dbReference type="ChEBI" id="CHEBI:46994"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.427"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:21893"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1 2">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">1.4 mM for D-arabinose</KM>
            <KM evidence="2">0.22 mM for L-fucose</KM>
            <KM evidence="1">0.04 mM for NADP(+)</KM>
            <KM evidence="2">0.038 mM for NADP(+)</KM>
            <KM evidence="1 2">1.25 mM for NAD(+)</KM>
            <Vmax evidence="2">23.8 umol/min/mg enzyme with D-arabinose as substrate</Vmax>
            <Vmax evidence="2">23.7 umol/min/mg enzyme with L-fucose as substrate</Vmax>
            <Vmax evidence="2">32.1 umol/min/mg enzyme with NADP(+) as substrate</Vmax>
            <Vmax evidence="2">29.1 umol/min/mg enzyme with NAD(+) as substrate</Vmax>
            <text evidence="1">kcat is 23.8 sec(-1) with D-arabinose as substrate. kcat is 26.8 sec(-1) with L-fucose as substrate. kcat is 17.7 sec(-1) with L-galactose or D-ribose as substrate.</text>
        </kinetics>
        <phDependence>
            <text evidence="1 2">Optimum pH is 8.2 (PubMed:16849334, PubMed:17610898). Is more than 50% active in a relatively narrow pH range from 7.3 to 9.3 (PubMed:17610898).</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="1 2">Optimum temperature is 91 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Homotetramer (PubMed:16849334, PubMed:17610898). Dimer of dimers (PubMed:17610898).</text>
    </comment>
    <comment type="induction">
        <text evidence="1">Expression is highly induced during growth on D-arabinose. The promoter contains the conserved cis-regulatory element 5'-AACATGTT-3' ARA-box found in arabinose-induced genes.</text>
    </comment>
    <comment type="domain">
        <text evidence="2">Contains two domains: a catalytic domain (residues 1-154 and 292-344) and a nucleotide binding domain (residues 155-291) (PubMed:17610898). The active site resides in a deep cleft between the domains (PubMed:17610898).</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.427" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90285">
        <property type="entry name" value="B90285"/>
    </dbReference>
    <dbReference type="PDB" id="2H6E">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A=6-349"/>
    </dbReference>
    <dbReference type="PDBsum" id="2H6E"/>
    <dbReference type="AlphaFoldDB" id="Q97YM2"/>
    <dbReference type="SMR" id="Q97YM2"/>
    <dbReference type="FunCoup" id="Q97YM2">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1300"/>
    <dbReference type="PaxDb" id="273057-SSO1300"/>
    <dbReference type="EnsemblBacteria" id="AAK41537">
        <property type="protein sequence ID" value="AAK41537"/>
        <property type="gene ID" value="SSO1300"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1300"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1301"/>
    <dbReference type="eggNOG" id="arCOG01457">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_2_2"/>
    <dbReference type="InParanoid" id="Q97YM2"/>
    <dbReference type="PhylomeDB" id="Q97YM2"/>
    <dbReference type="BioCyc" id="MetaCyc:MONOMER-13204"/>
    <dbReference type="BRENDA" id="1.1.1.122">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="1.1.1.B35">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97YM2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030554">
        <property type="term" value="F:adenyl nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043168">
        <property type="term" value="F:anion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106271">
        <property type="term" value="F:D-arabinose 1-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008743">
        <property type="term" value="F:L-threonine 3-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019568">
        <property type="term" value="P:arabinose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006566">
        <property type="term" value="P:threonine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05284">
        <property type="entry name" value="arabinose_DH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF4">
        <property type="entry name" value="D-ARABINOSE 1-DEHYDROGENASE (NADP(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0054">Arabinose catabolism</keyword>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000457928" description="D-arabinose 1-dehydrogenase (NADP(+))">
        <location>
            <begin position="1"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2 8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 8">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
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        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="2 8">
        <location>
            <position position="102"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>2</label>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="2 8">
        <location>
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        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="2 8">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 8">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="7"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
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        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
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        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
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        </location>
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    <feature type="strand" evidence="9">
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    </feature>
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        <location>
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        </location>
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        <location>
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        <location>
            <begin position="254"/>
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        </location>
    </feature>
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        <location>
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        </location>
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        </location>
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        </location>
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        <location>
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        </location>
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        <source>
            <dbReference type="PubMed" id="16849334"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PubMed" id="17610898"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PubMed" id="16849334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="2H6E"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="2H6E"/>
        </source>
    </evidence>
    <sequence length="349" mass="37879" checksum="01028CE2C5027320" modified="2001-10-01" version="1">MENVNMVKSKAALLKKFSEPLSIEDVNIPEPQGEEVLIRIGGAGVCRTDLRVWKGVEAKQGFRLPIILGHENAGTIVEVGELAKVKKGDNVVVYATWGDLTCRYCREGKFNICKNQIIPGQTTNGGFSEYMLVKSSRWLVKLNSLSPVEAAPLADAGTTSMGAIRQALPFISKFAEPVVIVNGIGGLAVYTIQILKALMKNITIVGISRSKKHRDFALELGADYVSEMKDAESLINKLTDGLGASIAIDLVGTEETTYNLGKLLAQEGAIILVGMEGKRVSLEAFDTAVWNKKLLGSNYGSLNDLEDVVRLSESGKIKPYIIKVPLDDINKAFTNLDEGRVDGRQVITP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YQ3</accession>
    <name>Q97YQ3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alanyl-tRNA synthetase truncated homolog (AlaS-like2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">alaS-like2</name>
        <name evidence="1" type="ordered locus">SSO1271</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="E90281"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="PaxDb" id="273057-SSO1271"/>
    <dbReference type="EnsemblBacteria" id="AAK41508">
        <property type="protein sequence ID" value="AAK41508"/>
        <property type="gene ID" value="SSO1271"/>
    </dbReference>
    <dbReference type="GeneID" id="7808497"/>
    <dbReference type="KEGG" id="sso:SSO1271"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1267"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1656933_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140101">
        <property type="term" value="F:catalytic activity, acting on a tRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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        <property type="term" value="P:tRNA metabolic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
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        <property type="entry name" value="ThrRS/AlaRS common domain"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="150" mass="17522" checksum="5A02BC52838A561F" modified="2001-10-01" version="1">MDQVEIRTHTALHVVKGAVRKVLGAKWTASTYVNSNHGRLTVKFERKPSDQEMDKVFELANEKVRKNLPIIIEVLPREEAERKYGDEIYDLFLISAEVRELSIVVIPDWSINACNKQHTKFTSEIGEIIKDYWRYRNSKQLLEISFDIKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YT7</accession>
    <name>Q97YT7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alcohol dehydrogenase (Zn containing) (Adh-3)</fullName>
            <ecNumber evidence="8">1.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">adh-3</name>
        <name evidence="8" type="ordered locus">SSO1220</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90276">
        <property type="entry name" value="D90276"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YT7"/>
    <dbReference type="FunCoup" id="Q97YT7">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1220"/>
    <dbReference type="PaxDb" id="273057-SSO1220"/>
    <dbReference type="EnsemblBacteria" id="AAK41467">
        <property type="protein sequence ID" value="AAK41467"/>
        <property type="gene ID" value="SSO1220"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1220"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1221"/>
    <dbReference type="eggNOG" id="arCOG01455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_2_2"/>
    <dbReference type="InParanoid" id="Q97YT7"/>
    <dbReference type="PhylomeDB" id="Q97YT7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030554">
        <property type="term" value="F:adenyl nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043168">
        <property type="term" value="F:anion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008743">
        <property type="term" value="F:L-threonine 3-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006566">
        <property type="term" value="P:threonine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08260">
        <property type="entry name" value="Zn_ADH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF4">
        <property type="entry name" value="D-ARABINOSE 1-DEHYDROGENASE (NADP(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="16"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="362" mass="39683" checksum="B2308E89570DBFFA" modified="2001-10-01" version="1">MMKAARLVEFQKPLKIEDLDVPKVGKGDVLLKVISCGICRSDWHLWRGDPALVAYMQWSGGKLPITQGHEVYGEIVEVGESVTRLNKGDKVVMPASSTGDNRTCKYCMEGDSNVCEHLVIAGYGINGCFAEYMLIPERSVIDLVKVPDGIKSEWAALSSCGFGTSWNAFTMKTNLKPSDIVVIIGAGGMGLSGIAIANALGAKVIAIDVNERSLEKAKMLGSVETYRHSGKSEELNKIVEDIMKKYGSVDVIYDTTGIPEAVIPFLPLLKVHGTLLLAGLMMKGKEIFPLPADLVVAREIRIQGVLMLPAQKFDTIFKLMKEGKINLDPVIYKTISIYDVNDAYKEMSEYKNAGRIIINKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YV2</accession>
    <name>Q97YV2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Third ORF in transposon ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1203</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90274">
        <property type="entry name" value="D90274"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YV2"/>
    <dbReference type="STRING" id="273057.SSO1203"/>
    <dbReference type="PaxDb" id="273057-SSO1203"/>
    <dbReference type="EnsemblBacteria" id="AAK41451">
        <property type="protein sequence ID" value="AAK41451"/>
        <property type="gene ID" value="SSO1203"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1203"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1202"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1640043_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YV2"/>
    <dbReference type="PhylomeDB" id="Q97YV2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="173" mass="19882" checksum="E97BE67AFBD20D1E" modified="2001-10-01" version="1">MNVKARVFDSWYVNSRTLQWNTVGELKSSARVVEGGRSVPVSEFPQGEYLVEYLGTPIKLLVIDDYKGYGRRYFFSTDLNDTAEDIITTWENRWDIEVLIRELKALGLEGGSFLTWVRNSGFVALKALSLLVVQYFKYSTGLMLGAKRLARLIKSIYREAGGIKKLFKRRRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YY6</accession>
    <name>Q97YY6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transposase ISC1250</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1164</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90269">
        <property type="entry name" value="G90269"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991578.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YY6"/>
    <dbReference type="PaxDb" id="273057-SSO1164"/>
    <dbReference type="EnsemblBacteria" id="AAK41414">
        <property type="protein sequence ID" value="AAK41414"/>
        <property type="gene ID" value="SSO1164"/>
    </dbReference>
    <dbReference type="GeneID" id="1454193"/>
    <dbReference type="KEGG" id="sso:SSO1164"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1158"/>
    <dbReference type="eggNOG" id="arCOG04918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074510_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YY6"/>
    <dbReference type="PhylomeDB" id="Q97YY6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217:SF7">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00872">
        <property type="entry name" value="Transposase_mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0815">Transposition</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="360" mass="41378" checksum="B85A03139C8E4C3B" modified="2001-10-01" version="1">MELLTVNEVALYGVKLRELVEGPGQSSTLTLVPKILPDDALLNKSVPEIERMALEEAERVSPNYRRGKEVKRKGYARYRFLKGFRIVMVGREMRYELEWISVRLPVLYCEGRVRTQVEETLLREERKVFMALMAVYSVKGGKLNAKIWMPEIEIGGDFRYVIVDGKYVKLKRRKAVLLVAIGVTQEGRRAVLEVIVSEAEDTMAYWSLLVRVWKRSSFVLVVADGIQALDKAISLAELHVGRQGCLVHLKRRATKEEREALDAITSSAELGEIKPETNPTLLSYLVASKELWKWLKTNNLVESFNSLLERRRFGSFHSPWRILQIARAIALYYNLLTYFLTIVIILQSSSFLPIPQKYIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z18</accession>
    <name>Q97Z18_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2010-03" db="PDB data bank">
            <title>Crystal structure of DsrE/DsrF-like family protein (NP_342589.1) from SULFOLOBUS SOLFATARICUS at 1.49 A resolution.</title>
            <authorList>
                <consortium name="Joint Center for Structural Genomics (JCSG)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.49 ANGSTROMS)</scope>
        <scope>CYSTEINE SULFENIC ACID (-SOH) AT CYS-93</scope>
        <scope>METHYLATION AT LYS-14; LYS-15; LYS-16; LYS-55; LYS-58; LYS-62; LYS-63; LYS-70; LYS-71; LYS-84; LYS-89; LYS-98 AND LYS-104</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90265">
        <property type="entry name" value="D90265"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989755.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3MC3">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.49 A"/>
        <property type="chains" value="A=1-133"/>
    </dbReference>
    <dbReference type="PDBsum" id="3MC3"/>
    <dbReference type="AlphaFoldDB" id="Q97Z18"/>
    <dbReference type="SMR" id="Q97Z18"/>
    <dbReference type="STRING" id="273057.SSO1125"/>
    <dbReference type="PaxDb" id="273057-SSO1125"/>
    <dbReference type="EnsemblBacteria" id="AAK41379">
        <property type="protein sequence ID" value="AAK41379"/>
        <property type="gene ID" value="SSO1125"/>
    </dbReference>
    <dbReference type="GeneID" id="1454158"/>
    <dbReference type="KEGG" id="sso:SSO1125"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1119"/>
    <dbReference type="eggNOG" id="arCOG02066">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139144_1_1_2"/>
    <dbReference type="InParanoid" id="Q97Z18"/>
    <dbReference type="PhylomeDB" id="Q97Z18"/>
    <dbReference type="EvolutionaryTrace" id="Q97Z18"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655">
        <property type="entry name" value="CONSERVED WITHIN P. AEROPHILUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655:SF2">
        <property type="entry name" value="PEROXIREDOXIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DsrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="3" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6,N6-dimethyllysine" evidence="3">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6,N6-dimethyllysine" evidence="3">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6,N6-dimethyllysine" evidence="3">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6,N6-dimethyllysine" evidence="3">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6,N6-dimethyllysine" evidence="3">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6,N6-dimethyllysine" evidence="3">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6,N6-dimethyllysine" evidence="3">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6,N6-dimethyllysine" evidence="3">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6,N6-dimethyllysine" evidence="3">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6,N6-dimethyllysine" evidence="3">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6,N6-dimethyllysine" evidence="3">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="modified residue" description="Cysteine sulfenic acid (-SOH)" evidence="3">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6,N6-dimethyllysine" evidence="3">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6,N6-dimethyllysine" evidence="3">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="3MC3"/>
        </source>
    </evidence>
    <sequence length="133" mass="15201" checksum="CF236493763259CC" modified="2001-10-01" version="1">MAQAQTQGQEEEQKKKILIVVTHGPEDLDRTYAPLFMASISASMEYETSVFFMIKGPKLLDKKWQEEERKKGGNPFIHFFDMAKENGVKMYVCVQSLKDMCHMKEDDVVEGIELVGGSTLIDLTLEADRTLFF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z34</accession>
    <name>GCSH3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable glycine cleavage system H protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gcvH3</name>
        <name type="ordered locus">SSO1105</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="1">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90263">
        <property type="entry name" value="C90263"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989738.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z34"/>
    <dbReference type="SMR" id="Q97Z34"/>
    <dbReference type="FunCoup" id="Q97Z34">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1105"/>
    <dbReference type="PaxDb" id="273057-SSO1105"/>
    <dbReference type="EnsemblBacteria" id="AAK41362">
        <property type="protein sequence ID" value="AAK41362"/>
        <property type="gene ID" value="SSO1105"/>
    </dbReference>
    <dbReference type="GeneID" id="1454141"/>
    <dbReference type="KEGG" id="sso:SSO1105"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1102"/>
    <dbReference type="eggNOG" id="arCOG01303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097408_2_2_2"/>
    <dbReference type="InParanoid" id="Q97Z34"/>
    <dbReference type="PhylomeDB" id="Q97Z34"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715:SF3">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0450">Lipoyl</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000166284" description="Probable glycine cleavage system H protein 3">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="2">
        <location>
            <begin position="29"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="1">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01066"/>
        </source>
    </evidence>
    <sequence length="140" mass="16336" checksum="B6E9BC0E48ED1DCB" modified="2001-10-01" version="1">MKILGFTFPDDLLYEPEKHVWVRIEDNSVVSIGVTDLGQYMAGKIFQVTAKQKGEKVNGRSVLFSIESAKWIGKFRLPIEGEVFDVNEEVVKNPSIINERPYDSWIVKIRVEDMDIIKRTFKPIQEVYKQFEEEAKRVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z76</accession>
    <name>Q97Z76_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90257">
        <property type="entry name" value="F90257"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z76"/>
    <dbReference type="PaxDb" id="273057-SSO1053"/>
    <dbReference type="EnsemblBacteria" id="AAK41317">
        <property type="protein sequence ID" value="AAK41317"/>
        <property type="gene ID" value="SSO1053"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1053"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1049"/>
    <dbReference type="eggNOG" id="arCOG07724">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_429396_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z76"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="637" mass="74022" checksum="17EF843BB5AB13BF" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z87</accession>
    <name>Q97Z87_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">tRNA (guanine(10)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="12">2.1.1.213</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">tRNA:G10 dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">SSO1044</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the adenosylmethionine-dependent methylation of the exocyclic amino group (N(2)) of guanosine at position 10 of various tRNAs. Acts via a two-step process that leads to the formation of either N(2)-monomethyl (m(2)G) or N(2)-dimethylguanosine (m(2)(2)G).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>guanosine(10) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(10) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43124"/>
            <dbReference type="Rhea" id="RHEA-COMP:10355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10358"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.213"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the methyltransferase superfamily. Trm-G10 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.213" evidence="12"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90256">
        <property type="entry name" value="C90256"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923175.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z87"/>
    <dbReference type="STRING" id="273057.SSO1044"/>
    <dbReference type="PaxDb" id="273057-SSO1044"/>
    <dbReference type="DNASU" id="1454085"/>
    <dbReference type="EnsemblBacteria" id="AAK41306">
        <property type="protein sequence ID" value="AAK41306"/>
        <property type="gene ID" value="SSO1044"/>
    </dbReference>
    <dbReference type="GeneID" id="1454085"/>
    <dbReference type="KEGG" id="sso:SSO1044"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1038"/>
    <dbReference type="eggNOG" id="arCOG00047">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057819_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z87"/>
    <dbReference type="PhylomeDB" id="Q97Z87"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160101">
        <property type="term" value="F:tRNA (guanine(10)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000251">
        <property type="entry name" value="Putative RNA methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF3">
        <property type="entry name" value="TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00507">
        <property type="entry name" value="N12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase" evidence="14">
        <location>
            <begin position="143"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAK41306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="314" mass="35529" checksum="397D8BB57242950B" modified="2001-10-01" version="1">MKTTSYAVLKGNNYFLSLAELRALLNDDNAEINYFTGVAIFEGNKGDIANRSARIKRSGELVYISDDPKKINEMLRGGCFWIKEDVIMGSQKDKLNAINYEIKRGVKVSKECEKIDIILTDGVIILGKIKGQIDSKSLHEHEKKPFSQSGTMNPETSRLLVNLSRPKKEVLDPFVGTGSILIEARWLNYECIGSDLDKAMLQKTKINLNYFNYYCSLLFSSATNLPFRQITSIATDPPYGRSTKNKGAELTQLYEEFFSSASETLTKGGFLVFATDSRFNFIDKLKENSFIIKGLHYLYSHKSLTRAIYVVQKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-07-22" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZE3</accession>
    <name>CREN7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chromatin protein Cren7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">creN7</name>
        <name type="ordered locus">SSO6901</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2022" name="Biomolecules" volume="12" first="0" last="0">
            <title>Interplay between Alba and Cren7 Regulates Chromatin Compaction in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Cajili M.K.M."/>
                <person name="Prieto E.I."/>
            </authorList>
            <dbReference type="PubMed" id="35454068"/>
            <dbReference type="DOI" id="10.3390/biom12040481"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2022" name="Int. J. Biol. Macromol." volume="214" first="381" last="390">
            <title>Essentiality of core hydrophobicity to the structure and function of archaeal chromatin protein Cren7.</title>
            <authorList>
                <person name="Tian L."/>
                <person name="Ding N."/>
                <person name="Liu X."/>
                <person name="Chen Y."/>
                <person name="Zhang Z."/>
            </authorList>
            <dbReference type="PubMed" id="35728637"/>
            <dbReference type="DOI" id="10.1016/j.ijbiomac.2022.06.114"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>MUTAGENESIS OF PHE-41</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2022" name="Phys. Chem. Chem. Phys." volume="24" first="19401" last="19413">
            <title>Structural and thermodynamic insights into the Cren7 mediated DNA organization in Crenarchaeota.</title>
            <authorList>
                <person name="K G."/>
                <person name="Thomas A.R."/>
                <person name="Vyjayanthi T.S."/>
                <person name="Mandal S.S."/>
            </authorList>
            <dbReference type="PubMed" id="35920727"/>
            <dbReference type="DOI" id="10.1039/d2cp02190k"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2023" name="Biophys. J." volume="122" first="1701" last="1719">
            <title>Molecular contacts in the Cren7-DNA complex: A quantitative investigation for electrostatic interaction.</title>
            <authorList>
                <person name="K G."/>
                <person name="Verma A."/>
                <person name="Mondal P."/>
                <person name="Mandal S.S."/>
            </authorList>
            <dbReference type="PubMed" id="37016575"/>
            <dbReference type="DOI" id="10.1016/j.bpj.2023.03.041"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>MUTAGENESIS OF LYS-24; LYS-31; LYS-42 AND LYS-48</scope>
    </reference>
    <reference evidence="10" key="6">
        <citation type="journal article" date="2008" name="Nucleic Acids Res." volume="36" first="1129" last="1137">
            <title>Biochemical and structural characterization of Cren7, a novel chromatin protein conserved among Crenarchaea.</title>
            <authorList>
                <person name="Guo L."/>
                <person name="Feng Y."/>
                <person name="Zhang Z."/>
                <person name="Yao H."/>
                <person name="Luo Y."/>
                <person name="Wang J."/>
                <person name="Huang L."/>
            </authorList>
            <dbReference type="PubMed" id="18096617"/>
            <dbReference type="DOI" id="10.1093/nar/gkm1128"/>
        </citation>
        <scope>STRUCTURE BY NMR</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>DEVELOPMENTAL STAGE</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="11" key="7">
        <citation type="journal article" date="2010" name="Protein Sci." volume="19" first="1253" last="1257">
            <title>Crystal structure of the crenarchaeal conserved chromatin protein Cren7 and double-stranded DNA complex.</title>
            <authorList>
                <person name="Feng Y."/>
                <person name="Yao H."/>
                <person name="Wang J."/>
            </authorList>
            <dbReference type="PubMed" id="20512977"/>
            <dbReference type="DOI" id="10.1002/pro.385"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 6-60 IN COMPLEX WITH DNA</scope>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="3 4 5 6 7 8">A highly abundant chromatin protein, it binds double-stranded DNA without sequence specificity; there is approximately 1 Cren7 molecule for 10-12 bp of DNA (PubMed:18096617, PubMed:20512977, PubMed:35728637, PubMed:35920727, PubMed:37016575). Constrains negative DNA supercoils (PubMed:18096617, PubMed:35728637). Induces DNA compaction and mediates intermolecular DNA bridging (PubMed:35728637). Binding does not require protein methylation (PubMed:18096617). Binds single-stranded DNA weakly (PubMed:18096617). Increases DNA stability against thermal denaturation (PubMed:18096617, PubMed:35920727, PubMed:37016575). Promotes formation of loose nucleoprotein structures with long DNA in the presence of albA proteins (PubMed:35454068).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="developmental stage">
        <text evidence="3">Cellular levels are constant during the growth cycle.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Methylated at multiple sites, to varying extents.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Cren7 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90249">
        <property type="entry name" value="B90249"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992425.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2JTM">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-60"/>
    </dbReference>
    <dbReference type="PDB" id="3KXT">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
        <property type="chains" value="A=6-60"/>
    </dbReference>
    <dbReference type="PDB" id="3LWH">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A=1-60"/>
    </dbReference>
    <dbReference type="PDB" id="3LWI">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B=1-60"/>
    </dbReference>
    <dbReference type="PDB" id="4R55">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A=1-60"/>
    </dbReference>
    <dbReference type="PDB" id="4R56">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B=1-60"/>
    </dbReference>
    <dbReference type="PDB" id="5K07">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-60"/>
    </dbReference>
    <dbReference type="PDB" id="5K17">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B=1-60"/>
    </dbReference>
    <dbReference type="PDB" id="5WVW">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B=1-60"/>
    </dbReference>
    <dbReference type="PDB" id="5WVY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B=1-60"/>
    </dbReference>
    <dbReference type="PDB" id="5WVZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B=1-60"/>
    </dbReference>
    <dbReference type="PDB" id="5WWC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A/B=1-60"/>
    </dbReference>
    <dbReference type="PDB" id="6A2H">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A=1-60"/>
    </dbReference>
    <dbReference type="PDB" id="6A2I">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=1-60"/>
    </dbReference>
    <dbReference type="PDBsum" id="2JTM"/>
    <dbReference type="PDBsum" id="3KXT"/>
    <dbReference type="PDBsum" id="3LWH"/>
    <dbReference type="PDBsum" id="3LWI"/>
    <dbReference type="PDBsum" id="4R55"/>
    <dbReference type="PDBsum" id="4R56"/>
    <dbReference type="PDBsum" id="5K07"/>
    <dbReference type="PDBsum" id="5K17"/>
    <dbReference type="PDBsum" id="5WVW"/>
    <dbReference type="PDBsum" id="5WVY"/>
    <dbReference type="PDBsum" id="5WVZ"/>
    <dbReference type="PDBsum" id="5WWC"/>
    <dbReference type="PDBsum" id="6A2H"/>
    <dbReference type="PDBsum" id="6A2I"/>
    <dbReference type="AlphaFoldDB" id="Q97ZE3"/>
    <dbReference type="SMR" id="Q97ZE3"/>
    <dbReference type="STRING" id="273057.SSO6901"/>
    <dbReference type="PaxDb" id="273057-SSO6901"/>
    <dbReference type="EnsemblBacteria" id="AAK41249">
        <property type="protein sequence ID" value="AAK41249"/>
        <property type="gene ID" value="SSO6901"/>
    </dbReference>
    <dbReference type="GeneID" id="1455216"/>
    <dbReference type="GeneID" id="44129907"/>
    <dbReference type="KEGG" id="sso:SSO6901"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.974"/>
    <dbReference type="eggNOG" id="arCOG04114">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2911298_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZE3"/>
    <dbReference type="EvolutionaryTrace" id="Q97ZE3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.610">
        <property type="entry name" value="Chromatin protein Cren7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01387">
        <property type="entry name" value="Chromatin_Cren7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038647">
        <property type="entry name" value="Cren7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020906">
        <property type="entry name" value="dsDNA-bd_Cren7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11520">
        <property type="entry name" value="Cren7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0158">Chromosome</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000345178" description="Chromatin protein Cren7">
        <location>
            <begin position="2"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine" evidence="1">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Slightly reduces the melting temperature of the protein. Slightly reduces affinity for calf thymus DNA and poly(dA-dT) oligonucleotides. Increases affinity for poly(dG-dC) oligonucleotide. Slightly reduces thermostability of protein-DNA complexes at low salt concentrations." evidence="8">
        <original>K</original>
        <variation>E</variation>
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Slightly reduces the melting temperature of the protein. Destabilizes complex with DNA. Slightly reduces affinity for calf thymus DNA and poly(dA-dT) oligonucleotides. No significant effect on affinity for poly(dG-dC) oligonucleotide. Reduces thermostability of protein-DNA complexes at low salt concentrations." evidence="8">
        <original>K</original>
        <variation>E</variation>
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Results in a significant protein misfolding, reduced thermostability, reduced ability to mediate DNA compaction and bridging. No effect on DNA binding and constraining DNA into negative supercoils." evidence="6">
        <original>F</original>
        <variation>A</variation>
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Slightly reduces the melting temperature of the protein. Slightly reduces affinity for calf thymus DNA and poly(dA-dT) oligonucleotides. No significant effect on affinity for poly(dG-dC) oligonucleotide. Reduces thermostability of protein-DNA complexes at low salt concentrations." evidence="8">
        <original>K</original>
        <variation>E</variation>
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Slightly reduces the melting temperature of the protein. Slightly reduces affinity for calf thymus DNA and poly(dA-dT) oligonucleotides. No significant effect on affinity for poly(dG-dC) oligonucleotide. Reduces thermostability of protein-DNA complexes at low salt concentrations." evidence="8">
        <original>K</original>
        <variation>E</variation>
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="8"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="17"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="23"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="36"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="turn" evidence="12">
        <location>
            <begin position="44"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="49"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P0C835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="18096617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="20512977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="35454068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="35728637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="35920727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="8">
        <source>
            <dbReference type="PubMed" id="37016575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="9"/>
    <evidence type="ECO:0007744" key="10">
        <source>
            <dbReference type="PDB" id="2JTM"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="11">
        <source>
            <dbReference type="PDB" id="3KXT"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="12">
        <source>
            <dbReference type="PDB" id="3KXT"/>
        </source>
    </evidence>
    <sequence length="60" mass="6663" checksum="665BC5BE3FA53C8D" modified="2001-10-01" version="1">MSSGKKPVKVKTPAGKEAELVPEKVWALAPKGRKGVKIGLFKDPETGKYFRHKLPDDYPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZJ0</accession>
    <name>Q97ZJ0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Signal peptidase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO0916</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90242">
        <property type="entry name" value="G90242"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992347.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZJ0"/>
    <dbReference type="STRING" id="273057.SSO0916"/>
    <dbReference type="PaxDb" id="273057-SSO0916"/>
    <dbReference type="EnsemblBacteria" id="AAK41198">
        <property type="protein sequence ID" value="AAK41198"/>
        <property type="gene ID" value="SSO0916"/>
    </dbReference>
    <dbReference type="GeneID" id="1455165"/>
    <dbReference type="KEGG" id="sso:SSO0916"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.917"/>
    <dbReference type="eggNOG" id="arCOG01739">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089996_4_1_2"/>
    <dbReference type="InParanoid" id="Q97ZJ0"/>
    <dbReference type="PhylomeDB" id="Q97ZJ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="141" mass="15782" checksum="DC4F5BB8B68EF887" modified="2001-10-01" version="1">MKMKKSDIIIILFIALIYILMFSNIVQSASVEGVSMYPIFQNGALTFYVKPISINEGNVIIYKSPYFNNYVIHRVIATDNGYYITQGVDKITNPIPDNRIGLEPASGIPKNLVVGKIVEFGNFTFSIPYLGYISILFSSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZJ6</accession>
    <name>Q97ZJ6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cell division protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Mol. Cell" volume="41" first="186" last="196">
            <title>Molecular and structural basis of ESCRT-III recruitment to membranes during archaeal cell division.</title>
            <authorList>
                <person name="Samson R.Y."/>
                <person name="Obita T."/>
                <person name="Hodgson B."/>
                <person name="Shaw M.K."/>
                <person name="Chong P.L."/>
                <person name="Williams R.L."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="21255729"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2010.12.018"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 210-259</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90242">
        <property type="entry name" value="A90242"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992338.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2XVC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.15 A"/>
        <property type="chains" value="A=210-259"/>
    </dbReference>
    <dbReference type="PDBsum" id="2XVC"/>
    <dbReference type="AlphaFoldDB" id="Q97ZJ6"/>
    <dbReference type="SMR" id="Q97ZJ6"/>
    <dbReference type="DIP" id="DIP-60243N"/>
    <dbReference type="FunCoup" id="Q97ZJ6">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="IntAct" id="Q97ZJ6">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0910"/>
    <dbReference type="PaxDb" id="273057-SSO0910"/>
    <dbReference type="EnsemblBacteria" id="AAK41192">
        <property type="protein sequence ID" value="AAK41192"/>
        <property type="gene ID" value="SSO0910"/>
    </dbReference>
    <dbReference type="GeneID" id="1455159"/>
    <dbReference type="KEGG" id="sso:SSO0910"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.912"/>
    <dbReference type="eggNOG" id="arCOG00453">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1072074_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZJ6"/>
    <dbReference type="PhylomeDB" id="Q97ZJ6"/>
    <dbReference type="EvolutionaryTrace" id="Q97ZJ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053654">
        <property type="entry name" value="Cell_Div_Complex_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041008">
        <property type="entry name" value="cell_div_CdvB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2XVC"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="29128" checksum="B99F29F89266CD81" modified="2001-10-01" version="1">MFDKLPFIFNNEKRRKAQLGKILTEISLKLKDQQTRLEEAIRRLKDRDKELFEKVVRAQVEGDDAKAKIYAQEIADIRRIIKVIYTAFLAIEKVRLKLDTVQELQGVSLVLYPVAKILGDLKDQIKGIAPEVAIALDSIISSVNGIAVETGAINDRGVVPAVVDEQARQILDEAQKMAEVKVRELLPDLPHPPVEQSSRASQAKPTIRKITERELLDYIVNNGGFLDIEHFSKVYGVEKQEVVKLLEALKNKGLIAVES</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-02-11" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZQ5</accession>
    <name>RS14Z_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein uS14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">30S ribosomal protein S14 type Z</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps14</name>
        <name evidence="5" type="synonym">rps14AB</name>
        <name type="ordered locus">SSO6391</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="function">
        <text evidence="1">Binds 16S rRNA, required for the assembly of 30S particles.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6 7 8 9 10 11 12 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1 6 7 8 9 10 11 12 13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Part of the 30S ribosomal subunit, interacts with aS34 (PubMed:39753558).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK41005" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41005.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90218">
        <property type="entry name" value="F90218"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991264.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="P=1-54"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="P=1-54"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="P=1-54"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="P=1-54"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="P=1-54"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="P=1-54"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="P=1-54"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="P=1-54"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q97ZQ5"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q97ZQ5"/>
    <dbReference type="FunCoup" id="Q97ZQ5">
        <property type="interactions" value="214"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO6391"/>
    <dbReference type="PaxDb" id="273057-SSO6391"/>
    <dbReference type="EnsemblBacteria" id="AAK41005">
        <property type="protein sequence ID" value="AAK41005"/>
        <property type="gene ID" value="SSO6391"/>
    </dbReference>
    <dbReference type="GeneID" id="1454972"/>
    <dbReference type="KEGG" id="sso:SSO6391"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.704"/>
    <dbReference type="eggNOG" id="arCOG00782">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177289_2_2_2"/>
    <dbReference type="InParanoid" id="Q97ZQ5"/>
    <dbReference type="PhylomeDB" id="Q97ZQ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.830.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="entry name" value="30s Ribosomal Protein S14, Chain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01364_A">
        <property type="entry name" value="Ribosomal_uS14_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023676">
        <property type="entry name" value="Ribosomal_uS14_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_uS14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039744">
        <property type="entry name" value="RIbosomal_uS14_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043140">
        <property type="entry name" value="Ribosomal_uS14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004424">
        <property type="entry name" value="PRK05766.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010:SF2">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00527">
        <property type="entry name" value="RIBOSOMAL_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000130999" description="Small ribosomal subunit protein uS14">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 6 7">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 6 7">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 6 7">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 6 7">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FRL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="10">
        <source>
            <dbReference type="PDB" id="9FS6"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="11">
        <source>
            <dbReference type="PDB" id="9FS8"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="12">
        <source>
            <dbReference type="PDB" id="9FSF"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="13">
        <source>
            <dbReference type="PDB" id="9FY0"/>
        </source>
    </evidence>
    <sequence length="54" mass="6404" checksum="028134B75A75E0D2" modified="2006-12-12" version="2">MGKYKPPAERKYGKGVQSCQRCGSKDSVIQKYGIYLCRQCFREVAYELGFRKYW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2026-01-28" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZR1</accession>
    <name>RS26_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small ribosomal subunit protein eS26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps26e</name>
        <name evidence="6" type="ordered locus">SSO6179</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="9 10 11" key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.90 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <scope>DISULFIDE BONDS</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Only crystallized in 30S ribosomal initiation complexes (IC) on leaderless mRNAs (lmRNA) (PubMed:39753558). It may favor translation of lmRNA (Probable) (PubMed:39753558). When present in IC on lmRNA is located near the mRNA exit channel, where it binds to the 3' end of 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 10 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 10 11">Binds 1 Zn(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S small ribosomal subunit (PubMed:39753558). When present in inititation complexes, it interacts with eS1 and uS11 (PubMed:39753558).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eukaryotic ribosomal protein eS26 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90203">
        <property type="entry name" value="F90203"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991083.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="b=1-95"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="b=1-95"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="b=1-95"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q97ZR1"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q97ZR1"/>
    <dbReference type="FunCoup" id="Q97ZR1">
        <property type="interactions" value="188"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO6179"/>
    <dbReference type="PaxDb" id="273057-SSO6179"/>
    <dbReference type="DNASU" id="1454852"/>
    <dbReference type="EnsemblBacteria" id="AAK40885">
        <property type="protein sequence ID" value="AAK40885"/>
        <property type="gene ID" value="SSO6179"/>
    </dbReference>
    <dbReference type="GeneID" id="15297948"/>
    <dbReference type="KEGG" id="sso:SSO6179"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.578"/>
    <dbReference type="eggNOG" id="arCOG04305">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129451_3_0_2"/>
    <dbReference type="InParanoid" id="Q97ZR1"/>
    <dbReference type="PhylomeDB" id="Q97ZR1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.20:FF:000004">
        <property type="entry name" value="30S ribosomal protein S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.20">
        <property type="entry name" value="Ribosomal protein S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000892">
        <property type="entry name" value="Ribosomal_eS26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038551">
        <property type="entry name" value="Ribosomal_eS26_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006816">
        <property type="entry name" value="PRK09335.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01283">
        <property type="entry name" value="Ribosomal_S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000463552" description="Small ribosomal subunit protein eS26">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9 10 11">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10 11">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="G1TFE8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FS6"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="10">
        <source>
            <dbReference type="PDB" id="9FS8"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="11">
        <source>
            <dbReference type="PDB" id="9FY0"/>
        </source>
    </evidence>
    <sequence length="95" mass="10673" checksum="FAAE7F93C46BB112" modified="2001-10-01" version="1">MPKKRENRGRRKGDKGHVGYISCDQCGARVPEDKAVCVTKMYSPVDASLASELEKKGAIIARYPVTKCYCVNCAVFLGIIKIRAENERKQKARLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q980B0</accession>
    <name>Q980B0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase (MetE-1)</fullName>
            <ecNumber evidence="2">2.1.1.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">metE-1</name>
        <name evidence="2" type="ordered locus">SSO0406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.14" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90184">
        <property type="entry name" value="H90184"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980B0"/>
    <dbReference type="FunCoup" id="Q980B0">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0406"/>
    <dbReference type="PaxDb" id="273057-SSO0406"/>
    <dbReference type="EnsemblBacteria" id="AAK40735">
        <property type="protein sequence ID" value="AAK40735"/>
        <property type="gene ID" value="SSO0406"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0406"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.402"/>
    <dbReference type="eggNOG" id="arCOG01877">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040013_3_1_2"/>
    <dbReference type="InParanoid" id="Q980B0"/>
    <dbReference type="PhylomeDB" id="Q980B0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013215">
        <property type="entry name" value="Cbl-indep_Met_Synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006348">
        <property type="entry name" value="PRK08575.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006349">
        <property type="entry name" value="PRK08575.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30519">
        <property type="entry name" value="5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08267">
        <property type="entry name" value="Meth_synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cobalamin-independent methionine synthase MetE N-terminal" evidence="1">
        <location>
            <begin position="85"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="330" mass="38448" checksum="D9B9F8914C051F4B" modified="2001-10-01" version="1">MIMNVKKALVGSFPKNPKLGKVISWYNTGKINKEKLEKHISENIKKLFELAGNIKLEYTTNGLFRWDDIVDVTFGFISGTEKGPLQRFFDNNFYYRQPIIKEKINVKKREENSFLQDLEFSRKIKEEVSLPSKLKAVIPGPLTYYVLSDNRYYNSPIELMMDYASVVNSLSQELSSVVDAIEIHEPAIYTNNIKRDILEKLPEIYKSMLDNVKIEKHLITYFEINNLKRLDTLFSLPVDYFGIDVIENLKKLGRVYTYFSSRKVYLGMLNARNTKMERITTIIRVYNSVKRKGVSDVIIGNNTLFDFIPEVVVVKKLKLLKKLEKVEINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q980H3</accession>
    <name>Q980H3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH dehydrogenase subunit D (NuoD)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NuoD</name>
        <name evidence="7" type="ordered locus">SSO0324</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90175">
        <property type="entry name" value="D90175"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990617.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980H3"/>
    <dbReference type="SMR" id="Q980H3"/>
    <dbReference type="FunCoup" id="Q980H3">
        <property type="interactions" value="131"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0324"/>
    <dbReference type="PaxDb" id="273057-SSO0324"/>
    <dbReference type="EnsemblBacteria" id="AAK40659">
        <property type="protein sequence ID" value="AAK40659"/>
        <property type="gene ID" value="SSO0324"/>
    </dbReference>
    <dbReference type="GeneID" id="1455469"/>
    <dbReference type="KEGG" id="sso:SSO0324"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.318"/>
    <dbReference type="eggNOG" id="arCOG01548">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_1_2_2"/>
    <dbReference type="InParanoid" id="Q980H3"/>
    <dbReference type="PhylomeDB" id="Q980H3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014029">
        <property type="entry name" value="NADH_UbQ_OxRdtase_49kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004739">
        <property type="entry name" value="PRK06075.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 49 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00535">
        <property type="entry name" value="COMPLEX1_49K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="6">
        <location>
            <begin position="141"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="410" mass="46774" checksum="C7E9C31EBE2CCE95" modified="2001-10-01" version="1">MTSQPQNEIQEQLLAATGMSVEFIPVQGELNVGPQHPGSGHMRIFVKLNGDIIEDVDLDVGYVHRAVEKLSENRNYMHLIPLVERPAILDSIHMNLGYIIAVEKILGVDVPERAQYLRSFAAEVNRIASHLYGLGILAIFLGHSTGFMWGFGDREVWVTILEALTGARVTNSYVIPGGVRKDITQEIKEMTIKAIAYQRKRLEDWKKIFFYNPSIRARLENVGVMSRENAIKWGAVGPNLRASGVYYDVRKIEPYGAYDKLDFEIPVYKEGDGLARGLVRLEEIEQSMRILEQIIKNIPEGNILSDRFFKQIPPIRLKKYWETYRRIVLPGYYASFRPPKGEAISRVEAGRGELAYYVVSDGSPKPYRLRMITPSYRLIYVFKELCKGSRYADLVSIYGSLDYFPPEADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q980M6</accession>
    <name>Q980M6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0265</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90168">
        <property type="entry name" value="E90168"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990537.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980M6"/>
    <dbReference type="FunCoup" id="Q980M6">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0265"/>
    <dbReference type="REBASE" id="5091">
        <property type="entry name" value="M.SsfORF265P"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO0265"/>
    <dbReference type="EnsemblBacteria" id="AAK40604">
        <property type="protein sequence ID" value="AAK40604"/>
        <property type="gene ID" value="SSO0265"/>
    </dbReference>
    <dbReference type="GeneID" id="1455414"/>
    <dbReference type="KEGG" id="sso:SSO0265"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.260"/>
    <dbReference type="eggNOG" id="arCOG04157">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006958_2_2_2"/>
    <dbReference type="InParanoid" id="Q980M6"/>
    <dbReference type="PhylomeDB" id="Q980M6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00675">
        <property type="entry name" value="dcm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629">
        <property type="entry name" value="CYTOSINE-SPECIFIC METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629:SF52">
        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="325" mass="36664" checksum="D4E08633DFDA36D9" modified="2001-10-01" version="1">MELIKIIDLFSGAGGFSLGFKKLGIEPKLAIDINHAATRTYSLNFPNTIVIEDDIREISGGEILKNVGNDIDVVIGGPPCEGYTAANPLRMQDPLDRLYLDQRGNLTLEFIRIVDEVKPKIFVMENVPAIIETQSLRDALINEFKKAGYGNIFFNILHAEDYGNPSKRSRVFISNVRISPIKNQKRITVYDAIHDLDNKLDVPNNEVSEPNEKKLLKISKIGYGKYLTMYRASKGRSIPLYIRLSPFDLAPTILGNSRFIHPFHDRFLTVREQARLMSYPDDHVFLGSKDEQYNQVGESVPVALSTAIAKEILGILNERTIFRPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q980M9</accession>
    <name>Q980M9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0261</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90168">
        <property type="entry name" value="B90168"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990533.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980M9"/>
    <dbReference type="STRING" id="273057.SSO0261"/>
    <dbReference type="PaxDb" id="273057-SSO0261"/>
    <dbReference type="EnsemblBacteria" id="AAK40601">
        <property type="protein sequence ID" value="AAK40601"/>
        <property type="gene ID" value="SSO0261"/>
    </dbReference>
    <dbReference type="GeneID" id="1455411"/>
    <dbReference type="KEGG" id="sso:SSO0261"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.258"/>
    <dbReference type="eggNOG" id="arCOG01120">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2044517_0_0_2"/>
    <dbReference type="InParanoid" id="Q980M9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="32"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="119" mass="14081" checksum="616B7B17372BE291" modified="2001-10-01" version="1">MSSNQTNRLLLKARRVVKEGRFVSLTLNDALFRIFIYIGRNNDYILNLNFCSCSFYIFNVVLRGKYDFCYHVLGLKYALEKDQITKIELYADDFKEILTEIYAYGKSLKLRRILNKVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="144" xmlns="https://uniprot.org/uniprot">
    <accession>Q980P5</accession>
    <name>Q980P5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Adenylosuccinate synthetase</fullName>
            <shortName evidence="8">AMPSase</shortName>
            <shortName evidence="8">AdSS</shortName>
            <ecNumber evidence="8 9">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">purA</name>
        <name evidence="10" type="ordered locus">SSO0242</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 9">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="8 9"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90165">
        <property type="entry name" value="H90165"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990500.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980P5"/>
    <dbReference type="FunCoup" id="Q980P5">
        <property type="interactions" value="346"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0242"/>
    <dbReference type="PaxDb" id="273057-SSO0242"/>
    <dbReference type="EnsemblBacteria" id="AAK40583">
        <property type="protein sequence ID" value="AAK40583"/>
        <property type="gene ID" value="SSO0242"/>
    </dbReference>
    <dbReference type="GeneID" id="1455393"/>
    <dbReference type="KEGG" id="sso:SSO0242"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.238"/>
    <dbReference type="eggNOG" id="arCOG04387">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029848_0_0_2"/>
    <dbReference type="InParanoid" id="Q980P5"/>
    <dbReference type="PhylomeDB" id="Q980P5"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
        <property type="term" value="P:IMP metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.440.10:FF:000007">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.170.10:FF:000005">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003295">
        <property type="entry name" value="PRK04293.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF0">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="11"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="40"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="247"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="279"/>
            <end position="281"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="319"/>
            <end position="321"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK40583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="335" mass="37440" checksum="C01E75900BEF1918" modified="2001-10-01" version="1">MLELIIGGFFGDEGKGKVAAYLGIKDRPKLSVRTGSINAGHTVTYSGRIWKLRIIPSAFINKSTELALGPGALTSLDALFKEMRETETYDRLYIDSHVGIITEDEIRDEKNDPYLMNVIGSTGQGVGYAESKRILRKLKLAKDYKELEKFIIDVPNKILDYLDNGHNILIEGTQGHYLSLYHGEYPYVTSRNTTASGVLSEAGIGPKYVDHIIITFKSYVTRVGKGPLEGELTEEDAKKLGLTEYGTVTGRLRRVAPFNVKLAREAIRINSATIVAITKLDTLFKDAYRVREYEKLPAEARKWLDELEEELKTPIGLIGTGEDAYDMIDLREEVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q980W8</accession>
    <name>LYSW_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Alpha-aminoadipate/glutamate carrier protein LysW</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>AAA carrier protein LysW</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">lysW</name>
        <name type="ordered locus">SSO5317</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="29537" last="29549">
            <title>The Sulfolobus solfataricus Lrp-like protein LysM regulates lysine biosynthesis in response to lysine availability.</title>
            <authorList>
                <person name="Brinkman A.B."/>
                <person name="Bell S.D."/>
                <person name="Lebbink R.J."/>
                <person name="de Vos W.M."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="12042311"/>
            <dbReference type="DOI" id="10.1074/jbc.M203528200"/>
        </citation>
        <scope>FUNCTION IN LYSINE BIOSYNTHESIS</scope>
        <scope>INDUCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3">Carrier protein that bears the covalently bound substrates for arginine and lysine biosynthesis; bound L-glutamate is sequentially converted to L-ornithine, while bound alpha-aminoadipate (AAA) is sequentially converted to L-lysine.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Formation of an isopeptide bond between the gamma-carboxyl group of the C-terminal glutamate and the amino group of alpha-aminoadipate (AAA) is catalyzed by LysX. The bound AAA is then converted to L-lysine in a series of reactions catalyzed by LysZ, LysY and LysJ. Release of the product L-lysine is catalyzed by LysK. Formation of an isopeptide bond between the gamma-carboxyl group of the C-terminal glutamate and the amino group of L-glutamate is catalyzed by ArgX. The bound substrate is then sequentially converted to ornithine which is eventually converted to L-arginine.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BK000545">
        <property type="protein sequence ID" value="DAA00052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90156">
        <property type="entry name" value="A90156"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990383.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980W8"/>
    <dbReference type="SMR" id="Q980W8"/>
    <dbReference type="STRING" id="273057.SSO5317"/>
    <dbReference type="PaxDb" id="273057-SSO5317"/>
    <dbReference type="EnsemblBacteria" id="AAK40504">
        <property type="protein sequence ID" value="AAK40504"/>
        <property type="gene ID" value="SSO5317"/>
    </dbReference>
    <dbReference type="GeneID" id="42362152"/>
    <dbReference type="KEGG" id="sso:SSO5317"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.155"/>
    <dbReference type="eggNOG" id="arCOG01588">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_195720_1_0_2"/>
    <dbReference type="InParanoid" id="Q980W8"/>
    <dbReference type="PhylomeDB" id="Q980W8"/>
    <dbReference type="UniPathway" id="UPA00033"/>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd13946">
        <property type="entry name" value="LysW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005906">
        <property type="entry name" value="LysW"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041070">
        <property type="entry name" value="carrier_LysW_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40393:SF2">
        <property type="entry name" value="ALPHA-AMINOADIPATE_GLUTAMATE CARRIER PROTEIN LYSW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40393">
        <property type="entry name" value="LYSINE BIOSYNTHESIS PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21344">
        <property type="entry name" value="Zn_ribbon_LysW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-1017">Isopeptide bond</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000084537" description="Alpha-aminoadipate/glutamate carrier protein LysW">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TFIIB-type">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="EDWGE">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="5-glutamyl 2-aminoadipic acid; alternate" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="modified residue" description="5-glutamyl glutamate; alternate" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="modified residue" description="5-glutamyl N2-lysine; alternate" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="modified residue" description="5-glutamyl N2-ornithine; alternate" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q976J8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12042311"/>
        </source>
    </evidence>
    <sequence length="56" mass="6065" checksum="59C8A2C54D96C676" modified="2001-10-01" version="1">MVNLKCPICGGEITVEDDALPGELVEHECGAQLEVVKQNGKLSLRLAEQIGEDWGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2026-01-28" version="139" xmlns="https://uniprot.org/uniprot">
    <accession>Q980X1</accession>
    <accession>Q7SI96</accession>
    <name>LYSY_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">[LysW]-L-2-aminoadipate/[LysW]-L-glutamate phosphate reductase</fullName>
            <ecNumber evidence="1">1.2.1.103</ecNumber>
            <ecNumber evidence="1">1.2.1.106</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">lysY</name>
        <name type="synonym">argC</name>
        <name type="ordered locus">SSO0155</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="29537" last="29549">
            <title>The Sulfolobus solfataricus Lrp-like protein LysM regulates lysine biosynthesis in response to lysine availability.</title>
            <authorList>
                <person name="Brinkman A.B."/>
                <person name="Bell S.D."/>
                <person name="Lebbink R.J."/>
                <person name="de Vos W.M."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="12042311"/>
            <dbReference type="DOI" id="10.1074/jbc.M203528200"/>
        </citation>
        <scope>INVOLVEMENT IN LYSINE BIOSYNTHESIS</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in both the arginine and lysine biosynthetic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[amino-group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + phosphate + NADP(+) = [amino-group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9712"/>
            <dbReference type="Rhea" id="RHEA-COMP:9714"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78499"/>
            <dbReference type="ChEBI" id="CHEBI:78501"/>
            <dbReference type="EC" id="1.2.1.103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-glutamyl-5-semialdehyde)-L-glutamate + phosphate + NADP(+) = [amino-group carrier protein]-C-terminal-gamma-(5-phospho-L-glutamyl)-L-glutamate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52668"/>
            <dbReference type="Rhea" id="RHEA-COMP:13313"/>
            <dbReference type="Rhea" id="RHEA-COMP:13327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:136717"/>
            <dbReference type="ChEBI" id="CHEBI:136761"/>
            <dbReference type="EC" id="1.2.1.106"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 3/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAGSA dehydrogenase family. Type 1 subfamily. LysY sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.103" evidence="1"/>
    <dbReference type="EC" id="1.2.1.106" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BK000545">
        <property type="protein sequence ID" value="DAA00049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90155">
        <property type="entry name" value="F90155"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990380.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980X1"/>
    <dbReference type="SMR" id="Q980X1"/>
    <dbReference type="FunCoup" id="Q980X1">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0155"/>
    <dbReference type="PaxDb" id="273057-SSO0155"/>
    <dbReference type="EnsemblBacteria" id="AAK40501">
        <property type="protein sequence ID" value="AAK40501"/>
        <property type="gene ID" value="SSO0155"/>
    </dbReference>
    <dbReference type="GeneID" id="1453328"/>
    <dbReference type="GeneID" id="44129117"/>
    <dbReference type="KEGG" id="sso:SSO0155"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.152"/>
    <dbReference type="eggNOG" id="arCOG00495">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006384_0_1_2"/>
    <dbReference type="InParanoid" id="Q980X1"/>
    <dbReference type="PhylomeDB" id="Q980X1"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00037"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043870">
        <property type="term" value="F:N-acetyl-gamma-aminoadipyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23939">
        <property type="entry name" value="AGPR_1_C_LysY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17895">
        <property type="entry name" value="AGPR_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00150">
        <property type="entry name" value="ArgC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02083">
        <property type="entry name" value="LysY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050085">
        <property type="entry name" value="AGPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023013">
        <property type="entry name" value="AGPR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058924">
        <property type="entry name" value="AGPR_dimerisation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000706">
        <property type="entry name" value="AGPR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037535">
        <property type="entry name" value="LysY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01850">
        <property type="entry name" value="argC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32338:SF11">
        <property type="entry name" value="[LYSW]-L-2-AMINOADIPATE_[LYSW]-L-GLUTAMATE PHOSPHATE REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32338">
        <property type="entry name" value="N-ACETYL-GAMMA-GLUTAMYL-PHOSPHATE REDUCTASE, CHLOROPLASTIC-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22698">
        <property type="entry name" value="Semialdhyde_dhC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01224">
        <property type="entry name" value="ARGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000112498" description="[LysW]-L-2-aminoadipate/[LysW]-L-glutamate phosphate reductase">
        <location>
            <begin position="1"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="13"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="12042311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3">
        <source>
            <dbReference type="PubMed" id="12042311"/>
        </source>
    </evidence>
    <sequence length="352" mass="38685" checksum="CAA3053D6E08A188" modified="2001-10-01" version="1">MKDKVRVAVVGGSGYTGGELLRILVTHPKTEISVITSREYAGKPVSLIHPNLRGLISLNFTNFSIDKISDKADAIFLALPHGVSLNYAPKLLDLGLTVVDLSADFRLKNPELYKIWYNYEHPYPDLLDKAVYGLPELHYEELKNAKLIASPGCNATATILALAPIVATKITDEKKFISDVKVGSSEGGAKPSEGSHHPERQNAIRPYEAEGHRHAAEAEQELSRIAKANISVSIVPHAVSSIRGALASAHTWLTNELEEIEIWKKIAEFYRGKRFIRIIRGNIHPYPDPKFVIGSNFADIGFAVEKRISRLTTFSAIDNLMKGAAGQAVQAFNISMGFNEDDGLKLVPLRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Y6</accession>
    <name>Q980Y6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90153">
        <property type="entry name" value="G90153"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922894.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Y6"/>
    <dbReference type="PaxDb" id="273057-SSO0133"/>
    <dbReference type="EnsemblBacteria" id="AAK40486">
        <property type="protein sequence ID" value="AAK40486"/>
        <property type="gene ID" value="SSO0133"/>
    </dbReference>
    <dbReference type="GeneID" id="1453313"/>
    <dbReference type="KEGG" id="sso:SSO0133"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.127"/>
    <dbReference type="eggNOG" id="arCOG06508">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134214_0_0_2"/>
    <dbReference type="InParanoid" id="Q980Y6"/>
    <dbReference type="PhylomeDB" id="Q980Y6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase InsH N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="142" mass="16556" checksum="5367B3A411338AAB" modified="2001-10-01" version="1">MVKAISTEKDLLLKVDKSFPWETFRSKLKSLYSKKPKWNVISLLKVLLIKLIFDISWNNLEGEIRDSKRFMDFLGGKIPPKSTVFSFYKKLQQTVIQEGETMRTTLMDELNKALDKVISEYREKGFELEVGREKTIGSRTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Z6</accession>
    <name>Q980Z6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0121</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90152">
        <property type="entry name" value="E90152"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Z6"/>
    <dbReference type="STRING" id="273057.SSO0121"/>
    <dbReference type="PaxDb" id="273057-SSO0121"/>
    <dbReference type="EnsemblBacteria" id="AAK40476">
        <property type="protein sequence ID" value="AAK40476"/>
        <property type="gene ID" value="SSO0121"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0121"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.116"/>
    <dbReference type="eggNOG" id="arCOG05986">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_424309_0_0_2"/>
    <dbReference type="InParanoid" id="Q980Z6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF046072">
        <property type="entry name" value="UpsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="695" mass="79966" checksum="8835FE8AE97A5D0A" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="137" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX84</accession>
    <name>SECY_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein translocase subunit SecY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Protein transport protein SEC61 subunit alpha homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">secY</name>
        <name type="ordered locus">SSO0695</name>
        <name type="ORF">C10_035</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the Sec protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90217">
        <property type="entry name" value="E90217"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991249.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX84"/>
    <dbReference type="SMR" id="Q9UX84"/>
    <dbReference type="FunCoup" id="Q9UX84">
        <property type="interactions" value="262"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0695"/>
    <dbReference type="PaxDb" id="273057-SSO0695"/>
    <dbReference type="EnsemblBacteria" id="AAK40996">
        <property type="protein sequence ID" value="AAK40996"/>
        <property type="gene ID" value="SSO0695"/>
    </dbReference>
    <dbReference type="GeneID" id="1454963"/>
    <dbReference type="GeneID" id="44129694"/>
    <dbReference type="KEGG" id="sso:SSO0695"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.695"/>
    <dbReference type="eggNOG" id="arCOG04169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031763_3_0_2"/>
    <dbReference type="InParanoid" id="Q9UX84"/>
    <dbReference type="PhylomeDB" id="Q9UX84"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005048">
        <property type="term" value="F:signal sequence binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01465">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026593">
        <property type="entry name" value="SecY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006341">
        <property type="entry name" value="PRK08568.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000131771" description="Protein translocase subunit SecY">
        <location>
            <begin position="1"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=Helix 1" evidence="2">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Discontinuously helical; Name=Helix 2" evidence="1">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="intramembrane region" description="Helical; Name=Helix 2A" evidence="1">
        <location>
            <begin position="60"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="intramembrane region" evidence="1">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="intramembrane region" description="Helical; Name=Helix 2B" evidence="1">
        <location>
            <begin position="80"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=Helix 3" evidence="2">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=Helix 4" evidence="2">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="172"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=Helix 5" evidence="2">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="198"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=Helix 6" evidence="2">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="263"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=Helix 7" evidence="2">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="310"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=Helix 8" evidence="2">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="368"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=Helix 9" evidence="2">
        <location>
            <begin position="411"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="430"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=Helix 10" evidence="2">
        <location>
            <begin position="433"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
    <sequence length="469" mass="50493" checksum="D6AF49989F034EF8" modified="2000-05-01" version="1">MSFIDSLATLGQYLPAVTKPKEKPSLGQKLVWSLVAVIIYLIMASTPLYGITSASFFKNLILEQIIFASTTGTLAQLGIGPIITAGLIMQILAGSKLISIDLNDPDDRVKFTEAQKGLAFIFILVESALFGYVLARTSTTINASILFIAGIVIAQLIVATYLILLLDELIQKGWGLGSGVSLFILAGVMKIMFWDMFGIASVSSQNLPIGFFPALFTALASHSDVLNLIVNTSTKNLFQPDLVGLVTTIALIIITIYLTTMTIEIPVTSQKLRGIRRTIPLNFLYVSSIPVIFVAVLGSDIQLFASLASYVSPSASNILNTVSGVFFFPPPNSAIPHSIYAVVLDPLGALEYAVVFIVLSILFGILWVDVAGLDPATQAQQLVEAGIEIPGVRNNPKIIEGILARYIYPLAFFSSIIVGLIAVFATLLGAYGTGIGILLAVTIAIQYYSLLAYERSLEMYPLLKRLIGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXA2</accession>
    <name>RL22_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L22</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl22</name>
        <name evidence="1" type="synonym">rpl22Ab</name>
        <name type="ordered locus">SSO0713</name>
        <name type="ORF">C10_017</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds specifically to 23S rRNA. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90219">
        <property type="entry name" value="F90219"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923053.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXA2"/>
    <dbReference type="SMR" id="Q9UXA2"/>
    <dbReference type="FunCoup" id="Q9UXA2">
        <property type="interactions" value="217"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0713"/>
    <dbReference type="PaxDb" id="273057-SSO0713"/>
    <dbReference type="EnsemblBacteria" id="AAK41013">
        <property type="protein sequence ID" value="AAK41013"/>
        <property type="gene ID" value="SSO0713"/>
    </dbReference>
    <dbReference type="GeneID" id="1454980"/>
    <dbReference type="KEGG" id="sso:SSO0713"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.713"/>
    <dbReference type="eggNOG" id="arCOG04098">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083987_0_2_2"/>
    <dbReference type="InParanoid" id="Q9UXA2"/>
    <dbReference type="PhylomeDB" id="Q9UXA2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000015">
        <property type="entry name" value="50S ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_A">
        <property type="entry name" value="Ribosomal_uL22_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057265">
        <property type="entry name" value="Ribosomal_uL22_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_uL22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003260">
        <property type="entry name" value="PRK04223.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000125288" description="Large ribosomal subunit protein uL22">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAB57590." evidence="2" ref="1">
        <original>E</original>
        <variation>A</variation>
        <location>
            <position position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="157" mass="18126" checksum="D5AFDBC37DA41664" modified="2001-06-01" version="2">MASWKYPQLDVDELKIAKAVIRDVPESIRDLYNVCKAIRGMYLNDAKDFLNRVLEEKEALPFWRYNKGASHKSNISAKWKIKAGRYPKKAIRQVLKVLENAEANATNKGLDVDKLIIRHIAAHKGITLQRYMPRAFGRATAKYRRTSNIEVILEEVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXC7</accession>
    <name>RNP2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease P protein component 2</fullName>
            <shortName evidence="1">RNase P component 2</shortName>
            <ecNumber evidence="1">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pop5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnp2</name>
        <name type="ordered locus">SSO0739</name>
        <name type="ORF">C20_030</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90222">
        <property type="entry name" value="D90222"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991313.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXC7"/>
    <dbReference type="SMR" id="Q9UXC7"/>
    <dbReference type="STRING" id="273057.SSO0739"/>
    <dbReference type="PaxDb" id="273057-SSO0739"/>
    <dbReference type="EnsemblBacteria" id="AAK41035">
        <property type="protein sequence ID" value="AAK41035"/>
        <property type="gene ID" value="SSO0739"/>
    </dbReference>
    <dbReference type="GeneID" id="1455002"/>
    <dbReference type="KEGG" id="sso:SSO0739"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.735"/>
    <dbReference type="eggNOG" id="arCOG01365">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1801579_0_0_2"/>
    <dbReference type="InParanoid" id="Q9UXC7"/>
    <dbReference type="PhylomeDB" id="Q9UXC7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00755">
        <property type="entry name" value="RNase_P_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000140029" description="Ribonuclease P protein component 2">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00755"/>
        </source>
    </evidence>
    <sequence length="143" mass="16523" checksum="75324B5EB1647ABA" modified="2000-05-01" version="1">MNSIQLIIDIILILWILILTVLYFKRRQLNVDIVKNKKIIRAKRYIVFYVISEYKVKGDDLERVIRNSLKDLLGSVWLNIANPKVVTYREDTQEGIISTNRVGYKAVLASLPFAKEINNNKILIVPRRTTGSLKKAKKLIGLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2025-06-18" version="152" xmlns="https://uniprot.org/uniprot">
    <accession>O08424</accession>
    <accession>Q9UWT6</accession>
    <name>HMDH_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>3-hydroxy-3-methylglutaryl-coenzyme A reductase</fullName>
            <shortName>HMG-CoA reductase</shortName>
            <ecNumber>1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hmgA</name>
        <name type="ordered locus">SSO0531</name>
        <name type="ORF">C22_020</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="J. Bacteriol." volume="179" first="3632" last="3638">
            <title>3-hydroxy-3-methylglutaryl coenzyme A reductase of Sulfolobus solfataricus: DNA sequence, phylogeny, expression in Escherichia coli of the hmgA gene, and purification and kinetic characterization of the gene product.</title>
            <authorList>
                <person name="Bochar D.A."/>
                <person name="Brown J.R."/>
                <person name="Doolittle W.F."/>
                <person name="Klenk H.-P."/>
                <person name="Lam W."/>
                <person name="Schenk M.E."/>
                <person name="Stauffacher C.V."/>
                <person name="Rodwell V.W."/>
            </authorList>
            <dbReference type="PubMed" id="9171410"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Converts HMG-CoA to mevalonate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(R)-mevalonate + 2 NADP(+) + CoA = (3S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15989"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.34"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text>Optimum pH is 5.5.</text>
        </phDependence>
        <temperatureDependence>
            <text>Optimum temperature is 85 degrees Celsius. Stable at 90 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34"/>
    <dbReference type="EMBL" id="U95360">
        <property type="protein sequence ID" value="AAC45370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90199">
        <property type="entry name" value="D90199"/>
    </dbReference>
    <dbReference type="PIR" id="T51306">
        <property type="entry name" value="T51306"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923027.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O08424"/>
    <dbReference type="SMR" id="O08424"/>
    <dbReference type="FunCoup" id="O08424">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0531"/>
    <dbReference type="PaxDb" id="273057-SSO0531"/>
    <dbReference type="EnsemblBacteria" id="AAK40851">
        <property type="protein sequence ID" value="AAK40851"/>
        <property type="gene ID" value="SSO0531"/>
    </dbReference>
    <dbReference type="GeneID" id="1454818"/>
    <dbReference type="GeneID" id="44129547"/>
    <dbReference type="KEGG" id="sso:SSO0531"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.530"/>
    <dbReference type="eggNOG" id="arCOG04260">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001734_2_2_2"/>
    <dbReference type="InParanoid" id="O08424"/>
    <dbReference type="PhylomeDB" id="O08424"/>
    <dbReference type="BioCyc" id="MetaCyc:MONOMER-14624"/>
    <dbReference type="UniPathway" id="UPA00058">
        <property type="reaction ID" value="UER00103"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00643">
        <property type="entry name" value="HMG-CoA_reductase_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.420:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004554">
        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00533">
        <property type="entry name" value="HMG_CoA_R_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF24">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00066">
        <property type="entry name" value="HMG_COA_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00318">
        <property type="entry name" value="HMG_COA_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000114465" description="3-hydroxy-3-methylglutaryl-coenzyme A reductase">
        <location>
            <begin position="1"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAC45370." evidence="2" ref="1">
        <original>V</original>
        <variation>L</variation>
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="409" mass="44009" checksum="8D1FA64E117CFF46" modified="2001-06-20" version="2">MKIDEVVEKLVKGEISFHEVDNLLEANAAMVARRLALEKIVGVGLPSIGSTVIDYSEIKNKNAENVIGAIQIPLGIVGPIRVNGDYAKGDFYVPMATTEGALIASVNRGIKAVTLSGGVRAKVLKDEMTRAPVFKFDSIEQIPNFLKFIEENLEKIRNIANSTSHHGKLKSITPFVLGNNVWLRFSFETGDAMGMNMVTIAVEKVCEFIEENFPSADCLAVSGNMCSDKKQTNVNSLFGRGKTVVAEALIKKDVIRNILHSNAQLIHDINLRKNWLGTARAGSLSQFNAHFANIVTAIFIATGQDVAQIVESSSGYTWTEVRGEDLYISVTLPSLEVGTVGGGTRLPTQKEALSIMGVYGSGNPPGSNAKKLAEIIASTVLSGELNLLAALSNKELGKAHAKLGRAMKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1991-08-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>P22795</accession>
    <accession>Q9P998</accession>
    <name>Y3021_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein SSO3021</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO3021</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="J. Biol. Chem." volume="275" first="22082" last="22089">
            <title>Identification and molecular characterization of the first alpha-xylosidase from an Archaeon.</title>
            <authorList>
                <person name="Moracci M."/>
                <person name="Ponzano B.C."/>
                <person name="Trincone A."/>
                <person name="Fusco S."/>
                <person name="De Rosa M."/>
                <person name="van der Oost J."/>
                <person name="Sensen C.W."/>
                <person name="Charlebois R.L."/>
                <person name="Rossi M."/>
            </authorList>
            <dbReference type="PubMed" id="10801892"/>
            <dbReference type="DOI" id="10.1074/jbc.M910392199"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1990" name="Gene" volume="94" first="89" last="94">
            <title>Isolation and sequencing of a new beta-galactosidase-encoding archaebacterial gene.</title>
            <authorList>
                <person name="Cubellis M.V."/>
                <person name="Rozzo C."/>
                <person name="Montecucchi P."/>
                <person name="Rossi M."/>
            </authorList>
            <dbReference type="PubMed" id="2121622"/>
            <dbReference type="DOI" id="10.1016/0378-1119(90)90472-4"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 377-592</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK43122" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="AJ251975">
        <property type="protein sequence ID" value="CAB99207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43122.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="M34696">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90483">
        <property type="entry name" value="C90483"/>
    </dbReference>
    <dbReference type="PIR" id="JQ0768">
        <property type="entry name" value="JQ0768"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992678.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P22795"/>
    <dbReference type="SMR" id="P22795"/>
    <dbReference type="STRING" id="273057.SSO3021"/>
    <dbReference type="PaxDb" id="273057-SSO3021"/>
    <dbReference type="EnsemblBacteria" id="AAK43122">
        <property type="protein sequence ID" value="AAK43122"/>
        <property type="gene ID" value="SSO3021"/>
    </dbReference>
    <dbReference type="GeneID" id="1453041"/>
    <dbReference type="KEGG" id="sso:SSO3021"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3114"/>
    <dbReference type="eggNOG" id="arCOG07336">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_499426_0_0_2"/>
    <dbReference type="InParanoid" id="P22795"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000206882" description="Uncharacterized protein SSO3021">
        <location>
            <begin position="1"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="592" mass="68145" checksum="31735F22BD371475" modified="2001-06-20" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-20" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>P58191</accession>
    <name>SECE_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein translocase subunit SecE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Protein transport protein Sec61 gamma subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">secE</name>
        <name type="ordered locus">SSO5663</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY (alpha), SecG (beta) and SecE (gamma) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90177">
        <property type="entry name" value="F90177"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990639.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58191"/>
    <dbReference type="SMR" id="P58191"/>
    <dbReference type="STRING" id="273057.SSO5663"/>
    <dbReference type="PaxDb" id="273057-SSO5663"/>
    <dbReference type="EnsemblBacteria" id="AAK40677">
        <property type="protein sequence ID" value="AAK40677"/>
        <property type="gene ID" value="SSO5663"/>
    </dbReference>
    <dbReference type="GeneID" id="1455487"/>
    <dbReference type="KEGG" id="sso:SSO5663"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.338"/>
    <dbReference type="eggNOG" id="arCOG02204">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191921_1_0_2"/>
    <dbReference type="InParanoid" id="P58191"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006906">
        <property type="entry name" value="PRK09400.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000104229" description="Protein translocase subunit SecE">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00422"/>
        </source>
    </evidence>
    <sequence length="62" mass="7184" checksum="CD51874F424BE9FA" modified="2001-06-20" version="1">MSSNKIINWFRRLREDWNRIITVARKPDRNFFSLNLKVTLLVLAVVGVLAYIIQLALTLIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX89</accession>
    <name>Q7LX89_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74019">
        <property type="entry name" value="S74019"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990134.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX89"/>
    <dbReference type="STRING" id="273057.SSO2107"/>
    <dbReference type="PaxDb" id="273057-SSO2107"/>
    <dbReference type="EnsemblBacteria" id="AAK42286">
        <property type="protein sequence ID" value="AAK42286"/>
        <property type="gene ID" value="SSO2107"/>
    </dbReference>
    <dbReference type="GeneID" id="1453613"/>
    <dbReference type="KEGG" id="sso:SSO2107"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2193"/>
    <dbReference type="eggNOG" id="arCOG01205">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2204258_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX89"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37030">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37030:SF3">
        <property type="entry name" value="POLYMERASE NUCLEOTIDYL TRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="12"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="106" mass="12470" checksum="81C65D4CF0756365" modified="2011-05-31" version="1">MEPIEKIKLLRNWREALRKLNLERFEEVYVFGSVVSDKITGASDIDLILVIKRGEDRNKALIGFFDEVESKLGEKASYLFDVKVIYEDEKELPPYKFFLRDAVRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXA2</accession>
    <name>Q7LXA2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbon monoxide dehydrogenase, large chain (CutA-2)</fullName>
            <ecNumber evidence="4">1.2.7.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cutA-2</name>
        <name evidence="4" type="ordered locus">SSO2091</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.4" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73085">
        <property type="entry name" value="S73085"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989792.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXA2"/>
    <dbReference type="FunCoup" id="Q7LXA2">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2091"/>
    <dbReference type="PaxDb" id="273057-SSO2091"/>
    <dbReference type="EnsemblBacteria" id="AAK42270">
        <property type="protein sequence ID" value="AAK42270"/>
        <property type="gene ID" value="SSO2091"/>
    </dbReference>
    <dbReference type="GeneID" id="1453597"/>
    <dbReference type="KEGG" id="sso:SSO2091"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2169"/>
    <dbReference type="eggNOG" id="arCOG01167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_2_0_2"/>
    <dbReference type="InParanoid" id="Q7LXA2"/>
    <dbReference type="PhylomeDB" id="Q7LXA2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:anaerobic carbon-monoxide dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.50">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.365.10">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046867">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908:SF132">
        <property type="entry name" value="ALDEHYDE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="MoCoBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20256">
        <property type="entry name" value="MoCoBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde oxidase/xanthine dehydrogenase a/b hammerhead" evidence="3">
        <location>
            <begin position="11"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="698" mass="76117" checksum="14D816776AF1D2C5" modified="2011-05-31" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXF5</accession>
    <name>Q7LXF5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0822</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90232">
        <property type="entry name" value="G90232"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923113.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXF5"/>
    <dbReference type="SMR" id="Q7LXF5"/>
    <dbReference type="STRING" id="273057.SSO0822"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO0822"/>
    <dbReference type="DNASU" id="1455085"/>
    <dbReference type="EnsemblBacteria" id="AAK41118">
        <property type="protein sequence ID" value="AAK41118"/>
        <property type="gene ID" value="SSO0822"/>
    </dbReference>
    <dbReference type="GeneID" id="71027418"/>
    <dbReference type="KEGG" id="sso:SSO0822"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.834"/>
    <dbReference type="eggNOG" id="arCOG01417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1465184_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXF5"/>
    <dbReference type="PhylomeDB" id="Q7LXF5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="27"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="184" mass="21385" checksum="03AE964F72E35078" modified="2011-05-31" version="1">MFFARLIPLKGVLELPFIVKEVITMSGYKELKIVVMGKFPDDNLKAIFFEIVKRLQLEDNIIYKGYLTSREELFNMVSEARCMIYPTHEDSFSLAILESITVGTPVVAYDIPGPKSVYSGLSAVKFVEEYNIKLMATEVTKILTMNDDEYNSLIFNDKMDKFIEKHTDWDLVVERYYRDLMSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2023-11-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXH3</accession>
    <name>Q7LXH3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90230">
        <property type="entry name" value="C90230"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXH3"/>
    <dbReference type="STRING" id="273057.SSO0800"/>
    <dbReference type="PaxDb" id="273057-SSO0800"/>
    <dbReference type="EnsemblBacteria" id="AAK41098">
        <property type="protein sequence ID" value="AAK41098"/>
        <property type="gene ID" value="SSO0800"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0800"/>
    <dbReference type="HOGENOM" id="CLU_2257492_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXH3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="103" mass="12000" checksum="63A711C7A20A2E8D" modified="2011-05-31" version="1">MFKFVIATTSRSNEGQRKAISTIFLPHFGQETEEYLWINIFNNHSVLLSLPFYPILNTTILHQIEFIVKLSWQFINVLNEVLQVLQLYNVGTQFLSNLNNPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXI3</accession>
    <name>Q7LXI3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acetylornithine deacetylase (ArgE-1)</fullName>
            <ecNumber evidence="1">3.5.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argE-1</name>
        <name evidence="1" type="ordered locus">SSO0790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.16" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99229">
        <property type="entry name" value="A99229"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923083.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXI3"/>
    <dbReference type="FunCoup" id="Q7LXI3">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0790"/>
    <dbReference type="PaxDb" id="273057-SSO0790"/>
    <dbReference type="EnsemblBacteria" id="AAK41088">
        <property type="protein sequence ID" value="AAK41088"/>
        <property type="gene ID" value="SSO0790"/>
    </dbReference>
    <dbReference type="GeneID" id="71027415"/>
    <dbReference type="KEGG" id="sso:SSO0790"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.796"/>
    <dbReference type="eggNOG" id="arCOG01107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1465183_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXI3"/>
    <dbReference type="PhylomeDB" id="Q7LXI3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008777">
        <property type="term" value="F:acetylornithine deacetylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006592">
        <property type="term" value="P:ornithine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF32">
        <property type="entry name" value="ARGE_DAPE-RELATED DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="184" mass="20168" checksum="544B27C57CDFA276" modified="2011-05-31" version="1">MVGDKAYGKGVSDMKGGVVSMYLALSETKRPVEISLVPDEESGGIGTKYLTEVRKIRAKYVIIGDPSFPNIYIGHLGIIRGVIRLIEKQAHASKTKEGENAFINGAKLALEIQKIYGVKEVSLNLGGYTINSSDNDGVVPGEFVFSFYRSTLQKNESLAIHGRLKITSLSVSTRWKNSLDQYPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXK2</accession>
    <name>Q7LXK2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0763</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90225">
        <property type="entry name" value="E90225"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991351.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXK2"/>
    <dbReference type="STRING" id="273057.SSO0763"/>
    <dbReference type="PaxDb" id="273057-SSO0763"/>
    <dbReference type="EnsemblBacteria" id="AAK41060">
        <property type="protein sequence ID" value="AAK41060"/>
        <property type="gene ID" value="SSO0763"/>
    </dbReference>
    <dbReference type="GeneID" id="1455027"/>
    <dbReference type="KEGG" id="sso:SSO0763"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.762"/>
    <dbReference type="eggNOG" id="arCOG05947">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1340783_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXK2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="204" mass="23332" checksum="B9FCE030E0AFD1D3" modified="2011-05-31" version="1">MKLKISWIELSQDLLPHSDLDSPEDLKLISNEILEAFEIGGHSEEIELDDKILTITSIFSSKLLQDIPKSIRIYEMGRWGKLLSGDVVTVIGETITYALLNQLFNISINDILPFRNVKFLGTISDLAINIEKYDTLRKFLNAKSGLLFVEAKATMTFRRSQIVNTISKSLVTIENLRYPDNYGLISYIIKYNNQLYDLMILIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXK5</accession>
    <name>Q7LXK5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF655 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90223">
        <property type="entry name" value="F90223"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991328.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXK5"/>
    <dbReference type="STRING" id="273057.SSO0750"/>
    <dbReference type="PaxDb" id="273057-SSO0750"/>
    <dbReference type="EnsemblBacteria" id="AAK41045">
        <property type="protein sequence ID" value="AAK41045"/>
        <property type="gene ID" value="SSO0750"/>
    </dbReference>
    <dbReference type="GeneID" id="1455012"/>
    <dbReference type="KEGG" id="sso:SSO0750"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.745"/>
    <dbReference type="eggNOG" id="arCOG04130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076814_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LXK5"/>
    <dbReference type="PhylomeDB" id="Q7LXK5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.280">
        <property type="entry name" value="AF1531-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007003">
        <property type="entry name" value="DUF655"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734:SF1">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04919">
        <property type="entry name" value="DUF655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160975">
        <property type="entry name" value="AF1531-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="24661" checksum="601B82E4FA76CB34" modified="2011-05-31" version="1">MQRKRIVRERVVYVLDYMREGNPLDKHKFHRDKPLIQAVGEDYFLLLELTPLSYNLDFLPEDKIELDSNSSVKVDAHISYEDLTSVSRDNLPKILYKIVFEKEKVFVEIFNKAEPLTLRLHALELLPNIGKKTLRIILEERKKKPFESFKDIESRIGVKDVASILVERIIKEMQGGEKYYLFVYPLIPDENKRLEQQFIYVGYIEKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXM7</accession>
    <name>Q7LXM7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Archaeal glutamate synthase [NADPH]</fullName>
            <ecNumber evidence="3">1.4.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">gltB</name>
        <name evidence="6" type="ordered locus">SSO0684</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2 L-glutamate + NADP(+) = L-glutamine + 2-oxoglutarate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="1.4.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.13" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90216">
        <property type="entry name" value="B90216"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXM7"/>
    <dbReference type="STRING" id="273057.SSO0684"/>
    <dbReference type="PaxDb" id="273057-SSO0684"/>
    <dbReference type="EnsemblBacteria" id="AAK40985">
        <property type="protein sequence ID" value="AAK40985"/>
        <property type="gene ID" value="SSO0684"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0684"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.684"/>
    <dbReference type="eggNOG" id="arCOG00619">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_398833_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXM7"/>
    <dbReference type="PhylomeDB" id="Q7LXM7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004355">
        <property type="term" value="F:glutamate synthase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024188">
        <property type="entry name" value="GltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043578">
        <property type="entry name" value="GltB_archl_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006982">
        <property type="entry name" value="Glu_synth_centr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43819">
        <property type="entry name" value="ARCHAEAL-TYPE GLUTAMATE SYNTHASE [NADPH]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43819:SF1">
        <property type="entry name" value="ARCHAEAL-TYPE GLUTAMATE SYNTHASE [NADPH]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04898">
        <property type="entry name" value="Glu_syn_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500061">
        <property type="entry name" value="GOGAT_lg2_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006429">
        <property type="entry name" value="GOGAT_lg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="2 3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate synthase" evidence="4">
        <location>
            <begin position="69"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamate synthase central-N" evidence="5">
        <location>
            <begin position="462"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR006429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="747" mass="81706" checksum="AFC36783C278381F" modified="2011-05-31" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXP7</accession>
    <name>Q7LXP7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">B3/B4 tRNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0658</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90213">
        <property type="entry name" value="D90213"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991198.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXP7"/>
    <dbReference type="SMR" id="Q7LXP7"/>
    <dbReference type="STRING" id="273057.SSO0658"/>
    <dbReference type="PaxDb" id="273057-SSO0658"/>
    <dbReference type="DNASU" id="1454930"/>
    <dbReference type="EnsemblBacteria" id="AAK40963">
        <property type="protein sequence ID" value="AAK40963"/>
        <property type="gene ID" value="SSO0658"/>
    </dbReference>
    <dbReference type="GeneID" id="1454930"/>
    <dbReference type="KEGG" id="sso:SSO0658"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.660"/>
    <dbReference type="eggNOG" id="arCOG04250">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076869_2_0_2"/>
    <dbReference type="InParanoid" id="Q7LXP7"/>
    <dbReference type="PhylomeDB" id="Q7LXP7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39209">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39209:SF2">
        <property type="entry name" value="CYTOPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56037">
        <property type="entry name" value="PheT/TilS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B3/B4 tRNA-binding" evidence="1">
        <location>
            <begin position="61"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="224" mass="25301" checksum="8F204023C7D83B5D" modified="2011-05-31" version="1">MKRLSISEDVRRLGVFVAMTEVSNVDAKRPLDTLDDIIRNLEEKYASQDVESLRNDPVVRAYRDFYWRIGIDPTKTRPSGEALRRRLARGNKLPRINIVVDIGNIVSAETLVPIGLYDRDKIVGDLHLVMSKGNEEFLGLGKKKEKLDKGIPILVDDEGKVLHIYPHRDSVLTSITLNVKNVVIIGAGVPNIDENLVKYAVDRVADLLEKVCNGKREYNTMVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXT8</accession>
    <name>Q7LXT8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Acetolactate synthase</fullName>
            <ecNumber evidence="13">2.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ilvB-2</name>
        <name evidence="17" type="ordered locus">SSO0579</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 pyruvate + H(+) = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="13">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="13"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90204">
        <property type="entry name" value="E90204"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991094.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXT8"/>
    <dbReference type="FunCoup" id="Q7LXT8">
        <property type="interactions" value="393"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0579"/>
    <dbReference type="PaxDb" id="273057-SSO0579"/>
    <dbReference type="EnsemblBacteria" id="AAK40892">
        <property type="protein sequence ID" value="AAK40892"/>
        <property type="gene ID" value="SSO0579"/>
    </dbReference>
    <dbReference type="GeneID" id="1454859"/>
    <dbReference type="KEGG" id="sso:SSO0579"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.586"/>
    <dbReference type="eggNOG" id="arCOG01998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_1_2_2"/>
    <dbReference type="InParanoid" id="Q7LXT8"/>
    <dbReference type="PhylomeDB" id="Q7LXT8"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02015">
        <property type="entry name" value="TPP_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000007">
        <property type="entry name" value="Acetolactate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012846">
        <property type="entry name" value="Acetolactate_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039368">
        <property type="entry name" value="AHAS_TPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00118">
        <property type="entry name" value="acolac_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005039">
        <property type="entry name" value="PRK06456.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="7 13 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="16">
        <location>
            <begin position="3"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="14">
        <location>
            <begin position="197"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="15">
        <location>
            <begin position="394"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AAK40892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="572" mass="63212" checksum="CE201C1C9B97598C" modified="2011-05-31" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-06-18" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXU1</accession>
    <name>Q7LXU1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90204">
        <property type="entry name" value="A90204"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXU1"/>
    <dbReference type="STRING" id="273057.SSO0572"/>
    <dbReference type="PaxDb" id="273057-SSO0572"/>
    <dbReference type="EnsemblBacteria" id="AAK40888">
        <property type="protein sequence ID" value="AAK40888"/>
        <property type="gene ID" value="SSO0572"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0572"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.582"/>
    <dbReference type="eggNOG" id="arCOG04116">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023387_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXU1"/>
    <dbReference type="PhylomeDB" id="Q7LXU1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052041">
        <property type="entry name" value="Nucleic_acid_metab_PIN/TRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010335">
        <property type="entry name" value="PRK13764.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11603">
        <property type="entry name" value="AAA FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11603:SF147">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="7"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="510" mass="57838" checksum="8115CDA271B20AC0" modified="2011-05-31" version="1">MGIRILNDLMLDKSALLFGVSKYLEKGIITGNILIHKSLLAELEKESNDGLVSAEIALDEVKKLKDITERILVNFEIVGENSKKGEANELSREYCLEKGCIMVTADETQKKICDVMGIQYSFLQPLEQSLSFESFFDDETMSLHIKEDTVPRAKKGKPGNWKFVNLSNKPMLSVDVKRIANEIINGVRFIKGSFIEIERKGSLIIQLGNYRVVITRPPLSDGWEITITRPVVRKRLEDYGLDERLIKRLEERAEGIIIAGAPGMGKTTFAQALSEYYMRLGKIVKTIESPRDMHLPPEITQYSKNYAEIGELHDILLLSRPDYTVYDEMRNDEDFKLYIDLRLAGIGMVGVVHATSPIDAIHRFVNRVDIGTIPNILDTIIFINSGNVSKVYTLEITVKVPAGLKEADLARPVVEIKDLVTGNTEYEIYVFGEQTMIVPVNRGITLNNMEFKISKIVNNIIPKATVKYEDGEYVIVIPKEEIGKYNRKLVQRLKRLEKKNNIKIKIKLSD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q7SI95</accession>
    <accession>Q980W7</accession>
    <name>LYSX_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Alpha-aminoadipate--LysW ligase LysX</fullName>
            <shortName>AAA--LysW ligase LysX</shortName>
            <ecNumber>6.3.2.43</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">lysX</name>
        <name type="ordered locus">SSO0159</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="29537" last="29549">
            <title>The Sulfolobus solfataricus Lrp-like protein LysM regulates lysine biosynthesis in response to lysine availability.</title>
            <authorList>
                <person name="Brinkman A.B."/>
                <person name="Bell S.D."/>
                <person name="Lebbink R.J."/>
                <person name="de Vos W.M."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="12042311"/>
            <dbReference type="DOI" id="10.1074/jbc.M203528200"/>
        </citation>
        <scope>FUNCTION IN LYSINE BIOSYNTHESIS</scope>
        <scope>INDUCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the ATP-dependent formation of a covalent bond between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of the C-terminal glutamate residue in LysW.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[amino-group carrier protein]-C-terminal-L-glutamate + L-2-aminoadipate + ATP = [amino-group carrier protein]-C-terminal-N-(1,4-dicarboxybutan-1-yl)-L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41940"/>
            <dbReference type="Rhea" id="RHEA-COMP:9693"/>
            <dbReference type="Rhea" id="RHEA-COMP:9694"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="ChEBI" id="CHEBI:78503"/>
            <dbReference type="ChEBI" id="CHEBI:78525"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.43"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="induction">
        <text evidence="4">Activated by LysM and repressed by lysine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RimK family. LysX subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40505" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.43"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40505.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BK000545">
        <property type="protein sequence ID" value="DAA00053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90156">
        <property type="entry name" value="B90156"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7SI95"/>
    <dbReference type="SMR" id="Q7SI95"/>
    <dbReference type="FunCoup" id="Q7SI95">
        <property type="interactions" value="139"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0159"/>
    <dbReference type="PaxDb" id="273057-SSO0159"/>
    <dbReference type="EnsemblBacteria" id="AAK40505">
        <property type="protein sequence ID" value="AAK40505"/>
        <property type="gene ID" value="SSO0159"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0159"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.156"/>
    <dbReference type="eggNOG" id="arCOG01589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054353_2_1_2"/>
    <dbReference type="InParanoid" id="Q7SI95"/>
    <dbReference type="PhylomeDB" id="Q7SI95"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00035"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043774">
        <property type="term" value="F:coenzyme F420-2 alpha-glutamyl ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000025">
        <property type="entry name" value="Alpha-aminoadipate--LysW ligase LysX protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000058">
        <property type="entry name" value="Alpha-aminoadipate--LysW ligase LysX protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000042">
        <property type="entry name" value="RimK-related lysine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054562">
        <property type="entry name" value="LysX/ArgX_preATP_grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011870">
        <property type="entry name" value="LysX_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="Rp_bS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02144">
        <property type="entry name" value="LysX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF2">
        <property type="entry name" value="COENZYME GAMMA-F420-2:ALPHA-L-GLUTAMATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22626">
        <property type="entry name" value="LysX_preATP_grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000205500" description="Alpha-aminoadipate--LysW ligase LysX">
        <location>
            <begin position="1"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="3">
        <location>
            <begin position="85"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="N-[TS] motif that is essential for LysX substrate specificity">
        <location>
            <begin position="252"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="125"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q4JAP9"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="12042311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000305" key="6">
        <source>
            <dbReference type="PubMed" id="12042311"/>
        </source>
    </evidence>
    <sequence length="275" mass="32028" checksum="038E3DD3B4FC8800" modified="2003-12-15" version="1">MPRWEEKNLIEEGKKLGYQVATIYSKDFVLFSNDFTIDNDTDLFIQRNVSHNRALITSFLVEQVGYPVINDHTTLIRCENKIFTTYILARHNIPTPKTFIAFDKTNAIEYSKKLGYPVVIKPVEGSWGRMVAKADNLDVLYSYLEYQEFSTQKYKDIYYIQEFVNKPNRDIRIFVIGDETPVGIYRVNENNWRTNTALGAKAYPLKIDEELRELALKVKDIIGGFFLGIDIFEDKDRGYLVDEVNGVPEYKNTVRVNNFNVSKFLLEKAAEWVKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2013-05-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TX9</accession>
    <name>CAS4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated exonuclease Cas4</fullName>
            <ecNumber evidence="2 3">3.1.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cas4</name>
        <name type="ordered locus">SSO0001</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="PLoS ONE" volume="7" first="E47232" last="E47232">
            <title>The CRISPR associated protein Cas4 is a 5' to 3' DNA exonuclease with an iron-sulfur cluster.</title>
            <authorList>
                <person name="Zhang J."/>
                <person name="Kasciukovic T."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="23056615"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0047232"/>
        </citation>
        <scope>FUNCTION AS AN EXONUCLEASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF ASP-99</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2013" name="J. Am. Chem. Soc." volume="135" first="17476" last="17487">
            <title>Toroidal structure and DNA cleavage by the CRISPR-associated [4Fe-4S] cluster containing Cas4 nuclease SSO0001 from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Lemak S."/>
                <person name="Beloglazova N."/>
                <person name="Nocek B."/>
                <person name="Skarina T."/>
                <person name="Flick R."/>
                <person name="Brown G."/>
                <person name="Popovic A."/>
                <person name="Joachimiak A."/>
                <person name="Savchenko A."/>
                <person name="Yakunin A.F."/>
            </authorList>
            <dbReference type="PubMed" id="24171432"/>
            <dbReference type="DOI" id="10.1021/ja408729b"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR (4FE-4S) AND MANGANESE</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>DNA-BINDING</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF CYS-32; ASP-99; LYS-115; TYR-148; CYS-188; CYS-191 AND CYS-197</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 3">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). This protein is a 5' to 3' partially processive exonuclease that cleaves off single mononucleotides. Has a marked preference for ssDNA, although in vitro it also acts on dsDNA and ssRNA. Has low endonuclease activity with circular ssDNA. Binds ssDNA and can unwind dsDNA; unwinding does not require ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates.</text>
            <dbReference type="EC" id="3.1.12.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
        <text evidence="2 3">Mg(2+) or Mn(2+) required for ssDNA cleavage activity. Can also utilise Cu(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3 4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2 3 4">Binds 1 [4Fe-4S] cluster per subunit (PubMed:24171432). It may be important for protein stability, since mutation of the Cys that bind the cofactor leads to a colorless, insoluble protein.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="3">Optimum pH is 9-10.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="3">Optimum temperature is 65-70 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="2 3">Homodecamer, formed by the assembly of dimers into a decameric toroid, with the active sites oriented towards the central tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CRISPR-associated exonuclease Cas4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.12.1" evidence="2 3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90139">
        <property type="entry name" value="A90139"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989652.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="4IC1">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.35 A"/>
        <property type="chains" value="A/B/C/D/F/G/H/I/J/K=1-202"/>
    </dbReference>
    <dbReference type="PDBsum" id="4IC1"/>
    <dbReference type="AlphaFoldDB" id="Q97TX9"/>
    <dbReference type="SMR" id="Q97TX9"/>
    <dbReference type="STRING" id="273057.SSO0001"/>
    <dbReference type="PaxDb" id="273057-SSO0001"/>
    <dbReference type="EnsemblBacteria" id="AAK43340">
        <property type="protein sequence ID" value="AAK43340"/>
        <property type="gene ID" value="SSO0001"/>
    </dbReference>
    <dbReference type="GeneID" id="1455258"/>
    <dbReference type="GeneID" id="44128964"/>
    <dbReference type="KEGG" id="sso:SSO0001"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2"/>
    <dbReference type="eggNOG" id="arCOG00790">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106570_0_0_2"/>
    <dbReference type="InParanoid" id="Q97TX9"/>
    <dbReference type="PhylomeDB" id="Q97TX9"/>
    <dbReference type="BRENDA" id="3.1.12.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97TX9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045145">
        <property type="term" value="F:single-stranded DNA 5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051827">
        <property type="entry name" value="Cas4_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013343">
        <property type="entry name" value="CRISPR-assoc_prot_Cas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00372">
        <property type="entry name" value="cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531">
        <property type="entry name" value="CRISPR-ASSOCIATED EXONUCLEASE CAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531:SF2">
        <property type="entry name" value="CRISPR-ASSOCIATED EXONUCLEASE CAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000422224" description="CRISPR-associated exonuclease Cas4">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Abolishes iron-sulfur (4Fe-4S) binding and exonuclease activity; when associated with A-188; A-191 and A-197." evidence="3">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abolishes nuclease activity." evidence="2 3">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Impairs exonuclease activity." evidence="3">
        <original>E</original>
        <variation>A</variation>
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abolishes exonuclease activity." evidence="3">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Nearly abolishes exonuclease activity." evidence="3">
        <original>Y</original>
        <variation>A</variation>
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abolishes iron-sulfur (4Fe-4S) binding and exonuclease activity; when associated with A-32; A-191 and A-197." evidence="3">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abolishes iron-sulfur (4Fe-4S) binding and exonuclease activity; when associated with A-32; A-188 and A-197." evidence="3">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abolishes iron-sulfur (4Fe-4S) binding and exonuclease activity; when associated with A-32; A-188 and A-191." evidence="3">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="23"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="26"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="33"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="43"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="47"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="105"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="110"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="142"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="153"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="165"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="180"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="188"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="194"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="23056615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="24171432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="4IC1"/>
        </source>
    </evidence>
    <sequence length="202" mass="23239" checksum="C1E8868ACC093382" modified="2001-10-01" version="1">MITEFLLKKKLEEHLSHVKEENTIYVTDLVRCPRRVRYESEYKELAISQVYAPSAILGDILHLGLESVLKGNFNAETEVETLREINVGGKVYKIKGRADAIIRNDNGKSIVIEIKTSRSDKGLPLIHHKMQLQIYLWLFSAEKGILVYITPDRIAEYEINEPLDEATIVRLAEDTIMLQNSPRFNWECKYCIFSVICPAKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TZ0</accession>
    <name>Q97TZ0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dolichol-phosphate mannosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.83</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO3241</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.83" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90509">
        <property type="entry name" value="G90509"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TZ0"/>
    <dbReference type="SMR" id="Q97TZ0"/>
    <dbReference type="FunCoup" id="Q97TZ0">
        <property type="interactions" value="222"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3241"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3241"/>
    <dbReference type="EnsemblBacteria" id="AAK43334">
        <property type="protein sequence ID" value="AAK43334"/>
        <property type="gene ID" value="SSO3241"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3241"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3341"/>
    <dbReference type="eggNOG" id="arCOG00895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_13_0_2"/>
    <dbReference type="InParanoid" id="Q97TZ0"/>
    <dbReference type="PhylomeDB" id="Q97TZ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06442">
        <property type="entry name" value="DPM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000122">
        <property type="entry name" value="Dolichol-phosphate mannosyltransferase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398:SF1">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="4">
        <location>
            <begin position="27"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="255" mass="29285" checksum="D294CB6631AD2179" modified="2001-10-01" version="1">MKRRESSPLISTILGIRYGRCVMGNFSIVIPTYNERDNIVKLVEEINRIVPYNSRILIVDDNSPDGTALILQELNIHNLTVLIRHNERGLGSALRYGISKAIELESDFIVTMDADFSHNPKYLPEMMKIALNENCDLVIGSRYVIGGGIENWSLSRRIISKGANYLFKLVSHSPIMDNTSNYRIYSRRAALLALECDTTNGYEFQICSIFKIIRNNLKIKEYPIIFVDRKTGKSKLNLKEILNWFLYIIRLSLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TZ5</accession>
    <name>Q97TZ5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Probable cobalamin biosynthesis protein CobD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cbiB</name>
        <name evidence="11" type="synonym">cobD</name>
        <name evidence="12" type="ordered locus">SSO3235</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the CobD/CbiB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90509">
        <property type="entry name" value="B90509"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989674.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TZ5"/>
    <dbReference type="FunCoup" id="Q97TZ5">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3235"/>
    <dbReference type="PaxDb" id="273057-SSO3235"/>
    <dbReference type="EnsemblBacteria" id="AAK43329">
        <property type="protein sequence ID" value="AAK43329"/>
        <property type="gene ID" value="SSO3235"/>
    </dbReference>
    <dbReference type="GeneID" id="1453248"/>
    <dbReference type="KEGG" id="sso:SSO3235"/>
    <dbReference type="eggNOG" id="arCOG04274">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054212_0_2_2"/>
    <dbReference type="InParanoid" id="Q97TZ5"/>
    <dbReference type="PhylomeDB" id="Q97TZ5"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00024">
        <property type="entry name" value="CobD_CbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004485">
        <property type="entry name" value="Cobalamin_biosynth_CobD/CbiB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00380">
        <property type="entry name" value="cobal_cbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002281">
        <property type="entry name" value="PRK01209.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308">
        <property type="entry name" value="COBALAMIN BIOSYNTHESIS PROTEIN CBIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308:SF1">
        <property type="entry name" value="COBALAMIN BIOSYNTHESIS PROTEIN CBIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03186">
        <property type="entry name" value="CobD_Cbib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK43329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="305" mass="34567" checksum="118CD146A9CD3599" modified="2001-10-01" version="1">MLFILALALIWDLTISEPPIYIHPVVWTGKISEILIKPYKGYFYGVILWILSVLPPLLIFVYLPIVAEIPIYLKIIILTFSLKTTFSIKMLYDIVTKGSRLDDQARYYAQQIVRRDLSKSEKGHIASALIESLFESTVDGITSPIFWFLIFGLIGAMLQRLANTMDSMVGYKTPELRKEGWFSAKIDTILNYIPARLTGMIMLIAGLILGLDVKQGWKALREAKMESVNAKYPIAIAAGLLNVSLEKVGHYKVGFGNLPCEDDIEKALKLFKLSLILYFLVISTIYYYLYGISLLTYPYGLIELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TZ7</accession>
    <name>Q97TZ7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Probable cobyric acid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cobQ</name>
        <name evidence="10" type="ordered locus">SSO3233</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CobB/CobQ family. CobQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90508">
        <property type="entry name" value="H90508"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989676.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TZ7"/>
    <dbReference type="FunCoup" id="Q97TZ7">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3233"/>
    <dbReference type="PaxDb" id="273057-SSO3233"/>
    <dbReference type="DNASU" id="1453246"/>
    <dbReference type="EnsemblBacteria" id="AAK43327">
        <property type="protein sequence ID" value="AAK43327"/>
        <property type="gene ID" value="SSO3233"/>
    </dbReference>
    <dbReference type="GeneID" id="1453246"/>
    <dbReference type="KEGG" id="sso:SSO3233"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3335"/>
    <dbReference type="eggNOG" id="arCOG00105">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019250_2_2_2"/>
    <dbReference type="InParanoid" id="Q97TZ7"/>
    <dbReference type="PhylomeDB" id="Q97TZ7"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01750">
        <property type="entry name" value="GATase1_CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00028">
        <property type="entry name" value="CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033949">
        <property type="entry name" value="CobQ_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004459">
        <property type="entry name" value="CobQ_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00313">
        <property type="entry name" value="cobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001989">
        <property type="entry name" value="PRK00784.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343:SF1">
        <property type="entry name" value="COBYRIC ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343">
        <property type="entry name" value="DETHIOBIOTIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="8">
        <location>
            <begin position="3"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="CobB/CobQ-like glutamine amidotransferase" evidence="9">
        <location>
            <begin position="236"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK43327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="465" mass="52328" checksum="B2B54A9665AD4698" modified="2001-10-01" version="1">MSIIVASTMSDSGKSFITTGLVRILNAKPLKIQNMSLNSISTWDGGEIAFIQAYQAWGAGLRPERYMNPILLKIMGKGLEVIFMGSSLGIMSGNEYYNLATEKLWKKVSQFLSNDVVIEAAGGIGEPNFIDKDITAIKVMKEKRIPAILVLDIDRGGAFASAYGTYMMLPQSVRENLKGFIINKFRGDEKFLNDAIKWLEEKTGMRYLGYLPFFSEPPIMPEDSMNVYNFGDGNVEVAIIAYPYMSNFNEFYALVKSNARVSFVRKPKETAKADLVILPGSRNTLESLKWLVENGFVEQLRQRSMNILGICGGFQILGKKIVDPYGIEAGYPTEYEGLGIFDINVHYSKDKVVSMTTGISEYGEMNGYEIRRGVIKYMGEKPLLKITLRNGEKVSVEDGAKKDNIIGISVHGTLFSDGMKKLLKEFNINIHSQSMEEEIKQQAIRVEQFLRKYLHIDEIVEIYKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2013-06-26" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U27</accession>
    <name>GAD_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">D-gluconate/D-galactonate dehydratase</fullName>
            <shortName evidence="4">GAD</shortName>
            <shortName evidence="4">GNAD</shortName>
            <ecNumber evidence="2 3">4.2.1.140</ecNumber>
            <ecNumber evidence="2 3">4.2.1.39</ecNumber>
            <ecNumber evidence="2 3">4.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gad</name>
        <name evidence="5" type="synonym">gnaD</name>
        <name type="ordered locus">SSO3198</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="FEBS Lett." volume="576" first="133" last="136">
            <title>Gluconate dehydratase from the promiscuous Entner-Doudoroff pathway in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Lamble H.J."/>
                <person name="Milburn C.C."/>
                <person name="Taylor G.L."/>
                <person name="Hough D.W."/>
                <person name="Danson M.J."/>
            </authorList>
            <dbReference type="PubMed" id="15474024"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2004.08.074"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-5</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>COFACTOR</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2005" name="Biochem. J." volume="387" first="271" last="280">
            <title>Identification and characterization of Sulfolobus solfataricus D-gluconate dehydratase: a key enzyme in the non-phosphorylated Entner-Doudoroff pathway.</title>
            <authorList>
                <person name="Kim S."/>
                <person name="Lee S.B."/>
            </authorList>
            <dbReference type="PubMed" id="15509194"/>
            <dbReference type="DOI" id="10.1042/BJ20041053"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-10</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>COFACTOR</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>SUBUNIT</scope>
        <scope>NOMENCLATURE</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Involved in the degradation of glucose and galactose via the branched variant of the Entner-Doudoroff pathway. Catalyzes the dehydration of gluconate to produce 2-keto-3-deoxygluconate (KDG). It is also able to catalyze the dehydration of galactonate to produce 2-keto-3-deoxygalactonate (KDGal).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18391"/>
            <dbReference type="ChEBI" id="CHEBI:57990"/>
            <dbReference type="EC" id="4.2.1.140"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18391"/>
            <dbReference type="ChEBI" id="CHEBI:57990"/>
            <dbReference type="EC" id="4.2.1.39"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O</text>
            <dbReference type="Rhea" id="RHEA:18649"/>
            <dbReference type="ChEBI" id="CHEBI:12931"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57989"/>
            <dbReference type="EC" id="4.2.1.140"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O</text>
            <dbReference type="Rhea" id="RHEA:18649"/>
            <dbReference type="ChEBI" id="CHEBI:12931"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57989"/>
            <dbReference type="EC" id="4.2.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="2 3">Binds 1 Mg(2+) ion per subunit. Can also use divalent metal ions such as Co(2+), Mn(2+) and Ni(2+).</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">0.45 mM for gluconate (at 78 degrees Celsius and pH 7)</KM>
            <KM evidence="2">0.81 mM for galactonate (at 70 degrees Celsius and pH 6)</KM>
            <KM evidence="2">1.57 mM for gluconate (at 70 degrees Celsius and pH 6)</KM>
            <Vmax evidence="3">0.15 umol/min/mg enzyme with gluconate as substrate (at 78 degrees Celsius and pH 7)</Vmax>
            <text evidence="2 3">kcat is 10.4 sec(-1) for gluconate and 1.08 sec(-1) for galactonate (at 70 degrees Celsius and pH 6). kcat is 12.6 sec(-1) for gluconate (at 78 degrees Celsius and pH 7).</text>
        </kinetics>
        <phDependence>
            <text evidence="2 3">Optimum pH is between 6.5 and 7.5.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2 3">Optimum temperature is between 80 and 100 degrees Celsius. At 80 degrees Celsius, the dehydratase is stable for over 2 hours, but at 90 degrees Celsius its activity is decreased to less than 50% in 2 hours and its half-life diminishes to 120 minutes. At 100 degrees Celsius, the enzyme has a half-life of less than 40 minutes. The activity is almost undetectable below 60 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate acid metabolism; D-gluconate degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3">Monomer or homooctamer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="5">D-gluconate dehydratase activity is lost after incubation with phosphatase, whereas no such decrease in activity is observed when the enzymes are incubated in the same reaction mixtures without phosphatase. Analysis of the amino acid sequence of D-gluconate dehydratase indicate the presence of several putative phosphorylation sites on serine, threonine and tyrosine residues.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mandelate racemase/muconate lactonizing enzyme family. GaD subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.140" evidence="2 3"/>
    <dbReference type="EC" id="4.2.1.39" evidence="2 3"/>
    <dbReference type="EC" id="4.2.1.6" evidence="2 3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90504">
        <property type="entry name" value="H90504"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991686.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U27"/>
    <dbReference type="SMR" id="Q97U27"/>
    <dbReference type="FunCoup" id="Q97U27">
        <property type="interactions" value="177"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3198"/>
    <dbReference type="PaxDb" id="273057-SSO3198"/>
    <dbReference type="EnsemblBacteria" id="AAK43295">
        <property type="protein sequence ID" value="AAK43295"/>
        <property type="gene ID" value="SSO3198"/>
    </dbReference>
    <dbReference type="GeneID" id="1453214"/>
    <dbReference type="KEGG" id="sso:SSO3198"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3301"/>
    <dbReference type="eggNOG" id="arCOG01168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_3_2_2"/>
    <dbReference type="InParanoid" id="Q97U27"/>
    <dbReference type="PhylomeDB" id="Q97U27"/>
    <dbReference type="BRENDA" id="4.2.1.140">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q97U27"/>
    <dbReference type="UniPathway" id="UPA00792"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008869">
        <property type="term" value="F:galactonate dehydratase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047929">
        <property type="term" value="F:gluconate dehydratase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03316">
        <property type="entry name" value="MR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000005">
        <property type="entry name" value="Putative L-rhamnonate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034593">
        <property type="entry name" value="DgoD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034599">
        <property type="entry name" value="Gluconate_dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080:SF2">
        <property type="entry name" value="D-GALACTONATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080">
        <property type="entry name" value="D-GALACTONATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00008">
        <property type="entry name" value="gluconate_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00179">
        <property type="entry name" value="mandelate_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000422650" description="D-gluconate/D-galactonate dehydratase">
        <location>
            <begin position="1"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Increases basicity of active site His" evidence="1">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15474024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="15509194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="15474024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="15509194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="395" mass="44729" checksum="BA53EFD93F90A44D" modified="2001-10-01" version="1">MRIREIEPIVLTSKEKGSATWASIMIVTRVITENGEVGYGEAVPTLRVISVYNAIKQVSKAYIGKEVEEVEKNYHEWYKQDFYLARSFESATAVSAIDIASWDIIGKELGAPIHKLLGGKTRDRVPVYANGWYQDCVTPEEFAEKAKDVVKMGYKALKFDPFGPYYDWIDERGLREAEERVKAVREAVGDNVDILIEHHGRFNANSAIMIAKRLEKYNPGFMEEPVHHEDVIGLRKYKASTHLRVALGERLISEKETAFYVEEGLVNILQPDLTNIGGVTVGRSVIKIAEANDVEVAFHNAFGSIQNAVEIQLSAVTQNLYLLENFYDWFPQWKRDLVYNETPVEGGHVKVPYKPGLGVSINEKIIEQLRAEPIPLDVIEEPVWVVKGTWKNYGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U60</accession>
    <name>Q97U60_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycolate oxidase glcD subunit. (GlcD)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">glcD</name>
        <name evidence="6" type="ordered locus">SSO3163</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="G90500"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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        <property type="interactions" value="219"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3163"/>
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    <dbReference type="DNASU" id="1453181"/>
    <dbReference type="EnsemblBacteria" id="AAK43262">
        <property type="protein sequence ID" value="AAK43262"/>
        <property type="gene ID" value="SSO3163"/>
    </dbReference>
    <dbReference type="GeneID" id="1453181"/>
    <dbReference type="KEGG" id="sso:SSO3163"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3267"/>
    <dbReference type="eggNOG" id="arCOG00337">
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    <dbReference type="HOGENOM" id="CLU_017779_9_2_2"/>
    <dbReference type="InParanoid" id="Q97U60"/>
    <dbReference type="PhylomeDB" id="Q97U60"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019154">
        <property type="term" value="F:glycolate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046296">
        <property type="term" value="P:glycolate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051914">
        <property type="entry name" value="FAD-linked_OxidoTrans_Type4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42934">
        <property type="entry name" value="GLYCOLATE OXIDASE SUBUNIT GLCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42934:SF1">
        <property type="entry name" value="GLYCOLATE OXIDASE SUBUNIT GLCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="5">
        <location>
            <begin position="31"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="467" mass="51325" checksum="DBD2797CB0216C73" modified="2001-10-01" version="1">MISKLAEIVGEEWVISGDEAKLYSYPAFIPLKIEPPVVVLPGSEEEVISLVNFLNENNIKYIVRGSGTSLSGATTPTQGEVVISLTRLNRIYSLDNFEITVGPGLANFLINKTLGNSNLFYAPDPSSYVVSSIGGNISHDSGGMHTPKYGTTFDSIIRLKVLLSNGHVEEIGSSNFFDPTSIFVGAEGTLGVILRATLRLFPKPEVSKTILASFNSIDDAGKAVVSVYKAGVTPAAMEFLDKNSIIAIENTQYKANYPMAEAILLIELDGYSVNVEEEEVRVVSAIRNNNGEVFIPKDEEEKNRWWWGRKGAFPSFAYYSPAYLTLDANVPRSTLPTMLRFTYEISKKYGLPVANSFHAGDGTLHPLIGFDPKNADETIKAIKAAEEITKLAIKVGGVPSGEHGIGIEKIKFMKLYYSEDDLTLMRRLKATFDPKRLLNPCKLIPPAKNEICNTVNRIHTYLLEGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U98</accession>
    <name>Q97U98_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Metabolite transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO3121</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90495">
        <property type="entry name" value="H90495"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924139.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U98"/>
    <dbReference type="FunCoup" id="Q97U98">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3121"/>
    <dbReference type="EnsemblBacteria" id="AAK43223">
        <property type="protein sequence ID" value="AAK43223"/>
        <property type="gene ID" value="SSO3121"/>
    </dbReference>
    <dbReference type="GeneID" id="1453142"/>
    <dbReference type="KEGG" id="sso:SSO3121"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3228"/>
    <dbReference type="eggNOG" id="arCOG02693">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_5_2"/>
    <dbReference type="InParanoid" id="Q97U98"/>
    <dbReference type="PhylomeDB" id="Q97U98"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045:SF1">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="153"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="403"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="5">
        <location>
            <begin position="14"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="433" mass="46968" checksum="1FFED436C2840329" modified="2001-10-01" version="1">MGGREISTGQVIKVAVGTSLGTTIEWYDFFLYGYAASLIFPKLFFPPSYNPLTALLVSLSTYAVGFVARPVGAMIFGHLGDKLGRRTGLLWDLILMGIGTGIIGFLPGYSQIGFLALTIATISRILQGIGVGGEWGGATTWLTEYAAKSRWRAFWGSWVQQGVPIGLLWASAILGLFATFPSSSPLALLNYGWRIAFWIGAIAAVIGIVIRLALLESPLFERVIEKGEVSKIPSIEVWRKYWSKILLLAASWAAQNAGFYLYSVYSVTFLTGIGFPKSLGLLSVTVSALIGIFITVLFGILSDKIGRRLGMMIAFGFGLAFSFPYVLLLFSKSFSIILLAQTLMVVAVLGSYAIIPAFFAENFPTKYRYSGTGLSYHMAAPFAGGLAPIIVADLVGEKYLVNWWAFGAVFAAYFIISLIALAVLKETKGKEMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UB0</accession>
    <name>Q97UB0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec-independent protein translocase protein TatC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tatC-like</name>
        <name evidence="5" type="synonym">tatC</name>
        <name evidence="6" type="ordered locus">SSO3108</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Forms a complex with TatA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TatC family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90494">
        <property type="entry name" value="D90494"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990930.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UB0"/>
    <dbReference type="FunCoup" id="Q97UB0">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3108"/>
    <dbReference type="PaxDb" id="273057-SSO3108"/>
    <dbReference type="EnsemblBacteria" id="AAK43211">
        <property type="protein sequence ID" value="AAK43211"/>
        <property type="gene ID" value="SSO3108"/>
    </dbReference>
    <dbReference type="GeneID" id="1453130"/>
    <dbReference type="GeneID" id="44128829"/>
    <dbReference type="KEGG" id="sso:SSO3108"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3215"/>
    <dbReference type="eggNOG" id="arCOG01919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031942_3_2_2"/>
    <dbReference type="InParanoid" id="Q97UB0"/>
    <dbReference type="PhylomeDB" id="Q97UB0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009977">
        <property type="term" value="F:proton motive force dependent protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002033">
        <property type="entry name" value="TatC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371:SF0">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01840">
        <property type="entry name" value="TATCFAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="289" mass="33079" checksum="F1524924184FD3CF" modified="2001-10-01" version="1">MEEKAKERGKEEKSFFEEKPLLDHLRELAIRLRRIIAALAISFFFYFMTGYEWINIDPLQIPFLGISISKIPIPYPSFYDSIAVQVTKFFIYHELPANTKIIIINLFDPIYASLYVSLYLALLTSLPLIIRETWAFLAPGLYKHEKKIFKLTMIPAFLLFFSGSLFAFLILIPLMFDMISFYTESLGPAVEPTISLSSFISTTFLLMGSLGLAFEMPLIMIGLTHLRIVKASTWRNYWRWGVLVSFIIAWIISPGTTGGVMETIIGLSLSSLYFLGMGISFLVERKRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UE2</accession>
    <name>Q97UE2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SpoVT-AbrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO11914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90490">
        <property type="entry name" value="D90490"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989091.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UE2"/>
    <dbReference type="SMR" id="Q97UE2"/>
    <dbReference type="STRING" id="273057.SSO11914"/>
    <dbReference type="PaxDb" id="273057-SSO11914"/>
    <dbReference type="EnsemblBacteria" id="AAK43179">
        <property type="protein sequence ID" value="AAK43179"/>
        <property type="gene ID" value="SSO11914"/>
    </dbReference>
    <dbReference type="GeneID" id="15298671"/>
    <dbReference type="KEGG" id="sso:SSO11914"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3181"/>
    <dbReference type="eggNOG" id="arCOG00815">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158484_9_3_2"/>
    <dbReference type="InParanoid" id="Q97UE2"/>
    <dbReference type="PhylomeDB" id="Q97UE2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052975">
        <property type="entry name" value="Repressor-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860:SF6">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="73" mass="8267" checksum="94F9E02572851E3A" modified="2001-10-01" version="1">MERVKVTRNYQITIPASARSKINIKEGDILEVYLNGDEIVLRKVKTERPRIRLGSKLSLGDIEEAIERGEGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UH4</accession>
    <name>Q97UH4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter, ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO3045</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90486">
        <property type="entry name" value="B90486"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924108.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UH4"/>
    <dbReference type="FunCoup" id="Q97UH4">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3045"/>
    <dbReference type="PaxDb" id="273057-SSO3045"/>
    <dbReference type="EnsemblBacteria" id="AAK43145">
        <property type="protein sequence ID" value="AAK43145"/>
        <property type="gene ID" value="SSO3045"/>
    </dbReference>
    <dbReference type="GeneID" id="1453064"/>
    <dbReference type="KEGG" id="sso:SSO3045"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3142"/>
    <dbReference type="eggNOG" id="arCOG00204">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="InParanoid" id="Q97UH4"/>
    <dbReference type="PhylomeDB" id="Q97UH4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43230">
        <property type="entry name" value="ABC-TYPE DIPEPTIDE/OLIGOPEPTIDE TRANSPORT SYSTEM, ATPASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43230:SF3">
        <property type="entry name" value="ABC-TYPE DIPEPTIDE_OLIGOPEPTIDE TRANSPORT SYSTEM, ATPASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="267" mass="30451" checksum="EAE5F900B343B6FB" modified="2001-10-01" version="1">MSDKLIELIHVYKDFIVRKGVFGKEHRPGIWDVNMDIRKNEIVGMVGGSGGGKTVIAWMIVGLLKPTKGSIIYTPMGLDVTKANKKQLKQYRADVQLVFQDPYSSLDPAHTVKWHIERPLKIYKYRGDIEERIKELLREVALTPPEDFLNKYPFQLSGGQRQRVYLARVLALNPKVLIADEPVSNVDASIRASLLDLFKRLRDEKGISIMYITHDIATIGYVADRVYVVKNGRIVEEGDVGTIISHPKHEYTRLLIEAVPDPYKRIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UJ6</accession>
    <name>Q97UJ6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dehydrogenase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO3015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90482">
        <property type="entry name" value="F90482"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992671.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UJ6"/>
    <dbReference type="FunCoup" id="Q97UJ6">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3015"/>
    <dbReference type="PaxDb" id="273057-SSO3015"/>
    <dbReference type="DNASU" id="1453036"/>
    <dbReference type="EnsemblBacteria" id="AAK43117">
        <property type="protein sequence ID" value="AAK43117"/>
        <property type="gene ID" value="SSO3015"/>
    </dbReference>
    <dbReference type="GeneID" id="1453036"/>
    <dbReference type="KEGG" id="sso:SSO3015"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3108"/>
    <dbReference type="eggNOG" id="arCOG01622">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_1_4_2"/>
    <dbReference type="InParanoid" id="Q97UJ6"/>
    <dbReference type="PhylomeDB" id="Q97UJ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051450">
        <property type="entry name" value="Gfo/Idh/MocA_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377:SF1">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="2">
        <location>
            <begin position="135"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="355" mass="39546" checksum="A7F9B75E7FD0CFAB" modified="2001-10-01" version="1">MKIRYGIIGVGGHGKNRHLIPLIKLKNKVEIVAVADVKKERCEEVSSQFNIKCYLDYMEMVEKESLNAVSIVTPTGLHSRIAINVLNKGVNVLVDKPLGSNLEEVKEVVNTARNKGLKLMVGYWSRFSPALQYGKEIIHNGLLGEPYVAYGYLVRRRGIPGIPTFIDKTLSGGRGALLDIGCYLIDNLLSLLKFRKPISVMGKVYTKFGNVKEEVKFNWGSWDPENFSLDDYAVGLVKLEGDVSLILEVGWSANVSHVEEKSYVRILGDKGGIEGSGHEAIVDISFHSRTENFLTDTKPVLRKVDPYFEMINAFVDSILTDREPPITGEESVTLHSIIDGIYKSSTEDKEVKISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UK8</accession>
    <name>Q97UK8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mandelate racemase/muconate lactonizing enzyme C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2998</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90480">
        <property type="entry name" value="H90480"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992649.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UK8"/>
    <dbReference type="STRING" id="273057.SSO2998"/>
    <dbReference type="PaxDb" id="273057-SSO2998"/>
    <dbReference type="EnsemblBacteria" id="AAK43103">
        <property type="protein sequence ID" value="AAK43103"/>
        <property type="gene ID" value="SSO2998"/>
    </dbReference>
    <dbReference type="GeneID" id="1453022"/>
    <dbReference type="KEGG" id="sso:SSO2998"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3092"/>
    <dbReference type="eggNOG" id="arCOG01168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_3_2_2"/>
    <dbReference type="InParanoid" id="Q97UK8"/>
    <dbReference type="PhylomeDB" id="Q97UK8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047929">
        <property type="term" value="F:gluconate dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03316">
        <property type="entry name" value="MR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034593">
        <property type="entry name" value="DgoD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018110">
        <property type="entry name" value="Mandel_Rmase/mucon_lact_enz_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080:SF2">
        <property type="entry name" value="D-GALACTONATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080">
        <property type="entry name" value="D-GALACTONATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00179">
        <property type="entry name" value="mandelate_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00909">
        <property type="entry name" value="MR_MLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mandelate racemase/muconate lactonizing enzyme C-terminal" evidence="2">
        <location>
            <begin position="171"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="419" mass="47589" checksum="D512968C97876910" modified="2001-10-01" version="1">MELKITDFKVFVAQANFEWAFVRIYSKDLYGTGEAGPAPGLKGMESEFIQLLIGEDAFKVNRIAEKLRYATLYSGTTVYHLISAINIALYDLIGKYLNVPIYKLLGGDKTEIPVYVDAHGGKGLEAINALHLPVNLPWIKEAEVETNRLITTNNPVHGRLSMEKWNEDYSPEAYAKRALKMKNEGYKAMKFDLDVPTPYIDLRRVRNGDLSLKDIDYMVDIVKAVRESVGDEVEIMFDLHWRYNLNTAIRICKALEPYRLRWLEDPMPAIMAVSNYDELKLLTSQCSVPIETGENLYTVYQFKDLLNTGVRVWAPDIVKAGGITEGRRIAELAAMYDIEYSPHNIASPIGTMAHAHVGSIANTFGFVEFHGHDVPFWNEIVKPKRKIIEDGVIKLTDDPGLGIDLDDEVMRKYWPTYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UN2</accession>
    <name>Q97UN2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Quinol oxidase-2, rieske iron-sulfur protein-2 (SoxF)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">soxF</name>
        <name evidence="8" type="ordered locus">SSO2971</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90477">
        <property type="entry name" value="E90477"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992566.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UN2"/>
    <dbReference type="STRING" id="273057.SSO2971"/>
    <dbReference type="PaxDb" id="273057-SSO2971"/>
    <dbReference type="EnsemblBacteria" id="AAK43076">
        <property type="protein sequence ID" value="AAK43076"/>
        <property type="gene ID" value="SSO2971"/>
    </dbReference>
    <dbReference type="GeneID" id="1452995"/>
    <dbReference type="KEGG" id="sso:SSO2971"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3062"/>
    <dbReference type="eggNOG" id="arCOG01720">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074282_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UN2"/>
    <dbReference type="PhylomeDB" id="Q97UN2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03475">
        <property type="entry name" value="Rieske_SoxF_SoxL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005805">
        <property type="entry name" value="Rieske_Fe-S_prot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00162">
        <property type="entry name" value="RIESKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="7">
        <location>
            <begin position="101"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK43076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="252" mass="27288" checksum="A5C8C5E60967F425" modified="2001-10-01" version="1">MMNRRTFLRLYLVVGAAVAIAPLIKPLADYVGYFYNEIGTMSKQYLVANNTDGLSGFPKYRIANIKDIQQQAQSSGCPVYFFAYPLTNEPCFLVDLQALLGQPVPEIPNPYYGQYAGPIGQIKTIQGVGPNNSIYAFSDVCVHLGCQLPAQVLVTSENNPGLDVTNSVLHCPCHGSLYSLMKGGVVVGGPAPRPLPIIFLEYDQNTGDIYAIGTNAPYFSASIPRTTPADNLLYDPRYSYSVPSNPSCSKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UQ0</accession>
    <name>Q97UQ0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Third ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO11549</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90475">
        <property type="entry name" value="C90475"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UQ0"/>
    <dbReference type="PaxDb" id="273057-SSO11549"/>
    <dbReference type="EnsemblBacteria" id="AAK43058">
        <property type="protein sequence ID" value="AAK43058"/>
        <property type="gene ID" value="SSO11549"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11549"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3045"/>
    <dbReference type="eggNOG" id="arCOG09948">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154343_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UQ0"/>
    <dbReference type="PhylomeDB" id="Q97UQ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="77" mass="8756" checksum="D15C5CDD29D25896" modified="2001-10-01" version="1">MINVKLMADRAWIFREDVLVKDVGSARLPVEGSGVKIREGKASSTTLRGVVMEVFFLNFYRDLEILSTRIRTKVLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V28</accession>
    <name>Q97V28_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2807</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90457">
        <property type="entry name" value="H90457"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V28"/>
    <dbReference type="PaxDb" id="273057-SSO2807"/>
    <dbReference type="EnsemblBacteria" id="AAK42919">
        <property type="protein sequence ID" value="AAK42919"/>
        <property type="gene ID" value="SSO2807"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2807"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2893"/>
    <dbReference type="HOGENOM" id="CLU_2353341_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V28"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="118" mass="13684" checksum="B50105FB1C182A93" modified="2001-10-01" version="1">MQFYYMITNFRCPICHRRNTALMIKAQPILYLYPEEHISFHICDKIRLDVCDEFIIVVSNYGTYSSEAINKLREVVDTIKGLIASKATALGNLINEGEKIMRRAENLCRDKKDCNTFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V40</accession>
    <name>Q97V40_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2792</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90456">
        <property type="entry name" value="B90456"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991946.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V40"/>
    <dbReference type="STRING" id="273057.SSO2792"/>
    <dbReference type="PaxDb" id="273057-SSO2792"/>
    <dbReference type="EnsemblBacteria" id="AAK42905">
        <property type="protein sequence ID" value="AAK42905"/>
        <property type="gene ID" value="SSO2792"/>
    </dbReference>
    <dbReference type="GeneID" id="1452824"/>
    <dbReference type="KEGG" id="sso:SSO2792"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2878"/>
    <dbReference type="eggNOG" id="arCOG02187">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_555063_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V40"/>
    <dbReference type="PhylomeDB" id="Q97V40"/>
    <dbReference type="BRENDA" id="2.7.9.2">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050395">
        <property type="entry name" value="4Fe4S_Ferredoxin_RnfB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43560">
        <property type="entry name" value="ION-TRANSLOCATING OXIDOREDUCTASE COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43560:SF1">
        <property type="entry name" value="ION-TRANSLOCATING OXIDOREDUCTASE COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="357"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="389"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="498" mass="55995" checksum="AA24E7C0E03AB9C4" modified="2001-10-01" version="1">MLNAGLIVSKKAREIIGDEILKEVFEEAKLSYVAEMGDYVIDDINNNELKALLLVSENGKERWMEDIDQKLGISPLAILTIPPKWFIGKSKEYIFTLLTAYSLRVELMDLAYRVQPTPTSSVSRRSLLKLKTYEYKPYPVLFDEVHAKREINKAIESCPQGLIVKAPEGPSVGYPEKCSVCGYCSASSYLGYLEIPTATTDQVLSFINTIVRHYRDKQAAILFTDSIIDDIPEGIFPFLMPCTAGVHDSFVLASYAAGITPIVHVSSKCESRDIALKRLDELPSHFPGTNFIISKVRNDEELKKTLSYIKPIQLSRSEIPLEVILQRSRRRALLIWSIEEMSKKVKLNEDDTVPGVYNVEVDPNKCVLCGVCVRACQMLVPELKGSSTLELSYNIPYCIGSQRCVKNCPEKAVIVTGYAKISDLKRKIVNKANVSKCRFCGKPLGSERIKNKVDTLLIQYGFAGTAQYTDVCNECKQKILTKIWLERLLSGKNDTVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VA6</accession>
    <name>Q97VA6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2728</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90447">
        <property type="entry name" value="H90447"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VA6"/>
    <dbReference type="STRING" id="273057.SSO2728"/>
    <dbReference type="PaxDb" id="273057-SSO2728"/>
    <dbReference type="EnsemblBacteria" id="AAK42839">
        <property type="protein sequence ID" value="AAK42839"/>
        <property type="gene ID" value="SSO2728"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2728"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2808"/>
    <dbReference type="eggNOG" id="arCOG03463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034270_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VA6"/>
    <dbReference type="PhylomeDB" id="Q97VA6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF7">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="338"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="416"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="452"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="34"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="520" mass="56476" checksum="D8AD1542B23A830B" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VB8</accession>
    <name>Q97VB8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alcohol dehydrogenase (Zn containing) (Adh-11)</fullName>
            <ecNumber evidence="7">1.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">adh-11</name>
        <name evidence="7" type="ordered locus">SSO2717</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90446">
        <property type="entry name" value="D90446"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988647.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VB8"/>
    <dbReference type="SMR" id="Q97VB8"/>
    <dbReference type="STRING" id="273057.SSO2717"/>
    <dbReference type="PaxDb" id="273057-SSO2717"/>
    <dbReference type="EnsemblBacteria" id="AAK42827">
        <property type="protein sequence ID" value="AAK42827"/>
        <property type="gene ID" value="SSO2717"/>
    </dbReference>
    <dbReference type="GeneID" id="1452746"/>
    <dbReference type="KEGG" id="sso:SSO2717"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2798"/>
    <dbReference type="eggNOG" id="arCOG01459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_0_2"/>
    <dbReference type="InParanoid" id="Q97VB8"/>
    <dbReference type="PhylomeDB" id="Q97VB8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF9">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="6">
        <location>
            <begin position="22"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="317" mass="35833" checksum="E38C448F17A3472D" modified="2001-10-01" version="1">MWKVFFINGNFVKENVRDELPKPNEVKVFPKYIGICGTDKNIYLGKKPVKEPLVLGHEISGITEKGEKVAIFPNYWCGYCNNCRRGFYNSCKNKISIGVNADGGMAEYINVPKDFVFRIPYTLDLKLGALVEPTAVAVAALNKIDKEIEKVIIIGGGSTGTLVSIVAEINGLETFVIEKDSRKEEILKEKGFRTTKELKFDDRVAVIDTINNQESMVLAQSILKNSIARSELIITGLDNEQISLNRDIIVRNEVIIRGSIIYSKDDFLNSIKIVSENQEKFNKIVDKVGYIDNINEWFRKYVIELPNIKVLIKMDSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VE3</accession>
    <name>Q97VE3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90443">
        <property type="entry name" value="B90443"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VE3"/>
    <dbReference type="STRING" id="273057.SSO2684"/>
    <dbReference type="PaxDb" id="273057-SSO2684"/>
    <dbReference type="EnsemblBacteria" id="AAK42801">
        <property type="protein sequence ID" value="AAK42801"/>
        <property type="gene ID" value="SSO2684"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2684"/>
    <dbReference type="eggNOG" id="arCOG08521">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870835_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VE3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="136" mass="15768" checksum="D6474C1ACCFD9E13" modified="2001-10-01" version="1">MRGISEAITVVFLILVTLIAIAIVTIYYLHIVNANQYGLYQELKNYYIDSGQILSVVYYHQINNHSYFYVENIGNLPINITNIYVNHTIVKFIIYNNTNTQIKILYPKVVYVIEVYDNTTNIIIQTSNNNLIQLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VI3</accession>
    <name>Q97VI3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2173 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90438">
        <property type="entry name" value="B90438"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VI3"/>
    <dbReference type="SMR" id="Q97VI3"/>
    <dbReference type="STRING" id="273057.SSO2640"/>
    <dbReference type="PaxDb" id="273057-SSO2640"/>
    <dbReference type="EnsemblBacteria" id="AAK42761">
        <property type="protein sequence ID" value="AAK42761"/>
        <property type="gene ID" value="SSO2640"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2640"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2723"/>
    <dbReference type="eggNOG" id="arCOG07327">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2313779_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VI3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018685">
        <property type="entry name" value="DUF2173"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09941">
        <property type="entry name" value="DUF2173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="12040" checksum="EE9631EF1E6829D8" modified="2001-10-01" version="1">MIEMLDKVPGIIAVFRFREGKLAKIYGQDISMDLDKLSNILVENMRIGETEAKELKFLEPFRGFAMILDDTGVVFVDDYLVITDAKKTNWDLLVKSILKGEVIRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VJ4</accession>
    <name>Q97VJ4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0033 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO10788</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfur carrier protein TusA family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90436">
        <property type="entry name" value="G90436"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992171.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VJ4"/>
    <dbReference type="STRING" id="273057.SSO10788"/>
    <dbReference type="PaxDb" id="273057-SSO10788"/>
    <dbReference type="EnsemblBacteria" id="AAK42750">
        <property type="protein sequence ID" value="AAK42750"/>
        <property type="gene ID" value="SSO10788"/>
    </dbReference>
    <dbReference type="GeneID" id="1452669"/>
    <dbReference type="KEGG" id="sso:SSO10788"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2712"/>
    <dbReference type="eggNOG" id="arCOG02062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2662099_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VJ4"/>
    <dbReference type="PhylomeDB" id="Q97VJ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="entry name" value="TusA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279:SF6">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SirA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="2">
        <location>
            <begin position="8"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="77" mass="8400" checksum="3EC8420A3BFD7EA7" modified="2001-10-01" version="1">MSSLKIYKELDLTSSSCAGPIGELSSVIDELRDGEAVKVILGDEATKKDITLWSKKKGLKIVQESQEGSKYVLLISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VM4</accession>
    <name>Q97VM4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional activator (TenA-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tenA-2</name>
        <name evidence="2" type="ordered locus">SSO2598</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99433">
        <property type="entry name" value="A99433"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989312.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VM4"/>
    <dbReference type="FunCoup" id="Q97VM4">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2598"/>
    <dbReference type="PaxDb" id="273057-SSO2598"/>
    <dbReference type="EnsemblBacteria" id="AAK42720">
        <property type="protein sequence ID" value="AAK42720"/>
        <property type="gene ID" value="SSO2598"/>
    </dbReference>
    <dbReference type="GeneID" id="1454047"/>
    <dbReference type="KEGG" id="sso:SSO2598"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2679"/>
    <dbReference type="eggNOG" id="arCOG01128">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077537_3_2_2"/>
    <dbReference type="InParanoid" id="Q97VM4"/>
    <dbReference type="PhylomeDB" id="Q97VM4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050334">
        <property type="term" value="F:thiaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19365">
        <property type="entry name" value="TenA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027574">
        <property type="entry name" value="Thiaminase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050967">
        <property type="entry name" value="Thiamine_Salvage_TenA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04306">
        <property type="entry name" value="salvage_TenA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198">
        <property type="entry name" value="BIFUNCTIONAL TH2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198:SF2">
        <property type="entry name" value="SI:CH1073-67J19.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiaminase-2/PQQC" evidence="1">
        <location>
            <begin position="13"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="224" mass="26669" checksum="A7E3788630617B8F" modified="2001-10-01" version="1">MMENSEKLWNFISDIYSSILRHPFILELVEGILSRDKFKYYIIQDYLYLREFSKALALLAAKAEDEEQTLLFTTHIQDAIKVEKALHKFYIKEFNLDIEDYEMSPTNLAYTSYLLAVAYSRPFNEVISAVLPCYWIYMKVGKELLKQGSKDKYYQKWIETYGGEEYEKGVRAVLDIVNSLKVSEEEFNKMKIHFRTASIYEYMFWDSAYRLERFPFPTEKNKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VP0</accession>
    <name>Q97VP0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial transposase ISC1058</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90430">
        <property type="entry name" value="H90430"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VP0"/>
    <dbReference type="PaxDb" id="273057-SSO2577"/>
    <dbReference type="EnsemblBacteria" id="AAK42703">
        <property type="protein sequence ID" value="AAK42703"/>
        <property type="gene ID" value="SSO2577"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2577"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2659"/>
    <dbReference type="eggNOG" id="arCOG02754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1551894_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VP0"/>
    <dbReference type="PhylomeDB" id="Q97VP0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053172">
        <property type="entry name" value="Tn903_transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631:SF3">
        <property type="entry name" value="ISSOD12 TRANSPOSASE TNPA_ISSOD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="13"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="172" mass="19602" checksum="B35FE7FC05517EF2" modified="2001-10-01" version="1">MRNMNITFPETSDELEVIVDATGISTNTGGQYIVAKWGKTMDSKFLKIEIVMDNNEFNVVNAEVTSNEVESAVKTVKDLQDKGKKVKKFYGDKAYDVNEVYKTGVEVVVPPKKNASTKRHPARRKAVSEFRKLGYDRWREEKGYGVRWRVESLFSAVKRTFGESVRQVLLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VT8</accession>
    <name>Q97VT8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lipase (LipP-2)</fullName>
            <ecNumber evidence="3">3.1.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">lipP-2</name>
        <name evidence="3" type="ordered locus">SSO2521</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.1.3" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90424">
        <property type="entry name" value="E90424"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988478.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VT8"/>
    <dbReference type="FunCoup" id="Q97VT8">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2521"/>
    <dbReference type="ESTHER" id="sulso-lipP2">
        <property type="family name" value="Hormone-sensitive_lipase_like"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2521"/>
    <dbReference type="EnsemblBacteria" id="AAK42652">
        <property type="protein sequence ID" value="AAK42652"/>
        <property type="gene ID" value="SSO2521"/>
    </dbReference>
    <dbReference type="GeneID" id="1453979"/>
    <dbReference type="KEGG" id="sso:SSO2521"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2598"/>
    <dbReference type="eggNOG" id="arCOG02638">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_6_4_2"/>
    <dbReference type="InParanoid" id="Q97VT8"/>
    <dbReference type="PhylomeDB" id="Q97VT8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000089">
        <property type="entry name" value="Alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF8">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="78"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="311" mass="35509" checksum="E0ECC9CF5EDD8910" modified="2001-10-01" version="1">MPLDPQVKELLTKINLLLPTTPLTPQEFRKVRNELFIKMFNNEKVGLYNIRDMTIPVRNGDIKVRVYFPNQRENLPAVVYYHGGGFVYGNLDTHDSVCRLISKLSNTIIVSVDYRLAPEHKFPTQVYDAYDVVKWLANNGGKLSIDTSKIAVAGDSAGGNLSTVVSILDRDNGENVVKYQIMIYPVVNMLDSSPSMYNYGDGYFLTYERILWYNKQYVKEDSDYYNPLASPILAESHNLPPALIITAEYDPLRDQGEMYAHKLKVSGVKTISLRYNGMIHGFVSFYEYLDTGKEAIHHIASSIRKMFNVFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VW2</accession>
    <name>Q97VW2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CobQ/CobB/MinD/ParA nucleotide binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90421">
        <property type="entry name" value="E90421"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923896.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VW2"/>
    <dbReference type="STRING" id="273057.SSO2492"/>
    <dbReference type="PaxDb" id="273057-SSO2492"/>
    <dbReference type="EnsemblBacteria" id="AAK42628">
        <property type="protein sequence ID" value="AAK42628"/>
        <property type="gene ID" value="SSO2492"/>
    </dbReference>
    <dbReference type="GeneID" id="7808886"/>
    <dbReference type="KEGG" id="sso:SSO2492"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2573"/>
    <dbReference type="eggNOG" id="arCOG07293">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1329528_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VW2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="1">
        <location>
            <begin position="5"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="201" mass="23595" checksum="83EE339255075B3C" modified="2001-10-01" version="1">MSISIFVLSLKGGIGKSRLSYELSRYISKRKFKKVLLIDNDSLSTLSNLLGHYGDGLLDGFDLRSSLKEIDDIFILKLRNKVYFLDNFYDNERLRQLKFVLSRNWDYIIIDNYVGISETNPIIKIIYEFSQLKIGIFLSDDFSLNSTTEYSNSWNYLDSKYVLLTNKPFDVHYIINRTIIDAILNQCEENYDEKFNIEKTN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2025-06-18" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VY9</accession>
    <name>HELS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase Hel308</fullName>
            <ecNumber evidence="1">5.6.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA 3'-5' helicase Hel308</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hel308</name>
        <name type="ordered locus">SSO2462</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. Hel308 subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK42601" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42601.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90418">
        <property type="entry name" value="B90418"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923869.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VY9"/>
    <dbReference type="SMR" id="Q97VY9"/>
    <dbReference type="FunCoup" id="Q97VY9">
        <property type="interactions" value="120"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2462"/>
    <dbReference type="PaxDb" id="273057-SSO2462"/>
    <dbReference type="EnsemblBacteria" id="AAK42601">
        <property type="protein sequence ID" value="AAK42601"/>
        <property type="gene ID" value="SSO2462"/>
    </dbReference>
    <dbReference type="GeneID" id="7806244"/>
    <dbReference type="KEGG" id="sso:SSO2462"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2540"/>
    <dbReference type="eggNOG" id="arCOG00553">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006553_3_0_2"/>
    <dbReference type="InParanoid" id="Q97VY9"/>
    <dbReference type="PhylomeDB" id="Q97VY9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18028">
        <property type="entry name" value="DEXHc_archSki2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00442">
        <property type="entry name" value="Helicase_Hel308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048772">
        <property type="entry name" value="Hel308-like_dom4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053416">
        <property type="entry name" value="Hel308_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022965">
        <property type="entry name" value="Helicase_Hel308"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040935">
        <property type="entry name" value="helicase_Hel308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF10">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE HEL308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21280">
        <property type="entry name" value="Helicase_dom4_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000102112" description="ATP-dependent DNA helicase Hel308">
        <location>
            <begin position="1"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="33"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="1">
        <location>
            <begin position="229"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEAH box" evidence="1">
        <location>
            <begin position="145"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="46"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="708" mass="80500" checksum="D52C24C89541C780" modified="2001-09-26" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VZ3</accession>
    <name>Q97VZ3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90417">
        <property type="entry name" value="F90417"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923865.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VZ3"/>
    <dbReference type="STRING" id="273057.SSO2458"/>
    <dbReference type="PaxDb" id="273057-SSO2458"/>
    <dbReference type="EnsemblBacteria" id="AAK42597">
        <property type="protein sequence ID" value="AAK42597"/>
        <property type="gene ID" value="SSO2458"/>
    </dbReference>
    <dbReference type="GeneID" id="15296729"/>
    <dbReference type="KEGG" id="sso:SSO2458"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2535"/>
    <dbReference type="eggNOG" id="arCOG01147">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083277_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VZ3"/>
    <dbReference type="PhylomeDB" id="Q97VZ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00838">
        <property type="entry name" value="MPP_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051158">
        <property type="entry name" value="Metallophosphoesterase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016538">
        <property type="entry name" value="UCP008292"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31302:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN WITH METALLOPHOSPHOESTERASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31302">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN WITH METALLOPHOSPHOESTERASE DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008292">
        <property type="entry name" value="UCP008292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="228" mass="26271" checksum="9436DF720CC2C1BA" modified="2001-10-01" version="1">MLIISTSDIHSPRYLTEFFLALRELGNIKADLALLAGDLVERGEYLHFTPVYNALKNRVKQIVSIFGNEDFIENRKFYRENYSDIIWLEDEKVEIKIENRKLIIIGSEGVLEKPTLWQTSNGITEDLYMKRLEKITEMSCNSKADIKILLTHYASTFETVFGERKSVYPHLGYRVLEELSTKGKDCLPNIAIHGHAHYAKRTLSVVKSVRVYNVALPANKRIITIHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W40</accession>
    <name>Q97W40_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription regulator, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO10340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90411">
        <property type="entry name" value="G90411"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989431.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W40"/>
    <dbReference type="STRING" id="273057.SSO10340"/>
    <dbReference type="PaxDb" id="273057-SSO10340"/>
    <dbReference type="EnsemblBacteria" id="AAK42550">
        <property type="protein sequence ID" value="AAK42550"/>
        <property type="gene ID" value="SSO10340"/>
    </dbReference>
    <dbReference type="GeneID" id="7797296"/>
    <dbReference type="KEGG" id="sso:SSO10340"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2485"/>
    <dbReference type="eggNOG" id="arCOG01117">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170329_1_3_2"/>
    <dbReference type="InParanoid" id="Q97W40"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="2">
        <location>
            <begin position="9"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="77" mass="8616" checksum="C2A77FB93C3DC6C9" modified="2001-10-01" version="1">MAEVVRAYILVSTTVGKEMEVADMAKKVSGVIRADPVYGEYDVVVEVEAKSSDDLKKVIYEIRRNPNIIRTVTLIVM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-20" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W51</accession>
    <name>IPYR_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="1">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pyrophosphate phospho-hydrolase</fullName>
            <shortName evidence="1">PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ppa</name>
        <name type="ordered locus">SSO2390</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + H2O = 2 phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90410">
        <property type="entry name" value="C90410"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989450.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W51"/>
    <dbReference type="SMR" id="Q97W51"/>
    <dbReference type="FunCoup" id="Q97W51">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2390"/>
    <dbReference type="PaxDb" id="273057-SSO2390"/>
    <dbReference type="EnsemblBacteria" id="AAK42538">
        <property type="protein sequence ID" value="AAK42538"/>
        <property type="gene ID" value="SSO2390"/>
    </dbReference>
    <dbReference type="GeneID" id="1453865"/>
    <dbReference type="GeneID" id="44128113"/>
    <dbReference type="KEGG" id="sso:SSO2390"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2473"/>
    <dbReference type="eggNOG" id="arCOG01711">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073198_1_0_2"/>
    <dbReference type="InParanoid" id="Q97W51"/>
    <dbReference type="PhylomeDB" id="Q97W51"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.80.10:FF:000003">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00209">
        <property type="entry name" value="Inorganic_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002317">
        <property type="entry name" value="PRK01250.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000137560" description="Inorganic pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00209"/>
        </source>
    </evidence>
    <sequence length="172" mass="19695" checksum="A26DCE6107351298" modified="2001-10-01" version="1">MKLGPGKKAPDEINVFIEIPMGSNIKYEYDEEEDIIKVDRVLYTSMVYPFNYGFIPETLEEDGDPLDVLVLGNYSLMPGTVIEARPIGMIYMRDEEGEDAKVIAVPRNKTDPSFSNINDVKDLPEAIRNKIVHFFEHYKELEPNKWVKISGWGSVAEAKERIKKAIERKKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W52</accession>
    <name>Q97W52_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glucosaminyltransferase, intercellular adhesion protein A homolog, putative (IcaA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">icaA</name>
        <name evidence="9" type="ordered locus">SSO2389</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90410">
        <property type="entry name" value="B90410"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989452.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W52"/>
    <dbReference type="STRING" id="273057.SSO2389"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2389"/>
    <dbReference type="EnsemblBacteria" id="AAK42537">
        <property type="protein sequence ID" value="AAK42537"/>
        <property type="gene ID" value="SSO2389"/>
    </dbReference>
    <dbReference type="GeneID" id="1453864"/>
    <dbReference type="KEGG" id="sso:SSO2389"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2472"/>
    <dbReference type="eggNOG" id="arCOG01391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_644993_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W52"/>
    <dbReference type="PhylomeDB" id="Q97W52"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06423">
        <property type="entry name" value="CESA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050321">
        <property type="entry name" value="Glycosyltr_2/OpgH_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43867">
        <property type="entry name" value="CELLULOSE SYNTHASE CATALYTIC SUBUNIT A [UDP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43867:SF2">
        <property type="entry name" value="CELLULOSE SYNTHASE CATALYTIC SUBUNIT A [UDP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="8">
        <location>
            <begin position="135"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="426" mass="49035" checksum="EA9484091395E72F" modified="2001-10-01" version="1">MIREIFEILLFISSFFTSLWILLQAFYYKVSNQNIIQFSTKNDKNSNKRIDIIVAIKDEDEKTIKELINNLSGLDYRFYKVIIVSDDTEETFKKIIESLDKLPDNFVIIRRPENKGRKAGALNFATNISDAEMLVYLDAEARVEKDFLRKISQLDYDAVAFRLKVRDVNTQVQKIYSYTNEFVMNALFKARDKLGLIIFANGSAFGIKRDILRKIGGWKENSVAEDLELGIRLALSNIKVKYVDDITVYTLAPYTHTDLYNQIKRWAYGSGELISYSMRLFKLGIRGIEGFIYSQQWGIYPLYLLLFLIIISIQFILNINYFYVFTSLIPILVSNGIYIALIKPKGDYKSGIVTLIASLIGYIQGIFKVRFKWKVTPKSLVGKEEEILSIKILGIILAIMAYINSLFNNTISSLLIILFSLILLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WE3</accession>
    <name>Q97WE3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">NADH oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO2283</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90398">
        <property type="entry name" value="F90398"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991832.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WE3"/>
    <dbReference type="STRING" id="273057.SSO2283"/>
    <dbReference type="PaxDb" id="273057-SSO2283"/>
    <dbReference type="EnsemblBacteria" id="AAK42445">
        <property type="protein sequence ID" value="AAK42445"/>
        <property type="gene ID" value="SSO2283"/>
    </dbReference>
    <dbReference type="GeneID" id="1453772"/>
    <dbReference type="KEGG" id="sso:SSO2283"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2376"/>
    <dbReference type="eggNOG" id="arCOG01069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003291_1_3_2"/>
    <dbReference type="InParanoid" id="Q97WE3"/>
    <dbReference type="PhylomeDB" id="Q97WE3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050451">
        <property type="term" value="F:CoA-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017758">
        <property type="entry name" value="CoA_disulphide_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050260">
        <property type="entry name" value="FAD-bd_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03385">
        <property type="entry name" value="CoA_CoA_reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429:SF1">
        <property type="entry name" value="NAD(P)H SULFUR OXIDOREDUCTASE (COA-DEPENDENT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="9">
        <location>
            <begin position="3"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="8">
        <location>
            <begin position="321"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="441" mass="49254" checksum="58E5D9C44081FD7B" modified="2001-10-01" version="1">MERLIIIGGGAAGMTAASWVRRLKPNMHVTVFESTKMVSHAPCGIPYFTEGLFDDENLFMTYTPEYFIEKRKINVKTNSKVEEVDLRSRIVVVRENQEKRKYEFDYLLFSTGARPKKINAEGDRIFYVHHPAEASYIRQKLWSFNRIAIIGGGILGIEMAEALRARGKKIVLIHRGRYLLNKMLDEDMGKIVTDKVESEIELKLNESLVSVIEEGRVVITDKGKYDVDATVVAIGVEPNIDLVKDQLKIGQTGAIWADNHMRTSVENVYAAGDSTESINIITKKPDWVPFAPVANKMGFVAGNNIGGKDVTFPGVVGTMITKFEEFIIGKTGVTENEAKRYNIKTVSAIVHHKTRARYYPGSKDIIVKLIAEADTMRIIGAQIVGEEEVLGRLNMMAAVIQKGFTAEDLFFVETGYVPPVNRVWDVVTLAARKLFSGISGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WH4</accession>
    <name>Q97WH4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90394">
        <property type="entry name" value="F90394"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991548.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WH4"/>
    <dbReference type="STRING" id="273057.SSO2245"/>
    <dbReference type="PaxDb" id="273057-SSO2245"/>
    <dbReference type="EnsemblBacteria" id="AAK42413">
        <property type="protein sequence ID" value="AAK42413"/>
        <property type="gene ID" value="SSO2245"/>
    </dbReference>
    <dbReference type="GeneID" id="1453740"/>
    <dbReference type="KEGG" id="sso:SSO2245"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2340"/>
    <dbReference type="eggNOG" id="arCOG05887">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1709216_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WH4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="154" mass="18180" checksum="AFC250903988136E" modified="2001-10-01" version="1">MQNFRELSIDIVLSHKIRNYDQVVLDGTKKRDSCAFFIYGYCKKISPRSKVLASWISNGKIIPHPLFCYLCPFYSLRDDDKTVTVDLFDIYLTYKNLKTQIEKELEFIESRLSEFSFSTSIALRRRREDLIAFLDDISTKSKILLEIIRMSERT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WN4</accession>
    <name>Q97WN4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SAM-dependent methyltransferase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2177</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99387">
        <property type="entry name" value="A99387"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992124.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WN4"/>
    <dbReference type="SMR" id="Q97WN4"/>
    <dbReference type="STRING" id="273057.SSO2177"/>
    <dbReference type="PaxDb" id="273057-SSO2177"/>
    <dbReference type="EnsemblBacteria" id="AAK42352">
        <property type="protein sequence ID" value="AAK42352"/>
        <property type="gene ID" value="SSO2177"/>
    </dbReference>
    <dbReference type="GeneID" id="1453679"/>
    <dbReference type="KEGG" id="sso:SSO2177"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2273"/>
    <dbReference type="eggNOG" id="arCOG01791">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069129_1_1_2"/>
    <dbReference type="InParanoid" id="Q97WN4"/>
    <dbReference type="PhylomeDB" id="Q97WN4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.110">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="2">
        <location>
            <begin position="45"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="243" mass="28844" checksum="7EA4E6DA9AAF2705" modified="2001-10-01" version="1">MSDEWIKIFESDFYVREMIKVWDEGEKWANWIDEVVSKYKLEKKVLDIPCGIGRVSYFLANRGYKVTGIDISERMISMARSNIQNGKFIVGDMRKIKEIIGNEKYDLVINIYNSLGYYSEEDDLKILESLRDSTRKIVVINLDNRDYIIYNRPNEYYTYIPPYLVYSKVEFEQETSRLKIYRKYYLNDKEVGEMIYEQRLYSIHEILALLKKAGLQPIEVLSGYSWKKFSIMDPEMTIIATPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WQ6</accession>
    <name>Q97WQ6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl-CoA C-acetyltransferase (Acetoacetyl-CoA thiolase) (AcaB-3)</fullName>
            <ecNumber evidence="4">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">acaB-3</name>
        <name evidence="4" type="ordered locus">SSO2062</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.9" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90373">
        <property type="entry name" value="G90373"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WQ6"/>
    <dbReference type="FunCoup" id="Q97WQ6">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2062"/>
    <dbReference type="PaxDb" id="273057-SSO2062"/>
    <dbReference type="EnsemblBacteria" id="AAK42246">
        <property type="protein sequence ID" value="AAK42246"/>
        <property type="gene ID" value="SSO2062"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2062"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2141"/>
    <dbReference type="eggNOG" id="arCOG01280">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_2_2_2"/>
    <dbReference type="InParanoid" id="Q97WQ6"/>
    <dbReference type="PhylomeDB" id="Q97WQ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004721">
        <property type="entry name" value="PRK06065.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF7">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE (ACETOACETYL-COA THIOLASE) (ACAB-3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="2">
        <location>
            <begin position="28"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="3">
        <location>
            <begin position="273"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="409" mass="44865" checksum="02CA773D993F791C" modified="2001-10-01" version="1">MAWNSHAYWISRGGGKAMKVNIHLNKRVAIIGAGLTLFRRRLLETPQEIAWEAASKALDEAGLELKDIDCVVIGSAPDAFDGVNLKGEYLSHGAGGIKKPVSRVYVGGATGVMTAITGWYHVASGLCQKVLAVAEEKMSPARPHPQSIFRYIWDPILEKPLNPNLIWIFAMEMHRYMATYGIKKEEIALVSVKNKRNAINNPYAQLGSNITVEDVLKSEVLVWPVQLLDVSPVSDGGAAIVLASEDVARRYTDTPVWVEGVGWTLDNTEWPARDLAYARYVEFAARMAYKMAGIERPNKEIDVAEPYDPFDYKELHHLEALQLAKRGEAPKLLKEGVFDIDGDIPSSPSGGLLGVGNPIAAAGLMKVISIYWQLKGIAGKMQVKRPVHTGVAQAWGDLMQAGTVIVLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WR0</accession>
    <name>Q97WR0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH bat-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90373">
        <property type="entry name" value="C90373"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923762.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WR0"/>
    <dbReference type="STRING" id="273057.SSO2058"/>
    <dbReference type="PaxDb" id="273057-SSO2058"/>
    <dbReference type="DNASU" id="1453569"/>
    <dbReference type="EnsemblBacteria" id="AAK42242">
        <property type="protein sequence ID" value="AAK42242"/>
        <property type="gene ID" value="SSO2058"/>
    </dbReference>
    <dbReference type="GeneID" id="84058137"/>
    <dbReference type="KEGG" id="sso:SSO2058"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2137"/>
    <dbReference type="eggNOG" id="arCOG02272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104910_1_0_2"/>
    <dbReference type="InParanoid" id="Q97WR0"/>
    <dbReference type="PhylomeDB" id="Q97WR0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH bat-type" evidence="1">
        <location>
            <begin position="150"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="210" mass="24353" checksum="EA51F9D1245D94B1" modified="2001-10-01" version="1">MINLIKHVDIVVSHEDCWTSHMPFTAYTINLEVYPHKNYLRSRILIDSMENKVISNLMKTHRSVIKVMKVDRFKGGTYVDFLNKYKGSIAGVLYDKEVLILGNLIEGKDEKWSFVTSSKNLREILTEIESLGKVKRVETTDFNPILYPNLTEWEKKVLTVSYSYGYLDYPRRATADELAKLLGISKVTFLYHLRNAQRKLIAFSIKNILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WR8</accession>
    <name>Q97WR8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4898 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2048</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90372">
        <property type="entry name" value="C90372"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923754.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WR8"/>
    <dbReference type="SMR" id="Q97WR8"/>
    <dbReference type="PaxDb" id="273057-SSO2048"/>
    <dbReference type="EnsemblBacteria" id="AAK42234">
        <property type="protein sequence ID" value="AAK42234"/>
        <property type="gene ID" value="SSO2048"/>
    </dbReference>
    <dbReference type="GeneID" id="1453561"/>
    <dbReference type="KEGG" id="sso:SSO2048"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2129"/>
    <dbReference type="eggNOG" id="arCOG08428">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2091409_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WR8"/>
    <dbReference type="PhylomeDB" id="Q97WR8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032603">
        <property type="entry name" value="DUF4898"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16239">
        <property type="entry name" value="DUF4898"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="13550" checksum="F2736990BBD8CFDC" modified="2001-10-01" version="1">MMEHLSVKELENYLDDDLLKLLKKEDIRHIFLINTKIISNFEKFFSTFLPYSVKYFVVISSDLPVKIIKESLIRAKDTLEVSCYITSKLPQKSMIIIGLQSVSEREESKELSAFRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WT9</accession>
    <name>Q97WT9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2025</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90369">
        <property type="entry name" value="D90369"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923733.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WT9"/>
    <dbReference type="FunCoup" id="Q97WT9">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2025"/>
    <dbReference type="PaxDb" id="273057-SSO2025"/>
    <dbReference type="EnsemblBacteria" id="AAK42211">
        <property type="protein sequence ID" value="AAK42211"/>
        <property type="gene ID" value="SSO2025"/>
    </dbReference>
    <dbReference type="GeneID" id="1453538"/>
    <dbReference type="KEGG" id="sso:SSO2025"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2102"/>
    <dbReference type="eggNOG" id="arCOG00615">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_1_2_2"/>
    <dbReference type="InParanoid" id="Q97WT9"/>
    <dbReference type="PhylomeDB" id="Q97WT9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02803">
        <property type="entry name" value="OYE_like_FMN_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044152">
        <property type="entry name" value="YqjM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303:SF4">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="7">
        <location>
            <begin position="366"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="555" mass="62889" checksum="AA03BB5C9CD31BFA" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WX7</accession>
    <name>Q97WX7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1984</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90364">
        <property type="entry name" value="G90364"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993005.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WX7"/>
    <dbReference type="PaxDb" id="273057-SSO1984"/>
    <dbReference type="EnsemblBacteria" id="AAK42174">
        <property type="protein sequence ID" value="AAK42174"/>
        <property type="gene ID" value="SSO1984"/>
    </dbReference>
    <dbReference type="GeneID" id="1453501"/>
    <dbReference type="KEGG" id="sso:SSO1984"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2059"/>
    <dbReference type="eggNOG" id="arCOG10353">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1745630_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WX7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="149" mass="17077" checksum="C47B18ABA7CEEF0A" modified="2001-10-01" version="1">MIQNVVWDGQYAGTIIQNHYQIVQLNDEWVGRTDPVNNQQYVTFQDFYVIKGQVPIENVTINGQTYYVIDADKINPADIAGFFTYWRWVNNFVTAINTPGTYAAVLPGNSPVFTWENLTGAVAYETRRNGIMGLDWGIVLTQWNYNSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X90</accession>
    <name>Q97X90_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1865</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90349">
        <property type="entry name" value="E90349"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988468.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X90"/>
    <dbReference type="FunCoup" id="Q97X90">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1865"/>
    <dbReference type="PaxDb" id="273057-SSO1865"/>
    <dbReference type="EnsemblBacteria" id="AAK42052">
        <property type="protein sequence ID" value="AAK42052"/>
        <property type="gene ID" value="SSO1865"/>
    </dbReference>
    <dbReference type="GeneID" id="1453379"/>
    <dbReference type="KEGG" id="sso:SSO1865"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1914"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_16_2_2"/>
    <dbReference type="InParanoid" id="Q97X90"/>
    <dbReference type="PhylomeDB" id="Q97X90"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF25">
        <property type="entry name" value="USPA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="3"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="143" mass="15226" checksum="5FDEDB9FB1020146" modified="2001-10-01" version="1">MVFKNIVVAYDGSQNAKRALDVAIDLAKRYEAKLTIIEVIDTSVLAGMGLGPIPGEVINEMYNKAKRDVEEAKEKAVNSGVKNVEAVNIEGDPAAAIMDYAGKTGADLIVTGSRGLSTVKRIFLGSVSSRIVHEAKIPVLVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XF7</accession>
    <name>Q97XF7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8906</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90340">
        <property type="entry name" value="B90340"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923584.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XF7"/>
    <dbReference type="PaxDb" id="273057-SSO8906"/>
    <dbReference type="EnsemblBacteria" id="AAK41977">
        <property type="protein sequence ID" value="AAK41977"/>
        <property type="gene ID" value="SSO8906"/>
    </dbReference>
    <dbReference type="GeneID" id="1454756"/>
    <dbReference type="KEGG" id="sso:SSO8906"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1835"/>
    <dbReference type="eggNOG" id="arCOG07851">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2857279_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XF7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="64" mass="7588" checksum="DC8888690315FE33" modified="2001-10-01" version="1">MGKDLRKRIKKNSEDFYKLYQEGKKKINISNNLLVFLLLSAIEEISKCLIEILLDKCNNPQLRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XG6</accession>
    <name>Q97XG6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8892</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90339">
        <property type="entry name" value="A90339"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988982.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XG6"/>
    <dbReference type="PaxDb" id="273057-SSO8892"/>
    <dbReference type="EnsemblBacteria" id="AAK41968">
        <property type="protein sequence ID" value="AAK41968"/>
        <property type="gene ID" value="SSO8892"/>
    </dbReference>
    <dbReference type="GeneID" id="1454747"/>
    <dbReference type="KEGG" id="sso:SSO8892"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1825"/>
    <dbReference type="HOGENOM" id="CLU_2597914_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XG6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="89" mass="10454" checksum="7C4ABAA19ED17B06" modified="2001-10-01" version="1">MPSKAIFRGKMSKVEEYKIIEHIPANSRTRSIARDAYKETRELYIKIFGNIDTSIWPETPMTCGDSYSGAFVDYDEKKYPEEEYKDPFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-11-27" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XI5</accession>
    <name>Q97XI5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3782 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1752</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90336">
        <property type="entry name" value="E90336"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923572.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XI5"/>
    <dbReference type="STRING" id="273057.SSO1752"/>
    <dbReference type="PaxDb" id="273057-SSO1752"/>
    <dbReference type="EnsemblBacteria" id="AAK41948">
        <property type="protein sequence ID" value="AAK41948"/>
        <property type="gene ID" value="SSO1752"/>
    </dbReference>
    <dbReference type="GeneID" id="1454727"/>
    <dbReference type="KEGG" id="sso:SSO1752"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1795"/>
    <dbReference type="eggNOG" id="arCOG01424">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_965117_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XI5"/>
    <dbReference type="PhylomeDB" id="Q97XI5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38753">
        <property type="entry name" value="SLR1441 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38753:SF1">
        <property type="entry name" value="SLR1441 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="37437" checksum="41FCCF2672F7BCE0" modified="2001-10-01" version="1">MSSERLIDEILKNPQLISALADKVYEKLKDEIVIRKLEENTKSIQALQEAIKRHGDAIVSLQEAVKSLQEEVKRQGEAIVSLQKTVEKQGEAIVSLQEEVKRHGDAIVSLQEAVKSLQEEVKRQGEAIVSLQKTVEKQGEAIVSLQEEVKRHGDAIVSLQEAVKSLQEEVKRQGEAIVSLQEEVKKHSKAILRLAKSQKRLSIELGSFTSRAGKGLERAMLKLYKKSLELHGVDPKRVKHGNIVDTVGIIEKGRSFEVDFYETNDYIYVFEIKNFSDKDVIDQVFVRKKLFSVLYNKPLKVFVVTNYIERDVKEKLEKEGVEIIASHVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XI6</accession>
    <name>Q97XI6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1395</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1751</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90336">
        <property type="entry name" value="D90336"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XI6"/>
    <dbReference type="STRING" id="273057.SSO1751"/>
    <dbReference type="PaxDb" id="273057-SSO1751"/>
    <dbReference type="EnsemblBacteria" id="AAK41947">
        <property type="protein sequence ID" value="AAK41947"/>
        <property type="gene ID" value="SSO1751"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1751"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1794"/>
    <dbReference type="eggNOG" id="arCOG02129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140142_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XI6"/>
    <dbReference type="PhylomeDB" id="Q97XI6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="3"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="159" mass="18730" checksum="0A138F89F6DF8FCA" modified="2001-10-01" version="1">MFFTDESGIHHDPSKVRRLGLYNVPVDYPSRKLNVLASIPLFDGVPCFMFTYSNVNSEIFAEFLRLFRNSGPIVLILDNAKFHKNAYVFSVASQLGITLLFLPPYSPDLNPIELVWKDLKRWVNTYYNLEYTLDSMEEEFYYLFLRECSQTVMKFKKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XL6</accession>
    <name>Q97XL6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1701</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90331">
        <property type="entry name" value="E90331"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988987.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XL6"/>
    <dbReference type="PaxDb" id="273057-SSO1701"/>
    <dbReference type="EnsemblBacteria" id="AAK41908">
        <property type="protein sequence ID" value="AAK41908"/>
        <property type="gene ID" value="SSO1701"/>
    </dbReference>
    <dbReference type="GeneID" id="1454687"/>
    <dbReference type="KEGG" id="sso:SSO1701"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1751"/>
    <dbReference type="eggNOG" id="arCOG03706">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153003_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XL6"/>
    <dbReference type="PhylomeDB" id="Q97XL6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="3"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="129" mass="14511" checksum="0DD3C335DAFE232B" modified="2001-10-01" version="1">MKYYIDTSLAIALVNEKDPNHEVALKTYPNDGKKVISKIVLAEMYSVYSRKLNLTDEELEAIVNYTLSKCGCEIEEVDLGKVIDLAIKISSKVKLKTLDLLHLSASIILDSEILSLDNEILEAKNKIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XP2</accession>
    <name>Q97XP2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF917 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1671</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90328">
        <property type="entry name" value="B90328"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990104.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XP2"/>
    <dbReference type="SMR" id="Q97XP2"/>
    <dbReference type="STRING" id="273057.SSO1671"/>
    <dbReference type="PaxDb" id="273057-SSO1671"/>
    <dbReference type="EnsemblBacteria" id="AAK41881">
        <property type="protein sequence ID" value="AAK41881"/>
        <property type="gene ID" value="SSO1671"/>
    </dbReference>
    <dbReference type="GeneID" id="1454660"/>
    <dbReference type="KEGG" id="sso:SSO1671"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1718"/>
    <dbReference type="eggNOG" id="arCOG03841">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038930_0_1_2"/>
    <dbReference type="InParanoid" id="Q97XP2"/>
    <dbReference type="PhylomeDB" id="Q97XP2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.390.10">
        <property type="entry name" value="CV3147-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1610.10">
        <property type="entry name" value="CV3147-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048350">
        <property type="entry name" value="S-Me-THD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024071">
        <property type="entry name" value="S-Me-THD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010318">
        <property type="entry name" value="S-Me-THD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027479">
        <property type="entry name" value="S-Me-THD_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20906">
        <property type="entry name" value="S-Me-THD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06032">
        <property type="entry name" value="S-Me-THD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160991">
        <property type="entry name" value="CV3147-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-Me-THD N-terminal" evidence="1">
        <location>
            <begin position="7"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="S-Me-THD-like C-terminal" evidence="2">
        <location>
            <begin position="166"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="38589" checksum="7C7B2DCB76EBB10F" modified="2001-10-01" version="1">MRISVNDLYNLAIGSSILGSGGGGNPFLGYKIVKAKMLQMNIDYVEYTDEVDEDRDFIIGVGGMGSPLIGIEKIPAGHEYYRSLMTLTKFLRKEVTKITSIEIGGINSVVPFMASLMSKKPLIDGDYEGRAFPELYMTTMHFAGYRATPLSICDERENCVIIETVDNFRAESIARSITVGFGGRGYISLYPASGKEYINGAILGTVSLALELGKTLTEQGLDEMINLARGKIIFEGKIVDVKRFNIGGFAIGMAKIDGLEEYRGENSEIIFKNEYLGFLKEGKILSISPEIINLIDYNNNIVTSDSLRYGVKVRVLEIPVSNKWKEVGGYEKIKEMVVKEIKKIHQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XQ2</accession>
    <name>Q97XQ2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1657</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90326">
        <property type="entry name" value="H90326"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923552.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XQ2"/>
    <dbReference type="STRING" id="273057.SSO1657"/>
    <dbReference type="PaxDb" id="273057-SSO1657"/>
    <dbReference type="EnsemblBacteria" id="AAK41871">
        <property type="protein sequence ID" value="AAK41871"/>
        <property type="gene ID" value="SSO1657"/>
    </dbReference>
    <dbReference type="GeneID" id="1454650"/>
    <dbReference type="KEGG" id="sso:SSO1657"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1707"/>
    <dbReference type="eggNOG" id="arCOG02730">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153004_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XQ2"/>
    <dbReference type="PhylomeDB" id="Q97XQ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39664">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39664:SF2">
        <property type="entry name" value="NUCLEIC ACID-BINDING PROTEIN, CONTAINING PIN DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="10"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="126" mass="14708" checksum="EC09E852A351178E" modified="2001-10-01" version="1">MRLEEKLQTVVDTNFIIAIIFRDHEKHEEALKEWEIIEKAYLPLISLSELSYFLIKNGVNLEVVNEVISDPKIEIVPNTIEDVRFALANKEKIKGYDDFNDFLILSTAKRLDLPLLTFDKKLKMKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XT3</accession>
    <name>Q97XT3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Polysaccharide biosynthesis related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1624</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90322">
        <property type="entry name" value="H90322"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923526.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XT3"/>
    <dbReference type="STRING" id="273057.SSO1624"/>
    <dbReference type="PaxDb" id="273057-SSO1624"/>
    <dbReference type="EnsemblBacteria" id="AAK41839">
        <property type="protein sequence ID" value="AAK41839"/>
        <property type="gene ID" value="SSO1624"/>
    </dbReference>
    <dbReference type="GeneID" id="1454618"/>
    <dbReference type="KEGG" id="sso:SSO1624"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1667"/>
    <dbReference type="eggNOG" id="arCOG02209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046380_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XT3"/>
    <dbReference type="PhylomeDB" id="Q97XT3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052556">
        <property type="entry name" value="PolySynth_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43424">
        <property type="entry name" value="LOCUS PUTATIVE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43424:SF1">
        <property type="entry name" value="LOCUS PUTATIVE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="306"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="403"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="424"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="469" mass="51930" checksum="58FB26C3853BCDDD" modified="2001-10-01" version="1">MNPIKNTLKSLSVTTTNVIVALIFFIITAKITNPEFFGKVAIIQLLEVVTSAFFYFIPGQIITREISYLYARKEISKSVIGKFLSFPFLVLPLFLILLLFPNYVKLTIPYLFLYLLNGVMGSIMVGMDMFTETAITGNIFLIIRWGISIIAVLSHNIYLFIEIWTLGGIISVSMNYAFLSRKVGLILPSFDFAFLFKHFKEGLPLYLSSSATFLSSQGDRVTTAYLLGSYYLGLYQFSALVAGVPSMVLGALGGVLLPTASFYKALGKDEKKMSSISFRVLSLITFLAVIISIPTAVLVVEKFFPAYISGIKVLILLLLATTLPFPIGSLTNFIIAAKKSLRPFLILSLLNGGLVLLTSFLLIPRIGIIGGAISQAIVSTISSSFVLVYALKEDTFVLGKRESAILFMMPLIAFYEVFVDPPYLDFLLLLSVILLFKTFRIITAEDMKLIDSFLPSKLKFIISLMKAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XU0</accession>
    <name>Q97XU0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1617</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90322">
        <property type="entry name" value="A90322"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XU0"/>
    <dbReference type="STRING" id="273057.SSO1617"/>
    <dbReference type="PaxDb" id="273057-SSO1617"/>
    <dbReference type="EnsemblBacteria" id="AAK41832">
        <property type="protein sequence ID" value="AAK41832"/>
        <property type="gene ID" value="SSO1617"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1617"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1659"/>
    <dbReference type="eggNOG" id="arCOG07850">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1197674_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XU0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="231" mass="26051" checksum="85D200A182B4EA86" modified="2001-10-01" version="1">MFREAKMGIYFNSSNETILFKAECLLYPLLNYTPAIITPVKTSNTVTMLLNPSIVDENNIEIFNESKNATILQGSYIIDHNGSISTFSLSGEENISIKPWTIIIPNDINIREFESIPINFSFNQFMAEFSVNNNPGYLVETSLPLPYGGIIVNNGKALGVNGFGQYVFTSDGKLTISIKLGFITDLLMTAVDIFFYALFIYFFVYKNIFKGLGTEVFRNVITRISRLKSYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XY1</accession>
    <name>Q97XY1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1577</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90316">
        <property type="entry name" value="E90316"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923478.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XY1"/>
    <dbReference type="STRING" id="273057.SSO1577"/>
    <dbReference type="PaxDb" id="273057-SSO1577"/>
    <dbReference type="EnsemblBacteria" id="AAK41788">
        <property type="protein sequence ID" value="AAK41788"/>
        <property type="gene ID" value="SSO1577"/>
    </dbReference>
    <dbReference type="GeneID" id="1454567"/>
    <dbReference type="KEGG" id="sso:SSO1577"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1612"/>
    <dbReference type="eggNOG" id="arCOG02181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044170_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XY1"/>
    <dbReference type="PhylomeDB" id="Q97XY1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF1">
        <property type="entry name" value="FERREDOXIN III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="108"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="142"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="294"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="327"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="455" mass="51308" checksum="9749A79FC8ED7F4B" modified="2001-10-01" version="1">MTSLFYSPLIKYRVDVLPSSELKKENINTKALVVIGDGINREKISEDLELNPLLVRIVGKDSSKEEVEYNKVVLENAWLADLAPVEEIKSIDRRSLLRGEVKKAKKVDKPIYLSEYCNGLYKACNVCEFSCPYNAIKVDKKTGVNIDYTKCTSCGLCVASCPVSAIQFPSLSQNSIFELAKVKGEKRITCYRNTKNRGVKIPCLAMLSEVDIVLLRGSGNLTFECVGCELQDNLKDFIEVIKEYNERIGGISFYSPSEKIEAKETKELNTTPQSFYNRAEARRNISDELPYILFDVSIDNNRCTLCESCVNWCPTSAIMLRRSSGVEEIDFDPMKCIGCNICVNVCPESCKLEEGKTSEIPPNIASLTKVIKVEKSKSVNKEVRKLVGDELVRCRVCGAPIGSRKSLNHVKKIMIEKGASCEDEWLERCPKHRAEYAFQKQFSFNARFKPRGDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XY8</accession>
    <name>Q97XY8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Third ORF in transposon ISC1160</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO8386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK67523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XY8"/>
    <dbReference type="STRING" id="273057.SSO8386"/>
    <dbReference type="PaxDb" id="273057-SSO8386"/>
    <dbReference type="EnsemblBacteria" id="AAK67523">
        <property type="protein sequence ID" value="AAK67523"/>
        <property type="gene ID" value="SSO8386"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8386"/>
    <dbReference type="eggNOG" id="arCOG07929">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192666_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XY8"/>
    <dbReference type="PhylomeDB" id="Q97XY8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK67523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="68" mass="8152" checksum="B095A2E443E90268" modified="2001-10-01" version="1">MVFRLYNKVNPIETSYRNIKSFLPVISSTKLAFRKLMFMIALYFLLTIFKDVKREDFYSSFYYINTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XZ8</accession>
    <name>Q97XZ8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1491</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO8359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90314">
        <property type="entry name" value="F90314"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XZ8"/>
    <dbReference type="STRING" id="273057.SSO8359"/>
    <dbReference type="PaxDb" id="273057-SSO8359"/>
    <dbReference type="EnsemblBacteria" id="AAK41773">
        <property type="protein sequence ID" value="AAK41773"/>
        <property type="gene ID" value="SSO8359"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8359"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1593"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153635_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XZ8"/>
    <dbReference type="PhylomeDB" id="Q97XZ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF17">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="98" mass="10979" checksum="DDFFD61AA7FEB91A" modified="2001-10-01" version="1">MISAATIYAEFGDVSRFSSSKAARAYAGFAPRTKQSGESESHSGMGNKYLRRAFFLVARVARKSERFKEYYDRLVSKWKSATKLVLLVGSLLVFVITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y10</accession>
    <name>Q97Y10_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S58 DmpA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S58 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90313">
        <property type="entry name" value="B90313"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992935.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y10"/>
    <dbReference type="STRING" id="273057.SSO1540"/>
    <dbReference type="PaxDb" id="273057-SSO1540"/>
    <dbReference type="EnsemblBacteria" id="AAK41761">
        <property type="protein sequence ID" value="AAK41761"/>
        <property type="gene ID" value="SSO1540"/>
    </dbReference>
    <dbReference type="GeneID" id="1454540"/>
    <dbReference type="KEGG" id="sso:SSO1540"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1578"/>
    <dbReference type="eggNOG" id="arCOG07260">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044458_1_0_2"/>
    <dbReference type="InParanoid" id="Q97Y10"/>
    <dbReference type="PhylomeDB" id="Q97Y10"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02252">
        <property type="entry name" value="nylC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.70.12">
        <property type="entry name" value="L-amino peptidase D-ALA esterase/amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005321">
        <property type="entry name" value="Peptidase_S58_DmpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36512:SF3">
        <property type="entry name" value="BLR5678 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36512">
        <property type="entry name" value="D-AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03576">
        <property type="entry name" value="Peptidase_S58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="DmpA/ArgJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="30344" checksum="4F0715F67B1C4BD9" modified="2001-10-01" version="1">MKIGINGIKVGGYTDEKSKSGVTVILSEIENNTAGISQRGGSPATLGTDLLRPKHRGNQSVNAIVLTGRSVFGIRVVDAVLMKLFELGVGFKISQNLKIPIVVSASIFDFYDNTIMPTPDWGYNAMSNLGYDIPIGRYWAGRGATVGKLKGIKYAKPSGQGYYEIENNNLKIGVISVVNSIGNIYDEKGELLVGEETEEFKINTPGTTLGVVITNSKLNNSDACRVASSAENGFSSVIKPYNLSLDGDTVFTIATNEMEVPVDKVIALTYEASRESVLSIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y59</accession>
    <name>Q97Y59_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposon ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1484</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99307">
        <property type="entry name" value="A99307"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923426.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y59"/>
    <dbReference type="STRING" id="273057.SSO1484"/>
    <dbReference type="PaxDb" id="273057-SSO1484"/>
    <dbReference type="EnsemblBacteria" id="AAK41712">
        <property type="protein sequence ID" value="AAK41712"/>
        <property type="gene ID" value="SSO1484"/>
    </dbReference>
    <dbReference type="GeneID" id="1454491"/>
    <dbReference type="KEGG" id="sso:SSO1484"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1523"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066381_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y59"/>
    <dbReference type="PhylomeDB" id="Q97Y59"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="3">
        <location>
            <begin position="194"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="316" mass="35926" checksum="43B2FEF43695E17C" modified="2001-10-01" version="1">MEAPVAGIDVSKDKLVVYFQGKYYEFQNNKQGFEGVKQVLPKGCKVAIESTGIYHINLAKYLGKEYDVRIINPLILKKFKDIRGKKSDKNDAKKLAELVISMGSEFTTSEAKELTSQWDFITRSIVRVKNRLRRDLILLGYKDSLSKKNLNEVLRGEDNIVLSEVRFLLDELERLEVRKREIEEELEDLVPKDSLIFTIPGIGRTLGCIILARVGDVKRFGDKKRFVAYCGLDPLVESSGKSVVSRGISRRGDAVLRRAFYLAALTAIKVNPIIKRFYEEHKGNLKGKKLITACARKLAVITWAVLYYNKPFDASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y60</accession>
    <name>Q97Y60_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Third part of one of two inversely orientated ORFs in ISC1043</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8195</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90306">
        <property type="entry name" value="H90306"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y60"/>
    <dbReference type="STRING" id="273057.SSO8195"/>
    <dbReference type="PaxDb" id="273057-SSO8195"/>
    <dbReference type="EnsemblBacteria" id="AAK41711">
        <property type="protein sequence ID" value="AAK41711"/>
        <property type="gene ID" value="SSO8195"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8195"/>
    <dbReference type="HOGENOM" id="CLU_3113256_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y60"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="50" mass="5662" checksum="1967D411CFE597CC" modified="2001-10-01" version="1">MKKEYTKFSLPLSLPWISLFHSCNAWSLLGNSPSMVASILRSSTVFESRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y71</accession>
    <name>Q97Y71_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1471</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90305">
        <property type="entry name" value="C90305"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993100.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y71"/>
    <dbReference type="STRING" id="273057.SSO1471"/>
    <dbReference type="PaxDb" id="273057-SSO1471"/>
    <dbReference type="DNASU" id="1454477"/>
    <dbReference type="EnsemblBacteria" id="AAK41698">
        <property type="protein sequence ID" value="AAK41698"/>
        <property type="gene ID" value="SSO1471"/>
    </dbReference>
    <dbReference type="GeneID" id="1454477"/>
    <dbReference type="KEGG" id="sso:SSO1471"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1508"/>
    <dbReference type="eggNOG" id="arCOG01203">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131320_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y71"/>
    <dbReference type="PhylomeDB" id="Q97Y71"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052548">
        <property type="entry name" value="Type_VII_TA_antitoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933:SF1">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE MNTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="155" mass="18420" checksum="F69B4EAFFAFFA0F7" modified="2001-10-01" version="1">MEKEIVEAFLKVYGDNLVSIVLFGSYARGDQRKDSDIDLLIVLDKIEDRYEVFKKFFEVEKILDLTVYKELRQKGYNPYVSPIFLDVEKATKFRPLYIDIVFDAKILYDKNDVMKNTFERVRKRLEELGAVRKKKGRIYYVILTKVKPGEVIKYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-05-16" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YC1</accession>
    <name>CAS1A_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRISPR-associated endonuclease Cas1 1</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cas1-1</name>
        <name type="ordered locus">SSO1405</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer, forms a heterotetramer with a Cas2 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated endonuclease Cas1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99298">
        <property type="entry name" value="A99298"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YC1"/>
    <dbReference type="SMR" id="Q97YC1"/>
    <dbReference type="STRING" id="273057.SSO1405"/>
    <dbReference type="PaxDb" id="273057-SSO1405"/>
    <dbReference type="EnsemblBacteria" id="AAK41640">
        <property type="protein sequence ID" value="AAK41640"/>
        <property type="gene ID" value="SSO1405"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1405"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1421"/>
    <dbReference type="eggNOG" id="arCOG01452">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052779_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YC1"/>
    <dbReference type="PhylomeDB" id="Q97YC1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099048">
        <property type="term" value="P:CRISPR-cas system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09636">
        <property type="entry name" value="Cas1_I-II-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.920">
        <property type="entry name" value="CRISPR-associated endonuclease Cas1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.20">
        <property type="entry name" value="CRISPR-associated endonuclease Cas1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01470">
        <property type="entry name" value="Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050646">
        <property type="entry name" value="Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002729">
        <property type="entry name" value="CRISPR-assoc_Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042206">
        <property type="entry name" value="CRISPR-assoc_Cas1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042211">
        <property type="entry name" value="CRISPR-assoc_Cas1_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00287">
        <property type="entry name" value="cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34353">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDONUCLEASE CAS1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34353:SF2">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDONUCLEASE CAS1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01867">
        <property type="entry name" value="Cas_Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000417112" description="CRISPR-associated endonuclease Cas1 1">
        <location>
            <begin position="1"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01470"/>
        </source>
    </evidence>
    <sequence length="352" mass="41079" checksum="AAA8F695D88DEB73" modified="2001-10-01" version="1">MVHKVSQREFSRCYKRNCDCICRKPKQRRNVYLNYSLELLITIFKEVIPNLPMDKKIAFVKDYGAYLKVEKGLITCKIKNQVKWSIAPTELHSIVVLTNSSISSEVVKVANEYGIEIVFFNKHEPYAKLIPAKYAGSFKVWLKQLTAWKRRKVEFAKAFIYGKVHNQWVTLRYYERKYGYNLTSQELDRLAREITFVNTAEEVMQKEAEAAKVYWRGVKSLLPKSLGFKGRMKRVSDNLDPFNRALNIGYGMLRKVVWGAVISVGLNPYIGFLHKFRSGRISLVFDLMEEFRSPFVDRKLIGLARESADKVTDLKTVYSLFSDVKEDEIYTQARRLVNAILNDEEYRPYLAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2013-05-01" modified="2025-04-09" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YD4</accession>
    <name>CSA1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated exonuclease Csa1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">csa1</name>
        <name type="ordered locus">SSO1391</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="PLoS ONE" volume="7" first="E47232" last="E47232">
            <title>The CRISPR associated protein Cas4 is a 5' to 3' DNA exonuclease with an iron-sulfur cluster.</title>
            <authorList>
                <person name="Zhang J."/>
                <person name="Kasciukovic T."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="23056615"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0047232"/>
        </citation>
        <scope>POSSIBLE COFACTOR</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2013" name="J. Am. Chem. Soc." volume="135" first="17476" last="17487">
            <title>Toroidal structure and DNA cleavage by the CRISPR-associated [4Fe-4S] cluster containing Cas4 nuclease SSO0001 from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Lemak S."/>
                <person name="Beloglazova N."/>
                <person name="Nocek B."/>
                <person name="Skarina T."/>
                <person name="Flick R."/>
                <person name="Brown G."/>
                <person name="Popovic A."/>
                <person name="Joachimiak A."/>
                <person name="Savchenko A."/>
                <person name="Yakunin A.F."/>
            </authorList>
            <dbReference type="PubMed" id="24171432"/>
            <dbReference type="DOI" id="10.1021/ja408729b"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>MUTAGENESIS OF GLU-195</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2014" name="Nucleic Acids Res." volume="42" first="11144" last="11155">
            <title>The CRISPR-associated Cas4 protein Pcal_0546 from Pyrobaculum calidifontis contains a [2Fe-2S] cluster: crystal structure and nuclease activity.</title>
            <authorList>
                <person name="Lemak S."/>
                <person name="Nocek B."/>
                <person name="Beloglazova N."/>
                <person name="Skarina T."/>
                <person name="Flick R."/>
                <person name="Brown G."/>
                <person name="Joachimiak A."/>
                <person name="Savchenko A."/>
                <person name="Yakunin A.F."/>
            </authorList>
            <dbReference type="PubMed" id="25200083"/>
            <dbReference type="DOI" id="10.1093/nar/gku797"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF CYS-52; TYR-59; HIS-80; ASP-184; GLU-195; LYS-197; TYR-201; TYR-212; GLU-217; CYS-230; CYS-243; ARG-260; CYS-283 AND CYS-289</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3 4">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). A ssDNA exonuclease that has both 5' to 3' and 3' to 5' activity, yielding 5'-OH and 3'-phosphate groups. Has Mn(2+)-dependent endonuclease activity on circular ssDNA. Can unwind dsDNA; unwinding does not require ATP.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="3 4">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="3 4">Mn(2+) required for nuclease activity. Can also utilize Co(2+) and to a lesser extent Mg(2+) or Ni(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 5 6 7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2 5 6 7">Binds 1 [4Fe-4S] cluster per subunit. Not required for nuclease activity, since mutation of the Cys residues leads to a colorless but active protein.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="4">Optimum pH is 7-9.</text>
        </phDependence>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CRISPR-associated protein Csa1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90296">
        <property type="entry name" value="D90296"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YD4"/>
    <dbReference type="STRING" id="273057.SSO1391"/>
    <dbReference type="PaxDb" id="273057-SSO1391"/>
    <dbReference type="EnsemblBacteria" id="AAK41627">
        <property type="protein sequence ID" value="AAK41627"/>
        <property type="gene ID" value="SSO1391"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1391"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1407"/>
    <dbReference type="eggNOG" id="arCOG04195">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_905009_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YD4"/>
    <dbReference type="PhylomeDB" id="Q97YD4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045145">
        <property type="term" value="F:single-stranded DNA 5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009260">
        <property type="entry name" value="CRISPR-ass_Csa1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01896">
        <property type="entry name" value="cas_AF1879"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06023">
        <property type="entry name" value="Csa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009226">
        <property type="entry name" value="UCP009226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000422231" description="CRISPR-associated exonuclease Csa1">
        <location>
            <begin position="1"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Nearly wild-type protein." evidence="4">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect." evidence="4">
        <original>Y</original>
        <variation>A</variation>
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No exonuclease, endonuclease, or DNA unwinding activity." evidence="4">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No exonuclease, endonuclease, or DNA unwinding activity." evidence="4">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No exonuclease, endonuclease, or DNA unwinding activity." evidence="3 4">
        <original>E</original>
        <variation>A</variation>
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No exonuclease, endonuclease, or DNA unwinding activity." evidence="4">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect." evidence="4">
        <original>Y</original>
        <variation>A</variation>
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No exonuclease, endonuclease, or DNA unwinding activity." evidence="4">
        <original>Y</original>
        <variation>A</variation>
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduced exonuclease and DNA unwinding, no endonuclease activity." evidence="4">
        <original>E</original>
        <variation>A</variation>
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect." evidence="4">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="230"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect." evidence="4">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="243"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Wild-type exonuclease, reduced DNA unwinding, no endonuclease activity." evidence="4">
        <original>R</original>
        <variation>A</variation>
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Wild-type exonuclease and DNA unwinding, reduced endonuclease activity." evidence="4">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="283"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Wild-type exonuclease and DNA unwinding, reduced endonuclease activity." evidence="4">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q97TX9"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="24171432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="25200083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="23056615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="6">
        <source>
            <dbReference type="PubMed" id="24171432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="7">
        <source>
            <dbReference type="PubMed" id="25200083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="8"/>
    <sequence length="291" mass="33491" checksum="BE68EC7DC4F5AC0B" modified="2001-10-01" version="1">MFFTHSDMLLLSKRIKKLPKNVDEELRGWNWSEPPVYTRSLSQVSISEMVYCSTLRNVYLKVKGFRGEIGRQILQGSLIHTIYAIGIEAIKRFIYSRESIDGSTLRTLMGDEFYSLLKDLREEEGIYAKVLWDHITNIYSAELDRVRSKFTNLTRDSLVSQVVPFYVEFPVDGSLLGLTNLRVDAFIPHLPLIAEMKTGKYRYTHELSLAGYALAIESQYEIPIDFGYLCYVTVTEKEVKNNCKLIPISDSLRSEFLDMRDKAQDIMDKGVDPGIAKDCESDCMFYKVCHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YG9</accession>
    <name>Q97YG9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, marR family, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1352</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2009" name="J. Mol. Biol." volume="388" first="559" last="569">
            <title>Structural analysis of BldR from Sulfolobus solfataricus provides insights into the molecular basis of transcriptional activation in Archaea by MarR family proteins.</title>
            <authorList>
                <person name="Di Fiore A."/>
                <person name="Fiorentino G."/>
                <person name="Vitale R.M."/>
                <person name="Ronca R."/>
                <person name="Amodeo P."/>
                <person name="Pedone C."/>
                <person name="Bartolucci S."/>
                <person name="De Simone G."/>
            </authorList>
            <dbReference type="PubMed" id="19298823"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2009.03.030"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90291">
        <property type="entry name" value="E90291"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923345.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3F3X">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A=1-144"/>
    </dbReference>
    <dbReference type="PDBsum" id="3F3X"/>
    <dbReference type="AlphaFoldDB" id="Q97YG9"/>
    <dbReference type="SMR" id="Q97YG9"/>
    <dbReference type="STRING" id="273057.SSO1352"/>
    <dbReference type="PaxDb" id="273057-SSO1352"/>
    <dbReference type="EnsemblBacteria" id="AAK41588">
        <property type="protein sequence ID" value="AAK41588"/>
        <property type="gene ID" value="SSO1352"/>
    </dbReference>
    <dbReference type="GeneID" id="1454367"/>
    <dbReference type="KEGG" id="sso:SSO1352"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1358"/>
    <dbReference type="eggNOG" id="arCOG03181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_27_4_2"/>
    <dbReference type="InParanoid" id="Q97YG9"/>
    <dbReference type="PhylomeDB" id="Q97YG9"/>
    <dbReference type="EvolutionaryTrace" id="Q97YG9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039422">
        <property type="entry name" value="MarR/SlyA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33164:SF43">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REPRESSOR YETL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33164">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, MARR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="5" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="3F3X"/>
        </source>
    </evidence>
    <sequence length="144" mass="16485" checksum="F0D22359B9ED067C" modified="2001-10-01" version="1">MQKIDEKLQLMNTIAKIYRGSIKEFNNRLGKLMNLSYLDFSILKATSEEPRSMVYLANRYFVTQSAITAAVDKLEAKGLVRRIRDSKDRRIVIVEITPKGRQVLLEANEVLRNLVNEMLSDVENVEELLEGLNKILSRIGSSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YL8</accession>
    <name>Q97YL8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Metabolite transport related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1305</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90285">
        <property type="entry name" value="F90285"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923298.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YL8"/>
    <dbReference type="FunCoup" id="Q97YL8">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1305"/>
    <dbReference type="PaxDb" id="273057-SSO1305"/>
    <dbReference type="EnsemblBacteria" id="AAK41541">
        <property type="protein sequence ID" value="AAK41541"/>
        <property type="gene ID" value="SSO1305"/>
    </dbReference>
    <dbReference type="GeneID" id="7809229"/>
    <dbReference type="KEGG" id="sso:SSO1305"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1305"/>
    <dbReference type="eggNOG" id="arCOG02684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_2"/>
    <dbReference type="InParanoid" id="Q97YL8"/>
    <dbReference type="PhylomeDB" id="Q97YL8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001252">
        <property type="entry name" value="Major facilitator superfamily MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="315"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="20"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="463" mass="50503" checksum="6F3A7B59FE0C5635" modified="2001-10-01" version="1">MSEPSDPLKALDEAKLSSTHYRWTILSALADFLDAGAIVAGAASVSIWVSLFHLNSLLLGIIAALSPNAFAAGIGAWIAGPLGDRYGRKAIYTYDLIVYAIGAIIILSAINYYMVIIGYTIVGLAVGVDVPTSWSLIAELAPKRNRGRLMSFTNLFWYIGPIIILLLGIGTVPLGVNSFRVLFGFLTIVAIVTWVLRRGIAESARWGLVKGKEEVVKQALVQLGQSVSVIPKAETKGKWSDMFKYWKGLAFMIPIYIFWGVPAGTFGFFLPFLIEDLSIKSSVVGDLVQIAWFVTAIIGVVGVYMQLSDKVNRKLLYAVSSLICAIGFALPIGLPFKILWVALFNVFLFGFGHGMALWPQTRVWSTELFPTEIRNTAQGFVWGWMRIALGIWSIFVPSIITVIGYSAIAAVATSFFILNIILGLLVGPDTHGKSLEVVIKDFYGGKVPVSKVVEVNTRPLGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YP8</accession>
    <name>Q97YP8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Oligo/dipeptide transport, ATP binding protein (DppD-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dppD-1</name>
        <name evidence="10" type="ordered locus">SSO1276</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90282">
        <property type="entry name" value="B90282"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YP8"/>
    <dbReference type="FunCoup" id="Q97YP8">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1276"/>
    <dbReference type="PaxDb" id="273057-SSO1276"/>
    <dbReference type="EnsemblBacteria" id="AAK41513">
        <property type="protein sequence ID" value="AAK41513"/>
        <property type="gene ID" value="SSO1276"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1276"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1273"/>
    <dbReference type="eggNOG" id="arCOG00181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="InParanoid" id="Q97YP8"/>
    <dbReference type="PhylomeDB" id="Q97YP8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000016">
        <property type="entry name" value="Oligopeptide ABC transporter ATP-binding component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050388">
        <property type="entry name" value="ABC_Ni/Peptide_Import"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43297:SF14">
        <property type="entry name" value="ATPASE AAA-TYPE CORE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43297">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT ATP-BINDING PROTEIN APPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="9"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK41513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="316" mass="35875" checksum="486F80752012972B" modified="2001-10-01" version="1">MRDRMLLEVRNLKVYFYTKRGIVKAVDGVNLYLDKGEIVGLAGESGSGKSTIGYAIMRLVPFPGKIESGEILFKGENILKLDEEAFRKNYRWKKIAMVFQGSMSGFTPVFKIKDQIIEVLRIHGWNGDYEERIKELFKMVNMDPQLAEKYPHELSGGQKQRAFIAMALALNPEVLIADEPTTALDVMVQEHILNLLKKLRKELNLSIIFITHDLALLSEISDRDYTLYAGKVMESGPSEVIFKKPRHPYTSLLIESIATLDKDIIKGIPGAMPDLSSPPLGCRFNTRCPFARDICFKEEPKMKTFSDGDEVACWLY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2025-06-18" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YR6</accession>
    <name>Y1253_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Putative esterase SSO1253</fullName>
            <ecNumber>3.1.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO1253</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.-"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90279">
        <property type="entry name" value="E90279"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YR6"/>
    <dbReference type="SMR" id="Q97YR6"/>
    <dbReference type="FunCoup" id="Q97YR6">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1253"/>
    <dbReference type="EnsemblBacteria" id="AAK41492">
        <property type="protein sequence ID" value="AAK41492"/>
        <property type="gene ID" value="SSO1253"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1253"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1248"/>
    <dbReference type="eggNOG" id="arCOG00777">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_3_3_2"/>
    <dbReference type="InParanoid" id="Q97YR6"/>
    <dbReference type="PhylomeDB" id="Q97YR6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000113">
        <property type="entry name" value="Thioesterase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660:SF1">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660">
        <property type="entry name" value="THIOESTERASE SUPERFAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156691" description="Putative esterase SSO1253">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="150" mass="16816" checksum="9C54934AFBAF3605" modified="2001-10-01" version="1">MKNKNLFPMLSVSEVNELLKQEEIFNFIGIEFEKLEKGYSRLKFNFNEKLTRIGGILHGGVVFSAVDYAGSYAVRTLDKVKDGVTAELKINFLKPMKEGPFTVEPRVISEGKRLVVVDISAYDGNSNLCAKALGTWVVYRETNSETQLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YS4</accession>
    <name>Q97YS4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">First ORF in partial transposon ISC1491</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90278">
        <property type="entry name" value="D90278"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YS4"/>
    <dbReference type="STRING" id="273057.SSO1240"/>
    <dbReference type="PaxDb" id="273057-SSO1240"/>
    <dbReference type="EnsemblBacteria" id="AAK41483">
        <property type="protein sequence ID" value="AAK41483"/>
        <property type="gene ID" value="SSO1240"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1240"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1237"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098916_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YS4"/>
    <dbReference type="PhylomeDB" id="Q97YS4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="191" mass="22089" checksum="305390F43C7D5AF6" modified="2001-10-01" version="1">MKSKNFPEILMESTGPYFFYLHEELTEKGYKVTVINPLHLKEVFGKKTDKLDAQRLAKAFILGAVKGSYIPTGEIRELRELTRYRESLMRKITQVKNEIRKFLEMAGYKIEPFDKRGMALLEKLSRGEGLSKEERDELKEKLGRSLNDAEKLALKQLVDLLKSLEAMVKEVEDMIISKIPQPVVELSRELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YT5</accession>
    <name>Q97YT5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acetoacetate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1222</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90276">
        <property type="entry name" value="F90276"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YT5"/>
    <dbReference type="STRING" id="273057.SSO1222"/>
    <dbReference type="PaxDb" id="273057-SSO1222"/>
    <dbReference type="EnsemblBacteria" id="AAK41469">
        <property type="protein sequence ID" value="AAK41469"/>
        <property type="gene ID" value="SSO1222"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1222"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1223"/>
    <dbReference type="eggNOG" id="arCOG04489">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091354_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YT5"/>
    <dbReference type="PhylomeDB" id="Q97YT5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.400.10">
        <property type="entry name" value="Acetoacetate decarboxylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010451">
        <property type="entry name" value="Acetoacetate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023375">
        <property type="entry name" value="ADC_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06314">
        <property type="entry name" value="ADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160104">
        <property type="entry name" value="Acetoacetate decarboxylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="34024" checksum="AFDAB37C4B12947E" modified="2001-10-01" version="1">MVFLSEIGKLETSLMECLFSRLKVKVPMTKNNSDFTLPPTNSGKSQIVFQPPWYYGVTYIGAHVKFTKESAEKVIPGFLSTDGEGWVYIAEFISTSEDNWDYMYQDPDLVQYMEGAIGLKVNFEGKNYLYFPFMWVDKDWALVRGWLDGYPKKIAKIAMTKLHPLLPKYNKPESGLRLGGYVVRGGGVMFRMQVELEGRTDSLPLKSFGPFLNIRRFPSRGEGEVDLYEIVSRVRDESKYGEIWKGKANVELGGYVNDEVSLLEVERTIGGYYYTLYFKVTTTQLISKKIEETISLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YV7</accession>
    <name>Q97YV7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90273">
        <property type="entry name" value="E90273"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923238.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YV7"/>
    <dbReference type="PaxDb" id="273057-SSO1196"/>
    <dbReference type="EnsemblBacteria" id="AAK41444">
        <property type="protein sequence ID" value="AAK41444"/>
        <property type="gene ID" value="SSO1196"/>
    </dbReference>
    <dbReference type="GeneID" id="34442672"/>
    <dbReference type="KEGG" id="sso:SSO1196"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1195"/>
    <dbReference type="eggNOG" id="arCOG03169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387634_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YV7"/>
    <dbReference type="PhylomeDB" id="Q97YV7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301:SF8">
        <property type="entry name" value="ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301">
        <property type="entry name" value="DNA-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="196" mass="22049" checksum="2D905C41BDB13B31" modified="2001-10-01" version="1">MIFDLHPKETIEELVGREKEVEHCISQILSKNWIIIGGQREIGKTSLMKVVINEIKKREQIAGIYINLRGVRSLNSLLTILVSEINKEKISWRFKVNINFLITSAGIEIRGGSKRRVVNSLIELLNSSDEIVIAFDEVQELSFASKQFLDILGNVYATNPKVHMIFSGSYVGLVKALLSPPSDSPLHGRPPTEIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YW2</accession>
    <name>Q97YW2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1189</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90272">
        <property type="entry name" value="G90272"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YW2"/>
    <dbReference type="STRING" id="273057.SSO1189"/>
    <dbReference type="PaxDb" id="273057-SSO1189"/>
    <dbReference type="DNASU" id="1454217"/>
    <dbReference type="EnsemblBacteria" id="AAK41438">
        <property type="protein sequence ID" value="AAK41438"/>
        <property type="gene ID" value="SSO1189"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1189"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1189"/>
    <dbReference type="eggNOG" id="arCOG01933">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044590_4_0_2"/>
    <dbReference type="InParanoid" id="Q97YW2"/>
    <dbReference type="PhylomeDB" id="Q97YW2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01292">
        <property type="entry name" value="metallo-dependent_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000089">
        <property type="entry name" value="Amidohydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="2-AMINO-3-CARBOXYLMUCONATE-6-SEMIALDEHYDE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240:SF19">
        <property type="entry name" value="CATALYTIC_ HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="2">
        <location>
            <begin position="90"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="270" mass="31527" checksum="93A1086B67A444C2" modified="2001-10-01" version="1">MMIKVIDAHVHYHIYARKIPEHCKEFLENVEGTRMTLKNDNVEIEKILLVPSHPCHSEDCYDGFYIDYEERKRNPDLYMQWGEVNPLKCDVKRELERQYSLGIIGIKLHPVHHTFKPNAYREEEGGLKQLLYIYEFAEDHGLPILIHTGTSIGVGSRNKYADPIYADDIAKDFPKLRIILAHAGRPLWYNTAFHMARFTQNIFLEISSIPPKNILKVLPRIHEISDKVIYGSDFPAFKGQDLAEYAYQVYNVLKNEKIMRDNAKRILKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-01-24" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YX1</accession>
    <name>Q97YX1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO7351</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90271">
        <property type="entry name" value="F90271"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YX1"/>
    <dbReference type="PaxDb" id="273057-SSO7351"/>
    <dbReference type="EnsemblBacteria" id="AAK41429">
        <property type="protein sequence ID" value="AAK41429"/>
        <property type="gene ID" value="SSO7351"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7351"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1177"/>
    <dbReference type="eggNOG" id="arCOG09924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2519932_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YX1"/>
    <dbReference type="PhylomeDB" id="Q97YX1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="84" mass="9540" checksum="A6EA8A9DD097E2D2" modified="2001-10-01" version="1">MQTIQVSKTELKSLAITLATININVISQDLDPEIVKAAPSLLTGNRGKYYLKVVRQGEKVISKGQRTFKFYPIYREVKRETNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YZ8</accession>
    <name>Q97YZ8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase U62 modulator of DNA gyrase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1151</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90268">
        <property type="entry name" value="C90268"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923215.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YZ8"/>
    <dbReference type="STRING" id="273057.SSO1151"/>
    <dbReference type="PaxDb" id="273057-SSO1151"/>
    <dbReference type="EnsemblBacteria" id="AAK41402">
        <property type="protein sequence ID" value="AAK41402"/>
        <property type="gene ID" value="SSO1151"/>
    </dbReference>
    <dbReference type="GeneID" id="7806688"/>
    <dbReference type="KEGG" id="sso:SSO1151"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1145"/>
    <dbReference type="eggNOG" id="arCOG00321">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_1_2_2"/>
    <dbReference type="InParanoid" id="Q97YZ8"/>
    <dbReference type="PhylomeDB" id="Q97YZ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="entry name" value="PmbA/TldD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051463">
        <property type="entry name" value="Peptidase_U62_metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN TLDD AND PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624:SF11">
        <property type="entry name" value="ZINC-DEPENDENT PROTEASE, TLDD_PMBA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E N-terminal" evidence="5">
        <location>
            <begin position="16"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E central" evidence="7">
        <location>
            <begin position="100"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="6">
        <location>
            <begin position="216"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="458" mass="51710" checksum="B05996F824EF1B6A" modified="2001-10-01" version="1">MTDLQSLYDKFRYPYVEVRRHIVSTFGMSFLNGRLMGSFRFTDDGYSVRYYKDGVLYFASSNNPNDLLSSNKEYKIEGWESGLDNTEPKEGSYEVKEGKPFDLMSVDEKISFFKNLYEAVKDFRSVKSVSFFYDESIEEKEIIVNGSRIVGRVPRLYVGMNLVLNENNRTATAFYELGGSGGLEVLERMNLNEVVVDKVRSVTEVLAKGKSFGERTTDVVLSNMLTGIMAHESVGHPFEADRVLGREFAQAGLSYLAELKDNRIGSDVVTVIDDPTIPNSGGYYTTDDEGVEARPKYLIKDGKVNEYLQNRFSSSKFNVKSNGSARAANFDREPLIRMSNTFFKPGNMTFDELLEDVKEGVYIKSYMEWNIDDMRLGQRYVGLEAYEIKNGKIGDPVLFPVFQGKTTELLKAVDAVDNTLVFYPGTCGKGDPDQGIPVWLGGPNMRLRNVRIKVMGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z64</accession>
    <name>Q97Z64_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90259">
        <property type="entry name" value="D90259"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z64"/>
    <dbReference type="PaxDb" id="273057-SSO1068"/>
    <dbReference type="EnsemblBacteria" id="AAK41331">
        <property type="protein sequence ID" value="AAK41331"/>
        <property type="gene ID" value="SSO1068"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1068"/>
    <dbReference type="HOGENOM" id="CLU_2313922_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z64"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="99" mass="11455" checksum="F61EC78D87EC0A4B" modified="2001-10-01" version="1">MFYRSLIIFFHFFSSRFGINSCVITRISISLAIIISHKYGKGAKEHNSLALPKKLMIQIMSSGLGKIPLLTLYLIIGRVNFLWQILRIMLKVILRKLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZA1</accession>
    <name>Q97ZA1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Formate dehydrogenase Alpha subunit (FdhF-1)</fullName>
            <ecNumber evidence="10">1.2.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">fdhF-1</name>
        <name evidence="10" type="ordered locus">SSO1029</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.2" evidence="10"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90254">
        <property type="entry name" value="E90254"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989210.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZA1"/>
    <dbReference type="FunCoup" id="Q97ZA1">
        <property type="interactions" value="319"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1029"/>
    <dbReference type="PaxDb" id="273057-SSO1029"/>
    <dbReference type="EnsemblBacteria" id="AAK41292">
        <property type="protein sequence ID" value="AAK41292"/>
        <property type="gene ID" value="SSO1029"/>
    </dbReference>
    <dbReference type="GeneID" id="1454071"/>
    <dbReference type="KEGG" id="sso:SSO1029"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1021"/>
    <dbReference type="eggNOG" id="arCOG01491">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_4_0_2"/>
    <dbReference type="InParanoid" id="Q97ZA1"/>
    <dbReference type="PhylomeDB" id="Q97ZA1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008863">
        <property type="term" value="F:formate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015942">
        <property type="term" value="P:formate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00508">
        <property type="entry name" value="MopB_CT_Fdh-Nap-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02753">
        <property type="entry name" value="MopB_Formate-Dh-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.90">
        <property type="entry name" value="ADC-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.228.10">
        <property type="entry name" value="Dimethylsulfoxide Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041924">
        <property type="entry name" value="Formate_Dh-H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006478">
        <property type="entry name" value="Formate_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01591">
        <property type="entry name" value="Fdh-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43598:SF5">
        <property type="entry name" value="DMSO REDUCTASE CHAIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43598">
        <property type="entry name" value="TUNGSTEN-CONTAINING FORMYLMETHANOFURAN DEHYDROGENASE 2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00932">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="9">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK41292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="657" mass="73613" checksum="6D69E5EC9EC4640E" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZA8</accession>
    <name>Q97ZA8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Formate hydrogenlyase subunit 7 (HycG)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hycG</name>
        <name evidence="8" type="ordered locus">SSO1022</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90253">
        <property type="entry name" value="F90253"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989219.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZA8"/>
    <dbReference type="SMR" id="Q97ZA8"/>
    <dbReference type="STRING" id="273057.SSO1022"/>
    <dbReference type="PaxDb" id="273057-SSO1022"/>
    <dbReference type="EnsemblBacteria" id="AAK41285">
        <property type="protein sequence ID" value="AAK41285"/>
        <property type="gene ID" value="SSO1022"/>
    </dbReference>
    <dbReference type="GeneID" id="84058584"/>
    <dbReference type="KEGG" id="sso:SSO1022"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1013"/>
    <dbReference type="eggNOG" id="arCOG01553">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055737_5_1_2"/>
    <dbReference type="InParanoid" id="Q97ZA8"/>
    <dbReference type="PhylomeDB" id="Q97ZA8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009326">
        <property type="term" value="C:formate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019645">
        <property type="term" value="P:anaerobic electron transport chain"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015944">
        <property type="term" value="P:formate oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052375">
        <property type="entry name" value="Complex_I_20kDa-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42989:SF1">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42989">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="7">
        <location>
            <begin position="95"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="201" mass="22752" checksum="E07FF084223EC5DE" modified="2001-10-01" version="1">MKNWWFIRGLRKGVMTEKYPKEIAEWSTEIQGEGTVNCPTNAIKDGKWIKERCVFCRRCYPNYRPNLNPRIYTINKTQPLFKRSFYLYPIDSGTCGGCNMELKLISSPEYDMTRFSIFFTNTPRHADALVVMGVMTEKMKEVLKKAYEAMPEPKVVILLGACAISGGIIGEGVPLEAVVEIPGCPPNPFTILEALMKVKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZD6</accession>
    <name>Q97ZD6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1955 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0983</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90250">
        <property type="entry name" value="A90250"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZD6"/>
    <dbReference type="STRING" id="273057.SSO0983"/>
    <dbReference type="PaxDb" id="273057-SSO0983"/>
    <dbReference type="EnsemblBacteria" id="AAK41256">
        <property type="protein sequence ID" value="AAK41256"/>
        <property type="gene ID" value="SSO0983"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0983"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.981"/>
    <dbReference type="eggNOG" id="arCOG05998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1574956_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZD6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015288">
        <property type="entry name" value="DUF1955"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036321">
        <property type="entry name" value="ST1625"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09205">
        <property type="entry name" value="DUF1955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101424">
        <property type="entry name" value="Hypothetical protein ST1625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19392" checksum="45CBB0FAC58CE0C6" modified="2001-10-01" version="1">MMSESQELRRKLIEAKRLILDGFVEQGIELLSKTISPENIKESNWIICNIIDTANCDAVVKTLDSIGKIFDISPCANIKRVVYCYALMNKMSEYVDLALDVIVKANKKDSLDKLYNDLKNEKINPEFLLKIGTAYKKLNAVRESNEVLRKACENGVKEACENIKEIASKIM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2025-06-18" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZG1</accession>
    <name>GATA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="1">Glu-ADT subunit A</shortName>
            <ecNumber evidence="1">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatA-2</name>
        <name type="ordered locus">SSO0957</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90246">
        <property type="entry name" value="H90246"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZG1"/>
    <dbReference type="SMR" id="Q97ZG1"/>
    <dbReference type="FunCoup" id="Q97ZG1">
        <property type="interactions" value="139"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0957"/>
    <dbReference type="PaxDb" id="273057-SSO0957"/>
    <dbReference type="EnsemblBacteria" id="AAK41231">
        <property type="protein sequence ID" value="AAK41231"/>
        <property type="gene ID" value="SSO0957"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0957"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.953"/>
    <dbReference type="eggNOG" id="arCOG01717">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_3_2"/>
    <dbReference type="InParanoid" id="Q97ZG1"/>
    <dbReference type="PhylomeDB" id="Q97ZG1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF7">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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        <citation type="journal article" date="2009" name="Proc. Natl. Acad. Sci. U.S.A." volume="106" first="13808" last="13813">
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                <person name="Ye K."/>
                <person name="Jia R."/>
                <person name="Lin J."/>
                <person name="Ju M."/>
                <person name="Peng J."/>
                <person name="Xu A."/>
                <person name="Zhang L."/>
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    <reference evidence="9" key="3">
        <citation type="journal article" date="2011" name="Nature" volume="469" first="559" last="563">
            <title>Structural basis for site-specific ribose methylation by box C/D RNA protein complexes.</title>
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                <person name="Lin J."/>
                <person name="Lai S."/>
                <person name="Jia R."/>
                <person name="Xu A."/>
                <person name="Zhang L."/>
                <person name="Lu J."/>
                <person name="Ye K."/>
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        <citation type="journal article" date="2016" name="Proc. Natl. Acad. Sci. U.S.A." volume="113" first="10878" last="10883">
            <title>Box C/D guide RNAs recognize a maximum of 10 nt of substrates.</title>
            <authorList>
                <person name="Yang Z."/>
                <person name="Lin J."/>
                <person name="Ye K."/>
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    <reference evidence="13" key="5">
        <citation type="journal article" date="2022" name="RNA" volume="28" first="1597" last="1605">
            <title>Methylation guide RNAs without box C/D motifs.</title>
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                <person name="Wang J."/>
                <person name="Yang Z."/>
                <person name="Ye K."/>
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</entry>
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    <accession>Q97ZJ7</accession>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
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                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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    <reference evidence="11" key="2">
        <citation type="journal article" date="2007" name="Nature" volume="449" first="735" last="739">
            <title>Structural basis for selective recognition of ESCRT-III by the AAA ATPase Vps4.</title>
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                <person name="Saksena S."/>
                <person name="Ghazi-Tabatabai S."/>
                <person name="Gill D.J."/>
                <person name="Perisic O."/>
                <person name="Emr S.D."/>
                <person name="Williams R.L."/>
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    <reference evidence="12" key="3">
        <citation type="journal article" date="2014" name="J. Mol. Biol." volume="426" first="510" last="525">
            <title>The oligomeric state of the active Vps4 AAA ATPase.</title>
            <authorList>
                <person name="Monroe N."/>
                <person name="Han H."/>
                <person name="Gonciarz M.D."/>
                <person name="Eckert D.M."/>
                <person name="Karren M.A."/>
                <person name="Whitby F.G."/>
                <person name="Sundquist W.I."/>
                <person name="Hill C.P."/>
            </authorList>
            <dbReference type="PubMed" id="24161953"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2013.09.043"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.71 ANGSTROMS) OF 85-372</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90241">
        <property type="entry name" value="H90241"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992337.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2V6Y">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=1-83"/>
    </dbReference>
    <dbReference type="PDB" id="4LGM">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.71 A"/>
        <property type="chains" value="A=85-372"/>
    </dbReference>
    <dbReference type="PDBsum" id="2V6Y"/>
    <dbReference type="PDBsum" id="4LGM"/>
    <dbReference type="AlphaFoldDB" id="Q97ZJ7"/>
    <dbReference type="SMR" id="Q97ZJ7"/>
    <dbReference type="DIP" id="DIP-60244N"/>
    <dbReference type="FunCoup" id="Q97ZJ7">
        <property type="interactions" value="199"/>
    </dbReference>
    <dbReference type="IntAct" id="Q97ZJ7">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0909"/>
    <dbReference type="PaxDb" id="273057-SSO0909"/>
    <dbReference type="EnsemblBacteria" id="AAK41191">
        <property type="protein sequence ID" value="AAK41191"/>
        <property type="gene ID" value="SSO0909"/>
    </dbReference>
    <dbReference type="GeneID" id="1455158"/>
    <dbReference type="KEGG" id="sso:SSO0909"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.911"/>
    <dbReference type="eggNOG" id="arCOG01307">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_21_2_2"/>
    <dbReference type="InParanoid" id="Q97ZJ7"/>
    <dbReference type="PhylomeDB" id="Q97ZJ7"/>
    <dbReference type="BRENDA" id="3.6.1.15">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="3.6.4.6">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97ZJ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02682">
        <property type="entry name" value="MIT_AAA_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19509">
        <property type="entry name" value="RecA-like_VPS4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000313">
        <property type="entry name" value="AAA ATPase, central domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001054">
        <property type="entry name" value="ATPase, AAA family, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054951">
        <property type="entry name" value="cell_div_CdvC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041006">
        <property type="entry name" value="cell_div_CdvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF83">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="11 12" id="KW-0002">3D-structure</keyword>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIT" evidence="8">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="133"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="11">
        <source>
            <dbReference type="PDB" id="2V6Y"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="12">
        <source>
            <dbReference type="PDB" id="4LGM"/>
        </source>
    </evidence>
    <sequence length="372" mass="42248" checksum="D17D6849C9A1B696" modified="2001-10-01" version="1">MSAQVMLEDMARKYAILAVKADKEGKVEDAITYYKKAIEVLSQIIVLYPESVARTAYEQMINEYKKRISYLEKVLPASSDGSGDNTSPPEEVVITEKPKVSFKDIVGLDDVKEALREAIIYPTKRPDLFPLGWPRGILLYGPPGCGKTMIAAAVANEIDSIFMQLDAASVMSKWLGEAEKNVANVFKMAREESKKQNKPAIIFIDELDALLGVYSTEVGGEARVRNQFLKEMDGLLDKSENYKVYVIGATNKPWRLDEAFLRRFQKRIYVPLPDYEQRLSLFKYYTSKIKLDTEVSLEELAKLTEGYTASDIRDIVQAAHIKVVKEMFKNNLGEPRTITLQDFKDILKVRMPSVNPELIKAYEAWTEKFKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZK1</accession>
    <name>Q97ZK1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">D-3-phosphoglycerate dehydrogenase (SerA-1)</fullName>
            <ecNumber evidence="8">1.1.1.95</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">serA-1</name>
        <name evidence="8" type="ordered locus">SSO0905</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.95" evidence="8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90241">
        <property type="entry name" value="D90241"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZK1"/>
    <dbReference type="FunCoup" id="Q97ZK1">
        <property type="interactions" value="315"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0905"/>
    <dbReference type="PaxDb" id="273057-SSO0905"/>
    <dbReference type="EnsemblBacteria" id="AAK41187">
        <property type="protein sequence ID" value="AAK41187"/>
        <property type="gene ID" value="SSO0905"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0905"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.907"/>
    <dbReference type="eggNOG" id="arCOG01754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_3_2"/>
    <dbReference type="InParanoid" id="Q97ZK1"/>
    <dbReference type="PhylomeDB" id="Q97ZK1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047545">
        <property type="term" value="F:(S)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05303">
        <property type="entry name" value="PGDH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000041">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050857">
        <property type="entry name" value="D-2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789:SF1">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="6">
        <location>
            <begin position="20"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="7">
        <location>
            <begin position="122"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="326" mass="36220" checksum="4D6D21491DCD9B3C" modified="2001-10-01" version="1">MISMETIQGVNSIGKLDFKVLITDPVDQYMIRTLQNNGLIVDYKPEITREELLKIIDQYQVLIVRSRTKVDKEIIRYGVNLKIIARAGIGLDNIDTEEASKRNIKIVYAPGASTDSAAELTIGLLIAAARKLYDSMNMAKGGIFKKIEGIELAGKTIGIVGFGRIGTKVAKVCKALDMNVIAYDVINIKEKANIVGVRVAESLEELLKNSDVITFHVTVGKDAKPILNKDTFNYIKDNAIIINTSRAVVIDGKALLEYIDKKHLTYATDVLWNEPPKEDWEIKLLRHERVIVTTHIGAQTKEAQHRVAVVTTDNLITALKEIGVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZS6</accession>
    <name>Q97ZS6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Urease accessory protein UreH-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90195">
        <property type="entry name" value="G90195"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991396.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZS6"/>
    <dbReference type="STRING" id="273057.SSO0503"/>
    <dbReference type="PaxDb" id="273057-SSO0503"/>
    <dbReference type="EnsemblBacteria" id="AAK40822">
        <property type="protein sequence ID" value="AAK40822"/>
        <property type="gene ID" value="SSO0503"/>
    </dbReference>
    <dbReference type="GeneID" id="1455632"/>
    <dbReference type="KEGG" id="sso:SSO0503"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.497"/>
    <dbReference type="eggNOG" id="arCOG06008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_942587_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZS6"/>
    <dbReference type="PhylomeDB" id="Q97ZS6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="32896" checksum="D16F937FA6961F1C" modified="2001-10-01" version="1">MISISTLLNPPEGLTISEILLISVILGMLHGATPDEHTWPITFSYAIGKYSSKGGMKAGFLFSLGFTIQRAFLTTIGFIGLAAIYEKYNLDGPVYTIVGIVMAIAGSYILKGRYLHLPIDVLFKSKYHNTKTTGAELHDVPLKMTIVHGLIAGFGFGAYASIITFVLAPQLPSIVYAPLPGLMFGIGTMIMQIIFGAIFGNILRAKKLTEEKISYVARKTAGRTLYYGGLVFTIVGLLIIGFPIIDNFAISTGNPIPNLDAIDIGFLLVISVVGIIGISSIILGIKEATRLSGTNQKPGSLSGYKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZU0</accession>
    <name>Q97ZU0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Iron (III) ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0487</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99194">
        <property type="entry name" value="A99194"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923002.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZU0"/>
    <dbReference type="STRING" id="273057.SSO0487"/>
    <dbReference type="PaxDb" id="273057-SSO0487"/>
    <dbReference type="EnsemblBacteria" id="AAK40808">
        <property type="protein sequence ID" value="AAK40808"/>
        <property type="gene ID" value="SSO0487"/>
    </dbReference>
    <dbReference type="GeneID" id="15298019"/>
    <dbReference type="KEGG" id="sso:SSO0487"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.481"/>
    <dbReference type="eggNOG" id="arCOG00198">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_2"/>
    <dbReference type="InParanoid" id="Q97ZU0"/>
    <dbReference type="PhylomeDB" id="Q97ZU0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43850">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN MA_4021-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43850:SF2">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN MA_4021-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="2"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="219" mass="24407" checksum="3B502CA8B8886243" modified="2001-10-01" version="1">MLTLRNISVRLGGKLILSSISFSAVKGINVILGPNGSGKTTLLRAIIGMIKYDGEIRVDGEISYVPAEFFSPSMKVLDVLKAGRKRANYESYIKELNVEKFLERDFSTLSSGERKLILVTKALAEGDNVIMDEPLSNLDVKNKFFVMNILKKFNHKTFLITSHELETLRYADKAIVINKGKLEYDGGVSDLSDDLLSNVYGIKFKKIQVDGKTFFNVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZZ1</accession>
    <name>Q97ZZ1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90187">
        <property type="entry name" value="D90187"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZZ1"/>
    <dbReference type="FunCoup" id="Q97ZZ1">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0430"/>
    <dbReference type="PaxDb" id="273057-SSO0430"/>
    <dbReference type="EnsemblBacteria" id="AAK40755">
        <property type="protein sequence ID" value="AAK40755"/>
        <property type="gene ID" value="SSO0430"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0430"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.423"/>
    <dbReference type="eggNOG" id="arCOG01617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_3_2"/>
    <dbReference type="InParanoid" id="Q97ZZ1"/>
    <dbReference type="PhylomeDB" id="Q97ZZ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd19086">
        <property type="entry name" value="AKR_AKR11C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053135">
        <property type="entry name" value="AKR2_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43312">
        <property type="entry name" value="D-THREO-ALDOSE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43312:SF1">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="43"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="354" mass="41550" checksum="B41A2C1916D76C7D" modified="2001-10-01" version="1">MLFVYLNSHNYHLYFYYLKCNDNNGRYYMLYRRIGDTNITVSEIGIGVWSLVTDWWGADTNKAEDIIRRAYEVGINFYDTADTYGEGKGEEIIAKVLETKRDNIVILTKIGYDFYHKEGGKIRQRFDIPYLEFALKRSLERLRTDYIDILMIHNPKMNAIKNLEILSFLRSLKKDGIIRMYGVALGPTLGWEEEGIEAINMGYESLEYIYNIIEQRPGNKFLKYEKVGHIIRVPHASDVLNEDKWPLISDPKLHRSLKDIKWLNEGIKRSLKLKEFASSKGMTLSDLALKFILSNKNVSTVIPNITSINELEKYVKVENLSILTEDDLKYIGEYYRKYYEDLNEDSIKETLRYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q980H7</accession>
    <name>Q980H7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90174">
        <property type="entry name" value="H90174"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980H7"/>
    <dbReference type="PaxDb" id="273057-SSO0320"/>
    <dbReference type="EnsemblBacteria" id="AAK40655">
        <property type="protein sequence ID" value="AAK40655"/>
        <property type="gene ID" value="SSO0320"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0320"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.313"/>
    <dbReference type="eggNOG" id="arCOG06038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_712935_0_0_2"/>
    <dbReference type="InParanoid" id="Q980H7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="392" mass="44603" checksum="F7E9CBDDA820C556" modified="2001-10-01" version="1">MILNSRTIDDAKFFDKPILLFFTDENCKDCDTIYSKLQDSTIFSKYIGKKVNALEYIHYTIRLTRGMIPTITILSPTLDLLAVIESKDLKFIENKLRELLDEYKRRIVKGIKLEGYTPDPIEPTPSIIYETINKVIGGYEADTRMIEVYLTYTNVYKEYLKAKDKIKCSDDVARYLLEGKGEIKIDEKSSANVSMLVNYGVTSVQKLLDFIDINSGEVYRSKKKETKGILLDEALAGNALVSEYERTINEEYLSLAIKVADYIKNNLQHERGFRDIKEIDNITKVPYLEPISNSEASIFFSRLWNITGDETYKNLALKGLGSALGGALNNPKVIARAAIAYLKLFDSIKANRYLNIIDARITVVKNNGCNDSMLYYSGQCYSKIDEIQPTAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q980R7</accession>
    <name>Q980R7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90162">
        <property type="entry name" value="E90162"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990462.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980R7"/>
    <dbReference type="STRING" id="273057.SSO0213"/>
    <dbReference type="PaxDb" id="273057-SSO0213"/>
    <dbReference type="EnsemblBacteria" id="AAK40556">
        <property type="protein sequence ID" value="AAK40556"/>
        <property type="gene ID" value="SSO0213"/>
    </dbReference>
    <dbReference type="GeneID" id="1455366"/>
    <dbReference type="KEGG" id="sso:SSO0213"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.211"/>
    <dbReference type="eggNOG" id="arCOG01831">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_914055_0_0_2"/>
    <dbReference type="InParanoid" id="Q980R7"/>
    <dbReference type="PhylomeDB" id="Q980R7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="113"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="299" mass="34591" checksum="CCB2404F07606F4B" modified="2001-10-01" version="1">MGRDYFLDRDIIIDKEGNVYTVLTNYNPPGYVFAYLKYVYTGKGIWNGYERIFKKYGVHNLIKINQRFNFESCYDASFPIVLLSNISRHLKPEERLREILKKSVSDSIIYALISFIEEYVRVNDIGVTGSILLGNYHSNSDIDIIIYGRKSALDFIESFDGFEKDSEWIIEANENYGINFSSLLYDNRRRGIYRGIKISVLFADDKPWRYCNDICKKIGKVKFKAHISGDYEALIYPSVAYVLDNNEYNVSKIVSYEGIFSSALFGKRQVEVEGLLMSCEKENVVIIGDKDVRGFIRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q980X6</accession>
    <name>Q980X6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carbohydrate kinase PfkB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99155">
        <property type="entry name" value="A99155"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980X6"/>
    <dbReference type="STRING" id="273057.SSO0149"/>
    <dbReference type="PaxDb" id="273057-SSO0149"/>
    <dbReference type="EnsemblBacteria" id="AAK40496">
        <property type="protein sequence ID" value="AAK40496"/>
        <property type="gene ID" value="SSO0149"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0149"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.146"/>
    <dbReference type="eggNOG" id="arCOG00016">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1052188_0_0_2"/>
    <dbReference type="InParanoid" id="Q980X6"/>
    <dbReference type="PhylomeDB" id="Q980X6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="29631" checksum="6CDA514AE180E7EB" modified="2001-10-01" version="1">MVKKKVKRSKILIVGGFTIDEIQGKIRPGGPLIYSSLGVMRGGGIPKLYGLIGRDFDFEIDFISDLEKQLIYDEYTIRFKIDLTNEGRKLILLKKPKNRIKVISDNTVDGILVNPVCKEVDNMSIQSPIPIAVDIQGFVRNCIEYEEIRYEMGFRFPFNSNYMVMHGNREELESSKLELSDLFKVGFKEIIVSDGHNGFNVYTNMLAKYTYRPSKIGKNEIGTGDFLLGAYFALRLGGLEIIEAATIAGKLTEEFSNSEFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Y3</accession>
    <name>Q980Y3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ORF D-335-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90154">
        <property type="entry name" value="B90154"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Y3"/>
    <dbReference type="PaxDb" id="273057-SSO0138"/>
    <dbReference type="EnsemblBacteria" id="AAK40489">
        <property type="protein sequence ID" value="AAK40489"/>
        <property type="gene ID" value="SSO0138"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0138"/>
    <dbReference type="eggNOG" id="arCOG07277">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1998840_0_0_2"/>
    <dbReference type="InParanoid" id="Q980Y3"/>
    <dbReference type="PhylomeDB" id="Q980Y3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012922">
        <property type="entry name" value="ORF_D-335"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07935">
        <property type="entry name" value="SSV1_ORF_D-335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ORF D-335-like" evidence="2">
        <location>
            <begin position="41"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="124" mass="14002" checksum="756DC243CF843505" modified="2001-10-01" version="1">MILWQKLRSCQSLKRNPENHNTGEKVNGKKSGRSMGLTRAFKCENIILRERKGRYYVYKLETINGEVKETYVGPLADVVETYEKLKDSSGGVGVSPTWARWDLNPGSSPCEGDVLTKLDDGPFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q981A0</accession>
    <name>Q981A0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator PadR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90144">
        <property type="entry name" value="G90144"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988848.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981A0"/>
    <dbReference type="STRING" id="273057.SSO0049"/>
    <dbReference type="PaxDb" id="273057-SSO0049"/>
    <dbReference type="EnsemblBacteria" id="AAK40414">
        <property type="protein sequence ID" value="AAK40414"/>
        <property type="gene ID" value="SSO0049"/>
    </dbReference>
    <dbReference type="GeneID" id="1455305"/>
    <dbReference type="KEGG" id="sso:SSO0049"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.52"/>
    <dbReference type="eggNOG" id="arCOG00002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_1_3_2"/>
    <dbReference type="InParanoid" id="Q981A0"/>
    <dbReference type="PhylomeDB" id="Q981A0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR YQJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, PADR-LIKE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="13"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="131" mass="15331" checksum="87F03419AE25C5B6" modified="2001-10-01" version="1">MFWHRRRGLKWLILYVLSKGPMTGAQIMDEIEKTSHGMWRPSPGSIYPALDALESEGLIRISRVDGWKKYYELTEEGRKAIGAMSEEDKIKEAIEQLEFSARYIVENLEKLNDEDKRKVKNILDELSKVMR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UWV6</accession>
    <name>TSR3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">16S rRNA aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="2">2.5.1.157</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">20S S rRNA accumulation protein 3 homolog</fullName>
            <shortName evidence="5">SsTsr3</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0551</name>
        <name type="ORF">C21_048</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="journal article" date="2016" name="Nucleic Acids Res." volume="44" first="4304" last="4316">
            <title>Ribosome biogenesis factor Tsr3 is the aminocarboxypropyl transferase responsible for 18S rRNA hypermodification in yeast and humans.</title>
            <authorList>
                <person name="Meyer B."/>
                <person name="Wurm J.P."/>
                <person name="Sharma S."/>
                <person name="Immer C."/>
                <person name="Pogoryelov D."/>
                <person name="Kotter P."/>
                <person name="Lafontaine D.L."/>
                <person name="Wohnert J."/>
                <person name="Entian K.D."/>
            </authorList>
            <dbReference type="PubMed" id="27084949"/>
            <dbReference type="DOI" id="10.1093/NAR/GKW244"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS)</scope>
        <scope>S-ADENOSYL-L-METHIONINE-BINDING</scope>
        <scope>RRNA-BINDING</scope>
        <scope>MUTAGENESIS OF ASP-63 AND TRP-66</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi).</text>
    </comment>
    <comment type="function">
        <text evidence="4">Fully modified m1acp3-Psi 930 is seen in 16S rRNA in ribosomal 30S initiation complexes (PubMed:39753558).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine in rRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63296"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:16310"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
            <dbReference type="EC" id="2.5.1.157"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDD superfamily. TSR3 family.</text>
    </comment>
    <comment type="sequence caution" evidence="6">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40868" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <comment type="sequence caution" evidence="6">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAB57748" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.157" evidence="2"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57748.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40868.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90201">
        <property type="entry name" value="E90201"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991056.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="5AP8">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.25 A"/>
        <property type="chains" value="A/B/C=1-166"/>
    </dbReference>
    <dbReference type="PDBsum" id="5AP8"/>
    <dbReference type="AlphaFoldDB" id="Q9UWV6"/>
    <dbReference type="SMR" id="Q9UWV6"/>
    <dbReference type="FunCoup" id="Q9UWV6">
        <property type="interactions" value="131"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0551"/>
    <dbReference type="PaxDb" id="273057-SSO0551"/>
    <dbReference type="EnsemblBacteria" id="AAK40868">
        <property type="protein sequence ID" value="AAK40868"/>
        <property type="gene ID" value="SSO0551"/>
    </dbReference>
    <dbReference type="GeneID" id="1454835"/>
    <dbReference type="KEGG" id="sso:SSO0551"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.560"/>
    <dbReference type="eggNOG" id="arCOG04733">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035060_4_1_2"/>
    <dbReference type="InParanoid" id="Q9UWV6"/>
    <dbReference type="PhylomeDB" id="Q9UWV6"/>
    <dbReference type="EvolutionaryTrace" id="Q9UWV6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106388">
        <property type="term" value="F:rRNA small subunit aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01116">
        <property type="entry name" value="TSR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002621">
        <property type="entry name" value="PRK02287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426:SF0">
        <property type="entry name" value="18S RRNA AMINOCARBOXYPROPYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN TSR3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04034">
        <property type="entry name" value="Ribo_biogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000094422" description="16S rRNA aminocarboxypropyltransferase">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Does not affect S-adenosyl-L-methionine-binding." evidence="3">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Decreased S-adenosyl-L-methionine-binding." evidence="3">
        <original>W</original>
        <variation>A</variation>
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="3"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="19"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="26"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="35"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="43"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="51"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="59"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="66"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="71"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="74"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="78"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="92"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="117"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="123"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="130"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="138"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="161"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="E1QU22"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="27084949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="27084949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="5AP8"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="5AP8"/>
        </source>
    </evidence>
    <sequence length="166" mass="19197" checksum="41B644E0B04AECFB" modified="2003-11-28" version="2">MKVYIIDYHKDDPKRCTGKKLVKLKIAEFTRVGKGVVLDPFAQITLSNKDKDIVRRIGITIVDTSWNNTSQSEFKNIRGEHRRIPILFAGNPIHYGIAYKLSSIEALIATLYIVDEVEEAIKLSNVVKWGHTFIELNKELLEAYKNKTEEDIKKIEREIIEKILEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXF6</accession>
    <name>RFCL_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Replication factor C large subunit</fullName>
            <shortName>RFC large subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Clamp loader large subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>SsoRFC large subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rfcL</name>
        <name type="ordered locus">SSO0769</name>
        <name type="ORF">C40_005</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2000" name="J. Mol. Biol." volume="301" first="61" last="73">
            <title>Biochemical characterization of a clamp-loader complex homologous to eukaryotic replication factor C from the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Pisani F.M."/>
                <person name="De Felice M."/>
                <person name="Carpentieri F."/>
                <person name="Rossi M."/>
            </authorList>
            <dbReference type="PubMed" id="10926493"/>
            <dbReference type="DOI" id="10.1006/jmbi.2000.3964"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2003" name="Mol. Cell" volume="11" first="275" last="282">
            <title>A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Dionne I."/>
                <person name="Nookala R.K."/>
                <person name="Jackson S.P."/>
                <person name="Doherty A.J."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="12535540"/>
            <dbReference type="DOI" id="10.1016/S1097-2765(02)00824-9"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH PCNA3</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-dependent ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3">Heteropentamer composed of four small subunits (RfcS) and one large subunit (RfcL). Probably interacts with PCNA subunit PCNA3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the activator 1 small subunits family. RfcL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90226">
        <property type="entry name" value="C90226"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991358.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXF6"/>
    <dbReference type="SMR" id="Q9UXF6"/>
    <dbReference type="STRING" id="273057.SSO0769"/>
    <dbReference type="PaxDb" id="273057-SSO0769"/>
    <dbReference type="EnsemblBacteria" id="AAK41066">
        <property type="protein sequence ID" value="AAK41066"/>
        <property type="gene ID" value="SSO0769"/>
    </dbReference>
    <dbReference type="GeneID" id="1455033"/>
    <dbReference type="KEGG" id="sso:SSO0769"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.768"/>
    <dbReference type="eggNOG" id="arCOG00470">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027255_1_1_2"/>
    <dbReference type="InParanoid" id="Q9UXF6"/>
    <dbReference type="PhylomeDB" id="Q9UXF6"/>
    <dbReference type="BRENDA" id="3.6.4.B8">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01508">
        <property type="entry name" value="RfcL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023935">
        <property type="entry name" value="Rep_factor-C_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003226">
        <property type="entry name" value="PRK04195.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003229">
        <property type="entry name" value="PRK04195.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF6">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135966" description="Replication factor C large subunit">
        <location>
            <begin position="1"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="47"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10926493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12535540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="405" mass="46787" checksum="D9CF8AF297999E46" modified="2000-05-01" version="1">MIQWFLKYRPRSLKDVENQDDAKKQLQEWIESWLNGNSNVKAVLLHGPPGVGKTVLAEALAHDYNFELLEMNASDSRKLQDIKSIAEKAAVYGSIFGTKGKLILLDEVDGINVREDTGAIQGILELIEKTKYPIIMTANDPWNPGLRELRNKAKMIELSKLGKYPLRRILKKICQAEKIICDDEALNYIIDSSEGDARYAINILQGIGEGYGKVTLNLVESLAKRKERELDPFETLRDIFWARYAWQAKNAATSAQIDYDMLIRWISENIPIQYDNIEDIWRAFDALSRASIFLKRAKSGDWDLLSYAYDMMSSGVAFAEPEKKKPNWKPKWKKYQFPSYIQLLSKSKDIRDTRDEIIKKLAIHSSFNKALNDTYPFFLLFYKKYDKHLNLNTKEKEYLSSISKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="149" xmlns="https://uniprot.org/uniprot">
    <accession>O33780</accession>
    <accession>Q9UWZ6</accession>
    <name>SYS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Seryl-tRNA synthetase</fullName>
            <shortName evidence="1">SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">serS</name>
        <name type="ordered locus">SSO0602</name>
        <name type="ORF">C08_048</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1997" name="J. Bacteriol." volume="179" first="4429" last="4432">
            <title>Evolutionary analysis of the hisCGABdFDEHI gene cluster from the archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Sensen C.W."/>
                <person name="Doolittle W.F."/>
                <person name="Brown J.R."/>
            </authorList>
            <dbReference type="PubMed" id="9209067"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-100</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Ser) + L-serine + ATP = L-seryl-tRNA(Ser) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Sec) + L-serine + ATP = L-seryl-tRNA(Sec) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. The tRNA molecule binds across the dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="U82227">
        <property type="protein sequence ID" value="AAB63028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90207">
        <property type="entry name" value="C90207"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991125.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O33780"/>
    <dbReference type="SMR" id="O33780"/>
    <dbReference type="FunCoup" id="O33780">
        <property type="interactions" value="297"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0602"/>
    <dbReference type="PaxDb" id="273057-SSO0602"/>
    <dbReference type="EnsemblBacteria" id="AAK40914">
        <property type="protein sequence ID" value="AAK40914"/>
        <property type="gene ID" value="SSO0602"/>
    </dbReference>
    <dbReference type="GeneID" id="1454881"/>
    <dbReference type="GeneID" id="44129604"/>
    <dbReference type="KEGG" id="sso:SSO0602"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.610"/>
    <dbReference type="eggNOG" id="arCOG00403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_0_1_2"/>
    <dbReference type="InParanoid" id="O33780"/>
    <dbReference type="PhylomeDB" id="O33780"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.40:FF:000004">
        <property type="entry name" value="Serine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000048">
        <property type="entry name" value="Serine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00176">
        <property type="entry name" value="Ser_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000122185" description="Serine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="252"/>
            <end position="254"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="283"/>
            <end position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="370"/>
            <end position="373"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00176"/>
        </source>
    </evidence>
    <sequence length="457" mass="53187" checksum="0139956349C90083" modified="2000-12-01" version="2">MSWSILELLRKNPEDLKNNLKRRAIDVSLVDKAVELDKKWRQVLQEVEKLRHQHNVLSSQISKLSGEERKKKIEESKNLLKILEEKEKELEEIENERDRLLSSLPNLVADDVPNGPDDSYNVPIKFWGKFKVYEGDVQEFLKQIKDAKVNYEVIKWKPKGHAEMLEDVLHLGNTLKAAEIAGSRFYYLFNDIVWLDFSLLLFAIDYITQQGYTLVLPPYMLRGEVIQSVIDLDTFKDAIYKIENEDLYLIATAEHPIAAMFFKEEIEKDKLPLKFAGISPAFRKEASAANKDLKGIFRVHQFHKVEQFIFSTPEDSWKYHSELITNAESIFQQLELPYRIVNIASGDLGACAAKKFDLEVWMPAQAKFREMVSCSNCTDWQAFRMKIRYVDRKNNRRGYVHTLNSTAIASTRTITAILENYQREDGVVEVPKVLRKYLEAFPKAPKDYIYPLKNKII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-02-21" modified="2025-06-18" version="138" xmlns="https://uniprot.org/uniprot">
    <accession>P57765</accession>
    <name>PCNA3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA polymerase sliding clamp 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Proliferating cell nuclear antigen homolog 3</fullName>
            <shortName evidence="1">PCNA3</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pcn3</name>
        <name type="synonym">pcnA-1</name>
        <name type="ordered locus">SSO0405</name>
        <name type="ORF">C41_016</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1999" name="J. Mol. Biol." volume="291" first="47" last="57">
            <title>Two DNA polymerase sliding clamps from the thermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="De Felice M."/>
                <person name="Sensen C.W."/>
                <person name="Charlebois R.L."/>
                <person name="Rossi M."/>
                <person name="Pisani F.M."/>
            </authorList>
            <dbReference type="PubMed" id="10438605"/>
            <dbReference type="DOI" id="10.1006/jmbi.1999.2939"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 1-10</scope>
        <scope>CHARACTERIZATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Mol. Cell" volume="11" first="275" last="282">
            <title>A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Dionne I."/>
                <person name="Nookala R.K."/>
                <person name="Jackson S.P."/>
                <person name="Doherty A.J."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="12535540"/>
            <dbReference type="DOI" id="10.1016/S1097-2765(02)00824-9"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH DPO1; LIG AND RFCL</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2003" name="Mol. Microbiol." volume="48" first="361" last="371">
            <title>An archaeal XPF repair endonuclease dependent on a heterotrimeric PCNA.</title>
            <authorList>
                <person name="Roberts J.A."/>
                <person name="Bell S.D."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="12675797"/>
            <dbReference type="DOI" id="10.1046/j.1365-2958.2003.03444.x"/>
        </citation>
        <scope>INTERACTION WITH XPF</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2006" name="J. Mol. Biol." volume="364" first="243" last="247">
            <title>PCNA activates the Holliday junction endonuclease Hjc.</title>
            <authorList>
                <person name="Dorazi R."/>
                <person name="Parker J.L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="17011573"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2006.09.011"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2006" name="Nucleic Acids Res." volume="34" first="4515" last="4526">
            <title>Structure of an archaeal PCNA1-PCNA2-FEN1 complex: elucidating PCNA subunit and client enzyme specificity.</title>
            <authorList>
                <person name="Dore A.S."/>
                <person name="Kilkenny M.L."/>
                <person name="Jones S.A."/>
                <person name="Oliver A.W."/>
                <person name="Roe S.M."/>
                <person name="Bell S.D."/>
                <person name="Pearl L.H."/>
            </authorList>
            <dbReference type="PubMed" id="16945955"/>
            <dbReference type="DOI" id="10.1093/nar/gkl623"/>
        </citation>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2009" name="Mol. Microbiol." volume="71" first="678" last="691">
            <title>Structural insight into recruitment of translesion DNA polymerase Dpo4 to sliding clamp PCNA.</title>
            <authorList>
                <person name="Xing G."/>
                <person name="Kirouac K."/>
                <person name="Shin Y.J."/>
                <person name="Bell S.D."/>
                <person name="Ling H."/>
            </authorList>
            <dbReference type="PubMed" id="19054331"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.2008.06553.x"/>
        </citation>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2006" name="Acta Crystallogr. F Struct. Biol. Commun." volume="62" first="944" last="948">
            <title>Structure of the heterotrimeric PCNA from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Williams G.J."/>
                <person name="Johnson K."/>
                <person name="Rudolf J."/>
                <person name="McMahon S.A."/>
                <person name="Carter L."/>
                <person name="Oke M."/>
                <person name="Liu H."/>
                <person name="Taylor G.L."/>
                <person name="White M.F."/>
                <person name="Naismith J.H."/>
            </authorList>
            <dbReference type="PubMed" id="17012780"/>
            <dbReference type="DOI" id="10.1107/S1744309106034075"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="9">
        <citation type="journal article" date="2006" name="Mol. Cell" volume="24" first="279" last="291">
            <title>A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA.</title>
            <authorList>
                <person name="Pascal J.M."/>
                <person name="Tsodikov O.V."/>
                <person name="Hura G.L."/>
                <person name="Song W."/>
                <person name="Cotner E.A."/>
                <person name="Classen S."/>
                <person name="Tomkinson A.E."/>
                <person name="Tainer J.A."/>
                <person name="Ellenberger T."/>
            </authorList>
            <dbReference type="PubMed" id="17052461"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2006.08.015"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.79 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="10">
        <citation type="journal article" date="2008" name="Acta Crystallogr. D" volume="64" first="941" last="949">
            <title>Structures of monomeric, dimeric and trimeric PCNA: PCNA-ring assembly and opening.</title>
            <authorList>
                <person name="Hlinkova V."/>
                <person name="Xing G."/>
                <person name="Bauer J."/>
                <person name="Shin Y.J."/>
                <person name="Dionne I."/>
                <person name="Rajashankar K.R."/>
                <person name="Bell S.D."/>
                <person name="Ling H."/>
            </authorList>
            <dbReference type="PubMed" id="18703842"/>
            <dbReference type="DOI" id="10.1107/S0907444908021665"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2 5">One of the sliding clamp subunits that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication. DNA polymerase I, DNA ligase and the flap endonuclease may be constitutively associated with the PCNA heterotrimer forming a scanning complex able to couple DNA synthesis and Okazaki fragment maturation. Heterotrimer stimulates the Holliday junction resolvase Hjc.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3 4 5 6 7 8 9">Does not individually interact with either of the other PCNA subunits, but is recruited to a PCNA1-PCNA2 heterodimer; does not form homotrimers (PubMed:12535540, PubMed:18703842). Heterotrimer which circularizes head-to-tail (head is at N-terminus, tail is at C-terminus) to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA. This subunit interacts with DNA ligase, RfcL, XPF, and weakly with DNA polymerase I (PubMed:12535540).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PCNA family.</text>
    </comment>
    <comment type="sequence caution" evidence="10">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40734" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
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        <property type="protein sequence ID" value="AAK40734.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="G90184"/>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.79 A"/>
        <property type="chains" value="C/Z=1-244"/>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.30 A"/>
        <property type="chains" value="C/N/Z=1-244"/>
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    <dbReference type="PDB" id="2IJX">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A/B/C/D=1-244"/>
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    <dbReference type="PDB" id="2IX2">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="C=1-244"/>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="C/F/I=1-244"/>
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    <dbReference type="PDB" id="7RPO">
        <property type="method" value="EM"/>
        <property type="resolution" value="4.16 A"/>
        <property type="chains" value="C=1-244"/>
    </dbReference>
    <dbReference type="PDB" id="7RPW">
        <property type="method" value="EM"/>
        <property type="resolution" value="4.38 A"/>
        <property type="chains" value="C=1-244"/>
    </dbReference>
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        <property type="method" value="EM"/>
        <property type="resolution" value="4.20 A"/>
        <property type="chains" value="C=1-244"/>
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    <dbReference type="PDBsum" id="2HII"/>
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    <dbReference type="PDBsum" id="2IX2"/>
    <dbReference type="PDBsum" id="2NTI"/>
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    <dbReference type="AlphaFoldDB" id="P57765"/>
    <dbReference type="EMDB" id="EMD-24618"/>
    <dbReference type="EMDB" id="EMD-24624"/>
    <dbReference type="EMDB" id="EMD-24625"/>
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    <dbReference type="FunCoup" id="P57765">
        <property type="interactions" value="193"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0405"/>
    <dbReference type="PaxDb" id="273057-SSO0405"/>
    <dbReference type="EnsemblBacteria" id="AAK40734">
        <property type="protein sequence ID" value="AAK40734"/>
        <property type="gene ID" value="SSO0405"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0405"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.401"/>
    <dbReference type="eggNOG" id="arCOG00488">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043978_1_0_2"/>
    <dbReference type="InParanoid" id="P57765"/>
    <dbReference type="PhylomeDB" id="P57765"/>
    <dbReference type="EvolutionaryTrace" id="P57765"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="P:leading strand elongation"/>
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    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.70.10.10:FF:000062">
        <property type="entry name" value="DNA polymerase sliding clamp 3"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
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        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
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        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
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        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
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    <dbReference type="NCBIfam" id="NF002220">
        <property type="entry name" value="PRK01115.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000149214" description="DNA polymerase sliding clamp 3">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="2"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="23"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="31"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
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            <begin position="53"/>
            <end position="55"/>
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    </feature>
    <feature type="strand" evidence="11">
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            <begin position="57"/>
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        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="65"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="71"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="86"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="96"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="129"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="turn" evidence="11">
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            <begin position="147"/>
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        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="151"/>
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    <feature type="strand" evidence="11">
        <location>
            <begin position="161"/>
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        <location>
            <begin position="235"/>
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    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12535540"/>
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            <dbReference type="PubMed" id="17011573"/>
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            <dbReference type="PubMed" id="17012780"/>
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            <dbReference type="PubMed" id="17052461"/>
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            <dbReference type="PubMed" id="18703842"/>
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            <dbReference type="PubMed" id="19054331"/>
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        <source>
            <dbReference type="PDB" id="2IJX"/>
        </source>
    </evidence>
    <sequence length="244" mass="27459" checksum="191C3C1267F1F5D1" modified="2001-02-21" version="1">MKVVYDDVRVLKDIIQALARLVDEAVLKFKQDSVELVALDRAHISLISVNLPREMFKEYDVNDEFKFGFNTQYLMKILKVAKRKEAIEIASESPDSVIINIIGSTNREFNVRNLEVSEQEIPEINLQFDISATISSDGFKSAISEVSTVTDNVVVEGHEDRILIKAEGESEVEVEFSKDTGGLQDLEFSKESKNSYSAEYLDDVLSLTKLSDYVKISFGNQKPLQLFFNMEGGGKVTYLLAPKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-04-27" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>P58017</accession>
    <name>Y6503_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>UPF0147 protein SSO6503</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO6503</name>
        <name type="ORF">C40_017.1</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0147 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="AAK41079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="H90227"/>
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    <dbReference type="EnsemblBacteria" id="AAK41079">
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    </dbReference>
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    <dbReference type="KEGG" id="sso:SSO6503"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.783"/>
    <dbReference type="eggNOG" id="arCOG04308">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165882_0_0_2"/>
    <dbReference type="InParanoid" id="P58017"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.50">
        <property type="entry name" value="Ta0600-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00342">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023130">
        <property type="entry name" value="Ta0600-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005354">
        <property type="entry name" value="UPF0147"/>
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    <dbReference type="NCBIfam" id="NF003319">
        <property type="entry name" value="PRK04330.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03685">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158436">
        <property type="entry name" value="Ta0600-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000150917" description="UPF0147 protein SSO6503">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="89" mass="9820" checksum="D496F58B9DE76CED" modified="2001-04-27" version="1">MSMPYDNEAKIKQAVILLQKIVNDTSVPRNIRRAATDAIRNLQDLGLSPAVRAANAIGILEDISQDPNMPTHARISIWNVVSILETVKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-08-14" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>P58222</accession>
    <name>RL18A_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L18Ae</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">50S ribosomal protein L20e</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">50S ribosomal protein LX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl18a</name>
        <name evidence="1" type="synonym">rpl20e</name>
        <name evidence="1" type="synonym">rplX</name>
        <name type="ordered locus">SSO5668</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Binds 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40680" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40680.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90178">
        <property type="entry name" value="A90178"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990642.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58222"/>
    <dbReference type="SMR" id="P58222"/>
    <dbReference type="FunCoup" id="P58222">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO5668"/>
    <dbReference type="PaxDb" id="273057-SSO5668"/>
    <dbReference type="EnsemblBacteria" id="AAK40680">
        <property type="protein sequence ID" value="AAK40680"/>
        <property type="gene ID" value="SSO5668"/>
    </dbReference>
    <dbReference type="GeneID" id="1455490"/>
    <dbReference type="GeneID" id="44129321"/>
    <dbReference type="KEGG" id="sso:SSO5668"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.341"/>
    <dbReference type="eggNOG" id="arCOG04175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177460_0_0_2"/>
    <dbReference type="InParanoid" id="P58222"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00273">
        <property type="entry name" value="Ribosomal_eL20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028877">
        <property type="entry name" value="Ribosomal_eL20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_eL20_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001981">
        <property type="entry name" value="PRK00773.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160374">
        <property type="entry name" value="RplX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000153710" description="Large ribosomal subunit protein eL20">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="86" mass="10232" checksum="70BE0114D82CD0A1" modified="2001-08-14" version="1">MSGIKFYLVKGTALFGESHYPEKRKFVKIVRALNEKQAIEYVYSHFGSKNKIKRYNIKIEQISEIKEEEIPDRRIRELAKVDKIIM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-08-01" modified="2025-06-18" version="144" xmlns="https://uniprot.org/uniprot">
    <accession>P80092</accession>
    <name>CBPX1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Thermostable carboxypeptidase 1</fullName>
            <ecNumber>3.4.17.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cpsA1</name>
        <name type="synonym">cpsA</name>
        <name type="synonym">cpsA-1</name>
        <name type="ordered locus">SSO1355</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1995" name="J. Bacteriol." volume="177" first="5561" last="5566">
            <title>Molecular cloning, nucleotide sequence, and expression of a carboxypeptidase-encoding gene from the archaebacterium Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Colombo S."/>
                <person name="Toietta G."/>
                <person name="Zecca L."/>
                <person name="Vanoni M."/>
                <person name="Tortora P."/>
            </authorList>
            <dbReference type="PubMed" id="7559343"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1992" name="Eur. J. Biochem." volume="206" first="349" last="357">
            <title>Purification and characterization of a thermostable carboxypeptidase from the extreme thermophilic archaebacterium Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Colombo S."/>
                <person name="D'Auria S."/>
                <person name="Fusi P."/>
                <person name="Zecca L."/>
                <person name="Raia C.A."/>
                <person name="Tortora P."/>
            </authorList>
            <dbReference type="PubMed" id="1597179"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1992.tb16934.x"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-31</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Can release basic, acidic, aromatic, and, to a lesser extent, aliphatic amino acids.</text>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text>Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.-"/>
    <dbReference type="EMBL" id="Z48497">
        <property type="protein sequence ID" value="CAA88397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90291">
        <property type="entry name" value="H90291"/>
    </dbReference>
    <dbReference type="PIR" id="S23180">
        <property type="entry name" value="S23180"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923348.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P80092"/>
    <dbReference type="SMR" id="P80092"/>
    <dbReference type="FunCoup" id="P80092">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1355"/>
    <dbReference type="MEROPS" id="M20.008"/>
    <dbReference type="PaxDb" id="273057-SSO1355"/>
    <dbReference type="EnsemblBacteria" id="AAK41591">
        <property type="protein sequence ID" value="AAK41591"/>
        <property type="gene ID" value="SSO1355"/>
    </dbReference>
    <dbReference type="GeneID" id="1454370"/>
    <dbReference type="GeneID" id="27427725"/>
    <dbReference type="KEGG" id="sso:SSO1355"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1361"/>
    <dbReference type="eggNOG" id="arCOG01108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023257_0_1_2"/>
    <dbReference type="InParanoid" id="P80092"/>
    <dbReference type="PhylomeDB" id="P80092"/>
    <dbReference type="BRENDA" id="3.4.17.B1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08021">
        <property type="entry name" value="M20_Acy1_YhaA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000014">
        <property type="entry name" value="N-acyl-L-amino acid amidohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053493">
        <property type="entry name" value="Thermostable_CP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040868">
        <property type="entry name" value="carboxypep_CpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014:SF63">
        <property type="entry name" value="METALLOPEPTIDASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G09600)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PEPTIDASE M20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0121">Carboxypeptidase</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000061947" description="Thermostable carboxypeptidase 1">
        <location>
            <begin position="1"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="373"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="sequence conflict" description="In Ref. 3; AA sequence." evidence="2" ref="3">
        <original>R</original>
        <variation>K</variation>
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 3; AA sequence." evidence="2" ref="3">
        <original>W</original>
        <variation>R</variation>
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 3; AA sequence." evidence="2" ref="3">
        <original>Y</original>
        <variation>N</variation>
        <location>
            <position position="28"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 3; AA sequence." evidence="2" ref="3">
        <original>E</original>
        <variation>P</variation>
        <location>
            <position position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="393" mass="43069" checksum="B4754EC5A8E9A9C8" modified="1996-10-01" version="2">MDLVEKLKNDVREIEDWIIQIRRKIHEYPELSYKEYNTSKLVAETLRKLGVEVEEGVGLPTAVVGKIRGSKPGKTVALRADMDALPVEENTDLEFKSKVKGVMHACGHDTHVAMLLGGAYLLVKNKDLISGEIRLIFQPAEEDGGLGGAKPMIEAGVMNGVDYVFGIHISSSYPSGVFATRKGPIMATPDAFKIIVHGKGGHGSAPHETIDPIFISLQIANAIYGITARQIDPVQPFIISITTIHSGTKDNIIPDDAEMQGTIRSLDENVRSKAKDYMRRIVSSICGIYGATCEVKFMEDVYPTTVNNPEVTDEVMKILSSISTVVETEPVLGAEDFSRFLQKAPGTYFFLGTRNEKKGCIYPNHSSKFCVDEDVLKLGALAHALLAVKFSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX53</accession>
    <name>Q7LX53_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73094">
        <property type="entry name" value="S73094"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923791.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX53"/>
    <dbReference type="STRING" id="273057.SSO2151"/>
    <dbReference type="PaxDb" id="273057-SSO2151"/>
    <dbReference type="EnsemblBacteria" id="AAK42327">
        <property type="protein sequence ID" value="AAK42327"/>
        <property type="gene ID" value="SSO2151"/>
    </dbReference>
    <dbReference type="GeneID" id="1453654"/>
    <dbReference type="KEGG" id="sso:SSO2151"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2244"/>
    <dbReference type="eggNOG" id="arCOG02021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1830677_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX53"/>
    <dbReference type="PhylomeDB" id="Q7LX53"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050229">
        <property type="entry name" value="GlpE_sulfurtransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43031">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43031:SF1">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="29"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="113" mass="13134" checksum="235A4FFB9DC2CF62" modified="2011-05-31" version="1">MTQVIESYSSPFYKNIIDLPPSMVRKLWKSGKIMILDIRTPQEYEEHHIPGAILAPLDYLEHLTELFEDKEVAVVCEHGNRARYATYGMPHLYKKRAYYMIGGMALWMCLPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXF7</accession>
    <name>Q7LXF7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90232">
        <property type="entry name" value="E90232"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923111.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXF7"/>
    <dbReference type="SMR" id="Q7LXF7"/>
    <dbReference type="STRING" id="273057.SSO0820"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO0820"/>
    <dbReference type="EnsemblBacteria" id="AAK41116">
        <property type="protein sequence ID" value="AAK41116"/>
        <property type="gene ID" value="SSO0820"/>
    </dbReference>
    <dbReference type="GeneID" id="1455083"/>
    <dbReference type="KEGG" id="sso:SSO0820"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.832"/>
    <dbReference type="eggNOG" id="arCOG01397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055387_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXF7"/>
    <dbReference type="PhylomeDB" id="Q7LXF7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916:SF3">
        <property type="entry name" value="UDP-GLCNAC:BETAGAL BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37649" checksum="FDDAA083C19CF647" modified="2011-05-31" version="1">MLRSTVIVTAHRRKAFIKDAIESVMENSLKPTEIIVVKNFKDYQIDSFLETYHVKNIYTDDETLGGKISLGIYQSSGDILFFLDDDDLFSKDKIREVIYKFSKYDIGFYHNAQEVIHNKLTGSNLSTDNQRFMYYTSIDTRRLKELLYKFKAGFNGSSIVISRDLAMRCVDLLKNVNISVDTFLLFCAVENNLPIMIDFRKLTYYRIIMSQNSNTEVNDKLFKMLYEDALYFKQIFSSKALRDFIEMSIIQHDFLYKIISKKEISKRDALISMVKMLRYSLKYPNKWNFFLQGLSLLSVFSAEKSRSLYLKKVRSNSMLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXJ1</accession>
    <name>Q7LXJ1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SSV1 integrase homolog (Orf D-335)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90228">
        <property type="entry name" value="A90228"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923075.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXJ1"/>
    <dbReference type="PaxDb" id="273057-SSO0782"/>
    <dbReference type="EnsemblBacteria" id="AAK41080">
        <property type="protein sequence ID" value="AAK41080"/>
        <property type="gene ID" value="SSO0782"/>
    </dbReference>
    <dbReference type="GeneID" id="1455047"/>
    <dbReference type="KEGG" id="sso:SSO0782"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.785"/>
    <dbReference type="eggNOG" id="arCOG01244">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083804_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXJ1"/>
    <dbReference type="PhylomeDB" id="Q7LXJ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031857">
        <property type="entry name" value="Integrase_SSV1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16795">
        <property type="entry name" value="Phage_integr_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase SSV1 C-terminal" evidence="2">
        <location>
            <begin position="68"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="226" mass="26873" checksum="6FBEAC1D489E79ED" modified="2011-05-31" version="1">MIKERGISEKTAREYVSALNRPYRETRNTQKAYRLFAKFLMSRGIISENFAEKILKIIKVKKANVDLYIPSIDEVRQTLEIAREYSENIYFIYRLALESGARLSEILTVLRNPDRDVCEDICYYPLSWERGFKRSFYIFHITQLKKIDITQWAVSDFEKRYDVVKIKYIRKFVATKMAELGIQLDIIDFIQGRKPSRVLTQHYVSLLGIAKENYKKYAEWLKNVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXK1</accession>
    <name>Q7LXK1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alcohol dehydrogenase (Zn containing) (Adh-2)</fullName>
            <ecNumber evidence="8">1.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">adh-2</name>
        <name evidence="8" type="ordered locus">SSO0764</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90225">
        <property type="entry name" value="F90225"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991352.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXK1"/>
    <dbReference type="FunCoup" id="Q7LXK1">
        <property type="interactions" value="189"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0764"/>
    <dbReference type="PaxDb" id="273057-SSO0764"/>
    <dbReference type="EnsemblBacteria" id="AAK41061">
        <property type="protein sequence ID" value="AAK41061"/>
        <property type="gene ID" value="SSO0764"/>
    </dbReference>
    <dbReference type="GeneID" id="1455028"/>
    <dbReference type="KEGG" id="sso:SSO0764"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.763"/>
    <dbReference type="eggNOG" id="arCOG01455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_0_2"/>
    <dbReference type="InParanoid" id="Q7LXK1"/>
    <dbReference type="PhylomeDB" id="Q7LXK1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030554">
        <property type="term" value="F:adenyl nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043168">
        <property type="term" value="F:anion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08259">
        <property type="entry name" value="Zn_ADH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053496">
        <property type="entry name" value="Acryloyl-CoA_Reductase_Zn-ADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041172">
        <property type="entry name" value="AcrlCoa_red_Thmprot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010344">
        <property type="entry name" value="PRK13771.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF4">
        <property type="entry name" value="D-ARABINOSE 1-DEHYDROGENASE (NADP(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="8"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="333" mass="36061" checksum="449152D5C1B7F38D" modified="2011-05-31" version="1">MKAVVVPGKQKGYRIEEVSDPIPGKDEVVIRVNRAALCYRDLLQLQGFYPRMKYPVILGHEVVGTIEQIGENAKGFKEGDKVISLLYAPDGTCHYCQMGEEAYCHSRVGYSEELDGFFAEKAKVKVTSIVKVGTNVPDEGAVLVPCVTGMVYRSLRRAKLQKGETVLVTGASGGVGIHAIQVAKALGAKVIGVTTSEEKAAFIRKFADHVIVGSKFSEEAKKIGDVNVVIDTVGTPTIDESLKSLWMGGRLVQIGNVDPTQIYQLRLGYVILKDIAIIGHASATKRDAEETLKLTGEGKINPVIAATVGLDEIDKGYEILKDKHKIGKVLVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXS7</accession>
    <name>Q7LXS7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zn finger protein, hypothetical</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90207">
        <property type="entry name" value="E90207"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991130.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXS7"/>
    <dbReference type="STRING" id="273057.SSO0605"/>
    <dbReference type="PaxDb" id="273057-SSO0605"/>
    <dbReference type="EnsemblBacteria" id="AAK40916">
        <property type="protein sequence ID" value="AAK40916"/>
        <property type="gene ID" value="SSO0605"/>
    </dbReference>
    <dbReference type="GeneID" id="1454883"/>
    <dbReference type="KEGG" id="sso:SSO0605"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.613"/>
    <dbReference type="eggNOG" id="arCOG00580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166550_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXS7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase II subunit RPB9-like zinc ribbon" evidence="1">
        <location>
            <begin position="16"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="116" mass="13632" checksum="A85976E7D2842E9F" modified="2011-05-31" version="1">MQKFLYYSLTLFTLMKFCPKCGGLMVPSKKDGKEILKCSKCGYEMMLSEKEKKEYSVKESKDKSSKVLTTSIVSDKEGRNLSEELEHEREEYYKEIGLELLREEFEGNEEEESRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXT6</accession>
    <name>Q7LXT6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GTP binding protein, hypothetical</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO0581</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class YlqF/YawG GTPase family. MTG1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90204">
        <property type="entry name" value="G90204"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991096.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXT6"/>
    <dbReference type="FunCoup" id="Q7LXT6">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0581"/>
    <dbReference type="PaxDb" id="273057-SSO0581"/>
    <dbReference type="EnsemblBacteria" id="AAK40894">
        <property type="protein sequence ID" value="AAK40894"/>
        <property type="gene ID" value="SSO0581"/>
    </dbReference>
    <dbReference type="GeneID" id="1454861"/>
    <dbReference type="KEGG" id="sso:SSO0581"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.589"/>
    <dbReference type="eggNOG" id="arCOG00350">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011106_1_2_2"/>
    <dbReference type="InParanoid" id="Q7LXT6"/>
    <dbReference type="PhylomeDB" id="Q7LXT6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01859">
        <property type="entry name" value="MJ1464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016478">
        <property type="entry name" value="GTPase_MTG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF30">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006230">
        <property type="entry name" value="MG442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="4">
        <location>
            <begin position="2"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR006230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="267" mass="30452" checksum="757D72321F00CCC4" modified="2011-05-31" version="1">MLGKVIQLIKRSDLVVEVLDAREPSLTRSKKIEDIIVKNYKNILLVLNKGDLVPLWVLKAWKDYFKTEENIESVYVSATSHLGTKILRDTMKSLLKGNKGIVVFVGYPKSGKSSVINALKGRHSAQTSAHPLEYGYTKSIQLFKIDNKIYAWDTPGVIPPDGNELEKTIRGYNADLLEDPVKPALMLINRVIEFSKESLISVYKVDFNNPYELLERIAIRRGWFYKSTKEPNIDMAARAVIRDYHEGKIAYYTLPPSLYRDDKSNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXW8</accession>
    <name>Q7LXW8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF5658 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90198">
        <property type="entry name" value="B90198"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988374.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXW8"/>
    <dbReference type="STRING" id="273057.SSO0523"/>
    <dbReference type="PaxDb" id="273057-SSO0523"/>
    <dbReference type="EnsemblBacteria" id="AAK40841">
        <property type="protein sequence ID" value="AAK40841"/>
        <property type="gene ID" value="SSO0523"/>
    </dbReference>
    <dbReference type="GeneID" id="1454808"/>
    <dbReference type="KEGG" id="sso:SSO0523"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.519"/>
    <dbReference type="eggNOG" id="arCOG07282">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2204230_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXW8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13525" checksum="5F4838059F424C00" modified="2011-05-31" version="1">MRILKIVWVLFILLNVYDVVISAIYAIPGTITDGAVWETNLIISLYAYYEGSISFVLAVLMLISLKLLLFTGVYWYTKLFDLLKVSKYKWLSLLPFIAVSFLVDLGSTSVLISQHIPPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXX2</accession>
    <name>Q7LXX2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0519</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90197">
        <property type="entry name" value="F90197"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXX2"/>
    <dbReference type="STRING" id="273057.SSO0519"/>
    <dbReference type="PaxDb" id="273057-SSO0519"/>
    <dbReference type="EnsemblBacteria" id="AAK40837">
        <property type="protein sequence ID" value="AAK40837"/>
        <property type="gene ID" value="SSO0519"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0519"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.513"/>
    <dbReference type="eggNOG" id="arCOG07848">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1105249_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXX2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="243" mass="26343" checksum="F4E3E2852EC243A3" modified="2011-05-31" version="1">MKWFLLLLLVFGVLGIIPITNGVITGPHPQFDSGGGFAGPFFTYSKTMTIQSGQSQTKNNGSTLSSAQWLNPSYVSIYNTYYLQILPNQEYISNNVSFSLSASQIAINVTFLLASSSNSGAFGSLAIGYGTYIPSTFTNAFAPSVPSSGITVYLMKGGMNTYHLFVYFDGVKQLNVSVGSISIGEQIGLGFYYLPASNQLYVYYYNGSVKTFSLTPSQVVGTNVLGVFSNSHEIRKTFVKILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXY0</accession>
    <name>Q7LXY0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">thymine-DNA glycosylase</fullName>
            <ecNumber evidence="12">3.2.2.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ntH-1</name>
        <name evidence="15" type="ordered locus">SSO0116</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free thymine.</text>
            <dbReference type="EC" id="3.2.2.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.29" evidence="12"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75373">
        <property type="entry name" value="S75373"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988936.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXY0"/>
    <dbReference type="FunCoup" id="Q7LXY0">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0116"/>
    <dbReference type="PaxDb" id="273057-SSO0116"/>
    <dbReference type="EnsemblBacteria" id="AAK40470">
        <property type="protein sequence ID" value="AAK40470"/>
        <property type="gene ID" value="SSO0116"/>
    </dbReference>
    <dbReference type="GeneID" id="1453298"/>
    <dbReference type="KEGG" id="sso:SSO0116"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.110"/>
    <dbReference type="eggNOG" id="arCOG00459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_3_4_2"/>
    <dbReference type="InParanoid" id="Q7LXY0"/>
    <dbReference type="PhylomeDB" id="Q7LXY0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141016">
        <property type="term" value="F:G/T mismatch-specific thymine-DNA glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000001">
        <property type="entry name" value="Endonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004035">
        <property type="entry name" value="Endouclease-III_FeS-bd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359">
        <property type="entry name" value="A/G-SPECIFIC ADENINE GLYCOSYLASE/ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359:SF18">
        <property type="entry name" value="ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001435">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00764">
        <property type="entry name" value="ENDONUCLEASE_III_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="15" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="14">
        <location>
            <begin position="44"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Helix-hairpin-helix DNA-binding motif class 1" evidence="13">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Helix-hairpin-helix DNA-binding motif class 1" evidence="13">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAK40470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="236" mass="26843" checksum="6FF5CADB280ECCD2" modified="2011-05-31" version="1">MKCTAETIFHKLSATYIIKEEDFIAYYVWLKTKDCFKVLVATILSQNSTDKSAIKAYLELERKVGVTPEKLSNANLADIESALKISGLYRTKAKRLKEISRIILERYNGLIDSLLNTSNARDELLKLEGIGEKTADVVLLTCYGYYGYKVFPVDTHITRVSKRLGIVPTNAKYSLISSTLKELFSAYDLLHLHHMLIAHGRQTCKARKPLCNSCIIKECCEYYSHRDGEAWRSNTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXY8</accession>
    <name>Q7LXY8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alcohol dehydrogenase GroES domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75383">
        <property type="entry name" value="S75383"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988924.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXY8"/>
    <dbReference type="STRING" id="273057.SSO0106"/>
    <dbReference type="PaxDb" id="273057-SSO0106"/>
    <dbReference type="EnsemblBacteria" id="AAK40461">
        <property type="protein sequence ID" value="AAK40461"/>
        <property type="gene ID" value="SSO0106"/>
    </dbReference>
    <dbReference type="GeneID" id="1453289"/>
    <dbReference type="KEGG" id="sso:SSO0106"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.102"/>
    <dbReference type="eggNOG" id="arCOG05990">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978659_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXY8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="31971" checksum="A6300912EF6F76AE" modified="2011-05-31" version="1">MKAVVFNLGITISDVPEKQVNRDYVLLKPKRVLVNGLENSIYVGLLWVDPTRILGSTGIGRIEGVGLDIDKSLEGKLVLVLPYSQTYGGIGTEIDGILAEKASVPLDSIVILPQYGFNEKYILYPYVSFALQLPKYIHSGNTLIIGSGLYGIISALYLKDMVSKVAVYREDGINPKIVGVEEIRHFSQEWDNIIITTFRSWIRAFIDDISKSNTKIVMPKLMNTWPVVSSTKTVFIPPKEVDGVLEFIDKKINDKLFNELVSFSNDLLVSFPSPKAGVVINAEEIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXZ3</accession>
    <name>Q7LXZ3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO0087</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75400">
        <property type="entry name" value="S75400"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXZ3"/>
    <dbReference type="FunCoup" id="Q7LXZ3">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0087"/>
    <dbReference type="PaxDb" id="273057-SSO0087"/>
    <dbReference type="EnsemblBacteria" id="AAK40447">
        <property type="protein sequence ID" value="AAK40447"/>
        <property type="gene ID" value="SSO0087"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0087"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.86"/>
    <dbReference type="eggNOG" id="arCOG04064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042134_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LXZ3"/>
    <dbReference type="PhylomeDB" id="Q7LXZ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031293">
        <property type="term" value="P:membrane protein intracellular domain proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06159">
        <property type="entry name" value="S2P-M50_PDZ_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001193">
        <property type="entry name" value="MBTPS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325:SF3">
        <property type="entry name" value="MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325">
        <property type="entry name" value="PROTEASE M50 MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE 2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01000">
        <property type="entry name" value="SREBPS2PTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="311"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="230"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="386" mass="43424" checksum="25D3DF85819DA08D" modified="2011-05-31" version="1">MPMTITILYLDIFLISKYIIRFVDQIYLAVALFLSFWLIIYVFRRRLEKYNLTVYPFFILWRKKSREYWFPKFSRSKGYRAYEKIALPIGFLLMIAGIVTILYIIIEMLTIKPNQTPTIALKPIIPGVTISISQLPYILLAIGVSVAIHEIFHALSATSNNVKVKNGGVLLLGIFPGAFVEPDEDDFNKSTSNAKLKIIAAGIVINLVLALIALPLSFELPYLPSALSQGIIIEGVLNNTPAANASLHTGDIIYSINGYRLTTLSQLHELLYNYSTITITLKHPNGSLSNVSVNIPNHFLGVYVTYYIPDYIAAILMFFTWLFIVNFSLAVFNAAPLIITDGGKLLTELLKRMLGESNGEKISYYLQSLFLLIFIFAIFLSNRPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXZ4</accession>
    <name>Q7LXZ4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">protein adenylyltransferase</fullName>
            <ecNumber evidence="9">2.7.7.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">SSO0086</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-tyrosyl-[protein] + ATP = O-(5'-adenylyl)-L-tyrosyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-(5'-adenylyl)-L-tyrosyl-[protein] + ATP = O-[5'-(adenylyl-(5'-&gt;3')-adenylyl)]-L-tyrosyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:66528"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17046"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="ChEBI" id="CHEBI:167160"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the MntA antitoxin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.108" evidence="9"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75402">
        <property type="entry name" value="S75402"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922879.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXZ4"/>
    <dbReference type="STRING" id="273057.SSO0086"/>
    <dbReference type="PaxDb" id="273057-SSO0086"/>
    <dbReference type="DNASU" id="1453274"/>
    <dbReference type="EnsemblBacteria" id="AAK40446">
        <property type="protein sequence ID" value="AAK40446"/>
        <property type="gene ID" value="SSO0086"/>
    </dbReference>
    <dbReference type="GeneID" id="1453274"/>
    <dbReference type="KEGG" id="sso:SSO0086"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.85"/>
    <dbReference type="eggNOG" id="arCOG04066">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1217517_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXZ4"/>
    <dbReference type="PhylomeDB" id="Q7LXZ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070733">
        <property type="term" value="F:AMPylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009185">
        <property type="entry name" value="Nucleotidl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052038">
        <property type="entry name" value="Type-VII_TA_antitoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33571:SF14">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE MJ0435-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33571">
        <property type="entry name" value="SSL8005 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005928">
        <property type="entry name" value="Nucleotidltrnsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="13">
        <location>
            <begin position="4"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAK40446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="187" mass="21424" checksum="E7C372B7C6E4F4E9" modified="2011-05-31" version="1">MEGYVYGSVARGDVNEKSDVDIIVFNPNQIVLDTLNVNHKYLIQATPNSVPKAYLSLDEEETIVISFPLGRLRRNEIEFYSFGGLVDLKGLSKNERVPGVNKKLMLIIPTENGHMEIPLEGNEDYASKLLKISLETIMERKRLLARRMERGHTGVFLRYDLGGNESIYDAFNKMYKSNKFFKRMVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TW9</accession>
    <name>Q97TW9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Second ORF in partial transposon ISC1491</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1491</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO7469</name>
        <name evidence="3" type="ordered locus">SSO7475</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90278">
        <property type="entry name" value="E90278"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TW9"/>
    <dbReference type="STRING" id="273057.SSO7469"/>
    <dbReference type="PaxDb" id="273057-SSO7469"/>
    <dbReference type="EnsemblBacteria" id="AAK41480">
        <property type="protein sequence ID" value="AAK41480"/>
        <property type="gene ID" value="SSO7469"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41484">
        <property type="protein sequence ID" value="AAK41484"/>
        <property type="gene ID" value="SSO7475"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7469"/>
    <dbReference type="KEGG" id="sso:SSO7475"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1234"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153635_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF17">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="97" mass="10936" checksum="08EE6B569E3BFE6F" modified="2001-10-01" version="1">MISAATIYAEFGDVSRFSSSKARVYAGFAPRTKQSGESESHSGMGNKYLRRAFFLVARVARKSERFKEYYDRLVSKWKSATKLVLLVGSLLVFVITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U62</accession>
    <name>Q97U62_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoate-protein ligase A (LplA-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lplA-2</name>
        <name evidence="2" type="ordered locus">SSO3159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90500">
        <property type="entry name" value="E90500"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U62"/>
    <dbReference type="FunCoup" id="Q97U62">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3159"/>
    <dbReference type="PaxDb" id="273057-SSO3159"/>
    <dbReference type="EnsemblBacteria" id="AAK43260">
        <property type="protein sequence ID" value="AAK43260"/>
        <property type="gene ID" value="SSO3159"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3159"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3265"/>
    <dbReference type="eggNOG" id="arCOG01939">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022986_5_0_2"/>
    <dbReference type="InParanoid" id="Q97U62"/>
    <dbReference type="PhylomeDB" id="Q97U62"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033819">
        <property type="term" value="F:lipoyl(octanoyl) transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009107">
        <property type="term" value="P:lipoate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16443">
        <property type="entry name" value="LplA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050664">
        <property type="entry name" value="Octanoyltrans_LipM/LipL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679:SF2">
        <property type="entry name" value="OCTANOYL-[GCVH]:PROTEIN N-OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679">
        <property type="entry name" value="OCTANOYLTRANSFERASE LIPM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="30"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="264" mass="29593" checksum="BFF9C35480B4C816" modified="2001-10-01" version="1">MKMLRIIVDGPRDPYYNMAIDEAIMLTRNSVNYDTLRLYMWSPSGVSLGRGQNANAAYLDNIKELGFKLVRRPTGGGALLHPEDNEITYSVVLSASNTIAKLSVDESAVEIAKGILYTLQILGENVDIRGLGDMKKHDLCYLRSGSSDVIVAGRKISGSAQVRDDKALLQHGTLLLRFEPEIWLKVIRAPGVTEEFLKSRIVGLFEFMEVEIDQIFDAMIKGFSKALNEIDVFMGSLTPKEIKLSNKLYKEKYSNEKWNLLGIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U87</accession>
    <name>Q97U87_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISC1359</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO3135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90497">
        <property type="entry name" value="C90497"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U87"/>
    <dbReference type="STRING" id="273057.SSO3135"/>
    <dbReference type="PaxDb" id="273057-SSO3135"/>
    <dbReference type="EnsemblBacteria" id="AAK43234">
        <property type="protein sequence ID" value="AAK43234"/>
        <property type="gene ID" value="SSO3135"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3135"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3240"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067987_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U87"/>
    <dbReference type="PhylomeDB" id="Q97U87"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033541">
        <property type="entry name" value="transpos_ISH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="89"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="332" mass="38436" checksum="F59E4D177AEDCD9B" modified="2001-10-01" version="1">MKHENTTKPAKFNRDFARSALKIIYSVLTKILFPEELLSALLKASGSYLSRLGKDGRRALRKLNAVQVEDVRDALRKMGRMTLRGVRDRRVAVDFHAIPQYHADKSFLSRIKPTKGTSWGLVQAAIFLLGRTRSFLDVIPVTVKNVAEGFKAVMEVIVKELEEDKLRLVMVFADREFAVNEVIRFLLELGLDFVISAKAQMYKKYKGMLQDVDVSFGGVRYTGFLCVRHGSGAYLIILRKEDGKIIAFLVRREMDLYDAIVLAEMYRERWGIENAFRSLEEFRVRTRTCDVRKELVLVLLSYLLLNVWFLIRSWRKVKLWEFSVSLSNLLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UC2</accession>
    <name>Q97UC2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter, ATP binding protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90492">
        <property type="entry name" value="H90492"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989119.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UC2"/>
    <dbReference type="STRING" id="273057.SSO3093"/>
    <dbReference type="PaxDb" id="273057-SSO3093"/>
    <dbReference type="EnsemblBacteria" id="AAK43199">
        <property type="protein sequence ID" value="AAK43199"/>
        <property type="gene ID" value="SSO3093"/>
    </dbReference>
    <dbReference type="GeneID" id="1453118"/>
    <dbReference type="KEGG" id="sso:SSO3093"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3201"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1025260_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UC2"/>
    <dbReference type="PhylomeDB" id="Q97UC2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423:SF1">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="2"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="237" mass="26998" checksum="B3734F93E340AB36" modified="2001-10-01" version="1">MIRAINVSKIFHGSSAVVEVNLDIYENEKVGVIGVHNSGKTVLLQMLAGDVKPTKGKVYLEDKRKIAYIPQVPKFIPTISVKDVMTYVNKEYHYLDFVGLDGNKKVKDLSLDEKKRLSLALSLPFSPNHLIVDDMSQISSLTKDLIRRFKGGVIIAHHNLKDIWDLIDRVIIMSKGRVVFDGPKSGLLYKVIRFKNSEIWERENENSIEIDLDKCGVKYEVIRVTPDEVFLYFYAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UF6</accession>
    <name>Q97UF6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Long-chain-fatty-acid--CoA ligase (FadD-3)</fullName>
            <ecNumber evidence="3">6.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fadD-3</name>
        <name evidence="3" type="ordered locus">SSO3064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90488">
        <property type="entry name" value="F90488"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990879.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UF6"/>
    <dbReference type="FunCoup" id="Q97UF6">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3064"/>
    <dbReference type="PaxDb" id="273057-SSO3064"/>
    <dbReference type="EnsemblBacteria" id="AAK43165">
        <property type="protein sequence ID" value="AAK43165"/>
        <property type="gene ID" value="SSO3064"/>
    </dbReference>
    <dbReference type="GeneID" id="7798452"/>
    <dbReference type="KEGG" id="sso:SSO3064"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3165"/>
    <dbReference type="eggNOG" id="arCOG00856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_9_2"/>
    <dbReference type="InParanoid" id="Q97UF6"/>
    <dbReference type="PhylomeDB" id="Q97UF6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050237">
        <property type="entry name" value="ATP-dep_AMP-bd_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767:SF1">
        <property type="entry name" value="NONRIBOSOMAL PEPTIDE SYNTHASE PES1 (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="2">
        <location>
            <begin position="425"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="513" mass="58913" checksum="9AF3FF8CD22058E9" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UN1</accession>
    <name>Q97UN1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sulfocyanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">soxE</name>
        <name evidence="6" type="ordered locus">SSO2972</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90477">
        <property type="entry name" value="F90477"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992568.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UN1"/>
    <dbReference type="STRING" id="273057.SSO2972"/>
    <dbReference type="PaxDb" id="273057-SSO2972"/>
    <dbReference type="EnsemblBacteria" id="AAK43077">
        <property type="protein sequence ID" value="AAK43077"/>
        <property type="gene ID" value="SSO2972"/>
    </dbReference>
    <dbReference type="GeneID" id="1452996"/>
    <dbReference type="KEGG" id="sso:SSO2972"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3063"/>
    <dbReference type="eggNOG" id="arCOG03700">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105306_1_0_2"/>
    <dbReference type="InParanoid" id="Q97UN1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04230">
        <property type="entry name" value="Sulfocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010532">
        <property type="entry name" value="SoxE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049544">
        <property type="entry name" value="SoxE-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055193">
        <property type="entry name" value="SoxE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034246">
        <property type="entry name" value="Sulfocyanin_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03094">
        <property type="entry name" value="sulfo_cyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06525">
        <property type="entry name" value="SoxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22852">
        <property type="entry name" value="SoxE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022145">
        <property type="entry name" value="Sulfocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfocyanin N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfocyanin-like C-terminal" evidence="4">
        <location>
            <begin position="49"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="NCBIfam" id="TIGR03094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="197" mass="20722" checksum="8B96464549CC9794" modified="2001-10-01" version="1">MKAQSSLLPVIVGVLVAIVAVGVSVYAYYEYQVLSAPTSTATSTSTSTSSQIPLKYDSTNKTVFLIISVLTTGPTFNFNGTSNGQLKIYIPAGWSVYVKFVNEQSLPHNLILLQNTTATPSNPDVGQFGKILYIVGATTSNYQTSGISSGQSDSGLWGPLSAGTYMLVCGILGHAQSGMWAVVIVSSNVTTPYTTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UP1</accession>
    <name>Q97UP1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90476">
        <property type="entry name" value="D90476"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924073.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UP1"/>
    <dbReference type="STRING" id="273057.SSO2963"/>
    <dbReference type="PaxDb" id="273057-SSO2963"/>
    <dbReference type="EnsemblBacteria" id="AAK43067">
        <property type="protein sequence ID" value="AAK43067"/>
        <property type="gene ID" value="SSO2963"/>
    </dbReference>
    <dbReference type="GeneID" id="1452986"/>
    <dbReference type="KEGG" id="sso:SSO2963"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3053"/>
    <dbReference type="eggNOG" id="arCOG07335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1567184_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UP1"/>
    <dbReference type="PhylomeDB" id="Q97UP1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="56"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="193" mass="22481" checksum="9696C0ABAE166F69" modified="2001-10-01" version="1">MSMFENTHPELTRIGIRIENVDSRYKPLMRMGISIDEMIYLARRLYPLIRDGKRILDLGCGNGLITVILSKLISEAEFHAVDDWKKVSRDDLIKNIEIDKAKIALGEFTDQFTLSYQDTYFDFVYSVMFLSNIGKDGRTKISDEVHRVLKEKGKFVVIDTLVFRGKIKKDLGDKFKLDWYGEENGFSFFVWSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UP2</accession>
    <name>Q97UP2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO11571</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90476">
        <property type="entry name" value="C90476"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UP2"/>
    <dbReference type="STRING" id="273057.SSO11571"/>
    <dbReference type="PaxDb" id="273057-SSO11571"/>
    <dbReference type="EnsemblBacteria" id="AAK43066">
        <property type="protein sequence ID" value="AAK43066"/>
        <property type="gene ID" value="SSO11571"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11571"/>
    <dbReference type="HOGENOM" id="CLU_2968630_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UP2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="58" mass="6696" checksum="A14848BAD30D3531" modified="2001-10-01" version="1">MSLYQGHGNYYFSEVFTTVIIFSLGNTRKPLSWRKYLNSLDPPMKRYESTDNSAGKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UR8</accession>
    <name>Q97UR8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial transposase ISC1078</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2933</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90472">
        <property type="entry name" value="G90472"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UR8"/>
    <dbReference type="STRING" id="273057.SSO2933"/>
    <dbReference type="PaxDb" id="273057-SSO2933"/>
    <dbReference type="EnsemblBacteria" id="AAK43038">
        <property type="protein sequence ID" value="AAK43038"/>
        <property type="gene ID" value="SSO2933"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2933"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3022"/>
    <dbReference type="eggNOG" id="arCOG02127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1718298_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UR8"/>
    <dbReference type="PhylomeDB" id="Q97UR8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051839">
        <property type="entry name" value="RD_transcriptional_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025959">
        <property type="entry name" value="Winged_HTH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33215">
        <property type="entry name" value="PROTEIN DISTAL ANTENNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33215:SF13">
        <property type="entry name" value="PROTEIN DISTAL ANTENNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13592">
        <property type="entry name" value="HTH_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Winged helix-turn helix" evidence="1">
        <location>
            <begin position="89"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="137" mass="16154" checksum="D7C19EB85AF67722" modified="2001-10-01" version="1">MPKSRMHGIRLNKKQEEERRINAVKDVRNGMSIKDVAKKYDVSIFTVYKWLRKGDLSAKPRKGPTKLKDEEKLVRILEKSPRDFGLNYDFWTLKLIAYILDKEFGIKYNPRSLSPVLKKLGFKYKKGKRTYVRVSGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UR9</accession>
    <name>Q97UR9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1234</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2929</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90472">
        <property type="entry name" value="D90472"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UR9"/>
    <dbReference type="STRING" id="273057.SSO2929"/>
    <dbReference type="PaxDb" id="273057-SSO2929"/>
    <dbReference type="EnsemblBacteria" id="AAK43035">
        <property type="protein sequence ID" value="AAK43035"/>
        <property type="gene ID" value="SSO2929"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2929"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3019"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075479_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UR9"/>
    <dbReference type="PhylomeDB" id="Q97UR9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="202" mass="23086" checksum="B347B930C374DFC8" modified="2001-10-01" version="1">MNVVVAWFVGRKVGKSTLHDFVGRLYGVRKKLLEISFKLEEKCLPSYLPASAHLVDFMAWLVDSFLLDLPPGKRSVETFREKAELERREGNLERARKLLSLGRTKRRFEGRWTKKRGVSHYGLKAVAVISVSLFVRSITLKPANFSDKRFKSPLKGIKIADRGFSPSPTQLIAREKPFITLRAHVEFFGTYLNASVSEKFID</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UV0</accession>
    <name>Q97UV0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2892</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99468">
        <property type="entry name" value="A99468"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990803.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UV0"/>
    <dbReference type="FunCoup" id="Q97UV0">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2892"/>
    <dbReference type="PaxDb" id="273057-SSO2892"/>
    <dbReference type="EnsemblBacteria" id="AAK43000">
        <property type="protein sequence ID" value="AAK43000"/>
        <property type="gene ID" value="SSO2892"/>
    </dbReference>
    <dbReference type="GeneID" id="1452919"/>
    <dbReference type="KEGG" id="sso:SSO2892"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2980"/>
    <dbReference type="eggNOG" id="arCOG01064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030742_5_0_2"/>
    <dbReference type="InParanoid" id="Q97UV0"/>
    <dbReference type="PhylomeDB" id="Q97UV0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052541">
        <property type="entry name" value="SQRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755:SF1">
        <property type="entry name" value="FAD-DEPENDENT PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="3"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="367" mass="40278" checksum="A3AEAA8EBF6C68ED" modified="2001-10-01" version="1">MKRLVVVGGGNAGTLIANLLADKLEVTVIEPNEYHTYQPGLVDYITGEVDENKIKMPTSSLLKPSVKWVRSKATKIMPENRTIISESGEKFEGDYIVIATGGQNIPVHSLRGYHTIEDAKMIKNEVMNPRGKSFVIGSLPGIIKCPAAPWELSFLIKRKYPDANVSVIVPAKQPPPLQVPMSNMFNKKANELGIKVYKGFVIQEVNYNERYAVSTEGEKIAFDHLIIDTPISTGFSELADKSGLIPVDKSTLAYKDGVFVIGDANSTPTPKTGAAAHFQAEVVANNILAEIEGNRSKESYDGTAICAVYSGPNEGMLVWLNYEKSKALGPTSIYRYMKKAFTRLYWASLSGGVDFILKPLVESIRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UV5</accession>
    <name>Q97UV5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO11389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90467">
        <property type="entry name" value="D90467"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UV5"/>
    <dbReference type="STRING" id="273057.SSO11389"/>
    <dbReference type="PaxDb" id="273057-SSO11389"/>
    <dbReference type="EnsemblBacteria" id="AAK42995">
        <property type="protein sequence ID" value="AAK42995"/>
        <property type="gene ID" value="SSO11389"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11389"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2519933_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UV5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025471">
        <property type="entry name" value="DUF4322"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14210">
        <property type="entry name" value="DUF4322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4322" evidence="1">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="84" mass="9890" checksum="2FA96C7C179D7100" modified="2001-10-01" version="1">MTRTQLIEDRFKVRNPVLSRGEDKVITPGLPHQNNIQQVGYKLLSMLSFKEERLRRYREFWSPRACGTTPWKASPKGITCHHRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UY5</accession>
    <name>Q97UY5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO11307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90463">
        <property type="entry name" value="D90463"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UY5"/>
    <dbReference type="PaxDb" id="273057-SSO11307"/>
    <dbReference type="EnsemblBacteria" id="AAK42963">
        <property type="protein sequence ID" value="AAK42963"/>
        <property type="gene ID" value="SSO11307"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11307"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_207591_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UY5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="48" mass="5910" checksum="2C9044CCEBD5C0A5" modified="2001-10-01" version="1">MRKLTNKIFLYLYYTVLDSVWNLVNYLLFNFKSTKRKPLSFDSFVRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V39</accession>
    <name>Q97V39_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2793</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90456">
        <property type="entry name" value="C90456"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991947.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V39"/>
    <dbReference type="STRING" id="273057.SSO2793"/>
    <dbReference type="PaxDb" id="273057-SSO2793"/>
    <dbReference type="EnsemblBacteria" id="AAK42906">
        <property type="protein sequence ID" value="AAK42906"/>
        <property type="gene ID" value="SSO2793"/>
    </dbReference>
    <dbReference type="GeneID" id="1452825"/>
    <dbReference type="KEGG" id="sso:SSO2793"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2879"/>
    <dbReference type="eggNOG" id="arCOG01505">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1187825_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V39"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3480.10">
        <property type="entry name" value="TorD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020945">
        <property type="entry name" value="DMSO/NO3_reduct_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036411">
        <property type="entry name" value="TorD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050289">
        <property type="entry name" value="TorD/DmsD_chaperones"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34227">
        <property type="entry name" value="CHAPERONE PROTEIN YCDY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34227:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE CHAPERONE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02613">
        <property type="entry name" value="Nitrate_red_del"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89155">
        <property type="entry name" value="TorD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="25261" checksum="31BF2201902B1613" modified="2001-10-01" version="1">MALSVLLNIRHASYDLFADLFLYKFGEDEYKQLLEKIDLVDKELGKQLEETIGIRVNEIKRVFSETKRSGYLIEYSTLFLSGFGAKPLLPVESKRLFQLMGEKVATFKYTDVINFYRSRGIVMRFSSQFNPEPDHISSIFAFMSLLIEEEYEQRSEGKDPFKVLQDEKNFAITHIFSWIPDWASDVINDPRSSIYKVVCSELAKFLRFERKYLGVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VG8</accession>
    <name>Q97VG8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Quinol oxidase (SoxABC), subunit II (SoxA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">soxA</name>
        <name evidence="3" type="ordered locus">SSO2658</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99440">
        <property type="entry name" value="A99440"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988570.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VG8"/>
    <dbReference type="FunCoup" id="Q97VG8">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2658"/>
    <dbReference type="PaxDb" id="273057-SSO2658"/>
    <dbReference type="EnsemblBacteria" id="AAK42776">
        <property type="protein sequence ID" value="AAK42776"/>
        <property type="gene ID" value="SSO2658"/>
    </dbReference>
    <dbReference type="GeneID" id="1452695"/>
    <dbReference type="GeneID" id="44128386"/>
    <dbReference type="KEGG" id="sso:SSO2658"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2740"/>
    <dbReference type="eggNOG" id="arCOG01236">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582965_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VG8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13842">
        <property type="entry name" value="CuRO_HCO_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053624">
        <property type="entry name" value="Cytochrome_c_oxidase_su2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041074">
        <property type="entry name" value="quin_ox_SoxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit II copper A binding" evidence="2">
        <location>
            <begin position="74"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="169" mass="19105" checksum="37E38CFB971B6F9D" modified="2001-10-01" version="1">MDIKEHAEEVWFIIMLILVATFFSWNVYYILTGKSFSLRYGLPEFSGLPPQAQEIVKYFEAHPPPSGEYSEVINGTLVVNLTAVQYRWIPNVIIANVSEPVVLIINSPQVDTGFYLRLPNGLINVNNVPGITSYVYFVTPNQPGNYTWYNAEYDGYSSSYMTGTLEVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VJ9</accession>
    <name>Q97VJ9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl-CoA c-acetyltransferase (Acetoacetyl-CoA thiolase) (AcaB-7)</fullName>
            <ecNumber evidence="4">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">acaB-7</name>
        <name evidence="4" type="ordered locus">SSO2625</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.9" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90436">
        <property type="entry name" value="B90436"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989351.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VJ9"/>
    <dbReference type="FunCoup" id="Q97VJ9">
        <property type="interactions" value="318"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2625"/>
    <dbReference type="PaxDb" id="273057-SSO2625"/>
    <dbReference type="EnsemblBacteria" id="AAK42745">
        <property type="protein sequence ID" value="AAK42745"/>
        <property type="gene ID" value="SSO2625"/>
    </dbReference>
    <dbReference type="GeneID" id="1452664"/>
    <dbReference type="KEGG" id="sso:SSO2625"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2707"/>
    <dbReference type="eggNOG" id="arCOG01278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_2"/>
    <dbReference type="InParanoid" id="Q97VJ9"/>
    <dbReference type="PhylomeDB" id="Q97VJ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004720">
        <property type="entry name" value="PRK06064.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009228">
        <property type="entry name" value="PRK12578.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF6">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="3">
        <location>
            <begin position="242"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="387" mass="41892" checksum="4F6E505FEDB3522F" modified="2001-10-01" version="1">MTRRVAVIGVGNSKFGRRDDVSIQELAWESIKEALNDSGVSQSDIGLVVIGSTAYRGIELYPAPIVAEYSGLTGKVPLRVEAMCATGLAAALSAYTAVASGLVDIAMAVGVDKMTEVDTSTSLAIGGRGGNYQWEYHFYGTTFPTYYALYATRHMAVFGTTEEQMALVSVKAHKYGSMNPKAHFQKPVTVEEVLKSRVISWPIKLLDSCPISDGSATAVFASEEKVKELKIDSPVWITGIGYANDYAYVARRGEWVGFKATQLAARQAYEMAKVTPNDIEVATVHDAFTIAEIMGYEDLGFTEKGKGGKFIEEGQSEKGGKVGVNLFGGLKAKGHPLGATGLSMIYEITKQLRDEADKLQQPLKKYIGLVHNVGGTGHFAYVMILRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VN7</accession>
    <name>Q97VN7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH asnC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2581</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90431">
        <property type="entry name" value="C90431"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989290.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VN7"/>
    <dbReference type="SMR" id="Q97VN7"/>
    <dbReference type="STRING" id="273057.SSO2581"/>
    <dbReference type="PaxDb" id="273057-SSO2581"/>
    <dbReference type="EnsemblBacteria" id="AAK42706">
        <property type="protein sequence ID" value="AAK42706"/>
        <property type="gene ID" value="SSO2581"/>
    </dbReference>
    <dbReference type="GeneID" id="1454033"/>
    <dbReference type="KEGG" id="sso:SSO2581"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2663"/>
    <dbReference type="eggNOG" id="arCOG01586">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090242_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VN7"/>
    <dbReference type="PhylomeDB" id="Q97VN7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF8">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR PTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="255" mass="29740" checksum="598F4639B88BA510" modified="2001-10-01" version="1">MDSIDKGILKILLKDARTPQRKIAIMLGISPPAVSYRMEKLFGDIIKRFTVYVNPNFLGRYHGYVAFNNLYDWDGDYIAKIECLEEINVYEIDSKSRVELDEKIRKMSEKLGEPRMIYIPNQMPYSPSKFDLKVVSILKEKPLIKPIELAEELGVSSKTIRRHLRYLYSKKFIRLIPIIDLNKAEISIFAVFTSKVEDGKNFFAKYAFTEIEDDKAGIFVNVVDSMDEARDLALKFKREYDKDAEIMITTKYEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VP2</accession>
    <name>Q97VP2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc-containing ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">zfx-1</name>
        <name evidence="13" type="ordered locus">SSO2574</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10 11">Binds 1 zinc ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="10 11">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10 11">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90430">
        <property type="entry name" value="F90430"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991787.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VP2"/>
    <dbReference type="STRING" id="273057.SSO2574"/>
    <dbReference type="PaxDb" id="273057-SSO2574"/>
    <dbReference type="EnsemblBacteria" id="AAK42701">
        <property type="protein sequence ID" value="AAK42701"/>
        <property type="gene ID" value="SSO2574"/>
    </dbReference>
    <dbReference type="GeneID" id="1454028"/>
    <dbReference type="KEGG" id="sso:SSO2574"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2657"/>
    <dbReference type="eggNOG" id="arCOG04548">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178207_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VP2"/>
    <dbReference type="PhylomeDB" id="Q97VP2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009157">
        <property type="entry name" value="Fd_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF6">
        <property type="entry name" value="L-ASPARTATE SEMIALDEHYDE SULFURTRANSFERASE IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000068">
        <property type="entry name" value="Zn_Fdx_Sulfol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 10 11" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="2 10 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 10" id="KW-0813">Transport</keyword>
    <keyword evidence="5 10 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="36"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="75"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000068-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAK42701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="104" mass="11044" checksum="191E6DBB11725573" modified="2001-10-01" version="1">MGIDPNYRTNFPVSGEHSGHKVYGTVEPPGKLGIHGTIVGVDFDLCIADGSCINACPVNVFQWYDTPGHPASEKKADPVNEQACIFCMACVNVCPVAAIDVKPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VS7</accession>
    <name>Q97VS7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cellulase (Endo 1,4 beta glucanase), putative (CelB)</fullName>
            <ecNumber evidence="2">3.2.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">celB</name>
        <name evidence="2" type="ordered locus">SSO2534</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 12 (cellulase H) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90425">
        <property type="entry name" value="H90425"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990980.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VS7"/>
    <dbReference type="SMR" id="Q97VS7"/>
    <dbReference type="STRING" id="273057.SSO2534"/>
    <dbReference type="CAZy" id="GH12">
        <property type="family name" value="Glycoside Hydrolase Family 12"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2534"/>
    <dbReference type="EnsemblBacteria" id="AAK42663">
        <property type="protein sequence ID" value="AAK42663"/>
        <property type="gene ID" value="SSO2534"/>
    </dbReference>
    <dbReference type="GeneID" id="1453990"/>
    <dbReference type="KEGG" id="sso:SSO2534"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2610"/>
    <dbReference type="eggNOG" id="arCOG03858">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_865009_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VS7"/>
    <dbReference type="PhylomeDB" id="Q97VS7"/>
    <dbReference type="BRENDA" id="3.2.1.4">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013319">
        <property type="entry name" value="GH11/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002594">
        <property type="entry name" value="GH12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01670">
        <property type="entry name" value="Glyco_hydro_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="322" mass="36710" checksum="6C32D7765173A768" modified="2001-10-01" version="1">MNKLIPIFVVVIIVLGIIVSIEFGKFHQNASLTRSTERFTLFPAHNRPFSVLGNYSSNSADALAILNSSTNATLMVSPFLWNIGYALGNVNMTININYLHVAINLSQISKISSNVVDGYPGLMYGQELWWPFMYRTTQLQFLSLPMIVLRLPNFYSILNYSVYLINGSIDDFSYDIWLSQNPNITSLQYGDFEIMIWMYWNENLSHTPYFIYVGNMSIPTLINGKIENLSWEVYVLPRTGSANGWTGVYFLSPLKEPKAEFGVPIGYILKNMGSYIEKAGVNIYNVNTYYLDAIQVGMEFSDNQGTAIMGYYLYSWQIWLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VU0</accession>
    <name>Q97VU0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cyclase/dehydrase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2519</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90424">
        <property type="entry name" value="C90424"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988480.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VU0"/>
    <dbReference type="STRING" id="273057.SSO2519"/>
    <dbReference type="PaxDb" id="273057-SSO2519"/>
    <dbReference type="EnsemblBacteria" id="AAK42650">
        <property type="protein sequence ID" value="AAK42650"/>
        <property type="gene ID" value="SSO2519"/>
    </dbReference>
    <dbReference type="GeneID" id="1453977"/>
    <dbReference type="KEGG" id="sso:SSO2519"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2596"/>
    <dbReference type="eggNOG" id="arCOG00507">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056342_1_1_2"/>
    <dbReference type="InParanoid" id="Q97VU0"/>
    <dbReference type="PhylomeDB" id="Q97VU0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd16282">
        <property type="entry name" value="metallo-hydrolase-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF18">
        <property type="entry name" value="METALLO-HYDROLASE MJ0296-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="26"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="308" mass="35615" checksum="6DB2EA23DA00495C" modified="2001-10-01" version="1">MEVQYKFERISDNVYAFIQPNGDWFLSNAGVIIGKNYAVVVDSLTNEKMARQFISEIKKITDKPIKYLINTHEHEDHLWTNHLFPNAITICHKNCREKVVEGAKRRINPYENLFNAIDFSGYQYTPQEVVIKDEMRIFIDNDEEIKIAYVGYAHTTSDIYVYIPDQKVVFTGDLLFSPPCTPFALMGYIQGYINALENLANLDAEIFIPGHGEVSYGRNALYESRDYLIFVRDEARKIMRTISDPIRAAYEINLGKYDEWISKERIVGNLVRAYSELKGNPPASKLENIDKVLLEMIRYREKKGGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VU1</accession>
    <name>Q97VU1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Esterase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2518</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2007-09" db="PDB data bank">
            <title>Crystal structure of a putative lipase (NP_343859.1) from Sulfolobus solfataricus at 1.85 A resolution.</title>
            <authorList>
                <consortium name="Joint Center for Structural Genomics (JCSG)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH CA(2+)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90424">
        <property type="entry name" value="B90424"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988483.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2RAU">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.85 A"/>
        <property type="chains" value="A=1-353"/>
    </dbReference>
    <dbReference type="PDBsum" id="2RAU"/>
    <dbReference type="AlphaFoldDB" id="Q97VU1"/>
    <dbReference type="SMR" id="Q97VU1"/>
    <dbReference type="STRING" id="273057.SSO2518"/>
    <dbReference type="ESTHER" id="sulso-SSO2518">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2518"/>
    <dbReference type="DNASU" id="1453976"/>
    <dbReference type="EnsemblBacteria" id="AAK42649">
        <property type="protein sequence ID" value="AAK42649"/>
        <property type="gene ID" value="SSO2518"/>
    </dbReference>
    <dbReference type="GeneID" id="1453976"/>
    <dbReference type="KEGG" id="sso:SSO2518"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2595"/>
    <dbReference type="eggNOG" id="arCOG07204">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_707170_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VU1"/>
    <dbReference type="BRENDA" id="3.1.1.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97VU1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="72"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2RAU"/>
        </source>
    </evidence>
    <sequence length="353" mass="40450" checksum="9CF97FE284914D25" modified="2001-10-01" version="1">MYEEWKIVKREAPILGNDQLIENIWKMKREDSPYDIISLHKVNLIGGGNDAVLILPGTWSSGEQLVTISWNGVHYTIPDYRKSIVLYLARNGFNVYTIDYRTHYVPPFLKDRQLSFTANWGWSTWISDIKEVVSFIKRDSGQERIYLAGESFGGIAALNYSSLYWKNDIKGLILLDGGPTKHGIRPKFYTPEVNSIEEMEAKGIYVIPSRGGPNNPIWSYALANPDMPSPDPKYKSISDFLMDSLYVTGSANPYDYPYSKKEDMFPILASFDPYWPYRLSLERDLKFDYEGILVPTIAFVSERFGIQIFDSKILPSNSEIILLKGYGHLDVYTGENSEKDVNSVVLKWLSQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VU2</accession>
    <name>Q97VU2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carboxylesterase (Est)</fullName>
            <ecNumber evidence="3">3.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">est</name>
        <name evidence="3" type="ordered locus">SSO2517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90424">
        <property type="entry name" value="A90424"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988484.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VU2"/>
    <dbReference type="SMR" id="Q97VU2"/>
    <dbReference type="FunCoup" id="Q97VU2">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2517"/>
    <dbReference type="ESTHER" id="sulso-est">
        <property type="family name" value="Hormone-sensitive_lipase_like"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2517"/>
    <dbReference type="EnsemblBacteria" id="AAK42648">
        <property type="protein sequence ID" value="AAK42648"/>
        <property type="gene ID" value="SSO2517"/>
    </dbReference>
    <dbReference type="GeneID" id="1453975"/>
    <dbReference type="KEGG" id="sso:SSO2517"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2594"/>
    <dbReference type="eggNOG" id="arCOG02638">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_6_2_2"/>
    <dbReference type="InParanoid" id="Q97VU2"/>
    <dbReference type="PhylomeDB" id="Q97VU2"/>
    <dbReference type="BRENDA" id="3.1.1.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="3.1.1.79">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF8">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="21"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="251" mass="27811" checksum="EEDFFE1E82070AFB" modified="2001-10-01" version="1">METEIPIRIYYPDNKRDHPIILHFHGGAWILGSIETEDSVSRILANSCNCIVISVNYRLAPEHKFPAAVTDCFDSIKWTYENAESIGGHPNRIAVFGISAGGNLAAATSILSRDQGIKLRAQALVVPFVYLDLASTSMTEYRKGYFLDINVPIDYGIMMYIRDEKDLLNPMFVPLIAEDLSNLPQAIIVTAEYDPLRDQGEAYAKRLMEAGVLTLSFRVNGMVHGFLGSPNISRLVSVMVGSALKDILMRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VX0</accession>
    <name>Q97VX0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">DNA endonuclease III (NtH-2)</fullName>
            <ecNumber evidence="12">4.2.99.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ntH-2</name>
        <name evidence="12" type="ordered locus">SSO2484</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="12"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90420">
        <property type="entry name" value="E90420"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923888.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VX0"/>
    <dbReference type="FunCoup" id="Q97VX0">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2484"/>
    <dbReference type="PaxDb" id="273057-SSO2484"/>
    <dbReference type="EnsemblBacteria" id="AAK42620">
        <property type="protein sequence ID" value="AAK42620"/>
        <property type="gene ID" value="SSO2484"/>
    </dbReference>
    <dbReference type="GeneID" id="7808239"/>
    <dbReference type="KEGG" id="sso:SSO2484"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2565"/>
    <dbReference type="eggNOG" id="arCOG00461">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_6_0_2"/>
    <dbReference type="InParanoid" id="Q97VX0"/>
    <dbReference type="PhylomeDB" id="Q97VX0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004035">
        <property type="entry name" value="Endouclease-III_FeS-bd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359">
        <property type="entry name" value="A/G-SPECIFIC ADENINE GLYCOSYLASE/ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359:SF19">
        <property type="entry name" value="DNA REPAIR GLYCOSYLASE MJ1434-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001435">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00764">
        <property type="entry name" value="ENDONUCLEASE_III_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="11">
        <location>
            <begin position="51"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK42620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="227" mass="26344" checksum="0A53EE8BD8684EE8" modified="2001-10-01" version="1">MVRKILDTLLKIFENNKSILKEKGWIVSSETSYEWWDGLKSAEEIIISAILVQMSRWEIVKSKVEEMRSKGLTDFYKLCNTTEQELYDVLKGINFYKTKVKRLINLSKIIINLGSVEKFYNRNLLLSIDGIGEETADSILLFAGHKPNFPPSEYGKRVLSRVLGISIKKKNEVKRLVEENLERNVYEYKLLHAGIVTVGRAFCFIENPKCEDCILKKVCKYYRENSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W28</accession>
    <name>Q97W28_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dihydropteroate synthase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2415</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90413">
        <property type="entry name" value="C90413"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989412.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W28"/>
    <dbReference type="STRING" id="273057.SSO2415"/>
    <dbReference type="PaxDb" id="273057-SSO2415"/>
    <dbReference type="EnsemblBacteria" id="AAK42562">
        <property type="protein sequence ID" value="AAK42562"/>
        <property type="gene ID" value="SSO2415"/>
    </dbReference>
    <dbReference type="GeneID" id="1453889"/>
    <dbReference type="KEGG" id="sso:SSO2415"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2497"/>
    <dbReference type="eggNOG" id="arCOG01978">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041129_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W28"/>
    <dbReference type="PhylomeDB" id="Q97W28"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042558">
        <property type="term" value="P:pteridine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005236">
        <property type="entry name" value="Dihydropt_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045406">
        <property type="entry name" value="DUF6513"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025595">
        <property type="entry name" value="PterinBD-DUF4346"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00284">
        <property type="entry name" value="dihydropteroate synthase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20123">
        <property type="entry name" value="DUF6513"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14251">
        <property type="entry name" value="PterinBD-DUF4346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pterin-binding" evidence="2">
        <location>
            <begin position="96"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="498" mass="56175" checksum="0A55A6523CF73C8D" modified="2001-10-01" version="1">MKVLVVTGTLAAPILSEVAKNIKDTKVEIKVLNYPVASLMSTKFIAENLKQTKFDADYILLPGLVYGDAKIVEEVTGVRTFKGTEEAWDLPRVIEALKNGIQLSTTESADKIIGKMDNIEEKLRKMEEEAKISFEINGIKITTYPPPFRIFLEIDNKQEFEKLDRIRKNVDVVVLGFPVGHYDLDEVKNKVKQLVDYGYVVGIDAESPRELKEGVRAGASFVFNLNENNFEELEEIRKEAAFVVAPFNTENRGEITIDLVKKAKQKGFDKLIADPVLSPPLRGLVSSIIGYKYVRETLQDIPILMGILNVTELIDADSIGMNALLTAIAGELGISNLLIMEKGKTRWSSWEVSQATKMISVALKENRLPKDIGIDLLVLKDKRRFRESFNADVIVNRRIEPEMDNTGFAKIFVSEDGFGVEWIGKNKITIKGKDGLSIGRELIRRVKDISKEHAVYIGYELAKAEIAYQLDKNYIQDKPLFKKIINDNLHTEHDKKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W30</accession>
    <name>Q97W30_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">6-pyruvoyl tetrahydrobiopterin synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2412</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90413">
        <property type="entry name" value="A90413"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989418.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W30"/>
    <dbReference type="STRING" id="273057.SSO2412"/>
    <dbReference type="PaxDb" id="273057-SSO2412"/>
    <dbReference type="EnsemblBacteria" id="AAK42560">
        <property type="protein sequence ID" value="AAK42560"/>
        <property type="gene ID" value="SSO2412"/>
    </dbReference>
    <dbReference type="GeneID" id="1453887"/>
    <dbReference type="KEGG" id="sso:SSO2412"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2495"/>
    <dbReference type="eggNOG" id="arCOG02172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111016_6_0_2"/>
    <dbReference type="InParanoid" id="Q97W30"/>
    <dbReference type="PhylomeDB" id="Q97W30"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="entry name" value="6-pyruvoyl tetrahydropterin synthase/QueD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589:SF7">
        <property type="entry name" value="6-PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15972" checksum="F4A0502EBED836FA" modified="2001-10-01" version="1">MKVRVGIEGITMDSAHYTLSSYADSQIHGHTYIVNVEVEGEVNEKSGFVVDFNLLKKIIKETIQEWDHKLIIPKVDLDKSRFEGPFRVDYKVIDAPFPTAEYIGIEIAKDIYLKLNKKYRILLKIYEGKDSYAIIEYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W91</accession>
    <name>Q97W91_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ribosomal protein S6 modification protein (RimK-3)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rimK-3</name>
        <name evidence="6" type="ordered locus">SSO2346</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90405">
        <property type="entry name" value="B90405"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W91"/>
    <dbReference type="FunCoup" id="Q97W91">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2346"/>
    <dbReference type="PaxDb" id="273057-SSO2346"/>
    <dbReference type="EnsemblBacteria" id="AAK42497">
        <property type="protein sequence ID" value="AAK42497"/>
        <property type="gene ID" value="SSO2346"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2346"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2430"/>
    <dbReference type="eggNOG" id="arCOG01589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054353_2_0_2"/>
    <dbReference type="InParanoid" id="Q97W91"/>
    <dbReference type="PhylomeDB" id="Q97W91"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="Rp_bS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF0">
        <property type="entry name" value="BETA-CITRYLGLUTAMATE SYNTHASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="5">
        <location>
            <begin position="44"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="230" mass="25647" checksum="D48D24FB80BAFCB2" modified="2001-10-01" version="1">MGFISTTEQFIKRFDVLRELERNGVVLMNRPDSMLLARDKFASLMRMRRAGIPVPNTALVEDPFEVMRLAERWGEVVIKPVVGSLGLGSVKVSDPDIAFRVAKAILSVNQPVYVQKYVKKPDRDIRVIVIGDKVLGSIYRISKSGWKTNIAQGATAQVLIPDAELEEISLKSVRVLGLDYAGIDIIEDVENGGYKIIEVNAAPLWDGFEAATNINPAKYIVTHLIEKIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WB8</accession>
    <name>Q97WB8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Flagella-related protein J, putative (FlaJ)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FlaJ</name>
        <name evidence="8" type="ordered locus">SSO2315</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90401">
        <property type="entry name" value="G90401"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992490.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WB8"/>
    <dbReference type="STRING" id="273057.SSO2315"/>
    <dbReference type="PaxDb" id="273057-SSO2315"/>
    <dbReference type="EnsemblBacteria" id="AAK42470">
        <property type="protein sequence ID" value="AAK42470"/>
        <property type="gene ID" value="SSO2315"/>
    </dbReference>
    <dbReference type="GeneID" id="1453797"/>
    <dbReference type="KEGG" id="sso:SSO2315"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2404"/>
    <dbReference type="eggNOG" id="arCOG01809">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_574427_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WB8"/>
    <dbReference type="PhylomeDB" id="Q97WB8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056569">
        <property type="entry name" value="ArlJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402:SF2">
        <property type="entry name" value="FLAGELLA ACCESSORY PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="20"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="473" mass="53338" checksum="0601E37ECE6F995D" modified="2001-10-01" version="1">MLRIFSAKNEVDSKYIFMLAFMLALFSSGVPPEIVILHLSREDSFEPYTKVFKKIRNLVSGYRYKFSSAITYSIKNLNIRFLKEFLIRLSQAVTFGDDMIEFLSREIDFTLSEYNAYSARLIESMNNFLTIYATLNSSLTFLVADITILSLIYSGGTTLIIQLTILSLALLGNLTLVMYLLYRPENYIRYNVIDKFVLTFTISLSIIFSVIYTSYIVLMGTGIVLLGIGLRYRLYENKINRIERHFLLFIRYFSRNYAIVSNLKESLMAVLRGDLGDVRPLIRRSLNRLNFGIDKEKIFRLIGDESGSILVSMLSKVLYETISMGGNAMIIGEILSKIGDSVLNIRSRKEQNGRAFETSIYALQTASAGVSAALISIVGMLNNIFSTQNVSTVFSFSEVNIDIISRIFLIILLILSFANGIAITLAYGKSLYVSLYFIGILIILSSISYHIVLILTGNIFKELASPSGLLQTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WD8</accession>
    <name>Q97WD8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2288</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90399">
        <property type="entry name" value="C90399"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WD8"/>
    <dbReference type="STRING" id="273057.SSO2288"/>
    <dbReference type="PaxDb" id="273057-SSO2288"/>
    <dbReference type="EnsemblBacteria" id="AAK42450">
        <property type="protein sequence ID" value="AAK42450"/>
        <property type="gene ID" value="SSO2288"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2288"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2381"/>
    <dbReference type="eggNOG" id="arCOG00131">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_743178_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WD8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="341"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="41921" checksum="4D9C450088BC250F" modified="2001-10-01" version="1">MIRKYIGQYFIITSFTMIGLLFPVEFYSETKSLILLGVITGIYNALNALGSYIWGYLIDKTRLRKEYAVLLSLFGIIIGLTYHYNKLIAYELSGFVSALDGPIYSAILLETTPQEKLVLGNTRLSQISLAGNVTGSLLSAFYHNDYLILIFFSISLAFNMIHIPKYDGGINYDVADRNKILRILFIPIISYFWFNMAAEIFYTLYVPLNYLMLNPSYIIFISYTFLYIIEELIYSKGINIVKGKEEYFMLLVTFARSLIVMSIVYIILMGLKIYEGTMLLFLVFGPLFPIYNISFFSLLIKGLKRNKATIIGIFNVSEDIANVVGGFLSGSTNNIVSSYQISFYSFALSLFLLYFYLRRPRRVSASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WF6</accession>
    <name>Q97WF6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2268</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90396">
        <property type="entry name" value="H90396"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WF6"/>
    <dbReference type="PaxDb" id="273057-SSO2268"/>
    <dbReference type="EnsemblBacteria" id="AAK42431">
        <property type="protein sequence ID" value="AAK42431"/>
        <property type="gene ID" value="SSO2268"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2268"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2360"/>
    <dbReference type="eggNOG" id="arCOG08373">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582967_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WF6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="186" mass="21460" checksum="B2930508B4A6D2C4" modified="2001-10-01" version="1">MNRYIYHVDIVVNVKQYLMVIAGVLVSLAIASLVVYISHMFIIYNIKKPNIVERQSGYFPEMYELTVNITPAKASYANSYLIWSPLQSSLVVNKDPIFHVYPSIIDLNSFNNSVIITLYYSVQDRVNIAITQQIFNVSVVNTTVINDEIVATQLKVTFYNASPNTIYLIPIWNQYNVTYYVLVYYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WF9</accession>
    <name>Q97WF9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2250 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2263</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90396">
        <property type="entry name" value="E90396"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991526.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WF9"/>
    <dbReference type="STRING" id="273057.SSO2263"/>
    <dbReference type="PaxDb" id="273057-SSO2263"/>
    <dbReference type="EnsemblBacteria" id="AAK42428">
        <property type="protein sequence ID" value="AAK42428"/>
        <property type="gene ID" value="SSO2263"/>
    </dbReference>
    <dbReference type="GeneID" id="1453755"/>
    <dbReference type="KEGG" id="sso:SSO2263"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2355"/>
    <dbReference type="eggNOG" id="arCOG03698">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1324070_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WF9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019254">
        <property type="entry name" value="DUF2250"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017139">
        <property type="entry name" value="UCP037213"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10007">
        <property type="entry name" value="DUF2250"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037213">
        <property type="entry name" value="UCP037213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="24962" checksum="61C90D88E832454B" modified="2001-10-01" version="1">MSDREELSAKLRIILRDIRYLTVLKHLKIANVDYGKSIMLNTKIPLTEIVEILDDLEKLGLIERVHGATLKNTEAKFKLSSEVHKHHTYYRLTREGDHLLRHLDDRMLIDVYMDIVKNDNLALNILRLADELNADHALTYAKLTHKRLEEITPKIEELVKMGLLEEKNSKIIKFADRKAKPKKETRTHHKYYGLSRIGELVVREMKRRGILPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WG0</accession>
    <name>Q97WG0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2262</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AB hydrolase superfamily. ABHD14 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90396">
        <property type="entry name" value="D90396"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991527.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WG0"/>
    <dbReference type="FunCoup" id="Q97WG0">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2262"/>
    <dbReference type="ESTHER" id="sulso-SSO2262">
        <property type="family name" value="CIB-CCG1-interacting-factor-B"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2262"/>
    <dbReference type="EnsemblBacteria" id="AAK42427">
        <property type="protein sequence ID" value="AAK42427"/>
        <property type="gene ID" value="SSO2262"/>
    </dbReference>
    <dbReference type="GeneID" id="1453754"/>
    <dbReference type="KEGG" id="sso:SSO2262"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2354"/>
    <dbReference type="eggNOG" id="arCOG01648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_28_1_2"/>
    <dbReference type="InParanoid" id="Q97WG0"/>
    <dbReference type="PhylomeDB" id="Q97WG0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46197:SF3">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46197">
        <property type="entry name" value="PROTEIN ABHD14B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="26"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="197" mass="21727" checksum="F65228BEE08836AB" modified="2001-10-01" version="1">MQQLIDKFINVKGRKIHYIESGDGSLVLLFHGARFNARTWVETNTVDSISSIGYKAISVDLPGFGSSEKIEGISLSEFINLFMNSLGIDKAILLGASMGGKAVLEFSLKYTESVSGLVLVGAVGVEEFEVQLSKLNKIPLLLIWGSRDTVSPKRNYKLILDKVKNAKLEIVGKNHACYLDDPNTFNEKIVNFLKGMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WL5</accession>
    <name>Q97WL5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5591 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2201</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90389">
        <property type="entry name" value="D90389"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992091.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WL5"/>
    <dbReference type="STRING" id="273057.SSO2201"/>
    <dbReference type="PaxDb" id="273057-SSO2201"/>
    <dbReference type="EnsemblBacteria" id="AAK42371">
        <property type="protein sequence ID" value="AAK42371"/>
        <property type="gene ID" value="SSO2201"/>
    </dbReference>
    <dbReference type="GeneID" id="1453698"/>
    <dbReference type="KEGG" id="sso:SSO2201"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2297"/>
    <dbReference type="eggNOG" id="arCOG00995">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1536567_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WL5"/>
    <dbReference type="PhylomeDB" id="Q97WL5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10630">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040777">
        <property type="entry name" value="DUF5591"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17884">
        <property type="entry name" value="DUF5591"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUF5591" evidence="2">
        <location>
            <begin position="34"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="196" mass="23015" checksum="C82E29D0031B4ECA" modified="2001-10-01" version="1">MSSACEQLYEYLKPDFTRISKRDNTDDLFRHPVVMRWHRYFLENWSSNKEIALLLPCTVVKPYSSSPTHKIAYAILQKYNLEEKVQVYSVSEPMLLVPRELEECYPFNNYDYPPALMSKEEKEEFVELLTKPLIKISKLHERLIGILPRHHYEIVKGAAEISHLKITIYPYGRLAFKTIANVIRNLSLSNPRSLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WT3</accession>
    <name>Q97WT3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cobalt transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2031</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90370">
        <property type="entry name" value="C90370"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923739.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WT3"/>
    <dbReference type="FunCoup" id="Q97WT3">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2031"/>
    <dbReference type="PaxDb" id="273057-SSO2031"/>
    <dbReference type="EnsemblBacteria" id="AAK42218">
        <property type="protein sequence ID" value="AAK42218"/>
        <property type="gene ID" value="SSO2031"/>
    </dbReference>
    <dbReference type="GeneID" id="7797418"/>
    <dbReference type="KEGG" id="sso:SSO2031"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2112"/>
    <dbReference type="eggNOG" id="arCOG02250">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056469_2_0_2"/>
    <dbReference type="InParanoid" id="Q97WT3"/>
    <dbReference type="PhylomeDB" id="Q97WT3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16914">
        <property type="entry name" value="EcfT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33514">
        <property type="entry name" value="PROTEIN ABCI12, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33514:SF13">
        <property type="entry name" value="PROTEIN ABCI12, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="35772" checksum="39E9E23568151175" modified="2001-10-01" version="1">MNTLVDQIISWIIVLYGSAVPALFVIFLIGITGLREITRYETGNTFLYKLNPVTKVVLTIVVMTVAATTIWWIGAILTLVLLLSYLTLNDGVRKFGYAFFLALSTILGGTWSIAPYTPPSTLQLAFPSVKQFTVIWTFPSYFQVMGYEQQLTLEALIYGLQTTFRVAPVLLSALLFITTTTGSDIFRMFTKIKIPIAITFSVLVGIRMIPRIFELLDTSVKMQYIRGLGYSRPKIVSSLLIVYAIFEAIIPTMVYLFRGAKNLAISADTRGFRAYPSRTSLVELKFTRVDYYGTAIIAALIIIAIVANLLGFGRTIPYVGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WX0</accession>
    <name>CMR2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR system CMR subunit Cmr2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>CRISPR-associated protein Cas10/Cmr2, subtype III-B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cmr2</name>
        <name type="ordered locus">SSO1991</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="Mol. Cell" volume="45" first="303" last="313">
            <title>Structure and mechanism of the CMR complex for CRISPR-mediated antiviral immunity.</title>
            <authorList>
                <person name="Zhang J."/>
                <person name="Rouillon C."/>
                <person name="Kerou M."/>
                <person name="Reeks J."/>
                <person name="Brugger K."/>
                <person name="Graham S."/>
                <person name="Reimann J."/>
                <person name="Cannone G."/>
                <person name="Liu H."/>
                <person name="Albers S.V."/>
                <person name="Naismith J.H."/>
                <person name="Spagnolo L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="22227115"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2011.12.013"/>
        </citation>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION IN CMR COMPLEX</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). The CMR complex degrades RNA complementary to the crRNA (target RNA) within UA dinucleotides, generating 3'-OH and 5'-phosphate ends. Activity is dependent on the 8 nt long 5' tag in the crRNA, an unpaired 3' flag on the target RNA, and is stimulated by ATP. Some cleavage of the guide crRNA can also be observed.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="1">Binds 2 Ca(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 Zn(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the CMR ribonucleoprotein complex, consisting of crRNA plus Cmr1/Cmr2/Cmr3/Cmr4/Cmr5/Cmr6 at 1:1 and possibly 3 Cmr7 dimers. A Cmr2/Cmr3/Cmr7 subcomplex without crRNA can also be isolated. It does not cleave target RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR system Cmr2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90365">
        <property type="entry name" value="F90365"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992992.1">
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        <property type="gene ID" value="SSO1991"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:defense response to virus"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="CRISPR-Cas system, Cmr2 subunit, D1 domain, cysteine cluster"/>
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        <property type="entry name" value="Cas10-Cmr2_palm2"/>
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        <property type="entry name" value="Cmr2_N"/>
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        <property type="entry name" value="CRISPR-assoc_Cmr2_N"/>
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    <keyword id="KW-0862">Zinc</keyword>
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            <begin position="1"/>
            <end position="1045"/>
        </location>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>ADP</name>
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        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="514"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="517"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="652"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="810"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="811"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="811"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="22227115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="1045" mass="121641" checksum="2F8DCACF254DF968" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X34</accession>
    <name>Q97X34_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X34"/>
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    <dbReference type="EnsemblBacteria" id="AAK42114">
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    <dbReference type="KEGG" id="sso:SSO1920"/>
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        <source>
            <dbReference type="EMBL" id="AAK42114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="103" mass="12118" checksum="8FF5DE48B5501B3B" modified="2001-10-01" version="1">MQRAYTFLKTKYRKTLGKAYIIVKLNLIIDNKQNRRKFLPTSIFLRVLKHIVKLVNPITSVITLKNQIIEFILNKLQNIIQVIISINPTLQILPNSIRFKSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X54</accession>
    <name>Q97X54_SACS2</name>
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        <recommendedName>
            <fullName evidence="1">Methyltransferase FkbM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1901</name>
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    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="AAK42092">
        <property type="protein sequence ID" value="AAK42092"/>
        <property type="gene ID" value="SSO1901"/>
    </dbReference>
    <dbReference type="GeneID" id="71027452"/>
    <dbReference type="KEGG" id="sso:SSO1901"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1959"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_968463_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
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        <property type="entry name" value="SAM-dependent_MTase"/>
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    </dbReference>
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        <property type="entry name" value="SLL1173 PROTEIN"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="103"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="266" mass="30889" checksum="9B7B0346DC3051CA" modified="2001-10-01" version="1">MRIEELKPKPPSLKEYLKVKPLGFSSGLFLYYIYLKTYINLRYFNRFPKINEGYTKLLKVKYDGMYWYVRKENLVHDIWIILLHNEPEVRKWISFEKDMIFVDVGAYIGSYTIRAGIRGANVIAFEPNPVSFKILELNVKENEIESKVKLFNNAVWSRSGKIELFANNDMTSAYNNKGNKIVVNAIALDSLNLKRVNLLKVDVEGAELEVLKGAMNTLDVTDKILIEVRKEFERNIDDLLRSKGFKLSKVDTTYDSIGNFLYEKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X55</accession>
    <name>Q97X55_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1900</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90354">
        <property type="entry name" value="D90354"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992022.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X55"/>
    <dbReference type="SMR" id="Q97X55"/>
    <dbReference type="FunCoup" id="Q97X55">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1900"/>
    <dbReference type="PaxDb" id="273057-SSO1900"/>
    <dbReference type="EnsemblBacteria" id="AAK42091">
        <property type="protein sequence ID" value="AAK42091"/>
        <property type="gene ID" value="SSO1900"/>
    </dbReference>
    <dbReference type="GeneID" id="1453418"/>
    <dbReference type="KEGG" id="sso:SSO1900"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1958"/>
    <dbReference type="eggNOG" id="arCOG00615">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_1_2_2"/>
    <dbReference type="InParanoid" id="Q97X55"/>
    <dbReference type="PhylomeDB" id="Q97X55"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02803">
        <property type="entry name" value="OYE_like_FMN_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044152">
        <property type="entry name" value="YqjM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303:SF4">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="6">
        <location>
            <begin position="6"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="555" mass="62811" checksum="A9CC87ED01D4D172" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X58</accession>
    <name>Q97X58_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-haloalkanoic acid dehalogenase</fullName>
            <ecNumber evidence="2">3.8.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.8.1.2" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90353">
        <property type="entry name" value="G90353"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992764.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X58"/>
    <dbReference type="FunCoup" id="Q97X58">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1896"/>
    <dbReference type="PaxDb" id="273057-SSO1896"/>
    <dbReference type="EnsemblBacteria" id="AAK42086">
        <property type="protein sequence ID" value="AAK42086"/>
        <property type="gene ID" value="SSO1896"/>
    </dbReference>
    <dbReference type="GeneID" id="1453413"/>
    <dbReference type="KEGG" id="sso:SSO1896"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1951"/>
    <dbReference type="eggNOG" id="arCOG02291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_8_0_2"/>
    <dbReference type="InParanoid" id="Q97X58"/>
    <dbReference type="PhylomeDB" id="Q97X58"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018784">
        <property type="term" value="F:(S)-2-haloacid dehalogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051828">
        <property type="entry name" value="HAD-like_hydrolase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191:SF2">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="212" mass="24493" checksum="D3A7DD86E1065051" modified="2001-10-01" version="1">MYRAIFLDFGNTLVGFKPAFYEKLQIILKEHGYDVEIRKVFRAYVKAMAINNYAQPTDIKEFLYNLSIPPDEKLINSIMSSDIRDGEAFVYDEVIEFLETIRSANLKLILVSNSSPRTKKLLEELGLVKYFDNLVLSHEIGIVKPNPKIFAIAISKGGYPALHIGDIYEIDYIGARRSYLDAVLLDRYDFYPEIKEKVRDLREIIPTIMKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X70</accession>
    <name>Q97X70_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Partial ORF from ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO9134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99352">
        <property type="entry name" value="A99352"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X70"/>
    <dbReference type="PaxDb" id="273057-SSO9134"/>
    <dbReference type="EnsemblBacteria" id="AAK42072">
        <property type="protein sequence ID" value="AAK42072"/>
        <property type="gene ID" value="SSO9134"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO9134"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1937"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2802454_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X70"/>
    <dbReference type="PhylomeDB" id="Q97X70"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="67" mass="7702" checksum="7CD40ABCEEB51F8E" modified="2001-10-01" version="1">MILNNEVSRVIIAHPDRLVRFGLEILKEVSKVHNYEIVALNKEDKTSEELVEDLISSANGEERDERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X88</accession>
    <name>Q97X88_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF488 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1867</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90349">
        <property type="entry name" value="G90349"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988464.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X88"/>
    <dbReference type="STRING" id="273057.SSO1867"/>
    <dbReference type="PaxDb" id="273057-SSO1867"/>
    <dbReference type="EnsemblBacteria" id="AAK42054">
        <property type="protein sequence ID" value="AAK42054"/>
        <property type="gene ID" value="SSO1867"/>
    </dbReference>
    <dbReference type="GeneID" id="1453381"/>
    <dbReference type="KEGG" id="sso:SSO1867"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1917"/>
    <dbReference type="eggNOG" id="arCOG06027">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137928_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X88"/>
    <dbReference type="PhylomeDB" id="Q97X88"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052552">
        <property type="entry name" value="YeaO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36849:SF1">
        <property type="entry name" value="CYTOPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36849">
        <property type="entry name" value="CYTOPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22752">
        <property type="entry name" value="DUF488-N3i"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="13663" checksum="2CB7423B6028B6F8" modified="2001-10-01" version="1">MIKLKRVYDPIERDDGIRILVDRLWPRGIGKDKVDLWLKDIAPSDELRKWFSHDTSKWDEFKKKYFEELDANPKISVLLQLIKKGENITLLYASKSPYNNAVALKEYLEKKILKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-15" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X93</accession>
    <name>Y1861_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0273 protein SSO1861</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO1861</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0273 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90349">
        <property type="entry name" value="B90349"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988518.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X93"/>
    <dbReference type="SMR" id="Q97X93"/>
    <dbReference type="STRING" id="273057.SSO1861"/>
    <dbReference type="PaxDb" id="273057-SSO1861"/>
    <dbReference type="EnsemblBacteria" id="AAK42049">
        <property type="protein sequence ID" value="AAK42049"/>
        <property type="gene ID" value="SSO1861"/>
    </dbReference>
    <dbReference type="GeneID" id="1453376"/>
    <dbReference type="KEGG" id="sso:SSO1861"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1911"/>
    <dbReference type="eggNOG" id="arCOG01171">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023669_2_0_2"/>
    <dbReference type="InParanoid" id="Q97X93"/>
    <dbReference type="PhylomeDB" id="Q97X93"/>
    <dbReference type="BRENDA" id="3.6.4.B4">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19486">
        <property type="entry name" value="KaiC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01076">
        <property type="entry name" value="UPF0273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022475">
        <property type="entry name" value="UPF0273_KaiC-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03877">
        <property type="entry name" value="thermo_KaiC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF1">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01874">
        <property type="entry name" value="DNAREPAIRADA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184592" description="UPF0273 protein SSO1861">
        <location>
            <begin position="1"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="KaiC" evidence="1">
        <location>
            <begin position="2"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01076"/>
        </source>
    </evidence>
    <sequence length="280" mass="31337" checksum="AB554C0595306FA5" modified="2001-10-01" version="1">MKRVKTYIPGLDEILYGGIPERHIVLVSGGPGTGKSILGKQFLYNGLTKGEGGVFIALEEHPVSVRRSFEHFKWDVRKYEREGKFAIIDTFTGGIGNVAQREKYVVKSIDDVKELSENIRAAIKDINATRIVVDSVSTLYLTKPAMARSIVMQLKRVISGLGCTAIFVSQVSVGERGFGGPGVEHAVDGIIRLDLDEVEGVMYRSIIIWKMRDTKISMVRHPMDITDNGIIVQWDKYLKISNWSVSIQPLPENEISQMKKAVEEAEKEVEVKVEGKEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XC9</accession>
    <name>Q97XC9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1815</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90344">
        <property type="entry name" value="E90344"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XC9"/>
    <dbReference type="STRING" id="273057.SSO1815"/>
    <dbReference type="PaxDb" id="273057-SSO1815"/>
    <dbReference type="EnsemblBacteria" id="AAK42012">
        <property type="protein sequence ID" value="AAK42012"/>
        <property type="gene ID" value="SSO1815"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1815"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1874"/>
    <dbReference type="eggNOG" id="arCOG07181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065738_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XC9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="260" mass="27514" checksum="2141EE6841E51044" modified="2001-10-01" version="1">MPIKIQFFTFRTLSPNATVFFPNAVNQSKVAMGCEVGAGNISAFPKGAAPVFVLVPAFAGLSIFGVPALGATPQGFPTFHYNGLNYTIITQCGAALTETACPDHMSLIYSPAFTVVEQYLGIKNGVFGLPEGVLPTPAHDHLVTFATNTSIPWYIVVVLVFNPNIFPNPITGQCQQVVPSNQSNPTGLCLNNINALEQAMGTYDSAVAAANANNPIWEALGKPGLDVVVPGVTSPSQLFTATNSNMVLFFSDPAVYPYPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XD7</accession>
    <name>Q97XD7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ubiquinone/menaquinone biosynthesis methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1808</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90343">
        <property type="entry name" value="E90343"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991471.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XD7"/>
    <dbReference type="FunCoup" id="Q97XD7">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1808"/>
    <dbReference type="PaxDb" id="273057-SSO1808"/>
    <dbReference type="EnsemblBacteria" id="AAK42004">
        <property type="protein sequence ID" value="AAK42004"/>
        <property type="gene ID" value="SSO1808"/>
    </dbReference>
    <dbReference type="GeneID" id="1454783"/>
    <dbReference type="KEGG" id="sso:SSO1808"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1866"/>
    <dbReference type="eggNOG" id="arCOG04348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_12_0_2"/>
    <dbReference type="InParanoid" id="Q97XD7"/>
    <dbReference type="PhylomeDB" id="Q97XD7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023576">
        <property type="entry name" value="UbiE/COQ5_MeTrFase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004452">
        <property type="entry name" value="PRK05785.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01183">
        <property type="entry name" value="UBIE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0830">Ubiquinone</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="232" mass="27370" checksum="B82651A8C734DA9F" modified="2001-10-01" version="1">MTNKAIKATNEELQQVYNDIPKAYDRANRFISFNQDVKWRADLVKTILKYCKRPKLILDVASGKGELSYTFKKIYKDNSNYEIILSDYAENMLKMALIKDDKVLCSFDALPFREKTFDIVMSSFALHASDNIEDVIREITRVSRKIVGFIAMGKPDSWVRRIYLSIYLKYIMPYIAVLGGAKARDFKYIYYIYERVPTNSQHKRIFEKYIDIKVYEERALNLFYFVVGFPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XE5</accession>
    <name>Q97XE5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA-binding protein, conserved dbpA homolog (DbpA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dbpA</name>
        <name evidence="1" type="ordered locus">SSO8948</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90342">
        <property type="entry name" value="E90342"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989358.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XE5"/>
    <dbReference type="SMR" id="Q97XE5"/>
    <dbReference type="PaxDb" id="273057-SSO8948"/>
    <dbReference type="EnsemblBacteria" id="AAK41996">
        <property type="protein sequence ID" value="AAK41996"/>
        <property type="gene ID" value="SSO8948"/>
    </dbReference>
    <dbReference type="GeneID" id="1454775"/>
    <dbReference type="KEGG" id="sso:SSO8948"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1857"/>
    <dbReference type="eggNOG" id="arCOG07309">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_195721_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XE5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008848">
        <property type="entry name" value="Plasmid_regulator_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05584">
        <property type="entry name" value="Sulfolobus_pRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="73" mass="8872" checksum="FBFA84B3D2579BA8" modified="2001-10-01" version="1">MQKLQNTEKILIILSHGCATLERLEEKTGIPREELLVYLTRLYNRGLIYRKWQKYGGKKFREYCLKNRDEILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XE6</accession>
    <name>Q97XE6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1234</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1801</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90342">
        <property type="entry name" value="D90342"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923598.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XE6"/>
    <dbReference type="PaxDb" id="273057-SSO1801"/>
    <dbReference type="EnsemblBacteria" id="AAK41995">
        <property type="protein sequence ID" value="AAK41995"/>
        <property type="gene ID" value="SSO1801"/>
    </dbReference>
    <dbReference type="GeneID" id="1454774"/>
    <dbReference type="KEGG" id="sso:SSO1801"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1856"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075479_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XE6"/>
    <dbReference type="PhylomeDB" id="Q97XE6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="319" mass="36818" checksum="6FCE7094E2D1A0C2" modified="2001-10-01" version="1">MGVEGSTMARTLRHIPNLMYYPLPPIEDMPWREKWLTEIKPVLDAMDLERVLGEGALVYLKLLIVMVLYSCSYRDAVKMVNVNVVVAWFVGRKVGKSTLHDFVGRLYGVRKKLLEISFKLEEKCLPSYLPASAHLVDFMAWLVDSFLLDLPPGKRSVETFREKAELERREGNLERARKLLSLGRTKRRFEGRWTKKRGVSHYGLKAVAVISVSLFVRSITVKPANFSDKRFKSPLKGIKITDRGFSPSPTQLIAREKPFTTLRVHVEFFGTYLNAFWRPYGTTTWRNDVFLHVLGVIYNIKMFLAIQRRTPPGRRAVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XH3</accession>
    <name>Q97XH3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8875</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90338">
        <property type="entry name" value="B90338"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XH3"/>
    <dbReference type="PaxDb" id="273057-SSO8875"/>
    <dbReference type="DNASU" id="1454740"/>
    <dbReference type="EnsemblBacteria" id="AAK41961">
        <property type="protein sequence ID" value="AAK41961"/>
        <property type="gene ID" value="SSO8875"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8875"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1816"/>
    <dbReference type="eggNOG" id="arCOG08588">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2534836_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XH3"/>
    <dbReference type="PhylomeDB" id="Q97XH3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="83" mass="9544" checksum="00023D63C9C9FF12" modified="2001-10-01" version="1">MEGEIRDSKMFMDFLGGKIPPKSTVFSFYKKLQQTVIQEGETMRTTLMDELNKALDKAISEYREKGFELEVGREKTIGSRTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XL4</accession>
    <name>Q97XL4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1712</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90332">
        <property type="entry name" value="F90332"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923675.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XL4"/>
    <dbReference type="PaxDb" id="273057-SSO1712"/>
    <dbReference type="EnsemblBacteria" id="AAK41917">
        <property type="protein sequence ID" value="AAK41917"/>
        <property type="gene ID" value="SSO1712"/>
    </dbReference>
    <dbReference type="GeneID" id="1454696"/>
    <dbReference type="KEGG" id="sso:SSO1712"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1761"/>
    <dbReference type="eggNOG" id="arCOG07735">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_709075_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XL4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="446" mass="52054" checksum="49DDB1DA0C464E8E" modified="2001-10-01" version="1">MVDDTHKIFQNCDEKNDIYYPICNKLQILCPRLVPGPLWRLSIANISRMSPQAALAICDSCSEIIEKISQYWMSLDRSGKCEVCNKLGREIDEVWLYCVIDENGNIVSNITTKENFTLTESRLYKGIAYLQRLELLCEKCHIAKHQGYALVHGRKQEALEQLAQIHKLDLNKTEELVKEAFFIHGKISKIKEWTIKIGELNGLDKELRLRVEELLNIMYRKGFFVDGRWLYYQYPNYYQEVEPRIIQETMTVLAKTSNKAGTTNVADKWIESLLEIIREELEPKGIRVLPHEFKLFIKYLLEDKKLSNLLQGMFNYALQGKSELFATYISLLDYDDLIGKWMVFVPTDLYPKIFRYMLEALEKAKLAYSAKIVSSRDQYTSKGELPIIIYVPVSFATSYIAEVAKVMKNTLENHHISKNMFFKPDLFTEKGIYSSNANYRSYIYIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XP5</accession>
    <name>Q97XP5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90327">
        <property type="entry name" value="G90327"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923556.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XP5"/>
    <dbReference type="STRING" id="273057.SSO1666"/>
    <dbReference type="PaxDb" id="273057-SSO1666"/>
    <dbReference type="EnsemblBacteria" id="AAK41878">
        <property type="protein sequence ID" value="AAK41878"/>
        <property type="gene ID" value="SSO1666"/>
    </dbReference>
    <dbReference type="GeneID" id="1454657"/>
    <dbReference type="KEGG" id="sso:SSO1666"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1715"/>
    <dbReference type="eggNOG" id="arCOG07255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1943947_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XP5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="128" mass="14561" checksum="9D61797AA75E4791" modified="2001-10-01" version="1">MKLSDNYRIFTISNIIVGLIFSILYFITSGFIQYYNLVYGILTLGIAIWGIGRYYFKKIEDDKIRAGVQTAWLIVSFALGYISIIYAPVLFTKLEIIVIESILSIIQVLWGSALLAISYRKGYSVIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XW0</accession>
    <name>Q97XW0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">First ORF in transposon ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1601</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90319">
        <property type="entry name" value="B90319"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923498.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XW0"/>
    <dbReference type="FunCoup" id="Q97XW0">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1601"/>
    <dbReference type="PaxDb" id="273057-SSO1601"/>
    <dbReference type="EnsemblBacteria" id="AAK41809">
        <property type="protein sequence ID" value="AAK41809"/>
        <property type="gene ID" value="SSO1601"/>
    </dbReference>
    <dbReference type="GeneID" id="1454588"/>
    <dbReference type="KEGG" id="sso:SSO1601"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1637"/>
    <dbReference type="eggNOG" id="arCOG04431">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020019_3_2_2"/>
    <dbReference type="InParanoid" id="Q97XW0"/>
    <dbReference type="PhylomeDB" id="Q97XW0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015254">
        <property type="term" value="F:glycerol channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015793">
        <property type="term" value="P:glycerol transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050363">
        <property type="entry name" value="MIP/Aquaporin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829:SF9">
        <property type="entry name" value="AQUAPORIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="289" mass="32216" checksum="5CAC017B5463FCA8" modified="2001-10-01" version="1">MSVVLKDSLWRYFAEFVGTFILILFGDGAIVASTLSSQPSFGFIMVSWGFGIVLAIYAVGPISGAHINPNVTLAFAVTKRIKWVDVIPYIVFQILGAAAATSVLLVWWGDVITRIDPPPFLYADIGAAFFTQYPEPGFWPQYWPKSYLPNVSSAGILYSQVNQIFPLWKGAFAEALMTFLLLLIVVAVTDPDSPFYSQSLAPWAIGIGYVMPFLLFEAQLTGGMINEARSWGPMLALYLFGYRTGAFNFRGEYFYVYGIPDFIGGILGALFWDHVLKPYLRFVKNINKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-26" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XW1</accession>
    <name>GLPK1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycerol kinase 1</fullName>
            <ecNumber evidence="1">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP:glycerol 3-phosphotransferase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycerokinase 1</fullName>
            <shortName evidence="1">GK 1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glpK1</name>
        <name type="ordered locus">SSO1600</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycerol + ATP = sn-glycerol 3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99319">
        <property type="entry name" value="A99319"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923497.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XW1"/>
    <dbReference type="SMR" id="Q97XW1"/>
    <dbReference type="FunCoup" id="Q97XW1">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1600"/>
    <dbReference type="PaxDb" id="273057-SSO1600"/>
    <dbReference type="EnsemblBacteria" id="AAK41808">
        <property type="protein sequence ID" value="AAK41808"/>
        <property type="gene ID" value="SSO1600"/>
    </dbReference>
    <dbReference type="GeneID" id="1454587"/>
    <dbReference type="KEGG" id="sso:SSO1600"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1636"/>
    <dbReference type="eggNOG" id="arCOG00024">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_2_3_2"/>
    <dbReference type="InParanoid" id="Q97XW1"/>
    <dbReference type="PhylomeDB" id="Q97XW1"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07786">
        <property type="entry name" value="FGGY_EcGK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000007">
        <property type="entry name" value="Glycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000008">
        <property type="entry name" value="Glycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00186">
        <property type="entry name" value="Glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATPase_NBD"/>
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        <property type="entry name" value="Carb_kinase_FGGY"/>
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        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
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        <property type="entry name" value="FGGY_N"/>
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        <property type="entry name" value="Glycerol_kin"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRK00047.1"/>
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    </dbReference>
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        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Actin-like ATPase domain"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0319">Glycerol metabolism</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000059533" description="Glycerol kinase 1">
        <location>
            <begin position="1"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
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        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>sn-glycerol 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
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        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
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            <name>ADP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>glycerol</name>
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        </ligand>
    </feature>
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        <location>
            <position position="84"/>
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        <ligand>
            <name>sn-glycerol 3-phosphate</name>
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        </ligand>
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        <ligand>
            <name>glycerol</name>
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        </ligand>
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        </ligand>
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    <feature type="binding site" evidence="1">
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            <position position="135"/>
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        <ligand>
            <name>glycerol</name>
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        </ligand>
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        <location>
            <position position="242"/>
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            <name>ATP</name>
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            <name>ATP</name>
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        <location>
            <position position="408"/>
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        <ligand>
            <name>ADP</name>
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        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00186"/>
        </source>
    </evidence>
    <sequence length="501" mass="55681" checksum="3A27B3EF44371E88" modified="2001-10-01" version="1">MNTMSHKFVLALDEGTTSARAILFDSDLNIVNIGQYEFPQYYPQPGYVEHDPEEIWEAQMLAVKKAISKIDAKQIVAIGITNQRETTVLWDAKSGKPVYNAIVWQDRRTSPITDWLKANYFKMIKDKTGLVPDPYFSASKIKWILDNVPNVREKAERGEIKFGTLDTYLIWRLTNGKAHVTDYSNASRTMLFNINKLECDREILELLKIPESILPEVKPSSEIYGYSEALGNLIPISGDAGDQQAALFGQVAFNVGEIKATYGTGSFILMNIGNNPIRSENLLTTIAWGLEKNKATYALEGSIFITGAAVQWFRDGLRAIDVSDEIEPLASNVEDNGGVYFVPAFVGLGAPYWDPYARGLIIGITRGTTKAHIARAILESMAYQTRDVIEVMQKESGISINSLKVDGGAAKDNLLMQFQADILGIKVIRPKVMETTSMGVAMLAGLGVGLWNSLEELRSIWKVDKEFIPSMSEEKRRALYSGWKEAVKRAMGWAKVVGGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y22</accession>
    <name>Q97Y22_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="4">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pdhB-2</name>
        <name evidence="9" type="ordered locus">SSO1526</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
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            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="F90311"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    </dbReference>
    <dbReference type="STRING" id="273057.SSO1526"/>
    <dbReference type="PaxDb" id="273057-SSO1526"/>
    <dbReference type="EnsemblBacteria" id="AAK41749">
        <property type="protein sequence ID" value="AAK41749"/>
        <property type="gene ID" value="SSO1526"/>
    </dbReference>
    <dbReference type="GeneID" id="1454528"/>
    <dbReference type="KEGG" id="sso:SSO1526"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1566"/>
    <dbReference type="eggNOG" id="arCOG01052">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_012907_1_0_2"/>
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    <dbReference type="PhylomeDB" id="Q97Y22"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
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        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
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        <property type="entry name" value="THDP-binding"/>
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        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
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        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
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        <property type="entry name" value="PRK09212.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43257">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="8">
        <location>
            <begin position="4"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="324" mass="35324" checksum="A29A55F00D4428A0" modified="2001-10-01" version="1">MRQITFTEAINEALRQEMERDPSVILIGEDIGVYGGAFGVTKGLIEKFGSDRVIDTPISEAGFIGAAVGAALAGLRPVVELMFVDFFGVAMDQIYNQMAKLRYMSGGQLKVPLTLRAPIGAGISAAAQHSQTLYSIFAHVPGLKVVVPSTPHDAKGLLISSIRDDNPVVFLEHKVLYGIKGEVPEEEYTIPLGKAEIRREGDDVTVIGIARTVWHSLEAAEQLSKESISVEVIDVRSIVPFDKETVIKSVKKTGRVVIVDEDYDRCGFASWVSSIIADEAFEYLDAPIKRITTPNVPIPFSPPLEQYILPDSKKIVNTVKSILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y43</accession>
    <name>Q97Y43_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1505</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="A99309"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923438.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y43"/>
    <dbReference type="FunCoup" id="Q97Y43">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1505"/>
    <dbReference type="PaxDb" id="273057-SSO1505"/>
    <dbReference type="EnsemblBacteria" id="AAK41728">
        <property type="protein sequence ID" value="AAK41728"/>
        <property type="gene ID" value="SSO1505"/>
    </dbReference>
    <dbReference type="GeneID" id="1454507"/>
    <dbReference type="KEGG" id="sso:SSO1505"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1546"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y43"/>
    <dbReference type="PhylomeDB" id="Q97Y43"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17319">
        <property type="entry name" value="MFS_ExuT_GudP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000126">
        <property type="entry name" value="MFS transporter permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF36">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G08340)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="332"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="16"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="421" mass="46426" checksum="43B56517538A7564" modified="2001-10-01" version="1">MKSLVESSRHKRYLRILPILFFLYIVNFLDRVNISYAIDAHMFQYLGVPTRQVGIIASLASSLFFIGYFIPQIFSNLGINRYGVRKIFLAAFTVWGIITILTGFVSSVSQVYVLRFLLGIAEGPFFAGVMFYLSLWFLKPERATANSFFTAAIPVSGIFGGLIAGAIFATYGDYPGWRYLFIYEGILAIIGGILAYFILTDFPKDAKWLSNEEKIAIQNAFKEEETEKVNVKWTKALTNRDVIILAIVYFLGVTSLYGYSIWLPSIISSFGKVNATVASFLSVIPYVIASISLIFIAHLADRTQRHRSITSIVFIIAGVGLGLSALTQNIFVLSFIFFAIAAIGIYSFIATFWAVPQKFLHGDAAAAAIGLINAVGNLGGIVGPIVVGFLKSFTGSFIDGVYMMALFSFLAGLVILLIRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y94</accession>
    <name>Q97Y94_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HD Cas3-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1439</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="A90302"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    <dbReference type="STRING" id="273057.SSO1439"/>
    <dbReference type="PaxDb" id="273057-SSO1439"/>
    <dbReference type="EnsemblBacteria" id="AAK41672">
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        <property type="gene ID" value="SSO1439"/>
    </dbReference>
    <dbReference type="GeneID" id="1454451"/>
    <dbReference type="KEGG" id="sso:SSO1439"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1468"/>
    <dbReference type="eggNOG" id="arCOG01442">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1163752_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y94"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR006483">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HD_CAS3"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD Cas3-type" evidence="4">
        <location>
            <begin position="5"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="244" mass="28009" checksum="1A9543E8A1B5622C" modified="2001-10-01" version="1">MIKPCAYEKQGLIDHAIGSYKVLHDKISESYYKVISRRLERYGIVLDLNGVKEIVKDVVVLHDMGKAGEYYQNQFDDNCNPLKSNPLKSRFSFIYHELGSALFFYNDYEPINVEKAEEVRSLLTLAVINHLNAIRGISDYLLNRFPDNFDEEMIKLSKYGSILLENLRGIISKSLKVRDYSFTDYHDMLYAFSKKSDKYLKLYNLFLAPIMLGDNLDSSLVRNNGSKTRFVGILEGELNGGSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YE1</accession>
    <name>Q97YE1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Second ORF in transposon ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1386</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90295">
        <property type="entry name" value="E90295"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YE1"/>
    <dbReference type="STRING" id="273057.SSO1386"/>
    <dbReference type="PaxDb" id="273057-SSO1386"/>
    <dbReference type="EnsemblBacteria" id="AAK41620">
        <property type="protein sequence ID" value="AAK41620"/>
        <property type="gene ID" value="SSO1386"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1386"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1392"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1302673_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YE1"/>
    <dbReference type="PhylomeDB" id="Q97YE1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="2">
        <location>
            <begin position="126"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="248" mass="28431" checksum="5A7297FDBEC250F3" modified="2001-10-01" version="1">MRIINPLVLKKFKDIRGKKSDKNDKKLAELVISMGSEFTTSEAKELTSQWDFITRSIVRVKNRLRRDLILLGYKDSLSKKNLNEVLRGEDNIVLSEVRFLLDELERLEVRKREIEEELEDLVPKDSLIFTIPGIGRTLGCIILARVGDVKRFGDKKRFVAYCGLDPLVEFSGKSVVSRGISRRGDAVLRRAFYLAALTAIKVNPIIKRFYVEHKENLKGKKLITACARKLAVITWAVLYYNKPFDASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YI5</accession>
    <name>Q97YI5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Second ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1336</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90289">
        <property type="entry name" value="G90289"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923331.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YI5"/>
    <dbReference type="STRING" id="273057.SSO1336"/>
    <dbReference type="PaxDb" id="273057-SSO1336"/>
    <dbReference type="EnsemblBacteria" id="AAK41574">
        <property type="protein sequence ID" value="AAK41574"/>
        <property type="gene ID" value="SSO1336"/>
    </dbReference>
    <dbReference type="GeneID" id="1454353"/>
    <dbReference type="KEGG" id="sso:SSO1336"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1342"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="InParanoid" id="Q97YI5"/>
    <dbReference type="PhylomeDB" id="Q97YI5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF22">
        <property type="entry name" value="TNPB-LIKE PROTEIN MJ1635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="187"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="308"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="423" mass="50129" checksum="209EE7917018EA1D" modified="2001-10-01" version="1">MLKNLRLKSFQPEEEYVHFTYSIKNDKREESKELIKEYTTLLQKAIDYLWSLTKIQVRKNGNYKITLPKKKEVYKPLRDELEKINHLASHYVDKAINDAFSILKSWRKRAIKGRASIEKPRVKKAYVRIKTTLRKVVGESVRITVRPYEYITFSWSKSWFSRRVRELELGEPIIKEEKVYLPFRYKLPWATPLNFLAIDSNLYTLDAYDGEKFVTISLKQLYSLKYSMEVKRAKVQSFASKHTKRGRELLRKYSHRERNRVLDFVHKFVNALLDLYPMTFFAVEKLNKESMFVDANDSLSRKISRTVWRSIHRVLKYKAPLYGSFVKEVNPRFTSRSCPRCGFVSRKVGKTFECERCGFKLDRQLNASLNIYLKMCGFPHIRDIARVWVGVIPLMGRRGMNGFPRDFGEAQGLRIDIKYHEIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YJ6</accession>
    <name>Q97YJ6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helicase HerA central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1327</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90288">
        <property type="entry name" value="D90288"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923320.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YJ6"/>
    <dbReference type="PaxDb" id="273057-SSO1327"/>
    <dbReference type="EnsemblBacteria" id="AAK41563">
        <property type="protein sequence ID" value="AAK41563"/>
        <property type="gene ID" value="SSO1327"/>
    </dbReference>
    <dbReference type="GeneID" id="1454342"/>
    <dbReference type="KEGG" id="sso:SSO1327"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1327"/>
    <dbReference type="eggNOG" id="arCOG00284">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_453183_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YJ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00267">
        <property type="entry name" value="ABC_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051162">
        <property type="entry name" value="T4SS_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121:SF1">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJGR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase HerA central" evidence="1">
        <location>
            <begin position="155"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="596" mass="67754" checksum="93EEE3847BB9EB54" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YM6</accession>
    <name>Q97YM6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1297</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90284">
        <property type="entry name" value="G90284"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923294.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YM6"/>
    <dbReference type="STRING" id="273057.SSO1297"/>
    <dbReference type="TCDB" id="9.B.323.2.1">
        <property type="family name" value="the putative abc3 auxiliary protein (abc3-ap) family"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1297"/>
    <dbReference type="EnsemblBacteria" id="AAK41534">
        <property type="protein sequence ID" value="AAK41534"/>
        <property type="gene ID" value="SSO1297"/>
    </dbReference>
    <dbReference type="GeneID" id="1454313"/>
    <dbReference type="KEGG" id="sso:SSO1297"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1297"/>
    <dbReference type="eggNOG" id="arCOG02079">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_397745_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YM6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="660"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="692" mass="78012" checksum="960B8B7971CBAB1F" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YS3</accession>
    <name>Q97YS3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1243</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90278">
        <property type="entry name" value="F90278"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993017.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YS3"/>
    <dbReference type="PaxDb" id="273057-SSO1243"/>
    <dbReference type="EnsemblBacteria" id="AAK41485">
        <property type="protein sequence ID" value="AAK41485"/>
        <property type="gene ID" value="SSO1243"/>
    </dbReference>
    <dbReference type="GeneID" id="1454264"/>
    <dbReference type="KEGG" id="sso:SSO1243"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1240"/>
    <dbReference type="eggNOG" id="arCOG00729">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121774_5_0_2"/>
    <dbReference type="InParanoid" id="Q97YS3"/>
    <dbReference type="PhylomeDB" id="Q97YS3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09873">
        <property type="entry name" value="PIN_Pae0151-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044153">
        <property type="entry name" value="PIN_Pae0151-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051619">
        <property type="entry name" value="TypeII_TA_RNase_PINc/VapC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901:SF1">
        <property type="entry name" value="EXONUCLEASE VAPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901">
        <property type="entry name" value="RIBONUCLEASE VAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="2">
        <location>
            <begin position="23"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="130" mass="15118" checksum="15F6B4A4970B9115" modified="2001-10-01" version="1">MKDKEFLLDASALYSLLDYVDKVDVKKIHVLTLTFYEVGNVIWKEYYIHKKVKDPITLSRLFYKLMRKFNVIEDSPLEGVMRIAIERGLTYYNASYAYVAESLGLILVSNDKELIRKANAISLKDLIKSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YT6</accession>
    <name>Q97YT6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acetoacetate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1221</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90276">
        <property type="entry name" value="E90276"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923259.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YT6"/>
    <dbReference type="STRING" id="273057.SSO1221"/>
    <dbReference type="PaxDb" id="273057-SSO1221"/>
    <dbReference type="EnsemblBacteria" id="AAK41468">
        <property type="protein sequence ID" value="AAK41468"/>
        <property type="gene ID" value="SSO1221"/>
    </dbReference>
    <dbReference type="GeneID" id="1454247"/>
    <dbReference type="KEGG" id="sso:SSO1221"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1222"/>
    <dbReference type="eggNOG" id="arCOG04489">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091354_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YT6"/>
    <dbReference type="PhylomeDB" id="Q97YT6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.400.10">
        <property type="entry name" value="Acetoacetate decarboxylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010451">
        <property type="entry name" value="Acetoacetate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023375">
        <property type="entry name" value="ADC_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06314">
        <property type="entry name" value="ADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160104">
        <property type="entry name" value="Acetoacetate decarboxylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="30750" checksum="AEE0695D2EE5A505" modified="2001-10-01" version="1">MEEIRPYYNTFSLPITKSGKSQIVPPPPWIYAIEMIGVKAYFDPVKVLDLVPPPLEIVDGEGFVYIAKIFTVSGNRWEMLYEDPEETKYMEAAIALKVKYNDNIFTYFPFMWVDKDLPLLRGWLLGYPKKLAYISISEFHKLLDGYSGPSSGVRMGGYALRNGKEIIRVKITLKEKVSQYPIPFGPIVQFRRFPAVQDGTDVYELGQIISSDFRVGEVWRGEGEVILNGSVNEELEVLKINRIEGGYYFNWSFKQLGTKILTKVPNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z16</accession>
    <name>Q97Z16_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Heterodisulfide reductase, subunit C (HdrC-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hdrC-1</name>
        <name evidence="8" type="ordered locus">SSO1127</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HdrC family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90265">
        <property type="entry name" value="G90265"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989757.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z16"/>
    <dbReference type="FunCoup" id="Q97Z16">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1127"/>
    <dbReference type="PaxDb" id="273057-SSO1127"/>
    <dbReference type="EnsemblBacteria" id="AAK41382">
        <property type="protein sequence ID" value="AAK41382"/>
        <property type="gene ID" value="SSO1127"/>
    </dbReference>
    <dbReference type="GeneID" id="1454161"/>
    <dbReference type="KEGG" id="sso:SSO1127"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1122"/>
    <dbReference type="eggNOG" id="arCOG00965">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086560_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z16"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051460">
        <property type="entry name" value="HdrC_iron-sulfur_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255:SF1">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="55"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="280" mass="32510" checksum="455CE716458AE7C1" modified="2001-10-01" version="1">MATKTILPPLPDDPRFLDMSYDVQAPLPEEERNLLSNLKPEEREVAVKFWEAVKSDFRYNEYLRGCLNCGVCTSGCPAAKFYDFGPREMIQYMMRDEVDKIWEFVNKKVWACVQCYTCSMRCPFNNEIAGLIMVLREYAVKFGLQSAKEVLAPYRRVLLTVITTGNQVTPDMIQPDAFPDWGPQAVEESKNMDVYRKAVPVDLLQRTDIGWHASLQTAVEMMTIFVEAGVLDSLKNVDKDLYDMIMDIYEERKQQLEEIKEKWEKGELKEEDLPDSWLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z29</accession>
    <name>Q97Z29_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CoA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99264">
        <property type="entry name" value="A99264"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989744.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z29"/>
    <dbReference type="FunCoup" id="Q97Z29">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1111"/>
    <dbReference type="PaxDb" id="273057-SSO1111"/>
    <dbReference type="EnsemblBacteria" id="AAK41368">
        <property type="protein sequence ID" value="AAK41368"/>
        <property type="gene ID" value="SSO1111"/>
    </dbReference>
    <dbReference type="GeneID" id="1454147"/>
    <dbReference type="KEGG" id="sso:SSO1111"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1108"/>
    <dbReference type="eggNOG" id="arCOG04227">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112567_0_1_2"/>
    <dbReference type="InParanoid" id="Q97Z29"/>
    <dbReference type="PhylomeDB" id="Q97Z29"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303:SF2">
        <property type="entry name" value="COA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303">
        <property type="entry name" value="CYTOPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="1">
        <location>
            <begin position="12"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="154" mass="17447" checksum="964C7EDCF7621035" modified="2001-10-01" version="1">MENEEEVIKEVLLKYKNIATVGFSKDPSKAAFQVPKFLMDHGYNVIPVNPSASEILGKKSYPSILDVPDKVEIVEIFRPSNEVPKIVDQVLERVKKVGDVKVIWMQEGIRNDEAAEKARKAGLVVIQDRCMYKEYMKKIFNVNNPPPVSSLKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z41</accession>
    <name>Q97Z41_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90262">
        <property type="entry name" value="D90262"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989729.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z41"/>
    <dbReference type="STRING" id="273057.SSO1096"/>
    <dbReference type="PaxDb" id="273057-SSO1096"/>
    <dbReference type="EnsemblBacteria" id="AAK41355">
        <property type="protein sequence ID" value="AAK41355"/>
        <property type="gene ID" value="SSO1096"/>
    </dbReference>
    <dbReference type="GeneID" id="1454134"/>
    <dbReference type="KEGG" id="sso:SSO1096"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1093"/>
    <dbReference type="eggNOG" id="arCOG01212">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1324042_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z41"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="27847" checksum="E225D5C36FD64B09" modified="2001-10-01" version="1">MSEILEICKKVKLLAYFGSYTRQEDFVEGISDINVFLISEDKYLILELASLGYSPIELSEKSFIDLCEKGDPICYYLLYDSNVVCGQFPKSVKFNLNDFTCERIRRSIFPLLSLSVSSFLRQDEISAVSNSFRALRSIIQWRSCTDLKTIPLRNDDLRDRCRELNLSICNEFSDIVLIRRRKAPLSLWSLDKIVEAICSELKISCTKPSKILQVVKNPIQVTYKEDGRVTVKDSLNRETKIA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z90</accession>
    <name>Y1041_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0200 protein SSO1041</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO1041</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0200 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90255">
        <property type="entry name" value="H90255"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989191.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3H0K">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.25 A"/>
        <property type="chains" value="A/B=9-186"/>
    </dbReference>
    <dbReference type="PDB" id="3LW7">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B=8-186"/>
    </dbReference>
    <dbReference type="PDBsum" id="3H0K"/>
    <dbReference type="PDBsum" id="3LW7"/>
    <dbReference type="AlphaFoldDB" id="Q97Z90"/>
    <dbReference type="SMR" id="Q97Z90"/>
    <dbReference type="FunCoup" id="Q97Z90">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1041"/>
    <dbReference type="PaxDb" id="273057-SSO1041"/>
    <dbReference type="EnsemblBacteria" id="AAK41303">
        <property type="protein sequence ID" value="AAK41303"/>
        <property type="gene ID" value="SSO1041"/>
    </dbReference>
    <dbReference type="GeneID" id="84058567"/>
    <dbReference type="KEGG" id="sso:SSO1041"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1035"/>
    <dbReference type="eggNOG" id="arCOG01045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096329_1_0_2"/>
    <dbReference type="InParanoid" id="Q97Z90"/>
    <dbReference type="PhylomeDB" id="Q97Z90"/>
    <dbReference type="EvolutionaryTrace" id="Q97Z90"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02022">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01111">
        <property type="entry name" value="UPF0200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022970">
        <property type="entry name" value="NTP_hydrolase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930:SF1">
        <property type="entry name" value="DEPHOSPHO-COA KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930">
        <property type="entry name" value="UPF0200 PROTEIN MJ1399"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000094534" description="UPF0200 protein SSO1041">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="15"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="10"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="34"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="88"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="110"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="119"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="153"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="161"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="170"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01111"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="3LW7"/>
        </source>
    </evidence>
    <sequence length="188" mass="21485" checksum="F25D094528A821E5" modified="2001-10-01" version="1">MSYLVVTIKVILITGMPGSGKSEFAKLLKERGAKVIVMSDVVRKRYSIEAKPGERLMDFAKRLREIYGDGVVARLCVEELGTSNHDLVVFDGVRSLAEVEEFKRLLGDSVYIVAVHSPPKIRYKRMIERLRSDDSKEISELIRRDREELKLGIGEVIAMADYIITNDSNYEEFKRRCEEVTDRVLKNG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZH1</accession>
    <name>Y942_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative HTH-type transcriptional regulatory protein SSO0942</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0942</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90245">
        <property type="entry name" value="C90245"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992376.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZH1"/>
    <dbReference type="SMR" id="Q97ZH1"/>
    <dbReference type="FunCoup" id="Q97ZH1">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0942"/>
    <dbReference type="PaxDb" id="273057-SSO0942"/>
    <dbReference type="EnsemblBacteria" id="AAK41218">
        <property type="protein sequence ID" value="AAK41218"/>
        <property type="gene ID" value="SSO0942"/>
    </dbReference>
    <dbReference type="GeneID" id="1455185"/>
    <dbReference type="KEGG" id="sso:SSO0942"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.938"/>
    <dbReference type="eggNOG" id="arCOG04152">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075726_1_0_2"/>
    <dbReference type="InParanoid" id="Q97ZH1"/>
    <dbReference type="PhylomeDB" id="Q97ZH1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00584">
        <property type="entry name" value="HTH_type_cro_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020886">
        <property type="entry name" value="MTH_967-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059051">
        <property type="entry name" value="MTH_967_PDDEXK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26553">
        <property type="entry name" value="PDDEXK_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000144866" description="Putative HTH-type transcriptional regulatory protein SSO0942">
        <location>
            <begin position="1"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="125"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00584"/>
        </source>
    </evidence>
    <sequence length="310" mass="35386" checksum="F8DD43C3C069DA39" modified="2001-10-01" version="1">MSKKIIDEVIDILEDKKYTYTMIEYPEHNRKSVDIVLNSKEPTLIRVSEDKITKEEISDLKKIAVSTLTASLVVTNEEEEDIVSVKADNVFAISPEGFKKVINGEKIFLYRTRGGIFIKIRNYILKHKREEMGYSIGDVAKFLGVSRKAIYDYEKGDSDVSLEVAEKLIDLFGDDIIGDVIWDSIKSKKEILEEGVAEFSPENFKAKLIYKLKESGLNALSLKLTAADLIVKDSDNNRYLVTIENKDYNKSMKKFYEAKKLASYTKSELVIIIRTSKMLKECEDLGYKTYEENDIHSLIDEIKGSNGRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZT3</accession>
    <name>Q97ZT3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Pyrroline-5-carboxylate reductase</fullName>
            <shortName evidence="4">P5C reductase</shortName>
            <shortName evidence="4">P5CR</shortName>
            <ecNumber evidence="4 5">1.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">PCA reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 9" type="primary">proC</name>
        <name evidence="9" type="ordered locus">SSO0495</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-proline + NAD(+) = (S)-1-pyrroline-5-carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="4 5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90194">
        <property type="entry name" value="H90194"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZT3"/>
    <dbReference type="FunCoup" id="Q97ZT3">
        <property type="interactions" value="217"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0495"/>
    <dbReference type="PaxDb" id="273057-SSO0495"/>
    <dbReference type="EnsemblBacteria" id="AAK40815">
        <property type="protein sequence ID" value="AAK40815"/>
        <property type="gene ID" value="SSO0495"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0495"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.488"/>
    <dbReference type="eggNOG" id="arCOG00455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042344_3_1_2"/>
    <dbReference type="InParanoid" id="Q97ZT3"/>
    <dbReference type="PhylomeDB" id="Q97ZT3"/>
    <dbReference type="BRENDA" id="1.5.1.2">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.10:FF:000001">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000866">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF0">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 6" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="7">
        <location>
            <begin position="8"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="8">
        <location>
            <begin position="159"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK40815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="277" mass="30519" checksum="3A42E2BEE2AAD4D1" modified="2001-10-01" version="1">MKIMEDLTIGILGAGKIGSAIVRAIRLKYPSIHVIATARKDETLRNVKDLEVETTKDNNYAVNKSDVIILSVKPQHFPTVLRQVGIESWRDKVVISIMAGVKLSTLSTLLNNAEVYRAMPNINAIVYKSTTTIAENNGKSRELVENIFKTLGNVYWLPEEYLDIWTALVGSGPAFISEIIDALSLGAVACGMQREIAYNAVLDMISGTIIMLKEGKIDHPMLLRDQVTTPVGTTIRGLMVMEAKSVKSALIETIEASYKRAVEIGNEIDKSIRNNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZV8</accession>
    <name>Q97ZV8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiamine-phosphate synthase ThiN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0468</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90191">
        <property type="entry name" value="G90191"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZV8"/>
    <dbReference type="STRING" id="273057.SSO0468"/>
    <dbReference type="PaxDb" id="273057-SSO0468"/>
    <dbReference type="EnsemblBacteria" id="AAK40790">
        <property type="protein sequence ID" value="AAK40790"/>
        <property type="gene ID" value="SSO0468"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0468"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.462"/>
    <dbReference type="eggNOG" id="arCOG00021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054903_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZV8"/>
    <dbReference type="PhylomeDB" id="Q97ZV8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019293">
        <property type="entry name" value="ThiN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40730:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40730">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10120">
        <property type="entry name" value="ThiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine-phosphate synthase ThiN" evidence="1">
        <location>
            <begin position="145"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="314" mass="35650" checksum="C53D52BF01C3F9FE" modified="2001-10-01" version="1">MFDLNLTIVLKSPLSIFDDIFITSIRVLEAKRLRELHMSQTRIANLLGVSQPAVKQYLDEDENSYIKRLQNLGLTREEIDDFLDKLTQLLINGDPKQVMQYISVFFLSNLSRLKFCKYHKMIDSEIPVDCDICKSLYKENEEEMMELALSMLQNESVAELIPEVLSNLAFAKANAKREEDVLAIPGRITKIRNLPTPASKPMWGGSKHLAKVLLSVMKNHPAVRSVMNIKYDEKVEMILKEIGYKFKKVGPQNDLGNERIAETIAAVFDEGTDVVIHLGGTGIEANTYVFGRDPLDVVKKIINISVKYKEIFSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-10-02" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q980A4</accession>
    <name>Q980A4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90185">
        <property type="entry name" value="F90185"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980A4"/>
    <dbReference type="STRING" id="273057.SSO0414"/>
    <dbReference type="PaxDb" id="273057-SSO0414"/>
    <dbReference type="EnsemblBacteria" id="AAK40741">
        <property type="protein sequence ID" value="AAK40741"/>
        <property type="gene ID" value="SSO0414"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0414"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.408"/>
    <dbReference type="eggNOG" id="arCOG04312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107892_0_0_2"/>
    <dbReference type="InParanoid" id="Q980A4"/>
    <dbReference type="PhylomeDB" id="Q980A4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09879">
        <property type="entry name" value="PIN_VapC_AF0591-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041120">
        <property type="entry name" value="PIN_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18477">
        <property type="entry name" value="PIN_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="12"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="145" mass="17141" checksum="104078632EEF914C" modified="2001-10-01" version="1">MENDRMGFSRDLKVLVDTNILLYVYDGLDPFNKVLEFLDYKPSFFIHSTVLRELDILFEKNKKGFIISSRIKIARKYLEVYKNLWNLINDYDDLPTDEALIRTALKHNMFIFTNDKELKNDAIKKGIGVLFLQNRSKIIKSLYPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q980B7</accession>
    <name>Q980B7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-ethylammeline chlorohydrolase related protein (TrzA-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">trzA-like</name>
        <name evidence="2" type="ordered locus">SSO0398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90183">
        <property type="entry name" value="H90183"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988792.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980B7"/>
    <dbReference type="STRING" id="273057.SSO0398"/>
    <dbReference type="PaxDb" id="273057-SSO0398"/>
    <dbReference type="DNASU" id="1455537"/>
    <dbReference type="EnsemblBacteria" id="AAK40727">
        <property type="protein sequence ID" value="AAK40727"/>
        <property type="gene ID" value="SSO0398"/>
    </dbReference>
    <dbReference type="GeneID" id="1455537"/>
    <dbReference type="KEGG" id="sso:SSO0398"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.394"/>
    <dbReference type="eggNOG" id="arCOG00692">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_1_0_2"/>
    <dbReference type="InParanoid" id="Q980B7"/>
    <dbReference type="PhylomeDB" id="Q980B7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01305">
        <property type="entry name" value="archeal_chlorohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050287">
        <property type="entry name" value="MTA/SAH_deaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794">
        <property type="entry name" value="AMINOHYDROLASE SSNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794:SF5">
        <property type="entry name" value="CHLOROHYDROLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="53"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="379" mass="42649" checksum="F385B64E2E02C7AB" modified="2001-10-01" version="1">MAYGGRITLNVRIALVGEELEIRENVNIEIEEGIITHIGNGFSSEGITFKNGILIPGIVNAHVHSADFICQEMGYNMPISKVVGDPHSVKYECFAKNTKESIMNSIERFVLRARELGSNVMIDFREQGLEGSLISSTIKKKMATNGVKYYFLGRLEEDEFKDIRNLNSLYKIADGYGLSSAFSTSEIELIKHVFNNKIRSVHVSETIKHWLRDDLEYVMKRYEPNLIIHGTYLSEEEIDIMRYRKASIVYCPRSNLWFSVGIPKVINGLKSGVNLLIGTDNGGVLDPDMWKEMETLLLISRVQDPLSDHSLQILKASTINAYKFLGIRGWIEEGNPIEAGLLVLEGDSTGILNSNNKYIGIIKRGNKIIYNLGAIQKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q980E7</accession>
    <name>Q980E7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MarR family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0365</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90179">
        <property type="entry name" value="H90179"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980E7"/>
    <dbReference type="SMR" id="Q980E7"/>
    <dbReference type="STRING" id="273057.SSO0365"/>
    <dbReference type="PaxDb" id="273057-SSO0365"/>
    <dbReference type="EnsemblBacteria" id="AAK40695">
        <property type="protein sequence ID" value="AAK40695"/>
        <property type="gene ID" value="SSO0365"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0365"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.359"/>
    <dbReference type="eggNOG" id="arCOG01804">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053800_0_0_2"/>
    <dbReference type="InParanoid" id="Q980E7"/>
    <dbReference type="PhylomeDB" id="Q980E7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014466">
        <property type="entry name" value="Txn_rg_UCP012876"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30024">
        <property type="entry name" value="ALIPHATIC SULFONATES-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30024:SF42">
        <property type="entry name" value="ALIPHATIC SULFONATES-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012876">
        <property type="entry name" value="Txn_rg_UCP012876"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="34420" checksum="31CC215678828417" modified="2001-10-01" version="1">MLMGSKDKVISILKEKGPISQTELSKLSGLSKSRLSEVLSELEKQGIVKRRKVLGKNLEVSLSQDRFLRLGIIRAAEYPFIIPFIKNLENRGYSVTVKIYENGIDLTRDLVEAKLDLGLSPIITQIIFSTIYRNIRIIGGGAKGGGGIIGKTCNRIASTVMSSMEIWAFSEFKNIEIIPSYSSYEMLDFLEKGIVDGIAIWEPFLSILERKGHKVHRFTPLHCCTLAVRETMDWERIKNIYEESFSWFKSSIDRWVSSYSNLLNIDYNILKEASKNYEFDYYLDLKEFKNSLKNTGIFIPI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2026-01-28" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q980F7</accession>
    <name>RS19E_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein eS19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 5" type="primary">rps19e</name>
        <name evidence="5" type="ordered locus">SSO0353</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="8 9 10" key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="function">
        <text evidence="1">May be involved in maturation of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90178">
        <property type="entry name" value="F90178"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="U=1-158"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="U=1-158"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="U=1-158"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="U=1-158"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="U=1-158"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="U=1-158"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="U=1-158"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="U=1-158"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q980F7"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q980F7"/>
    <dbReference type="FunCoup" id="Q980F7">
        <property type="interactions" value="216"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0353"/>
    <dbReference type="PaxDb" id="273057-SSO0353"/>
    <dbReference type="DNASU" id="1455495"/>
    <dbReference type="EnsemblBacteria" id="AAK40685">
        <property type="protein sequence ID" value="AAK40685"/>
        <property type="gene ID" value="SSO0353"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0353"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.346"/>
    <dbReference type="eggNOG" id="arCOG01344">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108559_1_0_2"/>
    <dbReference type="InParanoid" id="Q980F7"/>
    <dbReference type="PhylomeDB" id="Q980F7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000449">
        <property type="entry name" value="30S ribosomal protein S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01474">
        <property type="entry name" value="Ribosomal_eS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027548">
        <property type="entry name" value="Ribosomal_eS19_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006811">
        <property type="entry name" value="PRK09333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000463548" description="Small ribosomal subunit protein eS19">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="10">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="158" mass="18118" checksum="19D3E49C3EA1413D" modified="2001-10-01" version="1">MSLIMITAEMVPPDLLIKRLAIYLKENVKTVDPPEWALLAKTASFKERVPDNAEDWWYIRAASLLRKLYVNSIIGIEKTRTIYGGRKRRGTRPEKFVKAPGHVNRLIFQQLEKAGLVQKIKNKGRSLSPKGRSLLDKLALEIFKELAENNTSLKVYLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q980H6</accession>
    <name>THYX_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flavin-dependent thymidylate synthase</fullName>
            <shortName evidence="1">FDTS</shortName>
            <ecNumber evidence="1">2.1.1.148</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">FAD-dependent thymidylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thymidylate synthase ThyX</fullName>
            <shortName evidence="1">TS</shortName>
            <shortName evidence="1">TSase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thyX</name>
        <name type="ordered locus">SSO0321</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH(2) as the reductant.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADPH + H(+) = dTMP + (6S)-5,6,7,8-tetrahydrofolate + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:29043"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.148"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidylate synthase ThyX family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.148" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90175">
        <property type="entry name" value="A90175"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990614.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980H6"/>
    <dbReference type="SMR" id="Q980H6"/>
    <dbReference type="FunCoup" id="Q980H6">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0321"/>
    <dbReference type="PaxDb" id="273057-SSO0321"/>
    <dbReference type="EnsemblBacteria" id="AAK40656">
        <property type="protein sequence ID" value="AAK40656"/>
        <property type="gene ID" value="SSO0321"/>
    </dbReference>
    <dbReference type="GeneID" id="1455466"/>
    <dbReference type="GeneID" id="44129295"/>
    <dbReference type="KEGG" id="sso:SSO0321"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.315"/>
    <dbReference type="eggNOG" id="arCOG01883">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077585_0_0_2"/>
    <dbReference type="InParanoid" id="Q980H6"/>
    <dbReference type="PhylomeDB" id="Q980H6"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050797">
        <property type="term" value="F:thymidylate synthase (FAD) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20175">
        <property type="entry name" value="ThyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.170:FF:000004">
        <property type="entry name" value="Flavin-dependent thymidylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01408">
        <property type="entry name" value="ThyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003669">
        <property type="entry name" value="Thymidylate_synthase_ThyX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036098">
        <property type="entry name" value="Thymidylate_synthase_ThyX_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02170">
        <property type="entry name" value="thyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34934">
        <property type="entry name" value="FLAVIN-DEPENDENT THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34934:SF1">
        <property type="entry name" value="FLAVIN-DEPENDENT THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02511">
        <property type="entry name" value="Thy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69796">
        <property type="entry name" value="Thymidylate synthase-complementing protein Thy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51331">
        <property type="entry name" value="THYX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000175597" description="Flavin-dependent thymidylate synthase">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="ThyX" evidence="2">
        <location>
            <begin position="2"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="ThyX motif" evidence="1">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="active site" description="Involved in ionization of N3 of dUMP, leading to its activation" evidence="1">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="77"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="80"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="88"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="158"/>
            <end position="160"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00661"/>
        </source>
    </evidence>
    <sequence length="260" mass="30568" checksum="684BDEFD04C665A0" modified="2001-10-01" version="1">MISVKLVSYTNDGEKVIAIAAKMSRSRKGWDYHEKDMTDDEIETWIRDAILHGYWSVLEHSVYTFSIEEISRVASHQLVRHRIASYTQMSHRFAKPIDEYYKPIIPPSAKKRSKELVEKAYKEAYDNYYTLLESGVPEEDARYVLPNGVNTNIVVTMNARELYNFFSLRLCSRAQWEIRAIAWKMLEEVKKVHPRLFKYTGPNCIIHENFIRNENESITLEDIFKDYKLEFISQRCIEGVLRDGIKKCIINSRSVLDNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q980K8</accession>
    <name>Q980K8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO5559</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90170">
        <property type="entry name" value="H90170"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990567.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980K8"/>
    <dbReference type="SMR" id="Q980K8"/>
    <dbReference type="STRING" id="273057.SSO5559"/>
    <dbReference type="PaxDb" id="273057-SSO5559"/>
    <dbReference type="EnsemblBacteria" id="AAK40623">
        <property type="protein sequence ID" value="AAK40623"/>
        <property type="gene ID" value="SSO5559"/>
    </dbReference>
    <dbReference type="GeneID" id="1455433"/>
    <dbReference type="KEGG" id="sso:SSO5559"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.279"/>
    <dbReference type="eggNOG" id="arCOG00540">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2565803_0_0_2"/>
    <dbReference type="InParanoid" id="Q980K8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17040">
        <property type="entry name" value="Ubl_MoaD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015221">
        <property type="entry name" value="Urm1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09138">
        <property type="entry name" value="Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="9675" checksum="81C02771B7CCC2D3" modified="2001-10-01" version="1">MPKVILKGPLISQFNFREIYVNDRELLRVLVKIDSKKHLILNESNQLKSGILILINGKDWRLYRNQLLNDNDIIEIIPINHGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q980P7</accession>
    <name>PURP_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase</fullName>
            <ecNumber evidence="2">6.3.4.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">purP</name>
        <name type="ordered locus">SSO0239</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + formate + ATP = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24836"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40581" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.3.4.23" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40581.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90165">
        <property type="entry name" value="F90165"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_014511515.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980P7"/>
    <dbReference type="SMR" id="Q980P7"/>
    <dbReference type="STRING" id="273057.SSO0239"/>
    <dbReference type="PaxDb" id="273057-SSO0239"/>
    <dbReference type="EnsemblBacteria" id="AAK40581">
        <property type="protein sequence ID" value="AAK40581"/>
        <property type="gene ID" value="SSO0239"/>
    </dbReference>
    <dbReference type="GeneID" id="1455391"/>
    <dbReference type="KEGG" id="sso:SSO0239"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.236"/>
    <dbReference type="eggNOG" id="arCOG04346">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065084_0_0_2"/>
    <dbReference type="InParanoid" id="Q980P7"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00134"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01163">
        <property type="entry name" value="IMP_biosynth_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023656">
        <property type="entry name" value="IMP_biosynth_PurP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009720">
        <property type="entry name" value="IMP_biosynth_PurP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010672">
        <property type="entry name" value="IMP_biosynth_PurP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009778">
        <property type="entry name" value="PRK13278.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147:SF2">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06849">
        <property type="entry name" value="DUF1246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06973">
        <property type="entry name" value="DUF1297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004602">
        <property type="entry name" value="ATPgrasp_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000348640" description="5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase">
        <location>
            <begin position="1"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="94"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="124"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="337" mass="38574" checksum="56CC5DC39444DB15" modified="2008-09-02" version="2">MIIATIGSHSALQILHGAKKEGFQTMVITDNKRENFYKNFSFIDNIYAYASLDEAVSIINGIKDYGILIPHGSLVEYLGKERVDKIETKIFGNKKIFEWEADQKKKMELLRKSNIKIPEIFERPEDVDRLVIVKLNGAKGGKGYFLARNKSEVKEGIQKLIESKMIRDENEVIIQEYVVGVPMYFQFFYSDIINRTEIFGIDIRYETNIDGLRRLPFEYMKELKINPTFVVVGNIPAVARESLLPVALEYANNFVRTTKELVPPGMIGPFCLESIITDNSDIVVFEFSGRIVAGTNLYVNGSPYSWLYWDEPMSIGRRIAREIRVANEKDKLSLVVS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Q7</accession>
    <name>NDK_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoside diphosphate kinase</fullName>
            <shortName evidence="1">NDK</shortName>
            <shortName evidence="1">NDP kinase</shortName>
            <ecNumber evidence="1">2.7.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-2-P kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ndk</name>
        <name type="ordered locus">SSO0230</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90164">
        <property type="entry name" value="D90164"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990482.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Q7"/>
    <dbReference type="SMR" id="Q980Q7"/>
    <dbReference type="FunCoup" id="Q980Q7">
        <property type="interactions" value="321"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0230"/>
    <dbReference type="PaxDb" id="273057-SSO0230"/>
    <dbReference type="EnsemblBacteria" id="AAK40571">
        <property type="protein sequence ID" value="AAK40571"/>
        <property type="gene ID" value="SSO0230"/>
    </dbReference>
    <dbReference type="GeneID" id="1455381"/>
    <dbReference type="GeneID" id="44129199"/>
    <dbReference type="KEGG" id="sso:SSO0230"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.226"/>
    <dbReference type="eggNOG" id="arCOG04313">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060216_6_3_2"/>
    <dbReference type="InParanoid" id="Q980Q7"/>
    <dbReference type="PhylomeDB" id="Q980Q7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000003">
        <property type="entry name" value="Nucleoside diphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000137102" description="Nucleoside diphosphate kinase">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00451"/>
        </source>
    </evidence>
    <sequence length="138" mass="15554" checksum="390714D857913B24" modified="2001-09-26" version="1">MVAQRTFVMIKPDGVKRGLIGEIISRFEKRGLKIVSLKMVKMSRDTAEKLYEEHKGKSFFEELVNYVTSGPVVCMVIEGDDVVQVIRRMIGNTDPKEAPPGTIRGDYALSKSENVIHASDSIEKAQREMSLFFDKSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q980S8</accession>
    <name>Q980S8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Small nuclear riboprotein protein (SnRNP-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">snRNP-1</name>
        <name evidence="3" type="ordered locus">SSO5410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90161">
        <property type="entry name" value="B90161"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990440.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980S8"/>
    <dbReference type="SMR" id="Q980S8"/>
    <dbReference type="FunCoup" id="Q980S8">
        <property type="interactions" value="139"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO5410"/>
    <dbReference type="PaxDb" id="273057-SSO5410"/>
    <dbReference type="EnsemblBacteria" id="AAK40545">
        <property type="protein sequence ID" value="AAK40545"/>
        <property type="gene ID" value="SSO5410"/>
    </dbReference>
    <dbReference type="GeneID" id="15298317"/>
    <dbReference type="KEGG" id="sso:SSO5410"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.199"/>
    <dbReference type="eggNOG" id="arCOG00998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_11_0_2"/>
    <dbReference type="InParanoid" id="Q980S8"/>
    <dbReference type="PhylomeDB" id="Q980S8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01726">
        <property type="entry name" value="LSm6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="2">
        <location>
            <begin position="7"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="87" mass="9788" checksum="19D9B0543DB3A61C" modified="2001-10-01" version="1">MQAKVENPLKSLRTAINRIVLVKLKDGSEYIGKLEQTDGTMNLVLRDCTEIREGTSEPVAKYGRVLIRGSNILFISVDYETVMNSEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-01-10" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q980T7</accession>
    <name>DCD_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">dCTP deaminase</fullName>
            <ecNumber evidence="1">3.5.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Deoxycytidine triphosphate deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dcd</name>
        <name type="ordered locus">SSO0190</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the deamination of dCTP to dUTP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dCTP + H2O + H(+) = dUTP + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="EC" id="3.5.4.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dCTP deaminase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40536" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.5.4.13" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40536.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90160">
        <property type="entry name" value="A90160"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_029552540.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980T7"/>
    <dbReference type="SMR" id="Q980T7"/>
    <dbReference type="FunCoup" id="Q980T7">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0190"/>
    <dbReference type="PaxDb" id="273057-SSO0190"/>
    <dbReference type="EnsemblBacteria" id="AAK40536">
        <property type="protein sequence ID" value="AAK40536"/>
        <property type="gene ID" value="SSO0190"/>
    </dbReference>
    <dbReference type="GeneID" id="1455346"/>
    <dbReference type="GeneID" id="44129159"/>
    <dbReference type="KEGG" id="sso:SSO0190"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.189"/>
    <dbReference type="eggNOG" id="arCOG04048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087476_3_0_2"/>
    <dbReference type="InParanoid" id="Q980T7"/>
    <dbReference type="PhylomeDB" id="Q980T7"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00665"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015949">
        <property type="term" value="P:nucleobase-containing small molecule interconversion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00146">
        <property type="entry name" value="dCTP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02274">
        <property type="entry name" value="dCTP_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680:SF3">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22769">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156040" description="dCTP deaminase">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="97"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="184" mass="21408" checksum="7F206F0D9C58E4D3" modified="2003-01-10" version="2">MILSDRDLKYYLEKSWIKIQPLREDTIRENGVDLRVGNEIARFKKTDKIFDPDNPDPSFFQTEKGEEFIIQPYEHVLLTTEEYIELNNDVMAFVNLRSTFARLGLFIPPTIVDAGFKGQVTIEVVGSSFPVKLKRSTRFIHLIFARTLTPVEYPYQGKYQGQKGVTLPKFNSQISSFYYQHQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q980V7</accession>
    <name>Q980V7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="4">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ilvB-1</name>
        <name evidence="8" type="ordered locus">SSO0170</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="4"/>
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        <property type="protein sequence ID" value="AAK40515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblBacteria" id="AAK40515">
        <property type="protein sequence ID" value="AAK40515"/>
        <property type="gene ID" value="SSO0170"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0170"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.166"/>
    <dbReference type="eggNOG" id="arCOG05937">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_502157_0_0_2"/>
    <dbReference type="InParanoid" id="Q980V7"/>
    <dbReference type="PhylomeDB" id="Q980V7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="7">
        <location>
            <begin position="43"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="6">
        <location>
            <begin position="231"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK40515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="571" mass="62805" checksum="DAF66658E23564B3" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q980V8</accession>
    <name>Q980V8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90157">
        <property type="entry name" value="C90157"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990393.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980V8"/>
    <dbReference type="STRING" id="273057.SSO0169"/>
    <dbReference type="PaxDb" id="273057-SSO0169"/>
    <dbReference type="EnsemblBacteria" id="AAK40514">
        <property type="protein sequence ID" value="AAK40514"/>
        <property type="gene ID" value="SSO0169"/>
    </dbReference>
    <dbReference type="GeneID" id="1453341"/>
    <dbReference type="KEGG" id="sso:SSO0169"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.165"/>
    <dbReference type="eggNOG" id="arCOG05944">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1500221_0_0_2"/>
    <dbReference type="InParanoid" id="Q980V8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="184" mass="19399" checksum="753EA1B7642A707F" modified="2001-10-01" version="1">MNDKSSSQISIRRAVIYAAVFSIASYAVAAGLALSTGLFAVNIFADPILNLTVPLILLSIGIQVINRKLGPIFIGLISAGLYAVSFLPFIAIPLIIIALIVEGVTRVMGYRSLKAVITYTAIAGGLEGVLSTLLALYMVKVPAPVITSAYIWTGVAVGMFAESALMGLISFYIVSYLIRAGVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q981D6</accession>
    <name>Q981D6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S-layer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90140">
        <property type="entry name" value="A90140"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981D6"/>
    <dbReference type="STRING" id="273057.SSO0011"/>
    <dbReference type="PaxDb" id="273057-SSO0011"/>
    <dbReference type="EnsemblBacteria" id="AAK40376">
        <property type="protein sequence ID" value="AAK40376"/>
        <property type="gene ID" value="SSO0011"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0011"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.11"/>
    <dbReference type="eggNOG" id="arCOG02089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_335136_0_0_2"/>
    <dbReference type="InParanoid" id="Q981D6"/>
    <dbReference type="PhylomeDB" id="Q981D6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902:SF3">
        <property type="entry name" value="NPCBM-ASSOCIATED, NEW3 DOMAIN OF ALPHA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902">
        <property type="entry name" value="S-LAYER DOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="947"/>
            <end position="967"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="974" mass="105203" checksum="A74B5ED8581070F9" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q981E0</accession>
    <name>Q981E0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamine amidotransferase type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0007</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90139">
        <property type="entry name" value="E90139"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989646.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981E0"/>
    <dbReference type="STRING" id="273057.SSO0007"/>
    <dbReference type="PaxDb" id="273057-SSO0007"/>
    <dbReference type="EnsemblBacteria" id="AAK40372">
        <property type="protein sequence ID" value="AAK40372"/>
        <property type="gene ID" value="SSO0007"/>
    </dbReference>
    <dbReference type="GeneID" id="1455262"/>
    <dbReference type="KEGG" id="sso:SSO0007"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.7"/>
    <dbReference type="eggNOG" id="arCOG03639">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086262_0_0_2"/>
    <dbReference type="InParanoid" id="Q981E0"/>
    <dbReference type="PhylomeDB" id="Q981E0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42824">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42824:SF1">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YAFJ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13230">
        <property type="entry name" value="GATase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="240" mass="27932" checksum="C476B350756DA159" modified="2001-10-01" version="1">MCRILAFHTKGEISKEYVNALIRASRNDIFSKYGSHPDGWGLSIFLKRNDKWKVIYYRSEDPIYEDPNISYLIDIAKGEDIVGVIHARKAGRKFLTGLSHAHPYYMRANIYDLYFAHNGSVSRTSFKDSNRPFTDSYLILEEIKTLIESNMSPFDAYAITLDKLKDCSTSLNSSLIYYNKKEGPSILIGYYYNRNRLSKETNEEYYKLYTDNKRYIFSSTIKYYLGVDAEELIMGSIIHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q981E2</accession>
    <name>Q981E2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribokinase</fullName>
            <shortName evidence="10">RK</shortName>
            <ecNumber evidence="10">2.7.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rbsK-1</name>
        <name evidence="10" type="synonym">rbsK</name>
        <name evidence="12" type="ordered locus">SSO0004</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-ribose + ATP = D-ribose 5-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13697"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:47013"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase pfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.15" evidence="10"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90139">
        <property type="entry name" value="C90139"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989648.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981E2"/>
    <dbReference type="FunCoup" id="Q981E2">
        <property type="interactions" value="269"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0004"/>
    <dbReference type="PaxDb" id="273057-SSO0004"/>
    <dbReference type="EnsemblBacteria" id="AAK40370">
        <property type="protein sequence ID" value="AAK40370"/>
        <property type="gene ID" value="SSO0004"/>
    </dbReference>
    <dbReference type="GeneID" id="1455260"/>
    <dbReference type="GeneID" id="44128966"/>
    <dbReference type="KEGG" id="sso:SSO0004"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.4"/>
    <dbReference type="eggNOG" id="arCOG00014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_2_2_2"/>
    <dbReference type="InParanoid" id="Q981E2"/>
    <dbReference type="PhylomeDB" id="Q981E2"/>
    <dbReference type="UniPathway" id="UPA00916">
        <property type="reaction ID" value="UER00889"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019206">
        <property type="term" value="F:nucleoside kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004747">
        <property type="term" value="F:ribokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019303">
        <property type="term" value="P:D-ribose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01174">
        <property type="entry name" value="ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000053">
        <property type="entry name" value="Ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01987">
        <property type="entry name" value="Ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011877">
        <property type="entry name" value="Ribokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02152">
        <property type="entry name" value="D_ribokin_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584:SF166">
        <property type="entry name" value="RIBOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="11">
        <location>
            <begin position="2"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="37"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="206"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="238"/>
            <end position="239"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK40370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="293" mass="31836" checksum="9B1FC90D2C02B32A" modified="2001-10-01" version="1">MIIVVGSYNVDVILKVNKIPEIGETVIADELYITHGGKGSNQAVSASRLGSHVKIIVAVGDDKHGTDAIEFWKSENIDVSKVKVKSGINTGTAYIFVDKKGRNIIVVNRGANYYLSEDDLNNSLEGDILLMQLEIRESVVKKAAKEFNGIKILNPAPANLKDTEILSLVDIITPNEIEFKELVNTDDFEYGLNLLLKKVKKAVIVTLGERGALLATRDGKKVLIPAPKVNVIDETGAGDVFNAALSVYLEKGYDLETAVEYANKVAALSVTKIGALGPKLDEVVRFLEEIDKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-20" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q9V2T8</accession>
    <name>THSB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chaperonin subunit beta</fullName>
            <ecNumber evidence="1">5.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Thermophilic factor 55 beta</fullName>
            <shortName evidence="6">TF55-beta</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Thermosome subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">thsB</name>
        <name type="ordered locus">SSO0282</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="Curr. Biol." volume="9" first="1053" last="1056">
            <title>Recurrent paralogy in the evolution of archaeal chaperonins.</title>
            <authorList>
                <person name="Archibald J.M."/>
                <person name="Logsdon J.M. Jr."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="10508614"/>
            <dbReference type="DOI" id="10.1016/S0960-9822(99)80457-6"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 103-524</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="8 9 10" key="3">
        <citation type="journal article" date="2016" name="Structure" volume="24" first="364" last="374">
            <title>Structural and Functional Insights into the Evolution and Stress Adaptation of Type II Chaperonins.</title>
            <authorList>
                <person name="Chaston J.J."/>
                <person name="Smits C."/>
                <person name="Aragao D."/>
                <person name="Wong A.S."/>
                <person name="Ahsan B."/>
                <person name="Sandin S."/>
                <person name="Molugu S.K."/>
                <person name="Molugu S.K."/>
                <person name="Bernal R.A."/>
                <person name="Stock D."/>
                <person name="Stewart A.G."/>
            </authorList>
            <dbReference type="PubMed" id="26853941"/>
            <dbReference type="DOI" id="10.1016/j.str.2015.12.016"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) IN COMPLEX WITH ADP</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="4">
        <citation type="journal article" date="2021" name="Acta Crystallogr. F Struct. Biol. Commun." volume="77" first="79" last="84">
            <title>Structural analysis of the Sulfolobus solfataricus TF55beta chaperonin by cryo-electron microscopy.</title>
            <authorList>
                <person name="Zeng Y.C."/>
                <person name="Sobti M."/>
                <person name="Stewart A.G."/>
            </authorList>
            <dbReference type="PubMed" id="33682792"/>
            <dbReference type="DOI" id="10.1107/s2053230x21002223"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (3.62 ANGSTROMS) IN COMPLEX WITH ADP; ATP OR MG(2+)</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; binds unfolded polypeptides and stimulates protein folding in a nucleotide-hydrolysis-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + an unfolded polypeptide = ADP + phosphate + a folded polypeptide.</text>
            <dbReference type="EC" id="5.6.1.7"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text evidence="3">Thermostable, it unfolds at 94 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="3 4">The 3 paralogous subunits (alpha, beta and gamma) form oligomeric ring complexes, composition is temperature dependent (PubMed:26853941). Gamma is unstable at 75 degrees Celsius, at 80 degrees Celsius in vivo there are 94-95% alpha-beta complexes with 5-6% beta-only rings, and no detectable gamma-containing rings (PubMed:26853941). In the beta subunit-only complex 2 rings of 9 subunits each stack back-to-back to form an octadecameric complex (PubMed:26853941, PubMed:33682792). In the mixed alpha-beta complex there are 2 stacked 8-membered rings with alternating alpha and beta subunits making a hexadecameric complex; in the stacked rings alpha subunits stack on the beta subunits and only the beta subunits bind ADP (PubMed:26853941). This subunit forms complexes by itself, with the alpha subunit or the alpha and gamma subunits in the presence of ATP; the gamma-containing complexes have 2 rings of 9 subunits making an octadecameric complex (PubMed:26853941).</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-16195251">
            <id>Q9V2T8</id>
        </interactant>
        <interactant intactId="EBI-16195227">
            <id>Q9V2S9</id>
            <label>thsA</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>7</experiments>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-16195251">
            <id>Q9V2T8</id>
        </interactant>
        <interactant intactId="EBI-16195251">
            <id>Q9V2T8</id>
            <label>thsB</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>4</experiments>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="sequence caution" evidence="7">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40620" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.7" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40620.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF149920">
        <property type="protein sequence ID" value="AAF03361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90170">
        <property type="entry name" value="E90170"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990564.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="4XCD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.79 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-554"/>
    </dbReference>
    <dbReference type="PDB" id="4XCG">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.74 A"/>
        <property type="chains" value="B=1-554"/>
    </dbReference>
    <dbReference type="PDB" id="4XCI">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="B=1-554"/>
    </dbReference>
    <dbReference type="PDB" id="6XHI">
        <property type="method" value="EM"/>
        <property type="resolution" value="4.19 A"/>
        <property type="chains" value="A=1-554"/>
    </dbReference>
    <dbReference type="PDB" id="6XHJ">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.62 A"/>
        <property type="chains" value="A=1-554"/>
    </dbReference>
    <dbReference type="PDBsum" id="4XCD"/>
    <dbReference type="PDBsum" id="4XCG"/>
    <dbReference type="PDBsum" id="4XCI"/>
    <dbReference type="PDBsum" id="6XHI"/>
    <dbReference type="PDBsum" id="6XHJ"/>
    <dbReference type="AlphaFoldDB" id="Q9V2T8"/>
    <dbReference type="EMDB" id="EMD-22186"/>
    <dbReference type="EMDB" id="EMD-22187"/>
    <dbReference type="SMR" id="Q9V2T8"/>
    <dbReference type="DIP" id="DIP-61907N"/>
    <dbReference type="FunCoup" id="Q9V2T8">
        <property type="interactions" value="269"/>
    </dbReference>
    <dbReference type="IntAct" id="Q9V2T8">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0282"/>
    <dbReference type="PaxDb" id="273057-SSO0282"/>
    <dbReference type="EnsemblBacteria" id="AAK40620">
        <property type="protein sequence ID" value="AAK40620"/>
        <property type="gene ID" value="SSO0282"/>
    </dbReference>
    <dbReference type="GeneID" id="1455430"/>
    <dbReference type="GeneID" id="44129253"/>
    <dbReference type="KEGG" id="sso:SSO0282"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.276"/>
    <dbReference type="eggNOG" id="arCOG01257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_2"/>
    <dbReference type="InParanoid" id="Q9V2T8"/>
    <dbReference type="PhylomeDB" id="Q9V2T8"/>
    <dbReference type="EvolutionaryTrace" id="Q9V2T8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000353"/>
        <property type="project" value="IntAct"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000014">
        <property type="entry name" value="Thermosome subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000128402" description="Chaperonin subunit beta">
        <location>
            <begin position="1"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="532"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="540"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 12">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 12">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 8">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 8">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="503"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="503"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAF03361." evidence="7" ref="2">
        <original>I</original>
        <variation>M</variation>
        <location>
            <position position="298"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="55"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="turn" evidence="13">
        <location>
            <begin position="61"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="64"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="71"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="turn" evidence="13">
        <location>
            <begin position="83"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="86"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="95"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="turn" evidence="13">
        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="turn" evidence="13">
        <location>
            <begin position="168"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="193"/>
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    <feature type="strand" evidence="13">
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    <feature type="turn" evidence="13">
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    <feature type="helix" evidence="13">
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    <feature type="helix" evidence="13">
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    <feature type="helix" evidence="13">
        <location>
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    <feature type="helix" evidence="13">
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    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q6LX38"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="26853941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="33682792"/>
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        <source>
            <dbReference type="PubMed" id="10508614"/>
        </source>
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        <source>
            <dbReference type="PubMed" id="26853941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="4XCD"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="4XCG"/>
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    <evidence type="ECO:0007744" key="10">
        <source>
            <dbReference type="PDB" id="4XCI"/>
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        <source>
            <dbReference type="PDB" id="6XHI"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="12">
        <source>
            <dbReference type="PDB" id="6XHJ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="13">
        <source>
            <dbReference type="PDB" id="4XCI"/>
        </source>
    </evidence>
    <sequence length="554" mass="59952" checksum="927BF96B78A59547" modified="2001-06-20" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-02-21" modified="2025-06-18" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q9Y8K8</accession>
    <name>UBIX_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flavin prenyltransferase UbiX</fullName>
            <ecNumber evidence="1">2.5.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ubiX</name>
        <name type="ordered locus">SSO0437</name>
        <name type="ORF">C41_048</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for 4-hydroxy-3-polyprenylbenzoic acid decarboxylase UbiD. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dimethylallyl phosphate + FMNH2 = prenylated FMNH2 + phosphate</text>
            <dbReference type="Rhea" id="RHEA:37743"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:87467"/>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
            <dbReference type="EC" id="2.5.1.129"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiX/PAD1 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40762" version="1"/>
        </conflict>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAB46087" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.5.1.129" evidence="1"/>
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        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40762.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90188">
        <property type="entry name" value="C90188"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9Y8K8"/>
    <dbReference type="SMR" id="Q9Y8K8"/>
    <dbReference type="FunCoup" id="Q9Y8K8">
        <property type="interactions" value="139"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0437"/>
    <dbReference type="PaxDb" id="273057-SSO0437"/>
    <dbReference type="EnsemblBacteria" id="AAK40762">
        <property type="protein sequence ID" value="AAK40762"/>
        <property type="gene ID" value="SSO0437"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0437"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.430"/>
    <dbReference type="eggNOG" id="arCOG01703">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074522_0_1_2"/>
    <dbReference type="InParanoid" id="Q9Y8K8"/>
    <dbReference type="PhylomeDB" id="Q9Y8K8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106141">
        <property type="term" value="F:flavin prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1950:FF:000012">
        <property type="entry name" value="Flavin prenyltransferase UbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01984">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004507">
        <property type="entry name" value="UbiX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004685">
        <property type="entry name" value="PRK06029.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00421">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43374">
        <property type="entry name" value="FLAVIN PRENYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43374:SF1">
        <property type="entry name" value="FLAVIN PRENYLTRANSFERASE PAD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0637">Prenyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000134982" description="Flavin prenyltransferase UbiX">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="23"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="103"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="201" mass="22284" checksum="055DCF6151B16F7E" modified="2001-02-21" version="2">MVKEAGTKNRREKTKRIIIGISGASGTIYGIRTVQFLNELGYEIHIIISKSAEKVAQKELGINLINELKKYSSYIYNQSQIEASPSSSSFSITSKGMIIIPCSIKTLAEIANGIGSNLLSRTALNFIRTNKRLVLVIRETPLGAIELENALKLARLGVYIMPASPAFYILPKNIDDMINFIVGKALDLLGIKHDIYKRWKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1994-02-01" modified="2026-01-28" version="160" xmlns="https://uniprot.org/uniprot">
    <accession>P35021</accession>
    <name>EF1A_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor 1-alpha</fullName>
            <shortName evidence="2">EF-1-alpha</shortName>
            <ecNumber evidence="2">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Elongation factor Tu</fullName>
            <shortName evidence="2">EF-Tu</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tuf</name>
        <name type="synonym">tef1</name>
        <name type="ordered locus">SSO0216</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1993" name="Nucleic Acids Res." volume="21" first="1666" last="1666">
            <title>Primary structure of the elongation factor 1 alpha in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Arcari P."/>
                <person name="Gallo M."/>
                <person name="Ianniciello G."/>
                <person name="Dello Russo A."/>
                <person name="Bocchini V."/>
            </authorList>
            <dbReference type="PubMed" id="8369039"/>
            <dbReference type="DOI" id="10.1093/nar/21.7.1666"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1994" name="Biochim. Biophys. Acta" volume="1217" first="333" last="337">
            <title>The nucleotide sequence of the gene coding for the elongation factor 1 alpha in Sulfolobus solfataricus. Homology of the product with related proteins.</title>
            <authorList>
                <person name="Arcari P."/>
                <person name="Gallo M."/>
                <person name="Ianniciello G."/>
                <person name="Dello Russo A."/>
                <person name="Bocchini V."/>
            </authorList>
            <dbReference type="PubMed" id="8148382"/>
            <dbReference type="DOI" id="10.1016/0167-4781(94)90296-8"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="submission" date="2001-06" db="EMBL/GenBank/DDBJ databases">
            <title>The nucleotide sequence of the gene encoding the elongation factor 1 alpha from the archaeon Sulfolobus solfataricus isolate MT3.</title>
            <authorList>
                <person name="Arcari P."/>
                <person name="Masullo M."/>
                <person name="Bocchini V."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>MT-3</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">GTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
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    <sequence length="435" mass="48489" checksum="222B540A31768A59" modified="2001-06-01" version="3">MSQKPHLNLIVIGHIDHGKSTLVGRLLMDRGFIDEKTVKEAEEAAKKLGKESEKFAFLLDRLKEERERGVTINLTFMRFETKKYFFTIIDAPGHRDFVKNMITGASQADAAILVVSAKKGEYEAGMSVEGQTREHIILAKTMGLDQLIVAVNKMDLTEPPYDEKRYKEIVDQVSKFMRSYGFNTNKVRFVPVVAPAGDNITHRSENMKWYNGPTLEEYLDQLELPPKPVDKPLRIPIQDVYSISGVGTVPVGRVESGVLKVGDKIVFMPAGKVGEVRSIETHHTKMDKAEPGDNIGFNVRGVEKKDIKRGDVVGHPNNPPTVADEFTARIIVVWHPTALANGYTPVIHVHTASVACRVSELVSKLDPRTGQEAEKNPQFLKQGDVAIVKFKPIKPLCVEKYNEFPPLGRFAMRDMGKTVGVGIIVDVKPAKVEIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2026-01-28" version="148" xmlns="https://uniprot.org/uniprot">
    <accession>P50252</accession>
    <accession>Q9UXE3</accession>
    <name>SAHH_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="1">3.13.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">S-adenosyl-L-homocysteine hydrolase</fullName>
            <shortName evidence="1">AdoHcyase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ahcY</name>
        <name type="ordered locus">SSO0755</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Gene" volume="177" first="17" last="22">
            <title>Cloning and sequencing of the gene coding for S-adenosylhomocysteine hydrolase in the thermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Porcelli M."/>
                <person name="Cacciapuoti G."/>
                <person name="Fusco S."/>
                <person name="Bertoldo C."/>
                <person name="de Rosa M."/>
                <person name="Zappia V."/>
            </authorList>
            <dbReference type="PubMed" id="8921839"/>
            <dbReference type="DOI" id="10.1016/0378-1119(96)00263-6"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
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            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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    </reference>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
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            <text>S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine</text>
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    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="417"/>
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<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="146" xmlns="https://uniprot.org/uniprot">
    <accession>P55858</accession>
    <name>RL7A_SACS2</name>
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        <recommendedName>
            <fullName evidence="2 10">Large ribosomal subunit protein eL8</fullName>
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        <alternativeName>
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    <gene>
        <name evidence="2 6" type="primary">rpl7ae</name>
        <name type="ordered locus">SSO0091</name>
        <name evidence="8" type="ORF">C04_031</name>
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    <organism>
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        <name type="synonym">Sulfolobus solfataricus</name>
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            <taxon>Archaea</taxon>
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            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
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                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
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            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
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        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
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            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2009" name="Nucleic Acids Res." volume="37" first="256" last="267">
            <title>Function and ribosomal localization of aIF6, a translational regulator shared by archaea and eukarya.</title>
            <authorList>
                <person name="Benelli D."/>
                <person name="Marzi S."/>
                <person name="Mancone C."/>
                <person name="Alonzi T."/>
                <person name="la Teana A."/>
                <person name="Londei P."/>
            </authorList>
            <dbReference type="PubMed" id="19036786"/>
            <dbReference type="DOI" id="10.1093/nar/gkn959"/>
        </citation>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>INDUCTION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2009" name="Proc. Natl. Acad. Sci. U.S.A." volume="106" first="13808" last="13813">
            <title>Structural organization of box C/D RNA-guided RNA methyltransferase.</title>
            <authorList>
                <person name="Ye K."/>
                <person name="Jia R."/>
                <person name="Lin J."/>
                <person name="Ju M."/>
                <person name="Peng J."/>
                <person name="Xu A."/>
                <person name="Zhang L."/>
            </authorList>
            <dbReference type="PubMed" id="19666563"/>
            <dbReference type="DOI" id="10.1073/pnas.0905128106"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (4.01 ANGSTROMS) IN COMPLEX WITH RNA; S-NOP5 AND FLPA</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference evidence="11 12 13" key="5">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 4">Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D (PubMed:19666563) and box C'/D' sRNAs.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 3 4 5">Part of the 30 (PubMed:19036786, PubMed:39753558) and 50S (PubMed:19036786) ribosomal subunit. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA (PubMed:19666563). These sRNP particles form homodimers, giving rise to an asymmetric holoenzyme (PubMed:19666563). Probably part of the RNase P complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 3">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="3 5">Found free in the cytoplasm and associated with 30S (PubMed:19036786, PubMed:39753558) and 50S (PubMed:19036786) ribosomal subunits (PubMed:19036786).</text>
    </comment>
    <comment type="induction">
        <text evidence="3">Constitutuively expressed (at protein level).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="sequence caution" evidence="9">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40449" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <comment type="sequence caution" evidence="9">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAA69560" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69560.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40449.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75397">
        <property type="entry name" value="S75397"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988903.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3ID5">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="4.01 A"/>
        <property type="chains" value="C/G=1-127"/>
    </dbReference>
    <dbReference type="PDB" id="5GIN">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.31 A"/>
        <property type="chains" value="C/D/L=3-127"/>
    </dbReference>
    <dbReference type="PDB" id="5GIO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.60 A"/>
        <property type="chains" value="C/D/L=3-127"/>
    </dbReference>
    <dbReference type="PDB" id="5GIP">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.13 A"/>
        <property type="chains" value="C/D/M/N=3-127"/>
    </dbReference>
    <dbReference type="PDB" id="5JPQ">
        <property type="method" value="EM"/>
        <property type="resolution" value="7.30 A"/>
        <property type="chains" value="U/V=1-127"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="3=1-127"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="3=1-127"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="3=1-127"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="3=1-127"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="3=1-127"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="3=1-127"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="3=1-127"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="3=1-127"/>
    </dbReference>
    <dbReference type="PDBsum" id="3ID5"/>
    <dbReference type="PDBsum" id="5GIN"/>
    <dbReference type="PDBsum" id="5GIO"/>
    <dbReference type="PDBsum" id="5GIP"/>
    <dbReference type="PDBsum" id="5JPQ"/>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="P55858"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="P55858"/>
    <dbReference type="DIP" id="DIP-48940N"/>
    <dbReference type="FunCoup" id="P55858">
        <property type="interactions" value="231"/>
    </dbReference>
    <dbReference type="IntAct" id="P55858">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0091"/>
    <dbReference type="PaxDb" id="273057-SSO0091"/>
    <dbReference type="EnsemblBacteria" id="AAK40449">
        <property type="protein sequence ID" value="AAK40449"/>
        <property type="gene ID" value="SSO0091"/>
    </dbReference>
    <dbReference type="GeneID" id="42362205"/>
    <dbReference type="GeneID" id="44129051"/>
    <dbReference type="KEGG" id="sso:SSO0091"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.89"/>
    <dbReference type="eggNOG" id="arCOG01751">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084513_4_0_2"/>
    <dbReference type="InParanoid" id="P55858"/>
    <dbReference type="PhylomeDB" id="P55858"/>
    <dbReference type="EvolutionaryTrace" id="P55858"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000020">
        <property type="entry name" value="50S ribosomal protein L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00326">
        <property type="entry name" value="Ribosomal_eL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022481">
        <property type="entry name" value="Ribosomal_eL8_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03677">
        <property type="entry name" value="eL8_ribo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00884">
        <property type="entry name" value="RIBOSOMALHS6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000136806" description="Large ribosomal subunit protein eL8">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="helix" evidence="14">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="strand" evidence="14">
        <location>
            <begin position="30"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="helix" evidence="14">
        <location>
            <begin position="36"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="strand" evidence="14">
        <location>
            <begin position="49"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="helix" evidence="14">
        <location>
            <begin position="60"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="helix" evidence="14">
        <location>
            <begin position="66"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="strand" evidence="14">
        <location>
            <begin position="77"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="helix" evidence="14">
        <location>
            <begin position="83"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="strand" evidence="14">
        <location>
            <begin position="97"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="helix" evidence="14">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P62426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="19036786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="19666563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="6">
        <source>
            <dbReference type="PubMed" id="11427726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="7">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="8">
        <source>
            <dbReference type="PubMed" id="8899719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="9"/>
    <evidence type="ECO:0000312" key="10">
        <source>
            <dbReference type="EMBL" id="CAA69560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="11">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="12">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="13">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="14">
        <source>
            <dbReference type="PDB" id="5GIP"/>
        </source>
    </evidence>
    <sequence length="127" mass="13736" checksum="3EE30FC81C1A003D" modified="2001-04-27" version="2">MSKASYVKFEVPQDLADKVLEAVRKAKESGKIKKGTNETTKAVERGQAKLVIIAEDVQPEEIVAHLPLLCDEKKIPYVYVSSKKALGEACGLQVATASAAILEPGEAKDLVDEIIKRVNEIKGKTSS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-20" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>P58179</accession>
    <name>PFDA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Prefoldin subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>GimC subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pfdA</name>
        <name type="ordered locus">SSO0349</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90177">
        <property type="entry name" value="H90177"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990641.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58179"/>
    <dbReference type="SMR" id="P58179"/>
    <dbReference type="FunCoup" id="P58179">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0349"/>
    <dbReference type="PaxDb" id="273057-SSO0349"/>
    <dbReference type="EnsemblBacteria" id="AAK40679">
        <property type="protein sequence ID" value="AAK40679"/>
        <property type="gene ID" value="SSO0349"/>
    </dbReference>
    <dbReference type="GeneID" id="1455489"/>
    <dbReference type="GeneID" id="44129320"/>
    <dbReference type="KEGG" id="sso:SSO0349"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.340"/>
    <dbReference type="eggNOG" id="arCOG01341">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1792160_0_0_2"/>
    <dbReference type="InParanoid" id="P58179"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00584">
        <property type="entry name" value="Prefoldin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00308">
        <property type="entry name" value="PfdA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PFD_alpha_archaea"/>
    </dbReference>
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        <property type="entry name" value="Prefoldin"/>
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        <property type="entry name" value="Prefoldin_subunit_alpha"/>
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        <property type="entry name" value="prefoldin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674:SF2">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000153688" description="Prefoldin subunit alpha">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="147" mass="16330" checksum="6022B2698B2B8D65" modified="2001-06-20" version="1">MSQGQGGITLDELIAQADYLKRYIDSLQRTQLELLESINSIDSAKQAIETMKSGNKEMLVFIDRKGYLLAKVGGIIGDKVTVHLGLSYYAEVDLDSAIKILDKRKDEISKAAQDLNNELQKAASTYNQIVDILNQIQQAAARRQQGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>P95994</accession>
    <name>Y066_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MEMO1 family protein SSO0066</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0066</name>
        <name type="ORF">C05005</name>
        <name type="ORF">C05_044</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MEMO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75422">
        <property type="entry name" value="S75422"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988870.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95994"/>
    <dbReference type="SMR" id="P95994"/>
    <dbReference type="FunCoup" id="P95994">
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    </dbReference>
    <dbReference type="STRING" id="273057.SSO0066"/>
    <dbReference type="PaxDb" id="273057-SSO0066"/>
    <dbReference type="EnsemblBacteria" id="AAK40427">
        <property type="protein sequence ID" value="AAK40427"/>
        <property type="gene ID" value="SSO0066"/>
    </dbReference>
    <dbReference type="GeneID" id="1455318"/>
    <dbReference type="KEGG" id="sso:SSO0066"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.66"/>
    <dbReference type="eggNOG" id="arCOG01728">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038085_2_0_2"/>
    <dbReference type="InParanoid" id="P95994"/>
    <dbReference type="PhylomeDB" id="P95994"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROTEIN MEMO1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060:SF0">
        <property type="entry name" value="PROTEIN MEMO1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF53213">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00055"/>
        </source>
    </evidence>
    <sequence length="284" mass="32192" checksum="C9A554BC7AD01D2E" modified="1997-05-01" version="1">MRRLPAVAGSFYESDPKKLKMQIEWSFRHNIGPRDIPKQSYEKKKRDNLFFIVPHAGYIYSGPVAAHSYYYLASEGKPDVVIILGPNHTGLGSYVSAWPKGEWETPLGSVKVDEEVLMQLVMESEVIDLEEKSHLYEHSIEVQLPFLQYFFDDNFKIVPIVIMMQTPEIAEFLADAIYKVIQKYSDKDIVVLASSDMNHYDPHEITMKKDEEAIEKIQQLDYRGLYEVVEGKDVTLCGYGPIMVSLILAKKLGKKAYILKHATSGDTSGPKDSVVGYLAARFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX72</accession>
    <name>Q7LX72_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">L-lactate permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2126</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="S74038"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX72"/>
    <dbReference type="STRING" id="273057.SSO2126"/>
    <dbReference type="PaxDb" id="273057-SSO2126"/>
    <dbReference type="EnsemblBacteria" id="AAK42305">
        <property type="protein sequence ID" value="AAK42305"/>
        <property type="gene ID" value="SSO2126"/>
    </dbReference>
    <dbReference type="GeneID" id="1453632"/>
    <dbReference type="KEGG" id="sso:SSO2126"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2218"/>
    <dbReference type="eggNOG" id="arCOG04386">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021628_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX72"/>
    <dbReference type="PhylomeDB" id="Q7LX72"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015129">
        <property type="term" value="F:lactate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015295">
        <property type="term" value="F:solute:proton symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003804">
        <property type="entry name" value="Lactate_perm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00795">
        <property type="entry name" value="lctP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30003:SF0">
        <property type="entry name" value="GLYCOLATE PERMEASE GLCA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30003">
        <property type="entry name" value="L-LACTATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02652">
        <property type="entry name" value="Lactate_perm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="530" mass="57225" checksum="E700D77EAEDF8446" modified="2011-05-31" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXE2</accession>
    <name>Q7LXE2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polysaccharide biosynthesis related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90234">
        <property type="entry name" value="D90234"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923126.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXE2"/>
    <dbReference type="STRING" id="273057.SSO0837"/>
    <dbReference type="PaxDb" id="273057-SSO0837"/>
    <dbReference type="EnsemblBacteria" id="AAK41131">
        <property type="protein sequence ID" value="AAK41131"/>
        <property type="gene ID" value="SSO0837"/>
    </dbReference>
    <dbReference type="GeneID" id="1455098"/>
    <dbReference type="KEGG" id="sso:SSO0837"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.851"/>
    <dbReference type="eggNOG" id="arCOG02209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046380_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXE2"/>
    <dbReference type="PhylomeDB" id="Q7LXE2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052556">
        <property type="entry name" value="PolySynth_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43424">
        <property type="entry name" value="LOCUS PUTATIVE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43424:SF1">
        <property type="entry name" value="LOCUS PUTATIVE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="403"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="424"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="469" mass="52049" checksum="AA28DD184830FD00" modified="2011-05-31" version="1">MNPIKNALKSLSVITTNVLIALIFFIVTAKISNPAFFGKVAIIQLLEVVTFTFFYFIPGQIITREISYLYAKKEVNKGVVGKFLSFPFLAIPVFLIFLLFPNYVKLAIPYLFLYLLNNVLVAIMIGMDMFTETAITGNLFLIIRWGISIIAVLSHNIYLFVEIWILGGVLSVLLNYSFLSRKVGLVLPSLDFAFLFKHFKEGLPLYLSSSANFLSSQGDRVTTAYLLGSYYLGLYQFSALVAGVPAMVLGALNNVLLPTASFYKALGSDEKKMSSISFRVLALISFLSVIISIPIAILVVERFFPDYTEGIRVLILLLLATTLPFPINSLTNFIIAAKKSLRPFLFLSLLNAGVVLLTSFLLIPRIGIMGGAISQVIVTVISSSFILVYALRTSTFILGKREGILLTAMPLIALYEVFVDPPYLDLLLLLSVIVLFKLFKIISTEEVRLIESFLPSKLKFIASLLKVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXJ4</accession>
    <name>Q7LXJ4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA repair protein radA homolog (RadA-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">radA-like</name>
        <name evidence="2" type="ordered locus">SSO0777</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90227">
        <property type="entry name" value="C90227"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923069.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXJ4"/>
    <dbReference type="STRING" id="273057.SSO0777"/>
    <dbReference type="PaxDb" id="273057-SSO0777"/>
    <dbReference type="EnsemblBacteria" id="AAK41074">
        <property type="protein sequence ID" value="AAK41074"/>
        <property type="gene ID" value="SSO0777"/>
    </dbReference>
    <dbReference type="GeneID" id="7806743"/>
    <dbReference type="KEGG" id="sso:SSO0777"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.778"/>
    <dbReference type="eggNOG" id="arCOG00417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1471995_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXJ4"/>
    <dbReference type="PhylomeDB" id="Q7LXJ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01874">
        <property type="entry name" value="DNAREPAIRADA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="175" mass="19798" checksum="BDAC77239C25C8A4" modified="2011-05-31" version="1">MNLSTFVFERGNLVSIYGESGVGKTSLSLELALEIRTSVFISTEGSLFEARLEKIKVGQGVYFASVKSNIELFNGIINSLEYKPSLIVVDTINTFYRYERNVHSFLKLLIILRSIAQSNVKILLVWEVSANNKVAGEKFMRKFSDDVLRITKSYIIGNLRRCKFKITERGVIGCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXP1</accession>
    <name>Q7LXP1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycerate kinase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0666</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90214">
        <property type="entry name" value="C90214"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991211.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXP1"/>
    <dbReference type="FunCoup" id="Q7LXP1">
        <property type="interactions" value="202"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0666"/>
    <dbReference type="PaxDb" id="273057-SSO0666"/>
    <dbReference type="EnsemblBacteria" id="AAK40970">
        <property type="protein sequence ID" value="AAK40970"/>
        <property type="gene ID" value="SSO0666"/>
    </dbReference>
    <dbReference type="GeneID" id="1454937"/>
    <dbReference type="KEGG" id="sso:SSO0666"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.667"/>
    <dbReference type="eggNOG" id="arCOG04170">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032279_1_1_2"/>
    <dbReference type="InParanoid" id="Q7LXP1"/>
    <dbReference type="PhylomeDB" id="Q7LXP1"/>
    <dbReference type="BRENDA" id="2.7.1.165">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008887">
        <property type="term" value="F:glycerate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10180:FF:000005">
        <property type="entry name" value="Glycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1480.10:FF:000005">
        <property type="entry name" value="Glycerate kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10180">
        <property type="entry name" value="Glycerate kinase, MOFRL-like N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1480.10">
        <property type="entry name" value="MOFRL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037035">
        <property type="entry name" value="GK-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038614">
        <property type="entry name" value="GK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053656">
        <property type="entry name" value="Glycerate_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007835">
        <property type="entry name" value="MOFRL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025286">
        <property type="entry name" value="MOFRL_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039760">
        <property type="entry name" value="MOFRL_protein"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041176">
        <property type="entry name" value="GlyK_Thmprot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12227">
        <property type="entry name" value="GLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12227:SF0">
        <property type="entry name" value="GLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13660">
        <property type="entry name" value="DUF4147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05161">
        <property type="entry name" value="MOFRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82544">
        <property type="entry name" value="GckA/TtuD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MOFRL-associated" evidence="2">
        <location>
            <begin position="2"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="MOFRL" evidence="1">
        <location>
            <begin position="292"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="400" mass="44415" checksum="027BBA503C9AF392" modified="2011-05-31" version="1">MDIVDKILEYTDPYKALQEKVRVYNNILLFNNEKIPFKKPILISIGKASLPMARFFRERMELKAKLIVTPKGTNGKENDVIEAGHPLPDENSIKAGKRMIELLANEDYDLVIFAISGGASALVEYSEIPLDELKIINKVLVTSGLGINKINIVRKHLSKVKGGKILEYVKDKIPIVSFIVSDVPGNDISSIGSGLTSIDNSSNDDALEILKAIGLEKYSKYLTETPKSFSRIVKNYIILDNMEVLRKLANTLVNSFILTSEIRGEARDVGAIIASIYNSSESYNIPFRRPYYLLVGGEPEVTIQGKAGKGGRNGEVCLSFLKYAKKRNRFELLGFATDGIDGNSEYAGCKVSSDMEIREDEINNALETHNSYGLLESHKAVIKTGYTHTNVNNIYVLRAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXQ3</accession>
    <name>Q7LXQ3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF309 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0652</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90212">
        <property type="entry name" value="F90212"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991190.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXQ3"/>
    <dbReference type="PaxDb" id="273057-SSO0652"/>
    <dbReference type="EnsemblBacteria" id="AAK40957">
        <property type="protein sequence ID" value="AAK40957"/>
        <property type="gene ID" value="SSO0652"/>
    </dbReference>
    <dbReference type="GeneID" id="34442647"/>
    <dbReference type="KEGG" id="sso:SSO0652"/>
    <dbReference type="eggNOG" id="arCOG03705">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2021601_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXQ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.10">
        <property type="entry name" value="TTHA0068-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005500">
        <property type="entry name" value="DUF309"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023203">
        <property type="entry name" value="TTHA0068_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03745">
        <property type="entry name" value="DUF309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140663">
        <property type="entry name" value="TTHA0068-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="17562" checksum="C6410FFDC8F25BBB" modified="2011-05-31" version="1">MLGRVLLFYKKGIFNDDLKKFLRSKNINVIDVRIGKYVEVDIMDDPRKVISLIGEPLFMVTEISGTFKPLFYEMRFWECHEIIEEKWREVKNKDDKKFLQAIILLCASLIKYLKGEIEVSDMLMEKALSLISDLPQELLPLLYISLGLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXR8</accession>
    <name>Q7LXR8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90209">
        <property type="entry name" value="D90209"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991154.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXR8"/>
    <dbReference type="STRING" id="273057.SSO0620"/>
    <dbReference type="PaxDb" id="273057-SSO0620"/>
    <dbReference type="EnsemblBacteria" id="AAK40931">
        <property type="protein sequence ID" value="AAK40931"/>
        <property type="gene ID" value="SSO0620"/>
    </dbReference>
    <dbReference type="GeneID" id="1454898"/>
    <dbReference type="KEGG" id="sso:SSO0620"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.628"/>
    <dbReference type="eggNOG" id="arCOG00731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153620_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LXR8"/>
    <dbReference type="PhylomeDB" id="Q7LXR8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38600">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38600:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="105" mass="12604" checksum="FDBD1C47400F2FF3" modified="2011-05-31" version="1">MNEKERKQVKKFFRFLFFTSRGGITRLKIVRVLEEKPLNPNQIASTLKLDYKTVMHHLEVLMENNIVYREADGYGLPYKLTTFYKTYKDVLDELEREIRQKANKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXT2</accession>
    <name>Q7LXT2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">SSO0586</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90205">
        <property type="entry name" value="E90205"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991105.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXT2"/>
    <dbReference type="FunCoup" id="Q7LXT2">
        <property type="interactions" value="149"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0586"/>
    <dbReference type="PaxDb" id="273057-SSO0586"/>
    <dbReference type="EnsemblBacteria" id="AAK40900">
        <property type="protein sequence ID" value="AAK40900"/>
        <property type="gene ID" value="SSO0586"/>
    </dbReference>
    <dbReference type="GeneID" id="1454867"/>
    <dbReference type="KEGG" id="sso:SSO0586"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.596"/>
    <dbReference type="eggNOG" id="arCOG00042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026481_1_1_2"/>
    <dbReference type="InParanoid" id="Q7LXT2"/>
    <dbReference type="PhylomeDB" id="Q7LXT2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="CTU1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000174">
        <property type="entry name" value="ATPase, PP-loop superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056369">
        <property type="entry name" value="CTU1-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054306">
        <property type="entry name" value="TtuA-like_LIM_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00269">
        <property type="entry name" value="TIGR00269 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22082">
        <property type="entry name" value="TtuA_LIM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="2-thiouridine synthetase TtuA-like N-terminal LIM" evidence="15">
        <location>
            <begin position="3"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="14">
        <location>
            <begin position="49"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="3"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF22082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AAK40900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="323" mass="37166" checksum="7F860AC79D93AC56" modified="2011-05-31" version="1">MICDNCKTREAVILQPHTGRKLCKECFIEDIRKRVEMEARKQEIVNSNKILLAVSGGKDSLVLADTLSQFIHPSRLIAFNINEGIKGYNRNEYVKKLEEYLKDLGIELIKSGFKEEVGFSLDEMLEASLKKKLNVSACTFCGGFRRKLINEAGIKVAADYVATGHNLDDEVQTIVINLIRGDLLRLIRFGDKPLMVSSKFVMRVKPLRKIYEWETTMYAHLKGFEFQETECPYISQKPTLRAKVRDLLYKLEEKKPGTLLRILEQFDEISEKIKKEYRLTNELPNCVICGEPTTPGRMICKNCELLIRSGLMPQEYQKYLPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TV6</accession>
    <name>Q97TV6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1367</name>
        <name evidence="3" type="ordered locus">SSO2086</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90293">
        <property type="entry name" value="B90293"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923358.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TV6"/>
    <dbReference type="PaxDb" id="273057-SSO1367"/>
    <dbReference type="EnsemblBacteria" id="AAK41601">
        <property type="protein sequence ID" value="AAK41601"/>
        <property type="gene ID" value="SSO1367"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK42265">
        <property type="protein sequence ID" value="AAK42265"/>
        <property type="gene ID" value="SSO2086"/>
    </dbReference>
    <dbReference type="GeneID" id="1454380"/>
    <dbReference type="KEGG" id="sso:SSO1367"/>
    <dbReference type="KEGG" id="sso:SSO2086"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1372"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="80"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37370" checksum="7F9A3029E06797AE" modified="2001-10-01" version="1">MQTIQVSKTELKSLAITLATNNINVISQDLDPEIVKAAPSLLTGNRGKYYLKVVRRGEKVISKGQRTFKFYPIYREVKGEINVVAVDETGLTVGEKEQEKAEGFLLYNWKRKGVKMRSLDLVYPLRLPLLVEVADLRSDSPSQFLLRSVREVSQYMEIDYVVADAGFLNLGVIKEMPVKTIVRGKSNLKGFKELSNVPLVEKRYEVKDKVYVAYRVLKFEGLYYYDVVYVKGKPRHFMFVTNFEGDPYELAELYRLRWQVEEGFKVRKARIRYVRKLSNKIFLFLYYTVLDSAWNLVNHLLFNFKSTCKKVLSFDSFVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TY1</accession>
    <name>Q97TY1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO12252</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90510">
        <property type="entry name" value="H90510"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989654.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TY1"/>
    <dbReference type="PaxDb" id="273057-SSO12252"/>
    <dbReference type="EnsemblBacteria" id="AAK43345">
        <property type="protein sequence ID" value="AAK43345"/>
        <property type="gene ID" value="SSO12252"/>
    </dbReference>
    <dbReference type="GeneID" id="1453262"/>
    <dbReference type="KEGG" id="sso:SSO12252"/>
    <dbReference type="eggNOG" id="arCOG07177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173009_0_0_2"/>
    <dbReference type="InParanoid" id="Q97TY1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="83" mass="9654" checksum="AFD28ED59BE0E28C" modified="2001-10-01" version="1">MFKELLGDYEPRGRIIQGVGRFIALGLDDPFHITTITGFTLYGIGKYIEKKRTNYGIRMAKMDIENIRKNLREITLDISTIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TZ1</accession>
    <name>Q97TZ1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PUA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90509">
        <property type="entry name" value="F90509"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989670.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TZ1"/>
    <dbReference type="FunCoup" id="Q97TZ1">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3240"/>
    <dbReference type="PaxDb" id="273057-SSO3240"/>
    <dbReference type="EnsemblBacteria" id="AAK43333">
        <property type="protein sequence ID" value="AAK43333"/>
        <property type="gene ID" value="SSO3240"/>
    </dbReference>
    <dbReference type="GeneID" id="1453252"/>
    <dbReference type="KEGG" id="sso:SSO3240"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3340"/>
    <dbReference type="eggNOG" id="arCOG00985">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090468_1_1_2"/>
    <dbReference type="InParanoid" id="Q97TZ1"/>
    <dbReference type="PhylomeDB" id="Q97TZ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21154">
        <property type="entry name" value="PUA_MJ1432-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.120:FF:000001">
        <property type="entry name" value="DUF1947 domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.10:FF:000017">
        <property type="entry name" value="RNA-binding protein, containing PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.120">
        <property type="entry name" value="Pre-PUA domain, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022430">
        <property type="entry name" value="CHP03684"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015266">
        <property type="entry name" value="DUF1947"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03684">
        <property type="entry name" value="arCOG00985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011150">
        <property type="entry name" value="PRK14560.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798:SF0">
        <property type="entry name" value="MALIGNANT T-CELL-AMPLIFIED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="MCT-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09183">
        <property type="entry name" value="DUF1947"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005067">
        <property type="entry name" value="Tma_RNA-bind_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="69"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="152" mass="17279" checksum="46B0205EDC6B1701" modified="2001-10-01" version="1">MQRHVMSSKDSKIFINKIKEKYNVDISNAKLEVGKEKKEIWYYVNDILAFFDDLIPTLCAIIRLKINLPYVIIDEGAVKAVSKGADLFAPGIVEYNCECKENEIVLAKTKTNLPVAVLRVLMPKEKALVEKKGKFALNLHHIGDKIWEMCNG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-03-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TZ8</accession>
    <name>GUAAB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>GMP synthase [glutamine-hydrolyzing] subunit B</fullName>
            <ecNumber>6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>GMP synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">guaAB</name>
        <name type="synonym">guaA</name>
        <name type="ordered locus">SSO3232</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>XMP + L-glutamine + ATP + H2O = GMP + L-glutamate + AMP + diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90508">
        <property type="entry name" value="G90508"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989678.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TZ8"/>
    <dbReference type="SMR" id="Q97TZ8"/>
    <dbReference type="FunCoup" id="Q97TZ8">
        <property type="interactions" value="361"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3232"/>
    <dbReference type="PaxDb" id="273057-SSO3232"/>
    <dbReference type="EnsemblBacteria" id="AAK43326">
        <property type="protein sequence ID" value="AAK43326"/>
        <property type="gene ID" value="SSO3232"/>
    </dbReference>
    <dbReference type="GeneID" id="1453245"/>
    <dbReference type="KEGG" id="sso:SSO3232"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3334"/>
    <dbReference type="eggNOG" id="arCOG00085">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_0_0_2"/>
    <dbReference type="InParanoid" id="Q97TZ8"/>
    <dbReference type="PhylomeDB" id="Q97TZ8"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00345">
        <property type="entry name" value="GMP_synthase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026598">
        <property type="entry name" value="GMP_synthase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54810">
        <property type="entry name" value="GMP synthetase C-terminal dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0332">GMP biosynthesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140251" description="GMP synthase [glutamine-hydrolyzing] subunit B">
        <location>
            <begin position="1"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="GMPS ATP-PPase">
        <location>
            <begin position="2"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="367" mass="41383" checksum="02D30198868AC698" modified="2001-10-01" version="1">MFDPASFVEEIGPQLRQKLGNEKVLAAVSGGVDSTTAAVLAYNLLGDKVIPVLVDTGFLRKNEAEKIKDYLSNILPNLIIVDKKETFISEIEGIDDAEMKRKRFRELFYSTISSLMKKFNARYLMQGTIAADWVETQGGIKTQHNVLVQIGIDTEKEWGFTLIEPLADLYKNEVRELARYLKLPKEISERQPFPGPGLLVRAVGKLTREKLEVVREANDIVEKYLDPFNYSQYFAVSFESYGNLVNLDGIDAFLYKARATGVKGDVRAYGNIAKIECSNINNVKNIIDTLVKYDITHVLCTLDERNSGKYSVAIRAVTTEDFMTADYVRIPKEILSKISSEILQKIPNVKEVLYDVTSKPPATIEFE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-01-31" modified="2025-06-18" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UA2</accession>
    <name>PIP_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Proline iminopeptidase</fullName>
            <shortName>PIP</shortName>
            <ecNumber>3.4.11.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Prolyl aminopeptidase</fullName>
            <shortName>PAP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Tricorn protease-interacting factor F1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pip</name>
        <name type="ordered locus">SSO3115</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cleaves H-Pro-AMC as well as a wide spectrum of amino acid substrates and several peptide substrates without a proline at the N-terminus. In conjunction with the three factors F1, F2 and F3, Tricorn degrades oligopeptides in a sequential manner, yielding free amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Release of N-terminal proline from a peptide.</text>
            <dbReference type="EC" id="3.4.11.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the tricorn proteolytic complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90495">
        <property type="entry name" value="D90495"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UA2"/>
    <dbReference type="SMR" id="Q97UA2"/>
    <dbReference type="FunCoup" id="Q97UA2">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3115"/>
    <dbReference type="ESTHER" id="sulso-pip">
        <property type="family name" value="Proline_iminopeptidase"/>
    </dbReference>
    <dbReference type="MEROPS" id="S33.005"/>
    <dbReference type="PaxDb" id="273057-SSO3115"/>
    <dbReference type="EnsemblBacteria" id="AAK43219">
        <property type="protein sequence ID" value="AAK43219"/>
        <property type="gene ID" value="SSO3115"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3115"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3224"/>
    <dbReference type="eggNOG" id="arCOG01648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_15_1_2"/>
    <dbReference type="InParanoid" id="Q97UA2"/>
    <dbReference type="PhylomeDB" id="Q97UA2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002410">
        <property type="entry name" value="Peptidase_S33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005945">
        <property type="entry name" value="Pro_imino_pep"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01250">
        <property type="entry name" value="pro_imino_pep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045948">
        <property type="entry name" value="ProImpepThrmp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF31">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN YCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005539">
        <property type="entry name" value="Pept_S33_TRI_F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00793">
        <property type="entry name" value="PROAMNOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0031">Aminopeptidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000080850" description="Proline iminopeptidase">
        <location>
            <begin position="1"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="41"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="310" mass="35961" checksum="B50DE89D0A323EC3" modified="2001-10-01" version="1">MSYYFITMKGYSINVEEGYKRIFGINIYYKLYRVKGSNRNLVTLHGGPGGSHDYLIPLADLSNYGINVLFYDQFGCGRSDDPKDTSDYTIDHGLEELEELRKQVFGNDKIVLLGHSYGGALAIAYALKYQQFLRGLIVSSGLSSVPYTVKEMRRLIEELPDKYKTIIKRYESLGDFKNPEYLDAVNFFYSQHLLRLKEMPEPVKRTFEYIGKRRTYEIMNGPNEFTIIGTIKDWDVTEQLYKITVPTLITVGKYDEVTVNVAQLIHKNIKGSRLVIFENSSHMAMWEEKDKYLEVIKEFIDQVYSLNMVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UK0</accession>
    <name>Q97UK0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dehydrogenase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO3011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90482">
        <property type="entry name" value="B90482"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992665.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UK0"/>
    <dbReference type="FunCoup" id="Q97UK0">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3011"/>
    <dbReference type="PaxDb" id="273057-SSO3011"/>
    <dbReference type="DNASU" id="1453032"/>
    <dbReference type="EnsemblBacteria" id="AAK43113">
        <property type="protein sequence ID" value="AAK43113"/>
        <property type="gene ID" value="SSO3011"/>
    </dbReference>
    <dbReference type="GeneID" id="1453032"/>
    <dbReference type="KEGG" id="sso:SSO3011"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3103"/>
    <dbReference type="eggNOG" id="arCOG01622">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_1_4_2"/>
    <dbReference type="InParanoid" id="Q97UK0"/>
    <dbReference type="PhylomeDB" id="Q97UK0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051450">
        <property type="entry name" value="Gfo/Idh/MocA_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377:SF1">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="2">
        <location>
            <begin position="133"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="363" mass="40410" checksum="9C83D033D85D1E81" modified="2001-10-01" version="1">MLAMKLGIVGLGGWVTGAHIPALTDLGIKVDYCADIDEKRVKDLSQKTGCKGYLDYKEMLRNENPDLVLVAVPHSLHAIIALDAINNDANVYVEKPMATSFQEALTLVEASRKRNKILVVGHENRFNPAMLIAKKLIRGGEVGKIYHMRGFYIRQRGIPTSPTFIKKDLAKGGAVFDIGTHIIDLLLFLSDFPTPKSVTGKTYNAFSEDKTKFSAYPSPNLPNFKVEVEDFGSAFLKLGDEISAYVEVSWASYIKENKMEVVILGDNGGIHIDNGVLNFMRNIEDELFISSPLTQQKGSVYREVWRTVMESISKGFPEYPLCSAEQGAIGVAILESIYKSALEGREVKIDIPQWFLKQFQPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UM9</accession>
    <name>Q97UM9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1404 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90477">
        <property type="entry name" value="H90477"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924082.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UM9"/>
    <dbReference type="STRING" id="273057.SSO2975"/>
    <dbReference type="PaxDb" id="273057-SSO2975"/>
    <dbReference type="EnsemblBacteria" id="AAK43079">
        <property type="protein sequence ID" value="AAK43079"/>
        <property type="gene ID" value="SSO2975"/>
    </dbReference>
    <dbReference type="GeneID" id="1452998"/>
    <dbReference type="KEGG" id="sso:SSO2975"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3065"/>
    <dbReference type="eggNOG" id="arCOG06032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109678_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UM9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009844">
        <property type="entry name" value="DUF1404"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07185">
        <property type="entry name" value="DUF1404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="23133" checksum="C6F18F3F025D4166" modified="2001-10-01" version="1">MNNYFFITILMFCIFLIAISVNPFTEEYMFVNPIPYMLAHYSLFGAGILISYYLVKRKIVDKHIGFPVGASIAFLWHYPFFFNLGAEILSVRLIEEITLLVGGLIIGSSLKELNRSFKVLLLALWMIGDSLLSLILMISPSLYTTLYAPKELEYLGIIMFLMMNLIAVYILLNYINNLLRDEENVVNKDYERNSYPKNDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UT6</accession>
    <name>Q97UT6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phytoene dehydrogenase (Desaturase) (CrtI)</fullName>
            <ecNumber evidence="5">1.3.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">crtI</name>
        <name evidence="5" type="ordered locus">SSO2907</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carotenoid biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="1.3.-.-" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99470">
        <property type="entry name" value="A99470"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UT6"/>
    <dbReference type="FunCoup" id="Q97UT6">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2907"/>
    <dbReference type="PaxDb" id="273057-SSO2907"/>
    <dbReference type="EnsemblBacteria" id="AAK43016">
        <property type="protein sequence ID" value="AAK43016"/>
        <property type="gene ID" value="SSO2907"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2907"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2997"/>
    <dbReference type="eggNOG" id="arCOG01521">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019722_2_1_2"/>
    <dbReference type="InParanoid" id="Q97UT6"/>
    <dbReference type="PhylomeDB" id="Q97UT6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014105">
        <property type="entry name" value="Carotenoid/retinoid_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02734">
        <property type="entry name" value="crtI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734:SF1">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="4">
        <location>
            <begin position="11"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="454" mass="52011" checksum="9F40C18D51C0272B" modified="2001-10-01" version="1">MMKAVVVGAGLGGISTAILLKKFFDEVVVLEKNTTPGGRARYFSVGEFRFDMGPSWYLMPEIFEEFFSEAGENPHPIIRVNPPVKVMESKFLKKEYREFTGDNVDDRYLNKTRLFYDFSKKFIKKEMVFSDFLDKEVLVNISALFDTLDNFNARYFRDSLMRKAMGFSSVFIGGSPFRVPAVYAMINYAIYGQGVFYPYKGFEGYVKRLYEIARKKGVEFKFDFPVNKVKIDEGKVVEVSSNIKREMGDVFVFNMDYFYADSLLPEEYKVISGRRYKLSPSAILAYIGLEDTLDLPHHTVIINGDWREHFTSIDRNSLPSVENTSYYVSYRGATNKETRDLVILIPVGTGTEISKELIDGVIRDLEVKIGCKIKASYIRIYGPDDFKRDYNAYEGTAFGLAHTLDQTGPFRLPMKNTRLKNLYYVGQYTQPGIGVPMVTLSSMIVSKKISKEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UU1</accession>
    <name>Q97UU1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2902</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90469">
        <property type="entry name" value="D90469"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924026.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UU1"/>
    <dbReference type="STRING" id="273057.SSO2902"/>
    <dbReference type="PaxDb" id="273057-SSO2902"/>
    <dbReference type="EnsemblBacteria" id="AAK43011">
        <property type="protein sequence ID" value="AAK43011"/>
        <property type="gene ID" value="SSO2902"/>
    </dbReference>
    <dbReference type="GeneID" id="1452930"/>
    <dbReference type="KEGG" id="sso:SSO2902"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2992"/>
    <dbReference type="eggNOG" id="arCOG07726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2127954_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UU1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="113" mass="12871" checksum="4FFDCB1A31C28048" modified="2001-10-01" version="1">MLQKLEVEIITMIKSMVGFMLNFAGEVVGEFVNSGSFAIVRPKEKVMDETFINSHEDLLLSITSKYYISNIKVKEMRGASLGFGKLRFAKTFVNEIYFNYLIRTGILELKSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="150" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UU7</accession>
    <name>Q97UU7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Copper-transporting ATPase</fullName>
            <ecNumber evidence="15">3.6.3.10</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">SSO2896</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2007" name="J. Mol. Biol." volume="369" first="368" last="385">
            <title>Sulfate acts as phosphate analog on the monomeric catalytic fragment of the CPx-ATPase CopB from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Lubben M."/>
                <person name="Guldenhaupt J."/>
                <person name="Zoltner M."/>
                <person name="Deigweiher K."/>
                <person name="Haebel P."/>
                <person name="Urbanke C."/>
                <person name="Scheidig A.J."/>
            </authorList>
            <dbReference type="PubMed" id="17434529"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2007.03.029"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 383-645</scope>
    </reference>
    <reference evidence="18 19 20" key="3">
        <citation type="submission" date="2011-05" db="PDB data bank">
            <title>Conformational Changes in the Catalytic Domain of the Cpx-ATPase Copb-B Upon Nucleotide Binding.</title>
            <authorList>
                <person name="Voellmecke C."/>
                <person name="Schlicker C."/>
                <person name="Luebben M."/>
                <person name="Hofmann E."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 383-645 IN COMPLEX WITH AMP; ATP AND MG(2+)</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.10" evidence="15"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90468">
        <property type="entry name" value="D90468"/>
    </dbReference>
    <dbReference type="PDB" id="2IYE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A/C=383-645"/>
    </dbReference>
    <dbReference type="PDB" id="2YJ3">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A=383-645"/>
    </dbReference>
    <dbReference type="PDB" id="2YJ4">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=383-645"/>
    </dbReference>
    <dbReference type="PDB" id="2YJ5">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=383-645"/>
    </dbReference>
    <dbReference type="PDB" id="2YJ6">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B=383-645"/>
    </dbReference>
    <dbReference type="PDBsum" id="2IYE"/>
    <dbReference type="PDBsum" id="2YJ3"/>
    <dbReference type="PDBsum" id="2YJ4"/>
    <dbReference type="PDBsum" id="2YJ5"/>
    <dbReference type="PDBsum" id="2YJ6"/>
    <dbReference type="AlphaFoldDB" id="Q97UU7"/>
    <dbReference type="SMR" id="Q97UU7"/>
    <dbReference type="STRING" id="273057.SSO2896"/>
    <dbReference type="TCDB" id="3.A.3.5.13">
        <property type="family name" value="the p-type atpase (p-atpase) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2896"/>
    <dbReference type="EnsemblBacteria" id="AAK43003">
        <property type="protein sequence ID" value="AAK43003"/>
        <property type="gene ID" value="SSO2896"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2896"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2983"/>
    <dbReference type="eggNOG" id="arCOG01576">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_11_2_2"/>
    <dbReference type="InParanoid" id="Q97UU7"/>
    <dbReference type="PhylomeDB" id="Q97UU7"/>
    <dbReference type="BRENDA" id="7.2.2.9">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97UU7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000001">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000020">
        <property type="entry name" value="Probable cation-transporting P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051014">
        <property type="entry name" value="Cation_Transport_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01512">
        <property type="entry name" value="ATPase-IB2_Cd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48085">
        <property type="entry name" value="CADMIUM/ZINC-TRANSPORTING ATPASE HMA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48085:SF1">
        <property type="entry name" value="CATION TRANSPORTING ATPASE (E1-E2 FAMILY)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00943">
        <property type="entry name" value="CUATPASE"/>
    </dbReference>
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        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
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        <property type="entry name" value="HMA, heavy metal-associated domain"/>
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    </dbReference>
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        <property type="entry name" value="ATPASE_E1_E2"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="17 18 19" id="KW-0002">3D-structure</keyword>
    <keyword evidence="8 19 20" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="7 19 20" id="KW-0547">Nucleotide-binding</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="116"/>
            <end position="137"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="149"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="329"/>
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            <begin position="357"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="642"/>
            <end position="663"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="669"/>
            <end position="689"/>
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        <location>
            <begin position="8"/>
            <end position="74"/>
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            <position position="417"/>
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            <label>1</label>
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            <name>Mg(2+)</name>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS50846"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAK43003.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
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    </evidence>
    <evidence type="ECO:0007829" key="17">
        <source>
            <dbReference type="PDB" id="2IYE"/>
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    </evidence>
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        <source>
            <dbReference type="PDB" id="2YJ3"/>
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        <source>
            <dbReference type="PDB" id="2YJ5"/>
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    <sequence length="695" mass="76330" checksum="5BE56F618D3B3840" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UV1</accession>
    <name>SDX_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Probable sulredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">sdx</name>
        <name type="ordered locus">SSO2891</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="2">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SDX family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK42999" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42999.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="H90467"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    <dbReference type="SMR" id="Q97UV1"/>
    <dbReference type="STRING" id="273057.SSO2891"/>
    <dbReference type="PaxDb" id="273057-SSO2891"/>
    <dbReference type="EnsemblBacteria" id="AAK42999">
        <property type="protein sequence ID" value="AAK42999"/>
        <property type="gene ID" value="SSO2891"/>
    </dbReference>
    <dbReference type="GeneID" id="1452918"/>
    <dbReference type="GeneID" id="44128619"/>
    <dbReference type="KEGG" id="sso:SSO2891"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2979"/>
    <dbReference type="eggNOG" id="arCOG02854">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_5_4_2"/>
    <dbReference type="InParanoid" id="Q97UV1"/>
    <dbReference type="PhylomeDB" id="Q97UV1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03467">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.102.10.10:FF:000038">
        <property type="entry name" value="Rieske (2Fe-2S) protein"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
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    <dbReference type="InterPro" id="IPR053457">
        <property type="entry name" value="SDX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041174">
        <property type="entry name" value="SDX_Thmprot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496">
        <property type="entry name" value="FERREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496:SF24">
        <property type="entry name" value="SULREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000127795" description="Probable sulredoxin">
        <location>
            <begin position="2"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Rieske" evidence="2">
        <location>
            <begin position="3"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
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        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="109" mass="12227" checksum="5A3189535B8241E0" modified="2007-01-23" version="2">MVWKRTISAKALEKAKSAAVKVEDKVVFIANIKGTLYAMDAVCSHARCILDQLDEEKLTVKCYCHQALFDLRSGKMLEPPYVAPDAPKEKLGLKTYQIRDNGGWIEVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UW0</accession>
    <name>Q97UW0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4-carboxymucolactone decarboxylase (PcaC)</fullName>
            <ecNumber evidence="2">4.1.1.44</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pcaC</name>
        <name evidence="2" type="ordered locus">SSO2884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
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        <property type="entry name" value="G90466"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="SSO2884"/>
    </dbReference>
    <dbReference type="GeneID" id="1452909"/>
    <dbReference type="KEGG" id="sso:SSO2884"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2970"/>
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    <evidence type="ECO:0000259" key="1">
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAK42990.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="139" mass="15945" checksum="367760624C7703AD" modified="2001-10-01" version="1">MGSLFDKDPELKKLYEKGLEVRKKVMGREYVEKSLSQTTDFNRELQEMVTIFAWGLIWTRDDVIPKKIRSLINIGILSCLNRQNELKLHVKGAIRNGCTEEEIKEVLLQVGAYCGLPAAMDSFRIAQEAIKELKEEEKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-01-24" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UY4</accession>
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        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1439</fullName>
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        <name evidence="2" type="ordered locus">SSO2855</name>
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        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="E90463"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UY4"/>
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    <dbReference type="EnsemblBacteria" id="AAK42964">
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        <property type="gene ID" value="SSO2855"/>
    </dbReference>
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    <dbReference type="eggNOG" id="arCOG03902">
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    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RNaseH-like_sf"/>
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        <property type="entry name" value="DDE_Tnp_1"/>
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    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="80"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="291" mass="33525" checksum="7F66785834058208" modified="2001-10-01" version="1">MQVYETGFKTELKSLASYFIVNNLNVISQELDTRIADAAPFLLTGNRGKEYLKVVREGEKVMSQGERSFKFYPHVKVEGETTIVAVDETAITVGEKESRKVEGFQLYNGKRKGVKLRSVDLVFPLRLSLLEEIADLRSDTPSQFLMRAVSEVAQHLKIDYVVADAGFLNLKVIKEMPVKTVVGGKTNLKGFKELSNVSLTEKKCEVNDRVYVAYRVLKYNDLYYYEVIYVKEKPRHFIFVTNFNGDPYKLAELYRLRWQSEEGFKVRKARIRYVSAFRKFIALGKGYTTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V07</accession>
    <name>Q97V07_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="E90460"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V07"/>
    <dbReference type="STRING" id="273057.SSO2830"/>
    <dbReference type="PaxDb" id="273057-SSO2830"/>
    <dbReference type="EnsemblBacteria" id="AAK42940">
        <property type="protein sequence ID" value="AAK42940"/>
        <property type="gene ID" value="SSO2830"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2830"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2915"/>
    <dbReference type="eggNOG" id="arCOG08510">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_502166_0_0_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="294"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="516"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="545" mass="62571" checksum="0D6FF1E408C5BD81" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V20</accession>
    <name>Q97V20_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="6">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">SSO2816</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90458">
        <property type="entry name" value="H90458"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V20"/>
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        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2816"/>
    <dbReference type="PaxDb" id="273057-SSO2816"/>
    <dbReference type="EnsemblBacteria" id="AAK42927">
        <property type="protein sequence ID" value="AAK42927"/>
        <property type="gene ID" value="SSO2816"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2816"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2902"/>
    <dbReference type="eggNOG" id="arCOG01599">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_048564_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V20"/>
    <dbReference type="PhylomeDB" id="Q97V20"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
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    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:thiamine pyrophosphate binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="TPP_OGFOR"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="2-oxoglutarate ferredoxin oxidoreductase subunit beta"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053399">
        <property type="entry name" value="2-oxoacid:Fd_oxidored_beta"/>
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    <dbReference type="InterPro" id="IPR051457">
        <property type="entry name" value="2-oxoacid:Fd_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011896">
        <property type="entry name" value="OFOB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032686">
        <property type="entry name" value="PFO_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041171">
        <property type="entry name" value="Oxoac_fdxbeta_Archa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02177">
        <property type="entry name" value="PorB_KorB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084">
        <property type="entry name" value="2-OXOGLUTARATE OXIDOREDUCTASE SUBUNIT KORB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084:SF2">
        <property type="entry name" value="PYRUVATE FERREDOXIN_FLAVODOXIN OXIDOREDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12367">
        <property type="entry name" value="PFO_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="14">
        <location>
            <begin position="56"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate ferredoxin oxidoreductase beta subunit C-terminal" evidence="15">
        <location>
            <begin position="208"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AAK42927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="316" mass="35030" checksum="4196F19F39585500" modified="2001-10-01" version="1">MFKKEKGGWFYMAGLKVEWNDWCPGCGNFGILSAEQQAIQELGLDPKKVVLVSGIGCSGKIPHFIRLPASGVHTLHGRALTFAIGIKLANPSLEVIVNGGDGDQLGIGVGHFVSAGRRNVDLTVIVHNNGVYGLTKGQASPTLKLGVKTKSLPKPNINSDINPIALAISSGYTFVARGYAYDVKHLKEIIKKAIKHKGLAMIDVLQPCPTYNDIHTKEYYDKRVYKLDEDPSWDPIVKKPEEMDDKMSKAILKSMEWGDRIPIGIFYQNELVSTYEQRIAERSPSYLDNPPAHDVIEFEGKPTTDVEDILKERRVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V63</accession>
    <name>Q97V63_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (HpcE-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hpcE-2</name>
        <name evidence="4" type="ordered locus">SSO2773</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="C90453"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="AAK42882">
        <property type="protein sequence ID" value="AAK42882"/>
        <property type="gene ID" value="SSO2773"/>
    </dbReference>
    <dbReference type="GeneID" id="1452801"/>
    <dbReference type="KEGG" id="sso:SSO2773"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2854"/>
    <dbReference type="eggNOG" id="arCOG00235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_3_1_2"/>
    <dbReference type="InParanoid" id="Q97V63"/>
    <dbReference type="PhylomeDB" id="Q97V63"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="2-hydroxyhepta-2,4-diene-1,7-dioate isomerase"/>
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    </dbReference>
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        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAH"/>
    </dbReference>
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        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
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        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="93"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="304" mass="34593" checksum="36DC3F667AD2ED8F" modified="2001-10-01" version="1">MVKILLFSPNPNEFKRVGVYENGRVIDLVKAYELLYDAKPPNWFYDTKDLIEGGNGSILLIKSVLDSLRGKEDKVSYDPEKIFYYPPITNPEKIFLLAVNYKAHGKETNNEPPKEPYIFTKFSNALIGHNQPILYPKASNKVDYEIELGVIIGKQGKYVKRERAFDYVFGYTVFNDISYRDRQFPSEMPYGMRWVHGKGMDTGAPMGPWIVTKDEIPNPYELRLTLRVNGEVRQDAYAEDMIFKIDEIIEYLSNGITLKPGDIISTGTPPGVGLATGKFLKPGDTIEAEISKIGVLRNYLVKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V88</accession>
    <name>Q97V88_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aldose 1-epimerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2747</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90450">
        <property type="entry name" value="B90450"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990266.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V88"/>
    <dbReference type="FunCoup" id="Q97V88">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2747"/>
    <dbReference type="PaxDb" id="273057-SSO2747"/>
    <dbReference type="DNASU" id="1452776"/>
    <dbReference type="EnsemblBacteria" id="AAK42857">
        <property type="protein sequence ID" value="AAK42857"/>
        <property type="gene ID" value="SSO2747"/>
    </dbReference>
    <dbReference type="GeneID" id="1452776"/>
    <dbReference type="KEGG" id="sso:SSO2747"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2826"/>
    <dbReference type="eggNOG" id="arCOG05361">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052486_1_1_2"/>
    <dbReference type="InParanoid" id="Q97V88"/>
    <dbReference type="PhylomeDB" id="Q97V88"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01081">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091">
        <property type="entry name" value="ALDOSE-1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091:SF0">
        <property type="entry name" value="GALACTOSE MUTAROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="29710" checksum="B9827B6F0476383D" modified="2001-10-01" version="1">MKIKKGDTEAEILTKGAYLYSFKIGNRDMILRGDLERPTRGGMAILIPFANRVKNAEYVFEGVKYTLPQNREGNAIHGLVMDKEFNVIGKSEDSISLQYILEHEGYPSKLDCVITYKVFKYGLRIKIVVRNVGNKRAPLTVGAHPYFITADDWRIVVEKEESIKKCISFNKIPTGELVKTKLEHTDYDDCFLISGGIQLHSSYSKVRITRRNMPFVQIYTGVRGSIAIEPMSGAPDAYHNGLGLKILEPNESSYFAFAFRFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VB0</accession>
    <name>Q97VB0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial transposase ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO10982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90447">
        <property type="entry name" value="C90447"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VB0"/>
    <dbReference type="STRING" id="273057.SSO10982"/>
    <dbReference type="PaxDb" id="273057-SSO10982"/>
    <dbReference type="EnsemblBacteria" id="AAK42834">
        <property type="protein sequence ID" value="AAK42834"/>
        <property type="gene ID" value="SSO10982"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO10982"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192666_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VB0"/>
    <dbReference type="PhylomeDB" id="Q97VB0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="84" mass="10086" checksum="715604841DF18A15" modified="2001-10-01" version="1">MMYFARATNLDLTKREVLELYNKVRGPIETSYKNIKTFLPFTSSTKFVFRELIFVLAMVFYSLYTVFKDVMRREEFRLLSSSVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VC9</accession>
    <name>Q97VC9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2703</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90444">
        <property type="entry name" value="H90444"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988630.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VC9"/>
    <dbReference type="STRING" id="273057.SSO2703"/>
    <dbReference type="PaxDb" id="273057-SSO2703"/>
    <dbReference type="EnsemblBacteria" id="AAK42815">
        <property type="protein sequence ID" value="AAK42815"/>
        <property type="gene ID" value="SSO2703"/>
    </dbReference>
    <dbReference type="GeneID" id="1452734"/>
    <dbReference type="KEGG" id="sso:SSO2703"/>
    <dbReference type="eggNOG" id="arCOG03868">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161777_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VC9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="131" mass="14818" checksum="78D0B2BB46CCA340" modified="2001-10-01" version="1">MPRVSISLLALGVVAGIASFAWTADHLPLYSIRFLPYSIRAFFLFDSILAIIAGILFILSFRLFTLKIIYLLEVIYWWINYLLLTLTRVFPAPLIGRPLPVTTGPALIAFVLDIILIILSTTIYIFLTSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VG5</accession>
    <name>Q97VG5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2661</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90440">
        <property type="entry name" value="D90440"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VG5"/>
    <dbReference type="STRING" id="273057.SSO2661"/>
    <dbReference type="PaxDb" id="273057-SSO2661"/>
    <dbReference type="EnsemblBacteria" id="AAK42779">
        <property type="protein sequence ID" value="AAK42779"/>
        <property type="gene ID" value="SSO2661"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2661"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2743"/>
    <dbReference type="eggNOG" id="arCOG04030">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1709219_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VG5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="186" mass="20392" checksum="9ED3F38BF63BFC2D" modified="2001-10-01" version="1">MRIYLHMMNQLAVQKCYYNLVKHLVIATLSLRALETPTNEVTSLGAIEMMYEKYIQMSKMMSRMNYMPAIAAGEITILLILYAGGMLLAVYNLSLQGVPLIMHLYGAILVAILSVGLLASAVSYKDKGAIMLSFLNVLSVLLAAFAGSFYFGGVIDVSYLLEMGMGFVFALITASGCLVYSIRRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VH5</accession>
    <name>Q97VH5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2648</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90439">
        <property type="entry name" value="B90439"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988558.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VH5"/>
    <dbReference type="STRING" id="273057.SSO2648"/>
    <dbReference type="PaxDb" id="273057-SSO2648"/>
    <dbReference type="EnsemblBacteria" id="AAK42769">
        <property type="protein sequence ID" value="AAK42769"/>
        <property type="gene ID" value="SSO2648"/>
    </dbReference>
    <dbReference type="GeneID" id="1452688"/>
    <dbReference type="KEGG" id="sso:SSO2648"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2731"/>
    <dbReference type="eggNOG" id="arCOG05996">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727316_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VH5"/>
    <dbReference type="PhylomeDB" id="Q97VH5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="145" mass="16385" checksum="DA0E24C1C8D421B9" modified="2001-10-01" version="1">MTNESIKYIAIKMLADKAYVVDAIYSYLVEGERPSVLAYKYGITKHTIRGNIMRFVEKAGGEGRARKLIALVKQSNAKVSPIVYKSDGMYTCLLCNEKLDEGKLEKHITTKHKAELQRAINYIMSKVEGKKKQEEANKKEVVVNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VL0</accession>
    <name>Q97VL0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peroxiredoxin, bacterioferritin comigratory protein homolog (Bcp-4)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">bcp-4</name>
        <name evidence="5" type="ordered locus">SSO2613</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2009-05" db="PDB data bank">
            <title>Exploring the catalytic mechanism of the first dimeric Bcp: functional, structural and docking analysis.</title>
            <authorList>
                <person name="Limauro D."/>
                <person name="D'Ambrosio K."/>
                <person name="Pedone E."/>
                <person name="Langella E."/>
                <person name="Galdi I."/>
                <person name="Pedone C."/>
                <person name="De Simone G."/>
                <person name="Bartolucci S."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90434">
        <property type="entry name" value="G90434"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989334.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3HJP">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.55 A"/>
        <property type="chains" value="A/B/C/D=1-156"/>
    </dbReference>
    <dbReference type="PDBsum" id="3HJP"/>
    <dbReference type="AlphaFoldDB" id="Q97VL0"/>
    <dbReference type="SMR" id="Q97VL0"/>
    <dbReference type="FunCoup" id="Q97VL0">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2613"/>
    <dbReference type="PaxDb" id="273057-SSO2613"/>
    <dbReference type="EnsemblBacteria" id="AAK42734">
        <property type="protein sequence ID" value="AAK42734"/>
        <property type="gene ID" value="SSO2613"/>
    </dbReference>
    <dbReference type="GeneID" id="1454061"/>
    <dbReference type="KEGG" id="sso:SSO2613"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2694"/>
    <dbReference type="eggNOG" id="arCOG00310">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_14_2_2"/>
    <dbReference type="InParanoid" id="Q97VL0"/>
    <dbReference type="PhylomeDB" id="Q97VL0"/>
    <dbReference type="BRENDA" id="1.11.1.24">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97VL0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03018">
        <property type="entry name" value="PRX_AhpE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050455">
        <property type="entry name" value="Tpx_Peroxidase_subfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43110">
        <property type="entry name" value="THIOL PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43110:SF1">
        <property type="entry name" value="THIOL PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="7" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="2"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="3">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="3HJP"/>
        </source>
    </evidence>
    <sequence length="156" mass="17461" checksum="D435645BFB8ED9FB" modified="2001-10-01" version="1">MVEIGEKAPEIELVDTDLKKVKIPSDFKGKVVVLAFYPAAFTSVCTKEMCTFRDSMAKFNEVNAVVIGISVDPPFSNKAFKEQNKINFTIVSDFNREAVKAYGVAGELPILKGYVLAKRSVFVIDKNGIVRYKWVSEDPTKEPNYDEIKDVVTKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VL5</accession>
    <name>Q97VL5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2608</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90434">
        <property type="entry name" value="B90434"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989327.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VL5"/>
    <dbReference type="PaxDb" id="273057-SSO2608"/>
    <dbReference type="EnsemblBacteria" id="AAK42729">
        <property type="protein sequence ID" value="AAK42729"/>
        <property type="gene ID" value="SSO2608"/>
    </dbReference>
    <dbReference type="GeneID" id="1454056"/>
    <dbReference type="KEGG" id="sso:SSO2608"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2689"/>
    <dbReference type="eggNOG" id="arCOG08527">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_782163_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VL5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="357" mass="42490" checksum="B01754B9AA429EC4" modified="2001-10-01" version="1">MPRSADIRLTPISESLRERAEVIMRMYGIEDEKDFYTNALMTMMNMMEKDDSLLGIDLCFLIPYILIHENFSFIHSSYIEDKVDVYSGDEIIGYLFKKYFNFMNPIHLKHLLEYLIPSPYTPLVVTNRNTLIYKISLNNINKKLRDYVQNFLENIVTVLGIGKINSSKDNIEIIAPYQSLIDFGNRNHHLVRVESSVISKRNQIITKFTHNNLKNNDKYDKLINDWSMYQLLTIYDTLFKNNLPLYNILDRLNDIYTLFLELRKTGHILYPYIRGIEYINYRLIDKEIINKNLDIFTLLIDPRIKLIKINNQEFIISGLTDFDENLSSFIRHVLDEPEIEKNNRFYFKIRMTKRIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VN0</accession>
    <name>Q97VN0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90432">
        <property type="entry name" value="C90432"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989300.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VN0"/>
    <dbReference type="STRING" id="273057.SSO2590"/>
    <dbReference type="PaxDb" id="273057-SSO2590"/>
    <dbReference type="DNASU" id="1454041"/>
    <dbReference type="EnsemblBacteria" id="AAK42714">
        <property type="protein sequence ID" value="AAK42714"/>
        <property type="gene ID" value="SSO2590"/>
    </dbReference>
    <dbReference type="GeneID" id="1454041"/>
    <dbReference type="KEGG" id="sso:SSO2590"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2671"/>
    <dbReference type="eggNOG" id="arCOG03769">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147147_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VN0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="123" mass="13017" checksum="15A5A14FFB3AACA8" modified="2001-10-01" version="1">MPLIVGLIGSVIAFIVAWIVVSIPTWIAGKIVTPRTATFGRAMLATLIGVIVYAIFTALFSLISPIIGVIIGFIAFLGVYKVVFNTGWLQALGIAILAIIVAFIILFILGLIGIALPHLGPFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VN6</accession>
    <name>Q97VN6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF83 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2582</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90431">
        <property type="entry name" value="D90431"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VN6"/>
    <dbReference type="STRING" id="273057.SSO2582"/>
    <dbReference type="PaxDb" id="273057-SSO2582"/>
    <dbReference type="EnsemblBacteria" id="AAK42707">
        <property type="protein sequence ID" value="AAK42707"/>
        <property type="gene ID" value="SSO2582"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2582"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2664"/>
    <dbReference type="eggNOG" id="arCOG08531">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369588_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VN6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="23821" checksum="AEC904D3509A632E" modified="2001-10-01" version="1">MSWFFGKIYLNSDQKSRMVENSLKKKLGYFINYKDIEYEVLSQYYILELRMPSNGKLGQLLHEYLQEYLINGIIRINEKYLPFYYNLNKALELLYEVVNERKLYYCDKRIERIGNIKLVGQADICSDDLVIEIKSKPELKKVDLMQALIYTFLYERDVILFMYGIYSGEYTIIKLPFNERNTNSLFEGLKKISEKEEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VQ3</accession>
    <name>Q97VQ3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nitroreductase, bluB homolog, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2560</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90429">
        <property type="entry name" value="B90429"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992733.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VQ3"/>
    <dbReference type="FunCoup" id="Q97VQ3">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2560"/>
    <dbReference type="PaxDb" id="273057-SSO2560"/>
    <dbReference type="EnsemblBacteria" id="AAK42689">
        <property type="protein sequence ID" value="AAK42689"/>
        <property type="gene ID" value="SSO2560"/>
    </dbReference>
    <dbReference type="GeneID" id="1454016"/>
    <dbReference type="KEGG" id="sso:SSO2560"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2642"/>
    <dbReference type="eggNOG" id="arCOG00288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070764_3_1_2"/>
    <dbReference type="InParanoid" id="Q97VQ3"/>
    <dbReference type="PhylomeDB" id="Q97VQ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012825">
        <property type="entry name" value="BluB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050627">
        <property type="entry name" value="Nitroreductase/BluB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02476">
        <property type="entry name" value="BluB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23026:SF90">
        <property type="entry name" value="IODOTYROSINE DEIODINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23026">
        <property type="entry name" value="NADPH NITROREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="4">
        <location>
            <begin position="7"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="227" mass="26444" checksum="8232D03916E23FE9" modified="2001-10-01" version="1">MDVYEAIRKRRDIRSYYKPDPIPDEVLAKILSAAHMAPSVGYSQPWNFIIIRDDEIRKKIKQVVEEERKRFESVLDEERKLIFSKIKVEAILDTPVNMAVTYDPNRFGPNILGRSTMPETSLYSTVLAIGNLWLAATAEGIGVGFVSFFNKEKVKEILNIPKEIELVSYLTLGYVREFPKIPELEEKGWNRRLMLSDLVFEDIFGKKTNNNFRLLLDRTFEEIFNNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VS8</accession>
    <name>Q97VS8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">YjbQ family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2004-11" db="PDB data bank">
            <title>Crystal structure of hypothetical protein (13815834) from Sulfolobus solfataricus at 1.76 A resolution.</title>
            <authorList>
                <consortium name="Joint Center for Structural Genomics (JCSG)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS)</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90425">
        <property type="entry name" value="G90425"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990979.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1VPH">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.76 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-137"/>
    </dbReference>
    <dbReference type="PDBsum" id="1VPH"/>
    <dbReference type="AlphaFoldDB" id="Q97VS8"/>
    <dbReference type="SMR" id="Q97VS8"/>
    <dbReference type="STRING" id="273057.SSO2532"/>
    <dbReference type="PaxDb" id="273057-SSO2532"/>
    <dbReference type="EnsemblBacteria" id="AAK42662">
        <property type="protein sequence ID" value="AAK42662"/>
        <property type="gene ID" value="SSO2532"/>
    </dbReference>
    <dbReference type="GeneID" id="1453989"/>
    <dbReference type="KEGG" id="sso:SSO2532"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2609"/>
    <dbReference type="eggNOG" id="arCOG04214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096980_1_2_2"/>
    <dbReference type="InParanoid" id="Q97VS8"/>
    <dbReference type="PhylomeDB" id="Q97VS8"/>
    <dbReference type="BRENDA" id="2.5.1.3">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97VS8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047_YjbQ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJBQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615:SF8">
        <property type="entry name" value="UPF0047 PROTEIN C4A8.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="1VPH"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15575" checksum="9055F50356305A8F" modified="2001-10-01" version="1">MKVYFDDIYVSTARQFELVDITDQVEQIVEKSGIKNGICLIFVAHSTAAIVANEHERGLMEDILTKIKEFTEPSRSWKHNLIDDNAHAHLGATFLGAERVFPVREGKLVRGTWQNIFLVELDGPRSERHITVEILGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VT5</accession>
    <name>Q97VT5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carbon monoxide dehydrogenase subunit G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2524</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90424">
        <property type="entry name" value="H90424"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988473.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VT5"/>
    <dbReference type="PaxDb" id="273057-SSO2524"/>
    <dbReference type="EnsemblBacteria" id="AAK42655">
        <property type="protein sequence ID" value="AAK42655"/>
        <property type="gene ID" value="SSO2524"/>
    </dbReference>
    <dbReference type="GeneID" id="1453982"/>
    <dbReference type="KEGG" id="sso:SSO2524"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2601"/>
    <dbReference type="eggNOG" id="arCOG01927">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146456_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VT5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05018">
        <property type="entry name" value="CoxG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010419">
        <property type="entry name" value="CO_DH_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06240">
        <property type="entry name" value="COXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16575" checksum="99E1F1103210F270" modified="2001-10-01" version="1">MEFKGTFDVKRTIEAVKGFLINPKQFAECLTGLQSYETNNNVFKAIFKLDVSQMKIPHMTTLTASINANIIEKENSVEINGDGRSAGVGIKFNIILGLKKDEGITRVNWFAKINLGMLSRLLGEENIRRIAEPNINHIINCISSKLSY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-13" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W05</accession>
    <name>RL13E_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L13e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl13e</name>
        <name type="ordered locus">SSO2442</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL13 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK42585" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42585.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90416">
        <property type="entry name" value="B90416"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993130.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W05"/>
    <dbReference type="SMR" id="Q97W05"/>
    <dbReference type="FunCoup" id="Q97W05">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2442"/>
    <dbReference type="PaxDb" id="273057-SSO2442"/>
    <dbReference type="EnsemblBacteria" id="AAK42585">
        <property type="protein sequence ID" value="AAK42585"/>
        <property type="gene ID" value="SSO2442"/>
    </dbReference>
    <dbReference type="GeneID" id="1453912"/>
    <dbReference type="KEGG" id="sso:SSO2442"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2523"/>
    <dbReference type="eggNOG" id="arCOG01013">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2165378_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W05"/>
    <dbReference type="PhylomeDB" id="Q97W05"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00499">
        <property type="entry name" value="Ribosomal_eL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001380">
        <property type="entry name" value="Ribosomal_eL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018256">
        <property type="entry name" value="Ribosomal_eL13_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008914">
        <property type="entry name" value="PRK12277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01294">
        <property type="entry name" value="Ribosomal_L13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01104">
        <property type="entry name" value="RIBOSOMAL_L13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000192941" description="Large ribosomal subunit protein eL13">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="79" mass="9356" checksum="30C2BA439A089645" modified="2001-12-13" version="2">MEFPKAVIKRPNYHFEYPHKRKYKRIGRGFSVGELEKAGLNINKARKLGIFVDIRRKSVHEENVETLKKFSEQLSNQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W25</accession>
    <name>Q97W25_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA family ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2420</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90413">
        <property type="entry name" value="F90413"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989409.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W25"/>
    <dbReference type="STRING" id="273057.SSO2420"/>
    <dbReference type="PaxDb" id="273057-SSO2420"/>
    <dbReference type="EnsemblBacteria" id="AAK42565">
        <property type="protein sequence ID" value="AAK42565"/>
        <property type="gene ID" value="SSO2420"/>
    </dbReference>
    <dbReference type="GeneID" id="1453892"/>
    <dbReference type="KEGG" id="sso:SSO2420"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2499"/>
    <dbReference type="eggNOG" id="arCOG01308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_8_3_2"/>
    <dbReference type="InParanoid" id="Q97W25"/>
    <dbReference type="PhylomeDB" id="Q97W25"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004129">
        <property type="entry name" value="AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000661">
        <property type="entry name" value="calmodulin-interacting protein 111 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="94"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="377"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="607" mass="68397" checksum="B549BE5BB02FE918" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W45</accession>
    <name>Q97W45_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-phosphopantoate--beta-alanine ligase</fullName>
            <ecNumber evidence="5">6.3.2.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Phosphopantothenate synthetase</fullName>
            <shortName evidence="5">PPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2399</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the condensation of (R)-4-phosphopantoate and beta-alanine to 4'-phosphopantothenate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(R)-4-phosphopantoate + beta-alanine + ATP = (R)-4'-phosphopantothenate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27930"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.2.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal phosphopantothenate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.36" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90411">
        <property type="entry name" value="B90411"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989440.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W45"/>
    <dbReference type="SMR" id="Q97W45"/>
    <dbReference type="FunCoup" id="Q97W45">
        <property type="interactions" value="105"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2399"/>
    <dbReference type="PaxDb" id="273057-SSO2399"/>
    <dbReference type="EnsemblBacteria" id="AAK42545">
        <property type="protein sequence ID" value="AAK42545"/>
        <property type="gene ID" value="SSO2399"/>
    </dbReference>
    <dbReference type="GeneID" id="1453872"/>
    <dbReference type="KEGG" id="sso:SSO2399"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2480"/>
    <dbReference type="eggNOG" id="arCOG04262">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078701_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W45"/>
    <dbReference type="PhylomeDB" id="Q97W45"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12640">
        <property type="entry name" value="Phosphopantoate/pantothenate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02224">
        <property type="entry name" value="PPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002855">
        <property type="entry name" value="PPS/PS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038138">
        <property type="entry name" value="PPS/PS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041123">
        <property type="entry name" value="phpantohe_syn_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010324">
        <property type="entry name" value="PRK13761.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40695">
        <property type="entry name" value="4-PHOSPHOPANTOATE--BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40695:SF1">
        <property type="entry name" value="4-PHOSPHOPANTOATE--BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02006">
        <property type="entry name" value="PPS_PS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004853">
        <property type="entry name" value="UCP004853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="194"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="200"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="212"/>
            <end position="213"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="266" mass="29660" checksum="28D971F0E19041E3" modified="2001-10-01" version="1">MDKAQDSKPWSIRDLIPENHPRRESLLIREKLIEAMEKSILVPQGLIAHGRGECFDYLIGEKTQDFAEKAIEAAAATLLLAKTPTISVNGNMAALVPEGLIRLAEESNAKLEINLFYRDERREKAIAEVLYKGGAKEVLGVGEDASEVIPELFSQRRRVSPKGIYKADVVLLGLEDGDRTEALVKMGKKVIAIDINPLSRTSRTATITIVDNIIRAVPRLVEKVKELKSKSKEELEQIVFNYDNKTILAESLKFIANRLTQLSLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W60</accession>
    <name>Q97W60_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA-t(6)A37 methylthiotransferase</fullName>
            <ecNumber evidence="11">2.8.4.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">SSO2380</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37075"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:11092"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:74420"/>
            <dbReference type="EC" id="2.8.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 1 or 2 [4Fe-4S] cluster. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the methylthiotransferase family. CDKAL1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.5" evidence="11"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99409">
        <property type="entry name" value="A99409"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989462.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W60"/>
    <dbReference type="FunCoup" id="Q97W60">
        <property type="interactions" value="304"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2380"/>
    <dbReference type="PaxDb" id="273057-SSO2380"/>
    <dbReference type="EnsemblBacteria" id="AAK42528">
        <property type="protein sequence ID" value="AAK42528"/>
        <property type="gene ID" value="SSO2380"/>
    </dbReference>
    <dbReference type="GeneID" id="1453855"/>
    <dbReference type="KEGG" id="sso:SSO2380"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2462"/>
    <dbReference type="eggNOG" id="arCOG01358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_4_2_2"/>
    <dbReference type="InParanoid" id="Q97W60"/>
    <dbReference type="PhylomeDB" id="Q97W60"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035598">
        <property type="term" value="F:tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035600">
        <property type="term" value="P:tRNA methylthiolation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12160:FF:000003">
        <property type="entry name" value="CDK5 regulatory subunit-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.30.20:FF:000002">
        <property type="entry name" value="threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006466">
        <property type="entry name" value="MiaB-like_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01578">
        <property type="entry name" value="MiaB-like-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00089">
        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918">
        <property type="entry name" value="RADICAL SAM PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918:SF45">
        <property type="entry name" value="THREONYLCARBAMOYLADENOSINE TRNA METHYLTHIOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="13">
        <location>
            <begin position="2"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="14">
        <location>
            <begin position="127"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="12">
        <location>
            <begin position="361"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAK42528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="421" mass="47263" checksum="9F6D78AD435927C9" modified="2001-10-01" version="1">MKNIYVETYGCALNRADTYVMMTLLKSEGYNFVENPENADIIILNTCAVRLETEERMKQRIKELNTLSKKLVVAGCMSSAEPATVLSIAPNASLIGPQSVERIVDIVKSEERKIVIEGDRALITPRTFDGKIAIIPVADGCAGNCSFCITKLARRKLRSYPLREIVNAARDAIQSGAREIELTGQDTAAYGLDLGGSISLVDVVNKVADIDGDFMIRIGMMTPEQAMRIMDDLIEAIRNPKVYKFIHLPVQSGDDRVLKLMNRKYTVDEYKELISEIRHKIPFVNITTDIIVGHPGEDEEAFNNTLLLMKELRFERIHLAMYSIRPNTRSASMPQVPDSIKKKRIQIANKLYEDLALSIHLDYVGSTSRVITTELGRKGSVIGRLMNYIPVVIRSENVELGKWYDVKITEASFYDLRGILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W76</accession>
    <name>Q97W76_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Succinate dehydrogenase subunit D (SdhD)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sdhD</name>
        <name evidence="1" type="ordered locus">SSO2359</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99407">
        <property type="entry name" value="A99407"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989490.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="PaxDb" id="273057-SSO2359"/>
    <dbReference type="EnsemblBacteria" id="AAK42512">
        <property type="protein sequence ID" value="AAK42512"/>
        <property type="gene ID" value="SSO2359"/>
    </dbReference>
    <dbReference type="GeneID" id="1453839"/>
    <dbReference type="KEGG" id="sso:SSO2359"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2446"/>
    <dbReference type="eggNOG" id="arCOG05375">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2177971_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W76"/>
    <dbReference type="BRENDA" id="1.3.5.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="121" mass="14236" checksum="A2E754B5689BEF49" modified="2001-10-01" version="1">MSEEIKKVIQDIGGKPDEWVNVSERPGKEPFAKELNYSLEDYFWGKVHVRNEGDIYVLVISKDVFNWKERIKDLKLNGNVIDAAGGLMWIQEFNIQGLKRDFEFLKTFIEDIRKQKQQKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WA7</accession>
    <name>Q97WA7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90403">
        <property type="entry name" value="B90403"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992899.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WA7"/>
    <dbReference type="STRING" id="273057.SSO2328"/>
    <dbReference type="PaxDb" id="273057-SSO2328"/>
    <dbReference type="EnsemblBacteria" id="AAK42481">
        <property type="protein sequence ID" value="AAK42481"/>
        <property type="gene ID" value="SSO2328"/>
    </dbReference>
    <dbReference type="GeneID" id="1453808"/>
    <dbReference type="KEGG" id="sso:SSO2328"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2414"/>
    <dbReference type="eggNOG" id="arCOG07318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1640039_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WA7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="162" mass="18303" checksum="D3A22E98726DEBEA" modified="2001-10-01" version="1">MELINVSLLYLVTGNYTHYLMLITILNVSKNFAIINMTEISHNITLSYVVAYPIFLLYIPNVTQQSIVTQFEGISTTIVNILNFTIYVDVYNSIVVHYIGNGINVTLIGATIPLGPGVYKPIPSYILGHQRTGSTVEEYEIFLLTIVLVVSFYVIFERMNKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WE5</accession>
    <name>GLCAE_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">D-glucosamine-6-phosphate 4-epimerase</fullName>
            <ecNumber evidence="2">5.1.3.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2281</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the synthesis of UDP-N-acetylgalactosamine (UDP-GalNAc). Catalyzes the conversion of glucosamine-6-phosphate (GlcN-6-P) to galactosamine-6-phosphate (GalN-6-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>D-glucosamine 6-phosphate = D-galactosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:18789"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:71674"/>
            <dbReference type="EC" id="5.1.3.42"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PGI/PMI family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.42" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90398">
        <property type="entry name" value="D90398"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991835.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WE5"/>
    <dbReference type="SMR" id="Q97WE5"/>
    <dbReference type="FunCoup" id="Q97WE5">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2281"/>
    <dbReference type="PaxDb" id="273057-SSO2281"/>
    <dbReference type="EnsemblBacteria" id="AAK42443">
        <property type="protein sequence ID" value="AAK42443"/>
        <property type="gene ID" value="SSO2281"/>
    </dbReference>
    <dbReference type="GeneID" id="1453770"/>
    <dbReference type="KEGG" id="sso:SSO2281"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2374"/>
    <dbReference type="eggNOG" id="arCOG00052">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059687_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WE5"/>
    <dbReference type="PhylomeDB" id="Q97WE5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004476">
        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05637">
        <property type="entry name" value="SIS_PGI_PMI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019490">
        <property type="entry name" value="Glu6P/Mann6P_isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02128">
        <property type="entry name" value="G6PI_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006422">
        <property type="entry name" value="PRK08674.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10432">
        <property type="entry name" value="bact-PGI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000227796" description="D-glucosamine-6-phosphate 4-epimerase">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="3">
        <location>
            <begin position="12"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q8ZWV0"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q96YC2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="296" mass="33141" checksum="E4EA89F3BA2F215B" modified="2001-10-01" version="1">MDEIYLNWDKMFDEAFRIPIHEIKNDNIVFSGIGGSGIVGEIANILGVELSFKRKFTGKESLIAVSYSGTTSETINDVENAVKAGSEVIIITSGGILEKLAKEKSLKLIKIPSGFQTRYAFPYLFTPLIKMTTKRRGIKINEMELKEGVIEAKEQIKADATRLAELLINRIPVIYSSKYLAIAKRFKQEINENAKHPAFYGEIPEINHNEIESYVHGPSLVSVIVESSEIDKITEDVLNSIVIKPYFNSDEKNISSLLALAGVTSLEMAKKLNEKPDKLYNIPKARQLTSNLFKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WF2</accession>
    <name>Q97WF2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dihydrodipicolinate synthase (DapA-1)</fullName>
            <ecNumber evidence="4">4.3.3.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dapA-1</name>
        <name evidence="4" type="ordered locus">SSO2274</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.3.3.7" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90397">
        <property type="entry name" value="E90397"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991846.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WF2"/>
    <dbReference type="FunCoup" id="Q97WF2">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2274"/>
    <dbReference type="PaxDb" id="273057-SSO2274"/>
    <dbReference type="EnsemblBacteria" id="AAK42436">
        <property type="protein sequence ID" value="AAK42436"/>
        <property type="gene ID" value="SSO2274"/>
    </dbReference>
    <dbReference type="GeneID" id="1453763"/>
    <dbReference type="KEGG" id="sso:SSO2274"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2366"/>
    <dbReference type="eggNOG" id="arCOG04172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_5_1_2"/>
    <dbReference type="InParanoid" id="Q97WF2"/>
    <dbReference type="PhylomeDB" id="Q97WF2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008675">
        <property type="term" value="F:2-dehydro-3-deoxy-phosphogluconate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00408">
        <property type="entry name" value="DHDPS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128:SF66">
        <property type="entry name" value="4-HYDROXY-2-OXOGLUTARATE ALDOLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="DIHYDRODIPICOLINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="2">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="289" mass="32745" checksum="53F025F9C071A891" modified="2001-10-01" version="1">MKENISALVTPYDDKENINTEALQQLLDFLIKKGIKDFWVLGTSGEFNMLSQDERILVVSKIREMVKGKIYVGVNENSTRNSLILAKKYYDLGVDYIFSVPPIYHKPSEKGLISYFSELRNAIDLPLFIYNIPSFTGYNVPLHIIEKLAREYVLDGMKYSTTDFVSFLKYLKALKGVNKNFKVFIGEDRMILSALILDTDGAVSGISNLVPELVTNLFLEFDRGNIQRAIEIQRILNKLVDVVSLGDYPSGIKIGLRYRGINVGSVRKPLLEDSRAEGEIYNVLKELGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WI1</accession>
    <name>Q97WI1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoglycerate mutase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2236</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90393">
        <property type="entry name" value="F90393"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WI1"/>
    <dbReference type="FunCoup" id="Q97WI1">
        <property type="interactions" value="269"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2236"/>
    <dbReference type="PaxDb" id="273057-SSO2236"/>
    <dbReference type="EnsemblBacteria" id="AAK42405">
        <property type="protein sequence ID" value="AAK42405"/>
        <property type="gene ID" value="SSO2236"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2236"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2332"/>
    <dbReference type="eggNOG" id="arCOG01991">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_9_3_2"/>
    <dbReference type="InParanoid" id="Q97WI1"/>
    <dbReference type="PhylomeDB" id="Q97WI1"/>
    <dbReference type="BRENDA" id="5.4.2.11">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054929">
        <property type="entry name" value="dPGM_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038349">
        <property type="entry name" value="dPGM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF1">
        <property type="entry name" value="HISTIDINE PHOSPHATASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="16"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="216" mass="24678" checksum="8A885F3C68299CF9" modified="2001-10-01" version="1">MEKYNNYVMTIIIFIRHGQSTSNVSKILSHDTNTYPLTEEGINQAKEAGKELTKLKVEKIYTSPVLRAYQTALIIGEVLGTLPIVDQRLRERFLGELNNTSFDPNDHWKLKVFKKQIEIKGIESWEDITKRMKSFLESVINKDNRIITAVSHSDPIRAIITYLLDMDDISGWGIRIPNASFTILRCDNNIDSCRIISIGSPLLTQQILSKLDINII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WI2</accession>
    <name>DTDA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="1 2">3.1.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>D-tyrosyl-tRNA(Tyr) deacylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dtdA</name>
        <name type="ordered locus">SSO2234</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="J. Biol. Chem." volume="281" first="27575" last="27585">
            <title>Identification in archaea of a novel D-Tyr-tRNATyr deacylase.</title>
            <authorList>
                <person name="Ferri-Fioni M.-L."/>
                <person name="Fromant M."/>
                <person name="Bouin A.-P."/>
                <person name="Aubard C."/>
                <person name="Lazennec C."/>
                <person name="Plateau P."/>
                <person name="Blanquet S."/>
            </authorList>
            <dbReference type="PubMed" id="16844682"/>
            <dbReference type="DOI" id="10.1074/jbc.M605860200"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-5</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <comment type="function">
        <text evidence="2">D-aminoacyl-tRNA deacylase with broad substrate specificity. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo. Catalyzes the hydrolysis of D-tyrosyl-tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>D-tyrosyl-tRNA(Tyr) + H2O = D-tyrosine + tRNA(Tyr)</text>
            <dbReference type="Rhea" id="RHEA:25347"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="Rhea" id="RHEA-COMP:9872"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58570"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78723"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtdA deacylase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="1 2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90393">
        <property type="entry name" value="E90393"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992037.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WI2"/>
    <dbReference type="SMR" id="Q97WI2"/>
    <dbReference type="FunCoup" id="Q97WI2">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2234"/>
    <dbReference type="PaxDb" id="273057-SSO2234"/>
    <dbReference type="EnsemblBacteria" id="AAK42404">
        <property type="protein sequence ID" value="AAK42404"/>
        <property type="gene ID" value="SSO2234"/>
    </dbReference>
    <dbReference type="GeneID" id="1453731"/>
    <dbReference type="KEGG" id="sso:SSO2234"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2331"/>
    <dbReference type="eggNOG" id="arCOG01616">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056464_1_0_2"/>
    <dbReference type="InParanoid" id="Q97WI2"/>
    <dbReference type="PhylomeDB" id="Q97WI2"/>
    <dbReference type="BRENDA" id="3.1.1.96">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051499">
        <property type="term" value="F:D-aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051500">
        <property type="term" value="F:D-tyrosyl-tRNA(Tyr) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106026">
        <property type="term" value="F:Gly-tRNA(Ala) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.50:FF:000002">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10700">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.50">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00562">
        <property type="entry name" value="Deacylase_DtdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018033">
        <property type="entry name" value="Deacylase_DtdA_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007508">
        <property type="entry name" value="DtdA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003070">
        <property type="entry name" value="PRK03995.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667:SF1">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04414">
        <property type="entry name" value="tRNA_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016210">
        <property type="entry name" value="UCP016210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142535">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000158974" description="D-aminoacyl-tRNA deacylase">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16844682"/>
        </source>
    </evidence>
    <sequence length="238" mass="26959" checksum="4C269C09E22D369A" modified="2001-10-01" version="1">MDIKLVYSTLDPVGVTIKKLGYRFEEINEDVTDFHYENGEAIVIFSRHESKAGIPSLTVHYPGNPSEEIMGGEPKKLGIAYPRLLTSILREIKKIDLNIEKTMEATHHGPTYQNVPVIFVEVGSNETYWTNDTIVKALVDSTIRSIDKVDEIDCEYYISGFGGPHYSRLFTKLADESCIGHVISKHYIDKLDDKVIIQTITNSVNTINKVVIDSLNSKQKERIIASLKKLNNINIEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WR5</accession>
    <name>Q97WR5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Extradiol ring-cleavage dioxygenase class III enzyme subunit B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2052</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DODA-type extradiol aromatic ring-opening dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90372">
        <property type="entry name" value="F90372"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WR5"/>
    <dbReference type="STRING" id="273057.SSO2052"/>
    <dbReference type="PaxDb" id="273057-SSO2052"/>
    <dbReference type="EnsemblBacteria" id="AAK42237">
        <property type="protein sequence ID" value="AAK42237"/>
        <property type="gene ID" value="SSO2052"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2052"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2132"/>
    <dbReference type="eggNOG" id="arCOG01730">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046582_2_1_2"/>
    <dbReference type="InParanoid" id="Q97WR5"/>
    <dbReference type="PhylomeDB" id="Q97WR5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07363">
        <property type="entry name" value="45_DOPA_Dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014436">
        <property type="entry name" value="Extradiol_dOase_DODA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004183">
        <property type="entry name" value="Xdiol_dOase_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30096">
        <property type="entry name" value="4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30096:SF0">
        <property type="entry name" value="4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02900">
        <property type="entry name" value="LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006157">
        <property type="entry name" value="Doxgns_DODA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53213">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Extradiol ring-cleavage dioxygenase class III enzyme subunit B" evidence="6">
        <location>
            <begin position="10"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="262" mass="30381" checksum="AAA7DAE9999ECD4E" modified="2001-10-01" version="1">MEFLLMTIGLFISHGSPTILIDENKWKDLLRRVGKEIEEKYKPETIIVSSPHFVSWTETFYIESSEKLECIQDYYDFPDETYKYCYNALNDTELVEEIVKNAEGKVVKDDKWGLDHGAWIPLFYMFPEYKPKVVTISITDNSPESHYEIGEKIRKAVEKLGRKAVFLATGSPTHRLDLFYFKITPKPTKFDAILMDLIKSGEFEKILKISELYPKEYQTAMPEGNLNTLHMLLGYIKPKKAEILGYDTPWPGVSMLAASFYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WR9</accession>
    <name>Q97WR9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2047</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99372">
        <property type="entry name" value="B99372"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923753.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WR9"/>
    <dbReference type="FunCoup" id="Q97WR9">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2047"/>
    <dbReference type="EnsemblBacteria" id="AAK42233">
        <property type="protein sequence ID" value="AAK42233"/>
        <property type="gene ID" value="SSO2047"/>
    </dbReference>
    <dbReference type="GeneID" id="7805892"/>
    <dbReference type="KEGG" id="sso:SSO2047"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2128"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_34_0_2"/>
    <dbReference type="InParanoid" id="Q97WR9"/>
    <dbReference type="PhylomeDB" id="Q97WR9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17504">
        <property type="entry name" value="MFS_MMR_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000025">
        <property type="entry name" value="Major facilitator superfamily MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF46">
        <property type="entry name" value="BLR6921 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="403"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="9"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="472" mass="51486" checksum="619DEF1D8421A1EC" modified="2001-10-01" version="1">MDSKEAMRTLITLTLMLTLVNYVETMVIPALPKIETQFSTTATTVAWITSAYLIVGAIASPLFGKLGDRYGKKKVYLLSIGFYSLAVLLAGFSTNIYFLIFARAIQGLGYATFPLAIAIITDLFPKERVAWAQGILSATLAAGPALGLLVGSYIVQDLGWPYAFHTAFLLSIILVIISAKYIVEIPEKTREKIDYLGATFLMLTVVPLLVYLSNGPNVGWTSLSQLLLIAISVIAFPIFLLVERRANEPLMRLELFGVRNIMVANIAGLISGAGMFLMFTGLVYYLQLPQPYGLGLSIIESGLLMAPVALVMMIVGPIVGRLINATGPKPLLIIGSVISIFGYLLLDTFRYSVYEVVLDVMITAVGLVNIMIPLVNMVAVSLPEEQRGIGIGMNTLIRTIGSSIGPVISTVFMDTYTTWIIYDFNDQIVPLAQVPSYSAFHYMYTAAVGIMVLNLIVALFTRNYVIKTGQHA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WX5</accession>
    <name>CMR7A_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR system CMR subunit Cmr7 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cmr7A</name>
        <name type="ordered locus">SSO1986</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="Mol. Cell" volume="45" first="303" last="313">
            <title>Structure and mechanism of the CMR complex for CRISPR-mediated antiviral immunity.</title>
            <authorList>
                <person name="Zhang J."/>
                <person name="Rouillon C."/>
                <person name="Kerou M."/>
                <person name="Reeks J."/>
                <person name="Brugger K."/>
                <person name="Graham S."/>
                <person name="Reimann J."/>
                <person name="Cannone G."/>
                <person name="Liu H."/>
                <person name="Albers S.V."/>
                <person name="Naismith J.H."/>
                <person name="Spagnolo L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="22227115"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2011.12.013"/>
        </citation>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION IN CMR COMPLEX</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="J. Struct. Funct. Genomics" volume="11" first="167" last="180">
            <title>The Scottish Structural Proteomics Facility: targets, methods and outputs.</title>
            <authorList>
                <person name="Oke M."/>
                <person name="Carter L.G."/>
                <person name="Johnson K.A."/>
                <person name="Liu H."/>
                <person name="McMahon S.A."/>
                <person name="Yan X."/>
                <person name="Kerou M."/>
                <person name="Weikart N.D."/>
                <person name="Kadi N."/>
                <person name="Sheikh M.A."/>
                <person name="Schmelz S."/>
                <person name="Dorward M."/>
                <person name="Zawadzki M."/>
                <person name="Cozens C."/>
                <person name="Falconer H."/>
                <person name="Powers H."/>
                <person name="Overton I.M."/>
                <person name="van Niekerk C.A."/>
                <person name="Peng X."/>
                <person name="Patel P."/>
                <person name="Garrett R.A."/>
                <person name="Prangishvili D."/>
                <person name="Botting C.H."/>
                <person name="Coote P.J."/>
                <person name="Dryden D.T."/>
                <person name="Barton G.J."/>
                <person name="Schwarz-Linek U."/>
                <person name="Challis G.L."/>
                <person name="Taylor G.L."/>
                <person name="White M.F."/>
                <person name="Naismith J.H."/>
            </authorList>
            <dbReference type="PubMed" id="20419351"/>
            <dbReference type="DOI" id="10.1007/s10969-010-9090-y"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). The CMR complex degrades RNA complementary to the crRNA (target RNA) within UA dinucleotides, generating 3'-OH and 5'-phosphate ends. Activity is dependent on the 8 nt long 5' tag in the crRNA, an unpaired 3' flag on the target RNA, and is stimulated by ATP. Some cleavage of the guide crRNA can also be observed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Possible homodimer. Part of the CMR ribonucleoprotein complex, consisting of crRNA plus Cmr1/Cmr2/Cmr3/Cmr4/Cmr5/Cmr6 at 1:1 and possibly 3 Cmr7 dimers. A Cmr2/Cmr3/Cmr7 subcomplex without crRNA can also be isolated. It does not cleave target RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR system Cmr7 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90365">
        <property type="entry name" value="A90365"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993002.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2X5Q">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.05 A"/>
        <property type="chains" value="A/B=1-197"/>
    </dbReference>
    <dbReference type="PDBsum" id="2X5Q"/>
    <dbReference type="AlphaFoldDB" id="Q97WX5"/>
    <dbReference type="SMR" id="Q97WX5"/>
    <dbReference type="STRING" id="273057.SSO1986"/>
    <dbReference type="PaxDb" id="273057-SSO1986"/>
    <dbReference type="EnsemblBacteria" id="AAK42176">
        <property type="protein sequence ID" value="AAK42176"/>
        <property type="gene ID" value="SSO1986"/>
    </dbReference>
    <dbReference type="GeneID" id="1453503"/>
    <dbReference type="GeneID" id="27428312"/>
    <dbReference type="KEGG" id="sso:SSO1986"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2062"/>
    <dbReference type="eggNOG" id="arCOG08552">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1472150_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WX5"/>
    <dbReference type="EvolutionaryTrace" id="Q97WX5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099048">
        <property type="term" value="P:CRISPR-cas system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043959">
        <property type="entry name" value="Cmr7A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053743">
        <property type="entry name" value="CRISPR_Cmr7_comp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19021">
        <property type="entry name" value="Cmr7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418083" description="CRISPR system CMR subunit Cmr7 1">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="10"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="20"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="23"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="26"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="30"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="34"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="44"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="51"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="55"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="61"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="65"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="69"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="94"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="107"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="115"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="138"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="158"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="163"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="171"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="187"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="22227115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2X5Q"/>
        </source>
    </evidence>
    <sequence length="197" mass="22151" checksum="C8AC8DACA9BA65D6" modified="2001-10-01" version="1">MTSGAGWEEQVFLPITNSISSEDNNQIKIGSSVSIEYNQNGQHVSQIDDKGLHNILVLTGYAIDESTGELVPTFDPCDYVKGILISGKILKGNHFKIIGIPSNKLYIIRKKDVHGNITFSLPIKNFNTGTYQVDLRDKVTSFVSLDRDVAKTIVDNVLAKIYAKIYNSLNKEQKDKLYRDVEEIFNYYSIKSLKSNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X01</accession>
    <name>Q97X01_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1959</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90361">
        <property type="entry name" value="G90361"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991752.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X01"/>
    <dbReference type="STRING" id="273057.SSO1959"/>
    <dbReference type="PaxDb" id="273057-SSO1959"/>
    <dbReference type="EnsemblBacteria" id="AAK42150">
        <property type="protein sequence ID" value="AAK42150"/>
        <property type="gene ID" value="SSO1959"/>
    </dbReference>
    <dbReference type="GeneID" id="15296865"/>
    <dbReference type="KEGG" id="sso:SSO1959"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2033"/>
    <dbReference type="eggNOG" id="arCOG05710">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117974_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X01"/>
    <dbReference type="PhylomeDB" id="Q97X01"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="158" mass="18558" checksum="07E6600DDAC7AE0D" modified="2001-10-01" version="1">MPRELTPIEQLQLLLPGYRGYKAKDLIRQDDFLVRRAVIDRLEQTIRIISEKESFIASTDPFSPQLKVMEFLISKIRELINNIMSSQGGGADIYARWKVTTEELDQIVNHDMKMIDIASQLVELAKNNRIEEFEPLLNDLRAVFYDRMKLFYPPELRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X71</accession>
    <name>Q97X71_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Terminal quinol oxidase, subunit II, putative (DoxA-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">doxA-like</name>
        <name evidence="3" type="ordered locus">SSO1882</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90351">
        <property type="entry name" value="H90351"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991416.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X71"/>
    <dbReference type="STRING" id="273057.SSO1882"/>
    <dbReference type="PaxDb" id="273057-SSO1882"/>
    <dbReference type="EnsemblBacteria" id="AAK42071">
        <property type="protein sequence ID" value="AAK42071"/>
        <property type="gene ID" value="SSO1882"/>
    </dbReference>
    <dbReference type="GeneID" id="1453398"/>
    <dbReference type="KEGG" id="sso:SSO1882"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1935"/>
    <dbReference type="eggNOG" id="arCOG05342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1544265_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X71"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011636">
        <property type="entry name" value="DoxA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07680">
        <property type="entry name" value="DoxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Thiosulphate:quinone oxidoreductase small subunit DoxA" evidence="2">
        <location>
            <begin position="29"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="173" mass="18902" checksum="FDC53C259476A28A" modified="2001-10-01" version="1">MNGKIITLVAIIMIIFTTGVTLGLYQINFQGLGNLTNYSKTPAYSLVGTRLYENGTLFLQVARTAGPDTYGGFVVLVQLLYSNGTVLYQWTGQQLGMLSSNNIKNEFPLHPSKPIKIENYGVGIEVPLGQNSTIILQLPHPIPSGTYIIKVWDADGQSTAYGNKFQITVKVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X87</accession>
    <name>Q97X87_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH bat-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1868</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90349">
        <property type="entry name" value="H90349"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988462.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X87"/>
    <dbReference type="STRING" id="273057.SSO1868"/>
    <dbReference type="PaxDb" id="273057-SSO1868"/>
    <dbReference type="EnsemblBacteria" id="AAK42055">
        <property type="protein sequence ID" value="AAK42055"/>
        <property type="gene ID" value="SSO1868"/>
    </dbReference>
    <dbReference type="GeneID" id="1453382"/>
    <dbReference type="KEGG" id="sso:SSO1868"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1918"/>
    <dbReference type="eggNOG" id="arCOG02271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101251_1_0_2"/>
    <dbReference type="InParanoid" id="Q97X87"/>
    <dbReference type="PhylomeDB" id="Q97X87"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF2">
        <property type="entry name" value="HTH BAT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH bat-type" evidence="1">
        <location>
            <begin position="145"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="204" mass="23425" checksum="41756C76AE382F35" modified="2001-10-01" version="1">MLKSPFEATILIEEHPCEVMKIISSTGLKGVVDNVKLGDNTTDHIVLFEKEVQKDDLIKLKSHSTKVLRLNDNKIWVRTYGCAVCKILYTSNVVVEKIKVVRERTLLYTLLIPNTMALKEFLASLISQDIEFTVLSTSEISSNELTDRQMEILKLAYKMGYFDDDRRVTLTELAKQLGISTPTLEEILRRALRKVVKFYLDKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XB9</accession>
    <name>Q97XB9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1825</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90345">
        <property type="entry name" value="G90345"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923612.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XB9"/>
    <dbReference type="PaxDb" id="273057-SSO1825"/>
    <dbReference type="EnsemblBacteria" id="AAK42022">
        <property type="protein sequence ID" value="AAK42022"/>
        <property type="gene ID" value="SSO1825"/>
    </dbReference>
    <dbReference type="GeneID" id="1454801"/>
    <dbReference type="KEGG" id="sso:SSO1825"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1885"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154343_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XB9"/>
    <dbReference type="PhylomeDB" id="Q97XB9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="147" mass="16698" checksum="6B2F28A7A9C55C04" modified="2001-10-01" version="1">MGFNDVFLLMGYTTSKLKNFTAFRKYKGSWGRKHGKSYFGFKVCNLVERRTNFVRDFKVGLANLSDLAFSFDNVKLMADRAWISRKDVLVKGVGSARLPVEGSGVKIREGKASSTTLRGVVIEVFFLNLYRDLEILSTRIRTKVLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XC1</accession>
    <name>Q97XC1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1823</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90345">
        <property type="entry name" value="E90345"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XC1"/>
    <dbReference type="PaxDb" id="273057-SSO1823"/>
    <dbReference type="EnsemblBacteria" id="AAK42020">
        <property type="protein sequence ID" value="AAK42020"/>
        <property type="gene ID" value="SSO1823"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1823"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1883"/>
    <dbReference type="eggNOG" id="arCOG07205">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2127953_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XC1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="113" mass="13114" checksum="92D6B7CF16555AB7" modified="2001-10-01" version="1">MISLTKWRASSYINCLKDYFNDNKLVSSMAFLIAASKGDSLYVFAPDTDCIIYTEELITDVKGRCEEYVKLFSSYKQDIIKSASLKLWHYYANKKVEFTDEEKKLLSQLGIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XD2</accession>
    <name>Q97XD2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8979</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90344">
        <property type="entry name" value="B90344"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XD2"/>
    <dbReference type="PaxDb" id="273057-SSO8979"/>
    <dbReference type="EnsemblBacteria" id="AAK42009">
        <property type="protein sequence ID" value="AAK42009"/>
        <property type="gene ID" value="SSO8979"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8979"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1871"/>
    <dbReference type="eggNOG" id="arCOG08435">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2662440_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XD2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="81" mass="9858" checksum="C60D19E3A4982D07" modified="2001-10-01" version="1">MMIRYIIDTRVLELPHYEIIERKIDEIRESEGIVIISEVDPIRVLARLKIQKKVDIIVRLIHKNNQEEKKWIIYLIKSSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XE1</accession>
    <name>Q97XE1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ferredoxin (Carboxy-end) (Zfx-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">zfx-2</name>
        <name evidence="9" type="ordered locus">SSO8958</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90343">
        <property type="entry name" value="A90343"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XE1"/>
    <dbReference type="STRING" id="273057.SSO8958"/>
    <dbReference type="PaxDb" id="273057-SSO8958"/>
    <dbReference type="EnsemblBacteria" id="AAK42000">
        <property type="protein sequence ID" value="AAK42000"/>
        <property type="gene ID" value="SSO8958"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8958"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1862"/>
    <dbReference type="eggNOG" id="arCOG04548">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_5_2"/>
    <dbReference type="InParanoid" id="Q97XE1"/>
    <dbReference type="PhylomeDB" id="Q97XE1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF6">
        <property type="entry name" value="L-ASPARTATE SEMIALDEHYDE SULFURTRANSFERASE IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="32"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="61" mass="6633" checksum="703DC0A1BAA04B0D" modified="2001-10-01" version="1">MLKSDNVSCITACPVNVFRWFDTPGNPVSEKKADPFNEQACIFCMACVNVCPVAAIDVKPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XF4</accession>
    <name>Q97XF4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1786</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90340">
        <property type="entry name" value="E90340"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XF4"/>
    <dbReference type="STRING" id="273057.SSO1786"/>
    <dbReference type="PaxDb" id="273057-SSO1786"/>
    <dbReference type="EnsemblBacteria" id="AAK41980">
        <property type="protein sequence ID" value="AAK41980"/>
        <property type="gene ID" value="SSO1786"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1786"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1841"/>
    <dbReference type="eggNOG" id="arCOG00727">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121774_1_0_2"/>
    <dbReference type="InParanoid" id="Q97XF4"/>
    <dbReference type="PhylomeDB" id="Q97XF4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09873">
        <property type="entry name" value="PIN_Pae0151-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044153">
        <property type="entry name" value="PIN_Pae0151-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051619">
        <property type="entry name" value="TypeII_TA_RNase_PINc/VapC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901:SF1">
        <property type="entry name" value="EXONUCLEASE VAPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901">
        <property type="entry name" value="RIBONUCLEASE VAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="2">
        <location>
            <begin position="8"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="143" mass="16487" checksum="BE2D3ED705243315" modified="2001-10-01" version="1">MERQELAVVDASVVIKWFVNENYSKEALILKEAYVKGLEDLLAPCILPFEVLNGLKYTYSLGEKELEEVGKILSDFQITLYGFENILDEMVSLSLRYGITIYDAAYIALGKVLNEKVYTADERLIRKVKELPFVIHIKDYKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XJ6</accession>
    <name>Q97XJ6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO8813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90335">
        <property type="entry name" value="A90335"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990357.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XJ6"/>
    <dbReference type="SMR" id="Q97XJ6"/>
    <dbReference type="PaxDb" id="273057-SSO8813"/>
    <dbReference type="DNASU" id="1454715"/>
    <dbReference type="EnsemblBacteria" id="AAK41936">
        <property type="protein sequence ID" value="AAK41936"/>
        <property type="gene ID" value="SSO8813"/>
    </dbReference>
    <dbReference type="GeneID" id="1454715"/>
    <dbReference type="KEGG" id="sso:SSO8813"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1783"/>
    <dbReference type="eggNOG" id="arCOG00713">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2550444_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XJ6"/>
    <dbReference type="PhylomeDB" id="Q97XJ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052106">
        <property type="entry name" value="PINc/VapC_TA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38826">
        <property type="entry name" value="RIBONUCLEASE VAPC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38826:SF5">
        <property type="entry name" value="RIBONUCLEASE VAPC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="21"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="90" mass="10475" checksum="D06FA46E18EC50E3" modified="2001-10-01" version="1">MRLFTLSKKKYNVKFEDTIDFLDKIILPYTIILPINSHDYEKAKGIMISKTLKPSDAFHVAVMINNSIKKIVSEDSDFDKINGIERIWVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XK8</accession>
    <name>CMR7B_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Putative CRISPR system CMR subunit Cmr7 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cmr7b</name>
        <name type="ordered locus">SSO1725</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="Mol. Cell" volume="45" first="303" last="313">
            <title>Structure and mechanism of the CMR complex for CRISPR-mediated antiviral immunity.</title>
            <authorList>
                <person name="Zhang J."/>
                <person name="Rouillon C."/>
                <person name="Kerou M."/>
                <person name="Reeks J."/>
                <person name="Brugger K."/>
                <person name="Graham S."/>
                <person name="Reimann J."/>
                <person name="Cannone G."/>
                <person name="Liu H."/>
                <person name="Albers S.V."/>
                <person name="Naismith J.H."/>
                <person name="Spagnolo L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="22227115"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2011.12.013"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.08 ANGSTROMS) OF 2-190</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR system Cmr7 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90333">
        <property type="entry name" value="E90333"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923682.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2XVO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.08 A"/>
        <property type="chains" value="A/B/C/D=3-190"/>
    </dbReference>
    <dbReference type="PDBsum" id="2XVO"/>
    <dbReference type="AlphaFoldDB" id="Q97XK8"/>
    <dbReference type="SMR" id="Q97XK8"/>
    <dbReference type="STRING" id="273057.SSO1725"/>
    <dbReference type="PaxDb" id="273057-SSO1725"/>
    <dbReference type="EnsemblBacteria" id="AAK41924">
        <property type="protein sequence ID" value="AAK41924"/>
        <property type="gene ID" value="SSO1725"/>
    </dbReference>
    <dbReference type="GeneID" id="1454703"/>
    <dbReference type="KEGG" id="sso:SSO1725"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1768"/>
    <dbReference type="HOGENOM" id="CLU_1425150_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XK8"/>
    <dbReference type="EvolutionaryTrace" id="Q97XK8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055112">
        <property type="entry name" value="Cmr7b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053743">
        <property type="entry name" value="CRISPR_Cmr7_comp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22392">
        <property type="entry name" value="Cmr7b-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418084" description="Putative CRISPR system CMR subunit Cmr7 2">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="12"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="23"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="31"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="41"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="45"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="51"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="55"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="59"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="63"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="84"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="107"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="119"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="124"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="127"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="140"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="156"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="164"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="179"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="187"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0000305" key="3">
        <source>
            <dbReference type="PubMed" id="22227115"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2XVO"/>
        </source>
    </evidence>
    <sequence length="190" mass="21446" checksum="91925FE7CEE20F3A" modified="2001-10-01" version="1">MSSPGGSQQVEWVFIPVIKDVTYEFKVDNNDNITELYVNGNKLGPASSLEMDFYFDVDVSNNQVRKFNNVFVLFGVIATKDSNKIKMQLTLNPCDFVRGFVFPSQDPSQLNNIFASNNKVSVSEKAFAILNRKKEGAVSSTINVYITQNTYTGNTKIEKIQQNTIIIEKNTGIVFKIPNDMLNIFRYSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XL1</accession>
    <name>Q97XL1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1048</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1717</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99333">
        <property type="entry name" value="A99333"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XL1"/>
    <dbReference type="STRING" id="273057.SSO1717"/>
    <dbReference type="PaxDb" id="273057-SSO1717"/>
    <dbReference type="EnsemblBacteria" id="AAK41920">
        <property type="protein sequence ID" value="AAK41920"/>
        <property type="gene ID" value="SSO1717"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1717"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1764"/>
    <dbReference type="eggNOG" id="arCOG02130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056788_15_2_2"/>
    <dbReference type="InParanoid" id="Q97XL1"/>
    <dbReference type="PhylomeDB" id="Q97XL1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="6"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="175" mass="20260" checksum="0CE34A75AEBB53DE" modified="2001-10-01" version="1">MERKALFFLDECRTVISTSIKKVLAKVGSKPVMRVNIGFSSIYVILAINAWTGEVVVSLAKRPNSESVKYFLRYFKRRVGSGRVYMVMDNYSPHKTKGTLEVCRRKGIHPVFTPPYSPELNMAEAVFKSLKNYMSNKIFYTIEDVKNCIKQFFKENKYRFNLNAITYLGLDKIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XP9</accession>
    <name>Q97XP9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-methylhydantoinase B (HuyB-1)</fullName>
            <ecNumber evidence="2">3.5.2.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">huyB-1</name>
        <name evidence="2" type="ordered locus">SSO1662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.14" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90327">
        <property type="entry name" value="C90327"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990097.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XP9"/>
    <dbReference type="STRING" id="273057.SSO1662"/>
    <dbReference type="PaxDb" id="273057-SSO1662"/>
    <dbReference type="EnsemblBacteria" id="AAK41874">
        <property type="protein sequence ID" value="AAK41874"/>
        <property type="gene ID" value="SSO1662"/>
    </dbReference>
    <dbReference type="GeneID" id="1454653"/>
    <dbReference type="KEGG" id="sso:SSO1662"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1711"/>
    <dbReference type="eggNOG" id="arCOG01512">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020413_1_1_2"/>
    <dbReference type="InParanoid" id="Q97XP9"/>
    <dbReference type="PhylomeDB" id="Q97XP9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047423">
        <property type="term" value="F:N-methylhydantoinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF23">
        <property type="entry name" value="HYPOTHETICAL 5-OXOPROLINASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="583" mass="63396" checksum="72AAA3BB94AF361F" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XU9</accession>
    <name>Q97XU9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vitamin K epoxide reductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">SSO1608</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VKOR family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90320">
        <property type="entry name" value="F90320"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923510.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XU9"/>
    <dbReference type="FunCoup" id="Q97XU9">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1608"/>
    <dbReference type="PaxDb" id="273057-SSO1608"/>
    <dbReference type="DNASU" id="1454600"/>
    <dbReference type="EnsemblBacteria" id="AAK41821">
        <property type="protein sequence ID" value="AAK41821"/>
        <property type="gene ID" value="SSO1608"/>
    </dbReference>
    <dbReference type="GeneID" id="1454600"/>
    <dbReference type="KEGG" id="sso:SSO1608"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1648"/>
    <dbReference type="eggNOG" id="arCOG03749">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1782568_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XU9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12918">
        <property type="entry name" value="VKOR_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.130">
        <property type="entry name" value="VKOR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012932">
        <property type="entry name" value="VKOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038354">
        <property type="entry name" value="VKOR_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011479">
        <property type="entry name" value="PRK14889.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07884">
        <property type="entry name" value="VKOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00756">
        <property type="entry name" value="VKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0874">Quinone</keyword>
    <keyword evidence="9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Vitamin K epoxide reductase" evidence="11">
        <location>
            <begin position="6"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK41821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="145" mass="16083" checksum="B736BEC60C6C67D1" modified="2001-10-01" version="1">MIILKGNKIDYLFLLFGLLGLSTMIYSYLSFSLDVISRNSCTINSVISCSSVENSIYSTFLGIKLYYYGMAYFSAIIVLSLLVVKGTVKNIIGYVISLLSIIASIISVYLIYTEIFLLGHMCIVCTLAHVSIFSVLILSVIKFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y08</accession>
    <name>Q97Y08_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1545</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2009" name="Proteins" volume="74" first="1041" last="1049">
            <title>Crystal structure of a novel archaeal AAA+ ATPase SSO1545 from Sulfolobus solfataricus.</title>
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                <person name="Xu Q."/>
                <person name="Rife C.L."/>
                <person name="Carlton D."/>
                <person name="Miller M.D."/>
                <person name="Krishna S.S."/>
                <person name="Elsliger M.A."/>
                <person name="Abdubek P."/>
                <person name="Astakhova T."/>
                <person name="Chiu H.J."/>
                <person name="Clayton T."/>
                <person name="Duan L."/>
                <person name="Feuerhelm J."/>
                <person name="Grzechnik S.K."/>
                <person name="Hale J."/>
                <person name="Han G.W."/>
                <person name="Jaroszewski L."/>
                <person name="Jin K.K."/>
                <person name="Klock H.E."/>
                <person name="Knuth M.W."/>
                <person name="Kumar A."/>
                <person name="McMullan D."/>
                <person name="Morse A.T."/>
                <person name="Nigoghossian E."/>
                <person name="Okach L."/>
                <person name="Oommachen S."/>
                <person name="Paulsen J."/>
                <person name="Reyes R."/>
                <person name="van den Bedem H."/>
                <person name="Hodgson K.O."/>
                <person name="Wooley J."/>
                <person name="Deacon A.M."/>
                <person name="Godzik A."/>
                <person name="Lesley S.A."/>
                <person name="Wilson I.A."/>
            </authorList>
            <dbReference type="PubMed" id="19089981"/>
            <dbReference type="DOI" id="10.1002/prot.22325"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH ADP AND MG(2+)</scope>
        <scope>DISULFIDE BONDS</scope>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
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                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
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                <person name="Gordon P.M.K."/>
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        <citation type="journal article" date="2023" name="Curr. Res. Struct. Biol." volume="5" first="100098" last="100098">
            <title>Structure of the &lt;i&gt;Saccharolobus solfataricus&lt;/i&gt; type III-D CRISPR effector.</title>
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                <person name="Cannone G."/>
                <person name="Kompaniiets D."/>
                <person name="Graham S."/>
                <person name="White M.F."/>
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            <dbReference type="DOI" id="10.1016/j.crstbi.2023.100098"/>
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    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="75"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="180"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="8BMW"/>
        </source>
    </evidence>
    <sequence length="206" mass="23200" checksum="07FF3E3F411DCE36" modified="2001-10-01" version="1">MIPIKVSMRNLSSLTIAGGSTISSIDIPLNPLGVPPSTIKGTMRTAVHNLLPNGYTSCGEVEPESIREAHKNGVCDVCKLFGYPDSLTGCFTIDVSKTDYRTSYITRVSIDDKTQRAKEGSLFTQQIILPNSDISFTVYYNCNDERLFKLLLYSILDLRYWRLGRNTMIDVKVNNVEEICKSVKCDEEINGILNQLSRYLWEAENK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-04-18" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YC2</accession>
    <name>CAS2A_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated endoribonuclease Cas2 1</fullName>
            <ecNumber>3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>CAS2 endoribonuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cas21</name>
        <name type="ordered locus">SSO1404</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="J. Biol. Chem." volume="283" first="20361" last="20371">
            <title>A novel family of sequence-specific endoribonucleases associated with the clustered regularly interspaced short palindromic repeats.</title>
            <authorList>
                <person name="Beloglazova N."/>
                <person name="Brown G."/>
                <person name="Zimmerman M.D."/>
                <person name="Proudfoot M."/>
                <person name="Makarova K.S."/>
                <person name="Kudritska M."/>
                <person name="Kochinyan S."/>
                <person name="Wang S."/>
                <person name="Chruszcz M."/>
                <person name="Minor W."/>
                <person name="Koonin E.V."/>
                <person name="Edwards A.M."/>
                <person name="Savchenko A."/>
                <person name="Yakunin A.F."/>
            </authorList>
            <dbReference type="PubMed" id="18482976"/>
            <dbReference type="DOI" id="10.1074/jbc.M803225200"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.59 ANGSTROMS)</scope>
        <scope>FUNCTION AS A SSRNA-SPECIFIC ENDORIBONUCLEASE</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>PH-DEPENDENCE</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF TYR-9; ASP-10; ARG-17; ARG-19; ARG-31 AND PHE-37</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="J. Struct. Funct. Genomics" volume="11" first="167" last="180">
            <title>The Scottish Structural Proteomics Facility: targets, methods and outputs.</title>
            <authorList>
                <person name="Oke M."/>
                <person name="Carter L.G."/>
                <person name="Johnson K.A."/>
                <person name="Liu H."/>
                <person name="McMahon S.A."/>
                <person name="Yan X."/>
                <person name="Kerou M."/>
                <person name="Weikart N.D."/>
                <person name="Kadi N."/>
                <person name="Sheikh M.A."/>
                <person name="Schmelz S."/>
                <person name="Dorward M."/>
                <person name="Zawadzki M."/>
                <person name="Cozens C."/>
                <person name="Falconer H."/>
                <person name="Powers H."/>
                <person name="Overton I.M."/>
                <person name="van Niekerk C.A."/>
                <person name="Peng X."/>
                <person name="Patel P."/>
                <person name="Garrett R.A."/>
                <person name="Prangishvili D."/>
                <person name="Botting C.H."/>
                <person name="Coote P.J."/>
                <person name="Dryden D.T."/>
                <person name="Barton G.J."/>
                <person name="Schwarz-Linek U."/>
                <person name="Challis G.L."/>
                <person name="Taylor G.L."/>
                <person name="White M.F."/>
                <person name="Naismith J.H."/>
            </authorList>
            <dbReference type="PubMed" id="20419351"/>
            <dbReference type="DOI" id="10.1007/s10969-010-9090-y"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 2-101</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Involved in the integration of spacer DNA into the CRISPR cassette (By similarity). Functions as a ssRNA-specific endoribonuclease, producing a 5'-phosphomonoester and a 3'-hydroxy. Does not process pre-crRNA in the manner expected if it were the CRISPR-processing endoribonuclease. Prefers U-rich substrates and often cuts between adjacent U residues in regions predicted to be single-stranded. RNAs as short as 10 residues can serve as substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text>Optimum pH is 8.5-9.0.</text>
        </phDependence>
    </comment>
    <comment type="subunit">
        <text evidence="1 3">Forms a heterotetramer with a Cas1 homodimer (By similarity). Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CRISPR-associated endoribonuclease Cas2 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90297">
        <property type="entry name" value="H90297"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988403.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2I8E">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.59 A"/>
        <property type="chains" value="A=1-101"/>
    </dbReference>
    <dbReference type="PDB" id="2IVY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.40 A"/>
        <property type="chains" value="A=2-101"/>
    </dbReference>
    <dbReference type="PDBsum" id="2I8E"/>
    <dbReference type="PDBsum" id="2IVY"/>
    <dbReference type="AlphaFoldDB" id="Q97YC2"/>
    <dbReference type="SMR" id="Q97YC2"/>
    <dbReference type="STRING" id="273057.SSO1404"/>
    <dbReference type="PaxDb" id="273057-SSO1404"/>
    <dbReference type="EnsemblBacteria" id="AAK41639">
        <property type="protein sequence ID" value="AAK41639"/>
        <property type="gene ID" value="SSO1404"/>
    </dbReference>
    <dbReference type="GeneID" id="1454418"/>
    <dbReference type="KEGG" id="sso:SSO1404"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1420"/>
    <dbReference type="eggNOG" id="arCOG04194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161124_2_0_2"/>
    <dbReference type="InParanoid" id="Q97YC2"/>
    <dbReference type="PhylomeDB" id="Q97YC2"/>
    <dbReference type="BRENDA" id="3.1.26.12">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97YC2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09725">
        <property type="entry name" value="Cas2_I_II_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01471">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021127">
        <property type="entry name" value="CRISPR_associated_Cas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019199">
        <property type="entry name" value="Virulence_VapD/CRISPR_Cas2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01573">
        <property type="entry name" value="cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405:SF3">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS2 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09827">
        <property type="entry name" value="CRISPR_Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143430">
        <property type="entry name" value="TTP0101/SSO1404-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000416951" description="CRISPR-associated endoribonuclease Cas2 1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Considerable loss of RNase activity." evidence="3">
        <original>Y</original>
        <variation>A</variation>
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of RNase activity." evidence="3">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Considerable loss of RNase activity." evidence="3">
        <original>R</original>
        <variation>A</variation>
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Considerable loss of RNase activity." evidence="3">
        <original>R</original>
        <variation>A</variation>
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of RNase activity." evidence="3">
        <original>R</original>
        <variation>A</variation>
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of RNase activity." evidence="3">
        <original>F</original>
        <variation>A</variation>
        <location>
            <position position="37"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="3"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="30"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="36"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="68"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="77"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="18482976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="2IVY"/>
        </source>
    </evidence>
    <sequence length="101" mass="11936" checksum="A7C338AD76202E17" modified="2001-10-01" version="1">MAMLYLIFYDITDDNLRNRVAEFLKKKGLDRIQYSVFMGDLNSSRLKDVEAGLKIIGNRKKLQEDERFFILIVPITENQFRERIVIGYSGSEREEKSNVVW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2016-03-16" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YC8</accession>
    <name>CSA5A_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR type I-A cluster 1/Apern-associated protein Csa5-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">csa5</name>
        <name type="ordered locus">SSO1398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2013" name="RNA Biol." volume="10" first="762" last="769">
            <title>Structure of the archaeal Cascade subunit Csa5: relating the small subunits of CRISPR effector complexes.</title>
            <authorList>
                <person name="Reeks J."/>
                <person name="Graham S."/>
                <person name="Anderson L."/>
                <person name="Liu H."/>
                <person name="White M.F."/>
                <person name="Naismith J.H."/>
            </authorList>
            <dbReference type="PubMed" id="23846216"/>
            <dbReference type="DOI" id="10.4161/RNA.23854"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.72 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF ASP-35</scope>
    </reference>
    <comment type="function">
        <text evidence="2">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Oligomerizes as an infinite helical thread in crystal structure; disrupting a potential salt bridge between Asp-35 and Arg-55 leads to altered elution from a sizing column. By immunoprecipitation Csa5 interacts weakly with a Cas5/Cas7 complex. There are 3 csa5, 3 cas5 and 2 cas7 genes in this organism; the immunoprecipitation studies may not distinguish between them.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99297">
        <property type="entry name" value="B99297"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988390.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3ZC4">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.72 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I=1-162"/>
    </dbReference>
    <dbReference type="PDBsum" id="3ZC4"/>
    <dbReference type="AlphaFoldDB" id="Q97YC8"/>
    <dbReference type="SMR" id="Q97YC8"/>
    <dbReference type="STRING" id="273057.SSO1398"/>
    <dbReference type="PaxDb" id="273057-SSO1398"/>
    <dbReference type="EnsemblBacteria" id="AAK41633">
        <property type="protein sequence ID" value="AAK41633"/>
        <property type="gene ID" value="SSO1398"/>
    </dbReference>
    <dbReference type="GeneID" id="1454412"/>
    <dbReference type="GeneID" id="44130254"/>
    <dbReference type="KEGG" id="sso:SSO1398"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1414"/>
    <dbReference type="eggNOG" id="arCOG03823">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631728_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YC8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048642">
        <property type="entry name" value="Csa5_I-A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21681">
        <property type="entry name" value="Csa5_I-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000435656" description="CRISPR type I-A cluster 1/Apern-associated protein Csa5-1">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Altered protein oligomerization, protein has a smaller radius of gyration, elution from a sizing column is altered." evidence="1">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="31"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="35"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="65"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="75"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="91"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="98"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="103"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="106"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="115"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="120"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="160"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="23846216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="3ZC4"/>
        </source>
    </evidence>
    <sequence length="162" mass="18523" checksum="12622E57398E516B" modified="2001-10-01" version="1">MEASEPVAETISKRFWTLIKMLRFYVVLRRFGYIDPLIYSIDPKQIKDVLSEALREFVSYTSSSSSRSIVIYDDPKNPVTAQAPCLVVAKRDEIPQNFPSIYRYTIYKIDKSSEYCISPLVVNDKYATLITPNESVIKEFFDKLDSNIQYARVLASLAVGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YG5</accession>
    <name>Q97YG5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dolichyl-phosphate mannose synthase related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="B90292"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YG5"/>
    <dbReference type="FunCoup" id="Q97YG5">
        <property type="interactions" value="281"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1357"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1357"/>
    <dbReference type="EnsemblBacteria" id="AAK41593">
        <property type="protein sequence ID" value="AAK41593"/>
        <property type="gene ID" value="SSO1357"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1357"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1363"/>
    <dbReference type="eggNOG" id="arCOG00895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_9_0_2"/>
    <dbReference type="InParanoid" id="Q97YG5"/>
    <dbReference type="PhylomeDB" id="Q97YG5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="26"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="228" mass="26292" checksum="330E28FDDAB6DAF7" modified="2001-10-01" version="1">MLYKYYRSSPKILLLLTCKESLKMISVIIPAFNEERRIGKTLEKISSTLPNAEVVVVFDGHDNTPEVVKKFPVKLIISKERLGKGMALKRGITESNFQRVLLLDADFPITEEELNKILSTDADLVIPRRKIIGMPLKRRFLHKAFIVLTKILFPSLLKFSDFQAGVKLVNREKVVSVLDELIINDFLFDVNLIYAFKRRHYKIKEVEINYIHDESDSKISRGSVLKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YH7</accession>
    <name>Q97YH7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1160</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO7756</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK67519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YH7"/>
    <dbReference type="STRING" id="273057.SSO7756"/>
    <dbReference type="PaxDb" id="273057-SSO7756"/>
    <dbReference type="EnsemblBacteria" id="AAK67519">
        <property type="protein sequence ID" value="AAK67519"/>
        <property type="gene ID" value="SSO7756"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7756"/>
    <dbReference type="HOGENOM" id="CLU_3245536_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YH7"/>
    <dbReference type="PhylomeDB" id="Q97YH7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK67519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="42" mass="4748" checksum="B8CCF7543C2C2708" modified="2001-10-01" version="1">MTKDDTVKLLIEQISVNLITLNAGFYSADVLNFISKFKLQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YN6</accession>
    <name>Q97YN6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Solute-binding protein family 5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1288</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90283">
        <property type="entry name" value="G90283"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991993.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YN6"/>
    <dbReference type="STRING" id="273057.SSO1288"/>
    <dbReference type="PaxDb" id="273057-SSO1288"/>
    <dbReference type="EnsemblBacteria" id="AAK41526">
        <property type="protein sequence ID" value="AAK41526"/>
        <property type="gene ID" value="SSO1288"/>
    </dbReference>
    <dbReference type="GeneID" id="1454305"/>
    <dbReference type="KEGG" id="sso:SSO1288"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1287"/>
    <dbReference type="eggNOG" id="arCOG01673">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_370735_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YN6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.105.10:FF:000070">
        <property type="entry name" value="Extracellular solute-binding protein family 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000572">
        <property type="entry name" value="Extracellular solute-binding protein family 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290:SF9">
        <property type="entry name" value="OLIGOPEPTIDE-BINDING PROTEIN APPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="800"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="domain" description="Solute-binding protein family 5" evidence="5">
        <location>
            <begin position="59"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Solute-binding protein family 5" evidence="5">
        <location>
            <begin position="446"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="826" mass="91051" checksum="AE545D917B636F94" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YQ5</accession>
    <name>Q97YQ5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90281">
        <property type="entry name" value="C90281"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990026.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YQ5"/>
    <dbReference type="STRING" id="273057.SSO1269"/>
    <dbReference type="PaxDb" id="273057-SSO1269"/>
    <dbReference type="EnsemblBacteria" id="AAK41506">
        <property type="protein sequence ID" value="AAK41506"/>
        <property type="gene ID" value="SSO1269"/>
    </dbReference>
    <dbReference type="GeneID" id="7810279"/>
    <dbReference type="KEGG" id="sso:SSO1269"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1265"/>
    <dbReference type="eggNOG" id="arCOG01443">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1623534_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YQ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd10013">
        <property type="entry name" value="Cas3''_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="163" mass="19091" checksum="5A8E4CAD4F0458E5" modified="2001-10-01" version="1">MDLEKFRREYYYVVPIIANRIKKDEEKVKKYIELAIILQSAIGNEVYTAYYLYYIYEELGDIDLGIPIVLAIVNHRDVWKARALRSVTAYNTLKVFEVKDDVKRIIENKLREINIPIKIVMKILNNIEPIQIRKLFSNLSIVHPEVYSLILGVFTSIKQGNEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YU2</accession>
    <name>Q97YU2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90275">
        <property type="entry name" value="G90275"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923253.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YU2"/>
    <dbReference type="STRING" id="273057.SSO1215"/>
    <dbReference type="PaxDb" id="273057-SSO1215"/>
    <dbReference type="EnsemblBacteria" id="AAK41462">
        <property type="protein sequence ID" value="AAK41462"/>
        <property type="gene ID" value="SSO1215"/>
    </dbReference>
    <dbReference type="GeneID" id="1454241"/>
    <dbReference type="KEGG" id="sso:SSO1215"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1215"/>
    <dbReference type="HOGENOM" id="CLU_1096750_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YU2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="253" mass="29878" checksum="75E0229B05CB163C" modified="2001-10-01" version="1">MPSVSIWLSPKTYKYVEELANFLTKKPNRLIKEIIENKIVITENIESYYNVVKGLYKWYYYQGEILDNEKYIRRVLKRKNAEAILNIINLHDDIRVVFKTLGVLMLIVSLKSYAKIPEENFSTLKLIKYDLMEEVKRIRIYSLPLLYSKILWLRCVEKIRELSILKTKDWEKLAFTAAIYAVTILGEETPDSVYSHYNLKEFEKEWSELIKSSIKIMTEEENIIPRCTLCKNIVNGSRCSCGNSEIFYDDLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YW4</accession>
    <name>Q97YW4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1187</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90272">
        <property type="entry name" value="E90272"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YW4"/>
    <dbReference type="PaxDb" id="273057-SSO1187"/>
    <dbReference type="EnsemblBacteria" id="AAK41436">
        <property type="protein sequence ID" value="AAK41436"/>
        <property type="gene ID" value="SSO1187"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1187"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1186"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YW4"/>
    <dbReference type="PhylomeDB" id="Q97YW4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="177"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="302"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="393" mass="46022" checksum="5E399CA207742BB3" modified="2001-10-01" version="1">MPTLGFRFRAYTDEQTLRALKAQLKLTCEIYNTLRWADIYFYQRDGKGLTQTELRQLALDLRKQDDEYKQLYSQVVQQVADRYSEAKKRFFEGLARFPKEKKPHKYYSLVYTQSGWKILHVREIRKGKKNKKKLITLKLSNLGTFKVIVHRDFPLDKVKRVVVKLTRSERIYITFVVDHEFPKLPNTGKVVAIDVGVEKLLITSDGEYFPNLRPYEKALWKVKHIHRELSRKKFLSNNWFKAKVKLARAYEHLKNLRTDLYMKLGKWFAEHYDVVVMEGIHAKQLVGKSLRSLRRRLSDVGFGELRGVLKYQLEKYGKKLILVNPAYTSKTCARCGYVKNDLSLSDRVFVCPNCGWIADRDYNASLNILRGSGSERPLVWSSALYQYSGKVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YW8</accession>
    <name>Q97YW8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Inorganic phosphate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1183</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99272">
        <property type="entry name" value="A99272"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YW8"/>
    <dbReference type="FunCoup" id="Q97YW8">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1183"/>
    <dbReference type="PaxDb" id="273057-SSO1183"/>
    <dbReference type="EnsemblBacteria" id="AAK41432">
        <property type="protein sequence ID" value="AAK41432"/>
        <property type="gene ID" value="SSO1183"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1183"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1180"/>
    <dbReference type="eggNOG" id="arCOG02689">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_2_2"/>
    <dbReference type="InParanoid" id="Q97YW8"/>
    <dbReference type="PhylomeDB" id="Q97YW8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="22"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="357" mass="38790" checksum="9C0698324784FCD5" modified="2001-10-01" version="1">MNVKNVFKPLDEAKFGWFHAKSLITTGMGVFTDGYDLSSIGIVLLSVLASFGITKNSPDYVITTSLITGAALIGATIGAILFGFLSNMGRKKFYGIDVTLMTFGALLQAFVQNPLELIIVRFLLGLGIGADYVLSPTIMAEHSNAKDRGKTLVLGFGLFWGFGASTAAGLYLILQTMGLPTDLIWRIVLAAGSIPAASVIYLRRKIPETARYLGRIKGDLNELKNVIKEVTRLEVSNVKDIKDNVKLTEYFIANWKLILTAAILWFLFDIVAYAGILFGPSLIANSLGMNSGTFQLLIEGAFTIPGGLIALTLIDKLGRKPLQVIGVWASWDFMIFNINPQPLGFTEVADVHPSPPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z37</accession>
    <name>Q97Z37_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90262">
        <property type="entry name" value="H90262"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989735.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z37"/>
    <dbReference type="STRING" id="273057.SSO1102"/>
    <dbReference type="PaxDb" id="273057-SSO1102"/>
    <dbReference type="EnsemblBacteria" id="AAK41359">
        <property type="protein sequence ID" value="AAK41359"/>
        <property type="gene ID" value="SSO1102"/>
    </dbReference>
    <dbReference type="GeneID" id="89231099"/>
    <dbReference type="KEGG" id="sso:SSO1102"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1099"/>
    <dbReference type="eggNOG" id="arCOG00023">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176005_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z37"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="106" mass="12276" checksum="0F3A4E81B565FC60" modified="2001-10-01" version="1">MRYSTYCEDGYINIVPALKVALIISLLSKGQPLREACRCVNMSITAYERHKKDSVEKIQKIREDKEISQMIDSLSSRIINKEKIDPMLFCLVCSKSRRLFNLPVCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z48</accession>
    <name>Q97Z48_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1089</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90261">
        <property type="entry name" value="E90261"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z48"/>
    <dbReference type="STRING" id="273057.SSO1089"/>
    <dbReference type="PaxDb" id="273057-SSO1089"/>
    <dbReference type="EnsemblBacteria" id="AAK41348">
        <property type="protein sequence ID" value="AAK41348"/>
        <property type="gene ID" value="SSO1089"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1089"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1086"/>
    <dbReference type="eggNOG" id="arCOG02900">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058577_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z48"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040929">
        <property type="entry name" value="ArnB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737:SF6">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18677">
        <property type="entry name" value="ArnB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="120"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="436" mass="50004" checksum="E85A0CC751D0AFBF" modified="2001-10-01" version="1">MLRELILICRLLLYRLLSACLLFNGRCWRLLYLLLWFFSTTFDAKEGIVRIFITTDWTSPCHVCIKGVIVLKNFYYNILDMAIAISLKQSHTYVYSDRPTEVGFIVYIVPQQGAITSSIHYIIMIDNSPSMRGEKLNTAVQSAQKLLYSLNEGDYVTLILFSNHPEIKYQGPAKGIITFDVGKGYTTRLHEAVNFALNLAKQSQVPNKIIMLTDGKPTDKRNVKDYEKFDIPPNTQIITIGIGSDYNERILKKLADKSSGKFYHLKDISELPDVFESQRTTSTYAHNLNLIVPQGFMPFNYDLPIRIPIVEKLIAVYGSFVIPAGKDPYTITFTADYIDPIDNQRKTVTKSVILQRGNPEVVESHLDRDVILEIRYYRLLREYSEALEAGKDTTKVLNELKRVADETRKPELIEETKKLSNDSKSDLSEITRKMRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z51</accession>
    <name>Q97Z51_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glutaconate CoA-transferase, subunit B (GctB)</fullName>
            <ecNumber evidence="1">2.8.3.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gctB</name>
        <name evidence="1" type="ordered locus">SSO1085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.3.12" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90261">
        <property type="entry name" value="B90261"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989719.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z51"/>
    <dbReference type="FunCoup" id="Q97Z51">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1085"/>
    <dbReference type="PaxDb" id="273057-SSO1085"/>
    <dbReference type="EnsemblBacteria" id="AAK41345">
        <property type="protein sequence ID" value="AAK41345"/>
        <property type="gene ID" value="SSO1085"/>
    </dbReference>
    <dbReference type="GeneID" id="1454124"/>
    <dbReference type="KEGG" id="sso:SSO1085"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1083"/>
    <dbReference type="eggNOG" id="arCOG05316">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069088_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z51"/>
    <dbReference type="PhylomeDB" id="Q97Z51"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018730">
        <property type="term" value="F:glutaconate CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43293">
        <property type="entry name" value="ACETATE COA-TRANSFERASE YDIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43293:SF3">
        <property type="entry name" value="CHOLESTEROL RING-CLEAVING HYDROLASE IPDB SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="245" mass="27742" checksum="094E0D3306A57427" modified="2001-10-01" version="1">MKSYNVDYVIKAISTLLEDGELVYIGLNSIPSLIASFMARDLYRKKIRIIGVAEASNPSRVVLSPSTGNPFFVEASPVMITADAFDLAQKGKLDVMFLGPAQIDEETNVNLTMIGNEYHNPKVKLPGGAATAFILPLSRKAILWNLKHSKRSLVRRVDFITGTAKFSNNKVFVVTNLGVLAYNREERKWYLEAIYPFSSYEKVVENTDFEVKRKDSERLIDLTDEDLRFINYMDPYNLRSALETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z85</accession>
    <name>Q97Z85_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GTPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1046</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90256">
        <property type="entry name" value="E90256"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z85"/>
    <dbReference type="FunCoup" id="Q97Z85">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1046"/>
    <dbReference type="PaxDb" id="273057-SSO1046"/>
    <dbReference type="EnsemblBacteria" id="AAK41308">
        <property type="protein sequence ID" value="AAK41308"/>
        <property type="gene ID" value="SSO1046"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1046"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1040"/>
    <dbReference type="eggNOG" id="arCOG01225">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037460_3_0_2"/>
    <dbReference type="InParanoid" id="Q97Z85"/>
    <dbReference type="PhylomeDB" id="Q97Z85"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010340">
        <property type="entry name" value="PRK13768.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010342">
        <property type="entry name" value="PRK13768.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF8">
        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="29253" checksum="FE877297016A6CCD" modified="2001-10-01" version="1">MKRDMYYVFILGTAGSGKTTLTKNLQDYLLDQEMDTAVINLDPAVEHLPYTPDFDVRDYVDAYEVMQNYHLGPNSSLIASIDLILTKASEIKSEIDQIEANYVLVDTPGQIELFAYRDTGRLISQLIRGNNKALGLFLLDSFLAKEARSFISLLLLSSSIKFRLDLPIINILNKIDLLTEKELEQILAWGDNAENLIDELGRLDEYSLELVNLLIESLSYNLIPMSSEEGKGFNELYAEIQRVIAGGEDYLTEESNPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZC1</accession>
    <name>Q97ZC1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sugar ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">SSO1001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. MalFG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90252">
        <property type="entry name" value="A90252"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989237.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZC1"/>
    <dbReference type="FunCoup" id="Q97ZC1">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1001"/>
    <dbReference type="TCDB" id="3.A.1.1.15">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1001"/>
    <dbReference type="EnsemblBacteria" id="AAK41272">
        <property type="protein sequence ID" value="AAK41272"/>
        <property type="gene ID" value="SSO1001"/>
    </dbReference>
    <dbReference type="GeneID" id="1455239"/>
    <dbReference type="KEGG" id="sso:SSO1001"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.998"/>
    <dbReference type="eggNOG" id="arCOG00159">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_2_2"/>
    <dbReference type="InParanoid" id="Q97ZC1"/>
    <dbReference type="PhylomeDB" id="Q97ZC1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050901">
        <property type="entry name" value="BP-dep_ABC_trans_perm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32243">
        <property type="entry name" value="MALTOSE TRANSPORT SYSTEM PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32243:SF50">
        <property type="entry name" value="MALTOSE_MALTODEXTRIN TRANSPORT SYSTEM PERMEASE PROTEIN MALG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="65"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK41272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="267" mass="29127" checksum="EE7B5CBFCCB0CF96" modified="2001-10-01" version="1">MRLWVYLGAIVVGIYFLFPLYILVLLAFNSPKYTVLAKFPSLLPVSLTLNNLLTALQGTAFIDPFIKSLETATLVGIITIALAIPAGYGLSRLPRAIAYSIIILLLVTNMMPAIVIGIPIAVDFLKLHLFESVVGLALAQTLITLPLATFILQGTFSSIPIDLEHQARVDGANLFNRLFSVLLPLAAPGIAAAFLISWMFSWDEFTYAILLIPYHSTLPVTIYQDVTRGNLLAGIAFSLIFTLPVIILTFALQKYLRGEYLAGGIKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-30" modified="2025-06-18" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZE2</accession>
    <name>PYRH_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Uridylate kinase</fullName>
            <shortName>UK</shortName>
            <ecNumber>2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Uridine monophosphate kinase</fullName>
            <shortName>UMP kinase</shortName>
            <shortName>UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pyrH</name>
        <name type="ordered locus">SSO0976</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2007" name="Biochemistry" volume="46" first="2745" last="2757">
            <title>Structural and enzymatic investigation of the Sulfolobus solfataricus uridylate kinase shows competitive UTP inhibition and the lack of GTP stimulation.</title>
            <authorList>
                <person name="Jensen K.S."/>
                <person name="Johansson E."/>
                <person name="Jensen K.F."/>
            </authorList>
            <dbReference type="PubMed" id="17297917"/>
            <dbReference type="DOI" id="10.1021/bi0618159"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 2-227 IN COMPLEX WITH UMP; ATP ANALOG AND UTP</scope>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>REACTION MECHANISM</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible phosphorylation of UMP to UDP, with ATP as the most efficient phosphate donor. Is also able to phosphorylate dUMP, although much less efficiently.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>UMP + ATP = UDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Unlike most bacteria, is not activated by GTP. UTP acts as a competitive inhibitor against both substrates. High concentration of UMP abolishes the inhibition of UTP at low ATP concentrations, indicating that UTP binds to the acceptor site (UMP site).</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">14 uM for UMP (at pH 7.2)</KM>
            <KM evidence="2">81 uM for ATP (at pH 7.2)</KM>
            <Vmax evidence="2">5.0 umol/min/mg enzyme (at pH 5.5)</Vmax>
            <Vmax evidence="2">45.0 umol/min/mg enzyme (at pH 7.2)</Vmax>
            <Vmax evidence="2">12.0 umol/min/mg enzyme (at pH 8.5)</Vmax>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 7.0.</text>
        </phDependence>
    </comment>
    <comment type="pathway">
        <text>Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text>Catalysis proceeds by a sequential bi-bi reaction mechanism of random order.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UMP kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90249">
        <property type="entry name" value="C90249"/>
    </dbReference>
    <dbReference type="PDB" id="2J4J">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B/C/D/E/F=2-227"/>
    </dbReference>
    <dbReference type="PDB" id="2J4K">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B/C/D/E/F=2-227"/>
    </dbReference>
    <dbReference type="PDB" id="2J4L">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=2-227"/>
    </dbReference>
    <dbReference type="PDBsum" id="2J4J"/>
    <dbReference type="PDBsum" id="2J4K"/>
    <dbReference type="PDBsum" id="2J4L"/>
    <dbReference type="AlphaFoldDB" id="Q97ZE2"/>
    <dbReference type="SMR" id="Q97ZE2"/>
    <dbReference type="FunCoup" id="Q97ZE2">
        <property type="interactions" value="158"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0976"/>
    <dbReference type="PaxDb" id="273057-SSO0976"/>
    <dbReference type="DNASU" id="1455217"/>
    <dbReference type="EnsemblBacteria" id="AAK41250">
        <property type="protein sequence ID" value="AAK41250"/>
        <property type="gene ID" value="SSO0976"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0976"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.975"/>
    <dbReference type="eggNOG" id="arCOG00858">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079546_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZE2"/>
    <dbReference type="PhylomeDB" id="Q97ZE2"/>
    <dbReference type="BRENDA" id="2.7.4.22">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97ZE2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006225">
        <property type="term" value="P:UDP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04253">
        <property type="entry name" value="AAK_UMPK-PyrH-Pf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000030">
        <property type="entry name" value="Uridylate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_A">
        <property type="entry name" value="PyrH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011818">
        <property type="entry name" value="Uridylate_kinase_arch/spir"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02076">
        <property type="entry name" value="pyrH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42833">
        <property type="entry name" value="URIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42833:SF4">
        <property type="entry name" value="URIDYLATE KINASE PUMPKIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000143927" description="Uridylate kinase">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site">
        <location>
            <begin position="7"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="114"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="3"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="11"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="37"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="96"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="106"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="146"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="150"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="153"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="160"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="164"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="179"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="187"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="199"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="205"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="211"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="222"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="17297917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2J4J"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="2J4K"/>
        </source>
    </evidence>
    <sequence length="227" mass="25120" checksum="25FB40F5D19B34F2" modified="2001-10-01" version="1">MMNIILKISGKFFDEDNVDNLIVLRQSIKELADNGFRVGIVTGGGSTARRYIKLAREIGIGEAYLDLLGIWASRLNAYLVMFSLQDLAYMHVPQSLEEFIQDWSHGKVVVTGGFQPGQSTAAVAALVAEASSSKTLVVATNVDGVYEKDPRIYADVKLIPHLTTQDLRKILEGSQSVQAGTYELLDPLAIKIVERSKIRVIVMNYRKLNRIIDILKGEEVSSIIEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-10-02" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZR5</accession>
    <name>Q97ZR5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SSV1 virus orf B-129 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO6024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90198">
        <property type="entry name" value="C90198"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZR5"/>
    <dbReference type="STRING" id="273057.SSO6024"/>
    <dbReference type="PaxDb" id="273057-SSO6024"/>
    <dbReference type="EnsemblBacteria" id="AAK40842">
        <property type="protein sequence ID" value="AAK40842"/>
        <property type="gene ID" value="SSO6024"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO6024"/>
    <dbReference type="eggNOG" id="arCOG06947">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_189640_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZR5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054177">
        <property type="entry name" value="B129_C2H2-Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22034">
        <property type="entry name" value="B129_C2H2-zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="9"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="63" mass="7124" checksum="3A8259E35D9DC1E0" modified="2001-10-01" version="1">MTAKAVSPFVCPICLTPFSSSSALKQHIRYEEHGKECQICKKRFTTTDATLDHICKKHNICVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZS2</accession>
    <name>Q97ZS2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0507</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90196">
        <property type="entry name" value="D90196"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZS2"/>
    <dbReference type="STRING" id="273057.SSO0507"/>
    <dbReference type="PaxDb" id="273057-SSO0507"/>
    <dbReference type="EnsemblBacteria" id="AAK40827">
        <property type="protein sequence ID" value="AAK40827"/>
        <property type="gene ID" value="SSO0507"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0507"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.502"/>
    <dbReference type="eggNOG" id="arCOG05923">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149110_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZS2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="127" mass="15201" checksum="E8D728A970ABA563" modified="2001-10-01" version="1">MRKAKSKNIFTLSSYYFMIKHAEYTRHGITEPLMLIMLYKKVEDGKIISAFRFSVYKNMIIIVYEEDKLSGGEVLDFDIYNMTNLINKIKKYYDESIDDIVIFGEKQYVDEFLNKFLSDEEEETEKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-02-12" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZX7</accession>
    <name>RPO12_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo12</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit P</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo12</name>
        <name evidence="1" type="synonym">rpoP</name>
        <name type="ordered locus">SSO5865</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="journal article" date="2008" name="Nature" volume="451" first="851" last="854">
            <title>The X-ray crystal structure of RNA polymerase from Archaea.</title>
            <authorList>
                <person name="Hirata A."/>
                <person name="Klein B.J."/>
                <person name="Murakami K.S."/>
            </authorList>
            <dbReference type="PubMed" id="18235446"/>
            <dbReference type="DOI" id="10.1038/nature06530"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF THE RNA POLYMERASE COMPLEX IN COMPLEX WITH ZINC</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2 3 4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1 2 3 4">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 13-subunit RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90189">
        <property type="entry name" value="C90189"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988725.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2PMZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="P/Z=1-48"/>
    </dbReference>
    <dbReference type="PDB" id="3HKZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="P/X=1-48"/>
    </dbReference>
    <dbReference type="PDBsum" id="2PMZ"/>
    <dbReference type="PDBsum" id="3HKZ"/>
    <dbReference type="AlphaFoldDB" id="Q97ZX7"/>
    <dbReference type="SMR" id="Q97ZX7"/>
    <dbReference type="DIP" id="DIP-60645N"/>
    <dbReference type="FunCoup" id="Q97ZX7">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="IntAct" id="Q97ZX7">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO5865"/>
    <dbReference type="PaxDb" id="273057-SSO5865"/>
    <dbReference type="EnsemblBacteria" id="AAK40770">
        <property type="protein sequence ID" value="AAK40770"/>
        <property type="gene ID" value="SSO5865"/>
    </dbReference>
    <dbReference type="GeneID" id="24760666"/>
    <dbReference type="KEGG" id="sso:SSO5865"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.438"/>
    <dbReference type="eggNOG" id="arCOG04341">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179456_2_0_2"/>
    <dbReference type="InParanoid" id="Q97ZX7"/>
    <dbReference type="PhylomeDB" id="Q97ZX7"/>
    <dbReference type="BRENDA" id="2.7.7.6">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97ZX7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.30">
        <property type="entry name" value="RNA polymerase ii, chain L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00615">
        <property type="entry name" value="RNApol_arch_Rpo12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006591">
        <property type="entry name" value="RNAP_P/RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023464">
        <property type="entry name" value="Rpo12"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001604">
        <property type="entry name" value="PRK00398.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00659">
        <property type="entry name" value="RPOLCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000159767" description="DNA-directed RNA polymerase subunit Rpo12">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 3 4">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 3 4">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 3 4">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 3 4">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="10"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="22"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="43"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18235446"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="3">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="4">
        <source>
            <dbReference type="PDB" id="3HKZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="3HKZ"/>
        </source>
    </evidence>
    <sequence length="48" mass="5597" checksum="C822E342B8BEFF21" modified="2001-10-01" version="1">MAVYRCGKCWKTFTDEQLKVLPGVRCPYCGYKIIFMVRKPTIKIVKAI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-10-11" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZY3</accession>
    <name>ENDA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-splicing endonuclease</fullName>
            <ecNumber evidence="1">4.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA-intron endonuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">endA</name>
        <name type="ordered locus">SSO0439</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.</text>
            <dbReference type="EC" id="4.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90188">
        <property type="entry name" value="E90188"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988732.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZY3"/>
    <dbReference type="SMR" id="Q97ZY3"/>
    <dbReference type="STRING" id="273057.SSO0439"/>
    <dbReference type="PaxDb" id="273057-SSO0439"/>
    <dbReference type="EnsemblBacteria" id="AAK40764">
        <property type="protein sequence ID" value="AAK40764"/>
        <property type="gene ID" value="SSO0439"/>
    </dbReference>
    <dbReference type="GeneID" id="1455574"/>
    <dbReference type="GeneID" id="44129418"/>
    <dbReference type="KEGG" id="sso:SSO0439"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.432"/>
    <dbReference type="eggNOG" id="arCOG01701">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114393_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZY3"/>
    <dbReference type="PhylomeDB" id="Q97ZY3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1350.10:FF:000006">
        <property type="entry name" value="tRNA-splicing endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.20">
        <property type="entry name" value="tRNA intron endonuclease, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01833">
        <property type="entry name" value="EndA_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006678">
        <property type="entry name" value="tRNA_intron_Endonuc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036740">
        <property type="entry name" value="tRNA_intron_Endonuc_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006676">
        <property type="entry name" value="tRNA_splic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016442">
        <property type="entry name" value="tRNA_splic_arch_short"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00324">
        <property type="entry name" value="endA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02778">
        <property type="entry name" value="tRNA_int_endo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005285">
        <property type="entry name" value="tRNA_splic_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55267">
        <property type="entry name" value="tRNA-intron endonuclease N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000109481" description="tRNA-splicing endonuclease">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01833"/>
        </source>
    </evidence>
    <sequence length="182" mass="20710" checksum="FC27F4A79BE12293" modified="2001-10-01" version="1">MVKALLVGSKVLVPSIDESRYLYSNGFYGKPIGISKPKGPKDIVRPLELSLIESVYLTKKGLINVVDKNGDLLEYKKLYEYSAMKINKFEILYKVYEDLREKGFIVRSGVKYGADFAVYTLGPGLEHAPYVVIAVDIDEEITPHELLSFGRVSHSTKKRLVLALVDRKSEGIRYIMFKWVKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="144" xmlns="https://uniprot.org/uniprot">
    <accession>Q980C0</accession>
    <name>Q980C0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ATP-dependent helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">SSO0394</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the Lhr helicase family. Lhr-Core subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90183">
        <property type="entry name" value="D90183"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988799.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980C0"/>
    <dbReference type="FunCoup" id="Q980C0">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0394"/>
    <dbReference type="PaxDb" id="273057-SSO0394"/>
    <dbReference type="EnsemblBacteria" id="AAK40723">
        <property type="protein sequence ID" value="AAK40723"/>
        <property type="gene ID" value="SSO0394"/>
    </dbReference>
    <dbReference type="GeneID" id="1455533"/>
    <dbReference type="KEGG" id="sso:SSO0394"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.390"/>
    <dbReference type="eggNOG" id="arCOG00557">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002025_0_0_2"/>
    <dbReference type="InParanoid" id="Q980C0"/>
    <dbReference type="PhylomeDB" id="Q980C0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052511">
        <property type="entry name" value="ATP-dep_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017170">
        <property type="entry name" value="Lhr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013701">
        <property type="entry name" value="Lhr-like_DEAD/DEAH_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045628">
        <property type="entry name" value="Lhr_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962:SF5">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08494">
        <property type="entry name" value="DEAD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19306">
        <property type="entry name" value="WHD_Lhr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037307">
        <property type="entry name" value="Lhr-like_helic_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="28"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="244"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK40723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="913" mass="104862" checksum="99B596770227FC15" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-31" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q980C1</accession>
    <name>RL14E_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L14e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl14e</name>
        <name type="ordered locus">SSO5763</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90183">
        <property type="entry name" value="C90183"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988801.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2JOY">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-96"/>
    </dbReference>
    <dbReference type="PDB" id="2KDS">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=2-96"/>
    </dbReference>
    <dbReference type="PDBsum" id="2JOY"/>
    <dbReference type="PDBsum" id="2KDS"/>
    <dbReference type="AlphaFoldDB" id="Q980C1"/>
    <dbReference type="BMRB" id="Q980C1"/>
    <dbReference type="SMR" id="Q980C1"/>
    <dbReference type="FunCoup" id="Q980C1">
        <property type="interactions" value="149"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO5763"/>
    <dbReference type="PaxDb" id="273057-SSO5763"/>
    <dbReference type="DNASU" id="1455532"/>
    <dbReference type="EnsemblBacteria" id="AAK40722">
        <property type="protein sequence ID" value="AAK40722"/>
        <property type="gene ID" value="SSO5763"/>
    </dbReference>
    <dbReference type="GeneID" id="1455532"/>
    <dbReference type="KEGG" id="sso:SSO5763"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.389"/>
    <dbReference type="eggNOG" id="arCOG04167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183474_0_0_2"/>
    <dbReference type="InParanoid" id="Q980C1"/>
    <dbReference type="PhylomeDB" id="Q980C1"/>
    <dbReference type="EvolutionaryTrace" id="Q980C1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23702">
        <property type="entry name" value="eL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000045">
        <property type="entry name" value="50S ribosomal protein L14e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00721">
        <property type="entry name" value="Ribosomal_eL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039660">
        <property type="entry name" value="Ribosomal_eL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023651">
        <property type="entry name" value="Ribosomal_eL14_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003320">
        <property type="entry name" value="PRK04333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000132056" description="Large ribosomal subunit protein eL14">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="3"/>
            <end position="5"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="8"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="13"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="21"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="29"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="33"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="39"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="47"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="55"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="82"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="89"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2JOY"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2KDS"/>
        </source>
    </evidence>
    <sequence length="96" mass="10861" checksum="CE935FA5F41CFB7F" modified="2001-10-01" version="1">MPAIEVGRICVKVKGREAGSKCVIVDIIDDNFVLVTGPKDITGVKRRRVNILHLEPTDKKIDIQKGASDEEVKKKLEESNLTEYMKEKIKIRMPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q980E2</accession>
    <name>Q980E2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA (cytosine(72)-C(5))-methyltransferase</fullName>
            <shortName evidence="5">tRNA:m(5)C72 MTase</shortName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sun-1</name>
        <name evidence="7" type="ordered locus">SSO0371</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in several tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>cytidine(72) in tRNA + S-adenosyl-L-methionine = 5-methylcytidine(72) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61988"/>
            <dbReference type="Rhea" id="RHEA-COMP:15996"/>
            <dbReference type="Rhea" id="RHEA-COMP:15997"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74483"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90180">
        <property type="entry name" value="F90180"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980E2"/>
    <dbReference type="SMR" id="Q980E2"/>
    <dbReference type="FunCoup" id="Q980E2">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0371"/>
    <dbReference type="PaxDb" id="273057-SSO0371"/>
    <dbReference type="EnsemblBacteria" id="AAK40701">
        <property type="protein sequence ID" value="AAK40701"/>
        <property type="gene ID" value="SSO0371"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0371"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.365"/>
    <dbReference type="eggNOG" id="arCOG00975">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_1_0_2"/>
    <dbReference type="InParanoid" id="Q980E2"/>
    <dbReference type="PhylomeDB" id="Q980E2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016428">
        <property type="term" value="F:tRNA (cytidine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07953">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02237">
        <property type="entry name" value="NSUN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043699">
        <property type="entry name" value="NSUN6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF34">
        <property type="entry name" value="TRNA (CYTOSINE(72)-C(5))-METHYLTRANSFERASE NSUN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="6">
        <location>
            <begin position="59"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="327" mass="37527" checksum="90637B8850AAE2E2" modified="2001-10-01" version="1">MNAIRKPNPRLYIRVNTLKTSVEKILERLPHFKPDEDFKEAIYVEIKGPNEINIHENKVIVDKKTAESAMLGANVYKPGLKSVVINNAKDKRVTVYSDNGIPVAEGKYYGMNTNLVVEVTNSLYTSPKLADLPELKNGLIYVQGKASMYVAHLLDPHPNEKIVDMTAYAGGKLTHIYQLEPRVKLIGFDHTEKKVEKLRDLANKMGMRIEIYKADSRYLYEDYKIGDVDKVVIDPPCSALGIRPKLYDHKTKQDILNFHKYQKQFLNSAYKILKKNGVVIYSTCTVTTWENEKVINDPRFSVEYEIRFHPHIHEITGFFIAKLIKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q980P6</accession>
    <name>Q980P6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP-grasp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">SSO0241</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90165">
        <property type="entry name" value="G90165"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990498.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980P6"/>
    <dbReference type="STRING" id="273057.SSO0241"/>
    <dbReference type="PaxDb" id="273057-SSO0241"/>
    <dbReference type="EnsemblBacteria" id="AAK40582">
        <property type="protein sequence ID" value="AAK40582"/>
        <property type="gene ID" value="SSO0241"/>
    </dbReference>
    <dbReference type="GeneID" id="1455392"/>
    <dbReference type="KEGG" id="sso:SSO0241"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.237"/>
    <dbReference type="eggNOG" id="arCOG04346">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065084_0_0_2"/>
    <dbReference type="InParanoid" id="Q980P6"/>
    <dbReference type="PhylomeDB" id="Q980P6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006188">
        <property type="term" value="P:IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023656">
        <property type="entry name" value="IMP_biosynth_PurP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009720">
        <property type="entry name" value="IMP_biosynth_PurP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010672">
        <property type="entry name" value="IMP_biosynth_PurP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147:SF1">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06849">
        <property type="entry name" value="DUF1246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06973">
        <property type="entry name" value="DUF1297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004602">
        <property type="entry name" value="ATPgrasp_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="11">
        <location>
            <begin position="101"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK40582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="349" mass="40019" checksum="4005C11304E6A054" modified="2001-10-01" version="1">MNVKIAALASHSALDVFDGAKDEGFQTIGLCKKGRERPYLEFKAIMDKCIILEDFKEIISDKVQSILLSENAIIVPNRSLAVYVGYDGIEKMKTKVFGNRYMLRWEERIGEKNYYKILNEAKISIPKLFKPEEIDRPVIVKLPEAKRKVERGFFFAVNKEDFEAKLNELLRNNVIDNEGLKNMVIEEFVFGAHFNLNYFYSPIFNRLELISVDRRIQSDWDSLYRLPADIQIKLGRIPKLIEVGHEPVTIRESLLEKVFEIGYRFVDATQKLEPPGIIGPFTLQVMVTPDLDLVVYDVAPRIGGGTNVYMGTGSQYSKFYFGKPISLGRRIALEIKHAIINNLTEKILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q980T5</accession>
    <name>Q980T5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutaredoxin related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90160">
        <property type="entry name" value="C90160"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990430.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980T5"/>
    <dbReference type="SMR" id="Q980T5"/>
    <dbReference type="STRING" id="273057.SSO0192"/>
    <dbReference type="PaxDb" id="273057-SSO0192"/>
    <dbReference type="EnsemblBacteria" id="AAK40538">
        <property type="protein sequence ID" value="AAK40538"/>
        <property type="gene ID" value="SSO0192"/>
    </dbReference>
    <dbReference type="GeneID" id="1455348"/>
    <dbReference type="KEGG" id="sso:SSO0192"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.191"/>
    <dbReference type="eggNOG" id="arCOG01218">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082677_0_0_2"/>
    <dbReference type="InParanoid" id="Q980T5"/>
    <dbReference type="PhylomeDB" id="Q980T5"/>
    <dbReference type="BRENDA" id="1.8.99.B1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="5.3.4.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02973">
        <property type="entry name" value="TRX_GRX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011903">
        <property type="entry name" value="TON_0319-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02187">
        <property type="entry name" value="PDO_seleno_TRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37170:SF1">
        <property type="entry name" value="GLUTAREDOXIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37170">
        <property type="entry name" value="GLUTAREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin-like fold" evidence="2">
        <location>
            <begin position="38"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin-like fold" evidence="2">
        <location>
            <begin position="150"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="233" mass="26033" checksum="017AECDBFCA898B5" modified="2001-10-01" version="1">MEQEYAELFSDEVKNALQDALKDMKNPVDVYVFVDNTDSNCQYCGVTTKFLEFMASAAPKDSNGSLLKVHIVDRANENSRKIFEEFNVERVPTVAFYNGYIRWTGAPLGEEIRALVETIVRLSQGESGLSQETVEAIKSKLNGKVKIETVVTPSCPYCPYAALMAHMVAFEACRAGKCNVISEVIEAYENQDIAEKYQVMSVPAIAINESIEFIGVPYEENFINAILEKQKIS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-02-12" modified="2025-06-18" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q980U8</accession>
    <name>FEN_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flap endonuclease 1</fullName>
            <shortName evidence="2">FEN-1</shortName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fen</name>
        <name type="ordered locus">SSO0179</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Mol. Cell" volume="11" first="275" last="282">
            <title>A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Dionne I."/>
                <person name="Nookala R.K."/>
                <person name="Jackson S.P."/>
                <person name="Doherty A.J."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="12535540"/>
            <dbReference type="DOI" id="10.1016/S1097-2765(02)00824-9"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>INTERACTION WITH PCNA1</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF 341-ARG--PHE-349</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2006" name="Nucleic Acids Res." volume="34" first="4515" last="4526">
            <title>Structure of an archaeal PCNA1-PCNA2-FEN1 complex: elucidating PCNA subunit and client enzyme specificity.</title>
            <authorList>
                <person name="Dore A.S."/>
                <person name="Kilkenny M.L."/>
                <person name="Jones S.A."/>
                <person name="Oliver A.W."/>
                <person name="Roe S.M."/>
                <person name="Bell S.D."/>
                <person name="Pearl L.H."/>
            </authorList>
            <dbReference type="PubMed" id="16945955"/>
            <dbReference type="DOI" id="10.1093/nar/gkl623"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 5-349 IN COMPLEX WITH PCNA</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA (By similarity). DNA polymerase I, DNA ligase and the flap endonuclease may be constitutively associated with the PCNA heterotrimer forming a scanning complex able to couple DNA synthesis and Okazaki fragment maturation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="3">Heterotrimeric PCNA stimulates the nuclease activity without altering cleavage specificity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 4">Interacts with PCNA via subunit PCNA1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40525" version="1"/>
        </conflict>
        <text>Truncated N-terminus.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40525.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="2IZO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="A=5-349"/>
    </dbReference>
    <dbReference type="PDBsum" id="2IZO"/>
    <dbReference type="AlphaFoldDB" id="Q980U8"/>
    <dbReference type="SMR" id="Q980U8"/>
    <dbReference type="FunCoup" id="Q980U8">
        <property type="interactions" value="199"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0179"/>
    <dbReference type="PaxDb" id="273057-SSO0179"/>
    <dbReference type="EnsemblBacteria" id="AAK40525">
        <property type="protein sequence ID" value="AAK40525"/>
        <property type="gene ID" value="SSO0179"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0179"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.176"/>
    <dbReference type="eggNOG" id="arCOG04050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032444_0_0_2"/>
    <dbReference type="InParanoid" id="Q980U8"/>
    <dbReference type="PhylomeDB" id="Q980U8"/>
    <dbReference type="BRENDA" id="3.1.99.B1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q980U8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09903">
        <property type="entry name" value="H3TH_FEN1-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000087">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000016">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019973">
        <property type="entry name" value="Flap_endonuc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03674">
        <property type="entry name" value="fen_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF9">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000154065" description="Flap endonuclease 1">
        <location>
            <begin position="1"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-domain">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="I-domain">
        <location>
            <begin position="116"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with PCNA">
        <location>
            <begin position="341"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="No interaction with PCNA1." evidence="3">
        <location>
            <begin position="341"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="16"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="22"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="50"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="73"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="146"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="165"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="172"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="175"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="182"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="208"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="212"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="223"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="236"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="281"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="304"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="311"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="345"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12535540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="16945955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="2IZO"/>
        </source>
    </evidence>
    <sequence length="349" mass="39598" checksum="7210395FD749C94C" modified="2003-02-12" version="2">MDLADLVKDVKRELSFSELKGKRVSIDGYNALYQFLAAIRQPDGTPLMDSQGRVTSHLSGLFYRTINILEEGVIPIYVFDGKPPEQKSEELERRRKAKEEAERKLERAKSEGKIEELRKYSQAILRLSNIMVEESKKLLRAMGIPIVQAPSEGEAEAAYLNKLGLSWAAASQDYDAILFGAKRLVRNLTITGKRKLPNKDVYVEIKPELIETEILLKKLGITREQLIDIGILIGTDYNPDGIRGIGPERALKIIKKYGKIEKAMEYGEISKKDINFNIDEIRGLFLNPQVVKPEEALDLNEPNGEDIINILVYEHNFSEERVKNGIERLTKAIKEAKGASRQTGLDRWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Y7</accession>
    <name>Q980Y7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposase IS4-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90153">
        <property type="entry name" value="F90153"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Y7"/>
    <dbReference type="PaxDb" id="273057-SSO0132"/>
    <dbReference type="DNASU" id="1453312"/>
    <dbReference type="EnsemblBacteria" id="AAK40485">
        <property type="protein sequence ID" value="AAK40485"/>
        <property type="gene ID" value="SSO0132"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0132"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096713_2_0_2"/>
    <dbReference type="InParanoid" id="Q980Y7"/>
    <dbReference type="PhylomeDB" id="Q980Y7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="15287" checksum="16750632CEEFE400" modified="2001-10-01" version="1">MGDLPPGSYLADLIMGDHTITVKLLVLEYKDSRLNFYTTDLNMEDEMIEVTWKIRWEIEKLHRDVKALDMQDSSFLKRQRFHGYLLLFVMVVNAVRDLIGSLKLKSVEELLKFIENHLGGAPGLMKMFKLR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-08-29" modified="2026-01-28" version="137" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Z8</accession>
    <name>RPO10_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo10</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo10</name>
        <name evidence="1 3" type="synonym">rpoN</name>
        <name type="ordered locus">SSO5140</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2008" name="Nature" volume="451" first="851" last="854">
            <title>The X-ray crystal structure of RNA polymerase from Archaea.</title>
            <authorList>
                <person name="Hirata A."/>
                <person name="Klein B.J."/>
                <person name="Murakami K.S."/>
            </authorList>
            <dbReference type="PubMed" id="18235446"/>
            <dbReference type="DOI" id="10.1038/nature06530"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF THE RNA POLYMERASE COMPLEX IN COMPLEX WITH ZINC</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2 4 5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1 2 4 5">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 13-subunit RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90146">
        <property type="entry name" value="F90146"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988875.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2PMZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="N/Y=1-66"/>
    </dbReference>
    <dbReference type="PDB" id="3HKZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="N/W=1-66"/>
    </dbReference>
    <dbReference type="PDBsum" id="2PMZ"/>
    <dbReference type="PDBsum" id="3HKZ"/>
    <dbReference type="AlphaFoldDB" id="Q980Z8"/>
    <dbReference type="SMR" id="Q980Z8"/>
    <dbReference type="DIP" id="DIP-60643N"/>
    <dbReference type="FunCoup" id="Q980Z8">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="IntAct" id="Q980Z8">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO5140"/>
    <dbReference type="PaxDb" id="273057-SSO5140"/>
    <dbReference type="EnsemblBacteria" id="AAK40429">
        <property type="protein sequence ID" value="AAK40429"/>
        <property type="gene ID" value="SSO5140"/>
    </dbReference>
    <dbReference type="GeneID" id="1455320"/>
    <dbReference type="KEGG" id="sso:SSO5140"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.68"/>
    <dbReference type="eggNOG" id="arCOG04244">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143122_1_1_2"/>
    <dbReference type="InParanoid" id="Q980Z8"/>
    <dbReference type="PhylomeDB" id="Q980Z8"/>
    <dbReference type="BRENDA" id="2.7.7.6">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q980Z8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000335">
        <property type="entry name" value="DNA-directed RNA polymerase subunit N, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003089">
        <property type="entry name" value="PRK04016.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005653">
        <property type="entry name" value="RNA_pol_N/8_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="RNA polymerase subunit RPB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000121362" description="DNA-directed RNA polymerase subunit Rpo10">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 4 5">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 4 5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 4 5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 4 5">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="8"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="15"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="21"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="31"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="37"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="44"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="56"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18235446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="11427726"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="4">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="5">
        <source>
            <dbReference type="PDB" id="3HKZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <sequence length="66" mass="7591" checksum="C6774B541A1CFA13" modified="2001-08-29" version="1">MLIPIRCFTCGSLIADKWQSFITRVNAGENPGKVLDDLGVKRYCCRRMLLSHVDIINEVIHYTRPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q981A7</accession>
    <name>Q981A7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1234</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90143">
        <property type="entry name" value="H90143"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981A7"/>
    <dbReference type="PaxDb" id="273057-SSO0042"/>
    <dbReference type="EnsemblBacteria" id="AAK40407">
        <property type="protein sequence ID" value="AAK40407"/>
        <property type="gene ID" value="SSO0042"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0042"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.43"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075479_0_0_2"/>
    <dbReference type="InParanoid" id="Q981A7"/>
    <dbReference type="PhylomeDB" id="Q981A7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="217" mass="25071" checksum="8BA3D532B0F5B324" modified="2001-10-01" version="1">MGRLYGVRKKLLEISFKLEEKCLPSYLPASAHLVDFMAWLVDSFLLDLPPGKRSVETFREKAELERREGNLERAGKLLSLGRTKRRFEGRWTKKRGVSHYGLKAVAVISVSLFMRSITVKPANFSDKRFKSPLKGIKIADRGFSPSPTQLIAREKPFTTLRAHVEFFGTYLNAFWRPYGTTTWRNDVFLHVLGVIYNIKIFLAIQRRTPPERRAVQL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-08" modified="2026-01-28" version="141" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UWW2</accession>
    <name>SYM_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Methionyl-tRNA synthetase</fullName>
            <shortName evidence="1">MetRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">metG</name>
        <name type="synonym">metS</name>
        <name type="synonym">symC</name>
        <name type="ordered locus">SSO0558</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of L-methionine to tRNA(Met). Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:13482"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90202">
        <property type="entry name" value="D90202"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991068.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UWW2"/>
    <dbReference type="SMR" id="Q9UWW2"/>
    <dbReference type="FunCoup" id="Q9UWW2">
        <property type="interactions" value="354"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0558"/>
    <dbReference type="PaxDb" id="273057-SSO0558"/>
    <dbReference type="EnsemblBacteria" id="AAK40875">
        <property type="protein sequence ID" value="AAK40875"/>
        <property type="gene ID" value="SSO0558"/>
    </dbReference>
    <dbReference type="GeneID" id="1454842"/>
    <dbReference type="GeneID" id="44129564"/>
    <dbReference type="KEGG" id="sso:SSO0558"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.568"/>
    <dbReference type="eggNOG" id="arCOG00810">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_1_2_2"/>
    <dbReference type="InParanoid" id="Q9UWW2"/>
    <dbReference type="PhylomeDB" id="Q9UWW2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.20:FF:000001">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="entry name" value="Methionyl-tRNA synthetase, Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="Methionyl-tRNA synthetase (MetRS), Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000139200" description="Methionine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="1">
        <location>
            <begin position="333"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00098"/>
        </source>
    </evidence>
    <sequence length="573" mass="66517" checksum="5116B2EECA58ACEE" modified="2000-05-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX91</accession>
    <name>RL6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl6</name>
        <name evidence="1" type="synonym">rpl6Ab</name>
        <name type="ordered locus">SSO0702</name>
        <name type="ORF">C10_028</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90218">
        <property type="entry name" value="D90218"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991260.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX91"/>
    <dbReference type="SMR" id="Q9UX91"/>
    <dbReference type="FunCoup" id="Q9UX91">
        <property type="interactions" value="202"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0702"/>
    <dbReference type="PaxDb" id="273057-SSO0702"/>
    <dbReference type="EnsemblBacteria" id="AAK41003">
        <property type="protein sequence ID" value="AAK41003"/>
        <property type="gene ID" value="SSO0702"/>
    </dbReference>
    <dbReference type="GeneID" id="1454970"/>
    <dbReference type="KEGG" id="sso:SSO0702"/>
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            <fullName evidence="1">Exosome complex component Rrp41</fullName>
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            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
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    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
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            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="EMBO Rep." volume="4" first="889" last="893">
            <title>An exosome-like complex in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Evguenieva-Hackenberg E."/>
                <person name="Walter P."/>
                <person name="Hochleitner E."/>
                <person name="Lottspeich F."/>
                <person name="Klug G."/>
            </authorList>
            <dbReference type="PubMed" id="12947419"/>
            <dbReference type="DOI" id="10.1038/sj.embor.embor929"/>
        </citation>
        <scope>INTERACTION WITH EXOSOME</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2006" name="Mol. Microbiol." volume="62" first="1076" last="1089">
            <title>Characterization of native and reconstituted exosome complexes from the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Walter P."/>
                <person name="Klein F."/>
                <person name="Lorentzen E."/>
                <person name="Ilchmann A."/>
                <person name="Klug G."/>
                <person name="Evguenieva-Hackenberg E."/>
            </authorList>
            <dbReference type="PubMed" id="17078816"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.2006.05393.x"/>
        </citation>
        <scope>INTERACTION WITH EXOSOME</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2010" name="FEBS Lett." volume="584" first="2931" last="2936">
            <title>The evolutionarily conserved subunits Rrp4 and Csl4 confer different substrate specificities to the archaeal exosome.</title>
            <authorList>
                <person name="Roppelt V."/>
                <person name="Klug G."/>
                <person name="Evguenieva-Hackenberg E."/>
            </authorList>
            <dbReference type="PubMed" id="20488184"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2010.05.014"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2012" name="Biochimie" volume="94" first="1578" last="1587">
            <title>Heterogeneous complexes of the RNA exosome in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Witharana C."/>
                <person name="Roppelt V."/>
                <person name="Lochnit G."/>
                <person name="Klug G."/>
                <person name="Evguenieva-Hackenberg E."/>
            </authorList>
            <dbReference type="PubMed" id="22503705"/>
            <dbReference type="DOI" id="10.1016/j.biochi.2012.03.026"/>
        </citation>
        <scope>INTERACTION WITH EXOSOME</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2005" name="Mol. Cell" volume="20" first="473" last="481">
            <title>Structural basis of 3' end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core.</title>
            <authorList>
                <person name="Lorentzen E."/>
                <person name="Conti E."/>
            </authorList>
            <dbReference type="PubMed" id="16285928"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2005.10.020"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) IN COMPLEX WITH RRP42</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF ARG-98 AND ARG-99</scope>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2005" name="Nat. Struct. Mol. Biol." volume="12" first="575" last="581">
            <title>The archaeal exosome core is a hexameric ring structure with three catalytic subunits.</title>
            <authorList>
                <person name="Lorentzen E."/>
                <person name="Walter P."/>
                <person name="Fribourg S."/>
                <person name="Evguenieva-Hackenberg E."/>
                <person name="Klug G."/>
                <person name="Conti E."/>
            </authorList>
            <dbReference type="PubMed" id="15951817"/>
            <dbReference type="DOI" id="10.1038/nsmb952"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) IN COMPLEX WITH RRP42</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF ASP-182</scope>
    </reference>
    <reference key="9">
        <citation type="journal article" date="2007" name="EMBO Rep." volume="8" first="470" last="476">
            <title>RNA channelling by the archaeal exosome.</title>
            <authorList>
                <person name="Lorentzen E."/>
                <person name="Dziembowski A."/>
                <person name="Lindner D."/>
                <person name="Seraphin B."/>
                <person name="Conti E."/>
            </authorList>
            <dbReference type="PubMed" id="17380186"/>
            <dbReference type="DOI" id="10.1038/sj.embor.7400945"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH RRP42 AND RRP4</scope>
        <scope>FUNCTION</scope>
        <scope>RNA-BINDING</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="10">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E8739" last="E8739">
            <title>Crystal structure of the S. solfataricus archaeal exosome reveals conformational flexibility in the RNA-binding ring.</title>
            <authorList>
                <person name="Lu C."/>
                <person name="Ding F."/>
                <person name="Ke A."/>
            </authorList>
            <dbReference type="PubMed" id="20090900"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0008739"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.41 ANGSTROMS) OF 4-248 IN COMPLEX WITH RRP42 AND RRP4</scope>
    </reference>
    <reference key="11">
        <citation type="journal article" date="2012" name="Archaea" volume="2012" first="721869" last="721869">
            <title>Crystal structure of a 9-subunit archaeal exosome in pre-catalytic states of the phosphorolytic reaction.</title>
            <authorList>
                <person name="Lorentzen E."/>
                <person name="Conti E."/>
            </authorList>
            <dbReference type="PubMed" id="23319881"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF MUTANT ALA-182 IN COMPLEX WITH RRP42 AND RRP4</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 4 6">Catalytic component of the exosome, which is a complex involved in RNA degradation. Has 3'-&gt;5' exoribonuclease activity. Can also synthesize heteromeric RNA-tails. Binds RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3 4 5 6 7">Component of the archaeal exosome complex. Forms a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. The hexameric ring associates with a trimer of Rrp4 and/or Csl4 subunits.</text>
    </comment>
    <comment type="interaction">
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            <id>Q9UXC2</id>
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        <interactant intactId="EBI-9009550">
            <id>Q9UXC0</id>
            <label>rrp42</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>3</experiments>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase PH family. Rrp41 subfamily.</text>
    </comment>
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        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11366">
        <property type="entry name" value="RNase_PH_archRRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000004">
        <property type="entry name" value="Exosome complex component Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00591">
        <property type="entry name" value="Exosome_Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011807">
        <property type="entry name" value="Rrp41"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02065">
        <property type="entry name" value="ECX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF0">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0271">Exosome</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000139991" description="Exosome complex component Rrp41">
        <location>
            <begin position="1"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abolishes exoribonuclease activity; when associated with E-99." evidence="3">
        <original>R</original>
        <variation>E</variation>
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abolishes exoribonuclease activity; when associated with E-98." evidence="3">
        <original>R</original>
        <variation>E</variation>
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abolishes both exoribonuclease and polyadenylation activities." evidence="2">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="2"/>
            <end position="4"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="15"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="31"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="67"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="72"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="76"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="87"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="117"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="138"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="157"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="162"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="172"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="179"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="195"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="199"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="211"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="238"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15951817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="16285928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="17380186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="20090900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="20488184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="23319881"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="2C39"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="2JE6"/>
        </source>
    </evidence>
    <sequence length="248" mass="27578" checksum="EAB2C2C89DD5854C" modified="2000-05-01" version="1">MREMLQVERPKLILDDGKRTDGRKPDELRSIKIELGVLKNADGSAIFEMGNTKAIAAVYGPKEMHPRHLSLPDRAVLRVRYHMTPFSTDERKNPAPSRREIELSKVIREALESAVLVELFPRTAIDVFTEILQADAGSRLVSLMAASLALADAGIPMRDLIAGVAVGKADGVIILDLNETEDMWGEADMPIAMMPSLNQVTLFQLNGSMTPDEFRQAFDLAVKGINIIYNLEREALKSKYVEFKEEGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-04-27" modified="2025-06-18" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>P95958</accession>
    <name>Y103_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein SSO0103</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0103</name>
        <name type="ORF">C04019</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CinA family.</text>
    </comment>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75386">
        <property type="entry name" value="S75386"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95958"/>
    <dbReference type="SMR" id="P95958"/>
    <dbReference type="FunCoup" id="P95958">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0103"/>
    <dbReference type="PaxDb" id="273057-SSO0103"/>
    <dbReference type="EnsemblBacteria" id="AAK40459">
        <property type="protein sequence ID" value="AAK40459"/>
        <property type="gene ID" value="SSO0103"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0103"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.100"/>
    <dbReference type="eggNOG" id="arCOG00215">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030805_0_5_2"/>
    <dbReference type="InParanoid" id="P95958"/>
    <dbReference type="PhylomeDB" id="P95958"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00885">
        <property type="entry name" value="cinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00226_A">
        <property type="entry name" value="CinA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050101">
        <property type="entry name" value="CinA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023055">
        <property type="entry name" value="CinA_Arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002291">
        <property type="entry name" value="PRK01215.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13939">
        <property type="entry name" value="NICOTINAMIDE-NUCLEOTIDE AMIDOHYDROLASE PNCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13939:SF0">
        <property type="entry name" value="NMN AMIDOHYDROLASE-LIKE PROTEIN YFAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156802" description="Protein SSO0103">
        <location>
            <begin position="1"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00226"/>
        </source>
    </evidence>
    <sequence length="272" mass="30906" checksum="366C0343A8E4EB3A" modified="1997-05-01" version="1">MVSIVNIFMDYWFAEIITIGNEVLSGKTVNTNASHIGRRLTSLGFTVRRITAVMDEVDEIASAFREAIDRKPRVIVSSGGLGPTWDDKTAEGLAKALGVNLELNKTAFDMILEKYMTRKIPITEERKKMAYMPYGAIPVENNEGIAPGIYVYHNNIDILATPGVPREMENVLENFINKMLRNRSNLKYLEDFIYVENVMESSLAPYVKELVKKYDIYIKTHPKSYEMSHPILEIQIAGSGKQEEEIKVKIEKVKFELLDAIKKLNGIIRNSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>P96038</accession>
    <name>RL1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Large ribosomal subunit protein uL1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpl1</name>
        <name evidence="2" type="synonym">rpl1Ab</name>
        <name type="ordered locus">SSO0345</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="Biochim. Biophys. Acta" volume="1340" first="170" last="177">
            <title>Nucleotide sequence of a gene cluster encoding NusG and the L11-L1-L10-L12 ribosomal proteins from the thermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Geiger M."/>
                <person name="Groebner P."/>
                <person name="Piendl W."/>
            </authorList>
            <dbReference type="PubMed" id="9252104"/>
            <dbReference type="DOI" id="10.1016/S0167-4838(97)00073-3"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1998" name="Eur. J. Biochem." volume="256" first="97" last="105">
            <title>Interaction of ribosomal L1 proteins from mesophilic and thermophilic Archaea and Bacteria with specific L1-binding sites on 23S rRNA and mRNA.</title>
            <authorList>
                <person name="Koehrer C."/>
                <person name="Mayer C."/>
                <person name="Neumair O."/>
                <person name="Groebner P."/>
                <person name="Piendl W."/>
            </authorList>
            <dbReference type="PubMed" id="9746351"/>
            <dbReference type="DOI" id="10.1046/j.1432-1327.1998.2560097.x"/>
        </citation>
        <scope>BINDING TO METHANOCOCCUS VANNIELII 23S RRNA AND TO M.VANNIELII L1 MRNA</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">Probably involved in E site tRNA release (By similarity). Binds directly to 23S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Protein L1 is also a translational repressor protein, it controls the translation of its operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text>Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="U85262">
        <property type="protein sequence ID" value="AAB99525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90177">
        <property type="entry name" value="C90177"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990633.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P96038"/>
    <dbReference type="SMR" id="P96038"/>
    <dbReference type="FunCoup" id="P96038">
        <property type="interactions" value="179"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0345"/>
    <dbReference type="PaxDb" id="273057-SSO0345"/>
    <dbReference type="EnsemblBacteria" id="AAK40674">
        <property type="protein sequence ID" value="AAK40674"/>
        <property type="gene ID" value="SSO0345"/>
    </dbReference>
    <dbReference type="GeneID" id="1455484"/>
    <dbReference type="KEGG" id="sso:SSO0345"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.335"/>
    <dbReference type="eggNOG" id="arCOG04289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062853_4_0_2"/>
    <dbReference type="InParanoid" id="P96038"/>
    <dbReference type="PhylomeDB" id="P96038"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000005">
        <property type="entry name" value="50S ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_A">
        <property type="entry name" value="Ribosomal_uL1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023669">
        <property type="entry name" value="Ribosomal_uL1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_uL1_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003244">
        <property type="entry name" value="PRK04203.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000125813" description="Large ribosomal subunit protein uL1">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="218" mass="24527" checksum="A74D93D2EBA80C3A" modified="1997-05-01" version="1">MQIVDRSNLEASLKLALSPENNPKRNFVQSVEIIVTFKEVDMKKGDLKLREIVVLPKPPEKTKKVLVVPTIQQLEYAKKAEPNTILTKEELQKLQGNKRAIKKIARQNDWFLIAPDSMALVGRILGPALGPRGKFPTPLPNTADISEYILRFKRSTLVKTKDQPQTQVFIGTESQQIADLAENALAVLNVIESKGYAQKVRNIYVKTTMGKVVKVELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-01-24" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX76</accession>
    <name>Q7LX76_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74032">
        <property type="entry name" value="S74032"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923777.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX76"/>
    <dbReference type="PaxDb" id="273057-SSO2120"/>
    <dbReference type="EnsemblBacteria" id="AAK42299">
        <property type="protein sequence ID" value="AAK42299"/>
        <property type="gene ID" value="SSO2120"/>
    </dbReference>
    <dbReference type="GeneID" id="1453626"/>
    <dbReference type="KEGG" id="sso:SSO2120"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2210"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX76"/>
    <dbReference type="PhylomeDB" id="Q7LX76"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="80"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37372" checksum="C0386D9F182C3915" modified="2011-05-31" version="1">MQTMILPKTELKSLAITLATNNINVISQDLDPEIVKAAPSLLTGNRGKYYLKVVRQGEKVISKGQRTFKFYPIYREVKGETNVVAVDETGLTVGEKEQEKAEGFLLYNWKRKGIKMRSLDLVYPLRLPLLVEVADLRSDSPSQFLLRSVREVSQYMEIDYVVADAGFLNLGVIKEMPVKTIVRGKSNLKGFKELSNVPLVEKRYEVKDKVYVAYRVLEFEGLYYYDVVYVKGKPRHFMFVTNFEGDPYELAELYRLRWQVEEGFKVRKARIRYVRKLSNKIFLFLYYTVLDSAWNLVNHLLFNFKSTCKKVLSFDSFVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX78</accession>
    <name>Q7LX78_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF973 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74030">
        <property type="entry name" value="S74030"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990151.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX78"/>
    <dbReference type="PaxDb" id="273057-SSO2118"/>
    <dbReference type="EnsemblBacteria" id="AAK42297">
        <property type="protein sequence ID" value="AAK42297"/>
        <property type="gene ID" value="SSO2118"/>
    </dbReference>
    <dbReference type="GeneID" id="1453624"/>
    <dbReference type="KEGG" id="sso:SSO2118"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2207"/>
    <dbReference type="eggNOG" id="arCOG03768">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_876089_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX78"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009321">
        <property type="entry name" value="DUF973"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06157">
        <property type="entry name" value="DUF973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="33966" checksum="C6970B8A17DC1991" modified="2011-05-31" version="1">MSQQNREILGLQEVRNGVKYLLISEIFGIIFEIIVIATLFAINNLDIGLSVGIFGLVIQILLLYLTYVNIKSGFQILTSLGRDLGRGITSIILIVAGTAITIIAFIFLLPVLFSAQALNPSVAVGTILSSAVGAVAFALIGGVLGLVGYILLFLAFMKTGEIYNNKDIKDGGLIALIGFILSIIISIIGLIITIIGFYKIYSGLGNIIGNLPSQQQQLPTMFQPSLPIEQVGVGKLYSNGIAEATIYSQFQLGILTATILGTSYSTSNIVPNQLSVGYNNIKIDFKASFTFIAGNIYVVQLMLSNGQTLNISVTYQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXD9</accession>
    <name>Q7LXD9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase ISC1476</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO0842</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90234">
        <property type="entry name" value="G90234"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923129.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXD9"/>
    <dbReference type="SMR" id="Q7LXD9"/>
    <dbReference type="PaxDb" id="273057-SSO0842"/>
    <dbReference type="EnsemblBacteria" id="AAK41134">
        <property type="protein sequence ID" value="AAK41134"/>
        <property type="gene ID" value="SSO0842"/>
    </dbReference>
    <dbReference type="GeneID" id="1455101"/>
    <dbReference type="KEGG" id="sso:SSO0842"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.854"/>
    <dbReference type="eggNOG" id="arCOG00683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040997_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LXD9"/>
    <dbReference type="PhylomeDB" id="Q7LXD9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF21">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="230"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="364"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="460" mass="53519" checksum="0A76FB1D1D487840" modified="2011-05-31" version="1">MPSSGQLYGDEERELTPTPAIPEEGVYEVKYSNRRTNVVRLLPNGFQERKLRRLANLSAKLFNEVNFERRQQFFHEGKVNIEETYKKYYEKYKVILGVNAQAVLNKNNEAWSSFFSLLKLKKEGKLPPHMNHVSPPGYWKDREAGKRKQILVIRQDRYKVDAENHKLILKDFDMEIDFIGRLKWYGKQGRLEIIFDETRNAWYAHIPIEVGVEETKAGKKSKHVVKGERKSIQISKPKGNKVASIDLGINVIASVVVNDGTWLLYKGVRTKEDYFYFQKRIAEVQSLADRTRNIGEYDAYLELLREKRRLFKKLTRRLTHLYRNLASHLIETLYELGVSTIYLGYPFNIVQEKGNKFTVNMWSYRKLMESIELKAQEYGMKVFEVVEYNTSKYCAYHDVEVKRNPRGVINCPLGHKLHSDLNGALNILKKALGVIVNAVKKPLSFIVDHNRVAPIKGCNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2023-11-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXJ0</accession>
    <name>Q7LXJ0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4898 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0783</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90228">
        <property type="entry name" value="B90228"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXJ0"/>
    <dbReference type="PaxDb" id="273057-SSO0783"/>
    <dbReference type="EnsemblBacteria" id="AAK41081">
        <property type="protein sequence ID" value="AAK41081"/>
        <property type="gene ID" value="SSO0783"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0783"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.786"/>
    <dbReference type="eggNOG" id="arCOG08428">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2091409_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXJ0"/>
    <dbReference type="PhylomeDB" id="Q7LXJ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032603">
        <property type="entry name" value="DUF4898"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16239">
        <property type="entry name" value="DUF4898"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="15472" checksum="80722C2800B46087" modified="2011-05-31" version="1">MQQWNYTIKEFIINLINVFTHDIYYMKSLSPKELETYVDEDLLRVINREQINFIYFINARIISSYEKFFRFFIPDSTKYLVLVSSDLHLKFIKESVIRAKEPLEVSCYVSSKLPPMSFLIIVVEKKEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXM1</accession>
    <name>Q7LXM1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF106 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90217">
        <property type="entry name" value="C90217"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991246.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXM1"/>
    <dbReference type="STRING" id="273057.SSO0693"/>
    <dbReference type="PaxDb" id="273057-SSO0693"/>
    <dbReference type="EnsemblBacteria" id="AAK40994">
        <property type="protein sequence ID" value="AAK40994"/>
        <property type="gene ID" value="SSO0693"/>
    </dbReference>
    <dbReference type="GeneID" id="71027412"/>
    <dbReference type="KEGG" id="sso:SSO0693"/>
    <dbReference type="eggNOG" id="arCOG07287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1763763_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXM1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17795" checksum="0C2EA4692DE0DDEB" modified="2011-05-31" version="1">MELNTIYIFLISLFSNFVVYLIYKYYFYGKIINKISLVEKYEERLKNIASSKRREKAYKKISKELESYISSVRYYMFLRSMILVGVYIVDLVIILNYLNTNIYLPFYIPYLTAKSSSNMYLMLNGSLFEFIASYILFTPLSLRSNLKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXV1</accession>
    <name>Q7LXV1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90201">
        <property type="entry name" value="C90201"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923029.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXV1"/>
    <dbReference type="STRING" id="273057.SSO0549"/>
    <dbReference type="PaxDb" id="273057-SSO0549"/>
    <dbReference type="EnsemblBacteria" id="AAK40866">
        <property type="protein sequence ID" value="AAK40866"/>
        <property type="gene ID" value="SSO0549"/>
    </dbReference>
    <dbReference type="GeneID" id="1454833"/>
    <dbReference type="KEGG" id="sso:SSO0549"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.557"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXV1"/>
    <dbReference type="PhylomeDB" id="Q7LXV1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:DNA transposition"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Transposase_11"/>
    </dbReference>
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        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="80"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37425" checksum="0B54CE958A1E9861" modified="2011-05-31" version="1">MQTMILPKTELKSLAITLATNNINVISQDLDPEIVKAAPSLLTGNRGKYYLKVVRRGEKVISKGQRTFKFYPIYREVKGEINVVAVDETGLTVGEKEQEKAEGFLLYNWKRKGVKMRSLDLVYPLRLPLLVEVADLRSDSPSQFLLRSVREVSQYMEIDYVVADAGFLNLGVIKEMPVKTIVRGKSNLKGFKELSNVPLVEKRYEVKDRVYVAYRVLKFEGLYYYDVVYVKGKPRHFMFVTNFEGDPYELAELYRLRWQVEEGFKVRKARIRYVRKLSNKIFLFLYYTVLDSAWNLVNHLLFNFKSTCKKVLSFDSFVKLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2025-06-18" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TX6</accession>
    <name>TRPB2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Tryptophan synthase beta chain 2</fullName>
            <ecNumber>4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">trpB2</name>
        <name type="ordered locus">SSO1145</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90267">
        <property type="entry name" value="E90267"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923209.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="4QYS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.94 A"/>
        <property type="chains" value="A/B=1-429"/>
    </dbReference>
    <dbReference type="PDB" id="6HTE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.96 A"/>
        <property type="chains" value="B/D=4-428"/>
    </dbReference>
    <dbReference type="PDBsum" id="4QYS"/>
    <dbReference type="PDBsum" id="6HTE"/>
    <dbReference type="AlphaFoldDB" id="Q97TX6"/>
    <dbReference type="SMR" id="Q97TX6"/>
    <dbReference type="FunCoup" id="Q97TX6">
        <property type="interactions" value="206"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1145"/>
    <dbReference type="PaxDb" id="273057-SSO1145"/>
    <dbReference type="EnsemblBacteria" id="AAK41396">
        <property type="protein sequence ID" value="AAK41396"/>
        <property type="gene ID" value="SSO1145"/>
    </dbReference>
    <dbReference type="GeneID" id="1454175"/>
    <dbReference type="KEGG" id="sso:SSO1145"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1136"/>
    <dbReference type="eggNOG" id="arCOG01432">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042858_1_0_2"/>
    <dbReference type="InParanoid" id="Q97TX6"/>
    <dbReference type="PhylomeDB" id="Q97TX6"/>
    <dbReference type="BRENDA" id="4.2.1.20">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="STRENDA-DB" id="LQZ1C3">
        <property type="experiment" value="steady-state kinetics with TrpB2o from Thermotoga maritima"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97TX6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052684">
        <property type="term" value="F:L-serine hydro-lyase (adding indole, L-tryptophan-forming) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
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    </dbReference>
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        <property type="entry name" value="Trp_synth_b-like"/>
    </dbReference>
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        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
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        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009057">
        <property type="entry name" value="PRK12391.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="trpB_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF6">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500824">
        <property type="entry name" value="TrpB_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000099056" description="Tryptophan synthase beta chain 2">
        <location>
            <begin position="1"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
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            <begin position="11"/>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
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        </location>
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    </feature>
    <feature type="strand" evidence="3">
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        </location>
    </feature>
    <feature type="turn" evidence="3">
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            <begin position="37"/>
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        </location>
    </feature>
    <feature type="turn" evidence="3">
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        </location>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
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        </location>
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        </location>
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        </location>
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        </location>
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        </location>
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    </feature>
    <feature type="helix" evidence="3">
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        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="4QYS"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U42</accession>
    <name>Q97U42_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thermopsin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99503">
        <property type="entry name" value="A99503"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991710.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U42"/>
    <dbReference type="STRING" id="273057.SSO3181"/>
    <dbReference type="PaxDb" id="273057-SSO3181"/>
    <dbReference type="EnsemblBacteria" id="AAK43280">
        <property type="protein sequence ID" value="AAK43280"/>
        <property type="gene ID" value="SSO3181"/>
    </dbReference>
    <dbReference type="GeneID" id="1453199"/>
    <dbReference type="KEGG" id="sso:SSO3181"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3285"/>
    <dbReference type="eggNOG" id="arCOG07853">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_714996_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U42"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="43847" checksum="3DCF852986329D83" modified="2001-10-01" version="1">MRRLSLLTLTLLFLTPLMSHGNVVIVNYSSYQIHGSEILYSYSNSSYLIQEKLTPINITSVNYILQISYFPNWTLDISTNLPNIFSIFVGNYLIWSGFETNGLTASFLIPYMNPLNLTIWVMNYPNPIPLNTIIPMGEEVVLHNGEISNLYIFKTEILKTIKLNTTLPSFFSYPSYYILTYNYTYAPVTVFNSSEGQIDIIRGINGITVLEVGLVHFNNLTFYVYNISNQRVYPAVEIVKNLVITTKGVETPFIALSSLIEDNLSLVIVNSTAALIIVNKSITYMPIHDVTIEEPSFIFMQGKFFLGYSIFVNNPGIYRFSGHYNLFNAPYVTTKGSTYFITFDKGYYFALPRSLSLSINPIPFLFAAIISSLLTILILLINKIFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UA6</accession>
    <name>Q97UA6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ChsH2 rubredoxin-like zinc ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90494">
        <property type="entry name" value="H90494"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990935.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UA6"/>
    <dbReference type="SMR" id="Q97UA6"/>
    <dbReference type="STRING" id="273057.SSO3112"/>
    <dbReference type="PaxDb" id="273057-SSO3112"/>
    <dbReference type="EnsemblBacteria" id="AAK43215">
        <property type="protein sequence ID" value="AAK43215"/>
        <property type="gene ID" value="SSO3112"/>
    </dbReference>
    <dbReference type="GeneID" id="7808212"/>
    <dbReference type="KEGG" id="sso:SSO3112"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3219"/>
    <dbReference type="eggNOG" id="arCOG01289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119412_1_2_2"/>
    <dbReference type="InParanoid" id="Q97UA6"/>
    <dbReference type="PhylomeDB" id="Q97UA6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="ChsH2_Znr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF5">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="1">
        <location>
            <begin position="14"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="116" mass="13539" checksum="F86B3FC0F23BE28A" modified="2001-10-01" version="1">MRLEEIYSTYDKIVQSGYLPYIRCRKCMNTFFYVRNLCPKCGSRDLEVLRSSGIGKVFSWTKIFRKNDTSIYGIVELEEGFKIYCNFASDVEIGDKVKVKINSLEDGKYKVLAYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UE5</accession>
    <name>Q97UE5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90490">
        <property type="entry name" value="A90490"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UE5"/>
    <dbReference type="STRING" id="273057.SSO3077"/>
    <dbReference type="PaxDb" id="273057-SSO3077"/>
    <dbReference type="EnsemblBacteria" id="AAK43176">
        <property type="protein sequence ID" value="AAK43176"/>
        <property type="gene ID" value="SSO3077"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3077"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1912433_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UE5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="132" mass="14911" checksum="0C891F4FF426E241" modified="2001-10-01" version="1">MEELERLEARKIEDRLGELVPKDSLIFTIPGIDEILGCIILARAFSNKPFGQTSSIVSTKVATWVLSTHVAIRGRAYLFRLIRPIIIPNTPNNPCQLPSQSSVQLIFVNFITMFSPTVGVSHASRRRFHFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UF9</accession>
    <name>Q97UF9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inosine/uridine-preferring nucleoside hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO11870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90488">
        <property type="entry name" value="C90488"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UF9"/>
    <dbReference type="PaxDb" id="273057-SSO11870"/>
    <dbReference type="EnsemblBacteria" id="AAK43162">
        <property type="protein sequence ID" value="AAK43162"/>
        <property type="gene ID" value="SSO11870"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11870"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3160"/>
    <dbReference type="eggNOG" id="arCOG04558">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164504_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UF9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="87" mass="9777" checksum="9582CD9A1B8DC652" modified="2001-10-01" version="1">MQIQRMRGNPHPDVITAAIAINPKIIKKSHKEHVDVETNEGLTRGVTIIDYVDPGHIIGNNKPNAEVVYDIDYEKFVQILLKTLSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UJ9</accession>
    <name>Q97UJ9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter, ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO3012</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="AAK43114.1"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="STRING" id="273057.SSO3012"/>
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        <property type="gene ID" value="SSO3012"/>
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    <dbReference type="GeneID" id="1453033"/>
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    <dbReference type="PATRIC" id="fig|273057.12.peg.3104"/>
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    <dbReference type="HOGENOM" id="CLU_000604_84_3_2"/>
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    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="P:transmembrane transport"/>
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    </dbReference>
    <dbReference type="CDD" id="cd18563">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ABC transporter, ATP binding protein"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
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    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
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        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015005">
        <property type="entry name" value="DUF1854"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 6"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ABC_tran"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF08909">
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    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
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    </dbReference>
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    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="135"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="452"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK43114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="863" mass="98506" checksum="2F63A0B3972FF0FD" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UL2</accession>
    <name>Q97UL2_SACS2</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">SSO11637</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
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            <taxon>Sulfolobaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11637"/>
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    <dbReference type="eggNOG" id="arCOG08503">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="68" mass="7975" checksum="DAA8BCE93A987947" modified="2001-10-01" version="1">MVYRQCIRWKKGLVNTQCEIEVQISDDDEVYVIKNGIVKRVKGENDIIPYINTISPAFRALVLYFVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UM8</accession>
    <name>Q97UM8_SACS2</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">SSO2976</name>
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        <lineage>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="GeneID" id="1452999"/>
    <dbReference type="KEGG" id="sso:SSO2976"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3067"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155676_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UM8"/>
    <dbReference type="PhylomeDB" id="Q97UM8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34314">
        <property type="entry name" value="CRENARCHAEAL PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34314:SF6">
        <property type="entry name" value="DUF3782 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="135" mass="15828" checksum="39CB85A3070A1960" modified="2001-10-01" version="1">MSLEDQVLKFLTDISRKEEEIEYLIVNMFKPQLESKGVNLASIKREFLVDDKGILRIGYVLPIMYYDQGEEIYLFLAKNYADYSTLEQLLIREKILENKFSKKVRSFLIAFAIPKKYYDMALKLGIEVISEKILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-11-27" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UR5</accession>
    <name>Q97UR5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2937</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90473">
        <property type="entry name" value="B90473"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UR5"/>
    <dbReference type="STRING" id="273057.SSO2937"/>
    <dbReference type="PaxDb" id="273057-SSO2937"/>
    <dbReference type="EnsemblBacteria" id="AAK43041">
        <property type="protein sequence ID" value="AAK43041"/>
        <property type="gene ID" value="SSO2937"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2937"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3025"/>
    <dbReference type="eggNOG" id="arCOG02855">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180514_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UR5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="5">
        <location>
            <begin position="12"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="111" mass="12823" checksum="14E4F25C60E859E5" modified="2001-10-01" version="1">MKTYIFFYIMLKVIEINGYPIMIYEKDGKEYTYLAGCPHKQRPITAEGYKINLDNIVCPFYNAVFSLITGELIEPPKSKTPRTPNCKLIRARLINGEVVFEDKPFVPRTKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UV4</accession>
    <name>Q97UV4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2888</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90467">
        <property type="entry name" value="E90467"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UV4"/>
    <dbReference type="STRING" id="273057.SSO2888"/>
    <dbReference type="PaxDb" id="273057-SSO2888"/>
    <dbReference type="EnsemblBacteria" id="AAK42996">
        <property type="protein sequence ID" value="AAK42996"/>
        <property type="gene ID" value="SSO2888"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2888"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2976"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2271163_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UV4"/>
    <dbReference type="PhylomeDB" id="Q97UV4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025471">
        <property type="entry name" value="DUF4322"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14210">
        <property type="entry name" value="DUF4322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4322" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="102" mass="11653" checksum="EA48A59DF50E3A09" modified="2001-10-01" version="1">MWNDSVESKSKGYNVSPQTVRNYVEEQGTEVIEKLLESMRRISMEILKGVKEVDISIDWTTKTWYGKPVEGLGSSAKGNSWNYYRDHEVSEYGAPPSFRSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UW5</accession>
    <name>Q97UW5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alcohol dehydrogenase (Zn containing) (Adh-13)</fullName>
            <ecNumber evidence="7">1.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">adh-13</name>
        <name evidence="7" type="ordered locus">SSO2878</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90465">
        <property type="entry name" value="H90465"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989821.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UW5"/>
    <dbReference type="STRING" id="273057.SSO2878"/>
    <dbReference type="PaxDb" id="273057-SSO2878"/>
    <dbReference type="EnsemblBacteria" id="AAK42983">
        <property type="protein sequence ID" value="AAK42983"/>
        <property type="gene ID" value="SSO2878"/>
    </dbReference>
    <dbReference type="GeneID" id="1452902"/>
    <dbReference type="KEGG" id="sso:SSO2878"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2962"/>
    <dbReference type="eggNOG" id="arCOG01455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_0_2"/>
    <dbReference type="InParanoid" id="Q97UW5"/>
    <dbReference type="PhylomeDB" id="Q97UW5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08259">
        <property type="entry name" value="Zn_ADH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Rhodanese" evidence="6">
        <location>
            <begin position="137"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="310" mass="33876" checksum="86D04C52C7253EDF" modified="2001-10-01" version="1">MKAAILTQFNSPFIIGNAEPHGVGVRINVAYTGICGRDLVIWKGGFKNLKLPLILGHEIVGYYNGRPVAVYPNLYCGSCDYCKSGKENLCDNARILGEGEITGGYAEQVIVPERNLIPLPDDKLEKYAATMDPVATAIHASKLADLKKDSKVLVTGAGGGVGIHLVQYLKYLGISNVYGLSSKGEKLKELGVTPVSDIRGEKFDAIFELVGSKTINDSLRALKKEGILVLIGNTEGEPITLSRPAMSIMRQHKIVGSASYTQAEYEEAIKLVGYGKIKAIYEIYELERINEAYRKMLERKVLGRAILKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UX6</accession>
    <name>Q97UX6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Muconolactone isomerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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    <dbReference type="HOGENOM" id="CLU_169014_0_0_2"/>
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    </dbReference>
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        <property type="entry name" value="MLI_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02426">
        <property type="entry name" value="MIase"/>
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    </dbReference>
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        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="104" mass="12021" checksum="C77131BF43AE9A15" modified="2001-10-01" version="1">MMLFLLWFKIKQPTNITQKQLMEIWKREAEAAMPAVKAGKIKGLYKVTGRREVVAIIDVNSHEELDGILETLPIVKELGHSLTIEVTAIHPYENFYELMKKLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UY1</accession>
    <name>Q97UY1_SACS2</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">SSO2858</name>
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        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
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            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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    </reference>
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        <property type="gene ID" value="SSO2858"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="185" mass="21164" checksum="64311CBCB15A18F9" modified="2001-10-01" version="1">MLPLTLIAILSLGIVEGLDPYKGLLFSYYFYSFRKVKESYVVPIVSTITYYIVGILIVEWLNISDNILTRGIMFSLLLVHVLIKLVIGKVLHYPSNMRPKILNVIQWSAINSIIQMNFIILLTLSILSKPSLILLPITVIIVRETTYCLSVKNNKILLKLTEYNFDYFYLITVLLLGIVIILPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V30</accession>
    <name>Q97V30_SACS2</name>
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        <submittedName>
            <fullName evidence="3">Quinol oxidase (SoxABC), cytochrome B subunit, putative (SoxC-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">soxC-like</name>
        <name evidence="3" type="ordered locus">SSO2805</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AAK42917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="F90457"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V30"/>
    <dbReference type="FunCoup" id="Q97V30">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2805"/>
    <dbReference type="PaxDb" id="273057-SSO2805"/>
    <dbReference type="EnsemblBacteria" id="AAK42917">
        <property type="protein sequence ID" value="AAK42917"/>
        <property type="gene ID" value="SSO2805"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2805"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2890"/>
    <dbReference type="eggNOG" id="arCOG01721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038033_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V30"/>
    <dbReference type="PhylomeDB" id="Q97V30"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.810.10:FF:000012">
        <property type="entry name" value="Cytochrome b/b6 domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.810.10">
        <property type="entry name" value="Cytochrome Bc1 Complex, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyt_b/b6_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005797">
        <property type="entry name" value="Cyt_b/b6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027387">
        <property type="entry name" value="Cytb/b6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053595">
        <property type="entry name" value="Cytochrome_b-type_ET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041076">
        <property type="entry name" value="cyt_b_SoxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19271">
        <property type="entry name" value="CYTOCHROME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19271:SF16">
        <property type="entry name" value="CYTOCHROME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13631">
        <property type="entry name" value="Cytochrom_B_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81648">
        <property type="entry name" value="a domain/subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="Transmembrane di-heme cytochromes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51002">
        <property type="entry name" value="CYTB_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
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            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        <location>
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    </feature>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="485"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="517"/>
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        </location>
    </feature>
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        <location>
            <begin position="11"/>
            <end position="213"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51002"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAK42917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="542" mass="60619" checksum="D5D26CE5C426C7D0" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V54</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2781</name>
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    <organism evidence="2 3">
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        <lineage>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90454">
        <property type="entry name" value="D90454"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V54"/>
    <dbReference type="STRING" id="273057.SSO2781"/>
    <dbReference type="PaxDb" id="273057-SSO2781"/>
    <dbReference type="EnsemblBacteria" id="AAK42891">
        <property type="protein sequence ID" value="AAK42891"/>
        <property type="gene ID" value="SSO2781"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2781"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2863"/>
    <dbReference type="eggNOG" id="arCOG01202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131320_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V54"/>
    <dbReference type="PhylomeDB" id="Q97V54"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052548">
        <property type="entry name" value="Type_VII_TA_antitoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933:SF1">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE MNTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="24"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="174" mass="20491" checksum="E2F1C48461BEB0CF" modified="2001-10-01" version="1">MFIRFLGFIYHMIKEPYAKLLNDMVKIMREEFKDDLISVVLYGSVARGDNRNDSDVDLLIVIDNLPMDGILKRIRLFETKVEDKLNLDEYWSKGYYISLSPILKTPEEAGKISPLYLDMVYDAVILYDKDQFFTKILQKLKGRLMELGAERVRMGKRWYWILKKDSGFGETVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V55</accession>
    <name>Q97V55_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90454">
        <property type="entry name" value="C90454"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V55"/>
    <dbReference type="PaxDb" id="273057-SSO2780"/>
    <dbReference type="EnsemblBacteria" id="AAK42890">
        <property type="protein sequence ID" value="AAK42890"/>
        <property type="gene ID" value="SSO2780"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2780"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2862"/>
    <dbReference type="eggNOG" id="arCOG01192">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153285_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V55"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="15"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="146" mass="17294" checksum="3A5D499CA6432A8B" modified="2001-10-01" version="1">MVKRLNFDSLAFSYITQAEERLDLAKTEYERKKYNIVVRLCQEAVELSLKACLRLVNIEPPKFHDVGPILKNNTDKFPQWFKEKIDIFASYSRSLRKERELSMYGDEETNTPPELLYSAYDGQQSIKIAEEILEYAKKLYEEKRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VD8</accession>
    <name>Q97VD8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfolobus mercury resistance protein, MerI</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90443">
        <property type="entry name" value="G90443"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988614.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VD8"/>
    <dbReference type="STRING" id="273057.SSO2690"/>
    <dbReference type="PaxDb" id="273057-SSO2690"/>
    <dbReference type="EnsemblBacteria" id="AAK42806">
        <property type="protein sequence ID" value="AAK42806"/>
        <property type="gene ID" value="SSO2690"/>
    </dbReference>
    <dbReference type="GeneID" id="1452725"/>
    <dbReference type="KEGG" id="sso:SSO2690"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2772"/>
    <dbReference type="eggNOG" id="arCOG07262">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2056152_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VD8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13817" checksum="5355AE974D354B60" modified="2001-10-01" version="1">MQEVKRLYEDEEVEEIALRITDEEIEVVDRRGVIKGKISSKQLAKKALEKKTLKFRITEAIDTKITKEDEKALPNLIELYFDPHLLIYGKKAEEVKGSGYICPICGIEIDEYGYCGCGTGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VE6</accession>
    <name>Q97VE6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type II secretion system protein, virB homolog (GspE-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gspE-2</name>
        <name evidence="3" type="ordered locus">SSO2680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90442">
        <property type="entry name" value="G90442"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923955.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VE6"/>
    <dbReference type="STRING" id="273057.SSO2680"/>
    <dbReference type="PaxDb" id="273057-SSO2680"/>
    <dbReference type="EnsemblBacteria" id="AAK42798">
        <property type="protein sequence ID" value="AAK42798"/>
        <property type="gene ID" value="SSO2680"/>
    </dbReference>
    <dbReference type="GeneID" id="1452717"/>
    <dbReference type="KEGG" id="sso:SSO2680"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2762"/>
    <dbReference type="eggNOG" id="arCOG01817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_2_2_2"/>
    <dbReference type="InParanoid" id="Q97VE6"/>
    <dbReference type="PhylomeDB" id="Q97VE6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004676">
        <property type="entry name" value="Type II secretion system protein E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF6">
        <property type="entry name" value="TYPE IV PILUS RETRACTATION ATPASE PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial type II secretion system protein E" evidence="2">
        <location>
            <begin position="191"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="515" mass="59089" checksum="E87A138B7A1FABBE" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VF3</accession>
    <name>Q97VF3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">N-acetylglucosamine-6-phosphate deacetylase (NagA)</fullName>
            <ecNumber evidence="6">3.5.1.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nagA</name>
        <name evidence="6" type="ordered locus">SSO2673</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. NagA family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.25" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90441">
        <property type="entry name" value="H90441"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VF3"/>
    <dbReference type="FunCoup" id="Q97VF3">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2673"/>
    <dbReference type="PaxDb" id="273057-SSO2673"/>
    <dbReference type="EnsemblBacteria" id="AAK42791">
        <property type="protein sequence ID" value="AAK42791"/>
        <property type="gene ID" value="SSO2673"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2673"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2755"/>
    <dbReference type="eggNOG" id="arCOG00699">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032482_2_1_2"/>
    <dbReference type="InParanoid" id="Q97VF3"/>
    <dbReference type="PhylomeDB" id="Q97VF3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008448">
        <property type="term" value="F:N-acetylglucosamine-6-phosphate deacetylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006046">
        <property type="term" value="P:N-acetylglucosamine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00854">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003764">
        <property type="entry name" value="GlcNAc_6-P_deAcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00221">
        <property type="entry name" value="nagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF14">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038994">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="5">
        <location>
            <begin position="77"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="395" mass="44033" checksum="791F211D00CEC3FF" modified="2001-10-01" version="1">MQWIYAKMKSPNYLNTRMKLRVSYTVRWVKVSKCIRGLEIITPLESFRDDIVISDGEIRKIGSGICNEEIKVDRGKYVIPGMIDIHTHGIGGILVNDIRSINDYEKMVSYYYSHGVTTFIPSTISENVEKLVSIARVLNEVKSIGIHLEGPLINPNRAGAHKFFTRFDERILEISKLFKIKRITIAPEILSDKELENLADNFQVSLGHTDANSDDTRRAIGFGASSVTHLFNAMRPFHHRDPGIIGVSLTSPIYAEVISDLVHINEITLSIVSKLKGDKTILVTDSLQAAGLGEGEFLLYGEKITCDKACFEANKRLVGSNITLDEGVRRVSKIIGLREAIKYVTYSPASLLNLEKIGQISRGYVADLVILDENLNVLTTLKSGSIVFSIFKNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VH2</accession>
    <name>Q97VH2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2653</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90439">
        <property type="entry name" value="E90439"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988564.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VH2"/>
    <dbReference type="FunCoup" id="Q97VH2">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2653"/>
    <dbReference type="PaxDb" id="273057-SSO2653"/>
    <dbReference type="EnsemblBacteria" id="AAK42772">
        <property type="protein sequence ID" value="AAK42772"/>
        <property type="gene ID" value="SSO2653"/>
    </dbReference>
    <dbReference type="GeneID" id="1452691"/>
    <dbReference type="KEGG" id="sso:SSO2653"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2735"/>
    <dbReference type="eggNOG" id="arCOG03056">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072573_10_1_2"/>
    <dbReference type="InParanoid" id="Q97VH2"/>
    <dbReference type="PhylomeDB" id="Q97VH2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01610">
        <property type="entry name" value="PAP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF13">
        <property type="entry name" value="AT30094P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="2">
        <location>
            <begin position="80"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="219" mass="24802" checksum="C6F5D1622C14A28A" modified="2001-10-01" version="1">MKWKYIAIIYALYIIISIVVKIIGEVNFLGNVYFFYLINHSQLPALNPIMIFFSKYGREYIWIPVTAILFIMGGKFRRSSLLLVGGFIIAIILGEVSKYVMAQPRPFLILHNINLLVPEPTDYSYPSGHALIVAVGAVIVLLTLPYYISIPLFIEALLTSYSRVYVGVHWPLDVLVGWILGIAIALTATNLEKLITTIYNRVVNLILGNMRKTNLKRPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VJ2</accession>
    <name>Q97VJ2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2631</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99437">
        <property type="entry name" value="A99437"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988533.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VJ2"/>
    <dbReference type="SMR" id="Q97VJ2"/>
    <dbReference type="STRING" id="273057.SSO2631"/>
    <dbReference type="PaxDb" id="273057-SSO2631"/>
    <dbReference type="EnsemblBacteria" id="AAK42752">
        <property type="protein sequence ID" value="AAK42752"/>
        <property type="gene ID" value="SSO2631"/>
    </dbReference>
    <dbReference type="GeneID" id="1452671"/>
    <dbReference type="KEGG" id="sso:SSO2631"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2714"/>
    <dbReference type="eggNOG" id="arCOG02065">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094970_2_0_2"/>
    <dbReference type="InParanoid" id="Q97VJ2"/>
    <dbReference type="PhylomeDB" id="Q97VJ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655">
        <property type="entry name" value="CONSERVED WITHIN P. AEROPHILUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DsrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="126" mass="14042" checksum="9B63806B3C67507B" modified="2001-10-01" version="1">MKTGIIVGSNERSRLTYAAMTSVIISSMGDEVYVFLTMDAVKGFTKNPEVKSEDISSKIMVEKKEEDYMSLFRKAKKSGKVKIYACSYASKLFGYAKDDYNDIVDEIAGITTFSMDVEGGQIISVW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VM8</accession>
    <name>TPIS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Triosephosphate isomerase</fullName>
            <shortName evidence="1">TIM</shortName>
            <shortName evidence="1">TPI</shortName>
            <ecNumber evidence="1">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tpiA</name>
        <name type="ordered locus">SSO2592</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90432">
        <property type="entry name" value="E90432"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989304.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VM8"/>
    <dbReference type="SMR" id="Q97VM8"/>
    <dbReference type="FunCoup" id="Q97VM8">
        <property type="interactions" value="271"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2592"/>
    <dbReference type="PaxDb" id="273057-SSO2592"/>
    <dbReference type="EnsemblBacteria" id="AAK42716">
        <property type="protein sequence ID" value="AAK42716"/>
        <property type="gene ID" value="SSO2592"/>
    </dbReference>
    <dbReference type="GeneID" id="1454043"/>
    <dbReference type="GeneID" id="44128322"/>
    <dbReference type="KEGG" id="sso:SSO2592"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2673"/>
    <dbReference type="eggNOG" id="arCOG01087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104921_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VM8"/>
    <dbReference type="PhylomeDB" id="Q97VM8"/>
    <dbReference type="BRENDA" id="5.3.1.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q97VM8"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000223">
        <property type="entry name" value="Triosephosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_A">
        <property type="entry name" value="TIM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022891">
        <property type="entry name" value="Triosephosphate_isomerase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003302">
        <property type="entry name" value="PRK04302.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139:SF42">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0312">Gluconeogenesis</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000090346" description="Triosephosphate isomerase">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="active site" description="Electrophile" evidence="1">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="201"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <sequence length="227" mass="24736" checksum="E29AE945AE6DE987" modified="2001-10-01" version="1">MKPPIIIINFKAYENSFGDKAVNLGKKIEKISKEYSVEIILSTPATMIYRMSQEVDLPIYAEHVDPVPLGAFTGAILPEMVKDAGAKGTLINHSERRLRADEIDDVLKRTKKLGLKSILCVDRYELVYPFSLLKPDAILIEPPELIGTGVSVSKAKPEVITRAVDEIRKSEGIYLIAGAGITTGEDVYKALKLGAHGIGVASAVMKAKEPEKVVEDFITSALRAISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VP3</accession>
    <name>Q97VP3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MarR family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2573</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90430">
        <property type="entry name" value="E90430"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991788.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VP3"/>
    <dbReference type="STRING" id="273057.SSO2573"/>
    <dbReference type="PaxDb" id="273057-SSO2573"/>
    <dbReference type="EnsemblBacteria" id="AAK42700">
        <property type="protein sequence ID" value="AAK42700"/>
        <property type="gene ID" value="SSO2573"/>
    </dbReference>
    <dbReference type="GeneID" id="1454027"/>
    <dbReference type="KEGG" id="sso:SSO2573"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2656"/>
    <dbReference type="eggNOG" id="arCOG03748">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1922885_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VP3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014572">
        <property type="entry name" value="UCP032673"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032673">
        <property type="entry name" value="UCP032673"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="15079" checksum="8A1354905D90D4E5" modified="2001-10-01" version="1">MNLREKILAYLYQNRDSTLEKISEGLKEDKYEVEATLRYLERDGLVIKRSKGIIFKKTIYDLTAAGLEEAKRIYETLKEKSIKLQELLQSGRLDPSKLPEEYVDIIPLLITMSLINMMLLEDLMLSDMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VP8</accession>
    <name>Q97VP8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rubrerythrin diiron-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2568</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90429">
        <property type="entry name" value="H90429"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991795.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VP8"/>
    <dbReference type="FunCoup" id="Q97VP8">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2568"/>
    <dbReference type="PaxDb" id="273057-SSO2568"/>
    <dbReference type="DNASU" id="1454022"/>
    <dbReference type="EnsemblBacteria" id="AAK42695">
        <property type="protein sequence ID" value="AAK42695"/>
        <property type="gene ID" value="SSO2568"/>
    </dbReference>
    <dbReference type="GeneID" id="1454022"/>
    <dbReference type="KEGG" id="sso:SSO2568"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2650"/>
    <dbReference type="eggNOG" id="arCOG01096">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065373_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VP8"/>
    <dbReference type="PhylomeDB" id="Q97VP8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02431">
        <property type="entry name" value="Ferritin_CCC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01044">
        <property type="entry name" value="Ferritin_CCC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039376">
        <property type="entry name" value="Ferritin_CCC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rr_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Rubrerythrin diiron-binding" evidence="6">
        <location>
            <begin position="7"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="355" mass="39066" checksum="FB6C7A209B7F9E90" modified="2001-10-01" version="1">MRKLENIILKNYEEELFDHEVYSKLAEVERDPELKKTLFKLAEMEKKHSEFWREIAEKRGLKVKGKLSTLNKIKVDFYSLLRKILGLDITIKVLESSEEDDIEKYRKLSEMEMFSSEEKQRLKDIMVDEAVHEKVLGNVEAKSVGDFVYGISDGLVEVLAAVSGLSGAISSPLFVAVGGLIVGVSGTLSMAIGAYLSTKSEKDVKIQERKRLELEKNVDQHSVQLRLVNFFTELGVNLNLAKKVSLSLVNVAEDILYPEVKENPVKSALITGLSYITGAIIPILPYLIGLSGLTGLITSYIVAGLSTFIVGSIIGILSGISPFKKGIQMASLALLAALGTHGLGYLASRFLNVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W46</accession>
    <name>Q97W46_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pantoate kinase</fullName>
            <shortName evidence="1">PoK</shortName>
            <ecNumber evidence="1">2.7.1.169</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2397</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylates (R)-pantoate to form (R)-4-phosphopantoate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantoate + ATP = (R)-4-phosphopantoate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28246"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.169"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. PoK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.169" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99411">
        <property type="entry name" value="A99411"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W46"/>
    <dbReference type="FunCoup" id="Q97W46">
        <property type="interactions" value="105"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2397"/>
    <dbReference type="PaxDb" id="273057-SSO2397"/>
    <dbReference type="EnsemblBacteria" id="AAK42544">
        <property type="protein sequence ID" value="AAK42544"/>
        <property type="gene ID" value="SSO2397"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2397"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2479"/>
    <dbReference type="eggNOG" id="arCOG04263">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081191_1_0_2"/>
    <dbReference type="InParanoid" id="Q97W46"/>
    <dbReference type="PhylomeDB" id="Q97W46"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02223">
        <property type="entry name" value="Pantoate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012043">
        <property type="entry name" value="PoK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42282:SF1">
        <property type="entry name" value="PANTOATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42282">
        <property type="entry name" value="PANTOATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016896">
        <property type="entry name" value="GHMP_arc_MJ0969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="2">
        <location>
            <begin position="84"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="271" mass="30018" checksum="506496701FEE6343" modified="2001-10-01" version="1">MMRLKSQLLLVKIINNSFIVLGVEIKVPISISGIWYPVIEKENLIKSGSIGLTLTLEPYITAEIRNGSGIEFNGIEIQLPNYEILKKKLGEYKLSVYSEVPLGYGYGLSGAISLAYALGVKELTPISEEDAVNTAHLSDVLSGNGLGDVIAQYYGGGLVYRKKAGGLGYGEVEVINMDWSYYPIFSQPISQLPTKSIIKRSEIALKLIDDFLKNPSPLKFIEVAQQFTSSLGFSSEYPYSYRKKGIIVKIFEPEYGVWIKHKIASRGAYVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WB9</accession>
    <name>Q97WB9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">One of two inversely orientated ORFs in ISC1043</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2314</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90401">
        <property type="entry name" value="F90401"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WB9"/>
    <dbReference type="PaxDb" id="273057-SSO2314"/>
    <dbReference type="EnsemblBacteria" id="AAK42469">
        <property type="protein sequence ID" value="AAK42469"/>
        <property type="gene ID" value="SSO2314"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2314"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2403"/>
    <dbReference type="eggNOG" id="arCOG09862">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2140319_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WB9"/>
    <dbReference type="PhylomeDB" id="Q97WB9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="117" mass="14011" checksum="5E675CFB4629E798" modified="2001-10-01" version="1">MYHEHMNKKLTQKDFDNFKESIEDLIKLYKESRLSKIENNKESEEERLARLRRFGRELRQDLEEAKFVKIKKEQGRKSKLTPQEKAYILIIKEYLGLSNRETAELASLLNLTKELPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WF4</accession>
    <name>Q97WF4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2272</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90397">
        <property type="entry name" value="B90397"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991513.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WF4"/>
    <dbReference type="STRING" id="273057.SSO2272"/>
    <dbReference type="PaxDb" id="273057-SSO2272"/>
    <dbReference type="EnsemblBacteria" id="AAK42433">
        <property type="protein sequence ID" value="AAK42433"/>
        <property type="gene ID" value="SSO2272"/>
    </dbReference>
    <dbReference type="GeneID" id="1453760"/>
    <dbReference type="KEGG" id="sso:SSO2272"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2363"/>
    <dbReference type="eggNOG" id="arCOG02050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_5_0_2"/>
    <dbReference type="InParanoid" id="Q97WF4"/>
    <dbReference type="PhylomeDB" id="Q97WF4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051598">
        <property type="entry name" value="TSUP/Inactive_protease-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN MJ0441-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701:SF2">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN YJNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="293" mass="31485" checksum="208DB8C9E03CD788" modified="2001-10-01" version="1">MFQDPTFQITVTQLQYLLSIISGILVGFSLGLIGGGGSILAIPLLLYFVGLANGIPPGSPEYTYITHLTLGTTALAVGLNSYINSYMHFRRGNVRVPEGIIFTIPGVIGDVIGAYLSHLMSGALILFLFGFLMIAVAIRMWRSKCNPNRSVLYNNSHKLSLRERIKVTKVIPAGFLVGFASGYFGIGGGFLVVPGLLFSTGLDMLRAVGTSLIAVGTFGVTAAITYAVYGYVDIVISLLYLLGGVVGGYTGSTIASRMPRQTLRKLFAIIIIVVAIYTMYINRVGVVELLHFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WM3</accession>
    <name>Q97WM3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2191</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90388">
        <property type="entry name" value="D90388"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992106.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WM3"/>
    <dbReference type="STRING" id="273057.SSO2191"/>
    <dbReference type="PaxDb" id="273057-SSO2191"/>
    <dbReference type="EnsemblBacteria" id="AAK42363">
        <property type="protein sequence ID" value="AAK42363"/>
        <property type="gene ID" value="SSO2191"/>
    </dbReference>
    <dbReference type="GeneID" id="1453690"/>
    <dbReference type="KEGG" id="sso:SSO2191"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2286"/>
    <dbReference type="eggNOG" id="arCOG03038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1010547_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WM3"/>
    <dbReference type="PhylomeDB" id="Q97WM3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13431">
        <property type="entry name" value="TPR_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="167"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="201"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="30888" checksum="97F599DF6AA925A6" modified="2001-10-01" version="1">MLILSKRLIDILSESLSLKEAYTQYKNKNLNYALKLLDELITQNPDLKEAYSLKGKILFELGKLQEAYENFQKAEDKIGMAKVLIAQGLYRDALIYLNDNSKDSRKLRGLIYLKLENYEKALDEIKVVEDEDPLVYKIRGISELKLGKYNDAIIDLSLAIEKYPTDAELYFYRALAQRELGNYKEAENDLEIAINLNPYYAEVYFSLGELKESRGLLEEAVDMYSKSININPLYRNAYVRRAKSLMKLGREEEAYADIRKANELNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WS6</accession>
    <name>Q97WS6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prolidase (Xaa-Pro dipeptidase) (PepQ-like2)</fullName>
            <ecNumber evidence="2">3.4.13.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pepQ-like2</name>
        <name evidence="2" type="ordered locus">SSO2040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.13.9" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90371">
        <property type="entry name" value="C90371"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923746.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WS6"/>
    <dbReference type="SMR" id="Q97WS6"/>
    <dbReference type="FunCoup" id="Q97WS6">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2040"/>
    <dbReference type="PaxDb" id="273057-SSO2040"/>
    <dbReference type="EnsemblBacteria" id="AAK42226">
        <property type="protein sequence ID" value="AAK42226"/>
        <property type="gene ID" value="SSO2040"/>
    </dbReference>
    <dbReference type="GeneID" id="7805882"/>
    <dbReference type="KEGG" id="sso:SSO2040"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2121"/>
    <dbReference type="eggNOG" id="arCOG00696">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023620_2_2_2"/>
    <dbReference type="InParanoid" id="Q97WS6"/>
    <dbReference type="PhylomeDB" id="Q97WS6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102009">
        <property type="term" value="F:proline dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01299">
        <property type="entry name" value="Met_dep_hydrolase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000120">
        <property type="entry name" value="Amidohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051781">
        <property type="entry name" value="Metallo-dep_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057744">
        <property type="entry name" value="OTAase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43135:SF3">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="49"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="408" mass="44712" checksum="B9477F6E8E4823DE" modified="2001-10-01" version="1">MELAIRNGIVFNGKEIIGKTNIYVREGKIVEVSREDHSAKMEIDAKDMFVMPGLIDAHIHLSGIKGGSLLKIMFEKPEYRVLRAAKWLEKLLLAGFTTVRDCGETVSLALKRALNDGIIGGPKVIAAGKPITQTFGHGELSHDVPLEFSKTMSFSEFCDGMESCIHAARKVLRDGADFIKIFATGGVLSQRDRPEHPQLSYEEIRAIVNEAEKVNTYVAAHAHGDRGARIAIEAGVKTLEHGTLLKDETLKLMKEKNVTLTPTLTIQELIFKYGKQIGVDEWGLQKITSVRENIANVVRKAKEYGVTIISGTDLGFETGLEEIDMGKNWMETVLLVERGGLSPIEALRASTYNAAMAIGINAGVIEIGKDADIIIINGDPTLNVREISKITHVVKNGKLTVENKSIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WT1</accession>
    <name>Q97WT1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90370">
        <property type="entry name" value="F90370"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923741.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WT1"/>
    <dbReference type="STRING" id="273057.SSO2035"/>
    <dbReference type="PaxDb" id="273057-SSO2035"/>
    <dbReference type="EnsemblBacteria" id="AAK42221">
        <property type="protein sequence ID" value="AAK42221"/>
        <property type="gene ID" value="SSO2035"/>
    </dbReference>
    <dbReference type="GeneID" id="1453548"/>
    <dbReference type="KEGG" id="sso:SSO2035"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2116"/>
    <dbReference type="eggNOG" id="arCOG03660">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726883_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WT1"/>
    <dbReference type="PhylomeDB" id="Q97WT1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052714">
        <property type="entry name" value="MFS_Exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531">
        <property type="entry name" value="QUINOLENE RESISTANCE PROTEIN NORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531:SF1">
        <property type="entry name" value="QUINOLENE RESISTANCE PROTEIN NORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="44008" checksum="F9502C24D2F5554D" modified="2001-10-01" version="1">MHSSKHSLIYSAIILVLMTISARATNNMVTTTVPLLAKYSLQLSNSLVGLLSALLFTFNFIATTFINPELNSRVRRRVFVISNVIILFSLPLYYLSNAISIWIISILAGIAFGLVMPNLITAASLANDKKSVERLLSLYSTSLSTSLILGPAIEVYLLTKYDYRDVFLWFIPIAVIGIIISSSIRFPDVKRESSGISVIKNKGLTAAALSITTYNVPFAAFTTFLAILAKDRFNLPNFDAYFVFLPFYIMSFLTRLTMTIRPFENLRMPMLISIIITILGLFGILYAPNYSIFLAVIALLGIPHGSIFPMATIMISRATSIAERNAVNSYFLAYNNLLFLVVPATIGFVSEIVGLSLSISALIIPVAITAVAFFKMFWNDNIMVKRYFVDLFNLSH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WX1</accession>
    <name>CMR6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR system CMR subunit Cmr6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>CRISPR type III-B/RAMP module RAMP protein Cmr6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cmr6</name>
        <name type="ordered locus">SSO1990</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="Mol. Cell" volume="45" first="303" last="313">
            <title>Structure and mechanism of the CMR complex for CRISPR-mediated antiviral immunity.</title>
            <authorList>
                <person name="Zhang J."/>
                <person name="Rouillon C."/>
                <person name="Kerou M."/>
                <person name="Reeks J."/>
                <person name="Brugger K."/>
                <person name="Graham S."/>
                <person name="Reimann J."/>
                <person name="Cannone G."/>
                <person name="Liu H."/>
                <person name="Albers S.V."/>
                <person name="Naismith J.H."/>
                <person name="Spagnolo L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="22227115"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2011.12.013"/>
        </citation>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION IN CMR COMPLEX</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). The CMR complex degrades RNA complementary to the crRNA (target RNA) within UA dinucleotides, generating 3'-OH and 5'-phosphate ends. Activity is dependent on the 8 nt long 5' tag in the crRNA, an unpaired 3' flag on the target RNA, and is stimulated by ATP. Some cleavage of the guide crRNA can also be observed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the CMR ribonucleoprotein complex, consisting of crRNA plus Cmr1/Cmr2/Cmr3/Cmr4/Cmr5/Cmr6 at 1:1 and possibly 3 Cmr7 dimers. A Cmr2/Cmr3/Cmr7 subcomplex without crRNA can also be isolated. It does not cleave target RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR system Cmr6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90365">
        <property type="entry name" value="E90365"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992993.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WX1"/>
    <dbReference type="SMR" id="Q97WX1"/>
    <dbReference type="STRING" id="273057.SSO1990"/>
    <dbReference type="PaxDb" id="273057-SSO1990"/>
    <dbReference type="EnsemblBacteria" id="AAK42180">
        <property type="protein sequence ID" value="AAK42180"/>
        <property type="gene ID" value="SSO1990"/>
    </dbReference>
    <dbReference type="GeneID" id="1453507"/>
    <dbReference type="GeneID" id="27428316"/>
    <dbReference type="KEGG" id="sso:SSO1990"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2066"/>
    <dbReference type="eggNOG" id="arCOG02661">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053305_3_0_2"/>
    <dbReference type="InParanoid" id="Q97WX1"/>
    <dbReference type="PhylomeDB" id="Q97WX1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010172">
        <property type="entry name" value="CRISPR-assoc_prot_TM1791"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01898">
        <property type="entry name" value="cas_TM1791_cmr6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39965">
        <property type="entry name" value="CRISPR SYSTEM CMR SUBUNIT CMR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39965:SF1">
        <property type="entry name" value="CRISPR SYSTEM CMR SUBUNIT CMR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418082" description="CRISPR system CMR subunit Cmr6">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="22227115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="284" mass="32458" checksum="58ACDC0DB2F01C8F" modified="2001-10-01" version="1">MKMSISTHLPRILDTLNRNNINLFSALSLTSIVYNNFGEFLSNKQSYSANNPLLKYQIIILKDKNKTKDVEEKKSLFKREIAELVSRNFKLEGEKVKKYFDNLKEILKSLKYTIVDFEITTRTRALIGVSTSLGKLIFDSGISFDPYMNLPYIPASEIKGIVRSYIEDKLGEQDAEEIFGNEEMEGNVNFTDAYPTRPENFLFVPDVITPHYNKKKSEADAEPKPAIHLTIAPKVSFRFLIYYKREDVGKPICDSLPLVIMKGLGARSSVGYSLFELSKIEVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WY0</accession>
    <name>Q97WY0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO9407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90364">
        <property type="entry name" value="D90364"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WY0"/>
    <dbReference type="STRING" id="273057.SSO9407"/>
    <dbReference type="PaxDb" id="273057-SSO9407"/>
    <dbReference type="EnsemblBacteria" id="AAK42171">
        <property type="protein sequence ID" value="AAK42171"/>
        <property type="gene ID" value="SSO9407"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO9407"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074024_2_0_2"/>
    <dbReference type="InParanoid" id="Q97WY0"/>
    <dbReference type="PhylomeDB" id="Q97WY0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="96" mass="11518" checksum="3FCA113935206413" modified="2001-10-01" version="1">MLKLYNKVRGPIETSYRNIKAFLPFTSSTKFVFRTLIFVLAVAFYSLYTVFKGEVRREQFRILLILLFSDDLFYLKDFLFKSIEPLINNIDLFSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X07</accession>
    <name>Q97X07_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposase ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1951</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90360">
        <property type="entry name" value="H90360"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923668.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X07"/>
    <dbReference type="PaxDb" id="273057-SSO1951"/>
    <dbReference type="EnsemblBacteria" id="AAK42143">
        <property type="protein sequence ID" value="AAK42143"/>
        <property type="gene ID" value="SSO1951"/>
    </dbReference>
    <dbReference type="GeneID" id="1453470"/>
    <dbReference type="KEGG" id="sso:SSO1951"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2025"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066381_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X07"/>
    <dbReference type="PhylomeDB" id="Q97X07"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="3">
        <location>
            <begin position="194"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="316" mass="35886" checksum="2674EF907453E578" modified="2001-10-01" version="1">MEAPVAGIDVSKDKLIVYFQGKLYEFTNDKRGYEEIRKILPKGCKVGIESTGVYHVNLAKYLMGEYDVRIINPFILKKFKDFRGKKSDKNDAKKLAELVVSMCSGFTTSDARELTSQWDFVTRSIARVKNRLRRDLVLLGYRDSLSKRNLEEVLRGGDSIVLAEVRFLLEELERLKTRKREIEKELENVVSKDSLIFTIPGIGKTLGCIILARVGDVKRFSDKKRFVAYCGLDPVVESSGKSVVSRGISKKGDAVLRRAFYLAALTAIKVNPVIKRFYEEHKGRLKGKKLIVACARKLAVITWAVLYYNKPFDASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X15</accession>
    <name>Q97X15_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90359">
        <property type="entry name" value="G90359"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992596.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X15"/>
    <dbReference type="STRING" id="273057.SSO1939"/>
    <dbReference type="PaxDb" id="273057-SSO1939"/>
    <dbReference type="EnsemblBacteria" id="AAK42134">
        <property type="protein sequence ID" value="AAK42134"/>
        <property type="gene ID" value="SSO1939"/>
    </dbReference>
    <dbReference type="GeneID" id="7807196"/>
    <dbReference type="KEGG" id="sso:SSO1939"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2014"/>
    <dbReference type="eggNOG" id="arCOG03169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061108_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X15"/>
    <dbReference type="PhylomeDB" id="Q97X15"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301:SF8">
        <property type="entry name" value="ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301">
        <property type="entry name" value="DNA-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="387" mass="44569" checksum="2FE04FA7C92D7D24" modified="2001-10-01" version="1">MLFNTRPKEDRKDLYDREKEIEMIKDSIARGEWIAVLGMRRIGKTSVVNVAVKEIGAIKVSINLMRIHDSRKKQYPKHVLISLLIEEINEAIKNYTILGKVAKLLSNILGVEEIQTNKVRAKLTKIRGTDITYIIREMDSIARDNKKQLVIILDEAQELAKVNGLDFPSIFHDVYDNCKNTVIIFTGSMVKLIEKTLKNIEYTEPFFGRYIRKITLGRFLPEQSREFLEKGFEEEGIKVDESVIDEAVKRLDGIPGWLTLFGSEYTFSAKMGTKPRIDEIIEKAINEVRNEARNFIFSTQSPLRYSAVILALDRLGGKGELHEIVKVSSTILNENIPEPRVYEILNRLVEFDFIERREDNEYYLHQDEPNRKGLILAAKDSPASYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X23</accession>
    <name>Q97X23_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Clan AA aspartic protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1929</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90358">
        <property type="entry name" value="F90358"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X23"/>
    <dbReference type="STRING" id="273057.SSO1929"/>
    <dbReference type="PaxDb" id="273057-SSO1929"/>
    <dbReference type="EnsemblBacteria" id="AAK42125">
        <property type="protein sequence ID" value="AAK42125"/>
        <property type="gene ID" value="SSO1929"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1929"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2004"/>
    <dbReference type="eggNOG" id="arCOG04029">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161799_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X23"/>
    <dbReference type="PhylomeDB" id="Q97X23"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022274">
        <property type="entry name" value="Peptidase_asp_AF0612"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03698">
        <property type="entry name" value="clan_AA_DTGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="15020" checksum="90C95EEEDF508AE4" modified="2001-10-01" version="1">MERVNSLECFDINEKPILHVKVSDPKNENSAEIDVLVNTGFSGWLLLNYEIYTKLNYMEIPITRKYRSILGNIEVYMAKASISINRLNIDAFIESSPYVEMNLLGREILKKLNICFYRMNKICIYSDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X25</accession>
    <name>Q97X25_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">First ORF in partial transposon ISC1913</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1927</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90358">
        <property type="entry name" value="D90358"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X25"/>
    <dbReference type="STRING" id="273057.SSO1927"/>
    <dbReference type="PaxDb" id="273057-SSO1927"/>
    <dbReference type="EnsemblBacteria" id="AAK42123">
        <property type="protein sequence ID" value="AAK42123"/>
        <property type="gene ID" value="SSO1927"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1927"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_2_0_2"/>
    <dbReference type="InParanoid" id="Q97X25"/>
    <dbReference type="PhylomeDB" id="Q97X25"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04762">
        <property type="entry name" value="HTH_MerR-trunc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1660.10:FF:000012">
        <property type="entry name" value="DNA-binding protein, excisionase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1390:FF:000002">
        <property type="entry name" value="ORF1 in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="4">
        <location>
            <begin position="58"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="145" mass="17078" checksum="71C51A417A9EEEE3" modified="2001-10-01" version="1">MERLLRPKEACQLLGISYSTLLRWIREGKIRVVTTEGGKYRIPYSEIKKYLERREEPRAVIYARVSSADQKEDLERQINYLTNYATAKGYKVVEVLKDIASGLNTQRKGLLKLFKLVEGSFRYKNFSLKIEHNSHYLRNETCFTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X30</accession>
    <name>Q97X30_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dTDP-4-dehydrorhamnose 3,5-epimerase</fullName>
            <ecNumber evidence="2">5.1.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Thymidine diphospho-4-keto-rhamnose 3,5-epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rfbC-2</name>
        <name evidence="3" type="ordered locus">SSO1923</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose</text>
            <dbReference type="Rhea" id="RHEA:16969"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="ChEBI" id="CHEBI:62830"/>
            <dbReference type="EC" id="5.1.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.13" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90357">
        <property type="entry name" value="G90357"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988450.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X30"/>
    <dbReference type="FunCoup" id="Q97X30">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1923"/>
    <dbReference type="PaxDb" id="273057-SSO1923"/>
    <dbReference type="EnsemblBacteria" id="AAK42118">
        <property type="protein sequence ID" value="AAK42118"/>
        <property type="gene ID" value="SSO1923"/>
    </dbReference>
    <dbReference type="GeneID" id="1453445"/>
    <dbReference type="KEGG" id="sso:SSO1923"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1998"/>
    <dbReference type="eggNOG" id="arCOG04188">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090940_1_1_2"/>
    <dbReference type="InParanoid" id="Q97X30"/>
    <dbReference type="PhylomeDB" id="Q97X30"/>
    <dbReference type="UniPathway" id="UPA00124"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008830">
        <property type="term" value="F:dTDP-4-dehydrorhamnose 3,5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016854">
        <property type="term" value="F:racemase and epimerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00438">
        <property type="entry name" value="cupin_RmlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000888">
        <property type="entry name" value="RmlC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01221">
        <property type="entry name" value="rmlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21047">
        <property type="entry name" value="DTDP-6-DEOXY-D-GLUCOSE-3,5 EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21047:SF2">
        <property type="entry name" value="THYMIDINE DIPHOSPHO-4-KETO-RHAMNOSE 3,5-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00908">
        <property type="entry name" value="dTDP_sugar_isom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="site" description="Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucose" evidence="1">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU364069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="176" mass="20307" checksum="8FB8F54976DA22EE" modified="2001-10-01" version="1">MPFEFENLGMGIVLIKPKVFPDKRGFFLEVFKSEDFTKAEIPNIVQTNMSFSFKGVVRGLHYQITPKEQGKLVFVPKGKILDVALDLRKSSPTFGKYVKVELNDENHYILWIPPGFAHGFQALEDSIVVYFLTHNEYSKSDERCVNYSYVDWPIKEFIISDKDSQCPPLEKADVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X38</accession>
    <name>Q97X38_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PaREP1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1917</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90356">
        <property type="entry name" value="G90356"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X38"/>
    <dbReference type="SMR" id="Q97X38"/>
    <dbReference type="STRING" id="273057.SSO1917"/>
    <dbReference type="PaxDb" id="273057-SSO1917"/>
    <dbReference type="DNASU" id="1453437"/>
    <dbReference type="EnsemblBacteria" id="AAK42110">
        <property type="protein sequence ID" value="AAK42110"/>
        <property type="gene ID" value="SSO1917"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1917"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1982"/>
    <dbReference type="eggNOG" id="arCOG03712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2217171_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X38"/>
    <dbReference type="PhylomeDB" id="Q97X38"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="12490" checksum="10459BE2140C273A" modified="2001-10-01" version="1">MERKWIKKKAHIVPTHAMYGLAQVLKDIGIDVISLVNYALNLHDYHYNGFEPGFSRYSKKEEVFRDLITLVKETRKVIDIYYSKYEVKEILGKINELIKELTEGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X69</accession>
    <name>Q97X69_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial ORF from ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO9135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90352">
        <property type="entry name" value="B90352"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X69"/>
    <dbReference type="STRING" id="273057.SSO9135"/>
    <dbReference type="PaxDb" id="273057-SSO9135"/>
    <dbReference type="EnsemblBacteria" id="AAK42073">
        <property type="protein sequence ID" value="AAK42073"/>
        <property type="gene ID" value="SSO9135"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO9135"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1938"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_3_0_2"/>
    <dbReference type="InParanoid" id="Q97X69"/>
    <dbReference type="PhylomeDB" id="Q97X69"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="34"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="79" mass="9367" checksum="4345928D8722DA0A" modified="2001-10-01" version="1">MNKGYIKPVILQNGKWRFREEDVEKLMGIVRRRKIVLYARVPSSTQKDELVNQVKYLEEQVKEYDLVIIDVGSALNMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XB6</accession>
    <name>Q97XB6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Second ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1829</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90346">
        <property type="entry name" value="B90346"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923615.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XB6"/>
    <dbReference type="STRING" id="273057.SSO1829"/>
    <dbReference type="PaxDb" id="273057-SSO1829"/>
    <dbReference type="EnsemblBacteria" id="AAK42025">
        <property type="protein sequence ID" value="AAK42025"/>
        <property type="gene ID" value="SSO1829"/>
    </dbReference>
    <dbReference type="GeneID" id="1453352"/>
    <dbReference type="KEGG" id="sso:SSO1829"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1889"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="InParanoid" id="Q97XB6"/>
    <dbReference type="PhylomeDB" id="Q97XB6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF22">
        <property type="entry name" value="TNPB-LIKE PROTEIN MJ1635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="187"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="308"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="419" mass="50005" checksum="DEE848F7CB3BB1BC" modified="2001-10-01" version="1">MLKNLRIRKFEPEEEYVYFTYSIKNSEREKSKELIKEYRTLLQKAIDHLWNLTKIQVRKNGNYKITLPKKKEVYKPLREELEKINHLASHYVDKAINDAFSILTSWRKRAIKGRASIEKPRVKKAYVRIKTTLRKVVGESVRITVRPYEYITFSWSKSWFSRRVRELELGEPIIKEEKVYLPFRYKLPWVTPLNFLAIDSNLYTLDAYDGEKFVTISLKQLYSLKYSMEVKRAKVQSFASKHTKRGRELMRKYSHRERNRVLDFVHKFVNTLLDLYPMTFFAVEKLNKESMFKDANDSLSRKISRTVWRSIHRVLKYKAPLYGSFVKEVNPYLTSRSCPRCRFVSRKVGKTFECERCGFKLDRQLNASLNIYLKMCGFPHIRSRVWVGVIPLMGRRGMNVRDFGEAQGLRVDIKYHEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XD0</accession>
    <name>Q97XD0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1234</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1814</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90344">
        <property type="entry name" value="D90344"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923605.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XD0"/>
    <dbReference type="STRING" id="273057.SSO1814"/>
    <dbReference type="PaxDb" id="273057-SSO1814"/>
    <dbReference type="EnsemblBacteria" id="AAK42011">
        <property type="protein sequence ID" value="AAK42011"/>
        <property type="gene ID" value="SSO1814"/>
    </dbReference>
    <dbReference type="GeneID" id="1454790"/>
    <dbReference type="KEGG" id="sso:SSO1814"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1873"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075479_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XD0"/>
    <dbReference type="PhylomeDB" id="Q97XD0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="319" mass="36760" checksum="49657D3252D1B0CA" modified="2001-10-01" version="1">MGVEGSTMARTLRHIPNLMYYPLPPIEDMPWREKWLTEIKPVLDAMDLERVLGEGALVYLKLLIVMVLYSCSYRDAVKMVNVNVVVAWFVGRKVGKSTLHDFVGRLYGVRKKLLEISFKLEEKCLPSYLPASAHLVDFMAWLVDSFLLDLPPGKRSVETFREKAELERREGNLERARKLLSLGRTKRRFEGRWTKKRGVSHYGLKAVAVISVSLFVRSITVKPANFSDKRFKSPLKGIKIADRGFSPSPTQLIAREKPFTTLRAHVEFFGTYLNAFWRPYGTTTWRNDVFLHVLGVIYNIKMFLAIQRRTPPGRRAVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XD8</accession>
    <name>Q97XD8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF981 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90343">
        <property type="entry name" value="D90343"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923603.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XD8"/>
    <dbReference type="PaxDb" id="273057-SSO1807"/>
    <dbReference type="DNASU" id="1454782"/>
    <dbReference type="EnsemblBacteria" id="AAK42003">
        <property type="protein sequence ID" value="AAK42003"/>
        <property type="gene ID" value="SSO1807"/>
    </dbReference>
    <dbReference type="GeneID" id="1454782"/>
    <dbReference type="KEGG" id="sso:SSO1807"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1865"/>
    <dbReference type="eggNOG" id="arCOG04349">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1363701_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XD8"/>
    <dbReference type="PhylomeDB" id="Q97XD8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009324">
        <property type="entry name" value="DUF981"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06168">
        <property type="entry name" value="DUF981"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="23105" checksum="01AFF201DFBD2F8E" modified="2001-10-01" version="1">MVFIDNLGLMLFILPLVSVLIAYTTVQAFIGFRKTGYEAIKRSLDDSILPAAILGTIIIILSLWGEFSWTLPGSYNILFYDVYLLLGITILSYSVTVYMGKRLQSTGIFALFVGLITIYYGASGYNLGLTKEPLALLALYTTYGIAGILGYPVTLALDRLRDQQGVKDPKLEGWMYAFFILFWIFVIIAGIIGAFIAVETIPAHLAHPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XE7</accession>
    <name>Q97XE7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CopG family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO8938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90342">
        <property type="entry name" value="C90342"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992863.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XE7"/>
    <dbReference type="PaxDb" id="273057-SSO8938"/>
    <dbReference type="EnsemblBacteria" id="AAK41994">
        <property type="protein sequence ID" value="AAK41994"/>
        <property type="gene ID" value="SSO8938"/>
    </dbReference>
    <dbReference type="GeneID" id="1454773"/>
    <dbReference type="KEGG" id="sso:SSO8938"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1855"/>
    <dbReference type="eggNOG" id="arCOG08305">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2662403_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XE7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="43"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="9054" checksum="0020B6AEABAA8F59" modified="2001-10-01" version="1">MPRPGYKSVYFPDEELWKKIVDEAEKRKVSVYEVLKDAFECYMKEKEGNKISLEEVNKELQELKKRVEELEKKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XK7</accession>
    <name>Q97XK7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR type III-associated protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1726</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90333">
        <property type="entry name" value="F90333"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923683.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XK7"/>
    <dbReference type="SMR" id="Q97XK7"/>
    <dbReference type="STRING" id="273057.SSO1726"/>
    <dbReference type="PaxDb" id="273057-SSO1726"/>
    <dbReference type="EnsemblBacteria" id="AAK41925">
        <property type="protein sequence ID" value="AAK41925"/>
        <property type="gene ID" value="SSO1726"/>
    </dbReference>
    <dbReference type="GeneID" id="1454704"/>
    <dbReference type="KEGG" id="sso:SSO1726"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1769"/>
    <dbReference type="eggNOG" id="arCOG02657">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047795_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XK7"/>
    <dbReference type="PhylomeDB" id="Q97XK7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09682">
        <property type="entry name" value="Cmr4_III-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013410">
        <property type="entry name" value="CRISPR-assoc_RAMP_Cmr4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02580">
        <property type="entry name" value="cas_RAMP_Cmr4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36700">
        <property type="entry name" value="CRISPR SYSTEM CMR SUBUNIT CMR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36700:SF1">
        <property type="entry name" value="CRISPR SYSTEM CMR SUBUNIT CMR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="2">
        <location>
            <begin position="10"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="295" mass="32723" checksum="096C793F70538DF4" modified="2001-10-01" version="1">MNGLFVLAYAITNLHPGAGRGYGIVDMPVQRDQMGYPIIYSSAFKGPLKALCAGDAINDHGRIDCKKKGECCCLFGSEPEEEESSKGMLNVMDLPLFSVPAPTFDGFVQVTTNYLIDRARNIFEAISHINGYLGSLGNEIKALLDAQGNKSVEVYDTEAKVVNFDPSSFKLLSSLPLFNNRLAISDDKRVIDKSLIIYTRNRLNLLTKVVEEGGLWSEEYIPMGSIFIGGMIIDNVRNKFCDELSKISCDSRLFLHHFSRIFNADKISEDVYLFYLNIGGKESIGKGLMKVFIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XN5</accession>
    <name>Q97XN5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposase ISC1250</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1679</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90329">
        <property type="entry name" value="A90329"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923560.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XN5"/>
    <dbReference type="PaxDb" id="273057-SSO1679"/>
    <dbReference type="EnsemblBacteria" id="AAK41888">
        <property type="protein sequence ID" value="AAK41888"/>
        <property type="gene ID" value="SSO1679"/>
    </dbReference>
    <dbReference type="GeneID" id="1454667"/>
    <dbReference type="KEGG" id="sso:SSO1679"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1726"/>
    <dbReference type="eggNOG" id="arCOG04918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074510_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XN5"/>
    <dbReference type="PhylomeDB" id="Q97XN5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217:SF7">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00872">
        <property type="entry name" value="Transposase_mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="342" mass="39477" checksum="83BF60832828FFF7" modified="2001-10-01" version="1">METITIDEKTLYNLTQPSEVIEELTHLNGNLTLVPNILSEDALLNKSVKEIEKIALEEADKTSPKHVRGKKIRRKGYAKYRFLKGFKIMLKGREDIYKLERISVELPVLYSDKGRVKTQVEERLLREEKVFKALMTVYSVLGGKLNAKLWMLEIQASGDFKYVIVDGKYVKLKGGRGSFSAIGVTEEGKRAVLDITLSVEKDVMSYWRLLVEVWKKYSFVLVVVDGTKALDKISLAELQVRRQGCLVHLKRNATKEEREALNVIFSAESGEIKPETCPRLLSYLSTPKELWRWLKSNNLIESFNSLLERRRFGSFHSPWRILQIARTIANNYNLSVVFHQFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XV5</accession>
    <name>Q97XV5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Third ORF in transposon ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1603</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90319">
        <property type="entry name" value="G90319"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XV5"/>
    <dbReference type="STRING" id="273057.SSO1603"/>
    <dbReference type="PaxDb" id="273057-SSO1603"/>
    <dbReference type="EnsemblBacteria" id="AAK41814">
        <property type="protein sequence ID" value="AAK41814"/>
        <property type="gene ID" value="SSO1603"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1603"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1641"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1302673_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XV5"/>
    <dbReference type="PhylomeDB" id="Q97XV5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="1">
        <location>
            <begin position="51"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="172" mass="20055" checksum="C0B9B07B9B995186" modified="2001-10-01" version="1">MGDFTQEELYQLEYTPIVVEEIKVLAKTLLETQERLKEVRRMIEEQVPDNHVLLTIPGIGRLAAGIIIGIVGDIRRFPKPESFVAYCGLDPVVERSGRAVISRGISKRGNKYLRSLFYFLAVRNYSRNPTLLKFYETHKDRLKGRKLYVALARKLARIIWSVWYNNRPYEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XW2</accession>
    <name>Q97XW2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycerol kinase (GlpK-1)</fullName>
            <ecNumber evidence="4">2.7.1.30</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">glpK-1</name>
        <name evidence="4" type="ordered locus">SSO1599</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.1.30" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90318">
        <property type="entry name" value="H90318"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923496.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XW2"/>
    <dbReference type="STRING" id="273057.SSO1599"/>
    <dbReference type="PaxDb" id="273057-SSO1599"/>
    <dbReference type="EnsemblBacteria" id="AAK41807">
        <property type="protein sequence ID" value="AAK41807"/>
        <property type="gene ID" value="SSO1599"/>
    </dbReference>
    <dbReference type="GeneID" id="1454586"/>
    <dbReference type="KEGG" id="sso:SSO1599"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1635"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074024_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XW2"/>
    <dbReference type="PhylomeDB" id="Q97XW2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025471">
        <property type="entry name" value="DUF4322"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14210">
        <property type="entry name" value="DUF4322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4322" evidence="3">
        <location>
            <begin position="2"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="77"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="294" mass="34371" checksum="9457C4E2F7270288" modified="2001-10-01" version="1">MITLDLPHQNNTQQIGYKLLSILDFQGKKAENLETLKGEKEIDISIDWTTKTWYGKPVEVSSEKGNSWNYATEMTKHNGKVLLLAFVTQVNEMTKDYIVKILVEQVTAMGFKIRLITLDAGFYTVDVLNFISQFKYIIAVPVGDMKVYEEFDGEYMTNSKRHRRDEQVKFRLLVYGREKIKRKKVVYFARGTNLDLPKKEALKLYDKVRSPIETSYRNIKALLPFTSSTKFVFHMLIFVLAMIFYSLYTVFKGMARREEFRLLLILLFPGDLFNLENSLFKLLETLINTIDLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XZ2</accession>
    <name>Q97XZ2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Heme biosynthesis related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1566</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90315">
        <property type="entry name" value="D90315"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989989.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XZ2"/>
    <dbReference type="STRING" id="273057.SSO1566"/>
    <dbReference type="PaxDb" id="273057-SSO1566"/>
    <dbReference type="EnsemblBacteria" id="AAK41779">
        <property type="protein sequence ID" value="AAK41779"/>
        <property type="gene ID" value="SSO1566"/>
    </dbReference>
    <dbReference type="GeneID" id="1454558"/>
    <dbReference type="KEGG" id="sso:SSO1566"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1601"/>
    <dbReference type="eggNOG" id="arCOG00945">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_3_1_2"/>
    <dbReference type="InParanoid" id="Q97XZ2"/>
    <dbReference type="PhylomeDB" id="Q97XZ2"/>
    <dbReference type="UniPathway" id="UPA00782"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023867">
        <property type="entry name" value="Sulphatase_maturase_rSAM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787:SF3">
        <property type="entry name" value="ARYLSULFATASE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787">
        <property type="entry name" value="FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01384">
        <property type="entry name" value="thioether_bond_formation_requi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="66"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="394" mass="45513" checksum="BD10020C52679BEE" modified="2001-10-01" version="1">MALSKFNLFIGDILFNTLTGYAIKLESWEIEKLRKGVVPEHLKDIIEEGFSATDEDLDEEIEKFLRKPVLEPTLVLTYNCNFDCIYCFQKGFRKNISVSDKVTRGFVNYIRKNEKGRKVRVTYFGGEPILQLRKIEEISRELSDLKYSFSVVTNGSLLTRKIVERLIPLGLTHVQITLDGPKEVHDKRRFFVSGEGTFDVIVKNLKEIQDMVKVVLRINIDVKNLNEIGELLNELKMEGINRVRLDPHLVHSNVFRNEWWDFTISSKAEGDVMVKFWEIARKYGFEVPQDVLRLGLCVAYSSEDIVVDPEGNIYPCWAFTGNPLYVKGKLEEDGSVKVTNKSLWWKVKIEDKCRECPFLPLCMGGCKFLATINGDDCKREAYEKIVRSIRWVMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XZ9</accession>
    <name>Q97XZ9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">First ORF in transposon ISC1491</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1556</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90314">
        <property type="entry name" value="E90314"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XZ9"/>
    <dbReference type="STRING" id="273057.SSO1556"/>
    <dbReference type="PaxDb" id="273057-SSO1556"/>
    <dbReference type="EnsemblBacteria" id="AAK41772">
        <property type="protein sequence ID" value="AAK41772"/>
        <property type="gene ID" value="SSO1556"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1556"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1592"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098916_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XZ9"/>
    <dbReference type="PhylomeDB" id="Q97XZ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="2">
        <location>
            <begin position="12"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="189"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="245" mass="28343" checksum="D5D2AD2D4E785DA5" modified="2001-10-01" version="1">MESEIKTRGFAIDVSRDKLTVAKAELVITQEKREVEVNEVREFKYDNEGIEELIKFLGEYKEGIMESTGPYFFYLHEELTEKGYKVTLINPLHLKEVFGKKTDKLDAQRLAKAFILGAVKGSYIPTGEIRELRELTRYRESLMRKITQVKNEIRKFLEMAGYKIEPFDKRGIALLEKLSRGEGLSKEERDELKEKLGRSLNDAEKLALKQLVDLLKSLEAMVKEVEDMIISKIPQPVVELSRELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y05</accession>
    <name>Q97Y05_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90313">
        <property type="entry name" value="G90313"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923462.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y05"/>
    <dbReference type="PaxDb" id="273057-SSO1550"/>
    <dbReference type="EnsemblBacteria" id="AAK41766">
        <property type="protein sequence ID" value="AAK41766"/>
        <property type="gene ID" value="SSO1550"/>
    </dbReference>
    <dbReference type="GeneID" id="71027442"/>
    <dbReference type="KEGG" id="sso:SSO1550"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1586"/>
    <dbReference type="eggNOG" id="arCOG09934">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_705182_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y05"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="44903" checksum="45A0AD48212D137A" modified="2001-10-01" version="1">MKLLESKPSKILSNTNVKFYLLQHFLQPVIIISAVFPAYLFLQTKNPLLVGALLSISYVLGHLSGISGFLTDYIVNTRRYDLILSLLSGTSFLLFYFLYRFELGFIFLAYTVIVTIHSFKGTNHVKVLRNLVNSASEYRVVWITVNIVRTASAIVFLYLTFFIIGLRSLLLVLGLFMLLIHTPLIFKIKYPKGTGNAKLLFSLSISAFKKDRAITFFLIDTLFANFIGGLFPTFLVFFPQSYFVLNYMIYYSMTIAYSSISLLIFTKIKVEKYRNNLYYIFIFFRGLIPAILLVSLAFVQPQFSLILIVFYWLSLTLYSSFASPIFQMKLPKEIIASYNGLLATLGSIFGALGNILLSFLDLLVGQKDVVIVVSELLLGYIVLALWVFKNVFKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-10-02" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y31</accession>
    <name>Q97Y31_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Partial transposase from ISC1048</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1518</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90310">
        <property type="entry name" value="E90310"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y31"/>
    <dbReference type="EnsemblBacteria" id="AAK41740">
        <property type="protein sequence ID" value="AAK41740"/>
        <property type="gene ID" value="SSO1518"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1518"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1558"/>
    <dbReference type="eggNOG" id="arCOG02130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124929_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y31"/>
    <dbReference type="PhylomeDB" id="Q97Y31"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13565">
        <property type="entry name" value="HTH_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="108" mass="12718" checksum="FE8FB31DEB4E65BD" modified="2001-10-01" version="1">MQDLVQAYKEEKNARIKERILAVKLHVVDGKSEKEVSKMLNKGYSTIKLWIGKYKKEGLDGLKDKPRSGRPRKVEEEKIKQILEDKPQKYGIQQEYWTMKTLKIALQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y48</accession>
    <name>Q97Y48_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Second ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1498</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90308">
        <property type="entry name" value="D90308"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y48"/>
    <dbReference type="STRING" id="273057.SSO1498"/>
    <dbReference type="PaxDb" id="273057-SSO1498"/>
    <dbReference type="EnsemblBacteria" id="AAK41723">
        <property type="protein sequence ID" value="AAK41723"/>
        <property type="gene ID" value="SSO1498"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1498"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1541"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="InParanoid" id="Q97Y48"/>
    <dbReference type="PhylomeDB" id="Q97Y48"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF22">
        <property type="entry name" value="TNPB-LIKE PROTEIN MJ1635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="187"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="308"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="400" mass="47318" checksum="CA33643BAAFF3A60" modified="2001-10-01" version="1">MLKNLRIRKFEPEEKYAYFTYSIKNDKREKSKELIKEYRTLLQKAIDYLWNLTKIQVRKNGNYKITLPKKREVYKPLREELEKINHLASHYVDKAINDAFSILKSWRKRAIKGRASIGKPRVKKAYVRIKTTLRKVVGESVRITVRPYEYITFSWSKSWFSRRVRELELGEPVIKEEKVYLSFRYKLPWATPVNFLAIDSNLYTLDAYDGEKFVTISLKQLYSLKYSMEVKRAKVQSFASKHTKRGRELLRKYSHRERNRVLDFVHKFVNALLDLYPMTFFAVEKLDKEGMFVDANESLSRKISLTVWGSIHRVLRYKAPLYGSFVKEVNPRFTSRSCPRCGFVSRKVGKTFECERCGFKLDRQLNASLNIYLKMCGFPHIREGGVRGYSANGAERDERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y56</accession>
    <name>Q97Y56_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1489</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90307">
        <property type="entry name" value="D90307"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y56"/>
    <dbReference type="PaxDb" id="273057-SSO1489"/>
    <dbReference type="EnsemblBacteria" id="AAK41715">
        <property type="protein sequence ID" value="AAK41715"/>
        <property type="gene ID" value="SSO1489"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1489"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1527"/>
    <dbReference type="HOGENOM" id="CLU_1607234_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y56"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="170" mass="19265" checksum="4A156CE58F514C00" modified="2001-10-01" version="1">MNSITMSKKESYKELVEKSDVKVLKQVQLKDGYYVMEDRHVLTHSLLHKIKTSLDDTKVITTVTQRDDGSLRITLFMYKVVGDKDEQGFYTIKSRGIYINIKGDNVEIFEGGPAEVKSPLIESNPGEEKEVLLKAITKGTENEMFSRFLEVNGSSGLTDLVKKTNFHKVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YA7</accession>
    <name>Q97YA7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR type III-associated protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1425</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2023" name="Curr. Res. Struct. Biol." volume="5" first="100098" last="100098">
            <title>Structure of the &lt;i&gt;Saccharolobus solfataricus&lt;/i&gt; type III-D CRISPR effector.</title>
            <authorList>
                <person name="Cannone G."/>
                <person name="Kompaniiets D."/>
                <person name="Graham S."/>
                <person name="White M.F."/>
                <person name="Spagnolo L."/>
            </authorList>
            <dbReference type="PubMed" id="36843655"/>
            <dbReference type="DOI" id="10.1016/j.crstbi.2023.100098"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (3.50 ANGSTROMS) OF 3-250</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90300">
        <property type="entry name" value="D90300"/>
    </dbReference>
    <dbReference type="PDB" id="8BMW">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="F/G=3-250"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YA7"/>
    <dbReference type="SMR" id="Q97YA7"/>
    <dbReference type="STRING" id="273057.SSO1425"/>
    <dbReference type="PaxDb" id="273057-SSO1425"/>
    <dbReference type="EnsemblBacteria" id="AAK41659">
        <property type="protein sequence ID" value="AAK41659"/>
        <property type="gene ID" value="SSO1425"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1425"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1451"/>
    <dbReference type="eggNOG" id="arCOG02658">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084207_1_0_2"/>
    <dbReference type="InParanoid" id="Q97YA7"/>
    <dbReference type="PhylomeDB" id="Q97YA7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013411">
        <property type="entry name" value="CRISPR-assoc_RAMP_Csx7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052216">
        <property type="entry name" value="CRISPR_Csm3_endoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02581">
        <property type="entry name" value="cas_cyan_RAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35579">
        <property type="entry name" value="CRISPR SYSTEM CMS ENDORIBONUCLEASE CSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35579:SF6">
        <property type="entry name" value="DUF324 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="5" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="2">
        <location>
            <begin position="43"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="5"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="82"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="107"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="8BMW"/>
        </source>
    </evidence>
    <sequence length="250" mass="27939" checksum="F005D86E641A38DD" modified="2001-10-01" version="1">MKMSCMDLDVITTVVKIEGKLRNETLLRVGKGKTQDFAEATDNPIIKYRDRPLIPGSSLKGAFRSLVESYTKSLNDSKYYVCDLDDNSCVSCEEKKKDNGIVEGRYCIPCILFGFKDLASRVYILDAIAEKYSISQRTMVAINRVFGGQMPGHLYTLDYVDPGSEFSFMMMIYNLNLIEGEKDWKAKSVEALKFLLATLVREGIFVGARKSVGYGLIKLVDAKVSLYKAPDHLVSPVIVKKLEEVIGTNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YD3</accession>
    <name>Q97YD3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">CRISPR-associated exonuclease Cas4</fullName>
            <ecNumber evidence="4 14">3.1.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">SSO1392</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="14">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="14">Mg(2+) or Mn(2+) required for ssDNA cleavage activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the CRISPR-associated exonuclease Cas4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.12.1" evidence="4 14"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90296">
        <property type="entry name" value="E90296"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YD3"/>
    <dbReference type="STRING" id="273057.SSO1392"/>
    <dbReference type="PaxDb" id="273057-SSO1392"/>
    <dbReference type="EnsemblBacteria" id="AAK41628">
        <property type="protein sequence ID" value="AAK41628"/>
        <property type="gene ID" value="SSO1392"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1392"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1408"/>
    <dbReference type="eggNOG" id="arCOG00786">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125304_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YD3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045145">
        <property type="term" value="F:single-stranded DNA 5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051827">
        <property type="entry name" value="Cas4_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013343">
        <property type="entry name" value="CRISPR-assoc_prot_Cas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022765">
        <property type="entry name" value="Dna2/Cas4_DUF83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00372">
        <property type="entry name" value="cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531">
        <property type="entry name" value="CRISPR-ASSOCIATED EXONUCLEASE CAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531:SF6">
        <property type="entry name" value="DNA REPLICATION ATP-DEPENDENT HELICASE_NUCLEASE DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01930">
        <property type="entry name" value="Cas_Cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="9 14" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 14" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13 14" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF83" evidence="15">
        <location>
            <begin position="21"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AAK41628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="201" mass="23547" checksum="1F0743981F5CDD4C" modified="2001-10-01" version="1">MRVIACFIRFVTLNVLMTISGITIKHFAYCPQIVRIESMGFTERVSEAMIEGEQVEKDKVMNFLYATLKPLNVVGKPIFRYGDLIGSPDYVLIYPNHWVPLDVKSGRKRYDHKLQMKYYLYLMDMNGINVKEGLLYYVSLKEMVRLEYNYAEKRYVEKVLSKIREAINGKVRVVQDARKCYNCGFFVYCKPKIKGNLAYVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YH6</accession>
    <name>Q97YH6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1160</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO7758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK67520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YH6"/>
    <dbReference type="PaxDb" id="273057-SSO7758"/>
    <dbReference type="EnsemblBacteria" id="AAK67520">
        <property type="protein sequence ID" value="AAK67520"/>
        <property type="gene ID" value="SSO7758"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7758"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1351"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2968627_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YH6"/>
    <dbReference type="PhylomeDB" id="Q97YH6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK67520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="58" mass="6902" checksum="B50E32EDD7C3C5F3" modified="2001-10-01" version="1">MFKTSSQTVRNYAEKEEVAEKLLKRVKQLSLEMLKAVKKVNISIDWTTIKYQTNREVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YN7</accession>
    <name>Q97YN7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1287</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90283">
        <property type="entry name" value="F90283"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923290.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YN7"/>
    <dbReference type="STRING" id="273057.SSO1287"/>
    <dbReference type="PaxDb" id="273057-SSO1287"/>
    <dbReference type="EnsemblBacteria" id="AAK41525">
        <property type="protein sequence ID" value="AAK41525"/>
        <property type="gene ID" value="SSO1287"/>
    </dbReference>
    <dbReference type="GeneID" id="1454304"/>
    <dbReference type="KEGG" id="sso:SSO1287"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1286"/>
    <dbReference type="eggNOG" id="arCOG00385">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_697597_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YN7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053108">
        <property type="entry name" value="Chlamydial_TARP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36975">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36975:SF5">
        <property type="entry name" value="TRANSLOCATED ACTIN-RECRUITING PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="79"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="403" mass="44748" checksum="4484E0491DD9CD77" modified="2001-10-01" version="1">MKTYLCDTMDVKYILVVIIIITFIVNIVSLFYLNSQIASLSSSYNTLVNNYNTLRTYYQNLNSNYTTLYSSYSNLVNSYNSLSSQYAKLSSEYNTLMAKYDNLTAKYNTLSQNYTILSGQLALTMGTMTVQSFYIYLAQVNTQGMESLLVGPLASYFEITSPPGNGTIIASPANSSDALPLIGSKLSQFFNYLSVKTEVKELVITPLENYVLGEGLVSFNDQYANGTIVTNYALITVVAQEINLSTWQVVYVKINNALTQSQYNTLVTLFNLIQALESKNIGQLQSILVGPYQSYVYIARGPYAGNYSGLDVPNVFIDTIISKDVSSLQFELYYFNITPLNPTTSLVDMYGVLKITLSNGSTYTSYTDLRTTVELEPNGVPQVVALNIINDLTQQQVVSALPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YX7</accession>
    <name>Q97YX7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thermopsin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="H90270"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923221.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YX7"/>
    <dbReference type="STRING" id="273057.SSO1175"/>
    <dbReference type="MEROPS" id="A37.001"/>
    <dbReference type="PaxDb" id="273057-SSO1175"/>
    <dbReference type="EnsemblBacteria" id="AAK41423">
        <property type="protein sequence ID" value="AAK41423"/>
        <property type="gene ID" value="SSO1175"/>
    </dbReference>
    <dbReference type="GeneID" id="1454202"/>
    <dbReference type="KEGG" id="sso:SSO1175"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1168"/>
    <dbReference type="eggNOG" id="arCOG03671">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_443224_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YX7"/>
    <dbReference type="BRENDA" id="3.4.23.42">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="3.4.23.B25">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="577"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="601" mass="65103" checksum="3C9DFFD58B5E2421" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z12</accession>
    <name>Q97Z12_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Heterodisulfide reductase subunit C (HdrC-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hdrC-2</name>
        <name evidence="3" type="ordered locus">SSO1134</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HdrC family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="C90266"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989761.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z12"/>
    <dbReference type="FunCoup" id="Q97Z12">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1134"/>
    <dbReference type="PaxDb" id="273057-SSO1134"/>
    <dbReference type="EnsemblBacteria" id="AAK41386">
        <property type="protein sequence ID" value="AAK41386"/>
        <property type="gene ID" value="SSO1134"/>
    </dbReference>
    <dbReference type="GeneID" id="1454165"/>
    <dbReference type="KEGG" id="sso:SSO1134"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1126"/>
    <dbReference type="eggNOG" id="arCOG00966">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093432_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z12"/>
    <dbReference type="PhylomeDB" id="Q97Z12"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051460">
        <property type="entry name" value="HdrC_iron-sulfur_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255:SF2">
        <property type="entry name" value="HETERODISULFIDE REDUCTASE RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="98"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="230" mass="25671" checksum="37452BD041573672" modified="2001-10-01" version="1">MPIPELEKPIIKGLIQKDKVIVDGVELDGTWNAFMIERTQTGYDPTVWDEIANTLEGVTISACWQCGTCTSGCTMREYDPDYSPRRFIDLARKGDKQALIELQNSLWRCVSCQKCTHRCPKGVLVEEVVHSIHHYLLKHGLVKKDPGTVFDELFLETVIKNGGRISELTLGAAAAKAGMVTLSLKDLINIGAAILKGGLIKDVLRPNRVKNWDKIQKVLEEAMKEEVVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z32</accession>
    <name>Q97Z32_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90263">
        <property type="entry name" value="E90263"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z32"/>
    <dbReference type="STRING" id="273057.SSO1107"/>
    <dbReference type="PaxDb" id="273057-SSO1107"/>
    <dbReference type="EnsemblBacteria" id="AAK41364">
        <property type="protein sequence ID" value="AAK41364"/>
        <property type="gene ID" value="SSO1107"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1107"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1104"/>
    <dbReference type="eggNOG" id="arCOG05918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2079408_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z32"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="116" mass="13701" checksum="0C3F4951DF6DCA92" modified="2001-10-01" version="1">MSIENEAKKLAATYARWLRNPQDALFGKDGEGVVLKIYKKIKQAKDKNEIIEILRLDQYTMEKTTFNDMTRFVNDLLNKIQQMDDQLALRFTVEVFRYFQIALATKMEDMNKGLWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z56</accession>
    <name>Q97Z56_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter, ATP binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1078</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90260">
        <property type="entry name" value="E90260"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923191.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z56"/>
    <dbReference type="FunCoup" id="Q97Z56">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1078"/>
    <dbReference type="PaxDb" id="273057-SSO1078"/>
    <dbReference type="EnsemblBacteria" id="AAK41340">
        <property type="protein sequence ID" value="AAK41340"/>
        <property type="gene ID" value="SSO1078"/>
    </dbReference>
    <dbReference type="GeneID" id="1454119"/>
    <dbReference type="KEGG" id="sso:SSO1078"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1078"/>
    <dbReference type="eggNOG" id="arCOG00922">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="InParanoid" id="Q97Z56"/>
    <dbReference type="PhylomeDB" id="Q97Z56"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098796">
        <property type="term" value="C:membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000032">
        <property type="entry name" value="Export ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015854">
        <property type="entry name" value="ABC_transpr_LolD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24220:SF86">
        <property type="entry name" value="ABC TRANSPORTER ABCH.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24220">
        <property type="entry name" value="IMPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="225" mass="25064" checksum="95F9151FEDEE4628" modified="2001-10-01" version="1">MSVIRLENVTKIYEGNVKTIALRDINLSIDEGEFISILGPSGSGKSTLLSILGILDRPSQGKVYIYEVDVTKLSDNEISKIRNEYIGFVFQNFNLIQRLSVLQNVELPLVARGIPKKKREEIAINSLKLVGLDGLINKKPSELSGGQQQRVAIARALAQNPKIILADEPTGNLDSSNAKIVMNIFKKINEEFKTTIAVVTHDREVASYTKRKIYIRDGKIVGEER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-11" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZC4</accession>
    <name>NADA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Quinolinate synthase</fullName>
            <ecNumber evidence="1">2.5.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nadA</name>
        <name type="ordered locus">SSO0998</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>iminosuccinate + dihydroxyacetone phosphate = quinolinate + phosphate + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25888"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="2.5.1.72"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:25889"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the quinolinate synthase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.72" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90251">
        <property type="entry name" value="F90251"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989240.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZC4"/>
    <dbReference type="SMR" id="Q97ZC4"/>
    <dbReference type="FunCoup" id="Q97ZC4">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0998"/>
    <dbReference type="PaxDb" id="273057-SSO0998"/>
    <dbReference type="DNASU" id="1455236"/>
    <dbReference type="EnsemblBacteria" id="AAK41269">
        <property type="protein sequence ID" value="AAK41269"/>
        <property type="gene ID" value="SSO0998"/>
    </dbReference>
    <dbReference type="GeneID" id="1455236"/>
    <dbReference type="GeneID" id="44129929"/>
    <dbReference type="KEGG" id="sso:SSO0998"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.995"/>
    <dbReference type="eggNOG" id="arCOG04459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047382_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZC4"/>
    <dbReference type="PhylomeDB" id="Q97ZC4"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008987">
        <property type="term" value="F:quinolinate synthetase A activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034628">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-aspartate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10800:FF:000001">
        <property type="entry name" value="Quinolinate synthase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10800">
        <property type="entry name" value="NadA-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00568">
        <property type="entry name" value="NadA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003473">
        <property type="entry name" value="NadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036094">
        <property type="entry name" value="NadA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023066">
        <property type="entry name" value="Quinolinate_synth_type2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00550">
        <property type="entry name" value="nadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006878">
        <property type="entry name" value="PRK09375.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573:SF0">
        <property type="entry name" value="QUINOLINATE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573">
        <property type="entry name" value="QUINOLINATE SYNTHETASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02445">
        <property type="entry name" value="NadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142754">
        <property type="entry name" value="NadA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155812" description="Quinolinate synthase">
        <location>
            <begin position="1"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="113"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="201"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00568"/>
        </source>
    </evidence>
    <sequence length="311" mass="34885" checksum="93515D3B89F3ACD9" modified="2001-10-01" version="1">MTRVEELLSQIKNLKTEKNAIILGHNYMEYSVQLVSDFTGDSYDLAVKAMKTNAKIILFAGVYFMAEQASALNPNKKVLSPDPNAGCTLSDSLDVKTLQEYKEKYPNAPVVLYINTSIYAKALADYIVTSSTAVKVVQKLNADTILFGPDANLANYVQQKVPNKKIIKVPPNGRCIVHANYTKQLVELARKKYPNALLMAHPEAPLEILESADFVGSTNQMIQFSKENKNEEFIVATEIGMINALKIKNPSKKFYPLVTTEACACARCPYMNMINLEKVKRSLEEEVYEVKVPEDIAERVKRAFENTMKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZD9</accession>
    <name>Q97ZD9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribose 5-phosphate isomerase A</fullName>
            <ecNumber evidence="2">5.3.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpiA</name>
        <name evidence="3" type="ordered locus">SSO0978</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.3.1.6" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90249">
        <property type="entry name" value="F90249"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992432.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZD9"/>
    <dbReference type="FunCoup" id="Q97ZD9">
        <property type="interactions" value="378"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0978"/>
    <dbReference type="PaxDb" id="273057-SSO0978"/>
    <dbReference type="EnsemblBacteria" id="AAK41253">
        <property type="protein sequence ID" value="AAK41253"/>
        <property type="gene ID" value="SSO0978"/>
    </dbReference>
    <dbReference type="GeneID" id="34442664"/>
    <dbReference type="KEGG" id="sso:SSO0978"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.978"/>
    <dbReference type="eggNOG" id="arCOG01122">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056590_1_0_2"/>
    <dbReference type="InParanoid" id="Q97ZD9"/>
    <dbReference type="PhylomeDB" id="Q97ZD9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006014">
        <property type="term" value="P:D-ribose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934:SF0">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75445">
        <property type="entry name" value="D-ribose-5-phosphate isomerase (RpiA), lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="NCBIfam" id="TIGR00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="227" mass="25162" checksum="39E6EA467390183B" modified="2001-10-01" version="1">MDPKELLATYTLNYLRGKKIIGVGTGKTVKKLIEVLSSEENLKESSLFVASSIDTEIELSKRGFKVLSLFSAIQPEIYVDSFDIVTKDGIMIKGGGGALLREKLLAYFSKYRIFIGESTKLKDTKVIDIPIEVISVGVSYVKYKLEKMGFTVKIREGSGKMGPIISDNGNVILDVSVKTENLCEFDRMIKNIPSIVETGIFCKELYNKIILANEQGRIEEMDAGDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZI3</accession>
    <name>Q97ZI3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SpoVT-AbrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90243">
        <property type="entry name" value="F90243"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992357.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZI3"/>
    <dbReference type="STRING" id="273057.SSO0923"/>
    <dbReference type="PaxDb" id="273057-SSO0923"/>
    <dbReference type="EnsemblBacteria" id="AAK41205">
        <property type="protein sequence ID" value="AAK41205"/>
        <property type="gene ID" value="SSO0923"/>
    </dbReference>
    <dbReference type="GeneID" id="1455172"/>
    <dbReference type="KEGG" id="sso:SSO0923"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.924"/>
    <dbReference type="eggNOG" id="arCOG00318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069302_1_0_2"/>
    <dbReference type="InParanoid" id="Q97ZI3"/>
    <dbReference type="PhylomeDB" id="Q97ZI3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF5">
        <property type="entry name" value="PHOSPHATE UPTAKE REGULATOR, PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="17"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="333" mass="38103" checksum="18C16E6764854EE3" modified="2001-10-01" version="1">MSEEKIARDIETRKVQKLGSSSLFITLPKKWINKWSIKPGDKVILEALDDGSLRLIAEKAKTNAGRKTILVDVDSLKQPLINIIPCLYILGFDEIVFETKKDFNKKDFEEVLYISKHLVGAEVVESSEKKIKLECLLDTEKVGVESLLRRILNIISKRIDELSKFLTNSHTIHQSGISNSDDLRKIYLMLLRRIIGNKYQSSSTEYYRNSLILLNISILINIDYIVSKIYQIKDNFEVSPSQTDMLKSIFTSINDVLDEIVMSALFPSVKRISNGFNLILQIKQNLLKIKDSLPNSFNLYLEDLVNLLENALNNSMCGIFLEDLPWIEKDLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZL6</accession>
    <name>Q97ZL6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11 12">Threonine synthase</fullName>
            <ecNumber evidence="4 11 12">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">thrC-2</name>
        <name evidence="16" type="ordered locus">SSO0878</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>O-phospho-L-homoserine + H2O = L-threonine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.2.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="4 11 12"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90238">
        <property type="entry name" value="E90238"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992295.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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        <ligand>
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        <source>
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    <sequence length="396" mass="42689" checksum="FF802F3E042DEBDE" modified="2001-10-01" version="1">MKCLNCGYETEIDQSQILCPRCGGLLEIILEPPKDFSFSKLRGRGVWRYKELIAGRYKNIVSINEGNTPLIRSSNINDNLYFKFEGLNPTGSFKDRGMTVAVSSAVSLNYKTVIAASTGNTAASAAAYAARAGIKSFIVLPKGKVALGKLAQSILYGSVILEVDGSFDVAMDAVMRLYKDLKVVYPLNSFNPWRLEGQKTIAFEIAEELGVPDNVIVPVGNAGNIYAIWKGFNELVKTGVINTIPRMIGIQAEGASPIVTAIIKGKDSPDFIENPDTVATAIRIGKPVNWQKAMKAIRESNGTAIAVSDSEILDAQKTLARKEGIGAEPASAAALAGYIKAINEKVVDKDEKTVLILTGHSLKDPDSMTKAEAKRILVNPLHMEKIILGEINGSNS</sequence>
</entry>
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            <fullName evidence="2">Fourth ORF in transposon ISC1225</fullName>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
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            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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    </dbReference>
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        </location>
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        </source>
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        <source>
            <dbReference type="EMBL" id="AAK67513.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
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</entry>
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    <accession>Q97ZR0</accession>
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    </organism>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2010" name="J. Struct. Funct. Genomics" volume="11" first="167" last="180">
            <title>The Scottish Structural Proteomics Facility: targets, methods and outputs.</title>
            <authorList>
                <person name="Oke M."/>
                <person name="Carter L.G."/>
                <person name="Johnson K.A."/>
                <person name="Liu H."/>
                <person name="McMahon S.A."/>
                <person name="Yan X."/>
                <person name="Kerou M."/>
                <person name="Weikart N.D."/>
                <person name="Kadi N."/>
                <person name="Sheikh M.A."/>
                <person name="Schmelz S."/>
                <person name="Dorward M."/>
                <person name="Zawadzki M."/>
                <person name="Cozens C."/>
                <person name="Falconer H."/>
                <person name="Powers H."/>
                <person name="Overton I.M."/>
                <person name="van Niekerk C.A."/>
                <person name="Peng X."/>
                <person name="Patel P."/>
                <person name="Garrett R.A."/>
                <person name="Prangishvili D."/>
                <person name="Botting C.H."/>
                <person name="Coote P.J."/>
                <person name="Dryden D.T."/>
                <person name="Barton G.J."/>
                <person name="Schwarz-Linek U."/>
                <person name="Challis G.L."/>
                <person name="Taylor G.L."/>
                <person name="White M.F."/>
                <person name="Naismith J.H."/>
            </authorList>
            <dbReference type="PubMed" id="20419351"/>
            <dbReference type="DOI" id="10.1007/s10969-010-9090-y"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS)</scope>
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        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H=1-95"/>
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    <dbReference type="SMR" id="Q97ZR0"/>
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    <dbReference type="EnsemblBacteria" id="AAK40899">
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        <property type="gene ID" value="SSO6206"/>
    </dbReference>
    <dbReference type="GeneID" id="65564291"/>
    <dbReference type="KEGG" id="sso:SSO6206"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.595"/>
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    <dbReference type="EvolutionaryTrace" id="Q97ZR0"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1340">
        <property type="entry name" value="MTH889-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003831">
        <property type="entry name" value="DUF211"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023129">
        <property type="entry name" value="MTH889-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42240">
        <property type="entry name" value="DUF211 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42240:SF1">
        <property type="entry name" value="DUF211 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02680">
        <property type="entry name" value="DUF211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160363">
        <property type="entry name" value="MTH889-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="3" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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            <dbReference type="EMBL" id="AAK40899.1"/>
        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2X3D"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="10483" checksum="745AD57124C96046" modified="2001-10-01" version="1">MAIRRLVLDVLKPIRGTSIVDLAERISKLDGVEGVNISVTDMDVETMGLMIIIEGTSLNFDDIRKMLEEEGCAIHSIDEVVSGNRIIEGKIKDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZR2</accession>
    <name>Q97ZR2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATP synthase subunit G (AtpG)</fullName>
            <ecNumber evidence="1">3.6.3.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpG</name>
        <name evidence="1" type="ordered locus">SSO6175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.14" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90203">
        <property type="entry name" value="C90203"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991079.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZR2"/>
    <dbReference type="STRING" id="273057.SSO6175"/>
    <dbReference type="PaxDb" id="273057-SSO6175"/>
    <dbReference type="EnsemblBacteria" id="AAK40882">
        <property type="protein sequence ID" value="AAK40882"/>
        <property type="gene ID" value="SSO6175"/>
    </dbReference>
    <dbReference type="GeneID" id="1454849"/>
    <dbReference type="KEGG" id="sso:SSO6175"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.575"/>
    <dbReference type="eggNOG" id="arCOG07285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3075538_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZR2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012508">
        <property type="entry name" value="ATPase_A1-cplx_e-su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08112">
        <property type="entry name" value="ATP-synt_E_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="52" mass="6336" checksum="E6D7B89FD532EEAA" modified="2001-10-01" version="1">MDQDKYLQILRNSLEEKKSEILKNINMEYEKLLKNRLSQLDEVKRKVLKELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZS4</accession>
    <name>Q97ZS4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO5983</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90196">
        <property type="entry name" value="B90196"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZS4"/>
    <dbReference type="STRING" id="273057.SSO5983"/>
    <dbReference type="PaxDb" id="273057-SSO5983"/>
    <dbReference type="EnsemblBacteria" id="AAK40825">
        <property type="protein sequence ID" value="AAK40825"/>
        <property type="gene ID" value="SSO5983"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO5983"/>
    <dbReference type="HOGENOM" id="CLU_3178767_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZS4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="46" mass="5697" checksum="7184F3E2CCB8D21A" modified="2001-10-01" version="1">MIVTRNLYNFFNWHKNVLVKEGLNILSNSKKRQYLKLLRTIKYIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZV9</accession>
    <name>Q97ZV9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Integral membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO0466</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. MmpL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90191">
        <property type="entry name" value="F90191"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922983.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZV9"/>
    <dbReference type="STRING" id="273057.SSO0466"/>
    <dbReference type="PaxDb" id="273057-SSO0466"/>
    <dbReference type="EnsemblBacteria" id="AAK40789">
        <property type="protein sequence ID" value="AAK40789"/>
        <property type="gene ID" value="SSO0466"/>
    </dbReference>
    <dbReference type="GeneID" id="7807416"/>
    <dbReference type="KEGG" id="sso:SSO0466"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.461"/>
    <dbReference type="eggNOG" id="arCOG02175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_345038_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZV9"/>
    <dbReference type="PhylomeDB" id="Q97ZV9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050545">
        <property type="entry name" value="Mycobact_MmpL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33406">
        <property type="entry name" value="MEMBRANE PROTEIN MJ1562-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33406:SF6">
        <property type="entry name" value="MEMBRANE PROTEIN YDGH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="377"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="452"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="512"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="666"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="693"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="720"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="766"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="798"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane transport protein MMPL" evidence="8">
        <location>
            <begin position="287"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane transport protein MMPL" evidence="8">
        <location>
            <begin position="669"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK40789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="837" mass="95022" checksum="324E85258B243A2C" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZW2</accession>
    <name>Q97ZW2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S54 rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO0463</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90191">
        <property type="entry name" value="C90191"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922980.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZW2"/>
    <dbReference type="STRING" id="273057.SSO0463"/>
    <dbReference type="PaxDb" id="273057-SSO0463"/>
    <dbReference type="EnsemblBacteria" id="AAK40786">
        <property type="protein sequence ID" value="AAK40786"/>
        <property type="gene ID" value="SSO0463"/>
    </dbReference>
    <dbReference type="GeneID" id="1455596"/>
    <dbReference type="KEGG" id="sso:SSO0463"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.457"/>
    <dbReference type="eggNOG" id="arCOG01768">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1529264_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZW2"/>
    <dbReference type="PhylomeDB" id="Q97ZW2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066:SF11">
        <property type="entry name" value="PEPTIDASE S54 RHOMBOID DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="6">
        <location>
            <begin position="74"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="214" mass="23905" checksum="1AB8D9052C00D887" modified="2001-10-01" version="1">MEHDSVGSIDNHCIRTNISIMVNKVIIKKIIGISTFFLMFLVTLGFMVGLLATFIDSTSLYYLEQLNYLVIKGYYSELFTSIFITNSFVDFIFNFISLYVIYLIFGSRAGKHEYGIFILAGILGNLLTVIFYSPFTLSSGASGGIFGLLSYYTFYDFLKKDNLGVYGLVFLVSVFGVSDLIFPNVNVVAHIGGILGGIMYAVVYYLIRSGRTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZW9</accession>
    <name>Q97ZW9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CRISPR-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0454</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90190">
        <property type="entry name" value="D90190"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988704.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZW9"/>
    <dbReference type="SMR" id="Q97ZW9"/>
    <dbReference type="STRING" id="273057.SSO0454"/>
    <dbReference type="PaxDb" id="273057-SSO0454"/>
    <dbReference type="EnsemblBacteria" id="AAK40779">
        <property type="protein sequence ID" value="AAK40779"/>
        <property type="gene ID" value="SSO0454"/>
    </dbReference>
    <dbReference type="GeneID" id="1455589"/>
    <dbReference type="KEGG" id="sso:SSO0454"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.448"/>
    <dbReference type="eggNOG" id="arCOG03358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131316_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZW9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045745">
        <property type="entry name" value="HTH_58_Actinobacteria-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025246">
        <property type="entry name" value="IS30-like_HTH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040595">
        <property type="entry name" value="HTH_Cbp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13936">
        <property type="entry name" value="HTH_38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19575">
        <property type="entry name" value="HTH_58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helix-turn-helix" evidence="2">
        <location>
            <begin position="8"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS30-like HTH" evidence="1">
        <location>
            <begin position="93"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="17578" checksum="E14E9250440E56B6" modified="2001-10-01" version="1">MSEEENIEKVKKMYEEGLSIREIANQLGLSYSKVRRMLIKAKVNFRGKVPNDKIQKIIEMGKQGYSANRISRELNINFNTVLRILKKYNLGKKRRKLDAKEIEKIREEYIKGNSIYRIAKELNISTNLVVYHLKKMGLYRPIRESSPTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZX1</accession>
    <name>Q97ZX1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0451</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90190">
        <property type="entry name" value="B90190"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZX1"/>
    <dbReference type="FunCoup" id="Q97ZX1">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0451"/>
    <dbReference type="PaxDb" id="273057-SSO0451"/>
    <dbReference type="EnsemblBacteria" id="AAK40777">
        <property type="protein sequence ID" value="AAK40777"/>
        <property type="gene ID" value="SSO0451"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0451"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.446"/>
    <dbReference type="eggNOG" id="arCOG00452">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095961_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZX1"/>
    <dbReference type="PhylomeDB" id="Q97ZX1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053492">
        <property type="entry name" value="Cell_Div_Machinery_Comp"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041005">
        <property type="entry name" value="cell_div_CdvB1_B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="284" mass="31916" checksum="A76ACA95F3A521EA" modified="2001-10-01" version="1">MFPSVFIFTLLYSSSFHLRSKYITTLSVDTKMSYSGSSSSLMNVFGILLISDSNNLNSSHYVFDLIRVDEVRYILAMLGKDFQKMWGYEENKFKIKGSKEPLKYKLVQAHYKISSMISRLDAYISRMQERDKILFERVVEAQMSKDTQRAAMYANEVAEIRKISRQLLTTQIALEQVQLRLETVTELGDVFVNLIPVLGVIGELKSALRGIMPEVSLELTELGEGLQEIVTESGEFTGMGSYAAASSPEARKILEEASVVAEQRMKEKFPDLPVGTGLASKSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="144" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZX9</accession>
    <name>Q97ZX9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glycine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">glyS</name>
        <name evidence="10" type="ordered locus">SSO0444</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.1.1.14" evidence="1"/>
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        <property type="entry name" value="A99189"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="GeneID" id="1455578"/>
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    <dbReference type="PATRIC" id="fig|273057.12.peg.436"/>
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        <property type="term" value="F:glycine-tRNA ligase activity"/>
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        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
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        <property type="term" value="P:small molecule metabolic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
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    </dbReference>
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        <property type="entry name" value="aa-tRNA-synt_IIb"/>
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        <property type="entry name" value="aa-tRNA-synth_II"/>
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        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
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    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
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    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
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    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
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    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
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    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
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    </dbReference>
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        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
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    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="9">
        <location>
            <begin position="6"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK40768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="574" mass="66539" checksum="29038309DB868F86" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q980D8</accession>
    <name>Q980D8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Na(+)/H(+) antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">SSO0376</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="AAK40705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="B90181"/>
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        <property type="gene ID" value="SSO0376"/>
    </dbReference>
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    <dbReference type="KEGG" id="sso:SSO0376"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.369"/>
    <dbReference type="eggNOG" id="arCOG01956">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_541436_0_0_2"/>
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        <property type="component" value="Chromosome"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cation/H_exchanger_TM"/>
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        <property type="entry name" value="CBS_dom_sf"/>
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    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562">
        <property type="entry name" value="NAPA-TYPE SODIUM/HYDROGEN ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562:SF3">
        <property type="entry name" value="SODIUM ION_PROTON EXCHANGER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="10" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="386"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAK40705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="505" mass="56671" checksum="2A28DA285D3415CA" modified="2001-10-01" version="1">MVSLLLISLLYLGIMLIAAKISEEIFRRIGLVTFVGPILMGILLGNGVFGVIQINEIISFITSLGIVFLLFLAGAEEFNVENRPNANVFLAVILESIIPFSVILVAIYIIGFKNYLILIVPLAMSSAGPLSRMLMDVKISDNRISNLIFQQVILIEIIFVILFAILLKTSEILITIIEIFLVFAFTIMFGRTLAKILEKIEYYFKAREIEFASLIAIILIIGYLAELYKFNSAISAFFLGILLKDYLKDRPELLERLHAFTYGFFEPLFFLGIGLYVTKINASIILFSLLFFSLIIASKFLAGFISSKILNIKGKINGFATSVKGGVDSSLLLTGLTLGYINEFMYSFSILAISLSALIVPLLFQINFKISNKISESKKVKLSQEVIKLQIKPLYASCEENLRSVISKISERGVRGIVVVNYENKPLGYVSIQTLLEIDPELYESTKACEVPLDEVPIEYDNVKVIDLLRKFRETEKPVIAIINKEGVLVTTVYERELARFLISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q980E6</accession>
    <name>Q980E6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Molybdate/tungstate import ATP-binding protein WtpC</fullName>
            <ecNumber evidence="6">7.3.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO0367</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tungstate(in) + ATP + H2O = tungstate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35027"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">The complex is composed of two ATP-binding proteins (WtpC), two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.6" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90180">
        <property type="entry name" value="B90180"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990669.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980E6"/>
    <dbReference type="FunCoup" id="Q980E6">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0367"/>
    <dbReference type="PaxDb" id="273057-SSO0367"/>
    <dbReference type="EnsemblBacteria" id="AAK40697">
        <property type="protein sequence ID" value="AAK40697"/>
        <property type="gene ID" value="SSO0367"/>
    </dbReference>
    <dbReference type="GeneID" id="1455507"/>
    <dbReference type="KEGG" id="sso:SSO0367"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.361"/>
    <dbReference type="eggNOG" id="arCOG05705">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="InParanoid" id="Q980E6"/>
    <dbReference type="PhylomeDB" id="Q980E6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901238">
        <property type="term" value="F:ABC-type tungstate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050093">
        <property type="entry name" value="ABC_SmlMolc_Importer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781">
        <property type="entry name" value="SPERMIDINE/PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781:SF4">
        <property type="entry name" value="SPERMIDINE_PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK40697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="202" mass="22685" checksum="0AA6D0473EB8BA66" modified="2001-10-01" version="1">MIEVYVKKKLQAFLLNVNFSEKGIIAITGKNGSGKSTLLNVIAGILKPDEGYIRLNDIDITNLPINKRNVILVTPDSYIPTLDLKKHLTWGAKLRGRKVNDKEVMEVAKLLGVPNENKKVGNLSLGNREKVSLATAILTKPNLILVDEGFSNISEKEYFINIYTDLCKKNQIDLIYVTQDTEDAKFSDHHYVMNNGSLHKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q980F5</accession>
    <name>Q980F5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphate regulatory protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90178">
        <property type="entry name" value="H90178"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990653.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980F5"/>
    <dbReference type="STRING" id="273057.SSO0356"/>
    <dbReference type="PaxDb" id="273057-SSO0356"/>
    <dbReference type="EnsemblBacteria" id="AAK40687">
        <property type="protein sequence ID" value="AAK40687"/>
        <property type="gene ID" value="SSO0356"/>
    </dbReference>
    <dbReference type="GeneID" id="1455497"/>
    <dbReference type="KEGG" id="sso:SSO0356"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.348"/>
    <dbReference type="eggNOG" id="arCOG00318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069302_1_0_2"/>
    <dbReference type="InParanoid" id="Q980F5"/>
    <dbReference type="PhylomeDB" id="Q980F5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF2">
        <property type="entry name" value="PHOU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="8"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="351" mass="40385" checksum="A0AB316697E1EA2F" modified="2001-10-01" version="1">MEVRRVQKFGKSTLMVSLPAEWVKEVSLSPGESVYLEVDEDGSLKVYPPNLKAESKVKEMKISIQNDSVQGELVSRVIYSLYILGYDRIDIESKSGIFTEDILRKVKDTIRNLIGLEIVSQTTDTIQIQSFLDPTKYTMNNLINRLSNSIKQMLHYLDLGIKESSRTFLQEVIELEREVDRLYYLALRQLLLAQMNRSLAYMIGVKRIQIVGNRILIKAIEEAADEISEIALDLLSLHPSDLEEMKRLWNKINMYIEQTSSVIDHAVKVLAKEDTILINEVMEELRTLRRVLITEAIISEEMLKEVKSPSLIAVLRALNLRLYNAIRRMEPITEIAFNRSIENQKEIIITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q980K1</accession>
    <name>Q980K1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA-directed RNA polymerase, putative subunit M (RpoM-like)</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoM-like</name>
        <name evidence="1" type="ordered locus">SSO5576</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90171">
        <property type="entry name" value="G90171"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990576.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980K1"/>
    <dbReference type="SMR" id="Q980K1"/>
    <dbReference type="STRING" id="273057.SSO5576"/>
    <dbReference type="PaxDb" id="273057-SSO5576"/>
    <dbReference type="EnsemblBacteria" id="AAK40630">
        <property type="protein sequence ID" value="AAK40630"/>
        <property type="gene ID" value="SSO5576"/>
    </dbReference>
    <dbReference type="GeneID" id="1455440"/>
    <dbReference type="KEGG" id="sso:SSO5576"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.285"/>
    <dbReference type="eggNOG" id="arCOG07176">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2434004_0_0_2"/>
    <dbReference type="InParanoid" id="Q980K1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="90" mass="10446" checksum="CD8B7445F9A20715" modified="2001-10-01" version="1">MKKETRIAVFVKVNYLYAICVFRGNFLEKMFLDINQDNLIKQITTSSIVDEIKYSNIGIGENFKEQVLEKICENLIKKLSEKLNIDKVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q980R8</accession>
    <name>Q980R8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Triphosphoribosyl-dephospho-CoA protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0212</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90162">
        <property type="entry name" value="D90162"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980R8"/>
    <dbReference type="FunCoup" id="Q980R8">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0212"/>
    <dbReference type="PaxDb" id="273057-SSO0212"/>
    <dbReference type="EnsemblBacteria" id="AAK40555">
        <property type="protein sequence ID" value="AAK40555"/>
        <property type="gene ID" value="SSO0212"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0212"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.210"/>
    <dbReference type="eggNOG" id="arCOG04238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063627_0_0_2"/>
    <dbReference type="InParanoid" id="Q980R8"/>
    <dbReference type="PhylomeDB" id="Q980R8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046917">
        <property type="term" value="F:triphosphoribosyl-dephospho-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4200.10">
        <property type="entry name" value="Triphosphoribosyl-dephospho-CoA protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002736">
        <property type="entry name" value="CitG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42280">
        <property type="entry name" value="CITG FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42280:SF1">
        <property type="entry name" value="CITG FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01874">
        <property type="entry name" value="CitG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="33942" checksum="951D204A729182BD" modified="2001-10-01" version="1">MTKMLEGLLDLCNDVSYILSQASIIESYIFKPGNASRSQDLVNVKYIDIVKSALISSTYYKELCIRGFRGISRIYDTLVELVTRSNKFGFEYQLFGTYLLIAPFAYEAAKINNVFELRKKAAKLLRSLGALETRWFLDALKELNLKYLGKLSSMDYREIENIDFFTLMEFSSKYDIVALNMVNDYSITFAAYKVIKEGLCGLERDVQRAFLYILSNYPDTLIYKKYGAHAALEASKVARLVFSGDCPSSNELEWLNKYLINNNFNPGSTADLIASALAIYYLDEWYGKKGNPHSGFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q980W1</accession>
    <name>Q980W1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UDP-glucose 4-epimerase (GalE-2)</fullName>
            <ecNumber evidence="3">5.1.3.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">galE-2</name>
        <name evidence="3" type="ordered locus">SSO0166</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.2" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90156">
        <property type="entry name" value="H90156"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990390.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980W1"/>
    <dbReference type="FunCoup" id="Q980W1">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0166"/>
    <dbReference type="PaxDb" id="273057-SSO0166"/>
    <dbReference type="EnsemblBacteria" id="AAK40511">
        <property type="protein sequence ID" value="AAK40511"/>
        <property type="gene ID" value="SSO0166"/>
    </dbReference>
    <dbReference type="GeneID" id="1453338"/>
    <dbReference type="KEGG" id="sso:SSO0166"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.162"/>
    <dbReference type="eggNOG" id="arCOG01369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_2"/>
    <dbReference type="InParanoid" id="Q980W1"/>
    <dbReference type="PhylomeDB" id="Q980W1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01713">
        <property type="entry name" value="NUCEPIMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="311" mass="35423" checksum="A26AC1FE5930051B" modified="2001-10-01" version="1">MYIVTGGAGYIGGHLVDYLISKNLEVIVIDDLSYGKYRNEKAKFVMFDLRQNMGELVEKLEKNPIIYHLAANPDVRTSMINVEEHFERDVKVTLNVMELARRVDAEKVIFTSSSTVYGETSKIPTPESEELKPISNYGLFKLLCENIVKYYAEQYGIKSISTRLANITGGRVSHGVVIDFIKKLKDNPNLLEILGNGKQRKSYLYIDDLIEAFVMLEKKVNRIYDVFNIGNNDSITVDEIAKIVIDEMKLSPRITYKNPTADGRGWPGDVRLMLLDISKISREVGWSPKMSSREVIRQAVKDLLYGYKSVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q981A1</accession>
    <name>Q981A1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90144">
        <property type="entry name" value="F90144"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988847.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981A1"/>
    <dbReference type="STRING" id="273057.SSO0048"/>
    <dbReference type="PaxDb" id="273057-SSO0048"/>
    <dbReference type="EnsemblBacteria" id="AAK40413">
        <property type="protein sequence ID" value="AAK40413"/>
        <property type="gene ID" value="SSO0048"/>
    </dbReference>
    <dbReference type="GeneID" id="1455304"/>
    <dbReference type="KEGG" id="sso:SSO0048"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.51"/>
    <dbReference type="eggNOG" id="arCOG02242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150444_0_0_2"/>
    <dbReference type="InParanoid" id="Q981A1"/>
    <dbReference type="PhylomeDB" id="Q981A1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053795">
        <property type="entry name" value="Lrs14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040939">
        <property type="entry name" value="trans_reg_lrs14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="26"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="129" mass="14329" checksum="6A29236CE023316A" modified="2001-10-01" version="1">MSETQLSEGTRIKLPSGKDAGLVDILSFCYGLSETDVTVLLALMKGDARGTEELESDLKLSKASINRSLNKLLEMGLVMRIKEPGNKAGRPRYLYKARDYAELKGKMLSDIKDCADKMAQLVEKEFKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q981C7</accession>
    <name>Q981C7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
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            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
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</entry>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Cell Res." volume="26" first="1288" last="1301">
            <title>Crystal structures of the TRIC trimeric intracellular cation channel orthologues.</title>
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                <person name="Kasuya G."/>
                <person name="Hiraizumi M."/>
                <person name="Maturana A.D."/>
                <person name="Kumazaki K."/>
                <person name="Fujiwara Y."/>
                <person name="Liu K."/>
                <person name="Nakada-Nakura Y."/>
                <person name="Iwata S."/>
                <person name="Tsukada K."/>
                <person name="Komori T."/>
                <person name="Uemura S."/>
                <person name="Goto Y."/>
                <person name="Nakane T."/>
                <person name="Takemoto M."/>
                <person name="Kato H.E."/>
                <person name="Yamashita K."/>
                <person name="Wada M."/>
                <person name="Ito K."/>
                <person name="Ishitani R."/>
                <person name="Hattori M."/>
                <person name="Nureki O."/>
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            <dbReference type="PubMed" id="27909292"/>
            <dbReference type="DOI" id="10.1038/cr.2016.140"/>
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        <scope>X-RAY CRYSTALLOGRAPHY (2.64 ANGSTROMS) OF 1-198</scope>
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    <reference evidence="11" key="3">
        <citation type="journal article" date="2017" name="BMC Biol." volume="0" first="0" last="0">
            <title>Ion and water binding sites inside an occluded hourglass pore of a TRIC channel.</title>
            <authorList>
                <person name="Ou X.M."/>
                <person name="Guo J.L."/>
                <person name="Wang L.F."/>
                <person name="Yang H.T."/>
                <person name="Liu X.Y."/>
                <person name="Sun J.Y."/>
                <person name="Liu Z.F."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS)</scope>
    </reference>
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        <subcellularLocation>
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        <property type="entry name" value="C90140"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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        <property type="chains" value="C/D/E=1-198"/>
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        <property type="entry name" value="UPF0126 INNER MEMBRANE PROTEIN YADS-RELATED"/>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
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                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
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                <person name="Heikamp-de Jong I."/>
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        <property type="entry name" value="His_deacetylse_dom_sf"/>
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    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40374.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
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    </evidence>
    <sequence length="327" mass="37091" checksum="F56040CF42A88468" modified="2001-10-01" version="1">MITIVYDDIYKYHAPKGYHVENPSRIDKALSAINQLKVKFKKPIKVDDPQIIHSEDYVKLVEKHSKLEENLDEDTYTNRYTYESALYAMGGALEAFETNGFALVRPPGHHAGVNGRAFGAPTLGFCIFNNVAYPIKKYKLKRVAIIDFDVHYGNGTQEIFYDDPDILHIDVHQDPRTIYPGNGFPDMIGEKDAEGTKINLLIPPLGSDDLYEELIPIIQAILDDFKPAVIAYSAGFDSYMGDGLASVNATEYTFYSFGVLSNKFTKKYAVLEGGYDKGLIKGLKAFLEGFANIKMEYKKYQSNDSVRSRFMNYLSEEKNILRNYWSI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UWW1</accession>
    <name>PRIL_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA primase large subunit PriL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">priL</name>
        <name type="synonym">priB</name>
        <name type="ordered locus">SSO0557</name>
        <name type="ORF">C21_042</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2004" name="J. Mol. Biol." volume="344" first="1251" last="1263">
            <title>The heterodimeric primase of the hyperthermophilic archaeon Sulfolobus solfataricus possesses DNA and RNA primase, polymerase and 3'-terminal nucleotidyl transferase activities.</title>
            <authorList>
                <person name="Lao-Sirieix S.H."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="15561142"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2004.10.018"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>GENE NAME</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2007" name="Nat. Struct. Mol. Biol." volume="14" first="875" last="877">
            <title>An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis.</title>
            <authorList>
                <person name="Klinge S."/>
                <person name="Hirst J."/>
                <person name="Maman J.D."/>
                <person name="Krude T."/>
                <person name="Pellegrini L."/>
            </authorList>
            <dbReference type="PubMed" id="17704817"/>
            <dbReference type="DOI" id="10.1038/nsmb1288"/>
        </citation>
        <scope>IRON-SULFUR-BINDING</scope>
        <scope>COFACTOR</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2006" name="EMBO Rep." volume="7" first="539" last="545">
            <title>GINS, a central nexus in the archaeal DNA replication fork.</title>
            <authorList>
                <person name="Marinsek N."/>
                <person name="Barry E.R."/>
                <person name="Makarova K.S."/>
                <person name="Dionne I."/>
                <person name="Koonin E.V."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="16485022"/>
            <dbReference type="DOI" id="10.1038/sj.embor.7400649"/>
        </citation>
        <scope>INTERACTION WITH GINS15 AND PRIS</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2005" name="Nat. Struct. Mol. Biol." volume="12" first="1137" last="1144">
            <title>Structure of the heterodimeric core primase.</title>
            <authorList>
                <person name="Lao-Sirieix S.H."/>
                <person name="Nookala R.K."/>
                <person name="Roversi P."/>
                <person name="Bell S.D."/>
                <person name="Pellegrini L."/>
            </authorList>
            <dbReference type="PubMed" id="16273105"/>
            <dbReference type="DOI" id="10.1038/nsmb1013"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.33 ANGSTROMS) OF 1-212</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stabilizes and modulates the activity of the small subunit, increasing the rate of DNA synthesis, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair. Possesses a template-independent 3'-terminal nucleotidyl transferase activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1 5">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3 4">Heterodimer of a small subunit (PriS) and a large subunit (PriL) (PubMed:15561142, PubMed:16485022, PubMed:16273105). Interacts with Gins23 (PubMed:16485022).</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-8081454">
            <id>Q9UWW1</id>
        </interactant>
        <interactant intactId="EBI-8081502">
            <id>Q97Z83</id>
            <label>priS</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>5</experiments>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-8081454">
            <id>Q9UWW1</id>
        </interactant>
        <interactant intactId="EBI-8081318">
            <id>Q9UXG0</id>
            <label>ORF-c40_009</label>
        </interactant>
        <organismsDiffer>true</organismsDiffer>
        <experiments>3</experiments>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic-type primase large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90202">
        <property type="entry name" value="C90202"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991067.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1ZT2">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.33 A"/>
        <property type="chains" value="B/D=1-212"/>
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    <dbReference type="PDB" id="5OF3">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.91 A"/>
        <property type="chains" value="B/E=1-307"/>
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    <dbReference type="PDB" id="5OFN">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="B/C=1-271"/>
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    <dbReference type="PDBsum" id="1ZT2"/>
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    <dbReference type="PDBsum" id="5OFN"/>
    <dbReference type="AlphaFoldDB" id="Q9UWW1"/>
    <dbReference type="SMR" id="Q9UWW1"/>
    <dbReference type="DIP" id="DIP-42888N"/>
    <dbReference type="FunCoup" id="Q9UWW1">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="IntAct" id="Q9UWW1">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="MINT" id="Q9UWW1"/>
    <dbReference type="STRING" id="273057.SSO0557"/>
    <dbReference type="PaxDb" id="273057-SSO0557"/>
    <dbReference type="EnsemblBacteria" id="AAK40874">
        <property type="protein sequence ID" value="AAK40874"/>
        <property type="gene ID" value="SSO0557"/>
    </dbReference>
    <dbReference type="GeneID" id="1454841"/>
    <dbReference type="GeneID" id="44129563"/>
    <dbReference type="KEGG" id="sso:SSO0557"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.567"/>
    <dbReference type="eggNOG" id="arCOG03013">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052778_1_0_2"/>
    <dbReference type="InParanoid" id="Q9UWW1"/>
    <dbReference type="PhylomeDB" id="Q9UWW1"/>
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        <property type="organism ID" value="6163"/>
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    <dbReference type="BRENDA" id="2.7.7.B16">
        <property type="organism ID" value="6163"/>
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    <dbReference type="EvolutionaryTrace" id="Q9UWW1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0051539">
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0003899">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006269">
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="HAMAP" id="MF_00701">
        <property type="entry name" value="DNA_primase_lrg_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA_primase_C"/>
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    <dbReference type="InterPro" id="IPR023642">
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        <property type="match status" value="1"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0639">Primosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000046787" description="DNA primase large subunit PriL">
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    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15561142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="16273105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="16485022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="17704817"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="5OF3"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="5OFN"/>
        </source>
    </evidence>
    <sequence length="307" mass="35734" checksum="5EF09A913B4ABAC3" modified="2000-05-01" version="1">MALDVKKYPFIKSLDDELKKYGGGITLTDLLLNSTTLIDQAKDRIQKTKSGDELPHYVSYNEPVLVFYTTLLSLAILNDVKLIRRYAYAEAKQFRSLLHTENEENLLEISKLLDLKINRCDPIKFYLEKKRRIIQKEFCVHFIDYLKYTKDLKEDWKLSGQILHKGYVYLDKNQLIGLIAESIKSKIVEMIRPLNLKEIPEKLKSLIERRGIIPPCIENILAKEKLNEEEIRTLITFYIDIGKGLSGIVSIMKKYNVSNVEDLYRKYRGDKGTRYIVYSCAKMKQLGLCVSSCNVKNPLQLYFLSNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-01-24" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UWW5</accession>
    <name>AATE_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">A-type ATP synthase subunit E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpE</name>
        <name type="ordered locus">SSO0561</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90202">
        <property type="entry name" value="G90202"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991072.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UWW5"/>
    <dbReference type="SMR" id="Q9UWW5"/>
    <dbReference type="FunCoup" id="Q9UWW5">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0561"/>
    <dbReference type="PaxDb" id="273057-SSO0561"/>
    <dbReference type="EnsemblBacteria" id="AAK40878">
        <property type="protein sequence ID" value="AAK40878"/>
        <property type="gene ID" value="SSO0561"/>
    </dbReference>
    <dbReference type="GeneID" id="1454845"/>
    <dbReference type="KEGG" id="sso:SSO0561"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.571"/>
    <dbReference type="eggNOG" id="arCOG00869">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1412391_0_0_2"/>
    <dbReference type="InParanoid" id="Q9UWW5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="entry name" value="ATP synthase, E subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160527">
        <property type="entry name" value="V-type ATPase subunit E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000117325" description="A-type ATP synthase subunit E">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00311"/>
        </source>
    </evidence>
    <sequence length="194" mass="22633" checksum="057157C921ED8449" modified="2000-05-01" version="1">MDFEQLLDKSLNKVREEIKTELSKSLDEAIKLLNEGHNKIVQEYSQRINELIVKTKEEIEGEKARLEVENKRTLLVEKEYWINKVYERVLGKIGEVVKTKEYKDAIENIISKEIKEIREEKVTIYCSPNDKLMIEKIVGNNKNVTVKTDEKMLGGIKIYFERSGLTRDFSLKLILDQVFDSMRGKISDMLFGGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX16</accession>
    <name>TYW3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.282</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA wyosine derivatives biosynthesis protein Taw3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">taw3</name>
        <name type="ordered locus">SSO0622</name>
        <name type="ORF">C08_028</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="submission" date="2004-06" db="PDB data bank">
            <title>Crystal structure of conserved hypothetical protein SSO0622 from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Jia Z."/>
                <person name="Wong A.H.Y."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wyosine derivatives biosynthesis pathway. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36635"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.1.1.282"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TYW3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.282" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90209">
        <property type="entry name" value="F90209"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991156.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1TLJ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B=1-209"/>
    </dbReference>
    <dbReference type="PDBsum" id="1TLJ"/>
    <dbReference type="AlphaFoldDB" id="Q9UX16"/>
    <dbReference type="SMR" id="Q9UX16"/>
    <dbReference type="FunCoup" id="Q9UX16">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0622"/>
    <dbReference type="PaxDb" id="273057-SSO0622"/>
    <dbReference type="EnsemblBacteria" id="AAK40933">
        <property type="protein sequence ID" value="AAK40933"/>
        <property type="gene ID" value="SSO0622"/>
    </dbReference>
    <dbReference type="GeneID" id="1454900"/>
    <dbReference type="KEGG" id="sso:SSO0622"/>
    <dbReference type="eggNOG" id="arCOG04156">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047426_2_1_2"/>
    <dbReference type="InParanoid" id="Q9UX16"/>
    <dbReference type="PhylomeDB" id="Q9UX16"/>
    <dbReference type="EvolutionaryTrace" id="Q9UX16"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1960.10">
        <property type="entry name" value="tRNA wybutosine-synthesizing-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00266">
        <property type="entry name" value="TYW3_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022908">
        <property type="entry name" value="Taw3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003827">
        <property type="entry name" value="tRNA_yW-synthesising"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036602">
        <property type="entry name" value="tRNA_yW-synthesising-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003263">
        <property type="entry name" value="PRK04235.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02676">
        <property type="entry name" value="TYW3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111278">
        <property type="entry name" value="SSo0622-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000157102" description="tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="3"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="26"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="29"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="40"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="51"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="67"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="78"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="90"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="101"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="128"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="136"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="146"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="161"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="1TLJ"/>
        </source>
    </evidence>
    <sequence length="209" mass="24115" checksum="EA94C8B46E1FB88B" modified="2000-05-01" version="1">MLVWEELREKALNKIYHDKEIGYLDPDILGFLLAFYRNRNDVYTQSSCSGRITIVDAEMPWDRKNSTIIFKNHLRITEQDLEDVLSKNQVRRLWLIVQGPIIHIYAKNIETGWDILKIAREAGFKHSGILATNQKGVLVELRTGIRMVHLLRESNTERVDKDKIKTLVNVCNEVLARGKQKMNLLKDLLSSSSNNSMELGKNSKLKTPI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-04-23" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX31</accession>
    <name>ASSY_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate synthase</fullName>
            <ecNumber evidence="1">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argG</name>
        <name type="ordered locus">SSO0638</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-citrulline + L-aspartate + ATP = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40946" version="1"/>
        </conflict>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAB57663" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57663.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40946.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90211">
        <property type="entry name" value="C90211"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_173645063.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX31"/>
    <dbReference type="SMR" id="Q9UX31"/>
    <dbReference type="FunCoup" id="Q9UX31">
        <property type="interactions" value="270"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0638"/>
    <dbReference type="PaxDb" id="273057-SSO0638"/>
    <dbReference type="EnsemblBacteria" id="AAK40946">
        <property type="protein sequence ID" value="AAK40946"/>
        <property type="gene ID" value="SSO0638"/>
    </dbReference>
    <dbReference type="GeneID" id="1454913"/>
    <dbReference type="KEGG" id="sso:SSO0638"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.643"/>
    <dbReference type="eggNOG" id="arCOG00112">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032784_4_0_2"/>
    <dbReference type="InParanoid" id="Q9UX31"/>
    <dbReference type="PhylomeDB" id="Q9UX31"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000053">
        <property type="term" value="P:argininosuccinate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="ASS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Argininosuccinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Argininosuccinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="entry name" value="Argininosuccinate synthetase, chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00005">
        <property type="entry name" value="Arg_succ_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048268">
        <property type="entry name" value="Arginosuc_syn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048267">
        <property type="entry name" value="Arginosuc_syn_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001770">
        <property type="entry name" value="PRK00509.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587:SF2">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20979">
        <property type="entry name" value="Arginosuc_syn_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="Argininosuccinate synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148686" description="Argininosuccinate synthase">
        <location>
            <begin position="1"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="6"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="391" mass="44487" checksum="58565E5F25A4F5FC" modified="2005-09-27" version="2">MKIVLAYSGGLDTTVSIRWLKETFKAEIITVTVDVGQKDDFKKIEERAYIAGASKHYTIDAVRQFANNYIAYAIKLNGLYEGVYPLSTALARPLIAEKVVEVAKKEGAEAVAHGSTSKGNDQVRFDLAVKALYPDVKIIAPARIWNMTREDEIKYAKEKGIPIKVESDKYSIDENLWGRSIEGDIISDPSLEVPEDAFEWTKQIYNKKEIVSIEFSNGVPTAVNGEKMELNKLVDFLNLKFGSHGFGRVEHIENRVVGFKSREVYEVPAALGLIYAHIDLEKTIYTPMELRFKRHIDQLWSDLVYQGLWFEPLRETLHKVADEMNKWISGEAKVEVSNGSFRIVGRESEYSPYSEKIASYNKGWYPSDEMARGFIEIWGMHSLIARRVRGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX87</accession>
    <name>RS5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein uS5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">30S ribosomal protein S5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Ribosomal protein S5 (HMAS5)</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps5</name>
        <name evidence="5" type="synonym">rps5AB</name>
        <name type="ordered locus">SSO0698</name>
        <name type="ORF">C10_032</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="3">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="function">
        <text evidence="1">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 7 8 9 10 11 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6 7 8 9 10 12">Binds 1 Zn(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Part of the 30S ribosomal subunit (PubMed:39753558). Contacts protein S4 (PubMed:39753558).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text>The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40999" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAB57605" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57605.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40999.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90217">
        <property type="entry name" value="H90217"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_014511594.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="F=1-214"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="F=1-211"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="F=1-214"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="F=1-214"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="F=1-214"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="F=1-214"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="F=1-214"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="F=1-214"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q9UX87"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q9UX87"/>
    <dbReference type="FunCoup" id="Q9UX87">
        <property type="interactions" value="271"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0698"/>
    <dbReference type="PaxDb" id="273057-SSO0698"/>
    <dbReference type="EnsemblBacteria" id="AAK40999">
        <property type="protein sequence ID" value="AAK40999"/>
        <property type="gene ID" value="SSO0698"/>
    </dbReference>
    <dbReference type="GeneID" id="1454966"/>
    <dbReference type="KEGG" id="sso:SSO0698"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.698"/>
    <dbReference type="eggNOG" id="arCOG04087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065898_0_1_2"/>
    <dbReference type="InParanoid" id="Q9UX87"/>
    <dbReference type="PhylomeDB" id="Q9UX87"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000002">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000004">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_A">
        <property type="entry name" value="Ribosomal_uS5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047866">
        <property type="entry name" value="Ribosomal_uS5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_uS5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003125">
        <property type="entry name" value="PRK04044.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF4">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000131661" description="Small ribosomal subunit protein uS5">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="S5 DRBM" evidence="1">
        <location>
            <begin position="54"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7 8">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7 8">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7 8">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7 8">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="CAB57605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FS6"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="10">
        <source>
            <dbReference type="PDB" id="9FS8"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="11">
        <source>
            <dbReference type="PDB" id="9FSF"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="12">
        <source>
            <dbReference type="PDB" id="9FY0"/>
        </source>
    </evidence>
    <sequence length="214" mass="23686" checksum="D8031272015D2FD3" modified="2002-12-06" version="2">MAEEVPSLNIEEWKPRTSIGSLVKEGKISSIKELFDRNLPITEPEIVDVLLPKLKYEVVDIKVVQKQTDAGEISRYKVLVIMGNMDGYVSIGTGKAKQLRVAIQKAIRDAKMNIIPVRRGCGSWQCTCGEPHSLPFKVVGKAGSVEVDLLPAPKGTGLVVGSVLKTLLTYAGIKDAWSTTKGETRTTENFVRAGYSALYNTYKFVTLQDWVRKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="145" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXA0</accession>
    <name>RS3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein uS3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">30S ribosomal protein S3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps3</name>
        <name evidence="1 4" type="synonym">rps3AB</name>
        <name type="ordered locus">SSO0712</name>
        <name type="ORF">C10_019</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="3">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds the lower part of the 30S subunit head.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S small ribosomal subunit, interacts with aS34.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90219">
        <property type="entry name" value="E90219"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991275.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="Z=1-229"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="Z=1-229"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="Z=1-229"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="Z=1-229"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="Z=1-229"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="Z=1-229"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="Z=1-229"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="Z=1-229"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q9UXA0"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q9UXA0"/>
    <dbReference type="FunCoup" id="Q9UXA0">
        <property type="interactions" value="249"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0712"/>
    <dbReference type="PaxDb" id="273057-SSO0712"/>
    <dbReference type="EnsemblBacteria" id="AAK41012">
        <property type="protein sequence ID" value="AAK41012"/>
        <property type="gene ID" value="SSO0712"/>
    </dbReference>
    <dbReference type="GeneID" id="1454979"/>
    <dbReference type="KEGG" id="sso:SSO0712"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.712"/>
    <dbReference type="eggNOG" id="arCOG04097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058591_1_1_2"/>
    <dbReference type="InParanoid" id="Q9UXA0"/>
    <dbReference type="PhylomeDB" id="Q9UXA0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02411">
        <property type="entry name" value="KH-II_30S_S3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000001">
        <property type="entry name" value="30S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_A">
        <property type="entry name" value="Ribosomal_uS3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027488">
        <property type="entry name" value="Ribosomal_uS3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_uS3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003219">
        <property type="entry name" value="PRK04191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000130262" description="Small ribosomal subunit protein uS3">
        <location>
            <begin position="1"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="1">
        <location>
            <begin position="18"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="CAB57592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="229" mass="25825" checksum="1EFD23A7E046DE1B" modified="2000-05-01" version="1">MPNIKRYFLEKSIVKVKIDEYLAKQYYNAEYAGVEVLKTPIGTRVIIYAGRPSMIIGRGGRNIKQLAQIFEKVFGLENPQITITNVENPELNARVMAFRLAIALEKGYHFRRAAFISMRRIMNAGALGAEIIISGKLTTERARYEKLKEGIVYKSGQQLEKMIDRAIAIAMLKPGIFGVEVVITKPLKIEDKINLKESPSVPQEVSVTNVTFIEESSQKSEEKSEGEKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-05" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXE7</accession>
    <name>G1PDH_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycerol-1-phosphate dehydrogenase [NAD(P)+]</fullName>
            <shortName evidence="1">G1P dehydrogenase</shortName>
            <shortName evidence="1">G1PDH</shortName>
            <ecNumber evidence="1">1.1.1.261</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Enantiomeric glycerophosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">sn-glycerol-1-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">egsA</name>
        <name type="synonym">gldA</name>
        <name type="ordered locus">SSO0760</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>sn-glycerol 1-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>sn-glycerol 1-phosphate + NADP(+) = dihydroxyacetone phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21416"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycerol-1-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.261" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90224">
        <property type="entry name" value="H90224"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991342.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXE7"/>
    <dbReference type="SMR" id="Q9UXE7"/>
    <dbReference type="FunCoup" id="Q9UXE7">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0760"/>
    <dbReference type="PaxDb" id="273057-SSO0760"/>
    <dbReference type="EnsemblBacteria" id="AAK41055">
        <property type="protein sequence ID" value="AAK41055"/>
        <property type="gene ID" value="SSO0760"/>
    </dbReference>
    <dbReference type="GeneID" id="1455022"/>
    <dbReference type="KEGG" id="sso:SSO0760"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.757"/>
    <dbReference type="eggNOG" id="arCOG00982">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038362_0_0_2"/>
    <dbReference type="InParanoid" id="Q9UXE7"/>
    <dbReference type="PhylomeDB" id="Q9UXE7"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008888">
        <property type="term" value="F:glycerol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106357">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106358">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08173">
        <property type="entry name" value="Gro1PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00497_A">
        <property type="entry name" value="G1P_dehydrogenase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023002">
        <property type="entry name" value="G1P_dehydrogenase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032837">
        <property type="entry name" value="G1PDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016205">
        <property type="entry name" value="Glycerol_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002022">
        <property type="entry name" value="PRK00843.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616">
        <property type="entry name" value="GLYCEROL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616:SF5">
        <property type="entry name" value="GLYCEROL DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13685">
        <property type="entry name" value="Fe-ADH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000112">
        <property type="entry name" value="Glycerol_dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000157353" description="Glycerol-1-phosphate dehydrogenase [NAD(P)+]">
        <location>
            <begin position="1"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="97"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="119"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00497"/>
        </source>
    </evidence>
    <sequence length="351" mass="38484" checksum="C930E3DC1CA636EF" modified="2000-05-01" version="1">MNVKEHVISLPRRVFVGHDIVYDISIYFSQLGVTPPFLIVTGTKYTKKIADKVIENLPKDAKYEVVEIDSATLDDVYMVEEVIKRISPSLLLGIGGGKVIDVTKYAAFRNSLEFVSIPTSPSHDGITSPFASIKGLQKPVSVKAKEPLAIIADIEILSLSPRRLINAGIGDTIGKIIAVRDWKLAAKLRGEYYGDYTASLALMSAKHAFQCTKIINKDIKYGVRMLMEALISSGVAMGMAGSTRPASGSEHLFAHAVELIHPEGILHGELVGLGTIIMAYLHGINWKIIRNRLKKIGFPVKAKDLGLSDEEVIKALTIAHTIRPERYTILGDRGLTWSSAEKIARVTKIID</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-20" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q9V2T7</accession>
    <name>THSG_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chaperonin subunit gamma</fullName>
            <ecNumber evidence="1">5.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Thermosome subunit gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">thsC</name>
        <name type="synonym">thsG</name>
        <name type="ordered locus">SSO3000</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1999" name="Curr. Biol." volume="9" first="1053" last="1056">
            <title>Recurrent paralogy in the evolution of archaeal chaperonins.</title>
            <authorList>
                <person name="Archibald J.M."/>
                <person name="Logsdon J.M. Jr."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="10508614"/>
            <dbReference type="DOI" id="10.1016/S0960-9822(99)80457-6"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2016" name="Structure" volume="24" first="364" last="374">
            <title>Structural and Functional Insights into the Evolution and Stress Adaptation of Type II Chaperonins.</title>
            <authorList>
                <person name="Chaston J.J."/>
                <person name="Smits C."/>
                <person name="Aragao D."/>
                <person name="Wong A.S."/>
                <person name="Ahsan B."/>
                <person name="Sandin S."/>
                <person name="Molugu S.K."/>
                <person name="Molugu S.K."/>
                <person name="Bernal R.A."/>
                <person name="Stock D."/>
                <person name="Stewart A.G."/>
            </authorList>
            <dbReference type="PubMed" id="26853941"/>
            <dbReference type="DOI" id="10.1016/j.str.2015.12.016"/>
        </citation>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; binds unfolded polypeptides and stimulates protein folding in a nucleotide-hydrolysis-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + an unfolded polypeptide = ADP + phosphate + a folded polypeptide.</text>
            <dbReference type="EC" id="5.6.1.7"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text evidence="2">Not thermostable, it unfolds at 75 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="2">The 3 paralogous subunits (alpha, beta and gamma) form oligomeric ring complexes, composition is temperature dependent. Gamma is unstable at 75 degrees Celsius, at 80 degrees Celsius in vivo there are 94-95% alpha-beta complexes with 5-6% beta-only rings, and no detectable gamma-containing rings. In the beta subunit-only complex 2 rings of 9 subunits each stack back-to-back to form an octadecameric complex. In the mixed alpha-beta complex there are 2 stacked 8-membered rings with alternating alpha and beta subunits making a hexadecameric complex; in the stacked rings alpha subunits stack on the beta subunits and only the beta subunits bind ADP. Only forms complexes with the alpha and beta subunits in the presence of ATP; the gamma-containing complexes have 2 rings of 9 subunits making an octadecameric complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK43104" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.7" evidence="1"/>
    <dbReference type="EMBL" id="AF149921">
        <property type="protein sequence ID" value="AAF03362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43104.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99481">
        <property type="entry name" value="A99481"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992650.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9V2T7"/>
    <dbReference type="SMR" id="Q9V2T7"/>
    <dbReference type="DIP" id="DIP-61908N"/>
    <dbReference type="FunCoup" id="Q9V2T7">
        <property type="interactions" value="269"/>
    </dbReference>
    <dbReference type="IntAct" id="Q9V2T7">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3000"/>
    <dbReference type="PaxDb" id="273057-SSO3000"/>
    <dbReference type="EnsemblBacteria" id="AAK43104">
        <property type="protein sequence ID" value="AAK43104"/>
        <property type="gene ID" value="SSO3000"/>
    </dbReference>
    <dbReference type="GeneID" id="1453023"/>
    <dbReference type="KEGG" id="sso:SSO3000"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3093"/>
    <dbReference type="eggNOG" id="arCOG01257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_9_1_2"/>
    <dbReference type="InParanoid" id="Q9V2T7"/>
    <dbReference type="PhylomeDB" id="Q9V2T7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000128403" description="Chaperonin subunit gamma">
        <location>
            <begin position="1"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q6LX38"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="26853941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="10508614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="535" mass="58770" checksum="31AA897DBD19924F" modified="2000-05-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2026-01-28" version="148" xmlns="https://uniprot.org/uniprot">
    <accession>O33770</accession>
    <name>HIS8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Histidinol-phosphate aminotransferase</fullName>
            <ecNumber>2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hisC</name>
        <name type="ordered locus">SSO0592</name>
        <name type="ORF">C08_058</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="J. Bacteriol." volume="179" first="4429" last="4432">
            <title>Evolutionary analysis of the hisCGABdFDEHI gene cluster from the archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Sensen C.W."/>
                <person name="Doolittle W.F."/>
                <person name="Brown J.R."/>
            </authorList>
            <dbReference type="PubMed" id="9209067"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9"/>
    <dbReference type="EMBL" id="U82227">
        <property type="protein sequence ID" value="AAB63018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90206">
        <property type="entry name" value="A90206"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991110.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O33770"/>
    <dbReference type="SMR" id="O33770"/>
    <dbReference type="FunCoup" id="O33770">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0592"/>
    <dbReference type="PaxDb" id="273057-SSO0592"/>
    <dbReference type="DNASU" id="1454871"/>
    <dbReference type="EnsemblBacteria" id="AAK40904">
        <property type="protein sequence ID" value="AAK40904"/>
        <property type="gene ID" value="SSO0592"/>
    </dbReference>
    <dbReference type="GeneID" id="1454871"/>
    <dbReference type="GeneID" id="44129594"/>
    <dbReference type="KEGG" id="sso:SSO0592"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.600"/>
    <dbReference type="eggNOG" id="arCOG04273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_1_2"/>
    <dbReference type="InParanoid" id="O33770"/>
    <dbReference type="PhylomeDB" id="O33770"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00012"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885:SF2">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000153505" description="Histidinol-phosphate aminotransferase">
        <location>
            <begin position="1"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="376" mass="43194" checksum="44CAB23CD2866405" modified="1998-01-01" version="1">MRFSLKLIYLFYVAYLGFYIAPTKLVRNKIKSWLLNASEYDFTDIKEGIRLHLNESPFEPPQFIIDAVKMYLSKGNRYQHPDLLEKYRELAAEYSKVEPENIYPSVGADGSIRAIFYNLVEPGDTILTNYPSYSMYSVYSSVRGTKVIKVNLKEDNEWWKENTDDLLAQAEKVELVIIDDPNNPTGSPMLNGKKELIGQLAENTKGFVVIDEAYYEFGGYTVSPYIYDYPNVLVVRTLSKAFSLASYRLGYTIANEEIVKALMKSSTPFDIPLPSLIAGITALENPSYIKDVVNIVNRNREILYQGLKNLNLKVYKSITNFLLIKDNRNLQEMLMRHGIAIRKLYDNFYRITIGTEDQCKMVIDKLGEELENSNSK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>P58110</accession>
    <name>TF2B2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription initiation factor IIB 2</fullName>
            <shortName evidence="1">TFIIB 2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tfbB</name>
        <name type="synonym">tfb-2</name>
        <name type="ordered locus">SSO0946</name>
        <name type="ORF">C33_019</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="J. Virol." volume="82" first="4874" last="4883">
            <title>Transcriptome analysis of infection of the archaeon Sulfolobus solfataricus with Sulfolobus turreted icosahedral virus.</title>
            <authorList>
                <person name="Ortmann A.C."/>
                <person name="Brumfield S.K."/>
                <person name="Walther J."/>
                <person name="McInnerney K."/>
                <person name="Brouns S.J."/>
                <person name="van de Werken H.J."/>
                <person name="Bothner B."/>
                <person name="Douglas T."/>
                <person name="van de Oost J."/>
                <person name="Young M.J."/>
            </authorList>
            <dbReference type="PubMed" id="18337566"/>
            <dbReference type="DOI" id="10.1128/jvi.02583-07"/>
        </citation>
        <scope>INDUCTION BY VIRUS (MICROBIAL INFECTION)</scope>
        <source>
            <strain>2-2-12</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="induction">
        <text evidence="3">(Microbial infection) At least 4-fold induced following infection by Sulfolobus turreted icosahedral virus 1 (STIV-1) in strain 2-2-12.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">Strain 2-2-12 is a substrain of P2 that is highly susceptible to infection by Sulfolobus turreted icosahedral virus 1 (STIV-1).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90245">
        <property type="entry name" value="F90245"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992381.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58110"/>
    <dbReference type="SMR" id="P58110"/>
    <dbReference type="FunCoup" id="P58110">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0946"/>
    <dbReference type="PaxDb" id="273057-SSO0946"/>
    <dbReference type="EnsemblBacteria" id="AAK41221">
        <property type="protein sequence ID" value="AAK41221"/>
        <property type="gene ID" value="SSO0946"/>
    </dbReference>
    <dbReference type="GeneID" id="1455188"/>
    <dbReference type="KEGG" id="sso:SSO0946"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.941"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_0_1_2"/>
    <dbReference type="InParanoid" id="P58110"/>
    <dbReference type="PhylomeDB" id="P58110"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000119336" description="Transcription initiation factor IIB 2">
        <location>
            <begin position="1"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="repeat" description="1">
        <location>
            <begin position="107"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="2">
        <location>
            <begin position="204"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TFIIB-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="3"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="18337566"/>
        </source>
    </evidence>
    <sequence length="293" mass="33284" checksum="F9F4B5F0D64085F7" modified="2001-06-01" version="1">MKCPYCKTDNAITYDVEKGMYVCTNCASVIEDSAVDPGPDWRAYNAKDRNEKERVGSPSTPKVHDWGFHTIIGYGRAKDRLKTLKMQRMQNKIRVSPKDKKLVTLLSILNDESSKLELPEHVKETASLIIRKMVETGLTKRIDQYTLIVAALYYSCQVNNIPRHLQEFKVRYSISSSEFWSALKRVQYVANSIPGFRPKIKPAEYIPKILYKLNLPPIIGTKASELVDLMHKQGLTSGKGYLSLSAASVYLISALMDIKKTQKEVADSLDITEVTIRNRYKDIVDNFDIVVTL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>P95967</accession>
    <name>PGP1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Phosphoglycolate phosphatase 1</fullName>
            <shortName>PGP 1</shortName>
            <shortName>PGPase 1</shortName>
            <ecNumber>3.1.3.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0094</name>
        <name type="ORF">C04028</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dephosphorylation of 2-phosphoglycolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>2-phosphoglycolate + H2O = glycolate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="EC" id="3.1.3.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal SPP-like hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.18"/>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="PIR" id="S75394">
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="SMR" id="P95967"/>
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    <dbReference type="STRING" id="273057.SSO0094"/>
    <dbReference type="PaxDb" id="273057-SSO0094"/>
    <dbReference type="EnsemblBacteria" id="AAK40451">
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        <property type="gene ID" value="SSO0094"/>
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    <dbReference type="GeneID" id="1453279"/>
    <dbReference type="KEGG" id="sso:SSO0094"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.91"/>
    <dbReference type="eggNOG" id="arCOG01213">
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    <dbReference type="HOGENOM" id="CLU_044146_2_0_2"/>
    <dbReference type="InParanoid" id="P95967"/>
    <dbReference type="PhylomeDB" id="P95967"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:magnesium ion binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07514">
        <property type="entry name" value="HAD_Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.10">
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01419">
        <property type="entry name" value="GPH_hydrolase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006382">
        <property type="entry name" value="PGPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01487">
        <property type="entry name" value="Pglycolate_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01482">
        <property type="entry name" value="SPP-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000:SF8">
        <property type="entry name" value="HAD SUPERFAMILY HYDROLASE-LIKE, TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01144">
        <property type="entry name" value="C2.B.4:_PGP_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00446">
        <property type="entry name" value="phosphoglycolate_phosphatase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000146728" description="Phosphoglycolate phosphatase 1">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="228" mass="25843" checksum="5FE13667B3A11199" modified="1997-05-01" version="1">MIKLVLVDLDGTLTEDRESTRIDLDAIYAIRLLQKSGIKVSLVSGNSYPILRGLYTYLYLDGGFVAENGCIVFYKEKYRMCRQMEQSLVDEFKSLFKLRDTWQNEYRECDFGFVPAKITDEMINWAKERNLYIKSSGYAVHIAYNPAGKRIGVEKLLQLLGLKKEDVAAIGDSSTDIELFQQVGFKVAVGNADDELKDIADYITSNKSGKGVREFVDKLLKGEFDGIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>P95980</accession>
    <name>DNAG_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA primase DnaG</fullName>
            <ecNumber evidence="1 4">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaG</name>
        <name type="ordered locus">SSO0079</name>
        <name type="ORF">C04_042</name>
        <name type="ORF">C05_030</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="EMBO Rep." volume="4" first="889" last="893">
            <title>An exosome-like complex in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Evguenieva-Hackenberg E."/>
                <person name="Walter P."/>
                <person name="Hochleitner E."/>
                <person name="Lottspeich F."/>
                <person name="Klug G."/>
            </authorList>
            <dbReference type="PubMed" id="12947419"/>
            <dbReference type="DOI" id="10.1038/sj.embor.embor929"/>
        </citation>
        <scope>INTERACTION WITH EXOSOME</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2006" name="Mol. Microbiol." volume="62" first="1076" last="1089">
            <title>Characterization of native and reconstituted exosome complexes from the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Walter P."/>
                <person name="Klein F."/>
                <person name="Lorentzen E."/>
                <person name="Ilchmann A."/>
                <person name="Klug G."/>
                <person name="Evguenieva-Hackenberg E."/>
            </authorList>
            <dbReference type="PubMed" id="17078816"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.2006.05393.x"/>
        </citation>
        <scope>INTERACTION WITH EXOSOME</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2010" name="J. Mol. Biol." volume="397" first="664" last="676">
            <title>Characterization of a functional DnaG-type primase in archaea: implications for a dual-primase system.</title>
            <authorList>
                <person name="Zuo Z."/>
                <person name="Rodgers C.J."/>
                <person name="Mikheikin A.L."/>
                <person name="Trakselis M.A."/>
            </authorList>
            <dbReference type="PubMed" id="20122937"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2010.01.057"/>
        </citation>
        <scope>FUNCTION AS A PRIMASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF GLU-175</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2012" name="Biochimie" volume="94" first="1578" last="1587">
            <title>Heterogeneous complexes of the RNA exosome in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Witharana C."/>
                <person name="Roppelt V."/>
                <person name="Lochnit G."/>
                <person name="Klug G."/>
                <person name="Evguenieva-Hackenberg E."/>
            </authorList>
            <dbReference type="PubMed" id="22503705"/>
            <dbReference type="DOI" id="10.1016/j.biochi.2012.03.026"/>
        </citation>
        <scope>INTERACTION WITH EXOSOME</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2013" name="J. Mol. Biol." volume="425" first="1259" last="1273">
            <title>Novel interaction of the bacterial-Like DnaG primase with the MCM helicase in archaea.</title>
            <authorList>
                <person name="Bauer R.J."/>
                <person name="Graham B.W."/>
                <person name="Trakselis M.A."/>
            </authorList>
            <dbReference type="PubMed" id="23357171"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2013.01.025"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH MCM AND DNA</scope>
        <scope>MUTAGENESIS OF ASP-179; ASP-220 AND ASP-222</scope>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2013" name="RNA Biol." volume="10" first="415" last="424">
            <title>The archaeal DnaG protein needs Csl4 for binding to the exosome and enhances its interaction with adenine-rich RNAs.</title>
            <authorList>
                <person name="Hou L."/>
                <person name="Klug G."/>
                <person name="Evguenieva-Hackenberg E."/>
            </authorList>
            <dbReference type="PubMed" id="23324612"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH CSL4</scope>
    </reference>
    <comment type="function">
        <text evidence="1 4 6 7">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Can use NTPs but not dNTPs. Binds DNA. Also part of the exosome, which is a complex involved in RNA degradation. Acts as a poly(A)-binding protein that enhances the interaction between heteromeric, adenine-rich transcripts and the exosome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.</text>
            <dbReference type="EC" id="2.7.7.101"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds two Mg(2+) per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="4">85 uM for NTPs</KM>
            <Vmax evidence="4">0.72 pmol/min/ug enzyme</Vmax>
            <text evidence="4">kcat is 0.033 min(-1).</text>
        </kinetics>
        <temperatureDependence>
            <text evidence="4">Optimum temperature is 70 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3 4 5 6 7">Forms a ternary complex with MCM helicase and DNA. Component of the archaeal exosome complex. Interacts with Csl4 but not with Rrp4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal DnaG primase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="1 4"/>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75408">
        <property type="entry name" value="S75408"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95980"/>
    <dbReference type="SMR" id="P95980"/>
    <dbReference type="FunCoup" id="P95980">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="IntAct" id="P95980">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="MINT" id="P95980"/>
    <dbReference type="STRING" id="273057.SSO0079"/>
    <dbReference type="PaxDb" id="273057-SSO0079"/>
    <dbReference type="EnsemblBacteria" id="AAK40440">
        <property type="protein sequence ID" value="AAK40440"/>
        <property type="gene ID" value="SSO0079"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0079"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.79"/>
    <dbReference type="eggNOG" id="arCOG04281">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034626_0_0_2"/>
    <dbReference type="InParanoid" id="P95980"/>
    <dbReference type="PhylomeDB" id="P95980"/>
    <dbReference type="BRENDA" id="2.7.7.101">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1360.10:FF:000010">
        <property type="entry name" value="DNA primase DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00007">
        <property type="entry name" value="DNA_primase_DnaG_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050219">
        <property type="entry name" value="DnaG_primase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020607">
        <property type="entry name" value="Primase_DnaG_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003108">
        <property type="entry name" value="PRK04031.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30313">
        <property type="entry name" value="DNA PRIMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30313:SF2">
        <property type="entry name" value="DNA PRIMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110455">
        <property type="entry name" value="Toprim domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0271">Exosome</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0639">Primosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000144132" description="DNA primase DnaG">
        <location>
            <begin position="1"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="1">
        <location>
            <begin position="169"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Strong decrease of primase activity." evidence="4">
        <original>E</original>
        <variation>Q</variation>
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Decreases binding affinity to DNA. Does not affect primase activity." evidence="7">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Decreases binding affinity to DNA and primase activity." evidence="7">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Decreases binding affinity to DNA and primase activity." evidence="7">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12947419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="17078816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="20122937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="22503705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="23324612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="23357171"/>
        </source>
    </evidence>
    <sequence length="406" mass="45410" checksum="26E5B615560C6EF1" modified="1997-07-01" version="2">MSFQMKYDIRLRFEVEGIVEKTDVIGAIFGQTENLFGDEFDLRELQDKGRLGRIIVEVKTKGGKSEGEIIIPSNLDRIETALIAAMVESVDKVGPYNSKFELIEIEDIRAEKLKKIIERAKGILSSWSKEKSLDIKEVINEISSAVKVGEITEYGPERLPAGPDVDKDPNLIIVEGRADVINLLRYGYKNVIAVEGATSRIPETLINLSKMKKTVIAFLDGDHGGDLILKELLSNNVKIDFVARAPIGREVEELTGKEIAKALSNMMPLTQYLKKVQEAEQAIAKNVIAKEEKPIQSETTQQVVQITLPQNILEEIKKLPGTLEGVLYDNNWNLIEKVQVRDIIPKLEAYEDNKVAYIIFDGVITQRLLDLASQKNIKMIIGARIGGINKRPQNVDILTFTDIISS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>P96040</accession>
    <name>RL12_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein P1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">50S ribosomal protein L12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl12</name>
        <name type="synonym">rpl12Ab</name>
        <name type="ordered locus">SSO0342</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="Biochim. Biophys. Acta" volume="1340" first="170" last="177">
            <title>Nucleotide sequence of a gene cluster encoding NusG and the L11-L1-L10-L12 ribosomal proteins from the thermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Geiger M."/>
                <person name="Groebner P."/>
                <person name="Piendl W."/>
            </authorList>
            <dbReference type="PubMed" id="9252104"/>
            <dbReference type="DOI" id="10.1016/S0167-4838(97)00073-3"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Homodimer, it forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric uL10/P0(P1)2(P1)2(P1)2 complex, where uL10/P0 forms an elongated spine to which the P1 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="U85262">
        <property type="protein sequence ID" value="AAB99527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90177">
        <property type="entry name" value="A90177"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990630.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P96040"/>
    <dbReference type="SMR" id="P96040"/>
    <dbReference type="FunCoup" id="P96040">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0342"/>
    <dbReference type="PaxDb" id="273057-SSO0342"/>
    <dbReference type="EnsemblBacteria" id="AAK40672">
        <property type="protein sequence ID" value="AAK40672"/>
        <property type="gene ID" value="SSO0342"/>
    </dbReference>
    <dbReference type="GeneID" id="1455482"/>
    <dbReference type="KEGG" id="sso:SSO0342"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.333"/>
    <dbReference type="eggNOG" id="arCOG04287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114656_2_0_2"/>
    <dbReference type="InParanoid" id="P96040"/>
    <dbReference type="PhylomeDB" id="P96040"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05832">
        <property type="entry name" value="Ribosomal_L12p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022295">
        <property type="entry name" value="Ribosomal_P1_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03685">
        <property type="entry name" value="ribo_P1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000157637" description="Large ribosomal subunit protein P1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="106" mass="11291" checksum="38879BB1C0861653" modified="1997-05-01" version="1">MEYIYASLLLHSAKKEISEDALKNVLTAAGISVDEVRLKAVVAALKEVNIDEVLKNAAAMPVAVAAQPQATQAQPAAEEKKEEKKEEEKKGPSEEEIASGLASLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX46</accession>
    <name>Q7LX46_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-haloalkanoic acid dehalogenase</fullName>
            <ecNumber evidence="2">3.8.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.8.1.2" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74066">
        <property type="entry name" value="S74066"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992154.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX46"/>
    <dbReference type="FunCoup" id="Q7LX46">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2159"/>
    <dbReference type="PaxDb" id="273057-SSO2159"/>
    <dbReference type="EnsemblBacteria" id="AAK42335">
        <property type="protein sequence ID" value="AAK42335"/>
        <property type="gene ID" value="SSO2159"/>
    </dbReference>
    <dbReference type="GeneID" id="1453662"/>
    <dbReference type="KEGG" id="sso:SSO2159"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2252"/>
    <dbReference type="eggNOG" id="arCOG02291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_8_0_2"/>
    <dbReference type="InParanoid" id="Q7LX46"/>
    <dbReference type="PhylomeDB" id="Q7LX46"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018784">
        <property type="term" value="F:(S)-2-haloacid dehalogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051828">
        <property type="entry name" value="HAD-like_hydrolase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191:SF2">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="222" mass="25448" checksum="143F8DE5B2647866" modified="2011-05-31" version="1">MKTIFVDLGETLVHFKPRYHENIAYALKEIGYNVDERRVFKAVAKILGKHHYPSQEYGGLSAFDFRELFYELNIYPDEQLITRLNSKNLLSGEYELYDDSITFLEEAKGLGFKLVLVSNATRSIYKIVEDLGIKKYFDGIVASCDLNIMKPHPKIFSYAMEIAKSDGIHIGDIYEIDVIGAKRAGLEAILLDRLGFYPEIRENKVSNLLEALELVKEKLRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-06-18" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX61</accession>
    <name>Q7LX61_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acylaminoacyl-peptidase, putative (ApeH-2)</fullName>
            <ecNumber evidence="4">3.4.19.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">apeH-2</name>
        <name evidence="4" type="ordered locus">SSO2141</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.19.1" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74053">
        <property type="entry name" value="S74053"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX61"/>
    <dbReference type="FunCoup" id="Q7LX61">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2141"/>
    <dbReference type="ESTHER" id="sulso-APEH2">
        <property type="family name" value="ACPH_Peptidase_S9"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2141"/>
    <dbReference type="EnsemblBacteria" id="AAK42319">
        <property type="protein sequence ID" value="AAK42319"/>
        <property type="gene ID" value="SSO2141"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2141"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2234"/>
    <dbReference type="eggNOG" id="arCOG01646">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_482043_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX61"/>
    <dbReference type="PhylomeDB" id="Q7LX61"/>
    <dbReference type="BRENDA" id="3.4.19.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.150">
        <property type="entry name" value="Peptidase/esterase 'gauge' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054035">
        <property type="entry name" value="APH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF27">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE FAMILY MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22173">
        <property type="entry name" value="APH-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50993">
        <property type="entry name" value="Peptidase/esterase 'gauge' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylamino-acid-releasing enzyme-like N-terminal" evidence="3">
        <location>
            <begin position="37"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="2">
        <location>
            <begin position="371"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="569" mass="64283" checksum="7698B2A1ABB96E37" modified="2011-05-31" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-02-05" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX92</accession>
    <name>Q7LX92_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Third ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74015">
        <property type="entry name" value="S74015"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX92"/>
    <dbReference type="STRING" id="273057.SSO2103"/>
    <dbReference type="PaxDb" id="273057-SSO2103"/>
    <dbReference type="EnsemblBacteria" id="AAK42282">
        <property type="protein sequence ID" value="AAK42282"/>
        <property type="gene ID" value="SSO2103"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2103"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2186"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171556_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX92"/>
    <dbReference type="PhylomeDB" id="Q7LX92"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="112" mass="13125" checksum="40F7148F63FCD95B" modified="2011-05-31" version="1">MTKDEIVKILIEQVVAMGFRIKLIALDAGFYTVEVIKFISQFNYIIGVPVSDVKIYEEFDGEYVTNSKRRSKGEQVKFRLIVYREKIKRKKKEVVYFARGTNLDLPKNKVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXN3</accession>
    <name>Q7LXN3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Molybdopterin-guanine dinucleotide biosynthesis protein A (MobA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mobA</name>
        <name evidence="9" type="ordered locus">SSO0677</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90215">
        <property type="entry name" value="D90215"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991224.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXN3"/>
    <dbReference type="SMR" id="Q7LXN3"/>
    <dbReference type="STRING" id="273057.SSO0677"/>
    <dbReference type="PaxDb" id="273057-SSO0677"/>
    <dbReference type="EnsemblBacteria" id="AAK40979">
        <property type="protein sequence ID" value="AAK40979"/>
        <property type="gene ID" value="SSO0677"/>
    </dbReference>
    <dbReference type="GeneID" id="1454946"/>
    <dbReference type="KEGG" id="sso:SSO0677"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.677"/>
    <dbReference type="eggNOG" id="arCOG01872">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055597_2_1_2"/>
    <dbReference type="InParanoid" id="Q7LXN3"/>
    <dbReference type="PhylomeDB" id="Q7LXN3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02503">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013482">
        <property type="entry name" value="Molybde_CF_guanTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136:SF81">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MobA-like NTP transferase" evidence="8">
        <location>
            <begin position="9"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK40979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="194" mass="22319" checksum="AA87E82DE74CD6AB" modified="2011-05-31" version="1">MSYNNSYDVIILAGGLSKRFGADKCCFEVNSKTMLDRLLEQFDNPIVVSRSPRRIKKGILVIEKGEYEGPVKGVREGLKYVRRDRVFITGCDFPFLIKNLVDYVCSKPYDIVIPFDHRPQPLLACYSTELLNKNIDKINGLVELIELSSSVYFIGTEELLKIDPFLYSLININSIIDLIYRPIRIKTKSNFILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXN4</accession>
    <name>Q7LXN4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdopterin biosynthesis protein (MoeA-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">moeA-1</name>
        <name evidence="2" type="ordered locus">SSO0676</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90215">
        <property type="entry name" value="C90215"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991222.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXN4"/>
    <dbReference type="FunCoup" id="Q7LXN4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0676"/>
    <dbReference type="PaxDb" id="273057-SSO0676"/>
    <dbReference type="EnsemblBacteria" id="AAK40978">
        <property type="protein sequence ID" value="AAK40978"/>
        <property type="gene ID" value="SSO0676"/>
    </dbReference>
    <dbReference type="GeneID" id="1454945"/>
    <dbReference type="KEGG" id="sso:SSO0676"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.676"/>
    <dbReference type="eggNOG" id="arCOG00216">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_7_2_2"/>
    <dbReference type="InParanoid" id="Q7LXN4"/>
    <dbReference type="PhylomeDB" id="Q7LXN4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.190.11:FF:000016">
        <property type="entry name" value="Molybdenum cofactor synthesis domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.10">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.190.11">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF19">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN MJ0666-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="MoeA N-terminal region -like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="1">
        <location>
            <begin position="177"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="389" mass="43235" checksum="4E5CC6248B15BA72" modified="2011-05-31" version="1">MALIPVEEARKIISNTNFEVKRREKYVEIFQALGKIAVEDILAVKSMPERSLSAMDGYAIKYDDYLKYGKLRVVGKLYPSTSNIPELKEGETYYVTTGSPLPLYADSVVPVENSRLEGEYVIFKGEVKKGKNIREEGEDINIGQIIIQKGTEITPYYLGILIQQKIFHVKVSDLKFTVFANGDEITSFENPQKNKIIDSISPILIKILEKFGSVKYMGVAKDDLNDVTEKIEKSLEISDVIISVGGSSVGEKDYVKKAVNKLGEVLFDGVSVNTIKRGSVGRIQGKPILILPGQVVSAITAFHEFGLSVLSNILGTNLKKFIKSTLVEDIYVNHSMDSVYLFNVKGNSAFPLRWGVGLYSELAKANGFAILKRGLTYKKGEEVELQQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXQ0</accession>
    <name>Q7LXQ0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99213">
        <property type="entry name" value="A99213"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991193.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXQ0"/>
    <dbReference type="PaxDb" id="273057-SSO0655"/>
    <dbReference type="EnsemblBacteria" id="AAK40960">
        <property type="protein sequence ID" value="AAK40960"/>
        <property type="gene ID" value="SSO0655"/>
    </dbReference>
    <dbReference type="GeneID" id="1454927"/>
    <dbReference type="KEGG" id="sso:SSO0655"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.657"/>
    <dbReference type="eggNOG" id="arCOG07286">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1444694_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXQ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="183" mass="21011" checksum="88DFEAFF04CB7711" modified="2011-05-31" version="1">MTRRDIIISIISALPPIIGTTITSFIMSRRYGDLKEVIKYVIIGLPVTLAITYYLILNIFPKYVDYLIAIPALLPLPISFLLTRNNISINYSEDYIEIKFKIPINMTSHDPHELFNYVFNKALGKIRNPYYHYKLSAAANCSGLKVSYQNEDKLVIRRKCGDIDIRIIISSKKIADMVIDISY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXS9</accession>
    <name>Q7LXS9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIP18 family-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0601</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90207">
        <property type="entry name" value="B90207"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991123.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXS9"/>
    <dbReference type="FunCoup" id="Q7LXS9">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0601"/>
    <dbReference type="PaxDb" id="273057-SSO0601"/>
    <dbReference type="EnsemblBacteria" id="AAK40913">
        <property type="protein sequence ID" value="AAK40913"/>
        <property type="gene ID" value="SSO0601"/>
    </dbReference>
    <dbReference type="GeneID" id="1454880"/>
    <dbReference type="KEGG" id="sso:SSO0601"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.609"/>
    <dbReference type="eggNOG" id="arCOG01845">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091588_2_2_2"/>
    <dbReference type="InParanoid" id="Q7LXS9"/>
    <dbReference type="PhylomeDB" id="Q7LXS9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052339">
        <property type="entry name" value="Fe-S_Maturation_MIP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831:SF1">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="1">
        <location>
            <begin position="14"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="132" mass="15113" checksum="F233AC202F9BAB6E" modified="2011-05-31" version="1">MSSQQQKVDKEEWKKKIMEALKDVYDPEIPVDIVNLGLIYDLKINDDGDVYLRLGLTAPGCPVVDDLIYTVEQVIKESVPAKSVEVDIDLDTQWTPLKMTAEGREKFKQLYGYDIVEMWVQTYGLPADEQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXW3</accession>
    <name>Q7LXW3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0532</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90199">
        <property type="entry name" value="E90199"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991042.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXW3"/>
    <dbReference type="STRING" id="273057.SSO0532"/>
    <dbReference type="PaxDb" id="273057-SSO0532"/>
    <dbReference type="EnsemblBacteria" id="AAK40852">
        <property type="protein sequence ID" value="AAK40852"/>
        <property type="gene ID" value="SSO0532"/>
    </dbReference>
    <dbReference type="GeneID" id="1454819"/>
    <dbReference type="KEGG" id="sso:SSO0532"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.531"/>
    <dbReference type="eggNOG" id="arCOG01285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119412_2_2_2"/>
    <dbReference type="InParanoid" id="Q7LXW3"/>
    <dbReference type="PhylomeDB" id="Q7LXW3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="ChsH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="ChsH2_Znr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF5">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_ChsH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="2">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="ChsH2 C-terminal OB-fold" evidence="1">
        <location>
            <begin position="49"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="14937" checksum="7A6E83EECD3DA8E7" modified="2011-05-31" version="1">MKIAPPQVWRNKRFMYKLTASRCERCGHISFPYSSYCTKCGSKNVKKIEISGKGVLESYTVSYQSRDGHEKGLPSVIGLVRLDEGVEIVAPIVDADLDKLKEGVKVEATLRRVYTDSYNGLIQYGIKFRVAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXW9</accession>
    <name>Q7LXW9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0522</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99198">
        <property type="entry name" value="A99198"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923023.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXW9"/>
    <dbReference type="STRING" id="273057.SSO0522"/>
    <dbReference type="PaxDb" id="273057-SSO0522"/>
    <dbReference type="EnsemblBacteria" id="AAK40840">
        <property type="protein sequence ID" value="AAK40840"/>
        <property type="gene ID" value="SSO0522"/>
    </dbReference>
    <dbReference type="GeneID" id="1454807"/>
    <dbReference type="KEGG" id="sso:SSO0522"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.517"/>
    <dbReference type="eggNOG" id="arCOG08511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079810_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXW9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="303" mass="32936" checksum="30A35C1E820DAD0E" modified="2011-05-31" version="1">MKRHLLLVAPLFLLLISLNALAVTANQLGASTILTTYNSDNWAGVAYADEWRSGVTNYWAVVQWASESIYVGNLSFAYAGYTNPNAVSIWVGLSPAKIGVGTDASTSEVTNTFAQAGYVIHVYSNGVVTAEYFVMVLNNGQILPNFVEAPVPAGVPAYLDVFLDNLFNYTELAQFYVFYKNGSYWSTSVYISTPFNTTGSAMSIVEAPQPTENGNNHFELPLMQGGMIHFTFGYVGPNKNDYSGPGNAPSGTTMYAVLFNLNEASYYNTAEAVIANGWANSLGGWDYLYQFIYPDLGITTTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LYL8</accession>
    <name>Q7LYL8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99224">
        <property type="entry name" value="A99224"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923059.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LYL8"/>
    <dbReference type="PaxDb" id="273057-SSO0753"/>
    <dbReference type="EnsemblBacteria" id="AAK41048">
        <property type="protein sequence ID" value="AAK41048"/>
        <property type="gene ID" value="SSO0753"/>
    </dbReference>
    <dbReference type="GeneID" id="71027413"/>
    <dbReference type="KEGG" id="sso:SSO0753"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.749"/>
    <dbReference type="eggNOG" id="arCOG07720">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850726_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LYL8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="138" mass="15015" checksum="88DAF8A2A0B527F6" modified="2011-05-31" version="1">MPSEYCTRIVVGTVFQPFTLDFKFNDIDIGAPLFSRQFCATYCSEFGLVGLIKSFSTRAINVPLGNNLQYPVEFVFLDVIKPPTDLTTAGIANYTKELNISKGFGVIIDALNKEKKAIAGISAVFPLAIAELAVLAID</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TZ6</accession>
    <name>COBS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenosylcobinamide-GDP ribazoletransferase</fullName>
            <ecNumber evidence="1">2.7.8.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cobalamin synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Cobalamin-5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cobS</name>
        <name type="ordered locus">SSO3234</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-ribazole + adenosylcob(III)inamide-GDP = adenosylcob(III)alamin + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16049"/>
            <dbReference type="ChEBI" id="CHEBI:10329"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-ribazole 5'-phosphate + adenosylcob(III)inamide-GDP = adenosylcob(III)alamin 5'-phosphate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23560"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57918"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="ChEBI" id="CHEBI:60493"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 7/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CobS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.26" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90509">
        <property type="entry name" value="A90509"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989675.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TZ6"/>
    <dbReference type="FunCoup" id="Q97TZ6">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3234"/>
    <dbReference type="PaxDb" id="273057-SSO3234"/>
    <dbReference type="DNASU" id="1453247"/>
    <dbReference type="EnsemblBacteria" id="AAK43328">
        <property type="protein sequence ID" value="AAK43328"/>
        <property type="gene ID" value="SSO3234"/>
    </dbReference>
    <dbReference type="GeneID" id="1453247"/>
    <dbReference type="GeneID" id="44128947"/>
    <dbReference type="KEGG" id="sso:SSO3234"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3336"/>
    <dbReference type="eggNOG" id="arCOG04338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057426_2_0_2"/>
    <dbReference type="InParanoid" id="Q97TZ6"/>
    <dbReference type="PhylomeDB" id="Q97TZ6"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00238"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051073">
        <property type="term" value="F:adenosylcobinamide-GDP ribazoletransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008818">
        <property type="term" value="F:cobalamin 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00719">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003805">
        <property type="entry name" value="CobS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00317">
        <property type="entry name" value="cobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-GDP RIBAZOLETRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148:SF1">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-GDP RIBAZOLETRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02654">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000146921" description="Adenosylcobinamide-GDP ribazoletransferase">
        <location>
            <begin position="1"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00719"/>
        </source>
    </evidence>
    <sequence length="253" mass="27831" checksum="D69B03290DE5C68A" modified="2001-10-01" version="1">MHLKEILAQFSFFTAIPVKSSASLEEIAESSYISPIIVGISLGLIESVAYLILYRILGELTGIVLLGIIELLRGFNHLDGLLDLGDALMIRGNREKKIKALKDVEVGSGGIGLLLVYLSIQIVALLKLDFSLYTIFYLISSNVLSMSLSLYILSTISPIPESNLGRIFHDKLKGKSTILLLELIPFISLYNVIVFIIFYMIMYKICGSLGGSSGDIAGASITLSFPLFLITDEITNLNYSLLSILCYLFSHLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U06</accession>
    <name>Q97U06_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO3219</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDPGP type 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90507">
        <property type="entry name" value="F90507"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989687.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U06"/>
    <dbReference type="STRING" id="273057.SSO3219"/>
    <dbReference type="PaxDb" id="273057-SSO3219"/>
    <dbReference type="EnsemblBacteria" id="AAK43317">
        <property type="protein sequence ID" value="AAK43317"/>
        <property type="gene ID" value="SSO3219"/>
    </dbReference>
    <dbReference type="GeneID" id="1453236"/>
    <dbReference type="KEGG" id="sso:SSO3219"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3324"/>
    <dbReference type="eggNOG" id="arCOG00664">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_1_2_2"/>
    <dbReference type="InParanoid" id="Q97U06"/>
    <dbReference type="PhylomeDB" id="Q97U06"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005771">
        <property type="entry name" value="GalU_uridylyltTrfase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197">
        <property type="entry name" value="UTP--GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197:SF1">
        <property type="entry name" value="UTP--GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="6">
        <location>
            <begin position="4"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="253" mass="28509" checksum="DD79F837F9AD7FB7" modified="2001-10-01" version="1">MLEKAVITSAGKGSRMKHITNVLPKALLPLFVTENGGKVTRPVIDLIMDSLNKIGVEKFCIVVGRNGMLLMQYLFDRTPTFVFQDMPKGFGDAVLRAEDFSTNEPFFVHADDGVLTKGYDSLKLLFNEISPDGVLLVRRVENPSRYGVVTVQDKGMYNGHKLYRVIDAEEKPLHPKSNLAIAAVYIFKPSIFSALRQIKVEEGKELELTYGIHNLLLDGKEVYALEMNEDEKWLNVGDPKSYLDALNYSFKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U69</accession>
    <name>Q97U69_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO12083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90499">
        <property type="entry name" value="F90499"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991894.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U69"/>
    <dbReference type="PaxDb" id="273057-SSO12083"/>
    <dbReference type="EnsemblBacteria" id="AAK43253">
        <property type="protein sequence ID" value="AAK43253"/>
        <property type="gene ID" value="SSO12083"/>
    </dbReference>
    <dbReference type="GeneID" id="1453172"/>
    <dbReference type="KEGG" id="sso:SSO12083"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3258"/>
    <dbReference type="eggNOG" id="arCOG06096">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2271157_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U69"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="87" mass="9089" checksum="135166D41F7A5EA2" modified="2001-10-01" version="1">MIGRILSGIGGIIALIASLVSITTREPLINHLVKILLITGDAVVGMGIIGLIGSLTTIYGVYKNNWKYMVSGGILGLFAPLLLPVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U78</accession>
    <name>Q97U78_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase (Acd-7)</fullName>
            <ecNumber evidence="9">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">acd-7</name>
        <name evidence="9" type="ordered locus">SSO3145</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="9"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90498">
        <property type="entry name" value="D90498"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990908.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U78"/>
    <dbReference type="STRING" id="273057.SSO3145"/>
    <dbReference type="PaxDb" id="273057-SSO3145"/>
    <dbReference type="EnsemblBacteria" id="AAK43243">
        <property type="protein sequence ID" value="AAK43243"/>
        <property type="gene ID" value="SSO3145"/>
    </dbReference>
    <dbReference type="GeneID" id="1453162"/>
    <dbReference type="KEGG" id="sso:SSO3145"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3249"/>
    <dbReference type="eggNOG" id="arCOG04350">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_2"/>
    <dbReference type="InParanoid" id="Q97U78"/>
    <dbReference type="PhylomeDB" id="Q97U78"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00567">
        <property type="entry name" value="ACAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF37">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="8">
        <location>
            <begin position="8"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="7">
        <location>
            <begin position="122"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="6">
        <location>
            <begin position="231"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK43243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="389" mass="44073" checksum="A6950774B5362B07" modified="2001-10-01" version="1">MLLQPKDEEEKLILSTVDQLMDKYNERYWLDKDQKREFPIDFLNDFMKLGLGSILIPVEYGGVGKGVRLASLILYNINLRGGNSYFVHGHYYNTALLSKHSGKRIIEKYFKDLANGAKVLSLALTEPQVGSDTTKIKTVAEKVGNSFFIKGHKIFISRLKYTDFMILVARTTPYEKVEKKTDGITLFLVDLREGRDGIEMREIKTMSNTNAYEVFIDGLKVPEDNVIGEVGKGFYYLLDLLNAERFMIAAEMIGNAEWFINKAVEYAKNRVVFDKQIGSFQGVQFPIAEVYAKLVSLSSYFNEGLRVIEKGKDTKTVGSYANISKYLATEIAWEAGNVAMDVYGGYGYAVETGIERKLRETRLYKVAPISQNLILAYIAHHILGLPKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UC3</accession>
    <name>Q97UC3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90492">
        <property type="entry name" value="G90492"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924131.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UC3"/>
    <dbReference type="STRING" id="273057.SSO3092"/>
    <dbReference type="PaxDb" id="273057-SSO3092"/>
    <dbReference type="EnsemblBacteria" id="AAK43198">
        <property type="protein sequence ID" value="AAK43198"/>
        <property type="gene ID" value="SSO3092"/>
    </dbReference>
    <dbReference type="GeneID" id="1453117"/>
    <dbReference type="KEGG" id="sso:SSO3092"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3200"/>
    <dbReference type="eggNOG" id="arCOG08497">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_830598_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UC3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="294"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="316"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="334" mass="37974" checksum="97622ED88EBF26BB" modified="2001-10-01" version="1">MRFSYIFMQEFKRSIVFVFLILIALSLSLIAAQILSESISVIGIVEGSSYGIKVSGYVFDFQGKGVSSEIKLSYYNFTESVKTNNGSFYLYLPVAYIQNNTRINVIINSFQKNVNLTLTIPNSSTTFAFSSLFLKYYSSQNLSIISVKGKTFIAVDPKQENSNYTIFEINSDNVNLPLDVNFTYNNFSNTLKIPLIILLLFFFSFIDLMDYYLLSSFPRGELRQIMRLVGTIKVYVGKLIAEIPLTIMLSSLPFYLFEFILIGNDIALILPYMIASISFSLGVYGLAQFGSKNMIYYSVILGFYIVNLVIYDRYLYSLLATLLLIVGYIKLRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UE3</accession>
    <name>Q97UE3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90490">
        <property type="entry name" value="C90490"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989090.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UE3"/>
    <dbReference type="SMR" id="Q97UE3"/>
    <dbReference type="STRING" id="273057.SSO3078"/>
    <dbReference type="PaxDb" id="273057-SSO3078"/>
    <dbReference type="EnsemblBacteria" id="AAK43178">
        <property type="protein sequence ID" value="AAK43178"/>
        <property type="gene ID" value="SSO3078"/>
    </dbReference>
    <dbReference type="GeneID" id="7798440"/>
    <dbReference type="KEGG" id="sso:SSO3078"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3180"/>
    <dbReference type="eggNOG" id="arCOG02730">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153004_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UE3"/>
    <dbReference type="PhylomeDB" id="Q97UE3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18684">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39664">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39664:SF2">
        <property type="entry name" value="NUCLEIC ACID-BINDING PROTEIN, CONTAINING PIN DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="2"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="134" mass="15459" checksum="E0CAC16D74E039A5" modified="2001-10-01" version="1">MEKAIVDTNVIIYDYVEDSEYHKRAEELLDSLDRWVIPVIVVHEIMWFLKGMKLEDKVDDVLAYIRSEKAEVVCDCTDNVTDAIGVLNKEKLPLVSYKDMVILSHAIREKLPLATFDRKLSKIAKKYSVSLLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UE6</accession>
    <name>Q97UE6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Partial transposase in ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90489">
        <property type="entry name" value="H90489"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UE6"/>
    <dbReference type="STRING" id="273057.SSO3076"/>
    <dbReference type="PaxDb" id="273057-SSO3076"/>
    <dbReference type="EnsemblBacteria" id="AAK43175">
        <property type="protein sequence ID" value="AAK43175"/>
        <property type="gene ID" value="SSO3076"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3076"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3178"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177472_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UE6"/>
    <dbReference type="PhylomeDB" id="Q97UE6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="104" mass="11431" checksum="E9B915F9487D4705" modified="2001-10-01" version="1">MLGLLIPSYSRSSRILGGRRATRMMLAEIVVSMGSGFTTSEARELTSQWDFVTRSIARVKNRLRRDLILLGYKDSLSKKNLEEVLRGGDSIVLSEVSLGGVGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UG8</accession>
    <name>Q97UG8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99487">
        <property type="entry name" value="A99487"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UG8"/>
    <dbReference type="STRING" id="273057.SSO3052"/>
    <dbReference type="PaxDb" id="273057-SSO3052"/>
    <dbReference type="EnsemblBacteria" id="AAK43152">
        <property type="protein sequence ID" value="AAK43152"/>
        <property type="gene ID" value="SSO3052"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3052"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3150"/>
    <dbReference type="eggNOG" id="arCOG05121">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051989_5_2_2"/>
    <dbReference type="InParanoid" id="Q97UG8"/>
    <dbReference type="PhylomeDB" id="Q97UG8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01834">
        <property type="entry name" value="SGNH_hydrolase_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051532">
        <property type="entry name" value="Ester_Hydrolysis_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30383:SF5">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30383">
        <property type="entry name" value="THIOESTERASE 1/PROTEASE 1/LYSOPHOSPHOLIPASE L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="210" mass="24122" checksum="17D8B1EC6377B785" modified="2001-10-01" version="1">MRLSNGDVIVFAGDSITDREKSNNAPLGYGYVNLFYNLMLAKHPETKITVINSGISGNTVIDLIDRWDDDVLSYKPNWISILIGINDLHKFLGGLTTFNPENYYENLRRLLKYTRKVLNNVNFILMTPFYISRNKLEGGFRAKVLELLPQYIEKVRLLSNEFSATYIDLHDTFKKLVNIREPNVYAPEPVHPNFTGHMVIALKLLEALEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UL4</accession>
    <name>Q97UL4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2067 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90479">
        <property type="entry name" value="H90479"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992642.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UL4"/>
    <dbReference type="PaxDb" id="273057-SSO2990"/>
    <dbReference type="EnsemblBacteria" id="AAK43095">
        <property type="protein sequence ID" value="AAK43095"/>
        <property type="gene ID" value="SSO2990"/>
    </dbReference>
    <dbReference type="GeneID" id="1453014"/>
    <dbReference type="KEGG" id="sso:SSO2990"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3084"/>
    <dbReference type="eggNOG" id="arCOG05968">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1222575_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UL4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="26848" checksum="35814B3199C0E0EE" modified="2001-10-01" version="1">MEKVIIDKYIIRTDCSDDNVLNDLVKILRKYNIKAYNYKVEFLHNKVSIRAIRRNIILNLSNLYIKDMEDILEESEELYTTRFGIEFHNIPSKREILDKLEATKLPYSKVDVFKDYVRIWTINGFTFIDGKSLEATYYLSLILEKVNLEPFNLGRIRKVKDMRALLLLKYYGIRDLDLIEKLIDLGLRIENDNEIIIDNISISKKGIFKKGNEVSKKELYELVKVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UM1</accession>
    <name>Q97UM1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90478">
        <property type="entry name" value="H90478"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992582.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UM1"/>
    <dbReference type="STRING" id="273057.SSO2982"/>
    <dbReference type="PaxDb" id="273057-SSO2982"/>
    <dbReference type="EnsemblBacteria" id="AAK43087">
        <property type="protein sequence ID" value="AAK43087"/>
        <property type="gene ID" value="SSO2982"/>
    </dbReference>
    <dbReference type="GeneID" id="1453006"/>
    <dbReference type="KEGG" id="sso:SSO2982"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3074"/>
    <dbReference type="eggNOG" id="arCOG06035">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_867723_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UM1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="320" mass="37061" checksum="357BDC67394E4EB6" modified="2001-10-01" version="1">MKEIKEIEPWGVNIPFIFLAMIYWALGSLSLPLNLPFHPYFMLLGAYALYFGMIQRLFFPAKNYLALHIASLILLAIPIQYFQIIASVVLTITEVWALKDLKMYGYNTKKLPINALVLSSPFSSIIAWVFYPNYWLLITPLLLYILGVNVGVFSVNLRTKPVFGLHQLPIFLIIILSYFFPILFPFIGVVYFLTIYRKTFTFKSITGISSLLSLIVIPLLSLYFGDFVHAFTLGIMSNLFFSCITYSTSRYNYNKVVISILLSDLAYILRFFYFEISGIFWIVAVIYFLYLIKDNFYLTSIKLGLSMRFIRMQKENRGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UP0</accession>
    <name>Q97UP0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF929 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2964</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90476">
        <property type="entry name" value="E90476"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924074.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UP0"/>
    <dbReference type="STRING" id="273057.SSO2964"/>
    <dbReference type="PaxDb" id="273057-SSO2964"/>
    <dbReference type="EnsemblBacteria" id="AAK43068">
        <property type="protein sequence ID" value="AAK43068"/>
        <property type="gene ID" value="SSO2964"/>
    </dbReference>
    <dbReference type="GeneID" id="1452987"/>
    <dbReference type="KEGG" id="sso:SSO2964"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3054"/>
    <dbReference type="eggNOG" id="arCOG03843">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090239_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UP0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009272">
        <property type="entry name" value="DUF929"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06053">
        <property type="entry name" value="DUF929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="28092" checksum="BC3A972C9A576AF8" modified="2001-10-01" version="1">MNRRNLITAIIGIIVIATGIIVYANHFIASAQPIPAGKFVKISNIDLAPKGKVIIVEQSWYGCPVGAAASWAIYDVLQHYGNLSYELHYSDPDHNPANIPGLIFTGFNSTSVVEFYVAYVYNEYLNASYNGTLIPQNELISVGEQILEEEIAKMGLSPQVAQYIIQYETEVAIQQYGEPSALYVKPPHLNFAILISGPNGTYIVTTPIVNPTILEGYTPSYVLNNLDQFPQIVQAAQMIQNTILEAAGPLAGECPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UP6</accession>
    <name>Q97UP6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1395</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2958</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90475">
        <property type="entry name" value="G90475"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UP6"/>
    <dbReference type="STRING" id="273057.SSO2958"/>
    <dbReference type="PaxDb" id="273057-SSO2958"/>
    <dbReference type="EnsemblBacteria" id="AAK43062">
        <property type="protein sequence ID" value="AAK43062"/>
        <property type="gene ID" value="SSO2958"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2958"/>
    <dbReference type="eggNOG" id="arCOG02129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2056158_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UP6"/>
    <dbReference type="PhylomeDB" id="Q97UP6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="15"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="135" mass="15242" checksum="3F15FD68E32AF518" modified="2001-10-01" version="1">MRERLKGVIKKGVKVFFTDESGIHHDPSKVRRLGLYNVPVDYPSRKLNVLASIPLFDGVPCFMSTYSNVNFKIFAEFLRLFRNSGPIVLILDSAKFHKNAYVFSVASQLGITLLFLPPYSPDLNPIEFGGTWFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UQ1</accession>
    <name>Q97UQ1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2955</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90475">
        <property type="entry name" value="B90475"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UQ1"/>
    <dbReference type="PaxDb" id="273057-SSO2955"/>
    <dbReference type="EnsemblBacteria" id="AAK43057">
        <property type="protein sequence ID" value="AAK43057"/>
        <property type="gene ID" value="SSO2955"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2955"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3044"/>
    <dbReference type="eggNOG" id="arCOG00132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_711011_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UQ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23513">
        <property type="entry name" value="INTEGRAL MEMBRANE EFFLUX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23513:SF6">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="388" mass="43930" checksum="5538066FBF0D8756" modified="2001-10-01" version="1">MYVLVSSPFHYKQFTKFTIFSVFAGFSFIQNELTTYWFFILLFRQEITLFGLGVIARPLVRVFVSYLMGYISDKYDRAKLFYITRGISGILLFPLTFAFIYHSIPLIFGIYYIRTIIVELSNNVGYVAYYAVVPEQVRAKAIFYIRIISMASRVASGAVWYLIYQLISAYDLFLAGILGLIGLALLKGFNIGGGSKRVKLSTAIDFFRKDERIRGIILLYSVTDGLTYSINYLLPLLIIVYHGTEEIYSLSQTVLYGILVIASFVMTRIKDPIKIVSTYILSGFLFYLVLLYPSPYVLLISVLLYGFGSGIVENYVMATIKQSVGDEFLGSVLGLDVLVTSSVEIFLILLGQYLITINVVYYIIVGIVGMSLVLISWLLHPKLKDIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UT3</accession>
    <name>Q97UT3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Adenosine 5'-phosphosulfate reductase</fullName>
            <ecNumber evidence="9">1.8.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">5'-adenylylsulfate reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Thioredoxin-dependent 5'-adenylylsulfate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">cysH</name>
        <name evidence="15" type="ordered locus">SSO2911</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of sulfite from adenosine 5'-phosphosulfate (APS) using thioredoxin as an electron donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>[thioredoxin]-disulfide + sulfite + AMP + 2 H(+) = adenosine 5'-phosphosulfate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:21976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="1.8.4.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.10" evidence="9"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90470">
        <property type="entry name" value="D90470"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993313.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UT3"/>
    <dbReference type="FunCoup" id="Q97UT3">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2911"/>
    <dbReference type="PaxDb" id="273057-SSO2911"/>
    <dbReference type="EnsemblBacteria" id="AAK43019">
        <property type="protein sequence ID" value="AAK43019"/>
        <property type="gene ID" value="SSO2911"/>
    </dbReference>
    <dbReference type="GeneID" id="1452938"/>
    <dbReference type="KEGG" id="sso:SSO2911"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3000"/>
    <dbReference type="eggNOG" id="arCOG00124">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044089_1_0_2"/>
    <dbReference type="InParanoid" id="Q97UT3"/>
    <dbReference type="PhylomeDB" id="Q97UT3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043866">
        <property type="term" value="F:adenylyl-sulfate reductase (thioredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004604">
        <property type="term" value="F:phosphoadenylyl-sulfate reductase (thioredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019379">
        <property type="term" value="P:sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23945">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00063">
        <property type="entry name" value="CysH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011798">
        <property type="entry name" value="APS_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004511">
        <property type="entry name" value="PAPS/APS_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02055">
        <property type="entry name" value="APS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00434">
        <property type="entry name" value="cysH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002537">
        <property type="entry name" value="PRK02090.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46482:SF9">
        <property type="entry name" value="5'-ADENYLYLSULFATE REDUCTASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46482">
        <property type="entry name" value="5'-ADENYLYLSULFATE REDUCTASE 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000857">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="14">
        <location>
            <begin position="34"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAK43019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="239" mass="28461" checksum="8356E07BE3E7302D" modified="2001-10-01" version="1">MLSKDELDSLNKLFEDKEPLEVLKWGIEKFYPKIALACSLQAEDLVILDMLSKVTEKPRVFIIDTGRLHQESYDLIEEIVKKYNIDLRIYFPDYKEVEDLVNKYGINLFYKSVELRKACCEVRKVRPLKRALDGMEAWITGLRREQNFTRGRIRKIEIDEVNGGIIKLNPLADWTWEQVWEYINKNNVPYNKLYDKGYKSIGCVPCTRPVKPWEHPRAGRWWWEQNSDKECGLHYREVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UX4</accession>
    <name>Q97UX4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2867</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90464">
        <property type="entry name" value="G90464"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UX4"/>
    <dbReference type="STRING" id="273057.SSO2867"/>
    <dbReference type="PaxDb" id="273057-SSO2867"/>
    <dbReference type="EnsemblBacteria" id="AAK42974">
        <property type="protein sequence ID" value="AAK42974"/>
        <property type="gene ID" value="SSO2867"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2867"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2953"/>
    <dbReference type="eggNOG" id="arCOG01668">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138323_1_0_2"/>
    <dbReference type="InParanoid" id="Q97UX4"/>
    <dbReference type="PhylomeDB" id="Q97UX4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="117" mass="13365" checksum="6426AA8F52EBF1B4" modified="2001-10-01" version="1">MILKDENMAKVLFLIMSGDEKFDLAMRMAYNSVKNKRYEDVKVIYFGPSQKKLTQLDGDMKNMFQELLQNKIVDSACIGVAQAMNIKPHLENLGVSLMPAGERVSYYVNQGYEVITF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2019-07-03" modified="2025-06-18" version="156" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UY8</accession>
    <name>GLCV_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glucose import ATP-binding protein GlcV</fullName>
            <ecNumber evidence="7">7.5.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">glcV</name>
        <name evidence="10" type="ordered locus">SSO2850</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="J. Bacteriol." volume="181" first="4285" last="4291">
            <title>Glucose transport in the extremely thermoacidophilic Sulfolobus solfataricus involves a high-affinity membrane-integrated binding protein.</title>
            <authorList>
                <person name="Albers S.V."/>
                <person name="Elferink M.G."/>
                <person name="Charlebois R.L."/>
                <person name="Sensen C.W."/>
                <person name="Driessen A.J."/>
                <person name="Konings W.N."/>
            </authorList>
            <dbReference type="PubMed" id="10400586"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Mol. Microbiol." volume="39" first="1494" last="1503">
            <title>Sugar transport in Sulfolobus solfataricus is mediated by two families of binding protein-dependent ABC transporters.</title>
            <authorList>
                <person name="Elferink M.G."/>
                <person name="Albers S.V."/>
                <person name="Konings W.N."/>
                <person name="Driessen A.J."/>
            </authorList>
            <dbReference type="PubMed" id="11260467"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2002" name="Acta Crystallogr. D" volume="58" first="362" last="365">
            <title>Purification, crystallization and preliminary X-ray diffraction analysis of an archaeal ABC-ATPase.</title>
            <authorList>
                <person name="Verdon G."/>
                <person name="Albers S.V."/>
                <person name="Dijkstra B.W."/>
                <person name="Driessen A.J."/>
                <person name="Thunnissen A.M."/>
            </authorList>
            <dbReference type="PubMed" id="11807278"/>
        </citation>
        <scope>ATPASE ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>CRYSTALLIZATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="13 14 15" key="5">
        <citation type="journal article" date="2003" name="J. Mol. Biol." volume="330" first="343" last="358">
            <title>Crystal structures of the ATPase subunit of the glucose ABC transporter from Sulfolobus solfataricus: nucleotide-free and nucleotide-bound conformations.</title>
            <authorList>
                <person name="Verdon G."/>
                <person name="Albers S.V."/>
                <person name="Dijkstra B.W."/>
                <person name="Driessen A.J."/>
                <person name="Thunnissen A.M."/>
            </authorList>
            <dbReference type="PubMed" id="12823973"/>
            <dbReference type="DOI" id="10.1016/S0022-2836(03)00575-8"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF APOPROTEIN AND IN COMPLEXES WITH ADP AND ATP ANALOG</scope>
        <scope>FUNCTION</scope>
        <scope>DOMAIN</scope>
    </reference>
    <reference evidence="16" key="6">
        <citation type="journal article" date="2003" name="J. Mol. Biol." volume="334" first="255" last="267">
            <title>Formation of the productive ATP-Mg2+-bound dimer of GlcV, an ABC-ATPase from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Verdon G."/>
                <person name="Albers S.V."/>
                <person name="van Oosterwijk N."/>
                <person name="Dijkstra B.W."/>
                <person name="Driessen A.J."/>
                <person name="Thunnissen A.M."/>
            </authorList>
            <dbReference type="PubMed" id="14607117"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2003.08.065"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF MUTANT ALA-144</scope>
        <scope>FUNCTION</scope>
        <scope>ATPASE ACTIVITY</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF SER-142; GLY-144 AND GLU-166</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3 4 7 8">Part of the ABC transporter complex GlcSTUV involved in glucose uptake (Probable). Responsible for energy coupling to the transport system (PubMed:12823973, PubMed:14607117). In vitro, as a free subunit, exhibits a constitutive ATPase activity (PubMed:11807278).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>D-glucose(out) + ATP + H2O = D-glucose(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60184"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:60185"/>
        </physiologicalReaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">290 uM for ATP</KM>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 6.5 for ATPase activity.</text>
        </phDependence>
    </comment>
    <comment type="subunit">
        <text evidence="4 7">The complex is composed of two ATP-binding proteins (GlcV), two transmembrane proteins (GlcT and GlcU) and a solute-binding protein (GlcS) (Probable). Forms transient head-to-tail homodimers in the presence of ATP-Mg(2+) (PubMed:14607117).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">Consists of a typical ABC-ATPase domain, comprising two subdomains, connected by a linker region to a C-terminal domain of unknown function.</text>
    </comment>
    <comment type="similarity">
        <text>Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="7.5.2.-" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90463">
        <property type="entry name" value="A90463"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924001.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1OXS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.65 A"/>
        <property type="chains" value="C=1-353"/>
    </dbReference>
    <dbReference type="PDB" id="1OXT">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B/D=1-353"/>
    </dbReference>
    <dbReference type="PDB" id="1OXU">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B/C=1-353"/>
    </dbReference>
    <dbReference type="PDB" id="1OXV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.95 A"/>
        <property type="chains" value="A/B/D=1-353"/>
    </dbReference>
    <dbReference type="PDB" id="1OXX">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.45 A"/>
        <property type="chains" value="K=1-353"/>
    </dbReference>
    <dbReference type="PDBsum" id="1OXS"/>
    <dbReference type="PDBsum" id="1OXT"/>
    <dbReference type="PDBsum" id="1OXU"/>
    <dbReference type="PDBsum" id="1OXV"/>
    <dbReference type="PDBsum" id="1OXX"/>
    <dbReference type="AlphaFoldDB" id="Q97UY8"/>
    <dbReference type="SMR" id="Q97UY8"/>
    <dbReference type="FunCoup" id="Q97UY8">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2850"/>
    <dbReference type="TCDB" id="3.A.1.1.13">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2850"/>
    <dbReference type="EnsemblBacteria" id="AAK42960">
        <property type="protein sequence ID" value="AAK42960"/>
        <property type="gene ID" value="SSO2850"/>
    </dbReference>
    <dbReference type="GeneID" id="1452879"/>
    <dbReference type="GeneID" id="27429116"/>
    <dbReference type="KEGG" id="sso:SSO2850"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2936"/>
    <dbReference type="eggNOG" id="arCOG00175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_2"/>
    <dbReference type="InParanoid" id="Q97UY8"/>
    <dbReference type="PhylomeDB" id="Q97UY8"/>
    <dbReference type="BRENDA" id="7.5.2.B8">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97UY8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055052">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015408">
        <property type="term" value="F:ABC-type ferric iron transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03259">
        <property type="entry name" value="ABC_Carb_Solutes_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000042">
        <property type="entry name" value="Maltose/maltodextrin ABC transporter, ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053598">
        <property type="entry name" value="ABC-Glucose_import_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015853">
        <property type="entry name" value="ABC_transpr_FbpC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047641">
        <property type="entry name" value="ABC_transpr_MalK/UgpC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040856">
        <property type="entry name" value="GlcV_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040933">
        <property type="entry name" value="ABC_arch_GlcV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875:SF4">
        <property type="entry name" value="GLUCOSE IMPORT ATP-BINDING PROTEIN GLCV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875">
        <property type="entry name" value="MALTODEXTRIN IMPORT ATP-BINDING PROTEIN MSMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17847">
        <property type="entry name" value="GlcV_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="MOP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0762">Sugar transport</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000447615" description="Glucose import ATP-binding protein GlcV">
        <location>
            <begin position="1"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="4"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="40"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Decrease in ATPase activity. Can form dimers." evidence="4">
        <original>S</original>
        <variation>A</variation>
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of ATPase activity. Cannot form dimers. Forms an active heterodimer; when associated with A-166." evidence="4">
        <original>G</original>
        <variation>A</variation>
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of ATPase activity. Can form dimers in the presence of ATP-Mg(2+). Forms an active heterodimer; when associated with A-144." evidence="4">
        <original>E</original>
        <variation>A</variation>
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Strong decrease in ATPase activity. Can form dimers in the presence of ATP alone, without Mg(2+)." evidence="4">
        <original>E</original>
        <variation>Q</variation>
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="4"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="helix" evidence="17">
        <location>
            <begin position="14"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="33"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="helix" evidence="17">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="58"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="67"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="74"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="helix" evidence="17">
        <location>
            <begin position="79"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="84"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="helix" evidence="17">
        <location>
            <begin position="99"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="helix" evidence="17">
        <location>
            <begin position="105"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="helix" evidence="17">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="helix" evidence="17">
        <location>
            <begin position="131"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="helix" evidence="17">
        <location>
            <begin position="138"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="helix" evidence="17">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="160"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="turn" evidence="17">
        <location>
            <begin position="167"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="helix" evidence="17">
        <location>
            <begin position="173"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="helix" evidence="17">
        <location>
            <begin position="176"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="193"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="helix" evidence="17">
        <location>
            <begin position="201"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="209"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="218"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="helix" evidence="17">
        <location>
            <begin position="225"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="helix" evidence="17">
        <location>
            <begin position="235"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="245"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="255"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="261"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="268"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="helix" evidence="17">
        <location>
            <begin position="278"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="281"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="293"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="310"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="324"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="337"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="helix" evidence="17">
        <location>
            <begin position="344"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="strand" evidence="17">
        <location>
            <begin position="348"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11807278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12823973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="14607117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="11260467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <evidence type="ECO:0000305" key="7">
        <source>
            <dbReference type="PubMed" id="10400586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="8">
        <source>
            <dbReference type="PubMed" id="11260467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="9">
        <source>
            <dbReference type="PubMed" id="12823973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK42960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="13">
        <source>
            <dbReference type="PDB" id="1OXS"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="14">
        <source>
            <dbReference type="PDB" id="1OXT"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="15">
        <source>
            <dbReference type="PDB" id="1OXU"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="16">
        <source>
            <dbReference type="PDB" id="1OXX"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="17">
        <source>
            <dbReference type="PDB" id="1OXX"/>
        </source>
    </evidence>
    <sequence length="353" mass="39113" checksum="772D42F471DCD980" modified="2001-10-01" version="1">MVRIIVKNVSKVFKKGKVVALDNVNINIENGERFGILGPSGAGKTTFMRIIAGLDVPSTGELYFDDRLVASNGKLIVPPEDRKIGMVFQTWALYPNLTAFENIAFPLTNMKMSKEEIRKRVEEVAKILDIHHVLNHFPRELSGGQQQRVALARALVKDPSLLLLDEPFSNLDARMRDSARALVKEVQSRLGVTLLVVSHDPADIFAIADRVGVLVKGKLVQVGKPEDLYDNPVSIQVASLIGEINELEGKVTNEGVVIGSLRFPVSVSSDRAIIGIRPEDVKLSKDVIKDDSWILVGKGKVKVIGYQGGLFRITITPLDSEEEIFTYSDHPIHSGEEVLVYVRKDKIKVFEKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-05-30" modified="2024-03-27" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V15</accession>
    <name>FDHD_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sulfur carrier protein FdhD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fdhD</name>
        <name type="ordered locus">SSO2821</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for formate dehydrogenase (FDH) activity. Acts as a sulfur carrier protein that transfers sulfur from IscS to the molybdenum cofactor prior to its insertion into FDH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FdhD family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90459">
        <property type="entry name" value="E90459"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V15"/>
    <dbReference type="SMR" id="Q97V15"/>
    <dbReference type="STRING" id="273057.SSO2821"/>
    <dbReference type="PaxDb" id="273057-SSO2821"/>
    <dbReference type="EnsemblBacteria" id="AAK42932">
        <property type="protein sequence ID" value="AAK42932"/>
        <property type="gene ID" value="SSO2821"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2821"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2907"/>
    <dbReference type="eggNOG" id="arCOG04358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056887_3_0_2"/>
    <dbReference type="InParanoid" id="Q97V15"/>
    <dbReference type="PhylomeDB" id="Q97V15"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097163">
        <property type="term" value="F:sulfur carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00187">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003786">
        <property type="entry name" value="FdhD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00129">
        <property type="entry name" value="fdhD_narQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30592">
        <property type="entry name" value="FORMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30592:SF1">
        <property type="entry name" value="SULFUR CARRIER PROTEIN FDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02634">
        <property type="entry name" value="FdhD-NarQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015626">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152940" description="Sulfur carrier protein FdhD">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="1">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00187"/>
        </source>
    </evidence>
    <sequence length="257" mass="28711" checksum="89A6444768070D5C" modified="2001-10-01" version="1">MGVRVKRFDLVRVKDSEAYKDSDFVAVEEPLNIRTCFEKCEDFAIIMRTPGDDKELSLGFLYSEGVINSIHDVEDIKQVENNVIEVKLNKPIGIRIRELIVNSSCGVCGRAFLYTLNILKSDLKVKREVIFSFPEKLREKQSVFNISGGLHATALFNPQGELLFIYEDVGRHNAVDKVVGRLLLEDKIPASNYIMQVSGRLGYEIVSKGIKAGIPIICGISAPTSLAIEIAEEAGLTLIGFLRGKSLNIYTHSERIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-11-27" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V37</accession>
    <name>Q97V37_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oxydoreductase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2795</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90456">
        <property type="entry name" value="E90456"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923973.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2795"/>
    <dbReference type="PaxDb" id="273057-SSO2795"/>
    <dbReference type="EnsemblBacteria" id="AAK42908">
        <property type="protein sequence ID" value="AAK42908"/>
        <property type="gene ID" value="SSO2795"/>
    </dbReference>
    <dbReference type="GeneID" id="1452827"/>
    <dbReference type="KEGG" id="sso:SSO2795"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2881"/>
    <dbReference type="eggNOG" id="arCOG01496">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_252241_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V37"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02783">
        <property type="entry name" value="MopB_CT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.228.10">
        <property type="entry name" value="Dimethylsulfoxide Reductase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019546">
        <property type="entry name" value="TAT_signal_bac_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01409">
        <property type="entry name" value="TAT_signal_seq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43598:SF5">
        <property type="entry name" value="DMSO REDUCTASE CHAIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43598">
        <property type="entry name" value="TUNGSTEN-CONTAINING FORMYLMETHANOFURAN DEHYDROGENASE 2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10518">
        <property type="entry name" value="TAT_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="1426" mass="157871" checksum="106D5C745356F508" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V41</accession>
    <name>Q97V41_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HAD family hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2791</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99456">
        <property type="entry name" value="A99456"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V41"/>
    <dbReference type="SMR" id="Q97V41"/>
    <dbReference type="STRING" id="273057.SSO2791"/>
    <dbReference type="PaxDb" id="273057-SSO2791"/>
    <dbReference type="EnsemblBacteria" id="AAK42904">
        <property type="protein sequence ID" value="AAK42904"/>
        <property type="gene ID" value="SSO2791"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2791"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2877"/>
    <dbReference type="eggNOG" id="arCOG06026">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1425144_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V41"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="22283" checksum="9FA96B63EEAC0CA7" modified="2001-10-01" version="1">MGKMIPYVIDLDAIFDLSSIEIYRLLTSSLSNVRYYPPKDLLKTVINDFDISKFKPFPDSQYLEELNYRARIYVITNLPKIEAKLLLVRYNLDAFIQDVISPEDINRYFPSKEVLVLISRKTNSPLGSITFITPHIDYAILTSNLGMRTILIRNSISLPRDIRVEIRKSLVELAEITAQHKIDSEAGDSALRCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V76</accession>
    <name>Q97V76_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">pyruvate synthase</fullName>
            <ecNumber evidence="1">1.2.7.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">porG-2</name>
        <name evidence="5" type="ordered locus">SSO2758</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2 oxidized [2Fe-2S]-[ferredoxin] + pyruvate + CoA = 2 reduced [2Fe-2S]-[ferredoxin] + acetyl-CoA + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12765"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="1.2.7.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.2.7.1" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90451">
        <property type="entry name" value="F90451"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990249.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V76"/>
    <dbReference type="FunCoup" id="Q97V76">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2758"/>
    <dbReference type="PaxDb" id="273057-SSO2758"/>
    <dbReference type="EnsemblBacteria" id="AAK42869">
        <property type="protein sequence ID" value="AAK42869"/>
        <property type="gene ID" value="SSO2758"/>
    </dbReference>
    <dbReference type="GeneID" id="1452788"/>
    <dbReference type="KEGG" id="sso:SSO2758"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2838"/>
    <dbReference type="eggNOG" id="arCOG01603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087284_2_0_2"/>
    <dbReference type="InParanoid" id="Q97V76"/>
    <dbReference type="PhylomeDB" id="Q97V76"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051626">
        <property type="entry name" value="Oxidoreductase_gamma_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43366">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43366:SF1">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="4">
        <location>
            <begin position="10"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00049357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="184" mass="20226" checksum="B5A64C0C3E056FD0" modified="2001-10-01" version="1">MLEIRFHGRGGQGVVTASQLLAEAAGYENLYTSSFPIYGAERRGAEIEAYCRISDTPIRVTSPVEEPDYLVVIDNSLIQISRSLFRGLKDTSTILLNSPSKPDLRWRTFYVNATKIATDLGLVKSGWPMVNVIILGALIRVMGMPKLSSLEKAIEEEFDGKVAQLNIKGARLAYGQVGVEYVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V89</accession>
    <name>Q97V89_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2746</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90450">
        <property type="entry name" value="A90450"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V89"/>
    <dbReference type="STRING" id="273057.SSO2746"/>
    <dbReference type="PaxDb" id="273057-SSO2746"/>
    <dbReference type="EnsemblBacteria" id="AAK42856">
        <property type="protein sequence ID" value="AAK42856"/>
        <property type="gene ID" value="SSO2746"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2746"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2825"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051127_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V89"/>
    <dbReference type="PhylomeDB" id="Q97V89"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518">
        <property type="entry name" value="C-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="239"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="423" mass="47227" checksum="CF02CD4FD8D95ABA" modified="2001-10-01" version="1">MWLKQVMIKYKDTRWMYLIIPYNASTGPLSTLVTLQILSLGGNAINVAYAISLSNAVLIPASILWGFMADRMDRKKQILLSLAGVSIPLVFMPFMNSISLVTLNYALITFMSTASATPFNLLIMESVEKKHWGSLFSRFSLFSSIGVLLGLVISTFLVILLRIDQIEEILGFSVLATLIASIKILPKPIITFERTAIIHHKESFLTRMRHLPMMFLHLPNIHHFKMFSMTRLFRKPINYVPLLYLGIVLFYISSGIFNTVYPAGLYVKGLNKSEVLAVISVGMVFQILGFRISSMLLESKDEKGLAHISLILRGVSYVVLGIFAQLFLGIPIFISGLIFYPLAAGIAYAIFYSSSTTLIFKIVGERRQGTGLGVYSTVVGISLFLGSLLSGYITHYISYGIDFITAGIILLFASWIFRYLEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VA1</accession>
    <name>Q97VA1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90448">
        <property type="entry name" value="E90448"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VA1"/>
    <dbReference type="STRING" id="273057.SSO2734"/>
    <dbReference type="PaxDb" id="273057-SSO2734"/>
    <dbReference type="EnsemblBacteria" id="AAK42844">
        <property type="protein sequence ID" value="AAK42844"/>
        <property type="gene ID" value="SSO2734"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2734"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2813"/>
    <dbReference type="eggNOG" id="arCOG08518">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2476176_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VA1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="101" mass="11711" checksum="D919786557AFF113" modified="2001-10-01" version="1">MNRAYIFLCSFYLLMGVSISDIKYIVERPEIFGFKVETIRKDNGFNTVINDVKQNGIRIDNYWVTCNKDNGECEVVDDNSNSLIIVNFIKKTIIKYVTSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VA7</accession>
    <name>Q97VA7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">4-aminobutyrate aminotransferase (GabT-1)</fullName>
            <ecNumber evidence="5">2.6.1.19</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">gabT-1</name>
        <name evidence="5" type="ordered locus">SSO2727</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.19" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90447">
        <property type="entry name" value="G90447"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991724.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VA7"/>
    <dbReference type="FunCoup" id="Q97VA7">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2727"/>
    <dbReference type="PaxDb" id="273057-SSO2727"/>
    <dbReference type="EnsemblBacteria" id="AAK42838">
        <property type="protein sequence ID" value="AAK42838"/>
        <property type="gene ID" value="SSO2727"/>
    </dbReference>
    <dbReference type="GeneID" id="1452757"/>
    <dbReference type="KEGG" id="sso:SSO2727"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2807"/>
    <dbReference type="eggNOG" id="arCOG00915">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_0_2"/>
    <dbReference type="InParanoid" id="Q97VA7"/>
    <dbReference type="PhylomeDB" id="Q97VA7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034386">
        <property type="term" value="F:4-aminobutyrate:2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000249">
        <property type="entry name" value="Aminotransferase class-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF58">
        <property type="entry name" value="LEUCINE_METHIONINE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="440" mass="48781" checksum="44B2CFDCB62EA857" modified="2001-10-01" version="1">MEENLDSSVLEAPIINVTPPGSKSLKLLKDQEEYETSAINYPKYFKIAIDKAQGSTVTDVDGNVYIDLVTGISVVNLGHNNPFVRKRVQEQLEKVWHTLEVPTEIRVNFSKKLLSTLGMRAKLLFTTTGADAVEAAVKIARFITGKKTIIAFEGSYHGITAGTLGLTGANRFKEFQPFFDDRVVKFPYPYPYRCPFKDCLNETLSLLDYAMSNPGYLGGDVAGILVEPIQGEGGYVVPPKGFLKGLKELAEKYSVPLIVDEVQTGVGRTGKMWAYQWENIEPDIVTISKAIGEGIPVSMVAYREDFDKLPTGFHLGTYRGNPLGLAAGLASLEFIESHNILSRVERLGRKALELLKEVQNPHVGDIRGLGFMIGIELVKDSKEPWSEGTKVVIERALKRGLLVYKAGRWDNVIRLMPPLTIPESLLDRAIEILKIILNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VD4</accession>
    <name>Q97VD4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl-CoA c-acetyltransferase (Acetoacetyl-CoA thiolase) (AcaB-8)</fullName>
            <ecNumber evidence="4">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">acaB-8</name>
        <name evidence="4" type="ordered locus">SSO2697</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.9" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90444">
        <property type="entry name" value="C90444"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988620.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VD4"/>
    <dbReference type="FunCoup" id="Q97VD4">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2697"/>
    <dbReference type="PaxDb" id="273057-SSO2697"/>
    <dbReference type="DNASU" id="1452729"/>
    <dbReference type="EnsemblBacteria" id="AAK42810">
        <property type="protein sequence ID" value="AAK42810"/>
        <property type="gene ID" value="SSO2697"/>
    </dbReference>
    <dbReference type="GeneID" id="1452729"/>
    <dbReference type="KEGG" id="sso:SSO2697"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2777"/>
    <dbReference type="eggNOG" id="arCOG01278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_2"/>
    <dbReference type="InParanoid" id="Q97VD4"/>
    <dbReference type="PhylomeDB" id="Q97VD4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF1">
        <property type="entry name" value="NON-SPECIFIC LIPID-TRANSFER PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="3">
        <location>
            <begin position="231"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="376" mass="40529" checksum="ACC803A737EE8339" modified="2001-10-01" version="1">MVAIIDASITRFGKRKESLLELAGEVALPLIGKYEIDFVVVSNSYSGEFNSLSGINNLITTYLSVDKVPSIRVDNTSGSGGSAILVAKSLLDSKITKTVLVLGVEKMSEKNTKQVTSVIASLLSPEEKIVGLTLPSLAGLMTKEYMRRYNASREAFALVAVKNHYNGSLNPFAHIQKVVSLEEVLNSPIIADPLTLYEFTPISDGASAIVMVRDEDAYSFTKKPVFIKGMGASSDTSYLSEREDILTMNAVRIAGLTARKMAKVDKVDFAELHDMATVLEIVEAEELGLLKKGEGWKAYYDDYTKINGEMPINTSGGLNSKGHPIGASGIAQAVEAFLQIRNQAGSRQVRNARVGLSLSMAGFGNSATVIIYGDEP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-20" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VE1</accession>
    <name>Y2687_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0248 protein SSO2687</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2687</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0248 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK42803" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42803.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90443">
        <property type="entry name" value="D90443"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988607.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VE1"/>
    <dbReference type="STRING" id="273057.SSO2687"/>
    <dbReference type="PaxDb" id="273057-SSO2687"/>
    <dbReference type="EnsemblBacteria" id="AAK42803">
        <property type="protein sequence ID" value="AAK42803"/>
        <property type="gene ID" value="SSO2687"/>
    </dbReference>
    <dbReference type="GeneID" id="1452722"/>
    <dbReference type="KEGG" id="sso:SSO2687"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2767"/>
    <dbReference type="eggNOG" id="arCOG01302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172276_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VE1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01245">
        <property type="entry name" value="UPF0248"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040459">
        <property type="entry name" value="MJ1316"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007547">
        <property type="entry name" value="UPF0248"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04457">
        <property type="entry name" value="MJ1316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000053422" description="UPF0248 protein SSO2687">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="81" mass="9742" checksum="3209C4C36011C305" modified="2003-06-20" version="2">MKIKDAINRVRWKYKEKIDDYVIVIKDKLTETGLKEIPFTDIYTVDNNYLYLKGEDTIIPLHRVLMIRRKSDDALIWKRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VE4</accession>
    <name>Q97VE4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2683</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90443">
        <property type="entry name" value="A90443"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988602.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VE4"/>
    <dbReference type="STRING" id="273057.SSO2683"/>
    <dbReference type="PaxDb" id="273057-SSO2683"/>
    <dbReference type="EnsemblBacteria" id="AAK42800">
        <property type="protein sequence ID" value="AAK42800"/>
        <property type="gene ID" value="SSO2683"/>
    </dbReference>
    <dbReference type="GeneID" id="1452719"/>
    <dbReference type="KEGG" id="sso:SSO2683"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2764"/>
    <dbReference type="eggNOG" id="arCOG08522">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1943952_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VE4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="129" mass="13949" checksum="F1F7166B0C6AB591" modified="2001-10-01" version="1">MKGQASVIAMVVIFFLIIATIGLILYISVSYENLQKEYIQVSQILANKAKENLLIGYVNSSLSIYNAGSVVTTIVAILLINKTNVTIVPENIVIPPNSNVIIHVKTANGYVIVTSYGNSFYIAPSSGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VJ1</accession>
    <name>Q97VJ1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2632</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90437">
        <property type="entry name" value="B90437"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VJ1"/>
    <dbReference type="STRING" id="273057.SSO2632"/>
    <dbReference type="PaxDb" id="273057-SSO2632"/>
    <dbReference type="EnsemblBacteria" id="AAK42753">
        <property type="protein sequence ID" value="AAK42753"/>
        <property type="gene ID" value="SSO2632"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2632"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2715"/>
    <dbReference type="eggNOG" id="arCOG02114">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062162_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VJ1"/>
    <dbReference type="PhylomeDB" id="Q97VJ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012440">
        <property type="entry name" value="DUF1641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017011">
        <property type="entry name" value="UCP032888"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38433">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38433:SF1">
        <property type="entry name" value="DUF1641 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07849">
        <property type="entry name" value="DUF1641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032888">
        <property type="entry name" value="UCP032888"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="42230" checksum="FA99459F7EBE75FF" modified="2001-10-01" version="1">MVNMQTINLEKLASKIDEKKIDELAELMDLTPALNEALKKVNELKESGALDAIVNSAYVVKTFRDMLNDEAIQSLGNIVSSLLEFGKAISKPKIFENTMAIMDNSDVLADFVNKLKMLKEDGTLDVLINMAYTARTLRDMLNDEAIQNLASTVSSSLEVMKLITQKAEPVKALLDKSSVINDLLVRLEDMKNDGTLDILMNSAYVVKTFRDMLNDEAIQSLGRYISNSLEIMREIDDETLKSIKSTMKKMRLIENVLTKVEELDKNGALDVAFDMAYVAKTLRDMLNDEAITHLSSYVSQFLEVYPKALDFFEIAFSNVPYRMMRAIASEEVKKTLESPPKVSLGGIVRLLSDPEIQRGLGVIFTVIRAIGKEFSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VL3</accession>
    <name>Q97VL3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90434">
        <property type="entry name" value="D90434"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989329.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VL3"/>
    <dbReference type="STRING" id="273057.SSO2610"/>
    <dbReference type="PaxDb" id="273057-SSO2610"/>
    <dbReference type="EnsemblBacteria" id="AAK42731">
        <property type="protein sequence ID" value="AAK42731"/>
        <property type="gene ID" value="SSO2610"/>
    </dbReference>
    <dbReference type="GeneID" id="1454058"/>
    <dbReference type="KEGG" id="sso:SSO2610"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2691"/>
    <dbReference type="eggNOG" id="arCOG05127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104927_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VL3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044036">
        <property type="entry name" value="DUF5752"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19027">
        <property type="entry name" value="DUF5752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="218" mass="25586" checksum="A542AAD172118CED" modified="2001-10-01" version="1">MMDLDSKGKGIPFIFYAAYYPPLYSKLKAISLRELVEGIRKADKYTLFYHVFHPIFSSHLIPEEYSNDFAHWIAESLGDKELAELVSDIPGAEPRTIEDIRNDLIEILEPRINERRGIREFVFVSCTPIVYKTNYVANTLAEFLDLIQIIPDRALVWHFVSKRILGISKRNDFSEWLESNFGLSELAENLSKIDPQTYVYEEVLRRDIIRILERWLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VL6</accession>
    <name>Q97VL6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2607</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99434">
        <property type="entry name" value="A99434"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989326.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VL6"/>
    <dbReference type="PaxDb" id="273057-SSO2607"/>
    <dbReference type="EnsemblBacteria" id="AAK42728">
        <property type="protein sequence ID" value="AAK42728"/>
        <property type="gene ID" value="SSO2607"/>
    </dbReference>
    <dbReference type="GeneID" id="1454055"/>
    <dbReference type="KEGG" id="sso:SSO2607"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2688"/>
    <dbReference type="eggNOG" id="arCOG08528">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1406033_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VL6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="193" mass="22172" checksum="D33A2B0307BEF13A" modified="2001-10-01" version="1">MTLQSRPWRIRSDIWEKLDEICIKLDNKKSYNCTDKIIQSASELIMEDIDITNPSTRNAVLGIKYLIASNLFYNLISSIHDEKIYKEIANSLLSIITNEAQDTGLLLLESFESNCKERSILENVLRGLAYILGDNIKLVADGKTKFEMINVSTNPNIIKFLRIITETLKENFQIIELDESCDNDRICVKLDFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VR8</accession>
    <name>Q97VR8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90427">
        <property type="entry name" value="B90427"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VR8"/>
    <dbReference type="STRING" id="273057.SSO2544"/>
    <dbReference type="PaxDb" id="273057-SSO2544"/>
    <dbReference type="EnsemblBacteria" id="AAK42673">
        <property type="protein sequence ID" value="AAK42673"/>
        <property type="gene ID" value="SSO2544"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2544"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2624"/>
    <dbReference type="HOGENOM" id="CLU_1521944_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VR8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="176" mass="19198" checksum="F69A4256BF868C9C" modified="2001-10-01" version="1">MTIALTVTVSPLESINVSEIVEPTRPLNVVLMLPIEIVIVYVFTPALMVVIPDTLPEFPFISTPVTCVDAPVFFKVTVRVKVLALNVAVPTCVTVPCPVNAPNSPKANPTTTKAIITDAITNINAVTASDNPLAFFLLLAISIPNTLYIIVLYKSFFTPCFVDCFRIVQKVYKLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VX3</accession>
    <name>Q97VX3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thermopsin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2481</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99420">
        <property type="entry name" value="B99420"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923885.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VX3"/>
    <dbReference type="PaxDb" id="273057-SSO2481"/>
    <dbReference type="EnsemblBacteria" id="AAK42617">
        <property type="protein sequence ID" value="AAK42617"/>
        <property type="gene ID" value="SSO2481"/>
    </dbReference>
    <dbReference type="GeneID" id="7808236"/>
    <dbReference type="KEGG" id="sso:SSO2481"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2562"/>
    <dbReference type="eggNOG" id="arCOG07210">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_800814_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VX3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="38876" checksum="D08FC4CE66CA06BB" modified="2001-10-01" version="1">MKRYLILFLVLTSIIFIPITYSSNQFSFLNIGNTISYNIYETSSRLGTYLTFNISMNLVWNGTVFVINGKSVNKVFPAINTNNLTIENISGSYPYPLSVWINTAAAGTPSGFLETGKEFTTYNGIPAVEFLDYNNYTYISLQYMIPLKSYYSINIDPASNLTYSATATLTKGDLNFYTGPYNLYNVSFNYVYQNYTVNLFLLVASSNAEINLLNYSNHVALNITGLKFISLLVPITTFHDLLGVSKFIYNGSPYIIILTSTGSTGSAYYYNTSVPIIGSYIPGTNYYMISIPYSGNIVIIFGNYYSNYVNGSINIKNNDVGHGFSLLYALSAVIVVAVVITATYMLRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VY7</accession>
    <name>Q97VY7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Biotin carboxyl carrier protein of propionyl-CoA carboxylase beta subunit</fullName>
            <ecNumber evidence="3">6.4.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2464</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.3" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90418">
        <property type="entry name" value="D90418"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VY7"/>
    <dbReference type="SMR" id="Q97VY7"/>
    <dbReference type="FunCoup" id="Q97VY7">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2464"/>
    <dbReference type="PaxDb" id="273057-SSO2464"/>
    <dbReference type="EnsemblBacteria" id="AAK42603">
        <property type="protein sequence ID" value="AAK42603"/>
        <property type="gene ID" value="SSO2464"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2464"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2543"/>
    <dbReference type="eggNOG" id="arCOG02699">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_5_4_2"/>
    <dbReference type="InParanoid" id="Q97VY7"/>
    <dbReference type="PhylomeDB" id="Q97VY7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050709">
        <property type="entry name" value="Biotin_Carboxyl_Carrier/Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45266">
        <property type="entry name" value="OXALOACETATE DECARBOXYLASE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45266:SF3">
        <property type="entry name" value="OXALOACETATE DECARBOXYLASE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0092">Biotin</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="2">
        <location>
            <begin position="108"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="186" mass="21167" checksum="DB26587C39883B08" modified="2001-10-01" version="1">MENLWYHIPILSKGVVVMRLFRLYSELGDMYLVSYEQSNNSLDKIKIGDKNYEVKYLGSGNRENEYLFEINGKKYYVFIESDGTLIFNHQDFLRLDKVTEIPIKGEERVEEIIRGKEGEIVSPLFGRVVKIRVKEGDAVNKGQPLLSIEAMKAETVISSPIGGIVQKILIKEGQGVKKGDILIVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W00</accession>
    <name>Q97W00_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2450</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuvB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90416">
        <property type="entry name" value="G90416"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W00"/>
    <dbReference type="FunCoup" id="Q97W00">
        <property type="interactions" value="216"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2450"/>
    <dbReference type="PaxDb" id="273057-SSO2450"/>
    <dbReference type="EnsemblBacteria" id="AAK42590">
        <property type="protein sequence ID" value="AAK42590"/>
        <property type="gene ID" value="SSO2450"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2450"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2528"/>
    <dbReference type="eggNOG" id="arCOG04081">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028311_1_1_2"/>
    <dbReference type="InParanoid" id="Q97W00"/>
    <dbReference type="PhylomeDB" id="Q97W00"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097255">
        <property type="term" value="C:R2TP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000010">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000001">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="86"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="476" mass="53202" checksum="A3F0372EB340E1A6" modified="2001-10-01" version="1">MCHNIKPHRFRVNLKNFIIYYYCEMSEIRELKKPIREKASIHSHIKGLGLDSNGKAKFIADGLVGQAEAREAAGVVVQLIKQGKMSGKGILFVGPPGTGKTALAVAIARELGEDTPFTAINASEIYSTELKKTEILTQLIRKSIGVRIREKRLVYEGVVKDRKIKVARSRLNPYSQAPVEAQITLTTKDDERTLSVGEEIAQQLVSLGVKKGDVIMIDAQTGQVIVEGKAKGFEGAKTYDIETTKVLEMPTGPVRKEKEITTTLTLNDLDLNLAARNLAVTAIFSFFTEREINEDVRKEVDRLVKDWINQGRAELVVGVLFIDDAHTLDLEAFSFLTRALESELAPILILATNRGLTKIRGTDIESPHGIPLDLLDRLLIIPTRPYNADEIKEIIKIRADELEIELDPQALEELTKIGVENSLRYSVQLLEPSLIIAQRNNRSIIKVEDVVIASKLFSDVKRSVKFVKEYENLLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W09</accession>
    <name>Q97W09_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Amidohydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2438</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90415">
        <property type="entry name" value="F90415"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993123.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W09"/>
    <dbReference type="SMR" id="Q97W09"/>
    <dbReference type="STRING" id="273057.SSO2438"/>
    <dbReference type="PaxDb" id="273057-SSO2438"/>
    <dbReference type="DNASU" id="1453908"/>
    <dbReference type="EnsemblBacteria" id="AAK42581">
        <property type="protein sequence ID" value="AAK42581"/>
        <property type="gene ID" value="SSO2438"/>
    </dbReference>
    <dbReference type="GeneID" id="1453908"/>
    <dbReference type="KEGG" id="sso:SSO2438"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2519"/>
    <dbReference type="eggNOG" id="arCOG00695">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_741058_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W09"/>
    <dbReference type="PhylomeDB" id="Q97W09"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050287">
        <property type="entry name" value="MTA/SAH_deaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794:SF11">
        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794">
        <property type="entry name" value="AMINOHYDROLASE SSNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="43139" checksum="C9EA575E55582A72" modified="2001-10-01" version="1">MKVLIKTDLVLGAPKTLRDVYIGIDEGQIKVISKEKPEDYDYAEYVIGGKNRVVAPGFVTTHSFLYLYPFRYRVFSGKANAFQLMSTLTPNDIYYFSLMGAYHLLKTGVTTVVTSGPNLDMIARAISEVGLRPVLAVGVDCPDSKEDWEREFTTLYNRWSSKNENRVILRLCSNEYSKEVFELSQQYDFPVLLERFVSLENISGNPINVIGLGGGARKDLDIIKQKGFHLASTPSYEVSQFPLSQYKPSISLDLSPTFDIRHEVSTSITRLILTPEEALNAMTIWGLLQLKYNDRGVLENGKVADLVIFEVKEPPTFPLDYESPYESIVFNLSTPETVLVSGEAILDGGVPLNIGIKHIEKAIERLEDIDKKVTRPARYMEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WF0</accession>
    <name>Q97WF0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-oxoacyl-(Acyl carrier protein) reductase (FabG-5)</fullName>
            <ecNumber evidence="3">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fabG-5</name>
        <name evidence="3" type="ordered locus">SSO2276</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90397">
        <property type="entry name" value="G90397"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991843.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WF0"/>
    <dbReference type="FunCoup" id="Q97WF0">
        <property type="interactions" value="162"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2276"/>
    <dbReference type="PaxDb" id="273057-SSO2276"/>
    <dbReference type="EnsemblBacteria" id="AAK42438">
        <property type="protein sequence ID" value="AAK42438"/>
        <property type="gene ID" value="SSO2276"/>
    </dbReference>
    <dbReference type="GeneID" id="1453765"/>
    <dbReference type="KEGG" id="sso:SSO2276"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2368"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_2"/>
    <dbReference type="InParanoid" id="Q97WF0"/>
    <dbReference type="PhylomeDB" id="Q97WF0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05344">
        <property type="entry name" value="BKR_like_SDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43639">
        <property type="entry name" value="OXIDOREDUCTASE, SHORT-CHAIN DEHYDROGENASE/REDUCTASE FAMILY (AFU_ORTHOLOGUE AFUA_5G02870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43639:SF1">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="261" mass="28060" checksum="18376FD13364D064" modified="2001-10-01" version="1">MDLGIKGKKVIVTAASKGIGLAIAKRFLEEGAKVIISSNDESNLMKAYNKLKEIHSENVSYIKADLMNPHEVKSLVHEGYRKLGGLDVLAYVTGSPKPGNLFSLSDKDWMDAFNLLLLSAVVAVREAGQLMQSGGRIILSTSTTLRQPIDNLDLSNVVRLSLAGLIKVASRELGPKGILVNGVMPGWTKTERLLQIIRDRAIREGKSEDEVLKSIVADVPLGRAGEPEEIANVVLFLASNLSTYVTGALIPVDGGSIKGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WG6</accession>
    <name>Q97WG6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Bifunctional NAD(P)H-hydrate repair enzyme</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Nicotinamide nucleotide repair protein</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="19">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
                <ecNumber evidence="19">4.2.1.136</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="19">ADP-dependent NAD(P)HX dehydratase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="19">NAD(P)H-hydrate epimerase</fullName>
                <ecNumber evidence="19">5.1.99.6</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="17" type="primary">nnrD</name>
        <name evidence="18" type="synonym">nnrE</name>
        <name evidence="22" type="ordered locus">SSO2254</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 19">Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18 19">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 18 19">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17 19">
            <text>(6S)-NADHX + ADP = AMP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17 19">
            <text>(6S)-NADPHX + ADP = AMP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 19">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="18 19">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">In the C-terminal section; belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">In the N-terminal section; belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="19"/>
    <dbReference type="EC" id="5.1.99.6" evidence="19"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90395">
        <property type="entry name" value="F90395"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991536.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WG6"/>
    <dbReference type="FunCoup" id="Q97WG6">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2254"/>
    <dbReference type="PaxDb" id="273057-SSO2254"/>
    <dbReference type="EnsemblBacteria" id="AAK42421">
        <property type="protein sequence ID" value="AAK42421"/>
        <property type="gene ID" value="SSO2254"/>
    </dbReference>
    <dbReference type="GeneID" id="1453748"/>
    <dbReference type="KEGG" id="sso:SSO2254"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2348"/>
    <dbReference type="eggNOG" id="arCOG00018">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024853_4_1_2"/>
    <dbReference type="InParanoid" id="Q97WG6"/>
    <dbReference type="PhylomeDB" id="Q97WG6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NAD(P)HX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000119">
        <property type="entry name" value="Multifunctional fusion protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030677">
        <property type="entry name" value="Nnr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00197">
        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017184">
        <property type="entry name" value="Nnr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 18 19" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12 17 19" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 18 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 17 19" id="KW-0520">NAD</keyword>
    <keyword evidence="8 17 19" id="KW-0521">NADP</keyword>
    <keyword evidence="6 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 18 19" id="KW-0630">Potassium</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF N-terminal" evidence="21">
        <location>
            <begin position="7"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF C-terminal" evidence="20">
        <location>
            <begin position="211"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="126"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR017184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="AAK42421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="501" mass="54309" checksum="B67C0F1CD6F400EC" modified="2001-10-01" version="1">MISVKEMRALEINSSALGVSTLLLMENAGRSVKDEIVKRFNVKDKVAYVYVGHGGKGGDGLVAARHLADEGAKVTVILLGENKHEDAILNLNVIEEMDYSITLVEIKDMDELKPISADILIDAMLGTGFSGKPREPFRSAIKAFNNSKGFKVSIDVPSGINADTGEAYEDEYVKPDLVVTFHDIKPGLLKYNFNTVVTKIGIPVEAEIYVGPGDLIVNARSRPYYSKKGDSGRVLVIGGSYTFSGAPTLAALGALRAGADLVYVASPEDTARIIAGYSPDLITIKLRGKNISPDNFEELKLWIDRADVVVIGPGMGLAEETIEASKLIVNYLKEKNKLAVIDADALKAISGFDLYENAVITPHAGEFKIFFGEEPDKNIRDRISQVITYAKKCKCTVLLKGYVDIISDGKRFKLNKTGNPGMTVGGSGDTLTGITATLMAQKIEPFIAAYLGVFINSLAGTLAYNRLGAHLTPTDIINEIPNVINNPLDSFKRKLYKRVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WJ9</accession>
    <name>Q97WJ9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2218</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90391">
        <property type="entry name" value="D90391"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992064.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WJ9"/>
    <dbReference type="STRING" id="273057.SSO2218"/>
    <dbReference type="PaxDb" id="273057-SSO2218"/>
    <dbReference type="EnsemblBacteria" id="AAK42387">
        <property type="protein sequence ID" value="AAK42387"/>
        <property type="gene ID" value="SSO2218"/>
    </dbReference>
    <dbReference type="GeneID" id="1453714"/>
    <dbReference type="KEGG" id="sso:SSO2218"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2313"/>
    <dbReference type="eggNOG" id="arCOG00721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109674_2_0_2"/>
    <dbReference type="InParanoid" id="Q97WJ9"/>
    <dbReference type="PhylomeDB" id="Q97WJ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.30">
        <property type="entry name" value="RNA polymerase ii, chain L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="4">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="158" mass="17994" checksum="0E8026DE949BE869" modified="2001-10-01" version="1">MHIIFDTSGFLSGLQLSLDRVYTTQEVINEIKDKYSRFNLEIAISSGKVIIMKPSTRSVEKVTKVLNLTKERKLSNTDISVIALALDLQPSIVFTDDLSVQNILKQLGIQFSSVKINKKVEKSFKFKYVCVNCKREFNIDHGECPYCGGKVVKRRIME</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WP2</accession>
    <name>Q97WP2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chlorite dismutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2169</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99386">
        <property type="entry name" value="A99386"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992137.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WP2"/>
    <dbReference type="STRING" id="273057.SSO2169"/>
    <dbReference type="PaxDb" id="273057-SSO2169"/>
    <dbReference type="EnsemblBacteria" id="AAK42344">
        <property type="protein sequence ID" value="AAK42344"/>
        <property type="gene ID" value="SSO2169"/>
    </dbReference>
    <dbReference type="GeneID" id="15299165"/>
    <dbReference type="KEGG" id="sso:SSO2169"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2264"/>
    <dbReference type="eggNOG" id="arCOG03031">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076582_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WP2"/>
    <dbReference type="PhylomeDB" id="Q97WP2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1030">
        <property type="entry name" value="Apc35880, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010644">
        <property type="entry name" value="ChdC/CLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36843:SF1">
        <property type="entry name" value="COPROHEME DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36843">
        <property type="entry name" value="HEME-DEPENDENT PEROXIDASE YWFI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06778">
        <property type="entry name" value="Chlor_dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="26537" checksum="F80477B78D133C76" modified="2001-10-01" version="1">MSQNSLAYFYISSIKLTNRWWSSSREERRSAINEIESVESQFRNNLISLKRYISLRNDSDIIYWVTSSDTSKLLEFKYTLLSKIRDLGYESLSLFSVYRSSPYTRGNFDINKVLSLEPLRYFVAYPMKKDVEWYLLPFEEREKIMKEHIETARTHPKNKGIRSYTTYSFGVGDYEFVVVYEIPEIENWVEVVEALREVKARKWITKEEPILVGELRGLDLFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WS9</accession>
    <name>Q97WS9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thermopsin, probable</fullName>
            <ecNumber evidence="2">3.4.23.42</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.23.42" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90370">
        <property type="entry name" value="H90370"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WS9"/>
    <dbReference type="STRING" id="273057.SSO2037"/>
    <dbReference type="PaxDb" id="273057-SSO2037"/>
    <dbReference type="EnsemblBacteria" id="AAK42223">
        <property type="protein sequence ID" value="AAK42223"/>
        <property type="gene ID" value="SSO2037"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2037"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2118"/>
    <dbReference type="eggNOG" id="arCOG03670">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_494031_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WS9"/>
    <dbReference type="PhylomeDB" id="Q97WS9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007981">
        <property type="entry name" value="Peptidase_A5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05317">
        <property type="entry name" value="Thermopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="548"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="570" mass="63796" checksum="3B880B44F0200727" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WU1</accession>
    <name>Q97WU1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamine amidotransferase type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90369">
        <property type="entry name" value="B90369"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923731.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WU1"/>
    <dbReference type="STRING" id="273057.SSO2023"/>
    <dbReference type="PaxDb" id="273057-SSO2023"/>
    <dbReference type="EnsemblBacteria" id="AAK42209">
        <property type="protein sequence ID" value="AAK42209"/>
        <property type="gene ID" value="SSO2023"/>
    </dbReference>
    <dbReference type="GeneID" id="7811499"/>
    <dbReference type="KEGG" id="sso:SSO2023"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2099"/>
    <dbReference type="eggNOG" id="arCOG03639">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086262_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WU1"/>
    <dbReference type="PhylomeDB" id="Q97WU1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42824">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42824:SF1">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YAFJ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="1">
        <location>
            <begin position="2"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="228" mass="26272" checksum="A46634CB0DFE0931" modified="2001-10-01" version="1">MCRMLAYHGDDKEYLKRLTNCLAKASLNDPLTNETHGDGWGIVAIASNNLIHYRSHLPIFKDENLEKILNLLDGEMKVIIHARQASDKRLISPYYSHPYLESTPKSILFLAHNGSVDKYKLGNVLGIDPMLMVDSELVAKYFATYSIKEIDKLQDVTQSALNLLIMEINRIDRSSTIYFYNYYRKDRIKRKEEYYKLYFNKGAIYSSSLAYTGCEKGEEVKFGNLGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WZ4</accession>
    <name>Q97WZ4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1913</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO9368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90362">
        <property type="entry name" value="F90362"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WZ4"/>
    <dbReference type="STRING" id="273057.SSO9368"/>
    <dbReference type="PaxDb" id="273057-SSO9368"/>
    <dbReference type="EnsemblBacteria" id="AAK42157">
        <property type="protein sequence ID" value="AAK42157"/>
        <property type="gene ID" value="SSO9368"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO9368"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2040"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_4_1_2"/>
    <dbReference type="InParanoid" id="Q97WZ4"/>
    <dbReference type="PhylomeDB" id="Q97WZ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="4"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="98" mass="11174" checksum="D870AF6EC4CDCCA2" modified="2001-10-01" version="1">MFYIRSVDIVLITYKDRLTRFGFEYIEEFFSTMGVKIEVVFGEEPKDDAQELVEDLISIITSFAGKIYGMRSHKKTLLVQGVKKLIGELSGEDSEVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X08</accession>
    <name>Q97X08_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Endoglucanase</fullName>
            <ecNumber evidence="3">3.2.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1949</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 12 (cellulase H) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90360">
        <property type="entry name" value="G90360"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989083.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X08"/>
    <dbReference type="SMR" id="Q97X08"/>
    <dbReference type="STRING" id="273057.SSO1949"/>
    <dbReference type="CAZy" id="GH12">
        <property type="family name" value="Glycoside Hydrolase Family 12"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1949"/>
    <dbReference type="EnsemblBacteria" id="AAK42142">
        <property type="protein sequence ID" value="AAK42142"/>
        <property type="gene ID" value="SSO1949"/>
    </dbReference>
    <dbReference type="GeneID" id="7798846"/>
    <dbReference type="KEGG" id="sso:SSO1949"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2024"/>
    <dbReference type="eggNOG" id="arCOG03858">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_768611_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X08"/>
    <dbReference type="PhylomeDB" id="Q97X08"/>
    <dbReference type="BRENDA" id="3.2.1.4">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013319">
        <property type="entry name" value="GH11/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002594">
        <property type="entry name" value="GH12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01670">
        <property type="entry name" value="Glyco_hydro_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="334" mass="37619" checksum="CB3F613936393A48" modified="2001-10-01" version="1">MIMNKLYIIIVPIIVIIVVGVIGGAIYLHHQSPNVKTSSITVTTNETTTLMSITTNTVPTTVTPTTSSIPQLIYVTSSASSPTPVYLNNSTVPSFYLEVNMWNAKTWNGNYTMVFNPLTRTLSVSFNLTQVNPLQWTNGYPEIYVGRKPWDTSYAGNIFPMRIGNMTPFMVSFYINLTKLDPSINFDIASDAWIVRPQIAFSPGTAPGNGDIEIMVWLFSQNLQPAGQQVGEVVIPIYINHTLVNATFQVWKMKNVPWGGWEYIAFRPDGWKVTNGYVAYEPNLFIKALNNFASYNITNYYLTDWEFGTEWGTMTSNGTAYFSWTISNFYETLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X28</accession>
    <name>Q97X28_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO9268</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99358">
        <property type="entry name" value="A99358"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X28"/>
    <dbReference type="STRING" id="273057.SSO9268"/>
    <dbReference type="PaxDb" id="273057-SSO9268"/>
    <dbReference type="EnsemblBacteria" id="AAK42120">
        <property type="protein sequence ID" value="AAK42120"/>
        <property type="gene ID" value="SSO9268"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO9268"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2000"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2434011_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X28"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="90" mass="10627" checksum="B32460396FD7A72C" modified="2001-10-01" version="1">MERTVKLRVKVDNKTYQKLKEVEEEYKKILEDTINYGLVNKTTSFTRIKSGVYKTEREKHKDLPSHYIYTACEDASERRSVINLSVKLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X39</accession>
    <name>Q97X39_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Second ORF in transposon ISC1913</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1916</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90356">
        <property type="entry name" value="F90356"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923650.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X39"/>
    <dbReference type="STRING" id="273057.SSO1916"/>
    <dbReference type="PaxDb" id="273057-SSO1916"/>
    <dbReference type="EnsemblBacteria" id="AAK42109">
        <property type="protein sequence ID" value="AAK42109"/>
        <property type="gene ID" value="SSO1916"/>
    </dbReference>
    <dbReference type="GeneID" id="1453436"/>
    <dbReference type="KEGG" id="sso:SSO1916"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1980"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_17_0_2"/>
    <dbReference type="InParanoid" id="Q97X39"/>
    <dbReference type="PhylomeDB" id="Q97X39"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="295"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="408" mass="47734" checksum="DD4F88DB6ED2E25D" modified="2001-10-01" version="1">MERTVKLRVKVDYKTYSALKEVEKEYREVLEEAINYGLSNKTTSFTRIKAGVYKTEREKHKDLPSHYIYTACEDASERLDSFEKLKRRGRSYTEKPSVRRVTIHLDDHLWKFNLDRISISTKRSRILISPTFPKIFWRYYNKGWRIASEARFKLLKGNVVEFYVIFKRDEPKPYEPKGFISVDLNENSVSVLVDGKPMLLETNTKRITLGYEYRRKAITTGKSTKDREVRRKLKRLRERNKKVDIRRKLAKLIVKEAFESRSVIVLEDLPRRTPEHMIKDVKDSQLRLRIYRAAFSSMKNAIIEKAREFGVPVVLVNPSHTSTVCPVHGTKIVYQLDGGDAPRVGVCEKGKEKWHRDVVALYNLARRAGDVSPVPLGSKESHDPPKRWLRAKSLHSIMNDHKMIEMKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X41</accession>
    <name>Q97X41_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1914</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90356">
        <property type="entry name" value="D90356"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992973.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X41"/>
    <dbReference type="PaxDb" id="273057-SSO1914"/>
    <dbReference type="EnsemblBacteria" id="AAK42107">
        <property type="protein sequence ID" value="AAK42107"/>
        <property type="gene ID" value="SSO1914"/>
    </dbReference>
    <dbReference type="GeneID" id="1453434"/>
    <dbReference type="KEGG" id="sso:SSO1914"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1976"/>
    <dbReference type="eggNOG" id="arCOG00727">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121774_1_4_2"/>
    <dbReference type="InParanoid" id="Q97X41"/>
    <dbReference type="PhylomeDB" id="Q97X41"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09873">
        <property type="entry name" value="PIN_Pae0151-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044153">
        <property type="entry name" value="PIN_Pae0151-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051619">
        <property type="entry name" value="TypeII_TA_RNase_PINc/VapC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901:SF1">
        <property type="entry name" value="EXONUCLEASE VAPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901">
        <property type="entry name" value="RIBONUCLEASE VAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="2">
        <location>
            <begin position="3"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="137" mass="16092" checksum="5C28BA615B47A9B3" modified="2001-10-01" version="1">MAIIDTSALFAIYFPEKMTDFIRREIERVEECYFLDLIFYEFPNVIRKRIVRNELSREKADEILLRALSYIDLCKIVSGKELTWTAYEISLKYSLTTYDASLIALAKKVGDVILTADEKLLRGIRNFPEISKYFIFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X66</accession>
    <name>Q97X66_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90352">
        <property type="entry name" value="F90352"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991000.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X66"/>
    <dbReference type="PaxDb" id="273057-SSO1885"/>
    <dbReference type="EnsemblBacteria" id="AAK42077">
        <property type="protein sequence ID" value="AAK42077"/>
        <property type="gene ID" value="SSO1885"/>
    </dbReference>
    <dbReference type="GeneID" id="1453404"/>
    <dbReference type="KEGG" id="sso:SSO1885"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1942"/>
    <dbReference type="eggNOG" id="arCOG07202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_965116_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X66"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="290" mass="33904" checksum="26495699C7154FF5" modified="2001-10-01" version="1">MNLNSHILLALAFGLILFHNDIALAVLVGIGAAIPDLDREYVFTKRKIFAKYQLHRALFHNIFFALAVTYFNLYLGLGIFLHIALDLLTSPTDRGVELFFPLGRLVKNFELDYDGNIRQSKGMMWYLEDPVRIINKTADPGLKVVVKMPWIRIYGPFKNSRLVDWMIFYSSFIFIQLYELNNLITWWETFLYTVFVKYVFIDIGIVLFYATGELWRRRLQFRNLNNKMKYVIIGVMTFGLSLIIFQGLYLYSPMKPIINLNTSLLIIVSMLIGLSLAYIHVRIRFKKITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X67</accession>
    <name>Q97X67_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90352">
        <property type="entry name" value="E90352"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991002.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X67"/>
    <dbReference type="PaxDb" id="273057-SSO1884"/>
    <dbReference type="EnsemblBacteria" id="AAK42076">
        <property type="protein sequence ID" value="AAK42076"/>
        <property type="gene ID" value="SSO1884"/>
    </dbReference>
    <dbReference type="GeneID" id="1453403"/>
    <dbReference type="KEGG" id="sso:SSO1884"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1941"/>
    <dbReference type="eggNOG" id="arCOG01205">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159724_1_0_2"/>
    <dbReference type="InParanoid" id="Q97X67"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37030">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37030:SF3">
        <property type="entry name" value="POLYMERASE NUCLEOTIDYL TRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="20"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="114" mass="13369" checksum="2669CB73E8DCB069" modified="2001-10-01" version="1">MSSWVKFRFSHLRRWREYAEKIAKATADLEPDSEVYVIGGVAEDRITVLSDIDILILIKRIIDSKEKKKLREVILLRAMDFYGLPFDAPVEIHIEDEEGAKRFFELSKKVIKIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X82</accession>
    <name>Q97X82_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfocyanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1872</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90350">
        <property type="entry name" value="E90350"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X82"/>
    <dbReference type="STRING" id="273057.SSO1872"/>
    <dbReference type="PaxDb" id="273057-SSO1872"/>
    <dbReference type="EnsemblBacteria" id="AAK42060">
        <property type="protein sequence ID" value="AAK42060"/>
        <property type="gene ID" value="SSO1872"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1872"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1923"/>
    <dbReference type="eggNOG" id="arCOG07181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136009_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X82"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="13307" checksum="7384B6620A30E536" modified="2001-10-01" version="1">MIQEMKKEPRAVSNAVFAGVVIVLVIIAAVGFALYATKPSTAPSTVTTPPSTTTLTYTQTSTQTMTSTQTATVTQTSTQTVTSTQVITRPVTVNASGAFYNGKVITFLYTSQFMCTPESVRNVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X99</accession>
    <name>Q97X99_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Second ORF in transposon ISC1359</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1853</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90348">
        <property type="entry name" value="D90348"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X99"/>
    <dbReference type="PaxDb" id="273057-SSO1853"/>
    <dbReference type="EnsemblBacteria" id="AAK42043">
        <property type="protein sequence ID" value="AAK42043"/>
        <property type="gene ID" value="SSO1853"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1853"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881170_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X99"/>
    <dbReference type="PhylomeDB" id="Q97X99"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="34"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="135" mass="16129" checksum="E5336936034D4056" modified="2001-10-01" version="1">MAELDISGFLCVRHGSGAYLIILRKEDDKIIAFLVRKEMDLYDAIVLAEMYRERWGIENAFRSLEEFRIRTRTCDVRKELVLVLLSYLLLNVWFLIRSWRKVKLWEFSVSLSNLLDREVRVEQGRAFREVKTPFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XG5</accession>
    <name>Q97XG5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISC1359</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90339">
        <property type="entry name" value="B90339"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XG5"/>
    <dbReference type="STRING" id="273057.SSO1775"/>
    <dbReference type="PaxDb" id="273057-SSO1775"/>
    <dbReference type="EnsemblBacteria" id="AAK41969">
        <property type="protein sequence ID" value="AAK41969"/>
        <property type="gene ID" value="SSO1775"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1775"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1826"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067987_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XG5"/>
    <dbReference type="PhylomeDB" id="Q97XG5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033541">
        <property type="entry name" value="transpos_ISH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="115"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="375" mass="43732" checksum="829BCC87C931D582" modified="2001-10-01" version="1">MKYRQNREDENFCRKVCIYEGELEHMKHENTTKPAKFNRDFARSALKIIYSILTKILFPEELLSALLKASGSYLSRLGKDGRRALRKLNAVQVEDVRDALKKMGRMTLRGVRNRRVAVDFHAIPQYHADKSFLSRIKPTKGTSWGLVQAAIFLLGRTRSFLDVIPVTVKNVAEGFKAVMEVIVKELEEDKLRLVMVFADREFAVNEVIRYLLELGLDFVISAKAQMYKKYKGMLQDVDVSFGGVRYTGFLCVRHGSGAYLIILRKEDGKIIAFLVRREIDLHDAIVLAEMYRERWGIENAFRSLEEFRIKTRTCDVRKELVLVLLSYLLLNVWFLIRSWRKVKLWEFSVSLSNLLDREVRVEQEHAFREVKTPFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XG7</accession>
    <name>Q97XG7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1774</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90338">
        <property type="entry name" value="H90338"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988984.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XG7"/>
    <dbReference type="PaxDb" id="273057-SSO1774"/>
    <dbReference type="EnsemblBacteria" id="AAK41967">
        <property type="protein sequence ID" value="AAK41967"/>
        <property type="gene ID" value="SSO1774"/>
    </dbReference>
    <dbReference type="GeneID" id="1454746"/>
    <dbReference type="KEGG" id="sso:SSO1774"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1824"/>
    <dbReference type="HOGENOM" id="CLU_992551_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XG7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017008">
        <property type="entry name" value="UCP032817-like"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032817">
        <property type="entry name" value="UCP032817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="280" mass="33388" checksum="646DABD79199C547" modified="2001-10-01" version="1">MSIINDEKLRKYVPEERLDKVKERLEILDTYDLSYITSNFIWKGRRFSPEQVWPIKIYYGKADRELAEKIEKEFRRFVALTIVFPKRLFTASGAVDLYWHFLILHTKEYEKFSKSVWGIPRYDNVLLKKLLNNDDNSFRLRFNLKPDNVNEITILPKKALESLDDKTLDRVLMLERLDLSPISENLLHTIDEFKFVPEQRFPLIAHFGKLDNNIVKRIELEFKKWMALNIAYGGKELNYAAPSGVVDMYFHLFILHTILYKQFCEIFFGKFIHHPLGELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XK1</accession>
    <name>Q97XK1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Partial ORF in transposase ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1732</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90334">
        <property type="entry name" value="D90334"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XK1"/>
    <dbReference type="PaxDb" id="273057-SSO1732"/>
    <dbReference type="EnsemblBacteria" id="AAK41931">
        <property type="protein sequence ID" value="AAK41931"/>
        <property type="gene ID" value="SSO1732"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1732"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1775"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113185_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XK1"/>
    <dbReference type="PhylomeDB" id="Q97XK1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="164" mass="18535" checksum="BA5AF82E744F2679" modified="2001-10-01" version="1">MEKAVNEVILSMTGTRKDVAKRLVLGAVVGRNATEIAQEAEMDYETVLNNLDKAAQAKLIEVVKKLVGDHPVLLIIDDTHDHKLYARAMPVSRNGAQIFYCRAHKRFEPAIQLLVIGVKDLVNNQIYVSILEKSVWESHFSCKNDNICKIEPHQFYSNITAYIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2025-06-18" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y16</accession>
    <name>Y1533_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Putative dioxygenase SSO1533</fullName>
            <ecNumber>1.13.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO1533</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the homogentisate dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.-.-"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90312">
        <property type="entry name" value="D90312"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923457.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y16"/>
    <dbReference type="SMR" id="Q97Y16"/>
    <dbReference type="FunCoup" id="Q97Y16">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1533"/>
    <dbReference type="PaxDb" id="273057-SSO1533"/>
    <dbReference type="DNASU" id="1454534"/>
    <dbReference type="EnsemblBacteria" id="AAK41755">
        <property type="protein sequence ID" value="AAK41755"/>
        <property type="gene ID" value="SSO1533"/>
    </dbReference>
    <dbReference type="GeneID" id="1454534"/>
    <dbReference type="KEGG" id="sso:SSO1533"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1572"/>
    <dbReference type="eggNOG" id="arCOG06976">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053101_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y16"/>
    <dbReference type="PhylomeDB" id="Q97Y16"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004411">
        <property type="term" value="F:homogentisate 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006570">
        <property type="term" value="P:tyrosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02208">
        <property type="entry name" value="cupin_RmlC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046451">
        <property type="entry name" value="HgmA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046452">
        <property type="entry name" value="HgmA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005708">
        <property type="entry name" value="Homogentis_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11056">
        <property type="entry name" value="HOMOGENTISATE 1,2-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11056:SF0">
        <property type="entry name" value="HOMOGENTISATE 1,2-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04209">
        <property type="entry name" value="HgmA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20510">
        <property type="entry name" value="HgmA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0223">Dioxygenase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000220260" description="Putative dioxygenase SSO1533">
        <location>
            <begin position="1"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="384" mass="44562" checksum="2656E16E1C36EE9F" modified="2001-10-01" version="1">MVFYHRQGELPKKRHTVFMKDGKLMREEVFGLNGFNGRYSLLYHLNPPTRIMKLGEWKSNTIGEWKDAEYRHHKLSTSKLRKSSDVFLDRIALIFNDNLIISFSKVDEGDTKLFYRNADFDEIYYVHSGEILFKSVFGEIEVKEGDYLVIPRGTTYTLSFKKNAELFIIEGKQIEVPKDYRNEYGQLVEGSFYYNRDLKLPVLRTYDENGNYKLVIKTVNGFQLVELDTHPFDVVGWDGYLYPFALSVYDLEPITGKLHQPPTAYTTFAADDFVVCTFVPRLFDYHPQSVPISYYHNNIDAEEIIFYSSGQFMSRRGINKGDITLHRGGHIHGPQPSAVEASLNKRGTYNDEVAIMVETQKRVKVSIYAKEIDDPTYPLSWYTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y51</accession>
    <name>Q97Y51_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99308">
        <property type="entry name" value="A99308"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923431.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y51"/>
    <dbReference type="PaxDb" id="273057-SSO1495"/>
    <dbReference type="EnsemblBacteria" id="AAK41720">
        <property type="protein sequence ID" value="AAK41720"/>
        <property type="gene ID" value="SSO1495"/>
    </dbReference>
    <dbReference type="GeneID" id="1454499"/>
    <dbReference type="KEGG" id="sso:SSO1495"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1535"/>
    <dbReference type="eggNOG" id="arCOG01149">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1207663_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y51"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="190" mass="21747" checksum="2ED05EF22D5C7A3E" modified="2001-10-01" version="1">MRFLLVSDIHKSFNFFRGHDESVAVDWLLEVISETEPEVLISAGDWDEGMTLEDFRRISSKVKLLTIYGNHENFPVIMNYAMPNSKVFDICGLKIAGINGLIGERSSKGVPLTKPTEFMNAIYKIKNSGIKVDILIAHQPPYIPEIYPKMKKDEYNELMFDAVEYLKPRLFFNGHMTAGCYSYYQFPSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YF2</accession>
    <name>Q97YF2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1373</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90293">
        <property type="entry name" value="H90293"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990015.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YF2"/>
    <dbReference type="STRING" id="273057.SSO1373"/>
    <dbReference type="PaxDb" id="273057-SSO1373"/>
    <dbReference type="EnsemblBacteria" id="AAK41607">
        <property type="protein sequence ID" value="AAK41607"/>
        <property type="gene ID" value="SSO1373"/>
    </dbReference>
    <dbReference type="GeneID" id="1454386"/>
    <dbReference type="KEGG" id="sso:SSO1373"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1378"/>
    <dbReference type="eggNOG" id="arCOG07239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1003328_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YF2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="272" mass="31476" checksum="CC90CE341E94D209" modified="2001-10-01" version="1">MGELSLASKKLIATLLKKVGIDNEDVNINEIMIRMNRTEIERILNGLSKLNIIGLKNGTITKGKVYSEGDKYLLFLNINKICSASPKGDRALRINFEATPLVNGVKERIHQSNRFNAEKASKVIRFNKITRKNLLDENKRGEGKWEKEVDPKLIKFRVTFDPPLEVGELIKYGFYTWDEKYFPSTMEELYRRYNMNYSMEGITIGQPTYYAKITINLPWVPKNVKAEKVLVVTLDGPHEGVEIQSEHRLAADRNKITLELWQPDYGTYFIKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YT1</accession>
    <name>Q97YT1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Toluene-4-monooxygenase system protein A. amino end (TmoA)</fullName>
            <ecNumber evidence="3">1.14.13.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tmoA</name>
        <name evidence="3" type="ordered locus">SSO1227</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.13.-" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90277">
        <property type="entry name" value="B90277"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923264.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YT1"/>
    <dbReference type="FunCoup" id="Q97YT1">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1227"/>
    <dbReference type="PaxDb" id="273057-SSO1227"/>
    <dbReference type="EnsemblBacteria" id="AAK41473">
        <property type="protein sequence ID" value="AAK41473"/>
        <property type="gene ID" value="SSO1227"/>
    </dbReference>
    <dbReference type="GeneID" id="1454252"/>
    <dbReference type="KEGG" id="sso:SSO1227"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1227"/>
    <dbReference type="eggNOG" id="arCOG08364">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1056091_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YT1"/>
    <dbReference type="PhylomeDB" id="Q97YT1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003430">
        <property type="entry name" value="Phenol_Hydrox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02332">
        <property type="entry name" value="Phenol_Hydrox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="268" mass="30705" checksum="DB1E6DCB5679B6F6" modified="2001-10-01" version="1">MKPVPVEPRLPSRDEYYDLANKLEWTPKYVKEEELFPVDMSGIPNLPIDVWTELYDAPYKVTYREYVKNQKEKDQRLFSVREAGVRAELVKKLDKTYHQGSFCFHITAVPIPEYTAAIAEARMARFGKAGEWRNLSTFGSMDEVRHAQLQILLSHDNIQASPKFAFAHKMFWVDGWLPIAARSFFDDTITGTNAIEFALLTTFAFETGFTNLQFVAYAAMANKTNDFVFSSATQSIQTDEARHAQIGHPVLKAFVDVTKVNEEMEKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="150" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YY4</accession>
    <name>Q97YY4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sugar ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1168</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99270">
        <property type="entry name" value="A99270"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991575.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YY4"/>
    <dbReference type="FunCoup" id="Q97YY4">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1168"/>
    <dbReference type="PaxDb" id="273057-SSO1168"/>
    <dbReference type="EnsemblBacteria" id="AAK41416">
        <property type="protein sequence ID" value="AAK41416"/>
        <property type="gene ID" value="SSO1168"/>
    </dbReference>
    <dbReference type="GeneID" id="1454195"/>
    <dbReference type="KEGG" id="sso:SSO1168"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1160"/>
    <dbReference type="eggNOG" id="arCOG00175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_2"/>
    <dbReference type="InParanoid" id="Q97YY4"/>
    <dbReference type="PhylomeDB" id="Q97YY4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055052">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000042">
        <property type="entry name" value="Maltose/maltodextrin ABC transporter, ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047641">
        <property type="entry name" value="ABC_transpr_MalK/UgpC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040582">
        <property type="entry name" value="OB_MalK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875">
        <property type="entry name" value="MALTODEXTRIN IMPORT ATP-BINDING PROTEIN MSMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875:SF15">
        <property type="entry name" value="TREHALOSE IMPORT ATP-BINDING PROTEIN SUGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17912">
        <property type="entry name" value="OB_MalK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="MOP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="5"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="368" mass="41885" checksum="6B728206043290F5" modified="2001-10-01" version="1">MMSYVKLENIWKEYEEKKRKVQVLRGLNLEIEKGEFVVILGPSGEGKTTILKIIAGLLRQDKGHVYLRGEVVDDLPPKDRRVAMVPQNYAIYPFMSVYDNIAFPLKVMHLSKDEIKKRVLEVARMLRIDNLLERKPSQLSGGQMQRVAIARALVKGADIILMDEPLSNLDAQVRVVAREELKQLQRDLKPTIIYVTHDQVEALSLATKVAILHDGVVQAYGDPMEIYKRPNSAWVGSFLGNPPMNIIRGEIEGDSIIIDSYRIGLPEEYKNLVKNGQKVLVGVRPEDMEIRNSGEIEGKVEMVEDLGPYSIIHLKINEETTIRVIEKSLSRRERGEKVRISIDTSKVVLFDASSERNLLEQYEKVSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-10-02" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z21</accession>
    <name>Q97Z21_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutaredoxin related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1120</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2014" name="Extremophiles" volume="18" first="219" last="228">
            <title>Sulfolobus solfataricus thiol redox puzzle: characterization of an atypical protein disulfide oxidoreductase.</title>
            <authorList>
                <person name="Limauro D."/>
                <person name="De Simone G."/>
                <person name="Pirone L."/>
                <person name="Bartolucci S."/>
                <person name="D'Ambrosio K."/>
                <person name="Pedone E."/>
            </authorList>
            <dbReference type="PubMed" id="24306780"/>
            <dbReference type="DOI" id="10.1007/s00792-013-0607-8"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS)</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99265">
        <property type="entry name" value="A99265"/>
    </dbReference>
    <dbReference type="PDB" id="4MNN">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A=1-171"/>
    </dbReference>
    <dbReference type="PDBsum" id="4MNN"/>
    <dbReference type="AlphaFoldDB" id="Q97Z21"/>
    <dbReference type="SMR" id="Q97Z21"/>
    <dbReference type="STRING" id="273057.SSO1120"/>
    <dbReference type="PaxDb" id="273057-SSO1120"/>
    <dbReference type="EnsemblBacteria" id="AAK41376">
        <property type="protein sequence ID" value="AAK41376"/>
        <property type="gene ID" value="SSO1120"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1120"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1116"/>
    <dbReference type="eggNOG" id="arCOG01218">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507297_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z21"/>
    <dbReference type="PhylomeDB" id="Q97Z21"/>
    <dbReference type="BRENDA" id="1.8.99.B1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97Z21"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02973">
        <property type="entry name" value="TRX_GRX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054067">
        <property type="entry name" value="SSO1120-like_N"/>
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        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37170:SF1">
        <property type="entry name" value="GLUTAREDOXIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37170">
        <property type="entry name" value="GLUTAREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21873">
        <property type="entry name" value="Thioredoxin_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="6" id="KW-0002">3D-structure</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SSO1120-like N-terminal thioredoxin-like" evidence="3">
        <location>
            <begin position="3"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin-like fold" evidence="2">
        <location>
            <begin position="103"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="111"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="4MNN"/>
        </source>
    </evidence>
    <sequence length="171" mass="19931" checksum="2DEBEE9468A639CA" modified="2001-10-01" version="1">MSYDYVIKEYLNAIKKGDITIQQCNGDSLFHEFKNYVNVETLNNCKKPLVKVKRGDRVYYTYYGIPIANELWPFLNSLVRISNNVVNLDEREVELAKQVRGSVKLFVTPDCTKCPITAEFLYQVSQINENVKLEIYDATEYEEERDKYRVLSVPKIIFNDKVEIPGDSLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z57</accession>
    <name>Q97Z57_SACS2</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1076</name>
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    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90260">
        <property type="entry name" value="C90260"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z57"/>
    <dbReference type="SMR" id="Q97Z57"/>
    <dbReference type="STRING" id="273057.SSO1076"/>
    <dbReference type="PaxDb" id="273057-SSO1076"/>
    <dbReference type="DNASU" id="1454117"/>
    <dbReference type="EnsemblBacteria" id="AAK41338">
        <property type="protein sequence ID" value="AAK41338"/>
        <property type="gene ID" value="SSO1076"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1076"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1076"/>
    <dbReference type="eggNOG" id="arCOG05973">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369558_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z57"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="190" mass="21430" checksum="577A25BA865AE8B9" modified="2001-10-01" version="1">MKVGYLIGSNGYITNLNNAIKLEVIDGSKEIISLAYGKISDIPKLDVLIGRDFLQGEVLFLKNYANFLIDKNRLTNIIPIQLPLHNQDAICVGTSNCSGISFKDVIFTYPFPFRDSSFNSAIIFEVLDLDIIREVYRVVKSGSKVYMILRDKLFGGVDPLEGLRKLSSKFKVAMVKEKEGFWIIEGVKKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2026-01-28" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z82</accession>
    <name>GIN15_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA replication complex GINS protein Gins15</fullName>
            <shortName evidence="3">Gins15</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gins15</name>
        <name evidence="5" type="ordered locus">SSO1049</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="EMBO Rep." volume="7" first="539" last="545">
            <title>GINS, a central nexus in the archaeal DNA replication fork.</title>
            <authorList>
                <person name="Marinsek N."/>
                <person name="Barry E.R."/>
                <person name="Makarova K.S."/>
                <person name="Dionne I."/>
                <person name="Koonin E.V."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="16485022"/>
            <dbReference type="DOI" id="10.1038/sj.embor.7400649"/>
        </citation>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="journal article" date="2025" name="Acta Crystallogr. F Struct. Biol. Commun." volume="81" first="207" last="215">
            <title>Structure of the Saccharolobus solfataricus GINS tetramer.</title>
            <authorList>
                <person name="Shankar S."/>
                <person name="Enemark E.J."/>
            </authorList>
            <dbReference type="PubMed" id="40235367"/>
            <dbReference type="DOI" id="10.1107/s2053230x25003085"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The CG complex (Cdc45, Gins15 and Gins23) stimulates the helicase activity of the MCM replicative DNA helicase; it copurifies throughout the cell cycle with MCM (as presumably does the CG complex) probably associated with moving replication forks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the Cdc45-GINS complex (CG complex) that includes Cdc45, Gins15 and Gins23 (PubMed:16485022). The GINS complex has 2:2 stoichiometry (PubMed:16485022, PubMed:40235367). Directly interacts with itself, Cdc45 and Gins23 (PubMed:16485022).</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-8081384">
            <id>Q97Z82</id>
        </interactant>
        <interactant intactId="EBI-8081609">
            <id>Q980J6</id>
            <label>SSO0295</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>3</experiments>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-8081384">
            <id>Q97Z82</id>
        </interactant>
        <interactant intactId="EBI-8081318">
            <id>Q9UXG0</id>
            <label>ORF-c40_009</label>
        </interactant>
        <organismsDiffer>true</organismsDiffer>
        <experiments>5</experiments>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="H90256"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989179.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9MOJ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B=1-151"/>
    </dbReference>
    <dbReference type="PDBsum" id="9MOJ"/>
    <dbReference type="AlphaFoldDB" id="Q97Z82"/>
    <dbReference type="SMR" id="Q97Z82"/>
    <dbReference type="IntAct" id="Q97Z82">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="MINT" id="Q97Z82"/>
    <dbReference type="STRING" id="273057.SSO1049"/>
    <dbReference type="PaxDb" id="273057-SSO1049"/>
    <dbReference type="EnsemblBacteria" id="AAK41311">
        <property type="protein sequence ID" value="AAK41311"/>
        <property type="gene ID" value="SSO1049"/>
    </dbReference>
    <dbReference type="GeneID" id="1454090"/>
    <dbReference type="KEGG" id="sso:SSO1049"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1043"/>
    <dbReference type="eggNOG" id="arCOG00551">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1709217_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z82"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059062">
        <property type="entry name" value="Gins15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059061">
        <property type="entry name" value="Gins15_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25865">
        <property type="entry name" value="Gins15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25864">
        <property type="entry name" value="Gins15_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000463481" description="DNA replication complex GINS protein Gins15">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q4J9A9"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16485022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="16485022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9MOJ"/>
        </source>
    </evidence>
    <sequence length="151" mass="17814" checksum="857E458321AEE4F8" modified="2001-10-01" version="1">MLDELVKKELSEEEITEIKLEEIIKYITLIKKSKTFVSSEIRKEELKFLSELAESLFELRLSKVLEGKVGKGFDEFIFDIFKILKQFYVDLLTGRYIIYNDKIYCIVQKPLIYNDHRVNEGDVLVLPMREALPLIIASYLTPYKIDIEEQL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-05" modified="2026-01-28" version="139" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z84</accession>
    <name>PCNA2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA polymerase sliding clamp 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Proliferating cell nuclear antigen homolog 2</fullName>
            <shortName evidence="1">PCNA2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pcn2</name>
        <name type="synonym">pcnA-2</name>
        <name type="ordered locus">SSO1047</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Mol. Cell" volume="11" first="275" last="282">
            <title>A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Dionne I."/>
                <person name="Nookala R.K."/>
                <person name="Jackson S.P."/>
                <person name="Doherty A.J."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="12535540"/>
            <dbReference type="DOI" id="10.1016/S1097-2765(02)00824-9"/>
        </citation>
        <scope>INTERACTION WITH DPO1 AND PCNA1</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Mol. Microbiol." volume="48" first="361" last="371">
            <title>An archaeal XPF repair endonuclease dependent on a heterotrimeric PCNA.</title>
            <authorList>
                <person name="Roberts J.A."/>
                <person name="Bell S.D."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="12675797"/>
            <dbReference type="DOI" id="10.1046/j.1365-2958.2003.03444.x"/>
        </citation>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2006" name="J. Mol. Biol." volume="364" first="243" last="247">
            <title>PCNA activates the Holliday junction endonuclease Hjc.</title>
            <authorList>
                <person name="Dorazi R."/>
                <person name="Parker J.L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="17011573"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2006.09.011"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2006" name="Acta Crystallogr. F Struct. Biol. Commun." volume="62" first="944" last="948">
            <title>Structure of the heterotrimeric PCNA from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Williams G.J."/>
                <person name="Johnson K."/>
                <person name="Rudolf J."/>
                <person name="McMahon S.A."/>
                <person name="Carter L."/>
                <person name="Oke M."/>
                <person name="Liu H."/>
                <person name="Taylor G.L."/>
                <person name="White M.F."/>
                <person name="Naismith J.H."/>
            </authorList>
            <dbReference type="PubMed" id="17012780"/>
            <dbReference type="DOI" id="10.1107/S1744309106034075"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2006" name="Mol. Cell" volume="24" first="279" last="291">
            <title>A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA.</title>
            <authorList>
                <person name="Pascal J.M."/>
                <person name="Tsodikov O.V."/>
                <person name="Hura G.L."/>
                <person name="Song W."/>
                <person name="Cotner E.A."/>
                <person name="Classen S."/>
                <person name="Tomkinson A.E."/>
                <person name="Tainer J.A."/>
                <person name="Ellenberger T."/>
            </authorList>
            <dbReference type="PubMed" id="17052461"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2006.08.015"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.79 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2006" name="Nucleic Acids Res." volume="34" first="4515" last="4526">
            <title>Structure of an archaeal PCNA1-PCNA2-FEN1 complex: elucidating PCNA subunit and client enzyme specificity.</title>
            <authorList>
                <person name="Dore A.S."/>
                <person name="Kilkenny M.L."/>
                <person name="Jones S.A."/>
                <person name="Oliver A.W."/>
                <person name="Roe S.M."/>
                <person name="Bell S.D."/>
                <person name="Pearl L.H."/>
            </authorList>
            <dbReference type="PubMed" id="16945955"/>
            <dbReference type="DOI" id="10.1093/nar/gkl623"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) IN COMPLEX WITH PCNA1 AND FEN</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF 146-GLU--ASP-149</scope>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2008" name="Acta Crystallogr. D" volume="64" first="941" last="949">
            <title>Structures of monomeric, dimeric and trimeric PCNA: PCNA-ring assembly and opening.</title>
            <authorList>
                <person name="Hlinkova V."/>
                <person name="Xing G."/>
                <person name="Bauer J."/>
                <person name="Shin Y.J."/>
                <person name="Dionne I."/>
                <person name="Rajashankar K.R."/>
                <person name="Bell S.D."/>
                <person name="Ling H."/>
            </authorList>
            <dbReference type="PubMed" id="18703842"/>
            <dbReference type="DOI" id="10.1107/S0907444908021665"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="9">
        <citation type="journal article" date="2009" name="Mol. Microbiol." volume="71" first="678" last="691">
            <title>Structural insight into recruitment of translesion DNA polymerase Dpo4 to sliding clamp PCNA.</title>
            <authorList>
                <person name="Xing G."/>
                <person name="Kirouac K."/>
                <person name="Shin Y.J."/>
                <person name="Bell S.D."/>
                <person name="Ling H."/>
            </authorList>
            <dbReference type="PubMed" id="19054331"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.2008.06553.x"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 1-244 IN COMPLEX WITH DPO4</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="5">One of the sliding clamp subunits that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication. Heterotrimer stimulates the Holliday junction resolvase Hjc. DNA polymerase I, DNA ligase and the flap endonuclease may be constitutively associated with the PCNA heterotrimer forming a scanning complex able to couple DNA synthesis and Okazaki fragment maturation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3 4 5 6 7 8 9">Forms homodimers with PCNA1, which then recruit PCNA3; does not form homotrimers (PubMed:12535540, PubMed:18703842). The heterodimers interact with RfcS homotetramers (PubMed:12535540). Heterotrimer which circularizes head-to-tail (head is at N-terminus, tail is at C-terminus) to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA. This subunit interacts with DNA polymerase I (dpo1) (PubMed:12535540). The heterotrimer also interacts with flap endonuclease 1, DNA ligase and XPF via the other subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PCNA family.</text>
    </comment>
    <comment type="sequence caution" evidence="10">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK41309" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
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        <property type="protein sequence ID" value="AAK41309.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="PIR" id="F90256">
        <property type="entry name" value="F90256"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.79 A"/>
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    <dbReference type="PDB" id="2HIK">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.30 A"/>
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    <dbReference type="PDB" id="2IO4">
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        <property type="resolution" value="2.60 A"/>
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        <property type="resolution" value="2.20 A"/>
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        <property type="resolution" value="2.90 A"/>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
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        <property type="resolution" value="2.05 A"/>
        <property type="chains" value="D=1-244"/>
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        <property type="method" value="EM"/>
        <property type="resolution" value="4.16 A"/>
        <property type="chains" value="B=1-245"/>
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        <property type="method" value="EM"/>
        <property type="resolution" value="4.38 A"/>
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        <property type="method" value="EM"/>
        <property type="resolution" value="4.20 A"/>
        <property type="chains" value="B=1-245"/>
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    <dbReference type="AlphaFoldDB" id="Q97Z84"/>
    <dbReference type="EMDB" id="EMD-24618"/>
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    <dbReference type="STRING" id="273057.SSO1047"/>
    <dbReference type="PaxDb" id="273057-SSO1047"/>
    <dbReference type="EnsemblBacteria" id="AAK41309">
        <property type="protein sequence ID" value="AAK41309"/>
        <property type="gene ID" value="SSO1047"/>
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    <dbReference type="GeneID" id="1454088"/>
    <dbReference type="GeneID" id="44129976"/>
    <dbReference type="KEGG" id="sso:SSO1047"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1041"/>
    <dbReference type="eggNOG" id="arCOG00488">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_043978_1_1_2"/>
    <dbReference type="InParanoid" id="Q97Z84"/>
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    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA polymerase sliding clamp 2"/>
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        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA_clamp_sf"/>
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        <property type="entry name" value="Pr_cel_nuc_antig"/>
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        <property type="entry name" value="PRK01115.1-3"/>
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        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
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    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
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        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
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    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000149216" description="DNA polymerase sliding clamp 2">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of interaction with PCNA3, no change with PCNA1." evidence="4">
        <original>LSDL</original>
        <variation>KLSK</variation>
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="1"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="helix" evidence="12">
        <location>
            <begin position="8"/>
            <end position="19"/>
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    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="23"/>
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    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="33"/>
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    <feature type="strand" evidence="12">
        <location>
            <begin position="43"/>
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    <feature type="helix" evidence="12">
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            <begin position="53"/>
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    <feature type="strand" evidence="12">
        <location>
            <begin position="56"/>
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    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="66"/>
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    </feature>
    <feature type="helix" evidence="12">
        <location>
            <begin position="72"/>
            <end position="79"/>
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    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="87"/>
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    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="94"/>
            <end position="112"/>
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    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="129"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="helix" evidence="12">
        <location>
            <begin position="136"/>
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    </feature>
    <feature type="turn" evidence="12">
        <location>
            <begin position="147"/>
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    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="151"/>
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    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="161"/>
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    <feature type="strand" evidence="12">
        <location>
            <begin position="172"/>
            <end position="177"/>
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    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="181"/>
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    </feature>
    <feature type="strand" evidence="12">
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    <feature type="strand" evidence="12">
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    <feature type="strand" evidence="12">
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    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12535540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12675797"/>
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    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="16945955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="17011573"/>
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    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="17012780"/>
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    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="17052461"/>
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    </evidence>
    <evidence type="ECO:0000269" key="8">
        <source>
            <dbReference type="PubMed" id="18703842"/>
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    </evidence>
    <evidence type="ECO:0000269" key="9">
        <source>
            <dbReference type="PubMed" id="19054331"/>
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    </evidence>
    <evidence type="ECO:0000305" key="10"/>
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        <source>
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    <evidence type="ECO:0007829" key="12">
        <source>
            <dbReference type="PDB" id="3FDS"/>
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    </evidence>
    <sequence length="245" mass="27436" checksum="7A1B0506463DFE5E" modified="2002-03-05" version="2">MKAKVIDAVSFSYILRTVGDFLSEANFIVTKEGIRVSGIDPSRVVFLDIFLPSSYFEGFEVSQEKEIIGFKLEDVNDILKRVLKDDTLILSSNESKLTLTFDGEFTRSFELPLIQVESTQPPSVNLEFPFKAQLLTITFADIIDELSDLGEVLNIHSKENKLYFEVIGDLSTAKVELSTDNGTLLEASGADVSSSYGMEYVANTTKMRRASDSMELYFGSQIPLKLRFKLPQEGYGDFYIAPRAD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z89</accession>
    <name>Y1042_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0201 protein SSO1042</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO1042</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0201 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99256">
        <property type="entry name" value="A99256"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989187.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2NWU">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=2-145"/>
    </dbReference>
    <dbReference type="PDBsum" id="2NWU"/>
    <dbReference type="AlphaFoldDB" id="Q97Z89"/>
    <dbReference type="SMR" id="Q97Z89"/>
    <dbReference type="STRING" id="273057.SSO1042"/>
    <dbReference type="PaxDb" id="273057-SSO1042"/>
    <dbReference type="EnsemblBacteria" id="AAK41304">
        <property type="protein sequence ID" value="AAK41304"/>
        <property type="gene ID" value="SSO1042"/>
    </dbReference>
    <dbReference type="GeneID" id="1454083"/>
    <dbReference type="KEGG" id="sso:SSO1042"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1036"/>
    <dbReference type="eggNOG" id="arCOG01043">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134829_1_0_2"/>
    <dbReference type="InParanoid" id="Q97Z89"/>
    <dbReference type="PhylomeDB" id="Q97Z89"/>
    <dbReference type="EvolutionaryTrace" id="Q97Z89"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01112">
        <property type="entry name" value="UPF0201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="PAB1135-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001687">
        <property type="entry name" value="PRK00447.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39652">
        <property type="entry name" value="UPF0201 PROTEIN TK1335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39652:SF1">
        <property type="entry name" value="UPF0201 PROTEIN TK1335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000094519" description="UPF0201 protein SSO1042">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="3"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="16"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="30"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="35"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="41"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="50"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="54"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="79"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="88"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="110"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="119"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="134"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01112"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="2NWU"/>
        </source>
    </evidence>
    <sequence length="145" mass="16423" checksum="D4CD325A1BC799F0" modified="2001-10-01" version="1">MVKVMVVAEVRPSEDVNKVLSAISNFFDFEKMNTRKEGIIDILVLEARTLKSLLKFHRVLRNERILDSARKYLMKGIEGNTIAFMIHKQAAAVGVLSFVDSDKESPLGAIKFYIEYQNPKEIVDWLAPKTAHGVPLWDNPVPPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZE1</accession>
    <name>Q97ZE1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calcium binding protein SSO6904 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO6904</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2010" name="Proteins" volume="78" first="474" last="479">
            <title>Solution structure and calcium binding of protein SSO6904 from the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Feng Y."/>
                <person name="Yao H."/>
                <person name="Yao H."/>
                <person name="Wang J."/>
            </authorList>
            <dbReference type="PubMed" id="19768683"/>
            <dbReference type="DOI" id="10.1002/prot.22580"/>
        </citation>
        <scope>STRUCTURE BY NMR OF 4-94</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90249">
        <property type="entry name" value="D90249"/>
    </dbReference>
    <dbReference type="PDB" id="2KJG">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=4-94"/>
    </dbReference>
    <dbReference type="PDBsum" id="2KJG"/>
    <dbReference type="AlphaFoldDB" id="Q97ZE1"/>
    <dbReference type="SMR" id="Q97ZE1"/>
    <dbReference type="STRING" id="273057.SSO6904"/>
    <dbReference type="PaxDb" id="273057-SSO6904"/>
    <dbReference type="EnsemblBacteria" id="AAK41251">
        <property type="protein sequence ID" value="AAK41251"/>
        <property type="gene ID" value="SSO6904"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO6904"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.976"/>
    <dbReference type="eggNOG" id="arCOG07215">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184956_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZE1"/>
    <dbReference type="EvolutionaryTrace" id="Q97ZE1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040534">
        <property type="entry name" value="Ca_bind_SSO6904"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18249">
        <property type="entry name" value="Ca_bind_SSO6904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcium binding protein SSO6904" evidence="1">
        <location>
            <begin position="4"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2KJG"/>
        </source>
    </evidence>
    <sequence length="94" mass="11162" checksum="FCDB75A7BE353E15" modified="2001-10-01" version="1">MMAMSILEDPEFVKLRQFKGKVNFNLVMQILDEIELDLRGSDNIKTSIIYVYSSHLDEIRKNKEFYDMIAEILQRYYKKIGIENVNQLILTTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZL4</accession>
    <name>Q97ZL4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO6716</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="G90238"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992297.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZL4"/>
    <dbReference type="STRING" id="273057.SSO6716"/>
    <dbReference type="PaxDb" id="273057-SSO6716"/>
    <dbReference type="EnsemblBacteria" id="AAK41166">
        <property type="protein sequence ID" value="AAK41166"/>
        <property type="gene ID" value="SSO6716"/>
    </dbReference>
    <dbReference type="GeneID" id="1455133"/>
    <dbReference type="KEGG" id="sso:SSO6716"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.887"/>
    <dbReference type="eggNOG" id="arCOG07220">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2393032_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZL4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="93" mass="10843" checksum="377FE8FC2CF5B3AC" modified="2001-10-01" version="1">MNPFVVKLTGRILKITSNLEYPVRVLEIILKYNVTVNMIDDKVGLKAITDSVKVNKELRKNESLEIETRLEDISEISIIYRDTMMVRRFDISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZM2</accession>
    <name>Q97ZM2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4443 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0870</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90237">
        <property type="entry name" value="G90237"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992289.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZM2"/>
    <dbReference type="SMR" id="Q97ZM2"/>
    <dbReference type="STRING" id="273057.SSO0870"/>
    <dbReference type="PaxDb" id="273057-SSO0870"/>
    <dbReference type="EnsemblBacteria" id="AAK41158">
        <property type="protein sequence ID" value="AAK41158"/>
        <property type="gene ID" value="SSO0870"/>
    </dbReference>
    <dbReference type="GeneID" id="1455125"/>
    <dbReference type="KEGG" id="sso:SSO0870"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.879"/>
    <dbReference type="eggNOG" id="arCOG02103">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112304_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZM2"/>
    <dbReference type="PhylomeDB" id="Q97ZM2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029349">
        <property type="entry name" value="DUF4443"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14544">
        <property type="entry name" value="DUF4443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="GAD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4443" evidence="5">
        <location>
            <begin position="110"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="206" mass="23098" checksum="7B6123A4F042B22E" modified="2001-10-01" version="1">MDIQIVLTKAVETRQGNKPKFDEGHVIMSLFYISQLQPVGRILLMKKTGLSEASIKTLLKRLRELNLIQTDLVGGNTLTEQGNKIVSCIKNISTIKDVTLKSLGWNSSMLIVRGGAELLKEISVLHLRDEIIRLGAEKILVCVYTEEGKIEIPPKTEEMSLRGLLDEIKENCNNCGPNDLIIFLVPNEEHLGYLTLTFILKVLKKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZN7</accession>
    <name>Q97ZN7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0847</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90235">
        <property type="entry name" value="F90235"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923136.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZN7"/>
    <dbReference type="STRING" id="273057.SSO0847"/>
    <dbReference type="PaxDb" id="273057-SSO0847"/>
    <dbReference type="EnsemblBacteria" id="AAK41141">
        <property type="protein sequence ID" value="AAK41141"/>
        <property type="gene ID" value="SSO0847"/>
    </dbReference>
    <dbReference type="GeneID" id="1455108"/>
    <dbReference type="KEGG" id="sso:SSO0847"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.863"/>
    <dbReference type="eggNOG" id="arCOG07251">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2021612_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZN7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007394">
        <property type="entry name" value="UPF0122"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04297">
        <property type="entry name" value="UPF0122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="19"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="122" mass="14216" checksum="5DDDC8649D825875" modified="2001-10-01" version="1">MSFGFYYAGIITKADAIRIIDLLNKKGLSKSEIANRIGITRSSIYQWIWDIVKDIDYENKVKLLDLFYEIDKFEAINFIEEILKEHLKILEKEKEINFKPTIKVEKILVPASTTVQLGVVDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZR6</accession>
    <name>Q97ZR6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO6008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90197">
        <property type="entry name" value="D90197"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZR6"/>
    <dbReference type="STRING" id="273057.SSO6008"/>
    <dbReference type="PaxDb" id="273057-SSO6008"/>
    <dbReference type="EnsemblBacteria" id="AAK40835">
        <property type="protein sequence ID" value="AAK40835"/>
        <property type="gene ID" value="SSO6008"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO6008"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.511"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2505118_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZR6"/>
    <dbReference type="PhylomeDB" id="Q97ZR6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="85" mass="10112" checksum="B33D0EA9B26E155E" modified="2001-10-01" version="1">MVNNAREYVNNPLKELVSLYLFMKDAQTKQENRLRRALFLVSDEEKLSEERLEKFSKGDFSDVKLYQLEYTGTVLRQYGNLIFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZR8</accession>
    <name>Q97ZR8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0512</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90196">
        <property type="entry name" value="H90196"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZR8"/>
    <dbReference type="STRING" id="273057.SSO0512"/>
    <dbReference type="PaxDb" id="273057-SSO0512"/>
    <dbReference type="EnsemblBacteria" id="AAK40831">
        <property type="protein sequence ID" value="AAK40831"/>
        <property type="gene ID" value="SSO0512"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0512"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.507"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113185_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZR8"/>
    <dbReference type="PhylomeDB" id="Q97ZR8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="245" mass="27487" checksum="25AD72CEB0BCC09F" modified="2001-10-01" version="1">MTKELTREEYYKALEKAVNEVILSMTGTRKDVAKRLVLGAVVGRNATEIAQEAEMDYETVLNNLDKAAQAKLIEVVKKLVGDHPVLLIVDDIHDHKLYARAMPVSRNGAQIFYCRAHKRFEPAIQLLVIGIKDLVNNQIYVIHIIAYIPRKVEEELKRRGEEVKFRTKIDALLEFLSSLSGLNVKSKVFDSWYVNSRTLQGNTVGELKSSARVVEGGRSVPVSEFPPRGVPGGVLGDSHKVTCYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZU9</accession>
    <name>Q97ZU9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metalloprotease TldD/E C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0478</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90192">
        <property type="entry name" value="H90192"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922993.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZU9"/>
    <dbReference type="SMR" id="Q97ZU9"/>
    <dbReference type="STRING" id="273057.SSO0478"/>
    <dbReference type="PaxDb" id="273057-SSO0478"/>
    <dbReference type="DNASU" id="1455609"/>
    <dbReference type="EnsemblBacteria" id="AAK40799">
        <property type="protein sequence ID" value="AAK40799"/>
        <property type="gene ID" value="SSO0478"/>
    </dbReference>
    <dbReference type="GeneID" id="7807119"/>
    <dbReference type="KEGG" id="sso:SSO0478"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.472"/>
    <dbReference type="eggNOG" id="arCOG00323">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_757837_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZU9"/>
    <dbReference type="PhylomeDB" id="Q97ZU9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051463">
        <property type="entry name" value="Peptidase_U62_metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624:SF0">
        <property type="entry name" value="METALLOPROTEASE SLR0863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN TLDD AND PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="2">
        <location>
            <begin position="178"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="380" mass="43645" checksum="E8BFD805E7985E3A" modified="2001-10-01" version="1">MRKLKREAYKLNNIKMLQQDVKYSEEVIEAEFSVEGYSLIDYKRSYTLSRALVNGKWIVYDGNRLESSQKIKEFEICEDFYHESVKAWPNKEKYSRLFTNIKLNEDDQLKVISKKISKSILIHDVKECREEKVVNYVEYNDLNFSYAGNPKDIPSIVEYLRASQSQLTSPSRQVLTESGRLTFILDSEIVASIFHYVLRNFLNGNSPKLKLNEKISSEITIYDNPLNSFSSSFSVFDDEGVKTKKKEIIGDGIITNYLGTLTSKYGEAGNARGILPKPDYFSLEVKQGDWSLNELIDESRGAYIAMGVKKSELIRNSIRITPRSVVKIGQGQIFFREIAIPLQELVTIDAVTRENRGVFIDEEHGGVTPYVRMKVRAILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZV3</accession>
    <name>Q97ZV3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alcohol dehydrogenase (Zn containing) (Adh-1)</fullName>
            <ecNumber evidence="8">1.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">adh-1</name>
        <name evidence="8" type="ordered locus">SSO0472</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90192">
        <property type="entry name" value="D90192"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZV3"/>
    <dbReference type="FunCoup" id="Q97ZV3">
        <property type="interactions" value="244"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0472"/>
    <dbReference type="PaxDb" id="273057-SSO0472"/>
    <dbReference type="EnsemblBacteria" id="AAK40795">
        <property type="protein sequence ID" value="AAK40795"/>
        <property type="gene ID" value="SSO0472"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0472"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.468"/>
    <dbReference type="eggNOG" id="arCOG01456">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_14_1_2"/>
    <dbReference type="InParanoid" id="Q97ZV3"/>
    <dbReference type="PhylomeDB" id="Q97ZV3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08263">
        <property type="entry name" value="Zn_ADH10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000003">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43350:SF2">
        <property type="entry name" value="GROES-LIKE ZINC-BINDING ALCOHOL DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43350">
        <property type="entry name" value="NAD-DEPENDENT ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="16"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK40795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="371" mass="39593" checksum="8516AD592720E502" modified="2001-10-01" version="1">MIILYLILMKGAILYKYNEPLIIEDNIQIDEPKVGETKIRIEATGLCHSDVNVFEGKTPVPPPVIAGHEIAGVIEEVGNNVTDFKPGDRVISAFIHPCGKCKNCITGKENLCEVFAKNRLNGTLLDGTTRLHFKDGTPIRAFLGGGFAEYAMVPYTALTKVPEDLDLRKVAVLGCAGLTAYGAVNSAKIEPGETVAVIGVGGVGLSVIQMLKIAGAGRIIAVGTRKWKLEKALELGASDVVNSKETDVVRAVKNITGGGPDVVIEVAGTTETVKMSLDMVRIGGKVVLVGLPPTTAEIPIRIASIVRGGIKIIGDYGGRPRVDMPRLIELVKLGKYDPTALVTGKFRLEEINEAVKLLEQGEAIRSLIIPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZW5</accession>
    <name>Q97ZW5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90190">
        <property type="entry name" value="H90190"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988699.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZW5"/>
    <dbReference type="STRING" id="273057.SSO0458"/>
    <dbReference type="PaxDb" id="273057-SSO0458"/>
    <dbReference type="EnsemblBacteria" id="AAK40783">
        <property type="protein sequence ID" value="AAK40783"/>
        <property type="gene ID" value="SSO0458"/>
    </dbReference>
    <dbReference type="GeneID" id="1455593"/>
    <dbReference type="KEGG" id="sso:SSO0458"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.452"/>
    <dbReference type="eggNOG" id="arCOG02242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150444_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZW5"/>
    <dbReference type="PhylomeDB" id="Q97ZW5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="19"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="116" mass="12890" checksum="AAE54CDB334DEE39" modified="2001-10-01" version="1">MSIEISEKSFLLKRFLIVAYGLSEADVDAFIKIVSSETGKDVDAIAGELGISKSRASLILKKLADAGLVEKEKTSVSRGGRPKFLYRINKEELKKKLIKRSEETCKDLHTIISSFL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-06-07" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q980A7</accession>
    <name>CBIX_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sirohydrochlorin cobaltochelatase</fullName>
            <ecNumber evidence="1">4.99.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">CbiXS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbiX</name>
        <name type="ordered locus">SSO0410</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the insertion of Co(2+) into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Co-sirohydrochlorin + 2 H(+) = sirohydrochlorin + Co(2+)</text>
            <dbReference type="Rhea" id="RHEA:15893"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:60049"/>
            <dbReference type="EC" id="4.99.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 1/10.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CbiX family. CbiXS subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.99.1.3" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90185">
        <property type="entry name" value="C90185"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988774.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980A7"/>
    <dbReference type="SMR" id="Q980A7"/>
    <dbReference type="FunCoup" id="Q980A7">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0410"/>
    <dbReference type="PaxDb" id="273057-SSO0410"/>
    <dbReference type="EnsemblBacteria" id="AAK40738">
        <property type="protein sequence ID" value="AAK40738"/>
        <property type="gene ID" value="SSO0410"/>
    </dbReference>
    <dbReference type="GeneID" id="1455548"/>
    <dbReference type="KEGG" id="sso:SSO0410"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.405"/>
    <dbReference type="eggNOG" id="arCOG02246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065901_2_1_2"/>
    <dbReference type="InParanoid" id="Q980A7"/>
    <dbReference type="PhylomeDB" id="Q980A7"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00223"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016852">
        <property type="term" value="F:sirohydrochlorin cobaltochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03416">
        <property type="entry name" value="CbiX_SirB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00785">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002762">
        <property type="entry name" value="CbiX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023652">
        <property type="entry name" value="SiroHydchlorin_Cochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050963">
        <property type="entry name" value="Sirohydro_Cobaltochel/CbiX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33542">
        <property type="entry name" value="SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33542:SF3">
        <property type="entry name" value="SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01903">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000150362" description="Sirohydrochlorin cobaltochelatase">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="68"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00785"/>
        </source>
    </evidence>
    <sequence length="128" mass="14606" checksum="AEB6788BFA35775C" modified="2001-10-01" version="1">MLGVLLVLHGSKIPEWKDVGIKYAEYLSKYFSLVEFGFLEFNKPTLSEALSNLLAKGADKIVVVPLLFATGTHFRRDIPRLLGIDNDEKKIRYMGREIEITIADPLGFDEKIGEVLVKRVNETYNKNY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2014-06-11" modified="2026-01-28" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>Q980D2</accession>
    <name>MVK_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mevalonate kinase</fullName>
            <shortName evidence="1">MK</shortName>
            <shortName evidence="1">MVK</shortName>
            <ecNumber evidence="1">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mvk</name>
        <name type="ordered locus">SSO0383</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2013" name="J. Biochem." volume="153" first="415" last="420">
            <title>Biochemical evidence supporting the presence of the classical mevalonate pathway in the thermoacidophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Nishimura H."/>
                <person name="Azami Y."/>
                <person name="Miyagawa M."/>
                <person name="Hashimoto C."/>
                <person name="Yoshimura T."/>
                <person name="Hemmi H."/>
            </authorList>
            <dbReference type="PubMed" id="23378249"/>
            <dbReference type="DOI" id="10.1093/jb/mvt006"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PATHWAY</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the phosphorylation of (R)-mevalonate (MVA) to (R)-mevalonate 5-phosphate (MVAP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds such as archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 2">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90181">
        <property type="entry name" value="H90181"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922957.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980D2"/>
    <dbReference type="SMR" id="Q980D2"/>
    <dbReference type="FunCoup" id="Q980D2">
        <property type="interactions" value="133"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0383"/>
    <dbReference type="PaxDb" id="273057-SSO0383"/>
    <dbReference type="EnsemblBacteria" id="AAK40711">
        <property type="protein sequence ID" value="AAK40711"/>
        <property type="gene ID" value="SSO0383"/>
    </dbReference>
    <dbReference type="GeneID" id="1455521"/>
    <dbReference type="GeneID" id="44129357"/>
    <dbReference type="KEGG" id="sso:SSO0383"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.377"/>
    <dbReference type="eggNOG" id="arCOG01028">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017814_0_0_2"/>
    <dbReference type="InParanoid" id="Q980D2"/>
    <dbReference type="PhylomeDB" id="Q980D2"/>
    <dbReference type="BRENDA" id="2.7.1.36">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00217">
        <property type="entry name" value="Mevalonate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022937">
        <property type="entry name" value="Mevalonate_kinase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290:SF2">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000429457" description="Mevalonate kinase">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="103"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="23378249"/>
        </source>
    </evidence>
    <sequence length="314" mass="34542" checksum="B2E968C32ED15117" modified="2001-10-01" version="1">MMVEAKVPLKLTLFGEHAVVYDRPAIAMTISESLKVKVSENDKFLIISPSLNIKGVKLDLNEMKIESDEAKKVLRYVFEVLNYFEMKKPVKIEINSTVEPSVGLGTSAAVIVGTVAAYSKYLGIDLSRDEIAKISHNIELKVQGIASRMDTYTETYGGLIYFPAGGKGFEKIDTNFELTAGYIRRSMSTADVLWRVRTLKESNKEVFENILDVIGEITNRAKSLIVEQNFEELGLLMYVNHGLLFSLGITSPEADEIVSRAKQLGIKGCKISGGGAGGSIICIKSVEAEVLLRSYNARIVNSTLTKDGVIFSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="146" xmlns="https://uniprot.org/uniprot">
    <accession>Q980E5</accession>
    <name>Q980E5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thioredoxin (TrxA-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">trxA-1</name>
        <name evidence="6" type="ordered locus">SSO0368</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="J. Struct. Biol." volume="177" first="506" last="512">
            <title>Crystallographic and spectroscopic characterizations of Sulfolobus solfataricus TrxA1 provide insights into the determinants of thioredoxin fold stability.</title>
            <authorList>
                <person name="Esposito L."/>
                <person name="Ruggiero A."/>
                <person name="Masullo M."/>
                <person name="Ruocco M.R."/>
                <person name="Lamberti A."/>
                <person name="Arcari P."/>
                <person name="Zagari A."/>
                <person name="Vitagliano L."/>
            </authorList>
            <dbReference type="PubMed" id="22085748"/>
            <dbReference type="DOI" id="10.1016/j.jsb.2011.10.014"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 25-133</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="journal article" date="2019" name="FEBS J." volume="286" first="1752" last="1764">
            <title>Loop size optimization induces a strong thermal stabilization of the thioredoxin fold.</title>
            <authorList>
                <person name="Ruggiero A."/>
                <person name="Smaldone G."/>
                <person name="Esposito L."/>
                <person name="Balasco N."/>
                <person name="Vitagliano L."/>
            </authorList>
            <dbReference type="PubMed" id="30675750"/>
            <dbReference type="DOI" id="10.1111/febs.14767"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.99 ANGSTROMS) OF 40-47</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90180">
        <property type="entry name" value="C90180"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922953.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3TCO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A/B/C=25-133"/>
    </dbReference>
    <dbReference type="PDB" id="6H1Y">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.99 A"/>
        <property type="chains" value="A/B=40-47"/>
    </dbReference>
    <dbReference type="PDBsum" id="3TCO"/>
    <dbReference type="PDBsum" id="6H1Y"/>
    <dbReference type="AlphaFoldDB" id="Q980E5"/>
    <dbReference type="SMR" id="Q980E5"/>
    <dbReference type="FunCoup" id="Q980E5">
        <property type="interactions" value="260"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0368"/>
    <dbReference type="PaxDb" id="273057-SSO0368"/>
    <dbReference type="EnsemblBacteria" id="AAK40698">
        <property type="protein sequence ID" value="AAK40698"/>
        <property type="gene ID" value="SSO0368"/>
    </dbReference>
    <dbReference type="GeneID" id="1455508"/>
    <dbReference type="KEGG" id="sso:SSO0368"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.362"/>
    <dbReference type="eggNOG" id="arCOG01972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_10_1_2"/>
    <dbReference type="InParanoid" id="Q980E5"/>
    <dbReference type="PhylomeDB" id="Q980E5"/>
    <dbReference type="EvolutionaryTrace" id="Q980E5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000648">
        <property type="entry name" value="Thioredoxin (TrxA-1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663:SF11">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="8 9" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="20"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="57"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="3TCO"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="6H1Y"/>
        </source>
    </evidence>
    <sequence length="133" mass="15228" checksum="370828E13268CBCC" modified="2001-10-01" version="1">MSEIDSLIKEVAERLQKKAEEYGKKKEDVTLVLTEENFDEVIRNNKLVLVDCWAEWCAPCHLYEPIYKKVAEKYKGKAVFGRLNVDENQKIADKYSVLNIPTTLIFVNGQLVDSLVGAVDEDTLESTVNKYLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q980I8</accession>
    <name>AROE_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Shikimate dehydrogenase (NADP(+))</fullName>
            <shortName evidence="1">SDH</shortName>
            <ecNumber evidence="1">1.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroE</name>
        <name type="ordered locus">SSO0306</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>shikimate + NADP(+) = 3-dehydroshikimate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.25" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90173">
        <property type="entry name" value="D90173"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990598.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980I8"/>
    <dbReference type="SMR" id="Q980I8"/>
    <dbReference type="STRING" id="273057.SSO0306"/>
    <dbReference type="PaxDb" id="273057-SSO0306"/>
    <dbReference type="EnsemblBacteria" id="AAK40643">
        <property type="protein sequence ID" value="AAK40643"/>
        <property type="gene ID" value="SSO0306"/>
    </dbReference>
    <dbReference type="GeneID" id="1455453"/>
    <dbReference type="KEGG" id="sso:SSO0306"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.299"/>
    <dbReference type="eggNOG" id="arCOG01033">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044063_4_1_2"/>
    <dbReference type="InParanoid" id="Q980I8"/>
    <dbReference type="PhylomeDB" id="Q980I8"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01065">
        <property type="entry name" value="NAD_bind_Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00222">
        <property type="entry name" value="Shikimate_DH_AroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089:SF1">
        <property type="entry name" value="BIFUNCTIONAL 3-DEHYDROQUINATE DEHYDRATASE_SHIKIMATE DEHYDROGENASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="SHIKIMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000021355" description="Shikimate dehydrogenase (NADP(+))">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="22"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="128"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="152"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00222"/>
        </source>
    </evidence>
    <sequence length="269" mass="30472" checksum="386002ACFF7E4E88" modified="2001-10-01" version="1">MLEEINYDTKLFGLIGKNIKYTLSPYIHNFSFRTLGINAVYLVFDLDEMKFKRSISGILEIAEGLNVTIPYKDEVMKYLDNTDTHSTRIQAVNTIYKKSGYNTDYLAIKNLVRKKIKNVSGYECYIYGAGGAAKAAAFALSELGCSSISIVNRTKSRAYELAELLNKNGYNASIKENCNITNNILIVNSTPNSSVVPEDCVKKSDLVIEFVYRPVETELIKNAKKYGIQYINGLEILVNQAVEAEKIWFNKSVADEKIIEYLYARELVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q980L9</accession>
    <name>Q980L9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxylate--amine ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0273</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90169">
        <property type="entry name" value="D90169"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980L9"/>
    <dbReference type="SMR" id="Q980L9"/>
    <dbReference type="STRING" id="273057.SSO0273"/>
    <dbReference type="PaxDb" id="273057-SSO0273"/>
    <dbReference type="EnsemblBacteria" id="AAK40611">
        <property type="protein sequence ID" value="AAK40611"/>
        <property type="gene ID" value="SSO0273"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0273"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.267"/>
    <dbReference type="eggNOG" id="arCOG00347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2313919_0_0_2"/>
    <dbReference type="InParanoid" id="Q980L9"/>
    <dbReference type="PhylomeDB" id="Q980L9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11619" checksum="427E906563C3E5F7" modified="2001-10-01" version="1">MSNVKIVIKKDLSELKGSTFITGFRTIGEVGYLAIRHLALKRKMERIGYVVTKYYRDVTFLDDYGIATPFDIFYDKDKHLVLLLNHILPFQREWNDFAS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-06" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q980N1</accession>
    <name>GGGPS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Geranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="1">GGGP synthase</shortName>
            <shortName evidence="1">GGGPS</shortName>
            <ecNumber evidence="1 2">2.5.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">(S)-3-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoglycerol geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0259</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="J. Biol. Chem." volume="279" first="50197" last="50203">
            <title>(S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase from the thermoacidophilic archaeon Sulfolobus solfataricus. Molecular cloning and characterization of a membrane-intrinsic prenyltransferase involved in the biosynthesis of archaeal ether-linked membrane lipids.</title>
            <authorList>
                <person name="Hemmi H."/>
                <person name="Shibuya K."/>
                <person name="Takahashi Y."/>
                <person name="Nakayama T."/>
                <person name="Nishino T."/>
            </authorList>
            <dbReference type="PubMed" id="15356000"/>
            <dbReference type="DOI" id="10.1074/jbc.M409207200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>sn-glycerol 1-phosphate + (2E,6E,10E)-geranylgeranyl diphosphate = sn-3-O-(geranylgeranyl)glycerol 1-phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23404"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="EC" id="2.5.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GGGP/HepGP synthase family. Group II subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.41" evidence="1 2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90167">
        <property type="entry name" value="H90167"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922925.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980N1"/>
    <dbReference type="SMR" id="Q980N1"/>
    <dbReference type="STRING" id="273057.SSO0259"/>
    <dbReference type="PaxDb" id="273057-SSO0259"/>
    <dbReference type="EnsemblBacteria" id="AAK40599">
        <property type="protein sequence ID" value="AAK40599"/>
        <property type="gene ID" value="SSO0259"/>
    </dbReference>
    <dbReference type="GeneID" id="1455409"/>
    <dbReference type="KEGG" id="sso:SSO0259"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.255"/>
    <dbReference type="eggNOG" id="arCOG01085">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068610_0_0_2"/>
    <dbReference type="InParanoid" id="Q980N1"/>
    <dbReference type="PhylomeDB" id="Q980N1"/>
    <dbReference type="BRENDA" id="1.1.1.34">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120536">
        <property type="term" value="F:heptaprenylglyceryl phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047294">
        <property type="term" value="F:phosphoglycerol geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02812">
        <property type="entry name" value="PcrB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.390:FF:000001">
        <property type="entry name" value="Heptaprenylglyceryl phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.390">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00112">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039074">
        <property type="entry name" value="GGGP/HepGP_synthase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038597">
        <property type="entry name" value="GGGP/HepGP_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008205">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010946">
        <property type="entry name" value="GGGP_synth"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01769">
        <property type="entry name" value="GGGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01768">
        <property type="entry name" value="GGGP-family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003198">
        <property type="entry name" value="PRK04169.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003202">
        <property type="entry name" value="PRK04169.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40029">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40029:SF2">
        <property type="entry name" value="HEPTAPRENYLGLYCERYL PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01884">
        <property type="entry name" value="PcrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000138744" description="Geranylgeranylglyceryl phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="182"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="213"/>
            <end position="214"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="235"/>
            <end position="236"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15356000"/>
        </source>
    </evidence>
    <sequence length="255" mass="27921" checksum="AB0B8E7A187591B6" modified="2001-10-01" version="1">MRILKKKMKLKGKVKKYLIDKLNDNEKLHFSLIDPFKINSSDELKYITKNLYNAGTDAFLIGGTLGVSKDKLDFVISLLDDYEIPKIIFPSNINLLSEKADALLFMSLLNSDDIYYIIGAQIVAAPIIKMLQMEVIPTAYVIVGHGGTAAHIGKARVIPYDNFELATAYTLAAEYLGMSFVYLEAGSGAPEPIRPEMISFIKNASSIPLIVGGGIRSVEVALKLVEAGADIIVTGNIIESDVNKAIKIIRGIKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q980P1</accession>
    <name>Q980P1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">threonine ammonia-lyase</fullName>
            <ecNumber evidence="3">4.3.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tdcB</name>
        <name evidence="7" type="ordered locus">SSO0248</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90166">
        <property type="entry name" value="D90166"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990508.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980P1"/>
    <dbReference type="FunCoup" id="Q980P1">
        <property type="interactions" value="270"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0248"/>
    <dbReference type="PaxDb" id="273057-SSO0248"/>
    <dbReference type="EnsemblBacteria" id="AAK40587">
        <property type="protein sequence ID" value="AAK40587"/>
        <property type="gene ID" value="SSO0248"/>
    </dbReference>
    <dbReference type="GeneID" id="1455397"/>
    <dbReference type="KEGG" id="sso:SSO0248"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.242"/>
    <dbReference type="eggNOG" id="arCOG01431">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021152_4_1_2"/>
    <dbReference type="InParanoid" id="Q980P1"/>
    <dbReference type="PhylomeDB" id="Q980P1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04886">
        <property type="entry name" value="ACT_ThrD-II-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000007">
        <property type="entry name" value="L-threonine dehydratase catabolic TdcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044561">
        <property type="entry name" value="ACT_ThrD-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005789">
        <property type="entry name" value="Thr_deHydtase_catblc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01127">
        <property type="entry name" value="ilvA_1Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="6">
        <location>
            <begin position="330"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="405" mass="44364" checksum="F1266A940110B062" modified="2001-10-01" version="1">MNYLEYFDRIRLAKEKIEKYVHITPIDYSTTFSRIINAKVYLKLENLQKTGSFKVRGAFNKLLSLKEEEKKNGVIAVSAGNHAQGVAYAASTLNIKSTIVMPETAPASKYLATKSYGAEVVLYGKYLHESMKKAEELIQNTGLIFVHPYSDLDVITGQGTIGLELYDIEPDYVIIPIGGGGLISGISIALKYRFPNVKIIGVQSSSSPSMKVSKDLGRLVEIEPSYSIADGILVKSPSELTFSIINELVDDIVLVDDEEIAEAIVLLLERSKTLAEGAGAAALASLISGKVKVNGIDKKVISLVSGGNIDLSLLSTLTEKFLYRQKRVVKVRVIVPDKPGQLNKVLSYVVKIRGNIIDIVHDRHSSDVLPGYTKIYITFELQSSEAITLLLTNLANEGIDVKIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q980P3</accession>
    <name>Q980P3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">siroheme decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.111</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nirD</name>
        <name evidence="8" type="ordered locus">SSO0245</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>siroheme + 2 H(+) = 12,18-didecarboxysiroheme + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:19093"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
            <dbReference type="ChEBI" id="CHEBI:140497"/>
            <dbReference type="EC" id="4.1.1.111"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ahb/Nir family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.111" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90166">
        <property type="entry name" value="B90166"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990502.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980P3"/>
    <dbReference type="SMR" id="Q980P3"/>
    <dbReference type="STRING" id="273057.SSO0245"/>
    <dbReference type="PaxDb" id="273057-SSO0245"/>
    <dbReference type="DNASU" id="1455395"/>
    <dbReference type="EnsemblBacteria" id="AAK40585">
        <property type="protein sequence ID" value="AAK40585"/>
        <property type="gene ID" value="SSO0245"/>
    </dbReference>
    <dbReference type="GeneID" id="1455395"/>
    <dbReference type="KEGG" id="sso:SSO0245"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.240"/>
    <dbReference type="eggNOG" id="arCOG01628">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049427_1_0_2"/>
    <dbReference type="InParanoid" id="Q980P3"/>
    <dbReference type="PhylomeDB" id="Q980P3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3460">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040523">
        <property type="entry name" value="AsnC_trans_reg2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053953">
        <property type="entry name" value="NirdL-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF1">
        <property type="entry name" value="SIROHEME DECARBOXYLASE NIRL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17805">
        <property type="entry name" value="AsnC_trans_reg2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22451">
        <property type="entry name" value="NirdL-like_HTH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Siroheme decarboxylase NirL-like HTH" evidence="7">
        <location>
            <begin position="10"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme decarboxylase AsnC-like ligand binding" evidence="6">
        <location>
            <begin position="68"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme decarboxylase NirL-like HTH" evidence="7">
        <location>
            <begin position="182"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme decarboxylase AsnC-like ligand binding" evidence="6">
        <location>
            <begin position="237"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK40585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="327" mass="37728" checksum="BE53EA618FF18A9B" modified="2001-10-01" version="1">MNLVEISDIDKEILMKLEYEFPFDPQPFNIISDGLKISEEELLNRVKKLLEDGIIKRIGMYVSFRAKGMDGALIAARIPQDKIEKFRKIALGIRELTHNYVRDHPKFNIWFVLKANDRDTLNENVKELLSHVESNDYVILYSKKTLKLSVKYDVIRGVSWSPNNTIRSLSKVPTAEELGINKQLLTELSYPLKISQRPFKEIAEKYKMKEDELVELIKELYDKNVIKDYGATLNGEKIGIVENGMVLLDTSNVEQACEKLALNIPEATHVVLRESNDENWKYLCYSMIHASKKETIRNVAKEISKETNSRSYMILFSLENLKPGIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q980V6</accession>
    <name>Q980V6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleotidyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90157">
        <property type="entry name" value="E90157"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980V6"/>
    <dbReference type="STRING" id="273057.SSO0171"/>
    <dbReference type="PaxDb" id="273057-SSO0171"/>
    <dbReference type="DNASU" id="1453343"/>
    <dbReference type="EnsemblBacteria" id="AAK40516">
        <property type="protein sequence ID" value="AAK40516"/>
        <property type="gene ID" value="SSO0171"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0171"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.167"/>
    <dbReference type="eggNOG" id="arCOG04119">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120784_0_0_2"/>
    <dbReference type="InParanoid" id="Q980V6"/>
    <dbReference type="PhylomeDB" id="Q980V6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018700">
        <property type="entry name" value="DUF2204"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09970">
        <property type="entry name" value="DUF2204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="21870" checksum="EEB3D73B9609A77E" modified="2001-10-01" version="1">MHMLIPFSKVGEVLHELQDLTKFIIIGDTIVDITLKRKGTESDVDLFALDISVLVDDDKIRNFAYERGWDYGRTPIDTPRLLAPIDDDQLQIDLYENIQDFFVPKEILESAINIKIGGYEFKSIRLEDYILLKANAFREEDEDELKTLLYLLGEGKISIDKEYLKEHIQAFEENSESIKERLSSIGIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Y1</accession>
    <name>Q980Y1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90154">
        <property type="entry name" value="D90154"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922899.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Y1"/>
    <dbReference type="PaxDb" id="273057-SSO0141"/>
    <dbReference type="EnsemblBacteria" id="AAK40491">
        <property type="protein sequence ID" value="AAK40491"/>
        <property type="gene ID" value="SSO0141"/>
    </dbReference>
    <dbReference type="GeneID" id="1453318"/>
    <dbReference type="KEGG" id="sso:SSO0141"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.133"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="InParanoid" id="Q980Y1"/>
    <dbReference type="PhylomeDB" id="Q980Y1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="80"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37386" checksum="C463C16A7C7E5DE7" modified="2001-10-01" version="1">MQTMILPKTELKSLAITLATNNINVISQDLDPEIVKAAPSLLTGNRGKYYLKVVRRGEKVISKGQRTFKFYPIYREVKGETNVVAVDETGLTVGEKEQEKAEGFLLYNWKRKGVKMRSLDLVYPLRLPLLVEVADLRSDSPSQFLLRSVREVSQYMEIDYVVADAGFLNLGVIKEMPVKTIVRGKSNLKGFKELSNVPLVEKRYEVKDKVYVAYRVLEFEGLYYYDVVYVKGKPRHFMFVTNFEGDPYELAELYRLRWQVEEGFKVRKARIRYVRKLSNKIFLFLYYTVLDSAWNLVNHLLFNFKSTCKKVLSFDSFVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Z2</accession>
    <name>Q980Z2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">4-hydroxybenzoate octaprenyltransferase (UbiA-1)</fullName>
            <ecNumber evidence="9">2.5.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ubiA-1</name>
        <name evidence="9" type="ordered locus">SSO0125</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="9"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90153">
        <property type="entry name" value="A90153"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988950.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Z2"/>
    <dbReference type="FunCoup" id="Q980Z2">
        <property type="interactions" value="192"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0125"/>
    <dbReference type="PaxDb" id="273057-SSO0125"/>
    <dbReference type="DNASU" id="1453307"/>
    <dbReference type="EnsemblBacteria" id="AAK40480">
        <property type="protein sequence ID" value="AAK40480"/>
        <property type="gene ID" value="SSO0125"/>
    </dbReference>
    <dbReference type="GeneID" id="1453307"/>
    <dbReference type="KEGG" id="sso:SSO0125"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.121"/>
    <dbReference type="eggNOG" id="arCOG00477">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034879_5_1_2"/>
    <dbReference type="InParanoid" id="Q980Z2"/>
    <dbReference type="PhylomeDB" id="Q980Z2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000008">
        <property type="entry name" value="4-hydroxybenzoate octaprenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006371">
        <property type="entry name" value="Polyprenyltransferase_UbiA-li"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009609">
        <property type="entry name" value="PRK13106.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01475">
        <property type="entry name" value="ubiA_other"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048:SF28">
        <property type="entry name" value="4-HYDROXYBENZOATE POLYPRENYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PRENYLTRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK40480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="302" mass="33924" checksum="3568065FDD7C4E5F" modified="2001-10-01" version="1">MSWDPGGLNKNTRNKLYIILRFLRIEQTFFSLPMAYLGAFVAIKGIPPIPTLILIFLALFFLRTAGMTNDNLADIEIDAKNPRTKNRPLVTGAIKIREARTMIVASLILFFITSYFVNIWAFILAPIVALIIMSYPYMKRYTAFANYHLASIQGLAVFSGAVAVLGLYYNSLDQILLRVPWFFVIGTILWAVGFDLYNHIPDAEFDKKMGLHSFAVVLGRWALTFAGLNQLFSVVLDLLGDFYYGLGPIAIFATVLHGLIMAYAYYLASQKNDFGRAFYYNIYSSIVLGLGVDIDVILGIPF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-25" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXC0</accession>
    <name>RRP42_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Exosome complex component Rrp42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rrp42</name>
        <name type="ordered locus">SSO0732</name>
        <name type="ORF">C20_023</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
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            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
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            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
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            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
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        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="EMBO Rep." volume="4" first="889" last="893">
            <title>An exosome-like complex in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Evguenieva-Hackenberg E."/>
                <person name="Walter P."/>
                <person name="Hochleitner E."/>
                <person name="Lottspeich F."/>
                <person name="Klug G."/>
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            <dbReference type="DOI" id="10.1038/sj.embor.embor929"/>
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        <scope>INTERACTION WITH EXOSOME</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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    </reference>
    <reference key="4">
        <citation type="journal article" date="2006" name="Mol. Microbiol." volume="62" first="1076" last="1089">
            <title>Characterization of native and reconstituted exosome complexes from the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
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                <person name="Walter P."/>
                <person name="Klein F."/>
                <person name="Lorentzen E."/>
                <person name="Ilchmann A."/>
                <person name="Klug G."/>
                <person name="Evguenieva-Hackenberg E."/>
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    <reference key="5">
        <citation type="journal article" date="2010" name="FEBS Lett." volume="584" first="2931" last="2936">
            <title>The evolutionarily conserved subunits Rrp4 and Csl4 confer different substrate specificities to the archaeal exosome.</title>
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                <person name="Roppelt V."/>
                <person name="Klug G."/>
                <person name="Evguenieva-Hackenberg E."/>
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            <dbReference type="DOI" id="10.1016/j.febslet.2010.05.014"/>
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    <reference key="6">
        <citation type="journal article" date="2012" name="Biochimie" volume="94" first="1578" last="1587">
            <title>Heterogeneous complexes of the RNA exosome in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Witharana C."/>
                <person name="Roppelt V."/>
                <person name="Lochnit G."/>
                <person name="Klug G."/>
                <person name="Evguenieva-Hackenberg E."/>
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            <dbReference type="DOI" id="10.1016/j.biochi.2012.03.026"/>
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        <scope>INTERACTION WITH EXOSOME</scope>
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            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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    <reference key="7">
        <citation type="journal article" date="2005" name="Mol. Cell" volume="20" first="473" last="481">
            <title>Structural basis of 3' end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core.</title>
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                <person name="Lorentzen E."/>
                <person name="Conti E."/>
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        <scope>X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) IN COMPLEX WITH RRP41</scope>
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    <reference key="8">
        <citation type="journal article" date="2005" name="Nat. Struct. Mol. Biol." volume="12" first="575" last="581">
            <title>The archaeal exosome core is a hexameric ring structure with three catalytic subunits.</title>
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                <person name="Lorentzen E."/>
                <person name="Walter P."/>
                <person name="Fribourg S."/>
                <person name="Evguenieva-Hackenberg E."/>
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        <citation type="journal article" date="2007" name="EMBO Rep." volume="8" first="470" last="476">
            <title>RNA channelling by the archaeal exosome.</title>
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                <person name="Lorentzen E."/>
                <person name="Dziembowski A."/>
                <person name="Lindner D."/>
                <person name="Seraphin B."/>
                <person name="Conti E."/>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E8739" last="E8739">
            <title>Crystal structure of the S. solfataricus archaeal exosome reveals conformational flexibility in the RNA-binding ring.</title>
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                <person name="Lu C."/>
                <person name="Ding F."/>
                <person name="Ke A."/>
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        <citation type="journal article" date="2012" name="Archaea" volume="2012" first="721869" last="721869">
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    </feature>
    <feature type="turn" evidence="13">
        <location>
            <begin position="231"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="248"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15951817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="16285928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="17380186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="20090900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="20488184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="23319881"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="2C37"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="2C38"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="2JE6"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="11">
        <source>
            <dbReference type="PDB" id="2JEA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="12">
        <source>
            <dbReference type="PDB" id="2JEB"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="13">
        <source>
            <dbReference type="PDB" id="3L7Z"/>
        </source>
    </evidence>
    <sequence length="275" mass="30194" checksum="4188C9D6928A144C" modified="2000-05-01" version="1">MSSTPSNQNIIPIIKKESIVSLFEKGIRQDGRKLTDYRPLSITLDYAKKADGSALVKLGTTMVLAGTKLEIDKPYEDTPNQGNLIVNVELLPLAYETFEPGPPDENAIELARVVDRSLRDSKALDLTKLVIEPGKSVWTVWLDVYVLDYGGNVLDACTLASVAALYNTKVYKVEQHSNGISVNKNEVVGKLPLNYPVVTISVAKVDKYLVVDPDLDEESIMDAKISFSYTPDLKIVGIQKSGKGSMSLQDIDQAENTARSTAVKLLEELKKHLGI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2026-01-28" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXF8</accession>
    <name>CDC62_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ORC1-type DNA replication protein 2</fullName>
            <ecNumber evidence="2">3.6.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">SsoCdc6-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cdc6-2</name>
        <name type="ordered locus">SSO0771</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="J. Biol. Chem." volume="278" first="46424" last="46431">
            <title>Biochemical characterization of a CDC6-like protein from the crenarchaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="De Felice M."/>
                <person name="Esposito L."/>
                <person name="Pucci B."/>
                <person name="Carpentieri F."/>
                <person name="De Falco M."/>
                <person name="Rossi M."/>
                <person name="Pisani F.M."/>
            </authorList>
            <dbReference type="PubMed" id="12966100"/>
            <dbReference type="DOI" id="10.1074/jbc.M306075200"/>
        </citation>
        <scope>FUNCTION AS AN ATPASE</scope>
        <scope>SUBUNIT</scope>
        <scope>AUTOPHOSPHORYLATION</scope>
        <scope>DNA-BINDING</scope>
        <scope>LEVEL OF PROTEIN EXPRESSION</scope>
        <scope>MUTAGENESIS OF LYS-72</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2004" name="J. Biol. Chem." volume="279" first="43008" last="43012">
            <title>A CDC6-like factor from the archaea Sulfolobus solfataricus promotes binding of the mini-chromosome maintenance complex to DNA.</title>
            <authorList>
                <person name="De Felice M."/>
                <person name="Esposito L."/>
                <person name="Pucci B."/>
                <person name="De Falco M."/>
                <person name="Rossi M."/>
                <person name="Pisani F.M."/>
            </authorList>
            <dbReference type="PubMed" id="15292191"/>
            <dbReference type="DOI" id="10.1074/jbc.M406693200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH MCM</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2006" name="Extremophiles" volume="10" first="61" last="70">
            <title>Biochemical characterization of two Cdc6/ORC1-like proteins from the crenarchaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="De Felice M."/>
                <person name="Esposito L."/>
                <person name="Rossi M."/>
                <person name="Pisani F.M."/>
            </authorList>
            <dbReference type="PubMed" id="16179962"/>
            <dbReference type="DOI" id="10.1007/s00792-005-0473-0"/>
        </citation>
        <scope>INTERACTION WITH CDC6-3</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2007" name="Biochem. Biophys. Res. Commun." volume="361" first="651" last="658">
            <title>Divergent functions of multiple eukaryote-like Orc1/Cdc6 proteins on modulating the loading of the MCM helicase onto the origins of the hyperthermophilic archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Jiang P.X."/>
                <person name="Wang J."/>
                <person name="Feng Y."/>
                <person name="He Z.G."/>
            </authorList>
            <dbReference type="PubMed" id="17673179"/>
            <dbReference type="DOI" id="10.1016/j.bbrc.2007.07.073"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH MCM</scope>
        <scope>DOMAIN</scope>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2007" name="Biochem. Biophys. Res. Commun." volume="363" first="63" last="70">
            <title>Three eukaryote-like Orc1/Cdc6 proteins functionally interact and mutually regulate their activities of binding to the replication origin in the hyperthermophilic archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Wang J."/>
                <person name="Jiang P.X."/>
                <person name="Feng H."/>
                <person name="Feng Y."/>
                <person name="He Z.G."/>
            </authorList>
            <dbReference type="PubMed" id="17825793"/>
            <dbReference type="DOI" id="10.1016/j.bbrc.2007.08.125"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH CDC6-3</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2009" name="Proc. Natl. Acad. Sci. U.S.A." volume="106" first="7792" last="7797">
            <title>Archaeal eukaryote-like Orc1/Cdc6 initiators physically interact with DNA polymerase B1 and regulate its functions.</title>
            <authorList>
                <person name="Zhang L."/>
                <person name="Zhang L."/>
                <person name="Liu Y."/>
                <person name="Yang S."/>
                <person name="Gao C."/>
                <person name="Gong H."/>
                <person name="Feng Y."/>
                <person name="He Z.G."/>
            </authorList>
            <dbReference type="PubMed" id="19416914"/>
            <dbReference type="DOI" id="10.1073/pnas.0813056106"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH POLB1</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 3 5 6 7">Involved in regulation of DNA replication. May play essential roles in origin recognition and cell cycle control of replication. Binds both single-stranded and double-stranded DNA (PubMed:12966100), with a preference for molecules that contain a bubble, a fork, or a tail (PubMed:15292191). Has a weak ATPase activity (PubMed:12966100). Stimulates the binding of the MCM helicase to the origin DNA (PubMed:17673179), but strongly inhibits ATPase and DNA helicase activities of MCM (PubMed:12966100). Also regulates the DNA polymerase and the nuclease activities of PolB1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 3 4 5 6 7">Monomer (PubMed:15292191). Interacts with Cdc6-3, MCM (PubMed:15292191, PubMed:17673179) and PolB1.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-15778726">
            <id>Q9UXF8</id>
        </interactant>
        <interactant intactId="EBI-15778666">
            <id>P26811</id>
            <label>dpo1</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>5</experiments>
    </comment>
    <comment type="domain">
        <text evidence="3 5">With a non-replication-origin bubble DNA, the C-terminal WH domain is not required for its stimulating effect on the MCM-loading activity (PubMed:15292191). With a forked origin-specific DNA substrate, the C-terminal WH domain is required to stimulate MCM-loading on DNA (PubMed:17673179).</text>
    </comment>
    <comment type="PTM">
        <text evidence="2">Autophosphorylated in vitro.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">Present with 2000-4000 molecules/cell at OD 600 nm 0.7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="2"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90226">
        <property type="entry name" value="E90226"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991361.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXF8"/>
    <dbReference type="SMR" id="Q9UXF8"/>
    <dbReference type="DIP" id="DIP-48852N"/>
    <dbReference type="FunCoup" id="Q9UXF8">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="IntAct" id="Q9UXF8">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0771"/>
    <dbReference type="PaxDb" id="273057-SSO0771"/>
    <dbReference type="EnsemblBacteria" id="AAK41068">
        <property type="protein sequence ID" value="AAK41068"/>
        <property type="gene ID" value="SSO0771"/>
    </dbReference>
    <dbReference type="GeneID" id="1455035"/>
    <dbReference type="KEGG" id="sso:SSO0771"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.771"/>
    <dbReference type="eggNOG" id="arCOG00467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025112_3_2_2"/>
    <dbReference type="InParanoid" id="Q9UXF8"/>
    <dbReference type="PhylomeDB" id="Q9UXF8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08768">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:001232">
        <property type="entry name" value="ORC1-type DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01407">
        <property type="entry name" value="ORC1_type_DNA_replic_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055237">
        <property type="entry name" value="Cdc6_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02928">
        <property type="entry name" value="orc1/cdc6 family replication initiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001623">
        <property type="entry name" value="PRK00411.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF31">
        <property type="entry name" value="ORC1-TYPE DNA REPLICATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22703">
        <property type="entry name" value="Cdc6_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151019" description="ORC1-type DNA replication protein 2">
        <location>
            <begin position="1"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="70"/>
            <end position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Loss of ATPase activity. Loss of autophosphorylation still inhibits helicase and ATPase of MCM." evidence="2">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12966100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="15292191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="16179962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="17673179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="17825793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="19416914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="8">
        <source>
            <dbReference type="PubMed" id="12966100"/>
        </source>
    </evidence>
    <sequence length="413" mass="47533" checksum="3CFFCFF405ACD01C" modified="2000-05-01" version="1">MVSAKDILSDSLRSSVLIIKHKDKLSPDYVPENLPHREEKIKELGFIFKDLLAGDAKDSERVVILGRTGTGKTATVRLFGKNFEDIAEREYGVKVKYVHINCYRHRTLYLISQEIANALKLPIPSRGLSAQEVFKMIHEYLDRRNIHLIVALDEFGHFLNTANTEEIYFLVRLYDEISAIIKRISYIFIVNESHSIYKLDRSIRDHIARRLIEFPPYKSMELYDILKYRVDEAFNDNAVDDEVLQFISNTYGYDKGGNGNARIAIETLSLAGEIAEKEGSPVVLLDHAKKANSTINPEIQEIIDSLSYLDLHQLILLKALIRALNKTKADEITMGTLEEEYISLSREFNEEPRRHTQVYEYLRKLKVIGIINTRQSGKGMRGRTTLVSLSLPLDKRLDDYIMQQIMVRLKSRA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-29" modified="2026-01-28" version="147" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXG1</accession>
    <name>MCM_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Minichromosome maintenance protein MCM</fullName>
            <ecNumber evidence="5 8 10">5.6.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">DNA 3'-5' helicase MCM</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Replicative DNA helicase MCM</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">MCM</name>
        <name type="ordered locus">SSO0774</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="12118" last="12127">
            <title>Physical and functional interaction between the mini-chromosome maintenance-like DNA helicase and the single-stranded DNA binding protein from the crenarchaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Carpentieri F."/>
                <person name="De Felice M."/>
                <person name="De Falco M."/>
                <person name="Rossi M."/>
                <person name="Pisani F.M."/>
            </authorList>
            <dbReference type="PubMed" id="11821426"/>
            <dbReference type="DOI" id="10.1074/jbc.M200091200"/>
        </citation>
        <scope>FUNCTION AS A DNA HELICASE</scope>
        <scope>FUNCTION AS AN ATPASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH SSB</scope>
        <scope>MUTAGENESIS OF LYS-346</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2003" name="J. Biol. Chem." volume="278" first="46424" last="46431">
            <title>Biochemical characterization of a CDC6-like protein from the crenarchaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="De Felice M."/>
                <person name="Esposito L."/>
                <person name="Pucci B."/>
                <person name="Carpentieri F."/>
                <person name="De Falco M."/>
                <person name="Rossi M."/>
                <person name="Pisani F.M."/>
            </authorList>
            <dbReference type="PubMed" id="12966100"/>
            <dbReference type="DOI" id="10.1074/jbc.M306075200"/>
        </citation>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH CDC6-2</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2004" name="J. Biol. Chem." volume="279" first="43008" last="43012">
            <title>A CDC6-like factor from the archaea Sulfolobus solfataricus promotes binding of the mini-chromosome maintenance complex to DNA.</title>
            <authorList>
                <person name="De Felice M."/>
                <person name="Esposito L."/>
                <person name="Pucci B."/>
                <person name="De Falco M."/>
                <person name="Rossi M."/>
                <person name="Pisani F.M."/>
            </authorList>
            <dbReference type="PubMed" id="15292191"/>
            <dbReference type="DOI" id="10.1074/jbc.M406693200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>INTERACTION WITH CDC6-2</scope>
        <scope>DOMAIN</scope>
        <scope>DNA-BINDING</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2005" name="Nat. Struct. Mol. Biol." volume="12" first="756" last="762">
            <title>Organization of the archaeal MCM complex on DNA and implications for the helicase mechanism.</title>
            <authorList>
                <person name="McGeoch A.T."/>
                <person name="Trakselis M.A."/>
                <person name="Laskey R.A."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="16116441"/>
            <dbReference type="DOI" id="10.1038/nsmb974"/>
        </citation>
        <scope>FUNCTION AS A DNA HELICASE</scope>
        <scope>FUNCTION AS AN ATPASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
        <scope>DOMAIN</scope>
        <scope>DNA-BINDING</scope>
        <scope>MUTAGENESIS OF 246-LYS-ARG-247 AND LYS-430</scope>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2006" name="EMBO Rep." volume="7" first="539" last="545">
            <title>GINS, a central nexus in the archaeal DNA replication fork.</title>
            <authorList>
                <person name="Marinsek N."/>
                <person name="Barry E.R."/>
                <person name="Makarova K.S."/>
                <person name="Dionne I."/>
                <person name="Koonin E.V."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="16485022"/>
            <dbReference type="DOI" id="10.1038/sj.embor.7400649"/>
        </citation>
        <scope>INTERACTION WITH GINS23</scope>
        <scope>OPERON STRUCTURE</scope>
        <scope>DOMAIN</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2007" name="Biochem. Biophys. Res. Commun." volume="361" first="651" last="658">
            <title>Divergent functions of multiple eukaryote-like Orc1/Cdc6 proteins on modulating the loading of the MCM helicase onto the origins of the hyperthermophilic archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Jiang P.X."/>
                <person name="Wang J."/>
                <person name="Feng Y."/>
                <person name="He Z.G."/>
            </authorList>
            <dbReference type="PubMed" id="17673179"/>
            <dbReference type="DOI" id="10.1016/j.bbrc.2007.07.073"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>INTERACTION WITH CDC6-1; CDC6-2 AND CDC6-3</scope>
        <scope>DOMAIN</scope>
        <scope>DNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="9">
        <citation type="journal article" date="2007" name="Mol. Cell" volume="28" first="304" last="314">
            <title>ATPase site architecture and helicase mechanism of an archaeal MCM.</title>
            <authorList>
                <person name="Moreau M.J."/>
                <person name="McGeoch A.T."/>
                <person name="Lowe A.R."/>
                <person name="Itzhaki L.S."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="17964268"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2007.08.013"/>
        </citation>
        <scope>FUNCTION AS A DNA HELICASE</scope>
        <scope>FUNCTION AS AN ATPASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF PHE-318; ARG-331; LYS-346; ARG-359; LYS-366; THR-374; ASP-404; 423-GLN-GLN-424; ASN-448; ARG-473; ASP-488 AND ARG-560</scope>
    </reference>
    <reference evidence="20" key="10">
        <citation type="journal article" date="2008" name="Nucleic Acids Res." volume="36" first="3235" last="3243">
            <title>Structural analysis of the Sulfolobus solfataricus MCM protein N-terminal domain.</title>
            <authorList>
                <person name="Liu W."/>
                <person name="Pucci B."/>
                <person name="Rossi M."/>
                <person name="Pisani F.M."/>
                <person name="Ladenstein R."/>
            </authorList>
            <dbReference type="PubMed" id="18417534"/>
            <dbReference type="DOI" id="10.1093/nar/gkn183"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 1-268 IN COMPLEX WITH ZN(2+)</scope>
        <scope>SUBUNIT</scope>
        <scope>DOMAIN</scope>
        <scope>DNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="21" key="11">
        <citation type="journal article" date="2008" name="Proc. Natl. Acad. Sci. U.S.A." volume="105" first="20191" last="20196">
            <title>Crystal structure of a near-full-length archaeal MCM: functional insights for an AAA+ hexameric helicase.</title>
            <authorList>
                <person name="Brewster A.S."/>
                <person name="Wang G."/>
                <person name="Yu X."/>
                <person name="Greenleaf W.B."/>
                <person name="Carazo J.M."/>
                <person name="Tjajadia M."/>
                <person name="Klein M.G."/>
                <person name="Chen X.S."/>
            </authorList>
            <dbReference type="PubMed" id="19073923"/>
            <dbReference type="DOI" id="10.1073/pnas.0808037105"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (4.35 ANGSTROMS) OF 7-601</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF 189-LEU--ASP-191; 202-GLU--VAL-204; 326-GLU--ARG-329; 416-ALA--ALA-420; 550-THR--PRO-554 AND 555-ILE--ASP-557</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="12">
        <citation type="journal article" date="2009" name="Proc. Natl. Acad. Sci. U.S.A." volume="106" first="6023" last="6023">
            <authorList>
                <person name="Brewster A.S."/>
                <person name="Wang G."/>
                <person name="Yu X."/>
                <person name="Greenleaf W.B."/>
                <person name="Carazo J.M."/>
                <person name="Tjajadi M."/>
                <person name="Klein M.G."/>
                <person name="Chen X.S."/>
            </authorList>
            <dbReference type="DOI" id="10.1073/pnas.0901568106"/>
        </citation>
        <scope>ERRATUM OF PUBMED:19073923</scope>
    </reference>
    <reference evidence="23" key="13">
        <citation type="journal article" date="2019" name="Nat. Commun." volume="10" first="3117" last="3117">
            <title>DNA translocation mechanism of the MCM complex and implications for replication initiation.</title>
            <authorList>
                <person name="Meagher M."/>
                <person name="Epling L.B."/>
                <person name="Enemark E.J."/>
            </authorList>
            <dbReference type="PubMed" id="31308367"/>
            <dbReference type="DOI" id="10.1038/s41467-019-11074-3"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS) OF 2-612 IN COMPLEX WITH SSDNA; ATP ANALOGS; MG(2+) AND ZN(2+)</scope>
        <scope>FUNCTION AS A HELICASE</scope>
        <scope>POSSIBLE REACTION MECHANISM</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>DOMAIN</scope>
        <scope>DNA-BINDING</scope>
        <scope>MUTAGENESIS OF 266-GLN--ILE-274; THR-369; TYR-386 AND LYS-430</scope>
    </reference>
    <reference evidence="24 25 26" key="14">
        <citation type="journal article" date="2022" name="Int. J. Mol. Sci." volume="23" first="14678" last="14678">
            <title>Two Distinct Modes of DNA Binding by an MCM Helicase Enable DNA Translocation.</title>
            <authorList>
                <person name="Meagher M."/>
                <person name="Myasnikov A."/>
                <person name="Enemark E.J."/>
            </authorList>
            <dbReference type="PubMed" id="36499022"/>
            <dbReference type="DOI" id="10.3390/ijms232314678"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.34 ANGSTROMS) OF 2-612 IN COMPLEX WITH SSDNA; ATP ANALOGS; MG(2+) AND ZN(2+)</scope>
        <scope>REACTION MECHANISM</scope>
        <scope>SUBUNIT</scope>
        <scope>DOMAIN</scope>
        <scope>DNA-BINDING</scope>
    </reference>
    <comment type="function">
        <text evidence="2 4 5 7 8 11 12 16 19">The 3'-5' replicative DNA helicase. The homohexamer forms a spiral staircase around ssDNA (PubMed:31308367) with a nucleotide-binding site formed at each interface (PubMed:17964268, PubMed:31308367). This ring pulls the DNA strand of through its central pore in a hand-over-hand mechanism, where the subunit at the bottom of the staircase dissociates from DNA and re-binds DNA one step above the staircase after ATP hydrolysis (PubMed:31308367, PubMed:36499022). A complete cycle translocates 12 nucleotides of ssDNA through the central channel with hydrolysis of 6 ATP molecules; the complex probably tolerates multiple catalytically inactive subunits (Probable) (PubMed:17964268, PubMed:31308367). Has (d)ATP-dependent 3'-5' DNA helicase and ATPase activity; other nucleotides can be used for DNA helicase activity (PubMed:11821426, PubMed:12966100). ATPase activity is stimulated by DNA (PubMed:16116441). Helicase melts 5'-tailed oligonucleotides on circular single-stranded (ss)DNA and is stimulated by the SSB protein (single-stranded DNA binding protein) (PubMed:11821426, PubMed:12966100). Another group shows helicase activity on 3'-tailed linear substrates and Y-shaped DNA (PubMed:16116441, PubMed:17964268). Binds both single-stranded and double-stranded DNA, with a preference for molecules that contain a bubble, a fork, or a tail (PubMed:15292191, PubMed:17673179). The active ATPase sites in the MCM ring are formed through the interaction surfaces of two neighboring subunits; a critical, conserved arginine finger motif (Arg-473) is provided in trans relative to the ATP-binding site of the Walker A and B motifs of the adjacent subunit (PubMed:17964268, PubMed:31308367).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 10 19">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3 5 8 10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 11 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 11 12 23">Binds 1 Zn(2+) per monomer.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2 3 4 7">Cdc6-2 inhibits helicase and ATPase activity (PubMed:12966100). Binding of MCM to bubble or origin-specific forked DNA is stimulated by Cdc6-2 (PubMed:15292191, PubMed:17673179). Cdc6-1 or Cdc6-3 inhibit MCM binding to origin-specific forked DNA (PubMed:17673179). The ATPase activity is not stimulated in the presence of DNA molecules (PubMed:11821426). Unwinding activity is stimulated by single-stranded DNA binding protein (SSB) (PubMed:11821426).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3 4 5 6 8 10 11 17">Homohexamers (PubMed:11821426, PubMed:16116441, PubMed:17964268, PubMed:18417534, PubMed:19073923, PubMed:31308367). Forms a complex with SSB (PubMed:11821426). Interacts with Cdc6-2 (PubMed:12966100, PubMed:15292191). Interacts with Gins23 (and probably the whole CG complex) in all growth phases (PubMed:16485022). Interacts with Cdc6-1, Cdc6-2 and Cdc6-3 (PubMed:17673179).</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-8081298">
            <id>Q9UXG1</id>
        </interactant>
        <interactant intactId="EBI-8081298">
            <id>Q9UXG1</id>
            <label>MCM</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>9</experiments>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-8081298">
            <id>Q9UXG1</id>
        </interactant>
        <interactant intactId="EBI-8081318">
            <id>Q9UXG0</id>
            <label>ORF-c40_009</label>
        </interactant>
        <organismsDiffer>true</organismsDiffer>
        <experiments>5</experiments>
    </comment>
    <comment type="induction">
        <text evidence="6">Expressed during early, mid- and late-logarithmic growth (at protein level). Encoded in the gins23-MCM operon.</text>
    </comment>
    <comment type="domain">
        <text evidence="4 5 6 8 9 10 11 12 18">The homohexamer forms around a central hole with 2 tiers; the N-terminal tier (residues 1-275) and the C-terminal ATPase tier (about residues 280-612); ssDNA is bound by the C-terminal tier (PubMed:31308367, PubMed:36499022). Both the N-terminus (residues 1-265) and AAA+ motor domain (residues 306-601) contribute to binding of Y-DNA (PubMed:16116441, PubMed:19073923). The N-terminus (residues 1-268) is not required to bind Cdc6-2 (PubMed:15292191). The N-terminus (residues 1-266) binds Gins23 (PubMed:16485022). The WH domain has not been crystallized, but may be flexible (Probable) (PubMed:19073923). The N-terminus binds very poorly to blunt double-stranded (ds)DNA, while whole protein and the AAA+ motor domain bind cooperatively to dsDNA (PubMed:18417534). The hexamer is oriented with the motor domain facing the dsDNA to be unwound (PubMed:16116441). The isolated N-terminus crystallizes as a hexamer with a central pore large enough for ss- but not dsDNA (PubMed:17964268). The Zn(2+) ligand is close to the central pore (PubMed:17964268, PubMed:18417534, PubMed:31308367, PubMed:36499022).</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the MCM family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAB57529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="AAK41071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="H90226"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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        <property type="method" value="NMR"/>
        <property type="chains" value="A=605-686"/>
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        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B/C=1-268"/>
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    <dbReference type="PDB" id="3F9V">
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        <property type="resolution" value="4.35 A"/>
        <property type="chains" value="A=7-601"/>
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        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B=2-269"/>
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        <property type="resolution" value="3.15 A"/>
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    </dbReference>
    <dbReference type="PDB" id="6WNZ">
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        <property type="resolution" value="2.00 A"/>
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        <property type="method" value="EM"/>
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        <property type="resolution" value="2.59 A"/>
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    </dbReference>
    <dbReference type="PDBsum" id="2M45"/>
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    <dbReference type="AlphaFoldDB" id="Q9UXG1"/>
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    <dbReference type="EMDB" id="EMD-27974"/>
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    <dbReference type="EMDB" id="EMD-27980"/>
    <dbReference type="EMDB" id="EMD-27981"/>
    <dbReference type="SMR" id="Q9UXG1"/>
    <dbReference type="DIP" id="DIP-42884N"/>
    <dbReference type="FunCoup" id="Q9UXG1">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="IntAct" id="Q9UXG1">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="MINT" id="Q9UXG1"/>
    <dbReference type="STRING" id="273057.SSO0774"/>
    <dbReference type="PaxDb" id="273057-SSO0774"/>
    <dbReference type="EnsemblBacteria" id="AAK41071">
        <property type="protein sequence ID" value="AAK41071"/>
        <property type="gene ID" value="SSO0774"/>
    </dbReference>
    <dbReference type="GeneID" id="1455038"/>
    <dbReference type="GeneID" id="44129780"/>
    <dbReference type="KEGG" id="sso:SSO0774"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.774"/>
    <dbReference type="eggNOG" id="arCOG00439">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_6_0_2"/>
    <dbReference type="InParanoid" id="Q9UXG1"/>
    <dbReference type="PhylomeDB" id="Q9UXG1"/>
    <dbReference type="BRENDA" id="3.6.4.12">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q9UXG1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000353"/>
        <property type="project" value="IntAct"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17761">
        <property type="entry name" value="MCM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000630">
        <property type="entry name" value="DNA replication licensing factor (Mcm)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000826">
        <property type="entry name" value="Replicative DNA helicase Mcm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048907">
        <property type="entry name" value="WHD_MCM_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040949">
        <property type="entry name" value="minchrom_main_MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21100">
        <property type="entry name" value="WHD_MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000194128" description="Minichromosome maintenance protein MCM">
        <location>
            <begin position="1"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="1 10">
        <location>
            <begin position="274"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminus" evidence="10">
        <location>
            <begin position="1"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="WH domain" evidence="18">
        <location>
            <begin position="622"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Walker A" evidence="1">
        <location>
            <begin position="340"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Walker B" evidence="1">
        <location>
            <begin position="404"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20 22 23">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20 22 23">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20 22 23">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20 22 23">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 19 23">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 19 23">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 19 23">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 19 23">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 19 23">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12 23">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 19 23">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12 23">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>ssDNA</name>
            <dbReference type="ChEBI" id="CHEBI:9160"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12 23">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>ssDNA</name>
            <dbReference type="ChEBI" id="CHEBI:9160"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 23">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>ssDNA</name>
            <dbReference type="ChEBI" id="CHEBI:9160"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 19 23">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12 23">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>ssDNA</name>
            <dbReference type="ChEBI" id="CHEBI:9160"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12 23">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>ssDNA</name>
            <dbReference type="ChEBI" id="CHEBI:9160"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 19 23">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 19 23">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 19 23">
        <location>
            <position position="560"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 19 23">
        <location>
            <position position="563"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Arginine finger" evidence="1 16">
        <location>
            <position position="473"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Predominantly monomeric, loss of helicase activity." evidence="10">
        <original>LID</original>
        <variation>DIR</variation>
        <location>
            <begin position="189"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of helicase activity, remains a hexamer, still has ATPase activity, binds DNA." evidence="10">
        <original>EEV</original>
        <variation>GGG</variation>
        <location>
            <begin position="202"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Fork DNA no longer stimulates ATPase, decreased helicase activity and DNA binding. Loss of helicase activity and DNA binding; when associated with A-430." evidence="5">
        <original>KR</original>
        <variation>AA</variation>
        <location>
            <begin position="246"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Wild-type helicase activity, stronger helicase cooperativity; when associated with C-terminal deletion." evidence="11">
        <original>QKVLDEVII</original>
        <variation>GGSGGS</variation>
        <location>
            <begin position="266"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect on helicase and ATPase activity." evidence="8">
        <original>F</original>
        <variation>A</variation>
        <location>
            <position position="318"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Impairs helicase activity, remains a hexamer." evidence="10">
        <original>EDTR</original>
        <variation>AATA</variation>
        <location>
            <begin position="326"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of helicase and ATPase activity." evidence="8">
        <original>R</original>
        <variation>A</variation>
        <location>
            <position position="331"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Sharp decrease in ATPase activity. Almost devoid of helicase activity." evidence="2 8">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="346"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of helicase, reduction of ATPase activity." evidence="8">
        <original>R</original>
        <variation>A</variation>
        <location>
            <position position="359"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of helicase, reduction of ATPase activity." evidence="8">
        <original>K</original>
        <variation>E</variation>
        <location>
            <position position="366"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Aggregates. Reduced strand displacement; when associated with 265-G--S-275." evidence="11">
        <original>T</original>
        <variation>A</variation>
        <location>
            <position position="369"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduction of helicase, increase of ATPase activity." evidence="8">
        <original>T</original>
        <variation>E</variation>
        <location>
            <position position="374"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No significant change in helicase activity." evidence="11">
        <original>Y</original>
        <variation>A</variation>
        <location>
            <position position="386"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of helicase and ATPase activity." evidence="8">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="404"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Predominantly monomeric, loss of helicase activity." evidence="10">
        <original>AIHEA</original>
        <variation>RIHER</variation>
        <location>
            <begin position="416"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of helicase and ATPase activity." evidence="8">
        <original>QQ</original>
        <variation>AA</variation>
        <location>
            <begin position="423"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Fork DNA no longer stimulates ATPase, decreased DNA binding, loss of helicase activity. Loss of helicase activity and DNA binding; when associated with 246-A-A-247." evidence="5 11">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="430"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of helicase and ATPase activity." evidence="8">
        <original>N</original>
        <variation>A</variation>
        <location>
            <position position="448"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of helicase and ATPase activity." evidence="8">
        <original>R</original>
        <variation>A</variation>
        <location>
            <position position="473"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect on helicase and reduction of ATPase activity, forms dimers or trimers." evidence="8">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="488"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Predominantly monomeric, loss of helicase activity, still has ATPase activity, binds DNA." evidence="10">
        <original>TPDSP</original>
        <variation>GGGGG</variation>
        <location>
            <begin position="550"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Exclusively monomeric, loss of helicase activity, ATPase activity and DNA binding." evidence="10">
        <original>ILI</original>
        <variation>DSD</variation>
        <location>
            <begin position="555"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of helicase and ATPase activity." evidence="8">
        <original>R</original>
        <variation>A</variation>
        <location>
            <position position="560"/>
        </location>
    </feature>
    <feature type="helix" evidence="29">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="strand" evidence="28">
        <location>
            <begin position="24"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="helix" evidence="29">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="strand" evidence="29">
        <location>
            <begin position="44"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="helix" evidence="29">
        <location>
            <begin position="49"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="helix" evidence="29">
        <location>
            <begin position="57"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="helix" evidence="29">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="helix" evidence="29">
        <location>
            <begin position="89"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="29">
        <location>
            <begin position="97"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="helix" evidence="29">
        <location>
            <begin position="109"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="helix" evidence="29">
        <location>
            <begin position="114"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="strand" evidence="29">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="strand" evidence="29">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="turn" evidence="29">
        <location>
            <begin position="147"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="strand" evidence="29">
        <location>
            <begin position="152"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="turn" evidence="29">
        <location>
            <begin position="172"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="strand" evidence="29">
        <location>
            <begin position="179"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="helix" evidence="28">
        <location>
            <begin position="184"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="strand" evidence="29">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="helix" evidence="29">
        <location>
            <begin position="201"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="strand" evidence="29">
        <location>
            <begin position="212"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="helix" evidence="29">
        <location>
            <begin position="219"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="strand" evidence="29">
        <location>
            <begin position="230"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="strand" evidence="29">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="helix" evidence="27">
        <location>
            <begin position="612"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="helix" evidence="27">
        <location>
            <begin position="621"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="strand" evidence="27">
        <location>
            <begin position="640"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="helix" evidence="27">
        <location>
            <begin position="645"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="helix" evidence="27">
        <location>
            <begin position="659"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="strand" evidence="27">
        <location>
            <begin position="674"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="strand" evidence="27">
        <location>
            <begin position="682"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11821426"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PubMed" id="12966100"/>
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    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="15292191"/>
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    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="16116441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="16485022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="17673179"/>
        </source>
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    <evidence type="ECO:0000269" key="8">
        <source>
            <dbReference type="PubMed" id="17964268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="9">
        <source>
            <dbReference type="PubMed" id="18417534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="10">
        <source>
            <dbReference type="PubMed" id="19073923"/>
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        <source>
            <dbReference type="PubMed" id="31308367"/>
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    </evidence>
    <evidence type="ECO:0000269" key="12">
        <source>
            <dbReference type="PubMed" id="36499022"/>
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        <source>
            <dbReference type="PubMed" id="10701121"/>
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    </evidence>
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        <source>
            <dbReference type="PubMed" id="11821426"/>
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    <evidence type="ECO:0000305" key="16">
        <source>
            <dbReference type="PubMed" id="17964268"/>
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    </evidence>
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        <source>
            <dbReference type="PubMed" id="18417534"/>
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        <source>
            <dbReference type="PubMed" id="19073923"/>
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            <dbReference type="PubMed" id="31308367"/>
        </source>
    </evidence>
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    </evidence>
    <evidence type="ECO:0007744" key="21">
        <source>
            <dbReference type="PDB" id="3F9V"/>
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    <evidence type="ECO:0007744" key="22">
        <source>
            <dbReference type="PDB" id="4FDG"/>
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    </evidence>
    <evidence type="ECO:0007744" key="23">
        <source>
            <dbReference type="PDB" id="6MII"/>
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    </evidence>
    <evidence type="ECO:0007744" key="24">
        <source>
            <dbReference type="PDB" id="8EAF"/>
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        <source>
            <dbReference type="PDB" id="8EAG"/>
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    <evidence type="ECO:0007744" key="26">
        <source>
            <dbReference type="PDB" id="8EAH"/>
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    </evidence>
    <evidence type="ECO:0007829" key="27">
        <source>
            <dbReference type="PDB" id="2M45"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="28">
        <source>
            <dbReference type="PDB" id="2VL6"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="29">
        <source>
            <dbReference type="PDB" id="6WNZ"/>
        </source>
    </evidence>
    <sequence length="686" mass="77428" checksum="C3ECD380F272E77E" modified="2000-05-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1999-07-15" modified="2024-07-24" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>P81551</accession>
    <name>S7C3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Repressor-like protein SSo7c3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO5984</name>
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    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="FEBS Lett." volume="432" first="141" last="144">
            <title>Isolation and structure of repressor-like proteins from the archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Oppermann U.C.T."/>
                <person name="Knapp S."/>
                <person name="Bonetto V."/>
                <person name="Ladenstein R."/>
                <person name="Joernvall H."/>
            </authorList>
            <dbReference type="PubMed" id="9720912"/>
            <dbReference type="DOI" id="10.1016/S0014-5793(98)00848-5"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-52</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0003677">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="SpoVT-AbrB_dom"/>
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        <property type="entry name" value="lp_hng_hel_AbrB"/>
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    <keyword id="KW-0903">Direct protein sequencing</keyword>
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        <location>
            <position position="1"/>
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            <begin position="2"/>
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            <begin position="4"/>
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    <feature type="sequence conflict" description="In Ref. 1." evidence="3" ref="1">
        <original>G</original>
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            <position position="41"/>
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    <feature type="sequence conflict" description="In Ref. 1." evidence="3" ref="1">
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            <begin position="50"/>
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    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="PubMed" id="9720912"/>
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    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="52" mass="5854" checksum="86C62E559B5385B5" modified="2007-01-23" version="2">MPVEEVVKVSRNYQVTIPAKVRQKFPVKEGDLVKVIYDENGGVVKIQILDSP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2026-01-28" version="141" xmlns="https://uniprot.org/uniprot">
    <accession>P95895</accession>
    <name>TDXH_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peroxiredoxin</fullName>
            <ecNumber evidence="1">1.11.1.24</ecNumber>
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        <alternativeName>
            <fullName evidence="1">Thioredoxin-dependent peroxiredoxin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2121</name>
        <name type="ORF">C02015</name>
        <name type="ORF">C02_016</name>
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        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodecamer. Pentamer of dimers that assemble into a ring structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this 1-Cys peroxiredoxin, no C(R) is present and C(P) instead forms a disulfide with a cysteine from another protein or with a small thiol molecule.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="1"/>
    <dbReference type="EMBL" id="Y08256">
        <property type="protein sequence ID" value="CAA69447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74033">
        <property type="entry name" value="S74033"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990153.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95895"/>
    <dbReference type="SMR" id="P95895"/>
    <dbReference type="FunCoup" id="P95895">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2121"/>
    <dbReference type="PaxDb" id="273057-SSO2121"/>
    <dbReference type="EnsemblBacteria" id="AAK42300">
        <property type="protein sequence ID" value="AAK42300"/>
        <property type="gene ID" value="SSO2121"/>
    </dbReference>
    <dbReference type="GeneID" id="1453627"/>
    <dbReference type="KEGG" id="sso:SSO2121"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2211"/>
    <dbReference type="eggNOG" id="arCOG00312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_4_4_2"/>
    <dbReference type="InParanoid" id="P95895"/>
    <dbReference type="PhylomeDB" id="P95895"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140824">
        <property type="term" value="F:thioredoxin-dependent peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1020.10:FF:000002">
        <property type="entry name" value="Peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000011">
        <property type="entry name" value="Peroxiredoxin PRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1020.10">
        <property type="entry name" value="Antioxidant, Horf6, Chain A, domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00401">
        <property type="entry name" value="Peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022915">
        <property type="entry name" value="Peroxiredoxin_TDXH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045020">
        <property type="entry name" value="PRX_1cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009668">
        <property type="entry name" value="PRK13189.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009670">
        <property type="entry name" value="PRK13191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF121">
        <property type="entry name" value="ALKYL HYDROPEROXIDE REDUCTASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0049">Antioxidant</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0575">Peroxidase</keyword>
    <keyword id="KW-0676">Redox-active center</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135169" description="Peroxiredoxin">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="7"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="1">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00401"/>
        </source>
    </evidence>
    <sequence length="215" mass="24745" checksum="5210477EF4C9607B" modified="1997-05-01" version="1">MSEERIPLIGERFPEMEVITTQGRIKLPDDYKGRWFVLFSHPGDFTPVCTTEFYSFAKKYEEFKKLNTELIGLSVDSNISHIEWIMWIEKNLKVEIPFPIIADPMGNVAKRLGMIHAQSSTATVRAVFVVDDKGVVRLILYYPMEIGRNIDEILRAIRALQLVDKAGVVTPANWPNNELIGDKVINPAPRTIKDAKMRLGQPFDWWFTYKEVKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX43</accession>
    <name>Q7LX43_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dehydrogenase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2162</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74069">
        <property type="entry name" value="S74069"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923795.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX43"/>
    <dbReference type="STRING" id="273057.SSO2162"/>
    <dbReference type="PaxDb" id="273057-SSO2162"/>
    <dbReference type="EnsemblBacteria" id="AAK42338">
        <property type="protein sequence ID" value="AAK42338"/>
        <property type="gene ID" value="SSO2162"/>
    </dbReference>
    <dbReference type="GeneID" id="1453665"/>
    <dbReference type="KEGG" id="sso:SSO2162"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2255"/>
    <dbReference type="eggNOG" id="arCOG00340">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_301816_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX43"/>
    <dbReference type="PhylomeDB" id="Q7LX43"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF118">
        <property type="entry name" value="ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE (PRECURSOR)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="4">
        <location>
            <begin position="42"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="506"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="1000" mass="110915" checksum="E80AD9A1D3F5AF1A" modified="2011-05-31" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX67</accession>
    <name>Q7LX67_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator homolog (Lrp/AsnC family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2131</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74043">
        <property type="entry name" value="S74043"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX67"/>
    <dbReference type="SMR" id="Q7LX67"/>
    <dbReference type="STRING" id="273057.SSO2131"/>
    <dbReference type="PaxDb" id="273057-SSO2131"/>
    <dbReference type="ABCD" id="Q7LX67">
        <property type="antibodies" value="3 sequenced antibodies"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK42310">
        <property type="protein sequence ID" value="AAK42310"/>
        <property type="gene ID" value="SSO2131"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2131"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2223"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_4_2"/>
    <dbReference type="InParanoid" id="Q7LX67"/>
    <dbReference type="PhylomeDB" id="Q7LX67"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="5">
        <location>
            <begin position="9"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="155" mass="17702" checksum="7DF242A9A69A2396" modified="2011-05-31" version="1">MMGVNIIRLDDTDEKILNILRYNAKKSLKELSDELGIPISTVRYRIKRLEDAQIIRGYVALIDRVNLGLNVSLVMEIETVPYSIKKVAQDLGEIPEVVRIYGLDNGPRLHVHMIFKDDASAHQFIANKLYNIKGIKTVSISRIIERYKIDPSVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-06-18" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXG4</accession>
    <name>Q7LXG4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="3 7">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ugd</name>
        <name evidence="12" type="ordered locus">SSO0810</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>UDP-alpha-D-glucose + 2 NAD(+) + H2O = UDP-alpha-D-glucuronate + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="3 7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90231">
        <property type="entry name" value="F90231"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923104.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXG4"/>
    <dbReference type="FunCoup" id="Q7LXG4">
        <property type="interactions" value="254"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0810"/>
    <dbReference type="PaxDb" id="273057-SSO0810"/>
    <dbReference type="EnsemblBacteria" id="AAK41109">
        <property type="protein sequence ID" value="AAK41109"/>
        <property type="gene ID" value="SSO0810"/>
    </dbReference>
    <dbReference type="GeneID" id="1455076"/>
    <dbReference type="KEGG" id="sso:SSO0810"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.823"/>
    <dbReference type="eggNOG" id="arCOG00253">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_1_2_2"/>
    <dbReference type="InParanoid" id="Q7LXG4"/>
    <dbReference type="PhylomeDB" id="Q7LXG4"/>
    <dbReference type="UniPathway" id="UPA00038">
        <property type="reaction ID" value="UER00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006065">
        <property type="term" value="P:UDP-glucuronate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028357">
        <property type="entry name" value="UDPglc_DH_bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43750">
        <property type="entry name" value="UDP-GLUCOSE 6-DEHYDROGENASE TUAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43750:SF3">
        <property type="entry name" value="UDP-GLUCOSE 6-DEHYDROGENASE TUAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500134">
        <property type="entry name" value="UDPglc_DH_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="UDP-glucose/GDP-mannose dehydrogenase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0520">NAD</keyword>
    <keyword evidence="4 7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDP-glucose/GDP-mannose dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="302"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="140"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="238"/>
            <end position="242"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK41109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="409" mass="44837" checksum="22EAB1AB796809D6" modified="2011-05-31" version="1">MRIGVVGLGVVGLVTGAVLADQGHEVVGVDIDQNKVKGLQCNRSPIYEPGLDELLLKNKNRFLFTTDYSALKDVDIVFITVATPTVEGKNYIGYVLDAAKTMKPYLKRDAIVVVKSTVVPGTSRKVKQIVEREVVANPEFLKEGSAVIDTIKPDRIVIGSDSKAAGDVIENLWSFTKTVVLRTSIEEAEMIKYAANSFLATKISFINEIANLCEVIPNCDVNTIAKAIGLDKRIAPYFLNAGLGYGGSCFPKDTLAFASFARELGEELKIVEAAIKVNEERPRRAVKIMEELLGELKGKTICILGIAFKPNTDDTRESVALKIAKLLVDIGANVIAYDPKAKTSLVEMVSSKEECVKRSDGVIIATEWDEFRGIEHMLKDKAVLDGRRVLDPNKMSRSKYRAIGLSKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXG7</accession>
    <name>Q7LXG7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polyketide cyclase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0807</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90231">
        <property type="entry name" value="C90231"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923101.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXG7"/>
    <dbReference type="STRING" id="273057.SSO0807"/>
    <dbReference type="PaxDb" id="273057-SSO0807"/>
    <dbReference type="EnsemblBacteria" id="AAK41106">
        <property type="protein sequence ID" value="AAK41106"/>
        <property type="gene ID" value="SSO0807"/>
    </dbReference>
    <dbReference type="GeneID" id="1455073"/>
    <dbReference type="KEGG" id="sso:SSO0807"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.817"/>
    <dbReference type="eggNOG" id="arCOG07227">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1248332_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXG7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07812">
        <property type="entry name" value="SRPBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="25542" checksum="903621A03C920B84" modified="2011-05-31" version="1">MEKAYDFEVEAPPEVVRDYLMNPENLMKYIPHFKDLKRVDDGWELYVSWLFTIKLKVKRVFTNTDIIYLVEKSEGLIKIKSSLRFTIFPSKGGITIVRLVFFYDGPFESIVKGQADAFYRKGKEVFKTEMTKEKRIQEEQNGVKGEVSIYEMKTIFSGKINKDELEKYLEIAMVKSVNSTVVVILSDERNIIEIKFKGGELVAQKGDLDSLSSPITILIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXG8</accession>
    <name>Q7LXG8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0805</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90231">
        <property type="entry name" value="A90231"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923099.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXG8"/>
    <dbReference type="PaxDb" id="273057-SSO0805"/>
    <dbReference type="EnsemblBacteria" id="AAK41104">
        <property type="protein sequence ID" value="AAK41104"/>
        <property type="gene ID" value="SSO0805"/>
    </dbReference>
    <dbReference type="GeneID" id="1455071"/>
    <dbReference type="KEGG" id="sso:SSO0805"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.815"/>
    <dbReference type="HOGENOM" id="CLU_1891531_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXG8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="134" mass="14857" checksum="E0AE325F5A62BC62" modified="2011-05-31" version="1">MTGQKNSESLVGELVNQINELYLSNPNQVLMLAKQAGIENPTFLATPPTKPSGELSFAKLSNIAEIVTKLENYGLPSLLRQYFMNVVKNPVVLNNVVETYYKIAKIIPKEVENEKPLVIENEGFASKSIPPVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXM2</accession>
    <name>Q7LXM2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90216">
        <property type="entry name" value="H90216"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXM2"/>
    <dbReference type="STRING" id="273057.SSO0691"/>
    <dbReference type="PaxDb" id="273057-SSO0691"/>
    <dbReference type="DNASU" id="1454958"/>
    <dbReference type="EnsemblBacteria" id="AAK40991">
        <property type="protein sequence ID" value="AAK40991"/>
        <property type="gene ID" value="SSO0691"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0691"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.690"/>
    <dbReference type="eggNOG" id="arCOG05930">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1040590_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXM2"/>
    <dbReference type="PhylomeDB" id="Q7LXM2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049688">
        <property type="entry name" value="CedA_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041796">
        <property type="entry name" value="Ced_CedA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="288" mass="31480" checksum="4348F30B1775A3D6" modified="2011-05-31" version="1">MDCIRNNSVLINNLSSFTSGIMSSGYSPFYILYIAMNIATLTYAVGTLFYGLPIPIYGLKKWGPRMMSDAIYAAVWVNIYGIIIFAIGQIQSLLGVDWSSFFSSILQLQANMFSALIQVKSLYYIITTEKISMALALLADPVLQFSSFITDIIFLLQFFIDLGEFIQQSYMILIAIGILLLSLPFRMGKGVGGTLISSAIIFYIGLPYLPIFMQEMSSITLSQIGSQLSTITDVNTLVETIAGVVPELVIVFIIIPMLYLSILAGISLGLGNAIGGSSGRVPFPLDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXS0</accession>
    <name>Q7LXS0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SpoVT-AbrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0618</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90209">
        <property type="entry name" value="B90209"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991151.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXS0"/>
    <dbReference type="STRING" id="273057.SSO0618"/>
    <dbReference type="PaxDb" id="273057-SSO0618"/>
    <dbReference type="EnsemblBacteria" id="AAK40929">
        <property type="protein sequence ID" value="AAK40929"/>
        <property type="gene ID" value="SSO0618"/>
    </dbReference>
    <dbReference type="GeneID" id="1454896"/>
    <dbReference type="KEGG" id="sso:SSO0618"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.626"/>
    <dbReference type="eggNOG" id="arCOG00318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069302_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LXS0"/>
    <dbReference type="PhylomeDB" id="Q7LXS0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF2">
        <property type="entry name" value="PHOU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="11"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="336" mass="37732" checksum="004F445833977AEE" modified="2011-05-31" version="1">MSKSITRKIQLTGGSTYIVSLPKEWIKALSLSAGDEVEIYQDTDMKLLIVPKSANTTDQKEIKKIIHCDNVTSEAIIREFIAYYIAGFSSVSITCPRMSSSTRAYIKDVVRRRLLGAEVIEEDVSTISIQFLVDEKELSIKKAISRAFTISYNMLRDSLEAISKADIELAKEISGRDDEVDRFFFYIARQLTISVKAINVLDSEGYNLTQMVDIYSVAKTIERVGDHANRIASLVEDVSKFSNKEDLVNLGLTILESYKSAMNAFLNEKKDIAHSLISNIEIFEKLRELQEIAIKSNDNPKIITSTSMTVESLRRVARYSADIAEATIDILAKSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXS6</accession>
    <name>Q7LXS6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0606</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90207">
        <property type="entry name" value="F90207"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991131.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXS6"/>
    <dbReference type="STRING" id="273057.SSO0606"/>
    <dbReference type="PaxDb" id="273057-SSO0606"/>
    <dbReference type="EnsemblBacteria" id="AAK40917">
        <property type="protein sequence ID" value="AAK40917"/>
        <property type="gene ID" value="SSO0606"/>
    </dbReference>
    <dbReference type="GeneID" id="1454884"/>
    <dbReference type="KEGG" id="sso:SSO0606"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.614"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_4_2"/>
    <dbReference type="InParanoid" id="Q7LXS6"/>
    <dbReference type="PhylomeDB" id="Q7LXS6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="5">
        <location>
            <begin position="11"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="157" mass="17975" checksum="EC93983CE1FD0FDB" modified="2011-05-31" version="1">MSDSKKRTVDLDAIDRRLLIELTRDARTSLRRLAEEMNVSPATLHNRMTRMVQEGMIKSFVALLDYSKLGFALTGIIMAKVDGKHLVEFEKEIANADNVTAVYDVVGEYDVVIIAKFRSVEELDSFLKQLLKNPKIERTYTSIVLNVVKEDPRIRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXY1</accession>
    <name>Q7LXY1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ParB-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0115</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75374">
        <property type="entry name" value="S75374"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXY1"/>
    <dbReference type="STRING" id="273057.SSO0115"/>
    <dbReference type="PaxDb" id="273057-SSO0115"/>
    <dbReference type="EnsemblBacteria" id="AAK40469">
        <property type="protein sequence ID" value="AAK40469"/>
        <property type="gene ID" value="SSO0115"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0115"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.109"/>
    <dbReference type="eggNOG" id="arCOG01875">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107444_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXY1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16400">
        <property type="entry name" value="ParB_Srx_like_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1760.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023098">
        <property type="entry name" value="SerK/SbnI_C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ParB-like N-terminal" evidence="5">
        <location>
            <begin position="7"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="194" mass="22408" checksum="6F0082097917B78C" modified="2011-05-31" version="1">MSTIRIEMEKLGDLIPHEDILIDKVVNISFEVQKIRAIKPIIVDENTHVILDGHHRFTAALRLSLPKIPVIYVNYNSTSISVNVWYRRFSKPYIVKSVLSAIYSNGKICAKFDSIRICDDDLYRLYWKLEKVESFLHSIGINVEKDIVGHLEPPSIYKEDVISIANRGLRFPPKTTRHVYEFIIPQKAISIDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LY07</accession>
    <name>Q7LY07_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transporter, ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0053</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75436">
        <property type="entry name" value="S75436"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988851.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LY07"/>
    <dbReference type="FunCoup" id="Q7LY07">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0053"/>
    <dbReference type="PaxDb" id="273057-SSO0053"/>
    <dbReference type="EnsemblBacteria" id="AAK40417">
        <property type="protein sequence ID" value="AAK40417"/>
        <property type="gene ID" value="SSO0053"/>
    </dbReference>
    <dbReference type="GeneID" id="1455308"/>
    <dbReference type="KEGG" id="sso:SSO0053"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.55"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q7LY07"/>
    <dbReference type="PhylomeDB" id="Q7LY07"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43038:SF8">
        <property type="entry name" value="ABC-TYPE MULTIDRUG TRANSPORT SYSTEM, ATPASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43038">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY H, MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="6"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="246" mass="27837" checksum="EF5C3AFE549EC44F" modified="2011-05-31" version="1">MTNVIVEVKDLYKEYKDKEVLRGISFTVYKGEIFSLLGPNGAGKTTTVKILSCVLKPTRGEVKVIGYKVPNECGKIRERIGVMPQDYQGFLDLTVRENIEYFVKLYNGKETQVDELISLLDLEKVKNQKLRYLSGGYVRRVGIASAFAGSQEILFLDEPTVGLDPKARRGFWEIIKMMKNKGVTIFLTTHYLDEAQKLSDRVAILYGGKLIKVSTADEIMAEFKATNLEDAYLELIKSLEDSENNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LYJ5</accession>
    <name>Q7LYJ5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73079">
        <property type="entry name" value="S73079"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989801.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LYJ5"/>
    <dbReference type="STRING" id="273057.SSO2083"/>
    <dbReference type="PaxDb" id="273057-SSO2083"/>
    <dbReference type="EnsemblBacteria" id="AAK42263">
        <property type="protein sequence ID" value="AAK42263"/>
        <property type="gene ID" value="SSO2083"/>
    </dbReference>
    <dbReference type="GeneID" id="1453590"/>
    <dbReference type="KEGG" id="sso:SSO2083"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2160"/>
    <dbReference type="eggNOG" id="arCOG07198">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1590955_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LYJ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="167" mass="17608" checksum="573F551DBF998AA9" modified="2011-05-31" version="1">MAIGKTVLIVGAIILIVGIALFFIGGYLASSGLIKIVNTLSTASPTTLQPGSSQDLGVPSKLSILLYNTSSGQVLKILQEVNGTNQSVPQIAEKGYILALLSPKYDAIMVNNLTTPISVKYVLSQEFASSLINVALYTGLGFFLAIIGVIVLLVGLVLFLRGRGKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TV5</accession>
    <name>Q97TV5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISC1048</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="4">Transposase in transposon ISC1048</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1200</name>
        <name evidence="4" type="ordered locus">SSO1238</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90278">
        <property type="entry name" value="C90278"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923242.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TV5"/>
    <dbReference type="STRING" id="273057.SSO1200"/>
    <dbReference type="PaxDb" id="273057-SSO1200"/>
    <dbReference type="EnsemblBacteria" id="AAK41448">
        <property type="protein sequence ID" value="AAK41448"/>
        <property type="gene ID" value="SSO1200"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41482">
        <property type="protein sequence ID" value="AAK41482"/>
        <property type="gene ID" value="SSO1238"/>
    </dbReference>
    <dbReference type="GeneID" id="1454261"/>
    <dbReference type="KEGG" id="sso:SSO1200"/>
    <dbReference type="KEGG" id="sso:SSO1238"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1199"/>
    <dbReference type="eggNOG" id="arCOG02130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056788_0_1_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052709">
        <property type="entry name" value="Transposase-MT_Hybrid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025959">
        <property type="entry name" value="Winged_HTH_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46060">
        <property type="entry name" value="MARINER MOS1 TRANSPOSASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46060:SF1">
        <property type="entry name" value="MARINER MOS1 TRANSPOSASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13551">
        <property type="entry name" value="HTH_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13592">
        <property type="entry name" value="HTH_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Winged helix-turn helix" evidence="2">
        <location>
            <begin position="94"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="158"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="327" mass="38287" checksum="9177CA38414455B7" modified="2001-10-01" version="1">MQDLVQAYKEEKNARIKERILAVKLHVVDGKSEKEVSKMLNKGYSTIKLWIGKYKKEGLDGLKDKPRSGRPRKVEEEKIKQILEDKPQKYGIQQEYWTMKTLKIALQEQGIEYKKSRLYELVHELGYNLVKPRPTNIQAEKDKWEDFKKIKKLERKALFFLDECRTVISTSIKKVLAKVGSKPVMRVNIGFSSIYVILAINAWTGEVVVSLAKRPNSESVKYFLRYFKRRVGSGRVYMVMDNYSPHKTKGTLEVCRRKGIHPVFTPPYSPELNMAEAVFKSLKNYMSNKIFYTIEDVKNCIKQFFEENKYRFNLNAITYLGLDKIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TW3</accession>
    <name>Q97TW3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Second ORF in transposon ISC1078</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0041</name>
        <name evidence="3" type="ordered locus">SSO1263</name>
        <name evidence="4" type="ordered locus">SSO1385</name>
        <name evidence="5" type="ordered locus">SSO2993</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90280">
        <property type="entry name" value="F90280"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TW3"/>
    <dbReference type="STRING" id="273057.SSO0041"/>
    <dbReference type="PaxDb" id="273057-SSO0041"/>
    <dbReference type="EnsemblBacteria" id="AAK40406">
        <property type="protein sequence ID" value="AAK40406"/>
        <property type="gene ID" value="SSO0041"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41501">
        <property type="protein sequence ID" value="AAK41501"/>
        <property type="gene ID" value="SSO1263"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41619">
        <property type="protein sequence ID" value="AAK41619"/>
        <property type="gene ID" value="SSO1385"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK43099">
        <property type="protein sequence ID" value="AAK43099"/>
        <property type="gene ID" value="SSO2993"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0041"/>
    <dbReference type="KEGG" id="sso:SSO1263"/>
    <dbReference type="KEGG" id="sso:SSO1385"/>
    <dbReference type="KEGG" id="sso:SSO2993"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1260"/>
    <dbReference type="eggNOG" id="arCOG02129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431705_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="22"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="197" mass="22681" checksum="360C05DB211AD670" modified="2001-10-01" version="1">MGEGTRGEAFKKIREGYKVHIFDESYVSYINKGKGWMKVGGGIKVNPKRKRFAVVGGITISKEGITFSYSTYKKPSLNSKDIILYLRKVIKDEKTVIVMDNARIHGNEVREFLEENGVEFVYLPPYSPDKSPAEGPWAVLKKRLYSKIYTDFETLMWDATRFLRSISKRVNDLTYSVKRCGEPWTSILSEVFLPFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TW5</accession>
    <name>Q97TW5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposase ISC1290</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0851</name>
        <name evidence="3" type="ordered locus">SSO1612</name>
        <name evidence="4" type="ordered locus">SSO1946</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90236">
        <property type="entry name" value="B90236"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923140.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TW5"/>
    <dbReference type="PaxDb" id="273057-SSO0851"/>
    <dbReference type="EnsemblBacteria" id="AAK41145">
        <property type="protein sequence ID" value="AAK41145"/>
        <property type="gene ID" value="SSO0851"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41827">
        <property type="protein sequence ID" value="AAK41827"/>
        <property type="gene ID" value="SSO1612"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK42139">
        <property type="protein sequence ID" value="AAK42139"/>
        <property type="gene ID" value="SSO1946"/>
    </dbReference>
    <dbReference type="GeneID" id="1455112"/>
    <dbReference type="KEGG" id="sso:SSO0851"/>
    <dbReference type="KEGG" id="sso:SSO1612"/>
    <dbReference type="KEGG" id="sso:SSO1946"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1654"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075479_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="307" mass="36660" checksum="27B9C5D321623C64" modified="2001-10-01" version="1">MKHWIEYYNGPVPYEHLSARHKTLVKMNYMLITSEVLSRLSDLFILRALIVMIVWTCSYRDVRSYYESDVVVRWFLGEYKSKSEIHRRAKKFRGEVKTLFKEYAKELEGKMSRLADYLPSSALYGKVGKLWIVDSFLIEVPFGKRNKETLKKKFELDLRQRKYREAANTLFFYIKCKARRRFKGEFTKKRNRSYFGFKVFNLMSPTMIVHEIQVELANFPDNKVGFSRSGYKVVDRGFVGKSSTWLIGFSSFRRYVEFFGIFLRRYWRPYATEKGMVEFFVYVIALIYNSYIYTSVLSRVPESQLAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TZ3</accession>
    <name>Q97TZ3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase M48 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO3238</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90509">
        <property type="entry name" value="D90509"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989672.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TZ3"/>
    <dbReference type="STRING" id="273057.SSO3238"/>
    <dbReference type="PaxDb" id="273057-SSO3238"/>
    <dbReference type="DNASU" id="1453250"/>
    <dbReference type="EnsemblBacteria" id="AAK43331">
        <property type="protein sequence ID" value="AAK43331"/>
        <property type="gene ID" value="SSO3238"/>
    </dbReference>
    <dbReference type="GeneID" id="1453250"/>
    <dbReference type="KEGG" id="sso:SSO3238"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3338"/>
    <dbReference type="eggNOG" id="arCOG01333">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1529223_0_0_2"/>
    <dbReference type="InParanoid" id="Q97TZ3"/>
    <dbReference type="PhylomeDB" id="Q97TZ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051156">
        <property type="entry name" value="Mito/Outer_Membr_Metalloprot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726:SF1">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="8">
        <location>
            <begin position="38"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK43331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="182" mass="21005" checksum="1F1962B811B11BB1" modified="2001-10-01" version="1">MDLIITFGLLTLVVLLEFIVVPAIILKRGTKFSTIYNYPIYIINSTEINAYSLTSVWGKFIVLTRGLVNGEDEEHIKAAIMHEVGHLKLNHHVKMSLYIISVIMVFTYLLGVNMLTLIPFALVALLVQRYLQRRLELGADRFALRFINKKMLEDLITKYDMKETTFLSTHPNIHVRLKNINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U00</accession>
    <name>Q97U00_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO3230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90508">
        <property type="entry name" value="E90508"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U00"/>
    <dbReference type="FunCoup" id="Q97U00">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3230"/>
    <dbReference type="PaxDb" id="273057-SSO3230"/>
    <dbReference type="EnsemblBacteria" id="AAK43324">
        <property type="protein sequence ID" value="AAK43324"/>
        <property type="gene ID" value="SSO3230"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3230"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3332"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_12_1_2"/>
    <dbReference type="InParanoid" id="Q97U00"/>
    <dbReference type="PhylomeDB" id="Q97U00"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09836">
        <property type="entry name" value="CBS_pair_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="37"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="100"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="156" mass="17624" checksum="4D65449513E8A00E" modified="2001-10-01" version="1">MSFLYHIIGKSIYSLNLYYLFSYTMINMKEEFVKEYMKTRVITVGRNTMLKEVTRIMTDNNVGSVIVVDNGKPIGIITERDVVRAIGKGKSLDTIAEEIMTASLITIKEDSPITGALSLMRTYNIRHLPVIDYDGNLRGIISIRDIARAIDDILEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2013-06-26" modified="2025-06-18" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U28</accession>
    <name>KDGA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase</fullName>
            <ecNumber evidence="2">4.1.2.55</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">eda</name>
        <name type="ordered locus">SSO3197</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Biochem. J." volume="390" first="529" last="540">
            <title>The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluation.</title>
            <authorList>
                <person name="Ahmed H."/>
                <person name="Ettema T.J."/>
                <person name="Tjaden B."/>
                <person name="Geerling A.C."/>
                <person name="van der Oost J."/>
                <person name="Siebers B."/>
            </authorList>
            <dbReference type="PubMed" id="15869466"/>
            <dbReference type="DOI" id="10.1042/BJ20041711"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the degradation of glucose and galactose via the Entner-Doudoroff pathway. Catalyzes the reversible cleavage of 2-keto-3-deoxy-6-phosphogluconate (KDPG) and 2-keto-3-deoxygluconate (KDG) forming pyruvate and glyceraldehyde 3-phosphate or glyceraldehyde, respectively. It is also able to catalyze the reversible cleavage of 2-keto-3-deoxy-6-phosphogalactonate (KDPGal) and 2-keto-3-deoxygalactonate (KDGal).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-dehydro-3-deoxy-6-phospho-D-gluconate = D-glyceraldehyde 3-phosphate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:17089"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57569"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.55"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-dehydro-3-deoxy-6-phospho-D-galactonate = D-glyceraldehyde 3-phosphate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:24464"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:58298"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.55"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Carbohydrate acid metabolism; 2-dehydro-3-deoxy-D-gluconate degradation; D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DapA family. KDPG aldolase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.55" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90504">
        <property type="entry name" value="G90504"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U28"/>
    <dbReference type="SMR" id="Q97U28"/>
    <dbReference type="FunCoup" id="Q97U28">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3197"/>
    <dbReference type="PaxDb" id="273057-SSO3197"/>
    <dbReference type="EnsemblBacteria" id="AAK43294">
        <property type="protein sequence ID" value="AAK43294"/>
        <property type="gene ID" value="SSO3197"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3197"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3300"/>
    <dbReference type="eggNOG" id="arCOG04172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_5_1_2"/>
    <dbReference type="InParanoid" id="Q97U28"/>
    <dbReference type="PhylomeDB" id="Q97U28"/>
    <dbReference type="BRENDA" id="4.1.2.55">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00856">
        <property type="reaction ID" value="UER00829"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008674">
        <property type="term" value="F:2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008675">
        <property type="term" value="F:2-dehydro-3-deoxy-phosphogluconate aldolase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00953">
        <property type="entry name" value="KDG_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000289">
        <property type="entry name" value="2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053415">
        <property type="entry name" value="ED_pathway_aldolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040954">
        <property type="entry name" value="Arch_KDGaldase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128:SF66">
        <property type="entry name" value="4-HYDROXY-2-OXOGLUTARATE ALDOLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="DIHYDRODIPICOLINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000422655" description="2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="57"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="144"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="169"/>
            <end position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Proton shuttle" evidence="1">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15869466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="308" mass="34795" checksum="6A5DE1EFB91D3C80" modified="2001-10-01" version="1">MGRQGNLEELWCLRMPEIITPIITPFTKDNRIDKEKLKIHAENLIRKGIDKLFVNGTTGLGPSLSPEEKLENLKAVYDVTNKIIFQVGGLNLDDAIRLAKLSKDFDIVGIASYAPYYYPRMSEKHLVKYFKTLCEVSPHPVYLYNYPTATGKDIDAKVAKEIGCFTGVKDTIENIIHTLDYKRLNPNMLVYSGSDMLIATVASTGLDGNVAAGSNYLPEVTVTIKKLAMERKIDEALKLQFLHDEVIEASRIFGSLSSNYVLTKYFQGYDLGYPRPPIFPLDDEEERQLIKKVEGIRAKLVELKILKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U73</accession>
    <name>Q97U73_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in partial transposon ISC1160</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO12068</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99499">
        <property type="entry name" value="A99499"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U73"/>
    <dbReference type="PaxDb" id="273057-SSO12068"/>
    <dbReference type="EnsemblBacteria" id="AAK43248">
        <property type="protein sequence ID" value="AAK43248"/>
        <property type="gene ID" value="SSO12068"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO12068"/>
    <dbReference type="HOGENOM" id="CLU_3057320_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U73"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="53" mass="6517" checksum="F3CBD7E5564EE710" modified="2001-10-01" version="1">MSNIRFWKNNRNYSFVRNSTTKERFKVRRSILPRSEFQMIILDFPHLNSANRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U81</accession>
    <name>Q97U81_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99498">
        <property type="entry name" value="A99498"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990913.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U81"/>
    <dbReference type="STRING" id="273057.SSO3142"/>
    <dbReference type="PaxDb" id="273057-SSO3142"/>
    <dbReference type="EnsemblBacteria" id="AAK43240">
        <property type="protein sequence ID" value="AAK43240"/>
        <property type="gene ID" value="SSO3142"/>
    </dbReference>
    <dbReference type="GeneID" id="1453159"/>
    <dbReference type="KEGG" id="sso:SSO3142"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3246"/>
    <dbReference type="eggNOG" id="arCOG03854">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_584789_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U81"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="467" mass="52320" checksum="B4FEC15328D7F234" modified="2001-10-01" version="1">MPSAVTNLLIIIATVIITLSAFAIYSTFLSVQGVTFLQEENVISISKTVQVVVSQVSFKGLPPSYNYFNVSYLLWINSPTKYVTVIPFVASPRLNPFYTLPSNSQNASIFFSTPNGYTSLKSFVFSNNVYLPQQSQLIAKTNGVYAYNLTSNRSYILSAKVSNSQIIVLWILYYYQGKWYRLDYTYLNPSNAGVGVYVLSGSGNYVGNSKNTNFTPPHQVANQKGLGFGLWFKLISNATTKTYLFNETITPTDNKNFSILAWVIGNKLYVGIYSSLGNTGNKLSTNQTYLLTLSPNNWYFINFSLGSILDFSKSPGSQLSPSQSVNFTIYNLSSQKWLNTTTLGVGESNGYISIVKFGSSSATITISQAYFVTLQNKPSLTNFYNVSSIMFKDGPLYNNTYNYNRTIVSSNNLYAIGYWYFIYPSYPPPSTIPGILWYWPIGNGQSNTKYPQIYYIPEQGYNTYVLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-11" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UB2</accession>
    <name>ILVD_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroxy-acid dehydratase</fullName>
            <shortName evidence="1">DAD</shortName>
            <ecNumber evidence="1">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ilvD</name>
        <name type="ordered locus">SSO3107</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:27695"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IlvD/Edd family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99494">
        <property type="entry name" value="B99494"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990927.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UB2"/>
    <dbReference type="SMR" id="Q97UB2"/>
    <dbReference type="FunCoup" id="Q97UB2">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3107"/>
    <dbReference type="PaxDb" id="273057-SSO3107"/>
    <dbReference type="EnsemblBacteria" id="AAK43209">
        <property type="protein sequence ID" value="AAK43209"/>
        <property type="gene ID" value="SSO3107"/>
    </dbReference>
    <dbReference type="GeneID" id="7808534"/>
    <dbReference type="GeneID" id="7810155"/>
    <dbReference type="KEGG" id="sso:SSO3107"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3213"/>
    <dbReference type="eggNOG" id="arCOG04045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014271_4_2_2"/>
    <dbReference type="InParanoid" id="Q97UB2"/>
    <dbReference type="PhylomeDB" id="Q97UB2"/>
    <dbReference type="BRENDA" id="4.2.1.9">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009082">
        <property type="term" value="P:branched-chain amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.80:FF:000001">
        <property type="entry name" value="Dihydroxy-acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="entry name" value="IlvD/EDD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050165">
        <property type="entry name" value="DHAD_IlvD/Edd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056740">
        <property type="entry name" value="ILV_EDD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="ILV_EDD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002068">
        <property type="entry name" value="PRK00911.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000:SF5">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24877">
        <property type="entry name" value="ILV_EDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="IlvD/EDD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000103551" description="Dihydroxy-acid dehydratase">
        <location>
            <begin position="1"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="1">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00012"/>
        </source>
    </evidence>
    <sequence length="558" mass="59422" checksum="059B2D16BAC62643" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UD9</accession>
    <name>Q97UD9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO11920</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90490">
        <property type="entry name" value="G90490"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989096.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UD9"/>
    <dbReference type="STRING" id="273057.SSO11920"/>
    <dbReference type="PaxDb" id="273057-SSO11920"/>
    <dbReference type="DNASU" id="1453101"/>
    <dbReference type="EnsemblBacteria" id="AAK43182">
        <property type="protein sequence ID" value="AAK43182"/>
        <property type="gene ID" value="SSO11920"/>
    </dbReference>
    <dbReference type="GeneID" id="7808560"/>
    <dbReference type="KEGG" id="sso:SSO11920"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3184"/>
    <dbReference type="eggNOG" id="arCOG00271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2366349_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UD9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="76" mass="8438" checksum="20F6759DBB068065" modified="2001-10-01" version="1">MIPILPFYGIHDLNLISIFAILGLVFILTLIGQDSVIYTANKLPISLVTSVELIEPVIVTLLAILIYHQIPNLQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UF4</accession>
    <name>Q97UF4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Arabinose ABC transporter, permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO3067</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90488">
        <property type="entry name" value="H90488"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990875.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UF4"/>
    <dbReference type="FunCoup" id="Q97UF4">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3067"/>
    <dbReference type="TCDB" id="3.A.1.1.14">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3067"/>
    <dbReference type="EnsemblBacteria" id="AAK43167">
        <property type="protein sequence ID" value="AAK43167"/>
        <property type="gene ID" value="SSO3067"/>
    </dbReference>
    <dbReference type="GeneID" id="7798450"/>
    <dbReference type="KEGG" id="sso:SSO3067"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3167"/>
    <dbReference type="eggNOG" id="arCOG00157">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UF4"/>
    <dbReference type="PhylomeDB" id="Q97UF4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054947">
        <property type="entry name" value="GlcT_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052730">
        <property type="entry name" value="Sugar_ABC_transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040931">
        <property type="entry name" value="ABC_arch_GlcT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43759:SF1">
        <property type="entry name" value="GLUCOSE IMPORT SYSTEM PERMEASE PROTEIN GLCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43759">
        <property type="entry name" value="TREHALOSE TRANSPORT SYSTEM PERMEASE PROTEIN SUGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="62"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="285" mass="31996" checksum="30A01BF614581D75" modified="2001-10-01" version="1">MRKGAILMSIPGFIFAAFLIYLLALNVYFSLLNWSLFNLQPSFAGLGTYQTLFSEYFFSLAVTHTIVYTIAAVAIGNILGILGAGILYFLKSNTRRAIYLSVFLYPLAVPSSTYAITWQWLFNPQTGINLVLRIFHFPNIIWLTTQPTIYAGMIIIETWAYTGLAVLFFLASFMSVDKSIIEAAKIDGANNFYLLFRIILPNSMNGLIVSTALLFLFSFRIFTVPFTIGGPTNPNMMSLVEYIYILFSTEYFSLSSALSSLVALIAAVVIIPYAILGLKRWVFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UG9</accession>
    <name>Q97UG9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Xylose isomerase-like TIM barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90486">
        <property type="entry name" value="G90486"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UG9"/>
    <dbReference type="SMR" id="Q97UG9"/>
    <dbReference type="STRING" id="273057.SSO3050"/>
    <dbReference type="PaxDb" id="273057-SSO3050"/>
    <dbReference type="DNASU" id="1453069"/>
    <dbReference type="EnsemblBacteria" id="AAK43150">
        <property type="protein sequence ID" value="AAK43150"/>
        <property type="gene ID" value="SSO3050"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3050"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3148"/>
    <dbReference type="eggNOG" id="arCOG01902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085355_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UG9"/>
    <dbReference type="PhylomeDB" id="Q97UG9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050312">
        <property type="entry name" value="IolE/XylAMocC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110">
        <property type="entry name" value="HYDROXYPYRUVATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110:SF41">
        <property type="entry name" value="INOSOSE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="1">
        <location>
            <begin position="26"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="237" mass="27012" checksum="E116E5AEF87CD0AC" modified="2001-10-01" version="1">MFLSSMKLGIQSYSLRKLTYENALKVMSELGISYVEAFPRHLPPSSNSVKDIINLHKENGVKVIAHGVNHLKSDKKELRSIFDFANKVEIEVITADPDEDCLNLMSDLVKEYDIKVAIHNHGPYHRWGSYKKIYEFTKNLDYRVGMCLDLAHLFRYGENPIEAVNTLRDRIHDIHVKDVNNKGEDVIVGSGILDVKGVIGKVKELNLDIPLMIEYEPEPENPLPGIVKSLEYVKKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UI6</accession>
    <name>Q97UI6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO11766</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90484">
        <property type="entry name" value="E90484"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UI6"/>
    <dbReference type="STRING" id="273057.SSO11766"/>
    <dbReference type="PaxDb" id="273057-SSO11766"/>
    <dbReference type="EnsemblBacteria" id="AAK43132">
        <property type="protein sequence ID" value="AAK43132"/>
        <property type="gene ID" value="SSO11766"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11766"/>
    <dbReference type="eggNOG" id="arCOG09948">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2949477_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UI6"/>
    <dbReference type="PhylomeDB" id="Q97UI6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="59" mass="6774" checksum="A8E5E3C89930AFAD" modified="2001-10-01" version="1">MGFNDVFLLMGYTTSRLKNFTAFRKYKGSWGRKHGKSYFGFKVLWKGGLISLGTSRLDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UI7</accession>
    <name>Q97UI7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Third ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90484">
        <property type="entry name" value="D90484"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UI7"/>
    <dbReference type="STRING" id="273057.SSO3030"/>
    <dbReference type="PaxDb" id="273057-SSO3030"/>
    <dbReference type="EnsemblBacteria" id="AAK43131">
        <property type="protein sequence ID" value="AAK43131"/>
        <property type="gene ID" value="SSO3030"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3030"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3125"/>
    <dbReference type="eggNOG" id="arCOG09948">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154343_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UI7"/>
    <dbReference type="PhylomeDB" id="Q97UI7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="99" mass="11140" checksum="FA2C70E8ED5BA1F9" modified="2001-10-01" version="1">MERRTNFVRDFKVGLLSDLAFSFDNVKLMADRAWISRKDVLVKGVGSAGLPVEGSGVKIREGKASSTTLRGVVMEVFFLNFYRDLEILSTRIRTKVLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UR0</accession>
    <name>Q97UR0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbon monoxide dehydrogenase, large chain (CutA-6)</fullName>
            <ecNumber evidence="4">1.2.7.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cutA-6</name>
        <name evidence="4" type="ordered locus">SSO2942</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.4" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90473">
        <property type="entry name" value="G90473"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924045.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UR0"/>
    <dbReference type="FunCoup" id="Q97UR0">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2942"/>
    <dbReference type="PaxDb" id="273057-SSO2942"/>
    <dbReference type="EnsemblBacteria" id="AAK43046">
        <property type="protein sequence ID" value="AAK43046"/>
        <property type="gene ID" value="SSO2942"/>
    </dbReference>
    <dbReference type="GeneID" id="1452965"/>
    <dbReference type="KEGG" id="sso:SSO2942"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3030"/>
    <dbReference type="eggNOG" id="arCOG01167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_2_0_2"/>
    <dbReference type="InParanoid" id="Q97UR0"/>
    <dbReference type="PhylomeDB" id="Q97UR0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:anaerobic carbon-monoxide dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.50">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.365.10">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046867">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908:SF132">
        <property type="entry name" value="ALDEHYDE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="MoCoBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20256">
        <property type="entry name" value="MoCoBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde oxidase/xanthine dehydrogenase a/b hammerhead" evidence="3">
        <location>
            <begin position="10"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="684" mass="75215" checksum="2ACF905C0B2C66D6" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UT5</accession>
    <name>Q97UT5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">uroporphyrinogen-III C-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cysG</name>
        <name evidence="8" type="ordered locus">SSO2908</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.107" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90470">
        <property type="entry name" value="B90470"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993315.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UT5"/>
    <dbReference type="FunCoup" id="Q97UT5">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2908"/>
    <dbReference type="PaxDb" id="273057-SSO2908"/>
    <dbReference type="EnsemblBacteria" id="AAK43017">
        <property type="protein sequence ID" value="AAK43017"/>
        <property type="gene ID" value="SSO2908"/>
    </dbReference>
    <dbReference type="GeneID" id="1452936"/>
    <dbReference type="KEGG" id="sso:SSO2908"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2998"/>
    <dbReference type="eggNOG" id="arCOG00644">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_7_0_2"/>
    <dbReference type="InParanoid" id="Q97UT5"/>
    <dbReference type="PhylomeDB" id="Q97UT5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000001">
        <property type="entry name" value="Siroheme synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790:SF3">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT UROPORPHYRINOGEN III METHYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="7">
        <location>
            <begin position="3"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK43017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="231" mass="25509" checksum="6C3CF65A18B58B53" modified="2001-10-01" version="1">MGKVVLVGAGPGDPELITLKGIKYLQMADVIVYDKLVSRELINYAKPSSFVIFLSNDDIEVDLLRKYALEDKLVIRLKNGDPYIFGRGGKICQELIKDGIECEVIPGVSSVNSVPAYAGIPLTFNGVSDMVTIVSAVSQGGKLFNFQKIPDYGTLVVLMGGRRLEEVSKWLMAKRDPSEDVAVIERGTYLDQKVHVNKLEKLAEVGNNLATPSLLVLGNVVRLREILWKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V70</accession>
    <name>Q97V70_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2764</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90452">
        <property type="entry name" value="D90452"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990239.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V70"/>
    <dbReference type="STRING" id="273057.SSO2764"/>
    <dbReference type="PaxDb" id="273057-SSO2764"/>
    <dbReference type="EnsemblBacteria" id="AAK42875">
        <property type="protein sequence ID" value="AAK42875"/>
        <property type="gene ID" value="SSO2764"/>
    </dbReference>
    <dbReference type="GeneID" id="1452794"/>
    <dbReference type="KEGG" id="sso:SSO2764"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2845"/>
    <dbReference type="eggNOG" id="arCOG03696">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022113_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V70"/>
    <dbReference type="PhylomeDB" id="Q97V70"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051684">
        <property type="entry name" value="Electron_Trans/Redox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30176">
        <property type="entry name" value="FERREDOXIN-TYPE PROTEIN NAPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30176:SF3">
        <property type="entry name" value="FERREDOXIN-TYPE PROTEIN NAPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="494"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="519"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="553"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="588"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="627" mass="71973" checksum="3C03FFFF9A91724C" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-01-31" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VF1</accession>
    <name>APE1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Probable aminopeptidase 1</fullName>
            <ecNumber>3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">ape1</name>
        <name type="ordered locus">SSO2675</name>
        <name type="ORF">C30_037</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90442">
        <property type="entry name" value="B90442"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988593.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VF1"/>
    <dbReference type="SMR" id="Q97VF1"/>
    <dbReference type="FunCoup" id="Q97VF1">
        <property type="interactions" value="170"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2675"/>
    <dbReference type="MEROPS" id="M01.021"/>
    <dbReference type="PaxDb" id="273057-SSO2675"/>
    <dbReference type="EnsemblBacteria" id="AAK42793">
        <property type="protein sequence ID" value="AAK42793"/>
        <property type="gene ID" value="SSO2675"/>
    </dbReference>
    <dbReference type="GeneID" id="1452712"/>
    <dbReference type="KEGG" id="sso:SSO2675"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2757"/>
    <dbReference type="eggNOG" id="arCOG02969">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003705_0_3_2"/>
    <dbReference type="InParanoid" id="Q97VF1"/>
    <dbReference type="PhylomeDB" id="Q97VF1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000012">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000006">
        <property type="entry name" value="Puromycin-sensitive aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF174">
        <property type="entry name" value="PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00142">
        <property type="entry name" value="ZINC_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0031">Aminopeptidase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000095106" description="Probable aminopeptidase 1">
        <location>
            <begin position="1"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="236"/>
            <end position="240"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="784" mass="90143" checksum="45C98433821CD619" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VJ0</accession>
    <name>Q97VJ0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dinitrogenase iron-molybdenum cofactor biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2633</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90437">
        <property type="entry name" value="C90437"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988537.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VJ0"/>
    <dbReference type="STRING" id="273057.SSO2633"/>
    <dbReference type="PaxDb" id="273057-SSO2633"/>
    <dbReference type="EnsemblBacteria" id="AAK42754">
        <property type="protein sequence ID" value="AAK42754"/>
        <property type="gene ID" value="SSO2633"/>
    </dbReference>
    <dbReference type="GeneID" id="1452673"/>
    <dbReference type="KEGG" id="sso:SSO2633"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2716"/>
    <dbReference type="eggNOG" id="arCOG02738">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167327_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VJ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="Nitrogenase accessory factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13556" checksum="BCF7C67EA3BCB04B" modified="2001-10-01" version="1">MRIALTYDKSQELKPLDQAEIIGIIDEEKREVEQYENPGIGSKEATMSVILDLNADAIVVGPKFLCPGSYMMSYGRLRYIPTKYGNLKDVLEHLEEIKKNMKEELEEEMYAEEMEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VJ8</accession>
    <name>Q97VJ8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2626</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90436">
        <property type="entry name" value="C90436"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989352.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VJ8"/>
    <dbReference type="SMR" id="Q97VJ8"/>
    <dbReference type="STRING" id="273057.SSO2626"/>
    <dbReference type="PaxDb" id="273057-SSO2626"/>
    <dbReference type="EnsemblBacteria" id="AAK42746">
        <property type="protein sequence ID" value="AAK42746"/>
        <property type="gene ID" value="SSO2626"/>
    </dbReference>
    <dbReference type="GeneID" id="1452665"/>
    <dbReference type="KEGG" id="sso:SSO2626"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2708"/>
    <dbReference type="eggNOG" id="arCOG01284">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119412_2_1_2"/>
    <dbReference type="InParanoid" id="Q97VJ8"/>
    <dbReference type="PhylomeDB" id="Q97VJ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="ChsH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="ChsH2_Znr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF6">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_ChsH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="2">
        <location>
            <begin position="22"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="ChsH2 C-terminal OB-fold" evidence="1">
        <location>
            <begin position="58"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="14574" checksum="DD39FEB40061480E" modified="2001-10-01" version="1">MQIDAIPLSIKYKIKYPDEFIEAVKRGEIVATKCKNCGSVYFPPQKDCYNCGKNEMEWIKVSNEGEIMTYSVVSQKPQGFENYEDYVIGIVKTKDGINIMAWIKGQPKVGNKVRLTTDGMRIIGEVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VL1</accession>
    <name>Q97VL1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HtrA like serine protease (Periplasmic)</fullName>
            <ecNumber evidence="5">3.4.21.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2612</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1C family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90434">
        <property type="entry name" value="F90434"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989333.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VL1"/>
    <dbReference type="FunCoup" id="Q97VL1">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2612"/>
    <dbReference type="PaxDb" id="273057-SSO2612"/>
    <dbReference type="EnsemblBacteria" id="AAK42733">
        <property type="protein sequence ID" value="AAK42733"/>
        <property type="gene ID" value="SSO2612"/>
    </dbReference>
    <dbReference type="GeneID" id="1454060"/>
    <dbReference type="KEGG" id="sso:SSO2612"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2693"/>
    <dbReference type="eggNOG" id="arCOG02833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020120_2_2_2"/>
    <dbReference type="InParanoid" id="Q97VL1"/>
    <dbReference type="PhylomeDB" id="Q97VL1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051201">
        <property type="entry name" value="Chloro_Bact_Ser_Proteases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43343">
        <property type="entry name" value="PEPTIDASE S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43343:SF3">
        <property type="entry name" value="PROTEASE DO-LIKE 8, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="208"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="297" mass="32202" checksum="48DDA59AE322FEC1" modified="2001-10-01" version="1">MYEDLVERVTPSVVTIITKQIALDQFFMPQVAEGIGSGYSIGKNILITSYHVISNAEEILVISEDGFREEAQVIAINPFHDLAMLRSTIELPSLKLAKECKTGEVVLAVGNPLGLYSVSMGIISSEERAIMTPNGLPIYVVQTDAAVNPGNSGGPLINTRGEVVGTVTAMIREAQNIGFAIPSKLVDSFVKNVMKFGRYIRPYVGIGVIKLNKALATYLGVRKQNGLLVTNIDPNGSAYKYGIRRGDIILKVNNQEVKSPIDLLAVLEEMVGSQINVKMLRDSKEIELSIPVPGLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VL4</accession>
    <name>Q97VL4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Trehalose phosphorylase, putative</fullName>
            <ecNumber evidence="9">2.4.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2609</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="9"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90434">
        <property type="entry name" value="C90434"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VL4"/>
    <dbReference type="FunCoup" id="Q97VL4">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2609"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2609"/>
    <dbReference type="DNASU" id="1454057"/>
    <dbReference type="EnsemblBacteria" id="AAK42730">
        <property type="protein sequence ID" value="AAK42730"/>
        <property type="gene ID" value="SSO2609"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2609"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2690"/>
    <dbReference type="eggNOG" id="arCOG01407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045353_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VL4"/>
    <dbReference type="PhylomeDB" id="Q97VL4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03792">
        <property type="entry name" value="GT4_trehalose_phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052078">
        <property type="entry name" value="Trehalose_Metab_GTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049438">
        <property type="entry name" value="TreT_GT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47779">
        <property type="entry name" value="SYNTHASE (CCG-9), PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12100)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47779:SF1">
        <property type="entry name" value="SYNTHASE (CCG-9), PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12100)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21269">
        <property type="entry name" value="TreT_GT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="4 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Trehalose synthase N-terminal" evidence="8">
        <location>
            <begin position="35"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="7">
        <location>
            <begin position="212"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="417" mass="47626" checksum="3126D5CF83458AA6" modified="2001-10-01" version="1">MVVKMIEKYVEFIGEHELDAIFKIAEKIKDLSILHVNSTKAGGGVAEILNRMLPLMKELGLNVDWKVIKGDNEFFNVTKSFHNSLQNGTGNIPDEYFKIYDKWQEINLSEIPLDYDIMFIHDPQPAGLIKFKKGNSNKWIWRCHIDISNPYPPVWNFLRKYISKYDSMIISVPSFGRDDIEIPQFVIPPSIDPLSIKNRDMSETTILRILYKFGINLEKPLITQVSRFDYAKDPLGVIQAYKLAKRHVDIQLLYVGSPATDDPEGEKVYSEVVKASEGDKDIHLLMLPPYSDLEINVFQTASTVVMQKSIKEGFGLTVSEAMWKRKPVIGGNTGGIPLQVINGITGFLVNSPQGASHYIIYLIRNEEIRRRLGTNAREHIRRNFLITRELRDYLMTIVYVANRGLASLNSHESISNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-01-24" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VQ1</accession>
    <name>Q97VQ1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1359</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2562</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90429">
        <property type="entry name" value="D90429"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VQ1"/>
    <dbReference type="PaxDb" id="273057-SSO2562"/>
    <dbReference type="EnsemblBacteria" id="AAK42691">
        <property type="protein sequence ID" value="AAK42691"/>
        <property type="gene ID" value="SSO2562"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2562"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2645"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067987_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VQ1"/>
    <dbReference type="PhylomeDB" id="Q97VQ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="89"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="285" mass="32812" checksum="84FCC126E9B2BACE" modified="2001-10-01" version="1">MKHENTTKPAKFNRDFARSALKIIYSVLTKILFPEELLSALLKASGSYLSRLGKDGRRALRKLNVVQVEDVRDALRKMGRMTLRGVRDRRVAVDFHVIPQYHANKSFLSRIKPTKGTSWGLVQAAIFLLGRKRRFLDVIPVTVKNVAEGFKAVMEVIVKELEEDKLRLVMVFADKEFAVNEVIRFLLELGLDFVISAKAQMYKKYEGMLQSVDVSFGGVRYTGFLCVRHGTGAYLIILRKEDGKIIAFLVRREIDLHDAIVLAEMYRERWGIENAFRSLEEFRIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-31" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VX5</accession>
    <name>NPD_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent protein deacetylase</fullName>
            <ecNumber evidence="1 2">2.3.1.286</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Regulatory protein SIR2 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>ssSir2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cobB</name>
        <name type="ordered locus">SSO2478</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Science" volume="296" first="148" last="151">
            <title>The interaction of Alba, a conserved archaeal chromatin protein, with Sir2 and its regulation by acetylation.</title>
            <authorList>
                <person name="Bell S.D."/>
                <person name="Botting C.H."/>
                <person name="Wardleworth B.N."/>
                <person name="Jackson S.P."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="11935028"/>
            <dbReference type="DOI" id="10.1126/science.1070506"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>MUTAGENESIS OF HIS-116</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="journal article" date="2022" name="Biomolecules" volume="12" first="0" last="0">
            <title>Interplay between Alba and Cren7 Regulates Chromatin Compaction in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Cajili M.K.M."/>
                <person name="Prieto E.I."/>
            </authorList>
            <dbReference type="PubMed" id="35454068"/>
            <dbReference type="DOI" id="10.3390/biom12040481"/>
        </citation>
        <scope>DEVELOPMENTAL STAGE</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">NAD-dependent protein deacetylase which modulates the activities of several enzymes which are inactive in their acetylated form. Deacetylates the N-terminal lysine residue of albA1, the major archaeal DNA compaction protein and that, in turn, increases albA1's DNA binding affinity, thereby repressing transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(6)-acetyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-acetyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:43636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="ChEBI" id="CHEBI:83767"/>
            <dbReference type="EC" id="2.3.1.286"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="developmental stage">
        <text evidence="4">Expression increases during stationary phase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class U subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.286" evidence="1 2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90419">
        <property type="entry name" value="H90419"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923883.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VX5"/>
    <dbReference type="SMR" id="Q97VX5"/>
    <dbReference type="FunCoup" id="Q97VX5">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2478"/>
    <dbReference type="PaxDb" id="273057-SSO2478"/>
    <dbReference type="EnsemblBacteria" id="AAK42615">
        <property type="protein sequence ID" value="AAK42615"/>
        <property type="gene ID" value="SSO2478"/>
    </dbReference>
    <dbReference type="GeneID" id="1453942"/>
    <dbReference type="KEGG" id="sso:SSO2478"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2556"/>
    <dbReference type="eggNOG" id="arCOG04248">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023643_3_1_2"/>
    <dbReference type="InParanoid" id="Q97VX5"/>
    <dbReference type="PhylomeDB" id="Q97VX5"/>
    <dbReference type="BRENDA" id="2.3.1.B42">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01413">
        <property type="entry name" value="SIR2_Af2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01968">
        <property type="entry name" value="Sirtuin_ClassU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028628">
        <property type="entry name" value="Sirtuin_class_U"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001753">
        <property type="entry name" value="PRK00481.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040867">
        <property type="entry name" value="prot_deacyl_CobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF11">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000110388" description="NAD-dependent protein deacetylase">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>nicotinamide</name>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>nicotinamide</name>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>nicotinamide</name>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Loss of function." evidence="3">
        <original>H</original>
        <variation>Y</variation>
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="11935028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="35454068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="247" mass="27594" checksum="A509E4132914E6AD" modified="2001-10-01" version="1">MIYEKVAEELISSSYTIAFTGAGISTASGIPDFRGPQGLWKKYSPELASIEYFEKDPKNFWGFYSLRMRGLFEAQPNKAHYSLAELEKMGIIKVIITQNIDGLHQKAGSKNVIELHGTMRRSYCVLCLRTYDSLNVLSMIEKGNLPPRCDCGGIIRPDVVLFGEPVKNIYEALSIAYESDLVISIGSSLTVYPANLIPQTVKERGGKLIILNMEETPLDSIADYVVREPVEISLPKILENVRQKILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VX7</accession>
    <name>Q97VX7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CobQ/CobB/MinD/ParA nucleotide binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90419">
        <property type="entry name" value="F90419"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923881.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VX7"/>
    <dbReference type="STRING" id="273057.SSO2475"/>
    <dbReference type="PaxDb" id="273057-SSO2475"/>
    <dbReference type="EnsemblBacteria" id="AAK42613">
        <property type="protein sequence ID" value="AAK42613"/>
        <property type="gene ID" value="SSO2475"/>
    </dbReference>
    <dbReference type="GeneID" id="15296747"/>
    <dbReference type="KEGG" id="sso:SSO2475"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2553"/>
    <dbReference type="eggNOG" id="arCOG07293">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090891_1_0_2"/>
    <dbReference type="InParanoid" id="Q97VX7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="1">
        <location>
            <begin position="6"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="244" mass="27658" checksum="B412EAFF5BDCBC80" modified="2001-10-01" version="1">MERIDIASVKGGVGKSFIAYFLSKTLAASHKILLIDKDLTSTLSRVYNIKGNLLSYLTVGQSSLAYFKEVDSGLTVVNVGCSRKIKNVEPKKIADIYSGFSDHDLMIVDNPSIPSDVCLDTELQAYYTYLNEKRLDYNLILILPPNQLLLDESLAFMQLFKDYIKNELSISLGNDLVNFDVISSVINMYNPRERIDISKIKDLTSKIVKIPFKKEAIYTPFNQLNTPMEISELAKYVLEYLESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VY1</accession>
    <name>Q97VY1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Deoxyribodipyrimidine photolyase (DNA photolyase) (Photoreactivating enzyme). (PhrB)</fullName>
            <ecNumber evidence="8">4.1.99.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">phrB</name>
        <name evidence="8" type="ordered locus">SSO2472</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="4">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DNA photolyase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.3" evidence="8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90419">
        <property type="entry name" value="B90419"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923877.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VY1"/>
    <dbReference type="FunCoup" id="Q97VY1">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2472"/>
    <dbReference type="PaxDb" id="273057-SSO2472"/>
    <dbReference type="EnsemblBacteria" id="AAK42609">
        <property type="protein sequence ID" value="AAK42609"/>
        <property type="gene ID" value="SSO2472"/>
    </dbReference>
    <dbReference type="GeneID" id="1453936"/>
    <dbReference type="KEGG" id="sso:SSO2472"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2549"/>
    <dbReference type="eggNOG" id="arCOG02840">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010348_2_2_2"/>
    <dbReference type="InParanoid" id="Q97VY1"/>
    <dbReference type="PhylomeDB" id="Q97VY1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018394">
        <property type="entry name" value="DNA_photolyase_1_CS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF9">
        <property type="entry name" value="CRYPTOCHROME CIRCADIAN CLOCK 5 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00147">
        <property type="entry name" value="DNAPHOTLYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00394">
        <property type="entry name" value="DNA_PHOTOLYASES_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0157">Chromophore</keyword>
    <keyword evidence="2 4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="1 4 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="7">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="242"/>
            <end position="249"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="339"/>
            <end position="341"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="5">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="5">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="5">
        <location>
            <position position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="433" mass="52017" checksum="43E50D9B9FD23A65" modified="2001-10-01" version="1">MLCLFIFRRDLRLDDNTGLIKALEDCEKVIPAFILDPRQVGNENEYKSEFAINFMINSLNELNDELRKRGSRLYVYFGLAEEVIKNLLKDVDAVYLNEDYTPFSKMRDERIRKYCEDTGKIMKSFEDYLLTSKNDFKNYRNFTTFYNVVKNKQIKKPVQNNYTNYYKNSLGDEHELPPRQGERGGRKEGIKLIERARQINYDRKDFVAEDNRTFLSPHLKFGTLSIREVYYSLLDSQAIIRQLYWRDFYTLLAYYNERVFHEPLKREYNCIEWENNERLFQAWLEGKTGYPIIDAGMRQLNRTGDMPNRVRMLTAFFLVKVLIIDWRIGEKYFASKLIDYDPSVNNGNWQWIASVGTDYIFRVFDPWKQQVTYDPEAKYIKRWVDELESYDAEIIHNAYKYTLKSYPKPIVDWRIRVNLAKRLYEVCRKSKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W07</accession>
    <name>Q97W07_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutamine synthetase (Glutamate ammonia ligase) (GS). (GlnA-2)</fullName>
            <ecNumber evidence="5">6.3.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">glnA-2</name>
        <name evidence="5" type="ordered locus">SSO2440</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90415">
        <property type="entry name" value="H90415"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993127.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W07"/>
    <dbReference type="SMR" id="Q97W07"/>
    <dbReference type="FunCoup" id="Q97W07">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2440"/>
    <dbReference type="PaxDb" id="273057-SSO2440"/>
    <dbReference type="EnsemblBacteria" id="AAK42583">
        <property type="protein sequence ID" value="AAK42583"/>
        <property type="gene ID" value="SSO2440"/>
    </dbReference>
    <dbReference type="GeneID" id="1453910"/>
    <dbReference type="KEGG" id="sso:SSO2440"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2521"/>
    <dbReference type="eggNOG" id="arCOG01909">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_6_0_2"/>
    <dbReference type="InParanoid" id="Q97W07"/>
    <dbReference type="PhylomeDB" id="Q97W07"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000024">
        <property type="entry name" value="Glutamine synthetase catalytic region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785">
        <property type="entry name" value="GAMMA-GLUTAMYLPUTRESCINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785:SF12">
        <property type="entry name" value="TYPE-1 GLUTAMINE SYNTHETASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS catalytic" evidence="4">
        <location>
            <begin position="109"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="425" mass="48585" checksum="68D91EE794B5F94B" modified="2001-10-01" version="1">MSKENIFDALKSGKIDYVRVEFVDILGNTKGRSLRRAEFENVINDNKGVDYPESLALMDYKDRPIKSRYEDIIAIPDLNTFVAIPYLERTARVLSFLAQPDGLPYPYCSRSILNKAIEKLKEAGYTLQVSFEPTFYLLNSALNPADYSKAFSLEGLLEQQNFLKLLIKYLEEIDIKVETINKHYGPGQYEVKLSQKPVLEAADSLISSREVIRDTAKMNNVIATFMPKPFKDYPSSSMDITLSLQTVDGKDIMYDPNDSKGIGLSKIAYNFISGILEHLPSILSIAAPTVNSYKRFKEVVTPNMPGIGSERHYIVRLPSYYKDTHQVEFRLADPLANPYILLASMIYAGLDGIERNLMANVNEYYGTLPQTLKESLSRLENDTYMKYNLGQDLISIYINIKNIEIEEYESQITEWERDYYLKAGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W53</accession>
    <name>Q97W53_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type II secretion system protein, virB homolog (GspE-3)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gspE-3</name>
        <name evidence="2" type="ordered locus">SSO2387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90409">
        <property type="entry name" value="H90409"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W53"/>
    <dbReference type="STRING" id="273057.SSO2387"/>
    <dbReference type="PaxDb" id="273057-SSO2387"/>
    <dbReference type="EnsemblBacteria" id="AAK42535">
        <property type="protein sequence ID" value="AAK42535"/>
        <property type="gene ID" value="SSO2387"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2387"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2469"/>
    <dbReference type="eggNOG" id="arCOG01817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_982184_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W53"/>
    <dbReference type="PhylomeDB" id="Q97W53"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF6">
        <property type="entry name" value="TYPE IV PILUS RETRACTATION ATPASE PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="283" mass="32381" checksum="53C7711A249A81CF" modified="2001-10-01" version="1">MGEWYIMSKIFKFPLQIGRGSVKQLPITDLPITLYPVTPLPEEVTTIVADYEVNILNLVPEDIKSNLTRNNIELILPNPHVFITFDERKGIYKYVLLEPPVNEMIYNIYNIFIEEVERELLSKNPSLDLAKIIFELDKKRSGLKIIQEKRGDIYVLSTNARVTLYYLLRNMFGYNVLTPLVADKNIEDISVPGLNNPVYVYHRSYEYIPTNIIFTKNMQVSPQLNIMIDGEELLDQLVLRMLSTTGKSISVAEPIQDGMLPNGDRVAATFSGRPKITSIKINL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W56</accession>
    <name>RNH2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease HII</fullName>
            <shortName evidence="1">RNase HII</shortName>
            <ecNumber evidence="1">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnhB</name>
        <name type="ordered locus">SSO2384</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase HII family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90409">
        <property type="entry name" value="E90409"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989457.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W56"/>
    <dbReference type="SMR" id="Q97W56"/>
    <dbReference type="FunCoup" id="Q97W56">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2384"/>
    <dbReference type="PaxDb" id="273057-SSO2384"/>
    <dbReference type="EnsemblBacteria" id="AAK42532">
        <property type="protein sequence ID" value="AAK42532"/>
        <property type="gene ID" value="SSO2384"/>
    </dbReference>
    <dbReference type="GeneID" id="1453859"/>
    <dbReference type="GeneID" id="44128106"/>
    <dbReference type="KEGG" id="sso:SSO2384"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2466"/>
    <dbReference type="eggNOG" id="arCOG04121">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036532_0_4_2"/>
    <dbReference type="InParanoid" id="Q97W56"/>
    <dbReference type="PhylomeDB" id="Q97W56"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07180">
        <property type="entry name" value="RNase_HII_archaea_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.460:FF:000001">
        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.460">
        <property type="entry name" value="Ribonuclease hii. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00052_A">
        <property type="entry name" value="RNase_HII_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004649">
        <property type="entry name" value="RNase_H2_suA"/>
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        <property type="entry name" value="RNase_HII/HIII"/>
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        <property type="entry name" value="RNase_HII/HIII_dom"/>
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        <property type="entry name" value="RNase_HII_arc"/>
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        <property type="entry name" value="RNase_HII_hlx-loop-hlx_cap_dom"/>
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    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
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    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
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    <dbReference type="NCBIfam" id="TIGR00729">
        <property type="entry name" value="ribonuclease HII"/>
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    <dbReference type="PANTHER" id="PTHR10954:SF23">
        <property type="entry name" value="RIBONUCLEASE"/>
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        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
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        <property type="entry name" value="Ribonuclease H-like"/>
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        <property type="entry name" value="RNASE_H_2"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
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            <begin position="1"/>
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    <feature type="domain" description="RNase H type-2" evidence="2">
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    <feature type="binding site" evidence="1">
        <location>
            <position position="6"/>
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        <ligand>
            <name>a divalent metal cation</name>
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        <location>
            <position position="7"/>
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        <ligand>
            <name>a divalent metal cation</name>
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    <feature type="binding site" evidence="1">
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        <ligand>
            <name>a divalent metal cation</name>
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    <evidence type="ECO:0000255" key="1">
        <source>
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    <evidence type="ECO:0000255" key="2">
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    <sequence length="212" mass="23925" checksum="2FCBF6D2623A488C" modified="2001-10-01" version="1">MRVGIDEAGRGALIGPMIVAGVVISDTKLKFLKGIGVKDSKQLTRERREKLFDIVANTVEAFTVVKVFPYEIDNYNLNDLTYDAVSKIILSLSSFNPEIVTVDKVGDEKPVIELINKLGYKSNVVHKADVLFVEASAASIIAKVIRDNYIDELKQVYGDFGSGYPADPRTIKWLKSFYEKNPNPPPIIRRSWKILRSTAPLYYISKEGRRLW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-09-05" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W92</accession>
    <name>HEXPP_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Hexaprenyl pyrophosphate synthase</fullName>
            <shortName>HexPP</shortName>
            <ecNumber evidence="3 4">2.5.1.82</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Medium-chain HexPP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">gdS-2</name>
        <name type="ordered locus">SSO2345</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="J. Bacteriol." volume="184" first="615" last="620">
            <title>Novel medium-chain prenyl diphosphate synthase from the thermoacidophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Hemmi H."/>
                <person name="Ikejiri S."/>
                <person name="Yamashita S."/>
                <person name="Nishino T."/>
            </authorList>
            <dbReference type="PubMed" id="11790729"/>
            <dbReference type="DOI" id="10.1128/JB.184.3.615-620.2002"/>
        </citation>
        <scope>FUNCTION AS A HEXAPRENYL PYROPHOSPHATE SYNTHASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>COFACTOR</scope>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="journal article" date="2005" name="J. Bacteriol." volume="187" first="8137" last="8148">
            <title>Homodimeric hexaprenyl pyrophosphate synthase from the thermoacidophilic crenarchaeon Sulfolobus solfataricus displays asymmetric subunit structures.</title>
            <authorList>
                <person name="Sun H.Y."/>
                <person name="Ko T.P."/>
                <person name="Kuo C.J."/>
                <person name="Guo R.T."/>
                <person name="Chou C.C."/>
                <person name="Liang P.H."/>
                <person name="Wang A.H."/>
            </authorList>
            <dbReference type="PubMed" id="16291686"/>
            <dbReference type="DOI" id="10.1128/jb.187.23.8137-8148.2005"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF MUTANT CYS-81</scope>
        <scope>MUTAGENESIS OF TYR-124; LEU-164 AND TYR-174</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes consecutive E-type condensation of two isopentenyl pyrophosphate (IPP) molecules with an allylic substrate such as geranylgeranyl diphosphate (GGPP), farnesyl diphosphate (FPP) or geranyl diphosphate (GPP) to yield the medium-chain product trans-C30-hexaprenyl pyrophosphate (HexPP). GGPP is the physiological substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>2 isopentenyl diphosphate + (2E,6E,10E)-geranylgeranyl diphosphate = all-trans-hexaprenyl diphosphate + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27555"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58179"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="2.5.1.82"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="3">Optimum pH is 6.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="3">Highly thermostable, remains fully active after 2 hours at 80 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="4 6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.82" evidence="3 4"/>
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        <property type="protein sequence ID" value="AAK42496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="A99405"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="PDB" id="2AZJ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=1-281"/>
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    <dbReference type="PDB" id="2AZK">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B=1-281"/>
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    <dbReference type="PDBsum" id="2AZJ"/>
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        <property type="interactions" value="16"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="AAK42496">
        <property type="protein sequence ID" value="AAK42496"/>
        <property type="gene ID" value="SSO2345"/>
    </dbReference>
    <dbReference type="GeneID" id="1453823"/>
    <dbReference type="GeneID" id="44128070"/>
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    <dbReference type="PATRIC" id="fig|273057.12.peg.2429"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_5_0_2"/>
    <dbReference type="InParanoid" id="Q97W92"/>
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        <property type="organism ID" value="6163"/>
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        <property type="component" value="Chromosome"/>
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        <property type="term" value="F:hexaprenyl diphosphate synthase (geranylgeranyl-diphosphate specific) activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000418599" description="Hexaprenyl pyrophosphate synthase">
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            <begin position="1"/>
            <end position="281"/>
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    <feature type="binding site" evidence="1">
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            <position position="42"/>
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        <ligand>
            <name>isopentenyl diphosphate</name>
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        <ligand>
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        <ligand>
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        <ligand>
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            <label>1</label>
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        <ligand>
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            <label>2</label>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
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        <ligand>
            <name>Mg(2+)</name>
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            <label>1</label>
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    <feature type="binding site" evidence="1">
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        <ligand>
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    </feature>
    <feature type="mutagenesis site" description="Generates the same C30 product as the wild-type." evidence="4">
        <original>Y</original>
        <variation>A</variation>
        <location>
            <position position="124"/>
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    </feature>
    <feature type="mutagenesis site" description="Generates only one C5-prenyl unit longer C35 product." evidence="4">
        <original>L</original>
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            <position position="164"/>
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    <feature type="mutagenesis site" description="Generates the same C30 product as the wild-type." evidence="4">
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    <feature type="strand" evidence="9">
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        <source>
            <dbReference type="UniProtKB" id="P14324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q12051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="11790729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="16291686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000305" key="6">
        <source>
            <dbReference type="PubMed" id="11790729"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="2AZJ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="2AZK"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="2AZJ"/>
        </source>
    </evidence>
    <sequence length="281" mass="32275" checksum="AD14ABD58DE76CCB" modified="2001-10-01" version="1">MSIIEFWLEAKATIDRLIEQFLNSNRDWDLVDISSYILKDGKRFRGTLNMFFTVALGGDIKDSYGGALAIEILHSASLALDDIVDLDATRRGDKAAWVVYGNRKVIFITNYLIPTALRIIQTSYGDDALNTSIELWKDTSVGALRDMYDNSDYIRTIELKTGSLFKLSTVLSAYASKHYNTKQQMLDVGKYLGIIYQVIDDFVDYKTKKVEEIDGSAKQLFKYYREGKLEEYVRSVYLEYKQKYDELISNIPFQSKYLSEIRSLPEFLANGLLKEANIDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WC1</accession>
    <name>Q97WC1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">One of two inversely orientated ORFs in ISC1043</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90401">
        <property type="entry name" value="D90401"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WC1"/>
    <dbReference type="PaxDb" id="273057-SSO2311"/>
    <dbReference type="EnsemblBacteria" id="AAK42467">
        <property type="protein sequence ID" value="AAK42467"/>
        <property type="gene ID" value="SSO2311"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2311"/>
    <dbReference type="HOGENOM" id="CLU_1727318_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WC1"/>
    <dbReference type="PhylomeDB" id="Q97WC1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="152" mass="17271" checksum="99398C86C5FA3EFE" modified="2001-10-01" version="1">MDPLFLDLIYDPPCPPFNEDQSLSRLKYSSKYSSGFFMNFNIISLHLFPPLIVALGYQVDLRIREVIQYRLAIVLVSPQGVNLYTKIPQDLEGHLISLPLTLKRITVSDNQVTTQVKDEEGVHELLPSHLPQTSPFYSCNAWLLLGNSLSRP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-06-07" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WC6</accession>
    <name>CBID_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cobalt-precorrin-5B C(1)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.195</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cobalt-precorrin-6A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbiD</name>
        <name type="ordered locus">SSO2305</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylation of C-1 in cobalt-precorrin-5B to form cobalt-precorrin-6A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Co-precorrin-5B + S-adenosyl-L-methionine = Co-precorrin-6A + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:26285"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:60063"/>
            <dbReference type="ChEBI" id="CHEBI:60064"/>
            <dbReference type="EC" id="2.1.1.195"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 6/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CbiD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.195" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90400">
        <property type="entry name" value="G90400"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991807.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WC6"/>
    <dbReference type="SMR" id="Q97WC6"/>
    <dbReference type="FunCoup" id="Q97WC6">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2305"/>
    <dbReference type="PaxDb" id="273057-SSO2305"/>
    <dbReference type="EnsemblBacteria" id="AAK42462">
        <property type="protein sequence ID" value="AAK42462"/>
        <property type="gene ID" value="SSO2305"/>
    </dbReference>
    <dbReference type="GeneID" id="1453789"/>
    <dbReference type="GeneID" id="44128032"/>
    <dbReference type="KEGG" id="sso:SSO2305"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2394"/>
    <dbReference type="eggNOG" id="arCOG04383">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041273_1_0_2"/>
    <dbReference type="InParanoid" id="Q97WC6"/>
    <dbReference type="PhylomeDB" id="Q97WC6"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00227"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043780">
        <property type="term" value="F:cobalt-precorrin-5B C1-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2110.10">
        <property type="entry name" value="CbiD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10720">
        <property type="entry name" value="CbiD-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00787">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002748">
        <property type="entry name" value="CbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036074">
        <property type="entry name" value="CbiD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00312">
        <property type="entry name" value="cbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35863">
        <property type="entry name" value="COBALT-PRECORRIN-5B C(1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35863:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-5B C(1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01888">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026782">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111342">
        <property type="entry name" value="CbiD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000141699" description="Cobalt-precorrin-5B C(1)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00787"/>
        </source>
    </evidence>
    <sequence length="349" mass="37755" checksum="D6B69A811E157EE9" modified="2001-10-01" version="1">MIINSLKRFGITTGAAASAAAKAAVIGLLNKEKRSTVVIPTPIGLRLEIPVEKVEIDSEISCAEVKKFSGDNPDILDGLVIRCCAKLNENNEIVIIGERGVGKVTRSGLKATMGEMAISPTVREMIINAIREVTDKGIQITIEVPNGETIAEKTLNKMVGIVGGISILGTTGIETPVSDDDYLEHIRCELNVIRQSYDYVVIAPGNSAAKYAAELFDSNCIIKVGDRIGDSIKLASNLFKKVILAGLPAKLLKVYAGIFNTHYSQGDARLESLTHASVLAGLPYGTLTKISNALSVEEAFTYMTKEQRRKVMNIVAEKILSRIKSFNDNINFCVIIFDYDGESLSRVGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WD2</accession>
    <name>Q97WD2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thioesterase superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2006-03" db="PDB data bank">
            <title>Crystal structure of Conserved hypothetical protein (13815598) from Sulfolobus solfataricus at 1.91 A resolution.</title>
            <authorList>
                <consortium name="Joint Center for Structural Genomics (JCSG)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.91 ANGSTROMS) IN COMPLEX WITH COA</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 4-hydroxybenzoyl-CoA thioesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99400">
        <property type="entry name" value="A99400"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991816.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2GF6">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.91 A"/>
        <property type="chains" value="A/B/C/D=1-134"/>
    </dbReference>
    <dbReference type="PDBsum" id="2GF6"/>
    <dbReference type="AlphaFoldDB" id="Q97WD2"/>
    <dbReference type="SMR" id="Q97WD2"/>
    <dbReference type="STRING" id="273057.SSO2295"/>
    <dbReference type="PaxDb" id="273057-SSO2295"/>
    <dbReference type="EnsemblBacteria" id="AAK42456">
        <property type="protein sequence ID" value="AAK42456"/>
        <property type="gene ID" value="SSO2295"/>
    </dbReference>
    <dbReference type="GeneID" id="1453783"/>
    <dbReference type="KEGG" id="sso:SSO2295"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2388"/>
    <dbReference type="eggNOG" id="arCOG01137">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101141_3_2_2"/>
    <dbReference type="InParanoid" id="Q97WD2"/>
    <dbReference type="PhylomeDB" id="Q97WD2"/>
    <dbReference type="EvolutionaryTrace" id="Q97WD2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000165">
        <property type="entry name" value="Thioesterase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050563">
        <property type="entry name" value="4-hydroxybenzoyl-CoA_TE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793">
        <property type="entry name" value="4-HYDROXYBENZOYL-COA THIOESTERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793:SF27">
        <property type="entry name" value="NOVEL THIOESTERASE SUPERFAMILY DOMAIN AND SAPOSIN A-TYPE DOMAIN CONTAINING PROTEIN (0610012H03RIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13279">
        <property type="entry name" value="4HBT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="5" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="2GF6"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15673" checksum="CEEF7D924C969D8E" modified="2001-10-01" version="1">MENIEYVFEDVVRIYDTDAQGIAHYAAYYRFFTNTIEKFIKEKVGIPYPIVNENLWFVIAESHAIYHRPVKLGDKLTVLLNPKILSNKTIKFEFKVLKDGELTTEGYVIQIAINPKIWKSTEMPKEIMDKLSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="150" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WI4</accession>
    <name>Q97WI4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thioredoxin (TrxA-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">trxA-2</name>
        <name evidence="6" type="ordered locus">SSO2232</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2009" name="Proteins" volume="77" first="1004" last="1008">
            <title>The dimeric structure of Sulfolobus solfataricus thioredoxin A2 and the basis of its thermostability.</title>
            <authorList>
                <person name="Ruggiero A."/>
                <person name="Masullo M."/>
                <person name="Marasco D."/>
                <person name="Ruocco M.R."/>
                <person name="Grimaldi P."/>
                <person name="Arcari P."/>
                <person name="Zagari A."/>
                <person name="Vitagliano L."/>
            </authorList>
            <dbReference type="PubMed" id="19768676"/>
            <dbReference type="DOI" id="10.1002/prot.22559"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.83 ANGSTROMS) OF 26-135</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90393">
        <property type="entry name" value="C90393"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992040.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3HHV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.83 A"/>
        <property type="chains" value="A=26-135"/>
    </dbReference>
    <dbReference type="PDBsum" id="3HHV"/>
    <dbReference type="AlphaFoldDB" id="Q97WI4"/>
    <dbReference type="SMR" id="Q97WI4"/>
    <dbReference type="FunCoup" id="Q97WI4">
        <property type="interactions" value="262"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2232"/>
    <dbReference type="PaxDb" id="273057-SSO2232"/>
    <dbReference type="EnsemblBacteria" id="AAK42402">
        <property type="protein sequence ID" value="AAK42402"/>
        <property type="gene ID" value="SSO2232"/>
    </dbReference>
    <dbReference type="GeneID" id="1453729"/>
    <dbReference type="KEGG" id="sso:SSO2232"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2329"/>
    <dbReference type="eggNOG" id="arCOG01972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_10_1_2"/>
    <dbReference type="InParanoid" id="Q97WI4"/>
    <dbReference type="PhylomeDB" id="Q97WI4"/>
    <dbReference type="EvolutionaryTrace" id="Q97WI4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000598">
        <property type="entry name" value="Thioredoxin (TrxA-2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663:SF11">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="8" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="11"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="60"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="3HHV"/>
        </source>
    </evidence>
    <sequence length="135" mass="15520" checksum="FC593D294C48EB49" modified="2001-10-01" version="1">MNDELNDPELQKILSKKTTQILNNLKEKVKEPVKHLNSKNFDEFITKNKIVVVDFWAEWCAPCLILAPVIEELANDYPQVAFGKLNTEESQDIAMRYGIMSLPTIMFFKNGELVDQILGAVPREEIEVRLKSLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WQ0</accession>
    <name>Q97WQ0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetate--CoA ligase</fullName>
            <ecNumber evidence="2">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">acsA-7</name>
        <name evidence="9" type="ordered locus">SSO2070</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90374">
        <property type="entry name" value="E90374"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990190.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WQ0"/>
    <dbReference type="FunCoup" id="Q97WQ0">
        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2070"/>
    <dbReference type="PaxDb" id="273057-SSO2070"/>
    <dbReference type="EnsemblBacteria" id="AAK42252">
        <property type="protein sequence ID" value="AAK42252"/>
        <property type="gene ID" value="SSO2070"/>
    </dbReference>
    <dbReference type="GeneID" id="1453579"/>
    <dbReference type="KEGG" id="sso:SSO2070"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2147"/>
    <dbReference type="eggNOG" id="arCOG01529">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_2"/>
    <dbReference type="InParanoid" id="Q97WQ0"/>
    <dbReference type="PhylomeDB" id="Q97WQ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="8">
        <location>
            <begin position="34"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="6">
        <location>
            <begin position="100"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="7">
        <location>
            <begin position="500"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="608" mass="68504" checksum="DFEDAEBCD3397185" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WR2</accession>
    <name>Q97WR2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flavin reductase like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2055</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99373">
        <property type="entry name" value="A99373"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923760.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WR2"/>
    <dbReference type="SMR" id="Q97WR2"/>
    <dbReference type="STRING" id="273057.SSO2055"/>
    <dbReference type="PaxDb" id="273057-SSO2055"/>
    <dbReference type="EnsemblBacteria" id="AAK42240">
        <property type="protein sequence ID" value="AAK42240"/>
        <property type="gene ID" value="SSO2055"/>
    </dbReference>
    <dbReference type="GeneID" id="1453567"/>
    <dbReference type="KEGG" id="sso:SSO2055"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2135"/>
    <dbReference type="eggNOG" id="arCOG02017">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059021_1_2_2"/>
    <dbReference type="InParanoid" id="Q97WR2"/>
    <dbReference type="PhylomeDB" id="Q97WR2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042602">
        <property type="term" value="F:riboflavin reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050268">
        <property type="entry name" value="NADH-dep_flavin_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30466">
        <property type="entry name" value="FLAVIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30466:SF1">
        <property type="entry name" value="FMN REDUCTASE (NADH) RUTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin reductase like" evidence="3">
        <location>
            <begin position="9"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="155" mass="17808" checksum="A65A8EA67EDFFBF1" modified="2001-10-01" version="1">MSETIRNIMRLFPLGIVVVTTKWSDNLVGMTVNTFNSLSLNPPLVMFTADKTKGNDVPFIESNGFIVNFVDDEKIFNTFAFKPIKERFKEAKFFEGLDGIPVLADSYAYMEVKKYKTIDIGDHTIVVGEVINGKIVRDNFSPLVYYNRNYWKLCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WX8</accession>
    <name>Q97WX8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1983</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90364">
        <property type="entry name" value="F90364"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923710.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WX8"/>
    <dbReference type="STRING" id="273057.SSO1983"/>
    <dbReference type="PaxDb" id="273057-SSO1983"/>
    <dbReference type="EnsemblBacteria" id="AAK42173">
        <property type="protein sequence ID" value="AAK42173"/>
        <property type="gene ID" value="SSO1983"/>
    </dbReference>
    <dbReference type="GeneID" id="1453500"/>
    <dbReference type="KEGG" id="sso:SSO1983"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2058"/>
    <dbReference type="eggNOG" id="arCOG07852">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_427387_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WX8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="640" mass="71532" checksum="E05F21087273D515" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WZ2</accession>
    <name>Q97WZ2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1968</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90362">
        <property type="entry name" value="H90362"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991912.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WZ2"/>
    <dbReference type="SMR" id="Q97WZ2"/>
    <dbReference type="PaxDb" id="273057-SSO1968"/>
    <dbReference type="DNASU" id="1453486"/>
    <dbReference type="EnsemblBacteria" id="AAK42159">
        <property type="protein sequence ID" value="AAK42159"/>
        <property type="gene ID" value="SSO1968"/>
    </dbReference>
    <dbReference type="GeneID" id="1453486"/>
    <dbReference type="KEGG" id="sso:SSO1968"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2042"/>
    <dbReference type="eggNOG" id="arCOG00713">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125353_2_0_2"/>
    <dbReference type="InParanoid" id="Q97WZ2"/>
    <dbReference type="PhylomeDB" id="Q97WZ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09854">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052106">
        <property type="entry name" value="PINc/VapC_TA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38826">
        <property type="entry name" value="RIBONUCLEASE VAPC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38826:SF5">
        <property type="entry name" value="RIBONUCLEASE VAPC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="2"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="128" mass="15043" checksum="E69E38D1DE1820C6" modified="2001-10-01" version="1">MIFLDANFLIYLNSGVSEVKEYYIKLLTYESLFSDPLVIDEVIYVSKKKYGVKYCDTIEFLDEIVLKYLTVLPITIKEYERAKEIMRKYSVKPSDAFHIAVMLNNSINVILSEDKELDKVAEIKRIWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X61</accession>
    <name>Q97X61_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cobalt transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1892</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="D90353"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="Q97X61">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1892"/>
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    <dbReference type="EnsemblBacteria" id="AAK42083">
        <property type="protein sequence ID" value="AAK42083"/>
        <property type="gene ID" value="SSO1892"/>
    </dbReference>
    <dbReference type="GeneID" id="1453410"/>
    <dbReference type="KEGG" id="sso:SSO1892"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1948"/>
    <dbReference type="eggNOG" id="arCOG02250">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16914">
        <property type="entry name" value="EcfT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33514">
        <property type="entry name" value="PROTEIN ABCI12, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33514:SF13">
        <property type="entry name" value="PROTEIN ABCI12, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
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            <begin position="56"/>
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        <location>
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    </feature>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="35738" checksum="7122DB0086E68069" modified="2001-10-01" version="1">MNSLVDQIISWIIALYGSAVPALLVIFLIGITGLKEITRYETGNTFMYKLNPVTKVILTIVVMTVAATTVWWIGAILTLILLLSYLTLNEGVRKFGYVSFLTLSTILGGTWSVAPYTPPSTLQLAFPNEKQFTVVWTFPSYFQVMGYEQQLTLQALIYGLQTSFRIAPVLLSALLLITTTTGSDIFRMFTKIKIPIAITFSVLVGIRMIPRIFELLDTSVKMQYMRGLGYGKPKLISSLLIVYAIFEAIIPTMVYLFRGAKNLAISADTRGFRAYPSRTSLVELKFTRVDYYGVAIIIALIVMAILANLLGFGRTIPYVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XE4</accession>
    <name>Q97XE4_SACS2</name>
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    </protein>
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        <lineage>
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    </organism>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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            <end position="38"/>
        </location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="AAK41997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="140" mass="16369" checksum="21B88A31844DC30B" modified="2001-10-01" version="1">MLEVFVVFIDDPTFYYWLVLQSLINYISGTIVFVPIYFKLMQENYQRNLRIVALGWYGINTLLGIFGFLIYKYFYHQIVPLYVLFGITLSTIAFFAILGFMYSGISFDFFNNPHKLETKRVIIGMIISVIGIILTVILFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-01-24" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XE8</accession>
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            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
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                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <property type="gene ID" value="SSO8936"/>
    </dbReference>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2534836_0_0_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="AAK41993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="83" mass="9569" checksum="001A86C0D1725C12" modified="2001-10-01" version="1">MEGEIRDSKRFMDFLGGKIPPKSTVFSFYKKLQQTVIQEGETMRTTLMDELNKALDKAISEYREKGFELEVGREKTIGSRTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XF0</accession>
    <name>Q97XF0_SACS2</name>
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        <submittedName>
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        </submittedName>
    </protein>
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    <organism evidence="1 2">
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            <taxon>Thermoproteota</taxon>
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            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
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                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
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        <property type="entry name" value="H90341"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XF0"/>
    <dbReference type="STRING" id="273057.SSO1799"/>
    <dbReference type="PaxDb" id="273057-SSO1799"/>
    <dbReference type="EnsemblBacteria" id="AAK41991">
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        <property type="gene ID" value="SSO1799"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1799"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1851"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2044519_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XF0"/>
    <dbReference type="PhylomeDB" id="Q97XF0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="120" mass="14429" checksum="152522A8248EEB35" modified="2001-10-01" version="1">MLWKEKDIRGKKTDFYDAEKLENMVNNAREYVNNPLKELVSLYLFMKDVQTKQENRLRRALFLVSDEEKLSEERLEKFSKGDFSDVKLYQLEYTGTVLREIQVLARTLLQTRDVVRFEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XH1</accession>
    <name>Q97XH1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90338">
        <property type="entry name" value="D90338"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XH1"/>
    <dbReference type="STRING" id="273057.SSO1768"/>
    <dbReference type="PaxDb" id="273057-SSO1768"/>
    <dbReference type="EnsemblBacteria" id="AAK41963">
        <property type="protein sequence ID" value="AAK41963"/>
        <property type="gene ID" value="SSO1768"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1768"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1819"/>
    <dbReference type="eggNOG" id="arCOG07189">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_738931_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XH1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="41824" checksum="2136BD01FEE17E0F" modified="2001-10-01" version="1">MVIQKMKRTEREIYFLLVLVSIASSQQFYIPVYFERIYGDAELAFILFTIAVALRAFLAPFIAQILKNFIKLNIIVAFSFQSLFYILLYLKIPINFKLAFILGAFYGIASSLILFLNAVYFSYSEPTFSSRYKSANFFISSKFIGYTIGSTISAIILFFYNLADILIIASIFWLSSIPLVFTLNVEKTEVKKPSLGKVKESLFLVPLPLLISFVFSMSLASVGGIIEPLIFNKINAGWFSISFILANIASSLVPIYFPKNKIKLSLALGTFLAVITEFLFPLTPFLYLLIIYGILSQVGNSLVWPVAIGKIVSKSKFPVVESGVLITLTDISSLIADGYVSLSFPLINVLSLSLFSIIDLFSWLPLLLSKIDVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XJ3</accession>
    <name>Q97XJ3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Terminal quinol oxidase, subunit (DoxD)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">doxD</name>
        <name evidence="3" type="ordered locus">SSO1742</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90335">
        <property type="entry name" value="D90335"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XJ3"/>
    <dbReference type="STRING" id="273057.SSO1742"/>
    <dbReference type="PaxDb" id="273057-SSO1742"/>
    <dbReference type="EnsemblBacteria" id="AAK41939">
        <property type="protein sequence ID" value="AAK41939"/>
        <property type="gene ID" value="SSO1742"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1742"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1787"/>
    <dbReference type="eggNOG" id="arCOG02862">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1544122_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XJ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007301">
        <property type="entry name" value="DoxD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053663">
        <property type="entry name" value="TQO_large_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041179">
        <property type="entry name" value="TQO_large_DoxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04173">
        <property type="entry name" value="DoxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="TQO small subunit DoxD" evidence="2">
        <location>
            <begin position="18"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="183" mass="20524" checksum="4D3305BD68743ED1" modified="2001-10-01" version="1">MGKMANKQVVDNLTRKEYLFPIRFAVGWMFFDGGIRKAILKPAKLDPNSSSFVGGKLVNFLPHAGPFKDFLLMTLENRTFDVTFLTIFSYVEILVGLFLIIGFLTRLSAFGAAVMAIGMAPAYWLGSTCEDEWQIGSLLTAGAIVLMLTAAGRVWGVDYYLYKKFGDWSLSKKIPLLNLIKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XL0</accession>
    <name>Q97XL0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Second ORF in transposon ISC1491</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90333">
        <property type="entry name" value="C90333"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XL0"/>
    <dbReference type="STRING" id="273057.SSO1720"/>
    <dbReference type="PaxDb" id="273057-SSO1720"/>
    <dbReference type="EnsemblBacteria" id="AAK41922">
        <property type="protein sequence ID" value="AAK41922"/>
        <property type="gene ID" value="SSO1720"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1720"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1766"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098916_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XL0"/>
    <dbReference type="PhylomeDB" id="Q97XL0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="152" mass="17521" checksum="337CFECD3CD530E1" modified="2001-10-01" version="1">MKEVFGKKTDKLDAQRLAKAFILGAVKGSYIPTGEIRELRELTRYRESLMRKITQVKNEIRKFLEMAGYKIEPFDRRGMALLEKLSRGEGLSKEERDELKEKLGRSLNDAEKLALKQLVDLLKSLEAMVKEVEDMIISKIPQPVVELSRELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XM3</accession>
    <name>Q97XM3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Plasmid stabilization system</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90330">
        <property type="entry name" value="E90330"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990201.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XM3"/>
    <dbReference type="STRING" id="273057.SSO1690"/>
    <dbReference type="PaxDb" id="273057-SSO1690"/>
    <dbReference type="EnsemblBacteria" id="AAK41900">
        <property type="protein sequence ID" value="AAK41900"/>
        <property type="gene ID" value="SSO1690"/>
    </dbReference>
    <dbReference type="GeneID" id="1454679"/>
    <dbReference type="KEGG" id="sso:SSO1690"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1742"/>
    <dbReference type="eggNOG" id="arCOG07307">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2340317_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XM3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="11488" checksum="542DAFA171421F49" modified="2001-10-01" version="1">MLTLSAHLRKHLEDINNYLKKFNNTIDPLSDNVLSFLANLKGTPQVPNKILGESERWRVSFFILNPVQKIRYVIAKRGEELILVTAHPDPDADKFVEFQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XM8</accession>
    <name>Q97XM8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial transposase ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO8687</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90329">
        <property type="entry name" value="H90329"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923564.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XM8"/>
    <dbReference type="PaxDb" id="273057-SSO8687"/>
    <dbReference type="EnsemblBacteria" id="AAK41895">
        <property type="protein sequence ID" value="AAK41895"/>
        <property type="gene ID" value="SSO8687"/>
    </dbReference>
    <dbReference type="GeneID" id="1454674"/>
    <dbReference type="KEGG" id="sso:SSO8687"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1735"/>
    <dbReference type="eggNOG" id="arCOG03169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2461932_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XM8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048907">
        <property type="entry name" value="WHD_MCM_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21100">
        <property type="entry name" value="WHD_MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal" evidence="1">
        <location>
            <begin position="10"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="88" mass="10466" checksum="FFFEC9DA6554CDEA" modified="2001-10-01" version="1">MNSQWITESLKAIATLENARWKNIMDYVIAKTERKISNTTLSRDLRKLMKMGFIEKENDEYKNPRSNYEVHIAKRLSLIFNILYSILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XQ7</accession>
    <name>Q97XQ7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helicase of the snf2/rad54 family (Amino end), hypothetical</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1653</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4 5 6" key="2">
        <citation type="journal article" date="2005" name="Cell" volume="121" first="363" last="373">
            <title>X-ray structures of the Sulfolobus solfataricus SWI2/SNF2 ATPase core and its complex with DNA.</title>
            <authorList>
                <person name="Durr H."/>
                <person name="Korner C."/>
                <person name="Muller M."/>
                <person name="Hickmann V."/>
                <person name="Hopfner K.P."/>
            </authorList>
            <dbReference type="PubMed" id="15882619"/>
            <dbReference type="DOI" id="10.1016/j.cell.2005.03.026"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 659-789</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90326">
        <property type="entry name" value="C90326"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923549.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1Z5Z">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B=659-789"/>
    </dbReference>
    <dbReference type="PDB" id="1Z63">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="A/B=430-496, A/B=498-789"/>
    </dbReference>
    <dbReference type="PDB" id="1Z6A">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="A=431-789"/>
    </dbReference>
    <dbReference type="PDBsum" id="1Z5Z"/>
    <dbReference type="PDBsum" id="1Z63"/>
    <dbReference type="PDBsum" id="1Z6A"/>
    <dbReference type="AlphaFoldDB" id="Q97XQ7"/>
    <dbReference type="SMR" id="Q97XQ7"/>
    <dbReference type="STRING" id="273057.SSO1653"/>
    <dbReference type="PaxDb" id="273057-SSO1653"/>
    <dbReference type="EnsemblBacteria" id="AAK41866">
        <property type="protein sequence ID" value="AAK41866"/>
        <property type="gene ID" value="SSO1653"/>
    </dbReference>
    <dbReference type="GeneID" id="25392519"/>
    <dbReference type="KEGG" id="sso:SSO1653"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1702"/>
    <dbReference type="eggNOG" id="arCOG00883">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_350795_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XQ7"/>
    <dbReference type="PhylomeDB" id="Q97XQ7"/>
    <dbReference type="BRENDA" id="3.6.4.B9">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97XQ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140750">
        <property type="term" value="F:nucleosome array spacer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18012">
        <property type="entry name" value="DEXQc_arch_SWI2_SNF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000091">
        <property type="entry name" value="DNA helicase, SNF2/RAD54 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022138">
        <property type="entry name" value="DUF3670"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12419">
        <property type="entry name" value="DUF3670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4 5 6" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="456"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="1Z5Z"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="1Z63"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="1Z6A"/>
        </source>
    </evidence>
    <sequence length="802" mass="92853" checksum="80F86655C9F5040A" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XR2</accession>
    <name>Q97XR2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Second ORF in transposon ISC1250</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1648</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90325">
        <property type="entry name" value="F90325"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XR2"/>
    <dbReference type="PaxDb" id="273057-SSO1648"/>
    <dbReference type="EnsemblBacteria" id="AAK41861">
        <property type="protein sequence ID" value="AAK41861"/>
        <property type="gene ID" value="SSO1648"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1648"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1696"/>
    <dbReference type="eggNOG" id="arCOG04918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124269_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XR2"/>
    <dbReference type="PhylomeDB" id="Q97XR2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217:SF7">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00872">
        <property type="entry name" value="Transposase_mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="157" mass="17592" checksum="DE32C7A9FA980D79" modified="2001-10-01" version="1">MLSVLGGKLSAKLWMPEIQASGNFKYVIVDGKYVKLKGGKGVLLSAIGATPEGKRAVLDITLSVEKDVMSYWRFLVRVWKKYSFVLVVVDGTKALDKISLAELQVRRQGCLVHLKRNASKEEREALKIISSTESGEIKHETCPRLLSYLSVPKELWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XT0</accession>
    <name>Q97XT0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Partial transposase ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1627</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90323">
        <property type="entry name" value="C90323"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XT0"/>
    <dbReference type="PaxDb" id="273057-SSO1627"/>
    <dbReference type="EnsemblBacteria" id="AAK41842">
        <property type="protein sequence ID" value="AAK41842"/>
        <property type="gene ID" value="SSO1627"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1627"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1671"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074024_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XT0"/>
    <dbReference type="PhylomeDB" id="Q97XT0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025471">
        <property type="entry name" value="DUF4322"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033541">
        <property type="entry name" value="transpos_ISH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14210">
        <property type="entry name" value="DUF4322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4322" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="324" mass="37724" checksum="0E4847B222D99FC7" modified="2001-10-01" version="1">MLNFQGEKVDKVAKTLISACLWNDSVENKSRAYDVSPQTVRNYVEKQGMEVIEKLLERARKISLEILKGVKEIDLSIDWTTKTWYGKPVKGSSEKGNSWNYATTKYKGKVLLLAFIPQVNGMTKDEIVKVLVEQVMAMGFKIRLITLDAGFYTVDVLNFISQFKYIIAVLVGDVKVYEEFDGDYTTNSKRHRRDEQVKFRFLVYSKEEVRRKSLVYFARATNLDLSKREVLDLYNKVRGPIETSYRNIKAFLPFTGSTKFVFRTLIFVLALVLYSLYTIFKGEVGREEFRLLLILLFPDLFNPENFTFNAIETLIYTIDLFLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XU2</accession>
    <name>Q97XU2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90321">
        <property type="entry name" value="F90321"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XU2"/>
    <dbReference type="STRING" id="273057.SSO1614"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1614"/>
    <dbReference type="EnsemblBacteria" id="AAK41829">
        <property type="protein sequence ID" value="AAK41829"/>
        <property type="gene ID" value="SSO1614"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1614"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1656"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_812862_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XU2"/>
    <dbReference type="PhylomeDB" id="Q97XU2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="341" mass="39599" checksum="1C8E2753339CF147" modified="2001-10-01" version="1">MPCLLLKGDIIESKTGTVRWALEVQKAIPDLSLFYFGKSNSLQKNQYLIKIAKIGSIYIILPTFSNLRNIAKLLKCNSVYFVDIPSLPFFLLIYFILKLFGRKIIFGLHGFIQRDNGIKGKIFRNVAKHETVHVLNVYDKNLMEKIGCKRIFLIPNFIYSNSSEIIRCNKFIVLFVGRLDINHKGIDLLVDIILKLKTNIEFHIIGNGEGKNVIEELAKKQTNVKYLGEVNDEVLSQEYARASLFILTSRYETFGYVLLEAQSHGLPIVAFDLPEIRDNIILGKLVRPFNTEDFARAILDFYNQYKLNKEKYYLIREEIRRKAYDIFNKQKIIDNILKLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XU6</accession>
    <name>Q97XU6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SSV1 hypothetical 14.8 kd protein (Orf B-129) homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO8481</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99321">
        <property type="entry name" value="A99321"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923513.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XU6"/>
    <dbReference type="STRING" id="273057.SSO8481"/>
    <dbReference type="PaxDb" id="273057-SSO8481"/>
    <dbReference type="EnsemblBacteria" id="AAK41824">
        <property type="protein sequence ID" value="AAK41824"/>
        <property type="gene ID" value="SSO8481"/>
    </dbReference>
    <dbReference type="GeneID" id="34442709"/>
    <dbReference type="KEGG" id="sso:SSO8481"/>
    <dbReference type="eggNOG" id="arCOG06947">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_189640_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XU6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054177">
        <property type="entry name" value="B129_C2H2-Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22034">
        <property type="entry name" value="B129_C2H2-zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="63" mass="7205" checksum="44469119D10F9321" modified="2001-10-01" version="1">MGIIGPYVCPLCLMPFNSSVSLKQHIRYTEHTKTCPICKKEFRNTDSTLDHVCKKHNISALVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y21</accession>
    <name>Q97Y21_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acetoin catabolism protein X homolog (AcoX)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">acoX</name>
        <name evidence="1" type="ordered locus">SSO1527</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90311">
        <property type="entry name" value="G90311"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923453.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y21"/>
    <dbReference type="STRING" id="273057.SSO1527"/>
    <dbReference type="PaxDb" id="273057-SSO1527"/>
    <dbReference type="EnsemblBacteria" id="AAK41750">
        <property type="protein sequence ID" value="AAK41750"/>
        <property type="gene ID" value="SSO1527"/>
    </dbReference>
    <dbReference type="GeneID" id="1454529"/>
    <dbReference type="KEGG" id="sso:SSO1527"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1567"/>
    <dbReference type="eggNOG" id="arCOG01350">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_786821_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y21"/>
    <dbReference type="PhylomeDB" id="Q97Y21"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039065">
        <property type="entry name" value="AcoX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011391">
        <property type="entry name" value="AcoX_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40697">
        <property type="entry name" value="ACETOIN CATABOLISM PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40697:SF3">
        <property type="entry name" value="ACETOIN CATABOLISM PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018567">
        <property type="entry name" value="AcoX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="339" mass="37376" checksum="D0837B59A5B60C6B" modified="2001-10-01" version="1">MPAVGIIVNPESGRDIRRLVAKATFVSNYAKIDTIKRFLYGLNITNSVDEVVFMPDFYGISLDIISDIKDDVKFKLSTLDMRVENTADDTINASKYMIESGVDVIVSAGGDGTLRAVYKGAGDKVPILGLSLGTNNVLGALYEPTVLGMMLGFLLKENKAISNVVERIKTIKLFVNNEFKNLALVDLTFMSGWYVGARAIWDEYSLRYAFISKGELGDIGIPSIASLIRPITFQDDLGLMVKFGLGKIKVAATLAPGLVKEVFIDGISILKLGQEVHLPSGSYVIAFDGEKEMVVNSKDEAMVRIDRDGPLLVSIPKSLNYINSYYREEMGELRWAKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y23</accession>
    <name>Q97Y23_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="4">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pdhA-2</name>
        <name evidence="9" type="ordered locus">SSO1525</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90311">
        <property type="entry name" value="E90311"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923451.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y23"/>
    <dbReference type="FunCoup" id="Q97Y23">
        <property type="interactions" value="187"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1525"/>
    <dbReference type="PaxDb" id="273057-SSO1525"/>
    <dbReference type="EnsemblBacteria" id="AAK41748">
        <property type="protein sequence ID" value="AAK41748"/>
        <property type="gene ID" value="SSO1525"/>
    </dbReference>
    <dbReference type="GeneID" id="1454527"/>
    <dbReference type="KEGG" id="sso:SSO1525"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1565"/>
    <dbReference type="eggNOG" id="arCOG01054">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029393_5_0_2"/>
    <dbReference type="InParanoid" id="Q97Y23"/>
    <dbReference type="PhylomeDB" id="Q97Y23"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000013">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="8">
        <location>
            <begin position="16"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="332" mass="36691" checksum="9077D2A2B4F9029B" modified="2001-10-01" version="1">MFQDIPKSKLLDMYKKMLLIRYHELTAKELFASGKIPGFVHLYVGEEAVAVGVMSTLRDDDYITSTHRGHGHCIAKGLDVKRMLAEIMGKKTGVCKGKGGSMHIFDYSKGMLGANGIVGGGAPHAVGAALAFKLKGLDRVAVAFIGDGAMNQGVVLESLNLSAIWKLPVIFVVEDNMYAMSTRSLVPGKLQPRHSAAKSYVERALGFGIPAVEVDGMDVLAVYEVAKEAVDRARRGGGPSLLHCKTYRFFGHFEGDPLVYRDKEEEEMWRKRDPITLFRDKLVSNNIINSEELDKIDREAKAEIEQALKFAEESPYPEVEEALTDVFTDNSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y70</accession>
    <name>Q97Y70_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1472</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90305">
        <property type="entry name" value="D90305"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y70"/>
    <dbReference type="PaxDb" id="273057-SSO1472"/>
    <dbReference type="EnsemblBacteria" id="AAK41699">
        <property type="protein sequence ID" value="AAK41699"/>
        <property type="gene ID" value="SSO1472"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1472"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1510"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1943954_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y70"/>
    <dbReference type="PhylomeDB" id="Q97Y70"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="129" mass="14535" checksum="2EBDB80E4F66788A" modified="2001-10-01" version="1">MQTMILPKTELKSLAITLATNNINVISQDLDPEIVKAAPSLLTGNRGKYYLKVVRRGEKVISKGQRTFKFYPIYREVKGEINVVAVDETGLTVGEKEQEKAEGFLLYNLEEKRSKDEILGPRISLKVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y86</accession>
    <name>Q97Y86_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">CRISPR-associated exonuclease Cas4</fullName>
            <ecNumber evidence="3 13">3.1.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">SSO1449</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="13">Mg(2+) or Mn(2+) required for ssDNA cleavage activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the CRISPR-associated exonuclease Cas4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.12.1" evidence="3 13"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99303">
        <property type="entry name" value="A99303"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990819.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y86"/>
    <dbReference type="STRING" id="273057.SSO1449"/>
    <dbReference type="PaxDb" id="273057-SSO1449"/>
    <dbReference type="EnsemblBacteria" id="AAK41680">
        <property type="protein sequence ID" value="AAK41680"/>
        <property type="gene ID" value="SSO1449"/>
    </dbReference>
    <dbReference type="GeneID" id="1454459"/>
    <dbReference type="KEGG" id="sso:SSO1449"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1478"/>
    <dbReference type="eggNOG" id="arCOG00786">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126922_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y86"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045145">
        <property type="term" value="F:single-stranded DNA 5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051827">
        <property type="entry name" value="Cas4_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013343">
        <property type="entry name" value="CRISPR-assoc_prot_Cas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022765">
        <property type="entry name" value="Dna2/Cas4_DUF83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00372">
        <property type="entry name" value="cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531">
        <property type="entry name" value="CRISPR-ASSOCIATED EXONUCLEASE CAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531:SF2">
        <property type="entry name" value="CRISPR-ASSOCIATED EXONUCLEASE CAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01930">
        <property type="entry name" value="Cas_Cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="8 13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF83" evidence="14">
        <location>
            <begin position="5"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAK41680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="175" mass="20532" checksum="405F122F659CBE33" modified="2001-10-01" version="1">MVSVTDLKDYSLCKVIPWIRKKMGWKEPITNSQRIAKNVNLKEIVRDFPEPKYYEVHLRDKDSGLNGIVDVLTEDSVIEIKAFYRRFYNHFRIQLLAYAYLAERNGFRVRNTVLLMNKERKINIEVRKEHVEYIGKVVNELVESLEDDSPPVVNPSPLLCKACQYRRVCSVSVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y97</accession>
    <name>Q97Y97_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transposase ISC1173</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1436</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Absolutely required for transposition of IS1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase 27 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90301">
        <property type="entry name" value="F90301"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y97"/>
    <dbReference type="STRING" id="273057.SSO1436"/>
    <dbReference type="PaxDb" id="273057-SSO1436"/>
    <dbReference type="EnsemblBacteria" id="AAK41669">
        <property type="protein sequence ID" value="AAK41669"/>
        <property type="gene ID" value="SSO1436"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1436"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1465"/>
    <dbReference type="eggNOG" id="arCOG02133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076276_0_1_2"/>
    <dbReference type="InParanoid" id="Q97Y97"/>
    <dbReference type="PhylomeDB" id="Q97Y97"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005063">
        <property type="entry name" value="Transposase_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051354">
        <property type="entry name" value="Transposase_27_IS1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033558">
        <property type="entry name" value="transpos_IS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293:SF1">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03400">
        <property type="entry name" value="DDE_Tnp_IS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="35514" checksum="AC938B9800408CCE" modified="2001-10-01" version="1">MRVTNMDLVTLAQLILMILRNLNLKPRKYELKDIALALAAYSLGVQITKTGIPPSTLYYYLRKVGIRRRGESRPTCPSCNSNRVVKNGSSRGRAKYKCKACGRTFYDALKHRMDREQRERILKEYLNRMSMRAISRVEGKPLTTVYSLVRRIGIRAFTYLTILKGELKNFTAKSTVFDEFWTYFRVRHGVMREDLWIWTALADGVPFYEFGDRSYGTFRYLLGWLPRSEVNYTDYYCVYQVLDNRVASKKYTYLVESHNSWCRSHLARLARDTKAVTRSGRMVEYSLALLNVMYPHVFSKEIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YA4</accession>
    <name>Q97YA4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2023" name="Curr. Res. Struct. Biol." volume="5" first="100098" last="100098">
            <title>Structure of the &lt;i&gt;Saccharolobus solfataricus&lt;/i&gt; type III-D CRISPR effector.</title>
            <authorList>
                <person name="Cannone G."/>
                <person name="Kompaniiets D."/>
                <person name="Graham S."/>
                <person name="White M.F."/>
                <person name="Spagnolo L."/>
            </authorList>
            <dbReference type="PubMed" id="36843655"/>
            <dbReference type="DOI" id="10.1016/j.crstbi.2023.100098"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (3.50 ANGSTROMS) OF 7-539</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90300">
        <property type="entry name" value="G90300"/>
    </dbReference>
    <dbReference type="PDB" id="8BMW">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="K=7-539"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YA4"/>
    <dbReference type="SMR" id="Q97YA4"/>
    <dbReference type="STRING" id="273057.SSO1428"/>
    <dbReference type="PaxDb" id="273057-SSO1428"/>
    <dbReference type="EnsemblBacteria" id="AAK41662">
        <property type="protein sequence ID" value="AAK41662"/>
        <property type="gene ID" value="SSO1428"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1428"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1455"/>
    <dbReference type="eggNOG" id="arCOG02666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_488034_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YA4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052117">
        <property type="entry name" value="Cas10/Csm1_subtype-III-A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36528">
        <property type="entry name" value="CRISPR SYSTEM SINGLE-STRAND-SPECIFIC DEOXYRIBONUCLEASE CAS10/CSM1 (SUBTYPE III-A)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36528:SF1">
        <property type="entry name" value="CRISPR SYSTEM SINGLE-STRAND-SPECIFIC DEOXYRIBONUCLEASE CAS10_CSM1 (SUBTYPE III-A)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="165"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="8BMW"/>
        </source>
    </evidence>
    <sequence length="558" mass="64116" checksum="1F9908E4B1D48604" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YF5</accession>
    <name>Q97YF5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="4">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pdhB-1</name>
        <name evidence="9" type="ordered locus">SSO1370</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90293">
        <property type="entry name" value="E90293"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992546.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YF5"/>
    <dbReference type="FunCoup" id="Q97YF5">
        <property type="interactions" value="237"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1370"/>
    <dbReference type="PaxDb" id="273057-SSO1370"/>
    <dbReference type="EnsemblBacteria" id="AAK41604">
        <property type="protein sequence ID" value="AAK41604"/>
        <property type="gene ID" value="SSO1370"/>
    </dbReference>
    <dbReference type="GeneID" id="1454383"/>
    <dbReference type="KEGG" id="sso:SSO1370"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1375"/>
    <dbReference type="eggNOG" id="arCOG01052">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012907_1_0_2"/>
    <dbReference type="InParanoid" id="Q97YF5"/>
    <dbReference type="PhylomeDB" id="Q97YF5"/>
    <dbReference type="BRENDA" id="2.3.1.190">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43257">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43257:SF2">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="8">
        <location>
            <begin position="4"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="332" mass="36565" checksum="E085CB2C00765855" modified="2001-10-01" version="1">MKIRGIAQAIAEGIRQEMERNDRIVVLGEDVTYWGAVFGFTMGLFDKFGRKRVIDTPITEQTFMGISVGAASSGLHPVVSLMFVDFLGAGFDQMFNHMAKNYYMSGGQYPMPITVITAIGGGYGDSSQHSQVLYSLFAHLPGFKVIVPSTPYDAKGLTIKALRDNNPVIIFGHKLLTGLPFLPFEGNEEEVPEEPYEIEFGKAAIRKEGTDLTIISAGLMVHRSLKAAEMLQKEGISAEVIDVRTFVPLDEETIIKSARKTGRVLIVDEDYMSYGVTGEIAFRIQSKALKDLKVPISRLAVPDVPIPFSEPLENAVIPNVNTIYSEAKKLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YH8</accession>
    <name>Q97YH8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn-dependent hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1345</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90290">
        <property type="entry name" value="F90290"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923338.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YH8"/>
    <dbReference type="SMR" id="Q97YH8"/>
    <dbReference type="STRING" id="273057.SSO1345"/>
    <dbReference type="PaxDb" id="273057-SSO1345"/>
    <dbReference type="EnsemblBacteria" id="AAK41581">
        <property type="protein sequence ID" value="AAK41581"/>
        <property type="gene ID" value="SSO1345"/>
    </dbReference>
    <dbReference type="GeneID" id="1454360"/>
    <dbReference type="KEGG" id="sso:SSO1345"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1350"/>
    <dbReference type="eggNOG" id="arCOG00511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1080181_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YH8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051013">
        <property type="entry name" value="MBL_superfamily_lactonases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978:SF2">
        <property type="entry name" value="102 KBASES UNSTABLE REGION: FROM 1 TO 119443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978">
        <property type="entry name" value="QUORUM-QUENCHING LACTONASE YTNP-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="30583" checksum="961B8809AD34EA99" modified="2001-10-01" version="1">MVSLFKIIQVGDHGEVPGAEVFWMKDFDKWYRLSFYSFLIKTENDQYVLINTGLPDDLSLRNKFLKEWAKSDRCKFQHVEKIENALAKLNITTDDISHIIITPVQDYTIGRIHMFRKAKLYFSKKGWYEDVVTPSPSPFLNRDIYLPRLTREYLFDEAWNRIVLVENQEVIKGIEVKWTGCHHRSSMLVKFNFDKKRWCISDSAFVMNNFEQNIPIGIAEDIYECLNAYDYMRRECDVVIPSYDPENVNRFKEYILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YI8</accession>
    <name>Q97YI8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">isocitrate lyase</fullName>
            <ecNumber evidence="2">4.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">aceA/icl</name>
        <name evidence="9" type="ordered locus">SSO1333</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Can also use Mn(2+) ion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.1" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90289">
        <property type="entry name" value="D90289"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923328.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YI8"/>
    <dbReference type="FunCoup" id="Q97YI8">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1333"/>
    <dbReference type="PaxDb" id="273057-SSO1333"/>
    <dbReference type="EnsemblBacteria" id="AAK41571">
        <property type="protein sequence ID" value="AAK41571"/>
        <property type="gene ID" value="SSO1333"/>
    </dbReference>
    <dbReference type="GeneID" id="1454350"/>
    <dbReference type="KEGG" id="sso:SSO1333"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1339"/>
    <dbReference type="eggNOG" id="arCOG00583">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019214_2_0_2"/>
    <dbReference type="InParanoid" id="Q97YI8"/>
    <dbReference type="PhylomeDB" id="Q97YI8"/>
    <dbReference type="BRENDA" id="4.1.3.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004451">
        <property type="term" value="F:isocitrate lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000005">
        <property type="entry name" value="Isocitrate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006254">
        <property type="entry name" value="Isocitrate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018523">
        <property type="entry name" value="Isocitrate_lyase_ph_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01346">
        <property type="entry name" value="isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011645">
        <property type="entry name" value="PRK15063.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21631:SF3">
        <property type="entry name" value="BIFUNCTIONAL GLYOXYLATE CYCLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21631">
        <property type="entry name" value="ISOCITRATE LYASE/MALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00463">
        <property type="entry name" value="ICL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001362">
        <property type="entry name" value="Isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00161">
        <property type="entry name" value="ISOCITRATE_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="5 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="92"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="192"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="313"/>
            <end position="317"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="437" mass="48919" checksum="84D0E69DFE0473B5" modified="2001-10-01" version="1">MNIRDKWIEEEKSLEYEWSEDPRWRGIKRNYKPLDVVKLRGSIRIEYSLAKLASHKLWNLLNTEPYVATFGALTGSQAVEMAKAGLKAIYVSGWQVAADNNLSNQTYPDLSLYPSNSVPNLVKRLNNALIRADQISWSEGKHDIDYLLPIVADAEAGFGGPIHAFELTKALIEAGAAGVHFEDQLAAEKKCGHLGGKVLIPISAFIRVLNAARLASDVLGVPTILIARTDALNAKYLSNDVDETDNQFLTGKRTSEGYYEIKGGIEYAIARGLAYAPYADLLWFETSKPDLEEARQFAEAIHTHYPGKMLAYNLSPSFNWKKFMDDSKISKFMNELGEMGYKFQFITLAGWHLINYHTFKLARAYRNEGMPAYVRLQELEFQAQAEGYTAVSHQREVGTDYFDLVLTIASGGEASTTAMEGSTEAEQFVEAKQKVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YM5</accession>
    <name>Q97YM5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1298</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90284">
        <property type="entry name" value="H90284"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YM5"/>
    <dbReference type="STRING" id="273057.SSO1298"/>
    <dbReference type="PaxDb" id="273057-SSO1298"/>
    <dbReference type="EnsemblBacteria" id="AAK41535">
        <property type="protein sequence ID" value="AAK41535"/>
        <property type="gene ID" value="SSO1298"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1298"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1298"/>
    <dbReference type="HOGENOM" id="CLU_1754797_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YM5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="151" mass="17598" checksum="0FDE6FDD44013FF2" modified="2001-10-01" version="1">MGKMDKILVSKNELIEDDLINDYLMEMVKKLDQIVISARKNVEINGIKIPFYLDLEREIRLIFIYEESTLTYLNFHIENIKKIKELRRKDVKIILLINDISQLDLNGVVNLCENKTIPNVSITIGEAKSVSACKELEIHGYQGWILKDFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YQ1</accession>
    <name>Q97YQ1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Solute-binding protein family 5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1273</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90281">
        <property type="entry name" value="G90281"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923279.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YQ1"/>
    <dbReference type="FunCoup" id="Q97YQ1">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1273"/>
    <dbReference type="PaxDb" id="273057-SSO1273"/>
    <dbReference type="EnsemblBacteria" id="AAK41510">
        <property type="protein sequence ID" value="AAK41510"/>
        <property type="gene ID" value="SSO1273"/>
    </dbReference>
    <dbReference type="GeneID" id="1454289"/>
    <dbReference type="KEGG" id="sso:SSO1273"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1270"/>
    <dbReference type="eggNOG" id="arCOG01672">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010991_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YQ1"/>
    <dbReference type="PhylomeDB" id="Q97YQ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290:SF9">
        <property type="entry name" value="OLIGOPEPTIDE-BINDING PROTEIN APPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="882"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="domain" description="Solute-binding protein family 5" evidence="5">
        <location>
            <begin position="44"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="906" mass="97958" checksum="29371EDF382FE201" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YR5</accession>
    <name>Q97YR5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Citryl-CoA lyase beta subunit homolog (CitE)</fullName>
            <ecNumber evidence="5">4.1.3.34</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">citE</name>
        <name evidence="5" type="ordered locus">SSO1254</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="4.1.3.34" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90279">
        <property type="entry name" value="F90279"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991883.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YR5"/>
    <dbReference type="FunCoup" id="Q97YR5">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1254"/>
    <dbReference type="PaxDb" id="273057-SSO1254"/>
    <dbReference type="EnsemblBacteria" id="AAK41493">
        <property type="protein sequence ID" value="AAK41493"/>
        <property type="gene ID" value="SSO1254"/>
    </dbReference>
    <dbReference type="GeneID" id="1454272"/>
    <dbReference type="KEGG" id="sso:SSO1254"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1249"/>
    <dbReference type="eggNOG" id="arCOG00760">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044864_0_1_2"/>
    <dbReference type="InParanoid" id="Q97YR5"/>
    <dbReference type="PhylomeDB" id="Q97YR5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008816">
        <property type="term" value="F:citryl-CoA lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000071">
        <property type="entry name" value="Citryl-CoA lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011206">
        <property type="entry name" value="Citrate_lyase_beta/mcl1/mcl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32308:SF0">
        <property type="entry name" value="HPCH_HPAI ALDOLASE_CITRATE LYASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32308">
        <property type="entry name" value="LYASE BETA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G13030)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015582">
        <property type="entry name" value="Cit_lyase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpcH/HpaI aldolase/citrate lyase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="269" mass="30620" checksum="4A430429107E7C38" modified="2001-10-01" version="1">MIRRSQLYVPATSEKMIRKSVELKADSIIFDLEDAVPPEDKERARESLIKLVKELDWGKRELCVRVNSLQLLDSYKDISTIAREDKITCIVLPKAENDLSFLYKATGKSLIPLIETAKGLVKIEDIVRSEGVVAVSYGAADLSLSLGGDYNFYEKNVYVKTLIVSVARTYDVDAIDKVYFDLKNLDGFRKECEEAKKLGYVGKQVIHPSQIDISNEVFSPSKEEIEWAKKVIEAYEKGKREGRGAIRLDDKLVDYVHYKIAKRIIEFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YZ5</accession>
    <name>Q97YZ5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Monoamine oxidase regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90268">
        <property type="entry name" value="F90268"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YZ5"/>
    <dbReference type="FunCoup" id="Q97YZ5">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1154"/>
    <dbReference type="PaxDb" id="273057-SSO1154"/>
    <dbReference type="EnsemblBacteria" id="AAK41405">
        <property type="protein sequence ID" value="AAK41405"/>
        <property type="gene ID" value="SSO1154"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1154"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1148"/>
    <dbReference type="eggNOG" id="arCOG00774">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094876_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YZ5"/>
    <dbReference type="PhylomeDB" id="Q97YZ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052342">
        <property type="entry name" value="MCH/BMMD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43664:SF1">
        <property type="entry name" value="BETA-METHYLMALYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43664">
        <property type="entry name" value="MONOAMINE OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="70"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="163" mass="18611" checksum="0C9680542183F7E0" modified="2001-10-01" version="1">MRELLNFRNNYIAFLFSYNMFFDDFQVGQKWESKGRTITDADVVIFTGITGALNPLFLDEEYGKKTRFKGRIIPGLLTASIAIGLTYQLPVDPFGEGFVALTKLEMDAKKPVKIGDTLRAIVEVTDKKEREKDGRVYLTIRTLNQLGEEVMTLKMEILCNKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z02</accession>
    <name>Q97Z02_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1146</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90267">
        <property type="entry name" value="F90267"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z02"/>
    <dbReference type="STRING" id="273057.SSO1146"/>
    <dbReference type="PaxDb" id="273057-SSO1146"/>
    <dbReference type="EnsemblBacteria" id="AAK41397">
        <property type="protein sequence ID" value="AAK41397"/>
        <property type="gene ID" value="SSO1146"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1146"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1138"/>
    <dbReference type="eggNOG" id="arCOG02692">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1559558_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z02"/>
    <dbReference type="PhylomeDB" id="Q97Z02"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045:SF1">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="5">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="171" mass="19311" checksum="E3C46665F1A27B6E" modified="2001-10-01" version="1">MLIPNVYPLIISRLLQGLSLGGEWGGASTIVIEAYSEHKFRGFITSLLQLSVPIGIILSSLTILVIYNYSVYWPYSFLSITFLSLLSLLLVKDITTQKVTRSETQPLLEAKRHDWKNILKSIGIKVSESANFYIFTSYIFTNSSSISFLSLIVLISISFQLFLNPILWLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z10</accession>
    <name>Q97Z10_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90266">
        <property type="entry name" value="E90266"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989763.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z10"/>
    <dbReference type="STRING" id="273057.SSO1136"/>
    <dbReference type="PaxDb" id="273057-SSO1136"/>
    <dbReference type="EnsemblBacteria" id="AAK41388">
        <property type="protein sequence ID" value="AAK41388"/>
        <property type="gene ID" value="SSO1136"/>
    </dbReference>
    <dbReference type="GeneID" id="1454167"/>
    <dbReference type="KEGG" id="sso:SSO1136"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1128"/>
    <dbReference type="eggNOG" id="arCOG01933">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1313175_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z10"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="25902" checksum="C18546638F76FB24" modified="2001-10-01" version="1">MSFLFIDFNTHYGIRLYNYTEEPKLTEDEIEIKRILLNPIYKYSCDCCVDGFYQQYLWGRKDYTLKLGIYNPRCRVPPQVELIRQVDRGIIGVVLSPKHHNFSLLHSSLNFVYELIDKRGLILVLYDPSLDELRWLLDKYTFPVVLINSEHVVDNERVYYVVNHSSKIIDPRRSIYGSDAPYNSLNLIQSAKLFIKNHGYNEDVAYKNAIELLNHVNANF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z38</accession>
    <name>Q97Z38_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1101</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90262">
        <property type="entry name" value="G90262"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989734.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z38"/>
    <dbReference type="STRING" id="273057.SSO1101"/>
    <dbReference type="PaxDb" id="273057-SSO1101"/>
    <dbReference type="EnsemblBacteria" id="AAK41358">
        <property type="protein sequence ID" value="AAK41358"/>
        <property type="gene ID" value="SSO1101"/>
    </dbReference>
    <dbReference type="GeneID" id="1454137"/>
    <dbReference type="KEGG" id="sso:SSO1101"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1098"/>
    <dbReference type="eggNOG" id="arCOG02242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150444_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z38"/>
    <dbReference type="PhylomeDB" id="Q97Z38"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052362">
        <property type="entry name" value="HTH-GbsR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38465">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR MJ1563-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38465:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR MJ1563-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH marR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="127" mass="14596" checksum="95EDC3124F9709E9" modified="2001-10-01" version="1">MAEKVRFPDGREVDIHDFIAFMYGLSKSDVEVLHILLQNGKMTTDDLSQKLNVTKASISKALNNLLDKGLIQREKAPAEKEERKGRPNYIYWVEKERLYRKLEADLEKLAGTVKEALQKHTALEIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z39</accession>
    <name>Q97Z39_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90262">
        <property type="entry name" value="F90262"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989732.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z39"/>
    <dbReference type="PaxDb" id="273057-SSO1100"/>
    <dbReference type="EnsemblBacteria" id="AAK41357">
        <property type="protein sequence ID" value="AAK41357"/>
        <property type="gene ID" value="SSO1100"/>
    </dbReference>
    <dbReference type="GeneID" id="1454136"/>
    <dbReference type="KEGG" id="sso:SSO1100"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1096"/>
    <dbReference type="eggNOG" id="arCOG07849">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2313918_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z39"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="99" mass="11641" checksum="65F3A4595491AB11" modified="2001-10-01" version="1">MKVVKYNLKAKIERAGRDPITIERKFRKLVNARKFIDEVVGEDQVKCTPLSKSETLITKQCEGDQVKYFFEITIERIKKPKKEEKPEKEEKKQEQGTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZB7</accession>
    <name>Q97ZB7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acetylornithine deacetylase (ArgE-2)</fullName>
            <ecNumber evidence="9">3.5.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">argE-2</name>
        <name evidence="9" type="ordered locus">SSO1007</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.16" evidence="9"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90252">
        <property type="entry name" value="E90252"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989231.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZB7"/>
    <dbReference type="SMR" id="Q97ZB7"/>
    <dbReference type="FunCoup" id="Q97ZB7">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1007"/>
    <dbReference type="PaxDb" id="273057-SSO1007"/>
    <dbReference type="EnsemblBacteria" id="AAK41276">
        <property type="protein sequence ID" value="AAK41276"/>
        <property type="gene ID" value="SSO1007"/>
    </dbReference>
    <dbReference type="GeneID" id="1455243"/>
    <dbReference type="KEGG" id="sso:SSO1007"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1002"/>
    <dbReference type="eggNOG" id="arCOG01107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_2_3_2"/>
    <dbReference type="InParanoid" id="Q97ZB7"/>
    <dbReference type="PhylomeDB" id="Q97ZB7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008777">
        <property type="term" value="F:acetylornithine deacetylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006592">
        <property type="term" value="P:ornithine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010182">
        <property type="entry name" value="ArgE/DapE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01910">
        <property type="entry name" value="DapE-ArgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006401">
        <property type="entry name" value="PRK08651.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF32">
        <property type="entry name" value="ARGE_DAPE-RELATED DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0170">Cobalt</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="8">
        <location>
            <begin position="177"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="376" mass="42313" checksum="AEFF42735D6B0CBE" modified="2001-10-01" version="1">MLYPVKLLKDLVQIKTANPPGSEYESISFYLKDLFNDLGFQIQLIEIPEEYMDKYYIYSPSHKGNKRIIVIAKNSPNPILHFNFHYDVVPPGDGWLTNPFELKVVDNKAYGRGTSDMKGSIVSLYLALSRFNDLPIEIVFVPDEESGGIGTKYLTEEIRIAPSTAIFGEPSFPNVYIGHLGIVRGIIKIFGKQAHASNPKDGINAFLLASKLALKLQEKLGNEIVNLGGYTLNPTNNDGIVPGFFAFSYYRAIPPHDNRTPEFDKNVIDTTAREVGIEYNFEIKSFISGSVSNPDSSVAKAFKFCITSTLNVVPKELISNIRYDAVFYKNLDSINFGPGSLDQAHVPNEYVELDNIEKTIVIYECVMKRIYFNNYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZC2</accession>
    <name>Q97ZC2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Maltose transport inner membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1000</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90251">
        <property type="entry name" value="H90251"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZC2"/>
    <dbReference type="FunCoup" id="Q97ZC2">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1000"/>
    <dbReference type="TCDB" id="3.A.1.1.15">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1000"/>
    <dbReference type="EnsemblBacteria" id="AAK41271">
        <property type="protein sequence ID" value="AAK41271"/>
        <property type="gene ID" value="SSO1000"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1000"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.997"/>
    <dbReference type="eggNOG" id="arCOG00157">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_3_2"/>
    <dbReference type="InParanoid" id="Q97ZC2"/>
    <dbReference type="PhylomeDB" id="Q97ZC2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052730">
        <property type="entry name" value="Sugar_ABC_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43759:SF1">
        <property type="entry name" value="GLUCOSE IMPORT SYSTEM PERMEASE PROTEIN GLCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43759">
        <property type="entry name" value="TREHALOSE TRANSPORT SYSTEM PERMEASE PROTEIN SUGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="59"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="275" mass="29946" checksum="61D0B97EEFCD1A16" modified="2001-10-01" version="1">MNKVKLTFFLLVLPALAYVISFAFFPTIEAVYLSFQDPHGGFSLYNYKELSYFNLSSAIINTIVVTIGALAIQLALGFLVASVLSREFFGKRALSTITIIPMGIATVVAAVTFSFVFQTSGGYANTILHSLFGLNVNWYQSSISSLLVVMIADSWKNTPIVALILLAGMSSIPKELYYASAIDGAGPIRRFFYITLPNLRSFIGISLILRGVQEFNIFALPLILIGEHPPLLTTLIYDLYTTTFPEVGLALASATILLGFILVFSGIVIKLSGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZD5</accession>
    <name>Q97ZD5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oxidoreductase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0985</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DadA oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90250">
        <property type="entry name" value="B90250"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989259.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZD5"/>
    <dbReference type="STRING" id="273057.SSO0985"/>
    <dbReference type="PaxDb" id="273057-SSO0985"/>
    <dbReference type="DNASU" id="1455224"/>
    <dbReference type="EnsemblBacteria" id="AAK41257">
        <property type="protein sequence ID" value="AAK41257"/>
        <property type="gene ID" value="SSO0985"/>
    </dbReference>
    <dbReference type="GeneID" id="1455224"/>
    <dbReference type="KEGG" id="sso:SSO0985"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.982"/>
    <dbReference type="eggNOG" id="arCOG00758">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_753592_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZD5"/>
    <dbReference type="PhylomeDB" id="Q97ZD5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF286">
        <property type="entry name" value="D-AMINO ACID DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="5">
        <location>
            <begin position="3"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="365" mass="41129" checksum="4D090E31F578B307" modified="2001-10-01" version="1">MKVGIVGGGIVGLFTAYYLAKEGIKDIIIYEKGFLGAGSIHAAGLIEPYRFDKINTLSMIKKMLKYKMNGSTDIKEVDKLWLVELIKNLEKDPPGEAWDTMREMAKFSLSEYKRIAEEKNDFDYHEDGLLELYYNSKEFESGIEDEKKSPFNPKFEIEEIKGFAGGLFFPELSRISTEKFIDRISREIVSNIKVKNEEVIKVSDEGYIDGEKYDLVVVAAGIWSRILKIPLTAFKGYGYRVKGVSKLNRAAVIVDYGLAISPLSDHIKITGGFDADFSIDSKRAEDFLKKAEELVDIQYIIDLNMGFRPCSPDGFPVIGKRNNLVLVTGACRLGWSYGPAMGRYATDLALGKAKDLGYISRYYHE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2026-01-28" version="145" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZE7</accession>
    <name>SRP54_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Signal recognition particle 54 kDa protein</fullName>
            <shortName evidence="1">SRP54</shortName>
            <ecNumber evidence="1">3.6.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">srp54</name>
        <name type="ordered locus">SSO0971</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.4" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90248">
        <property type="entry name" value="F90248"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923154.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1QZW">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="4.10 A"/>
        <property type="chains" value="A/C/E/G=1-432"/>
    </dbReference>
    <dbReference type="PDB" id="1QZX">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="4.00 A"/>
        <property type="chains" value="A/B=1-432"/>
    </dbReference>
    <dbReference type="PDB" id="2IY3">
        <property type="method" value="EM"/>
        <property type="resolution" value="16.00 A"/>
        <property type="chains" value="A=297-432"/>
    </dbReference>
    <dbReference type="PDB" id="3KL4">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="A=2-432"/>
    </dbReference>
    <dbReference type="PDB" id="3ZN8">
        <property type="method" value="EM"/>
        <property type="resolution" value="12.00 A"/>
        <property type="chains" value="M=308-431"/>
    </dbReference>
    <dbReference type="PDB" id="5L3S">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A/C/E/G=1-292"/>
    </dbReference>
    <dbReference type="PDB" id="5L3V">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B=1-293"/>
    </dbReference>
    <dbReference type="PDBsum" id="1QZW"/>
    <dbReference type="PDBsum" id="1QZX"/>
    <dbReference type="PDBsum" id="2IY3"/>
    <dbReference type="PDBsum" id="3KL4"/>
    <dbReference type="PDBsum" id="3ZN8"/>
    <dbReference type="PDBsum" id="5L3S"/>
    <dbReference type="PDBsum" id="5L3V"/>
    <dbReference type="AlphaFoldDB" id="Q97ZE7"/>
    <dbReference type="EMDB" id="EMD-2316"/>
    <dbReference type="SMR" id="Q97ZE7"/>
    <dbReference type="FunCoup" id="Q97ZE7">
        <property type="interactions" value="279"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0971"/>
    <dbReference type="PaxDb" id="273057-SSO0971"/>
    <dbReference type="EnsemblBacteria" id="AAK41245">
        <property type="protein sequence ID" value="AAK41245"/>
        <property type="gene ID" value="SSO0971"/>
    </dbReference>
    <dbReference type="GeneID" id="1455212"/>
    <dbReference type="KEGG" id="sso:SSO0971"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.970"/>
    <dbReference type="eggNOG" id="arCOG01228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_6_0_2"/>
    <dbReference type="InParanoid" id="Q97ZE7"/>
    <dbReference type="PhylomeDB" id="Q97ZE7"/>
    <dbReference type="EvolutionaryTrace" id="Q97ZE7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000022">
        <property type="entry name" value="Signal recognition particle 54 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
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    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
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        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
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    <dbReference type="SUPFAM" id="SSF47446">
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    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0733">Signal recognition particle</keyword>
    <feature type="chain" id="PRO_0000101187" description="Signal recognition particle 54 kDa protein">
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            <begin position="1"/>
            <end position="447"/>
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            <begin position="103"/>
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        <ligand>
            <name>GTP</name>
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            <name>GTP</name>
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        <location>
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        <location>
            <begin position="411"/>
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    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
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    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="3KL4"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="5L3S"/>
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    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="5L3V"/>
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    </evidence>
    <sequence length="447" mass="50047" checksum="2A21A42E9E4ED9E2" modified="2001-10-01" version="1">MLENIRDAVRKFLTGSTPYEKAVDEFIKDLQKSLISSDVNVKLVFSLTAKIKERLNKEKPPSVLERKEWFISIVYDELSKLFGGDKEPNVNPTKLPFIIMLVGVQGSGKTTTAGKLAYFYKKRGYKVGLVAADVYRPAAYDQLLQLGNQIGVQVYGEPNNQNPIEIAKKGVDIFVKNKMDIIIVDTAGRHGYGEETKLLEEMKEMYDVLKPDDVILVIDASIGQKAYDLASRFHQASPIGSVIITKMDGTAKGGGALSAVVATGATIKFIGTGEKIDELETFNAKRFVSRILGMGDIESILEKVKGLEEYDKIQKKMEDVMEGKGKLTLRDVYAQIIALRKMGPLSKVLQHIPGLGIMLPTPSEDQLKIGEEKIRRWLAALNSMTYKELENPNIIDKSRMRRIAEGSGLEVEEVRELLEWYNNMNRLLKMVKRRRGNIDKLFGGKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZI7</accession>
    <name>Q97ZI7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">aminomethyltransferase</fullName>
            <ecNumber evidence="2">2.1.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glycine cleavage system T protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO0919</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[protein] + (6S)-5,6,7,8-tetrahydrofolate = N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16945"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10492"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="2.1.2.10"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.10" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="B90243"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923152.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZI7"/>
    <dbReference type="FunCoup" id="Q97ZI7">
        <property type="interactions" value="233"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0919"/>
    <dbReference type="PaxDb" id="273057-SSO0919"/>
    <dbReference type="EnsemblBacteria" id="AAK41201">
        <property type="protein sequence ID" value="AAK41201"/>
        <property type="gene ID" value="SSO0919"/>
    </dbReference>
    <dbReference type="GeneID" id="1455168"/>
    <dbReference type="KEGG" id="sso:SSO0919"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.920"/>
    <dbReference type="eggNOG" id="arCOG00756">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_10_2_2"/>
    <dbReference type="InParanoid" id="Q97ZI7"/>
    <dbReference type="PhylomeDB" id="Q97ZI7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:glycine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GcvT"/>
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        <property type="entry name" value="GcvT/YgfZ/DmdA"/>
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        <property type="entry name" value="GcvT/YgfZ_C"/>
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        <property type="entry name" value="GcvT_C"/>
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        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00528">
        <property type="entry name" value="gcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001567">
        <property type="entry name" value="PRK00389.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AMINOMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757:SF2">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GCV_T"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Aminomethyltransferase beta-barrel domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GCVT N-terminal" evidence="8">
        <location>
            <begin position="6"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Aminomethyltransferase C-terminal" evidence="9">
        <location>
            <begin position="268"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006487-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK41201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="346" mass="39724" checksum="1FEBA082081F0FC3" modified="2001-10-01" version="1">MFVSPFHDVEEKLGANFGEFAGWEMPMSFTSYAEEHMCVRNSVAFFNLSHMGRLRVKGNPREFEMLVAKEVNKANSGIMIGPTAFLNDKAGFKDDVMVYKVSENEWLIVTNAINREKIVNWIKSNSNLDVEDLTFKYGMIAIQGRKLEEILGKNELKQLEFKLNTRFMGYDVFLISRSGWTGENGLEVWVTLDVGRQLMMDLVKIGIKPAGLIARDSLRQEMGFVLYGEDIDETITPVEARYWVFSLEKDFIGKDSLVERIRSGIDKVRIGFKMRKGERTIPRHQSKIYSIGNEVGYVTSSTFSPYLNRVIGMGYVNPKYFYLGYSLTGEIRGKQYDIKIDEFPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZM8</accession>
    <name>Q97ZM8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90236">
        <property type="entry name" value="H90236"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992282.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZM8"/>
    <dbReference type="PaxDb" id="273057-SSO0860"/>
    <dbReference type="EnsemblBacteria" id="AAK41151">
        <property type="protein sequence ID" value="AAK41151"/>
        <property type="gene ID" value="SSO0860"/>
    </dbReference>
    <dbReference type="GeneID" id="1455118"/>
    <dbReference type="KEGG" id="sso:SSO0860"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.872"/>
    <dbReference type="eggNOG" id="arCOG00569">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143950_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZM8"/>
    <dbReference type="PhylomeDB" id="Q97ZM8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="123" mass="14036" checksum="7EE31D32C7236D33" modified="2001-10-01" version="1">MLNQPDFLRHIASKILSPLTIDSKRLDEARRILGEAEVKYNFSSYGGNPKKLIDFLLSPDFTELSLILGPDMTKKLLEAIKDNYTDEDIKKVVDKILEEINGYTENTEETNVKVSVNKKYLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZV7</accession>
    <name>Q97ZV7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NurA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0467</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90191">
        <property type="entry name" value="H90191"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922985.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZV7"/>
    <dbReference type="STRING" id="273057.SSO0467"/>
    <dbReference type="PaxDb" id="273057-SSO0467"/>
    <dbReference type="EnsemblBacteria" id="AAK40791">
        <property type="protein sequence ID" value="AAK40791"/>
        <property type="gene ID" value="SSO0467"/>
    </dbReference>
    <dbReference type="GeneID" id="84059043"/>
    <dbReference type="KEGG" id="sso:SSO0467"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.463"/>
    <dbReference type="eggNOG" id="arCOG05927">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1140565_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZV7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018977">
        <property type="entry name" value="NurA_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09376">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00933">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NurA" evidence="1">
        <location>
            <begin position="30"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="252" mass="29703" checksum="D5E9DD29592B7E00" modified="2001-10-01" version="1">MNMSSNIDSGDFSTIVRLRSNKNYFLNRELTFAAIDGTFSDIILEGEKGGYIVIGIIKGRIFKDFKFTIEDISVDDELCICEAEKRMRELEYYNIKENEVDLIFFDRKLSFDKSLGISVPKNSIGIVKDFDIVKRENLNNVENPPWLVINEKHDETIYGYFKLFPSSWVFYIESQVFVENPEELLSLIYNLGREPIPEALGYNYPLFLADKLVKYYRNKLSKFINVTSLQSNIRYREFRSWIERLRNDGKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZW6</accession>
    <name>Q97ZW6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5-formyltetrahydrofolate cyclo-ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90190">
        <property type="entry name" value="G90190"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988701.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZW6"/>
    <dbReference type="FunCoup" id="Q97ZW6">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0456"/>
    <dbReference type="PaxDb" id="273057-SSO0456"/>
    <dbReference type="EnsemblBacteria" id="AAK40782">
        <property type="protein sequence ID" value="AAK40782"/>
        <property type="gene ID" value="SSO0456"/>
    </dbReference>
    <dbReference type="GeneID" id="1455592"/>
    <dbReference type="KEGG" id="sso:SSO0456"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.451"/>
    <dbReference type="eggNOG" id="arCOG00474">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031500_2_0_2"/>
    <dbReference type="InParanoid" id="Q97ZW6"/>
    <dbReference type="PhylomeDB" id="Q97ZW6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10420:FF:000001">
        <property type="entry name" value="Methenyltetrahydrofolate synthase domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017:SF0">
        <property type="entry name" value="METHENYLTETRAHYDROFOLATE SYNTHASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="27544" checksum="A91D1D4C79814454" modified="2001-10-01" version="1">MLSKQEIRELIWRKLEEGNIALFPRPVYGRIPNFKGAEEAALNLVKCKEFENAEIIKVNPDSPQYKVREIALRHKKKVLVPTPRLRGDFFLLDPSRISPSDIPRASRISGFEKYGIRISLEDIVRVDFMVAGSVAVDINGNRVGKGEGYSELEFAILRELGKVDENTPIATTVHDVQIVDEIPSEPFDVPVDIIATPSRLIRVNRKREKPKGIYLEYLSKQKIDNTPLLREYLKKRGYNSM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-03-02" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZX2</accession>
    <name>ALAXM_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Alanyl-tRNA editing protein AlaX-M</fullName>
            <shortName>AlaX-M</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Alanyl-tRNA deacylase AlaX-M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">alaXM</name>
        <name type="ordered locus">SSO0450</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Proc. Natl. Acad. Sci. U.S.A." volume="100" first="15422" last="15427">
            <title>Trans-editing of mischarged tRNAs.</title>
            <authorList>
                <person name="Ahel I."/>
                <person name="Korencic D."/>
                <person name="Ibba M."/>
                <person name="Soll D."/>
            </authorList>
            <dbReference type="PubMed" id="14663147"/>
            <dbReference type="DOI" id="10.1073/pnas.2136934100"/>
        </citation>
        <scope>FUNCTION AS TRNA(ALA) EDITING PROTEIN</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90190">
        <property type="entry name" value="A90190"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988710.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZX2"/>
    <dbReference type="SMR" id="Q97ZX2"/>
    <dbReference type="FunCoup" id="Q97ZX2">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0450"/>
    <dbReference type="PaxDb" id="273057-SSO0450"/>
    <dbReference type="EnsemblBacteria" id="AAK40776">
        <property type="protein sequence ID" value="AAK40776"/>
        <property type="gene ID" value="SSO0450"/>
    </dbReference>
    <dbReference type="GeneID" id="1455586"/>
    <dbReference type="GeneID" id="44129432"/>
    <dbReference type="KEGG" id="sso:SSO0450"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.444"/>
    <dbReference type="eggNOG" id="arCOG01254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_3_2_2"/>
    <dbReference type="InParanoid" id="Q97ZX2"/>
    <dbReference type="PhylomeDB" id="Q97ZX2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.130:FF:000010">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053424">
        <property type="entry name" value="Alanyl-tRNA_Edit-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051335">
        <property type="entry name" value="Alanyl-tRNA_Editing_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040865">
        <property type="entry name" value="a_tRNA_ed_AlaXM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462:SF1">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN AARSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000391649" description="Alanyl-tRNA editing protein AlaX-M">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="14663147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="236" mass="26710" checksum="2AFC9CF180BF368E" modified="2001-10-01" version="1">MTEELYLKDSYIKEFEGRVVRIEGNYVILDKTAFYPGGGGLDNDTGFLVNEKGERISVTEVKRGENGEILHKIDQNGSLNVNEKVIGTIDWDRRYRMMRLHTASHIVAALAYRKFGALITGGHISPEQAKDDFNVENKDTLIELINEANEIIKKDIELKIYFLPREEALMIPAIVKLAGRNPPQIPIWRIVEIPGIDIQADGGPHVKNTKEIGEIVLLKVENKGKGRKRVYYTVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZX8</accession>
    <name>Q97ZX8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Agmatinase (Agmatine ureohydrolase) (SpeB-1)</fullName>
            <ecNumber evidence="6">3.5.3.11</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">speB-1</name>
        <name evidence="6" type="ordered locus">SSO0445</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="4">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.11" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90189">
        <property type="entry name" value="B90189"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922974.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZX8"/>
    <dbReference type="FunCoup" id="Q97ZX8">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0445"/>
    <dbReference type="PaxDb" id="273057-SSO0445"/>
    <dbReference type="EnsemblBacteria" id="AAK40769">
        <property type="protein sequence ID" value="AAK40769"/>
        <property type="gene ID" value="SSO0445"/>
    </dbReference>
    <dbReference type="GeneID" id="1455579"/>
    <dbReference type="KEGG" id="sso:SSO0445"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.437"/>
    <dbReference type="eggNOG" id="arCOG01700">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_0_2_2"/>
    <dbReference type="InParanoid" id="Q97ZX8"/>
    <dbReference type="PhylomeDB" id="Q97ZX8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11593">
        <property type="entry name" value="Agmatinase-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005925">
        <property type="entry name" value="Agmatinase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01230">
        <property type="entry name" value="agmatinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="297" mass="32973" checksum="A5EEAC683FF4FA95" modified="2001-10-01" version="1">MSDGRLLYLNENSRKFAGFNKEKSPFVIIGIPMDITSSYRPGSRFAPSSIRESAQYIEFYSIRNDVDMGEIGFNDVGDIILHPSNVEENLSRISSVISYFQESGKITISIGGEHTITAGIIKGTKREGLCLVSFDAHLDLRDEYMGYRYDHACVMRRISEYGVKIIEVGTRAVSKEEIEYAKQKGILFFTPQQIRLLGVKETARRIITSTQECKSLYISVDMDGIDPAYAPGVATPEPDGLDPTNLLDIINLIADKRVIGFDIVEVSPSYDTSGITSVLASRIILETAATVYKARSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q980B2</accession>
    <name>Q980B2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2208 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90184">
        <property type="entry name" value="E90184"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988785.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980B2"/>
    <dbReference type="STRING" id="273057.SSO0403"/>
    <dbReference type="PaxDb" id="273057-SSO0403"/>
    <dbReference type="EnsemblBacteria" id="AAK40732">
        <property type="protein sequence ID" value="AAK40732"/>
        <property type="gene ID" value="SSO0403"/>
    </dbReference>
    <dbReference type="GeneID" id="1455542"/>
    <dbReference type="KEGG" id="sso:SSO0403"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.399"/>
    <dbReference type="eggNOG" id="arCOG04319">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1105225_0_0_2"/>
    <dbReference type="InParanoid" id="Q980B2"/>
    <dbReference type="PhylomeDB" id="Q980B2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009198">
        <property type="entry name" value="UCP014484_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09973">
        <property type="entry name" value="DUF2208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF014484">
        <property type="entry name" value="UCP014484"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="29725" checksum="E94897096B94F19A" modified="2001-10-01" version="1">MSSTGYNPFNWKFVLLSQVMMILFSLVLSFFPKYFIEFYILYILVYLGITSVIMMRSNPLLRERRSLGEIANARTLYEEKKASELVNQDEEYLKETTEVMKKNFSSMGIMFLYMIILILVYNYVIIRFVTNIDNTLYKFGFFVLYFELLYGVSFLMNRRVLKFQTNIPMAPTSYKITEKGIIATDRSGVFLPSKYLIDAQISQNRDKKYIEIRSSSKFPFQVRLYSQDIDKVSELIERVKKIELKKQSSAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q980B3</accession>
    <name>Q980B3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GTP Cyclohydrolase II (3,4-Dihydroxy-2-Butanone 4-Phosphate Synthase (DHBP synthase) (RibA))</fullName>
            <ecNumber evidence="7">3.5.4.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ribA</name>
        <name evidence="7" type="ordered locus">SSO0402</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.25" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90184">
        <property type="entry name" value="D90184"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988787.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980B3"/>
    <dbReference type="FunCoup" id="Q980B3">
        <property type="interactions" value="143"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0402"/>
    <dbReference type="PaxDb" id="273057-SSO0402"/>
    <dbReference type="EnsemblBacteria" id="AAK40731">
        <property type="protein sequence ID" value="AAK40731"/>
        <property type="gene ID" value="SSO0402"/>
    </dbReference>
    <dbReference type="GeneID" id="1455541"/>
    <dbReference type="KEGG" id="sso:SSO0402"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.398"/>
    <dbReference type="eggNOG" id="arCOG01320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020273_3_2_2"/>
    <dbReference type="InParanoid" id="Q980B3"/>
    <dbReference type="PhylomeDB" id="Q980B3"/>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004437">
        <property type="entry name" value="PRK05773.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327:SF46">
        <property type="entry name" value="3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327">
        <property type="entry name" value="GTP CYCLOHYDROLASE II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0686">Riboflavin biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="219" mass="25157" checksum="D68675E0C15AE6DA" modified="2001-10-01" version="1">MDFLSLRKDLESGIPILIYDFDGREEETDMVFYAGAISWKSIYTLRKEAGGLICYATAINEAKTLGLNFIAEELKQHETYSKLVKKPSYGDYPAFSVWVNHVNTKTGISDYDRYITISELHKIILKTKTNPEEARREFYENFMTPGHVPILIARDIRERRGHTELSITLLQKLGLERSAVIAEILDEKSSMNKDKAKMIAKNFGLHFIEGKDIFKEVII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2026-01-28" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q980V0</accession>
    <name>RS21_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein aS21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 4" type="primary">rps21a</name>
        <name evidence="5" type="ordered locus">SSO5343</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="8 9 10" key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.90 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <scope>DISULFIDE BONDS</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9 10 11 12 13 14 15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8 9 10 12 13 14 15">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ribosomal protein aS21 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90158">
        <property type="entry name" value="C90158"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990401.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="C=1-65"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="C=1-65"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="C=1-65"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="C=1-65"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="C=1-65"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="C=1-65"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="C=1-65"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="C=1-65"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q980V0"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q980V0"/>
    <dbReference type="STRING" id="273057.SSO5343"/>
    <dbReference type="PaxDb" id="273057-SSO5343"/>
    <dbReference type="EnsemblBacteria" id="AAK40522">
        <property type="protein sequence ID" value="AAK40522"/>
        <property type="gene ID" value="SSO5343"/>
    </dbReference>
    <dbReference type="GeneID" id="1453349"/>
    <dbReference type="KEGG" id="sso:SSO5343"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.172"/>
    <dbReference type="eggNOG" id="arCOG01989">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196471_1_0_2"/>
    <dbReference type="InParanoid" id="Q980V0"/>
    <dbReference type="PhylomeDB" id="Q980V0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00872">
        <property type="entry name" value="Ribosomal_aS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044720">
        <property type="entry name" value="HVO_2753-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011668">
        <property type="entry name" value="HVO_2753-like_ZBP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011481">
        <property type="entry name" value="PRK14890.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40733:SF1">
        <property type="entry name" value="SMALL ZINC FINGER PROTEIN HVO-2753-LIKE ZINC-BINDING POCKET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40733">
        <property type="entry name" value="ZINC-RIBBON RNA-BINDING PROTEIN INVOLVED IN TRANSLATION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07754">
        <property type="entry name" value="HVO_2753_ZBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000463549" description="Small ribosomal subunit protein aS21">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 9 10">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9 10">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9 10">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9 10">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9 10">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9 10">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9 10">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9 10">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="10">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="11">
        <source>
            <dbReference type="PDB" id="9FRL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="12">
        <source>
            <dbReference type="PDB" id="9FS6"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="13">
        <source>
            <dbReference type="PDB" id="9FS8"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="14">
        <source>
            <dbReference type="PDB" id="9FSF"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="15">
        <source>
            <dbReference type="PDB" id="9FY0"/>
        </source>
    </evidence>
    <sequence length="65" mass="7341" checksum="7EFE07AD7AE73739" modified="2001-10-01" version="1">MSVKLSIREEVEPPICSSCGKIIHPREKGVEFYCPNCGEVLIRRDHMCRKQGAEYICPNCGFKGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Y5</accession>
    <name>Q980Y5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90153">
        <property type="entry name" value="H90153"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922895.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Y5"/>
    <dbReference type="PaxDb" id="273057-SSO0134"/>
    <dbReference type="EnsemblBacteria" id="AAK40487">
        <property type="protein sequence ID" value="AAK40487"/>
        <property type="gene ID" value="SSO0134"/>
    </dbReference>
    <dbReference type="GeneID" id="1453314"/>
    <dbReference type="KEGG" id="sso:SSO0134"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.129"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154343_0_0_2"/>
    <dbReference type="InParanoid" id="Q980Y5"/>
    <dbReference type="PhylomeDB" id="Q980Y5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="147" mass="16658" checksum="69D4B8E09AC66C37" modified="2001-10-01" version="1">MGFNDVFLLMGYTTSKLKNFTAFRKYKGSWGRKHGKSYFGFKVCNLVERRTNFVRGFKVGLANLSDLAFSFDNVKLMADRAWISRKDVLVKGVGSARLPVEGSGVKIREGKASSTTLRGVVMEVFFLNLYRDLEILSTRIRTKVLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Z5</accession>
    <name>Q980Z5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sun (Fmu) protein (Sun-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">sun-2</name>
        <name evidence="6" type="ordered locus">SSO0122</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90152">
        <property type="entry name" value="F90152"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988945.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Z5"/>
    <dbReference type="STRING" id="273057.SSO0122"/>
    <dbReference type="PaxDb" id="273057-SSO0122"/>
    <dbReference type="EnsemblBacteria" id="AAK40477">
        <property type="protein sequence ID" value="AAK40477"/>
        <property type="gene ID" value="SSO0122"/>
    </dbReference>
    <dbReference type="GeneID" id="1453304"/>
    <dbReference type="KEGG" id="sso:SSO0122"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.117"/>
    <dbReference type="eggNOG" id="arCOG00975">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_7_6_2"/>
    <dbReference type="InParanoid" id="Q980Z5"/>
    <dbReference type="PhylomeDB" id="Q980Z5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000735">
        <property type="entry name" value="Fmu (Sun) domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF70">
        <property type="entry name" value="TRNA_RRNA CYTOSINE-C5-METHYLASE, NOL1_NOP2_SUN FAMILY, FUSED TO N-TERMINAL NUSB REGULATOR DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="5">
        <location>
            <begin position="96"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="368" mass="43016" checksum="A8F4DB8C6ECA41A7" modified="2001-10-01" version="1">MKDIVKFLSTVLYYVEQKNYPLAIAFKRAYLHNKPRKIDSNLLYEYSKRLILSYYALPQSKRSAKVRYWLKNRENIEIKFPKWMEDKLFTLLDVNALKRKLTERWIWARINLLKTDIDKVYRMLESQNIEFEPDKDFYYMIKINRSSVRLSSLSIVKDFKLILHDKASSIVVEALKPEISEKLVDLSSAPGIKASLYMMLTENRAETFLADVDLKRLSKEYNLLKRCGVDLRKVHIIYQDSTKNSIIKSDKILLDAPCSSSGMINNDPSILLKLRNGEKIEKFTKLQKSLLNEALKIKSNKLVYAVCSLFPEEGEKVIEDYYEIAEKPFSNYQSGYSLFKSGKRSNRTFPHIDSTEGFFISTLNLTKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-25" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q981C9</accession>
    <name>QUEC_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>7-cyano-7-deazaguanine synthase</fullName>
            <ecNumber evidence="2">6.3.4.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>7-cyano-7-carbaguanine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Archaeosine biosynthesis protein QueC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>PreQ(0) synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">queC</name>
        <name type="ordered locus">SSO0016</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>7-carboxy-7-carbaguanine + NH4(+) + 2 ATP = 7-cyano-7-carbaguanine + 2 AMP + 2 diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:27982"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.20"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:27983"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>7-carboxy-7-carbaguanine + ATP + H(+) = 7-carboxyadenylyl-7-carbaguanine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:85175"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:234255"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:85176"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>7-carboxyadenylyl-7-carbaguanine + NH4(+) = 7-amido-7-carbaguanine + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:85179"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:190446"/>
            <dbReference type="ChEBI" id="CHEBI:234255"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:85180"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>7-amido-7-carbaguanine + ATP = 7-iminomethyladenylyl-7-carbaguanine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:85183"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:190446"/>
            <dbReference type="ChEBI" id="CHEBI:234257"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:85184"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>7-iminomethyladenylyl-7-carbaguanine = 7-cyano-7-carbaguanine + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:85187"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:234257"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:85188"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the QueC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.20" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90140">
        <property type="entry name" value="H90140"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989631.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981C9"/>
    <dbReference type="SMR" id="Q981C9"/>
    <dbReference type="STRING" id="273057.SSO0016"/>
    <dbReference type="PaxDb" id="273057-SSO0016"/>
    <dbReference type="DNASU" id="1455274"/>
    <dbReference type="EnsemblBacteria" id="AAK40383">
        <property type="protein sequence ID" value="AAK40383"/>
        <property type="gene ID" value="SSO0016"/>
    </dbReference>
    <dbReference type="GeneID" id="1455274"/>
    <dbReference type="GeneID" id="44128978"/>
    <dbReference type="KEGG" id="sso:SSO0016"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.17"/>
    <dbReference type="eggNOG" id="arCOG00039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_550565_0_0_2"/>
    <dbReference type="InParanoid" id="Q981C9"/>
    <dbReference type="BRENDA" id="2.6.1.97">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00352">
        <property type="entry name" value="Gn_AT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01995">
        <property type="entry name" value="QueC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01633">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00364">
        <property type="entry name" value="7-cyano-7-deazaguanine synthase QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914">
        <property type="entry name" value="7-CYANO-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914:SF1">
        <property type="entry name" value="7-CYANO-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000246990" description="7-cyano-7-deazaguanine synthase">
        <location>
            <begin position="2"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2">
        <location>
            <begin position="2"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="1">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="248"/>
            <end position="258"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01633"/>
        </source>
    </evidence>
    <sequence length="464" mass="52383" checksum="1B1EA2A9FA494ED4" modified="2001-10-01" version="1">MCSVSGVLILNPKNFEKVELKLASILKKAEDRGRDSFGIVVIQKDGTVKVRKSIGKPSEKEELLYGILDEDSRVVIANNRAEPTTEYVRQKTEDDIQPFIGDRYIVTHNGIIANDLELEKKYELKRKTKIDSAILPLLLDKTWDGNLEALKGILEQIKGSFALVIGDKKNPDRIFLAQNFKPLYMAYDHSLESLFFTSLDEYFDAKPFDPVNITKLEPYSVVMVTSNKLITTLPIMEKRKKYRVLVVASGGLDSTVAATKLLREGHEVTLIHFNYHHKAEEKEREAVRKIAEYLQIPLLEINTDLFKIIGHATLIKGGGEIVKDRKGEEGAEFAHEWVPARNLIFFSVSLAIAEAYGYDAIASGINLEEAGAYPDNEMEFIRMLNKLSPYATGPNKRIEILMPVGNLVKHEIVKLGYEIGAPLHLTWSCYEGGQKHCGKCGPCYMRKMAFRINGLKDPVEYDEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX95</accession>
    <name>RL24_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L24</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl24</name>
        <name evidence="1" type="synonym">rpl24Ab</name>
        <name type="ordered locus">SSO0707</name>
        <name type="ORF">C10_024</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Located at the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90219">
        <property type="entry name" value="A90219"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991270.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX95"/>
    <dbReference type="SMR" id="Q9UX95"/>
    <dbReference type="FunCoup" id="Q9UX95">
        <property type="interactions" value="259"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0707"/>
    <dbReference type="PaxDb" id="273057-SSO0707"/>
    <dbReference type="EnsemblBacteria" id="AAK41008">
        <property type="protein sequence ID" value="AAK41008"/>
        <property type="gene ID" value="SSO0707"/>
    </dbReference>
    <dbReference type="GeneID" id="1454975"/>
    <dbReference type="KEGG" id="sso:SSO0707"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.707"/>
    <dbReference type="eggNOG" id="arCOG04094">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093240_2_1_2"/>
    <dbReference type="InParanoid" id="Q9UX95"/>
    <dbReference type="PhylomeDB" id="Q9UX95"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000009">
        <property type="entry name" value="60S ribosomal protein L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_A">
        <property type="entry name" value="Ribosomal_uL24_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_uL24_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L26 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000130782" description="Large ribosomal subunit protein uL24">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="119" mass="13536" checksum="3525864889E05C42" modified="2000-05-01" version="1">MVSLKPSKQRKLLYTLPLHQRKKLLVAKVSDDIASQYGIKRIRVRKGDTVRVMRGGNSGKEGKVVEVNTKTGRLAIEGITRKKVDGTPVYVWIHASKVMITKLDLSDPRRREKLEKSKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXD4</accession>
    <name>RS3A_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein eS1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">30S ribosomal protein S3Ae</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ribosomal protein S1e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps3ae</name>
        <name type="ordered locus">SSO0746</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="3">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Located near the mRNA exit channel, it binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S small ribosomal subunit (PubMed:39753558). In inititation complexes on leaderless mRNA, it interacts with uS11 and eS26 (PubMed:39753558).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90223">
        <property type="entry name" value="B90223"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991323.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A=1-208"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A=1-208"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="A=1-208"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="A=1-208"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="A=1-208"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="A=1-208"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A=1-208"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="A=1-208"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q9UXD4"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q9UXD4"/>
    <dbReference type="FunCoup" id="Q9UXD4">
        <property type="interactions" value="192"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0746"/>
    <dbReference type="PaxDb" id="273057-SSO0746"/>
    <dbReference type="EnsemblBacteria" id="AAK41041">
        <property type="protein sequence ID" value="AAK41041"/>
        <property type="gene ID" value="SSO0746"/>
    </dbReference>
    <dbReference type="GeneID" id="1455008"/>
    <dbReference type="KEGG" id="sso:SSO0746"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.741"/>
    <dbReference type="eggNOG" id="arCOG04186">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062507_1_0_2"/>
    <dbReference type="InParanoid" id="Q9UXD4"/>
    <dbReference type="PhylomeDB" id="Q9UXD4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00359">
        <property type="entry name" value="Ribosomal_eS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001593">
        <property type="entry name" value="Ribosomal_eS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030838">
        <property type="entry name" value="Ribosomal_eS1_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003142">
        <property type="entry name" value="PRK04057.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11830">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01015">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01397">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000153561" description="Small ribosomal subunit protein eS1">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="CAB57557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="208" mass="23542" checksum="3837ABE7E53676B6" modified="2000-05-01" version="1">MSAKGGAIKDKWKMKKWYSVITPKAFGEVSLGSTPAYDITQTIGRRVETTLYDLTGDFSQVYVHLYFKIIGNEGDRLITRFVGHELSRDYLRSLIRRKSSKINSIFDVTTKDGYVVRVKGLVLTTYKCHQSQKTAIRKIINETVSKKASELSFDDFTQEVVFGRLANEIFEAAKKIYPLRKAEIEKTKVLKVPENLGKQVESSSVSSG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>P60833</accession>
    <name>RNP1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease P protein component 1</fullName>
            <shortName evidence="1">RNase P component 1</shortName>
            <ecNumber evidence="1">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Rpp29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnp1</name>
        <name type="ordered locus">SSO0710</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="unpublished observations" date="2004-03">
            <authorList>
                <person name="Coudert E."/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991273.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P60833"/>
    <dbReference type="SMR" id="P60833"/>
    <dbReference type="GeneID" id="24780171"/>
    <dbReference type="InParanoid" id="P60833"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00754">
        <property type="entry name" value="RNase_P_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023538">
        <property type="entry name" value="RNP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000128439" description="Ribonuclease P protein component 1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00754"/>
        </source>
    </evidence>
    <sequence length="79" mass="9506" checksum="D86F2AA5010AD50F" modified="2004-04-13" version="1">MILDYINSRIKILWYTDPFLISREGVIVLETEKTFLIKLEEKNKFIRIFKAHGIFEITFKGKSFIIAGYKLVRKPWKRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="142" xmlns="https://uniprot.org/uniprot">
    <accession>P95979</accession>
    <name>DPOL3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>DNA polymerase 3</fullName>
            <ecNumber>2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>DNA polymerase B3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>DNA polymerase III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">dpo3</name>
        <name type="ordered locus">SSO0081</name>
        <name type="ORF">C04041</name>
        <name type="ORF">C05_029</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1997" name="J. Bacteriol." volume="179" first="2632" last="2640">
            <title>Gene duplications in evolution of archaeal family B DNA polymerases.</title>
            <authorList>
                <person name="Edgell D.R."/>
                <person name="Klenk H.-P."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="9098062"/>
        </citation>
        <scope>DISCUSSION OF SEQUENCE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7"/>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75407">
        <property type="entry name" value="S75407"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988892.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95979"/>
    <dbReference type="SMR" id="P95979"/>
    <dbReference type="FunCoup" id="P95979">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0081"/>
    <dbReference type="PaxDb" id="273057-SSO0081"/>
    <dbReference type="EnsemblBacteria" id="AAK40441">
        <property type="protein sequence ID" value="AAK40441"/>
        <property type="gene ID" value="SSO0081"/>
    </dbReference>
    <dbReference type="GeneID" id="1455332"/>
    <dbReference type="KEGG" id="sso:SSO0081"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.80"/>
    <dbReference type="eggNOG" id="arCOG00328">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000203_6_0_2"/>
    <dbReference type="InParanoid" id="P95979"/>
    <dbReference type="PhylomeDB" id="P95979"/>
    <dbReference type="BRENDA" id="2.7.7.7">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05781">
        <property type="entry name" value="DNA_polB_B3_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000273">
        <property type="entry name" value="DNA polymerase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000046486" description="DNA polymerase 3">
        <location>
            <begin position="1"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="764" mass="88379" checksum="1A4325C7D9A4D672" modified="1997-05-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>P95991</accession>
    <name>RL13_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl13</name>
        <name evidence="1" type="synonym">rpl13Ab</name>
        <name type="ordered locus">SSO0069</name>
        <name type="ORF">C05002</name>
        <name type="ORF">C05_041</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75419">
        <property type="entry name" value="S75419"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988879.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95991"/>
    <dbReference type="SMR" id="P95991"/>
    <dbReference type="FunCoup" id="P95991">
        <property type="interactions" value="181"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0069"/>
    <dbReference type="PaxDb" id="273057-SSO0069"/>
    <dbReference type="EnsemblBacteria" id="AAK40431">
        <property type="protein sequence ID" value="AAK40431"/>
        <property type="gene ID" value="SSO0069"/>
    </dbReference>
    <dbReference type="GeneID" id="1455322"/>
    <dbReference type="KEGG" id="sso:SSO0069"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.70"/>
    <dbReference type="eggNOG" id="arCOG04242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076922_1_0_2"/>
    <dbReference type="InParanoid" id="P95991"/>
    <dbReference type="PhylomeDB" id="P95991"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_uL13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_uL13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_uL13_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005004">
        <property type="entry name" value="PRK06394.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000133765" description="Large ribosomal subunit protein uL13">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="149" mass="16855" checksum="8663E73439FA943B" modified="1997-05-01" version="1">MSTQVQEQVVIINAEGQILGRMASNVVRLLKEGKKVIIVNGEKAVISGEKNRVIESYKLLLTVKTLFNPYRNGIRRPRSPINIVKRTIRGMLPKSSKGRRMLKNVKIYVGVPKEFEGRQFIKFPDSDVSRLKGKYVTVEVVSKELGWSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX41</accession>
    <name>Q7LX41_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74072">
        <property type="entry name" value="S74072"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX41"/>
    <dbReference type="STRING" id="273057.SSO2165"/>
    <dbReference type="PaxDb" id="273057-SSO2165"/>
    <dbReference type="EnsemblBacteria" id="AAK42341">
        <property type="protein sequence ID" value="AAK42341"/>
        <property type="gene ID" value="SSO2165"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2165"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2258"/>
    <dbReference type="eggNOG" id="arCOG07304">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2032961_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX41"/>
    <dbReference type="PhylomeDB" id="Q7LX41"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="140" mass="15772" checksum="3DE4A5D80E3A56B7" modified="2011-05-31" version="1">MSDSCIKDKLYYISYQVKHMATKVLIKNMDEQLYRMLKARAALLGISVSEAIQQAISLWLNVTEGIGDYNYIVLKTNPEAINAYNEGKYVLACDGEYIGAFDSEKEAVEKGKGHKKCMIGSKNYDKQEIGDWGWSSIALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX57</accession>
    <name>Q7LX57_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74057">
        <property type="entry name" value="S74057"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923788.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX57"/>
    <dbReference type="PaxDb" id="273057-SSO2145"/>
    <dbReference type="EnsemblBacteria" id="AAK42323">
        <property type="protein sequence ID" value="AAK42323"/>
        <property type="gene ID" value="SSO2145"/>
    </dbReference>
    <dbReference type="GeneID" id="1453650"/>
    <dbReference type="KEGG" id="sso:SSO2145"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2240"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX57"/>
    <dbReference type="PhylomeDB" id="Q7LX57"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="80"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37384" checksum="D48266F6A52B9AC0" modified="2011-05-31" version="1">MQTIQVSKTELKSLAINLATNNINVISQDLDPEIVKAAPSLLTGNRGKYYLKVVRRGEKVISKGQRTFKFYPIYREVKGEINVVAVDETGLTVGEKEQEKAEGFLLYNWKRKGVKMRSLDLVYPLRLPLLVEVADLRSDSPSQFLLRSVREVSQYMEIDYVVADAGFLNLGVIKEMPVKTIVRGKSNLKGFKELSNVPLVEKRYEVKDKVYVAYRVLEFEGLYYYDVVYVKGKPRHFMFVTNFEGDPYELAELYRLRWQVEEGFKVRKARIRYVRKLSNKIFLFLYYTVLDSAWNLVNHLLFNFKSTCKKVLSFDSFVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX99</accession>
    <name>Q7LX99_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycogen debranching enzyme (TreX)</fullName>
            <ecNumber evidence="7">3.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">treX</name>
        <name evidence="7" type="ordered locus">SSO2094</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73088">
        <property type="entry name" value="S73088"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989784.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX99"/>
    <dbReference type="SMR" id="Q7LX99"/>
    <dbReference type="FunCoup" id="Q7LX99">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2094"/>
    <dbReference type="CAZy" id="CBM48">
        <property type="family name" value="Carbohydrate-Binding Module Family 48"/>
    </dbReference>
    <dbReference type="CAZy" id="GH13">
        <property type="family name" value="Glycoside Hydrolase Family 13"/>
    </dbReference>
    <dbReference type="MoonProt" id="Q7LX99"/>
    <dbReference type="PaxDb" id="273057-SSO2094"/>
    <dbReference type="EnsemblBacteria" id="AAK42273">
        <property type="protein sequence ID" value="AAK42273"/>
        <property type="gene ID" value="SSO2094"/>
    </dbReference>
    <dbReference type="GeneID" id="1453600"/>
    <dbReference type="KEGG" id="sso:SSO2094"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2172"/>
    <dbReference type="eggNOG" id="arCOG02949">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011725_1_1_2"/>
    <dbReference type="InParanoid" id="Q7LX99"/>
    <dbReference type="PhylomeDB" id="Q7LX99"/>
    <dbReference type="BRENDA" id="3.2.1.33">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902494">
        <property type="term" value="C:catalytic complex"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004135">
        <property type="term" value="F:amylo-alpha-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046562">
        <property type="term" value="F:beta-D-glucose oxidase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd11326">
        <property type="entry name" value="AmyAc_Glg_debranch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02856">
        <property type="entry name" value="E_set_GDE_Isoamylase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000468">
        <property type="entry name" value="Glycogen debranching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000054">
        <property type="entry name" value="Glycogen debranching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044505">
        <property type="entry name" value="GlgX_Isoamylase_N_E_set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011837">
        <property type="entry name" value="Glycogen_debranch_GlgX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02100">
        <property type="entry name" value="glgX_debranch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43002">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="6">
        <location>
            <begin position="178"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="718" mass="83091" checksum="43E7538DE8131788" modified="2011-05-31" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-06-18" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXH6</accession>
    <name>Q7LXH6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Orf in partial transposon ISC1913</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0797</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90229">
        <property type="entry name" value="H90229"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXH6"/>
    <dbReference type="STRING" id="273057.SSO0797"/>
    <dbReference type="PaxDb" id="273057-SSO0797"/>
    <dbReference type="EnsemblBacteria" id="AAK41095">
        <property type="protein sequence ID" value="AAK41095"/>
        <property type="gene ID" value="SSO0797"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0797"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.803"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_17_0_2"/>
    <dbReference type="InParanoid" id="Q7LXH6"/>
    <dbReference type="PhylomeDB" id="Q7LXH6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="3">
        <location>
            <begin position="274"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="371" mass="42531" checksum="182530ADCCED2340" modified="2011-05-31" version="1">MQVLSMIFTREILEEAINYGLTKNTTSFTRIKAGIYKTEREKHRDLPSHYIYTACEDASERLDSFNKLKKRGRSYTDRPSVRRVTVHLDDHLWKFSLNVISIATKRGRVCISPIFPKIFWRYYNDNWLIASGARFKLLKGNVVEFFIVFKKDVKSYDPRGFIPVDLNEDSVSVLINGKPILLETNTRMITLGYEYKRRSITNGRSTKDREVRRKLRERDKKLDVRRKFAKLIVKEAFGSRSAIVLEDLPRGVPEHMVKGVKDKQLRLRIYRSAFSSMKNAIVEKAREFGVPVILVDPSYTSSICPVHGSKIIYQPDGGSAPRVGVCGKGGERWHRDVVALYNLRKRAGDVSPVPLGSKESHDPPVVQVVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXJ2</accession>
    <name>Q7LXJ2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">polynucleotide 5'-hydroxyl-kinase</fullName>
            <ecNumber evidence="2">2.7.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">SSO0779</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of both single-stranded RNA (ssRNA) and single-stranded DNA (ssDNA). Exhibits a strong preference for ssRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5'-end dephospho-2'-deoxyribonucleoside-DNA + ATP = a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15669"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:13184"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:136416"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.78"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5'-end dephospho-ribonucleoside-RNA + ATP = a 5'-end 5'-phospho-ribonucleoside-RNA + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54580"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:15179"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.78"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.7.1.78" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90227">
        <property type="entry name" value="E90227"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923071.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXJ2"/>
    <dbReference type="SMR" id="Q7LXJ2"/>
    <dbReference type="FunCoup" id="Q7LXJ2">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0779"/>
    <dbReference type="PaxDb" id="273057-SSO0779"/>
    <dbReference type="EnsemblBacteria" id="AAK41076">
        <property type="protein sequence ID" value="AAK41076"/>
        <property type="gene ID" value="SSO0779"/>
    </dbReference>
    <dbReference type="GeneID" id="7806741"/>
    <dbReference type="KEGG" id="sso:SSO0779"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.780"/>
    <dbReference type="eggNOG" id="arCOG04127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051301_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LXJ2"/>
    <dbReference type="PhylomeDB" id="Q7LXJ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051734">
        <property type="term" value="F:ATP-dependent polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051731">
        <property type="term" value="F:polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF3">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-HYDROXYL-KINASE NOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Clp1 P-loop" evidence="10">
        <location>
            <begin position="84"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK41076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="360" mass="41440" checksum="9D7BBC20BBF927F2" modified="2011-05-31" version="1">MKKEQIIQGPCTIKVLEGEARILGIEIQKNDLLTIPSDKTYTLVYDENTRLETDCKKLLDNLNLRWDEIVEEIINTVGVVLLLGNVDSGKTYLTNLFTNLATSYLKIIDADVGQSTLFLPTFIAELKPMRKTLNLEELGYDTLEFFGDITPSTNPRLHVQKILRLYETTPKERLTVIDTDGWTVGLNSMLHKFELIYTIDPDYIIVFDQKIKDSLPENYRNKAILLKSVNLHKSRSERKANRIAKYERYFKEAKNIKTLSENLLGKQISNILYCAWGEYIQLSDEEPCTDYYIPLNLLKGALLGIIENKKVIGAALLDDLKENEITILSRVNKFTGLVLGYISLNDKFEERRIRFRKCKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXL1</accession>
    <name>Q7LXL1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0741</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90222">
        <property type="entry name" value="F90222"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991316.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXL1"/>
    <dbReference type="SMR" id="Q7LXL1"/>
    <dbReference type="STRING" id="273057.SSO0741"/>
    <dbReference type="PaxDb" id="273057-SSO0741"/>
    <dbReference type="EnsemblBacteria" id="AAK41037">
        <property type="protein sequence ID" value="AAK41037"/>
        <property type="gene ID" value="SSO0741"/>
    </dbReference>
    <dbReference type="GeneID" id="1455004"/>
    <dbReference type="KEGG" id="sso:SSO0741"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.737"/>
    <dbReference type="eggNOG" id="arCOG01042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131306_1_2_2"/>
    <dbReference type="InParanoid" id="Q7LXL1"/>
    <dbReference type="PhylomeDB" id="Q7LXL1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="PAB1135-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011144">
        <property type="entry name" value="PRK14555.2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38816:SF1">
        <property type="entry name" value="EXOSOME SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38816">
        <property type="entry name" value="EXOSOME SUBUNIT, DUF54 FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17367" checksum="248E35E72E128C21" modified="2011-05-31" version="1">MKINQAIISVFIHETEDYNKIVNTIESFFSPLISNSKKNVTTAQGHYGNKIIILEYRFDRKSGEQFFKIILEKIETSELMLILTTIDSHIDGSKLYLRFDKQYLIAEHRLVLKEGDDVIKCIISFNTSLENIKEEIKKLVNSRIMHSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-02-05" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXM6</accession>
    <name>Q7LXM6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO0686</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC-4 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90216">
        <property type="entry name" value="C90216"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXM6"/>
    <dbReference type="STRING" id="273057.SSO0686"/>
    <dbReference type="PaxDb" id="273057-SSO0686"/>
    <dbReference type="EnsemblBacteria" id="AAK40986">
        <property type="protein sequence ID" value="AAK40986"/>
        <property type="gene ID" value="SSO0686"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0686"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.685"/>
    <dbReference type="eggNOG" id="arCOG02312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_2"/>
    <dbReference type="InParanoid" id="Q7LXM6"/>
    <dbReference type="PhylomeDB" id="Q7LXM6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050250">
        <property type="entry name" value="Macrolide_Exporter_MacB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30572:SF4">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE YTRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30572">
        <property type="entry name" value="MEMBRANE COMPONENT OF TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="301"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="MacB-like periplasmic core" evidence="9">
        <location>
            <begin position="22"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="ABC3 transporter permease C-terminal" evidence="8">
        <location>
            <begin position="261"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK40986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="418" mass="42953" checksum="386C13A48C3EC9AD" modified="2011-05-31" version="1">MNSLDILWLAYKGLVSRKAIAILAIISVMIGVASVTVLVAFTQGVSQSVLSVVESLGPSTILVLPRGGASLTQATVATIASLPGIKAVYPVVSGFGEMNVEGQPIGVSIIGIDNLSALLGQVLLESGSVYPPVTSPETVIGSEVANPAPGIFFSPGDIITVQVSRGNSVALEVVGVLSPSGANPLSNSETSIFLPLNEAMAILNRTSYSELIINAQSVNDVNNVVNLIEEIYGNQLSVISVQQLINTVSTITSGFSFLLISVASISLFVGAVGIMAIMLSRVYQRIREIGIMKTVGLTTRDILLVFLAESGIIGLIGGIVGILVGLVGTSFIDLLSAITSQSASGTSTTVSSSGFRGGGGFGRFGTAASSSAFTFKPVISIEAILIALVVAVAVSLIAGIYPAWKAARLTAIDAIRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXN0</accession>
    <name>Q7LXN0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90215">
        <property type="entry name" value="G90215"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991228.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXN0"/>
    <dbReference type="PaxDb" id="273057-SSO0680"/>
    <dbReference type="EnsemblBacteria" id="AAK40982">
        <property type="protein sequence ID" value="AAK40982"/>
        <property type="gene ID" value="SSO0680"/>
    </dbReference>
    <dbReference type="GeneID" id="1454949"/>
    <dbReference type="KEGG" id="sso:SSO0680"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.681"/>
    <dbReference type="eggNOG" id="arCOG05943">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_968453_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXN0"/>
    <dbReference type="PhylomeDB" id="Q7LXN0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050834">
        <property type="entry name" value="Glycosyltransf_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43685">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43685:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="2">
        <location>
            <begin position="14"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="294" mass="34193" checksum="489A6EF5035E64BC" modified="2011-05-31" version="1">MVEIIIPVGPNDKLEWVKRSVNSALLQSVDKVIIYDNSERADIYNFFQELKDKLVYIKDKRMTKVNMARLRNKMLSLASDKYVIMLDSDVVIPKNYSNNIINKLERGVAYTWIHYAYSEEEIEKPLSISENNPNLGCAGLNLEVIKRIGYFDERYERDEDVWLYAKLKKTGYKVEPAEGRCLHLNKVHARLNLSSSVAEARRNLWRSKYDIMLMFDGLTDFTFLTGYSYYGSYYILAILSVIFHPLTFLYLPLIGYGVYYYKGPKKYLLNLIPGLSLAISFPYGLGYNLIQKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXQ7</accession>
    <name>Q7LXQ7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cupin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0648</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90212">
        <property type="entry name" value="B90212"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991185.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXQ7"/>
    <dbReference type="STRING" id="273057.SSO0648"/>
    <dbReference type="PaxDb" id="273057-SSO0648"/>
    <dbReference type="EnsemblBacteria" id="AAK40953">
        <property type="protein sequence ID" value="AAK40953"/>
        <property type="gene ID" value="SSO0648"/>
    </dbReference>
    <dbReference type="GeneID" id="1454920"/>
    <dbReference type="KEGG" id="sso:SSO0648"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.651"/>
    <dbReference type="eggNOG" id="arCOG03000">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507456_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXQ7"/>
    <dbReference type="PhylomeDB" id="Q7LXQ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047183">
        <property type="entry name" value="GDO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41517">
        <property type="entry name" value="1,2-DIOXYGENASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41517:SF1">
        <property type="entry name" value="CUPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-1" evidence="3">
        <location>
            <begin position="59"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="178" mass="20123" checksum="80485D9224DFC5AA" modified="2011-05-31" version="1">MEVLKRVDKQDVLKGEVEELVKQIEQDDLKVVTFMEYTNPPEKVKPKLIKFEKVLPLLERIAESGKIQNGVAKIMFFSPSTCRNRGLTPTMMAGFQLIKPGIATKPHSHNMASIYLVFRGRGYSVIGKEKIEWKAGDVFVVPANEIHYHVNTGDDDCILFDVTDSGLLESLGILEFRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXR6</accession>
    <name>Q7LXR6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0625</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90209">
        <property type="entry name" value="H90209"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991160.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXR6"/>
    <dbReference type="FunCoup" id="Q7LXR6">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0625"/>
    <dbReference type="PaxDb" id="273057-SSO0625"/>
    <dbReference type="EnsemblBacteria" id="AAK40935">
        <property type="protein sequence ID" value="AAK40935"/>
        <property type="gene ID" value="SSO0625"/>
    </dbReference>
    <dbReference type="GeneID" id="1454902"/>
    <dbReference type="KEGG" id="sso:SSO0625"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.631"/>
    <dbReference type="eggNOG" id="arCOG00352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011784_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXR6"/>
    <dbReference type="PhylomeDB" id="Q7LXR6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="2">
        <location>
            <begin position="157"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="332" mass="38194" checksum="71DC920207CCA61B" modified="2011-05-31" version="1">MLNPFEKLQIPPKANQTIDTMLKRLPKIGGTTVRDREIRRLKEYYERIKKYYDFVEQFPKIDELHPFYLEATRIMTDINKLRICLSVTKKTSVISMKILKKYINQIRKSPENESNRIMRQAFGRVSSVLRKSSECIDRVIDVAKELKKIQGIDPTLPTIVVAGPPNVGKSTLVSKISTAKPEIASYPFTTKEVHVGHVILDDFRIQVIDTPGILDRPEAERNRIERKATNAIRNLNGIIVFMFDSSISSVLSVEGQIELFREVKLLKKIVIPVINKIDEKYEEYYKKIVDFLLSEGNEWHEISAEKGIGIDKLKEELFNLVKSSGTNEVSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-03-27" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXS1</accession>
    <name>Q7LXS1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0617</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99209">
        <property type="entry name" value="A99209"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXS1"/>
    <dbReference type="STRING" id="273057.SSO0617"/>
    <dbReference type="PaxDb" id="273057-SSO0617"/>
    <dbReference type="EnsemblBacteria" id="AAK40928">
        <property type="protein sequence ID" value="AAK40928"/>
        <property type="gene ID" value="SSO0617"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0617"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.625"/>
    <dbReference type="eggNOG" id="arCOG03882">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881168_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXS1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="157" mass="18139" checksum="C5A56FF64DD1ABCF" modified="2011-05-31" version="1">MYEYFVIYDEILRIYMNFLQNLMLNYASRTINSDVEFMNIVLSDGSYIILEGDEKRVSIPFPKGIATTHTHPGICLFSHKDLETADHLFSIGYAVVSVMNTKCISSLYRRGVYTLDDKLALKNLVGKIKKAKNLEEVINIYRNLAFPNYLKFITYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXS4</accession>
    <name>Q7LXS4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0608</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90207">
        <property type="entry name" value="H90207"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991133.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXS4"/>
    <dbReference type="STRING" id="273057.SSO0608"/>
    <dbReference type="PaxDb" id="273057-SSO0608"/>
    <dbReference type="EnsemblBacteria" id="AAK40919">
        <property type="protein sequence ID" value="AAK40919"/>
        <property type="gene ID" value="SSO0608"/>
    </dbReference>
    <dbReference type="GeneID" id="1454886"/>
    <dbReference type="KEGG" id="sso:SSO0608"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.616"/>
    <dbReference type="eggNOG" id="arCOG05994">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2243667_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXS4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="103" mass="12124" checksum="BA6057D359854393" modified="2011-05-31" version="1">MSKFRIDKIPKNEQDIEEIQREIEESHPHEHEHEHSVEELIGEIYLNVQSLQSKVEELNKSNNNCKKEISKIYFILGKLLIALTTNDNDEKIKNLKEVLSLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXW2</accession>
    <name>Q7LXW2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl-CoA c-acetyltransferase (Acetoacetyl-CoA thiolase) (AcaB-1)</fullName>
            <ecNumber evidence="4">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">acaB-1</name>
        <name evidence="4" type="ordered locus">SSO0534</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.9" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90199">
        <property type="entry name" value="F90199"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991041.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXW2"/>
    <dbReference type="FunCoup" id="Q7LXW2">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0534"/>
    <dbReference type="PaxDb" id="273057-SSO0534"/>
    <dbReference type="EnsemblBacteria" id="AAK40853">
        <property type="protein sequence ID" value="AAK40853"/>
        <property type="gene ID" value="SSO0534"/>
    </dbReference>
    <dbReference type="GeneID" id="1454820"/>
    <dbReference type="KEGG" id="sso:SSO0534"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.532"/>
    <dbReference type="eggNOG" id="arCOG01278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_2"/>
    <dbReference type="InParanoid" id="Q7LXW2"/>
    <dbReference type="PhylomeDB" id="Q7LXW2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF6">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="2">
        <location>
            <begin position="38"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="3">
        <location>
            <begin position="265"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="359" mass="39648" checksum="46A03E68AE17E190" modified="2011-05-31" version="1">MVEVYLNSSSMIPIDRYYEYDIYDLVIEAYKNFEGEYDVNSIDLIVVANAYSQKLNNASLIAPKIAEKIGKPNVPSIKVDNADASGGAALLTAYSLIKSNMVNSVLIVGVEKQSDYPSKYLYDAISTNLDDYMFYNGISSHSVAALLMRQYMKRYGVDRNYFANWAIKMHKNATENPFAYLRFQVDLNTVINSQIISDPVRLFDVGARADGAAILLLSNNKTESAVKINKILSAQARYDFSPSLPSVRMLRDKLELKFSERTGIDIHDSYSIMAALMLEEAGIFESGKSLHNLDSVNVNLGGGLKARGHVGGASAIYQLAEAHMQLIGSFKGKKASVDNYIVISSDDLGMVSYGINLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXZ6</accession>
    <name>Q7LXZ6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0084</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75404">
        <property type="entry name" value="S75404"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988897.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXZ6"/>
    <dbReference type="STRING" id="273057.SSO0084"/>
    <dbReference type="PaxDb" id="273057-SSO0084"/>
    <dbReference type="EnsemblBacteria" id="AAK40444">
        <property type="protein sequence ID" value="AAK40444"/>
        <property type="gene ID" value="SSO0084"/>
    </dbReference>
    <dbReference type="GeneID" id="1453272"/>
    <dbReference type="KEGG" id="sso:SSO0084"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.83"/>
    <dbReference type="eggNOG" id="arCOG00932">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053467_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXZ6"/>
    <dbReference type="PhylomeDB" id="Q7LXZ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040087">
        <property type="entry name" value="MJ0021-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288">
        <property type="entry name" value="BIOTIN SYNTHASE-RELATED PROTEIN, RADICAL SAM SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288:SF1">
        <property type="entry name" value="GLYCYL-RADICAL ENZYME ACTIVATING ENZYME MJ0021-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01108">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="33"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="350" mass="39888" checksum="8492057FE68C2B9E" modified="2011-05-31" version="1">MTIIGNPEIGLYTGKLPKGCELCRLGGKLVLFITGECDDNCYYCPVSDDRFGKNKIFANETEVNNSMDVIYEAYRMKALGAGITGGDPILEIGRVIEIIEELKNEFGYDFHIHLYTTGRYVNDDIMRELVNAGLDEMRFHPVKELYLNAVKVALKYDIDIGLELPAIPNEKDKIVRLIKWAEENNVKFVNLNELELNSRNYLNLNSKGFHAKHGITGVEGSFETALDVLKKFEDNKKITVHYCSSIYKDLVETRTRFFRIIKYSSKPYEEYTNEATVVRAIVKTKGEIPILENFGESTGSDEYSISPSIVGDLLNDYTNLINEIYIVEEHPDSRRLRVGEKLIYSKSKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LYL5</accession>
    <name>Q7LYL5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription termination factor FttA</fullName>
            <ecNumber evidence="12">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">fttA</name>
        <name evidence="15" type="ordered locus">SSO0761</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Terminates transcription on the whole genome. Termination is linked to FttA-mediated RNA cleavage and does not require NTP hydrolysis. Cleaves endonucleolytically at the RNA exit channel of RNA polymerase (RNAP); the 5'-3' exonuclease activity of this protein degrades the nascent RNA released from RNAP.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 2 Zn(2+) ions, which are required for nuclease activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Interacts with RNA polymerase (RNAP), interacts with the Spt4-Spt5 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. FttA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="12"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99225">
        <property type="entry name" value="A99225"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LYL5"/>
    <dbReference type="FunCoup" id="Q7LYL5">
        <property type="interactions" value="180"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0761"/>
    <dbReference type="PaxDb" id="273057-SSO0761"/>
    <dbReference type="EnsemblBacteria" id="AAK41056">
        <property type="protein sequence ID" value="AAK41056"/>
        <property type="gene ID" value="SSO0761"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0761"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.758"/>
    <dbReference type="eggNOG" id="arCOG00543">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009673_5_1_2"/>
    <dbReference type="InParanoid" id="Q7LYL5"/>
    <dbReference type="PhylomeDB" id="Q7LYL5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004532">
        <property type="term" value="F:RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02410">
        <property type="entry name" value="KH-II_CPSF_arch_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16295">
        <property type="entry name" value="TTHA0252-CPSF-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00870">
        <property type="entry name" value="FttA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019975">
        <property type="entry name" value="aCPSF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033769">
        <property type="entry name" value="TffA_KH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03675">
        <property type="entry name" value="arCOG00543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF51">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17214">
        <property type="entry name" value="KH_TffA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 12" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="13">
        <location>
            <begin position="195"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="14">
        <location>
            <begin position="423"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="KHb" evidence="12">
        <location>
            <begin position="74"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="603"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAK41056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="639" mass="72059" checksum="4CCCE5354EDE67F5" modified="2011-05-31" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U02</accession>
    <name>Q97U02_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Polysaccharide biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90508">
        <property type="entry name" value="C90508"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U02"/>
    <dbReference type="STRING" id="273057.SSO3227"/>
    <dbReference type="PaxDb" id="273057-SSO3227"/>
    <dbReference type="EnsemblBacteria" id="AAK43322">
        <property type="protein sequence ID" value="AAK43322"/>
        <property type="gene ID" value="SSO3227"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3227"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3329"/>
    <dbReference type="eggNOG" id="arCOG07183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237969_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U02"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="25442" checksum="E3944A54FAEA1EC3" modified="2001-10-01" version="1">MAKLAEDKNPSIIVDAIKLYSIVGSLFLILIFLDGYGVLYKLRPEYIAAIFSLYILTFSNFIRGLYSIFYQSITMADPTLSVESKDEFKGYLFKLTTSNLLFSLLGLGISTLLIYFFSSYSPYLLAAFMSIGIITNSFSMLFTSYNVCKRIYNFRFPLREFLIPLGLSALSLPLSTVYRPVSFLPMGIYAVVAILVFTLYNYAFNPYGRRLVIAIIREFKQRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U14</accession>
    <name>Q97U14_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90506">
        <property type="entry name" value="E90506"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U14"/>
    <dbReference type="PaxDb" id="273057-SSO3212"/>
    <dbReference type="EnsemblBacteria" id="AAK43308">
        <property type="protein sequence ID" value="AAK43308"/>
        <property type="gene ID" value="SSO3212"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3212"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3314"/>
    <dbReference type="eggNOG" id="arCOG03869">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159101_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U14"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="144" mass="15885" checksum="70AF46A12FB9CA10" modified="2001-10-01" version="1">MGKILFILLRKNVLMAEKEVGIARLILRWITAMSSALWAGVHMVLTHAVLPNPTATMVYDTFFGFTSALAIIAAVLAIQGIKYSYPLITAFYAIDLTLLSETRLGPALLVGKKLPFNYYVDISLALDVILIVLSLILIFVEKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U39</accession>
    <name>Q97U39_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO3184</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90503">
        <property type="entry name" value="D90503"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U39"/>
    <dbReference type="STRING" id="273057.SSO3184"/>
    <dbReference type="PaxDb" id="273057-SSO3184"/>
    <dbReference type="EnsemblBacteria" id="AAK43283">
        <property type="protein sequence ID" value="AAK43283"/>
        <property type="gene ID" value="SSO3184"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3184"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3288"/>
    <dbReference type="eggNOG" id="arCOG03652">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_586107_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U39"/>
    <dbReference type="PhylomeDB" id="Q97U39"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="4"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="407" mass="43906" checksum="E0B6FFBB8C43FE0E" modified="2001-10-01" version="1">MSMYTKKLASAGGYYNLNYGAWRSKKLAFLEALTELVAYTLLNVVNVIAVYTILSALSDILGVVIPNYVIYFVIGFSIAYPTLISFTHVKKLLGYVVTISATAEAALLISLFFLSLSKGIHFDYFVPKFKNFGDLATAYVLTTVSISGAGAATYLGEETKKPHENISKGMWLALIIGGVSMFLGTYAMIVLWPGTISGLANSNQPLFVEMIQYGIIALYISLTLSINSLLASNIGTTIGAARILFNLARENAAPKIFTKVNRSGEPLVATAVIGISTAIITVLSVSVLGITGAFTEVAAVEGVLWLLGRILDGFGAPIFYWRINSLGIGNVVIPLIATIINSWGDAQSILQMDIVQLLMITVFASIVVSWYVLKARKGFPGTLVVDENNNVVTIDEYLKKIKAVNAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U48</accession>
    <name>Q97U48_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH hxlR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO3176</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90502">
        <property type="entry name" value="C90502"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991717.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U48"/>
    <dbReference type="SMR" id="Q97U48"/>
    <dbReference type="STRING" id="273057.SSO3176"/>
    <dbReference type="PaxDb" id="273057-SSO3176"/>
    <dbReference type="EnsemblBacteria" id="AAK43274">
        <property type="protein sequence ID" value="AAK43274"/>
        <property type="gene ID" value="SSO3176"/>
    </dbReference>
    <dbReference type="GeneID" id="1453193"/>
    <dbReference type="KEGG" id="sso:SSO3176"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3280"/>
    <dbReference type="eggNOG" id="arCOG01057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111585_5_3_2"/>
    <dbReference type="InParanoid" id="Q97U48"/>
    <dbReference type="PhylomeDB" id="Q97U48"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33204">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, MARR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33204:SF18">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="4">
        <location>
            <begin position="13"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="122" mass="13977" checksum="280B0EEF8C7F32DD" modified="2001-10-01" version="1">MRVSKSKKPTEICPIVSAIAIIGSEPKLLTIRYLLDGPKRFNELQRLTSLSSKTLSKTLKELENHGIVERKIINSHPIVAVYELTDKGKDLKRVFDELRRWGEKFALTKIAVMNSIRVNQEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U49</accession>
    <name>Q97U49_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90502">
        <property type="entry name" value="B90502"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991718.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U49"/>
    <dbReference type="STRING" id="273057.SSO3175"/>
    <dbReference type="PaxDb" id="273057-SSO3175"/>
    <dbReference type="EnsemblBacteria" id="AAK43273">
        <property type="protein sequence ID" value="AAK43273"/>
        <property type="gene ID" value="SSO3175"/>
    </dbReference>
    <dbReference type="GeneID" id="1453192"/>
    <dbReference type="KEGG" id="sso:SSO3175"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3279"/>
    <dbReference type="eggNOG" id="arCOG06001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_544713_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U49"/>
    <dbReference type="PhylomeDB" id="Q97U49"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="467" mass="52499" checksum="21A13080F451DA44" modified="2001-10-01" version="1">MKNKFIMYIILFLLLISLSISTIGNINIQKEQVIIQQYSTYLIQNGESLNYTVISGVEDKIIYNNASQVVIKYNETTYLLKPLGLTYYNNSIVNVTMDGDKLKIYSGIPQLVKAILYTSDMQEVVWAGYLSKPALISTYAYVNGSGTLELQYLNGSSIYSINISIGSSQKISVFLISLNYTMFIPRSYGNISISTELPTPYKSFENISSFYISMSSFYNNTLLPSIIWKSNNITAVEFFGINGKIVGFVEVNNTTLLILRDLNAEYYTPRYVIITTINNRTILLGIGNNSVESTDNITFSYFISSNRQEGLLFSVENNPSVFLAFNNGSVYDIEISYPKNISVSNITIQNTVFLAEKIAISLNSSFSLIPITLKYNGTFLILKELPNGSILFVNETDYFMHDNTLYLIAFEPAKAYYIVYSNHPLLTITQTKQAPIPYGANNLVLISGIVIILLVIASIFTIRKRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U51</accession>
    <name>Q97U51_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Second ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO3172</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90501">
        <property type="entry name" value="H90501"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924155.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U51"/>
    <dbReference type="STRING" id="273057.SSO3172"/>
    <dbReference type="PaxDb" id="273057-SSO3172"/>
    <dbReference type="EnsemblBacteria" id="AAK43271">
        <property type="protein sequence ID" value="AAK43271"/>
        <property type="gene ID" value="SSO3172"/>
    </dbReference>
    <dbReference type="GeneID" id="1453190"/>
    <dbReference type="KEGG" id="sso:SSO3172"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3276"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="InParanoid" id="Q97U51"/>
    <dbReference type="PhylomeDB" id="Q97U51"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF22">
        <property type="entry name" value="TNPB-LIKE PROTEIN MJ1635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="188"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="309"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK43271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="422" mass="50147" checksum="88A450D18F47D75B" modified="2001-10-01" version="1">MLKNLRIRKFEPEEEYVHFTYSIKNSEREKSKELIKEYRTLLQKAIDYLWNLTKIQVRKKNGSYKITLPKKKEVYKPLREELEKINHLASHYVDKAINDAFSILKSWRKRAIKGRASIEKPTLKKAYVRIKTTLRKVVGESVRITVRPYEYITFSWSKSWFSRRVRELELGEPIIKEEKVYLPFRYKLPWVTPVNFLAIDSNLYTLDAYDGEKFVTISLKQLYSLKYSMEVKRAKVQSFASKHTKRGRELMRKYSHRERNRVLDFVHKFVNTLLDLYPMTFFAVEKLNKESMFKDANGSLSRKISRTVWRSIHRVLKYKAPLYGSFVKEVNPHLTSRSCPRCGFVSRKVGKTFECERCGFKLDRQLNASLNIYLKMCGFPHIRDIPRVWVGVIPLMGRRGMNVRDFGEAQGLRIDIKYHEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U61</accession>
    <name>Q97U61_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glycolate oxidase iron-sulfur subunit. (GlcF)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">glcF</name>
        <name evidence="8" type="ordered locus">SSO3161</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HdrC family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90500">
        <property type="entry name" value="F90500"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989970.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U61"/>
    <dbReference type="STRING" id="273057.SSO3161"/>
    <dbReference type="PaxDb" id="273057-SSO3161"/>
    <dbReference type="EnsemblBacteria" id="AAK43261">
        <property type="protein sequence ID" value="AAK43261"/>
        <property type="gene ID" value="SSO3161"/>
    </dbReference>
    <dbReference type="GeneID" id="1453180"/>
    <dbReference type="KEGG" id="sso:SSO3161"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3266"/>
    <dbReference type="eggNOG" id="arCOG00333">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023081_0_1_2"/>
    <dbReference type="InParanoid" id="Q97U61"/>
    <dbReference type="PhylomeDB" id="Q97U61"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051460">
        <property type="entry name" value="HdrC_iron-sulfur_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255:SF1">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="41"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK43261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="344" mass="38749" checksum="A76AE93F11BCC605" modified="2001-10-01" version="1">MISSLKLTEMCTHCGFCLETCPTYVVTRNEVHSPRGRIEAVRIGINSTGFETCMYCRLCETACPAGVKYAQIITPVRKSSLKKTVMEKMLENPKLLAKSLKLLSKANSYPEVQRFVKFVKPNEVSEPLETDGEGDIILFPGCIESVIFRKTVEKAYKYLGKKYKVKIINGCCGLAHISNGNYEGAKKLAIRLAEKVKGKKVITLQSNCAAFMKEYKEWFGIEINAYDFAEFLIKEGIELPKVNDTFTVHYPCHAYRQKLVSHIRNVAEKMGIKIREMEDPHFECGAGGDYWLSHPKLSDMVMNVKRGKIAKSEAKKVIVTNSVCALAIRSMGYDVYHIADLLDT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-18" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U94</accession>
    <name>AGAL_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Alpha-galactosidase</fullName>
            <shortName>Alpha-Gal</shortName>
            <ecNumber>3.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">galS</name>
        <name type="ordered locus">SSO3127</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="2392" last="2399">
            <title>Identification of a novel alpha-galactosidase from the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Brouns S.J.J."/>
                <person name="Smits N."/>
                <person name="Wu H."/>
                <person name="Snijders A.P.L."/>
                <person name="Wright P.C."/>
                <person name="de Vos W.M."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="16547025"/>
            <dbReference type="DOI" id="10.1128/JB.188.7.2392-2399.2006"/>
        </citation>
        <scope>MUTAGENESIS OF ASP-367 AND ASP-425</scope>
        <scope>CHARACTERIZATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Involved in the degradation of various alpha-linked galactosides such as raffinose, stachyose and melibiose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text>Completely inhibited by most divalent metal cations, however magnesium and molybdene have no effect.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM>0.08 mM for para-nitrophenol-alpha-D-galactopyranoside</KM>
            <KM>37.4 mM for para-nitrophenol-beta-L-arabinopyranoside</KM>
        </kinetics>
        <phDependence>
            <text>Optimum pH is 5.0.</text>
        </phDependence>
        <temperatureDependence>
            <text>Optimum temperature is 90 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="2">Trimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 36 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK43227" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.2.1.22"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43227.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90496">
        <property type="entry name" value="D90496"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U94"/>
    <dbReference type="SMR" id="Q97U94"/>
    <dbReference type="STRING" id="273057.SSO3127"/>
    <dbReference type="CAZy" id="GH36">
        <property type="family name" value="Glycoside Hydrolase Family 36"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3127"/>
    <dbReference type="EnsemblBacteria" id="AAK43227">
        <property type="protein sequence ID" value="AAK43227"/>
        <property type="gene ID" value="SSO3127"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3127"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3232"/>
    <dbReference type="eggNOG" id="arCOG06049">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_415982_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U94"/>
    <dbReference type="BRENDA" id="3.2.1.22">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q97U94"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008811">
        <property type="entry name" value="Glycosyl_hydrolases_36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31268">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31268:SF32">
        <property type="entry name" value="GALACTINOL--SUCROSE GALACTOSYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05691">
        <property type="entry name" value="Raffinose_syn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0326">Glycosidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000232659" description="Alpha-galactosidase">
        <location>
            <begin position="1"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="425"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Hydrolase activity is below detection limit." evidence="1">
        <original>D</original>
        <variation>G</variation>
        <location>
            <position position="367"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Slightly increase of hydrolasee activity." evidence="1">
        <original>D</original>
        <variation>G</variation>
        <location>
            <position position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="16547025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="648" mass="74567" checksum="4263C97145745121" modified="2006-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UA7</accession>
    <name>Q97UA7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO11979</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90494">
        <property type="entry name" value="G90494"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990934.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UA7"/>
    <dbReference type="STRING" id="273057.SSO11979"/>
    <dbReference type="PaxDb" id="273057-SSO11979"/>
    <dbReference type="EnsemblBacteria" id="AAK43214">
        <property type="protein sequence ID" value="AAK43214"/>
        <property type="gene ID" value="SSO11979"/>
    </dbReference>
    <dbReference type="GeneID" id="15298637"/>
    <dbReference type="KEGG" id="sso:SSO11979"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3218"/>
    <dbReference type="eggNOG" id="arCOG08495">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2447801_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UA7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="89" mass="10359" checksum="B4EE747F4217C92C" modified="2001-10-01" version="1">MSFKFEDIKNILQNPSIKGFKVSVRKAVNFSESNTFQSISKTTVKEGTNFEGMWIKCIKERLECDVVTEKGDLYIINFKDKIIIKLEYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UE0</accession>
    <name>Q97UE0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Partial ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO11917</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90490">
        <property type="entry name" value="F90490"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UE0"/>
    <dbReference type="STRING" id="273057.SSO11917"/>
    <dbReference type="PaxDb" id="273057-SSO11917"/>
    <dbReference type="EnsemblBacteria" id="AAK43181">
        <property type="protein sequence ID" value="AAK43181"/>
        <property type="gene ID" value="SSO11917"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11917"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3183"/>
    <dbReference type="eggNOG" id="arCOG06508">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191160_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UE0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="86" mass="10414" checksum="A9B9F54294DCA594" modified="2001-10-01" version="1">MGKFHQRIQYPTRGCRKPLLMRRQCDVLYRITLMNELNEALDKVINKYKEKGFELKVGRGKNDKFEDYHIMNFFREGKKTLQTDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UH0</accession>
    <name>Q97UH0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dehydrogenase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO3049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90486">
        <property type="entry name" value="F90486"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924112.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UH0"/>
    <dbReference type="FunCoup" id="Q97UH0">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3049"/>
    <dbReference type="PaxDb" id="273057-SSO3049"/>
    <dbReference type="EnsemblBacteria" id="AAK43149">
        <property type="protein sequence ID" value="AAK43149"/>
        <property type="gene ID" value="SSO3049"/>
    </dbReference>
    <dbReference type="GeneID" id="7808586"/>
    <dbReference type="KEGG" id="sso:SSO3049"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3147"/>
    <dbReference type="eggNOG" id="arCOG01622">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_1_0_2"/>
    <dbReference type="InParanoid" id="Q97UH0"/>
    <dbReference type="PhylomeDB" id="Q97UH0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052515">
        <property type="entry name" value="Gfo/Idh/MocA_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43249:SF1">
        <property type="entry name" value="D-GLUCOSIDE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43249">
        <property type="entry name" value="UDP-N-ACETYL-2-AMINO-2-DEOXY-D-GLUCURONATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="20"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase C-terminal" evidence="2">
        <location>
            <begin position="160"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="371" mass="41760" checksum="DAAE16466D53306B" modified="2001-10-01" version="1">MKTILFFILIFNSQYMSKRIGVAVVGLGSIGKTHVKALKDLEKETEFVKLVAVVDQIKAIAEKIGSEYGTPYYTTIDEVLRNSEVDVISIATPSYLHAPQAILAIEYGKHVIVEKPMATTLAGAREMVSRAERNEVKLGVIFQERYAPDIRRLKNDILKELGRIYLIESELKWYRDMKGYYKRDEIARSWRGMWNTEGGGVMTNQGIHTIDLMIWLNGEVEEVSGFVDNLTHDGIEVEDTAVAIMRYKNGALGTISQTVSMKPTTYQYRKIRVNGSNGFVEITDGSLSTVAIEGKIEESKSSVEYKKETIAQPGNLHKELLRDFLRALSEDNDFPINGKEGMKSLEVIKAVYLSSVNRQVVKLPLDVRIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UH7</accession>
    <name>Q97UH7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SMP-30/Gluconolactonase/LRE-like region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO3041</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMP-30/CGR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90485">
        <property type="entry name" value="G90485"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990697.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UH7"/>
    <dbReference type="FunCoup" id="Q97UH7">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3041"/>
    <dbReference type="PaxDb" id="273057-SSO3041"/>
    <dbReference type="EnsemblBacteria" id="AAK43142">
        <property type="protein sequence ID" value="AAK43142"/>
        <property type="gene ID" value="SSO3041"/>
    </dbReference>
    <dbReference type="GeneID" id="1453061"/>
    <dbReference type="KEGG" id="sso:SSO3041"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3139"/>
    <dbReference type="eggNOG" id="arCOG05370">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036110_3_1_2"/>
    <dbReference type="InParanoid" id="Q97UH7"/>
    <dbReference type="PhylomeDB" id="Q97UH7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004341">
        <property type="term" value="F:gluconolactonase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019853">
        <property type="term" value="P:L-ascorbic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000139">
        <property type="entry name" value="SMP-30/Gluconolaconase/LRE domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005511">
        <property type="entry name" value="SMP-30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907:SF47">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01790">
        <property type="entry name" value="SMP30FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="4">
        <location>
            <begin position="15"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="285" mass="32805" checksum="416D9E8FA6A446ED" modified="2001-10-01" version="1">MEIELKTLGNYKAILGESPVYDREGNRLFWVDIDGKKVIVNNLDTNEEKYYDTPDLVTSLCLIDYKRIIVTLRHGFHTLNLTTGEITPYLELENNGENRFNDGKCDIRDRYWAGTMNLHIENPKPTASLYKLEGQRLTKVLDKLYRSNGLGWDPDNKLFYLIDTPLRKVFQFDFDKDKGDIYNKRIVMDFKYEPGRPDGMTIDEEGYLWIAHSAGGKVSRWNPKNGEKVSEIKLPVLYVSSVTFGSPEMNQIFITTMSKGGEPLAGRLFTAKMNIKGLQSYKFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UK7</accession>
    <name>Q97UK7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycosyltransferase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO3002</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90481">
        <property type="entry name" value="B90481"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UK7"/>
    <dbReference type="FunCoup" id="Q97UK7">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3002"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3002"/>
    <dbReference type="EnsemblBacteria" id="AAK43105">
        <property type="protein sequence ID" value="AAK43105"/>
        <property type="gene ID" value="SSO3002"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3002"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3094"/>
    <dbReference type="eggNOG" id="arCOG01389">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_659913_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UK7"/>
    <dbReference type="PhylomeDB" id="Q97UK7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050501">
        <property type="term" value="F:hyaluronan synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085029">
        <property type="term" value="P:extracellular matrix assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030213">
        <property type="term" value="P:hyaluronan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06434">
        <property type="entry name" value="GT2_HAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22913">
        <property type="entry name" value="HYALURONAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22913:SF12">
        <property type="entry name" value="MANNURONAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13641">
        <property type="entry name" value="Glyco_tranf_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="438"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="470" mass="54329" checksum="128D333546833880" modified="2001-10-01" version="1">MKIFYINAFIVNTRTYMRLDFTLYLLISSLISIFTILYISFTLPLAFTYKPKSSQTNIKRSEITVLIPVYKEDVDVFKEVINSVKKQDLNFVVVGDGCDEPYKSITISKGGLFVKLDKNMGKRRAIREGFKYVKTPYVLLLDSDTILPDNSIDILSSKLAGDIVAVSPEISILPGRNKVTFHISEMMQRLREISYRALARFGSIVSLNGQCILAKTDVIKSLVESEEFNSVKLWRFSTILGDDRQITNYIYSKGYKATVTNDVVVKTKAPDTIRDLLKQMVRWYRSNNFFLIKEIGDGTIIKKGFFYMFTVLYWYALPLLTLLSYSLYTEIILSHAIKHWNFIVRAITTDPARFIENVIISRIDRLFNIDPTPIANKFHHFLPHHFNPIMLIYDNKLSAKMILFHYFYVFHTLVGEVSAIVSIVMIISILLYTRKIRGFALGLLAFPLMFLADIFALMTIWRQKKWSGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V60</accession>
    <name>Q97V60_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ferredoxin like protein (Zfx-like1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">zfx-like1</name>
        <name evidence="7" type="ordered locus">SSO11114</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90453">
        <property type="entry name" value="F90453"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990222.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V60"/>
    <dbReference type="STRING" id="273057.SSO11114"/>
    <dbReference type="PaxDb" id="273057-SSO11114"/>
    <dbReference type="EnsemblBacteria" id="AAK42885">
        <property type="protein sequence ID" value="AAK42885"/>
        <property type="gene ID" value="SSO11114"/>
    </dbReference>
    <dbReference type="GeneID" id="1452804"/>
    <dbReference type="KEGG" id="sso:SSO11114"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2857"/>
    <dbReference type="eggNOG" id="arCOG01985">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163428_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V60"/>
    <dbReference type="PhylomeDB" id="Q97V60"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012206">
        <property type="entry name" value="Fd_FixX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43082">
        <property type="entry name" value="FERREDOXIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43082:SF3">
        <property type="entry name" value="FERREDOXIN-LIKE PROTEIN YDIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036548">
        <property type="entry name" value="Fdx_FixX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="19"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="51"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="89" mass="9903" checksum="EAA67F172E83A0D2" modified="2001-10-01" version="1">MMDLLKRLSLNKYNVDKTTHIEVNTDICLTCKDKPCTKVCPAGTYEPSPDGRIVVHYERCLECGAALVACPYGAIKFHFPEGGISYKYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VR7</accession>
    <name>Q97VR7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90427">
        <property type="entry name" value="C90427"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VR7"/>
    <dbReference type="STRING" id="273057.SSO2545"/>
    <dbReference type="PaxDb" id="273057-SSO2545"/>
    <dbReference type="EnsemblBacteria" id="AAK42674">
        <property type="protein sequence ID" value="AAK42674"/>
        <property type="gene ID" value="SSO2545"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2545"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2626"/>
    <dbReference type="eggNOG" id="arCOG07324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103622_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VR7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="233" mass="26061" checksum="5AE2B8EC87EB4A18" modified="2001-10-01" version="1">MFIFLFQLALSLTLVAMSESSLISGTFVIYNGTSTYYLQNGSILFQKFSIIQVINKTFNNGTVLVGISIISWNTSMQSKFLYSVMNSTLPLPFYYVSPYLLGEENISVNEVELFYVNHTNSYYVYLGKGNIDGVIIKTYFYFKPDGLADKVINLQLGANGKIVSETTLALWITNFNNTSIGIPHFKGITTASASNEISVSLFPVYAKIMEYIIMAGAILVMIILLFRGTKRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VS1</accession>
    <name>Q97VS1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2541</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90426">
        <property type="entry name" value="G90426"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991643.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VS1"/>
    <dbReference type="PaxDb" id="273057-SSO2541"/>
    <dbReference type="EnsemblBacteria" id="AAK42670">
        <property type="protein sequence ID" value="AAK42670"/>
        <property type="gene ID" value="SSO2541"/>
    </dbReference>
    <dbReference type="GeneID" id="1453997"/>
    <dbReference type="KEGG" id="sso:SSO2541"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2619"/>
    <dbReference type="eggNOG" id="arCOG07323">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_985584_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VS1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="277" mass="31995" checksum="7EDE6EAF9700B332" modified="2001-10-01" version="1">MEIEIKTPFASVRINNDNKQSEIINGKDRIVIGRIYYYLTKTIFLIPRLYGIIAKDPLVDWKNEFERQFTHILTNELTLAKLLTLELYFRITSLKMSITGSIQNGKVEAKVELKTLPEIKQQEDKIRSLVRIDSFYFSDINKKRPHIIPAIRAGLIASFYKFLPIKFEGAPGIPKTLGIISDFINSMVLPQGYSEEVLGHKIYIKDDEVYCDDNILYNADPTVLSLFPIVYFIKNSSENDIIVIEEHEVHLEEFKEILKEILNKSRAKLVLVSNKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VU9</accession>
    <name>Q97VU9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acetyl-CoA c-acetyltransferase (Acetoacetyl-CoA thiolase) (AcaB-6)</fullName>
            <ecNumber evidence="3">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">acaB-6</name>
        <name evidence="3" type="ordered locus">SSO2508</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.9" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90423">
        <property type="entry name" value="B90423"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989900.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VU9"/>
    <dbReference type="STRING" id="273057.SSO2508"/>
    <dbReference type="PaxDb" id="273057-SSO2508"/>
    <dbReference type="DNASU" id="1453968"/>
    <dbReference type="EnsemblBacteria" id="AAK42641">
        <property type="protein sequence ID" value="AAK42641"/>
        <property type="gene ID" value="SSO2508"/>
    </dbReference>
    <dbReference type="GeneID" id="1453968"/>
    <dbReference type="KEGG" id="sso:SSO2508"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2587"/>
    <dbReference type="eggNOG" id="arCOG01281">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_2_1_2"/>
    <dbReference type="InParanoid" id="Q97VU9"/>
    <dbReference type="PhylomeDB" id="Q97VU9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF2">
        <property type="entry name" value="LIPID-TRANSFER PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase C-terminal" evidence="2">
        <location>
            <begin position="237"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="380" mass="42506" checksum="2DEA1C2037450986" modified="2001-10-01" version="1">MIVGFAGKLYKNYEKDGIELLKEVTDEALDIAGLNYADVDGIMANIGRGSFHGNRTYLNPPAQISEYFGIKPKIIEHVQYGGPSVLSMIYRAYKAIKAGDAETILCIQGGKISHLRDNHFQKTDIIDSPFDEYIKIYDQMSPISDYAMVAYRHSKLFGTTDEQRARIAVIQRYNASYNPKALFRNPITVEDVLKSRIVSYPLHLLEIVYPIDGFHVFVVSKRSSKSALRNIDILAYGEAHWPNPPAEWDDIIYTPTTESAKRASFDLNRVDAFQLYDSFTITVMLQMEDIGLVEKGKVGQFVESHDLSFKGDVPINTGGGSLNTGQPAYMSGGVILEEALLQLNDMANGHQVKGANVIFLNGIGWWSRRHSVTLVLGERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VW3</accession>
    <name>Q97VW3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2491</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90421">
        <property type="entry name" value="D90421"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923895.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VW3"/>
    <dbReference type="STRING" id="273057.SSO2491"/>
    <dbReference type="PaxDb" id="273057-SSO2491"/>
    <dbReference type="EnsemblBacteria" id="AAK42627">
        <property type="protein sequence ID" value="AAK42627"/>
        <property type="gene ID" value="SSO2491"/>
    </dbReference>
    <dbReference type="GeneID" id="15296765"/>
    <dbReference type="KEGG" id="sso:SSO2491"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2572"/>
    <dbReference type="eggNOG" id="arCOG05962">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1811467_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VW3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="148" mass="17905" checksum="1879CA9260CE5A97" modified="2001-10-01" version="1">MIEWKGFGKRWGKCEECWLAYERRIQHENSLNCYKLGIPIDALKIPLDQFLNIVKDVPGKYAIFGFPLNLLSKGVIIFYFDTKEEMENFIENIMNYIKSEISFREKKFYDIFVNTEWIGSMNWRRGCPEYDKKFGDWRGWRNHSNEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VW6</accession>
    <name>Q97VW6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Quinol oxidase-2, sulfocyanin (Blue copper protein), putative (SoxE-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">soxE-like</name>
        <name evidence="5" type="ordered locus">SSO2488</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99421">
        <property type="entry name" value="A99421"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VW6"/>
    <dbReference type="STRING" id="273057.SSO2488"/>
    <dbReference type="PaxDb" id="273057-SSO2488"/>
    <dbReference type="EnsemblBacteria" id="AAK42624">
        <property type="protein sequence ID" value="AAK42624"/>
        <property type="gene ID" value="SSO2488"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2488"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2569"/>
    <dbReference type="eggNOG" id="arCOG03700">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105306_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VW6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04230">
        <property type="entry name" value="Sulfocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010532">
        <property type="entry name" value="SoxE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049544">
        <property type="entry name" value="SoxE-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034246">
        <property type="entry name" value="Sulfocyanin_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06525">
        <property type="entry name" value="SoxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022145">
        <property type="entry name" value="Sulfocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfocyanin-like C-terminal" evidence="4">
        <location>
            <begin position="102"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="67"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="261" mass="27349" checksum="83FD6DAFD760AACD" modified="2001-10-01" version="1">MGKHLNTVPWDLVVMNTSIIIAVIVILIIVVGIVAYLTLVHHPATISSTTSSVETTSTSFTLTSTSTTISTSSTSTSSTTTTSSSTTSSTTITTTQSVTLPPGANMLPYNPNNKTVFIYLAVTATGPAFNYNGTSNGQMKIYVPAGWNVMIILTNEQSIPHNANIVLNDTQIPNNPNISADGKILLYVGDSPSNFANNGILSGQTAVGILDNISAGYYWIACGITGHAKNGMWADLIVSNSVSVPYFIISSSSSSTSTTHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VX6</accession>
    <name>Q97VX6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Metabolite permease, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2476</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90419">
        <property type="entry name" value="G90419"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923882.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VX6"/>
    <dbReference type="FunCoup" id="Q97VX6">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2476"/>
    <dbReference type="PaxDb" id="273057-SSO2476"/>
    <dbReference type="EnsemblBacteria" id="AAK42614">
        <property type="protein sequence ID" value="AAK42614"/>
        <property type="gene ID" value="SSO2476"/>
    </dbReference>
    <dbReference type="GeneID" id="1453941"/>
    <dbReference type="KEGG" id="sso:SSO2476"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2555"/>
    <dbReference type="eggNOG" id="arCOG01316">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018808_15_0_2"/>
    <dbReference type="InParanoid" id="Q97VX6"/>
    <dbReference type="PhylomeDB" id="Q97VX6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050277">
        <property type="entry name" value="Sodium:Solute_Symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086:SF8">
        <property type="entry name" value="MONOCARBOXYLIC ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086">
        <property type="entry name" value="SODIUM/PROLINE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="197"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="242"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="328"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="401"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="464"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="505" mass="54270" checksum="102EF180E88B6517" modified="2001-10-01" version="1">MLGLAGIIVFIALFIVFIFLGFYGAFWRKGNLDLLHEWGLAGRRLGTVLVWFLMGADLYTAYTFIAVPSSLYAKGAIYFFAVPYVSLTFAIAMLTMPKLWIVSRNRGYVTAADFVKDRFNSSILAALIALTGVVAELPYIALQIVGMKSVLQMMLLGIGNATLVSEISLIVSFIILAAFTYTSGLRGAALTGVFKDILIWITVISVIVIVPLQYGGFTHAFSVINSANLSARYTTLPSNLQIAYWSLFLGSALALYLYPHSINGSLSADDKNRLMYSTALLPIYGIGLAIIALFGILVYAVPNALSVVSKYGGALVVPALITSTMPDWAAGIMLLGIFVGGLVPAAIMAIAASNLLVRNVIKEVNPSITPKGETTLAKWISAVIKFLALAFVFLVPSTYAINLQLLGGILITQTLPAVFLGLYTKWFNPWALTAGWTTGIISGTYMFFTFGLPRATSLYPTLFGLMYAAVIALVLNLVVSVIGTLVANALGFKTKSNISAEDLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W18</accession>
    <name>Q97W18_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc finger ZPR1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2428</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ZPR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90414">
        <property type="entry name" value="E90414"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W18"/>
    <dbReference type="STRING" id="273057.SSO2428"/>
    <dbReference type="PaxDb" id="273057-SSO2428"/>
    <dbReference type="DNASU" id="1453899"/>
    <dbReference type="EnsemblBacteria" id="AAK42572">
        <property type="protein sequence ID" value="AAK42572"/>
        <property type="gene ID" value="SSO2428"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2428"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2506"/>
    <dbReference type="eggNOG" id="arCOG04265">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107446_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W18"/>
    <dbReference type="PhylomeDB" id="Q97W18"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1040">
        <property type="entry name" value="ZPR1, A/B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004457">
        <property type="entry name" value="Znf_ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040141">
        <property type="entry name" value="ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004470">
        <property type="entry name" value="ZPR1-like_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042451">
        <property type="entry name" value="ZPR1_A/B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056180">
        <property type="entry name" value="ZPR1_jr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00340">
        <property type="entry name" value="zpr1_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00310">
        <property type="entry name" value="ZPR1_znf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22794">
        <property type="entry name" value="jr-ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00709">
        <property type="entry name" value="Zpr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="5">
        <location>
            <begin position="15"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="174" mass="19715" checksum="7EF650EEF017D49E" modified="2001-10-01" version="1">MTVSEEPKVIFEETLICPVCKSKTLKAVDYLYNTPHTGKLVLSNWYCENCGYKFRDVKPYETREPKLVEMKIENEDDLSALVYRSAFAKIVIPELGIEIEPAGMSQGYISTIEGILEILLDQVGNFCDKECEDRIRSAMEGKIKFTLIIEDESGLSFIKSEKASTRPLILTNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W24</accession>
    <name>Q97W24_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2422</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable oxidoreductase that may play a role as regulator of mitochondrial function.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with COX5B; this interaction may contribute to localize PYROXD2 to the inner face of the inner mitochondrial membrane.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90413">
        <property type="entry name" value="G90413"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989407.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W24"/>
    <dbReference type="FunCoup" id="Q97W24">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2422"/>
    <dbReference type="PaxDb" id="273057-SSO2422"/>
    <dbReference type="EnsemblBacteria" id="AAK42566">
        <property type="protein sequence ID" value="AAK42566"/>
        <property type="gene ID" value="SSO2422"/>
    </dbReference>
    <dbReference type="GeneID" id="1453893"/>
    <dbReference type="KEGG" id="sso:SSO2422"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2500"/>
    <dbReference type="eggNOG" id="arCOG01521">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019327_0_1_2"/>
    <dbReference type="InParanoid" id="Q97W24"/>
    <dbReference type="PhylomeDB" id="Q97W24"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10668">
        <property type="entry name" value="PHYTOENE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10668:SF103">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="4">
        <location>
            <begin position="13"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00040298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="518" mass="57945" checksum="B538CF7B74ACD72B" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W27</accession>
    <name>Q97W27_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thioredoxin reductase (TrxB-3)</fullName>
            <ecNumber evidence="7">1.8.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">trxB-3</name>
        <name evidence="7" type="ordered locus">SSO2416</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.1.9" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90413">
        <property type="entry name" value="D90413"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989411.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W27"/>
    <dbReference type="SMR" id="Q97W27"/>
    <dbReference type="FunCoup" id="Q97W27">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2416"/>
    <dbReference type="PaxDb" id="273057-SSO2416"/>
    <dbReference type="EnsemblBacteria" id="AAK42563">
        <property type="protein sequence ID" value="AAK42563"/>
        <property type="gene ID" value="SSO2416"/>
    </dbReference>
    <dbReference type="GeneID" id="1453890"/>
    <dbReference type="KEGG" id="sso:SSO2416"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2498"/>
    <dbReference type="eggNOG" id="arCOG01296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_3_2"/>
    <dbReference type="InParanoid" id="Q97W27"/>
    <dbReference type="PhylomeDB" id="Q97W27"/>
    <dbReference type="BRENDA" id="1.8.1.9">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="1.8.1.B4">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="16"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="323" mass="35221" checksum="A363EF9D5FA913A5" modified="2001-10-01" version="1">MSLLPRTTSVKPGEKFDVIIVGLGPAAYGAALYSARYMLKTLVIGETPGGQLTEAGIVDDYLGLIEIQASDMIKVFNKHIEKYEVPVLLDIVEKIENRGDEFVVKTKRKGEFKADSVILGIGVKRRKLGVPGEQEFAGRGISYCSVCDAPLFKNRVVAVIGGGDSALEGAEILSSYSTKVYLIHRRDTFKAQPIYVETVKKKPNVEFVLNSVVKEIKGDKVVKQVVVENLKTGEIKELNVNGVFIEIGFDPPTDFAKSNGIETDTNGYIKVDEWMRTSVPGVFAAGDCTSAWLGFRQVITAVAQGAVAATSAYRYVTEKKGKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-10" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W59</accession>
    <name>IF2B_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 2 subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">aIF2-beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">eIF-2-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif2b</name>
        <name type="synonym">aif2b</name>
        <name type="ordered locus">SSO2381</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="13366" last="13366">
            <title>Cryo-EM study of start codon selection during archaeal translation initiation.</title>
            <authorList>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Monestier A."/>
                <person name="Larquet E."/>
                <person name="Cladiere L."/>
                <person name="Klaholz B.P."/>
                <person name="Schmitt E."/>
                <person name="Mechulam Y."/>
            </authorList>
            <dbReference type="PubMed" id="27819266"/>
            <dbReference type="DOI" id="10.1038/ncomms13366"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (5.34 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Assembles into a translation intitiation complex with leadered or leaderless mRNA, 30S ribosomes and tRNA(Met)i (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90409">
        <property type="entry name" value="B90409"/>
    </dbReference>
    <dbReference type="PDB" id="2NXU">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=2-139"/>
    </dbReference>
    <dbReference type="PDB" id="2QMU">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.20 A"/>
        <property type="chains" value="C=2-139"/>
    </dbReference>
    <dbReference type="PDB" id="2QN6">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.15 A"/>
        <property type="chains" value="C=2-19"/>
    </dbReference>
    <dbReference type="PDB" id="3CW2">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="K/L/M/N=1-139"/>
    </dbReference>
    <dbReference type="PDB" id="3V11">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="5.00 A"/>
        <property type="chains" value="C=2-139"/>
    </dbReference>
    <dbReference type="PDB" id="5JB3">
        <property type="method" value="EM"/>
        <property type="resolution" value="5.34 A"/>
        <property type="chains" value="8=1-139"/>
    </dbReference>
    <dbReference type="PDB" id="5JBH">
        <property type="method" value="EM"/>
        <property type="resolution" value="5.34 A"/>
        <property type="chains" value="8=1-139"/>
    </dbReference>
    <dbReference type="PDB" id="5K0Y">
        <property type="method" value="EM"/>
        <property type="resolution" value="5.80 A"/>
        <property type="chains" value="d=3-19"/>
    </dbReference>
    <dbReference type="PDB" id="6SW9">
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        <property type="resolution" value="4.20 A"/>
        <property type="chains" value="8=3-139"/>
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    <dbReference type="PDB" id="6SWC">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.30 A"/>
        <property type="chains" value="8=1-139"/>
    </dbReference>
    <dbReference type="PDBsum" id="2NXU"/>
    <dbReference type="PDBsum" id="2QMU"/>
    <dbReference type="PDBsum" id="2QN6"/>
    <dbReference type="PDBsum" id="3CW2"/>
    <dbReference type="PDBsum" id="3V11"/>
    <dbReference type="PDBsum" id="5JB3"/>
    <dbReference type="PDBsum" id="5JBH"/>
    <dbReference type="PDBsum" id="5K0Y"/>
    <dbReference type="PDBsum" id="6SW9"/>
    <dbReference type="PDBsum" id="6SWC"/>
    <dbReference type="AlphaFoldDB" id="Q97W59"/>
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    <dbReference type="EMDB" id="EMD-10320"/>
    <dbReference type="EMDB" id="EMD-10322"/>
    <dbReference type="EMDB" id="EMD-8148"/>
    <dbReference type="EMDB" id="EMD-8149"/>
    <dbReference type="SMR" id="Q97W59"/>
    <dbReference type="DIP" id="DIP-46185N"/>
    <dbReference type="FunCoup" id="Q97W59">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="IntAct" id="Q97W59">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2381"/>
    <dbReference type="PaxDb" id="273057-SSO2381"/>
    <dbReference type="EnsemblBacteria" id="AAK42529">
        <property type="protein sequence ID" value="AAK42529"/>
        <property type="gene ID" value="SSO2381"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2381"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2463"/>
    <dbReference type="eggNOG" id="arCOG01640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026663_3_1_2"/>
    <dbReference type="InParanoid" id="Q97W59"/>
    <dbReference type="PhylomeDB" id="Q97W59"/>
    <dbReference type="BRENDA" id="3.6.5.3">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97W59"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="DisProt" id="DP01285"/>
    <dbReference type="FunFam" id="3.30.30.170:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00232">
        <property type="entry name" value="eIF_2_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004458">
        <property type="entry name" value="TIF2_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003067">
        <property type="entry name" value="PRK03988.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
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    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000137434" description="Translation initiation factor 2 subunit beta">
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            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="4"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="21"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="39"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="42"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="47"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="50"/>
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        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="59"/>
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        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="62"/>
            <end position="65"/>
        </location>
    </feature>
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        <location>
            <begin position="73"/>
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        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="76"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="82"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="88"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="95"/>
            <end position="99"/>
        </location>
    </feature>
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        <location>
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            <end position="103"/>
        </location>
    </feature>
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            <end position="112"/>
        </location>
    </feature>
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        <location>
            <begin position="114"/>
            <end position="116"/>
        </location>
    </feature>
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        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="125"/>
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        </location>
    </feature>
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            <begin position="133"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_00232"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="3">
        <source>
            <dbReference type="PDB" id="5JB3"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="4">
        <source>
            <dbReference type="PDB" id="5JBH"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="2NXU"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="2QMU"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="2QN6"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="3CW2"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="6SWC"/>
        </source>
    </evidence>
    <sequence length="139" mass="15920" checksum="A5C60230FDAE921C" modified="2001-10-01" version="1">MSSEKEYVEMLDRLYSKLPEKGRKEGTQSLPNMIILNIGNTTIIRNFAEYCDRIRREDKICMKYLLKELAAPGNVDDKGELVIQGKFSSQVINTLMERFLKAYVECSTCKSLDTILKKEKKSWYIVCLACGAQTPVKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WC2</accession>
    <name>Q97WC2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AAK42466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="C90401"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="AAK42466">
        <property type="protein sequence ID" value="AAK42466"/>
        <property type="gene ID" value="SSO2310"/>
    </dbReference>
    <dbReference type="GeneID" id="1453793"/>
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    <dbReference type="PATRIC" id="fig|273057.12.peg.2399"/>
    <dbReference type="eggNOG" id="arCOG00498">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF14">
        <property type="entry name" value="METALLO-BETA-LACTAMASE SUPERFAMILY PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="Lactamase_B"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="272" mass="30480" checksum="C5927D46D197526B" modified="2001-10-01" version="1">MPTVKLTKNITIMTGSPNTLIYDNRVVIDLGGKNSSVDINAEVQLATHGHADHIAGLLKKDAKIRYLPKEDYWSLTLMGRRAMIYGCSSKDSDIFTFDYVKENIESIDIEVRTSEIEVIKLPGHTPGHSGYIVDNVLYAGDAFFGEKVLEGFSVPFYTDFWTALESLNKVKELAKSVNNIVISHGPVYTNKNKMVSILESNIIYAQNLIGKILDMLSNNELTVEEIVVKLKQDLTPSNVLLNSVVVRSILFGLENIEYNVSQKGLVFRRRVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WP8</accession>
    <name>Q97WP8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transport protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2074</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99375">
        <property type="entry name" value="A99375"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WP8"/>
    <dbReference type="STRING" id="273057.SSO2074"/>
    <dbReference type="PaxDb" id="273057-SSO2074"/>
    <dbReference type="EnsemblBacteria" id="AAK42256">
        <property type="protein sequence ID" value="AAK42256"/>
        <property type="gene ID" value="SSO2074"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2074"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2151"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035002_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WP8"/>
    <dbReference type="PhylomeDB" id="Q97WP8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050171">
        <property type="entry name" value="MFS_Transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517:SF3">
        <property type="entry name" value="INTEGRAL MEMBRANE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517">
        <property type="entry name" value="RESISTANCE PROTEIN MDTM, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="349"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="197"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="366" mass="40878" checksum="0EEBB97DE773CACC" modified="2001-10-01" version="1">MMNRQFILFTILVFFTGLYLGTLRIIIPVFEKQINISIMLSLLLPLVSFGFVKGAFNFIAGKLSDDLGRKRVLVIGWLVALISVPLFLSINIYTVIIISILLAINQALTWTTTVTSQIDISGKLRAGFATGINEMSGYLGVSFGSLFASYLFKLSSIFIGIICLIALISSFNVIETKTLIPNATLSKKENNHINYFSITKISIAGLLEKFVDSAFFILIPTFLLLQHYTLFLIGITVSSYTFTWSLSQPLFGYLADTYNKRRLILVIGFLLMFVGFIKYSELPILFSIIEGIGMGMIYPNLIAFVNDKINESVRGKALGYYRLYRDSGYGVAGLLLPLLYSFYGYEYTLLIVGILQVVALLLVVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WX9</accession>
    <name>Q97WX9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90364">
        <property type="entry name" value="E90364"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990957.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WX9"/>
    <dbReference type="STRING" id="273057.SSO1981"/>
    <dbReference type="PaxDb" id="273057-SSO1981"/>
    <dbReference type="EnsemblBacteria" id="AAK42172">
        <property type="protein sequence ID" value="AAK42172"/>
        <property type="gene ID" value="SSO1981"/>
    </dbReference>
    <dbReference type="GeneID" id="1453499"/>
    <dbReference type="KEGG" id="sso:SSO1981"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2057"/>
    <dbReference type="eggNOG" id="arCOG07852">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_479535_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WX9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="72"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="568" mass="63994" checksum="D32E40D02D097985" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WZ5</accession>
    <name>Q97WZ5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposase ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1966</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90362">
        <property type="entry name" value="E90362"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923701.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WZ5"/>
    <dbReference type="STRING" id="273057.SSO1966"/>
    <dbReference type="PaxDb" id="273057-SSO1966"/>
    <dbReference type="EnsemblBacteria" id="AAK42156">
        <property type="protein sequence ID" value="AAK42156"/>
        <property type="gene ID" value="SSO1966"/>
    </dbReference>
    <dbReference type="GeneID" id="1453483"/>
    <dbReference type="KEGG" id="sso:SSO1966"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2039"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066381_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WZ5"/>
    <dbReference type="PhylomeDB" id="Q97WZ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="3">
        <location>
            <begin position="194"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="316" mass="35906" checksum="0ED4680258F908EA" modified="2001-10-01" version="1">MEAPVAGIDVSKDKLIVYFQGKLYEFTNDKRGYEEIRKILPKGCKVGIESTGVYHVNLAKYLNNEYDVRIINPFILKKFKDFRGKKSDKNDAKKLAELVVSMCSGFTTSDARELTSQWDFVTRSIARVKNRLRRDLVLLGYRDSLSKKNLEEVLRGGDSIVLAEVRFLLEELERLEGRKREIEKELEDFVSKDSLIFTIPGIGKTLGCIILARVGDVKRFSDKKRFVAYCGLDPVVESSGKSVVSRGISKKGDAVLRRAFYLAALTAIKVNPVIKRFYEEHKGRLKGKKLITACARKLAVITWAVLYYNKPFDASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WZ7</accession>
    <name>Q97WZ7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4143 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90362">
        <property type="entry name" value="C90362"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WZ7"/>
    <dbReference type="STRING" id="273057.SSO1963"/>
    <dbReference type="PaxDb" id="273057-SSO1963"/>
    <dbReference type="DNASU" id="1453481"/>
    <dbReference type="EnsemblBacteria" id="AAK42154">
        <property type="protein sequence ID" value="AAK42154"/>
        <property type="gene ID" value="SSO1963"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1963"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2037"/>
    <dbReference type="eggNOG" id="arCOG03168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151713_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WZ7"/>
    <dbReference type="PhylomeDB" id="Q97WZ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025420">
        <property type="entry name" value="DUF4143"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295:SF19">
        <property type="entry name" value="ARCHAEAL ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13635">
        <property type="entry name" value="DUF4143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4143" evidence="1">
        <location>
            <begin position="9"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="141" mass="16727" checksum="7133E66A9B969753" modified="2001-10-01" version="1">MTTAFRPRYGEKPRERLTFNKKVYVVDQGIISRVALRAKDRGRVMENLVALKLLRDNQLKGLYYVKGKDFEIDFYDEVNSRLLQVTYASDKIEEREIRSLLKAEEMLRTKELIMITYDIEGEEEREGKKIKLVPLYKFLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WZ9</accession>
    <name>Q97WZ9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1961</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99362">
        <property type="entry name" value="A99362"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923699.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WZ9"/>
    <dbReference type="STRING" id="273057.SSO1961"/>
    <dbReference type="PaxDb" id="273057-SSO1961"/>
    <dbReference type="EnsemblBacteria" id="AAK42152">
        <property type="protein sequence ID" value="AAK42152"/>
        <property type="gene ID" value="SSO1961"/>
    </dbReference>
    <dbReference type="GeneID" id="1453479"/>
    <dbReference type="KEGG" id="sso:SSO1961"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2035"/>
    <dbReference type="eggNOG" id="arCOG06007">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1860786_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WZ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="138" mass="15223" checksum="C5E8CD01AF385B05" modified="2001-10-01" version="1">MVEHNSRILGYNIATSIGYSFILTIIMAIVSLIVKAFYPPSTFDISPIEAMIHSPAEGIVQILILVILVVFVFPTRTNLEKSSLIQVRKIALITASSYLVFSLLPYAFQVSYPQTYLGLTIAYNLLNGTFAGFISTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X00</accession>
    <name>Q97X00_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90361">
        <property type="entry name" value="H90361"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991750.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X00"/>
    <dbReference type="STRING" id="273057.SSO1960"/>
    <dbReference type="PaxDb" id="273057-SSO1960"/>
    <dbReference type="EnsemblBacteria" id="AAK42151">
        <property type="protein sequence ID" value="AAK42151"/>
        <property type="gene ID" value="SSO1960"/>
    </dbReference>
    <dbReference type="GeneID" id="15296866"/>
    <dbReference type="KEGG" id="sso:SSO1960"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2034"/>
    <dbReference type="eggNOG" id="arCOG01918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_914065_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X00"/>
    <dbReference type="PhylomeDB" id="Q97X00"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37826">
        <property type="entry name" value="FLOTILLIN BAND_7_5 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37826:SF2">
        <property type="entry name" value="ZINC-RIBBON DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="1">
        <location>
            <begin position="42"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="288" mass="31707" checksum="847570B7EC552ED5" modified="2001-10-01" version="1">MSLTIRGQVISTEKEDGSSFMTADAIIFKYPKEAITSKSLIIVQPTERAIVLIQGQIVADLPPGSHNIQTPGNPVSAFLSKFRYNTLPYDTVVYFISTTRHEVRVAGVSQTDDLVPLEYETAIYFRVQNPAALVTNVQFGSLYFKDADLAHYISPIVDQEVSSVLNRVNLTDVFKKFSEISTAVTAALKQFLAEIGVDLISVRITRLLPQDPELRRIIQLRDMGIPLMDAVRMGLARILAEQQNAAAVNMAIGVPYYPNLSTIVNLPDGLIRVGIGRRGQQQSGGAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XA4</accession>
    <name>Q97XA4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1844</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90347">
        <property type="entry name" value="G90347"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923622.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XA4"/>
    <dbReference type="STRING" id="273057.SSO1844"/>
    <dbReference type="PaxDb" id="273057-SSO1844"/>
    <dbReference type="EnsemblBacteria" id="AAK42038">
        <property type="protein sequence ID" value="AAK42038"/>
        <property type="gene ID" value="SSO1844"/>
    </dbReference>
    <dbReference type="GeneID" id="1453365"/>
    <dbReference type="KEGG" id="sso:SSO1844"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1902"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_2_2"/>
    <dbReference type="InParanoid" id="Q97XA4"/>
    <dbReference type="PhylomeDB" id="Q97XA4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="154"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="281"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="376" mass="44530" checksum="050BD2F1CE59D5FE" modified="2001-10-01" version="1">MKLACKIYNTLRWADIYFYQRDGKGLTQTELRQLALDLRKQDDEYKQLYSQVVQQIADRYYEARQRFFEGLARFPKEKKPHKYYSLVYPQYGWKILQVREIRKGSKKNKKRLITLKLSNLGTFKVIIHRDFPLDKVKRVVVKLTRSERIYITFVVEDYEFPKLPNTGKVVAIDVGIEKLIVTSDGEYFPNLRPYEKALWKVKHLHRELSRKKFLSNNWFKAKVKLARAYEHLKNLRTDLYMKLGKWFAEHYDVVVMEDIHVKQLIGKSLRSLRRRLSDVAFSELRDLIKYQLEKYGKKLILVNPAYTSKTCARCGYVKEDLSLSDRVFVCSNCGWIADRDYNASLNILRGSGSERPLVWSSALYQYQHFGTGMAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XB8</accession>
    <name>Q97XB8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90345">
        <property type="entry name" value="H90345"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923613.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XB8"/>
    <dbReference type="PaxDb" id="273057-SSO1826"/>
    <dbReference type="DNASU" id="1453350"/>
    <dbReference type="EnsemblBacteria" id="AAK42023">
        <property type="protein sequence ID" value="AAK42023"/>
        <property type="gene ID" value="SSO1826"/>
    </dbReference>
    <dbReference type="GeneID" id="1453350"/>
    <dbReference type="KEGG" id="sso:SSO1826"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1887"/>
    <dbReference type="eggNOG" id="arCOG06508">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134214_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XB8"/>
    <dbReference type="PhylomeDB" id="Q97XB8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase InsH N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="142" mass="16572" checksum="DE66EED25B2CCCCF" modified="2001-10-01" version="1">MVKAISTEKDLLLKVDKSFPWETFRSKLKSLYSKKPKWDVILLLKVLLIKLIFDISWNNLEGEIRDSKSFMDFLDGKIPPKSTVFSFYKKLQQTVIQEGETMRTTLMDELNKALDKVISEYREKGFELEVGREKTIGSRTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XD1</accession>
    <name>Q97XD1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4064 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90344">
        <property type="entry name" value="C90344"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988458.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XD1"/>
    <dbReference type="PaxDb" id="273057-SSO1813"/>
    <dbReference type="EnsemblBacteria" id="AAK42010">
        <property type="protein sequence ID" value="AAK42010"/>
        <property type="gene ID" value="SSO1813"/>
    </dbReference>
    <dbReference type="GeneID" id="1454789"/>
    <dbReference type="KEGG" id="sso:SSO1813"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1872"/>
    <dbReference type="eggNOG" id="arCOG08436">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2204229_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XD1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="11886" checksum="9C5A381906603CC7" modified="2001-10-01" version="1">MENYQILGLIGSLLLIVGIAIPFAFYYPGYGMMGANGYGMMGRGMMFFGFMGFLVIPAFVLGIVGSLISDKTVAGVLLILASVLSIPAMGLIGVISFILLLIAGILALTSGKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XV7</accession>
    <name>Q97XV7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8459</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90319">
        <property type="entry name" value="E90319"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XV7"/>
    <dbReference type="STRING" id="273057.SSO8459"/>
    <dbReference type="PaxDb" id="273057-SSO8459"/>
    <dbReference type="EnsemblBacteria" id="AAK41812">
        <property type="protein sequence ID" value="AAK41812"/>
        <property type="gene ID" value="SSO8459"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8459"/>
    <dbReference type="HOGENOM" id="CLU_2613803_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XV7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="78" mass="9113" checksum="45BC32514C1417F9" modified="2001-10-01" version="1">MIYLFLLPLPLCHTSYGCKRRLVRGMACNAYIREFYHNVQSDGRGESRPHPKTLLQIVSRPCFPSRGEYYSPRLIKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XX3</accession>
    <name>Q97XX3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1587</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90317">
        <property type="entry name" value="E90317"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923486.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XX3"/>
    <dbReference type="STRING" id="273057.SSO1587"/>
    <dbReference type="PaxDb" id="273057-SSO1587"/>
    <dbReference type="EnsemblBacteria" id="AAK41796">
        <property type="protein sequence ID" value="AAK41796"/>
        <property type="gene ID" value="SSO1587"/>
    </dbReference>
    <dbReference type="GeneID" id="1454575"/>
    <dbReference type="KEGG" id="sso:SSO1587"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1621"/>
    <dbReference type="eggNOG" id="arCOG04756">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079809_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XX3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="303" mass="34295" checksum="7D1B87E4E4EBC619" modified="2001-10-01" version="1">MDIFQLQGLFLVLSAILGYFLHNRLSVRIVSFVNNWINLPLIFLVTYLQRGFEVSDAKIFLTSVIYNFLMFVIVLYLTREIEPFSRGSVLINSIFMNTINLPFSILLAFRGNYEISATVAVAMATLRLPIVMIIFTYLNKGISVIHNTTNNSIIRTNLSRYLPVITFLIGGSLHYVVGSVINVDIVNTINLILILIVLYEFGYQIRRVIGEMRIKDVTSKPYMIIYISRILISPILIFLILISLGITSKIIIEQVMVTAIMAPAITNVVWARVYGFGTEIIVKSTLILTPISTIIAIIFLILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y00</accession>
    <name>Q97Y00_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90314">
        <property type="entry name" value="D90314"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y00"/>
    <dbReference type="STRING" id="273057.SSO1555"/>
    <dbReference type="PaxDb" id="273057-SSO1555"/>
    <dbReference type="EnsemblBacteria" id="AAK41771">
        <property type="protein sequence ID" value="AAK41771"/>
        <property type="gene ID" value="SSO1555"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1555"/>
    <dbReference type="eggNOG" id="arCOG08411">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1674066_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y00"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="157" mass="18701" checksum="B911D8BAC8C1C47A" modified="2001-10-01" version="1">MKRWVNTYYNLEYTLDSMEEEFYYLFLTRDYTSYWVNKFRDVLEKLEEEVSMKQFLSILLYYLFSSSIKFNSLTIQTYSSVLLYFVLGNINFSFASPTGNSRKTRERVKPSITREKQISKEGEKQHKPSRNMGIKTPANRLAVKPLERSNKRGQNPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y61</accession>
    <name>Q97Y61_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second part of one of two inversely orientated ORFs in ISC1043</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90306">
        <property type="entry name" value="G90306"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y61"/>
    <dbReference type="STRING" id="273057.SSO8194"/>
    <dbReference type="PaxDb" id="273057-SSO8194"/>
    <dbReference type="EnsemblBacteria" id="AAK41710">
        <property type="protein sequence ID" value="AAK41710"/>
        <property type="gene ID" value="SSO8194"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8194"/>
    <dbReference type="HOGENOM" id="CLU_2968628_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y61"/>
    <dbReference type="PhylomeDB" id="Q97Y61"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="58" mass="6475" checksum="8CCA7B5993AEA0B8" modified="2001-10-01" version="1">MNAKIPQDLENHLISLPLTLKRITVSDNQVTTQVKDEEGVHEVLPSFISSLDFSLSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y98</accession>
    <name>Q97Y98_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1173</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90301">
        <property type="entry name" value="E90301"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923400.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y98"/>
    <dbReference type="PaxDb" id="273057-SSO1435"/>
    <dbReference type="EnsemblBacteria" id="AAK41668">
        <property type="protein sequence ID" value="AAK41668"/>
        <property type="gene ID" value="SSO1435"/>
    </dbReference>
    <dbReference type="GeneID" id="1454447"/>
    <dbReference type="KEGG" id="sso:SSO1435"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1462"/>
    <dbReference type="eggNOG" id="arCOG03768">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075237_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y98"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009321">
        <property type="entry name" value="DUF973"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06157">
        <property type="entry name" value="DUF973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="312" mass="33521" checksum="81F19196717A665F" modified="2001-10-01" version="1">MSNTNNELQGIRKLRLGLMILMVAGILNLAVTIPRFITPAPSIPSNITANNLNNTNIQQVFTQIASMEIGYFEESIILTSIVGVINIIGLIILRSGFNILSKTVSNEIGIGNIGIIVYIVSYPLIVIGLLLNLFGFLSVNFVEILASLPALFIAIILLIVGSILVGVGIYRVGREYSTNIVKVGGILIAIVIPAFIGSILSYIGLRNVKPITERAREVQIYSIGGGVIKGDGSAQILIYSSVSGSILSVKIEGMNIFTFKVNPATLQPGNNQINIQFDNVSNLVKGSTYIITLLVDVNGNAIEVKTYALYQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YC0</accession>
    <name>Q97YC0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1406</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90298">
        <property type="entry name" value="B90298"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988407.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YC0"/>
    <dbReference type="SMR" id="Q97YC0"/>
    <dbReference type="STRING" id="273057.SSO1406"/>
    <dbReference type="PaxDb" id="273057-SSO1406"/>
    <dbReference type="EnsemblBacteria" id="AAK41641">
        <property type="protein sequence ID" value="AAK41641"/>
        <property type="gene ID" value="SSO1406"/>
    </dbReference>
    <dbReference type="GeneID" id="1454420"/>
    <dbReference type="KEGG" id="sso:SSO1406"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1423"/>
    <dbReference type="eggNOG" id="arCOG01439">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1029036_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YC0"/>
    <dbReference type="PhylomeDB" id="Q97YC0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041165">
        <property type="entry name" value="Cas6_N_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019267">
        <property type="entry name" value="CRISPR-assoc_Cas6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010156">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01877">
        <property type="entry name" value="cas_cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17952">
        <property type="entry name" value="Cas6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10040">
        <property type="entry name" value="CRISPR_Cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Cas6 N-terminal" evidence="7">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="CRISPR-associated protein Cas6 C-terminal" evidence="6">
        <location>
            <begin position="130"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="261" mass="30421" checksum="43CA8E147F157699" modified="2001-10-01" version="1">MIYTLTFRLKPSNDVIIPPFSSKLSRTLFLSFSPTYSKIIESKEPNKPLRITVVKDQGKPLYSNGKSKVVLKAENTYTFIVNTLLEDVVKEVIRVESVNREIYNTSFHVELVNVSVKENVMAEDARFYRVHFKTPTLLQPPRPRMKRKENRYVLFPYVPLLFYSIASHWNRYMDKKIVGVTGSKTLYYFREVNYRIRPMTAYYGNIPNKGFVGWVVFELSARKGSKIRENIRRLLDYVNYFGVGKSRNIGFGEVEVKSLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YF8</accession>
    <name>Q97YF8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1366</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90293">
        <property type="entry name" value="A90293"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YF8"/>
    <dbReference type="PaxDb" id="273057-SSO1366"/>
    <dbReference type="EnsemblBacteria" id="AAK41600">
        <property type="protein sequence ID" value="AAK41600"/>
        <property type="gene ID" value="SSO1366"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1366"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1371"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_8_1_2"/>
    <dbReference type="InParanoid" id="Q97YF8"/>
    <dbReference type="PhylomeDB" id="Q97YF8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="172" mass="20540" checksum="86970DCDB209FC6C" modified="2001-10-01" version="1">MPTLGFRFRAYADEQTLRALKAQLKLACEVYNTLRWADIYFYQRDGKGLTQTELRQLALDLRKQDDEYKQLYSQVVQQIADRYYEAKKRFFEGLARFPKEKKPHKYYSLVYPQYGWKILQVREIRKGSKKNKKKLITLKLSNLGTFKVIVHRDFPLDKVKRVVSGVSPNYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YJ2</accession>
    <name>Q97YJ2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MCM C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90288">
        <property type="entry name" value="H90288"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923324.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YJ2"/>
    <dbReference type="STRING" id="273057.SSO3257"/>
    <dbReference type="PaxDb" id="273057-SSO3257"/>
    <dbReference type="EnsemblBacteria" id="AAK41567">
        <property type="protein sequence ID" value="AAK41567"/>
        <property type="gene ID" value="SSO3257"/>
    </dbReference>
    <dbReference type="GeneID" id="71027430"/>
    <dbReference type="KEGG" id="sso:SSO3257"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1331"/>
    <dbReference type="eggNOG" id="arCOG03169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1500340_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YJ2"/>
    <dbReference type="PhylomeDB" id="Q97YJ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048907">
        <property type="entry name" value="WHD_MCM_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301:SF8">
        <property type="entry name" value="ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301">
        <property type="entry name" value="DNA-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21100">
        <property type="entry name" value="WHD_MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal" evidence="1">
        <location>
            <begin position="113"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="182" mass="20879" checksum="E50DD8394FAB1562" modified="2001-10-01" version="1">MGLLHDFLGVEDPGSPLYGRYYFEITLERFDKEKSRQFLRKGFEELSLKVEENVIQDIIAMVDGIPGWLTLAGNQYFNNRDLKRVKDLAINVALNEINSLISVKNNVSPIVAKRYRTVLRCIAKGMNSWSKILNCVQNEEGSTISTSVLSNILNTLKKLSIIDENYRFLDPVYKEASRMLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YK1</accession>
    <name>Q97YK1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90287">
        <property type="entry name" value="G90287"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923315.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YK1"/>
    <dbReference type="STRING" id="273057.SSO1320"/>
    <dbReference type="PaxDb" id="273057-SSO1320"/>
    <dbReference type="EnsemblBacteria" id="AAK41558">
        <property type="protein sequence ID" value="AAK41558"/>
        <property type="gene ID" value="SSO1320"/>
    </dbReference>
    <dbReference type="GeneID" id="1454337"/>
    <dbReference type="KEGG" id="sso:SSO1320"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1322"/>
    <dbReference type="eggNOG" id="arCOG05914">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978675_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YK1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="283" mass="31266" checksum="2FA7ABA954DEF7E8" modified="2001-10-01" version="1">MNAKIPIILTVIIVVSAFIVVFASTHSTTQENSTADAFHYTTLGEIHTYNISPVTNFILYYVNNTNTTLALSLASQIIGNGTTNFISFNNTFATIGYFKASTPLSSFAFTIINKTLVKNGFIYGQYNTLLYDYKNTTAFGFDGYYFYIIKDNASVNTTLFLLKYLYTSQKVSAPQPSSNVIAYGNTSYGSFQLLSSNSQLILKGTGNFSNVTKLLKYFNITIGNFTVAGKVVYNNATVVIYSISATYDNKSLFVMIGINKQENYGVAVISHQEISLSEILKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YK3</accession>
    <name>Q97YK3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90287">
        <property type="entry name" value="E90287"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923313.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YK3"/>
    <dbReference type="STRING" id="273057.SSO1318"/>
    <dbReference type="PaxDb" id="273057-SSO1318"/>
    <dbReference type="DNASU" id="1454335"/>
    <dbReference type="EnsemblBacteria" id="AAK41556">
        <property type="protein sequence ID" value="AAK41556"/>
        <property type="gene ID" value="SSO1318"/>
    </dbReference>
    <dbReference type="GeneID" id="1454335"/>
    <dbReference type="KEGG" id="sso:SSO1318"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1320"/>
    <dbReference type="eggNOG" id="arCOG02441">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1006930_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YK3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37305">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37305:SF1">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="31025" checksum="164FA24DF8AFBFC6" modified="2001-10-01" version="1">MFEIMLYEWRRAIARKKVIVLSIITFIFELGIYLAIYLVPSKSLKTLIIPLSPYLWALGVLLPQVLLIHFLAISIASGSMAEEYEQGTVDYFISKPITRLRFVMEKWLGSFSLLLIIYLFMVVLALVLSSLLFGVQSDLILLPELFSSVVFSTLVFLNIAFAIGEILRRSNLSFTISGFILIASIIVSDVLVFVSLLTNNRSYEMISEYLPTWGSTELPFMLLQNSPFSTIARGLNILPSISNNVGLAIVSIVVYSLVPLVLSIINFLGRDIPKKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YM8</accession>
    <name>Q97YM8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CobQ/CobB/MinD/ParA nucleotide binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90284">
        <property type="entry name" value="E90284"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991984.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YM8"/>
    <dbReference type="SMR" id="Q97YM8"/>
    <dbReference type="STRING" id="273057.SSO1294"/>
    <dbReference type="PaxDb" id="273057-SSO1294"/>
    <dbReference type="EnsemblBacteria" id="AAK41532">
        <property type="protein sequence ID" value="AAK41532"/>
        <property type="gene ID" value="SSO1294"/>
    </dbReference>
    <dbReference type="GeneID" id="1454311"/>
    <dbReference type="KEGG" id="sso:SSO1294"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1296"/>
    <dbReference type="eggNOG" id="arCOG07293">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090891_1_0_2"/>
    <dbReference type="InParanoid" id="Q97YM8"/>
    <dbReference type="PhylomeDB" id="Q97YM8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF10">
        <property type="entry name" value="ATPASE INVOLVED IN CHROMOSOME PARTITIONING, PARA_MIND FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="1">
        <location>
            <begin position="7"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="247" mass="28007" checksum="719FCD240EABEBD1" modified="2001-10-01" version="1">MKIRVDIIGLKGGVGKTTIALNTALYLSKRYKVLYIDKDLLSMGSLILGFNGLGFNKAIVEGLGNEQYEYKVNNNLTLFKLYSDPVNERELQHKVKGMGEKAERAYVDVVSKGYDVIIVDYGRIFRTNDPLVYDEYEMFKKNFPDYTIAAAGITDAIRNDVTDVVKYFLDTINRIKAKPLAFVINMVPQIGDIQKEINQIVREISEVVKCDILTIPFNEKLVQYANMGEVEEMQKVGKLIERILSNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YN1</accession>
    <name>Q97YN1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in partial transposon ISC1160</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO7607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK67514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YN1"/>
    <dbReference type="STRING" id="273057.SSO7607"/>
    <dbReference type="PaxDb" id="273057-SSO7607"/>
    <dbReference type="EnsemblBacteria" id="AAK67514">
        <property type="protein sequence ID" value="AAK67514"/>
        <property type="gene ID" value="SSO7607"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7607"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1292"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113529_1_0_2"/>
    <dbReference type="InParanoid" id="Q97YN1"/>
    <dbReference type="PhylomeDB" id="Q97YN1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025471">
        <property type="entry name" value="DUF4322"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14210">
        <property type="entry name" value="DUF4322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4322" evidence="1">
        <location>
            <begin position="2"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK67514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="82" mass="9191" checksum="1737C3011FD446A7" modified="2001-10-01" version="1">MITLDFPHRNDVEQIGHKLLSMIDFKGSKVEEVAKVLVSAALLNDSIENKLKMFGISDQTVSNYAEKEEVAERTRKSKAIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YN8</accession>
    <name>Q97YN8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial transposase ISC1250</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90283">
        <property type="entry name" value="E90283"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YN8"/>
    <dbReference type="STRING" id="273057.SSO1286"/>
    <dbReference type="PaxDb" id="273057-SSO1286"/>
    <dbReference type="EnsemblBacteria" id="AAK41524">
        <property type="protein sequence ID" value="AAK41524"/>
        <property type="gene ID" value="SSO1286"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1286"/>
    <dbReference type="eggNOG" id="arCOG04918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174524_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YN8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="108" mass="12540" checksum="C02B947645B10FEA" modified="2001-10-01" version="1">MIFNFAQIGQRHVIISSAELGGIKSEANPTLLSYLVASKELWKFKTNNLVESFNSLLERRFSKFHSPWRILQIARAIALYYNLLTYFLTIIIILQLPSFLSISQKYIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YN9</accession>
    <name>Q97YN9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1285</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90283">
        <property type="entry name" value="D90283"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923288.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YN9"/>
    <dbReference type="STRING" id="273057.SSO1285"/>
    <dbReference type="PaxDb" id="273057-SSO1285"/>
    <dbReference type="EnsemblBacteria" id="AAK41523">
        <property type="protein sequence ID" value="AAK41523"/>
        <property type="gene ID" value="SSO1285"/>
    </dbReference>
    <dbReference type="GeneID" id="1454302"/>
    <dbReference type="KEGG" id="sso:SSO1285"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1285"/>
    <dbReference type="eggNOG" id="arCOG09948">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154343_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YN9"/>
    <dbReference type="PhylomeDB" id="Q97YN9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="145" mass="16570" checksum="013C74A3164B8A8D" modified="2001-10-01" version="1">MRFNDVFLLMGYTTSKLKNFTAFRKYKGSWGGKHGKSYFGFKVCNLVERRTNFVRDFKVGLLSDLAFSFDNVKLMADRAWISKKDVLVKGVGSARLPVEGSGVKIREGKASSTTLRGVVMEVFFLNFYRDLEILSTRIRTKVFVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-01-24" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YX2</accession>
    <name>Q97YX2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90271">
        <property type="entry name" value="E90271"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YX2"/>
    <dbReference type="STRING" id="273057.SSO1180"/>
    <dbReference type="PaxDb" id="273057-SSO1180"/>
    <dbReference type="EnsemblBacteria" id="AAK41428">
        <property type="protein sequence ID" value="AAK41428"/>
        <property type="gene ID" value="SSO1180"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1180"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1176"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YX2"/>
    <dbReference type="PhylomeDB" id="Q97YX2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="14"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="229" mass="27133" checksum="CAB066180B1960A9" modified="2001-10-01" version="1">MGEKEQEKAEGFLLYNWKRKGIKMRSLNLVYPLRLPLLVEVADLRSDSPSQFLLRSVMEVSQYMEIDYVVADAGFLNLGVIKEMPVKTIVRGKSNLKGFKELSNVPLVEKRYEVKDRVYVAYRVLKFEGLYYYDVVYVKGKPRHFIFVTNFEGDPYELAELYRLRWQVEEGFKVRKARIRYVRKLSNKIFLFLYYTVLDSAWNLVNHLLFNFKSTCKKGLSFDLFVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YZ6</accession>
    <name>Q97YZ6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase (Acd-1)</fullName>
            <ecNumber evidence="10">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">acd-1</name>
        <name evidence="10" type="ordered locus">SSO1153</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="10"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90268">
        <property type="entry name" value="E90268"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991592.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YZ6"/>
    <dbReference type="STRING" id="273057.SSO1153"/>
    <dbReference type="PaxDb" id="273057-SSO1153"/>
    <dbReference type="EnsemblBacteria" id="AAK41404">
        <property type="protein sequence ID" value="AAK41404"/>
        <property type="gene ID" value="SSO1153"/>
    </dbReference>
    <dbReference type="GeneID" id="1454183"/>
    <dbReference type="KEGG" id="sso:SSO1153"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1147"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_9_0_2"/>
    <dbReference type="InParanoid" id="Q97YZ6"/>
    <dbReference type="PhylomeDB" id="Q97YZ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000011">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052161">
        <property type="entry name" value="Mycobact_Acyl-CoA_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43292">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43292:SF4">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE FADE34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="33"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="125"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="270"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK41404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="363" mass="41474" checksum="B6B6913D27768BDB" modified="2001-10-01" version="1">MNEEEYRLKLREWIRNSAPESLKGRKILFETVEFDDYNELRSWQRKLADAGYLGITWPKEYGGQGLDPIYEVIAYEEFIRAGLPYGRSLGSIGLMVVAPAILKHGNEDQKRRYLPRILRAEDIWCQGFSEPQAGSDLASVTTRAEDKGDYFLVNGQKIWSSYAHIADYMILLARTGEDKYKGLTMFILNMKQEGIRVSPINQLTGKSDFNTVYFNNVKVPKNNVIGNVGDGWKVAMTVLNHERFMLGVTMLFTSKIALESLKGVKEKEELEDEVEGLEAFYRRILIKLRKGEDIDVEGSMLKLLASELLQRIYEIAVMNYDLETIMKEKWYLGMLASRGRTIAGGTSEILRNVVGERALKLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z13</accession>
    <name>Q97Z13_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90266">
        <property type="entry name" value="B90266"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z13"/>
    <dbReference type="STRING" id="273057.SSO1133"/>
    <dbReference type="PaxDb" id="273057-SSO1133"/>
    <dbReference type="EnsemblBacteria" id="AAK41385">
        <property type="protein sequence ID" value="AAK41385"/>
        <property type="gene ID" value="SSO1133"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1133"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1125"/>
    <dbReference type="eggNOG" id="arCOG05916">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102440_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z13"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="203" mass="23455" checksum="10B8A51482244FAA" modified="2001-10-01" version="1">MQMKTDFLNSLEINTEEDVKKLFDVIFYAKKHYSEIIGNNGIDKVKLAFKTLKNKDLPYDERVKAFTSLKASEPEDIEDMAKEIIHFLEPEKYPLWTRWVWNPSKNSGSITYVLKDGVVLKNEKEYFDAVSELREVLSIFGLDSPNYYYTSIFLVYSYVRYVDYATLLAVDRKGGGLYPSHLSTTAMVLGLKSFLRVIQLANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z72</accession>
    <name>Q97Z72_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90258">
        <property type="entry name" value="B90258"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z72"/>
    <dbReference type="STRING" id="273057.SSO1057"/>
    <dbReference type="PaxDb" id="273057-SSO1057"/>
    <dbReference type="EnsemblBacteria" id="AAK41321">
        <property type="protein sequence ID" value="AAK41321"/>
        <property type="gene ID" value="SSO1057"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1057"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1053"/>
    <dbReference type="eggNOG" id="arCOG05921">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172999_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z72"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="114" mass="13361" checksum="1E5CA0930401DDE4" modified="2001-10-01" version="1">MGILMTVEESILGTGERERREIVGYIQMLLDSINDLMVKYKQELKNMGVINRLGILTEIITMHKYNPEVYMGNYWEELLSLINIIKQDQKLANEVKDIEELIEKINSLKELVKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z83</accession>
    <name>PRIS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA primase small subunit PriS</fullName>
            <ecNumber evidence="1">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">priS</name>
        <name type="synonym">priA</name>
        <name type="ordered locus">SSO1048</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="J. Mol. Biol." volume="344" first="1251" last="1263">
            <title>The heterodimeric primase of the hyperthermophilic archaeon Sulfolobus solfataricus possesses DNA and RNA primase, polymerase and 3'-terminal nucleotidyl transferase activities.</title>
            <authorList>
                <person name="Lao-Sirieix S.H."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="15561142"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2004.10.018"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>GENE NAME</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2005" name="Nat. Struct. Mol. Biol." volume="12" first="1137" last="1144">
            <title>Structure of the heterodimeric core primase.</title>
            <authorList>
                <person name="Lao-Sirieix S.H."/>
                <person name="Nookala R.K."/>
                <person name="Roversi P."/>
                <person name="Bell S.D."/>
                <person name="Pellegrini L."/>
            </authorList>
            <dbReference type="PubMed" id="16273105"/>
            <dbReference type="DOI" id="10.1038/nsmb1013"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.33 ANGSTROMS) IN COMPLEX WITH ZINC</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF PHE-164 AND GLY-165</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2006" name="EMBO Rep." volume="7" first="539" last="545">
            <title>GINS, a central nexus in the archaeal DNA replication fork.</title>
            <authorList>
                <person name="Marinsek N."/>
                <person name="Barry E.R."/>
                <person name="Makarova K.S."/>
                <person name="Dionne I."/>
                <person name="Koonin E.V."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="16485022"/>
            <dbReference type="DOI" id="10.1038/sj.embor.7400649"/>
        </citation>
        <scope>INTERACTION WITH GINS15 AND PRIL</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair. Possesses a template-independent 3'-terminal nucleotidyl transferase activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3 4">Heterodimer of a small subunit (PriS) and a large subunit (PriL) (PubMed:15561142, PubMed:16485022, PubMed:16273105). Interacts with Gins23 (PubMed:16485022).</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-8081502">
            <id>Q97Z83</id>
        </interactant>
        <interactant intactId="EBI-8081454">
            <id>Q9UWW1</id>
            <label>priL</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>5</experiments>
    </comment>
    <comment type="miscellaneous">
        <text evidence="5">The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA (PubMed:16273105).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90256">
        <property type="entry name" value="G90256"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989180.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1ZT2">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.33 A"/>
        <property type="chains" value="A/C=1-330"/>
    </dbReference>
    <dbReference type="PDB" id="5OF3">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.91 A"/>
        <property type="chains" value="A/D=1-330"/>
    </dbReference>
    <dbReference type="PDB" id="5OFN">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="A=1-330"/>
    </dbReference>
    <dbReference type="PDBsum" id="1ZT2"/>
    <dbReference type="PDBsum" id="5OF3"/>
    <dbReference type="PDBsum" id="5OFN"/>
    <dbReference type="AlphaFoldDB" id="Q97Z83"/>
    <dbReference type="SMR" id="Q97Z83"/>
    <dbReference type="DIP" id="DIP-42889N"/>
    <dbReference type="FunCoup" id="Q97Z83">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="IntAct" id="Q97Z83">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="MINT" id="Q97Z83"/>
    <dbReference type="STRING" id="273057.SSO1048"/>
    <dbReference type="PaxDb" id="273057-SSO1048"/>
    <dbReference type="EnsemblBacteria" id="AAK41310">
        <property type="protein sequence ID" value="AAK41310"/>
        <property type="gene ID" value="SSO1048"/>
    </dbReference>
    <dbReference type="GeneID" id="1454089"/>
    <dbReference type="GeneID" id="44129977"/>
    <dbReference type="KEGG" id="sso:SSO1048"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1042"/>
    <dbReference type="eggNOG" id="arCOG04110">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056123_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z83"/>
    <dbReference type="PhylomeDB" id="Q97Z83"/>
    <dbReference type="BRENDA" id="2.7.7.102">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="2.7.7.B16">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97Z83"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.920.10:FF:000006">
        <property type="entry name" value="DNA primase small subunit PriS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00700">
        <property type="entry name" value="DNA_primase_sml_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
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        <property type="entry name" value="PRK00419.1-3"/>
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    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
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        <property type="entry name" value="DNA_primase_S"/>
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    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0639">Primosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
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            <begin position="1"/>
            <end position="330"/>
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    <feature type="active site" evidence="1">
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    <feature type="active site" evidence="1">
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    <feature type="binding site" evidence="3">
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
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    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="119"/>
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        <ligand>
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        <ligand>
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    <feature type="binding site" evidence="3">
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Strong decrease in interaction with PriL." evidence="3">
        <original>F</original>
        <variation>E</variation>
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Partial destabilization of PriS-PriL complex." evidence="3">
        <original>G</original>
        <variation>I</variation>
        <location>
            <position position="165"/>
        </location>
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    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15561142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="16273105"/>
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    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="16485022"/>
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    </evidence>
    <evidence type="ECO:0000305" key="5">
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            <dbReference type="PubMed" id="16273105"/>
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    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="1ZT2"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="5OF3"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="5OFN"/>
        </source>
    </evidence>
    <sequence length="330" mass="37598" checksum="E9B3C556E619B66E" modified="2001-10-01" version="1">MGTFTLHQGQTNLIKSFFRNYYLNAELELPKDMELREFALQPFGSDTYVRHLSFSSSEELRDYLVNRNLPLHLFYSSARYQLPSARNMEEKAWMGSDLLFDIDADHLCKLRSIRFCPVCGNAVVSEKCERDNVETLEYVEMTSECIKRGLEQTRNLVEILEDDFGLKPKVYFSGNRGFHVQVDCYGNCALLDSDERKEIAEYVMGIGVPGYPGGSENAPGWVGRKNRGINGVTIDEQVTIDVKRLIRIPNSLHGKSGLIVKRVPNLDDFEFNETLSPFTGYTIFLPYITIETEVLGSIIKLNRGIPIKIKSSIGIYLHLRNLGEVKAYVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZB5</accession>
    <name>Q97ZB5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid specific permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1009</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="AA/rel_permease1"/>
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        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
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        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="468" mass="50231" checksum="478E867E4195DB6B" modified="2001-10-01" version="1">MDEEKKNVSELRKGVLGTWLVASYGIAANAPIAVATLYFVGLAGLVGGAMPLTVILSYLIYATTLIVIYEWSKEIAASYGYVAMIKKGLGSSLASFTVGYGYIYQYLVAGTAGFGILGIASFIYLISPSIASSMPWLWAAIVIIVTIEITTIMWLGVKPGGLLNLVIGLISIGFLIITSIVLIAGAKNSILPFTAVPVNNNWALVLTAMIFGVTTFGGATTPIGVAEEAKVPKSTLPKALLLTFGILGVGLILNSYAQTIVYGINNMFNYANLPDPMIVIYSKYFNPAIVYMLIILVAFMFNSSALAFATSGSRMIFGMARDGVLYPKVFSKVNKYGVPGNAIILTGIVTGALSLISGYILGPLEASIFLITFGSFYVALGHLFAALGLIVRKVKMRTANIAKHVVIPIISILLYIAVIYFGTYPAPAFPLNIAVYAAWAILLIHIITYYVIKSRFPDRIKKFGDYSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2026-01-28" version="144" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZF5</accession>
    <name>RGYR2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 12">Reverse gyrase 2</fullName>
            <shortName evidence="16">RG2</shortName>
            <ecNumber evidence="1 8 9">5.6.2.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">TopR2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rgy2</name>
        <name evidence="11 13 14" type="synonym">topR-2</name>
        <name type="ordered locus">SSO0963</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="J. Biol. Chem." volume="279" first="33192" last="33198">
            <title>Reverse gyrase recruitment to DNA after UV light irradiation in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Napoli A."/>
                <person name="Valenti A."/>
                <person name="Salerno V."/>
                <person name="Nadal M."/>
                <person name="Garnier F."/>
                <person name="Rossi M."/>
                <person name="Ciaramella M."/>
            </authorList>
            <dbReference type="PubMed" id="15190074"/>
            <dbReference type="DOI" id="10.1074/jbc.m402619200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>REACTION MECHANISM</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="J. Virol." volume="82" first="4874" last="4883">
            <title>Transcriptome analysis of infection of the archaeon Sulfolobus solfataricus with Sulfolobus turreted icosahedral virus.</title>
            <authorList>
                <person name="Ortmann A.C."/>
                <person name="Brumfield S.K."/>
                <person name="Walther J."/>
                <person name="McInnerney K."/>
                <person name="Brouns S.J."/>
                <person name="van de Werken H.J."/>
                <person name="Bothner B."/>
                <person name="Douglas T."/>
                <person name="van de Oost J."/>
                <person name="Young M.J."/>
            </authorList>
            <dbReference type="PubMed" id="18337566"/>
            <dbReference type="DOI" id="10.1128/jvi.02583-07"/>
        </citation>
        <scope>INDUCTION BY VIRUS (MICROBIAL INFECTION)</scope>
        <source>
            <strain>2-2-12</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2006" name="Nucleic Acids Res." volume="34" first="2098" last="2108">
            <title>Selective degradation of reverse gyrase and DNA fragmentation induced by alkylating agent in the archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Valenti A."/>
                <person name="Napoli A."/>
                <person name="Ferrara M.C."/>
                <person name="Nadal M."/>
                <person name="Rossi M."/>
                <person name="Ciaramella M."/>
            </authorList>
            <dbReference type="PubMed" id="16617150"/>
            <dbReference type="DOI" id="10.1093/nar/gkl115"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>INDUCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2008" name="Extremophiles" volume="12" first="799" last="809">
            <title>Transcriptional analysis of the two reverse gyrase encoding genes of Sulfolobus solfataricus P2 in relation to the growth phases and temperature conditions.</title>
            <authorList>
                <person name="Garnier F."/>
                <person name="Nadal M."/>
            </authorList>
            <dbReference type="PubMed" id="18777006"/>
            <dbReference type="DOI" id="10.1007/s00792-008-0186-2"/>
        </citation>
        <scope>INDUCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2011" name="J. Mol. Biol." volume="408" first="839" last="849">
            <title>TopR2, the second reverse gyrase of Sulfolobus solfataricus, exhibits unusual properties.</title>
            <authorList>
                <person name="Bizard A."/>
                <person name="Garnier F."/>
                <person name="Nadal M."/>
            </authorList>
            <dbReference type="PubMed" id="21435345"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2011.03.030"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>REACTION MECHANISM</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2020" name="Mol. Microbiol." volume="113" first="356" last="368">
            <title>The reverse gyrase TopR1 is responsible for the homeostatic control of DNA supercoiling in the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Couturier M."/>
                <person name="Gadelle D."/>
                <person name="Forterre P."/>
                <person name="Nadal M."/>
                <person name="Garnier F."/>
            </authorList>
            <dbReference type="PubMed" id="31713907"/>
            <dbReference type="DOI" id="10.1111/mmi.14424"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PROTEIN ABUNDANCE</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>INDUCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2020" name="Proc. Natl. Acad. Sci. U.S.A." volume="117" first="10856" last="10864">
            <title>Direct observation of helicase-topoisomerase coupling within reverse gyrase.</title>
            <authorList>
                <person name="Yang X."/>
                <person name="Garnier F."/>
                <person name="Debat H."/>
                <person name="Strick T.R."/>
                <person name="Nadal M."/>
            </authorList>
            <dbReference type="PubMed" id="32371489"/>
            <dbReference type="DOI" id="10.1073/pnas.1921848117"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>REACTION MECHANISM</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>ACTIVE SITE</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>DNA-BINDING</scope>
        <scope>MUTAGENESIS OF TYR-903</scope>
    </reference>
    <comment type="function">
        <text evidence="6 8 9 10 18 19 20">Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process (Probable) (PubMed:15190074, PubMed:16617150, PubMed:18337566, PubMed:31713907, PubMed:32371489). A highly processive enzyme, it introduces a large number of positive supercoils directly in a negatively supercoiled substrate (PubMed:21435345). At 75 degrees Celsius introduces more than 23 positive supercoils into pTZ18R DNA (probably 2860 bp), more than TopR1; unlike TopR1 little to no relaxation of the negatively supercoiled substrate is seen in the presence of ATP, in the absence of ATP no activity is seen (PubMed:21435345). At 45 degrees Celsius the enzyme is slower and in vitro individual steps can be detected (PubMed:32371489). It cleaves transiently a single DNA strand and remains covalently bound to the 5' DNA end through a tyrosine residue (Probable) (PubMed:32371489). May be involved in DNA damage response (Probable) (PubMed:15190074). May be involved in rewinding the DNA strands in the regions of the chromosome that have opened up to allow transcription or replication.</text>
    </comment>
    <comment type="function">
        <text evidence="9 18 19">There are 2 genes for this protein in the cell. During exponential growth this is the more highly expressed isoform (about 125 molecules per cell at 80 degrees Celsius, about 117 molecules at 88 degrees Celsius); this isoform is less active at higher temperature (PubMed:31713907). Grows actively at both 80 and 88 degrees Celsius; survives a long exposure at 45 degrees Celsius without DNA replication or cell division occurring (PubMed:31713907). Experiments using whole cell extracts do not distinguish which isoform is present, the results are probably a mixture of the two forms (Probable) (PubMed:15190074, PubMed:16617150).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 zinc ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="8 18">At least one of the 2 proteins is inhibited by actinomycin D (Probable) (PubMed:15190074). Less sensitive to NaCl than TopR1, maximal positive supercoiling is observed with 100 mM NaCl; as NaCl rises higher than 400 mM supercoiling decreases (PubMed:21435345). At 600 mM NaCl relaxes but does not introduce positive supercoils into negatively supercoiled substrate (PubMed:21435345).</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="10">1 uM for ATP with negatively supercoiled DNA substrate at 45 degrees Celsius</KM>
            <KM evidence="10">7.1 uM for ATP with positively supercoiled DNA substrate at 45 degrees Celsius</KM>
        </kinetics>
        <temperatureDependence>
            <text evidence="8 9">Optimum temperature is 70-75 degrees Celsius, between 60 and 80 degrees Celsius high levels of positive supercoils are seen in vitro (PubMed:21435345). Active at 80 degrees, much less active at 88 degrees Celsius in vitro (PubMed:31713907).</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="1 10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="18">Upon UV irradiation up to 60% of the reverse gyrase activity becomes associated with DNA in vivo (PubMed:15190074).</text>
    </comment>
    <comment type="induction">
        <text evidence="5 7 9 19">No change in protein levels during shifts from 80 to 88 or 88 to 80 degrees Celsius (at protein level) (PubMed:31713907). Constitutively transcribed during cell growth; no change is seen in the presence of methyl methanesulfonate (MMS) (PubMed:16617150). 0.7 mM MMS reduces the amount of enzyme at a post-translational level via a metalloprotease (at protein level) (Probable) (PubMed:16617150). At 72 degrees Celsius this gene is transcribed at low levels during early and late exponential growth (until about 50 hours), declines as stationary phase starts, transcripts never disappear completely (PubMed:18777006). At 80 degrees Celsius more transcripts accumulate with maximal levels at about 70 hours, declines more rapidly than at 72 degrees (PubMed:18777006). No change in transcript levels is seen when cells shift from 72 to 80, or 80 to 72 degrees Celsius (PubMed:18777006).</text>
    </comment>
    <comment type="induction">
        <text evidence="6">(Microbial infection) This isoform is induced at least 4-fold following infection by Sulfolobus turreted icosahedral virus 1 (STIV-1) in strain 2-2-12 (PubMed:18337566).</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Both the DNA unwinding and positive supercoiling activities require the cooperation of both domains. The cooperative action between the helicase-like and the topoisomerase domains is specific. The helicase-like domain probably does not directly unwind DNA but acts more likely by driving ATP-dependent conformational changes within the whole enzyme, functioning more like a protein motor. The 'latch' region of the N-terminal domain plays a regulatory role in the enzyme, repressing topoisomerase activity in the absence of ATP and therefore preventing the enzyme from acting as an ATP-independent relaxing enzyme; it also helps to coordinate nucleotide hydrolysis by the ATPase domain with the supercoiling activity of the topoisomerase domain.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">This enzyme is the only unique feature of hyperthermophilic bacteria/archaea known and seems to be essential for adaptation to life at high temperatures. It may play a role in stabilization of DNA at high temperatures.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">Strain 2-2-12 is a substrain of P2 that is highly susceptible to infection by Sulfolobus turreted icosahedral virus 1 (STIV-1).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the DEAD box helicase family. DDVD subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Ala-192 is present instead of the conserved Val which is part of the DDVD box.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.-" evidence="1 8 9"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90247">
        <property type="entry name" value="F90247"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992408.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZF5"/>
    <dbReference type="SMR" id="Q97ZF5"/>
    <dbReference type="FunCoup" id="Q97ZF5">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0963"/>
    <dbReference type="PaxDb" id="273057-SSO0963"/>
    <dbReference type="EnsemblBacteria" id="AAK41237">
        <property type="protein sequence ID" value="AAK41237"/>
        <property type="gene ID" value="SSO0963"/>
    </dbReference>
    <dbReference type="GeneID" id="1455204"/>
    <dbReference type="KEGG" id="sso:SSO0963"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.961"/>
    <dbReference type="eggNOG" id="arCOG01526">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_002886_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZF5"/>
    <dbReference type="PhylomeDB" id="Q97ZF5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
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    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160097">
        <property type="term" value="F:reverse gyrase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd17924">
        <property type="entry name" value="DDXDc_reverse_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18798">
        <property type="entry name" value="SF2_C_reverse_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03361">
        <property type="entry name" value="TOPRIM_TopoIA_RevGyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.510.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01125">
        <property type="entry name" value="Reverse_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005736">
        <property type="entry name" value="Reverse_gyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034142">
        <property type="entry name" value="TOPRIM_RevGyr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040569">
        <property type="entry name" value="Znf_Rg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01054">
        <property type="entry name" value="rgy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43505">
        <property type="entry name" value="REVERSE GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43505:SF1">
        <property type="entry name" value="REVERSE GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17915">
        <property type="entry name" value="zf_Rg"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
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        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52037">
        <property type="entry name" value="ZF_RG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52036">
        <property type="entry name" value="ZF_RG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0799">Topoisomerase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000158093" description="Reverse gyrase 2">
        <location>
            <begin position="1"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="96"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="1">
        <location>
            <begin position="580"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="4">
        <location>
            <begin position="759"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="RG N-terminal-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="RG C-terminal-type" evidence="3">
        <location>
            <begin position="662"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Topoisomerase I" evidence="1">
        <location>
            <begin position="576"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEAD box" evidence="1">
        <location>
            <begin position="190"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="4 20">
        <location>
            <position position="903"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="109"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="586"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="665"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="668"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="679"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="682"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="712"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Binds but cannot cleave DNA." evidence="10">
        <original>Y</original>
        <variation>F</variation>
        <location>
            <position position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="16617150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="18337566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="18777006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="8">
        <source>
            <dbReference type="PubMed" id="21435345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="9">
        <source>
            <dbReference type="PubMed" id="31713907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="10">
        <source>
            <dbReference type="PubMed" id="32371489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="11">
        <source>
            <dbReference type="PubMed" id="11427726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="12">
        <source>
            <dbReference type="PubMed" id="15190074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="13">
        <source>
            <dbReference type="PubMed" id="16617150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="14">
        <source>
            <dbReference type="PubMed" id="18337566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="15">
        <source>
            <dbReference type="PubMed" id="21435345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="16">
        <source>
            <dbReference type="PubMed" id="32371489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="17"/>
    <evidence type="ECO:0000305" key="18">
        <source>
            <dbReference type="PubMed" id="15190074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="19">
        <source>
            <dbReference type="PubMed" id="16617150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="20">
        <source>
            <dbReference type="PubMed" id="32371489"/>
        </source>
    </evidence>
    <sequence length="1166" mass="132077" checksum="F3929256A59F791D" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZJ5</accession>
    <name>Q97ZJ5_SACS2</name>
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        <recommendedName>
            <fullName evidence="3">CdvA-like coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0911</name>
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    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
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        <lineage>
            <taxon>Archaea</taxon>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Mol. Cell" volume="41" first="186" last="196">
            <title>Molecular and structural basis of ESCRT-III recruitment to membranes during archaeal cell division.</title>
            <authorList>
                <person name="Samson R.Y."/>
                <person name="Obita T."/>
                <person name="Hodgson B."/>
                <person name="Shaw M.K."/>
                <person name="Chong P.L."/>
                <person name="Williams R.L."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="21255729"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2010.12.018"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 251-265</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="B90242"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923151.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2XVC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.15 A"/>
        <property type="chains" value="B=251-265"/>
    </dbReference>
    <dbReference type="PDBsum" id="2XVC"/>
    <dbReference type="AlphaFoldDB" id="Q97ZJ5"/>
    <dbReference type="SMR" id="Q97ZJ5"/>
    <dbReference type="STRING" id="273057.SSO0911"/>
    <dbReference type="PaxDb" id="273057-SSO0911"/>
    <dbReference type="EnsemblBacteria" id="AAK41193">
        <property type="protein sequence ID" value="AAK41193"/>
        <property type="gene ID" value="SSO0911"/>
    </dbReference>
    <dbReference type="GeneID" id="1455160"/>
    <dbReference type="KEGG" id="sso:SSO0911"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.913"/>
    <dbReference type="eggNOG" id="arCOG04054">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100857_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZJ5"/>
    <dbReference type="PhylomeDB" id="Q97ZJ5"/>
    <dbReference type="EvolutionaryTrace" id="Q97ZJ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="CdvA_CC"/>
    </dbReference>
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        <property type="entry name" value="Cell_Div_Membrane-Interact"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041007">
        <property type="entry name" value="cell_div_CdvA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CdvA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="6" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CdvA-like coiled-coil" evidence="3">
        <location>
            <begin position="113"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="104"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="2XVC"/>
        </source>
    </evidence>
    <sequence length="265" mass="30541" checksum="38750C2C1E6F49A4" modified="2001-10-01" version="1">MTNLRYLKSPNLYKYYLKDQIFLRSITMPVSYEVLTKFIGQKVKDIYGREFGYLIHVYSEIDGSITGIEVAQGSSIMTMGPERIKVDGDSILILPDWKAEALRILSLMEKIRKRQRALEELYNKQEIPKSDYDEMKRKLDTEMLKLKDDQNKLKGKLKSRLNDIEDQLAHIDKAVISLKMSYISSEIPENAYKGSIEVLRQSKDSYTLERDDIRKTLDRLDSLDKESIELKPLGSLSTSQQGEVKSDQSKSEIPLPIPVKVINTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZK3</accession>
    <name>Q97ZK3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0903</name>
    </gene>
    <organism evidence="2 3">
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        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90241">
        <property type="entry name" value="B90241"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992327.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZK3"/>
    <dbReference type="STRING" id="273057.SSO0903"/>
    <dbReference type="PaxDb" id="273057-SSO0903"/>
    <dbReference type="EnsemblBacteria" id="AAK41185">
        <property type="protein sequence ID" value="AAK41185"/>
        <property type="gene ID" value="SSO0903"/>
    </dbReference>
    <dbReference type="GeneID" id="1455152"/>
    <dbReference type="KEGG" id="sso:SSO0903"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.905"/>
    <dbReference type="eggNOG" id="arCOG07219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1544266_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZK3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="173" mass="19513" checksum="DB7BE90B29994ECB" modified="2001-10-01" version="1">MNLASYYKLEPLKINDIVVLYTLRKTKEAEIIDGIRDLESIIPDESNDVVIIEGDNEKYINGKFKLDFQFSRPLNVISIGVPQVVNGDQIQITSIDVNMGNIIGGVLVKRDLAFLMFYTYDGTKIISMSDINPPIPEKKESVKRVKKKRKRSKKRAKKSSSKSKSKSKSSGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZL3</accession>
    <name>Q97ZL3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0881</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90238">
        <property type="entry name" value="H90238"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992298.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZL3"/>
    <dbReference type="FunCoup" id="Q97ZL3">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0881"/>
    <dbReference type="PaxDb" id="273057-SSO0881"/>
    <dbReference type="EnsemblBacteria" id="AAK41167">
        <property type="protein sequence ID" value="AAK41167"/>
        <property type="gene ID" value="SSO0881"/>
    </dbReference>
    <dbReference type="GeneID" id="15297694"/>
    <dbReference type="KEGG" id="sso:SSO0881"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.888"/>
    <dbReference type="eggNOG" id="arCOG00452">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095961_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZL3"/>
    <dbReference type="PhylomeDB" id="Q97ZL3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053492">
        <property type="entry name" value="Cell_Div_Machinery_Comp"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041005">
        <property type="entry name" value="cell_div_CdvB1_B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="221" mass="25036" checksum="5642FB3456D11DB5" modified="2001-10-01" version="1">MASSKVEDFVKNWGGKQEPSIGERIKNAFKPQQPLRYRLVMANYRLRTMVSRLDVYISRLQERDRTLFEKVVESQMSKDTARAAMYANEIAEIRKISRQLITTQIALEQVQLRLETITELGDVFNSLIPVLGVIKELRNAMKGVMPEISLELAELEEGLQEVVIEAGDFTGAPANYGASSPEARKILEEASVVAEQRMKEKFPELPSFVTSTQKVSNQEQK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2025-06-18" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZN1</accession>
    <name>SYR_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="1">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argS</name>
        <name type="ordered locus">SSO0857</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90236">
        <property type="entry name" value="E90236"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923143.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZN1"/>
    <dbReference type="SMR" id="Q97ZN1"/>
    <dbReference type="FunCoup" id="Q97ZN1">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0857"/>
    <dbReference type="PaxDb" id="273057-SSO0857"/>
    <dbReference type="EnsemblBacteria" id="AAK41148">
        <property type="protein sequence ID" value="AAK41148"/>
        <property type="gene ID" value="SSO0857"/>
    </dbReference>
    <dbReference type="GeneID" id="7806735"/>
    <dbReference type="KEGG" id="sso:SSO0857"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.869"/>
    <dbReference type="eggNOG" id="arCOG00487">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006406_6_1_2"/>
    <dbReference type="InParanoid" id="Q97ZN1"/>
    <dbReference type="PhylomeDB" id="Q97ZN1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000006">
        <property type="entry name" value="Arginyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="entry name" value="Arginyl tRNA synthetase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002446">
        <property type="entry name" value="PRK01611.3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF5">
        <property type="entry name" value="ARGININE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="Arginyl-tRNA synthetase (ArgRS), N-terminal 'additional' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151656" description="Arginine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <sequence length="625" mass="71387" checksum="38893F7E4602C19F" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZP5</accession>
    <name>Q97ZP5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Third ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO6568</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK67512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZP5"/>
    <dbReference type="STRING" id="273057.SSO6568"/>
    <dbReference type="PaxDb" id="273057-SSO6568"/>
    <dbReference type="EnsemblBacteria" id="AAK67512">
        <property type="protein sequence ID" value="AAK67512"/>
        <property type="gene ID" value="SSO6568"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO6568"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.812"/>
    <dbReference type="HOGENOM" id="CLU_2519931_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZP5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK67512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="84" mass="9764" checksum="95690FAB4D42A904" modified="2001-10-01" version="1">MSKDEIVSRGASGYYGIQDKANNSRRWLLLMCSNSFHVIAVPVGDVKVYEEMGITYSKRHRRDEAKFRLLVYTREEEENSCLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZP7</accession>
    <name>Q97ZP7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO6565</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK67511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZP7"/>
    <dbReference type="STRING" id="273057.SSO6565"/>
    <dbReference type="PaxDb" id="273057-SSO6565"/>
    <dbReference type="EnsemblBacteria" id="AAK67511">
        <property type="protein sequence ID" value="AAK67511"/>
        <property type="gene ID" value="SSO6565"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO6565"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.811"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208840_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZP7"/>
    <dbReference type="PhylomeDB" id="Q97ZP7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK67511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="61" mass="7431" checksum="60F3B82B589516FD" modified="2001-10-01" version="1">MFFACWCSCWCSTPCIFKEMMGREEFRLLLILLFSIDLFTENFPFKLIETLIYTIDLFSRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-09" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZT9</accession>
    <name>PSTB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate import ATP-binding protein PstB</fullName>
            <ecNumber evidence="1">7.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ABC phosphate transporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphate-transporting ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pstB</name>
        <name type="ordered locus">SSO0488</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate(out) + ATP + H2O = ADP + 2 phosphate(in) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Phosphate importer (TC 3.A.1.7) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90194">
        <property type="entry name" value="B90194"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923003.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZT9"/>
    <dbReference type="SMR" id="Q97ZT9"/>
    <dbReference type="FunCoup" id="Q97ZT9">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0488"/>
    <dbReference type="PaxDb" id="273057-SSO0488"/>
    <dbReference type="EnsemblBacteria" id="AAK40809">
        <property type="protein sequence ID" value="AAK40809"/>
        <property type="gene ID" value="SSO0488"/>
    </dbReference>
    <dbReference type="GeneID" id="7807109"/>
    <dbReference type="KEGG" id="sso:SSO0488"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.482"/>
    <dbReference type="eggNOG" id="arCOG00231">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="InParanoid" id="Q97ZT9"/>
    <dbReference type="PhylomeDB" id="Q97ZT9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015415">
        <property type="term" value="F:ATPase-coupled phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="PstB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010840">
        <property type="entry name" value="PRK14246.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423:SF1">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51238">
        <property type="entry name" value="PSTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0592">Phosphate transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000092953" description="Phosphate import ATP-binding protein PstB">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="11"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="43"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01702"/>
        </source>
    </evidence>
    <sequence length="257" mass="29010" checksum="3F019133AD953602" modified="2001-10-01" version="1">MEAGKSAEDVFNISRLYLYINDKAILKDITIKIPNNSIFGIMGPSGSGKSTLLKVLNRLIEIYDSKIKVDGKVLYFGKDIFQIDAIKLRKEVGMVFQQPNPFPHLSIYDNIAYPLKSHGIKEKREIKKIVEECLRKVGLWKEVYDRLNSPASQLSGGQQQRLTIARALALKPKVLLMDEPTSMIDIVNSQAIEKLITELKNEIAIVIVSHNPQQVARVADYVAFLYNGELVEWGSSNEIFTSPKNELTEKYVIGRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZX5</accession>
    <name>Q97ZX5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH bat-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0447</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90189">
        <property type="entry name" value="F90189"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988714.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZX5"/>
    <dbReference type="STRING" id="273057.SSO0447"/>
    <dbReference type="PaxDb" id="273057-SSO0447"/>
    <dbReference type="EnsemblBacteria" id="AAK40773">
        <property type="protein sequence ID" value="AAK40773"/>
        <property type="gene ID" value="SSO0447"/>
    </dbReference>
    <dbReference type="GeneID" id="1455583"/>
    <dbReference type="KEGG" id="sso:SSO0447"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.441"/>
    <dbReference type="eggNOG" id="arCOG02271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101251_2_0_2"/>
    <dbReference type="InParanoid" id="Q97ZX5"/>
    <dbReference type="PhylomeDB" id="Q97ZX5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH bat-type" evidence="1">
        <location>
            <begin position="134"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="194" mass="22266" checksum="FDC12DFD5D0D174A" modified="2001-10-01" version="1">MLLYVVIKTPNLLQFPEFIPKKVTVNILDIRVDGEKGKLLLEIRGDETSAKQVCEEPIKVSKYKFICTNYISSELLKILSQYTIMNGALTDDGIFWTLILNGYTELRELLNSLIDITKEVRVLKVVKAEKKDAITARQEQILRIALEAGFFDYPRRIGLKDLAKKLNISPSSLSEIIRRAEKNVITAYFEEREL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-20" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q980A8</accession>
    <name>RS15_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein uS15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">30S ribosomal protein S15</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps15</name>
        <name evidence="5" type="synonym">rps13e</name>
        <name type="ordered locus">SSO0408</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90185">
        <property type="entry name" value="B90185"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988775.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="Q=1-152"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="Q=1-152"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="Q=1-152"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="Q=1-152"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="Q=1-152"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="Q=1-152"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="Q=1-152"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="Q=1-152"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q980A8"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q980A8"/>
    <dbReference type="FunCoup" id="Q980A8">
        <property type="interactions" value="222"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0408"/>
    <dbReference type="PaxDb" id="273057-SSO0408"/>
    <dbReference type="EnsemblBacteria" id="AAK40737">
        <property type="protein sequence ID" value="AAK40737"/>
        <property type="gene ID" value="SSO0408"/>
    </dbReference>
    <dbReference type="GeneID" id="1455547"/>
    <dbReference type="KEGG" id="sso:SSO0408"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.404"/>
    <dbReference type="eggNOG" id="arCOG04185">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090139_2_0_2"/>
    <dbReference type="InParanoid" id="Q980A8"/>
    <dbReference type="PhylomeDB" id="Q980A8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00677">
        <property type="entry name" value="S15_NS1_EPRS_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000003">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.130:FF:000001">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_A">
        <property type="entry name" value="Ribosomal_uS15_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_uS15_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012606">
        <property type="entry name" value="Ribosomal_uS15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006331">
        <property type="entry name" value="PRK08561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15P/S13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885:SF6">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000115621" description="Small ribosomal subunit protein uS15">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="152" mass="17441" checksum="DF9D45EB8C2D6FAC" modified="2001-10-01" version="1">MNKRRAKGKSHSIRPARAGAPKWVRLTREEVEMLVEELAKRGYTPSMIGIILRDQYGIPLVKQIVGKKVTQILEERGLAPQIPEDLFNLIRKAVNVRRHINEYPRDKTAKKGLEEIESKIRRLTRYYKGIGKLPQEWVYDPAKAELLVAGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q980E1</accession>
    <name>Q980E1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ferripyochelin binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0372</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90180">
        <property type="entry name" value="G90180"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990675.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980E1"/>
    <dbReference type="FunCoup" id="Q980E1">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0372"/>
    <dbReference type="PaxDb" id="273057-SSO0372"/>
    <dbReference type="EnsemblBacteria" id="AAK40702">
        <property type="protein sequence ID" value="AAK40702"/>
        <property type="gene ID" value="SSO0372"/>
    </dbReference>
    <dbReference type="GeneID" id="1455512"/>
    <dbReference type="KEGG" id="sso:SSO0372"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.366"/>
    <dbReference type="eggNOG" id="arCOG01849">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064827_4_1_2"/>
    <dbReference type="InParanoid" id="Q980E1"/>
    <dbReference type="PhylomeDB" id="Q980E1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04650">
        <property type="entry name" value="LbH_FBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050484">
        <property type="entry name" value="Transf_Hexapept/Carb_Anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13061">
        <property type="entry name" value="DYNACTIN SUBUNIT P25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13061:SF29">
        <property type="entry name" value="GAMMA CARBONIC ANHYDRASE-LIKE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21711">
        <property type="entry name" value="DCTN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="169" mass="18516" checksum="DC8962F955029DDD" modified="2001-10-01" version="1">MPIEEYLGKTPKVSQKAYIHPTSYIIGDVEIGDLTSIWHYVVIRGDNDSIRIGKESNVQENTTIHTDYGYPVEIGDKVTIGHNAVIHGAKVSSHVIVGMGAILLNGSQVKEYSIIGAGSVVTQGTVIPPYSVAVGVPAKVIKKLREDEILIIDENAEEYLKHTRRLLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q980H5</accession>
    <name>Q980H5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH dehydrogenase subunit A (NuoA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NuoA</name>
        <name evidence="8" type="ordered locus">SSO0322</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90175">
        <property type="entry name" value="B90175"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990615.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980H5"/>
    <dbReference type="FunCoup" id="Q980H5">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0322"/>
    <dbReference type="PaxDb" id="273057-SSO0322"/>
    <dbReference type="EnsemblBacteria" id="AAK40657">
        <property type="protein sequence ID" value="AAK40657"/>
        <property type="gene ID" value="SSO0322"/>
    </dbReference>
    <dbReference type="GeneID" id="1455467"/>
    <dbReference type="KEGG" id="sso:SSO0322"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.316"/>
    <dbReference type="eggNOG" id="arCOG01557">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147805_0_0_2"/>
    <dbReference type="InParanoid" id="Q980H5"/>
    <dbReference type="PhylomeDB" id="Q980H5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030964">
        <property type="term" value="C:NADH dehydrogenase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1610">
        <property type="entry name" value="NADH:ubiquinone/plastoquinone oxidoreductase, chain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000440">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038430">
        <property type="entry name" value="NDAH_ubi_oxred_su3_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004726">
        <property type="entry name" value="PRK06073.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004728">
        <property type="entry name" value="PRK06073.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11058">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11058:SF9">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00507">
        <property type="entry name" value="Oxidored_q4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK40657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="129" mass="14888" checksum="859C2CD3AF726C5B" modified="2001-10-01" version="1">MSFTQAILGFGIPIVVFLAAGYGLYKFLLLILPSKPNPLKVSRFEAGNIPTGSGRLWFPLQYYGYLLLYVTLEPIIVLLLPIAFSNYYSVPVAFRDLLLIIVVFIVLMYPVLYYAIRQINLLYQWEMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q980H9</accession>
    <name>Q980H9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sugar phosphate nucleotydyl transferase</fullName>
            <ecNumber evidence="4">2.7.7.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0317</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90174">
        <property type="entry name" value="F90174"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990610.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980H9"/>
    <dbReference type="FunCoup" id="Q980H9">
        <property type="interactions" value="281"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0317"/>
    <dbReference type="PaxDb" id="273057-SSO0317"/>
    <dbReference type="EnsemblBacteria" id="AAK40653">
        <property type="protein sequence ID" value="AAK40653"/>
        <property type="gene ID" value="SSO0317"/>
    </dbReference>
    <dbReference type="GeneID" id="1455463"/>
    <dbReference type="KEGG" id="sso:SSO0317"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.311"/>
    <dbReference type="eggNOG" id="arCOG00666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_0_2"/>
    <dbReference type="InParanoid" id="Q980H9"/>
    <dbReference type="PhylomeDB" id="Q980H9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="3">
        <location>
            <begin position="250"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="361" mass="40519" checksum="5E618B14BC05E4CB" modified="2001-10-01" version="1">MVSAIVLAGGYATRLRPLSLTKPKALFPILNKPILGYILESLINSDVSDIYLSLRVMADKIIDYLKDTSMLDKVKIEVESEPLGDAGPLKLISQKYNLDDDVLVIYGDIYSEINIKSLLDFYYKKSCDAVVVGTEVEDPRRYGVLYTENDVLVELIEKPKKPISNLINGGVYIFKKDLFKLVETPSSISKDFLPKLLRTKSISVYKYHGIWADIGVPDDYLRLNFEVLVQKYPKGYINSSAKVSEKSTLIPPYYIGSKNVIGEDAYITSNTILGNDVEIGKGTYISESILMNKVQVKEYTYISGSIIADKTKIGRWNHILDGSILGEEVITSDGVLINRRTIILPNKEVKEHVYDKGKIIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2026-01-28" version="137" xmlns="https://uniprot.org/uniprot">
    <accession>Q980I5</accession>
    <name>AROA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
            <shortName evidence="1">EPSP synthase</shortName>
            <shortName evidence="1">EPSPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroA</name>
        <name type="ordered locus">SSO0309</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:21257"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EPSP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90173">
        <property type="entry name" value="G90173"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990601.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980I5"/>
    <dbReference type="SMR" id="Q980I5"/>
    <dbReference type="FunCoup" id="Q980I5">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0309"/>
    <dbReference type="PaxDb" id="273057-SSO0309"/>
    <dbReference type="EnsemblBacteria" id="AAK40646">
        <property type="protein sequence ID" value="AAK40646"/>
        <property type="gene ID" value="SSO0309"/>
    </dbReference>
    <dbReference type="GeneID" id="1455456"/>
    <dbReference type="GeneID" id="44129282"/>
    <dbReference type="KEGG" id="sso:SSO0309"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.302"/>
    <dbReference type="eggNOG" id="arCOG04134">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024321_0_0_2"/>
    <dbReference type="InParanoid" id="Q980I5"/>
    <dbReference type="PhylomeDB" id="Q980I5"/>
    <dbReference type="UniPathway" id="UPA00053"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="entry name" value="Enolpyruvate transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090">
        <property type="entry name" value="AROM/DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090:SF5">
        <property type="entry name" value="PENTAFUNCTIONAL AROM POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000088337" description="3-phosphoshikimate 1-carboxyvinyltransferase">
        <location>
            <begin position="1"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
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        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
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        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
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        </ligand>
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        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00210"/>
        </source>
    </evidence>
    <sequence length="414" mass="45322" checksum="775E3355FE30363C" modified="2001-10-01" version="1">MIVKIYPSKISGIIKAPQSKSLAIRLIFLSLFTRVYLHNLVLSEDVIDAIKSVRALGVKVKNNSEFIPPEKLEIKERFIKLKGSATTLRMLIPILAAIGGEVTIDADESLRRRPLNRIVQALSNYGISFSSYSLPLTITGKLSSNEIKISGDESSQYISGLIYALHILNGGSIEILPPISSKSYILLTIDLFKRFGSDVKFYGSKIHVNPNNLVEFQGEVAGDYGLASFYALSALVSGGGITITNLWEPKEYFGDHSIVKIFSEMGASSEYKDGRWFVKAKDKYSPIKIDIDDAPDLAMTIAGLSAIAEGTSEIIGIERLRIKESDRIESIRKILGLYGVGSEVKYNSILIFGINKGMLNSPVTDCLNDHRVAMMSSALALVNGGVITSAECVGKSNPNYWQDLLSLNAKISIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q980J5</accession>
    <name>Q980J5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90172">
        <property type="entry name" value="E90172"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990588.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980J5"/>
    <dbReference type="STRING" id="273057.SSO0296"/>
    <dbReference type="PaxDb" id="273057-SSO0296"/>
    <dbReference type="EnsemblBacteria" id="AAK40636">
        <property type="protein sequence ID" value="AAK40636"/>
        <property type="gene ID" value="SSO0296"/>
    </dbReference>
    <dbReference type="GeneID" id="1455446"/>
    <dbReference type="KEGG" id="sso:SSO0296"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.292"/>
    <dbReference type="eggNOG" id="arCOG05903">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_898978_0_0_2"/>
    <dbReference type="InParanoid" id="Q980J5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="298" mass="34813" checksum="33B21CC08DD911BC" modified="2001-10-01" version="1">MDKKYIAAIFLPFIVIIVYAYIFKINIIYVLQEDPKFFLIFLLTYTAQNFVSSFKDSLLIKRRVDITLRARLLGNFVGLLVPGWAGQELVRSLVYNKYGVNLRQAFSYSILIGSIDVLAICLGYILLLPFFFNPLEFVFILVVLFNIAGWIGALSYFYLQHDKVNKIEQFIFKIVKMERIVTDYMEFKYYLKQGTTKDFVIYIMIDIFSFALYGGYFYYVTHNIFLSIFISFLYQVSILIPIPSAAIVGELALSIFLNPSYVFATRLNYIISNLLGSIFFKDMSFEELKKWSKEILKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q980N3</accession>
    <name>Q980N3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS</fullName>
            <shortName evidence="6">tRNA(Ile2)-agm2C synthetase</shortName>
            <ecNumber evidence="6">6.3.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">tRNA(Ile2) agmatidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tiaS</name>
        <name evidence="10" type="ordered locus">SSO0258</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">ATP-dependent agmatine transferase that catalyzes the formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) of tRNA(Ile2), converting the codon specificity from AUG to AUA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(34) in tRNA(Ile2) + agmatine + ATP + H2O = 2-agmatinylcytidine(34) in tRNA(Ile2) + AMP + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43608"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10626"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83545"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TiaS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.22" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90167">
        <property type="entry name" value="F90167"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990523.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980N3"/>
    <dbReference type="STRING" id="273057.SSO0258"/>
    <dbReference type="PaxDb" id="273057-SSO0258"/>
    <dbReference type="EnsemblBacteria" id="AAK40597">
        <property type="protein sequence ID" value="AAK40597"/>
        <property type="gene ID" value="SSO0258"/>
    </dbReference>
    <dbReference type="GeneID" id="1455407"/>
    <dbReference type="KEGG" id="sso:SSO0258"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.252"/>
    <dbReference type="eggNOG" id="arCOG01115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_675459_0_0_2"/>
    <dbReference type="InParanoid" id="Q980N3"/>
    <dbReference type="PhylomeDB" id="Q980N3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04482">
        <property type="entry name" value="RPA2_OBF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01892">
        <property type="entry name" value="tRNA_Ile2_agm2C_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053870">
        <property type="entry name" value="TiaS-like_TCKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013696">
        <property type="entry name" value="TiaS_FLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024913">
        <property type="entry name" value="tRNA_Ile2__agm2C_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055394">
        <property type="entry name" value="Zn_ribbon_TiaS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40705">
        <property type="entry name" value="TRNA(ILE2) 2-AGMATINYLCYTIDINE SYNTHETASE TIAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40705:SF1">
        <property type="entry name" value="TRNA(ILE2) 2-AGMATINYLCYTIDINE SYNTHETASE TIAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08489">
        <property type="entry name" value="TiaS_FLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22641">
        <property type="entry name" value="TiaS_TCKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23783">
        <property type="entry name" value="Zn_ribbon_TiaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TiaS-like TCKD" evidence="8">
        <location>
            <begin position="4"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="TiaS FLD" evidence="7">
        <location>
            <begin position="146"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="TiaS C-terminal zinc ribbon" evidence="9">
        <location>
            <begin position="352"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK40597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="443" mass="51224" checksum="CD27F3D8DFD64E40" modified="2001-10-01" version="1">MKYVIGIDDHDSYKFGCTTHFSVILTSYLYKNHSTILLDLPYLVRLNPNIPWKTRGNASIKLIVEFNGTKKELADIIFSYSIEYVKDVSLALEHGRKPGIAIMEYDKYITLFDKLYDFYIKGISDIIPIDYAKKFAEKNDIELRGDRGIIGSIAGLGMSGDYTYELITYRKKENWLKKRMINKDSVKRVDESTFPLTFANYDYINDTPLITPHGTDPILYGIRGASIQHLIKAMELIESNEDIDFFAIFKTNQSTDIHFQKIGNRFYQETKKVVQVKNVRILEGGDVIVETTDNDILFVYKETGELNSAAKLLKKGDEIVAYGAVKPSIAYGKIIELERFEILKLYDLELVNPKCPRCGGSTNSLGKNKGYRCKKCKYIINTTNKSTKNIMRNLSLGMYQTRSYRHLTKPIFLELENNKPSFYEERKFLEMYRSTLYKLDYHL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2021-09-29" modified="2025-06-18" version="138" xmlns="https://uniprot.org/uniprot">
    <accession>Q980R1</accession>
    <accession>Q980R0</accession>
    <name>RPO2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit Rpo2</fullName>
            <ecNumber evidence="6">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpo2</name>
        <name evidence="3" type="synonym">rpoB1</name>
        <name evidence="3" type="synonym">rpoB2</name>
        <name type="ordered locus">SSO0227/SSO3254</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="journal article" date="2008" name="Nature" volume="451" first="851" last="854">
            <title>The X-ray crystal structure of RNA polymerase from Archaea.</title>
            <authorList>
                <person name="Hirata A."/>
                <person name="Klein B.J."/>
                <person name="Murakami K.S."/>
            </authorList>
            <dbReference type="PubMed" id="18235446"/>
            <dbReference type="DOI" id="10.1038/nature06530"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.40 ANGSTROMS) OF 479-1124 OF THE RNA POLYMERASE COMPLEX IN COMPLEX WITH ZINC</scope>
        <scope>SEQUENCE REVISION</scope>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (Probable). This subunit is involved in DNA promoter recognition.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 Zn(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 13-subunit RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="frameshift">
            <sequence resource="EMBL-CDS" id="AAK40567" version="1"/>
        </conflict>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="frameshift">
            <sequence resource="EMBL-CDS" id="AAK40568" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40568.1"/>
        <property type="status" value="ALT_FRAME"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40567.1"/>
        <property type="status" value="ALT_FRAME"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99164">
        <property type="entry name" value="A99164"/>
    </dbReference>
    <dbReference type="PIR" id="H90163">
        <property type="entry name" value="H90163"/>
    </dbReference>
    <dbReference type="PDB" id="2PMZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="B/R=5-1124"/>
    </dbReference>
    <dbReference type="PDB" id="3HKZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="B/J=5-1119"/>
    </dbReference>
    <dbReference type="PDBsum" id="2PMZ"/>
    <dbReference type="PDBsum" id="3HKZ"/>
    <dbReference type="AlphaFoldDB" id="Q980R1"/>
    <dbReference type="SMR" id="Q980R1"/>
    <dbReference type="DIP" id="DIP-60640N"/>
    <dbReference type="FunCoup" id="Q980R1">
        <property type="interactions" value="212"/>
    </dbReference>
    <dbReference type="IntAct" id="Q980R1">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3254"/>
    <dbReference type="PaxDb" id="273057-SSO0227"/>
    <dbReference type="EnsemblBacteria" id="AAK40567">
        <property type="protein sequence ID" value="AAK40567"/>
        <property type="gene ID" value="SSO0227"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK40568">
        <property type="protein sequence ID" value="AAK40568"/>
        <property type="gene ID" value="SSO3254"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0227"/>
    <dbReference type="KEGG" id="sso:SSO3254"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.223"/>
    <dbReference type="eggNOG" id="arCOG01762">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_2_2_2"/>
    <dbReference type="InParanoid" id="Q980R1"/>
    <dbReference type="PhylomeDB" id="Q980R1"/>
    <dbReference type="BRENDA" id="2.7.7.6">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q980R1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019969">
        <property type="entry name" value="RNAP_Rpo2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006335">
        <property type="entry name" value="PRK08565.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03670">
        <property type="entry name" value="rpoB_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000453793" description="DNA-directed RNA polymerase subunit Rpo2">
        <location>
            <begin position="1"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2 9 10">
        <location>
            <position position="1061"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 9 10">
        <location>
            <position position="1064"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 9 10">
        <location>
            <position position="1079"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1082"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="28"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="turn" evidence="11">
        <location>
            <begin position="40"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="turn" evidence="11">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="57"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="73"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="82"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="turn" evidence="11">
        <location>
            <begin position="86"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="93"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="99"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="118"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="126"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="129"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="135"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="154"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="159"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="169"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="181"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="186"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="194"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
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                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90159">
        <property type="entry name" value="D90159"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990422.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980U2"/>
    <dbReference type="STRING" id="273057.SSO0185"/>
    <dbReference type="PaxDb" id="273057-SSO0185"/>
    <dbReference type="EnsemblBacteria" id="AAK40531">
        <property type="protein sequence ID" value="AAK40531"/>
        <property type="gene ID" value="SSO0185"/>
    </dbReference>
    <dbReference type="GeneID" id="1455341"/>
    <dbReference type="KEGG" id="sso:SSO0185"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.184"/>
    <dbReference type="eggNOG" id="arCOG01377">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_510589_0_0_2"/>
    <dbReference type="InParanoid" id="Q980U2"/>
    <dbReference type="PhylomeDB" id="Q980U2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF120">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="545" mass="62381" checksum="3543ED8851D8C18C" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q980V4</accession>
    <name>Q980V4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription elongation factor NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2025" name="Structure" volume="33" first="149" last="159.e6">
            <title>Archaeal NusA2 is the ancestor of ribosomal protein eS7 in eukaryotes.</title>
            <authorList>
                <person name="Phung D.K."/>
                <person name="Pilotto S."/>
                <person name="Matelska D."/>
                <person name="Blombach F."/>
                <person name="Pinotsis N."/>
                <person name="Hovan L."/>
                <person name="Gervasio F.L."/>
                <person name="Werner F."/>
            </authorList>
            <dbReference type="PubMed" id="39504966"/>
            <dbReference type="DOI" id="10.1016/j.str.2024.10.019"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.68 ANGSTROMS) IN COMPLEX WITH ZN(2+)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90157">
        <property type="entry name" value="G90157"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990397.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9F9U">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.68 A"/>
        <property type="chains" value="A=1-170"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980V4"/>
    <dbReference type="SMR" id="Q980V4"/>
    <dbReference type="STRING" id="273057.SSO0172"/>
    <dbReference type="PaxDb" id="273057-SSO0172"/>
    <dbReference type="EnsemblBacteria" id="AAK40518">
        <property type="protein sequence ID" value="AAK40518"/>
        <property type="gene ID" value="SSO0172"/>
    </dbReference>
    <dbReference type="GeneID" id="1453345"/>
    <dbReference type="KEGG" id="sso:SSO0172"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.168"/>
    <dbReference type="eggNOG" id="arCOG01761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132460_1_0_2"/>
    <dbReference type="InParanoid" id="Q980V4"/>
    <dbReference type="PhylomeDB" id="Q980V4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005013">
        <property type="entry name" value="PRK06418.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="3" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="9F9U"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19540" checksum="A55F5C4A00B33F6B" modified="2001-10-01" version="1">MKMKIPLDYVCVRSGLLCNRCQSLIDSGEVFEYEVEIIKILLDLEETQFKELKDSTYHKAYKVDDLLILLVTSGQEMTQQKWIKIARILQEKLNIKVRVLEKTNSIKNSAVQLLSPARVLGVNTVWMPDGSVQYVIRVSRSERRLLPAEAQLLESALTKIHSTPVRIRVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-06-07" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q980V5</accession>
    <name>RL40_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL40</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L40e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl40e</name>
        <name type="ordered locus">SSO5336</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL40 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90157">
        <property type="entry name" value="F90157"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990396.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2AYJ">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-56"/>
    </dbReference>
    <dbReference type="PDBsum" id="2AYJ"/>
    <dbReference type="AlphaFoldDB" id="Q980V5"/>
    <dbReference type="SMR" id="Q980V5"/>
    <dbReference type="FunCoup" id="Q980V5">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO5336"/>
    <dbReference type="PaxDb" id="273057-SSO5336"/>
    <dbReference type="DNASU" id="1453344"/>
    <dbReference type="EnsemblBacteria" id="AAK40517">
        <property type="protein sequence ID" value="AAK40517"/>
        <property type="gene ID" value="SSO5336"/>
    </dbReference>
    <dbReference type="GeneID" id="42362137"/>
    <dbReference type="KEGG" id="sso:SSO5336"/>
    <dbReference type="eggNOG" id="arCOG04049">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175093_1_0_2"/>
    <dbReference type="InParanoid" id="Q980V5"/>
    <dbReference type="PhylomeDB" id="Q980V5"/>
    <dbReference type="EvolutionaryTrace" id="Q980V5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00788">
        <property type="entry name" value="Ribosomal_eL40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023657">
        <property type="entry name" value="Ribosomal_eL40_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001975">
        <property type="entry name" value="Ribosomal_eL40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="Ribosomal_eL40_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003161">
        <property type="entry name" value="PRK04136.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39649">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L40E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39649:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01020">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01377">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000138790" description="Large ribosomal subunit protein eL40">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="11"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="20"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="23"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="37"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="44"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="52"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2AYJ"/>
        </source>
    </evidence>
    <sequence length="56" mass="6445" checksum="61DE2F4A3EC13A65" modified="2001-10-01" version="1">MPLTDPAKLQIVQQRVFLKKVCRKCGALNPIRATKCRRCHSTNLRLKKKELPTKKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q980W4</accession>
    <name>UPPS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)</fullName>
            <ecNumber evidence="1">2.5.1.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl diphosphate synthase</fullName>
            <shortName evidence="1">UDS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl pyrophosphate synthase</fullName>
            <shortName evidence="1">UPP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uppS</name>
        <name type="ordered locus">SSO0163</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="29537" last="29549">
            <title>The Sulfolobus solfataricus Lrp-like protein LysM regulates lysine biosynthesis in response to lysine availability.</title>
            <authorList>
                <person name="Brinkman A.B."/>
                <person name="Bell S.D."/>
                <person name="Lebbink R.J."/>
                <person name="de Vos W.M."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="12042311"/>
            <dbReference type="DOI" id="10.1074/jbc.M203528200"/>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with geranylgeranyl diphosphate (GGPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-undecaprenyl diphosphate (tritrans,heptacis-UPP). It is probably the precursor of glycosyl carrier lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>geranylgeranyl diphosphate + 7 isopentenyl diphosphate = tri-trans,hepta-cis-undecaprenyl diphosphate + 7 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27622"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:60388"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="2.5.1.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.89" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BK000545">
        <property type="protein sequence ID" value="DAA00056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90156">
        <property type="entry name" value="E90156"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990387.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980W4"/>
    <dbReference type="SMR" id="Q980W4"/>
    <dbReference type="FunCoup" id="Q980W4">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0163"/>
    <dbReference type="PaxDb" id="273057-SSO0163"/>
    <dbReference type="EnsemblBacteria" id="AAK40508">
        <property type="protein sequence ID" value="AAK40508"/>
        <property type="gene ID" value="SSO0163"/>
    </dbReference>
    <dbReference type="GeneID" id="1453335"/>
    <dbReference type="GeneID" id="44129126"/>
    <dbReference type="KEGG" id="sso:SSO0163"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.159"/>
    <dbReference type="eggNOG" id="arCOG01532">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_0_4_2"/>
    <dbReference type="InParanoid" id="Q980W4"/>
    <dbReference type="PhylomeDB" id="Q980W4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000123742" description="Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="41"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="85"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="217"/>
            <end position="219"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <sequence length="262" mass="31017" checksum="723828C346D1CC09" modified="2001-10-01" version="1">MAKDLTRGLLFKPIYKLYELVLWNQIRNGPLPDHVGIIPDGNRRWARDNNLSLNDAYFYGYKKLKEVLIWLLELGIKNITVFVLSTENCDKRKQNELSIITTYIKRGIQDLLEDPIVDKYEVKVSAIGKLDKLPKDLIDYLNRVVEKTSNYDKRKLTLAICYGGRQEILDAVVRLLDDYRNGKIVKSEINEETFRKYFYDSELQDIDLVIRTSGEVRISNFLLWHVAYSELFFCEAYWPEFRKIDLWRAIRSFQKRKRNFGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q980W9</accession>
    <name>LYSM_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>HTH-type transcriptional regulator LysM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">lysM</name>
        <name type="ordered locus">SSO0157</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="29537" last="29549">
            <title>The Sulfolobus solfataricus Lrp-like protein LysM regulates lysine biosynthesis in response to lysine availability.</title>
            <authorList>
                <person name="Brinkman A.B."/>
                <person name="Bell S.D."/>
                <person name="Lebbink R.J."/>
                <person name="de Vos W.M."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="12042311"/>
            <dbReference type="DOI" id="10.1074/jbc.M203528200"/>
        </citation>
        <scope>FUNCTION IN LYSINE BIOSYNTHESIS</scope>
        <scope>INDUCTION</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">In the absence or at low concentrations of lysine, activates the biosynthesis of this amino acid via the alpha-aminoadipate (AAA) pathway.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway [regulation].</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="2">Constitutively expressed.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BK000545">
        <property type="protein sequence ID" value="DAA00051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90155">
        <property type="entry name" value="H90155"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990382.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980W9"/>
    <dbReference type="SMR" id="Q980W9"/>
    <dbReference type="STRING" id="273057.SSO0157"/>
    <dbReference type="PaxDb" id="273057-SSO0157"/>
    <dbReference type="EnsemblBacteria" id="AAK40503">
        <property type="protein sequence ID" value="AAK40503"/>
        <property type="gene ID" value="SSO0157"/>
    </dbReference>
    <dbReference type="GeneID" id="1453330"/>
    <dbReference type="GeneID" id="44129119"/>
    <dbReference type="KEGG" id="sso:SSO0157"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.154"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_3_2"/>
    <dbReference type="InParanoid" id="Q980W9"/>
    <dbReference type="PhylomeDB" id="Q980W9"/>
    <dbReference type="UniPathway" id="UPA00033"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053483">
        <property type="entry name" value="HTH-Lysine_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040947">
        <property type="entry name" value="trans_reg_LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF4">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0010">Activator</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000111742" description="HTH-type transcriptional regulator LysM">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="HTH asnC-type" evidence="1">
        <location>
            <begin position="6"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12042311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="142" mass="16096" checksum="6AEBF0220E6A727A" modified="2001-10-01" version="1">MGNANIDESDLKILEILKKNARTPYTLIAKELKVSEAAIRKRIEKLIRQGIIKRFTIEYELENEIRAIVMVQSTPQIPTPEISKKIAKIPGVEVVYETTGDYDILVIVRGTNITSINRTIDEIRSIQGVVGTNSTIILRTWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q981D2</accession>
    <name>Q981D2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90140">
        <property type="entry name" value="E90140"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989636.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981D2"/>
    <dbReference type="PaxDb" id="273057-SSO0013"/>
    <dbReference type="EnsemblBacteria" id="AAK40380">
        <property type="protein sequence ID" value="AAK40380"/>
        <property type="gene ID" value="SSO0013"/>
    </dbReference>
    <dbReference type="GeneID" id="1455271"/>
    <dbReference type="KEGG" id="sso:SSO0013"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.14"/>
    <dbReference type="eggNOG" id="arCOG05910">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1324071_0_0_2"/>
    <dbReference type="InParanoid" id="Q981D2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="207" mass="24188" checksum="E333F826720F5094" modified="2001-10-01" version="1">MLLYFKPTKKERDINVALEIVSELFASRIGKLMGLPILEVSLIKHGDEIGLLMDYLPDKATEQNIYNLDEIKMSLAFEEWILNIDLKEDHVLSKNGKGFIIDHGHSLSAWKPLYYIIQIIDKKVSRFNLWASEDDFRDGIELLSSIDHNTISEILKESFSEVVESNFCKLFTREVAAEYMDLNIKILEKRMKYLKDGKILFTYNYRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q981D9</accession>
    <name>Q981D9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase, probably IS605-TnpB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90139">
        <property type="entry name" value="F90139"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989645.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981D9"/>
    <dbReference type="STRING" id="273057.SSO0008"/>
    <dbReference type="PaxDb" id="273057-SSO0008"/>
    <dbReference type="EnsemblBacteria" id="AAK40373">
        <property type="protein sequence ID" value="AAK40373"/>
        <property type="gene ID" value="SSO0008"/>
    </dbReference>
    <dbReference type="GeneID" id="1455263"/>
    <dbReference type="KEGG" id="sso:SSO0008"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.8"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1017881_0_0_2"/>
    <dbReference type="InParanoid" id="Q981D9"/>
    <dbReference type="PhylomeDB" id="Q981D9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="212"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="282" mass="32450" checksum="677807E86D333DFB" modified="2001-10-01" version="1">MKESLIEAKVMDYGKLKDIQKEILYYKLYVDALKKKGIKEKVDPPPTLPKSIVSSVLTGGIPRDGPLQIELVSNDVVKIKEYNTLVKIQSDSRLPLYAIVEYRDTDIRVYLAYRENPSIVGLDLGLRHLITIVAIKDTKPWKVRFFDEPKLMEKFANFLSENQGIIKLEEMKSNAKRIIYEAVSFIEDLEPKVVAMENLEYFETKTGKGLRALQNMLENEIRRRGIRYRKIDPYNTSRVCAKCGYKKGEILGSLFVCPACGYKADRDYNAAYNIALKCYYTC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-01-24" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UWW9</accession>
    <name>AATD_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">A-type ATP synthase subunit D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpD</name>
        <name type="ordered locus">SSO0566</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90203">
        <property type="entry name" value="B90203"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991078.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UWW9"/>
    <dbReference type="SMR" id="Q9UWW9"/>
    <dbReference type="FunCoup" id="Q9UWW9">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0566"/>
    <dbReference type="PaxDb" id="273057-SSO0566"/>
    <dbReference type="EnsemblBacteria" id="AAK40881">
        <property type="protein sequence ID" value="AAK40881"/>
        <property type="gene ID" value="SSO0566"/>
    </dbReference>
    <dbReference type="GeneID" id="1454848"/>
    <dbReference type="KEGG" id="sso:SSO0566"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.574"/>
    <dbReference type="eggNOG" id="arCOG04101">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069688_2_2_2"/>
    <dbReference type="InParanoid" id="Q9UWW9"/>
    <dbReference type="PhylomeDB" id="Q9UWW9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033176">
        <property type="term" value="C:proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3240:FF:000012">
        <property type="entry name" value="V-type ATP synthase subunit D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00271">
        <property type="entry name" value="ATP_synth_D_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001544">
        <property type="entry name" value="PRK00373.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00309">
        <property type="entry name" value="V_ATPase_subD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000144259" description="A-type ATP synthase subunit D">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00271"/>
        </source>
    </evidence>
    <sequence length="213" mass="25092" checksum="8943933520729DE8" modified="2000-05-01" version="1">MSQKVLPTKINLIQFRRQLRLITVIKRLLENKREVLLLYLRTYASQYEKIYNEVNEEMKKVYESYLQAVASEGISNIEEMALSQKPSLEVNSAIKVIFGVKVPTIRLDKSTIPAKPFSDVETSPYLSESYEEMTEALNKIIELVELESTIRSLVSELRKTQRLINSIDNYILPFYRGSIKFIKQILEDRQREEFSRLKIIRRILQRRRESGSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX79</accession>
    <name>ARCH_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein archease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0690</name>
        <name type="ORF">C10_040</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Activates the tRNA-splicing ligase complex by facilitating the enzymatic turnover of catalytic subunit RtcB. Acts by promoting the guanylylation of RtcB, a key intermediate step in tRNA ligation. Can also alter the NTP specificity of RtcB such that ATP, dGTP or ITP is used efficiently.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archease family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90216">
        <property type="entry name" value="G90216"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991237.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX79"/>
    <dbReference type="SMR" id="Q9UX79"/>
    <dbReference type="FunCoup" id="Q9UX79">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0690"/>
    <dbReference type="PaxDb" id="273057-SSO0690"/>
    <dbReference type="EnsemblBacteria" id="AAK40990">
        <property type="protein sequence ID" value="AAK40990"/>
        <property type="gene ID" value="SSO0690"/>
    </dbReference>
    <dbReference type="GeneID" id="1454957"/>
    <dbReference type="KEGG" id="sso:SSO0690"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.689"/>
    <dbReference type="eggNOG" id="arCOG04055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111362_3_0_2"/>
    <dbReference type="InParanoid" id="Q9UX79"/>
    <dbReference type="PhylomeDB" id="Q9UX79"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.10.10">
        <property type="entry name" value="Archease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01222">
        <property type="entry name" value="Archease_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002804">
        <property type="entry name" value="Archease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022952">
        <property type="entry name" value="Archease_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023572">
        <property type="entry name" value="Archease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036820">
        <property type="entry name" value="Archease_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001617">
        <property type="entry name" value="PRK00407.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682">
        <property type="entry name" value="ARCHEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682:SF11">
        <property type="entry name" value="PROTEIN ARCHEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01951">
        <property type="entry name" value="Archease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69819">
        <property type="entry name" value="MTH1598-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0106">Calcium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000068853" description="Protein archease">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01222"/>
        </source>
    </evidence>
    <sequence length="139" mass="16454" checksum="C5E883DBDC746730" modified="2000-05-01" version="1">MRQFEFFEHTADVGIKSYGRSLEEAFSNAALGVFEVITDTSKVRPVEYREIYLNGYDLENLLYKWIEELLYYYDSELMIFSKFDLMIDQDSITLEGKAWGERFNDKIHERRTVVKAMTYHQLSIEKTESGYVITFVVDI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2025-06-18" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXG7</accession>
    <name>KTHY1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Probable thymidylate kinase 1</fullName>
            <ecNumber>2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>dTMP kinase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">tmk1</name>
        <name type="synonym">tmk</name>
        <name type="ordered locus">SSO0780</name>
        <name type="ORF">C40_016</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>dTMP + ATP = dTDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90227">
        <property type="entry name" value="F90227"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923072.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXG7"/>
    <dbReference type="SMR" id="Q9UXG7"/>
    <dbReference type="FunCoup" id="Q9UXG7">
        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0780"/>
    <dbReference type="PaxDb" id="273057-SSO0780"/>
    <dbReference type="EnsemblBacteria" id="AAK41077">
        <property type="protein sequence ID" value="AAK41077"/>
        <property type="gene ID" value="SSO0780"/>
    </dbReference>
    <dbReference type="GeneID" id="7806740"/>
    <dbReference type="GeneID" id="7811867"/>
    <dbReference type="KEGG" id="sso:SSO0780"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.781"/>
    <dbReference type="eggNOG" id="arCOG01891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049131_1_3_2"/>
    <dbReference type="InParanoid" id="Q9UXG7"/>
    <dbReference type="PhylomeDB" id="Q9UXG7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01672">
        <property type="entry name" value="TMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF4">
        <property type="entry name" value="UMP-CMP KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155398" description="Probable thymidylate kinase 1">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="189" mass="21889" checksum="69BA0E750AAE0444" modified="2000-05-01" version="1">MQKLIAIEGIDGSGKTTLANLLKEHLESKMKLNVIVTREPFSEDIIKLIEKIGWNDPILLVLLFAADREIHVNWLSKIKDADLIILDRYYFSSIAYQGALGVDEQWIKMVNSYFPKPDMVILLDLPIEVAISRIKNDKFNFEEKIKSLAKVREKYLKLAKEYNFYVVDASKDKNEVLEQAIKIIQKNLF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-10-18" modified="2025-04-09" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>O93774</accession>
    <name>LRS14_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>HTH-type transcriptional regulator lrs14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">lrs14</name>
        <name type="ordered locus">SSO1108</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1999" name="J. Bacteriol." volume="181" first="1474" last="1480">
            <title>An Lrp-like protein of the hyperthermophilic archaeon Sulfolobus solfataricus which binds to its own promoter.</title>
            <authorList>
                <person name="Napoli A."/>
                <person name="van der Oost J."/>
                <person name="Sensen C.W."/>
                <person name="Charlebois R.L."/>
                <person name="Rossi M."/>
                <person name="Ciaramella M."/>
            </authorList>
            <dbReference type="PubMed" id="10049378"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>CHARACTERIZATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Acts in transcription regulation. Binds to multiple sequences in its own promoter.</text>
    </comment>
    <comment type="subunit">
        <text>Homodimer.</text>
    </comment>
    <dbReference type="EMBL" id="AF098294">
        <property type="protein sequence ID" value="AAD19358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90263">
        <property type="entry name" value="F90263"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923198.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O93774"/>
    <dbReference type="SMR" id="O93774"/>
    <dbReference type="STRING" id="273057.SSO1108"/>
    <dbReference type="PaxDb" id="273057-SSO1108"/>
    <dbReference type="EnsemblBacteria" id="AAK41365">
        <property type="protein sequence ID" value="AAK41365"/>
        <property type="gene ID" value="SSO1108"/>
    </dbReference>
    <dbReference type="GeneID" id="1454144"/>
    <dbReference type="GeneID" id="27427421"/>
    <dbReference type="KEGG" id="sso:SSO1108"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1105"/>
    <dbReference type="eggNOG" id="arCOG02242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150444_0_0_2"/>
    <dbReference type="InParanoid" id="O93774"/>
    <dbReference type="PhylomeDB" id="O93774"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053795">
        <property type="entry name" value="Lrs14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040939">
        <property type="entry name" value="trans_reg_lrs14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0010">Activator</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000111741" description="HTH-type transcriptional regulator lrs14">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="125" mass="14071" checksum="196986B515535D54" modified="1999-05-01" version="1">MQVENIRVRLPSGKEVGLVDALNFCYDISDTDFQVLKTLLNSGPKTEDELAEMLHLSKASINRSVNKLVSLGFVDRVKDSSSKGGRPRYIYKPIDADRITTKISNDFKYCADLFSSVIPQELKEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>P95862</accession>
    <name>DLHH_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Putative carboxymethylenebutenolidase</fullName>
            <ecNumber>3.1.1.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Dienelactone hydrolase</fullName>
            <shortName>DLH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2087</name>
        <name type="ORF">C06015</name>
        <name type="ORF">C31_045</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-2-enedioate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12372"/>
            <dbReference type="ChEBI" id="CHEBI:12040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57263"/>
            <dbReference type="EC" id="3.1.1.45"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dienelactone hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.45"/>
    <dbReference type="EMBL" id="Y08256">
        <property type="protein sequence ID" value="CAA69498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73082">
        <property type="entry name" value="S73082"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989796.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95862"/>
    <dbReference type="SMR" id="P95862"/>
    <dbReference type="FunCoup" id="P95862">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2087"/>
    <dbReference type="ESTHER" id="sulso-dlhh">
        <property type="family name" value="Dienelactone_hydrolase"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2087"/>
    <dbReference type="EnsemblBacteria" id="AAK42266">
        <property type="protein sequence ID" value="AAK42266"/>
        <property type="gene ID" value="SSO2087"/>
    </dbReference>
    <dbReference type="GeneID" id="1453593"/>
    <dbReference type="KEGG" id="sso:SSO2087"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2165"/>
    <dbReference type="eggNOG" id="arCOG01659">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054590_7_2_2"/>
    <dbReference type="InParanoid" id="P95862"/>
    <dbReference type="PhylomeDB" id="P95862"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008806">
        <property type="term" value="F:carboxymethylenebutenolidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051049">
        <property type="entry name" value="Dienelactone_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46623:SF6">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46623">
        <property type="entry name" value="CARBOXYMETHYLENEBUTENOLIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000161584" description="Putative carboxymethylenebutenolidase">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="257" mass="29016" checksum="B2BD7FACD6126382" modified="1997-05-01" version="1">MEKEIFYDSDGAKIRAFLATPENPKLAVIVIHEIWGLNDNIKDISRRLANEGYMALAPQLYTRNEDVLNEGNIQNVMMKVWSIPPEKRNDPNSYQQIMSALDEKGKKVAELLVLNRQKTEEQMIKDAIKAYEYVSSQGVKKIVSMGFCMGGGLAFQLATEVPLDGTIVFYGRNPQPLEAIQKIKGPILGLYAGEDPPIDAGLPDLISAIIKYKKDLELKIYPGAYHAFFNDRGRSYNKEAAEDAWERVKSFLRRVSK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>P95997</accession>
    <name>IDI2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isopentenyl-diphosphate delta-isomerase</fullName>
            <shortName evidence="1">IPP isomerase</shortName>
            <ecNumber evidence="1">5.3.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Isopentenyl diphosphate:dimethylallyl diphosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopentenyl pyrophosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Type 2 isopentenyl diphosphate isomerase</fullName>
            <shortName evidence="1">IDI-2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fni</name>
        <name type="ordered locus">SSO0063</name>
        <name type="ORF">C05008</name>
        <name type="ORF">C05_047</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homooctamer. Dimer of tetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IPP isomerase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="1"/>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75425">
        <property type="entry name" value="S75425"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988866.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95997"/>
    <dbReference type="SMR" id="P95997"/>
    <dbReference type="FunCoup" id="P95997">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0063"/>
    <dbReference type="PaxDb" id="273057-SSO0063"/>
    <dbReference type="EnsemblBacteria" id="AAK40424">
        <property type="protein sequence ID" value="AAK40424"/>
        <property type="gene ID" value="SSO0063"/>
    </dbReference>
    <dbReference type="GeneID" id="1455315"/>
    <dbReference type="GeneID" id="44129025"/>
    <dbReference type="KEGG" id="sso:SSO0063"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.63"/>
    <dbReference type="eggNOG" id="arCOG00613">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065515_1_0_2"/>
    <dbReference type="InParanoid" id="P95997"/>
    <dbReference type="PhylomeDB" id="P95997"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02811">
        <property type="entry name" value="IDI-2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00354">
        <property type="entry name" value="Idi_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011179">
        <property type="entry name" value="IPdP_isomerase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02151">
        <property type="entry name" value="IPP_isom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43665">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43665:SF1">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003314">
        <property type="entry name" value="IPP_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000134455" description="Isopentenyl-diphosphate delta-isomerase">
        <location>
            <begin position="1"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="8"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="66"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="96"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="275"/>
            <end position="277"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="296"/>
            <end position="297"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00354"/>
        </source>
    </evidence>
    <sequence length="368" mass="40536" checksum="18E6687879E83826" modified="1997-05-01" version="1">MPDIVNRKVEHVEIAAFENVDGLSSSTFLNDVILVHQGFPGISFSEINTKTKFFRKEISAPIMVTGMTGGRNELGRINRIIAEVAEKFGIPMGVGSQRVAIEKAEARESFTIVRKVAPTIPIIANLGMPQLVKGYGLKEFQDAIQMIEADAIAVHLNPAQEVFQPEGEPEYQIYALERLRDISKELSVPIIVKESGNGISMETAKLLYSYGIKNFDTSGQGGTNWIAIEMIRDIRRGNWKAESAKNFLDWGVPTAASIIEVRYSIPDAFLVGSGGIRSGLDAAKAIALGADIAGMALPVLKSAIEGKESLEQFFRKIIFELKATMMLTGSKNVEALKRSSIVILGKLKEWAEYRGINLSIYEKVRKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXK9</accession>
    <name>Q7LXK9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Sugar phosphate nucleotydyl transferase</fullName>
            <ecNumber evidence="13">2.7.7.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">SSO0745</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate + UTP + H(+) = UDP-N-acetyl-alpha-D-glucosamine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>alpha-D-glucosamine 1-phosphate + acetyl-CoA = N-acetyl-alpha-D-glucosamine 1-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="13"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99223">
        <property type="entry name" value="A99223"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991321.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXK9"/>
    <dbReference type="SMR" id="Q7LXK9"/>
    <dbReference type="FunCoup" id="Q7LXK9">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0745"/>
    <dbReference type="PaxDb" id="273057-SSO0745"/>
    <dbReference type="EnsemblBacteria" id="AAK41040">
        <property type="protein sequence ID" value="AAK41040"/>
        <property type="gene ID" value="SSO0745"/>
    </dbReference>
    <dbReference type="GeneID" id="1455007"/>
    <dbReference type="KEGG" id="sso:SSO0745"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.740"/>
    <dbReference type="eggNOG" id="arCOG00666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_1_2"/>
    <dbReference type="InParanoid" id="Q7LXK9"/>
    <dbReference type="PhylomeDB" id="Q7LXK9"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05636">
        <property type="entry name" value="LbH_G1P_TT_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023915">
        <property type="entry name" value="Bifunctiontional_GlmU_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050065">
        <property type="entry name" value="GlmU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053650">
        <property type="entry name" value="Sugar-1P_NT/AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03992">
        <property type="entry name" value="Arch_glmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041173">
        <property type="entry name" value="Sug_nt_acttase_Thmprot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584:SF8">
        <property type="entry name" value="N-ACETYLMURAMATE ALPHA-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584">
        <property type="entry name" value="NUCLEOTIDYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="11">
        <location>
            <begin position="2"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="12">
        <location>
            <begin position="256"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAK41040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="407" mass="45145" checksum="C32BD5CD7232766E" modified="2011-05-31" version="1">MKAVVLAAGKGERLEPITHTRPKPFVPVLETPLILRHIRILKKYINKIIIVINSNHKDYFKTIEGVSLVEQTEGKGTAAALRAAEKYLEGDEEFLVIYGDLLFEEDALDKIVNTEGEAILARESEDPRKFGVIVKDSENRLVRIVEKPENPPSNIINAGIYKFTYDIFSYIDKISLSSRGEFELTDAVNLIGNKVKVVTYNGIWLDIGRPWDLIEANKVLLDKEKDRNLGVIEENVKIKGKVVIEDGVIIKSGTYIEGPVYIGKNSVIGPNAYIRPYSVIGSNVKVGAFNEIKESVIMENAKIPHLSYVGDSVICEGVNFGAGTITANLRFDEEEVKVNIKNERVSSGRKKLGAIVGAHVRTGINVSILPGVKIGAYAWIYPGAVVDRDVEKGEKYVPYYLRRSSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXM4</accession>
    <name>Q7LXM4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter, ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0688</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90216">
        <property type="entry name" value="E90216"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXM4"/>
    <dbReference type="FunCoup" id="Q7LXM4">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0688"/>
    <dbReference type="PaxDb" id="273057-SSO0688"/>
    <dbReference type="EnsemblBacteria" id="AAK40988">
        <property type="protein sequence ID" value="AAK40988"/>
        <property type="gene ID" value="SSO0688"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0688"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.687"/>
    <dbReference type="eggNOG" id="arCOG00922">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="InParanoid" id="Q7LXM4"/>
    <dbReference type="PhylomeDB" id="Q7LXM4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098796">
        <property type="term" value="C:membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000032">
        <property type="entry name" value="Export ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42798:SF7">
        <property type="entry name" value="ALPHA-D-RIBOSE 1-METHYLPHOSPHONATE 5-TRIPHOSPHATE SYNTHASE SUBUNIT PHNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42798">
        <property type="entry name" value="LIPOPROTEIN-RELEASING SYSTEM ATP-BINDING PROTEIN LOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="23"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="246" mass="27600" checksum="FD4C8B2F239E1AFE" modified="2011-05-31" version="1">MPKDFLEGANRVDYMTDILIDAMDLKKVYKTKNIEYVALRGVTLKVNKGEFLVIAGPSGSGKTTLLDLLGLLDSPSAGKIIIDGIDVTNFDEDERAIFRRRYIGFVFQSYNLITYLTVLENVELALAAIGIPVWKRREKAEEILSMIPGMLELKNKKPNELSGGQQQRVAIARALANDPKILLADEPTANLDSKTGEAIVELMKKLNEEKKVTIIMATHDPDMMRYADRIVYIRDGLVEKEVIQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXM9</accession>
    <name>Q7LXM9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90215">
        <property type="entry name" value="H90215"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991229.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXM9"/>
    <dbReference type="FunCoup" id="Q7LXM9">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0681"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO0681"/>
    <dbReference type="EnsemblBacteria" id="AAK40983">
        <property type="protein sequence ID" value="AAK40983"/>
        <property type="gene ID" value="SSO0681"/>
    </dbReference>
    <dbReference type="GeneID" id="1454950"/>
    <dbReference type="KEGG" id="sso:SSO0681"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.682"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017896_3_0_2"/>
    <dbReference type="InParanoid" id="Q7LXM9"/>
    <dbReference type="PhylomeDB" id="Q7LXM9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004377">
        <property type="term" value="F:GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038013">
        <property type="entry name" value="ALG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919:SF1">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="181"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="355" mass="40388" checksum="8E735F3EFD820210" modified="2011-05-31" version="1">MKKIKVAVIAHGLGMSMGYSGEGNIYKTFFEMLEERKIDYTAISFAKPYNTSIPSVYTLPFHLPKLDKYQRLLTYYTAKKVKPDLYLNASGVPIPLSKIAPHIVYAGAPSIANLPSKYTRSLFWKLYLLPFRLVINKIKEEGKRAKIIANSRYSAKAIAEVYEISEPKVIYPPVDVGYFQRAYNEEKRENFFVTIGRIERGKMLENSILLSAKSGVKGVIIGSLNEKDYLKKLINLKRELNADIEIITNLPREELLKVLSKAKVYFHATIGEHFGVPVVEAMASGVIPIVPKESGAYEVVPEFSYSDIEEAVTLLKSLLEKENVELRRELKDRALNFSKENFKRKIFNEISSIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXR1</accession>
    <name>Q7LXR1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0637</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90211">
        <property type="entry name" value="B90211"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991171.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXR1"/>
    <dbReference type="STRING" id="273057.SSO0637"/>
    <dbReference type="PaxDb" id="273057-SSO0637"/>
    <dbReference type="EnsemblBacteria" id="AAK40945">
        <property type="protein sequence ID" value="AAK40945"/>
        <property type="gene ID" value="SSO0637"/>
    </dbReference>
    <dbReference type="GeneID" id="1454912"/>
    <dbReference type="KEGG" id="sso:SSO0637"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.641"/>
    <dbReference type="eggNOG" id="arCOG05996">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727316_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXR1"/>
    <dbReference type="PhylomeDB" id="Q7LXR1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="151" mass="17057" checksum="B3F3F667629579F9" modified="2011-05-31" version="1">MKQTDIKSLVNYVALKILGGSDYLIEALEEYLVKGEGPATVAYKYNISKHQLRGYAQRIIEKSGSEGKAKKLVPILKAISEGITPIVKKENDGSYECSLCNIIIAKEDSEEHIRKYHKEILNENINIMMQRLEKIKKDLQQNKAVIFTSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXX4</accession>
    <name>Q7LXX4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISC1359</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90197">
        <property type="entry name" value="C90197"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923018.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXX4"/>
    <dbReference type="PaxDb" id="273057-SSO0515"/>
    <dbReference type="EnsemblBacteria" id="AAK40834">
        <property type="protein sequence ID" value="AAK40834"/>
        <property type="gene ID" value="SSO0515"/>
    </dbReference>
    <dbReference type="GeneID" id="1455644"/>
    <dbReference type="KEGG" id="sso:SSO0515"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.510"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067987_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXX4"/>
    <dbReference type="PhylomeDB" id="Q7LXX4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033541">
        <property type="entry name" value="transpos_ISH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="89"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="363" mass="41984" checksum="AB2714FC26052DDB" modified="2011-05-31" version="1">MKHENTTKPAKFNRDFARSALKIIYSVLTKILFPEELLSALLKASGSYLSRLGKDGRRALRKLNAVQVEDVRDALRKMGRMTLRGVRDRRVAVDFHAIPQYHADKSFLSRIKPTKGTSWGLVQAAIFLLGRTRSFLDVIPVTVKNVAEGFKAVMEVIVKELEEDKLRLVMVFADREFAVNEVIRFLLELGLDFVISAKAQMYKKYKGMLQDVDVSFGGVRYTGFLCVRHGSGAYLIILRKEDGKIIAFLVRKEMDLYDAIVLAEMYRERWGIENAFRSLEEFRIRTRTCDVRKELVLVLLSYLLLNVWFLIRSWRKVKLWEFSVSLSNLLDREVRVEQERAFREVKTSFPQTPANPMHLLPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXY5</accession>
    <name>Q7LXY5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2192 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75380">
        <property type="entry name" value="S75380"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988928.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXY5"/>
    <dbReference type="STRING" id="273057.SSO0109"/>
    <dbReference type="PaxDb" id="273057-SSO0109"/>
    <dbReference type="EnsemblBacteria" id="AAK40464">
        <property type="protein sequence ID" value="AAK40464"/>
        <property type="gene ID" value="SSO0109"/>
    </dbReference>
    <dbReference type="GeneID" id="1453292"/>
    <dbReference type="KEGG" id="sso:SSO0109"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.105"/>
    <dbReference type="eggNOG" id="arCOG04166">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093718_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXY5"/>
    <dbReference type="PhylomeDB" id="Q7LXY5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018693">
        <property type="entry name" value="DUF2192"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09958">
        <property type="entry name" value="DUF2192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="26580" checksum="A5E18F0152B39C7F" modified="2011-05-31" version="1">MVKEIYRERIKVLTDIWSLLIESWESLSREDVIEIMKNAYVKKNIKPFRGFNADGLYEKELVSLFVVGKYGLGLFEDNKEIFDKLLSKEEYYDEISKLIISGNVNEAFEESGSNKEVLARALRTIFTKTVFSFESEDKLYKSLKNLNISDKDEIKHTSRSFSRFYTAFKLAESIAEGIVRDRLTYIATKKAIAISIGIEYPLPKADYVALIAKEVFNVNKKVLNKVLGVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXZ5</accession>
    <name>Q7LXZ5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1122 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75403">
        <property type="entry name" value="S75403"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988899.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXZ5"/>
    <dbReference type="STRING" id="273057.SSO0085"/>
    <dbReference type="PaxDb" id="273057-SSO0085"/>
    <dbReference type="EnsemblBacteria" id="AAK40445">
        <property type="protein sequence ID" value="AAK40445"/>
        <property type="gene ID" value="SSO0085"/>
    </dbReference>
    <dbReference type="GeneID" id="1453273"/>
    <dbReference type="KEGG" id="sso:SSO0085"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.84"/>
    <dbReference type="eggNOG" id="arCOG01699">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121769_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXZ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008304">
        <property type="entry name" value="UCP017998"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06557">
        <property type="entry name" value="DUF1122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017998">
        <property type="entry name" value="UCP017998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="22761" checksum="691A844BA6A0B811" modified="2011-05-31" version="1">MSELNGVNLSAFYKLEVRNLKNTHIKELVSFDLLLSNGRQIGKCNYFKGRDYYTPWLEIDYYPILRNENLEVIFFKVVYNFLSPGGKLFVTYIRDNETRERLYIGKHPVETPLGFSLLSAGFTWFKDWYFPEGGNEGFPKLQANKPINSDEGIRQLETIREEIKNVNVIRKIDELIDHYRKSRDRTVYWEIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TU6</accession>
    <name>Q97TU6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transposase ISC1234</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1377</name>
        <name evidence="3" type="ordered locus">SSO1420</name>
        <name evidence="4" type="ordered locus">SSO1481</name>
        <name evidence="5" type="ordered locus">SSO1616</name>
        <name evidence="6" type="ordered locus">SSO1883</name>
        <name evidence="7" type="ordered locus">SSO2567</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90321">
        <property type="entry name" value="H90321"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923364.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TU6"/>
    <dbReference type="STRING" id="273057.SSO1377"/>
    <dbReference type="PaxDb" id="273057-SSO1377"/>
    <dbReference type="EnsemblBacteria" id="AAK41611">
        <property type="protein sequence ID" value="AAK41611"/>
        <property type="gene ID" value="SSO1377"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41654">
        <property type="protein sequence ID" value="AAK41654"/>
        <property type="gene ID" value="SSO1420"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41706">
        <property type="protein sequence ID" value="AAK41706"/>
        <property type="gene ID" value="SSO1481"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41831">
        <property type="protein sequence ID" value="AAK41831"/>
        <property type="gene ID" value="SSO1616"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK42075">
        <property type="protein sequence ID" value="AAK42075"/>
        <property type="gene ID" value="SSO1883"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK42693">
        <property type="protein sequence ID" value="AAK42693"/>
        <property type="gene ID" value="SSO2567"/>
    </dbReference>
    <dbReference type="GeneID" id="1454610"/>
    <dbReference type="KEGG" id="sso:SSO1377"/>
    <dbReference type="KEGG" id="sso:SSO1420"/>
    <dbReference type="KEGG" id="sso:SSO1481"/>
    <dbReference type="KEGG" id="sso:SSO1616"/>
    <dbReference type="KEGG" id="sso:SSO1883"/>
    <dbReference type="KEGG" id="sso:SSO2567"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1382"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075479_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="319" mass="36774" checksum="F1791BE93F677CBB" modified="2001-10-01" version="1">MGVEGSTMARTLRHIPNLMYYPLPPIEDMPWREKWLTEIKPVLDAMDLERVLGEGALVYLKLLIVMVLYSCSYRDAVKMVNVNVVVAWFVGRKVGKSTLHDFVGRLYGVRKKLLEISFKLEEKCLPSYLPASAHLVDFMAWLVDSFLLDLPPGKRSVETFREKAELERREGNLERARKLLSLGRTKRRFEGRWTKKRGVSHYGLKAIAVISVSLFVRSITVKPANFSDKRFKSPLKGIKIADRGFSPSPTQLIAREKPFTTLRAHVEFFGTYLNAFWRPYGTTTWRNDVFLHVLGVIYNIKMFLAIQRRTPPGRRAVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U01</accession>
    <name>Q97U01_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Polysaccharide biosynthesis protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90508">
        <property type="entry name" value="D90508"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U01"/>
    <dbReference type="STRING" id="273057.SSO3228"/>
    <dbReference type="PaxDb" id="273057-SSO3228"/>
    <dbReference type="EnsemblBacteria" id="AAK43323">
        <property type="protein sequence ID" value="AAK43323"/>
        <property type="gene ID" value="SSO3228"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3228"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3330"/>
    <dbReference type="eggNOG" id="arCOG07183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1269984_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U01"/>
    <dbReference type="PhylomeDB" id="Q97U01"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="24208" checksum="67BF13686B6CEBCE" modified="2001-10-01" version="1">MYVFTIGYFTIPANLFSLITSRYSAERKNVGGIVIINLIAGISSSLVFALLVPYYISLTKLDVPIYFYSAISLIILTYLLTVTNSIAQGRTPKIVGISALIFQVLRLVSGIIFMFIFNLSILAVILAYSIGYLAQVLFNLFFIRANLKIDFKVAFSAVRKAVVYIANYIQYILEASIVWITLGIVNSDLVVAYFESAIIVSNIQIGHKQYIQGLWLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-10-18" modified="2025-06-18" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U03</accession>
    <name>Y3226_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized aldolase SSO3226</fullName>
            <ecNumber>4.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO3226</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the DeoC/FbaB aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.-"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90508">
        <property type="entry name" value="B90508"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924169.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U03"/>
    <dbReference type="SMR" id="Q97U03"/>
    <dbReference type="FunCoup" id="Q97U03">
        <property type="interactions" value="199"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3226"/>
    <dbReference type="PaxDb" id="273057-SSO3226"/>
    <dbReference type="EnsemblBacteria" id="AAK43321">
        <property type="protein sequence ID" value="AAK43321"/>
        <property type="gene ID" value="SSO3226"/>
    </dbReference>
    <dbReference type="GeneID" id="1453240"/>
    <dbReference type="KEGG" id="sso:SSO3226"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3328"/>
    <dbReference type="eggNOG" id="arCOG04044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057069_2_1_2"/>
    <dbReference type="InParanoid" id="Q97U03"/>
    <dbReference type="PhylomeDB" id="Q97U03"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00958">
        <property type="entry name" value="DhnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050456">
        <property type="entry name" value="DeoC/FbaB_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041720">
        <property type="entry name" value="FbaB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916:SF1">
        <property type="entry name" value="3-HYDROXY-5-PHOSPHONOOXYPENTANE-2,4-DIONE THIOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE CLASS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038992">
        <property type="entry name" value="Aldolase_Ia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000138960" description="Uncharacterized aldolase SSO3226">
        <location>
            <begin position="1"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="272" mass="29916" checksum="875B68CDCD2057E3" modified="2001-10-18" version="1">MIGSEIRMAKLFDKGRALVVALDHGLVMGPLKGIENPVEVVAKIAKNGPDALQMTPSMVKLVKENFFSRGSPMLITRLDTANVWRQKYKVYNEGYYASIYTVKDAISAGADAVVTYLVVGYGNDTVEGYNLSVLSSLRKEANDYGIPFIVEPLYVTKDNPDSVKEVDLVKYVTRLASEIGADILKVDYTGNKESFRQVINVAFSPILIRGGPKTNTTEEFLRMLRDALEAGAKGVTVGRNLWQAEEPDKLAKAISKVIHENADIGEALKILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U34</accession>
    <name>Q97U34_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99504">
        <property type="entry name" value="A99504"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991699.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U34"/>
    <dbReference type="STRING" id="273057.SSO3188"/>
    <dbReference type="PaxDb" id="273057-SSO3188"/>
    <dbReference type="EnsemblBacteria" id="AAK43288">
        <property type="protein sequence ID" value="AAK43288"/>
        <property type="gene ID" value="SSO3188"/>
    </dbReference>
    <dbReference type="GeneID" id="1453207"/>
    <dbReference type="KEGG" id="sso:SSO3188"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3293"/>
    <dbReference type="eggNOG" id="arCOG02099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2010190_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U34"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038723">
        <property type="entry name" value="ArnR1-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14947">
        <property type="entry name" value="HTH_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArnR1-like winged helix-turn-helix" evidence="1">
        <location>
            <begin position="56"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="123" mass="14151" checksum="62B36A754A71180A" modified="2001-10-01" version="1">MIIKKSVVFAISITLFHIGLLISKHHNYRPSSCPFLVYVPLTSNFIMDAKCRYNLLKILELVQEAEDGLTVEYISTKLNLSRKSVRTYLSKLESNGLIKRIDDIYKITEEGRKLIESLKLNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U40</accession>
    <name>Q97U40_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO3183</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90503">
        <property type="entry name" value="C90503"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991706.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U40"/>
    <dbReference type="STRING" id="273057.SSO3183"/>
    <dbReference type="PaxDb" id="273057-SSO3183"/>
    <dbReference type="EnsemblBacteria" id="AAK43282">
        <property type="protein sequence ID" value="AAK43282"/>
        <property type="gene ID" value="SSO3183"/>
    </dbReference>
    <dbReference type="GeneID" id="1453201"/>
    <dbReference type="KEGG" id="sso:SSO3183"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3287"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_16_2_2"/>
    <dbReference type="InParanoid" id="Q97U40"/>
    <dbReference type="PhylomeDB" id="Q97U40"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="130" mass="14194" checksum="4302DF5FA8D16E0D" modified="2001-10-01" version="1">MKVVIGYDGSDHAKKALFFTLNLIKKEDEIHLVTIVKEAPRSPEQVIIQSEQRAKQMQDEVVNELGDYKIVQKILESNEVADSILQYCNNIGCGLIVTGSRGLTGIKKAILGSVSSALVSKSNVPVLVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U41</accession>
    <name>Q97U41_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Serine/threonine protein kinase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO3182</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90503">
        <property type="entry name" value="B90503"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991707.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U41"/>
    <dbReference type="STRING" id="273057.SSO3182"/>
    <dbReference type="PaxDb" id="273057-SSO3182"/>
    <dbReference type="EnsemblBacteria" id="AAK43281">
        <property type="protein sequence ID" value="AAK43281"/>
        <property type="gene ID" value="SSO3182"/>
    </dbReference>
    <dbReference type="GeneID" id="1453200"/>
    <dbReference type="KEGG" id="sso:SSO3182"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3286"/>
    <dbReference type="eggNOG" id="arCOG03682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_430630_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U41"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14014">
        <property type="entry name" value="STKc_PknB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036321">
        <property type="entry name" value="ST1625"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101424">
        <property type="entry name" value="Hypothetical protein ST1625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="66"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="336"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="635" mass="71866" checksum="CFDFB25524AD49D8" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U75</accession>
    <name>Q97U75_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Third ORF in partial transposon ISC1160</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO12063</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90498">
        <property type="entry name" value="G90498"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U75"/>
    <dbReference type="PaxDb" id="273057-SSO12063"/>
    <dbReference type="EnsemblBacteria" id="AAK43246">
        <property type="protein sequence ID" value="AAK43246"/>
        <property type="gene ID" value="SSO12063"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO12063"/>
    <dbReference type="eggNOG" id="arCOG07929">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2490608_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U75"/>
    <dbReference type="PhylomeDB" id="Q97U75"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="86" mass="10169" checksum="3E99E7EEFC1ED9DD" modified="2001-10-01" version="1">MVFRLYSKVNPIETSYRNIKSFLPFISSTKFAFRKLIFMIALYFLPRWQKITLLPQSTLLEAKHVVPLHHIQGCEEGRLLLILLLH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2014-10-29" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U96</accession>
    <name>ARAD_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Arabinonate dehydratase</fullName>
            <ecNumber evidence="2">4.2.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">araD</name>
        <name evidence="5" type="ordered locus">SSO3124</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="J. Biol. Chem." volume="281" first="27378" last="27388">
            <title>Identification of the missing links in prokaryotic pentose oxidation pathways: evidence for enzyme recruitment.</title>
            <authorList>
                <person name="Brouns S.J."/>
                <person name="Walther J."/>
                <person name="Snijders A.P."/>
                <person name="van de Werken H.J."/>
                <person name="Willemen H.L."/>
                <person name="Worm P."/>
                <person name="de Vos M.G."/>
                <person name="Andersson A."/>
                <person name="Lundgren M."/>
                <person name="Mazon H.F."/>
                <person name="van den Heuvel R.H."/>
                <person name="Nilsson P."/>
                <person name="Salmon L."/>
                <person name="de Vos W.M."/>
                <person name="Wright P.C."/>
                <person name="Bernander R."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="16849334"/>
            <dbReference type="DOI" id="10.1074/jbc.M605549200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>INDUCTION BY D-ARABINOSE</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the dehydration of D-arabinonate to 2-keto-3-deoxy-D-arabinonate. Participates in a pentose oxidation pathway that converts D-arabinonate to 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>D-arabinonate = 2-dehydro-3-deoxy-D-arabinonate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21836"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16157"/>
            <dbReference type="ChEBI" id="CHEBI:16699"/>
            <dbReference type="EC" id="4.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Inhibited by substrate levels above 8 mM.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="2">Optimum pH is 6.7.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 85 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homooctamer.</text>
    </comment>
    <comment type="induction">
        <text evidence="2">Expression is strongly increased by growth on D-arabinose, both at the mRNA and at the protein level.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the mandelate racemase/muconate lactonizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.5" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90496">
        <property type="entry name" value="B90496"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989946.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U96"/>
    <dbReference type="SMR" id="Q97U96"/>
    <dbReference type="FunCoup" id="Q97U96">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3124"/>
    <dbReference type="PaxDb" id="273057-SSO3124"/>
    <dbReference type="DNASU" id="1453144"/>
    <dbReference type="EnsemblBacteria" id="AAK43225">
        <property type="protein sequence ID" value="AAK43225"/>
        <property type="gene ID" value="SSO3124"/>
    </dbReference>
    <dbReference type="GeneID" id="1453144"/>
    <dbReference type="GeneID" id="44128843"/>
    <dbReference type="KEGG" id="sso:SSO3124"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3230"/>
    <dbReference type="eggNOG" id="arCOG01168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_3_0_2"/>
    <dbReference type="InParanoid" id="Q97U96"/>
    <dbReference type="PhylomeDB" id="Q97U96"/>
    <dbReference type="BioCyc" id="MetaCyc:MONOMER-13205"/>
    <dbReference type="BRENDA" id="4.2.1.5">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047675">
        <property type="term" value="F:arabinonate dehydratase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019571">
        <property type="term" value="P:D-arabinose catabolic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd03316">
        <property type="entry name" value="MR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053670">
        <property type="entry name" value="MR_MLE-like_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046945">
        <property type="entry name" value="RHMD-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040866">
        <property type="entry name" value="AraD_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794">
        <property type="entry name" value="ENOLASE SUPERFAMILY, MANDELATE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794:SF58">
        <property type="entry name" value="MITOCHONDRIAL ENOLASE SUPERFAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00156">
        <property type="entry name" value="D-arabinonate_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00179">
        <property type="entry name" value="mandelate_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000430860" description="Arabinonate dehydratase">
        <location>
            <begin position="1"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P11444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16849334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="16849334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="373" mass="42437" checksum="5060B5D2ACAF238B" modified="2001-10-01" version="1">MIKDIRTYKLCYEGINDERDALAIKGLAEHPMEIVATEIETSDGYVGYGESLAYGCSDAVQVTIEKILKPLLLKEDEELIEYLWDKMYKATLRFGRRGIAIAGISGVDTALWDIMGKKAKKPIYKLLGGSKRKVRAYITGGYYSEKKDLEKLRDEEAYYVKMGFKGIKVKIGAKSMEEDIERLKAIREVVGEDVKIAVDANNVYTFEEALEMGRRLEKLGIWFFEEPIQTDYLDLSARLAEELEVPIAGYETAYTRWEFYEIMRKRAVDIVQTDVMWTGGISEMMKIGNMAKVMGYPLIPHYSAGGISLIGNLHVAAALNSPWIEMHLRKNDLRDKIFKESIEIDNGHLVVPDRPGLGYTIRDGVFEEYKCKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UD3</accession>
    <name>Q97UD3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90491">
        <property type="entry name" value="E90491"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989104.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UD3"/>
    <dbReference type="STRING" id="273057.SSO3084"/>
    <dbReference type="PaxDb" id="273057-SSO3084"/>
    <dbReference type="EnsemblBacteria" id="AAK43188">
        <property type="protein sequence ID" value="AAK43188"/>
        <property type="gene ID" value="SSO3084"/>
    </dbReference>
    <dbReference type="GeneID" id="15298664"/>
    <dbReference type="KEGG" id="sso:SSO3084"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3190"/>
    <dbReference type="eggNOG" id="arCOG02076">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2044514_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UD3"/>
    <dbReference type="PhylomeDB" id="Q97UD3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655">
        <property type="entry name" value="CONSERVED WITHIN P. AEROPHILUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655:SF2">
        <property type="entry name" value="PEROXIREDOXIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DsrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="120" mass="13467" checksum="64E5074B2549D5F4" modified="2001-10-01" version="1">MSDLYAIMVISGEDEKLYMAYITAIGYASSGNKVYMFFTMDGLRGVTKEIEKIQLPNVKPLSYYYENLIELGGDDIELTACEFGMKVKNVNHERLLDKVKISGVSEFALKSSEAKATLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UF3</accession>
    <name>Q97UF3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Arabinose ABC transporter, permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO3068</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99489">
        <property type="entry name" value="A99489"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990874.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UF3"/>
    <dbReference type="FunCoup" id="Q97UF3">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3068"/>
    <dbReference type="TCDB" id="3.A.1.1.14">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3068"/>
    <dbReference type="EnsemblBacteria" id="AAK43168">
        <property type="protein sequence ID" value="AAK43168"/>
        <property type="gene ID" value="SSO3068"/>
    </dbReference>
    <dbReference type="GeneID" id="15298680"/>
    <dbReference type="KEGG" id="sso:SSO3068"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3168"/>
    <dbReference type="eggNOG" id="arCOG00159">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_2_2"/>
    <dbReference type="InParanoid" id="Q97UF3"/>
    <dbReference type="PhylomeDB" id="Q97UF3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054927">
        <property type="entry name" value="GlcU_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040932">
        <property type="entry name" value="ABC_arch_GlcU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43879">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43879:SF1">
        <property type="entry name" value="GLUCOSE IMPORT SYSTEM PERMEASE PROTEIN GLCU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="83"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="295" mass="32545" checksum="17A395F8A5A03692" modified="2001-10-01" version="1">MERSVSKGSYITSSIKYALLEIIAAILVIMWLVPLYAMILGGLKSNLEAASTPILLPPSKPSLDAYAFAWFGYATIPGLEPTLLRYLLVAIPSVLLSVVIGTMTAYFFFVLSEKHGIISNGLFSIMALATFLPIETVTFPLIELETSLNVYNTYIGLIFAMLIFYVPTSALLMSIFLPVVPKYLIESARMDGAGDWTILWRVVFPLIFPGFLSTLIFVFLQIWNEFFIPLILTNTPNMLMLPVAARFYTAAYALIYNRSFAAGVISSLIPLIIFIFLGRYFIRGLAALGGGAKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UN8</accession>
    <name>Q97UN8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Na(+)/H(+) antiporter, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2965</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90476">
        <property type="entry name" value="G90476"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UN8"/>
    <dbReference type="FunCoup" id="Q97UN8">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2965"/>
    <dbReference type="PaxDb" id="273057-SSO2965"/>
    <dbReference type="EnsemblBacteria" id="AAK43070">
        <property type="protein sequence ID" value="AAK43070"/>
        <property type="gene ID" value="SSO2965"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2965"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3056"/>
    <dbReference type="eggNOG" id="arCOG01969">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_460525_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UN8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42751">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER FAMILY/TRKA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42751:SF3">
        <property type="entry name" value="SODIUM_GLUTAMATE SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="447"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="498"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="8">
        <location>
            <begin position="13"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK43070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="599" mass="68431" checksum="DF5F056D43DCB4D1" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97US2</accession>
    <name>Q97US2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2922</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="G90471"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924038.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97US2"/>
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    <dbReference type="EnsemblBacteria" id="AAK43030">
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        <property type="gene ID" value="SSO2922"/>
    </dbReference>
    <dbReference type="GeneID" id="1452949"/>
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    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:transposase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Transposase_11"/>
    </dbReference>
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        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="80"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37482" checksum="D4BBC709473D276A" modified="2001-10-01" version="1">MQVYETGFKTELKSLASYFIVNNLNVISQELDTRIADAAPFLLTENRGKEYLKVVREGEKVMSQGERSFKFYPHVKVEGETTIVAVDETAITVGEKESRKVEGFQLYNGKRKGVKLRSVDLVFPLRLSLLEEIADLRSDTPSQFLMRAVSEVAQHLKIDYVVADAGFLNLKVIKEMPVKTVVGGKTNLKGFKELSNVPLTEKKCEVNDRVYVAYRVLKYNDLYYYEVIYVKEKPRHFIFVTNFNGDPYKLAELYRLRWQSEEGFKVRKARIRYVRKLTNKIFLYLYYTVLDSVWNLVNYLLFNFKSTKRKPLSFNSFVRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97US3</accession>
    <name>Q97US3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2921</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43029.1"/>
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    </dbReference>
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        <property type="entry name" value="F90471"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924037.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97US3"/>
    <dbReference type="PaxDb" id="273057-SSO2921"/>
    <dbReference type="EnsemblBacteria" id="AAK43029">
        <property type="protein sequence ID" value="AAK43029"/>
        <property type="gene ID" value="SSO2921"/>
    </dbReference>
    <dbReference type="GeneID" id="1452948"/>
    <dbReference type="KEGG" id="sso:SSO2921"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3010"/>
    <dbReference type="eggNOG" id="arCOG07334">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_548170_0_0_2"/>
    <dbReference type="InParanoid" id="Q97US3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="497" mass="57197" checksum="676BD1CD88D16D2D" modified="2001-10-01" version="1">MQSTNLLDVATNVATLMFRANPNANFVRYQSPSFQTSIYSIYLMMKLGDFKSNFISFFSKYFSQNIILTIWNLVNNYALDDIIRIILYSIFIYTNLQNIPNTYIQKINNFSSFLYFAPKSTISVTEFHGGLSKSSNYYFLIDGNNLQHLMAFAVPGSVQKIGFKNPEVLYNVYINDIYDKCVVEFSSSNSGPFSNIEVFPAIDLVLPFDLRRASRKPITYLNNFNFKWITTNEMSFLRKQWTQVYMQMINSIKSISSTLSSLLKYPPIFPSLLNIQMAGINFPLSFLIPYNINARQKSLEGLMKEIHQIWIMLQIITYLKNQARLKLLNLDFSQSSSNPIAIFSCNGQDCSLWYEFDMNPHTMCRGLLWNLNSGPSWLENFYQRVTKCINSSTVTSIPLRPDIVILRGAKNCQDILSNGLSVEIVIECKNQQYKFWSNNIKTQILPYKCIFNPNKMILASMEQIPNIIKTQLSNNGVIAIDLVEPNNNGITQLLKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UU9</accession>
    <name>Q97UU9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2893</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90468">
        <property type="entry name" value="B90468"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UU9"/>
    <dbReference type="PaxDb" id="273057-SSO2893"/>
    <dbReference type="EnsemblBacteria" id="AAK43001">
        <property type="protein sequence ID" value="AAK43001"/>
        <property type="gene ID" value="SSO2893"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2893"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2981"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2285181_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UU9"/>
    <dbReference type="PhylomeDB" id="Q97UU9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="101" mass="11470" checksum="306A3504DF854289" modified="2001-10-01" version="1">MVMLSVLVTRRGLSLTVVSILLLNPVGRVLYREAYLGDAVLRRAFYLAALTAIKVNPIIKRFYEEHKENLKGKKLITACARKLAVITWAVLYYNKPFDASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V05</accession>
    <name>Q97V05_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Creatininase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2832</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90460">
        <property type="entry name" value="G90460"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990887.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V05"/>
    <dbReference type="SMR" id="Q97V05"/>
    <dbReference type="STRING" id="273057.SSO2832"/>
    <dbReference type="PaxDb" id="273057-SSO2832"/>
    <dbReference type="DNASU" id="1452861"/>
    <dbReference type="EnsemblBacteria" id="AAK42942">
        <property type="protein sequence ID" value="AAK42942"/>
        <property type="gene ID" value="SSO2832"/>
    </dbReference>
    <dbReference type="GeneID" id="1452861"/>
    <dbReference type="KEGG" id="sso:SSO2832"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2917"/>
    <dbReference type="eggNOG" id="arCOG04536">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055029_3_1_2"/>
    <dbReference type="InParanoid" id="Q97V05"/>
    <dbReference type="PhylomeDB" id="Q97V05"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10310">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024087">
        <property type="entry name" value="Creatininase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003785">
        <property type="entry name" value="Creatininase/forma_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35005:SF1">
        <property type="entry name" value="2-AMINO-5-FORMYLAMINO-6-RIBOSYLAMINOPYRIMIDIN-4(3H)-ONE 5'-MONOPHOSPHATE DEFORMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35005">
        <property type="entry name" value="3-DEHYDRO-SCYLLO-INOSOSE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02633">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102215">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="26484" checksum="B9110B882AE69329" modified="2001-10-01" version="1">MKLVEITREEIRGDLVGLVPVGSIEQHGPHLPMGTDGIISEYIASKAEEKLKDITLLFPTIYYGVSLEHKGFPFVSLSFQTAISFFTDLLESVFNDLGLRRIIIVNGHGGNSYFLPLVQREFNMKHSDAKVIVFNTFGEEEKILFNVNDLHAGTIETSKIWAIGKSLVRVNKIDEIKNYEVRDGVFIYYSSREANQFGVLNDGKPIRVNEEYGKKILRDLVDKLIWTILNFKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2024-03-27" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V23</accession>
    <name>PAT_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">N-acetyltransferase Pat</fullName>
            <shortName evidence="4 5">ssPat</shortName>
            <ecNumber evidence="2 3">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pat</name>
        <name evidence="7" type="ordered locus">SSO2813</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2005" name="J. Biol. Chem." volume="280" first="21122" last="21128">
            <title>Sir2 and the acetyltransferase, Pat, regulate the archaeal chromatin protein, Alba.</title>
            <authorList>
                <person name="Marsh V.L."/>
                <person name="Peak-Chew S.Y."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="15824122"/>
            <dbReference type="DOI" id="10.1074/jbc.m501280200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>MUTAGENESIS OF 89-GLY--GLY-91</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="journal article" date="2009" name="J. Biol. Chem." volume="284" first="19412" last="19419">
            <title>Structure and biochemical characterization of protein acetyltransferase from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Brent M.M."/>
                <person name="Iwata A."/>
                <person name="Carten J."/>
                <person name="Zhao K."/>
                <person name="Marmorstein R."/>
            </authorList>
            <dbReference type="PubMed" id="19473964"/>
            <dbReference type="DOI" id="10.1074/jbc.M109.014951"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS) IN COMPLEX WITH COENZYME A</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>MUTAGENESIS OF ASP-29; TYR-31; ARG-33; HIS-36; TYR-38; GLU-42; GLU-43; ASP-53; GLU-68; HIS-72; GLU-76; SER-78; TYR-113 AND MET-121</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">Modulates activity of albA1, the major archaeal DNA compaction protein, by decreasing albA1's nucleic acid binding affinity through acetylation of 'Lys-16'.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">580 uM for albA1 11-mer peptide VLIGKKPVMNY</KM>
            <KM evidence="3">48 uM for acetyl-CoA</KM>
            <text evidence="3">kcat is 2.31 min(-1).</text>
        </kinetics>
        <phDependence>
            <text evidence="3">Optimum pH is 7.0-9.0 at 75.0 degrees Celsius.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimal temperatures between 70 and 90 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the acetyltransferase family. GNAT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="2 3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90458">
        <property type="entry name" value="E90458"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989035.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3F8K">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.84 A"/>
        <property type="chains" value="A=1-160"/>
    </dbReference>
    <dbReference type="PDBsum" id="3F8K"/>
    <dbReference type="AlphaFoldDB" id="Q97V23"/>
    <dbReference type="SMR" id="Q97V23"/>
    <dbReference type="STRING" id="273057.SSO2813"/>
    <dbReference type="PaxDb" id="273057-SSO2813"/>
    <dbReference type="EnsemblBacteria" id="AAK42924">
        <property type="protein sequence ID" value="AAK42924"/>
        <property type="gene ID" value="SSO2813"/>
    </dbReference>
    <dbReference type="GeneID" id="1452843"/>
    <dbReference type="KEGG" id="sso:SSO2813"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2900"/>
    <dbReference type="eggNOG" id="arCOG00826">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691612_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V23"/>
    <dbReference type="PhylomeDB" id="Q97V23"/>
    <dbReference type="BRENDA" id="2.3.1.B27">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97V23"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050276">
        <property type="entry name" value="MshD_Acetyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43617">
        <property type="entry name" value="L-AMINO ACID N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43617:SF34">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000459687" description="N-acetyltransferase Pat">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 11">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 11">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 11">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 11">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 11">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 11">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 11">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 11">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 11">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Modestly reduces acetylation activity." evidence="3">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="29"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect on acetylation activity." evidence="3">
        <original>Y</original>
        <variation>S</variation>
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces acetylation activity, affinity to albA1 11-mer peptide and has no effect on acetyl-CoA affinity." evidence="3">
        <original>R</original>
        <variation>A</variation>
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect on acetylation activity." evidence="3">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces acetylation activity." evidence="3">
        <original>Y</original>
        <variation>S</variation>
        <location>
            <position position="38"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Modestly reduces acetylation activity." evidence="3">
        <original>E</original>
        <variation>Q</variation>
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Modestly reduces acetylation activity." evidence="3">
        <original>E</original>
        <variation>Q</variation>
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces acetylation activity." evidence="3">
        <original>D</original>
        <variation>N</variation>
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect on acetylation activity." evidence="3">
        <original>E</original>
        <variation>Q</variation>
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Modestly reduces acetylation activity. Modestly reduces acetylation activity, reduces affinity to albA1 11-mer peptide and has no effect on acetyl-CoA affinity; when associated with Q-76. Reduces acetylation activity; when associated with A-76." evidence="3">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces acetylation activity, affinity to albA1 11-mer peptide and has no effect on acetyl-CoA affinity. Reduces acetylation activity; when associated with A-72." evidence="3">
        <original>E</original>
        <variation>A</variation>
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect on acetylation activity. Modestly reduces acetylation activity, reduces affinity to albA1 11-mer peptide and has no effect on acetyl-CoA affinity; when associated with A-72." evidence="3">
        <original>E</original>
        <variation>Q</variation>
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect on acetylation activity." evidence="3">
        <original>S</original>
        <variation>A</variation>
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Modestly reduces acetylation activity." evidence="3">
        <original>S</original>
        <variation>C</variation>
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces acetylation activity." evidence="2">
        <original>GIG</original>
        <variation>AAA</variation>
        <location>
            <begin position="89"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect on acetylation activity." evidence="3">
        <original>Y</original>
        <variation>F</variation>
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces acetylation activity, affinity to albA1 11-mer peptide and has no effect on acetyl-CoA affinity." evidence="3">
        <original>M</original>
        <variation>H</variation>
        <variation>Y</variation>
        <location>
            <position position="121"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="6"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="helix" evidence="12">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="helix" evidence="12">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="helix" evidence="12">
        <location>
            <begin position="27"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="helix" evidence="12">
        <location>
            <begin position="35"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="54"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="64"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="75"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="helix" evidence="12">
        <location>
            <begin position="83"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="helix" evidence="12">
        <location>
            <begin position="90"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="108"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="helix" evidence="12">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="131"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="139"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15824122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="19473964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="15824122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="19473964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <evidence type="ECO:0000312" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="11">
        <source>
            <dbReference type="PDB" id="3F8K"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="12">
        <source>
            <dbReference type="PDB" id="3F8K"/>
        </source>
    </evidence>
    <sequence length="160" mass="18603" checksum="42B79B0D90720208" modified="2001-10-01" version="1">MNDQIKIRKATKEDWEKIYQLYNSLSDEDLYLRFFHLYRITEEDAKKIASNEDHVTFLAEVDGKVVGEASLHKDGEFSLVVHRNYRTLGIGTLLVKTLIEEAKKSGLSTVKFYTLPENTPMIKIGRKLGFKMRFYEDEVYGEMRLTERELNVNLATFSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V73</accession>
    <name>Q97V73_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase (Acd-5)</fullName>
            <ecNumber evidence="10">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">acd-5</name>
        <name evidence="10" type="ordered locus">SSO2761</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="10"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99452">
        <property type="entry name" value="A99452"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990244.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V73"/>
    <dbReference type="FunCoup" id="Q97V73">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2761"/>
    <dbReference type="PaxDb" id="273057-SSO2761"/>
    <dbReference type="EnsemblBacteria" id="AAK42872">
        <property type="protein sequence ID" value="AAK42872"/>
        <property type="gene ID" value="SSO2761"/>
    </dbReference>
    <dbReference type="GeneID" id="1452791"/>
    <dbReference type="KEGG" id="sso:SSO2761"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2842"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_2"/>
    <dbReference type="InParanoid" id="Q97V73"/>
    <dbReference type="PhylomeDB" id="Q97V73"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000004">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="16"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="131"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="239"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="398" mass="44364" checksum="91DA577F4B59DD43" modified="2001-10-01" version="1">MVFPFKSLEDFKVEITQDHELLRSAVRDFAENVLAKYVERGEKELDIPLEAKQKAKEIGLYGLFASQDLGGQGADYLSLLTATEEIARIWPSFSTFLLINWMFIYALSRFGNEELKKKYVIPVANGDKVAAFAVTEPVAGSDVAGIKTTAKKLDGEYVINGRKIFITNGDIADYYLIAARTSPPDPKARWKGISMFVVEKDAVKPVSRIETTGLRSSHTAEILLEDVKVQTENLLGEEGNGFKYAVSSFDFARTIVASQALGIGQAALEKMINYSLQRSAFEKKLAEFQLVQTKVSESLADLEVARLITYWAGTLFRNGKENEYTVAASLAKFISTESAERIILRAMSVYGGYGVTTSTGLERMLRDLQIMKTYEGTNDIQRISAARFLYNKFIGYKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V93</accession>
    <name>Q97V93_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2742</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90449">
        <property type="entry name" value="E90449"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V93"/>
    <dbReference type="FunCoup" id="Q97V93">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2742"/>
    <dbReference type="CAZy" id="GH15">
        <property type="family name" value="Glycoside Hydrolase Family 15"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2742"/>
    <dbReference type="DNASU" id="1452771"/>
    <dbReference type="EnsemblBacteria" id="AAK42852">
        <property type="protein sequence ID" value="AAK42852"/>
        <property type="gene ID" value="SSO2742"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2742"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2821"/>
    <dbReference type="eggNOG" id="arCOG03286">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010399_3_1_2"/>
    <dbReference type="InParanoid" id="Q97V93"/>
    <dbReference type="PhylomeDB" id="Q97V93"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053494">
        <property type="entry name" value="GH15_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045582">
        <property type="entry name" value="Trehalase-like_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041084">
        <property type="entry name" value="trehalase_H1_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616:SF0">
        <property type="entry name" value="GLUCAN 1,4-ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19291">
        <property type="entry name" value="TREH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trehalase-like N-terminal" evidence="3">
        <location>
            <begin position="7"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="GH15-like" evidence="2">
        <location>
            <begin position="209"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="581" mass="66379" checksum="D8E6F8EAD6B3FD83" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VA9</accession>
    <name>Q97VA9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90447">
        <property type="entry name" value="E90447"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990361.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VA9"/>
    <dbReference type="STRING" id="273057.SSO2725"/>
    <dbReference type="PaxDb" id="273057-SSO2725"/>
    <dbReference type="EnsemblBacteria" id="AAK42836">
        <property type="protein sequence ID" value="AAK42836"/>
        <property type="gene ID" value="SSO2725"/>
    </dbReference>
    <dbReference type="GeneID" id="1452755"/>
    <dbReference type="KEGG" id="sso:SSO2725"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2805"/>
    <dbReference type="eggNOG" id="arCOG01145">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041441_5_0_2"/>
    <dbReference type="InParanoid" id="Q97VA9"/>
    <dbReference type="PhylomeDB" id="Q97VA9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00838">
        <property type="entry name" value="MPP_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523:SF1">
        <property type="entry name" value="CALCINEURIN-LIKE PHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="324" mass="36626" checksum="B6DD51724764BA35" modified="2001-10-01" version="1">MGLFKRNNPDSNKIGGLKILFTSDLHGSETAFRKFLNAALMTKADTLIIGGDLAGKSLVPIIDIGNSKFKIEDKIVGKEGVDEIIKKYKSSGVYYTIVDQKGLTELNENRKSLDEEFKRVILERLQEWDRIAEEKLKGTNLKIYTNLGNDDPLYMFDVITKSERMVKCEGEIVNINGYEMITFGYVNPTPWNTPREMTEDQIYSNLKRIIEKVGNPAKTIFNLHAPPYGTNLDNAPLLDKTLKPVVKNGEIVMTHVGSMSIRKLEEEFQPLLGIHGHIHESRAFDKINKTIIINPGSEYNLGMLHAAYIILEDNKVKTHQFVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VB2</accession>
    <name>Q97VB2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2722</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99447">
        <property type="entry name" value="A99447"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VB2"/>
    <dbReference type="STRING" id="273057.SSO2722"/>
    <dbReference type="PaxDb" id="273057-SSO2722"/>
    <dbReference type="EnsemblBacteria" id="AAK42832">
        <property type="protein sequence ID" value="AAK42832"/>
        <property type="gene ID" value="SSO2722"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2722"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2803"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1302673_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VB2"/>
    <dbReference type="PhylomeDB" id="Q97VB2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="1">
        <location>
            <begin position="130"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="251" mass="29294" checksum="684CBB20A99FD5E3" modified="2001-10-01" version="1">MLWKEKDVRGKKTDFYDAEKLENMVNKAREYVNNPLKELVSLYLFMNDVQTKQENRLRRALFLVSDEEKLSEERLEKFSKGDFSDVKLYQLEYTGTVLREIQVLARTLLQTREELKEVRKMIEGNVPQDHVLLTIPGIGKLAAGIIIGIVGDIKRFPRPESFVAYCGLDPVVERSGRIEVRKGISKRGNKYLGSLFYFLAETQYSRNPTLLKFHEAHKDKLKGRKLYTALARKLARIVWSVWYNNKPYEVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VC4</accession>
    <name>Q97VC4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein 21A/21B N-terminal ARM repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2710</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90445">
        <property type="entry name" value="F90445"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988638.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VC4"/>
    <dbReference type="SMR" id="Q97VC4"/>
    <dbReference type="STRING" id="273057.SSO2710"/>
    <dbReference type="PaxDb" id="273057-SSO2710"/>
    <dbReference type="EnsemblBacteria" id="AAK42821">
        <property type="protein sequence ID" value="AAK42821"/>
        <property type="gene ID" value="SSO2710"/>
    </dbReference>
    <dbReference type="GeneID" id="1452740"/>
    <dbReference type="KEGG" id="sso:SSO2710"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2791"/>
    <dbReference type="eggNOG" id="arCOG03038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_920156_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VC4"/>
    <dbReference type="PhylomeDB" id="Q97VC4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056833">
        <property type="entry name" value="ARM_TT21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25062">
        <property type="entry name" value="ARM_TT21_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B N-terminal ARM repeat" evidence="5">
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            <begin position="10"/>
            <end position="204"/>
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            <begin position="31"/>
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            <begin position="110"/>
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        </location>
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            <begin position="173"/>
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        </location>
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            <begin position="207"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="10"/>
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        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="116"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="302" mass="36128" checksum="F5CDCD47C8E798C1" modified="2001-10-01" version="1">MSEILELINRGKYNEALKRIKEEIEQKKDDPELYYLMGLVYFKIGKYRRAIDNLRKAIRLKKDEAKYHCLLGYSLYMEGYNISDEKLIRRALKELQLSLEMDKDLPNKEKKYYYYMANILYELEDYEKALEKVNKALEIDDSSEVHKLRGDILFQLKKYNEAIEEYKTNLNDDKNLYAIAYTYFTMGKYDQALEYFNKAISANPEDPYYYQGKAETLLFMGRTNEAYNTIKRALEIDPDNPYIKLTEVEILTETNEKEAIKVLTEYLEEMEEFREIVCEEIREGKFKEKTKEVIEKVITKFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VE9</accession>
    <name>Q97VE9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2676</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
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        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoprotei</taxon>
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            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90442">
        <property type="entry name" value="D90442"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988596.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VE9"/>
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    <dbReference type="EnsemblBacteria" id="AAK42795">
        <property type="protein sequence ID" value="AAK42795"/>
        <property type="gene ID" value="SSO2676"/>
    </dbReference>
    <dbReference type="GeneID" id="1452714"/>
    <dbReference type="KEGG" id="sso:SSO2676"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2759"/>
    <dbReference type="eggNOG" id="arCOG07329">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1076113_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VE9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="87"/>
        </location>
    </feature>
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        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="254" mass="29426" checksum="DF901C9610F26FA9" modified="2001-10-01" version="1">MQDELVILSSIASIMVAIIVLILFYIEKIRTYVGVFFVYFSLTMMIAMFVGASIYLLYPSRVSLAIAFGINTFIMIPLIAYFLLKIAKLANTPFRGERTHVVIFSILLVLNEILMGTTFGIAQFGFSRFSNLYYSLYFSINSYWFFYPMMAEMLALYLLHYLKGSINRELFPLIGLATFPPTAFDYQEWFYSALIFSLAFSILGSILSKRLWRYVYLMLILSELILLINAIPYDIAIAISMILYYYQSLLPLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VG6</accession>
    <name>Q97VG6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Rieske iron-sulfur protein-1 (SoxL-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">soxL-1</name>
        <name evidence="8" type="ordered locus">SSO2660</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90440">
        <property type="entry name" value="C90440"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988572.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VG6"/>
    <dbReference type="STRING" id="273057.SSO2660"/>
    <dbReference type="PaxDb" id="273057-SSO2660"/>
    <dbReference type="EnsemblBacteria" id="AAK42778">
        <property type="protein sequence ID" value="AAK42778"/>
        <property type="gene ID" value="SSO2660"/>
    </dbReference>
    <dbReference type="GeneID" id="1452697"/>
    <dbReference type="KEGG" id="sso:SSO2660"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2742"/>
    <dbReference type="eggNOG" id="arCOG01720">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074282_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VG6"/>
    <dbReference type="PhylomeDB" id="Q97VG6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Rieske" evidence="7">
        <location>
            <begin position="166"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="324" mass="35682" checksum="3C42C5C11EA3D960" modified="2001-10-01" version="1">MARGIPIIKRDDLIFAIKLLRRMRDPKTRFDEKEFMKSGRDYLYNYAEKNVGPLDPSRRAFLKGILIGIGAAAVLSAIPVISYLNQPEISLKQFPWVIIVDSSGNPIKASQIPVNDPEILLFEYPMQGDITFILNLGDENDNPIKVPPTTVVIPENGKTYTFPGGVGPNNSIVAFSAICQHLGCTPPEIHFYPPKYMKVGLPTPNFLPQVALQAAQQANAPGVIHCDCHGSTYDPYHGASVLTGPTVRPLPYVQLYWDSNTDYLYAIGMNLNAPTILGRTNDLSGYAYLSSYDESTGCPKMLLQSGQTPSNCYTELESAGNTYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VI0</accession>
    <name>Q97VI0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycerol-3-phosphate dehydrogenase chain C (Anaerobic) (GlpC)</fullName>
            <ecNumber evidence="7">1.1.5.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">glpC</name>
        <name evidence="7" type="ordered locus">SSO2643</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.5.3" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90438">
        <property type="entry name" value="E90438"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988551.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VI0"/>
    <dbReference type="FunCoup" id="Q97VI0">
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    <dbReference type="EnsemblBacteria" id="AAK42764">
        <property type="protein sequence ID" value="AAK42764"/>
        <property type="gene ID" value="SSO2643"/>
    </dbReference>
    <dbReference type="GeneID" id="1452683"/>
    <dbReference type="KEGG" id="sso:SSO2643"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2726"/>
    <dbReference type="eggNOG" id="arCOG00343">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_023081_7_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:electron transport chain"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
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        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32479:SF19">
        <property type="entry name" value="ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32479">
        <property type="entry name" value="GLYCOLATE OXIDASE IRON-SULFUR SUBUNIT"/>
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    </dbReference>
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        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="4Fe-4S ferredoxins"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cysteine-rich" evidence="6">
        <location>
            <begin position="170"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Cysteine-rich" evidence="6">
        <location>
            <begin position="293"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="395" mass="45035" checksum="4A84EFCCE9D3C599" modified="2001-10-01" version="1">MYSLNPSNSTFFDSNKLLSEFIRQASVCHGCRLCFNYCDSFPLMFTYTDKKGPKNLTLDDLFNVASKCFHCKMCYVNCPYVPPHEFNMDFPSLMEWAWLYYKKNRGLTVRDFIFEMLDGVKFARPLAKVIMEKNKELLGIHKEAPTLPVAEKGLRERVKPKRIDSPKARVALFPTCLIENFFPEIGEDLVEIYNELGIEVIIPNFVCCGAPMLDSGDVDRLKKNAEYNIKIIEDLIKEGYDVVSPIPTCTLMIKEYKKVLDREVPKVYDAMEYLLKLKNEGKIELKGKIEKSVYYHPPCHLKFLQLGLPGVRLLRSMGAKVDISNNGCSGIDGGWGLRNYDTAKRVGSKMMEAFKQSKADLFSTECPLAGLQIEKSSGRRPLHPIQLLKEAMKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VI4</accession>
    <name>Q97VI4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbon monoxide dehydrogenase, large chain (CutA-4)</fullName>
            <ecNumber evidence="4">1.2.7.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cutA-4</name>
        <name evidence="4" type="ordered locus">SSO2639</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.4" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99438">
        <property type="entry name" value="A99438"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988546.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VI4"/>
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    </dbReference>
    <dbReference type="STRING" id="273057.SSO2639"/>
    <dbReference type="PaxDb" id="273057-SSO2639"/>
    <dbReference type="EnsemblBacteria" id="AAK42760">
        <property type="protein sequence ID" value="AAK42760"/>
        <property type="gene ID" value="SSO2639"/>
    </dbReference>
    <dbReference type="GeneID" id="1452679"/>
    <dbReference type="KEGG" id="sso:SSO2639"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2722"/>
    <dbReference type="eggNOG" id="arCOG01167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_2_0_2"/>
    <dbReference type="InParanoid" id="Q97VI4"/>
    <dbReference type="PhylomeDB" id="Q97VI4"/>
    <dbReference type="BioCyc" id="MetaCyc:MONOMER-18071"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:anaerobic carbon-monoxide dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Xanthine dehydrogenase oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
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    </dbReference>
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        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
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    </dbReference>
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        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
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        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
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        <property type="entry name" value="AldOxase/xan_DH_MoCoBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053554">
        <property type="entry name" value="Glyceraldehyde_dh-related"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041018">
        <property type="entry name" value="glyceraldDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908:SF132">
        <property type="entry name" value="ALDEHYDE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="MoCoBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20256">
        <property type="entry name" value="MoCoBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde oxidase/xanthine dehydrogenase a/b hammerhead" evidence="3">
        <location>
            <begin position="18"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="749" mass="83013" checksum="D2DE690F07CDAA02" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-11-27" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VI5</accession>
    <name>Q97VI5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Carbon monoxide dehydrogenase, small chain. Carboxy-end (CutC-2)</fullName>
            <ecNumber evidence="1">1.2.7.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cutC-2</name>
        <name evidence="1" type="ordered locus">SSO10802</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.4" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90437">
        <property type="entry name" value="H90437"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VI5"/>
    <dbReference type="PaxDb" id="273057-SSO10802"/>
    <dbReference type="EnsemblBacteria" id="AAK42759">
        <property type="protein sequence ID" value="AAK42759"/>
        <property type="gene ID" value="SSO10802"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO10802"/>
    <dbReference type="HOGENOM" id="CLU_2911715_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VI5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:anaerobic carbon-monoxide dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="61" mass="7473" checksum="998582347CEFB902" modified="2001-10-01" version="1">MISSTPTNVTVKSISNLLFRRIWIFFQQEICLHYHSRRTETTLKGEIFLKRLLNWMKLRIY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-05" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VP5</accession>
    <name>SFSA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sugar fermentation stimulation protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sfsA</name>
        <name type="ordered locus">SSO2571</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SfsA family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90430">
        <property type="entry name" value="C90430"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991792.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VP5"/>
    <dbReference type="SMR" id="Q97VP5"/>
    <dbReference type="STRING" id="273057.SSO2571"/>
    <dbReference type="PaxDb" id="273057-SSO2571"/>
    <dbReference type="EnsemblBacteria" id="AAK42698">
        <property type="protein sequence ID" value="AAK42698"/>
        <property type="gene ID" value="SSO2571"/>
    </dbReference>
    <dbReference type="GeneID" id="1454025"/>
    <dbReference type="GeneID" id="44128302"/>
    <dbReference type="KEGG" id="sso:SSO2571"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2653"/>
    <dbReference type="eggNOG" id="arCOG04115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052299_1_0_2"/>
    <dbReference type="InParanoid" id="Q97VP5"/>
    <dbReference type="PhylomeDB" id="Q97VP5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22357">
        <property type="entry name" value="SfsA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.580:FF:000002">
        <property type="entry name" value="Sugar fermentation stimulation protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00095">
        <property type="entry name" value="SfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005224">
        <property type="entry name" value="SfsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040452">
        <property type="entry name" value="SfsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041465">
        <property type="entry name" value="SfsA_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00230">
        <property type="entry name" value="sfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30545">
        <property type="entry name" value="SUGAR FERMENTATION STIMULATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30545:SF2">
        <property type="entry name" value="SUGAR FERMENTATION STIMULATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03749">
        <property type="entry name" value="SfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17746">
        <property type="entry name" value="SfsA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152328" description="Sugar fermentation stimulation protein homolog">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00095"/>
        </source>
    </evidence>
    <sequence length="240" mass="27830" checksum="A6452F004C3F4A79" modified="2001-10-01" version="1">MNESNSQDLTRVSPFYIKEGFSVYEFTEKLFEAYVIERINRFLVKVTLDGEEILVHLHDPGRLKELIYPGNLVLIRETKGNKTKFSITAAYANSRFIVLDSRLHNIIASKFIPKIYEKEVKVGNSRIDFKYDNTYLEVKGCTLVENEIAYFPDAPTERGRKHLKELRELMRKGFNAILLILVMRNDAKCFLPNEKTDPKFSVEFWDSIKEGLKVYIKTFSLIGNKIVYVGDIPLCKTNLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VR0</accession>
    <name>Q97VR0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90428">
        <property type="entry name" value="C90428"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990125.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VR0"/>
    <dbReference type="STRING" id="273057.SSO2553"/>
    <dbReference type="PaxDb" id="273057-SSO2553"/>
    <dbReference type="EnsemblBacteria" id="AAK42682">
        <property type="protein sequence ID" value="AAK42682"/>
        <property type="gene ID" value="SSO2553"/>
    </dbReference>
    <dbReference type="GeneID" id="1454009"/>
    <dbReference type="KEGG" id="sso:SSO2553"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2635"/>
    <dbReference type="eggNOG" id="arCOG01932">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_4_0_2"/>
    <dbReference type="InParanoid" id="Q97VR0"/>
    <dbReference type="PhylomeDB" id="Q97VR0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43383:SF2">
        <property type="entry name" value="AMIDOHYDROLASE 2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43383">
        <property type="entry name" value="NODULIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="48"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="339" mass="39583" checksum="E39F193CF71E331A" modified="2001-10-01" version="1">MSEKEFIMASAESWLENEISTDTFTMNSLRPFYLYLRNELKLLLGVNFIEERNKLIKNDPVNYMRLLFDDAGIDGFVIDTGFGKKEMEIPAKLKLLFRIESIINDDIFQMSFDKALEYFEETLRRKITKEGYAGFKSIIAYRTGLKVNCNIEEARRDFHSNEKDWFGKVAKGFRDYLLCETLRIAKELRVPVQIHTGAGDRDIKLELSRPSYLTNIVRKYEGKVIFVHAGYPYHRETAWMSYLFPSVYLDTSQVIPFAPLAAYNILNEIFEVAPLNKVMHGSDAFHIPEISWLAAKLAKKAIQKTTELMIEKNILDEKHAKEMVERFLYYNSKEMYGFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VS0</accession>
    <name>Q97VS0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposon ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2542</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90426">
        <property type="entry name" value="H90426"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923912.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VS0"/>
    <dbReference type="STRING" id="273057.SSO2542"/>
    <dbReference type="PaxDb" id="273057-SSO2542"/>
    <dbReference type="EnsemblBacteria" id="AAK42671">
        <property type="protein sequence ID" value="AAK42671"/>
        <property type="gene ID" value="SSO2542"/>
    </dbReference>
    <dbReference type="GeneID" id="1453998"/>
    <dbReference type="KEGG" id="sso:SSO2542"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2621"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066381_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VS0"/>
    <dbReference type="PhylomeDB" id="Q97VS0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="3">
        <location>
            <begin position="194"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="316" mass="35996" checksum="CCFC0FD1C808E95D" modified="2001-10-01" version="1">MEAPVAGIDVSKDKLVVYFQGKYYEFQNNKQGFEGVKQVLPKGCKVAIESTGVYHINLAKYLGKEYDVRIINPLVLKKFKNIRGKKSDKNDAKKLAELVISMGSEFTTSEAKELTSQWDFITRSIVRVKNRLRRDLILLGYKDSLSKKNLNEVLRGEDNIVLSEVRFLLDELERLEVRKREIEEELEDLVPKDSLIFTIPGIGRTLGCIILARVGDVKRFGDKKRFVAYCGLDPLVESSGKSVVSRGISRRGDAVLRRAFYLAALTAIKVNPIIKRFYEEHKENLKGKKLITACARKLAVITWAVLYYNKPFDANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VT0</accession>
    <name>Q97VT0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90425">
        <property type="entry name" value="E90425"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VT0"/>
    <dbReference type="STRING" id="273057.SSO2530"/>
    <dbReference type="PaxDb" id="273057-SSO2530"/>
    <dbReference type="EnsemblBacteria" id="AAK42660">
        <property type="protein sequence ID" value="AAK42660"/>
        <property type="gene ID" value="SSO2530"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2530"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2607"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123170_2_2_2"/>
    <dbReference type="InParanoid" id="Q97VT0"/>
    <dbReference type="PhylomeDB" id="Q97VT0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="10"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="130" mass="15165" checksum="5C987BDF28ACAFA6" modified="2001-10-01" version="1">MMREEAQKWFRQALEDLATAKDTITTGHYYASAFWAEQAAEKALKALLIENGKIERTHDLNQLLYVIKEEIGLPVEEIRSEVNKLTLHYTISRYPDAANTIPYSLYTKEDAEELVKKAEKVIEWVRRNLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VU4</accession>
    <name>Q97VU4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90423">
        <property type="entry name" value="G90423"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988488.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VU4"/>
    <dbReference type="FunCoup" id="Q97VU4">
        <property type="interactions" value="179"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2515"/>
    <dbReference type="PaxDb" id="273057-SSO2515"/>
    <dbReference type="EnsemblBacteria" id="AAK42646">
        <property type="protein sequence ID" value="AAK42646"/>
        <property type="gene ID" value="SSO2515"/>
    </dbReference>
    <dbReference type="GeneID" id="1453973"/>
    <dbReference type="KEGG" id="sso:SSO2515"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2592"/>
    <dbReference type="eggNOG" id="arCOG00498">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048478_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VU4"/>
    <dbReference type="PhylomeDB" id="Q97VU4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07725">
        <property type="entry name" value="TTHA1429-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050662">
        <property type="entry name" value="Sec-metab_biosynth-thioest"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23131">
        <property type="entry name" value="ENDORIBONUCLEASE LACTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23131:SF4">
        <property type="entry name" value="METALLO-BETA-LACTAMASE SUPERFAMILY POTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="310" mass="35985" checksum="13A92987D0A99905" modified="2001-10-01" version="1">MTIFTLKLPMQGPLKYINSYLVKGNEESVLIDTGLPTQEDVTTLSNYLKAYGYPNFVVITHYHPDHMGLVRLFKDKSIILISDRELEYVTYLLSEEYEKEMRKYLLANGFPEEFIQRMFRNRSRFSEIIDGVNFNTVKDGDVIKLGQEQMRVLLTPGHTMGHICLVYEKVVFCGDHILQDITPNISLLRLEDNPLKAYLESLDKVQKLDIELLYPAHGEVLKDVSKRVEEIKEHHKRRLEEIMNIIRTLGKTSGFNIASRISWYKKWDELSAFDKQLAMGETLAHIKYLVEEGFIREVNASGSIYYTMSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VV7</accession>
    <name>Q97VV7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ribonucleotide reductase small subunit, probable (NrdB)</fullName>
            <ecNumber evidence="1">1.17.4.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nrdB</name>
        <name evidence="1" type="ordered locus">SSO2498</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.17.4.1" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90422">
        <property type="entry name" value="B90422"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923901.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VV7"/>
    <dbReference type="FunCoup" id="Q97VV7">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2498"/>
    <dbReference type="PaxDb" id="273057-SSO2498"/>
    <dbReference type="EnsemblBacteria" id="AAK42633">
        <property type="protein sequence ID" value="AAK42633"/>
        <property type="gene ID" value="SSO2498"/>
    </dbReference>
    <dbReference type="GeneID" id="7808252"/>
    <dbReference type="KEGG" id="sso:SSO2498"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2578"/>
    <dbReference type="eggNOG" id="arCOG03658">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072736_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VV7"/>
    <dbReference type="PhylomeDB" id="Q97VV7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006198">
        <property type="entry name" value="PRK08326.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006199">
        <property type="entry name" value="PRK08326.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006200">
        <property type="entry name" value="PRK08326.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409:SF18">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SUBUNIT M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="308" mass="35632" checksum="ADA953927A669577" modified="2001-10-01" version="1">MGMSFEEYKHEYFKSIRSGGLNWSLFPMKLYQLGKKLFWDPANIDLSKDAEDWKKLNDLEKMFIINVGSKFAAGEEAVALDLHPLIVTLVKEGRVEEVMYLEQFVYEESKHVEAFRRFFDIVNVMEDLSAYTKDLSPNYKKIFYEELPKAMWNLSRDPSPENQVRAVVTYNLIVEGVAAEGGYNIFRQITNTRKILPGLAKMVNLIATDESRHIAFGIYLITRLVKEYGEGVYKAAMDHINYLAPYAIGIFSEPTMPQVESLPFNLTNIELVDYAKKLLNTRIDAINRARRMKLEMLLPKDLDVIESW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VZ5</accession>
    <name>Q97VZ5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Isochorismatase, putative (EntB-like2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">entB-like2</name>
        <name evidence="3" type="ordered locus">SSO2455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90417">
        <property type="entry name" value="D90417"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923863.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VZ5"/>
    <dbReference type="FunCoup" id="Q97VZ5">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2455"/>
    <dbReference type="PaxDb" id="273057-SSO2455"/>
    <dbReference type="EnsemblBacteria" id="AAK42595">
        <property type="protein sequence ID" value="AAK42595"/>
        <property type="gene ID" value="SSO2455"/>
    </dbReference>
    <dbReference type="GeneID" id="84060359"/>
    <dbReference type="KEGG" id="sso:SSO2455"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2533"/>
    <dbReference type="eggNOG" id="arCOG01943">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068979_8_4_2"/>
    <dbReference type="InParanoid" id="Q97VZ5"/>
    <dbReference type="PhylomeDB" id="Q97VZ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00431">
        <property type="entry name" value="cysteine_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050272">
        <property type="entry name" value="Isochorismatase-like_hydrls"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540:SF6">
        <property type="entry name" value="ISOCHORISMATASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540">
        <property type="entry name" value="PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="2">
        <location>
            <begin position="28"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="205" mass="23361" checksum="8685586899EDE032" modified="2001-10-01" version="1">MRAVNLIMKIEVPSIPEHKEVILDPTNTALIIVDMQNDFVRKNGKLSVPTAEATIPFIKRLVDKARSSNALVIYTQDWHMKDDPEFKIWGEHALAGTWGAEIIDELTPEKSDFIVKKYRYDAFFESSLDYILRVKNIKNTIITGTVANICVLHTAGSAALRWYNVIMPKDSISAITEFDYYATLRQVDFLYKGIITTADGIKFER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-03-01" modified="2025-06-18" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VZ9</accession>
    <name>GUAAA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GMP synthase [glutamine-hydrolyzing] subunit A</fullName>
            <ecNumber evidence="1">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">guaAA</name>
        <name type="synonym">guaA</name>
        <name type="ordered locus">SSO2451</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>XMP + L-glutamine + ATP + H2O = GMP + L-glutamate + AMP + diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90416">
        <property type="entry name" value="H90416"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923859.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VZ9"/>
    <dbReference type="SMR" id="Q97VZ9"/>
    <dbReference type="FunCoup" id="Q97VZ9">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2451"/>
    <dbReference type="MEROPS" id="C26.A31"/>
    <dbReference type="PaxDb" id="273057-SSO2451"/>
    <dbReference type="EnsemblBacteria" id="AAK42591">
        <property type="protein sequence ID" value="AAK42591"/>
        <property type="gene ID" value="SSO2451"/>
    </dbReference>
    <dbReference type="GeneID" id="12417133"/>
    <dbReference type="KEGG" id="sso:SSO2451"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2529"/>
    <dbReference type="eggNOG" id="arCOG00087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_1_4_2"/>
    <dbReference type="InParanoid" id="Q97VZ9"/>
    <dbReference type="PhylomeDB" id="Q97VZ9"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003922">
        <property type="term" value="F:GMP synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000047">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing] subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01510">
        <property type="entry name" value="GMP_synthase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023686">
        <property type="entry name" value="GMP_synthase_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001975">
        <property type="entry name" value="PRK00758.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0332">GMP biosynthesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140233" description="GMP synthase [glutamine-hydrolyzing] subunit A">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="2"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01510"/>
        </source>
    </evidence>
    <sequence length="188" mass="21035" checksum="DB7CC4CE03451481" modified="2001-10-01" version="1">MKVGLVYYGGQYNHLILKNVKYLGADIEVIPPHKPVEELKKFDCVIFSGGPYSVSEEIQKMGNSPLYIKELKVPMLGICLGHQLIAYVLGGVVRRALNPEYGLTRINIFDEDTILKGFSQQLNVWESHNDEVVEPPSGFRVLASSANARVQAMANSSNSIFGVQFHPEVKHTERGIEIFKNFLGVCRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-06-07" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WC7</accession>
    <name>CBIT_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)</fullName>
            <ecNumber evidence="1">2.1.1.196</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbiT</name>
        <name type="ordered locus">SSO2303</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylation of C-15 in cobalt-precorrin-6B followed by the decarboxylation of C-12 to form cobalt-precorrin-7.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Co-precorrin-6B + S-adenosyl-L-methionine = Co-precorrin-7 + S-adenosyl-L-homocysteine + CO2</text>
            <dbReference type="Rhea" id="RHEA:36067"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:70791"/>
            <dbReference type="ChEBI" id="CHEBI:72780"/>
            <dbReference type="EC" id="2.1.1.196"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 8/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. Archaeal-type CbiT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.196" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90400">
        <property type="entry name" value="F90400"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991808.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WC7"/>
    <dbReference type="SMR" id="Q97WC7"/>
    <dbReference type="FunCoup" id="Q97WC7">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2303"/>
    <dbReference type="PaxDb" id="273057-SSO2303"/>
    <dbReference type="EnsemblBacteria" id="AAK42461">
        <property type="protein sequence ID" value="AAK42461"/>
        <property type="gene ID" value="SSO2303"/>
    </dbReference>
    <dbReference type="GeneID" id="1453788"/>
    <dbReference type="GeneID" id="44128031"/>
    <dbReference type="KEGG" id="sso:SSO2303"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2393"/>
    <dbReference type="eggNOG" id="arCOG00977">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094143_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WC7"/>
    <dbReference type="PhylomeDB" id="Q97WC7"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00229"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043776">
        <property type="term" value="F:cobalt-precorrin-6B C5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00786">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023475">
        <property type="entry name" value="CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014008">
        <property type="entry name" value="Cbl_synth_MTase_CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050714">
        <property type="entry name" value="Cobalamin_biosynth_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02469">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001556">
        <property type="entry name" value="PRK00377.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182">
        <property type="entry name" value="COBALT-PRECORRIN-6B C(15)-METHYLTRANSFERASE (DECARBOXYLATING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-7 C(5)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000134944" description="Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="48"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00786"/>
        </source>
    </evidence>
    <sequence length="199" mass="21799" checksum="6DE834AA6C1C105F" modified="2001-10-01" version="1">MEWNYVIPGIPDNLFERDEEIPMTKEEIRALALSKLRIKKGDKVLDIGCGTGSITVEASLLVGNSGRVYGIDKEEKAINLTRRNAEKFGVLNNIVLIKGEAPAILSTINEKFDRIFIGGGSEKIKEIISASWEIINKGGRIVIDAILLETVNNALSAMEKIGFVNLEITEVIIAKGMKTKVGTAMMARNPIFIISGEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WI3</accession>
    <name>Q97WI3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2233</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90393">
        <property type="entry name" value="D90393"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992039.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WI3"/>
    <dbReference type="STRING" id="273057.SSO2233"/>
    <dbReference type="PaxDb" id="273057-SSO2233"/>
    <dbReference type="EnsemblBacteria" id="AAK42403">
        <property type="protein sequence ID" value="AAK42403"/>
        <property type="gene ID" value="SSO2233"/>
    </dbReference>
    <dbReference type="GeneID" id="1453730"/>
    <dbReference type="KEGG" id="sso:SSO2233"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2330"/>
    <dbReference type="eggNOG" id="arCOG00759">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_904936_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WI3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF289">
        <property type="entry name" value="GLYCINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="304" mass="33740" checksum="2E5079BC9236DD89" modified="2001-10-01" version="1">MTKLVIVGSGITGLITALKYGGDVIILEKNKIVAHNSKASLWSIIPPLCGNHREDCEKGIRFYEEICEKYGIYCKKTHVIRVSSKKLGGKIIDRKEIRSIEPQLDIEEAELFDAGLFVEGDELFNTLGTEFNVESNSEVTDIIVRDNEIKSLITSKGEIKGEFYIFATGYLTPKLFSKLGIEVNLFKGHLVITKKTSLNGILIVNDRIAVEGKNLYLNGDSKPNSSSAVDYDEISKTINEIANVLKIDTNNLEIRVGFRSVSKDGEPIVKKIYSNGILITGHRFGFALAPILAEKAIQMIKYGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WJ6</accession>
    <name>Q97WJ6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90391">
        <property type="entry name" value="G90391"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992060.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WJ6"/>
    <dbReference type="STRING" id="273057.SSO2221"/>
    <dbReference type="PaxDb" id="273057-SSO2221"/>
    <dbReference type="EnsemblBacteria" id="AAK42390">
        <property type="protein sequence ID" value="AAK42390"/>
        <property type="gene ID" value="SSO2221"/>
    </dbReference>
    <dbReference type="GeneID" id="1453717"/>
    <dbReference type="KEGG" id="sso:SSO2221"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2317"/>
    <dbReference type="eggNOG" id="arCOG05885">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168020_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WJ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="117" mass="13581" checksum="E7E43BEC5992B208" modified="2001-10-01" version="1">MTDYTQLNIVILAVPLPILTYLWFKYYNIIITEGTKYVGEKYREEEVLLLPSSTRTVKIDGKVSVLVYGVNPWITIRVNSGPKQKIFKIRLLNESGNLELINESKVFQVRVKLRYSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-11-27" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WQ3</accession>
    <name>Q97WQ3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99374">
        <property type="entry name" value="B99374"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988835.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WQ3"/>
    <dbReference type="STRING" id="273057.SSO2066"/>
    <dbReference type="PaxDb" id="273057-SSO2066"/>
    <dbReference type="EnsemblBacteria" id="AAK42249">
        <property type="protein sequence ID" value="AAK42249"/>
        <property type="gene ID" value="SSO2066"/>
    </dbReference>
    <dbReference type="GeneID" id="71027460"/>
    <dbReference type="KEGG" id="sso:SSO2066"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2144"/>
    <dbReference type="eggNOG" id="arCOG08425">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG08426">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_914056_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WQ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="304" mass="34853" checksum="9BEA1A1F4098C611" modified="2001-10-01" version="1">MAQTHEMLEKFLNNFLKLVIISELMNIDIDANNLDRLLKVSSDIAVDLSIGIARRKFGNDKTKKIKEGLSNAYLWLQSTPTTPELYYLLMDKLSRIVTEYNIDLAWKELEELENYLLELNKKKEVKDVQLGKSIETISDMKKILINYLASIQGEADEAFLIMRDEVFNLILGNNIYELKINGLSDNEKKSSDSFRLSNALKLLLDEEDKIRNKKLMGEVFTISKVKDKEGRIINVLISFATAKEILNLRESPNISVKLLRKVEDFCIFLSGSTLYGMVSCNVELDVNSVYTYKGENIFEKISQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WR6</accession>
    <name>Q97WR6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hemerythrin HHE cation binding domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90372">
        <property type="entry name" value="E90372"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WR6"/>
    <dbReference type="PaxDb" id="273057-SSO2049"/>
    <dbReference type="DNASU" id="1453563"/>
    <dbReference type="EnsemblBacteria" id="AAK42236">
        <property type="protein sequence ID" value="AAK42236"/>
        <property type="gene ID" value="SSO2049"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2049"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2131"/>
    <dbReference type="eggNOG" id="arCOG06046">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131319_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WR6"/>
    <dbReference type="PhylomeDB" id="Q97WR6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="19521" checksum="F00C31A9951A5A55" modified="2001-10-01" version="1">MRISLLSLHFSLFNKRFFSNPNINENMDLIELLKFEHGIFRIRFYFLEKVDNSLQELETLHDFIVNVHAKMEDLYVFKDIPEAKPYSNDHKLIEKYGDTIIKEKRKDWVPRYMKIVLDHNLNEEKYVFPKVKERKGLVLDIIEQYGFENYQKITGIDIRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WU3</accession>
    <name>Q97WU3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl-CoA synthetase (Acetate-CoA ligase) carboxy-end (AcsA-4)</fullName>
            <ecNumber evidence="4">6.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">acsA-4</name>
        <name evidence="4" type="ordered locus">SSO2021</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.1" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90368">
        <property type="entry name" value="H90368"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WU3"/>
    <dbReference type="FunCoup" id="Q97WU3">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2021"/>
    <dbReference type="PaxDb" id="273057-SSO2021"/>
    <dbReference type="EnsemblBacteria" id="AAK42207">
        <property type="protein sequence ID" value="AAK42207"/>
        <property type="gene ID" value="SSO2021"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2021"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2097"/>
    <dbReference type="eggNOG" id="arCOG06122">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_17_0_2"/>
    <dbReference type="InParanoid" id="Q97WU3"/>
    <dbReference type="PhylomeDB" id="Q97WU3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016878">
        <property type="term" value="F:acid-thiol ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43352">
        <property type="entry name" value="ACETYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43352:SF1">
        <property type="entry name" value="ANTHRANILATE--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="3">
        <location>
            <begin position="177"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="267" mass="30431" checksum="4C56FEFFBAD5F719" modified="2001-10-01" version="1">METIHKYKPTIFFGVPTLYNAMLHVEDWKNYNLTSLRFCVSAGEPLPPLIYIKWKERYGVEILDGIGSTEALHIYISNIPSNSKPGCTGKPVPGYEVKIVDEDGNEVPPKTIGDLYVKGDSVAMFYWNDYESTRKNMMGSWFRTGDKFYRDDEGYYYYVGRSDDMVKTAGLWVSPIEVESALLSHPAVLEAAVVGIPDEVGLVRVVGFVTLKQGYSPSSELAEEIRNYLREKLDHYKVPKEIRFITEIPKTATGKIQRYKFRLGEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WU4</accession>
    <name>Q97WU4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acetyl-CoA synthetase (Acetate-CoA ligase) amino-end (AcsA-4)</fullName>
            <ecNumber evidence="3">6.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">acsA-4</name>
        <name evidence="3" type="ordered locus">SSO2020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.1" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90368">
        <property type="entry name" value="G90368"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WU4"/>
    <dbReference type="STRING" id="273057.SSO2020"/>
    <dbReference type="PaxDb" id="273057-SSO2020"/>
    <dbReference type="EnsemblBacteria" id="AAK42206">
        <property type="protein sequence ID" value="AAK42206"/>
        <property type="gene ID" value="SSO2020"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2020"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2096"/>
    <dbReference type="eggNOG" id="arCOG06122">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_965118_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WU4"/>
    <dbReference type="PhylomeDB" id="Q97WU4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016878">
        <property type="term" value="F:acid-thiol ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000140">
        <property type="entry name" value="Acetyl-CoA synthetase (Acetate-CoA ligase) amino-end (AcsA-4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43352">
        <property type="entry name" value="ACETYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43352:SF1">
        <property type="entry name" value="ANTHRANILATE--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="26"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="288" mass="32816" checksum="F4144937A712C2B9" modified="2001-10-01" version="1">MTAKYGRILNVTDALFSKWEDSEADKRIAIYYKDEVWSYRKVIEEINRVGNALRELGVEKENRILIIAYDTPFFISIFYSAMKIGAIPVPVNTYLKPEEYLFLLEDTGAKVLAIEPDIWPKLSPYLKGRADKLKHVIILPSISETQKITFEDHNHKAELSFYHGLVSTASTHLEAEKTSPNDMAFWLYTSGTTGHPKAAVHLHKDILVVLDTYVKNVLKISEKDRLFSASKLFFAYGLGNGSYFAFGNGASTVLMPDRVEPEGYWRPYINTSLLSSLECLLYITQCYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X21</accession>
    <name>Q97X21_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1931</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90358">
        <property type="entry name" value="H90358"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X21"/>
    <dbReference type="STRING" id="273057.SSO1931"/>
    <dbReference type="PaxDb" id="273057-SSO1931"/>
    <dbReference type="EnsemblBacteria" id="AAK42127">
        <property type="protein sequence ID" value="AAK42127"/>
        <property type="gene ID" value="SSO1931"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1931"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2006"/>
    <dbReference type="eggNOG" id="arCOG08449">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1718299_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X21"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="152" mass="17776" checksum="10231E2DE8CD1EA4" modified="2001-10-01" version="1">MSEEKMSEESPFDKIMHMPLDKVREILNRTNLERQVFNEIVGDNWKVKIIETKVNKNNYETIYRLYGIYLGDKSVAVSVTSDGKLRHIILNDVKVVSETDQTVKKKNTMLLLDYENQRAIFTEGEMELWKGKIGFDAIKSINILKNESRDFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X59</accession>
    <name>Q97X59_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90353">
        <property type="entry name" value="F90353"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992765.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X59"/>
    <dbReference type="STRING" id="273057.SSO1894"/>
    <dbReference type="PaxDb" id="273057-SSO1894"/>
    <dbReference type="DNASU" id="1453412"/>
    <dbReference type="EnsemblBacteria" id="AAK42085">
        <property type="protein sequence ID" value="AAK42085"/>
        <property type="gene ID" value="SSO1894"/>
    </dbReference>
    <dbReference type="GeneID" id="1453412"/>
    <dbReference type="KEGG" id="sso:SSO1894"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1950"/>
    <dbReference type="eggNOG" id="arCOG06009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1163847_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X59"/>
    <dbReference type="PhylomeDB" id="Q97X59"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="241" mass="26241" checksum="6F009396B4930554" modified="2001-10-01" version="1">MTFNGIKFDTSVPGMLPWEIVVIYFIIGLAILFYFAKRFGLKSFTTIDLVYIAVGAAFSVVWEFYIGSFIGRFLPSTPFIGVGFWGRMFILLIVAALVRKPGTGILSLLIFNILSDLFFYGFGGEPMYTIYEALTYGLFLDLVIIGSRGKLFGIGYKSADGSSVATRTTVGLAIVEGIIIGILFAIPDPIFYLGFFKPLISGAIVNWATIQFDFLAFIPGDVIIGILGALAGQRIAKAVGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X78</accession>
    <name>Q97X78_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH asnC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1876</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90351">
        <property type="entry name" value="A90351"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923635.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X78"/>
    <dbReference type="STRING" id="273057.SSO1876"/>
    <dbReference type="PaxDb" id="273057-SSO1876"/>
    <dbReference type="EnsemblBacteria" id="AAK42064">
        <property type="protein sequence ID" value="AAK42064"/>
        <property type="gene ID" value="SSO1876"/>
    </dbReference>
    <dbReference type="GeneID" id="1453391"/>
    <dbReference type="KEGG" id="sso:SSO1876"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1927"/>
    <dbReference type="eggNOG" id="arCOG01586">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090242_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X78"/>
    <dbReference type="PhylomeDB" id="Q97X78"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF8">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR PTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="256" mass="29881" checksum="6CD5E348F34FFC22" modified="2001-10-01" version="1">MDIIDKRILFYLLKDGRISQRRIASLLNLTPASLNYRFKKLTDSGILKGFKLYVNPNFFGKYQIFIAFKNYSDIDADWISFKLKCLEWLNVYGIYASDNTELKDKISYMTKNLGDPVLSYFPVQSLFRASNLDQKIVEILKIDPRLSSSEISKKINVNSRIIEKHIRYLRHRGLILVAPQIDLGKTDIIIFSIFSKNIDEISVVLQECKLWQFTDGYAGITVCYADNMERARKYIDLARQIDNTADVMIIYDYMFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X91</accession>
    <name>Q97X91_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Microsomal dipeptidase</fullName>
            <ecNumber evidence="1">3.4.13.19</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1864</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.13.19" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90349">
        <property type="entry name" value="D90349"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X91"/>
    <dbReference type="FunCoup" id="Q97X91">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1864"/>
    <dbReference type="PaxDb" id="273057-SSO1864"/>
    <dbReference type="EnsemblBacteria" id="AAK42051">
        <property type="protein sequence ID" value="AAK42051"/>
        <property type="gene ID" value="SSO1864"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1864"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1913"/>
    <dbReference type="eggNOG" id="arCOG04083">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031404_2_1_2"/>
    <dbReference type="InParanoid" id="Q97X91"/>
    <dbReference type="PhylomeDB" id="Q97X91"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016805">
        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070573">
        <property type="term" value="F:metallodipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01301">
        <property type="entry name" value="rDP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008257">
        <property type="entry name" value="Pept_M19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443:SF12">
        <property type="entry name" value="DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443">
        <property type="entry name" value="MICROSOMAL DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01244">
        <property type="entry name" value="Peptidase_M19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51365">
        <property type="entry name" value="RENAL_DIPEPTIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="314" mass="35455" checksum="D913FAA3BB451FC8" modified="2001-10-01" version="1">MEVKMKLIDLHEDLAYSNQQGIDVIEGDRQSSIRMLKEFDSLVFASIFPHVDTLDERSEGLTALYGNPTRSTNFSIELFLDQVKFYYYLERKNLVKIVRSANDLEKEGVKLLLSLEGADVLRDYSDIYLLKELHVLNLGLTWNYDNKFASSCMSKKDYGLTSEGEELVRLANKLGIIIDLAHAGKRTVLDVTSTSKKPVIDSHTNFTRLKQHRRNLDDEEIEAIVKTKGVIGITAIVATLKEASISGIVESIKYLGESFGWDYVAIGTDFLGIGEAPKGFENILKVKELTKAIEGHEEEVLWKNAFRVIKENIS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-05" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X94</accession>
    <name>UPPP_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Undecaprenyl-diphosphatase</fullName>
            <ecNumber evidence="1">3.6.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl pyrophosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uppP</name>
        <name type="synonym">bacA</name>
        <name type="synonym">upk</name>
        <name type="ordered locus">SSO1860</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>di-trans,octa-cis-undecaprenyl diphosphate + H2O = di-trans,octa-cis-undecaprenyl phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28094"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="EC" id="3.6.1.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UppP family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.27" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99349">
        <property type="entry name" value="A99349"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988521.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X94"/>
    <dbReference type="SMR" id="Q97X94"/>
    <dbReference type="STRING" id="273057.SSO1860"/>
    <dbReference type="PaxDb" id="273057-SSO1860"/>
    <dbReference type="EnsemblBacteria" id="AAK42048">
        <property type="protein sequence ID" value="AAK42048"/>
        <property type="gene ID" value="SSO1860"/>
    </dbReference>
    <dbReference type="GeneID" id="1453375"/>
    <dbReference type="KEGG" id="sso:SSO1860"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1910"/>
    <dbReference type="eggNOG" id="arCOG04761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060296_1_2_2"/>
    <dbReference type="InParanoid" id="Q97X94"/>
    <dbReference type="PhylomeDB" id="Q97X94"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050380">
        <property type="term" value="F:undecaprenyl-diphosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000270">
        <property type="term" value="P:peptidoglycan metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01006">
        <property type="entry name" value="Undec_diphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003824">
        <property type="entry name" value="UppP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001398">
        <property type="entry name" value="PRK00281.3-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30622">
        <property type="entry name" value="UNDECAPRENYL-DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30622:SF2">
        <property type="entry name" value="UNDECAPRENYL-DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02673">
        <property type="entry name" value="BacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000151254" description="Undecaprenyl-diphosphatase">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01006"/>
        </source>
    </evidence>
    <sequence length="278" mass="29967" checksum="922897405C584460" modified="2001-10-01" version="1">MNYILIGVILGIVQGISEWIPISSKTQVLIVSSTLLGLSFNVAYSFGLFMEIGTIAAAIFYFRSEISGLLKALVRMSSRREDYLLLKFLVIVTIITGLVGVPLYLFVISLPILGLPMTVLGVVLLIDGIVIYLSRKNYFPRKGLHDLKLRDIIIVGIAQGLAALPGVSRSGMTTSALILLGVKPEEAFKLSFISLIPAALGAISVTVLFSKHEVSQAVHSVSLSGLLISIVVATFVSIFFINALLRFARTNKVVLLVIILGIMAIISGILSDIAKGFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XJ2</accession>
    <name>Q97XJ2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1058</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90335">
        <property type="entry name" value="E90335"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XJ2"/>
    <dbReference type="PaxDb" id="273057-SSO1744"/>
    <dbReference type="EnsemblBacteria" id="AAK41940">
        <property type="protein sequence ID" value="AAK41940"/>
        <property type="gene ID" value="SSO1744"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1744"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1788"/>
    <dbReference type="eggNOG" id="arCOG02754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1551894_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XJ2"/>
    <dbReference type="PhylomeDB" id="Q97XJ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053172">
        <property type="entry name" value="Tn903_transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631:SF3">
        <property type="entry name" value="ISSOD12 TRANSPOSASE TNPA_ISSOD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="29"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="184" mass="21219" checksum="9E8D570FEE2E7128" modified="2001-10-01" version="1">MKIIPTSLDYSTIWERVRNMNITFPEASDELEVIADATGISTNTGGQYIVAKWGKTRDSKFLKIEIVMDNNEFNVINAEVTSNEVESAVKTVKDLQDKGKKFYEDKTYDANEVYKTGVEVVVPPKKNASTKRHLARRKAVREFRKLGYDRWREEKGYGVRWRVESLFSVKRTFGESVRQVLLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XJ7</accession>
    <name>Q97XJ7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase AAA-type core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1739</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90334">
        <property type="entry name" value="H90334"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990359.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XJ7"/>
    <dbReference type="PaxDb" id="273057-SSO1739"/>
    <dbReference type="EnsemblBacteria" id="AAK41935">
        <property type="protein sequence ID" value="AAK41935"/>
        <property type="gene ID" value="SSO1739"/>
    </dbReference>
    <dbReference type="GeneID" id="1454714"/>
    <dbReference type="KEGG" id="sso:SSO1739"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1781"/>
    <dbReference type="eggNOG" id="arCOG03239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_881721_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XJ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051396">
        <property type="entry name" value="Bact_Antivir_Def_Nuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43581">
        <property type="entry name" value="ATP/GTP PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43581:SF4">
        <property type="entry name" value="ATP_GTP PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13304">
        <property type="entry name" value="AAA_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase AAA-type core" evidence="1">
        <location>
            <begin position="22"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="315" mass="35922" checksum="B2AFF1A887951363" modified="2001-10-01" version="1">MIENISIGNFRGLKLNVNLGRINAIIGRNGTGKTSFLEALFFSSLFLSDYSESDISSLMIYAFNSRGDMISAFLSLTDSEVELGLHGGERIKLSFRRKEKEKIDIYLLKDESKKDEKIASISLSSGILAKETISVPIRVPEVKLIKRHKIMEHFLPVYLSVYFDFYDYPERIIGSAKKKGVKSDFEILPDEMGLYKVHYVKENETKPAYVIGRGLLKKELIIDSLTYSNLLLIDEIENSLHPDLLLEIFNVMKDKGKNSQIVFTTHSNEVIKMMTKVFDDSNAKIIYLSTRGTSREYKLSEISEIDEPLSWVGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XK3</accession>
    <name>Q97XK3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90334">
        <property type="entry name" value="B90334"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XK3"/>
    <dbReference type="STRING" id="273057.SSO1730"/>
    <dbReference type="PaxDb" id="273057-SSO1730"/>
    <dbReference type="EnsemblBacteria" id="AAK41929">
        <property type="protein sequence ID" value="AAK41929"/>
        <property type="gene ID" value="SSO1730"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1730"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1773"/>
    <dbReference type="eggNOG" id="arCOG02665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081189_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XK3"/>
    <dbReference type="PhylomeDB" id="Q97XK3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09656">
        <property type="entry name" value="Cmr3_III-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010165">
        <property type="entry name" value="CRISPR-Cmr3_IIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052875">
        <property type="entry name" value="CRISPR_assoc_ribonuclease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01888">
        <property type="entry name" value="cas_cmr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37169:SF2">
        <property type="entry name" value="CRISPR SYSTEM CMR SUBUNIT CMR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37169">
        <property type="entry name" value="CRISPR SYSTEM ENDORIBONUCLEASE CSX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="314" mass="35282" checksum="97802D72A0C35F47" modified="2001-10-01" version="1">MIKMRIKPLEEYRIGLRQSSGIVIENVDVISAFPSPTTILGIIGAMKNAKPSQLSGIDDLREAYTSLTGNNTLSGDCDSSDNPLIWGPLIKEKDNKIYTPLFFNKLILDPIEYVKTSLNENVKVQSESLVKEIGIQIRRMNQMNRGSRNTIHLFYQKFFGSNYELEYCINIDDLKKLVKENVVALGGENRYARVRIDNGNCNFGNGNYAILLQPLLFESDSEYFVRLDNVKGLGCIDEIYGVLVEKGNKIDFNNFKVKVVYYALGYGNERRPMLQALPPGTVIKVKETCNNAKALGLLSELGFGAIYRVDPGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XN0</accession>
    <name>Q97XN0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Third ORF in transposon ISC1173</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO8684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90329">
        <property type="entry name" value="F90329"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XN0"/>
    <dbReference type="STRING" id="273057.SSO8684"/>
    <dbReference type="PaxDb" id="273057-SSO8684"/>
    <dbReference type="DNASU" id="1454672"/>
    <dbReference type="EnsemblBacteria" id="AAK41893">
        <property type="protein sequence ID" value="AAK41893"/>
        <property type="gene ID" value="SSO8684"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8684"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1733"/>
    <dbReference type="eggNOG" id="arCOG02133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076276_1_3_2"/>
    <dbReference type="InParanoid" id="Q97XN0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051354">
        <property type="entry name" value="Transposase_27_IS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293:SF1">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="84" mass="9833" checksum="ACCC2AC76AB121AE" modified="2001-10-01" version="1">MRKVGIRRRESRPTCPSCNSNRVVKNGSSRGKAKYKCKACGRTFYDALKHRMDREQRKRILKEYLNRMSMRAISRVEGKPLNTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XN1</accession>
    <name>Q97XN1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1683</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90329">
        <property type="entry name" value="E90329"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XN1"/>
    <dbReference type="STRING" id="273057.SSO1683"/>
    <dbReference type="PaxDb" id="273057-SSO1683"/>
    <dbReference type="EnsemblBacteria" id="AAK41892">
        <property type="protein sequence ID" value="AAK41892"/>
        <property type="gene ID" value="SSO1683"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1683"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1732"/>
    <dbReference type="eggNOG" id="arCOG02133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135438_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XN1"/>
    <dbReference type="PhylomeDB" id="Q97XN1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051354">
        <property type="entry name" value="Transposase_27_IS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293:SF1">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="167" mass="20026" checksum="5C7C384F2FB5C46F" modified="2001-10-01" version="1">MRRIEIRAFTYLTILKGELKNFTAKSTVFDEFWTYFRVRHGVMREDLWIWTADGVPFYEFGDRSYGTFRLGWLPRSEVNYTDYYCVYQVLDNRVAGKKYTYLVESHNSWCRLARLARDTKAVTRSMRMTEYSLALLNVMYPHVFSREGTPLNETYLKGVQYIRENLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XQ4</accession>
    <name>Q97XQ4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1656</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90326">
        <property type="entry name" value="F90326"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991009.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XQ4"/>
    <dbReference type="STRING" id="273057.SSO1656"/>
    <dbReference type="PaxDb" id="273057-SSO1656"/>
    <dbReference type="EnsemblBacteria" id="AAK41869">
        <property type="protein sequence ID" value="AAK41869"/>
        <property type="gene ID" value="SSO1656"/>
    </dbReference>
    <dbReference type="GeneID" id="1454648"/>
    <dbReference type="KEGG" id="sso:SSO1656"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1705"/>
    <dbReference type="eggNOG" id="arCOG03431">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1269983_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XQ4"/>
    <dbReference type="PhylomeDB" id="Q97XQ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38133">
        <property type="entry name" value="SLR1429 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38133:SF1">
        <property type="entry name" value="SLR1429 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="217" mass="25382" checksum="6B5F0D4D8E9139AA" modified="2001-10-01" version="1">MKRGWLFDFGKNIREEIDDGRASRGREYVRNGAVRKIEINPGKIAAQVSGTKLYDVNIYGNTLSEEDKRKVINIINKKGYLSSLLSYKFPTSIIDDLDEEGIELIPRNLSYECTCPDYQRPCKHIYAVYLTAYDIISNNPLLLFKFLGINKEELIYEKGQKIMQEISVKDFQRKYEFPSIKFVNAKPWNKFGNDKEVVKHMYMLIMKTAERKIDELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XR3</accession>
    <name>Q97XR3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="4">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mdlC</name>
        <name evidence="11" type="ordered locus">SSO1647</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90325">
        <property type="entry name" value="E90325"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923544.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XR3"/>
    <dbReference type="SMR" id="Q97XR3"/>
    <dbReference type="STRING" id="273057.SSO1647"/>
    <dbReference type="PaxDb" id="273057-SSO1647"/>
    <dbReference type="EnsemblBacteria" id="AAK41860">
        <property type="protein sequence ID" value="AAK41860"/>
        <property type="gene ID" value="SSO1647"/>
    </dbReference>
    <dbReference type="GeneID" id="1454639"/>
    <dbReference type="KEGG" id="sso:SSO1647"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1695"/>
    <dbReference type="eggNOG" id="arCOG03656">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_1_2"/>
    <dbReference type="InParanoid" id="Q97XR3"/>
    <dbReference type="PhylomeDB" id="Q97XR3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02002">
        <property type="entry name" value="TPP_BFDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="10">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="8">
        <location>
            <begin position="188"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="9">
        <location>
            <begin position="385"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK41860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="503" mass="56980" checksum="7E861EB16BB1D2BA" modified="2001-10-01" version="1">MKVGNALFKLMSELGIRQVFGNPGTTELSFLKHMPKDFNYYLALHDGISVGMAEGYYFATRKPQIVNLHSSPGLTNAMGFIYEALISRIPLIVLVGQQYLYRLIDEPVLYGDFIKISQGVVKSAYEIRSEKDAIKTFIRAYKESITPPYGPVLISLPQDIPDMEVSEGEKVDIPKYFVSGTCDTSAIEFVLDKVKSASSIAIVAGYEVSIFSAHEELVRLAEKLNAPIYTEPYLSIFPIDSSNILFKGPLSRYKASDVVKELEKYDLVLVIGGWLNYVVFPDVDIRLNIVEVTSDFKEASKRKWDTIVCNPKDFLIKLYNMLYKGLNKNIIKRENRDLELQGDFITEVFKEMKGYLDKYTIFAEIPTYRDTLIKIIELKPSSLYITRSGLLGWALSALVGYSINGAKVLAIIGDGSFNYTPQALWSAVKYSTRLKVIVINNEGYASLSRHGVEADWLFPSTSPWKVALAYGFEAKESRDIKNDLKWLFEDDKRKLLEIRLARH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XS6</accession>
    <name>Q97XS6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1634</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90323">
        <property type="entry name" value="G90323"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923533.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XS6"/>
    <dbReference type="STRING" id="273057.SSO1634"/>
    <dbReference type="PaxDb" id="273057-SSO1634"/>
    <dbReference type="EnsemblBacteria" id="AAK41846">
        <property type="protein sequence ID" value="AAK41846"/>
        <property type="gene ID" value="SSO1634"/>
    </dbReference>
    <dbReference type="GeneID" id="84058441"/>
    <dbReference type="KEGG" id="sso:SSO1634"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1675"/>
    <dbReference type="eggNOG" id="arCOG05401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130233_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XS6"/>
    <dbReference type="PhylomeDB" id="Q97XS6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="162" mass="18211" checksum="FD84838AFD3E6CA8" modified="2001-10-01" version="1">MSQENNENKKKTITIRGVDEKIYRKLTEIARSSGKTIGEVTNQAFRTFLDLSDAAKRTASGVVESGKTFIEGFKEGIGNLIIISDIDELSVNKEEMMQTSKPIAFKGIKKLSLVNISDDDVEKYIQEISDVDELIIPPNINKLKLLQKCRRVKKIIVQNNPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XZ5</accession>
    <name>Q97XZ5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4143 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1563</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99315">
        <property type="entry name" value="A99315"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XZ5"/>
    <dbReference type="PaxDb" id="273057-SSO1563"/>
    <dbReference type="EnsemblBacteria" id="AAK41776">
        <property type="protein sequence ID" value="AAK41776"/>
        <property type="gene ID" value="SSO1563"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1563"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1596"/>
    <dbReference type="eggNOG" id="arCOG03167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1801786_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XZ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295:SF18">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="17081" checksum="0E0E11DBBC36E429" modified="2001-10-01" version="1">MEKLFIIRTFRKREKGGKTNYRSLKKVYFIDPFFFRVMKIYSVGKDVDDNEIPLIIEGIVGEHLAREYKEVGYLHFKSGKEVDFSVGNVKVEVKWREKERKRYNNVNYVLTIDEFKKSDEQLTLPVSIFLYLISSNKTFYELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y35</accession>
    <name>Q97Y35_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PaREP1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90310">
        <property type="entry name" value="A90310"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990105.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y35"/>
    <dbReference type="PaxDb" id="273057-SSO1515"/>
    <dbReference type="DNASU" id="1454515"/>
    <dbReference type="EnsemblBacteria" id="AAK41736">
        <property type="protein sequence ID" value="AAK41736"/>
        <property type="gene ID" value="SSO1515"/>
    </dbReference>
    <dbReference type="GeneID" id="1454515"/>
    <dbReference type="KEGG" id="sso:SSO1515"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1554"/>
    <dbReference type="eggNOG" id="arCOG03721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115256_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y35"/>
    <dbReference type="PhylomeDB" id="Q97Y35"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="18043" checksum="B93AB12D6A49C75D" modified="2001-10-01" version="1">MEELIKKAKEYDIDINDLIIDAISRKDPKEAINLRIELAKKYITETEDYLKKGDAVQASEKAYKTAEEIVKALAEKFNIPEYQQALKEGRWYTYWLASAVNRLAKDLGDWVLNGWNSAYILHVWGFHEAKLSTADISEHLRKVKEMLDNVINIIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y53</accession>
    <name>Q97Y53_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1493</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90307">
        <property type="entry name" value="G90307"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y53"/>
    <dbReference type="STRING" id="273057.SSO1493"/>
    <dbReference type="PaxDb" id="273057-SSO1493"/>
    <dbReference type="EnsemblBacteria" id="AAK41718">
        <property type="protein sequence ID" value="AAK41718"/>
        <property type="gene ID" value="SSO1493"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1493"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1532"/>
    <dbReference type="eggNOG" id="arCOG00726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121774_2_0_2"/>
    <dbReference type="InParanoid" id="Q97Y53"/>
    <dbReference type="PhylomeDB" id="Q97Y53"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09873">
        <property type="entry name" value="PIN_Pae0151-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044153">
        <property type="entry name" value="PIN_Pae0151-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051619">
        <property type="entry name" value="TypeII_TA_RNase_PINc/VapC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901:SF1">
        <property type="entry name" value="EXONUCLEASE VAPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901">
        <property type="entry name" value="RIBONUCLEASE VAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="2">
        <location>
            <begin position="8"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="107" mass="11934" checksum="7207B351E3EFFCD0" modified="2001-10-01" version="1">MKDAETLDFALVEVSNVVWKKAVLTGELTGKDVIKAITIVKEYLPQLLTVNKSIDLIERAIEISVNEKITVYDSLYIALAECKGSKLVTGDKKQYDVAKKYVISELI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y91</accession>
    <name>CSA2B_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated aCascade subunit Cas7/Csa2 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>CRISPR-associated aCascade subunit Cas7/Csa2, subtype I-A/Apern 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">csa2b</name>
        <name type="synonym">cas7b</name>
        <name type="ordered locus">SSO1442</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="J. Biol. Chem." volume="286" first="21643" last="21656">
            <title>Structural and functional characterization of an archaeal clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for antiviral defense (CASCADE).</title>
            <authorList>
                <person name="Lintner N.G."/>
                <person name="Kerou M."/>
                <person name="Brumfield S.K."/>
                <person name="Graham S."/>
                <person name="Liu H."/>
                <person name="Naismith J.H."/>
                <person name="Sdano M."/>
                <person name="Peng N."/>
                <person name="She Q."/>
                <person name="Copie V."/>
                <person name="Young M.J."/>
                <person name="White M.F."/>
                <person name="Lawrence C.M."/>
            </authorList>
            <dbReference type="PubMed" id="21507944"/>
            <dbReference type="DOI" id="10.1074/jbc.M111.238485"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
        <scope>RNA-BINDING</scope>
        <scope>INTERACTION WITH CAS5E</scope>
        <scope>MUTAGENESIS OF HIS-160</scope>
        <source>
            <strain>ATCC 35091 / DSM 1616 / JCM 8930 / NBRC 15331 / P1</strain>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the aCascade ribonucleoprotein complex, minimally composed of Csa2 and Cas5a, which binds crRNA. Other possible components of aCascade in strain P1 are Cas6b (SSO1437) and Csa5 (SSO1443), while SSO1399, Cas5b (SSO1400) and SSO1401 have sometimes been seen weakly associated. Csa2 is probably the major RNA-binding subunit. The Csa2-Cas5a-crRNA complex also binds target DNA homologous to crRNA, probably forming an R-loop. Purified aCascade forms a filament about 6 nm in width.</text>
    </comment>
    <comment type="miscellaneous">
        <text>Crystallography and cloning were done with strain P2, while interaction experiments were done with genes cloned from strain P2 but over-expressed in P1. The aCascade complex was purified from strain P1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR-associated protein Cas7/Cst2/DevR family. Subtype I-a/Apern subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="AAK41675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="D90302"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B/C/D=1-321"/>
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        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
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        <property type="resolution" value="3.50 A"/>
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        <property type="resolution" value="3.20 A"/>
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        <property type="method" value="EM"/>
        <property type="resolution" value="3.07 A"/>
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    <dbReference type="PDBsum" id="3PS0"/>
    <dbReference type="PDBsum" id="9CP1"/>
    <dbReference type="PDBsum" id="9CP2"/>
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    <dbReference type="PDBsum" id="9CRO"/>
    <dbReference type="PDBsum" id="9CRP"/>
    <dbReference type="PDBsum" id="9CRQ"/>
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    <dbReference type="EMDB" id="EMD-45796"/>
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    <dbReference type="EnsemblBacteria" id="AAK41675">
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        <property type="gene ID" value="SSO1442"/>
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    <dbReference type="PATRIC" id="fig|273057.12.peg.1471"/>
    <dbReference type="eggNOG" id="arCOG03617">
        <property type="taxonomic scope" value="Archaea"/>
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        <property type="component" value="Chromosome"/>
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        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:defense response to virus"/>
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    </dbReference>
    <dbReference type="CDD" id="cd09685">
        <property type="entry name" value="Cas7_I-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002764">
        <property type="entry name" value="Cas7/Cst2/DevR_sub_I-a/Apern"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010154">
        <property type="entry name" value="CRISPR-assoc_Cas7/Cst2/DevR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052681">
        <property type="entry name" value="CRISPR-Cas7/Cst2/DevR"/>
    </dbReference>
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        <property type="entry name" value="cas_MJ0381"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DevR_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37459">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37459:SF1">
        <property type="entry name" value="CRISPR-ASSOCIATED PROTEIN CAS7_CST2_DEVR"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01905">
        <property type="entry name" value="DevR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000417885" description="CRISPR-associated aCascade subunit Cas7/Csa2 2">
        <location>
            <begin position="1"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Significantly reduced affinity for crRNA." evidence="2">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="12"/>
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        </location>
    </feature>
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        <location>
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    </feature>
    <feature type="strand" evidence="4">
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            <begin position="28"/>
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        </location>
    </feature>
    <feature type="strand" evidence="4">
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="helix" evidence="4">
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        </location>
    </feature>
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        <location>
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        </location>
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    <feature type="helix" evidence="4">
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="116"/>
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        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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    <feature type="helix" evidence="4">
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    <feature type="helix" evidence="4">
        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="21507944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3PS0"/>
        </source>
    </evidence>
    <sequence length="321" mass="35265" checksum="0B5959530AC2709A" modified="2001-10-01" version="1">MISGSVRFLVNLESLNGVESIGNLTKHRTAPVVLKTSTGYLVRYVPVISGEALAHAYQASLVDIAKKEGLPVGSLSSQYEFIKFSTDEALKIEGIKEPKDYNDARRFEVEVMLKDVIADVGGFMYAGGAPVRRTSRIKLGYMIPALRGDEIPAQLEAQFHVRFSNKPVSGSQAIFNVEVSSALYTFSFELDEDLIAVPSTFGEKVKGEEELERQKAKRVKSAIKALYSLLSGNFGGKRSRFLPSMKLMSLVVTKTDFPFMPEPAHDDDYIKTTIMRLGKAKGVLNGNLAKAYVINNEGIEVGEGVTVLSTVEDLVVKLEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-01-24" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YB7</accession>
    <name>Q97YB7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1412</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
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            <taxon>Sulfolobaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
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            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <source>
            <dbReference type="EMBL" id="AAK41646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YD0</accession>
    <name>Q97YD0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposase ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1396</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
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        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="H90296"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YD0"/>
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    <dbReference type="EnsemblBacteria" id="AAK41631">
        <property type="protein sequence ID" value="AAK41631"/>
        <property type="gene ID" value="SSO1396"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1396"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1412"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074024_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025471">
        <property type="entry name" value="DUF4322"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033541">
        <property type="entry name" value="transpos_ISH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14210">
        <property type="entry name" value="DUF4322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4322" evidence="3">
        <location>
            <begin position="14"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="108"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="364" mass="42070" checksum="4BE65E9F0E78C539" modified="2001-10-01" version="1">MRKPVLSRGEDKVITPGLPHQNNIQQVGYKLLSMLSFKGRKAEEVSRVLVSACLWNDSVESKSKGYNVSPQTVRNYVEEQGTEVIEKLLESMRRISMEILKGVKEVDISIDWTTKTWYGKPVEGLGSSAKGNSWNYATATTKYQNMVLLLAFVPQVNGMSKDEIVKLLMEQIVGMGLKVGLVTLDAGFYTVEVLKFISQFKFVIGVPVGDVKIYEEFDGEYTTNSKRYKKEEQVKFRLLVYGKEIVKKRKKTVVYFARATNLDLPKREVLKLYNKVRSPIETSYRNIKAFLPFTSSTKFIFRELIFVLAMIFYSLYTVFKNVMTREEFRLLLILCFLDDLSDLKDFIFNLEETLINTIDLFLWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YE7</accession>
    <name>Q97YE7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1379</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90294">
        <property type="entry name" value="F90294"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992590.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YE7"/>
    <dbReference type="STRING" id="273057.SSO1379"/>
    <dbReference type="PaxDb" id="273057-SSO1379"/>
    <dbReference type="EnsemblBacteria" id="AAK41613">
        <property type="protein sequence ID" value="AAK41613"/>
        <property type="gene ID" value="SSO1379"/>
    </dbReference>
    <dbReference type="GeneID" id="1454392"/>
    <dbReference type="KEGG" id="sso:SSO1379"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1384"/>
    <dbReference type="HOGENOM" id="CLU_341208_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YE7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="730"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="756"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="792"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="831" mass="95972" checksum="AF1107E052D59DE4" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YI6</accession>
    <name>Q97YI6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">First ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1335</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90289">
        <property type="entry name" value="F90289"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923330.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YI6"/>
    <dbReference type="STRING" id="273057.SSO1335"/>
    <dbReference type="PaxDb" id="273057-SSO1335"/>
    <dbReference type="EnsemblBacteria" id="AAK41573">
        <property type="protein sequence ID" value="AAK41573"/>
        <property type="gene ID" value="SSO1335"/>
    </dbReference>
    <dbReference type="GeneID" id="1454352"/>
    <dbReference type="KEGG" id="sso:SSO1335"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1341"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="InParanoid" id="Q97YI6"/>
    <dbReference type="PhylomeDB" id="Q97YI6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04762">
        <property type="entry name" value="HTH_MerR-trunc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2170:FF:000002">
        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1390:FF:000002">
        <property type="entry name" value="ORF1 in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="4">
        <location>
            <begin position="55"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="192" mass="22343" checksum="DDBED46EC772F202" modified="2001-10-01" version="1">MLRSKEACQRLGISYATLREYVKKGYIKPVILQSGKWRFREEDVERLMGIIRKRKVILYARVSSSTQKDDLANQVKYLEEQVKEYDQVITDIGSGLNMKRKGFLKLLRMILNNEVSRVVIAYPDRLVRFGFEILEEVCKAHGCEIVVINQEDKEEELVEDLVSILLSFSGKLHGMRSHKYEKVKKCAEELKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YK4</accession>
    <name>Q97YK4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Second ORF in transposon ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO7675</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90287">
        <property type="entry name" value="D90287"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YK4"/>
    <dbReference type="STRING" id="273057.SSO7675"/>
    <dbReference type="PaxDb" id="273057-SSO7675"/>
    <dbReference type="DNASU" id="1454334"/>
    <dbReference type="EnsemblBacteria" id="AAK41555">
        <property type="protein sequence ID" value="AAK41555"/>
        <property type="gene ID" value="SSO7675"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7675"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2695947_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YK4"/>
    <dbReference type="PhylomeDB" id="Q97YK4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="2"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="73" mass="8325" checksum="B9D130368E937127" modified="2001-10-01" version="1">MTCARCGYVREDLTLFDRVFVCPKCGWIVDRDYNASLNILRRSGSERPSVWSSAFYRWQGGAMKQEALSVRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YK9</accession>
    <name>Q97YK9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl-CoA synthetase (Acetate-CoA ligase) (AcsA-1)</fullName>
            <ecNumber evidence="4">6.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">acsA-1</name>
        <name evidence="4" type="ordered locus">SSO1314</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.1" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90286">
        <property type="entry name" value="G90286"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923307.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YK9"/>
    <dbReference type="FunCoup" id="Q97YK9">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1314"/>
    <dbReference type="PaxDb" id="273057-SSO1314"/>
    <dbReference type="EnsemblBacteria" id="AAK41550">
        <property type="protein sequence ID" value="AAK41550"/>
        <property type="gene ID" value="SSO1314"/>
    </dbReference>
    <dbReference type="GeneID" id="1454329"/>
    <dbReference type="KEGG" id="sso:SSO1314"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1315"/>
    <dbReference type="eggNOG" id="arCOG06122">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_10_2"/>
    <dbReference type="InParanoid" id="Q97YK9"/>
    <dbReference type="PhylomeDB" id="Q97YK9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016878">
        <property type="term" value="F:acid-thiol ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000048">
        <property type="entry name" value="Coenzyme A ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011957">
        <property type="entry name" value="Benz_CoA_lig"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02262">
        <property type="entry name" value="benz_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43352">
        <property type="entry name" value="ACETYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43352:SF1">
        <property type="entry name" value="ANTHRANILATE--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="25"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="3">
        <location>
            <begin position="439"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="529" mass="60554" checksum="636901ED7E0FD7AA" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YL6</accession>
    <name>Q97YL6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Magnesium transporter CorA family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1307</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90285">
        <property type="entry name" value="H90285"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923300.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YL6"/>
    <dbReference type="STRING" id="273057.SSO1307"/>
    <dbReference type="PaxDb" id="273057-SSO1307"/>
    <dbReference type="EnsemblBacteria" id="AAK41543">
        <property type="protein sequence ID" value="AAK41543"/>
        <property type="gene ID" value="SSO1307"/>
    </dbReference>
    <dbReference type="GeneID" id="1454322"/>
    <dbReference type="KEGG" id="sso:SSO1307"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1308"/>
    <dbReference type="eggNOG" id="arCOG02266">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098915_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YL6"/>
    <dbReference type="PhylomeDB" id="Q97YL6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="28786" checksum="6F744BD2A2BA7C0B" modified="2001-10-01" version="1">MKCQIQYVELPNTFPKSDVDVYRVLEEDGKYYIRLELNGKPMYFTFSTSEIVLHSKELYELVSREEIDDCSPLGILDEIFYVILYQLGTKRTQLFLEFDNLFDLITSGKVKDSKRIILLRKMILTNYSDSTVIYYVSRRLSKFLTAESVEDVKFNYERAELLVTRSGDLYNIYLTEVQNELNEIIKKLTSISFIFMPITAVASIYAVDFTNVYSTLINWQSLIFLSPVLILTILLVIYLRKIRWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YP1</accession>
    <name>Q97YP1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Oligo/dipeptide transport, permease protein (DppB-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dppB-2</name>
        <name evidence="9" type="ordered locus">SSO1284</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90283">
        <property type="entry name" value="B90283"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YP1"/>
    <dbReference type="FunCoup" id="Q97YP1">
        <property type="interactions" value="38"/>
    </dbReference>
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    <dbReference type="PaxDb" id="273057-SSO1284"/>
    <dbReference type="EnsemblBacteria" id="AAK41521">
        <property type="protein sequence ID" value="AAK41521"/>
        <property type="gene ID" value="SSO1284"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1284"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1283"/>
    <dbReference type="eggNOG" id="arCOG00751">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_3_2"/>
    <dbReference type="InParanoid" id="Q97YP1"/>
    <dbReference type="PhylomeDB" id="Q97YP1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="entry name" value="MetI-like_sf"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN APPB"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
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    </dbReference>
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        <property type="entry name" value="MetI-like"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="113"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="333" mass="38042" checksum="269AFE181D665377" modified="2001-10-01" version="1">MIKSYMGFLNYAIKRAVDRIILLLGLVILLWFVVEALPELFGVNPAIWYAPVGQLGKSASYFQNIINDITTLYGFNQPLYVQFIRYLYALFSFNLGYDFQHHEYVINEVLQYLPYTIILTLPPFIFQTLLAIVVGAYAALKRNKAVDHIISNYMILQYNIPGFFILAVLWVVFAVYIKAIPISGIQVLHLNDIVSILKVYWLPWIIQVFIFGFPVRGILMRNTMVDVLDSDFVKYARLSGLKESTVRAIARRNSIIPVITRTGIDLAFILGGIYFIEYIFGIPGMGYLGIIAATELNIPLLTGSFFFLTLYALVVLYAMDLIYPLVDPRIKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YQ0</accession>
    <name>Q97YQ0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Oligo/dipeptide transport, permease protein (DppB-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dppB-1</name>
        <name evidence="9" type="ordered locus">SSO1274</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90281">
        <property type="entry name" value="H90281"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923280.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YQ0"/>
    <dbReference type="FunCoup" id="Q97YQ0">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1274"/>
    <dbReference type="PaxDb" id="273057-SSO1274"/>
    <dbReference type="EnsemblBacteria" id="AAK41511">
        <property type="protein sequence ID" value="AAK41511"/>
        <property type="gene ID" value="SSO1274"/>
    </dbReference>
    <dbReference type="GeneID" id="1454290"/>
    <dbReference type="KEGG" id="sso:SSO1274"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1271"/>
    <dbReference type="eggNOG" id="arCOG00751">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_0_2"/>
    <dbReference type="InParanoid" id="Q97YQ0"/>
    <dbReference type="PhylomeDB" id="Q97YQ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30465:SF45">
        <property type="entry name" value="BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEMS INNER MEMBRANE COMPONENT"/>
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    </dbReference>
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        <property type="entry name" value="INNER MEMBRANE ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="124"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="348" mass="38327" checksum="59E33C091288E1BE" modified="2001-10-01" version="1">MGFTTYMIKKVLIYFSVLLATLTILYIFTFPILQEVIAKSINFQVAQFSQTLFKNAHNLNSTQIQIAVEKYRESLIAAYGLNQPIIDKYFIQMYNLMRFDFGTAYFLQAPSGSREVSSIIAYYLPNTILLFTTATIVFIVAGTIIGLLSAKSKFWEKVIAIIAVIHSSIPTWWLGFVLIAALAYAVKVFPPGGMTSVPPPKNPFDYGISVLYHMALPLITIFIVNVGGFAYIVRSLVTSIMKEDFVITAKARGLPASRILYRHVLRSGSPSIATQAILALAGSLGGSLTTEVVFEWPGVGLLTYVAITLNDLPVILGITYVLTIVLLLGLFLGELVYGLLDPRIKVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YQ6</accession>
    <name>Q97YQ6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamine--fructose-6-phosphate transaminase (isomerizing)</fullName>
            <ecNumber evidence="2">2.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">glmS-2</name>
        <name evidence="10" type="ordered locus">SSO1268</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90281">
        <property type="entry name" value="B90281"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990024.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YQ6"/>
    <dbReference type="FunCoup" id="Q97YQ6">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1268"/>
    <dbReference type="MEROPS" id="C44.A08"/>
    <dbReference type="PaxDb" id="273057-SSO1268"/>
    <dbReference type="EnsemblBacteria" id="AAK41505">
        <property type="protein sequence ID" value="AAK41505"/>
        <property type="gene ID" value="SSO1268"/>
    </dbReference>
    <dbReference type="GeneID" id="1454284"/>
    <dbReference type="KEGG" id="sso:SSO1268"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1264"/>
    <dbReference type="eggNOG" id="arCOG00057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_5_2_2"/>
    <dbReference type="InParanoid" id="Q97YQ6"/>
    <dbReference type="PhylomeDB" id="Q97YQ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000144">
        <property type="entry name" value="Glutamine-fructose-6-phosphate transaminase (Isomerizing) (GlmS-2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="8">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="9">
        <location>
            <begin position="272"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="9">
        <location>
            <begin position="442"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="411"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK41505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="584" mass="63914" checksum="A502BE2868BE93D6" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YW5</accession>
    <name>Q97YW5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90272">
        <property type="entry name" value="D90272"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923234.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YW5"/>
    <dbReference type="PaxDb" id="273057-SSO1186"/>
    <dbReference type="EnsemblBacteria" id="AAK41435">
        <property type="protein sequence ID" value="AAK41435"/>
        <property type="gene ID" value="SSO1186"/>
    </dbReference>
    <dbReference type="GeneID" id="1454214"/>
    <dbReference type="KEGG" id="sso:SSO1186"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1183"/>
    <dbReference type="eggNOG" id="arCOG07239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1032971_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YW5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="269" mass="31270" checksum="AC24AAB838273E83" modified="2001-10-01" version="1">MKKPKILLSIIKSLLENNGHITSRQLPKSKKVPMNQLYKAILILNKLGLIQINGELNNNKFELHLIRNPEFLNLEQKVKVTFYEDRSKTKSFKFHSIVLKNGLLYIEDSSYWSADDKVKTKVKVYKPKREFGKVVVEKIELGTQKAYIKLALAPPTKVGEKIIHGFKVIQSNYFTFTQKETIERYGDPEMMDGLSVMQPTLFATLEIRFPESYRFIDVKAEKYDLVIIDGPQIPSPIPNHRLLIDRNKIVLKLFYPPFGYYFVSWKAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z24</accession>
    <name>Q97Z24_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetoin utilization protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90264">
        <property type="entry name" value="F90264"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z24"/>
    <dbReference type="FunCoup" id="Q97Z24">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1117"/>
    <dbReference type="PaxDb" id="273057-SSO1117"/>
    <dbReference type="EnsemblBacteria" id="AAK41373">
        <property type="protein sequence ID" value="AAK41373"/>
        <property type="gene ID" value="SSO1117"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1117"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1113"/>
    <dbReference type="eggNOG" id="arCOG00326">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_8_0_2"/>
    <dbReference type="InParanoid" id="Q97Z24"/>
    <dbReference type="PhylomeDB" id="Q97Z24"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="1">
        <location>
            <begin position="36"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="348" mass="39186" checksum="84249E44FD2D4FD6" modified="2001-10-01" version="1">MKVMRLTYQNSLGIVWDQRFTEISFSHPMIRDISKARVRDFIKLAKEKVSFVEIRPEYATKEDLMVVHTRDYIGLLEESSKIPYIGFLDQGDTVHYPGMFEDILLVLGSSFTAIKYSKFLDYVYIPLGGFHHAMPNRAVGFCPINDVAITALKLLEKGERVAIVDVDAHHGNGLQFILYDKPILKINIYAYDGNFFPGTGKIDEIGEGKGRGYNINIPLPLGSTDDIFEKSLEILDLLELYNPSYVLVVAGVDGHKNDQLKSLDLTTNSYNLLGLRIRRIKKTINSSIIAYGGGGYGPMSSLSMVSFLEGLLGRRTSYEPLTSSEDIGKIRRIEKIISRFKTLSNFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-11-27" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZC9</accession>
    <name>Q97ZC9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO0992</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99251">
        <property type="entry name" value="A99251"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923162.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZC9"/>
    <dbReference type="STRING" id="273057.SSO0992"/>
    <dbReference type="PaxDb" id="273057-SSO0992"/>
    <dbReference type="EnsemblBacteria" id="AAK41264">
        <property type="protein sequence ID" value="AAK41264"/>
        <property type="gene ID" value="SSO0992"/>
    </dbReference>
    <dbReference type="GeneID" id="1455231"/>
    <dbReference type="KEGG" id="sso:SSO0992"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.990"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_705185_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZC9"/>
    <dbReference type="PhylomeDB" id="Q97ZC9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17478">
        <property type="entry name" value="MFS_FsR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43129">
        <property type="entry name" value="FOSMIDOMYCIN RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43129:SF1">
        <property type="entry name" value="FOSMIDOMYCIN RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="5"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="396" mass="41999" checksum="AC9866E8AD4C6557" modified="2001-10-01" version="1">MKIRILTLTSLSHFINDGNSWVLPVTFIFLITYLGISKFLIGILSGAFFFGISALASPLVSKIADKFTNYSSIMGIGILLWGIGLISFGYSIQLHFLPLVFISVAIAGFASAFYHPIGAAVLSITYKGNAGIALGINGSMGSLGRAIYPTLTLSLFAILNKDMTLDMLIIGIISIIAALPSIFLKISITKEEDHKTPSSSNTTSTRGTLFVVILLTIIALLRSIFGQGISQFLPTLLVENYGYSYNVNLGEAITIALAAAIVGQPILGFLSDRVGRRLIYAISTFGAALTLLLFLKIPNIALLSLFGFFNFSAFPLMLSIVGDFVPRNSASFANSLVWGLGVTGGGVIGPIVVGAVSQVSNLVFASEIVTIMAFVAGALTALIPKPPKRTKVPLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZD4</accession>
    <name>Q97ZD4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0986</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90250">
        <property type="entry name" value="C90250"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989257.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZD4"/>
    <dbReference type="STRING" id="273057.SSO0986"/>
    <dbReference type="PaxDb" id="273057-SSO0986"/>
    <dbReference type="EnsemblBacteria" id="AAK41258">
        <property type="protein sequence ID" value="AAK41258"/>
        <property type="gene ID" value="SSO0986"/>
    </dbReference>
    <dbReference type="GeneID" id="1455225"/>
    <dbReference type="KEGG" id="sso:SSO0986"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.983"/>
    <dbReference type="eggNOG" id="arCOG03852">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1302565_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZD4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="27296" checksum="E4EF17DD0CD95928" modified="2001-10-01" version="1">MNIKNALERKDVKYLIRNIRSLLNLLKSKDGLEREVGWKAIDFLIETGNVNELEQYRNYLRSLLWHRLQGVRDDAWKHLHVYKILQTKGIERALTAQSDKIKWSAWSNVLKLIQLEIVPKEHIRSTRYAYWRLLRSIYPTIRKKAWRLFVKLVHEGIFDSSDKDRFSEFLKSKKANVRILAWRIAFMLVKENFISLDELKANIRYLEELTMQQSKVKKVAEKLIKELT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-09" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZF4</accession>
    <name>ALBA2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA/RNA-binding protein Alba 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">albA2</name>
        <name evidence="8 9" type="synonym">sso10b2</name>
        <name type="ordered locus">SSO6877</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="Nat. Commun." volume="3" first="1328" last="1328">
            <title>Alba shapes the archaeal genome using a delicate balance of bridging and stiffening the DNA.</title>
            <authorList>
                <person name="Laurens N."/>
                <person name="Driessen R.P."/>
                <person name="Heller I."/>
                <person name="Vorselen D."/>
                <person name="Noom M.C."/>
                <person name="Hol F.J."/>
                <person name="White M.F."/>
                <person name="Dame R.T."/>
                <person name="Wuite G.J."/>
            </authorList>
            <dbReference type="PubMed" id="23271660"/>
            <dbReference type="DOI" id="10.1038/ncomms2330"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH ALBA1</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="journal article" date="2022" name="Biomolecules" volume="12" first="0" last="0">
            <title>Interplay between Alba and Cren7 Regulates Chromatin Compaction in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Cajili M.K.M."/>
                <person name="Prieto E.I."/>
            </authorList>
            <dbReference type="PubMed" id="35454068"/>
            <dbReference type="DOI" id="10.3390/biom12040481"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH ALBA1</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="journal article" date="2003" name="J. Bacteriol." volume="185" first="4066" last="4073">
            <title>Crystal structure of the hyperthermophilic archaeal DNA-binding protein Sso10b2 at a resolution of 1.85 Angstroms.</title>
            <authorList>
                <person name="Chou C.C."/>
                <person name="Lin T.W."/>
                <person name="Chen C.Y."/>
                <person name="Wang A.H."/>
            </authorList>
            <dbReference type="PubMed" id="12837780"/>
            <dbReference type="DOI" id="10.1128/jb.185.14.4066-4073.2003"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH ZINC ION</scope>
        <scope>FUNCTION</scope>
    </reference>
    <reference evidence="12" key="5">
        <citation type="journal article" date="2005" name="Biochemistry" volume="44" first="14217" last="14230">
            <title>Solution structure, stability, and nucleic acid binding of the hyperthermophile protein Sso10b2.</title>
            <authorList>
                <person name="Biyani K."/>
                <person name="Kahsai M.A."/>
                <person name="Clark A.T."/>
                <person name="Armstrong T.L."/>
                <person name="Edmondson S.P."/>
                <person name="Shriver J.W."/>
            </authorList>
            <dbReference type="PubMed" id="16245938"/>
            <dbReference type="DOI" id="10.1021/bi051266r"/>
        </citation>
        <scope>STRUCTURE BY NMR</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference evidence="13" key="6">
        <citation type="journal article" date="2005" name="Structure" volume="13" first="963" last="971">
            <title>Obligate heterodimerization of the archaeal Alba2 protein with Alba1 provides a mechanism for control of DNA packaging.</title>
            <authorList>
                <person name="Jelinska C."/>
                <person name="Conroy M.J."/>
                <person name="Craven C.J."/>
                <person name="Hounslow A.M."/>
                <person name="Bullough P.A."/>
                <person name="Waltho J.P."/>
                <person name="Taylor G.L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="16004869"/>
            <dbReference type="DOI" id="10.1016/j.str.2005.04.016"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS)</scope>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH ALBA1</scope>
    </reference>
    <comment type="function">
        <text evidence="3 4 5 6 7">Binds single-stranded DNA, RNA and double-stranded DNA (PubMed:12837780, PubMed:16245938). Involved in DNA compaction (PubMed:16004869, PubMed:23271660, PubMed:35454068).</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5 6 7">Forms homodimers and homotetramers (PubMed:16245938). Homodimer at pH below 6.0 (PubMed:16245938). Forms homotetramers and higher order homooligomers at near the growth temperature of 80 degrees Celsius and pH 7.0 (PubMed:16245938). Interacts with Alba 1; heterodimers lack cooperative DNA-binding behavior and result in more compact chromatin structures compared to Alba 1 homodimers (PubMed:16004869, PubMed:23271660, PubMed:35454068).</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-9021196">
            <id>Q97ZF4</id>
        </interactant>
        <interactant intactId="EBI-9021190">
            <id>P60849</id>
            <label>albA1</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>6</experiments>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 2">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="2">Acetylated. Acetylation at Lys-12 decreases DNA-binding affinity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the histone-like Alba family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90247">
        <property type="entry name" value="G90247"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992410.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1UDV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.85 A"/>
        <property type="chains" value="A/B=1-89"/>
    </dbReference>
    <dbReference type="PDB" id="2A2Y">
        <property type="method" value="NMR"/>
        <property type="chains" value="A/B=1-89"/>
    </dbReference>
    <dbReference type="PDB" id="2BKY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="X/Y=1-89"/>
    </dbReference>
    <dbReference type="PDBsum" id="1UDV"/>
    <dbReference type="PDBsum" id="2A2Y"/>
    <dbReference type="PDBsum" id="2BKY"/>
    <dbReference type="AlphaFoldDB" id="Q97ZF4"/>
    <dbReference type="BMRB" id="Q97ZF4"/>
    <dbReference type="SMR" id="Q97ZF4"/>
    <dbReference type="DIP" id="DIP-48445N"/>
    <dbReference type="FunCoup" id="Q97ZF4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="IntAct" id="Q97ZF4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO6877"/>
    <dbReference type="PaxDb" id="273057-SSO6877"/>
    <dbReference type="EnsemblBacteria" id="AAK41238">
        <property type="protein sequence ID" value="AAK41238"/>
        <property type="gene ID" value="SSO6877"/>
    </dbReference>
    <dbReference type="GeneID" id="1455205"/>
    <dbReference type="GeneID" id="44129895"/>
    <dbReference type="KEGG" id="sso:SSO6877"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.962"/>
    <dbReference type="eggNOG" id="arCOG01753">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110989_2_0_2"/>
    <dbReference type="InParanoid" id="Q97ZF4"/>
    <dbReference type="PhylomeDB" id="Q97ZF4"/>
    <dbReference type="EvolutionaryTrace" id="Q97ZF4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01122">
        <property type="entry name" value="AlbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013795">
        <property type="entry name" value="DNA/RNA-bd_Alba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028732">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0007">Acetylation</keyword>
    <keyword id="KW-0158">Chromosome</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0226">DNA condensation</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000151713" description="DNA/RNA-binding protein Alba 2">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 11">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 11">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 11">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="2">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="strand" evidence="15">
        <location>
            <begin position="6"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="strand" evidence="14">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="helix" evidence="15">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="strand" evidence="15">
        <location>
            <begin position="33"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="helix" evidence="15">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="helix" evidence="15">
        <location>
            <begin position="58"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="strand" evidence="15">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="strand" evidence="15">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P60849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12837780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="16004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="16245938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="23271660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="35454068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="8">
        <source>
            <dbReference type="PubMed" id="12837780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="9">
        <source>
            <dbReference type="PubMed" id="16245938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="10"/>
    <evidence type="ECO:0007744" key="11">
        <source>
            <dbReference type="PDB" id="1UDV"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="12">
        <source>
            <dbReference type="PDB" id="2A2Y"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="13">
        <source>
            <dbReference type="PDB" id="2BKY"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="14">
        <source>
            <dbReference type="PDB" id="1UDV"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="15">
        <source>
            <dbReference type="PDB" id="2BKY"/>
        </source>
    </evidence>
    <sequence length="89" mass="10239" checksum="A945B8C63E8AA02B" modified="2001-10-01" version="1">MTEKLNEIVVRKTKNVEDHVLDVIVLFNQGIDEVILKGTGREISKAVDVYNSLKDRLGDGVQLVNVQTGSEVRDRRRISYILLRLKRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZV2</accession>
    <name>Q97ZV2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA repair protein rad25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO0473</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90192">
        <property type="entry name" value="E90192"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922990.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZV2"/>
    <dbReference type="FunCoup" id="Q97ZV2">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0473"/>
    <dbReference type="PaxDb" id="273057-SSO0473"/>
    <dbReference type="EnsemblBacteria" id="AAK40796">
        <property type="protein sequence ID" value="AAK40796"/>
        <property type="gene ID" value="SSO0473"/>
    </dbReference>
    <dbReference type="GeneID" id="7807122"/>
    <dbReference type="KEGG" id="sso:SSO0473"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.469"/>
    <dbReference type="eggNOG" id="arCOG00874">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008213_6_0_2"/>
    <dbReference type="InParanoid" id="Q97ZV2"/>
    <dbReference type="PhylomeDB" id="Q97ZV2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17926">
        <property type="entry name" value="DEXHc_RE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050615">
        <property type="entry name" value="ATP-dep_DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040699">
        <property type="entry name" value="XPB_DRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274">
        <property type="entry name" value="RAD25/XP-B DNA REPAIR HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18458">
        <property type="entry name" value="XPB_DRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="77"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="314"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="444" mass="50817" checksum="16B3E1752BB59D45" modified="2001-10-01" version="1">MVRLRYFKCLLLSDFSAPGLSWNDEFKCYIGQANKYREVLYYYKRSHVEVEDSVLELLPFPLIVDRIKLREYQQEALNAWLKSKLGIIVIPTGGGKTVIGLKAIALIRLATLIIVPTIDLLQQWYENIRELLGVEAGRVGGGYDELKGITVITYDSAYTQLEKIGNKFGLVIFDEVHHLPSEGYSIIAQGLAAPYRLGLTATPERSDGRHKLYPSLVGPLVYRITVSNLVGKYLSSFEIQRIYVNLTEDEEKLYRHYRGILKKFLSQRNLKLKSLNDFNRLLRLAVKDKEAREALLAWHEALKIAVNSKAKLEKLKDLLKELNGEKIIIFTRNTSMVYEISRLFLIPAVTYKTNKQERIEILEKFRSGKYNVIVTSSVLDEGIDVPDASIGIVLGGYGTSRQFIQRLGRILRKKENKKARLIEIITKGTSDYNLSKRRRQNANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZX3</accession>
    <name>Q97ZX3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GtrA/DPMS transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO0449</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GtrA family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90189">
        <property type="entry name" value="H90189"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988712.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZX3"/>
    <dbReference type="STRING" id="273057.SSO0449"/>
    <dbReference type="PaxDb" id="273057-SSO0449"/>
    <dbReference type="EnsemblBacteria" id="AAK40775">
        <property type="protein sequence ID" value="AAK40775"/>
        <property type="gene ID" value="SSO0449"/>
    </dbReference>
    <dbReference type="GeneID" id="1455585"/>
    <dbReference type="KEGG" id="sso:SSO0449"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.443"/>
    <dbReference type="eggNOG" id="arCOG02228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1880932_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZX3"/>
    <dbReference type="PhylomeDB" id="Q97ZX3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051401">
        <property type="entry name" value="GtrA_CellWall_Glycosyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007267">
        <property type="entry name" value="GtrA_DPMS_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38459">
        <property type="entry name" value="PROPHAGE BACTOPRENOL-LINKED GLUCOSE TRANSLOCASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38459:SF1">
        <property type="entry name" value="PROPHAGE BACTOPRENOL-LINKED GLUCOSE TRANSLOCASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04138">
        <property type="entry name" value="GtrA_DPMS_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="GtrA/DPMS transmembrane" evidence="7">
        <location>
            <begin position="9"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK40775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="145" mass="16112" checksum="919274ECCD7FDB8F" modified="2001-10-01" version="1">MSLLIRLAKFALVGGLGTIVNEVTYVLASKTIPIGVSLAIAIEISLIFNFVLNDIWTFKDKRNNSYLNRLLKFHGSSYLGNIVQYIVALVLLVYLLHISSISDAVFILFFSKLAASTFTLVLTNFIGIVSGFVVRFITSLKYVWA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-15" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q980E8</accession>
    <name>GCH1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="2">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">GTP cyclohydrolase I</fullName>
            <shortName evidence="2">GTP-CH-I</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">folE</name>
        <name type="ordered locus">SSO0364</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17473"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="EC" id="3.5.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Toroid-shaped homodecamer, composed of two pentamers of five dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90179">
        <property type="entry name" value="G90179"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990665.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980E8"/>
    <dbReference type="SMR" id="Q980E8"/>
    <dbReference type="FunCoup" id="Q980E8">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0364"/>
    <dbReference type="PaxDb" id="273057-SSO0364"/>
    <dbReference type="EnsemblBacteria" id="AAK40694">
        <property type="protein sequence ID" value="AAK40694"/>
        <property type="gene ID" value="SSO0364"/>
    </dbReference>
    <dbReference type="GeneID" id="1455504"/>
    <dbReference type="GeneID" id="44129338"/>
    <dbReference type="KEGG" id="sso:SSO0364"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.358"/>
    <dbReference type="eggNOG" id="arCOG04542">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049768_3_2_2"/>
    <dbReference type="InParanoid" id="Q980E8"/>
    <dbReference type="PhylomeDB" id="Q980E8"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.286.10:FF:000007">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1130.10:FF:000001">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006825">
        <property type="entry name" value="PRK09347.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006826">
        <property type="entry name" value="PRK09347.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109:SF7">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="GTP CYCLOHYDROLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00860">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000119475" description="GTP cyclohydrolase 1">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00223"/>
        </source>
    </evidence>
    <sequence length="208" mass="23578" checksum="EA37486D88C543B1" modified="2001-10-01" version="1">MQKTELDDQKLVEEIARRIREILELLGENPEREGLKETPERVAKALLEMTSGLRTPPPQIKVFNLGDDGEVYEKNQIVLIKDVNFSSLCEHHMLPIIGKIHVAYIVSNSGKVAGFSKIIRIVNYYSSRLQIQERLVEQIADAIMNSEIKPKGVMVIGNAIHMCSYVRGVKDKEAKLVSVAYRGLFKTNRALQNHVFRLLDNANKVNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q980G4</accession>
    <name>Q980G4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90176">
        <property type="entry name" value="E90176"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980G4"/>
    <dbReference type="STRING" id="273057.SSO0335"/>
    <dbReference type="PaxDb" id="273057-SSO0335"/>
    <dbReference type="EnsemblBacteria" id="AAK40668">
        <property type="protein sequence ID" value="AAK40668"/>
        <property type="gene ID" value="SSO0335"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0335"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.329"/>
    <dbReference type="eggNOG" id="arCOG03704">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_822878_0_0_2"/>
    <dbReference type="InParanoid" id="Q980G4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053108">
        <property type="entry name" value="Chlamydial_TARP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36975">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36975:SF5">
        <property type="entry name" value="TRANSLOCATED ACTIN-RECRUITING PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="337" mass="37792" checksum="06F6D605320D448D" modified="2001-10-01" version="1">MGKNFLNKFQLSSRSKMADMKTIAFSIVAVVLIVIAAIGFYEYSVANSRYTSAYNEYQSVDSQYMALQGNYTKLNQTYSQLASQYKLLEGMYNAVLTEAKNNYTLYRQAETNYTYYKTLYEQMLQQISKLNVSGYKPGAALSAVLQFYDGIAIESPSDVVPLLASNFTAYIMGQPFAGTYNLTTFNTTWLANFFGTYETVYFYTTALPTVTQINGNTFNVTDVVQYFVAPTADPVYLQVFNASNTIIVQYIHGVPEITELIWKGNEVPPSAVITGYPSQHLIQANQVLDEFLSQMNALGAEFPGSTIAQNFAPNAQLVLIGQLLRYLKRELILEFLV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-03" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q980G6</accession>
    <name>THII_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable tRNA sulfurtransferase</fullName>
            <ecNumber evidence="1">2.8.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Sulfur carrier protein ThiS sulfurtransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thiamine biosynthesis protein ThiI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA 4-thiouridine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiI</name>
        <name type="ordered locus">SSO0333</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + ATP + H(+) = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + 2 oxidized [2Fe-2S]-[ferredoxin] + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:24176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:13337"/>
            <dbReference type="Rhea" id="RHEA-COMP:13338"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136798"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[ThiS sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 = [ThiS sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43340"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:12910"/>
            <dbReference type="Rhea" id="RHEA-COMP:19908"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:232372"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiI family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.4" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90176">
        <property type="entry name" value="C90176"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990625.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980G6"/>
    <dbReference type="SMR" id="Q980G6"/>
    <dbReference type="FunCoup" id="Q980G6">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0333"/>
    <dbReference type="PaxDb" id="273057-SSO0333"/>
    <dbReference type="EnsemblBacteria" id="AAK40666">
        <property type="protein sequence ID" value="AAK40666"/>
        <property type="gene ID" value="SSO0333"/>
    </dbReference>
    <dbReference type="GeneID" id="1455476"/>
    <dbReference type="GeneID" id="44129306"/>
    <dbReference type="KEGG" id="sso:SSO0333"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.326"/>
    <dbReference type="eggNOG" id="arCOG00038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037952_4_0_2"/>
    <dbReference type="InParanoid" id="Q980G6"/>
    <dbReference type="PhylomeDB" id="Q980G6"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990228">
        <property type="term" value="C:sulfurtransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140741">
        <property type="term" value="F:tRNA-uracil-4 sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002937">
        <property type="term" value="P:tRNA 4-thiouridine biosynthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01712">
        <property type="entry name" value="PPase_ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11716">
        <property type="entry name" value="THUMP_ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000053">
        <property type="entry name" value="Probable tRNA sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00021">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054173">
        <property type="entry name" value="ThiI_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049961">
        <property type="entry name" value="ThiI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049962">
        <property type="entry name" value="THUMP_ThiI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003720">
        <property type="entry name" value="tRNA_STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050102">
        <property type="entry name" value="tRNA_sulfurtransferase_ThiI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00342">
        <property type="entry name" value="tRNA uracil 4-sulfurtransferase ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209:SF1">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22025">
        <property type="entry name" value="ThiI_fer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000154900" description="Probable tRNA sulfurtransferase">
        <location>
            <begin position="1"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="THUMP" evidence="1">
        <location>
            <begin position="54"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="174"/>
            <end position="175"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="199"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00021"/>
        </source>
    </evidence>
    <sequence length="371" mass="41848" checksum="6D9F628AEEB63F44" modified="2001-10-01" version="1">MLVIIRPSGEIALKSPRSRRNFEYTLINNIRNTIGGGKIWRSQGVILLEVNDNNANIEALSEVFGISSFSPVIAIKSNNLEDIVNKAKEIFAEIVKGKIFAVRAKRIGSHSFTSLDVERRAGEALYPYSKGVDLENPEVEIFIEIRNEMTYFYHKVIKGPKGLPVGVAGKTVVLFSGGIDSPVATWMMMKRGSVPIILNFNLGGDLHKELVLKELNMLKRWSGGHKLKIFIVKGTDVFIKLSQVERRSRVVMLKRVMYKTAEKLCEKTNAKSITTGESLSQVSSQTMANLYVTEYGIKYPIFRPLIGFDKEEIVDIARKIGTYKHSIKLPEYCAISTKARTSEDLNEILKNEERLNVDYEKILENSEVIEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2021-09-29" modified="2025-06-18" version="138" xmlns="https://uniprot.org/uniprot">
    <accession>Q980K2</accession>
    <name>TFS1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription factor S1</fullName>
            <shortName evidence="6">TFS1</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Transcript cleavage factor TFS1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tfs1</name>
        <name evidence="5" type="synonym">rpoM-1</name>
        <name type="ordered locus">SSO0291</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2017" name="Nat. Commun." volume="8" first="1914" last="1914">
            <title>The transcript cleavage factor paralogue TFS4 is a potent RNA polymerase inhibitor.</title>
            <authorList>
                <person name="Fouqueau T."/>
                <person name="Blombach F."/>
                <person name="Hartman R."/>
                <person name="Cheung A.C.M."/>
                <person name="Young M.J."/>
                <person name="Werner F."/>
            </authorList>
            <dbReference type="PubMed" id="29203770"/>
            <dbReference type="DOI" id="10.1038/s41467-017-02081-3"/>
        </citation>
        <scope>FUNCTION IN TRANSCRIPT CLEAVAGE</scope>
        <scope>PROBABLE COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>DOMAIN</scope>
        <scope>ACTIVE SITE</scope>
        <scope>MUTAGENESIS OF 84-TYR--THR-98 AND 94-ASP-GLU-95</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 4">Induces RNA cleavage activity in the RNA polymerase. Induces rapid cleavage of a stalled transcription elongation complex with a 2-nucleotide reduction at the 3' end of the nascent RNA. Truncated RNA is able to resume elongation. During transcription elongation it enhances processivity (PubMed:29203770). Involved in transcriptional proofreading and fidelity. Misincorporation of nucleotides during elongation of transcription leads to arrested elongation complexes which are rescued by TFS-promoted removal of a dinucleotide from the 3'-end. TFS1 is able to induce a cleavage resynthesis cycle in stalled elongation complexes (resulting from the next missing nucleotide or a reduced incorporation rate of a wrong nucleotide) preventing misincorporation and enabling proofreading in a post-incorporation manner. Pausing of elongation complexes is the main determinant of TFS-induced RNA cleavage.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with RNA polymerase; probably competes with TFS4 for the same binding site.</text>
    </comment>
    <comment type="domain">
        <text evidence="4 8">Has 2 zinc-ribbon domains (N-ZR and C-ZR); functionally important residues Asp-94 and Glu-95 are probably located at the tip of a domain that extends from C-ZR into the RNAP active site. The nature of these residues determines if the protein is involved in stalled transcript cleavage and thus activation (Asp and Glu residues in this protein, TFS1) or in RNAP inhibition (Lys residues in TFS4).</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90171">
        <property type="entry name" value="F90171"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980K2"/>
    <dbReference type="SMR" id="Q980K2"/>
    <dbReference type="FunCoup" id="Q980K2">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0291"/>
    <dbReference type="PaxDb" id="273057-SSO0291"/>
    <dbReference type="EnsemblBacteria" id="AAK40629">
        <property type="protein sequence ID" value="AAK40629"/>
        <property type="gene ID" value="SSO0291"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0291"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.284"/>
    <dbReference type="eggNOG" id="arCOG00579">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093932_3_2_2"/>
    <dbReference type="InParanoid" id="Q980K2"/>
    <dbReference type="PhylomeDB" id="Q980K2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd10511">
        <property type="entry name" value="Zn-ribbon_TFS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
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    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
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    <dbReference type="InterPro" id="IPR006288">
        <property type="entry name" value="TFS"/>
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        <property type="entry name" value="Zn_ribbon_RPB9"/>
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    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01384">
        <property type="entry name" value="TFS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0010">Activator</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000453929" description="Transcription factor S1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TFIIS-type" evidence="2">
        <location>
            <begin position="71"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-ZR" evidence="8">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-ZR" evidence="8">
        <location>
            <begin position="63"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 8">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 8">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 8">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 8">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 8">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 8">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 8">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 8">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Inhibits RNAP activity, destabilizes pre-initiation complex and inhibits transcription initiation (TFS1-tip4, with active tip of TFS4)." evidence="4">
        <original>YFWILQTRRADEPPT</original>
        <variation>ILLNKKK</variation>
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of RNA cleavage activity, reduces transcription processivity." evidence="4">
        <original>DE</original>
        <variation>AA</variation>
        <location>
            <begin position="94"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9P9I8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10145"/>
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    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="29203770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="11427726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="6">
        <source>
            <dbReference type="PubMed" id="29203770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <evidence type="ECO:0000305" key="8">
        <source>
            <dbReference type="PubMed" id="29203770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK40629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="114" mass="13206" checksum="468A4680CA4AEED2" modified="2001-10-01" version="1">MIVVKFCPKCNSMMVPKKSNGKNVYRCTKCGYEKEVPETTIVVTSKVKHSIKEKTLVLEEEEMPSGAQKIKGVLCPSCKNDEAYFWILQTRRADEPPTRFYKCTKCGKVWREYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-19" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q980K7</accession>
    <name>RS17E_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein eS17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">30S ribosomal protein S17e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 5" type="primary">rps17e</name>
        <name type="ordered locus">SSO5561</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Located near the mRNA exit channel, it binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90171">
        <property type="entry name" value="A90171"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990569.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="S=1-79"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="S=1-79"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="S=1-79"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="S=1-79"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="S=1-79"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="S=1-79"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="S=1-79"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="S=1-79"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q980K7"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q980K7"/>
    <dbReference type="FunCoup" id="Q980K7">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO5561"/>
    <dbReference type="PaxDb" id="273057-SSO5561"/>
    <dbReference type="EnsemblBacteria" id="AAK40624">
        <property type="protein sequence ID" value="AAK40624"/>
        <property type="gene ID" value="SSO5561"/>
    </dbReference>
    <dbReference type="GeneID" id="1455434"/>
    <dbReference type="KEGG" id="sso:SSO5561"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.280"/>
    <dbReference type="eggNOG" id="arCOG01885">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176720_0_0_2"/>
    <dbReference type="InParanoid" id="Q980K7"/>
    <dbReference type="PhylomeDB" id="Q980K7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.20">
        <property type="entry name" value="Ribosomal protein S17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00511">
        <property type="entry name" value="Ribosomal_eS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018273">
        <property type="entry name" value="Ribosomal_eS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036401">
        <property type="entry name" value="Ribosomal_eS17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002242">
        <property type="entry name" value="PRK01151.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116820">
        <property type="entry name" value="Rps17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00712">
        <property type="entry name" value="RIBOSOMAL_S17E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000141566" description="Small ribosomal subunit protein eS17">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="79" mass="9628" checksum="F0BD0D1A9720D077" modified="2001-10-01" version="1">MGNIYTKDIKRIVKEIYDRYKDEIKDDYNTNKQIVIRYVDVKSKKVRNRIAGYLTRYYKIMKEKETSPAEEKEEISEEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q980N5</accession>
    <name>Y256_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0215 protein SSO0256</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0256</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0215 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90167">
        <property type="entry name" value="D90167"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990520.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980N5"/>
    <dbReference type="SMR" id="Q980N5"/>
    <dbReference type="PaxDb" id="273057-SSO0256"/>
    <dbReference type="EnsemblBacteria" id="AAK40595">
        <property type="protein sequence ID" value="AAK40595"/>
        <property type="gene ID" value="SSO0256"/>
    </dbReference>
    <dbReference type="GeneID" id="1455405"/>
    <dbReference type="KEGG" id="sso:SSO0256"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.250"/>
    <dbReference type="eggNOG" id="arCOG00928">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095956_1_1_2"/>
    <dbReference type="InParanoid" id="Q980N5"/>
    <dbReference type="PhylomeDB" id="Q980N5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2170.10">
        <property type="entry name" value="archaeoglobus fulgidus dsm 4304 superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00582">
        <property type="entry name" value="UPF0215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002802">
        <property type="entry name" value="Endo_dU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39518">
        <property type="entry name" value="UPF0215 PROTEIN MJ1150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39518:SF2">
        <property type="entry name" value="UPF0215 PROTEIN MJ1150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01949">
        <property type="entry name" value="Endo_dU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006380">
        <property type="entry name" value="UCP006380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000149244" description="UPF0215 protein SSO0256">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00582"/>
        </source>
    </evidence>
    <sequence length="179" mass="20539" checksum="8781272EF6931826" modified="2001-10-01" version="1">MLISGVDDGYFPLSYKGGKGKTALVVVTFYDYEIIDLDWGFITVDGNDATNVFKQLRKGDIVILDGVIFAGFNYIVPYSDRMIFFYSKMPKIDLIKKTLMKHFQSDTERVREILYVLNNLKQIPTKRGNVFLYSTVELSLAKSIIEKYQIYSKIPEVLKSAHIIASSLGRFLAHYKKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q980P9</accession>
    <name>Q980P9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thiamine monophosphate kinase, putative (ThiL)</fullName>
            <ecNumber evidence="2">2.7.4.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">thiL</name>
        <name evidence="2" type="ordered locus">SSO0237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.4.16" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90165">
        <property type="entry name" value="D90165"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980P9"/>
    <dbReference type="SMR" id="Q980P9"/>
    <dbReference type="FunCoup" id="Q980P9">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0237"/>
    <dbReference type="PaxDb" id="273057-SSO0237"/>
    <dbReference type="DNASU" id="1455389"/>
    <dbReference type="EnsemblBacteria" id="AAK40579">
        <property type="protein sequence ID" value="AAK40579"/>
        <property type="gene ID" value="SSO0237"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0237"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.234"/>
    <dbReference type="eggNOG" id="arCOG00638">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046964_2_1_2"/>
    <dbReference type="InParanoid" id="Q980P9"/>
    <dbReference type="PhylomeDB" id="Q980P9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02194">
        <property type="entry name" value="ThiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="THIAMINE-MONOPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270:SF0">
        <property type="entry name" value="THIAMINE-MONOPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005303">
        <property type="entry name" value="Thiam_monoph_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="1">
        <location>
            <begin position="44"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="323" mass="36986" checksum="D494D9819A58927E" modified="2001-10-01" version="1">MISNLYFVKLKNIGEHEFIKRYIKNYMDIKLLDDVFINEKRGYKVDGFKLSYAFPFMSLYDIGWKAISASTSDIIAKGVKPEFYLISLGLPPDFDVNKAEELIGGISDAIHYYGGRYVGGDLNDSDSNGWVDVFVEGEVLCDISDKVIDDGDLLVIGDYIGYTTNVFISYINNFNIQILPESLLKVKHPIVKKSLLFFMKKYCKFIKYSTDISDGLMVSLYKIVDNFNLGINITEIPIDKNVFNSLKLRLNLTELDILKYAGEEFLPLLIIDKNSPVKEMLAELQYLAFNPLIIGQVIHENRLITYKNTVIKNTGWDNFIGWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>Q980T1</accession>
    <name>Q980T1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">SSO0197</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90160">
        <property type="entry name" value="G90160"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990436.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980T1"/>
    <dbReference type="SMR" id="Q980T1"/>
    <dbReference type="FunCoup" id="Q980T1">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0197"/>
    <dbReference type="PaxDb" id="273057-SSO0197"/>
    <dbReference type="EnsemblBacteria" id="AAK40542">
        <property type="protein sequence ID" value="AAK40542"/>
        <property type="gene ID" value="SSO0197"/>
    </dbReference>
    <dbReference type="GeneID" id="1455352"/>
    <dbReference type="KEGG" id="sso:SSO0197"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.196"/>
    <dbReference type="eggNOG" id="arCOG01181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018693_1_0_2"/>
    <dbReference type="InParanoid" id="Q980T1"/>
    <dbReference type="PhylomeDB" id="Q980T1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05144">
        <property type="entry name" value="RIO2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030484">
        <property type="entry name" value="Rio2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852">
        <property type="entry name" value="SER/THR-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="94"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAK40542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="287" mass="33154" checksum="0BF7B4E6FB2091BA" modified="2001-10-01" version="1">MRLSLAEKASLVRPFDYILLKTIYGLRDKSEFVSFNALKKRLDIKDDEELKISLKKLSELKLISKKPTDLSFKLTFSGLDILGIKLLYVNKILNRLAEIIGIGKESLVYYGYDFNDNKIIVKFHRVGTDSYKKVKFRKSQEKKSWLSITVENAKREFEALTCLSNEGGYVPKPLGVEYNAVAMEYIDGIELYKIPVTNMDLNLDEILEKILQTMRIAYTICHITHGDLSPYNVLIDKNGNPYLIDWPQATKSEERLEKDLSNLIIFFRKKGIDVDTRKIFDYVRGVS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-29" modified="2026-01-28" version="138" xmlns="https://uniprot.org/uniprot">
    <accession>Q980V3</accession>
    <name>SYDND_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate--tRNA(Asp/Asn) ligase</fullName>
            <ecNumber evidence="1">6.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">ND-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aspS</name>
        <name type="ordered locus">SSO0173</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Asx) + L-aspartate + ATP = L-aspartyl-tRNA(Asx) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18349"/>
            <dbReference type="Rhea" id="RHEA-COMP:9710"/>
            <dbReference type="Rhea" id="RHEA-COMP:9711"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 3 Mg(2+) cations per subunit. The strongest magnesium site (Mg1) is bound to the beta- and gamma-phosphates of ATP and four water molecules complete its coordination sphere.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90157">
        <property type="entry name" value="H90157"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990398.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980V3"/>
    <dbReference type="SMR" id="Q980V3"/>
    <dbReference type="FunCoup" id="Q980V3">
        <property type="interactions" value="349"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0173"/>
    <dbReference type="PaxDb" id="273057-SSO0173"/>
    <dbReference type="EnsemblBacteria" id="AAK40519">
        <property type="protein sequence ID" value="AAK40519"/>
        <property type="gene ID" value="SSO0173"/>
    </dbReference>
    <dbReference type="GeneID" id="1453346"/>
    <dbReference type="GeneID" id="44129139"/>
    <dbReference type="KEGG" id="sso:SSO0173"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.169"/>
    <dbReference type="eggNOG" id="arCOG00406">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004553_2_1_2"/>
    <dbReference type="InParanoid" id="Q980V3"/>
    <dbReference type="PhylomeDB" id="Q980V3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050560">
        <property type="term" value="F:aspartate-tRNA(Asn) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000038">
        <property type="entry name" value="Aspartate--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000111006" description="Aspartate--tRNA(Asp/Asn) ligase">
        <location>
            <begin position="1"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="1">
        <location>
            <begin position="189"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="210"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="400"/>
            <end position="403"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="1">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02075"/>
        </source>
    </evidence>
    <sequence length="429" mass="48769" checksum="13EC8C220DCBEAFA" modified="2001-10-01" version="1">MFRTHLVSELNPKLDGSEVKVAGWVHNVRNLGGKIFILLRDKSGIGQIVVEKGNNAYDKVINIGLESTIVVNGVVKADARAPNGVEVHAKDIEILSYARSPLPLDVTGKVKADIDTRLRERLLDLRRLEMQAVLKIQSVAVKSFRETLYKHGFVEVFTPKIIASATEGGAQLFPVLYFGKEAFLAQSPQLYKELLAGAIERVFEIAPAWRAEESDTPYHLSEFISMDVEMAFADYNDIMALIEQIIYNMINDVKRECENELKILNYTPPNVRIPIKKVSYSDAIELLKSKGVNIKFGDDIGTPELRVLYNELKEDLYFVTDWPWLSRPFYTKQKKDNPQLSESFDLIFRWLEIVSGSSRNHVKEVLENSLKVRGLNPESFEFFLKWFDYGMPPHAGFGMGLARVMLMLTGLQSVKEVVPFPRDKKRLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Z9</accession>
    <name>Q980Z9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enoyl CoA hydratase (PaaF-1)</fullName>
            <ecNumber evidence="2">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">paaF-1</name>
        <name evidence="2" type="ordered locus">SSO0050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90144">
        <property type="entry name" value="H90144"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988849.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Z9"/>
    <dbReference type="STRING" id="273057.SSO0050"/>
    <dbReference type="PaxDb" id="273057-SSO0050"/>
    <dbReference type="EnsemblBacteria" id="AAK40415">
        <property type="protein sequence ID" value="AAK40415"/>
        <property type="gene ID" value="SSO0050"/>
    </dbReference>
    <dbReference type="GeneID" id="1455306"/>
    <dbReference type="KEGG" id="sso:SSO0050"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.53"/>
    <dbReference type="eggNOG" id="arCOG00242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_3_2"/>
    <dbReference type="InParanoid" id="Q980Z9"/>
    <dbReference type="PhylomeDB" id="Q980Z9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051683">
        <property type="entry name" value="Enoyl-CoA_Hydratase/Isomerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42964">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42964:SF1">
        <property type="entry name" value="POLYKETIDE BIOSYNTHESIS ENOYL-COA HYDRATASE PKSH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="249" mass="28466" checksum="AC4CB0EC504C3062" modified="2001-10-01" version="1">MPIEVYEYDKYILLKFYQADNRYNLFNVEFMTQMIDVLSAINENKGKRFLVIRGEDNFGAGADIRELIKASNDSEFAVTFFTYMREVFHKMLDLNKIVISQVKKIAYGASMELLLFSDYVISEKKAMFATPGVKIGVFPPVLSSVGHFILGYNNVKRIAMKGDVIDTNEAKSIGLVHIIDDDLDKATLDLIEELSTLAPSATLYIKRNMLRTFRNYIDKAFDDLIVQIQSEEAREGLLSFLNKTNPPWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q981A3</accession>
    <name>Q981A3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Terminal oxidase, small hydrophobic subunit (DoxE)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">doxE</name>
        <name evidence="2" type="ordered locus">SSO5098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90144">
        <property type="entry name" value="D90144"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981A3"/>
    <dbReference type="STRING" id="273057.SSO5098"/>
    <dbReference type="PaxDb" id="273057-SSO5098"/>
    <dbReference type="EnsemblBacteria" id="AAK40411">
        <property type="protein sequence ID" value="AAK40411"/>
        <property type="gene ID" value="SSO5098"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO5098"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.47"/>
    <dbReference type="eggNOG" id="arCOG05892">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2893232_0_0_2"/>
    <dbReference type="InParanoid" id="Q981A3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="66" mass="7525" checksum="354E5AB3B718F654" modified="2001-10-01" version="1">MMNMVSFFKLLGIGYILAIALLVWELTEETLHAAATSYILPFTIGAFIGFISFFLFVYFAPEEEHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q981B3</accession>
    <name>Q981B3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SOJ protein (Soj)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">soj</name>
        <name evidence="2" type="ordered locus">SSO0034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4 5 6" key="2">
        <citation type="journal article" date="2021" name="Nucleic Acids Res." volume="49" first="13150" last="13164">
            <title>Chromosome segregation in Archaea: SegA-and SegB-DNA complex structures provide insights into segrosome assembly.</title>
            <authorList>
                <person name="Yen C.Y."/>
                <person name="Lin M.G."/>
                <person name="Chen B.W."/>
                <person name="Ng I.W."/>
                <person name="Read N."/>
                <person name="Kabli A.F."/>
                <person name="Wu C.T."/>
                <person name="Shen Y.Y."/>
                <person name="Chen C.H."/>
                <person name="Barilla D."/>
                <person name="Sun Y.J."/>
                <person name="Hsiao C.D."/>
            </authorList>
            <dbReference type="PubMed" id="34850144"/>
            <dbReference type="DOI" id="10.1093/nar/gkab1155"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH ADP AND MG(2+)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90142">
        <property type="entry name" value="H90142"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989606.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="7DUT">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A=1-220"/>
    </dbReference>
    <dbReference type="PDB" id="7DV3">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A/B=1-220"/>
    </dbReference>
    <dbReference type="PDB" id="7DWR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B/C/D=1-220"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981B3"/>
    <dbReference type="SMR" id="Q981B3"/>
    <dbReference type="STRING" id="273057.SSO0034"/>
    <dbReference type="PaxDb" id="273057-SSO0034"/>
    <dbReference type="EnsemblBacteria" id="AAK40399">
        <property type="protein sequence ID" value="AAK40399"/>
        <property type="gene ID" value="SSO0034"/>
    </dbReference>
    <dbReference type="GeneID" id="1455290"/>
    <dbReference type="KEGG" id="sso:SSO0034"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.35"/>
    <dbReference type="eggNOG" id="arCOG00586">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1259116_0_0_2"/>
    <dbReference type="InParanoid" id="Q981B3"/>
    <dbReference type="PhylomeDB" id="Q981B3"/>
    <dbReference type="BRENDA" id="3.6.4.B11">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02042">
        <property type="entry name" value="ParAB_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050678">
        <property type="entry name" value="DNA_Partitioning_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13696:SF99">
        <property type="entry name" value="COBYRINIC ACID AC-DIAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13696">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4 5 6" id="KW-0002">3D-structure</keyword>
    <keyword evidence="4 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="2"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="7DUT"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="7DV3"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="7DWR"/>
        </source>
    </evidence>
    <sequence length="220" mass="24293" checksum="87277FACB527E872" modified="2001-10-01" version="1">MIVTVINQKGGVGKTTTSVNLSYYLSKEKKTGLLDLDPEGGATISYGMKRELKELPLGEKSVNIFNVEVFPAHIGLLKLELNGDVEEISNKIKEIGKQFDFLVIDTPPNLGTLAISAMLVADRIVSPVTPQPLALEAIKNLDSRLKSIGKNAYSFTNFSKKVVKLDNLSSVKFTEITIPPSRLFIEASRLGVPALRYEEVRIKKPKLANYYQQLAKVISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q981E1</accession>
    <name>Q981E1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90139">
        <property type="entry name" value="D90139"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989647.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981E1"/>
    <dbReference type="STRING" id="273057.SSO0006"/>
    <dbReference type="PaxDb" id="273057-SSO0006"/>
    <dbReference type="EnsemblBacteria" id="AAK40371">
        <property type="protein sequence ID" value="AAK40371"/>
        <property type="gene ID" value="SSO0006"/>
    </dbReference>
    <dbReference type="GeneID" id="1455261"/>
    <dbReference type="KEGG" id="sso:SSO0006"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.6"/>
    <dbReference type="eggNOG" id="arCOG00500">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031317_3_2_2"/>
    <dbReference type="InParanoid" id="Q981E1"/>
    <dbReference type="PhylomeDB" id="Q981E1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018:SF2">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="228" mass="25533" checksum="74696B055387FADE" modified="2001-10-01" version="1">MKITFIGTGAGSSLGLKRVKSSILINDKVLLDLGPGAELRLEDLRIHPKALFITHLHIDHFSGVFDYLVQRKIRQIPELVIYSPKGFSEVLNIYTRIGNNISAKIYESDLPSGKIDEMEIYSIQACHSIYAVSYIISDGNTRVLYTGDTKEPCNAILENIKDVDLIIHETTCVDDCSIWGHTSIKQIFELFSNKRIFATHIPAEIEEKIISLANNKINIAFDGLSLNV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UWT5</accession>
    <name>GLYA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="1">SHMT</shortName>
            <shortName evidence="1">Serine methylase</shortName>
            <ecNumber evidence="1">2.1.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glyA</name>
        <name type="ordered locus">SSO0530</name>
        <name type="ORF">C22_021</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1997" name="J. Bacteriol." volume="179" first="7456" last="7461">
            <title>Purification and properties of serine hydroxymethyltransferase from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Delle Fratte S."/>
                <person name="White R.H."/>
                <person name="Maras B."/>
                <person name="Bossa F."/>
                <person name="Schirch V."/>
            </authorList>
            <dbReference type="PubMed" id="9393711"/>
        </citation>
        <scope>PARTIAL PROTEIN SEQUENCE</scope>
        <scope>FUNCTION</scope>
        <scope>MODIFIED FOLATE-DEPENDENT SERINE HYDROXYMETHYLTRANSFERASE ACTIVITY</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>ALDOLASE ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible interconversion of serine and glycine with the modified folate sulfopterin serving as the one-carbon carrier. Cannot use tetrahydrofolate (THF or H4PteGlu) as the pteridine substrate. Also exhibits a pteridine-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. Thus, is able to catalyze the cleavage of both allo-threonine and beta-phenylserine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5,10-methylenetetrahydrosulfopterin + glycine + H2O = tetrahydrosulfopterin + L-serine</text>
            <dbReference type="Rhea" id="RHEA:56608"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:140605"/>
            <dbReference type="ChEBI" id="CHEBI:140606"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Is completely inhibited by addition of NaCNBH(3) in vitro; this reagent is a known inhibitor of PLP enzymes, that reduces the internal aldimine of PLP to the catalytically inactive and stable secondary amine. Is also inhibited by L-cysteine, which forms a thiazolidine complex with the active site PLP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.-" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90199">
        <property type="entry name" value="C90199"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991046.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UWT5"/>
    <dbReference type="SMR" id="Q9UWT5"/>
    <dbReference type="FunCoup" id="Q9UWT5">
        <property type="interactions" value="473"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0530"/>
    <dbReference type="PaxDb" id="273057-SSO0530"/>
    <dbReference type="EnsemblBacteria" id="AAK40850">
        <property type="protein sequence ID" value="AAK40850"/>
        <property type="gene ID" value="SSO0530"/>
    </dbReference>
    <dbReference type="GeneID" id="1454817"/>
    <dbReference type="GeneID" id="44129548"/>
    <dbReference type="KEGG" id="sso:SSO0530"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.529"/>
    <dbReference type="eggNOG" id="arCOG00070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_2_1_2"/>
    <dbReference type="InParanoid" id="Q9UWT5"/>
    <dbReference type="PhylomeDB" id="Q9UWT5"/>
    <dbReference type="BRENDA" id="2.1.2.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046653">
        <property type="term" value="P:tetrahydrofolate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000101">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000136">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF35">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000113725" description="Serine hydroxymethyltransferase">
        <location>
            <begin position="1"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="121"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="site" description="Plays an important role in substrate specificity" evidence="1">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="9393711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="9393711"/>
        </source>
    </evidence>
    <sequence length="433" mass="48509" checksum="8C239FA0FA3A285E" modified="2000-05-01" version="1">MSLPKELEKVLEITKAQNVWRRTQTLNLIASENVMSPLAESVYMSDFMSRYAEGKPYKRYYQGTKYTDEIETLTMELMNEITNSKDCDLRPTSGTIANAAVFRVLAEPGDKALIAPVQAGAHVSHTKFGTLGALGIQHIEMPFDEENINVDVDKAIKMIEEVKPKFVVLGGSLYLFPHPTKELAQHVHAVGAKLVYDAAHVYGLIEGKVWSNPLKDGADIMTVSTHKTFPGPQGGAIFSDGSEVFKQVSKTIFPWFVSNHHLHRLPATAVTAIEMKYFGESYANQILRNSKALAEALAERGFKVIGENLGYTKSHQVAVDVRQFGGGNKIAKLLEDANIIVNKNLLPYDKPEDVSDPSGLRIGVQEMTRYGMKEGEMEEIAELFKKVIIDKKDVNEVKKEVIEMRRNFLEVKYTFDDMKDLEKYSSKSLKLII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX90</accession>
    <name>RL32_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL32</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>50S ribosomal protein L32e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rpl32e</name>
        <name type="ordered locus">SSO0701</name>
        <name type="ORF">C10_029</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90218">
        <property type="entry name" value="C90218"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991259.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX90"/>
    <dbReference type="SMR" id="Q9UX90"/>
    <dbReference type="FunCoup" id="Q9UX90">
        <property type="interactions" value="239"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0701"/>
    <dbReference type="PaxDb" id="273057-SSO0701"/>
    <dbReference type="EnsemblBacteria" id="AAK41002">
        <property type="protein sequence ID" value="AAK41002"/>
        <property type="gene ID" value="SSO0701"/>
    </dbReference>
    <dbReference type="GeneID" id="1454969"/>
    <dbReference type="KEGG" id="sso:SSO0701"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.701"/>
    <dbReference type="eggNOG" id="arCOG00781">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071479_3_0_2"/>
    <dbReference type="InParanoid" id="Q9UX90"/>
    <dbReference type="PhylomeDB" id="Q9UX90"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00810">
        <property type="entry name" value="Ribosomal_eL32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023654">
        <property type="entry name" value="Ribosomal_eL32_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018263">
        <property type="entry name" value="Ribosomal_eL32_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006332">
        <property type="entry name" value="PRK08562.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00580">
        <property type="entry name" value="RIBOSOMAL_L32E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000131165" description="Large ribosomal subunit protein eL32">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="138" mass="16168" checksum="B929E6C4FEC39A52" modified="2000-05-01" version="1">MTEGRIQSYRKKIYVIRQKLKAKKPKFLRYDSDKFYRLGRQEKWRRPYGRDNKTRLKVRGFPAIVSVGYRLPKKVRGFHPSGLRQVIVHNVNELVKVQNQKDNVIVTISSSVGFKKRLEILNKARELGLKVSNEGVVT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1995-02-01" modified="2026-01-28" version="164" xmlns="https://uniprot.org/uniprot">
    <accession>P39462</accession>
    <accession>O74076</accession>
    <name>ADH_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>NAD-dependent alcohol dehydrogenase</fullName>
            <ecNumber>1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">adh</name>
        <name type="ordered locus">SSO2536</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1992" name="Biochemistry" volume="31" first="12514" last="12523">
            <title>Thermostable NAD(+)-dependent alcohol dehydrogenase from Sulfolobus solfataricus: gene and protein sequence determination and relationship to other alcohol dehydrogenases.</title>
            <authorList>
                <person name="Ammendola S."/>
                <person name="Raia C.A."/>
                <person name="Caruso C."/>
                <person name="Camardella L."/>
                <person name="D'Auria S."/>
                <person name="de Rosa M."/>
                <person name="Rossi M."/>
            </authorList>
            <dbReference type="PubMed" id="1463738"/>
            <dbReference type="DOI" id="10.1021/bi00164a031"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>METHYLATION AT LYS-11 AND LYS-213</scope>
        <scope>PARTIAL PROTEIN SEQUENCE</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="1998-08" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Aravalli R.N."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="journal article" date="2002" name="J. Mol. Biol." volume="318" first="463" last="477">
            <title>Crystal structure of the alcohol dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus at 1.85 A resolution.</title>
            <authorList>
                <person name="Esposito L."/>
                <person name="Sica F."/>
                <person name="Raia C.A."/>
                <person name="Giordano A."/>
                <person name="Rossi M."/>
                <person name="Mazzarella L."/>
                <person name="Zagari A."/>
            </authorList>
            <dbReference type="PubMed" id="12051852"/>
            <dbReference type="DOI" id="10.1016/S0022-2836(02)00088-8"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH ZN(2+)</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a secondary alcohol + NAD(+) = a ketone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1"/>
    <dbReference type="EMBL" id="S51211">
        <property type="protein sequence ID" value="AAB24546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AJ010590">
        <property type="protein sequence ID" value="CAA09258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="AAK42665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="PIR" id="A44245">
        <property type="entry name" value="A44245"/>
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    <dbReference type="RefSeq" id="WP_009990984.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="PDB" id="1JVB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.85 A"/>
        <property type="chains" value="A=1-347"/>
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    <dbReference type="PDB" id="1NTO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.94 A"/>
        <property type="chains" value="A/B/C/D/E/H=1-347"/>
    </dbReference>
    <dbReference type="PDB" id="1NVG">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A=1-347"/>
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    <dbReference type="PDB" id="1R37">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B=1-347"/>
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    <dbReference type="PDB" id="3I4C">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B/C/D/E/H=1-347"/>
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    <dbReference type="PDBsum" id="1JVB"/>
    <dbReference type="PDBsum" id="1NTO"/>
    <dbReference type="PDBsum" id="1NVG"/>
    <dbReference type="PDBsum" id="1R37"/>
    <dbReference type="PDBsum" id="3I4C"/>
    <dbReference type="AlphaFoldDB" id="P39462"/>
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    <dbReference type="FunCoup" id="P39462">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2536"/>
    <dbReference type="iPTMnet" id="P39462"/>
    <dbReference type="PaxDb" id="273057-SSO2536"/>
    <dbReference type="EnsemblBacteria" id="AAK42665">
        <property type="protein sequence ID" value="AAK42665"/>
        <property type="gene ID" value="SSO2536"/>
    </dbReference>
    <dbReference type="GeneID" id="1453992"/>
    <dbReference type="KEGG" id="sso:SSO2536"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2612"/>
    <dbReference type="eggNOG" id="arCOG01455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_2_2"/>
    <dbReference type="InParanoid" id="P39462"/>
    <dbReference type="PhylomeDB" id="P39462"/>
    <dbReference type="BRENDA" id="1.1.1.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="P39462"/>
    <dbReference type="EvolutionaryTrace" id="P39462"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05284">
        <property type="entry name" value="arabinose_DH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000160753" description="NAD-dependent alcohol dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="10"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2 5">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 5">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 5">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 5">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 5">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 5">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 5">
        <location>
            <position position="112"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 5">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
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            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-methyllysine; partial" evidence="3">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine; partial" evidence="3">
        <location>
            <position position="213"/>
        </location>
    </feature>
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        <location>
            <begin position="2"/>
            <end position="6"/>
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    <feature type="strand" evidence="7">
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            <begin position="8"/>
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            <begin position="14"/>
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        </location>
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    <feature type="strand" evidence="6">
        <location>
            <begin position="28"/>
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    <feature type="helix" evidence="6">
        <location>
            <begin position="40"/>
            <end position="44"/>
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            <begin position="45"/>
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            <begin position="49"/>
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            <begin position="54"/>
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            <begin position="62"/>
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    <feature type="strand" evidence="6">
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    <feature type="strand" evidence="6">
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            <end position="92"/>
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    <feature type="strand" evidence="6">
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    <feature type="helix" evidence="6">
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    <evidence type="ECO:0000255" key="1"/>
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            <dbReference type="PubMed" id="12051852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
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            <dbReference type="PubMed" id="1463738"/>
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    <evidence type="ECO:0000305" key="4"/>
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    <evidence type="ECO:0007829" key="7">
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    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="1NVG"/>
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    </evidence>
    <sequence length="347" mass="37569" checksum="755848A249D4F4A2" modified="1995-02-01" version="1">MRAVRLVEIGKPLSLQEIGVPKPKGPQVLIKVEAAGVCHSDVHMRQGRFGNLRIVEDLGVKLPVTLGHEIAGKIEEVGDEVVGYSKGDLVAVNPWQGEGNCYYCRIGEEHLCDSPRWLGINFDGAYAEYVIVPHYKYMYKLRRLNAVEAAPLTCSGITTYRAVRKASLDPTKTLLVVGAGGGLGTMAVQIAKAVSGATIIGVDVREEAVEAAKRAGADYVINASMQDPLAEIRRITESKGVDAVIDLNNSEKTLSVYPKALAKQGKYVMVGLFGADLHYHAPLITLSEIQFVGSLVGNQSDFLGIMRLAEAGKVKPMITKTMKLEEANEAIDNLENFKAIGRQVLIP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>P58156</accession>
    <name>CBPX2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Thermostable carboxypeptidase 2</fullName>
            <ecNumber>3.4.17.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cpsA2</name>
        <name type="synonym">cpsA-2</name>
        <name type="ordered locus">SSO1952</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Can release basic, acidic, aromatic, and, to a lesser extent, aliphatic amino acids.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M20 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.-"/>
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        <property type="protein sequence ID" value="AAK42144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="A99361"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58156"/>
    <dbReference type="SMR" id="P58156"/>
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        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1952"/>
    <dbReference type="MEROPS" id="M20.008"/>
    <dbReference type="PaxDb" id="273057-SSO1952"/>
    <dbReference type="EnsemblBacteria" id="AAK42144">
        <property type="protein sequence ID" value="AAK42144"/>
        <property type="gene ID" value="SSO1952"/>
    </dbReference>
    <dbReference type="GeneID" id="1453471"/>
    <dbReference type="KEGG" id="sso:SSO1952"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2026"/>
    <dbReference type="eggNOG" id="arCOG01108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023257_0_1_2"/>
    <dbReference type="InParanoid" id="P58156"/>
    <dbReference type="PhylomeDB" id="P58156"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08021">
        <property type="entry name" value="M20_Acy1_YhaA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000014">
        <property type="entry name" value="N-acyl-L-amino acid amidohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053493">
        <property type="entry name" value="Thermostable_CP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040868">
        <property type="entry name" value="carboxypep_CpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014:SF63">
        <property type="entry name" value="METALLOPEPTIDASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G09600)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PEPTIDASE M20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0121">Carboxypeptidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000061948" description="Thermostable carboxypeptidase 2">
        <location>
            <begin position="1"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="373"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="393" mass="42993" checksum="AFBDE9F0A3469B63" modified="2001-06-01" version="1">MDLVEKLKNDVKEIEDWIIQIRRKIHENPELSYKEYSTSKLVAETLRKLGIEVEEGVGLPTAVVGKIRGNKPGKTVALRADMDALPVEETSDVEFKSKVKGVMHACGHDTHVAMLLGGAYLLVKNKDLISGEIRLIFQPAEEDGGLGGAKPMIEAGVMNGVDYVFGIHISSSYPSGVFATRKGPIMATPDAFKIVVHGKGGHGSAPHETIDPIFISLQIANAIYGITARQIDPVQPFVISITTIHSGTKDNIIPDDAEMQGTIRSLDENVRSKAKDYMRRIVSSICGIYGATCEVKFMEDVYPITVNNPEVTDEVMKILSSISTVVETEPVLGAEDFSRFLQKAPGMYFFLGTRNEKKGCIYPNHSSKFCVDEDVLKLGALAHALLAIKFSNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2024-03-27" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>P95879</accession>
    <name>Y2105_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein SSO2105</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2105</name>
        <name type="ORF">C06032</name>
        <name type="ORF">C06_015</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To A.pernix APE1276 and APE1804.</text>
    </comment>
    <dbReference type="EMBL" id="Y08256">
        <property type="protein sequence ID" value="CAA69477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74017">
        <property type="entry name" value="S74017"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95879"/>
    <dbReference type="SMR" id="P95879"/>
    <dbReference type="STRING" id="273057.SSO2105"/>
    <dbReference type="PaxDb" id="273057-SSO2105"/>
    <dbReference type="EnsemblBacteria" id="AAK42284">
        <property type="protein sequence ID" value="AAK42284"/>
        <property type="gene ID" value="SSO2105"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2105"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2191"/>
    <dbReference type="eggNOG" id="arCOG04197">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064882_1_0_2"/>
    <dbReference type="InParanoid" id="P95879"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010094">
        <property type="entry name" value="Transposase_put_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01765">
        <property type="entry name" value="tspaseT_teng_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000216225" description="Uncharacterized protein SSO2105">
        <location>
            <begin position="1"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="369" mass="42640" checksum="599AE6C1834090A3" modified="1997-05-01" version="1">MLSYQMMRSKLIGSKEALDNFQFVTINGKVIFNEKDKVVRIARVYSQVVKSAIKPLFDGKSVDELTKEFYNVLPNYVYLETALKQAKTIVEGLLEREEERGEIIHARIRKFWFGSRGNKSDRGNRNMKFRVLEDRVLIKVRDPWNKEWIFGKAYFGKEYLPLLKELEDLAQRKEEGYGALVSFKEKSMIHLQIPLWLYLKHFSLPKPTGYGLIAGFDLNSDRLNVVVINKDGKVITTRTFWYSDVTRPGFPKVKARALRLNALSNSLEFLSRIGVDYVVFEDLFLVKRRKFIKSKSGNRKISKFAKKQLLIHGVIKSLRFGFNVVLVNPKGTTNSGEHDRVMREKGFDKHTASAYLIALKGLGMLNDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-03-27" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX51</accession>
    <name>Q7LX51_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2153</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90384">
        <property type="entry name" value="B90384"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923793.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX51"/>
    <dbReference type="PaxDb" id="273057-SSO2153"/>
    <dbReference type="EnsemblBacteria" id="AAK42329">
        <property type="protein sequence ID" value="AAK42329"/>
        <property type="gene ID" value="SSO2153"/>
    </dbReference>
    <dbReference type="GeneID" id="1453656"/>
    <dbReference type="KEGG" id="sso:SSO2153"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2246"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077283_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX51"/>
    <dbReference type="PhylomeDB" id="Q7LX51"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="354" mass="40290" checksum="876FF4C11EA1A682" modified="2011-05-31" version="1">MTKELTREEYYKALEKAVNEVILSMTGTRKDVAKRLLLGAVVGRNATEIAQEAEMDYETVLNNLDKAAQAKLIEVVKKLVGDHPVLLIIDDTHDHKLYARAMPVSRNGAQIFYCRAHKRFEPAIQLLVIGVKDLVNNQIYVIHIIAYIPRKVEEELKRRGEEVKFKTKIDALLEFLSSLSGLNVKARVFDSWYVNSRTLQGNTVGELKSSARVVEGGRSVPVSEFPQGEYLVEYLGTPIKLLVIDDYKGYGRRYFFSTDLNDTAEDIITTWENRWDIEVLIRELKALGLEGGSFLTWVRNSGFVALKALSLLVVQYFKYSTGLMLGAKRLARLIKSIYREAGGIKKLFKRRRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-03-27" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX60</accession>
    <name>Q7LX60_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74054">
        <property type="entry name" value="S74054"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX60"/>
    <dbReference type="PaxDb" id="273057-SSO2142"/>
    <dbReference type="EnsemblBacteria" id="AAK42320">
        <property type="protein sequence ID" value="AAK42320"/>
        <property type="gene ID" value="SSO2142"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2142"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2236"/>
    <dbReference type="HOGENOM" id="CLU_2191146_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX60"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="109" mass="13057" checksum="74A9307CC37B38DE" modified="2011-05-31" version="1">MMRFLSKIIDIKSSLHSIVIMENKNTLKEVEDFLRAKTNIFKVRSYVYEGIIKVDEYIYKDLKIELFYCDEKLRHIIIKSSDLNKTFNNDESTQNIAEIIEYIEDKLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX84</accession>
    <name>Q7LX84_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2112</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74024">
        <property type="entry name" value="S74024"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990142.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX84"/>
    <dbReference type="PaxDb" id="273057-SSO2112"/>
    <dbReference type="EnsemblBacteria" id="AAK42291">
        <property type="protein sequence ID" value="AAK42291"/>
        <property type="gene ID" value="SSO2112"/>
    </dbReference>
    <dbReference type="GeneID" id="1453618"/>
    <dbReference type="KEGG" id="sso:SSO2112"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2200"/>
    <dbReference type="eggNOG" id="arCOG01931">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044590_4_2_2"/>
    <dbReference type="InParanoid" id="Q7LX84"/>
    <dbReference type="PhylomeDB" id="Q7LX84"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01292">
        <property type="entry name" value="metallo-dependent_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000089">
        <property type="entry name" value="Amidohydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="2-AMINO-3-CARBOXYLMUCONATE-6-SEMIALDEHYDE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240:SF19">
        <property type="entry name" value="CATALYTIC_ HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="2">
        <location>
            <begin position="2"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="269" mass="31017" checksum="9C3039E2BF670DE4" modified="2011-05-31" version="1">MIDFHFHAPVKEFLDFLGEFGEPAIKYFNAKVEVKGLKETLDYYESFGIKRFVVLPIDSTTFLGRRIPNQVINLDDRIVKFISVDPSKPNADEELKRSIKEFEPVGVKLHPQLQGFHPLDERALKIYEIIDNHGLIVVFHTGTSGIGAGVKSSIRLDYGRPIYFDEIAVRFQNMKIILAHFGWPWTEEAIAIALHKPNVYLDLSGWAPRYIPQVIWSNAKRLSDKLLFGSDFPLIRPERWLEEFKSVNLSQDIKDKILKHNAEKLVKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXE0</accession>
    <name>Q7LXE0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thermopsin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90234">
        <property type="entry name" value="F90234"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923128.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXE0"/>
    <dbReference type="PaxDb" id="273057-SSO0840"/>
    <dbReference type="EnsemblBacteria" id="AAK41133">
        <property type="protein sequence ID" value="AAK41133"/>
        <property type="gene ID" value="SSO0840"/>
    </dbReference>
    <dbReference type="GeneID" id="1455100"/>
    <dbReference type="KEGG" id="sso:SSO0840"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.853"/>
    <dbReference type="eggNOG" id="arCOG07246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074747_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXE0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="324" mass="35514" checksum="FC16AEF5BA2D37A2" modified="2011-05-31" version="1">MRLLLLLLMLTITLLSSVSSFTASVSVQYPKEVILGSKISINFSLTQQEINSTAFPFITAGVREISEEPLILEGAGIAGSFAVFKINNSSQVVVITFIGKDNTSPGWNPGIVVYGGNFNPHVSDLSQGDFTAVLITFTGRLWVHTPSKGWFTLSCSLPSIAPQRDGWINATKPFNYTAILEDVNGSIFVNYVILNGEKYIVDYQTPIPWNFTYVGVRIDPSTVTVCGFYVTIPSPHQPYVVYVNGKEYTKGYTNSLGEGSVSLTISSPSMVVNISFPSAHVFRVITISAQRDANIHVEYPILQYALLGVSVVLVAISIIWRKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXG3</accession>
    <name>Q7LXG3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO0813</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDPGP type 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90231">
        <property type="entry name" value="G90231"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXG3"/>
    <dbReference type="FunCoup" id="Q7LXG3">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0813"/>
    <dbReference type="PaxDb" id="273057-SSO0813"/>
    <dbReference type="EnsemblBacteria" id="AAK41110">
        <property type="protein sequence ID" value="AAK41110"/>
        <property type="gene ID" value="SSO0813"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0813"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.824"/>
    <dbReference type="eggNOG" id="arCOG00665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_1_2_2"/>
    <dbReference type="InParanoid" id="Q7LXG3"/>
    <dbReference type="PhylomeDB" id="Q7LXG3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005771">
        <property type="entry name" value="GalU_uridylyltTrfase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197">
        <property type="entry name" value="UTP--GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197:SF1">
        <property type="entry name" value="UTP--GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="246" mass="28387" checksum="9D3E91436C2E63E8" modified="2011-05-31" version="1">MGTRLLPASKEIPKEMFPIPFNGQFKPIIQIIFEQLYDIGIRDFVIVVGRGKRVIEDHFTPDYDFINYLEAKGKVIQANYLRQFYQKVENSRIAFVNQPEPKGFGDAVLRAEPFIQGEFLVVAADTILPMIPISSMVVNSFLVTTVQDPRPYGVVLVDKQGCVIDIEEKPKQPKSNLVVVPYYMFDEEIFDELRQIKYEEELQLTDGIKGLIKRGRRFKAIMVDKVYDLGTVENYIESLRRLLNND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXH0</accession>
    <name>Q7LXH0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Partial transposase in ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0803</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90230">
        <property type="entry name" value="F90230"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXH0"/>
    <dbReference type="PaxDb" id="273057-SSO0803"/>
    <dbReference type="EnsemblBacteria" id="AAK41101">
        <property type="protein sequence ID" value="AAK41101"/>
        <property type="gene ID" value="SSO0803"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0803"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2204233_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXH0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="2">
        <location>
            <begin position="23"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="107" mass="11779" checksum="A292F56D2AF41ADC" modified="2011-05-31" version="1">MMLGVLMIRGSLWLIVVLILLLSGGSVVSRGVSRRGDAVLRRAFYLAALTAIKVNPVIKRFYEEHKGRLKGKKLIVACAMLLLLGLCCIIISHLMLTGDDEGFCLHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXL8</accession>
    <name>Q7LXL8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF447 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0724</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90220">
        <property type="entry name" value="G90220"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991288.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXL8"/>
    <dbReference type="STRING" id="273057.SSO0724"/>
    <dbReference type="PaxDb" id="273057-SSO0724"/>
    <dbReference type="EnsemblBacteria" id="AAK41022">
        <property type="protein sequence ID" value="AAK41022"/>
        <property type="gene ID" value="SSO0724"/>
    </dbReference>
    <dbReference type="GeneID" id="1454989"/>
    <dbReference type="KEGG" id="sso:SSO0724"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.722"/>
    <dbReference type="eggNOG" id="arCOG04458">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1406014_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXL8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.290">
        <property type="entry name" value="Hypothetical membrane protein ta0354_69_121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049288">
        <property type="entry name" value="DUF447_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007386">
        <property type="entry name" value="DUF447_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016733">
        <property type="entry name" value="UCP018747"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20766">
        <property type="entry name" value="DUF447_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04289">
        <property type="entry name" value="DUF447_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018747">
        <property type="entry name" value="UCP018747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF447" evidence="1">
        <location>
            <begin position="15"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="DUF447" evidence="2">
        <location>
            <begin position="131"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="22537" checksum="5C288366DA2FB8F0" modified="2011-05-31" version="1">MKKFLKMFFPHDGIYEVLVGTSGIKPIIKPLGIIVEANELRSKIYKSTLTYSNLEKNNKCSIHVTLDTTFFILSMLDKLTFSIDSDYNVPVLKNLNVIYAECMKTSSTDPSIFTINPLDLEINSNLTRAYNRGDYISVDFLVNYTRLDIYKGEDLEKLVRILEYEWSVIKRTSQYTSSLLEEILNKMRSKGFKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXP4</accession>
    <name>Q7LXP4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Biotin--(Acetyl-CoA carboxylase) synthetase (BioR)</fullName>
            <ecNumber evidence="3">6.3.4.15</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bioR</name>
        <name evidence="3" type="ordered locus">SSO0662</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.3.4.15" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90213">
        <property type="entry name" value="H90213"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXP4"/>
    <dbReference type="STRING" id="273057.SSO0662"/>
    <dbReference type="PaxDb" id="273057-SSO0662"/>
    <dbReference type="EnsemblBacteria" id="AAK40967">
        <property type="protein sequence ID" value="AAK40967"/>
        <property type="gene ID" value="SSO0662"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0662"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.664"/>
    <dbReference type="eggNOG" id="arCOG01940">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051096_3_0_2"/>
    <dbReference type="InParanoid" id="Q7LXP4"/>
    <dbReference type="PhylomeDB" id="Q7LXP4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="233" mass="26757" checksum="BE826F8C4050049F" modified="2011-05-31" version="1">MCGSMLVIRLNKVTSTQDFAEAISNMLFEDFLVIAEEQTKARGRLGRSWYSPKGGLWFTYVKKNFKTEEIQLSSLKVAVAVLDVLRKFVDAKIRWPNDIVVNDKKIAGILIEAKTYEEDIKLADLFIGAGIDILIKEFPSDLNATSLYLEIGKEVELNIDEVIGKIEEHLKLDNRKIIDIVNQYLSIKDREVTLKGENWEKRCKALFVDYMGRLVTECGIYEVEEIYRVEVSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXU5</accession>
    <name>Q7LXU5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ATP synthase subunit K (AtpK)</fullName>
            <ecNumber evidence="10">3.6.3.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">atpK</name>
        <name evidence="10" type="ordered locus">SSO0567</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.14" evidence="10"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90203">
        <property type="entry name" value="D90203"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991080.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXU5"/>
    <dbReference type="FunCoup" id="Q7LXU5">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0567"/>
    <dbReference type="PaxDb" id="273057-SSO0567"/>
    <dbReference type="EnsemblBacteria" id="AAK40883">
        <property type="protein sequence ID" value="AAK40883"/>
        <property type="gene ID" value="SSO0567"/>
    </dbReference>
    <dbReference type="GeneID" id="1454850"/>
    <dbReference type="KEGG" id="sso:SSO0567"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.576"/>
    <dbReference type="eggNOG" id="arCOG02455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148047_3_1_2"/>
    <dbReference type="InParanoid" id="Q7LXU5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18120">
        <property type="entry name" value="ATP-synt_Vo_Ao_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004888">
        <property type="entry name" value="PRK06251.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="9">
        <location>
            <begin position="38"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK40883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="102" mass="10094" checksum="A30F3F3EA03180BF" modified="2011-05-31" version="1">MKRASLILAFLIPVLVSSVIAAAQAPSDTAQGFAGINIGAGLAVGLAAIGAGIAVGMAAAAGVGVLTERRDMFGTVLIFVAIGEGIAVYGILFAVLMLFGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LYL6</accession>
    <name>Q7LYL6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="4 9">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">SSO0758</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4 9"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90224">
        <property type="entry name" value="G90224"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991339.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LYL6"/>
    <dbReference type="STRING" id="273057.SSO0758"/>
    <dbReference type="PaxDb" id="273057-SSO0758"/>
    <dbReference type="EnsemblBacteria" id="AAK41054">
        <property type="protein sequence ID" value="AAK41054"/>
        <property type="gene ID" value="SSO0758"/>
    </dbReference>
    <dbReference type="GeneID" id="1455021"/>
    <dbReference type="KEGG" id="sso:SSO0758"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.756"/>
    <dbReference type="eggNOG" id="arCOG00980">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073526_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LYL6"/>
    <dbReference type="PhylomeDB" id="Q7LYL6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054016">
        <property type="entry name" value="FKBP26_IF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048261">
        <property type="entry name" value="SlpA/SlyD-like_ins_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861:SF3">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22199">
        <property type="entry name" value="FKBP26_IF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="10">
        <location>
            <begin position="5"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK41054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="235" mass="26717" checksum="57A82A9E6A61F9B2" modified="2011-05-31" version="1">MFKNNDFIYIEYTAKIKDTGEIVDTTNEEEAKKANIYDETRNYGPRLVILGENRLIKGLEEALYNFNLNEEKTIEIPPEKAYGERDNSKIKIVPLGELRRQGITAVPNRVLRLSDGSLAVIRSVSGGRVVLDLNHPLAGRTLVYNVKVVKVLNNAKDKIMALIERRFSSKYANGFNVELDDGKKSVRITIPKDLYLVEDLQINVYSLAYEIVNYVLADYTVEVLQQYNKSTFSSS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2026-01-28" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>Q7SI94</accession>
    <accession>Q980W6</accession>
    <name>LYSJ_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">[LysW]-aminoadipate semialdehyde/glutamate semialdehyde transaminase</fullName>
            <ecNumber evidence="1">2.6.1.118</ecNumber>
            <ecNumber evidence="1">2.6.1.124</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lysJ</name>
        <name type="ordered locus">SSO0160</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="29537" last="29549">
            <title>The Sulfolobus solfataricus Lrp-like protein LysM regulates lysine biosynthesis in response to lysine availability.</title>
            <authorList>
                <person name="Brinkman A.B."/>
                <person name="Bell S.D."/>
                <person name="Lebbink R.J."/>
                <person name="de Vos W.M."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="12042311"/>
            <dbReference type="DOI" id="10.1074/jbc.M203528200"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in both the arginine and lysine biosynthetic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-lysyl)-L-glutamate + 2-oxoglutarate = [amino-group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:41952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9714"/>
            <dbReference type="Rhea" id="RHEA-COMP:9715"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:78501"/>
            <dbReference type="ChEBI" id="CHEBI:78526"/>
            <dbReference type="EC" id="2.6.1.118"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-ornithyl)-L-glutamate + 2-oxoglutarate = [amino-group carrier protein]-C-terminal-gamma-(L-glutamyl-5-semialdehyde)-L-glutamate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:52672"/>
            <dbReference type="Rhea" id="RHEA-COMP:13327"/>
            <dbReference type="Rhea" id="RHEA-COMP:13328"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:136761"/>
            <dbReference type="ChEBI" id="CHEBI:136763"/>
            <dbReference type="EC" id="2.6.1.124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="1">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 4/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. LysJ subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40506" version="1"/>
        </conflict>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="DAA00054" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.6.1.118" evidence="1"/>
    <dbReference type="EC" id="2.6.1.124" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40506.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BK000545">
        <property type="protein sequence ID" value="DAA00054.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90156">
        <property type="entry name" value="C90156"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990385.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7SI94"/>
    <dbReference type="SMR" id="Q7SI94"/>
    <dbReference type="FunCoup" id="Q7SI94">
        <property type="interactions" value="275"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0160"/>
    <dbReference type="PaxDb" id="273057-SSO0160"/>
    <dbReference type="EnsemblBacteria" id="AAK40506">
        <property type="protein sequence ID" value="AAK40506"/>
        <property type="gene ID" value="SSO0160"/>
    </dbReference>
    <dbReference type="GeneID" id="1453333"/>
    <dbReference type="GeneID" id="44129122"/>
    <dbReference type="KEGG" id="sso:SSO0160"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.157"/>
    <dbReference type="eggNOG" id="arCOG00914">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_0_2"/>
    <dbReference type="InParanoid" id="Q7SI94"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00038"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000004">
        <property type="entry name" value="Acetylornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02084">
        <property type="entry name" value="LysJ_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053458">
        <property type="entry name" value="Class-III_PLP-Dep_Atrans_LysJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037537">
        <property type="entry name" value="LysJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045491">
        <property type="entry name" value="LysJ_Sulfobales"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF79">
        <property type="entry name" value="ACETYLORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000112830" description="[LysW]-aminoadipate semialdehyde/glutamate semialdehyde transaminase">
        <location>
            <begin position="1"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="208"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="388" mass="43323" checksum="E0A2368FDB6E80F2" modified="2009-05-05" version="2">MIKLLKFYQDRGIKIIKGEGQYVWDEKNNKYLDMHAGHGVAFLGHRNKVIIDHLKKQMEEISTLSLAFDTPIREEMIKELDELKPEDLDNLFLLNSGSEAVELALKIARKITKRRKIVAFKNSFHGRSMGALSVTWNKKYREPFEPLIGPVEFLEYNNVDSLKSITEDTAAVIVEPVQGEGGVIPAKKEFVKSLREVTEKVNALLIIDEVQTGFGRTGKIWAYQHFDIKPDILTAGKAIGGGFPVSAVFLPNWISEKIEEGDHGSTYGGNPLAAAAVTAACKVAKSEKIAEQAQKKGELFMRILKEKLEDFKIVREIRGLGLMIGIDLKVNPSIAIKVLQDEKVLSLKAGLTTIRFLPPYLITQSDMEWASDATRKGISETESKRVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-10-02" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TW2</accession>
    <name>Q97TW2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">First ORF in transposon ISC1048</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1719</name>
        <name evidence="3" type="ordered locus">SSO2927</name>
        <name evidence="4" type="ordered locus">SSO2949</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90333">
        <property type="entry name" value="B90333"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TW2"/>
    <dbReference type="STRING" id="273057.SSO1719"/>
    <dbReference type="PaxDb" id="273057-SSO1719"/>
    <dbReference type="EnsemblBacteria" id="AAK41921">
        <property type="protein sequence ID" value="AAK41921"/>
        <property type="gene ID" value="SSO1719"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK43033">
        <property type="protein sequence ID" value="AAK43033"/>
        <property type="gene ID" value="SSO2927"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK43051">
        <property type="protein sequence ID" value="AAK43051"/>
        <property type="gene ID" value="SSO2949"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1719"/>
    <dbReference type="KEGG" id="sso:SSO2927"/>
    <dbReference type="KEGG" id="sso:SSO2949"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1765"/>
    <dbReference type="eggNOG" id="arCOG02130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124929_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025959">
        <property type="entry name" value="Winged_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13551">
        <property type="entry name" value="HTH_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13592">
        <property type="entry name" value="HTH_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Winged helix-turn helix" evidence="1">
        <location>
            <begin position="94"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="150" mass="17821" checksum="588E7ABC615D69B7" modified="2001-10-01" version="1">MQDLVQAYKEEKNARIKERILAVKLHVVDGKSEKEVSKMLNKGYSTIKLWIGKYKKEGLDGLKDKPRSGRPRKVEEEKIKQILEDKPQKYGIQQEYWTMKTLKIALQEQGIEYKKSRLYELVHELGYNLVKPRPTNIQAEKDKWEDFKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TW4</accession>
    <name>Q97TW4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0033 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO7239</name>
        <name evidence="4" type="ordered locus">SSO8861</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfur carrier protein TusA family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90337">
        <property type="entry name" value="B90337"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989065.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TW4"/>
    <dbReference type="FunCoup" id="Q97TW4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO7239"/>
    <dbReference type="PaxDb" id="273057-SSO7239"/>
    <dbReference type="EnsemblBacteria" id="AAK41381">
        <property type="protein sequence ID" value="AAK41381"/>
        <property type="gene ID" value="SSO7239"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41953">
        <property type="protein sequence ID" value="AAK41953"/>
        <property type="gene ID" value="SSO8861"/>
    </dbReference>
    <dbReference type="GeneID" id="1454732"/>
    <dbReference type="KEGG" id="sso:SSO7239"/>
    <dbReference type="KEGG" id="sso:SSO8861"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1121"/>
    <dbReference type="eggNOG" id="arCOG02062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165255_1_2_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00291">
        <property type="entry name" value="SirA_YedF_YeeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="entry name" value="TusA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279:SF6">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SirA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="2">
        <location>
            <begin position="11"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="80" mass="8783" checksum="347055F19731EB57" modified="2001-10-01" version="1">MSQEVRIAKTLDARGMYCPGPVLETAKAIKQINVGEVLEILATDPAAKPDIEAWARRTGNQIVDIQQQGGVTRILIKRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TY5</accession>
    <name>Q97TY5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90510">
        <property type="entry name" value="D90510"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989660.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TY5"/>
    <dbReference type="STRING" id="273057.SSO3247"/>
    <dbReference type="PaxDb" id="273057-SSO3247"/>
    <dbReference type="EnsemblBacteria" id="AAK43341">
        <property type="protein sequence ID" value="AAK43341"/>
        <property type="gene ID" value="SSO3247"/>
    </dbReference>
    <dbReference type="GeneID" id="1453258"/>
    <dbReference type="KEGG" id="sso:SSO3247"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3346"/>
    <dbReference type="eggNOG" id="arCOG05975">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153618_0_0_2"/>
    <dbReference type="InParanoid" id="Q97TY5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="117" mass="13119" checksum="C84652C723D73920" modified="2001-10-01" version="1">MKLIYFSLLLTAVSLLIGSIMLFNTVPRIFTIGTLAIVMFLIASLFLINKYNFLTYILFVLAILAIIISSSSGAHVQAFREFGESLYITALDILMILGFYVGPILYIVAFLKDNLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U16</accession>
    <name>Q97U16_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="4">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ilvB-6</name>
        <name evidence="9" type="ordered locus">SSO3210</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90506">
        <property type="entry name" value="C90506"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U16"/>
    <dbReference type="FunCoup" id="Q97U16">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3210"/>
    <dbReference type="PaxDb" id="273057-SSO3210"/>
    <dbReference type="EnsemblBacteria" id="AAK43306">
        <property type="protein sequence ID" value="AAK43306"/>
        <property type="gene ID" value="SSO3210"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3210"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3312"/>
    <dbReference type="eggNOG" id="arCOG03657">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_4_0_2"/>
    <dbReference type="InParanoid" id="Q97U16"/>
    <dbReference type="PhylomeDB" id="Q97U16"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="TPP_BFDC"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006203">
        <property type="entry name" value="PRK08327.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="8">
        <location>
            <begin position="7"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="7">
        <location>
            <begin position="389"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK43306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="543" mass="61833" checksum="18B4887DA6F55173" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U31</accession>
    <name>Q97U31_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sodium/calcium exchanger membrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO3192</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90504">
        <property type="entry name" value="D90504"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991692.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U31"/>
    <dbReference type="FunCoup" id="Q97U31">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3192"/>
    <dbReference type="PaxDb" id="273057-SSO3192"/>
    <dbReference type="DNASU" id="1453210"/>
    <dbReference type="EnsemblBacteria" id="AAK43291">
        <property type="protein sequence ID" value="AAK43291"/>
        <property type="gene ID" value="SSO3192"/>
    </dbReference>
    <dbReference type="GeneID" id="1453210"/>
    <dbReference type="KEGG" id="sso:SSO3192"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3297"/>
    <dbReference type="eggNOG" id="arCOG02881">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077877_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U31"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070588">
        <property type="term" value="P:calcium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="5"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="168"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="312" mass="34129" checksum="6E5273166CAA10E3" modified="2001-10-01" version="1">MVWFLILQLIVLLILVAFSATLMAKGTRELERSFGKGIAGGLILGFINALPETIIVIQAVLNGFYDIALGSALGGNILLLTLGIGLISIFYYLKYKSNIITLDSDINIEYSSFLLAVIILGIAIVYGKLNYYIGLFLLSPYIYYIYRRYKNYRGILKNEKNKGNAIKGLAYVMIGGLPLIFISKYLISTISSIASVFNVQPLILAILITPIAAELEENLTAIKLISDSPSSATTALMNFIGSKLENMTLLLSIIGISQTVSLRPSLLYLLLILATSLLALGIIKDRNIKVKEGLSLFGIYAILIAILLRFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U44</accession>
    <name>Q97U44_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO12127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90502">
        <property type="entry name" value="G90502"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U44"/>
    <dbReference type="PaxDb" id="273057-SSO12127"/>
    <dbReference type="EnsemblBacteria" id="AAK43278">
        <property type="protein sequence ID" value="AAK43278"/>
        <property type="gene ID" value="SSO12127"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO12127"/>
    <dbReference type="HOGENOM" id="CLU_2839544_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U44"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="65" mass="7675" checksum="9A9ECC7BC62809C4" modified="2001-10-01" version="1">MIYTLLLKLLSKFILLVKINFYSFTLCNMKNFLVRKKIFCNISLPNSELLHFLRNNIQALNPSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U77</accession>
    <name>Q97U77_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90498">
        <property type="entry name" value="E90498"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990907.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U77"/>
    <dbReference type="STRING" id="273057.SSO3146"/>
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    <dbReference type="EnsemblBacteria" id="AAK43244">
        <property type="protein sequence ID" value="AAK43244"/>
        <property type="gene ID" value="SSO3146"/>
    </dbReference>
    <dbReference type="GeneID" id="1453163"/>
    <dbReference type="KEGG" id="sso:SSO3146"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3250"/>
    <dbReference type="eggNOG" id="arCOG05930">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_1040590_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U77"/>
    <dbReference type="PhylomeDB" id="Q97U77"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049688">
        <property type="entry name" value="CedA_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041796">
        <property type="entry name" value="Ced_CedA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="262" mass="29138" checksum="DE89FF7321AD0BCF" modified="2001-10-01" version="1">MYSPFEILYLAIIIDSLSYTIGALLYGSPIPVRGFKEMGHKMIVNSIYVAVLANIFGLILSILSQLQNIVGANWSTFYLDIGLLQIQTGVAINMGKFLYGIIALILYYFKVPSQFYSLVTPLLQYISFLTDILILLNFYMDLGIFIQSSYMVLIAIGILLMALPFQMGKGIGAMLISFTIVFYVGLPLLPILISNSSPLQNQNFVLQDISLQTEEFYAQIPALFYSFILIPLTYVGVLVGFSVVLQSFIGGYVGRLPIPVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UD1</accession>
    <name>Q97UD1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO3085</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90491">
        <property type="entry name" value="G90491"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989106.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UD1"/>
    <dbReference type="STRING" id="273057.SSO3085"/>
    <dbReference type="TCDB" id="9.B.102.1.10">
        <property type="family name" value="the yede/yeee (yede/yeee) family"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3085"/>
    <dbReference type="EnsemblBacteria" id="AAK43190">
        <property type="protein sequence ID" value="AAK43190"/>
        <property type="gene ID" value="SSO3085"/>
    </dbReference>
    <dbReference type="GeneID" id="84062556"/>
    <dbReference type="KEGG" id="sso:SSO3085"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3192"/>
    <dbReference type="eggNOG" id="arCOG05632">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050656_0_1_2"/>
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    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp_TsuA/YedE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574:SF1">
        <property type="entry name" value="SULPHUR TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
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            <end position="78"/>
        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        <location>
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            <end position="402"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK43190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="409" mass="45378" checksum="990AB67D8F36B84B" modified="2001-10-01" version="1">MPSMVLQPVLQESWALIYGLFALSGFILGWAAQRGNYCFVNAMTSIFTAKSYERFGALLILFGLSALGSGLLVAFGVIPAFDQYYYNYFAGWYILVGSIIFGFGAALAGGCNLSMLYRAASGYVQNWIELFGMMIGTYVFAIGIWPFQLYTMANGILSTTNGGYVEYLPYIIFHSISNMSVYITTLVVSLPLIFIGIYLQQYTKRKWAISSSLRINLPAARAYYNQNISTSGEEKVSLTKEIKDLILLRKPYGTNLTTIILALDLLLVFIVGAGYTFNYLVITSSDGGRFFEYLLMLGNVKLFLNTPWFNDSLPIVDPSTLMVIMLCIGAFSASYLSGDFKIRIPRNKSRLLIGFGGGMLVGIGVRMALGCNVGLMWTNFAQLGYDGIIFLFGMLLGVYLAIKVQERWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UK9</accession>
    <name>Q97UK9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3-hydroxyacyl-CoA dehydrogenase (Hdb-3)</fullName>
            <ecNumber evidence="4">1.1.1.157</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hdb-3</name>
        <name evidence="4" type="ordered locus">SSO2996</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.157" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90480">
        <property type="entry name" value="G90480"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992648.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UK9"/>
    <dbReference type="FunCoup" id="Q97UK9">
        <property type="interactions" value="278"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2996"/>
    <dbReference type="PaxDb" id="273057-SSO2996"/>
    <dbReference type="EnsemblBacteria" id="AAK43102">
        <property type="protein sequence ID" value="AAK43102"/>
        <property type="gene ID" value="SSO2996"/>
    </dbReference>
    <dbReference type="GeneID" id="1453021"/>
    <dbReference type="KEGG" id="sso:SSO2996"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3091"/>
    <dbReference type="eggNOG" id="arCOG00249">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_2_0_2"/>
    <dbReference type="InParanoid" id="Q97UK9"/>
    <dbReference type="PhylomeDB" id="Q97UK9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008691">
        <property type="term" value="F:3-hydroxybutyryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000009">
        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
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        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075:SF5">
        <property type="entry name" value="3-HYDROXYBUTYRYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="3">
        <location>
            <begin position="5"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="2">
        <location>
            <begin position="186"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="2">
        <location>
            <begin position="302"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="384" mass="43166" checksum="0AA068F6F325348B" modified="2001-10-01" version="1">MSIKKIGVVGAGTMGHGIAEVSALANYNVSVVDISWDFLNRAKERIMESLNKFYEKGQIKEKPEDIMKRIEFSTSYDVMRDADFVIEAVPEIIELKRKVFETLDSITPSHTFLASNTSSIPISTIAEVTKRKEKIIGMHFFNPPPIMKLVEIVPSKYTSDETIEVTIDLAKKMNKIPVKLKVEVPGFVSNRIFLRLMQEACREVEDGEATIEEVDSAARNGLKLPMGIFELSDYVGIDVAVDLWNVIVSSGTAQDVKCEMYKRKFEAKELGVKTGKGFYNYPAPGKYRKVELQVTSKVDPARLISLAVNEGAWLIQNGIVNAKDIDTVMIYGFNFPKGLMEIADELGIRNIYNHLVDIYSKGYVAYRPNSLIEELINTSKGFYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UL1</accession>
    <name>Q97UL1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2994</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90480">
        <property type="entry name" value="E90480"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UL1"/>
    <dbReference type="STRING" id="273057.SSO2994"/>
    <dbReference type="PaxDb" id="273057-SSO2994"/>
    <dbReference type="EnsemblBacteria" id="AAK43100">
        <property type="protein sequence ID" value="AAK43100"/>
        <property type="gene ID" value="SSO2994"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2994"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3089"/>
    <dbReference type="eggNOG" id="arCOG08503">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083532_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UL1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="225" mass="26220" checksum="BDE1D6B67A3EDDA5" modified="2001-10-01" version="1">MPNNMSENLYRLVYKTQPFGEIRKVSDRTYSIWGGHYPSFVTFLNLPSEVIKYLPPSRMIEIDPQYYAHIEPLYKSMEKHSEWANDTAYFYGNQVCIALRGCTSFGDEAYSVKDIDQNLNVIKLWLFHIILGSGTIVINGGVKSLEKFLMLRYDKGFEFLESKEEAGKLEIEINDRKIKITLNDDELLSLDYTSSRYIKFSYPLSWKSRYSISDFVSSLKMWKGS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2014-06-11" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UL6</accession>
    <name>PMK_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Phosphomevalonate kinase</fullName>
            <shortName>PMK</shortName>
            <ecNumber>2.7.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2988</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2013" name="J. Biochem." volume="153" first="415" last="420">
            <title>Biochemical evidence supporting the presence of the classical mevalonate pathway in the thermoacidophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Nishimura H."/>
                <person name="Azami Y."/>
                <person name="Miyagawa M."/>
                <person name="Hashimoto C."/>
                <person name="Yoshimura T."/>
                <person name="Hemmi H."/>
            </authorList>
            <dbReference type="PubMed" id="23378249"/>
            <dbReference type="DOI" id="10.1093/jb/mvt006"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>KINETIC PARAMETERS</scope>
        <scope>PATHWAY</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the phosphorylation of (R)-mevalonate 5-phosphate (MVAP) to (R)-mevalonate 5-diphosphate (MVAPP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds such as archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(R)-5-phosphomevalonate + ATP = (R)-5-diphosphomevalonate + ADP</text>
            <dbReference type="Rhea" id="RHEA:16341"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">77 uM for (R,S)-5-phosphomevalonate (at 37 degrees Celsius)</KM>
            <Vmax evidence="2">5.1 umol/min/mg enzyme (at 37 degrees Celsius)</Vmax>
        </kinetics>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GHMP kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90479">
        <property type="entry name" value="F90479"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992639.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UL6"/>
    <dbReference type="SMR" id="Q97UL6"/>
    <dbReference type="FunCoup" id="Q97UL6">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2988"/>
    <dbReference type="PaxDb" id="273057-SSO2988"/>
    <dbReference type="EnsemblBacteria" id="AAK43093">
        <property type="protein sequence ID" value="AAK43093"/>
        <property type="gene ID" value="SSO2988"/>
    </dbReference>
    <dbReference type="GeneID" id="1453012"/>
    <dbReference type="KEGG" id="sso:SSO2988"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3082"/>
    <dbReference type="eggNOG" id="arCOG01032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076270_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UL6"/>
    <dbReference type="PhylomeDB" id="Q97UL6"/>
    <dbReference type="BRENDA" id="2.7.4.2">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q97UL6"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00099"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004631">
        <property type="term" value="F:phosphomevalonate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000186">
        <property type="entry name" value="Phosphomevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053661">
        <property type="entry name" value="GHMP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035102">
        <property type="entry name" value="Phosphomevalonate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040957">
        <property type="entry name" value="Arch_PMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814:SF2">
        <property type="entry name" value="PHOSPHOMEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000429455" description="Phosphomevalonate kinase">
        <location>
            <begin position="1"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="23378249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="323" mass="36208" checksum="D62E48552F5CBDF8" modified="2001-10-01" version="1">MIKVSAPGKILWIGSYSVVFGGISHVIAVNKRVSCSLREIKEKDSLIFHTSYGHFKNSGNELINSVLDTFRERLSQLPQGYEIDLYNDKEFIIDGKKTGLGSSSAATVSLTACLYYAIHGKLDLFEIHKLAQIANYKRQKGIGSGFDIASAVFGSIVYKRFTDLDKMDFYFEKLNLGNYDMMLGFTGKSSETVGLVRKFVEKSNLDDFKEIMRLIDEENYMAIKLIKLNKLDEAVEHIKLGRKYLNYIAERIVGVKLVSKMEEELIKIAEEEGALVALSPGAGGGDSIFALGNDLNRVREAWSKRGIFIIDVKEDEGLRLESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V65</accession>
    <name>Q97V65_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cytosine deaminase (CodA)</fullName>
            <ecNumber evidence="4">3.5.4.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">codA</name>
        <name evidence="4" type="ordered locus">SSO2770</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.4.1" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99453">
        <property type="entry name" value="A99453"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990231.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V65"/>
    <dbReference type="SMR" id="Q97V65"/>
    <dbReference type="FunCoup" id="Q97V65">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2770"/>
    <dbReference type="PaxDb" id="273057-SSO2770"/>
    <dbReference type="EnsemblBacteria" id="AAK42880">
        <property type="protein sequence ID" value="AAK42880"/>
        <property type="gene ID" value="SSO2770"/>
    </dbReference>
    <dbReference type="GeneID" id="1452799"/>
    <dbReference type="KEGG" id="sso:SSO2770"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2852"/>
    <dbReference type="eggNOG" id="arCOG00697">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031758_0_1_2"/>
    <dbReference type="InParanoid" id="Q97V65"/>
    <dbReference type="PhylomeDB" id="Q97V65"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004131">
        <property type="term" value="F:cytosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016814">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01293">
        <property type="entry name" value="Bact_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000019">
        <property type="entry name" value="Cytosine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052349">
        <property type="entry name" value="Metallo-hydrolase_Enzymes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32027">
        <property type="entry name" value="CYTOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32027:SF0">
        <property type="entry name" value="CYTOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase 3" evidence="3">
        <location>
            <begin position="149"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="410" mass="46732" checksum="1C5FFBE98B9EEF1F" modified="2001-10-01" version="1">MFTIRNAKLLDGRIVDVGIEGEKIVCLGECEDNDELIDAENKLVIPPYFNMHFHLDSAFTKAVNKSGTLWEGIRIWQDIRNKISEDEVVRNALTAVKLFVAYGTLWIRSHVDITERSFKLLRAINRVKDESRDIADIQITAFPQDGIFTEKGNDELLRKALENGADNVGLIPHNEITREDGFKSIKYAFELAKEYNKDVDGHIDETDDPNSRYLEVLAKETINNEWYGRVTAGHVTAMHSWDSAYRFRILPYVARAGITVVPNPLINVVLQGRFDGYPKRRGMAPIKEMLYANVNVTLGHDCIMDPWYPLGIGNMLQVLFMAIHLDQMMGINELVSSINLITFNGAKAWRVKNYGIEVGNEANLLVTDADDVIDLIRFMWPPRFVIRKGKVIAKEGKYVLFKGKWERIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V74</accession>
    <name>Q97V74_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbon monoxide dehydrogenase, large chain (CutA-5)</fullName>
            <ecNumber evidence="4">1.2.7.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cutA-5</name>
        <name evidence="4" type="ordered locus">SSO2760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.4" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90451">
        <property type="entry name" value="H90451"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990245.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V74"/>
    <dbReference type="FunCoup" id="Q97V74">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2760"/>
    <dbReference type="PaxDb" id="273057-SSO2760"/>
    <dbReference type="EnsemblBacteria" id="AAK42871">
        <property type="protein sequence ID" value="AAK42871"/>
        <property type="gene ID" value="SSO2760"/>
    </dbReference>
    <dbReference type="GeneID" id="1452790"/>
    <dbReference type="KEGG" id="sso:SSO2760"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2841"/>
    <dbReference type="eggNOG" id="arCOG01167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_2_0_2"/>
    <dbReference type="InParanoid" id="Q97V74"/>
    <dbReference type="PhylomeDB" id="Q97V74"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:anaerobic carbon-monoxide dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.50">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.365.10">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046867">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908:SF132">
        <property type="entry name" value="ALDEHYDE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="MoCoBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20256">
        <property type="entry name" value="MoCoBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde oxidase/xanthine dehydrogenase a/b hammerhead" evidence="3">
        <location>
            <begin position="19"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="762" mass="84892" checksum="5D679AD6AEF9FA42" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V82</accession>
    <name>Q97V82_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2753</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90450">
        <property type="entry name" value="H90450"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990258.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V82"/>
    <dbReference type="STRING" id="273057.SSO2753"/>
    <dbReference type="PaxDb" id="273057-SSO2753"/>
    <dbReference type="EnsemblBacteria" id="AAK42863">
        <property type="protein sequence ID" value="AAK42863"/>
        <property type="gene ID" value="SSO2753"/>
    </dbReference>
    <dbReference type="GeneID" id="1452782"/>
    <dbReference type="KEGG" id="sso:SSO2753"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2832"/>
    <dbReference type="eggNOG" id="arCOG07209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431703_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V82"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="180" mass="21431" checksum="AC97D09124A8CFEF" modified="2001-10-01" version="1">MNRLQQLLKEALDEIEIYGSWVSLYYILKLLAESNVEKLCKEQEVAYHMTVDSLTLFTIYKYGGGIDKTRLFVLSFLLYDYLSRYYNIQNPIFSIKWNKRYFVYSPRIDSRLHTLSKKSLILKKERLYYLNQLGRSEAESINIREKDNAKVDSIVTNLKSLKKVKDIRIFVRRHLLGNDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VA5</accession>
    <name>Q97VA5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallophosphoesterase TT1561-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90448">
        <property type="entry name" value="A90448"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991726.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VA5"/>
    <dbReference type="STRING" id="273057.SSO2729"/>
    <dbReference type="PaxDb" id="273057-SSO2729"/>
    <dbReference type="EnsemblBacteria" id="AAK42840">
        <property type="protein sequence ID" value="AAK42840"/>
        <property type="gene ID" value="SSO2729"/>
    </dbReference>
    <dbReference type="GeneID" id="1452759"/>
    <dbReference type="KEGG" id="sso:SSO2729"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2809"/>
    <dbReference type="eggNOG" id="arCOG01145">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041441_5_0_2"/>
    <dbReference type="InParanoid" id="Q97VA5"/>
    <dbReference type="PhylomeDB" id="Q97VA5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029461">
        <property type="entry name" value="TT1561-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523:SF1">
        <property type="entry name" value="CALCINEURIN-LIKE PHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14582">
        <property type="entry name" value="Metallophos_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophosphoesterase TT1561-like" evidence="1">
        <location>
            <begin position="192"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="302" mass="34851" checksum="283F7BC2D850526A" modified="2001-10-01" version="1">MAKDIKLMFVTDIHGSDIVFRKAINAAHMFKVNYLVFGGDLVGKGVVLLNKKVDEYLTLDNKLITKEEVEEIKRNGYYIYILENRKGMEDFNEVTINRIYVDLVSQQLNSWFKIVRERLEETKVIWNLGNDDPFFVDDIFKNNDIEIKEIIEIDTSSSPLFMLNYAHTNETPYKSYRVAPEYIIYNKGHEMLKKINETKNVIFNFHAPPYNTRLDNAYVNGQWIHVGSRSVRELEEKFQPLLGLHGHIHESSAIDKIGKTLVINPGSMYSEGVLKYAIITISKEVKGISVGYRIKGYVLSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VF7</accession>
    <name>Q97VF7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter, periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2669</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90441">
        <property type="entry name" value="D90441"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923949.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VF7"/>
    <dbReference type="FunCoup" id="Q97VF7">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2669"/>
    <dbReference type="TCDB" id="3.A.1.5.9">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2669"/>
    <dbReference type="EnsemblBacteria" id="AAK42787">
        <property type="protein sequence ID" value="AAK42787"/>
        <property type="gene ID" value="SSO2669"/>
    </dbReference>
    <dbReference type="GeneID" id="1452706"/>
    <dbReference type="KEGG" id="sso:SSO2669"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2751"/>
    <dbReference type="eggNOG" id="arCOG01535">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025114_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VF7"/>
    <dbReference type="PhylomeDB" id="Q97VF7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08509">
        <property type="entry name" value="PBP2_TmCBP_oligosaccharides_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000466">
        <property type="entry name" value="ABC transporter substrate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.105.10:FF:000045">
        <property type="entry name" value="ABC transporter, periplasmic component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290:SF9">
        <property type="entry name" value="OLIGOPEPTIDE-BINDING PROTEIN APPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="5"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="669"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="Solute-binding protein family 5" evidence="5">
        <location>
            <begin position="113"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="693" mass="76687" checksum="E2094D6105BD4E67" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VH3</accession>
    <name>Q97VH3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulatory protein (Lrp/AsnC family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90439">
        <property type="entry name" value="D90439"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988563.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VH3"/>
    <dbReference type="STRING" id="273057.SSO2652"/>
    <dbReference type="PaxDb" id="273057-SSO2652"/>
    <dbReference type="EnsemblBacteria" id="AAK42771">
        <property type="protein sequence ID" value="AAK42771"/>
        <property type="gene ID" value="SSO2652"/>
    </dbReference>
    <dbReference type="GeneID" id="1452690"/>
    <dbReference type="KEGG" id="sso:SSO2652"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2734"/>
    <dbReference type="eggNOG" id="arCOG01585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_4_1_2"/>
    <dbReference type="InParanoid" id="Q97VH3"/>
    <dbReference type="PhylomeDB" id="Q97VH3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013603">
        <property type="entry name" value="TRASH_TR_C_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08394">
        <property type="entry name" value="Arc_trans_TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRASH" evidence="1">
        <location>
            <begin position="118"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="163" mass="18934" checksum="59E3D797BEF46D23" modified="2001-10-01" version="1">MEKLTDLEFRALEILREDSRISVTELSKRLNISRSTATRLLRNLKRRGVKFTIKFQNEPFIAFAISESCQSDECYKLLDGRFINMIRANTLEDLENKLKNVKEKDLVFIGKSSFVVKCDYCGKEIYDNPLTYKVGRKTYYACCNSCLSGLKEKFARNNGLHLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VJ7</accession>
    <name>Q97VJ7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CoA-ligase / coenzyme F390 synthetase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO10784</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90436">
        <property type="entry name" value="D90436"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VJ7"/>
    <dbReference type="STRING" id="273057.SSO10784"/>
    <dbReference type="PaxDb" id="273057-SSO10784"/>
    <dbReference type="EnsemblBacteria" id="AAK42747">
        <property type="protein sequence ID" value="AAK42747"/>
        <property type="gene ID" value="SSO10784"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO10784"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2709"/>
    <dbReference type="eggNOG" id="arCOG02620">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176971_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VJ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43845">
        <property type="entry name" value="BLR5969 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43845:SF1">
        <property type="entry name" value="BLR5969 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="84" mass="10093" checksum="DECB4F2C3EBE9ADA" modified="2001-10-01" version="1">MSVKLTYNEIDPKALTKEEIKEIQRFRLRGLIKRVYESSPYYHKVFKERGLTPDDIRTLEDLVKLPFTTKEDLRRYAYHMVAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-11-27" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VK2</accession>
    <name>Q97VK2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phenylacetic acid degradation paaI protein homolog (PaaI)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">paaI</name>
        <name evidence="3" type="ordered locus">SSO2622</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90435">
        <property type="entry name" value="G90435"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VK2"/>
    <dbReference type="STRING" id="273057.SSO2622"/>
    <dbReference type="PaxDb" id="273057-SSO2622"/>
    <dbReference type="EnsemblBacteria" id="AAK42742">
        <property type="protein sequence ID" value="AAK42742"/>
        <property type="gene ID" value="SSO2622"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2622"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2704"/>
    <dbReference type="eggNOG" id="arCOG00777">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_11_2_2"/>
    <dbReference type="InParanoid" id="Q97VK2"/>
    <dbReference type="PhylomeDB" id="Q97VK2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016289">
        <property type="term" value="F:acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000167">
        <property type="entry name" value="Thioesterase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052723">
        <property type="entry name" value="Acyl-CoA_thioesterase_PaaI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42856">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE PAAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42856:SF1">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE PAAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="2">
        <location>
            <begin position="36"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="121" mass="13691" checksum="DA4200CB4D5DD03A" modified="2001-10-01" version="1">MKESPFLKFLNIELEEIREGYARVSGVVAKDFLNLHNTAHGSFIFAIADAAFEYISNFSRDSVALHMDIDFRRPVKEGEKVIAEAFEESSGKTTSLYRIIVKNEDGKLVAYVTALVYHLDN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-05-10" modified="2025-06-18" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VM6</accession>
    <name>Y2595_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0310 protein SSO2595</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2595</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0310 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK42718" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42718.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90432">
        <property type="entry name" value="G90432"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VM6"/>
    <dbReference type="SMR" id="Q97VM6"/>
    <dbReference type="PaxDb" id="273057-SSO2595"/>
    <dbReference type="EnsemblBacteria" id="AAK42718">
        <property type="protein sequence ID" value="AAK42718"/>
        <property type="gene ID" value="SSO2595"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2595"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2676"/>
    <dbReference type="eggNOG" id="arCOG02727">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754697_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VM6"/>
    <dbReference type="PhylomeDB" id="Q97VM6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd21132">
        <property type="entry name" value="EVE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00771">
        <property type="entry name" value="UPF0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002740">
        <property type="entry name" value="EVE_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022996">
        <property type="entry name" value="UPF0310"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002008">
        <property type="entry name" value="PRK00809.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39661">
        <property type="entry name" value="UPF0310 PROTEIN MJECL36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39661:SF1">
        <property type="entry name" value="UPF0310 PROTEIN MJECL36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01878">
        <property type="entry name" value="EVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000059639" description="UPF0310 protein SSO2595">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="149" mass="17577" checksum="45F7AD44A589FF4D" modified="2004-05-10" version="2">MTYWLVPIQEDMWDVIRDKGIYGYKENLEEFIKEGDYIIIYVSKYYAKRYGGKIVGIVKVLSNWYEDQTPIYPEETVRNKGIYIYRVKVEPVVIGECDMKKILDKIRFIEDKGQIAKYLRNAPANLKRPIPESDAKIVEECLKESLLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VP6</accession>
    <name>Q97VP6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF929 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90430">
        <property type="entry name" value="B90430"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991793.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VP6"/>
    <dbReference type="PaxDb" id="273057-SSO2570"/>
    <dbReference type="EnsemblBacteria" id="AAK42697">
        <property type="protein sequence ID" value="AAK42697"/>
        <property type="gene ID" value="SSO2570"/>
    </dbReference>
    <dbReference type="GeneID" id="1454024"/>
    <dbReference type="KEGG" id="sso:SSO2570"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2652"/>
    <dbReference type="eggNOG" id="arCOG03844">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090239_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VP6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009272">
        <property type="entry name" value="DUF929"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06053">
        <property type="entry name" value="DUF929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="30538" checksum="7D7D43E5BF490DBB" modified="2001-10-01" version="1">MMAKRKKSQQNENKLIYIPFVVLAVVIVFLVAFPYFSHSSSLITANANTPLFNLYKISNTNYASNNSVEIYFVSWYGCPNGATTSWPLYLALSKYGNLSVVPHSSVYESNFGGEIPGLLFLNYTPFPNSRVYFHPIYIYGQYLNETTNGTLINTDDVTFGLKELKSELPSWAYNLVVYYEVNQTYTQLNNVAPAYFGNYPHIITVLIITGPGGTWVDLGYPNSISPSTLATLNSTLLYNIILNKATPSGQYLQAYNEILNASSQITNAINEALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VQ5</accession>
    <name>Q97VQ5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sugar transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2558</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90428">
        <property type="entry name" value="H90428"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923923.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VQ5"/>
    <dbReference type="FunCoup" id="Q97VQ5">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2558"/>
    <dbReference type="PaxDb" id="273057-SSO2558"/>
    <dbReference type="EnsemblBacteria" id="AAK42687">
        <property type="protein sequence ID" value="AAK42687"/>
        <property type="gene ID" value="SSO2558"/>
    </dbReference>
    <dbReference type="GeneID" id="1454014"/>
    <dbReference type="KEGG" id="sso:SSO2558"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2640"/>
    <dbReference type="eggNOG" id="arCOG02691">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_5_2"/>
    <dbReference type="InParanoid" id="Q97VQ5"/>
    <dbReference type="PhylomeDB" id="Q97VQ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045:SF1">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="388" mass="41971" checksum="D504EE1F93A34399" modified="2001-10-01" version="1">MDGYDLGAVVITATILGKLFYPGIGLLSAVLPIVFTVISRPLGGFVFGYVGDKYGRRVSLLISVLGYSLSIGLTSILPTYAQIGILAAVIISILRFIQGIFIGGDVAGSFTIVMESLNNYRGLFSGIMQSGVLVGFVGVDFLFTYLASVTGAQFISTYWRLIFAIGVIPAILAALIRFRMTEPSVWLKIKHSANPLKGLRELPQPFLVMVGFWLAIYAGPQLVPTLFGQVMKLPPSYYGPLVLYMNLIGIPSMLVAGVLSDYIGRRMMGIIGSIFAAIGSLTFYYFIQIHNINLLYLILLFGFLVNLPSSITPAFLSERFKTFTRALGVGTAYNGAYIIAGFAPIYVSILSNFMNPFNAATTVAVIGFIIAITGLIAGPETYKTSLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VY8</accession>
    <name>Q97VY8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Propionyl-CoA carboxylase beta subunit (PpcB)</fullName>
            <ecNumber evidence="3">6.4.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ppcB</name>
        <name evidence="3" type="ordered locus">SSO2463</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.3" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90418">
        <property type="entry name" value="C90418"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923870.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VY8"/>
    <dbReference type="FunCoup" id="Q97VY8">
        <property type="interactions" value="326"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2463"/>
    <dbReference type="PaxDb" id="273057-SSO2463"/>
    <dbReference type="EnsemblBacteria" id="AAK42602">
        <property type="protein sequence ID" value="AAK42602"/>
        <property type="gene ID" value="SSO2463"/>
    </dbReference>
    <dbReference type="GeneID" id="1453929"/>
    <dbReference type="KEGG" id="sso:SSO2463"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2542"/>
    <dbReference type="eggNOG" id="arCOG02705">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018822_6_2_2"/>
    <dbReference type="InParanoid" id="Q97VY8"/>
    <dbReference type="PhylomeDB" id="Q97VY8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009317">
        <property type="term" value="C:acetyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000016">
        <property type="entry name" value="Propionyl-CoA carboxylase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051047">
        <property type="entry name" value="AccD/PCCB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000438">
        <property type="entry name" value="Acetyl_CoA_COase_Trfase_b_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43842">
        <property type="entry name" value="PROPIONYL-COA CARBOXYLASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43842:SF2">
        <property type="entry name" value="PROPIONYL-COA CARBOXYLASE BETA CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01070">
        <property type="entry name" value="ACCCTRFRASEB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="1">
        <location>
            <begin position="8"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="2">
        <location>
            <begin position="270"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="523" mass="57362" checksum="6F1B0A5073F1085A" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-12-07" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VZ6</accession>
    <name>NFI_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease V</fullName>
            <ecNumber evidence="1">3.1.21.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Deoxyinosine 3'endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Deoxyribonuclease V</fullName>
            <shortName evidence="1">DNase V</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nfi</name>
        <name type="ordered locus">SSO2454</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate.</text>
            <dbReference type="EC" id="3.1.21.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the endonuclease V family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.7" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90417">
        <property type="entry name" value="C90417"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923862.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VZ6"/>
    <dbReference type="SMR" id="Q97VZ6"/>
    <dbReference type="FunCoup" id="Q97VZ6">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2454"/>
    <dbReference type="PaxDb" id="273057-SSO2454"/>
    <dbReference type="EnsemblBacteria" id="AAK42594">
        <property type="protein sequence ID" value="AAK42594"/>
        <property type="gene ID" value="SSO2454"/>
    </dbReference>
    <dbReference type="GeneID" id="84060724"/>
    <dbReference type="KEGG" id="sso:SSO2454"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2532"/>
    <dbReference type="eggNOG" id="arCOG00929">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047631_1_1_2"/>
    <dbReference type="InParanoid" id="Q97VZ6"/>
    <dbReference type="PhylomeDB" id="Q97VZ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043737">
        <property type="term" value="F:deoxyribonuclease V activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06559">
        <property type="entry name" value="Endonuclease_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2170.10:FF:000006">
        <property type="entry name" value="Endonuclease V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2170.10">
        <property type="entry name" value="archaeoglobus fulgidus dsm 4304 superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00801">
        <property type="entry name" value="Endonuclease_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007581">
        <property type="entry name" value="Endonuclease-V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511:SF1">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04493">
        <property type="entry name" value="Endonuclease_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000159695" description="Endonuclease V">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with target DNA" evidence="1">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00801"/>
        </source>
    </evidence>
    <sequence length="198" mass="22051" checksum="DE752959A649398B" modified="2001-10-01" version="1">MVEKHLLEFLEKLQFLISKNVKISHYGIENVKKICGVDIAYKGNLGFSVGVSMDINSGDYNYKSYVGEVNFPYIPGFLFMREAPLMIKAIEGLDCHLLLVDGHGIAHPRKSGIAAVIGVLLDFPTIGVAKSRLTGDLVNESEITYVYLNGEKVGVKFGRYFYSPGNKVDLQDCIELGKRGYPKVLKIADMLTKKIKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W26</accession>
    <name>Q97W26_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Inositol monophosphosphatase, putative (Imp3)</fullName>
            <ecNumber evidence="2">3.1.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">imp3</name>
        <name evidence="2" type="ordered locus">SSO2418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90413">
        <property type="entry name" value="E90413"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989410.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W26"/>
    <dbReference type="FunCoup" id="Q97W26">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2418"/>
    <dbReference type="PaxDb" id="273057-SSO2418"/>
    <dbReference type="EnsemblBacteria" id="AAK42564">
        <property type="protein sequence ID" value="AAK42564"/>
        <property type="gene ID" value="SSO2418"/>
    </dbReference>
    <dbReference type="GeneID" id="1453891"/>
    <dbReference type="KEGG" id="sso:SSO2418"/>
    <dbReference type="eggNOG" id="arCOG01349">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_5_0_2"/>
    <dbReference type="InParanoid" id="Q97W26"/>
    <dbReference type="PhylomeDB" id="Q97W26"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01642">
        <property type="entry name" value="Arch_FBPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF4">
        <property type="entry name" value="INOSITOL-1-MONOPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="264" mass="29296" checksum="29B9AA0ECD3FE1F9" modified="2001-10-01" version="1">MINLLNKVAIDATRYLNEIKNEKDLDKVIGFHAGDTTRVIDKKSEEYIFQLLNDTGLKFKFVSEESGVRVPSNSYDYVALIDPLDGSNNFVLGVPWYSISVAIYGKSSNSLLDSLGGFVSHISIDKIYSYDTSSAYLNGNPLKAEIIGNSPSNVVITYFDERGIDKILKVLSALKGYKIRSFGSASLDMILTCMGKAKLYFDIRGKLRNVDIAASANFCKRLNSIPSTLNGKEISSGIDDIYKIDEIIVSRDQSLMKNLYSIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W54</accession>
    <name>Q97W54_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Type II secretion system protein GspF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2386</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90409">
        <property type="entry name" value="G90409"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989455.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W54"/>
    <dbReference type="STRING" id="273057.SSO2386"/>
    <dbReference type="PaxDb" id="273057-SSO2386"/>
    <dbReference type="EnsemblBacteria" id="AAK42534">
        <property type="protein sequence ID" value="AAK42534"/>
        <property type="gene ID" value="SSO2386"/>
    </dbReference>
    <dbReference type="GeneID" id="1453861"/>
    <dbReference type="KEGG" id="sso:SSO2386"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2468"/>
    <dbReference type="eggNOG" id="arCOG01808">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_443878_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W54"/>
    <dbReference type="PhylomeDB" id="Q97W54"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056569">
        <property type="entry name" value="ArlJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402:SF1">
        <property type="entry name" value="TYPE II SECRETION SYSTEM PROTEIN GSPF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="503"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="552"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="583"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="135"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="396"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="616" mass="67930" checksum="111BF488D05509E5" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WH0</accession>
    <name>RAD50_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA double-strand break repair Rad50 ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rad50</name>
        <name type="ordered locus">SSO2249</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per homodimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The two conserved Cys that bind zinc constitute the zinc-hook, which separates the large intramolecular coiled coil regions. The 2 Cys residues coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another Rad50 molecule, thereby forming a V-shaped homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMC family. RAD50 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90395">
        <property type="entry name" value="B90395"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991542.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WH0"/>
    <dbReference type="SMR" id="Q97WH0"/>
    <dbReference type="STRING" id="273057.SSO2249"/>
    <dbReference type="PaxDb" id="273057-SSO2249"/>
    <dbReference type="EnsemblBacteria" id="AAK42417">
        <property type="protein sequence ID" value="AAK42417"/>
        <property type="gene ID" value="SSO2249"/>
    </dbReference>
    <dbReference type="GeneID" id="1453744"/>
    <dbReference type="KEGG" id="sso:SSO2249"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2344"/>
    <dbReference type="eggNOG" id="arCOG00368">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004785_0_2_2"/>
    <dbReference type="InParanoid" id="Q97WH0"/>
    <dbReference type="PhylomeDB" id="Q97WH0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990391">
        <property type="term" value="C:DNA repair complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004529">
        <property type="term" value="F:DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.510">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00449">
        <property type="entry name" value="RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022982">
        <property type="entry name" value="Rad50_ATPase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013134">
        <property type="entry name" value="Zn_hook_RAD50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114:SF2">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04423">
        <property type="entry name" value="Rad50_zn_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75712">
        <property type="entry name" value="Rad50 coiled-coil Zn hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51131">
        <property type="entry name" value="ZN_HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000138665" description="DNA double-strand break repair Rad50 ATPase">
        <location>
            <begin position="1"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc-hook" evidence="1">
        <location>
            <begin position="380"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="176"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="376"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="440"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="32"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00449"/>
        </source>
    </evidence>
    <sequence length="864" mass="101602" checksum="657076AEA9B709FC" modified="2001-09-26" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-08-29" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WM6</accession>
    <name>PHS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Putative pterin-4-alpha-carbinolamine dehydratase</fullName>
            <shortName>PHS</shortName>
            <ecNumber>4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Pterin carbinolamine dehydratase</fullName>
            <shortName>PCD</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2187</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK42360" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="4.2.1.96"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42360.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99388">
        <property type="entry name" value="A99388"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992110.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WM6"/>
    <dbReference type="SMR" id="Q97WM6"/>
    <dbReference type="FunCoup" id="Q97WM6">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2187"/>
    <dbReference type="PaxDb" id="273057-SSO2187"/>
    <dbReference type="EnsemblBacteria" id="AAK42360">
        <property type="protein sequence ID" value="AAK42360"/>
        <property type="gene ID" value="SSO2187"/>
    </dbReference>
    <dbReference type="GeneID" id="1453687"/>
    <dbReference type="KEGG" id="sso:SSO2187"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2283"/>
    <dbReference type="eggNOG" id="arCOG02939">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081974_4_5_2"/>
    <dbReference type="InParanoid" id="Q97WM6"/>
    <dbReference type="PhylomeDB" id="Q97WM6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00488">
        <property type="entry name" value="PCD_DCoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00434">
        <property type="entry name" value="Pterin_4_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002017">
        <property type="entry name" value="PRK00823.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF0">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="PCD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000063111" description="Putative pterin-4-alpha-carbinolamine dehydratase">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="97" mass="11191" checksum="B0C87D24BDFF7B42" modified="2004-05-24" version="2">MSGISSKELEELKINGWIVLENGKKIKKEFRFKDFKQSVDFLKDIQPSADALDHHPDVCVYYNRVVVELTTHDVGGLTDLDYKLAIKLDELYKMKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WU8</accession>
    <name>Q97WU8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protease, putative</fullName>
            <ecNumber evidence="1">3.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90368">
        <property type="entry name" value="C90368"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923724.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WU8"/>
    <dbReference type="STRING" id="273057.SSO2015"/>
    <dbReference type="PaxDb" id="273057-SSO2015"/>
    <dbReference type="EnsemblBacteria" id="AAK42202">
        <property type="protein sequence ID" value="AAK42202"/>
        <property type="gene ID" value="SSO2015"/>
    </dbReference>
    <dbReference type="GeneID" id="1453529"/>
    <dbReference type="KEGG" id="sso:SSO2015"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2092"/>
    <dbReference type="eggNOG" id="arCOG03202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053295_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WU8"/>
    <dbReference type="PhylomeDB" id="Q97WU8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001539">
        <property type="entry name" value="Peptidase_U32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051454">
        <property type="entry name" value="RNA/ubiquinone_mod_enzymes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30217:SF10">
        <property type="entry name" value="23S RRNA 5-HYDROXYCYTIDINE C2501 SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30217">
        <property type="entry name" value="PEPTIDASE U32 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01136">
        <property type="entry name" value="Peptidase_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="385" mass="44158" checksum="C777825CDE0508C3" modified="2001-10-01" version="1">MKLVVATNFDDSLLEELKKYPEVKYIFGSFKRTITGHGRAGFIIPDIKEEQFKNHISIAHSYGIKFLYTMNTNTLLGKEYDAEFIGKVMKEIDKLVSLGVDGFIIAVPFLIRLIRSEYPDLEVSASSFSRIHNVREVEEYANLGVNTIIMHEDANRDFKLLKEIASLSKTNKFDVELILNNSCLYGCPFRLTHDGISSITSMVNGVNDVWFEYPILLCATDVLNDPVNLIRSRWIRPEDIKYYEEIGINRFKIAGRNKKTEWILNVVKAFTERMYEGDLLDLVSYPQGRAATKAIQMVNGPSSYSVLTKVKIDNTKFPEDGIKFFLTNDCDTRSCKECKYCDIIAERVITIDGEPFKTDKWNIRQSYPINLIPKFKENGIKKGNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WV1</accession>
    <name>Q97WV1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PaREP1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90367">
        <property type="entry name" value="H90367"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923722.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WV1"/>
    <dbReference type="STRING" id="273057.SSO2013"/>
    <dbReference type="PaxDb" id="273057-SSO2013"/>
    <dbReference type="DNASU" id="1453526"/>
    <dbReference type="EnsemblBacteria" id="AAK42199">
        <property type="protein sequence ID" value="AAK42199"/>
        <property type="gene ID" value="SSO2013"/>
    </dbReference>
    <dbReference type="GeneID" id="1453526"/>
    <dbReference type="KEGG" id="sso:SSO2013"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2086"/>
    <dbReference type="eggNOG" id="arCOG03721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115256_2_0_2"/>
    <dbReference type="InParanoid" id="Q97WV1"/>
    <dbReference type="PhylomeDB" id="Q97WV1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="14304" checksum="8321281D52719726" modified="2001-10-01" version="1">MRRPSKDLALKFLNEAEEFLSKGDLVQACEKYYKAAEEAIKLLVIENNLKEIINDVESKGRWESENLFKASKLLRSNNLEILILWKSAWTLHVEGFHELSLNEKEVKKLKEDVKKLVIFAINS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WW0</accession>
    <name>CS3HD_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated endonuclease Cas3-HD</fullName>
            <ecNumber>3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cas3</name>
        <name type="synonym">cas3''</name>
        <name type="ordered locus">SSO2001</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2009" name="FEBS Lett." volume="583" first="771" last="776">
            <title>Characterization of the endonuclease SSO2001 from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Han D."/>
                <person name="Krauss G."/>
            </authorList>
            <dbReference type="PubMed" id="19174159"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2009.01.024"/>
        </citation>
        <scope>FUNCTION AS AN ENDONUCLEASE</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>COFACTOR</scope>
        <scope>MUTAGENESIS OF ASP-63; GLU-92 AND SER-95</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity. Acts as a dsDNA and dsRNA endonuclease (in a fusion with Alicyclus caldarius esterase to increase solubility).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="4">Optimum pH is 3 and 7-8. Expressed as a fusion protein to increase stability.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="4">Optimum temperature is 50 degrees Celsius. Expressed as a fusion protein to increase stability.</text>
        </temperatureDependence>
    </comment>
    <comment type="domain">
        <text>Proteins of this family have an N-terminal nuclease domain and a C-terminal helicase/ATPase domain. In some CRISPR/Cas systems the domains are swapped, in others they are encoded separately.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CRISPR-associated nuclease Cas3-HD family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90366">
        <property type="entry name" value="G90366"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923717.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WW0"/>
    <dbReference type="SMR" id="Q97WW0"/>
    <dbReference type="STRING" id="273057.SSO2001"/>
    <dbReference type="PaxDb" id="273057-SSO2001"/>
    <dbReference type="DNASU" id="1453517"/>
    <dbReference type="EnsemblBacteria" id="AAK42190">
        <property type="protein sequence ID" value="AAK42190"/>
        <property type="gene ID" value="SSO2001"/>
    </dbReference>
    <dbReference type="GeneID" id="1453517"/>
    <dbReference type="KEGG" id="sso:SSO2001"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2077"/>
    <dbReference type="eggNOG" id="arCOG01442">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1163752_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WW0"/>
    <dbReference type="BRENDA" id="3.1.99.B5">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10013">
        <property type="entry name" value="Cas3''_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006483">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038257">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01596">
        <property type="entry name" value="cas3_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51643">
        <property type="entry name" value="HD_CAS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000417608" description="CRISPR-associated endonuclease Cas3-HD">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="HD Cas3-type" evidence="3">
        <location>
            <begin position="5"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Significant loss of endonuclease activity. Complete loss of activity; when associated with A-92." evidence="4">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Some loss of endonuclease activity. Complete loss of activity; when associated with A-63." evidence="4">
        <original>E</original>
        <variation>A</variation>
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect." evidence="4">
        <original>S</original>
        <variation>A</variation>
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="19174159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="239" mass="27515" checksum="1BE0167CDE783D42" modified="2001-10-01" version="1">MIKPCAYEKQGLIDHAIGSYRVLDGKISESYYKIISRRLERYGIVLDLNGVKEMVKDVVVLHDMGKAGEYYQNQFDDNCNPLKSNFSFIYHELGSALFFYYDYEPIDVEKAEEVKSLLTLAVLNHLNAIRVISDYLVNKFPDNFDERMIKLNKYGSIMLQNLRGVISKSLKVRDYTFDDYHDMLYAFSKKSDKYLKLYNLFLAPIMLGDNLDSSLVRNNGSKTGFVRILEGELNGGSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WX6</accession>
    <name>Q97WX6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1985</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90364">
        <property type="entry name" value="H90364"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993004.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WX6"/>
    <dbReference type="PaxDb" id="273057-SSO1985"/>
    <dbReference type="EnsemblBacteria" id="AAK42175">
        <property type="protein sequence ID" value="AAK42175"/>
        <property type="gene ID" value="SSO1985"/>
    </dbReference>
    <dbReference type="GeneID" id="1453502"/>
    <dbReference type="KEGG" id="sso:SSO1985"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2060"/>
    <dbReference type="eggNOG" id="arCOG10265">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099323_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WX6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="193" mass="22168" checksum="BE4F5A78B81DED5A" modified="2001-10-01" version="1">MPPDQQKNKNYYQYGVFWLYNWFQAENNNMNIFFLINGNIYNYSLALEQYIWDSNCWLLECSPFSAYAIPPSPPASTNTGEPIFAQSYINAYNTAFNQETSGYNLNYQKALNNYNKFTYSDVNSNFVPGTFGYKEEYNTPSQRFYINYLQDDAGVYVGTPYLFVSAGSGGGSGFMYLIWIIVTYGVPYVVNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X22</accession>
    <name>Q97X22_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gdhA-3</name>
        <name evidence="10" type="ordered locus">SSO1930</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90358">
        <property type="entry name" value="G90358"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X22"/>
    <dbReference type="FunCoup" id="Q97X22">
        <property type="interactions" value="251"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1930"/>
    <dbReference type="PaxDb" id="273057-SSO1930"/>
    <dbReference type="EnsemblBacteria" id="AAK42126">
        <property type="protein sequence ID" value="AAK42126"/>
        <property type="gene ID" value="SSO1930"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1930"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2005"/>
    <dbReference type="eggNOG" id="arCOG01352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_1_2_2"/>
    <dbReference type="InParanoid" id="Q97X22"/>
    <dbReference type="PhylomeDB" id="Q97X22"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="201"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="7">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="434" mass="47351" checksum="B2C323D636039419" modified="2001-10-01" version="1">MSWNNFLFLKYLIKAMEELLTSNLFVQQVKKLYKVGELLGLDIDTLEALSQPERVIQVKIQIRGSDGKLRTFVGWRSQHNSALGPYKGGVRYSPNVTQEEVIALSMIMTWKNSLLLLPYGGGKGGIRVDPKKLTLKELEDLSRKYIQLIHNYLGSNVDIPAPDINTNPQTMAWFLDEYIKITGEVDFAVFTGKPYELGGIGVRLYSTGLGVATIAREAANKFIGGIEGSRVIIQGFGNVGSFTAKFLSEMGAKIIGVSDIGGGVINENGIDVNKALEVVQRTGSVVNYPEGKKVTNEELLISDCDILIPAAVENVINKFNAPKVKAKLIVEGANGPLTADADDVIKQSGIVVIPDILANAGGVVGSYVEWANNKSGGIISDEEAKKLIIDRMTNAFNTLYDFHKRKFADQDLRTVAMALAVDRVVKAMKARGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X68</accession>
    <name>Q97X68_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO9136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90352">
        <property type="entry name" value="C90352"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923638.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X68"/>
    <dbReference type="PaxDb" id="273057-SSO9136"/>
    <dbReference type="EnsemblBacteria" id="AAK42074">
        <property type="protein sequence ID" value="AAK42074"/>
        <property type="gene ID" value="SSO9136"/>
    </dbReference>
    <dbReference type="GeneID" id="1453401"/>
    <dbReference type="KEGG" id="sso:SSO9136"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1939"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2476064_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X68"/>
    <dbReference type="PhylomeDB" id="Q97X68"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="10"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="91" mass="10731" checksum="A5F7DC99F225E9AC" modified="2001-10-01" version="1">MSGNRVRLFKRRALRFLDEAKRDLNEGYYDIGSFHVEQALQLYIKAVIFELFGKEYEGHGIRELLGYLSKLLKENEYEELAKKVNERVSGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XA6</accession>
    <name>Q97XA6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Coenzyme PQQ synthesis protein E (PqqE-5)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pqqE-5</name>
        <name evidence="8" type="ordered locus">SSO1840</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90347">
        <property type="entry name" value="E90347"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992721.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XA6"/>
    <dbReference type="FunCoup" id="Q97XA6">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1840"/>
    <dbReference type="PaxDb" id="273057-SSO1840"/>
    <dbReference type="DNASU" id="1453363"/>
    <dbReference type="EnsemblBacteria" id="AAK42036">
        <property type="protein sequence ID" value="AAK42036"/>
        <property type="gene ID" value="SSO1840"/>
    </dbReference>
    <dbReference type="GeneID" id="1453363"/>
    <dbReference type="KEGG" id="sso:SSO1840"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1900"/>
    <dbReference type="eggNOG" id="arCOG00938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_0_2"/>
    <dbReference type="InParanoid" id="Q97XA6"/>
    <dbReference type="PhylomeDB" id="Q97XA6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21123">
        <property type="entry name" value="SPASM_MftC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017200">
        <property type="entry name" value="PqqE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04053">
        <property type="entry name" value="TIGR04053 family radical SAM/SPASM domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF34">
        <property type="entry name" value="TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE COFACTOR MODIFYING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037420">
        <property type="entry name" value="PQQ_syn_pqqE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="4"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="350" mass="39515" checksum="AB5F66BA1360721E" modified="2001-10-01" version="1">MPFENAPHLVFWEVTKACPLSCKHCRANAIDKPLPGELSTEESKKLLEDIARFGKVVIVFTGGDPLSRSDIFEIMEYAKSLGLIVSIAPSPSYRLRDETMKMISNYARYMSISIDGATSQTHDWLRGLGSYNYALRGIELGLKYGIQVQVNTLVWKKSYSELPFLVKLLKELGVKVWEVFFLIPVGRGVIELDIPKEKYKDVIDFLVETTRYDLVVRTVEAPFFRRAKLEYTPSTIKSNELVSKLRELLGEPVKDVDKSVLPTRDGSGVIFIGYNGDVYPSGFLPLYLGNVRKESIVDIYRKSEVLKIIKDGRFDGKCGVCKFNNICGGSRARAFAVYNNPFAEDPMCPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XB0</accession>
    <name>Q97XB0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90346">
        <property type="entry name" value="H90346"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992714.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XB0"/>
    <dbReference type="STRING" id="273057.SSO1835"/>
    <dbReference type="PaxDb" id="273057-SSO1835"/>
    <dbReference type="EnsemblBacteria" id="AAK42031">
        <property type="protein sequence ID" value="AAK42031"/>
        <property type="gene ID" value="SSO1835"/>
    </dbReference>
    <dbReference type="GeneID" id="1453358"/>
    <dbReference type="KEGG" id="sso:SSO1835"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1895"/>
    <dbReference type="eggNOG" id="arCOG01204">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130257_9_0_2"/>
    <dbReference type="InParanoid" id="Q97XB0"/>
    <dbReference type="PhylomeDB" id="Q97XB0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052548">
        <property type="entry name" value="Type_VII_TA_antitoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933:SF1">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE MNTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="126" mass="14761" checksum="5CEF9624BC49C3B8" modified="2001-10-01" version="1">MLERLCEIYSKFTPKLVVIFGSYARGDYTDQSDIDVLIVSDVFPRDPRKGFAITYSIEFPKVMPIAMNTQVFLKKLEGGSTFILEIIEDGKILCGDEEFKKEVLKKYSEIRRKFKRKGKLWEYMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XF5</accession>
    <name>Q97XF5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90340">
        <property type="entry name" value="D90340"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XF5"/>
    <dbReference type="PaxDb" id="273057-SSO8910"/>
    <dbReference type="EnsemblBacteria" id="AAK41979">
        <property type="protein sequence ID" value="AAK41979"/>
        <property type="gene ID" value="SSO8910"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8910"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1838"/>
    <dbReference type="eggNOG" id="arCOG08402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2243911_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XF5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="91" mass="10082" checksum="B3F9B3DF27DCB592" modified="2001-10-01" version="1">MMNSILMTGYAHESRGIKVVKVTARYLDAGDVLLERDSIAPLNLATNTDERVVVFPSTSPNELRVSVYEPSRGVPVTELEVVKSKQKLRHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XH9</accession>
    <name>Q97XH9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1762</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90337">
        <property type="entry name" value="D90337"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989071.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XH9"/>
    <dbReference type="STRING" id="273057.SSO1762"/>
    <dbReference type="PaxDb" id="273057-SSO1762"/>
    <dbReference type="EnsemblBacteria" id="AAK41955">
        <property type="protein sequence ID" value="AAK41955"/>
        <property type="gene ID" value="SSO1762"/>
    </dbReference>
    <dbReference type="GeneID" id="1454734"/>
    <dbReference type="KEGG" id="sso:SSO1762"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1809"/>
    <dbReference type="eggNOG" id="arCOG06025">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154392_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XH9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="240" mass="27670" checksum="A4CB2B2C64864FBE" modified="2001-10-01" version="1">MNRGKVRNHALYFLGVLTYVVALIPFLTVNLVRTLILAPIIIYTLPIMEYLQPKVMSLKIGYKDILLMIPPIIPYVFLPYNEQSIYILIPLALMLLTFTLYLAKYTMWGNVIGTAFEASISIVWGLFVHNFLFLIPSIYWLLYIFVGALYVEYKIPFRRLNKRIVQISWIISLVSLIVLSLKNPITLITLLEPSTRYLIPGEKLKSTKEIKDLGKRGSKRDMLFVALLAITYTFSIVFPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XJ1</accession>
    <name>Q97XJ1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1058</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90335">
        <property type="entry name" value="F90335"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XJ1"/>
    <dbReference type="PaxDb" id="273057-SSO1745"/>
    <dbReference type="EnsemblBacteria" id="AAK41941">
        <property type="protein sequence ID" value="AAK41941"/>
        <property type="gene ID" value="SSO1745"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1745"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1789"/>
    <dbReference type="eggNOG" id="arCOG02754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102439_1_0_2"/>
    <dbReference type="InParanoid" id="Q97XJ1"/>
    <dbReference type="PhylomeDB" id="Q97XJ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053172">
        <property type="entry name" value="Tn903_transposase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631:SF3">
        <property type="entry name" value="ISSOD12 TRANSPOSASE TNPA_ISSOD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="105" mass="12896" checksum="C5F89B4C649ED7F9" modified="2001-10-01" version="1">MGKSKYKRDWSKYDENVITRYKLMFPFYVFEHWWDLLAEENRNARTKYKAPKEFNDFLAFLHIFLPCNRRSIKSLRTIENHPHKPRLLNNMGESEEHEHNLPRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XK4</accession>
    <name>Q97XK4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1729</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99334">
        <property type="entry name" value="A99334"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XK4"/>
    <dbReference type="STRING" id="273057.SSO1729"/>
    <dbReference type="PaxDb" id="273057-SSO1729"/>
    <dbReference type="EnsemblBacteria" id="AAK41928">
        <property type="protein sequence ID" value="AAK41928"/>
        <property type="gene ID" value="SSO1729"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1729"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1772"/>
    <dbReference type="eggNOG" id="arCOG02666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012640_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XK4"/>
    <dbReference type="PhylomeDB" id="Q97XK4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09679">
        <property type="entry name" value="Cas10_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2220">
        <property type="entry name" value="CRISPR-Cas system, Cmr2 subunit, D1 domain, cysteine cluster"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054767">
        <property type="entry name" value="Cas10-Cmr2_palm2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038242">
        <property type="entry name" value="Cmr2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024615">
        <property type="entry name" value="CRISPR-assoc_Cmr2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013407">
        <property type="entry name" value="CRISPR-assoc_prot_Cmr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02577">
        <property type="entry name" value="cas_TM1794_Cmr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22335">
        <property type="entry name" value="Cas10-Cmr2_palm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12469">
        <property type="entry name" value="Cmr2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="CRISPR-associated protein Cmr2 N-terminal" evidence="4">
        <location>
            <begin position="233"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Cas10/Cmr2 second palm" evidence="5">
        <location>
            <begin position="682"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="991" mass="116431" checksum="3E3DD08D4CC70D80" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XK5</accession>
    <name>Q97XK5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1728</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90333">
        <property type="entry name" value="H90333"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923685.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XK5"/>
    <dbReference type="STRING" id="273057.SSO1728"/>
    <dbReference type="PaxDb" id="273057-SSO1728"/>
    <dbReference type="EnsemblBacteria" id="AAK41927">
        <property type="protein sequence ID" value="AAK41927"/>
        <property type="gene ID" value="SSO1728"/>
    </dbReference>
    <dbReference type="GeneID" id="1454706"/>
    <dbReference type="KEGG" id="sso:SSO1728"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1771"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077283_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XK5"/>
    <dbReference type="PhylomeDB" id="Q97XK5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="354" mass="40279" checksum="305C2026BD83B2CC" modified="2001-10-01" version="1">MTKELTREEYYKALEKAVNEVILSMTGTRKDVAKRLLLGAVVGRNATEIAQEAEMNYETVLNNLDKAAQVNLIEVVKKLVGDHPVLLIIDDTHDHKLYARAMPVSRNGAQIFYCRAHKRFESAIQLLVIGVKDLVNNQIYVIHIIAYIPRKVEEELKRRGEEVKFKTKIDALLEFLSSLSGLNVKSKVFDSWYVNSRTLQGNTVGELKSSARVVESGRSVPVSEFPQGEYLVEYLGTPIKLLVIDDYKGYGRRYFFSTDLNDTAEDIITTWENRWDIEVLIRELKALGLEGGSFLTWVRNSGFVALKALSLLVVQYFKYSTGLVLGAKRLARLIKSIYREAGGIKKLFKRRRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XR7</accession>
    <name>Q97XR7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1641</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90324">
        <property type="entry name" value="H90324"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923542.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XR7"/>
    <dbReference type="STRING" id="273057.SSO1641"/>
    <dbReference type="PaxDb" id="273057-SSO1641"/>
    <dbReference type="EnsemblBacteria" id="AAK41855">
        <property type="protein sequence ID" value="AAK41855"/>
        <property type="gene ID" value="SSO1641"/>
    </dbReference>
    <dbReference type="GeneID" id="7806661"/>
    <dbReference type="KEGG" id="sso:SSO1641"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1688"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123170_2_2_2"/>
    <dbReference type="InParanoid" id="Q97XR7"/>
    <dbReference type="PhylomeDB" id="Q97XR7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="9"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="129" mass="14814" checksum="DD64EF7919C8E5D5" modified="2001-10-01" version="1">MREEAEKWLRQALEDLATAKDTITTGHYYASAFWAEQAAEKALKALLIAGGKIERTHDLNELLEIIKEEIGLSVEEIRSEVIKLTLHYTISRYPDAANTIPYSLYSKEDAEELVKKAEKVIEWVKRNLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XT4</accession>
    <name>Q97XT4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase FkbM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90322">
        <property type="entry name" value="G90322"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XT4"/>
    <dbReference type="STRING" id="273057.SSO1623"/>
    <dbReference type="PaxDb" id="273057-SSO1623"/>
    <dbReference type="EnsemblBacteria" id="AAK41838">
        <property type="protein sequence ID" value="AAK41838"/>
        <property type="gene ID" value="SSO1623"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1623"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1666"/>
    <dbReference type="eggNOG" id="arCOG01400">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063181_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XT4"/>
    <dbReference type="PhylomeDB" id="Q97XT4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052514">
        <property type="entry name" value="SAM-dependent_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203">
        <property type="entry name" value="METHYLTRANSFERASE, FKBM FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203:SF15">
        <property type="entry name" value="SLL1173 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase FkbM" evidence="1">
        <location>
            <begin position="200"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="372" mass="43246" checksum="1831BCC11C91A080" modified="2001-10-01" version="1">MSYFIFRKLDINNSYILFLLFLILLSLLVLRVTAIRLYLNLSHEEVYLMKVINKSRETLYNIYYTLYKINVYRKIFSNWFYVIHKLRKGEKDIDVVLKNGSKGKCNIKCILTLTTIISEFSSINSRKFNFDDNNRLYYDNNLIIQENNTTWLLSIGGFIKNNDYWFFPKYNVKFNTIGYGIFETFIIEQYYTEIQGEVIDIGANIGDSAIYFALKGASHVYAFEPLPLVYKAALQNIKLNNLENKITLINGAVGSKEGKVKVPLNINLEDSGGFYITNQGDVEVPLFSFDDIVKNMVKDPYLLKMDCEGCEADIILNSDSISYFEKILVESHPHITKVSDKALLSKLRKLKYKCEERYRVGKTKLFYCSKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XW3</accession>
    <name>Q97XW3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Partial transposase ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO1598</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90318">
        <property type="entry name" value="G90318"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009071332.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XW3"/>
    <dbReference type="FunCoup" id="Q97XW3">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1598"/>
    <dbReference type="PaxDb" id="273057-SSO1598"/>
    <dbReference type="EnsemblBacteria" id="AAK41806">
        <property type="protein sequence ID" value="AAK41806"/>
        <property type="gene ID" value="SSO1598"/>
    </dbReference>
    <dbReference type="GeneID" id="84061006"/>
    <dbReference type="KEGG" id="sso:SSO1598"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1631"/>
    <dbReference type="eggNOG" id="arCOG04100">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016513_2_1_2"/>
    <dbReference type="InParanoid" id="Q97XW3"/>
    <dbReference type="PhylomeDB" id="Q97XW3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01154">
        <property type="entry name" value="AidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052904">
        <property type="entry name" value="Acyl-CoA_dehydrogenase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034184">
        <property type="entry name" value="AidB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041504">
        <property type="entry name" value="AidB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42707:SF2">
        <property type="entry name" value="ACD11 DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42707">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18158">
        <property type="entry name" value="AidB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adaptive response protein AidB N-terminal" evidence="9">
        <location>
            <begin position="16"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="162"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="274"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="435"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK41806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="546" mass="62399" checksum="75048B1764E9A537" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XX5</accession>
    <name>Q97XX5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thermopsin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="C90317"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923484.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XX5"/>
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    <dbReference type="EnsemblBacteria" id="AAK41794">
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        <property type="gene ID" value="SSO1584"/>
    </dbReference>
    <dbReference type="GeneID" id="7806186"/>
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    <dbReference type="eggNOG" id="arCOG06001">
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    </dbReference>
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    <dbReference type="PhylomeDB" id="Q97XX5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="55041" checksum="EA54AD8C0B43A41D" modified="2001-10-01" version="1">MRPKMLLLIPILLSLPTLALAANSSSPSLYVMYQQYSSTLQITPSHITYKYSSSFYNTTTSSTNDERTIKYNSNSFNVNVGPLTNYTGKVSVNVSPFSISITSQIPQLARVLLIEPGLIEEVVWAGFTNTTTTVKGLTYFNNTATLVLQFLNGSNFANITFTISHTQTTYQKALTLLLHKVNLTMTGEYQLTLTIQGTQPQRYSKMEFNYEGIRAQAPYNTSIAYYNGTYVPAMIWSSKASGLLATELTNLQGIVEFTDIEFFGVNGSVVGSLDNTFSSIYFTHQGFILNITYNSLNSRIKIVINPNAEYAKASVATTVLVNGNPAVVVITDQGVESTATVNLYHKVMVFGPTTLIYVNTTSSGAYITIFPNGSYEYVSQVEPSVTVSNITLGGKTYTTQIVNINNSSNYVIFNVSMAKNETFAVFKQGSNGMVELNPNNYFIYNGKIVVFDDPSMTYYIVYGYQPSSQSFSISSNLLLPIIIAIVFIVVVAILALRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y27</accession>
    <name>Q97Y27_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cas12f1-like TNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO8288</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99311">
        <property type="entry name" value="A99311"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y27"/>
    <dbReference type="SMR" id="Q97Y27"/>
    <dbReference type="STRING" id="273057.SSO8288"/>
    <dbReference type="PaxDb" id="273057-SSO8288"/>
    <dbReference type="DNASU" id="1454523"/>
    <dbReference type="EnsemblBacteria" id="AAK41744">
        <property type="protein sequence ID" value="AAK41744"/>
        <property type="gene ID" value="SSO8288"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8288"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134213_1_0_2"/>
    <dbReference type="InParanoid" id="Q97Y27"/>
    <dbReference type="PhylomeDB" id="Q97Y27"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="17"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="93" mass="10948" checksum="258508A1CB70ABA2" modified="2001-10-01" version="1">MKDLPSEFRDKLYLMQYRRVRYWVEWQAKKHDLLVQYVNPGYSSVSCPKCGKRMVEVSHRWFKCGCGYENDRDVIAITNLNGRGSLILSTALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y39</accession>
    <name>Q97Y39_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR type III-associated protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1510</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90309">
        <property type="entry name" value="E90309"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y39"/>
    <dbReference type="STRING" id="273057.SSO1510"/>
    <dbReference type="PaxDb" id="273057-SSO1510"/>
    <dbReference type="EnsemblBacteria" id="AAK41732">
        <property type="protein sequence ID" value="AAK41732"/>
        <property type="gene ID" value="SSO1510"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1510"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1550"/>
    <dbReference type="eggNOG" id="arCOG02661">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053305_3_0_2"/>
    <dbReference type="InParanoid" id="Q97Y39"/>
    <dbReference type="PhylomeDB" id="Q97Y39"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09661">
        <property type="entry name" value="Cmr6_III-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010172">
        <property type="entry name" value="CRISPR-assoc_prot_TM1791"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01898">
        <property type="entry name" value="cas_TM1791_cmr6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39965">
        <property type="entry name" value="CRISPR SYSTEM CMR SUBUNIT CMR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39965:SF1">
        <property type="entry name" value="CRISPR SYSTEM CMR SUBUNIT CMR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="2">
        <location>
            <begin position="91"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="246" mass="28288" checksum="E2BE66FE7AD16C26" modified="2001-10-01" version="1">MSIIMERQRGNQLLRIISEYMTNLKEKGQKLAKDNTMENLVNFTPRYNLIKSYLDDVERALDRSGLCYVKITFITLSKLLTGWSPIYFITEVPLAWDMILDTPYIAGSEIKGIVKNYFKEVTSNDKVESCLYGDEGKMGKVIFFNAYPIDGKNVLTYDIITPHYNGAKDEYNVKPIPIKFLAINKGITFKTYLAFDNKELNECGKDSLYLLLKTMIFSMRIGWGRKVTRGYGSLDIKEMDVKCHGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y62</accession>
    <name>Q97Y62_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First part of one of two inversely orientated ORFs in ISC1043</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO8193</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90306">
        <property type="entry name" value="F90306"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y62"/>
    <dbReference type="STRING" id="273057.SSO8193"/>
    <dbReference type="PaxDb" id="273057-SSO8193"/>
    <dbReference type="EnsemblBacteria" id="AAK41709">
        <property type="protein sequence ID" value="AAK41709"/>
        <property type="gene ID" value="SSO8193"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8193"/>
    <dbReference type="HOGENOM" id="CLU_2550446_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y62"/>
    <dbReference type="PhylomeDB" id="Q97Y62"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="82" mass="9396" checksum="8A9D51E1B2CA6F45" modified="2001-10-01" version="1">MDPLFSDLIYDPPCPPFNEDQSLSRLKYSSKYSSGFFMNFNIISLHLFPPLIVALGYQVDLRIKEIIQCRLAIVLISPQEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y80</accession>
    <name>Q97Y80_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1458</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90303">
        <property type="entry name" value="F90303"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989137.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y80"/>
    <dbReference type="STRING" id="273057.SSO1458"/>
    <dbReference type="PaxDb" id="273057-SSO1458"/>
    <dbReference type="EnsemblBacteria" id="AAK41685">
        <property type="protein sequence ID" value="AAK41685"/>
        <property type="gene ID" value="SSO1458"/>
    </dbReference>
    <dbReference type="GeneID" id="1454464"/>
    <dbReference type="KEGG" id="sso:SSO1458"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1488"/>
    <dbReference type="eggNOG" id="arCOG04294">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122250_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y80"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="191" mass="22207" checksum="EB415FF67F50AF58" modified="2001-10-01" version="1">MLTTGFPFLDEIIKRDEIVEFFSTDNDLLRTFYHRVIAISAPVYVVVVSERGGLDPYLIERFQNIFDNHAEVYIRRAFKAEDVPITIDSMGDNDLIIIDPYHYRKNYTKIVSSLRKVKGRKFLFSFMDREREGSIFGSHTSHSMIKLIKSTRGFKFIIVKSVTSREIEIPNYLLDLYGKVSDDGGLLKYMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YA1</accession>
    <name>Q97YA1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR type III-associated protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1431</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2023" name="Curr. Res. Struct. Biol." volume="5" first="100098" last="100098">
            <title>Structure of the &lt;i&gt;Saccharolobus solfataricus&lt;/i&gt; type III-D CRISPR effector.</title>
            <authorList>
                <person name="Cannone G."/>
                <person name="Kompaniiets D."/>
                <person name="Graham S."/>
                <person name="White M.F."/>
                <person name="Spagnolo L."/>
            </authorList>
            <dbReference type="PubMed" id="36843655"/>
            <dbReference type="DOI" id="10.1016/j.crstbi.2023.100098"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (3.50 ANGSTROMS) OF 1-300</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90301">
        <property type="entry name" value="B90301"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923397.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="8BMW">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="M=1-300"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YA1"/>
    <dbReference type="SMR" id="Q97YA1"/>
    <dbReference type="STRING" id="273057.SSO1431"/>
    <dbReference type="PaxDb" id="273057-SSO1431"/>
    <dbReference type="EnsemblBacteria" id="AAK41665">
        <property type="protein sequence ID" value="AAK41665"/>
        <property type="gene ID" value="SSO1431"/>
    </dbReference>
    <dbReference type="GeneID" id="1454444"/>
    <dbReference type="KEGG" id="sso:SSO1431"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1458"/>
    <dbReference type="eggNOG" id="arCOG06024">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_914057_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YA1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="5" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="220"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="8BMW"/>
        </source>
    </evidence>
    <sequence length="304" mass="34876" checksum="AC1E814C306C7EA4" modified="2001-10-01" version="1">MILIKLKVKGYITTQMRRIRNYYFSITNYIPSTTLRGAILAEYYNQTGKIDENFYVSPAYPIKTAPAHYFSPAKERKGDEFIEVKRILEKKEKEFEANKPIEEIMKLEIDGKHPKPKIGSLITYEGETDKENKYREFSSKSIIQMHVAIDKTSISSYKGMLFAYEYKEFDEMWAIASDSEVIDTVKRIKIGRGKNRGNKVVDVEKVREVSLDQSKGLLYCLSPCIGSLFGKTFFKAKYIIGDKSIYSGWFTVDSFSGQKPVFETLREGSLVYVESFSNEKSLMPAGLNFMLRISDLSSILNKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-11-27" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YF9</accession>
    <name>Q97YF9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1365</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90292">
        <property type="entry name" value="H90292"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923356.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YF9"/>
    <dbReference type="PaxDb" id="273057-SSO1365"/>
    <dbReference type="EnsemblBacteria" id="AAK41599">
        <property type="protein sequence ID" value="AAK41599"/>
        <property type="gene ID" value="SSO1365"/>
    </dbReference>
    <dbReference type="GeneID" id="71027434"/>
    <dbReference type="KEGG" id="sso:SSO1365"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1370"/>
    <dbReference type="eggNOG" id="arCOG07237">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_656592_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YF9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18708">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="49820" checksum="F6691E94A2A965D1" modified="2001-10-01" version="1">MEKLATLGSDKSVTTINAIMTELLTDLKPTQIIILRESPPHKRIEDNLKKALSYLGINVEVKDIVIGEGVKTWREKISEYDPDVLDITPGRKYMALTTANYSKAREVRYVYLKNENEGYRVFGYVPFNEIKIFNMRNGEEIKFRHLPLTTAGDELDSELDIESLTALYNILSLHGEVKIKLGDNNYIDDQKGDEFIELCKTRSGMRTFREEEKILDIIKKEDALFVADTNVYISLGNRIKRLFYDREKGFRLIPSTSVYRELKSKTQGTQKDPNLIKFILGLQTFKEVHLPSISQLEDRLKNQSYGDVELKKEVNRLKTAIPTNVYLVTMDQSLGLSASTEIKPIVLKTTTYHDRFDMGEYLNCLSYYHQYYNYKNGFNAKINKQLILELNGNNVVKITNTLTGEPKVDIKTLDKRYNYAKVLEVLKNTISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YR8</accession>
    <name>Q97YR8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90279">
        <property type="entry name" value="C90279"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990179.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YR8"/>
    <dbReference type="SMR" id="Q97YR8"/>
    <dbReference type="PaxDb" id="273057-SSO1251"/>
    <dbReference type="DNASU" id="1454269"/>
    <dbReference type="EnsemblBacteria" id="AAK41490">
        <property type="protein sequence ID" value="AAK41490"/>
        <property type="gene ID" value="SSO1251"/>
    </dbReference>
    <dbReference type="GeneID" id="1454269"/>
    <dbReference type="KEGG" id="sso:SSO1251"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1245"/>
    <dbReference type="eggNOG" id="arCOG01205">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159724_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YR8"/>
    <dbReference type="PhylomeDB" id="Q97YR8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37030">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37030:SF1">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="25"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="113" mass="13472" checksum="EFF0DD7857E2C6EF" modified="2001-10-01" version="1">MSIIEVLEENKRLRDNYIKNLNFYLDRIKEVVKNIDPSTKIILFGSYIRGNFRPDSDIDVLIITNVSNEFDRLKIYHEVNKAIGNPNPFEIHVINEEEYVSWYSKFLDVYKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z25</accession>
    <name>Q97Z25_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF8155 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90264">
        <property type="entry name" value="E90264"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989748.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z25"/>
    <dbReference type="STRING" id="273057.SSO1116"/>
    <dbReference type="PaxDb" id="273057-SSO1116"/>
    <dbReference type="EnsemblBacteria" id="AAK41372">
        <property type="protein sequence ID" value="AAK41372"/>
        <property type="gene ID" value="SSO1116"/>
    </dbReference>
    <dbReference type="GeneID" id="1454151"/>
    <dbReference type="KEGG" id="sso:SSO1116"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1112"/>
    <dbReference type="eggNOG" id="arCOG02979">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1010535_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z25"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="entry name" value="Glucose Permease (Domain IIA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058468">
        <property type="entry name" value="DUF8155_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26482">
        <property type="entry name" value="DUF8155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8155" evidence="1">
        <location>
            <begin position="9"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="279" mass="31757" checksum="9DBF843F5E1416B1" modified="2001-10-01" version="1">MFIKDGSLISYFSSGFPSHVRVKAIDISSPDYEVFYSPVKGEVVDIVRFEIGRPNRFSTINYDYMILINSENQRIIKVLHVLPWVEKGEYVKEGRVIGEFLQTPYTGGDFPHAHIEGITIRFPKISNYRDSKIGIVHKKDKEFFDVIIKDFAEAGKLRGLGCCGGLLNASLPYACYGGIIGGFTGQLSLYDLNLGYLAVRRKTYLLFEGRKNLLRNWEEDASFKVLVNKPICGFAFMEIVLSHNGYPMIRFFLNNVNLSEGDEIDISEFVRDRLGSKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z27</accession>
    <name>Q97Z27_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1114</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90264">
        <property type="entry name" value="C90264"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989746.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z27"/>
    <dbReference type="STRING" id="273057.SSO1114"/>
    <dbReference type="PaxDb" id="273057-SSO1114"/>
    <dbReference type="EnsemblBacteria" id="AAK41370">
        <property type="protein sequence ID" value="AAK41370"/>
        <property type="gene ID" value="SSO1114"/>
    </dbReference>
    <dbReference type="GeneID" id="1454149"/>
    <dbReference type="KEGG" id="sso:SSO1114"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1110"/>
    <dbReference type="eggNOG" id="arCOG05825">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067819_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z27"/>
    <dbReference type="PhylomeDB" id="Q97Z27"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288">
        <property type="entry name" value="BIOTIN SYNTHASE-RELATED PROTEIN, RADICAL SAM SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288:SF2">
        <property type="entry name" value="RADICAL SAM CORE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01113">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="18"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="301" mass="33752" checksum="23B7FBD88E753564" modified="2001-10-01" version="1">MRPLFLYAPNLKRYESEFLDSRKGWKSISVTGTYCAFNCKHCGRRVLESMIDGSTQGKIEKEIMEAISRGDEGIILSGGSTSRGDVPIWRYSNLLKRYSDRLTIIAHTGVVKNEEIARKFKESGVKIALLDMVSDNDAIKDILGQPFTVDDYLNSFKYLKKVNIKIVPHVILGLSKKGLEGDLESIRLLQEVNPDALIIVGLMPLVGTQMNASRPPTPEEIIVALRTARDTFPNIPINLGCARPRGKSYLEVEKFAVDYDIDAIAFPEDETYEYAKSKREIFLSNACCGNIVFDIFKVITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z53</accession>
    <name>Q97Z53_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, marR family, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1082</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90260">
        <property type="entry name" value="H90260"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z53"/>
    <dbReference type="FunCoup" id="Q97Z53">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1082"/>
    <dbReference type="EnsemblBacteria" id="AAK41343">
        <property type="protein sequence ID" value="AAK41343"/>
        <property type="gene ID" value="SSO1082"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1082"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1081"/>
    <dbReference type="eggNOG" id="arCOG03181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_18_6_2"/>
    <dbReference type="InParanoid" id="Q97Z53"/>
    <dbReference type="PhylomeDB" id="Q97Z53"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42756">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42756:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR OF EMRAB OPERON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH marR-type" evidence="4">
        <location>
            <begin position="22"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="154" mass="18268" checksum="ED442CF0E00A747B" modified="2001-10-01" version="1">MMMKNFIVYKVRYLTMILATKYSEVWDLITGLTRKINKDTDKALEQIGLSYFEFKVMCALEEEGKVPMSRIAEKYMLTKAGLTSIIDRLEEKELVRRVRSESDRRVIYVELTEKGRERLMESRKIFLNVLSSFLSKLSEDEIRELERLFSKLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z81</accession>
    <name>Q97Z81_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl44E</name>
        <name evidence="4" type="synonym">rpl44e</name>
        <name evidence="5" type="ordered locus">SSO7111</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90257">
        <property type="entry name" value="A90257"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989178.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z81"/>
    <dbReference type="FunCoup" id="Q97Z81">
        <property type="interactions" value="282"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO7111"/>
    <dbReference type="PaxDb" id="273057-SSO7111"/>
    <dbReference type="EnsemblBacteria" id="AAK41312">
        <property type="protein sequence ID" value="AAK41312"/>
        <property type="gene ID" value="SSO7111"/>
    </dbReference>
    <dbReference type="GeneID" id="15297547"/>
    <dbReference type="KEGG" id="sso:SSO7111"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1044"/>
    <dbReference type="eggNOG" id="arCOG04109">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114645_3_0_2"/>
    <dbReference type="InParanoid" id="Q97Z81"/>
    <dbReference type="PhylomeDB" id="Q97Z81"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.80:FF:000001">
        <property type="entry name" value="60S ribosomal protein L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01476">
        <property type="entry name" value="Ribosomal_L44e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_eL44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053708">
        <property type="entry name" value="Ribosomal_LSU_eL42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004425">
        <property type="entry name" value="PRK05767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36A/L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="4">
        <location>
            <begin position="11"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="95" mass="10986" checksum="3648E4F8AB93E1A7" modified="2001-10-01" version="1">MKVPKVISTYCPKCKTHTDHSVSLYKSGKRRNLAEGQRRYERKNIGYGSKRKPEQKRFAKVTKKQTLVLKCSKCGYTIVKEGIRVKKLELVEVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZB3</accession>
    <name>Q97ZB3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="4">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ilvB-3</name>
        <name evidence="11" type="ordered locus">SSO1012</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90253">
        <property type="entry name" value="A90253"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989226.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZB3"/>
    <dbReference type="FunCoup" id="Q97ZB3">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1012"/>
    <dbReference type="PaxDb" id="273057-SSO1012"/>
    <dbReference type="EnsemblBacteria" id="AAK41280">
        <property type="protein sequence ID" value="AAK41280"/>
        <property type="gene ID" value="SSO1012"/>
    </dbReference>
    <dbReference type="GeneID" id="7809082"/>
    <dbReference type="KEGG" id="sso:SSO1012"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1006"/>
    <dbReference type="eggNOG" id="arCOG01999">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_0_2"/>
    <dbReference type="InParanoid" id="Q97ZB3"/>
    <dbReference type="PhylomeDB" id="Q97ZB3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052737">
        <property type="term" value="F:pyruvate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02014">
        <property type="entry name" value="TPP_POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07039">
        <property type="entry name" value="TPP_PYR_POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000007">
        <property type="entry name" value="Acetolactate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047211">
        <property type="entry name" value="POXB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047212">
        <property type="entry name" value="TPP_POXB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047210">
        <property type="entry name" value="TPP_PYR_POXB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005040">
        <property type="entry name" value="PRK06457.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42981">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE [UBIQUINONE]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42981:SF2">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE [UBIQUINONE]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="10">
        <location>
            <begin position="4"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="8">
        <location>
            <begin position="187"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="9">
        <location>
            <begin position="369"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK41280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="549" mass="60754" checksum="AA9E3C6704965073" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="145" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZC0</accession>
    <name>Q97ZC0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sugar ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1003</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90252">
        <property type="entry name" value="B90252"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989235.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZC0"/>
    <dbReference type="FunCoup" id="Q97ZC0">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1003"/>
    <dbReference type="TCDB" id="3.A.1.1.15">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1003"/>
    <dbReference type="EnsemblBacteria" id="AAK41273">
        <property type="protein sequence ID" value="AAK41273"/>
        <property type="gene ID" value="SSO1003"/>
    </dbReference>
    <dbReference type="GeneID" id="1455240"/>
    <dbReference type="KEGG" id="sso:SSO1003"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.999"/>
    <dbReference type="eggNOG" id="arCOG00175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_2"/>
    <dbReference type="InParanoid" id="Q97ZC0"/>
    <dbReference type="PhylomeDB" id="Q97ZC0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055052">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03301">
        <property type="entry name" value="ABC_MalK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000042">
        <property type="entry name" value="Maltose/maltodextrin ABC transporter, ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015855">
        <property type="entry name" value="ABC_transpr_MalK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047641">
        <property type="entry name" value="ABC_transpr_MalK/UgpC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875">
        <property type="entry name" value="MALTODEXTRIN IMPORT ATP-BINDING PROTEIN MSMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875:SF15">
        <property type="entry name" value="TREHALOSE IMPORT ATP-BINDING PROTEIN SUGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="MOP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="3"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="324" mass="36801" checksum="2113645FDC6299BD" modified="2001-10-01" version="1">MTVELIDIVKKYGKNIVINGITEKIETGEFFVILGPSGEGKSTLLKILAGIEKLDKGKIIADGADITDKPPEKRNVAMVFQNYALYPNMSVRDNIAFPLKMRGMKKEEIIERVEKAAKLLGISEILDKKVTQISGGQQQRVALARAIVRNPSYFLLDEPLSNLDARVRTTARGELKRIQKELKGTFIYVTHDQKEALSLADRIAILHKGKFEQVSDPKTLYEYPKTKWVAQFVGEFPMNFLPGELMKEKAQEIGFRPEWVEVGKGNLSCMVESVEASGESRYLICNFKNNNITILSQEFYDVGQEVRFEIIKYRKYNDGRLVQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZC3</accession>
    <name>Q97ZC3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0999</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90251">
        <property type="entry name" value="G90251"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989239.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZC3"/>
    <dbReference type="FunCoup" id="Q97ZC3">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0999"/>
    <dbReference type="TCDB" id="3.A.1.1.15">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO0999"/>
    <dbReference type="EnsemblBacteria" id="AAK41270">
        <property type="protein sequence ID" value="AAK41270"/>
        <property type="gene ID" value="SSO0999"/>
    </dbReference>
    <dbReference type="GeneID" id="1455237"/>
    <dbReference type="KEGG" id="sso:SSO0999"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.996"/>
    <dbReference type="eggNOG" id="arCOG00153">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_582192_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZC3"/>
    <dbReference type="PhylomeDB" id="Q97ZC3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050490">
        <property type="entry name" value="Bact_solute-bd_prot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43649">
        <property type="entry name" value="ARABINOSE-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43649:SF29">
        <property type="entry name" value="OSMOPROTECTIVE COMPOUNDS-BINDING PROTEIN GGTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="477" mass="53283" checksum="373F86E1FF96AF1F" modified="2001-10-01" version="1">MSRSDKFSNKEKMRRGLSTTTIIGIVVAIVIIVIGAVAAVTLLSHKPSQVVSTTSPSTSQSATSTSPSQVITITYFDDLSPSEANITQKIIIPQFEATHPNIKINYVDESADDIVKSVVELVKSGNVGPVIIGEDNLVIGELLNGNYLMNLTPYVNQILQNVSLIPSMQSLVQYEQKVYHGTYFIPLRANIPLVWYNASLFRQLGLASPPENWSQLLQYAKIIYDKTGVKPIMFQGHGGASTYTELYQWMVQAGGNPFLFNDSGDVLAFEYLYNLSQYFNLEYIHGYWGSYKGLIDGSYYLIDYQWPYIYSVMASEGVNMSNIGFYPGPTGPVNGDHLVGGDVLAIPKGATHIDALIEFAKFLLSPQVQREFIIYLSWPAVNQQAYQNLPSNISSLYKAEEEALQNAFFREPVPWITVWGQIADNVFNQIIVNHAPYSQIPQILSQANKEMYQYLLQNYNVTVAQEYEEGVFGPLYG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-30" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZC5</accession>
    <name>NADB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">L-aspartate oxidase</fullName>
            <shortName evidence="1">LASPO</shortName>
            <ecNumber evidence="1">1.4.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Quinolinate synthase B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">nadB</name>
        <name type="ordered locus">SSO0997</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidation of L-aspartate to iminoaspartate, the first step in the de novo biosynthesis of NAD(+).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-aspartate + O2 = iminosuccinate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:25876"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="1.4.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:25877"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK41268" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="1.4.3.16" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41268.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90251">
        <property type="entry name" value="E90251"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989244.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZC5"/>
    <dbReference type="SMR" id="Q97ZC5"/>
    <dbReference type="FunCoup" id="Q97ZC5">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0997"/>
    <dbReference type="PaxDb" id="273057-SSO0997"/>
    <dbReference type="EnsemblBacteria" id="AAK41268">
        <property type="protein sequence ID" value="AAK41268"/>
        <property type="gene ID" value="SSO0997"/>
    </dbReference>
    <dbReference type="GeneID" id="1455235"/>
    <dbReference type="KEGG" id="sso:SSO0997"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.994"/>
    <dbReference type="eggNOG" id="arCOG00572">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_3_2_2"/>
    <dbReference type="InParanoid" id="Q97ZC5"/>
    <dbReference type="PhylomeDB" id="Q97ZC5"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00326"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008734">
        <property type="term" value="F:L-aspartate oxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034628">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-aspartate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005288">
        <property type="entry name" value="NadB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42716">
        <property type="entry name" value="L-ASPARTATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42716:SF2">
        <property type="entry name" value="L-ASPARTATE OXIDASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184411" description="L-aspartate oxidase">
        <location>
            <begin position="1"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="348"/>
            <end position="349"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="site" description="Important in orienting the L-aspartate substrate" evidence="1">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P10902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="472" mass="52645" checksum="DABAE606463C353D" modified="2002-08-30" version="2">MIYIFGSGLAGLSAAISLHKSGYKVTIISKKINGGSSYWAKGGIAAAVGNDDSPELHKIDTLEVGDGLCDSKTVDYVTREIRYVVSTVEKWGFKFDDDLRLEGGHSKRRILHKTDDTGREITNFLLNLAKKKGINLIEDKLLALKVKDGKVAGFITEKGGSFDAEKVVLATGGYGYLFKFTSNPSTNIGDGIAIAFKAGALVSDTEFVQFHPTVTTFDGQAYLLTETLRGEGAILVNERNERFVFKYDSRGELAPRDVLSRAIYDQYKKGHTVYIDLSPIEDFDRKFPILSNYVKRYGKRLQVFPGVHYTIGGIRVNTRGESNIKGLYAIGEVTDTGLHGANRLASNSLAEDLVYGVNLVRYIDNWEGLSIDDVKEVIEVRLRNSSNRLSLEEIREYNWNYLGIVRNGEGLDKLVKIYESNDTFNDNASLVSLLSAKGALLRTESRGAHYREDYPNKSWEDGKRIYFMVSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZD1</accession>
    <name>Q97ZD1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sugar phosphate nucleotydyl transferase</fullName>
            <ecNumber evidence="4">2.7.7.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0989</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90250">
        <property type="entry name" value="F90250"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZD1"/>
    <dbReference type="STRING" id="273057.SSO0989"/>
    <dbReference type="PaxDb" id="273057-SSO0989"/>
    <dbReference type="EnsemblBacteria" id="AAK41261">
        <property type="protein sequence ID" value="AAK41261"/>
        <property type="gene ID" value="SSO0989"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0989"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.986"/>
    <dbReference type="eggNOG" id="arCOG00666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_2_2"/>
    <dbReference type="InParanoid" id="Q97ZD1"/>
    <dbReference type="PhylomeDB" id="Q97ZD1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010170">
        <property type="term" value="C:glucose-1-phosphate adenylyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008878">
        <property type="term" value="F:glucose-1-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009250">
        <property type="term" value="P:glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="2">
        <location>
            <begin position="12"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="3">
        <location>
            <begin position="309"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="422" mass="48226" checksum="4CAA24FD66E11483" modified="2001-10-01" version="1">MMTKWDISDVKVIIPIGGEATRLRPLTIETSKAAVRLLNRPLIEYTILELAKQGIKEFIFGVKGYVNYRSLFDLFKEGIGFSARYKIKPRVHFKYQPRVDSIGNADSVRINIEYYDINEPIVVVQGDNIFKLDITKALEFHESKSSLMTIVLKKYEGDLSEFGVADTTGDLAIRKFVEKPKRREDAPSDLINTGIYILSPEIRKIFKSNDVKEMYKMGKMDFGKDIIPYLINKGYPVYGYPMKEIWFDIGTPERYLDAMVTLLHTLSDSEIGGIRIDPNRRIFVQGTSPDSRKRRREIQRKFKKGLIKIEGDALIGRHCQIGDDTYIEESNIDNFTMIGKGVKIVRSAIMDRGYIGDNVHIENSIIARHVEIRSNNEKPVRIINSVIADDVIVGEGTEIINSRIYPHKFINAGSRIHDTILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZN0</accession>
    <name>Q97ZN0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO6687</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90236">
        <property type="entry name" value="F90236"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923144.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZN0"/>
    <dbReference type="STRING" id="273057.SSO6687"/>
    <dbReference type="PaxDb" id="273057-SSO6687"/>
    <dbReference type="EnsemblBacteria" id="AAK41149">
        <property type="protein sequence ID" value="AAK41149"/>
        <property type="gene ID" value="SSO6687"/>
    </dbReference>
    <dbReference type="GeneID" id="84058713"/>
    <dbReference type="KEGG" id="sso:SSO6687"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.870"/>
    <dbReference type="eggNOG" id="arCOG05983">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_194984_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZN0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="67" mass="7707" checksum="835449EE4F84453B" modified="2001-10-01" version="1">MKKRVEILIVEDYSGNPKINEQEIKKSLSHIGDDIVITRLHLPQWTENKDESDLVGVHVIVREVAET</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZR7</accession>
    <name>Q97ZR7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Second ORF in transposon ISC1173</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO0511</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Absolutely required for transposition of IS1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase 27 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99197">
        <property type="entry name" value="A99197"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZR7"/>
    <dbReference type="STRING" id="273057.SSO0511"/>
    <dbReference type="PaxDb" id="273057-SSO0511"/>
    <dbReference type="EnsemblBacteria" id="AAK40832">
        <property type="protein sequence ID" value="AAK40832"/>
        <property type="gene ID" value="SSO0511"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0511"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.508"/>
    <dbReference type="eggNOG" id="arCOG02133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076276_0_1_2"/>
    <dbReference type="InParanoid" id="Q97ZR7"/>
    <dbReference type="PhylomeDB" id="Q97ZR7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005063">
        <property type="entry name" value="Transposase_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051354">
        <property type="entry name" value="Transposase_27_IS1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033558">
        <property type="entry name" value="transpos_IS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293:SF1">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03400">
        <property type="entry name" value="DDE_Tnp_IS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="269" mass="31520" checksum="B4A7829DC01A4C3D" modified="2001-10-01" version="1">MVIPKLKALILKSFYGPLELPGFSDKCYSDRVIKNGSSRGKSKYKCKVCGRTFYRTASHRMSREQRERILREYTNRMSLRGISKVEGRPLTTVYSSVKRAALKAYVDLSILQAQLKAFRSKFAVLDESWTYVRVRRGPRRQNLWIWNALIDGVPFFVTGDRDYNTFRLLWLSLPKCEVHYTDDYPIYQVLYHHVVGKKYTHTVESYNSFCRSHLVRLARDTKAVNRSRAMIDYSLDLLNVMYPQAFTDEKTPLNLAYLSGVQYIRDHLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-05-10" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZY7</accession>
    <accession>E0A3F3</accession>
    <name>RS27A_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">30S ribosomal protein S27ae</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 4" type="primary">rps27ae</name>
        <name type="ordered locus">SSO5844</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2010-05" db="EMBL/GenBank/DDBJ databases">
            <title>An update of the SSO5844-SSO0434-SSO0433 genomic region of the archaea Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Hecker A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5 6 7" key="3">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <dbReference type="EMBL" id="HM370296">
        <property type="protein sequence ID" value="ADK88909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90187">
        <property type="entry name" value="H90187"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922972.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="Y=1-75"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="Y=1-75"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="Y=1-75"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="Y=1-75"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="Y=1-75"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="Y=1-75"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="Y=1-75"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="Y=1-75"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q97ZY7"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q97ZY7"/>
    <dbReference type="FunCoup" id="Q97ZY7">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO5844"/>
    <dbReference type="PaxDb" id="273057-SSO5844"/>
    <dbReference type="EnsemblBacteria" id="AAK40759">
        <property type="protein sequence ID" value="AAK40759"/>
        <property type="gene ID" value="SSO5844"/>
    </dbReference>
    <dbReference type="GeneID" id="1455569"/>
    <dbReference type="KEGG" id="sso:SSO5844"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.427"/>
    <dbReference type="eggNOG" id="arCOG04183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179743_1_0_2"/>
    <dbReference type="InParanoid" id="Q97ZY7"/>
    <dbReference type="PhylomeDB" id="Q97ZY7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00777">
        <property type="entry name" value="Ribosomal_eS31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_eS31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022845">
        <property type="entry name" value="Ribosomal_eS31_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001669">
        <property type="entry name" value="PRK00432.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000137707" description="Small ribosomal subunit protein eS31">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000312" key="4">
        <source>
            <dbReference type="EMBL" id="ADK88909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="5">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="75" mass="8681" checksum="26E37F21EBAAEEB8" modified="2001-10-01" version="1">MLELNKRKEEAKVAKEQKVKAIVRTYYVIEGNKVKLKNKKCPRCGSIMAHHLKPNERWSCGKCGYTEFIGASKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="138" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZY9</accession>
    <accession>E0A3F5</accession>
    <name>Q97ZY9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">SSO0433</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">P2</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2010-05" db="EMBL/GenBank/DDBJ databases">
            <title>An update of the SSO5844-SSO0434-SSO0433 genomic region of the archaea Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Hecker A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="HM370296">
        <property type="protein sequence ID" value="ADK88911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90187">
        <property type="entry name" value="F90187"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988744.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZY9"/>
    <dbReference type="FunCoup" id="Q97ZY9">
        <property type="interactions" value="165"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0433"/>
    <dbReference type="PaxDb" id="273057-SSO0433"/>
    <dbReference type="EnsemblBacteria" id="AAK40757">
        <property type="protein sequence ID" value="AAK40757"/>
        <property type="gene ID" value="SSO0433"/>
    </dbReference>
    <dbReference type="GeneID" id="1455567"/>
    <dbReference type="KEGG" id="sso:SSO0433"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.425"/>
    <dbReference type="eggNOG" id="arCOG01185">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063953_2_0_2"/>
    <dbReference type="BRENDA" id="2.7.11.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000201">
        <property type="entry name" value="TP53-regulating kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011460">
        <property type="entry name" value="PRK14879.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011463">
        <property type="entry name" value="PRK14879.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209:SF0">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TP53RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209">
        <property type="entry name" value="NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAK40757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADK88911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="223" mass="25984" checksum="C3EDB9ADD27E5502" modified="2001-10-01" version="1">MEKLRLIKRGAESNIYEGYFLGIHAIFKQRIKKSYRNPELDHKINYERTILEAKIIYTALKNDVNVPAVLFIDPNNYLLVIEYIEGEIVKDIINTNNPTQLLPNIGKRIGELTGKLHNIGIAHGDLTTNNLILSSTNDDIFIIDFGLSRRTQDEEDFATDLHVFLRSLESVHSDFKDIIYDAFIEGYRKIMGRKTDEILELVKDIRMRGRYVEERRKNRSINE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2026-01-28" version="145" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZZ8</accession>
    <name>RGYR1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12">Reverse gyrase 1</fullName>
            <ecNumber evidence="2 9 10">5.6.2.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14 15 16">TopR1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rgy1</name>
        <name evidence="11 13" type="synonym">topR-1</name>
        <name type="ordered locus">SSO0420</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="J. Biol. Chem." volume="279" first="33192" last="33198">
            <title>Reverse gyrase recruitment to DNA after UV light irradiation in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Napoli A."/>
                <person name="Valenti A."/>
                <person name="Salerno V."/>
                <person name="Nadal M."/>
                <person name="Garnier F."/>
                <person name="Rossi M."/>
                <person name="Ciaramella M."/>
            </authorList>
            <dbReference type="PubMed" id="15190074"/>
            <dbReference type="DOI" id="10.1074/jbc.m402619200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>REACTION MECHANISM</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2006" name="Nucleic Acids Res." volume="34" first="2098" last="2108">
            <title>Selective degradation of reverse gyrase and DNA fragmentation induced by alkylating agent in the archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Valenti A."/>
                <person name="Napoli A."/>
                <person name="Ferrara M.C."/>
                <person name="Nadal M."/>
                <person name="Rossi M."/>
                <person name="Ciaramella M."/>
            </authorList>
            <dbReference type="PubMed" id="16617150"/>
            <dbReference type="DOI" id="10.1093/nar/gkl115"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>INDUCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2008" name="Extremophiles" volume="12" first="799" last="809">
            <title>Transcriptional analysis of the two reverse gyrase encoding genes of Sulfolobus solfataricus P2 in relation to the growth phases and temperature conditions.</title>
            <authorList>
                <person name="Garnier F."/>
                <person name="Nadal M."/>
            </authorList>
            <dbReference type="PubMed" id="18777006"/>
            <dbReference type="DOI" id="10.1007/s00792-008-0186-2"/>
        </citation>
        <scope>INDUCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2011" name="J. Mol. Biol." volume="408" first="839" last="849">
            <title>TopR2, the second reverse gyrase of Sulfolobus solfataricus, exhibits unusual properties.</title>
            <authorList>
                <person name="Bizard A."/>
                <person name="Garnier F."/>
                <person name="Nadal M."/>
            </authorList>
            <dbReference type="PubMed" id="21435345"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2011.03.030"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>REACTION MECHANISM</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2010" name="J. Biol. Chem." volume="285" first="36532" last="36541">
            <title>The archaeal topoisomerase reverse gyrase is a helix-destabilizing protein that unwinds four-way DNA junctions.</title>
            <authorList>
                <person name="Valenti A."/>
                <person name="Perugino G."/>
                <person name="Varriale A."/>
                <person name="D'Auria S."/>
                <person name="Rossi M."/>
                <person name="Ciaramella M."/>
            </authorList>
            <dbReference type="PubMed" id="20851892"/>
            <dbReference type="DOI" id="10.1074/jbc.m110.169029"/>
        </citation>
        <scope>FUNCTION AS A HOLLIDAY JUNCTION RESOLVASE</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>MUTAGENESIS OF LYS-116 AND TYR-965</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2020" name="Mol. Microbiol." volume="113" first="356" last="368">
            <title>The reverse gyrase TopR1 is responsible for the homeostatic control of DNA supercoiling in the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Couturier M."/>
                <person name="Gadelle D."/>
                <person name="Forterre P."/>
                <person name="Nadal M."/>
                <person name="Garnier F."/>
            </authorList>
            <dbReference type="PubMed" id="31713907"/>
            <dbReference type="DOI" id="10.1111/mmi.14424"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PROTEIN ABUNDANCE</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>INDUCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9 10 17 18">Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process (Probable) (PubMed:15190074, PubMed:16617150, PubMed:21435345, PubMed:31713907). Increases the linking number in steps of +1 (PubMed:21435345). Involved in homeostatic control of DNA topology in balance with type II topoisomerase 6 (TopoVI); levels of TopoVI are constant at 80 and 88 degrees Celsius and TopoVI is probably less active at 88 degrees (characterized enzyme is from S.shibatae B12), so reverse gyrase mediates most of the fine-tuning of DNA topology (PubMed:31713907). Changes the DNA linking number step-by-step in a distributive manner. At low protein to DNA ratios mostly relaxes negatively supercoiled substrate, as ratios rise more positive supercoils are introduced. At 90 degrees Celsius introduces 19 positive supercoils into pTZ18R DNA (probably 2860 bp), less than TopR2 (PubMed:21435345). Relaxes negatively supercoiled DNA in the absence of ATP (PubMed:21435345). It cleaves transiently a single DNA strand and remains covalently bound to the 5' DNA end through a tyrosine residue (Probable) (PubMed:15190074). May be involved in DNA damage response (Probable) (PubMed:15190074). Its activity is inhibited by the DNA-binding protein 7d (Sso7d), suggesting that the Sso7d activity might counteract the overwinding effect of reverse gyrase.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Resolves 4-way Holliday junctions (HJ) with 20 bases in each arm in vitro, distorting the junction (PubMed:20851892). Very high protein levels are required, but total enzyme content of the cell (there are 2 reverse gyrases in this organism) is estimated to be 20-200 molecules/cell (PubMed:20851892). HJ resolution does not require either ATPase activity or the active tyrosine (PubMed:20851892). The individual domains do not resolve HJs but do so when mixed (PubMed:20851892). Also unwinds a fork substrate (PubMed:20851892).</text>
    </comment>
    <comment type="function">
        <text evidence="10 17 18">There are 2 genes for this protein in the cell. During exponential growth this is the less expressed isoform (about 52 molecules per cell at 80 degrees Celsius, about 28 molecules at 88 degrees Celsius); this isoform is more active at higher temperature (PubMed:31713907). Grows actively at both 80 and 88 degrees Celsius; survives a long exposure at 45 degrees Celsius without DNA replication or cell division occurring (PubMed:31713907). Experiments using whole cell extracts do not distinguish which isoform is present, the results are probably a mixture of the two forms (Probable) (PubMed:15190074, PubMed:16617150).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9 17">At least one of the two reverse gyrase proteins is inhibited by actinomycin D (Probable) (PubMed:15190074). Highly sensitive to NaCl concentrations, maximal positive supercoiling is observed with 10 mM NaCl; as NaCl rises, supercoiling decreases (PubMed:21435345). At 300 mM NaCl relaxes but does not introduce positive supercoils into negatively supercoiled substrate, at 400 mM NaCl does not relax DNA (PubMed:21435345).</text>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text evidence="8 9 10">Optimum temperature for reverse gyrase activity is 90 degrees Celsius, at lower temperatures fewer positive supercoils are introduced, under 60 degrees Celsius only relaxation of negative supercoils is seen in vitro (PubMed:21435345). Active at 80 degrees, has higher activity at 88 degrees Celsius in vitro (PubMed:31713907). Holliday junction resolution is optimal in vitro at 55 degrees Celsius, at 37 degrees Celsius no resolution occurs (PubMed:20851892).</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="17">Upon UV irradiation up to 60% of the reverse gyrase activity becomes associated with DNA in vivo (PubMed:15190074).</text>
    </comment>
    <comment type="induction">
        <text evidence="6 7 10 18">Protein levels increase during a shift from 88 to 80 degrees Celsius and decrease upon a shift from 80 to 88 degrees Celsius during exponential growth (at protein level) (PubMed:31713907). Constitutively transcribed during cell growth; no change is seen in the presence of methyl methanesulfonate (MMS) (PubMed:16617150). 0.7 mM MMS reduces the amount of enzyme at a post-translational level via a metalloprotease (at protein level) (Probable) (PubMed:16617150). At 72 degrees Celsius this gene is transcribed at low levels during early and late exponential growth (until about 50 hours), declines as stationary phase starts, disappears by late stationary phase (PubMed:18777006). At 80 degrees Celsius more transcripts accumulate with maximal levels at about 50 hours, declines more slowly than at 72 degrees (PubMed:18777006). Upon a shift from 80 to 72 degrees Celsius this gene is up-regulated within 5 minutes to a maximum increase of 2.4.fold by 30 minutes, then a gradual decline to pre-shift levels (PubMed:18777006). When shifted from 72 to 80 degrees Celsius a slight decline is seen by 30 minutes, transcripts disappear by 24 hours post shift (PubMed:18777006).</text>
    </comment>
    <comment type="domain">
        <text evidence="2">Both the DNA unwinding and positive supercoiling activities require the cooperation of both domains. The cooperative action between the helicase-like and the topoisomerase domains is specific. The helicase-like domain probably does not directly unwind DNA but acts more likely by driving ATP-dependent conformational changes within the whole enzyme, functioning more like a protein motor. The 'latch' region of the N-terminal domain plays a regulatory role in the enzyme, repressing topoisomerase activity in the absence of ATP and therefore preventing the enzyme from acting as an ATP-independent relaxing enzyme; it also helps to coordinate nucleotide hydrolysis by the ATPase domain with the supercoiling activity of the topoisomerase domain.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">This enzyme is the only unique feature of hyperthermophilic bacteria/archaea known and seems to be essential for adaptation to life at high temperatures. It may play a role in stabilization of DNA at high temperatures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the DEAD box helicase family. DDVD subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the type IA topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.-" evidence="2 9 10"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90186">
        <property type="entry name" value="D90186"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988760.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZZ8"/>
    <dbReference type="SMR" id="Q97ZZ8"/>
    <dbReference type="FunCoup" id="Q97ZZ8">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0420"/>
    <dbReference type="PaxDb" id="273057-SSO0420"/>
    <dbReference type="EnsemblBacteria" id="AAK40747">
        <property type="protein sequence ID" value="AAK40747"/>
        <property type="gene ID" value="SSO0420"/>
    </dbReference>
    <dbReference type="GeneID" id="1455557"/>
    <dbReference type="KEGG" id="sso:SSO0420"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.414"/>
    <dbReference type="eggNOG" id="arCOG01526">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002886_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZZ8"/>
    <dbReference type="PhylomeDB" id="Q97ZZ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160097">
        <property type="term" value="F:reverse gyrase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd17924">
        <property type="entry name" value="DDXDc_reverse_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18798">
        <property type="entry name" value="SF2_C_reverse_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03361">
        <property type="entry name" value="TOPRIM_TopoIA_RevGyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.510.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01125">
        <property type="entry name" value="Reverse_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005736">
        <property type="entry name" value="Reverse_gyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034142">
        <property type="entry name" value="TOPRIM_RevGyr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040569">
        <property type="entry name" value="Znf_Rg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01054">
        <property type="entry name" value="rgy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43505">
        <property type="entry name" value="REVERSE GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43505:SF1">
        <property type="entry name" value="REVERSE GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17915">
        <property type="entry name" value="zf_Rg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52037">
        <property type="entry name" value="ZF_RG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52036">
        <property type="entry name" value="ZF_RG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0799">Topoisomerase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000158092" description="Reverse gyrase 1">
        <location>
            <begin position="1"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="97"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="2">
        <location>
            <begin position="619"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="5">
        <location>
            <begin position="801"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="RG N-terminal-type" evidence="3">
        <location>
            <begin position="6"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="RG C-terminal-type; atypical" evidence="4">
        <location>
            <begin position="703"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Topoisomerase I" evidence="2">
        <location>
            <begin position="615"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEAD box" evidence="2">
        <location>
            <begin position="214"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="5">
        <location>
            <position position="965"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="110"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="625"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="706"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="710"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="721"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="724"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="754"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="No change in Holliday junction resolution." evidence="8">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No change in Holliday junction resolution." evidence="8">
        <original>Y</original>
        <variation>F</variation>
        <location>
            <position position="965"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P74759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="16617150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="18777006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="8">
        <source>
            <dbReference type="PubMed" id="20851892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="9">
        <source>
            <dbReference type="PubMed" id="21435345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="10">
        <source>
            <dbReference type="PubMed" id="31713907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="11">
        <source>
            <dbReference type="PubMed" id="11427726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="12">
        <source>
            <dbReference type="PubMed" id="15190074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="13">
        <source>
            <dbReference type="PubMed" id="16617150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="14">
        <source>
            <dbReference type="PubMed" id="20851892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="15">
        <source>
            <dbReference type="PubMed" id="21435345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="16">
        <source>
            <dbReference type="PubMed" id="31713907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="17">
        <source>
            <dbReference type="PubMed" id="15190074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="18">
        <source>
            <dbReference type="PubMed" id="16617150"/>
        </source>
    </evidence>
    <sequence length="1242" mass="141668" checksum="EE4C1990F01F26FB" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q980A1</accession>
    <name>DPCKG_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTP-dependent dephospho-CoA kinase</fullName>
            <ecNumber evidence="1">2.7.1.237</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dephospho-coenzyme A kinase</fullName>
            <shortName evidence="1">DPCK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0416</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the GTP-dependent phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A (CoA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3'-dephospho-CoA + GTP = GDP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61156"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="2.7.1.237"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-dependent DPCK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.237" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90186">
        <property type="entry name" value="A90186"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988765.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980A1"/>
    <dbReference type="SMR" id="Q980A1"/>
    <dbReference type="FunCoup" id="Q980A1">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0416"/>
    <dbReference type="PaxDb" id="273057-SSO0416"/>
    <dbReference type="EnsemblBacteria" id="AAK40744">
        <property type="protein sequence ID" value="AAK40744"/>
        <property type="gene ID" value="SSO0416"/>
    </dbReference>
    <dbReference type="GeneID" id="1455554"/>
    <dbReference type="KEGG" id="sso:SSO0416"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.411"/>
    <dbReference type="eggNOG" id="arCOG04076">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120795_1_0_2"/>
    <dbReference type="InParanoid" id="Q980A1"/>
    <dbReference type="PhylomeDB" id="Q980A1"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00590">
        <property type="entry name" value="Dephospho_CoA_kinase_GTP_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007164">
        <property type="entry name" value="GTP-dep_dephospho-CoA_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732:SF1">
        <property type="entry name" value="GTP-DEPENDENT DEPHOSPHO-COA KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732">
        <property type="entry name" value="UPF0218 PROTEIN TK1697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04019">
        <property type="entry name" value="DUF359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006533">
        <property type="entry name" value="UCP006533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000137619" description="GTP-dependent dephospho-CoA kinase">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00590"/>
        </source>
    </evidence>
    <sequence length="179" mass="20502" checksum="D8FB4F9A33D0B373" modified="2001-10-01" version="1">MEVRNNNKVNLCFSFDNLRNELSRPYGILFINNKIFLEFISKSIQRGSRIITVGDYVSRVLEENGIVPFLEVIDGKTKRTITQNRAITRNKEYKVTNEAGKIRFEIFEIMENILKDREGGVVFVDGEEDLLVIPVTLSANHGDIVIYGQPNAGAVVIIVNEMIRWRVRDILEKAIVKEC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-10-25" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q980B1</accession>
    <name>CBIA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cobyrinate a,c-diamide synthase</fullName>
            <ecNumber evidence="1">6.3.5.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cobyrinic acid a,c-diamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbiA</name>
        <name type="ordered locus">SSO0404</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of cobyrinate, using either L-glutamine or ammonia as the nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cob(II)yrinate + 2 L-glutamine + 2 ATP + 2 H2O = cob(II)yrinate a,c diamide + 2 L-glutamate + 2 ADP + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58537"/>
            <dbReference type="ChEBI" id="CHEBI:58894"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 10/10.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Comprises of two domains. The C-terminal domain contains the binding site for glutamine and catalyzes the hydrolysis of this substrate to glutamate and ammonia. The N-terminal domain is anticipated to bind ATP and cobyrinate and catalyzes the ultimate synthesis of the diamide product. The ammonia produced via the glutaminase domain is probably translocated to the adjacent domain via a molecular tunnel, where it reacts with an activated intermediate.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The a and c carboxylates of cobyrinate are activated for nucleophilic attack via formation of a phosphorylated intermediate by ATP. CbiA catalyzes first the amidation of the c-carboxylate, and then that of the a-carboxylate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CobB/CbiA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.11" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90184">
        <property type="entry name" value="F90184"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988783.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980B1"/>
    <dbReference type="SMR" id="Q980B1"/>
    <dbReference type="FunCoup" id="Q980B1">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0404"/>
    <dbReference type="PaxDb" id="273057-SSO0404"/>
    <dbReference type="DNASU" id="1455543"/>
    <dbReference type="EnsemblBacteria" id="AAK40733">
        <property type="protein sequence ID" value="AAK40733"/>
        <property type="gene ID" value="SSO0404"/>
    </dbReference>
    <dbReference type="GeneID" id="1455543"/>
    <dbReference type="KEGG" id="sso:SSO0404"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.400"/>
    <dbReference type="eggNOG" id="arCOG00106">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022752_2_1_2"/>
    <dbReference type="InParanoid" id="Q980B1"/>
    <dbReference type="PhylomeDB" id="Q980B1"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00231"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042242">
        <property type="term" value="F:cobyrinic acid a,c-diamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05388">
        <property type="entry name" value="CobB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03130">
        <property type="entry name" value="GATase1_CobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00027">
        <property type="entry name" value="CobB_CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004484">
        <property type="entry name" value="CbiA/CobB_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00379">
        <property type="entry name" value="cobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002204">
        <property type="entry name" value="PRK01077.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873">
        <property type="entry name" value="COBYRINATE A,C-DIAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873:SF1">
        <property type="entry name" value="COBYRINATE A,C-DIAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000141280" description="Cobyrinate a,c-diamide synthase">
        <location>
            <begin position="1"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="GATase cobBQ-type" evidence="1">
        <location>
            <begin position="239"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="320"/>
        </location>
    </feature>
    <feature type="site" description="Increases nucleophilicity of active site Cys" evidence="1">
        <location>
            <position position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00027"/>
        </source>
    </evidence>
    <sequence length="434" mass="49346" checksum="20C0BFF167C9CF7C" modified="2001-10-01" version="1">MKRILLSSDRSGSGKTLITSAIMRALSKKYKVRGFKAGPDFIDPGYHKIATGFPSINLDLWMMGKNNVKRSLAKYGKEFDIGIIEGVMGLYDGVDTLYSTYELAKVTKTPIILIINCSNIGSTVGAIVKGLKYYRSDVSIRGVIFNKIASETHYNYCRNAVEDVEVLGYVPFDKNLEIKSRHLGLVTVEDNREVQNLIRYASELVEKYVDLDKIYEMASDEDLEIDLPEDNESNGVKRKMAIAYDPAFSFYYQENLDILKNKYELEFFSPLNNEYVEDAEAIYIGGGYPELHLNELEKSTRTKKWLKNMSYAGVKIYAECGGLMYLSKNLIDENNKNHSMTGIFDIDIKTKDKLTIGYTELEAVKENFIVNKNNVVRGHEFHVSKPISVNEKEFVFKVRIGKGIINKLDGVKSNNTVASYSHLHFSNFQLRIVF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q980B5</accession>
    <name>RISB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="1">DMRL synthase</shortName>
            <shortName evidence="1">LS</shortName>
            <shortName evidence="1">Lumazine synthase</shortName>
            <ecNumber evidence="1">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ribH</name>
        <name type="ordered locus">SSO0400</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90184">
        <property type="entry name" value="B90184"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988789.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980B5"/>
    <dbReference type="SMR" id="Q980B5"/>
    <dbReference type="FunCoup" id="Q980B5">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0400"/>
    <dbReference type="PaxDb" id="273057-SSO0400"/>
    <dbReference type="EnsemblBacteria" id="AAK40729">
        <property type="protein sequence ID" value="AAK40729"/>
        <property type="gene ID" value="SSO0400"/>
    </dbReference>
    <dbReference type="GeneID" id="1455539"/>
    <dbReference type="GeneID" id="44129381"/>
    <dbReference type="KEGG" id="sso:SSO0400"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.396"/>
    <dbReference type="eggNOG" id="arCOG01323">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089358_3_1_2"/>
    <dbReference type="InParanoid" id="Q980B5"/>
    <dbReference type="PhylomeDB" id="Q980B5"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09211">
        <property type="entry name" value="Lumazine_synthase_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.960:FF:000003">
        <property type="entry name" value="6,7-dimethyl-8-ribityllumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="entry name" value="Lumazine/riboflavin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058:SF0">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE DMRL SYNTHASE LUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="Lumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000134853" description="6,7-dimethyl-8-ribityllumazine synthase">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="47"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="71"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="76"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00178"/>
        </source>
    </evidence>
    <sequence length="154" mass="17247" checksum="ADFA9E6A2C723210" modified="2001-10-01" version="1">MQEKSIRLGIVVAEFNYDITQLMLQKALSHARFLNVEVKVVIKVPGTFDMPLAIKKLLEKDFIDAVVTLGAVIKGETKHDELVASQTARKIVDLSTEFNKPVTLGIIGHGATHEQAVERIEEYATRAVEAAIKLVQRTRKLDELKEIKETVIIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2025-02-05" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q980C5</accession>
    <name>TMCA1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Met) cytidine acetyltransferase TmcA 1</fullName>
            <ecNumber evidence="1 2">2.3.1.193</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tmcA1</name>
        <name evidence="4" type="ordered locus">SSO0391</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2020" name="Nature" volume="583" first="638" last="643">
            <title>Dynamic RNA acetylation revealed by quantitative cross-evolutionary mapping.</title>
            <authorList>
                <person name="Sas-Chen A."/>
                <person name="Thomas J.M."/>
                <person name="Matzov D."/>
                <person name="Taoka M."/>
                <person name="Nance K.D."/>
                <person name="Nir R."/>
                <person name="Bryson K.M."/>
                <person name="Shachar R."/>
                <person name="Liman G.L.S."/>
                <person name="Burkhart B.W."/>
                <person name="Gamage S.T."/>
                <person name="Nobe Y."/>
                <person name="Briney C.A."/>
                <person name="Levy M.J."/>
                <person name="Fuchs R.T."/>
                <person name="Robb G.B."/>
                <person name="Hartmann J."/>
                <person name="Sharma S."/>
                <person name="Lin Q."/>
                <person name="Florens L."/>
                <person name="Washburn M.P."/>
                <person name="Isobe T."/>
                <person name="Santangelo T.J."/>
                <person name="Shalev-Benami M."/>
                <person name="Meier J.L."/>
                <person name="Schwartz S."/>
            </authorList>
            <dbReference type="PubMed" id="32555463"/>
            <dbReference type="DOI" id="10.1038/s41586-020-2418-2"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP).</text>
    </comment>
    <comment type="function">
        <text evidence="2 3">Catalyzes the formation of 41 N(4)-acetylcytidine (ac(4)C) sites in RNA, almost always on the middle C of a CCG motif (PubMed:32555463). Modifications are found mostly in tRNA, with small amounts found in rRNA and mRNA (PubMed:32555463). Four ac(4)C sites (cytidine 1466, 1467, 1477 and 1478) were detected in 16S rRNA, presumably generated by TmcA1 and/or TmcA2 (PubMed:39753558).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>cytidine(34) in elongator tRNA(Met) + acetyl-CoA + ATP + H2O = N(4)-acetylcytidine(34) in elongator tRNA(Met) + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43788"/>
            <dbReference type="Rhea" id="RHEA-COMP:10693"/>
            <dbReference type="Rhea" id="RHEA-COMP:10694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.3.1.193"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a cytidine in RNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in RNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:82211"/>
            <dbReference type="Rhea" id="RHEA-COMP:15704"/>
            <dbReference type="Rhea" id="RHEA-COMP:19834"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a cytidine in tRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in tRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53876"/>
            <dbReference type="Rhea" id="RHEA-COMP:13670"/>
            <dbReference type="Rhea" id="RHEA-COMP:13671"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a cytidine in mRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in mRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:58480"/>
            <dbReference type="Rhea" id="RHEA-COMP:15145"/>
            <dbReference type="Rhea" id="RHEA-COMP:15146"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TmcA family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.193" evidence="1 2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90182">
        <property type="entry name" value="G90182"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980C5"/>
    <dbReference type="SMR" id="Q980C5"/>
    <dbReference type="FunCoup" id="Q980C5">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0391"/>
    <dbReference type="PaxDb" id="273057-SSO0391"/>
    <dbReference type="EnsemblBacteria" id="AAK40718">
        <property type="protein sequence ID" value="AAK40718"/>
        <property type="gene ID" value="SSO0391"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0391"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.385"/>
    <dbReference type="eggNOG" id="arCOG01951">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004652_1_0_2"/>
    <dbReference type="InParanoid" id="Q980C5"/>
    <dbReference type="PhylomeDB" id="Q980C5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990883">
        <property type="term" value="F:18S rRNA cytidine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106162">
        <property type="term" value="F:mRNA N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051392">
        <property type="term" value="F:tRNA cytidine N4-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904812">
        <property type="term" value="P:rRNA acetylation involved in maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051391">
        <property type="term" value="P:tRNA acetylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000140">
        <property type="entry name" value="tRNA(Met) cytidine acetyltransferase TmcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01886">
        <property type="entry name" value="tRNA_acetyltr_TmcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007807">
        <property type="entry name" value="TcmA/NAT10_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032672">
        <property type="entry name" value="TmcA/NAT10/Kre33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013562">
        <property type="entry name" value="TmcA/NAT10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024914">
        <property type="entry name" value="tRNA_acetyltr_TmcA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925">
        <property type="entry name" value="N-ACETYLTRANSFERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925:SF5">
        <property type="entry name" value="RNA CYTIDINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13718">
        <property type="entry name" value="GNAT_acetyltr_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05127">
        <property type="entry name" value="NAT10_TcmA_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08351">
        <property type="entry name" value="TmcA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000461793" description="tRNA(Met) cytidine acetyltransferase TmcA 1">
        <location>
            <begin position="1"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="421"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="532"/>
            <end position="534"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2">
        <source>
            <dbReference type="PubMed" id="32555463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="743" mass="84791" checksum="604FEBE9325890F4" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q980D4</accession>
    <name>Q980D4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sugar phosphate nucleotydyl transferase</fullName>
            <ecNumber evidence="11">2.7.7.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">SSO0381</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate + UTP + H(+) = UDP-N-acetyl-alpha-D-glucosamine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>alpha-D-glucosamine 1-phosphate + acetyl-CoA = N-acetyl-alpha-D-glucosamine 1-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="11"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90181">
        <property type="entry name" value="F90181"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980D4"/>
    <dbReference type="FunCoup" id="Q980D4">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0381"/>
    <dbReference type="PaxDb" id="273057-SSO0381"/>
    <dbReference type="EnsemblBacteria" id="AAK40709">
        <property type="protein sequence ID" value="AAK40709"/>
        <property type="gene ID" value="SSO0381"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0381"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.375"/>
    <dbReference type="eggNOG" id="arCOG00666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_1_2"/>
    <dbReference type="InParanoid" id="Q980D4"/>
    <dbReference type="PhylomeDB" id="Q980D4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050065">
        <property type="entry name" value="GlmU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584:SF8">
        <property type="entry name" value="N-ACETYLMURAMATE ALPHA-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584">
        <property type="entry name" value="NUCLEOTIDYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="9">
        <location>
            <begin position="5"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="10">
        <location>
            <begin position="255"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK40709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="363" mass="39481" checksum="E9679F76D152DC5B" modified="2001-10-01" version="1">MSEMKAIVLAGGKGEGLLPYTEKVQKEAITVLGKAIINYTIEGLKKAGVKEFEIIVNEKGNQIEEEVEKLDVSFETIVQKKQGIDGAVLEGIESIDDDVFVLAFGDIIAPAEFYKSLMDTYIMTGSQAVIPLIPISEGTETYGLVKIVDNKLKVVKESSTLALGGAYILPKPKDNFTSLLDYIDLLSSQARLNYFIWSESWVDIGYPEDLLFALETLLRRKESVISDKAEISKNAIIGKGVIIEDYAIIEDYAIIKGPAYIGKNAYVGSFSLVRDYSSIEEGAKIGAYCEIAHSLIEPFAEVGSKSYLTYSIVGKGAKIGASVITASYPAHSLSRPRFNKLGALISPEKVIKHGSVIEAGTKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2016-03-16" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q980D9</accession>
    <name>XERA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Tyrosine recombinase XerA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 6" type="primary">xerA</name>
        <name evidence="8" type="synonym">xerC/D</name>
        <name evidence="8" type="ordered locus">SSO0375</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="EMBO J." volume="30" first="145" last="153">
            <title>Replication termination and chromosome dimer resolution in the archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Duggin I.G."/>
                <person name="Dubarry N."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="21113132"/>
            <dbReference type="DOI" id="10.1038/emboj.2010.301"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>DNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. Probably involved in the resolution of chromosome dimers. Binds to the dif site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'phage' integrase family. XerA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99181">
        <property type="entry name" value="A99181"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990677.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980D9"/>
    <dbReference type="SMR" id="Q980D9"/>
    <dbReference type="FunCoup" id="Q980D9">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0375"/>
    <dbReference type="PaxDb" id="273057-SSO0375"/>
    <dbReference type="EnsemblBacteria" id="AAK40704">
        <property type="protein sequence ID" value="AAK40704"/>
        <property type="gene ID" value="SSO0375"/>
    </dbReference>
    <dbReference type="GeneID" id="1455514"/>
    <dbReference type="GeneID" id="44129348"/>
    <dbReference type="KEGG" id="sso:SSO0375"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.368"/>
    <dbReference type="eggNOG" id="arCOG01241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_9_2_2"/>
    <dbReference type="InParanoid" id="Q980D9"/>
    <dbReference type="PhylomeDB" id="Q980D9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009009">
        <property type="term" value="F:site-specific recombinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009037">
        <property type="term" value="F:tyrosine-based site-specific recombinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00796">
        <property type="entry name" value="INT_Rci_Hp1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02055">
        <property type="entry name" value="Recomb_XerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050090">
        <property type="entry name" value="Tyrosine_recombinase_XerCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033686">
        <property type="entry name" value="XerA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040815">
        <property type="entry name" value="recomb_XerA_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349:SF41">
        <property type="entry name" value="INTEGRASE_RECOMBINASE PROTEIN MJ0367-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349">
        <property type="entry name" value="PHAGE INTEGRASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13495">
        <property type="entry name" value="Phage_int_SAM_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0229">DNA integration</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000435796" description="Tyrosine recombinase XerA">
        <location>
            <begin position="1"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Core-binding (CB)" evidence="4">
        <location>
            <begin position="9"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="3">
        <location>
            <begin position="115"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="active site" evidence="1 2">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="active site" evidence="1 2">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="active site" evidence="1 2">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="active site" evidence="1 2">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <feature type="active site" evidence="1 2">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="1 2">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P0A8P8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="21113132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="6">
        <source>
            <dbReference type="PubMed" id="21113132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <evidence type="ECO:0000312" key="8">
        <source>
            <dbReference type="EMBL" id="AAK40704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK40704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="291" mass="33539" checksum="CDDB9519ED65B129" modified="2001-10-01" version="1">MKLDLGSPPESGDLYNAFINALIIAGAGNGTIKLYSTAVRDFLDFINKDPRKVTSEDLNRWISSLLNREGKVKGDEVEKKRAKSVTIRYYIIAVRRFLKWINVSVRPPIPKVRRKEVKALDEIQIQKVLNACKRTKDKLIIRLLLDTGLRANELLSVLVKDIDLENNMIRVRNTKNGEERIVFFTDETKLLLRKYIKGKKAEDKLFDLKYDTLYRKLKRLGKKVGIDLRPHILRHTFATLSLKRGINVITLQKLLGHKDIKTTQIYTHLVLDDLRNEYLKAMSSSSSKTPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q980E9</accession>
    <name>Q980E9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Prolidase (Xaa-Pro dipeptidase) (PepQ)</fullName>
            <ecNumber evidence="5">3.4.13.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pepQ</name>
        <name evidence="5" type="ordered locus">SSO0363</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.13.9" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90179">
        <property type="entry name" value="F90179"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990663.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980E9"/>
    <dbReference type="FunCoup" id="Q980E9">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0363"/>
    <dbReference type="PaxDb" id="273057-SSO0363"/>
    <dbReference type="EnsemblBacteria" id="AAK40693">
        <property type="protein sequence ID" value="AAK40693"/>
        <property type="gene ID" value="SSO0363"/>
    </dbReference>
    <dbReference type="GeneID" id="1455503"/>
    <dbReference type="KEGG" id="sso:SSO0363"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.357"/>
    <dbReference type="eggNOG" id="arCOG01000">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_4_0_2"/>
    <dbReference type="InParanoid" id="Q980E9"/>
    <dbReference type="PhylomeDB" id="Q980E9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102009">
        <property type="term" value="F:proline dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01092">
        <property type="entry name" value="APP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050659">
        <property type="entry name" value="Peptidase_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112:SF9">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="3">
        <location>
            <begin position="137"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="352" mass="39538" checksum="CF45B4004A9639FB" modified="2001-10-01" version="1">MNRLRKLKAILEDKKDSCAIIFGSPNLFYFLEYSGAGALIYCDSRFTLLVPALEMYRAENVKDVDIAIYYPSKILENVIEGNIFRAIEKIVNKSNIMLDVSWVEASTYKALSEKYKVIDFSNEIVRLREVKDNDEIERIRKAGEITAVAMKIGMEKLSEGTSNEKQVAGIIDMTMRSMGAEDYAFPSIVAFGENSAYPHHIPTDRVLGNNDIVLFDIGAKYNGYCFDSTRTFVFKNSEAKKVYEVVLEAQMEAIDAVRDGVMASEVDITARRVIEKAGYGKYFIHSTGHGVGVEIHESPAVSMNSKQILKENMIITVEPGIYLKGRFGIRIEDTLIVTKGKPIVLETAYKLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-03" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q980G0</accession>
    <name>IF6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 6</fullName>
            <shortName evidence="1">aIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif6</name>
        <name type="ordered locus">SSO0351</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2009" name="Nucleic Acids Res." volume="37" first="256" last="267">
            <title>Function and ribosomal localization of aIF6, a translational regulator shared by archaea and eukarya.</title>
            <authorList>
                <person name="Benelli D."/>
                <person name="Marzi S."/>
                <person name="Mancone C."/>
                <person name="Alonzi T."/>
                <person name="la Teana A."/>
                <person name="Londei P."/>
            </authorList>
            <dbReference type="PubMed" id="19036786"/>
            <dbReference type="DOI" id="10.1093/nar/gkn959"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH L14 (RPL14P)</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>POSSIBLE POST-TRANSLATIONAL MODIFICATION</scope>
        <scope>INDUCTION</scope>
        <scope>RRNA-BINDING</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Binds to the 50S ribosomal subunit and prevents its association with the 30S ribosomal subunit to form the 70S initiation complex. Inhibits translation of both leadered and leaderless mRNAs, maybe by binding to the 50S ribosome subunit, preventing it from binding to the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Associates with the 50S ribosomal subunit, specifically with protein L14. Binds to 23S rRNA, possibly between where the 30S and 50S subunits associate to initiate translation.</text>
    </comment>
    <comment type="induction">
        <text evidence="2">Constitutively expressed, further induced by cold (60) or heat (90 degrees Celsius) shock (at protein level).</text>
    </comment>
    <comment type="PTM">
        <text>Modified in an unknown fashion (not phosphorylation) following release from 50S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90178">
        <property type="entry name" value="B90178"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990645.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980G0"/>
    <dbReference type="SMR" id="Q980G0"/>
    <dbReference type="FunCoup" id="Q980G0">
        <property type="interactions" value="230"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0351"/>
    <dbReference type="PaxDb" id="273057-SSO0351"/>
    <dbReference type="EnsemblBacteria" id="AAK40681">
        <property type="protein sequence ID" value="AAK40681"/>
        <property type="gene ID" value="SSO0351"/>
    </dbReference>
    <dbReference type="GeneID" id="1455491"/>
    <dbReference type="KEGG" id="sso:SSO0351"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.342"/>
    <dbReference type="eggNOG" id="arCOG04176">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071894_1_0_2"/>
    <dbReference type="InParanoid" id="Q980G0"/>
    <dbReference type="PhylomeDB" id="Q980G0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.75.10.10:FF:000011">
        <property type="entry name" value="Translation initiation factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003126">
        <property type="entry name" value="PRK04046.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006413">
        <property type="entry name" value="IF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_0000153759" description="Translation initiation factor 6">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="19036786"/>
        </source>
    </evidence>
    <sequence length="223" mass="24373" checksum="764B8D4CDA444C68" modified="2001-10-01" version="1">MNLQRLSVFGTDNIGVYIYTNNKYTIIPRGLDSETKENIAQVLGTELLEAEISRSFLLGIFISGNDNGILLPKSTIDDEFRFLKENLRDCRVEILNSKVTALGNTILANNKAALIYPEFNDIEEKIIKETLGVEDIKRGKIAQMITVGSVGVITNKGGLVHVDTSEKELKELEKLFGVKIDIGTVNFGSVFIKSGLVANDKGTLVGASTTGPEILRIQKALGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q980G5</accession>
    <name>Q980G5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0334</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90176">
        <property type="entry name" value="D90176"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980G5"/>
    <dbReference type="STRING" id="273057.SSO0334"/>
    <dbReference type="PaxDb" id="273057-SSO0334"/>
    <dbReference type="EnsemblBacteria" id="AAK40667">
        <property type="protein sequence ID" value="AAK40667"/>
        <property type="gene ID" value="SSO0334"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0334"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.327"/>
    <dbReference type="eggNOG" id="arCOG06041">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051793_0_0_2"/>
    <dbReference type="InParanoid" id="Q980G5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="433" mass="46789" checksum="378F252263553382" modified="2001-10-01" version="1">MYLSPSNATAEVIANDTVFFGFVNQGQPNFPMIYDIHVAITAYFVYNITTASWQITKEIWNSTEVNALSDTVYYNLNEPIMKVSGIDTVTINASSGQGYTLQVGNVMVIVKPDTYAELANGTMLSVYNFSLVTFSLEAIYSPPTAPNLTPLYAFAFAVNNQITPAISLVTMSNGKVIAQPPITVVWAPNTWTSWTWFGGTFNGTAYIGGSYKFVDHWLYGNGVMVNVQFFKPVIWIFESSETPVAQTPKPVQVSVSSVYGLTPVNVYSYEINGTQGGVAVAGNMIVTIQPNTKIVTPSGNLSVFNFSIVFYNPANAGNAPNGQVPILAFAYAINGNVTFSYSSVNSITGLANPFITVILTPSQNVSMWTWGAKGYIFKDPIIVGNGIVINLTFFKPVPWILTAPEISSSSSTPMTSSSSTSTTTTSTSSPYWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q980H0</accession>
    <name>Q980H0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADH dehydrogenase subunit J (NuoJ)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">NuoJ</name>
        <name evidence="2" type="ordered locus">SSO0327</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90175">
        <property type="entry name" value="G90175"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990620.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980H0"/>
    <dbReference type="FunCoup" id="Q980H0">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0327"/>
    <dbReference type="PaxDb" id="273057-SSO0327"/>
    <dbReference type="EnsemblBacteria" id="AAK40662">
        <property type="protein sequence ID" value="AAK40662"/>
        <property type="gene ID" value="SSO0327"/>
    </dbReference>
    <dbReference type="GeneID" id="1455472"/>
    <dbReference type="KEGG" id="sso:SSO0327"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.321"/>
    <dbReference type="eggNOG" id="arCOG04654">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582963_0_0_2"/>
    <dbReference type="InParanoid" id="Q980H0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1200">
        <property type="entry name" value="NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001457">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042106">
        <property type="entry name" value="Nuo/plastoQ_OxRdtase_6_NuoJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33269">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33269:SF17">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00499">
        <property type="entry name" value="Oxidored_q3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="167" mass="18292" checksum="5573586E46050876" modified="2001-10-01" version="1">MYPQTSEIIQYILFGFFGVMTIAFSIYIVRAKNVFYGAVSLAFLGLSIAALIALEAPSTYGIYSVFHILLYVGATVTFLAISLVMFKDLEVKFGRGALGILAGGAVTLLFLIIVFISIPQVSSAEVQPINFQVLANDLIENYWFPLIILVIALLTTLIEAISLARRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-25" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q980L7</accession>
    <name>ATGT_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-guanine(15) transglycosylase</fullName>
            <ecNumber evidence="1">2.4.2.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7-cyano-7-deazaguanine tRNA-ribosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Archaeal tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tgtA</name>
        <name type="ordered locus">SSO0274</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Exchanges the guanine residue with 7-cyano-7-deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D-loop) of archaeal tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(15) in tRNA + 7-cyano-7-carbaguanine = 7-cyano-7-carbaguanosine(15) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:43164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10371"/>
            <dbReference type="Rhea" id="RHEA-COMP:10372"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:82850"/>
            <dbReference type="EC" id="2.4.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; archaeosine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeosine tRNA-ribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.48" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90169">
        <property type="entry name" value="F90169"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990551.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980L7"/>
    <dbReference type="SMR" id="Q980L7"/>
    <dbReference type="FunCoup" id="Q980L7">
        <property type="interactions" value="178"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0274"/>
    <dbReference type="PaxDb" id="273057-SSO0274"/>
    <dbReference type="EnsemblBacteria" id="AAK40613">
        <property type="protein sequence ID" value="AAK40613"/>
        <property type="gene ID" value="SSO0274"/>
    </dbReference>
    <dbReference type="GeneID" id="1455423"/>
    <dbReference type="GeneID" id="44129246"/>
    <dbReference type="KEGG" id="sso:SSO0274"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.269"/>
    <dbReference type="eggNOG" id="arCOG00989">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030083_0_0_2"/>
    <dbReference type="InParanoid" id="Q980L7"/>
    <dbReference type="PhylomeDB" id="Q980L7"/>
    <dbReference type="UniPathway" id="UPA00393"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002099">
        <property type="term" value="P:tRNA wobble guanine modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10630">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01634">
        <property type="entry name" value="TgtA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050076">
        <property type="entry name" value="ArchSynthase1/Queuine_TRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004804">
        <property type="entry name" value="TgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00432">
        <property type="entry name" value="arcsn_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000247885" description="tRNA-guanine(15) transglycosylase">
        <location>
            <begin position="1"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01634"/>
        </source>
    </evidence>
    <sequence length="503" mass="58318" checksum="B933A240FB554BCE" modified="2001-10-01" version="1">MTVFEVKYEDLAGRIGTLRTRSGTLETPAFFPVINVLKKDEISIDEIRNIGFKNFITNSYILYKNNYIKDDIHKELRSEEMIIMTDSGAYQILEYGEIGITNLQIVNYQLKIKPDIGVILDLPTGNINDYDNAKKTVYETLKRAEEASEIIVKNQDNNIIWVYPIQGGRYLDLVKTSAEGLSKFEHIYNMAALGSPTVLLEKYMYDTVIDMIYTAKSNIKRGIPFHLFGGGLPHIIPFAVALGVDSFDSASYIIYARDNRYITRTRVYKLEDLEYFPCSCPICSKYTPKDLLEMNEKERTKALAIHNLYTILEEFKATKQAIKEGRLFEYLQEKAYSHPAVYSAFKRLMKYKDYLEKFDPRIRGDPKGLFLFDGNSLHRPEIIRHSRFLERYIQKKDKISIYCYDKAISDTAYDFKEKIREKIADRNESDVFIAVPFFGLIPLEISDSYPLSQFEIPNEIDEDVIDDMKTKIISFLRRNNYQKVELINCEKLGLHIDSISTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q980R6</accession>
    <name>Q980R6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1464 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0214</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90162">
        <property type="entry name" value="F90162"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980R6"/>
    <dbReference type="STRING" id="273057.SSO0214"/>
    <dbReference type="PaxDb" id="273057-SSO0214"/>
    <dbReference type="EnsemblBacteria" id="AAK40557">
        <property type="protein sequence ID" value="AAK40557"/>
        <property type="gene ID" value="SSO0214"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0214"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.212"/>
    <dbReference type="eggNOG" id="arCOG04329">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075234_0_0_2"/>
    <dbReference type="InParanoid" id="Q980R6"/>
    <dbReference type="PhylomeDB" id="Q980R6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009927">
        <property type="entry name" value="DUF1464"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07318">
        <property type="entry name" value="DUF1464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009433">
        <property type="entry name" value="DUF1464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="34339" checksum="2C96C68F795E0279" modified="2001-10-01" version="1">MARQPLMIYVGIDPGSETYAFAFVDELGNLIKYFEICTDLVEKSSIFLAKLIADYRPMAVALPSGHGLPFYNIRNINNREIFILTLKDPLSHGPLRDFLLASKQYLSFYNSFTIPSVIELNSLPNDRKVNVIDKGTADKVASTFFYRVYLKLDNFILVEMGRRFTAIVIVINGKIVDGYGGTYLSGLDGEIAYLLHKYSRIDKSTVYNVGRNLELTRIIVEWYSDKYKLPIIVSGYNKNYLEIGKKYDFKFKEAAVGAAFIANAYSGGLLRHYISMLDSSGTPISFVRLKEWDEIISWIETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q980X2</accession>
    <name>Q980X2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90155">
        <property type="entry name" value="E90155"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980X2"/>
    <dbReference type="STRING" id="273057.SSO0154"/>
    <dbReference type="PaxDb" id="273057-SSO0154"/>
    <dbReference type="EnsemblBacteria" id="AAK40500">
        <property type="protein sequence ID" value="AAK40500"/>
        <property type="gene ID" value="SSO0154"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0154"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.151"/>
    <dbReference type="eggNOG" id="arCOG05463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1821061_0_0_2"/>
    <dbReference type="InParanoid" id="Q980X2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="142" mass="16322" checksum="B1231F4931B6CB5D" modified="2001-10-01" version="1">MMSVNTDELKKFLESKITSLRKELEFYEFLLSIIESGYIPTIKGNKGSIEYIKNKKGEILAEIYFTPPVAKFVIKNKITLSKSYFSALSKILESEKELNKIDYTIEMDKNDIREIVLKNVNSDIIYNKIKTGIQAILERATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Y0</accession>
    <name>Q980Y0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Primase (Amino-end)</fullName>
            <ecNumber evidence="3">2.7.7.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0142</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.-" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90154">
        <property type="entry name" value="E90154"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922900.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Y0"/>
    <dbReference type="PaxDb" id="273057-SSO0142"/>
    <dbReference type="EnsemblBacteria" id="AAK40492">
        <property type="protein sequence ID" value="AAK40492"/>
        <property type="gene ID" value="SSO0142"/>
    </dbReference>
    <dbReference type="GeneID" id="1453319"/>
    <dbReference type="KEGG" id="sso:SSO0142"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.134"/>
    <dbReference type="eggNOG" id="arCOG06915">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_679018_0_0_2"/>
    <dbReference type="InParanoid" id="Q980Y0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04859">
        <property type="entry name" value="Prim_Pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2250.10">
        <property type="entry name" value="Bifunctional DNA primase/polymerase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.160">
        <property type="entry name" value="Bifunctional DNA primase/polymerase, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.130">
        <property type="entry name" value="Zinc stem-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015330">
        <property type="entry name" value="DNA_primase/pol_bifunc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024966">
        <property type="entry name" value="Primase_helical_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09250">
        <property type="entry name" value="Prim-Pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13010">
        <property type="entry name" value="pRN1_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00943">
        <property type="entry name" value="Prim-Pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA primase/polymerase bifunctional N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="219"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="405" mass="47312" checksum="D32B2B1771F5121A" modified="2001-10-01" version="1">MDKIEWAKWFIDTLGFAVIPIDRDTKKPTIPNWQQYSTRALTDEEKEKFPKMIEEGYNYGIPGQMLDKNRALVILDFEDKELLKAWIGESELNKLCQNTLCVTTPHNGLHIYAITEEFPEYKFNPAFLRENKNVMDLQSLKSYVVAPGSCINHKFCDSAKCPWKGQDYVTCYYADITPERAKINEIHLKGFLKYLVEKGKKLGITPASGLLEWLGGNKKSDTEEDFEKLKEEMRKYDRYRGKTLDAIRDELCKAIKKKIEHAEEKAKETLNIAKGVLCDKKTYADIGIDRSRGDWHLLNFLLSHGATDLDVVMQLLPEDSKVFEPKWDKYTFHTVAKIWERVKPWLTEFRNGDKKTELKLRGALIISNLLRKKYVIRTFYQDMGQWCFTKLFHLKDIILVLSKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q981C3</accession>
    <name>Q981C3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90141">
        <property type="entry name" value="F90141"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989622.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981C3"/>
    <dbReference type="PaxDb" id="273057-SSO0022"/>
    <dbReference type="EnsemblBacteria" id="AAK40389">
        <property type="protein sequence ID" value="AAK40389"/>
        <property type="gene ID" value="SSO0022"/>
    </dbReference>
    <dbReference type="GeneID" id="1455280"/>
    <dbReference type="KEGG" id="sso:SSO0022"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.25"/>
    <dbReference type="eggNOG" id="arCOG05907">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1773287_0_0_2"/>
    <dbReference type="InParanoid" id="Q981C3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="146" mass="17270" checksum="7F96CB44B8E2E7D6" modified="2001-10-01" version="1">MISWLLGSQPSPWSFLEDLFQDYRNVAIYVDNNNIVQIVKVSDIDEFYTPFSVLIHAKYFKNYSPYYIKLEKMVAFPTTNEKVADSLLIKEGWRGIKYYYGDEFLGAWILYDCIKCRDKQRSHLEISKLSISEDETIKAHLRIYNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q981C4</accession>
    <name>Q981C4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase, soxB-like, putative (SoxB-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">soxB-like</name>
        <name evidence="2" type="ordered locus">SSO0021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90141">
        <property type="entry name" value="E90141"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981C4"/>
    <dbReference type="STRING" id="273057.SSO0021"/>
    <dbReference type="PaxDb" id="273057-SSO0021"/>
    <dbReference type="EnsemblBacteria" id="AAK40388">
        <property type="protein sequence ID" value="AAK40388"/>
        <property type="gene ID" value="SSO0021"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0021"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.24"/>
    <dbReference type="eggNOG" id="arCOG00755">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1472152_0_0_2"/>
    <dbReference type="InParanoid" id="Q981C4"/>
    <dbReference type="PhylomeDB" id="Q981C4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="183" mass="20558" checksum="D0F4C6C7733D2F90" modified="2001-10-01" version="1">MIILSAGAWSKYLINVKLPVKTYYCWASLFLSKKKEVGSNIIYDYVNNFYSRPVIGLGFPLAIMGDGKAIETTPLQQNICIEDKNEVSARISRRLGEVKELYTSGSFCEATPDMRPAYGKIAENVYFIGGFNGYGAEVGPGLAHVLVEEILSKKEDTYFTEYRLERLNGYDGNFEIGQEPHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q981D3</accession>
    <name>Q981D3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO5027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90140">
        <property type="entry name" value="D90140"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989637.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981D3"/>
    <dbReference type="PaxDb" id="273057-SSO5027"/>
    <dbReference type="EnsemblBacteria" id="AAK40379">
        <property type="protein sequence ID" value="AAK40379"/>
        <property type="gene ID" value="SSO5027"/>
    </dbReference>
    <dbReference type="GeneID" id="1455269"/>
    <dbReference type="KEGG" id="sso:SSO5027"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.13"/>
    <dbReference type="eggNOG" id="arCOG07275">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2949461_0_0_2"/>
    <dbReference type="InParanoid" id="Q981D3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045910">
        <property type="entry name" value="Sul7s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="59" mass="7227" checksum="8488B3A72AC00786" modified="2001-10-01" version="1">MEDVKQSVEKIIKDREWITFNDLLKYIPYPAPEVYNALSQLIKENKVGRRGRYFYYIKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-09-05" modified="2026-01-28" version="144" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UWX2</accession>
    <name>SYP_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proline--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Prolyl-tRNA synthetase</fullName>
            <shortName evidence="1">ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">proS</name>
        <name type="ordered locus">SSO0569</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90203">
        <property type="entry name" value="E90203"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991082.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UWX2"/>
    <dbReference type="SMR" id="Q9UWX2"/>
    <dbReference type="FunCoup" id="Q9UWX2">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0569"/>
    <dbReference type="PaxDb" id="273057-SSO0569"/>
    <dbReference type="EnsemblBacteria" id="AAK40884">
        <property type="protein sequence ID" value="AAK40884"/>
        <property type="gene ID" value="SSO0569"/>
    </dbReference>
    <dbReference type="GeneID" id="1454851"/>
    <dbReference type="GeneID" id="44129573"/>
    <dbReference type="KEGG" id="sso:SSO0569"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.577"/>
    <dbReference type="eggNOG" id="arCOG00402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_4_2_2"/>
    <dbReference type="InParanoid" id="Q9UWX2"/>
    <dbReference type="PhylomeDB" id="Q9UWX2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00862">
        <property type="entry name" value="ProRS_anticodon_zinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00778">
        <property type="entry name" value="ProRS_core_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000037">
        <property type="entry name" value="Proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01571">
        <property type="entry name" value="Pro_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016061">
        <property type="entry name" value="Pro-tRNA_ligase_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033721">
        <property type="entry name" value="ProRS_core_arch_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00408">
        <property type="entry name" value="proS_fam_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382:SF2">
        <property type="entry name" value="BIFUNCTIONAL GLUTAMATE_PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PROLYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09180">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SMART" id="SM00946">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000249174" description="Proline--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01571"/>
        </source>
    </evidence>
    <sequence length="481" mass="55852" checksum="1EE34AFE910F72CF" modified="2000-05-01" version="1">MQITREKWSKNFSEWFDWVLREGEFYDYGRYPIKGMGVWMPYGFKLRQNIIGIIRNFLDSTGHEEVLFPLLIPEDLLRRESTHIKGFEEEVFWVTKGGSTDLDVKLALRPTSEVAITTMENLWLKSYKQLPKKYYQIVSVFRYETKATRPMIRLREITTFKEAHTVHETYDDAQKQVEEAIEIYKKIFDTLTIPYVLSERPEWDRFAGALHTYAFDTIMPDGRAMQIGTVHHLGQNFSKALDFKIQKKDGSLDYPHQTSYGISDRAVASVIAIHGDDHGPVLPPSVAPIKVVVIPIPAKNEEETQQVMKYSIEICEMFNKNNITCLTDQDTEKTPGEKFYIWEIKGVPIRLEIGPRELISGTVFIKRRDNFKSYTVRKEEAINKIKELLNEIQEDLRRKAWEDLKSRIEYSNDIEKAKKILGNNSGIVEVPWCGSNECGLKIEELTNARVLGYPIEKREVNDKCVICKMNAKTVLRIAKTY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-11-16" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX09</accession>
    <name>PYRE_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="1">OPRT</shortName>
            <shortName evidence="1">OPRTase</shortName>
            <ecNumber evidence="1">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrE</name>
        <name type="ordered locus">SSO0615</name>
        <name type="ORF">C08_035</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90208">
        <property type="entry name" value="G90208"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991146.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX09"/>
    <dbReference type="SMR" id="Q9UX09"/>
    <dbReference type="FunCoup" id="Q9UX09">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0615"/>
    <dbReference type="PaxDb" id="273057-SSO0615"/>
    <dbReference type="EnsemblBacteria" id="AAK40926">
        <property type="protein sequence ID" value="AAK40926"/>
        <property type="gene ID" value="SSO0615"/>
    </dbReference>
    <dbReference type="GeneID" id="1454893"/>
    <dbReference type="GeneID" id="44129617"/>
    <dbReference type="KEGG" id="sso:SSO0615"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.623"/>
    <dbReference type="eggNOG" id="arCOG00029">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074878_2_0_2"/>
    <dbReference type="InParanoid" id="Q9UX09"/>
    <dbReference type="PhylomeDB" id="Q9UX09"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006222">
        <property type="term" value="P:UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000058">
        <property type="entry name" value="Orotidine-5-phosphate decarboxylase/orotate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278:SF9">
        <property type="entry name" value="URIDINE 5'-MONOPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000110790" description="Orotate phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="111"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <sequence length="195" mass="21614" checksum="E4CF07445B7FEBF4" modified="2000-05-01" version="1">MNFAEVLLERKLLLIGSFVLTSGKVSPYYLDLRPLPNYPEFYDIVNQAIKKAKDIPHDIIVGIATGGVPLSAFIACNLKEPMGYIRIEKKGHGTNRTLELDVKGKRVLLVDDVATTGVSIEKATLEILNGGGKVSDALVIIDRQEGASQRLEKLGVKLHSLFKISEILDELLKSDKLKDNEKKSILDYLVKNVEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXG3</accession>
    <name>CDPAS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CDP-archaeol synthase</fullName>
            <ecNumber evidence="1">2.7.7.67</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">carS</name>
        <name type="ordered locus">SSO0776</name>
        <name type="ORF">C40_012</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol (CDP-archaeol) from 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate (DGGGP) and CTP. This reaction is the third ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + CTP + H(+) = CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:25690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:58838"/>
            <dbReference type="EC" id="2.7.7.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDP-archaeol synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.67" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99227">
        <property type="entry name" value="B99227"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923068.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXG3"/>
    <dbReference type="SMR" id="Q9UXG3"/>
    <dbReference type="STRING" id="273057.SSO0776"/>
    <dbReference type="PaxDb" id="273057-SSO0776"/>
    <dbReference type="EnsemblBacteria" id="AAK41073">
        <property type="protein sequence ID" value="AAK41073"/>
        <property type="gene ID" value="SSO0776"/>
    </dbReference>
    <dbReference type="GeneID" id="15297723"/>
    <dbReference type="KEGG" id="sso:SSO0776"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.777"/>
    <dbReference type="eggNOG" id="arCOG04106">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105710_0_0_2"/>
    <dbReference type="InParanoid" id="Q9UXG3"/>
    <dbReference type="PhylomeDB" id="Q9UXG3"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043338">
        <property type="term" value="F:CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01117">
        <property type="entry name" value="CDP_archaeol_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032690">
        <property type="entry name" value="CarS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002726">
        <property type="entry name" value="CarS_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003114">
        <property type="entry name" value="PRK04032.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39650">
        <property type="entry name" value="CDP-ARCHAEOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39650:SF1">
        <property type="entry name" value="CDP-ARCHAEOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01864">
        <property type="entry name" value="CarS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000094180" description="CDP-archaeol synthase">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01117"/>
        </source>
    </evidence>
    <sequence length="166" mass="18484" checksum="D8092159D0958F4F" modified="2000-05-01" version="1">MSIAYDLLLSILIYLPAFVANGSGPFIKRGTPIDFGKNFVDGRRLFGDGKTFEGLIVALTFGTTVGVIISKFFTAEWTLISFLESLFAMIGDMIGAFIKRRLGIPRGGRVLGLDQLDFVLGASLILVLMRVNITWYQFLFICGLAFFLHQGTNYVAYLLKIKNVPW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="149" xmlns="https://uniprot.org/uniprot">
    <accession>O33768</accession>
    <accession>Q9UWZ3</accession>
    <name>SYL2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Leucine--tRNA ligase 2</fullName>
            <ecNumber evidence="1">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Leucyl-tRNA synthetase 2</fullName>
            <shortName evidence="1">LeuRS 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuS2</name>
        <name type="synonym">leuS</name>
        <name type="ordered locus">SSO0589</name>
        <name type="ORF">C21_009</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1997" name="J. Bacteriol." volume="179" first="4429" last="4432">
            <title>Evolutionary analysis of the hisCGABdFDEHI gene cluster from the archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Sensen C.W."/>
                <person name="Doolittle W.F."/>
                <person name="Brown J.R."/>
            </authorList>
            <dbReference type="PubMed" id="9209067"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 762-944</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="U82227">
        <property type="protein sequence ID" value="AAB63016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90205">
        <property type="entry name" value="G90205"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991108.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O33768"/>
    <dbReference type="SMR" id="O33768"/>
    <dbReference type="FunCoup" id="O33768">
        <property type="interactions" value="285"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0589"/>
    <dbReference type="PaxDb" id="273057-SSO0589"/>
    <dbReference type="EnsemblBacteria" id="AAK40902">
        <property type="protein sequence ID" value="AAK40902"/>
        <property type="gene ID" value="SSO0589"/>
    </dbReference>
    <dbReference type="GeneID" id="1454869"/>
    <dbReference type="GeneID" id="44129592"/>
    <dbReference type="KEGG" id="sso:SSO0589"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.598"/>
    <dbReference type="eggNOG" id="arCOG00809">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004174_0_0_2"/>
    <dbReference type="InParanoid" id="O33768"/>
    <dbReference type="PhylomeDB" id="O33768"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07959">
        <property type="entry name" value="Anticodon_Ia_Leu_AEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00812">
        <property type="entry name" value="LeuRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.20">
        <property type="entry name" value="Class I aminoacyl-tRNA synthetases (RS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.720">
        <property type="entry name" value="leucyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_A">
        <property type="entry name" value="Leu_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020791">
        <property type="entry name" value="Leu-tRNA-lgase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008957">
        <property type="entry name" value="PRK12300.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152145" description="Leucine--tRNA ligase 2">
        <location>
            <begin position="1"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="623"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="626"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <sequence length="944" mass="109104" checksum="9ACAFCFBF04160DC" modified="2001-06-20" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1990-04-01" modified="2026-01-28" version="157" xmlns="https://uniprot.org/uniprot">
    <accession>P14909</accession>
    <name>AAT_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Aspartate aminotransferase</fullName>
            <shortName>AspAT</shortName>
            <ecNumber>2.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Transaminase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">aspC</name>
        <name type="ordered locus">SSO0897</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1989" name="Eur. J. Biochem." volume="186" first="375" last="381">
            <title>Cloning and sequencing of the gene coding for aspartate aminotransferase from the thermoacidophilic archaebacterium Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Cubellis M.V."/>
                <person name="Rozzo C."/>
                <person name="Nitti G."/>
                <person name="Arnone M.I."/>
                <person name="Marino G."/>
                <person name="Sannia G."/>
            </authorList>
            <dbReference type="PubMed" id="2513189"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1989.tb15219.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 2-39 AND 297-327</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1994" name="Eur. J. Biochem." volume="222" first="761" last="767">
            <title>Post-translational modifications in aspartate aminotransferase from Sulfolobus solfataricus. Detection of N-epsilon-methyllysines by mass spectrometry.</title>
            <authorList>
                <person name="Zappacosta F."/>
                <person name="Sannia G."/>
                <person name="Savoy L.-A."/>
                <person name="Marino G."/>
                <person name="Pucci P."/>
            </authorList>
            <dbReference type="PubMed" id="8026489"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1994.tb18922.x"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-402</scope>
        <scope>METHYLATION AT LYS-203 AND LYS-385</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="1988" name="J. Biol. Chem." volume="263" first="12305" last="12309">
            <title>Purification and characterization of aspartate aminotransferase from the thermoacidophilic archaebacterium Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Marino G."/>
                <person name="Nitti G."/>
                <person name="Arnone M.I."/>
                <person name="Sannia G."/>
                <person name="Gambacorta A."/>
                <person name="de Rosa M."/>
            </authorList>
            <dbReference type="PubMed" id="3137225"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-23</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="1991" name="Biochim. Biophys. Acta" volume="1080" first="198" last="204">
            <title>The active site of Sulfolobus solfataricus aspartate aminotransferase.</title>
            <authorList>
                <person name="Birolo L."/>
                <person name="Arnone M.I."/>
                <person name="Cubellis M.V."/>
                <person name="Andreotti G."/>
                <person name="Nitti G."/>
                <person name="Marino G."/>
                <person name="Sannia G."/>
            </authorList>
            <dbReference type="PubMed" id="1954227"/>
            <dbReference type="DOI" id="10.1016/0167-4838(91)90002-H"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 240-251</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text>Optimum temperature is above 95 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text>Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1"/>
    <dbReference type="EMBL" id="X16505">
        <property type="protein sequence ID" value="CAA34514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S07088">
        <property type="entry name" value="S07088"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992318.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P14909"/>
    <dbReference type="SMR" id="P14909"/>
    <dbReference type="FunCoup" id="P14909">
        <property type="interactions" value="239"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0897"/>
    <dbReference type="iPTMnet" id="P14909"/>
    <dbReference type="PaxDb" id="273057-SSO0897"/>
    <dbReference type="EnsemblBacteria" id="AAK41178">
        <property type="protein sequence ID" value="AAK41178"/>
        <property type="gene ID" value="SSO0897"/>
    </dbReference>
    <dbReference type="GeneID" id="1455145"/>
    <dbReference type="KEGG" id="sso:SSO0897"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.899"/>
    <dbReference type="eggNOG" id="arCOG01130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_2"/>
    <dbReference type="InParanoid" id="P14909"/>
    <dbReference type="PhylomeDB" id="P14909"/>
    <dbReference type="BioCyc" id="MetaCyc:MONOMER-13008"/>
    <dbReference type="BRENDA" id="2.6.1.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF1">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="initiator methionine" description="Removed" evidence="2 3 4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000123864" description="Aspartate aminotransferase">
        <location>
            <begin position="2"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-methyllysine; partial" evidence="4">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine; partial" evidence="4">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="2513189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="3137225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="8026489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="402" mass="45732" checksum="99A87B354990AF0E" modified="2007-01-23" version="2">MVSLLDFNGNMSQVTGETTLLYKEIARNVEKTKKIKIIDFGIGQPDLPTFKRIRDAAKEALDQGFTFYTSAFGIDELREKIAQYLNTRYGTDVKKEEVIVTPGAKPALFLVFILYINPSDEVILPDPSFYSYAEVVKLLGGKPIYANLKWSREEGFSIDVDDLQSKISKRTKMIVFNNPHNPTGTLFSPNDVKKIVDISRDNKIILLSDEIYDNFVYEGKMRSTLEDSDWRDFLIYVNGFSKTFSMTGWRLGYIVAKREIIQKMGILAANVYTAPTSFVQKAAVKAFDTFDEVNQMVSLFKKRRDVMYDELTKVKGVEVSKPNGAFYMFPNVSKILKTSGFDVKSLAIKLIEEKGVVTIPGEVFPLNIGKEFLRLSFAVNEEVIKEGIQKIREFAEQMMNSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1991-08-01" modified="2026-01-28" version="173" xmlns="https://uniprot.org/uniprot">
    <accession>P22498</accession>
    <accession>Q9V2Z5</accession>
    <name>BGAL_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Beta-galactosidase</fullName>
            <shortName>Lactase</shortName>
            <ecNumber>3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">lacS</name>
        <name type="ordered locus">SSO3019</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1990" name="Gene" volume="94" first="89" last="94">
            <title>Isolation and sequencing of a new beta-galactosidase-encoding archaebacterial gene.</title>
            <authorList>
                <person name="Cubellis M.V."/>
                <person name="Rozzo C."/>
                <person name="Montecucchi P."/>
                <person name="Rossi M."/>
            </authorList>
            <dbReference type="PubMed" id="2121622"/>
            <dbReference type="DOI" id="10.1016/0378-1119(90)90472-4"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 1-33</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="J. Bacteriol." volume="181" first="3920" last="3927">
            <title>Coordinate transcriptional control in the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Haseltine C."/>
                <person name="Montalvo-Rodriguez R."/>
                <person name="Bini E."/>
                <person name="Carl A."/>
                <person name="Blum P."/>
            </authorList>
            <dbReference type="PubMed" id="10383958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>98/2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2004" name="J. Biol. Chem." volume="279" first="10185" last="10194">
            <title>Thermal stability and aggregation of Sulfolobus solfataricus beta-glycosidase are dependent upon the N-epsilon-methylation of specific lysyl residues: critical role of in vivo post-translational modifications.</title>
            <authorList>
                <person name="Febbraio F."/>
                <person name="Andolfo A."/>
                <person name="Tanfani F."/>
                <person name="Briante R."/>
                <person name="Gentile F."/>
                <person name="Formisano S."/>
                <person name="Vaccaro C."/>
                <person name="Scire A."/>
                <person name="Bertoli E."/>
                <person name="Pucci P."/>
                <person name="Nucci R."/>
            </authorList>
            <dbReference type="PubMed" id="14660666"/>
            <dbReference type="DOI" id="10.1074/jbc.M308520200"/>
        </citation>
        <scope>METHYLATION AT LYS-116; LYS-135; LYS-273; LYS-311 AND LYS-332</scope>
        <scope>MASS SPECTROMETRY</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="1997" name="J. Mol. Biol." volume="271" first="789" last="802">
            <title>Crystal structure of the beta-glycosidase from the hyperthermophilic archeon Sulfolobus solfataricus: resilience as a key factor in thermostability.</title>
            <authorList>
                <person name="Aguilar C.F."/>
                <person name="Sanderson I."/>
                <person name="Moracci M."/>
                <person name="Ciaramella M."/>
                <person name="Nucci R."/>
                <person name="Rossi M."/>
                <person name="Pearl L.H."/>
            </authorList>
            <dbReference type="PubMed" id="9299327"/>
            <dbReference type="DOI" id="10.1006/jmbi.1997.1215"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS)</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text>Homotetramer.</text>
    </comment>
    <comment type="mass spectrometry" mass="56754.8" error="4.9" method="Electrospray" evidence="3"/>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The DNA coding for this protein is not found in the complete genome of strain 98/2. Sequence in PubMed:10383958 could have originated from another strain.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23"/>
    <dbReference type="EMBL" id="M34696">
        <property type="protein sequence ID" value="AAA72843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF133096">
        <property type="protein sequence ID" value="AAD21094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90483">
        <property type="entry name" value="B90483"/>
    </dbReference>
    <dbReference type="PIR" id="JQ0767">
        <property type="entry name" value="JQ0767"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992676.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1GOW">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A/B=1-489"/>
    </dbReference>
    <dbReference type="PDB" id="1UWI">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.55 A"/>
        <property type="chains" value="A/B/C/D=1-489"/>
    </dbReference>
    <dbReference type="PDB" id="1UWQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.02 A"/>
        <property type="chains" value="A/B=1-489"/>
    </dbReference>
    <dbReference type="PDB" id="1UWR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.14 A"/>
        <property type="chains" value="A/B=1-489"/>
    </dbReference>
    <dbReference type="PDB" id="1UWS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.95 A"/>
        <property type="chains" value="A/B=1-489"/>
    </dbReference>
    <dbReference type="PDB" id="1UWT">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.95 A"/>
        <property type="chains" value="A/B=1-489"/>
    </dbReference>
    <dbReference type="PDB" id="1UWU">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.95 A"/>
        <property type="chains" value="A/B=1-489"/>
    </dbReference>
    <dbReference type="PDB" id="2CEQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.14 A"/>
        <property type="chains" value="A/B=1-489"/>
    </dbReference>
    <dbReference type="PDB" id="2CER">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.29 A"/>
        <property type="chains" value="A/B=1-489"/>
    </dbReference>
    <dbReference type="PDB" id="4EAM">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A/B=1-489"/>
    </dbReference>
    <dbReference type="PDB" id="4EAN">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.75 A"/>
        <property type="chains" value="A/B=1-489"/>
    </dbReference>
    <dbReference type="PDB" id="5I3D">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.16 A"/>
        <property type="chains" value="A/B/C/D=1-489"/>
    </dbReference>
    <dbReference type="PDB" id="5IXE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.75 A"/>
        <property type="chains" value="A/B=1-489"/>
    </dbReference>
    <dbReference type="PDB" id="7UZ1">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.58 A"/>
        <property type="chains" value="A/B=1-489"/>
    </dbReference>
    <dbReference type="PDB" id="7UZ2">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.83 A"/>
        <property type="chains" value="A/B=1-489"/>
    </dbReference>
    <dbReference type="PDB" id="8Y9Q">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.96 A"/>
        <property type="chains" value="A/B=4-465"/>
    </dbReference>
    <dbReference type="PDBsum" id="1GOW"/>
    <dbReference type="PDBsum" id="1UWI"/>
    <dbReference type="PDBsum" id="1UWQ"/>
    <dbReference type="PDBsum" id="1UWR"/>
    <dbReference type="PDBsum" id="1UWS"/>
    <dbReference type="PDBsum" id="1UWT"/>
    <dbReference type="PDBsum" id="1UWU"/>
    <dbReference type="PDBsum" id="2CEQ"/>
    <dbReference type="PDBsum" id="2CER"/>
    <dbReference type="PDBsum" id="4EAM"/>
    <dbReference type="PDBsum" id="4EAN"/>
    <dbReference type="PDBsum" id="5I3D"/>
    <dbReference type="PDBsum" id="5IXE"/>
    <dbReference type="PDBsum" id="7UZ1"/>
    <dbReference type="PDBsum" id="7UZ2"/>
    <dbReference type="PDBsum" id="8Y9Q"/>
    <dbReference type="AlphaFoldDB" id="P22498"/>
    <dbReference type="SMR" id="P22498"/>
    <dbReference type="FunCoup" id="P22498">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3019"/>
    <dbReference type="CAZy" id="GH1">
        <property type="family name" value="Glycoside Hydrolase Family 1"/>
    </dbReference>
    <dbReference type="iPTMnet" id="P22498"/>
    <dbReference type="PaxDb" id="273057-SSO3019"/>
    <dbReference type="EnsemblBacteria" id="AAK43121">
        <property type="protein sequence ID" value="AAK43121"/>
        <property type="gene ID" value="SSO3019"/>
    </dbReference>
    <dbReference type="GeneID" id="1453040"/>
    <dbReference type="KEGG" id="sso:SSO3019"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3113"/>
    <dbReference type="eggNOG" id="arCOG05412">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001859_1_3_2"/>
    <dbReference type="InParanoid" id="P22498"/>
    <dbReference type="PhylomeDB" id="P22498"/>
    <dbReference type="BRENDA" id="3.2.1.23">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="3.2.1.55">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="3.2.1.B26">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="3.2.1.B34">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="P22498"/>
    <dbReference type="EvolutionaryTrace" id="P22498"/>
    <dbReference type="PRO" id="PR:P22498"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008378">
        <property type="term" value="F:galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053427">
        <property type="entry name" value="Beta-galactosidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041004">
        <property type="entry name" value="Beta_gal_BgaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF209">
        <property type="entry name" value="GALACTOLIPID GALACTOSYLTRANSFERASE SFR2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0326">Glycosidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000063867" description="Beta-galactosidase">
        <location>
            <begin position="1"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="387"/>
        </location>
    </feature>
    <feature type="site" description="Not N6-methylated">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="site" description="Not N6-methylated">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="site" description="Not N6-methylated">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="site" description="Not N6-methylated">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine; partial" evidence="3">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine" evidence="3">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine; partial" evidence="3">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine; partial" evidence="3">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine" evidence="3">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA72843." evidence="4" ref="1">
        <original>A</original>
        <variation>H</variation>
        <location>
            <position position="235"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="9"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="16"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="32"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="40"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="77"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="84"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="112"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="124"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="143"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="155"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="160"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="173"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="195"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="199"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="207"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="218"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="258"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="272"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="287"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="297"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="303"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="308"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="333"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="338"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="346"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="366"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="383"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="398"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="419"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="433"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="443"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="448"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="453"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="457"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="473"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="484"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="14660666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="1GOW"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="1UWI"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="5IXE"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="7UZ1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="7UZ2"/>
        </source>
    </evidence>
    <sequence length="489" mass="56692" checksum="B56F6CE8EE4A429D" modified="2001-06-20" version="2">MYSFPNSFRFGWSQAGFQSEMGTPGSEDPNTDWYKWVHDPENMAAGLVSGDLPENGPGYWGNYKTFHDNAQKMGLKIARLNVEWSRIFPNPLPRPQNFDESKQDVTEVEINENELKRLDEYANKDALNHYREIFKDLKSRGLYFILNMYHWPLPLWLHDPIRVRRGDFTGPSGWLSTRTVYEFARFSAYIAWKFDDLVDEYSTMNEPNVVGGLGYVGVKSGFPPGYLSFELSRRAMYNIIQAHARAYDGIKSVSKKPVGIIYANSSFQPLTDKDMEAVEMAENDNRWWFFDAIIRGEITRGNEKIVRDDLKGRLDWIGVNYYTRTVVKRTEKGYVSLGGYGHGCERNSVSLAGLPTSDFGWEFFPEGLYDVLTKYWNRYHLYMYVTENGIADDADYQRPYYLVSHVYQVHRAINSGADVRGYLHWSLADNYEWASGFSMRFGLLKVDYNTKRLYWRPSALVYREIATNGAITDEIEHLNSVPPVKPLRH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>P57084</accession>
    <name>NADM_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber>2.7.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>NMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0255</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="J. Bacteriol." volume="179" first="7718" last="7723">
            <title>Characterization of nicotinamide mononucleotide adenylyltransferase from thermophilic archaea.</title>
            <authorList>
                <person name="Raffaelli N."/>
                <person name="Pisani F.M."/>
                <person name="Lorenzi T."/>
                <person name="Emanuelli M."/>
                <person name="Amici A."/>
                <person name="Ruggieri S."/>
                <person name="Magni G."/>
            </authorList>
            <dbReference type="PubMed" id="9401030"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-33</scope>
        <scope>CHARACTERIZATION</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal NMN adenylyltransferase family.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40594" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.7.7.1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40594.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90167">
        <property type="entry name" value="C90167"/>
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    <dbReference type="RefSeq" id="WP_014511516.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P57084"/>
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    <dbReference type="STRING" id="273057.SSO0255"/>
    <dbReference type="PaxDb" id="273057-SSO0255"/>
    <dbReference type="EnsemblBacteria" id="AAK40594">
        <property type="protein sequence ID" value="AAK40594"/>
        <property type="gene ID" value="SSO0255"/>
    </dbReference>
    <dbReference type="GeneID" id="1455404"/>
    <dbReference type="KEGG" id="sso:SSO0255"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.249"/>
    <dbReference type="eggNOG" id="arCOG00972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108783_0_0_2"/>
    <dbReference type="InParanoid" id="P57084"/>
    <dbReference type="PhylomeDB" id="P57084"/>
    <dbReference type="BioCyc" id="MetaCyc:MONOMER-12229"/>
    <dbReference type="BRENDA" id="2.7.7.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02166">
        <property type="entry name" value="NMNAT_Archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00243">
        <property type="entry name" value="NMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006418">
        <property type="entry name" value="NMN_Atrans_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01527">
        <property type="entry name" value="arch_NMN_Atrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002243">
        <property type="entry name" value="PRK01153.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF0">
        <property type="entry name" value="BIFUNCTIONAL NMN ADENYLYLTRANSFERASE_NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342">
        <property type="entry name" value="PHOSPHOPANTETHEINE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000135005" description="Nicotinamide-nucleotide adenylyltransferase">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AA sequence." evidence="1" ref="2">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AA sequence." evidence="1" ref="2">
        <original>WS</original>
        <variation>IK</variation>
        <location>
            <begin position="23"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AA sequence." evidence="1" ref="2">
        <original>EL</original>
        <variation>PP</variation>
        <location>
            <begin position="30"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="172" mass="20096" checksum="03A4304EA0C09582" modified="2001-06-20" version="2">MSRGLYPGRFQPFHLGHLNVIKWSLERVDELIILVGSSQESHTVTNPFTAGERVEMIRNSLKDVGMDLSRIYIIPMPDILMNNIWAHYVSTYTPKFEVVFARNPLVVRIFKEAGYKVEIPPAFNREKYNSTYIRRLIILNDNWSELVPKPVYKYILEIKGDQRLREIVGTDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>P58084</accession>
    <name>RL29_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL29</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>50S ribosomal protein L29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rpl29</name>
        <name type="synonym">rpl29Ab</name>
        <name type="ordered locus">SSO0711</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90219">
        <property type="entry name" value="D90219"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991274.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58084"/>
    <dbReference type="SMR" id="P58084"/>
    <dbReference type="FunCoup" id="P58084">
        <property type="interactions" value="200"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO6397"/>
    <dbReference type="PaxDb" id="273057-SSO6397"/>
    <dbReference type="EnsemblBacteria" id="AAK41011">
        <property type="protein sequence ID" value="AAK41011"/>
        <property type="gene ID" value="SSO6397"/>
    </dbReference>
    <dbReference type="GeneID" id="1454978"/>
    <dbReference type="KEGG" id="sso:SSO6397"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.711"/>
    <dbReference type="eggNOG" id="arCOG00785">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158491_2_0_2"/>
    <dbReference type="InParanoid" id="P58084"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.310:FF:000001">
        <property type="entry name" value="50S ribosomal protein L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050063">
        <property type="entry name" value="Ribosomal_protein_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018254">
        <property type="entry name" value="Ribosomal_uL29_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_uL29_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10916">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35/50S RIBOSOMAL PROTEIN L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10916:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="Ribosomal protein L29 (L29p)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00579">
        <property type="entry name" value="RIBOSOMAL_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000130524" description="Large ribosomal subunit protein uL29">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="73" mass="8549" checksum="79477E46DDABCFF7" modified="2001-06-01" version="1">MTVDPEELRKMETGDLLKKLDELKLELIKLRVQSRMGTLKNTASIRNTRKDIARILTVLSEKKKVKREKVENK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2025-06-18" version="147" xmlns="https://uniprot.org/uniprot">
    <accession>P95961</accession>
    <name>SYFA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="1">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="1">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pheS</name>
        <name type="ordered locus">SSO0100</name>
        <name type="ORF">C04_022</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="1"/>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75389">
        <property type="entry name" value="S75389"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95961"/>
    <dbReference type="SMR" id="P95961"/>
    <dbReference type="FunCoup" id="P95961">
        <property type="interactions" value="341"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0100"/>
    <dbReference type="PaxDb" id="273057-SSO0100"/>
    <dbReference type="EnsemblBacteria" id="AAK40456">
        <property type="protein sequence ID" value="AAK40456"/>
        <property type="gene ID" value="SSO0100"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0100"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.97"/>
    <dbReference type="eggNOG" id="arCOG00410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025086_2_2_2"/>
    <dbReference type="InParanoid" id="P95961"/>
    <dbReference type="PhylomeDB" id="P95961"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00282">
        <property type="entry name" value="Phe_tRNA_synth_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022917">
        <property type="entry name" value="Phe_tRNA_ligase_alpha_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003210">
        <property type="entry name" value="PRK04172.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF40">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000126820" description="Phenylalanine--tRNA ligase alpha subunit">
        <location>
            <begin position="1"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="350"/>
            <end position="352"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with beta subunit</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00282"/>
        </source>
    </evidence>
    <sequence length="468" mass="54102" checksum="527E274CC79944E2" modified="1997-11-01" version="2">MISMLSENEAKILFFLKDLKKTNSVELAIKMGIPESSVLSLIELLREKGYVKTEVKSEKHYVLTEEGRKRKESGLPEDILINTLNGQEKDLNEIKNILNKDFNIAISWAKRKGLIDIKEGKVIPKVKTYMSSEYLALSNLERADSNTINLLKKRGLIEEKERKIVSVELIKEPKESEIGISNLNRELIISGEWKKYKLKKYNVEAFPPYYTISKKHYFREFLEKVKDIMISLGFKEINTGYIEMEFYNFDLLFQPQDHPAREIHDSFSVEGSGKIEDKDLLNNVKEIHEKFWKYEWKQDITLRLMLRSQTTATTARVLASRPKAPQKVFTLGKVFRPDAIDATHLIEFHQLDGVIIDNNFTFKELLGVLKEIFYRLGIKEVKFKPAYFPFTEPSVEAYGYLEKLGWVEMCGAGLLRPEILSSVGIDSTAGAWGIGIERLAMSFLNISDIRLLYSNNIEYIRDTKVKIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2026-01-28" version="152" xmlns="https://uniprot.org/uniprot">
    <accession>P95963</accession>
    <name>MAP2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methionine aminopeptidase</fullName>
            <shortName evidence="1">MAP</shortName>
            <shortName evidence="1">MetAP</shortName>
            <ecNumber evidence="1">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">map</name>
        <name type="ordered locus">SSO0098</name>
        <name type="ORF">C04024</name>
        <name type="ORF">C05_012</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="1"/>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75391">
        <property type="entry name" value="S75391"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988912.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95963"/>
    <dbReference type="SMR" id="P95963"/>
    <dbReference type="FunCoup" id="P95963">
        <property type="interactions" value="287"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0098"/>
    <dbReference type="PaxDb" id="273057-SSO0098"/>
    <dbReference type="EnsemblBacteria" id="AAK40454">
        <property type="protein sequence ID" value="AAK40454"/>
        <property type="gene ID" value="SSO0098"/>
    </dbReference>
    <dbReference type="GeneID" id="1453282"/>
    <dbReference type="GeneID" id="44129058"/>
    <dbReference type="KEGG" id="sso:SSO0098"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.95"/>
    <dbReference type="eggNOG" id="arCOG01001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_7_0_2"/>
    <dbReference type="InParanoid" id="P95963"/>
    <dbReference type="PhylomeDB" id="P95963"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01975">
        <property type="entry name" value="MetAP_2_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028595">
        <property type="entry name" value="MetAP_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0031">Aminopeptidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148981" description="Methionine aminopeptidase">
        <location>
            <begin position="1"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01975"/>
        </source>
    </evidence>
    <sequence length="301" mass="33252" checksum="3E156B383EF516F0" modified="1997-05-01" version="1">MTEDELNKLLLAGKIAAKARDEVSLDVKASAKVLDICEEVESIIIENKAFPSFPCNISINSEAAHYSPTINDEKRIPEGAVVKLDLGAHIDGFISDTAITISLDSRYQRLLDASKTALEAAITNFKAGLSIGEIGRVIEKVIRAQGYKPIRNLGGHLIRRYELHAGVFIPNVYERGLGVIQSDSVYAIEPFATDGGGEVVEGKSITIYSLKNPNIKGLSSRENELIDFIYTRFNYLPFSERWLKEFSTNVDELRNNIKNLVKKGALRGYPILIEIKKGVVSQFEHTVIVKGDSIIVSTKSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>P96036</accession>
    <name>SPT5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription elongation factor Spt5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">spt5</name>
        <name type="synonym">nusG</name>
        <name type="ordered locus">SSO0347</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="Biochim. Biophys. Acta" volume="1340" first="170" last="177">
            <title>Nucleotide sequence of a gene cluster encoding NusG and the L11-L1-L10-L12 ribosomal proteins from the thermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Geiger M."/>
                <person name="Groebner P."/>
                <person name="Piendl W."/>
            </authorList>
            <dbReference type="PubMed" id="9252104"/>
            <dbReference type="DOI" id="10.1016/S0167-4838(97)00073-3"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer composed of Spt4 and Spt5. Interacts with RNA polymerase (RNAP).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Spt5 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40676" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="U85262">
        <property type="protein sequence ID" value="AAB99523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40676.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90177">
        <property type="entry name" value="E90177"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990638.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P96036"/>
    <dbReference type="SMR" id="P96036"/>
    <dbReference type="STRING" id="273057.SSO0347"/>
    <dbReference type="PaxDb" id="273057-SSO0347"/>
    <dbReference type="EnsemblBacteria" id="AAK40676">
        <property type="protein sequence ID" value="AAK40676"/>
        <property type="gene ID" value="SSO0347"/>
    </dbReference>
    <dbReference type="GeneID" id="1455486"/>
    <dbReference type="KEGG" id="sso:SSO0347"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.337"/>
    <dbReference type="eggNOG" id="arCOG01920">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113589_0_0_2"/>
    <dbReference type="InParanoid" id="P96036"/>
    <dbReference type="PhylomeDB" id="P96036"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006354">
        <property type="term" value="P:DNA-templated transcription elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06091">
        <property type="entry name" value="KOW_NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09887">
        <property type="entry name" value="NGN_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="entry name" value="NusG, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00950">
        <property type="entry name" value="Spt5_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005100">
        <property type="entry name" value="NGN-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_uL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011590">
        <property type="entry name" value="Spt5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00405">
        <property type="entry name" value="KOW_elon_Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000113980" description="Transcription elongation factor Spt5">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="1">
        <location>
            <begin position="99"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="152" mass="16671" checksum="10A584E60D089915" modified="1997-05-01" version="1">MEKPNMRNYYAIKVVGGQEINVALMLEERIKTNNIKGIYAIIVPPNLKGYVVLEAEGLHIVKPLIAGIRNARGLAQGLLPRDEILKIVSRKTVGPTVKPGDVVEVISGPFRGTQAQVIRVEEAKGEVVLNILESAFPLQVTVPLDQIKVSKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1994-02-01" modified="2026-01-28" version="163" xmlns="https://uniprot.org/uniprot">
    <accession>Q06129</accession>
    <name>TRPG_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Anthranilate synthase component 2</fullName>
            <shortName>AS</shortName>
            <shortName>ASII</shortName>
            <ecNumber>4.1.3.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Anthranilate synthase, GATase component</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Anthranilate synthase, glutamine amidotransferase component</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">trpG</name>
        <name type="ordered locus">SSO0894</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1993" name="J. Bacteriol." volume="175" first="299" last="302">
            <title>Tryptophan biosynthesis genes trpEGC in the thermoacidophilic archaebacterium Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Tutino M.L."/>
                <person name="Scarano G."/>
                <person name="Marino G."/>
                <person name="Sannia G."/>
                <person name="Cubellis M.V."/>
            </authorList>
            <dbReference type="PubMed" id="8416906"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1999" name="Proc. Natl. Acad. Sci. U.S.A." volume="96" first="9479" last="9484">
            <title>The crystal structure of anthranilate synthase from Sulfolobus solfataricus: functional implications.</title>
            <authorList>
                <person name="Knoechel T."/>
                <person name="Ivens A."/>
                <person name="Hester G."/>
                <person name="Gonzalez A."/>
                <person name="Bauerle R."/>
                <person name="Wilmanns M."/>
                <person name="Kirschner K."/>
                <person name="Jansonius J.N."/>
            </authorList>
            <dbReference type="PubMed" id="10449718"/>
            <dbReference type="DOI" id="10.1073/pnas.96.17.9479"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27"/>
    <dbReference type="EMBL" id="M98048">
        <property type="protein sequence ID" value="AAA73380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Z50014">
        <property type="protein sequence ID" value="CAA90312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99240">
        <property type="entry name" value="A99240"/>
    </dbReference>
    <dbReference type="PIR" id="B40635">
        <property type="entry name" value="B40635"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992310.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1QDL">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="B=1-195"/>
    </dbReference>
    <dbReference type="PDBsum" id="1QDL"/>
    <dbReference type="AlphaFoldDB" id="Q06129"/>
    <dbReference type="SMR" id="Q06129"/>
    <dbReference type="DIP" id="DIP-6203N"/>
    <dbReference type="FunCoup" id="Q06129">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="IntAct" id="Q06129">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="MINT" id="Q06129"/>
    <dbReference type="STRING" id="273057.SSO0894"/>
    <dbReference type="PaxDb" id="273057-SSO0894"/>
    <dbReference type="EnsemblBacteria" id="AAK41176">
        <property type="protein sequence ID" value="AAK41176"/>
        <property type="gene ID" value="SSO0894"/>
    </dbReference>
    <dbReference type="GeneID" id="1455143"/>
    <dbReference type="KEGG" id="sso:SSO0894"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.897"/>
    <dbReference type="eggNOG" id="arCOG00086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_1_3_2"/>
    <dbReference type="InParanoid" id="Q06129"/>
    <dbReference type="PhylomeDB" id="Q06129"/>
    <dbReference type="BioCyc" id="MetaCyc:MONOMER-3604"/>
    <dbReference type="BRENDA" id="4.1.3.27">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q06129"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000003">
        <property type="entry name" value="Anthranilate synthase component II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF4">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000056900" description="Anthranilate synthase component 2">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="2">
        <location>
            <begin position="3"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for GATase activity" evidence="2">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="2">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="2">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="54"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="137"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA73380/CAA90312." evidence="4" ref="1">
        <original>I</original>
        <variation>L</variation>
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="3"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="27"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="33"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="38"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="47"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="61"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="67"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="75"/>
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        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="80"/>
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        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="85"/>
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        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="97"/>
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        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="122"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="128"/>
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        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="159"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="10449718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="10449718"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="1QDL"/>
        </source>
    </evidence>
    <sequence length="195" mass="21909" checksum="155B62933E9CD33E" modified="2001-06-01" version="2">MDLTLIIDNYDSFVYNIAQIVGELGSYPIVIRNDEISIKGIERIDPDRIIISPGPGTPEKREDIGVSLDVIKYLGKRTPILGVCLGHQAIGYAFGAKIRRARKVFHGKISNIILVNNSPLSLYYGIAKEFKATRYHSLVVDEVHRPLIVDAISAEDNEIMAIHHEEYPIYGVQFHPESVGTSLGYKILYNFLNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX96</accession>
    <name>Q7LX96_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1234</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74012">
        <property type="entry name" value="S74012"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923771.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX96"/>
    <dbReference type="PaxDb" id="273057-SSO2099"/>
    <dbReference type="EnsemblBacteria" id="AAK42278">
        <property type="protein sequence ID" value="AAK42278"/>
        <property type="gene ID" value="SSO2099"/>
    </dbReference>
    <dbReference type="GeneID" id="1453605"/>
    <dbReference type="KEGG" id="sso:SSO2099"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2181"/>
    <dbReference type="eggNOG" id="arCOG07925">
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    <dbReference type="HOGENOM" id="CLU_075479_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX96"/>
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        <property type="component" value="Chromosome"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="319" mass="36729" checksum="46B1B459BD6F435E" modified="2011-05-31" version="1">MGVEGSTMARTLRHIPNLMYYPLPPIEDMPWREKWLTEIKPVLDAMDLERVLGEGALVYLKLLIVMVLYSCSYRDAVKMVNVNVVVAWFVGRKVGKSTLHDFVGRLYGVRKKLLEISFKLEEKCLPSYLPASAHLVDFMAWLVDSFLLDLPPGKRSVETFREKAELERREGNLERAGKLLSLGRTKRRFEGRWTKKRGVSHYGLKAVAVISVSLFVRSITLKPANFSDKRFKSPLKGIKIADRGFSPSPTQLIAREKPFTTLRAHVEFFGTYLNAFWRPYGTTTWRNDVFLHVLGVIYNIKIFLAIQRRTPPERRAVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXF4</accession>
    <name>Q7LXF4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0823</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90232">
        <property type="entry name" value="H90232"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923114.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXF4"/>
    <dbReference type="STRING" id="273057.SSO0823"/>
    <dbReference type="PaxDb" id="273057-SSO0823"/>
    <dbReference type="EnsemblBacteria" id="AAK41119">
        <property type="protein sequence ID" value="AAK41119"/>
        <property type="gene ID" value="SSO0823"/>
    </dbReference>
    <dbReference type="GeneID" id="71027419"/>
    <dbReference type="KEGG" id="sso:SSO0823"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.835"/>
    <dbReference type="eggNOG" id="arCOG01417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1682786_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXF4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="156" mass="18594" checksum="88A08ED71C5519B0" modified="2011-05-31" version="1">MCQIIAQKILVLMDPQPEGGSAYVEAQISRRLLNKFGDNIKVAYKINDWSYIDKMLNELKNDNIKMIFDLNYWPINYHQIKFFKNNFKINNYHILLDHSYYYSRKLKVRSALLLQGMGLHASRLGIPYNLIKYLKIRKNFPIDIFLSTRNKLIYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXR4</accession>
    <name>Q7LXR4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="7">ATase</shortName>
            <ecNumber evidence="7">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
            <shortName evidence="7">GPATase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">purF-1</name>
        <name evidence="7" type="synonym">purF</name>
        <name evidence="13" type="ordered locus">SSO0632</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7 10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7 11">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7 8">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90210">
        <property type="entry name" value="F90210"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991166.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXR4"/>
    <dbReference type="FunCoup" id="Q7LXR4">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0632"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="PaxDb" id="273057-SSO0632"/>
    <dbReference type="EnsemblBacteria" id="AAK40941">
        <property type="protein sequence ID" value="AAK40941"/>
        <property type="gene ID" value="SSO0632"/>
    </dbReference>
    <dbReference type="GeneID" id="1454908"/>
    <dbReference type="GeneID" id="44129632"/>
    <dbReference type="KEGG" id="sso:SSO0632"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.637"/>
    <dbReference type="eggNOG" id="arCOG00093">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022389_3_3_2"/>
    <dbReference type="InParanoid" id="Q7LXR4"/>
    <dbReference type="PhylomeDB" id="Q7LXR4"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 7 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 11" id="KW-0408">Iron</keyword>
    <keyword evidence="7 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="12">
        <location>
            <begin position="8"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7 9">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="376"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAK40941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="450" mass="50494" checksum="5B0FACBEC8135B60" modified="2011-05-31" version="1">MDSIRDKCGVFAVSSPKEVNIQLVVEGIRLLQHRGQESAGIAYAENGEILTIKGSGLVDEVFKENLNKFIKNGIGHVRYSTSGKSSIEEAQPLGDSKIVVAFNGTISNYYQFGSFRVDTEFIYKFFKQKLAFRSIPDTVKEFMKVVDGGYSVAILLNDEEIVVFRDPKGFHPVVLGFLEGSLIVSSEDSVIRQLGGSVLKHILPSEMIIMKNGKILYDKVIYEEKNYATCSFEYIYFARPDSNIDGHSVYLARIRLGELLAEKHPANGDIVVPVPDSSRPIALGFSRKSHIPLEEPLVRTISSVRSFIMPTQDKRNEVLEEKFGVVADAVRGKRIVLIDDSIVRGNTMKRIITMLRKAGAKEIHVRIGSPMIKYPCYMGIDFPKREELIAHNKSEKEIGNELNADSIEFLSVEEMLQGIGRRDLCNACFSGIYPLKFNYNSTELERIFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXT3</accession>
    <name>Q7LXT3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD(P)/FAD-dependent oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0584</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90205">
        <property type="entry name" value="B90205"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991100.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXT3"/>
    <dbReference type="STRING" id="273057.SSO0584"/>
    <dbReference type="PaxDb" id="273057-SSO0584"/>
    <dbReference type="EnsemblBacteria" id="AAK40897">
        <property type="protein sequence ID" value="AAK40897"/>
        <property type="gene ID" value="SSO0584"/>
    </dbReference>
    <dbReference type="GeneID" id="1454864"/>
    <dbReference type="KEGG" id="sso:SSO0584"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.593"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083533_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXT3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF21">
        <property type="entry name" value="DEHYDROGENASE (FLAVOPROTEIN)-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="33451" checksum="CA4549C7B99440AE" modified="2011-05-31" version="1">MDFDPIVFDRRRFPGKKCTGIISRRTFLTLGVSREFIDREFKTIEIRYDRKYTIYISTDVIRLNREKLEIWLDSEVKTKRPRDAIINGNTVISGNEKYEGIVVDSSGWKGNAKWIKAIEYLTEPVNEENIIVYIDSKNVGGFSWIVPLPYGTLVGAISYNDPKVFLPNINKRVFDTHGGAIPRVLPISSIKTLKFGDSTGLIKTFTGGGMFGIASLLYPLVNGIRNGKFSEYYSMYKILAKEVRKQYYITRLLESTWRFLPILFKLYNDKTLNISEEFDLHSLLVSRFPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-03-27" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXV4</accession>
    <name>Q7LXV4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0545</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90200">
        <property type="entry name" value="H90200"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXV4"/>
    <dbReference type="STRING" id="273057.SSO0545"/>
    <dbReference type="PaxDb" id="273057-SSO0545"/>
    <dbReference type="EnsemblBacteria" id="AAK40863">
        <property type="protein sequence ID" value="AAK40863"/>
        <property type="gene ID" value="SSO0545"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0545"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.549"/>
    <dbReference type="HOGENOM" id="CLU_999726_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXV4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="286" mass="32864" checksum="8C478AF9A48D4AB8" modified="2011-05-31" version="1">MKYSTQIKVEISSKVEYITGKNLIPKIKSIFSNIEIKIENSELEDKQFKDFLKSYYNFYDSSKIDTIFIIEVNLIDETYRGLGIGSYDTKTNISSLYFEWESTTKYEVQAASGGSIICWDYSTYSKAYHGAVNISSVAGTLEGFPLEANVTYLTLNDEGEIVFQGYQKGIVKTINGIRVGKVKLVGLIDEKFRLNTSPGYAVYLTQKNDNIMYGVYNQIQVDPMGRMYYETAIREYKIYSGRKLPFDEVMIFKYKDANFNQIKSNKRIDSMSHIAYYMPAKDPYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TY0</accession>
    <name>Q97TY0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycosyltransferase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO12256</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90511">
        <property type="entry name" value="A90511"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TY0"/>
    <dbReference type="FunCoup" id="Q97TY0">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO12256"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO12256"/>
    <dbReference type="EnsemblBacteria" id="AAK43339">
        <property type="protein sequence ID" value="AAK43339"/>
        <property type="gene ID" value="SSO12256"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO12256"/>
    <dbReference type="eggNOG" id="arCOG01389">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="InParanoid" id="Q97TY0"/>
    <dbReference type="PhylomeDB" id="Q97TY0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06423">
        <property type="entry name" value="CESA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630:SF1">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13641">
        <property type="entry name" value="Glyco_tranf_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="349" mass="39922" checksum="39CA5980696421CC" modified="2001-10-01" version="1">MIVPVKNEERVLPRLLDRLVNLEYDKSKYEIIVVEDGSTDRTFQICKEYEIKYNNLIRCYSLPRANVPNGKSRALNFALRISKGEIIGIFDGDTVPRLDILEYVEPKFEDITVGAVQGKLVPINVRESVTSRLAAIEELIYEYSIAGRAKVGLFVPIEGTCSFIRKSIIMELGGWNEYSLTEDLDISLKIVNKGCKIVYSPTTISWREVPVSLRVLIRQRLRWYRGHLEVQLGKLRKIDLRIIDGILIVLTPFFMVLNLVNYSLVLVYSSSLYIVAASLVSLASLLSLLLIILIARRHMIEYFYMIPSFVYMNFIVALNFTAIFLELIRAPRVWVKTERSAKVTGEVMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U45</accession>
    <name>Q97U45_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90502">
        <property type="entry name" value="F90502"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991712.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U45"/>
    <dbReference type="PaxDb" id="273057-SSO3179"/>
    <dbReference type="EnsemblBacteria" id="AAK43277">
        <property type="protein sequence ID" value="AAK43277"/>
        <property type="gene ID" value="SSO3179"/>
    </dbReference>
    <dbReference type="GeneID" id="1453196"/>
    <dbReference type="KEGG" id="sso:SSO3179"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3283"/>
    <dbReference type="eggNOG" id="arCOG08488">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_902003_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U45"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="259" mass="30164" checksum="FEF191E2E8391CE9" modified="2001-10-01" version="1">MPIKGKRKYKVEFDDYTLTSVNGEISLINELGSKVDFSKLEIGDFLANKINSDRYFYYKIIPLSKYSDIVTYSLIDSIVKVGGFYGDLLEACYLKDSKSGSADYNYLKYAFDKISILNIKIYDTISDEAVKKLIDIYGWDFIKIYENLRSLFRVSAEKAYNKVIEKLDSRKELDAFMVYIIYSYNKNKAKEILEKTVITPNLDYDYEYIIPRWGLVGIDDNIIKMFNEVGVNVHKDSLRFNEIKILLTKINNEKVKLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U86</accession>
    <name>Q97U86_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Regulatory protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90497">
        <property type="entry name" value="D90497"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990919.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U86"/>
    <dbReference type="FunCoup" id="Q97U86">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3136"/>
    <dbReference type="PaxDb" id="273057-SSO3136"/>
    <dbReference type="EnsemblBacteria" id="AAK43235">
        <property type="protein sequence ID" value="AAK43235"/>
        <property type="gene ID" value="SSO3136"/>
    </dbReference>
    <dbReference type="GeneID" id="1453154"/>
    <dbReference type="KEGG" id="sso:SSO3136"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3241"/>
    <dbReference type="eggNOG" id="arCOG00774">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094876_0_1_2"/>
    <dbReference type="InParanoid" id="Q97U86"/>
    <dbReference type="PhylomeDB" id="Q97U86"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03451">
        <property type="entry name" value="FkbR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048274">
        <property type="entry name" value="MC_hydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052342">
        <property type="entry name" value="MCH/BMMD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43664:SF1">
        <property type="entry name" value="BETA-METHYLMALYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43664">
        <property type="entry name" value="MONOAMINE OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19315">
        <property type="entry name" value="MC_hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="161" mass="18420" checksum="089FA112FA7939BB" modified="2001-10-01" version="1">MSQEESGPFFEDFKVGQKFKSKVGRTITDVDNIWFTLLTNNSNQIHFNKDYTEKYFPGEPFNGRLVVNGFLTLTIVAGLLVEQTSQNGFMLGIENVKFLHPVFSGDTIYAEAEVIKVRESKSRPGFGIVKIRSYGYNQKGEKLIEFDRVFMVRKRGAKWSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U88</accession>
    <name>Q97U88_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO3132</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90497">
        <property type="entry name" value="B90497"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989958.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U88"/>
    <dbReference type="FunCoup" id="Q97U88">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3132"/>
    <dbReference type="PaxDb" id="273057-SSO3132"/>
    <dbReference type="EnsemblBacteria" id="AAK43233">
        <property type="protein sequence ID" value="AAK43233"/>
        <property type="gene ID" value="SSO3132"/>
    </dbReference>
    <dbReference type="GeneID" id="1453152"/>
    <dbReference type="KEGG" id="sso:SSO3132"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3238"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_5_4_2"/>
    <dbReference type="InParanoid" id="Q97U88"/>
    <dbReference type="PhylomeDB" id="Q97U88"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016790">
        <property type="term" value="F:thiolester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06262">
        <property type="entry name" value="metallo-hydrolase-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051453">
        <property type="entry name" value="MBL_Glyoxalase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46233">
        <property type="entry name" value="HYDROXYACYLGLUTATHIONE HYDROLASE GLOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46233:SF3">
        <property type="entry name" value="HYDROXYACYLGLUTATHIONE HYDROLASE GLOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="5">
        <location>
            <begin position="12"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="209" mass="23000" checksum="AA863D463AB056A6" modified="2001-10-01" version="1">MIVKNFITGPLATNSYLVIAGKEGVIIDAGGDMSELIQTVRKEKINIRYIIATHGHFDHIMGVNQIKRDFPSSTFLINEKDLGLLKRASSMAQSFLNLLISDVTKPDGFVKEGDEIELGGEKLKIIETPGHTMGSICIIANGYIFTGDTLFYGTVGRTDLGGSEKLLRESLEKLKKLPDEIIVYPGHGPFTVLGYEKVKNPFLTMDILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UC0</accession>
    <name>Q97UC0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH bat-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90493">
        <property type="entry name" value="B90493"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989122.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UC0"/>
    <dbReference type="PaxDb" id="273057-SSO3097"/>
    <dbReference type="EnsemblBacteria" id="AAK43201">
        <property type="protein sequence ID" value="AAK43201"/>
        <property type="gene ID" value="SSO3097"/>
    </dbReference>
    <dbReference type="GeneID" id="7808541"/>
    <dbReference type="KEGG" id="sso:SSO3097"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3203"/>
    <dbReference type="eggNOG" id="arCOG02272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101251_2_0_2"/>
    <dbReference type="InParanoid" id="Q97UC0"/>
    <dbReference type="PhylomeDB" id="Q97UC0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH bat-type" evidence="1">
        <location>
            <begin position="162"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="220" mass="25837" checksum="1BB344F0DA7A68A7" modified="2001-10-01" version="1">MIKSYTIVLSHDDWSRYTERFNEEELYVKVVRRIPSLDGLTENVMGIIYAKDKEFYRQIIRMLSRNETTIDFRIIDEYKNKSNIRAIYASIESLKGRIAERLLKDCRIFSMSETIYEGMERWNVIADSENFIKIIPEVKELTYEFKIIEESSNPLLSVKEELTPKELSILKLAFDKGYFETPKRIGLEELANELGISKPAIMQVLRKAIAKLTEKSIKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UC1</accession>
    <name>Q97UC1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99493">
        <property type="entry name" value="A99493"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989121.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UC1"/>
    <dbReference type="STRING" id="273057.SSO3095"/>
    <dbReference type="PaxDb" id="273057-SSO3095"/>
    <dbReference type="EnsemblBacteria" id="AAK43200">
        <property type="protein sequence ID" value="AAK43200"/>
        <property type="gene ID" value="SSO3095"/>
    </dbReference>
    <dbReference type="GeneID" id="1453119"/>
    <dbReference type="KEGG" id="sso:SSO3095"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3202"/>
    <dbReference type="eggNOG" id="arCOG08496">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_493173_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UC1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="552" mass="62715" checksum="E63EEC9E033E7A5F" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UC7</accession>
    <name>Q97UC7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0033 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90492">
        <property type="entry name" value="C90492"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989114.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UC7"/>
    <dbReference type="STRING" id="273057.SSO3088"/>
    <dbReference type="PaxDb" id="273057-SSO3088"/>
    <dbReference type="EnsemblBacteria" id="AAK43194">
        <property type="protein sequence ID" value="AAK43194"/>
        <property type="gene ID" value="SSO3088"/>
    </dbReference>
    <dbReference type="GeneID" id="1453113"/>
    <dbReference type="KEGG" id="sso:SSO3088"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3196"/>
    <dbReference type="eggNOG" id="arCOG02074">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_856905_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UC7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00291">
        <property type="entry name" value="SirA_YedF_YeeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="entry name" value="TusA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279:SF18">
        <property type="entry name" value="SULFUR CARRIER PROTEIN MJ0990-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SirA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="1">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="324" mass="38040" checksum="861EF3865FDB448E" modified="2001-10-01" version="1">MIEETTLIYAEDFKSLLDLENSYKLYKLSNIKKLDFGYICYLTIFRLKVECICKPRKDGLDIIEKNGRFIINITFQKESKERINVKISYRGILEKLLSSIANSIRKNLEEYSKYLIGKQKVENNLRISTLKPDKVVDLRGEECPVPEITLKRELMKANRGEIIEALTDNPAAVAHTIPEIIKLFNCRYEVLKYEDYVSFRILVLSNTINTDEYVKVIKEFNEARIRELIRDKKFMSFLYTYFVKFHKVEKVNDFKNYRFHCEKDICLVSSAPLGRGWLFTGLIKSNKMVCARIDTENETLLDYQALEYLKKLAGETNVMYLSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UD4</accession>
    <name>Q97UD4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter, ATP binding protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO3083</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90491">
        <property type="entry name" value="D90491"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989102.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UD4"/>
    <dbReference type="FunCoup" id="Q97UD4">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3083"/>
    <dbReference type="PaxDb" id="273057-SSO3083"/>
    <dbReference type="EnsemblBacteria" id="AAK43187">
        <property type="protein sequence ID" value="AAK43187"/>
        <property type="gene ID" value="SSO3083"/>
    </dbReference>
    <dbReference type="GeneID" id="1453106"/>
    <dbReference type="KEGG" id="sso:SSO3083"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3189"/>
    <dbReference type="eggNOG" id="arCOG00196">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q97UD4"/>
    <dbReference type="PhylomeDB" id="Q97UD4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051782">
        <property type="entry name" value="ABC_Transporter_VariousFunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939:SF1">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="291" mass="32517" checksum="176F46DBD417B9FF" modified="2001-10-01" version="1">MGVIDAINLTKRYGDIEVLHGLTFSIEEKSITLVIGPNGAGKSTLLKIIGGLINRTSGYISVLGEDPWNSNKIPRKVGIILDRPFLPPYLTVIDIVKEVSSEFSYPLSDAKRLLEDFNLVHFMKNKVRELSAGTKQKLQIVFSLIKNPEIIIADEPTANLDITSRYEVYNTFAVLREKMGLTVLISSHIPAEILAFSTHVLAINNGIMKFFGKVDKLLRNNFLEEFYIVVDNIGKALEALKDRRIEVIGNQIRISGNLAEISKILSNAGVRIFYVRNSIIDKSIKGETGWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UI4</accession>
    <name>Q97UI4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Beta-xylosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO3032</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90484">
        <property type="entry name" value="G90484"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924101.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UI4"/>
    <dbReference type="SMR" id="Q97UI4"/>
    <dbReference type="FunCoup" id="Q97UI4">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3032"/>
    <dbReference type="CAZy" id="GH3">
        <property type="family name" value="Glycoside Hydrolase Family 3"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3032"/>
    <dbReference type="EnsemblBacteria" id="AAK43134">
        <property type="protein sequence ID" value="AAK43134"/>
        <property type="gene ID" value="SSO3032"/>
    </dbReference>
    <dbReference type="GeneID" id="1453053"/>
    <dbReference type="KEGG" id="sso:SSO3032"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3129"/>
    <dbReference type="eggNOG" id="arCOG04634">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004542_5_1_2"/>
    <dbReference type="InParanoid" id="Q97UI4"/>
    <dbReference type="PhylomeDB" id="Q97UI4"/>
    <dbReference type="BRENDA" id="3.2.1.37">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000011">
        <property type="entry name" value="Glycosyl hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000495">
        <property type="entry name" value="Periplasmic beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1700:FF:000016">
        <property type="entry name" value="Periplasmic beta-glucosidase, xylosidase/arabinosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051915">
        <property type="entry name" value="Cellulose_Degrad_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
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        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30620:SF123">
        <property type="entry name" value="BETA-XYLOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30620">
        <property type="entry name" value="PERIPLASMIC BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type III-like" evidence="3">
        <location>
            <begin position="655"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="754" mass="83619" checksum="01EBEC82CF07EC9E" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UL7</accession>
    <name>Q97UL7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2987</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90479">
        <property type="entry name" value="E90479"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992638.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UL7"/>
    <dbReference type="PaxDb" id="273057-SSO2987"/>
    <dbReference type="EnsemblBacteria" id="AAK43092">
        <property type="protein sequence ID" value="AAK43092"/>
        <property type="gene ID" value="SSO2987"/>
    </dbReference>
    <dbReference type="GeneID" id="1453011"/>
    <dbReference type="KEGG" id="sso:SSO2987"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3081"/>
    <dbReference type="eggNOG" id="arCOG05974">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1381430_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UL7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="202" mass="22792" checksum="89D95388A3C1E799" modified="2001-10-01" version="1">MEFLSKRERLVLVAIAQAGPSGTTYSQLSALGYILSRNTIQETIENLYLKGYINVLKDGDEIRYIASKSVRNAIINLEFHKYRLAKNLSELKKKVEDISKMEKQQQAVAFKGVLKDLLSSISSSIISLLDDMPALTLPEYTEVLENLYREFLSKLSPLMQESMTEDEINAFISLVSKYRGEKEAEEIKSTLQKIKGGAESQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UN0</accession>
    <name>Q97UN0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Quinol oxidase-2, subunit I/III, cytochrome aa3 (SoxM)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">soxM</name>
        <name evidence="9" type="ordered locus">SSO2973</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90477">
        <property type="entry name" value="G90477"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UN0"/>
    <dbReference type="FunCoup" id="Q97UN0">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2973"/>
    <dbReference type="PaxDb" id="273057-SSO2973"/>
    <dbReference type="EnsemblBacteria" id="AAK43078">
        <property type="protein sequence ID" value="AAK43078"/>
        <property type="gene ID" value="SSO2973"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2973"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3064"/>
    <dbReference type="eggNOG" id="arCOG01237">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011899_7_1_2"/>
    <dbReference type="InParanoid" id="Q97UN0"/>
    <dbReference type="PhylomeDB" id="Q97UN0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00919">
        <property type="entry name" value="Heme_Cu_Oxidase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00386">
        <property type="entry name" value="Heme_Cu_Oxidase_III_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.210.10:FF:000017">
        <property type="entry name" value="Cytochrome c oxidase subunit I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.80">
        <property type="entry name" value="Cytochrome c oxidase, subunit III, four-helix bundle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023615">
        <property type="entry name" value="Cyt_c_Oxase_su1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000298">
        <property type="entry name" value="Cyt_c_oxidase-like_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035973">
        <property type="entry name" value="Cyt_c_oxidase_su3-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013833">
        <property type="entry name" value="Cyt_c_oxidase_su3_a-hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422:SF18">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00510">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="Cytochrome c oxidase subunit I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81452">
        <property type="entry name" value="Cytochrome c oxidase subunit III-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00077">
        <property type="entry name" value="COX1_CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50253">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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        <location>
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        <location>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000370"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50253"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAK43078.1"/>
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        <source>
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    </evidence>
    <sequence length="790" mass="87557" checksum="6BB7A1EAA6FB66D5" modified="2001-10-01" version="1"> </sequence>
</entry>
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    <accession>Q97UV9</accession>
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        <name evidence="2" type="ordered locus">SSO2885</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAK42991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="304" mass="35080" checksum="437894F5B84BEF00" modified="2001-10-01" version="1">MEKKPLGWTNEKISPLILGSWEFGTPSIIDENNAIKAIQRAIELGINAIDTAESYGNGLSETIIGRAISKFKREDLFIITKVSIDHLRYDDVLKASEGSLKRLNTSYIDLYLVHWPNHYVPIRETAKAMERLFNEGKIRYVGLSNFSLPLMREFREHLSKTDVAANELHYNVLFRDVEKEVLPYMLRENIPLLAYDALGLGYLIGRKEVRNEYRWYVLARETYIKNLEPLIEEISSIAKELGKTPAQVVLNWLVSKDNVFAIFNTTKEDHLKENLGSLGWSLRDSDLRRLDEAVKKVTIDYFAR</sequence>
</entry>
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    <accession>Q97UW1</accession>
    <name>Q97UW1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Second ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">SSO2883</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="F90466"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UW1"/>
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    <dbReference type="EnsemblBacteria" id="AAK42989">
        <property type="protein sequence ID" value="AAK42989"/>
        <property type="gene ID" value="SSO2883"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2883"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2969"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074024_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UW1"/>
    <dbReference type="PhylomeDB" id="Q97UW1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Transposase_11"/>
    </dbReference>
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        <property type="entry name" value="transpos_ISH3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DDE_Tnp_1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
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            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="41"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="296" mass="34382" checksum="0E9E9D422105FD2B" modified="2001-10-01" version="1">MSPQTVRNYVEEQGTEVIEKLLESMRRISMEILKGVKEVDISIDWTTKTWYGKPVEGLGSSAKGNSWNYATATTKYQNMVLLLAFVPQVNGMSKDEIVKLLMEQIVGMGLKVGLVTLDAGFYTVEVLKFISQFKFVIGVPVGDVKIYEEFDGEYTTNSKRHKKEEQVKFRLLVYGKESLRRGEDRGVLREATNLDLPKREVLKLYNKVRSPIETSYRNIKAFLPFTSSTKFIFRELIFVLAMIFYSLYTVFKNVMTREEFRLLLILCFLDDLSDLKDFIFNLEETLINTIDLFLWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UX5</accession>
    <name>Q97UX5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
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        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
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            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90464">
        <property type="entry name" value="F90464"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989835.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UX5"/>
    <dbReference type="STRING" id="273057.SSO2866"/>
    <dbReference type="PaxDb" id="273057-SSO2866"/>
    <dbReference type="EnsemblBacteria" id="AAK42973">
        <property type="protein sequence ID" value="AAK42973"/>
        <property type="gene ID" value="SSO2866"/>
    </dbReference>
    <dbReference type="GeneID" id="7807704"/>
    <dbReference type="KEGG" id="sso:SSO2866"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2952"/>
    <dbReference type="eggNOG" id="arCOG06953">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="113" mass="12527" checksum="C99D0336811B0DE8" modified="2001-10-01" version="1">MNIWLVSAGIAILQTLLGNIIVFYNSPYLLLLHVFVAIILLALAIYGYFRVKLQMEKRILAGNIGLIVITGALGYLYTINASPIISIIHLLLAIGIVSNFSVLYGFERGQKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V56</accession>
    <name>Q97V56_SACS2</name>
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        <submittedName>
            <fullName evidence="2">Dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="B90454"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990217.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V56"/>
    <dbReference type="FunCoup" id="Q97V56">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2779"/>
    <dbReference type="PaxDb" id="273057-SSO2779"/>
    <dbReference type="EnsemblBacteria" id="AAK42889">
        <property type="protein sequence ID" value="AAK42889"/>
        <property type="gene ID" value="SSO2779"/>
    </dbReference>
    <dbReference type="GeneID" id="1452808"/>
    <dbReference type="KEGG" id="sso:SSO2779"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2861"/>
    <dbReference type="eggNOG" id="arCOG01619">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_3_2"/>
    <dbReference type="InParanoid" id="Q97V56"/>
    <dbReference type="PhylomeDB" id="Q97V56"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19072">
        <property type="entry name" value="AKR_AKR3F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43638:SF3">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43638">
        <property type="entry name" value="OXIDOREDUCTASE, ALDO/KETO REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="14"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="292" mass="33603" checksum="DD83D9230DB73FC3" modified="2001-10-01" version="1">MEDVKKFKYFTVSPLAFGTWRIGGGYWYSSHDRDNEWVGAIRRAIELGLRVIDTAEMYGNGHAEELVGEAIKEFSREELFIVSKVWPSHADYDNVIKSAKNSSRRLGTYIDLYLLHAPSRVPICKTIRAFEKLVDDGVIRFFGLSNFDVDQIESARECVSKYEIVAIQNHYSLLSRSDERRALAYAEKNGLMYMAYTPLENGILARNEFLANIGKKYNKTATQVALNWYITGKNSLIPIVKASKIPHIEENAGAMGWRLSEEDWRAIDEHFREKSYFLDKFVSSLKSMRPWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V87</accession>
    <name>Q97V87_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2748</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90450">
        <property type="entry name" value="C90450"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990265.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V87"/>
    <dbReference type="STRING" id="273057.SSO2748"/>
    <dbReference type="PaxDb" id="273057-SSO2748"/>
    <dbReference type="EnsemblBacteria" id="AAK42858">
        <property type="protein sequence ID" value="AAK42858"/>
        <property type="gene ID" value="SSO2748"/>
    </dbReference>
    <dbReference type="GeneID" id="1452777"/>
    <dbReference type="KEGG" id="sso:SSO2748"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2827"/>
    <dbReference type="eggNOG" id="arCOG03861">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1056018_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V87"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="269" mass="30447" checksum="DE96D89C1EC33B16" modified="2001-10-01" version="1">MPYAEEMEVKASRSQLLSFFIDPVRFAGILGHLMIVNIFDKAESKFVTMGSLKKPENKFKVLYLIGDPESRLITFSGIMEGPTLTFNTITYKGEGDNGKLTWRIDLILTELGKLTRMKVAADVKQSYGFLDRVRIGDFDFATHLVKEHVIPFVKFYFKPSILEETPVLNEVFRFRGSVEEVILKLREVIVNIKQGGIIILGDDYNIITSIVNGEVSHAQINNMDVPGSEVFAYLLKASGNVELIAYDISLDDLIFKNLKRRLEEVKAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VE8</accession>
    <name>Q97VE8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="E90442"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988598.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VE8"/>
    <dbReference type="STRING" id="273057.SSO2678"/>
    <dbReference type="PaxDb" id="273057-SSO2678"/>
    <dbReference type="EnsemblBacteria" id="AAK42796">
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        <property type="gene ID" value="SSO2678"/>
    </dbReference>
    <dbReference type="GeneID" id="1452715"/>
    <dbReference type="KEGG" id="sso:SSO2678"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2760"/>
    <dbReference type="eggNOG" id="arCOG08524">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_538226_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VE8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="512" mass="57895" checksum="7F9898554D0641F4" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VH8</accession>
    <name>Q97VH8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90438">
        <property type="entry name" value="G90438"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VH8"/>
    <dbReference type="STRING" id="273057.SSO2645"/>
    <dbReference type="PaxDb" id="273057-SSO2645"/>
    <dbReference type="EnsemblBacteria" id="AAK42766">
        <property type="protein sequence ID" value="AAK42766"/>
        <property type="gene ID" value="SSO2645"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2645"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2728"/>
    <dbReference type="eggNOG" id="arCOG08331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122703_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VH8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032603">
        <property type="entry name" value="DUF4898"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16239">
        <property type="entry name" value="DUF4898"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="190" mass="21739" checksum="094B49B59378222D" modified="2001-10-01" version="1">MIFKTFYCKNIVIIKLNSVLRYNVCIDYTKCKMHINFKNSKAELNIKVVIMLANSADIDNLEDFIMTVARSRDIFNLKSYSLKIISDYSKFFNIILPKDVSDILIVLPISRDDLGNTIKNELTKIRTSCSILVLYSSKLQKDRMAVGFRAIVNKTISGEANMNKNKVISKRLEPLGLKTHLNLYSLGITF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VI2</accession>
    <name>Q97VI2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NUMOD4 domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2641</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90438">
        <property type="entry name" value="C90438"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988548.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VI2"/>
    <dbReference type="STRING" id="273057.SSO2641"/>
    <dbReference type="PaxDb" id="273057-SSO2641"/>
    <dbReference type="EnsemblBacteria" id="AAK42762">
        <property type="protein sequence ID" value="AAK42762"/>
        <property type="gene ID" value="SSO2641"/>
    </dbReference>
    <dbReference type="GeneID" id="1452681"/>
    <dbReference type="KEGG" id="sso:SSO2641"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2724"/>
    <dbReference type="eggNOG" id="arCOG07327">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727271_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VI2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="17956" checksum="2161C98C69CD3313" modified="2001-10-01" version="1">MGSVKKLKGYEGHVKIDENGEIEESSNIENLSKLVELIKFNLKKGNEEAKQLGFNKLNGFAMFGSNKSLTFMKGLAIVVDNDKADWQDLFTYYTYNKTFIITGGVLVLLSILLFYYGLLTPMFNFMAPEPRIYIPTILLLIGVIFLILSKSTFSYRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VI6</accession>
    <name>Q97VI6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Carbon monoxide dehydrogenase, small chain. Amino-end (CutC-2)</fullName>
            <ecNumber evidence="7">1.2.7.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cutC-2</name>
        <name evidence="7" type="ordered locus">SSO2637</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.4" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90437">
        <property type="entry name" value="G90437"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988545.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VI6"/>
    <dbReference type="FunCoup" id="Q97VI6">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2637"/>
    <dbReference type="PaxDb" id="273057-SSO2637"/>
    <dbReference type="EnsemblBacteria" id="AAK42758">
        <property type="protein sequence ID" value="AAK42758"/>
        <property type="gene ID" value="SSO2637"/>
    </dbReference>
    <dbReference type="GeneID" id="1452677"/>
    <dbReference type="KEGG" id="sso:SSO2637"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2721"/>
    <dbReference type="eggNOG" id="arCOG01925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052511_3_1_2"/>
    <dbReference type="InParanoid" id="Q97VI6"/>
    <dbReference type="PhylomeDB" id="Q97VI6"/>
    <dbReference type="BioCyc" id="MetaCyc:MONOMER-18073"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:anaerobic carbon-monoxide dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.120:FF:000003">
        <property type="entry name" value="Carbon monoxide dehydrogenase, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000020">
        <property type="entry name" value="Xanthine dehydrogenase iron-sulfur subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.120">
        <property type="entry name" value="[2Fe-2S]-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002888">
        <property type="entry name" value="2Fe-2S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036884">
        <property type="entry name" value="2Fe-2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051452">
        <property type="entry name" value="Diverse_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053590">
        <property type="entry name" value="Glyceraldehyde_DH_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041020">
        <property type="entry name" value="glyceraldDH_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44379">
        <property type="entry name" value="OXIDOREDUCTASE WITH IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44379:SF5">
        <property type="entry name" value="OXIDOREDUCTASE WITH IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01799">
        <property type="entry name" value="Fer2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47741">
        <property type="entry name" value="CO dehydrogenase ISP C-domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="6">
        <location>
            <begin position="10"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="163" mass="17933" checksum="FDCD218ECFD0F5F6" modified="2001-10-01" version="1">MLMVNQGEKIKIKVKVNGVLYERYVSPRILLVDFLREELGLTGTKIGCDTTTCGACTVLLNGKSVKSCTLFAVQADGAEITTIEGLSVDSKLHPIQEAFKENFALQCGFCTPGMIMQAYFLLKENPNPSEEEVRDGLHGNICRCTGYQNIVKAVLDASRRLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VM1</accession>
    <name>Q97VM1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2601</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90433">
        <property type="entry name" value="D90433"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989316.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VM1"/>
    <dbReference type="STRING" id="273057.SSO2601"/>
    <dbReference type="PaxDb" id="273057-SSO2601"/>
    <dbReference type="EnsemblBacteria" id="AAK42723">
        <property type="protein sequence ID" value="AAK42723"/>
        <property type="gene ID" value="SSO2601"/>
    </dbReference>
    <dbReference type="GeneID" id="1454050"/>
    <dbReference type="KEGG" id="sso:SSO2601"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2682"/>
    <dbReference type="eggNOG" id="arCOG08529">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_603599_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VM1"/>
    <dbReference type="PhylomeDB" id="Q97VM1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018646">
        <property type="entry name" value="12TM_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09847">
        <property type="entry name" value="12TM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="64"/>
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            <begin position="183"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="371"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="453" mass="51365" checksum="E074B6979C58438C" modified="2001-10-01" version="1">MSNVKIHDVVYKQIIYLMYTSRSFGLSPKPDAISKIRRNAVTIKISNIIAYTIATIVSASISLINKDAPFSFIFLDLIILANIFTTGLNVIFFVTNYDLKTFLLSLPLSERDVNRAVFRGIFEFFYYGFLASIVIAPISTYMITSSVLQALMAELEIIFFFSLSFALVMLLGKRIRLGITSALFRIGTSLIWIVFIMLPYGLTFKYVTIPTYILPIFPFGFLNIEGLLISLLYTGLSVFFAYKQSLKFLSFRLNSQYSTKYSIKLRSPLITYLYKDIRGLLRVPQASFLLTIPVFALIFSFFAPVYAIFYTIFMITTSSIMLILLEASGMQLLLSLPAGLRSSYISKLLIILIIYLIDVLIFSFFNRASLSLIMLPSTITSVELSLFISYNNVIKGKGMRLADPLSFIIREIEINSIIGIASILTFFANIYYSLLFSVLSLIMINIVVYKKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="137" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VU5</accession>
    <name>Q97VU5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="17">3-hydroxyacyl-CoA dehydrogenase/enoyl CoA hydratase</fullName>
            <ecNumber evidence="17">1.1.1.157</ecNumber>
            <ecNumber evidence="17">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">SSO2514</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.157" evidence="17"/>
    <dbReference type="EC" id="4.2.1.17" evidence="17"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VU5"/>
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    </dbReference>
    <dbReference type="STRING" id="273057.SSO2514"/>
    <dbReference type="PaxDb" id="273057-SSO2514"/>
    <dbReference type="EnsemblBacteria" id="AAK42645">
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        <property type="gene ID" value="SSO2514"/>
    </dbReference>
    <dbReference type="GeneID" id="1453972"/>
    <dbReference type="KEGG" id="sso:SSO2514"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2591"/>
    <dbReference type="eggNOG" id="arCOG00249">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_010448_2_1_2"/>
    <dbReference type="InParanoid" id="Q97VU5"/>
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    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0008691">
        <property type="term" value="F:3-hydroxybutyryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="GO_Central"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
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    </dbReference>
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        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
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    </dbReference>
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        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
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        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23309">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23309:SF49">
        <property type="entry name" value="PEROXISOMAL BIFUNCTIONAL ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="16">
        <location>
            <begin position="8"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="15">
        <location>
            <begin position="190"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="15">
        <location>
            <begin position="311"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AAK42645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="663" mass="73039" checksum="02E45FC541DCFFF5" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VU8</accession>
    <name>Q97VU8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ChsH2 rubredoxin-like zinc ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2509</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2020" name="FEBS Open Bio" volume="10" first="221" last="228">
            <title>Crystal structure of the translation recovery factor Trf from Sulfolobussolfataricus.</title>
            <authorList>
                <person name="Kaiser M."/>
                <person name="Wurm J.P."/>
                <person name="Martens B."/>
                <person name="Blasi U."/>
                <person name="Pogoryelov D."/>
                <person name="Wohnert J."/>
            </authorList>
            <dbReference type="PubMed" id="31804766"/>
            <dbReference type="DOI" id="10.1002/2211-5463.12772"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 1-119 IN COMPLEX WITH ZN(2+)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90423">
        <property type="entry name" value="C90423"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923908.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="6HTJ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.65 A"/>
        <property type="chains" value="A/B=1-119"/>
    </dbReference>
    <dbReference type="PDBsum" id="6HTJ"/>
    <dbReference type="AlphaFoldDB" id="Q97VU8"/>
    <dbReference type="SMR" id="Q97VU8"/>
    <dbReference type="STRING" id="273057.SSO2509"/>
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    <dbReference type="EnsemblBacteria" id="AAK42642">
        <property type="protein sequence ID" value="AAK42642"/>
        <property type="gene ID" value="SSO2509"/>
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    <dbReference type="GeneID" id="1453969"/>
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    <dbReference type="PATRIC" id="fig|273057.12.peg.2588"/>
    <dbReference type="eggNOG" id="arCOG01289">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_119412_1_2_2"/>
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    <dbReference type="PhylomeDB" id="Q97VU8"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="ChsH2_Znr"/>
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        <property type="entry name" value="NA-bd_OB-fold"/>
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    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
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        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="1">
        <location>
            <begin position="15"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="6HTJ"/>
        </source>
    </evidence>
    <sequence length="124" mass="14786" checksum="12EEB26C58BACE31" modified="2001-10-01" version="1">MREEEIRELYFKYFDENKLPFIQCNKCGHKFYYPRVLCPKCGSSDIEVRFSKGLGKIFAMTKVYRKDGSYVIYGIVELEEGFRMYSNIIEESQADINRKVEVIFKEINGKKYPLFKTVTHLHNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VY5</accession>
    <name>Q97VY5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2468</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAK42605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="F90418"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923873.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VY5"/>
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    <dbReference type="PaxDb" id="273057-SSO2468"/>
    <dbReference type="EnsemblBacteria" id="AAK42605">
        <property type="protein sequence ID" value="AAK42605"/>
        <property type="gene ID" value="SSO2468"/>
    </dbReference>
    <dbReference type="GeneID" id="15296738"/>
    <dbReference type="KEGG" id="sso:SSO2468"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2545"/>
    <dbReference type="eggNOG" id="arCOG00174">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036171_2_0_2"/>
    <dbReference type="InParanoid" id="Q97VY5"/>
    <dbReference type="PhylomeDB" id="Q97VY5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42744">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR42744:SF1">
        <property type="entry name" value="BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEMS INNER MEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="432"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="495"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="11"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="320"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="535" mass="60704" checksum="A887E75EF6B943A1" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VY6</accession>
    <name>Q97VY6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Biotin carboxylase a subunit of propionyl-CoA carboxylase (AccC)</fullName>
            <ecNumber evidence="8">6.3.4.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">accC</name>
        <name evidence="8" type="ordered locus">SSO2466</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="6.3.4.14" evidence="8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90418">
        <property type="entry name" value="E90418"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923872.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VY6"/>
    <dbReference type="FunCoup" id="Q97VY6">
        <property type="interactions" value="404"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2466"/>
    <dbReference type="PaxDb" id="273057-SSO2466"/>
    <dbReference type="EnsemblBacteria" id="AAK42604">
        <property type="protein sequence ID" value="AAK42604"/>
        <property type="gene ID" value="SSO2466"/>
    </dbReference>
    <dbReference type="GeneID" id="1453931"/>
    <dbReference type="KEGG" id="sso:SSO2466"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2544"/>
    <dbReference type="eggNOG" id="arCOG01590">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_3_2_2"/>
    <dbReference type="InParanoid" id="Q97VY6"/>
    <dbReference type="PhylomeDB" id="Q97VY6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45007">
        <property type="entry name" value="CARBOXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G07570)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45007:SF1">
        <property type="entry name" value="CARBOXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G07570)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="7">
        <location>
            <begin position="3"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="6">
        <location>
            <begin position="122"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="510" mass="57114" checksum="FFD70F8375218411" modified="2001-10-01" version="1">MSPFNKVLVANRGEIAIRVMKAVKEMGMKAVAVYSDADKYAPHVKYADEAYWIGPPPALESYLNIERIIDAAEKAHADAVHPGYGFLSENASFVEAVEKAGMVFIGPSASVMNRIKDKLEGKMIARKAGVPTSPGPLTPIENVDEALKIAGEIGYPIMLKAAGGGAGVGIIKVDNPSELAEAFERSKRLAYSAFGRAEIYIEKAAIKPKHIETQLIGDKYGNYVVAFERECTIQRRNQKLIEEAPSPSIKEEERKEIIEASIRFGKEINYFTLGTMEFVFSPVTREFYFLEINKRVQVEHTVTEFITGIDLVKLQIRLAAGEYLPFSQEDLKIRGHAIQFRINAEDPLNNFTPQSGYITYYKEPTGPGVRVDSGIELGSWVPPYYDPLVSKLIVYGQSRDYAIQVGLRALNDYKIGGVKTTIPLYKLILQDPDFWEGNFTTAYISEKAEYFTTKLKEEQEIQIAIAISIYNRGLMKKKKTEQKVPISTSNGKSNWKTYGIIFQSSPRVLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W12</accession>
    <name>Q97W12_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">uroporphyrinogen-III C-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cobA</name>
        <name evidence="7" type="ordered locus">SSO2435</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.107" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90415">
        <property type="entry name" value="C90415"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989387.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W12"/>
    <dbReference type="FunCoup" id="Q97W12">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2435"/>
    <dbReference type="PaxDb" id="273057-SSO2435"/>
    <dbReference type="EnsemblBacteria" id="AAK42578">
        <property type="protein sequence ID" value="AAK42578"/>
        <property type="gene ID" value="SSO2435"/>
    </dbReference>
    <dbReference type="GeneID" id="1453905"/>
    <dbReference type="GeneID" id="44128155"/>
    <dbReference type="KEGG" id="sso:SSO2435"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2515"/>
    <dbReference type="eggNOG" id="arCOG00644">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_7_0_2"/>
    <dbReference type="InParanoid" id="Q97W12"/>
    <dbReference type="PhylomeDB" id="Q97W12"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000001">
        <property type="entry name" value="Siroheme synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000018">
        <property type="entry name" value="Uroporphyrin-III C-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004790">
        <property type="entry name" value="PRK06136.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790:SF3">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT UROPORPHYRINOGEN III METHYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="6">
        <location>
            <begin position="4"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="242" mass="26830" checksum="BCAFD1DE0A1AEBC6" modified="2001-10-01" version="1">MSGKVYLVGAGPGDPELITVKGLKILQRADVVVYDRLISKELLNFCKIEAEKIYVGKNIGDYVIQDEINELLVKKAMENKVVVRLKGGDPYVLGRGEEECLYVISKGIECEVIPGITSAIAVPAYAGIPVTSRIYSASGFTIISGTKAEDKLIDEDYIPRKGTLVILMGLRKIENMVNILIKLRNEREPVAVIENGTTENQRVFTGELKDLVNIVRSNNVTSPAVIVIGEVVKFREYLWKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W29</accession>
    <name>Q97W29_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2413</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90413">
        <property type="entry name" value="B90413"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989414.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W29"/>
    <dbReference type="STRING" id="273057.SSO2413"/>
    <dbReference type="PaxDb" id="273057-SSO2413"/>
    <dbReference type="EnsemblBacteria" id="AAK42561">
        <property type="protein sequence ID" value="AAK42561"/>
        <property type="gene ID" value="SSO2413"/>
    </dbReference>
    <dbReference type="GeneID" id="1453888"/>
    <dbReference type="KEGG" id="sso:SSO2413"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2496"/>
    <dbReference type="eggNOG" id="arCOG05949">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156995_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W29"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="147" mass="17175" checksum="88B17D3545B2245F" modified="2001-10-01" version="1">MIISIPSTIRKEVNDFTFVFSVIPPIITLKNADEDVKEFLLQLSKSFRIDIACENRDKKLCYPAIFGGIFVFNHDVIIKRYEITGYLCNGKEETVKNVNQLFKRLEEGKDWCFKFNDNSILCYKTTKESKECKWIDNIGLRFLILST</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W47</accession>
    <name>Q97W47_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Iron-sulfur protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2398</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HdrC family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90410">
        <property type="entry name" value="H90410"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989442.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W47"/>
    <dbReference type="STRING" id="273057.SSO2398"/>
    <dbReference type="PaxDb" id="273057-SSO2398"/>
    <dbReference type="EnsemblBacteria" id="AAK42543">
        <property type="protein sequence ID" value="AAK42543"/>
        <property type="gene ID" value="SSO2398"/>
    </dbReference>
    <dbReference type="GeneID" id="1453870"/>
    <dbReference type="KEGG" id="sso:SSO2398"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2478"/>
    <dbReference type="eggNOG" id="arCOG07320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_374933_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W47"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051460">
        <property type="entry name" value="HdrC_iron-sulfur_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255:SF1">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="343"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="751" mass="86182" checksum="64438609F7A87ED6" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W63</accession>
    <name>Q97W63_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">SSO2374</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90408">
        <property type="entry name" value="F90408"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989466.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W63"/>
    <dbReference type="SMR" id="Q97W63"/>
    <dbReference type="FunCoup" id="Q97W63">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2374"/>
    <dbReference type="PaxDb" id="273057-SSO2374"/>
    <dbReference type="EnsemblBacteria" id="AAK42525">
        <property type="protein sequence ID" value="AAK42525"/>
        <property type="gene ID" value="SSO2374"/>
    </dbReference>
    <dbReference type="GeneID" id="1453852"/>
    <dbReference type="KEGG" id="sso:SSO2374"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2459"/>
    <dbReference type="eggNOG" id="arCOG01180">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018693_3_3_2"/>
    <dbReference type="InParanoid" id="Q97W63"/>
    <dbReference type="PhylomeDB" id="Q97W63"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05145">
        <property type="entry name" value="RIO1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051272">
        <property type="entry name" value="RIO-type_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45723">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="45"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAK42525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="254" mass="29471" checksum="C77BB2DB3B259929" modified="2001-10-01" version="1">MTELPKKTKDEKRRKDEDLFKVVDSTIDYRTYLNLIQIARRLNIKEYLGAISSGKEARIYPARTFDDTYYAVKIYYTSTAQSKRAIKKYTIGDIRFEDIKVTNTKQLINTWAKKEFKNLSRLYEAGTRVPKPILVFENVLVMEFIGENGIRAPLLKELSDEEITQGLYEDLIQQVEIMVKGAKLIHGDLSEYNVIVYDNKCYIIDVGQAVPIDYEDAITLLKRDLDNINRFFENKGINIRPTEELLIKYGVTGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WA9</accession>
    <name>Q97WA9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2326</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90402">
        <property type="entry name" value="H90402"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992500.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WA9"/>
    <dbReference type="STRING" id="273057.SSO2326"/>
    <dbReference type="PaxDb" id="273057-SSO2326"/>
    <dbReference type="EnsemblBacteria" id="AAK42479">
        <property type="protein sequence ID" value="AAK42479"/>
        <property type="gene ID" value="SSO2326"/>
    </dbReference>
    <dbReference type="GeneID" id="1453806"/>
    <dbReference type="KEGG" id="sso:SSO2326"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2412"/>
    <dbReference type="eggNOG" id="arCOG05970">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1902033_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WA9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="133" mass="15252" checksum="237FD1967BD138F5" modified="2001-10-01" version="1">MIREIYKLLLVGVISFLIIVTVISRLYIVLVPIVLFSIYLINESRIPEIKDLKSFHKYVEKVYGRDFAAIIKKRYNIIQGDLTLAYFPSSIEDNTVVIANTHLILKINSRVFVLSKYEGVDYLVDIIKGNVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WC9</accession>
    <name>Q97WC9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cobalamin biosynthesis precorrin-3 methylase, putative (CbiF)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cbiF</name>
        <name evidence="9" type="ordered locus">SSO2299</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90400">
        <property type="entry name" value="D90400"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991811.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WC9"/>
    <dbReference type="FunCoup" id="Q97WC9">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2299"/>
    <dbReference type="PaxDb" id="273057-SSO2299"/>
    <dbReference type="EnsemblBacteria" id="AAK42459">
        <property type="protein sequence ID" value="AAK42459"/>
        <property type="gene ID" value="SSO2299"/>
    </dbReference>
    <dbReference type="GeneID" id="1453786"/>
    <dbReference type="KEGG" id="sso:SSO2299"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2391"/>
    <dbReference type="eggNOG" id="arCOG00645">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_7_1_2"/>
    <dbReference type="InParanoid" id="Q97WC9"/>
    <dbReference type="PhylomeDB" id="Q97WC9"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046026">
        <property type="term" value="F:precorrin-4 C11-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11641">
        <property type="entry name" value="Precorrin-4_C11-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006362">
        <property type="entry name" value="Cbl_synth_CobM/CibF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01465">
        <property type="entry name" value="cobM_cbiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004790">
        <property type="entry name" value="PRK06136.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790:SF4">
        <property type="entry name" value="COBALT-PRECORRIN-4 C(11)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4 7 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="8">
        <location>
            <begin position="4"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="262" mass="28723" checksum="77E65F98A5940366" modified="2001-10-01" version="1">MIGKVVFIGSGPGDPELITIKAKKYIETADVIVYAGSLVNPEILKWSRKDAEVYNSSSLTLNEIVEIMVKKASEGKLVARLKSGDSSIYGALFEEMWALEAAGIPFEVIPGITAAIAAASVIPIELTVPKLSQTVIITRASLRVPMQGSIKDFAKMVKIGATMVIYTGIHIIDRVVNDLKEGGLTDDTPVIVVYRATWPEQKIIKGTLADIVSKVREAKIYRDSVIIVGLSADPQQIKNMVRSSVYDPKHNHSYRPWKVEED</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WE6</accession>
    <name>SYC_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cysteine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cysteinyl-tRNA synthetase</fullName>
            <shortName evidence="1">CysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cysS</name>
        <name type="ordered locus">SSO2280</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Cys) + L-cysteine + ATP = L-cysteinyl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90398">
        <property type="entry name" value="C90398"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991836.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WE6"/>
    <dbReference type="SMR" id="Q97WE6"/>
    <dbReference type="FunCoup" id="Q97WE6">
        <property type="interactions" value="221"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2280"/>
    <dbReference type="PaxDb" id="273057-SSO2280"/>
    <dbReference type="EnsemblBacteria" id="AAK42442">
        <property type="protein sequence ID" value="AAK42442"/>
        <property type="gene ID" value="SSO2280"/>
    </dbReference>
    <dbReference type="GeneID" id="1453769"/>
    <dbReference type="GeneID" id="44128012"/>
    <dbReference type="KEGG" id="sso:SSO2280"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2373"/>
    <dbReference type="eggNOG" id="arCOG00486">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013528_0_1_2"/>
    <dbReference type="InParanoid" id="Q97WE6"/>
    <dbReference type="PhylomeDB" id="Q97WE6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000068">
        <property type="entry name" value="Cysteine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1910">
        <property type="entry name" value="Cysteine-tRNA ligase, C-terminal anti-codon recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015273">
        <property type="entry name" value="Cys-tRNA-synt_Ia_DALR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF3">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09190">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00840">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000159546" description="Cysteine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="266"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00041"/>
        </source>
    </evidence>
    <sequence length="470" mass="55326" checksum="A81295E3FFBDB5C5" modified="2001-10-01" version="1">MDFRIRVYNSLGRKLEEFGTVEPNLVKMYVCGPTVYDYVHIGHGRTFVVFDAISRYLRLRGYTVIRVQNITDIDDKIIKKSQEIGKDWNEIVDYFTKDYLDMLSQLKVKIDIHPRVTQHIREIIDFVQRLIDKGHAYVAPSGSVYFDVDTYPNYGELSNTKKEEWNQGEEFVKEKKHSYDFALWKAWKPGEPYWESPWGKGRPGWHIECSTMSTRYLGERFDIHGGGADLIFPHHENERAQTEALIGEKWVTYWVHSAFVTIRKEKMSKSLGNIIPLNEAIKKWGPSVLRYWYLTSHYRSPIDFSEEALEQAKSALQRIKDSMAIIRDVISEGPKFYVKDDDIKVYREILNNLNNFHTAMSNDFDTSTALSYIHEIVRLVFSTLQYSRDFLGAMLAFETLKQFNEVFGVMDEEFYPTYDKMYKIIDAVVDIRNQLRQMKLYEISDKIREELLKAGVRILDSKDKSTWRFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WL6</accession>
    <name>Q97WL6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Helicase HerA central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2200</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HerA family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90389">
        <property type="entry name" value="C90389"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992093.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WL6"/>
    <dbReference type="SMR" id="Q97WL6"/>
    <dbReference type="STRING" id="273057.SSO2200"/>
    <dbReference type="PaxDb" id="273057-SSO2200"/>
    <dbReference type="EnsemblBacteria" id="AAK42370">
        <property type="protein sequence ID" value="AAK42370"/>
        <property type="gene ID" value="SSO2200"/>
    </dbReference>
    <dbReference type="GeneID" id="1453697"/>
    <dbReference type="KEGG" id="sso:SSO2200"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2296"/>
    <dbReference type="eggNOG" id="arCOG00280">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_488874_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WL6"/>
    <dbReference type="PhylomeDB" id="Q97WL6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF2">
        <property type="entry name" value="HELICASE HERA CENTRAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase HerA central" evidence="5">
        <location>
            <begin position="133"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="537" mass="60141" checksum="752F08818FED5D57" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WM9</accession>
    <name>Q97WM9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90387">
        <property type="entry name" value="F90387"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WM9"/>
    <dbReference type="PaxDb" id="273057-SSO2183"/>
    <dbReference type="EnsemblBacteria" id="AAK42357">
        <property type="protein sequence ID" value="AAK42357"/>
        <property type="gene ID" value="SSO2183"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2183"/>
    <dbReference type="eggNOG" id="arCOG08370">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2393039_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WM9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="100" mass="11542" checksum="545A35756DCB1CCE" modified="2001-10-01" version="1">MAMVIRRTFIKSTLAVSFSISYLLYLIARASFGSGKLAFVIALLQLYPYFLIEYLPTGQVISLFFQSLLLLLFAIFVLLITYILFSFYKLYKMIKTSVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WN1</accession>
    <name>Q97WN1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Peptidase related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO2181</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90387">
        <property type="entry name" value="D90387"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992117.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WN1"/>
    <dbReference type="STRING" id="273057.SSO2181"/>
    <dbReference type="PaxDb" id="273057-SSO2181"/>
    <dbReference type="EnsemblBacteria" id="AAK42355">
        <property type="protein sequence ID" value="AAK42355"/>
        <property type="gene ID" value="SSO2181"/>
    </dbReference>
    <dbReference type="GeneID" id="1453682"/>
    <dbReference type="KEGG" id="sso:SSO2181"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2277"/>
    <dbReference type="eggNOG" id="arCOG03665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006754_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WN1"/>
    <dbReference type="PhylomeDB" id="Q97WN1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000027">
        <property type="entry name" value="Peptidase S53 propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017001">
        <property type="entry name" value="Pept_S53_physarolisin-II_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF15">
        <property type="entry name" value="TRIPEPTIDYL-PEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032623">
        <property type="entry name" value="Peptidase_SSO2181_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1050"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S53" evidence="9">
        <location>
            <begin position="166"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="1077" mass="117475" checksum="A583769A39B56A92" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WS3</accession>
    <name>Q97WS3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2043</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90371">
        <property type="entry name" value="F90371"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923749.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WS3"/>
    <dbReference type="FunCoup" id="Q97WS3">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2043"/>
    <dbReference type="PaxDb" id="273057-SSO2043"/>
    <dbReference type="EnsemblBacteria" id="AAK42229">
        <property type="protein sequence ID" value="AAK42229"/>
        <property type="gene ID" value="SSO2043"/>
    </dbReference>
    <dbReference type="GeneID" id="7805885"/>
    <dbReference type="KEGG" id="sso:SSO2043"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2124"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_16_0_2"/>
    <dbReference type="InParanoid" id="Q97WS3"/>
    <dbReference type="PhylomeDB" id="Q97WS3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052962">
        <property type="entry name" value="AA_Transporter_AGT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547:SF1">
        <property type="entry name" value="ASPARTATE-PROTON SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="427"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="454"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="486"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="514"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="541"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="580"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="616" mass="67501" checksum="BD842D2634C89A40" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WY6</accession>
    <name>Q97WY6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cas12f1-like TNB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1974</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90363">
        <property type="entry name" value="F90363"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923706.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WY6"/>
    <dbReference type="SMR" id="Q97WY6"/>
    <dbReference type="STRING" id="273057.SSO1974"/>
    <dbReference type="PaxDb" id="273057-SSO1974"/>
    <dbReference type="EnsemblBacteria" id="AAK42165">
        <property type="protein sequence ID" value="AAK42165"/>
        <property type="gene ID" value="SSO1974"/>
    </dbReference>
    <dbReference type="GeneID" id="1453492"/>
    <dbReference type="KEGG" id="sso:SSO1974"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2050"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726877_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WY6"/>
    <dbReference type="PhylomeDB" id="Q97WY6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF21">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="298"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="382" mass="45152" checksum="55905F5F26C05564" modified="2001-10-01" version="1">MARRGNKAIRATVSMKIALSEPLLVLVNNYVKALRFTLFWLKENVPNPEEKGVLSKVHEALYEKLREEYNLPSKVAEDCYREALATYKGWYNNPRRGRFPRVYRPTVWLTPRASYNVDFEAMTVRIVSVGELSILGYPRNLKEYMGWKMKEARLVIRDDKAFLKVVFEKEEQKVEPKESIAVDINMAEVVVGKDDKNYVRIPTRLEEVHHWKSLAERLLKKYPRRWRENKRILYRVRSFHLKARRIMEDFARKVGKWVVDVAKRMGVNVIKLESLKNLIKSVEKLSKEFRDKLYLMQYRRLQYWISWQAKKRGMTVEFVNPSYSSVSCPKCGKRMVEVSHRWFKCSCGYENDRDVIAIMNLNGRGSLTLSTAPQMRDVNPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X09</accession>
    <name>Q97X09_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyl-hydrolase family 116 catalytic region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1948</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90360">
        <property type="entry name" value="F90360"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989082.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X09"/>
    <dbReference type="FunCoup" id="Q97X09">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1948"/>
    <dbReference type="CAZy" id="GH116">
        <property type="family name" value="Glycoside Hydrolase Family 116"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1948"/>
    <dbReference type="EnsemblBacteria" id="AAK42141">
        <property type="protein sequence ID" value="AAK42141"/>
        <property type="gene ID" value="SSO1948"/>
    </dbReference>
    <dbReference type="GeneID" id="7806406"/>
    <dbReference type="KEGG" id="sso:SSO1948"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2023"/>
    <dbReference type="eggNOG" id="arCOG03865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027051_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X09"/>
    <dbReference type="PhylomeDB" id="Q97X09"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000070">
        <property type="entry name" value="Putative bile acid beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006775">
        <property type="entry name" value="GH116_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024462">
        <property type="entry name" value="GH116_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052566">
        <property type="entry name" value="Non-lysos_glucosylceramidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654">
        <property type="entry name" value="BILE ACID BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654:SF0">
        <property type="entry name" value="NON-LYSOSOMAL GLUCOSYLCERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04685">
        <property type="entry name" value="DUF608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12215">
        <property type="entry name" value="Glyco_hydr_116N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl-hydrolase family 116 N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl-hydrolase family 116 catalytic region" evidence="1">
        <location>
            <begin position="327"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="661" mass="75725" checksum="68BFB0EDD441A0FD" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X11</accession>
    <name>Q97X11_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1028 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1945</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90360">
        <property type="entry name" value="C90360"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X11"/>
    <dbReference type="PaxDb" id="273057-SSO1945"/>
    <dbReference type="EnsemblBacteria" id="AAK42138">
        <property type="protein sequence ID" value="AAK42138"/>
        <property type="gene ID" value="SSO1945"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1945"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2018"/>
    <dbReference type="eggNOG" id="arCOG04204">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068244_1_0_2"/>
    <dbReference type="InParanoid" id="Q97X11"/>
    <dbReference type="PhylomeDB" id="Q97X11"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010430">
        <property type="entry name" value="DUF1028"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39328">
        <property type="entry name" value="BLL2871 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39328:SF1">
        <property type="entry name" value="BLL2871 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06267">
        <property type="entry name" value="DUF1028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="14437" checksum="11B5A84020B2E776" modified="2001-10-01" version="1">MTFSIVIYDPEEKAWGVGVASKFLAVGAFVPWLRPNVGAIATQALANLTYGVNGLSLLEKGYSASDTIRVLTESDPLREKRQVGIVDTKGSAAAFTGKECYSYAGHIVGNNFTVQGNILAGEEVLERVSQAQIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X24</accession>
    <name>Q97X24_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1928</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90358">
        <property type="entry name" value="E90358"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923658.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X24"/>
    <dbReference type="STRING" id="273057.SSO1928"/>
    <dbReference type="PaxDb" id="273057-SSO1928"/>
    <dbReference type="EnsemblBacteria" id="AAK42124">
        <property type="protein sequence ID" value="AAK42124"/>
        <property type="gene ID" value="SSO1928"/>
    </dbReference>
    <dbReference type="GeneID" id="1453451"/>
    <dbReference type="KEGG" id="sso:SSO1928"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2003"/>
    <dbReference type="eggNOG" id="arCOG07304">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2032961_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X24"/>
    <dbReference type="PhylomeDB" id="Q97X24"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="110" mass="12676" checksum="96AD31844488E7B9" modified="2001-10-01" version="1">MKVLIKDVDEKLYRMLKAKASIEGISVSEAINEAIKLWLINKDLDRIMVIKSKDFWDAVNEGKYALFCDGNFIGSFENEDEMIKEARKYNKCYALSKKWLIGEGELTGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X45</accession>
    <name>Q97X45_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90355">
        <property type="entry name" value="H90355"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989002.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X45"/>
    <dbReference type="PaxDb" id="273057-SSO1912"/>
    <dbReference type="DNASU" id="1453430"/>
    <dbReference type="EnsemblBacteria" id="AAK42103">
        <property type="protein sequence ID" value="AAK42103"/>
        <property type="gene ID" value="SSO1912"/>
    </dbReference>
    <dbReference type="GeneID" id="1453430"/>
    <dbReference type="KEGG" id="sso:SSO1912"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1972"/>
    <dbReference type="eggNOG" id="arCOG08427">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1607235_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X45"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="165" mass="18556" checksum="FA4F2FC2CF1CAD34" modified="2001-10-01" version="1">MRFLLLLILAIIAVISKTFMLGNYIIRVNVNQNFPLVQDVENSTIGIKFIISVKNFTIEPQTIGGLLAIKNGTNFSEIIAPYYIINSTFLIIYNGSYYANITISSVQVNDNGNFDKSLTNISLIPTYSSSLTLNTFYNNRVIVMVLVAIFSLGLYILLQNRKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X62</accession>
    <name>Q97X62_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90353">
        <property type="entry name" value="C90353"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992770.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X62"/>
    <dbReference type="STRING" id="273057.SSO1891"/>
    <dbReference type="PaxDb" id="273057-SSO1891"/>
    <dbReference type="EnsemblBacteria" id="AAK42082">
        <property type="protein sequence ID" value="AAK42082"/>
        <property type="gene ID" value="SSO1891"/>
    </dbReference>
    <dbReference type="GeneID" id="1453409"/>
    <dbReference type="KEGG" id="sso:SSO1891"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1947"/>
    <dbReference type="eggNOG" id="arCOG07264">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1412422_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X62"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="192" mass="21750" checksum="FDE5482C4BC97AAD" modified="2001-10-01" version="1">MIILWSRDENLNNFKAFHIHMDKKVLIFIVLIVIISFALIMVYPSDSLLLSPQEAETIFGGKWGVLLNDTNLKSVHTITIYHVNSANLTKQQSLEVKSIEEEVLVGDVNSTNVELTIVVVKFSSNNSWSEILGNNTNYYKYNGYYVTYSATSFTYPKTIIMAYNGSTLIEITLDGYQASFTQVKEILSDLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X77</accession>
    <name>Q97X77_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1059 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO9115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90351">
        <property type="entry name" value="B90351"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X77"/>
    <dbReference type="STRING" id="273057.SSO9115"/>
    <dbReference type="PaxDb" id="273057-SSO9115"/>
    <dbReference type="EnsemblBacteria" id="AAK42065">
        <property type="protein sequence ID" value="AAK42065"/>
        <property type="gene ID" value="SSO9115"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO9115"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1928"/>
    <dbReference type="eggNOG" id="arCOG05278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200908_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X77"/>
    <dbReference type="PhylomeDB" id="Q97X77"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009409">
        <property type="entry name" value="DUF1059"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06348">
        <property type="entry name" value="DUF1059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="70" mass="7830" checksum="D2CF9B4076B31463" modified="2001-10-01" version="1">MIKMVFGIGRKKKFAFSCGSVGMDCGFEVKGASSEEEVLEILKIHAKDVHNMTEISDDIKNKIKQNIKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XB2</accession>
    <name>Q97XB2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1833</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90346">
        <property type="entry name" value="F90346"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XB2"/>
    <dbReference type="STRING" id="273057.SSO1833"/>
    <dbReference type="PaxDb" id="273057-SSO1833"/>
    <dbReference type="EnsemblBacteria" id="AAK42029">
        <property type="protein sequence ID" value="AAK42029"/>
        <property type="gene ID" value="SSO1833"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1833"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1893"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113529_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XB2"/>
    <dbReference type="PhylomeDB" id="Q97XB2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025471">
        <property type="entry name" value="DUF4322"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14210">
        <property type="entry name" value="DUF4322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4322" evidence="1">
        <location>
            <begin position="14"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="232" mass="26230" checksum="00878E5D195D4E2B" modified="2001-10-01" version="1">MRKPVLSRGEDKVITPGLPHQNNLQQVGYKLLSMLSFKGRKAEEVSRVLVFACLWNDSVESKSKGYNVSPQTVRNYVEEQGTEVIEKLLESMRRISMEILKGVKEVDISIDWTTKTWYGKPVEGLGSSAKGNSWNYATAITKYQNMVLLLAFVPQVNGMSKDEIVKFLMEQIVGMGLKVGLVTLDAGFYTVEVLKFISQFKFVIGVPVGDVKIYEEFDGEYTTNSKRHKKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XC8</accession>
    <name>Q97XC8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO1816</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90344">
        <property type="entry name" value="F90344"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992886.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XC8"/>
    <dbReference type="STRING" id="273057.SSO1816"/>
    <dbReference type="PaxDb" id="273057-SSO1816"/>
    <dbReference type="EnsemblBacteria" id="AAK42013">
        <property type="protein sequence ID" value="AAK42013"/>
        <property type="gene ID" value="SSO1816"/>
    </dbReference>
    <dbReference type="GeneID" id="1454792"/>
    <dbReference type="KEGG" id="sso:SSO1816"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1875"/>
    <dbReference type="eggNOG" id="arCOG03696">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022113_1_0_2"/>
    <dbReference type="InParanoid" id="Q97XC8"/>
    <dbReference type="PhylomeDB" id="Q97XC8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051684">
        <property type="entry name" value="Electron_Trans/Redox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30176">
        <property type="entry name" value="FERREDOXIN-TYPE PROTEIN NAPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30176:SF3">
        <property type="entry name" value="FERREDOXIN-TYPE PROTEIN NAPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="393"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="428"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="476" mass="55175" checksum="AB8FD8D533CCEFF8" modified="2001-10-01" version="1">MNIKVTVFHYILDRSYIFLLFIIFISLIFPIISAFISLIFVGLLFQGLYLRRQSSTNSPYIVLEILSMLSLIYAAQFFTHLLKATDIVFLSYLIIISLSLYRLKRIIKKKTNYLQNSKVAFTLLLLAFLLNWFISGFLDLTQGNFSVFGQFGYFSYPFLAIMNFISAFASITASPWFMIDMGIWLGVIGIFRIIEYNKLENKIRYLLMMFAYAFYSIYLPSFSPLQSEVQYIPYMWFNGLGTYGPVRSSYLLDGIIGTFTVTAILSFMFGSRQICSVTCTAPYMLQNTFLNSMKKYNRSSKVGRKTLTSRMSSWYKWVMSITWISLIILAVLSFLKFSILGNDPTMFYTSLYFNVIWYFQFMLMPFLGNYACVNSGICAWGSFNQLFGYLGFFKLKVRDLKQCLNCKTVDCANACPVGLTDMRAWFIKKGEFKSFKCVGVGDCVEVCPYNNITFYDVRSWIKEKLYPIQFKHRGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XF6</accession>
    <name>Q97XF6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1785</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90340">
        <property type="entry name" value="C90340"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923585.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XF6"/>
    <dbReference type="STRING" id="273057.SSO1785"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1785"/>
    <dbReference type="EnsemblBacteria" id="AAK41978">
        <property type="protein sequence ID" value="AAK41978"/>
        <property type="gene ID" value="SSO1785"/>
    </dbReference>
    <dbReference type="GeneID" id="1454757"/>
    <dbReference type="KEGG" id="sso:SSO1785"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1837"/>
    <dbReference type="eggNOG" id="arCOG05431">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_782162_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XF6"/>
    <dbReference type="PhylomeDB" id="Q97XF6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="206"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="350" mass="40522" checksum="6BF39D87EA4853F1" modified="2001-10-01" version="1">MDIVLRILWTGGVTRVALEEARNIPGSKLIVYRRARTYYDLSNVNLQVLFEGKSKAIYRFITSIYAGHRGDEATVDLDRIIKARDLIKGPALFHDQFSGLTGYLRWRKYKEDYGVYIHETSLDSRSVKWFLPRYLEKIVLSHSRVIITNSRWNAEILSSHGFKAEVVYPGCYPKERINLDRERIVLAVSLWDEGRRPEIYGEIAKRIKGKLIMAGSWARKDTLEEFKRKYGDVIVTGPLKEEELQNLYNRASVLIRFGFNEKGPGMGVLEAMSYGLPIIVNDGLGSKELIKDNGYVVKDWGEAVDRINEILEDEKLRKEMSIRSWEIAKELSWKNHAMRIKELMEEKIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XJ4</accession>
    <name>Q97XJ4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Terminal quinol oxidase, subunit II (DoxA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">doxA</name>
        <name evidence="3" type="ordered locus">SSO1741</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90335">
        <property type="entry name" value="C90335"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XJ4"/>
    <dbReference type="STRING" id="273057.SSO1741"/>
    <dbReference type="PaxDb" id="273057-SSO1741"/>
    <dbReference type="EnsemblBacteria" id="AAK41938">
        <property type="protein sequence ID" value="AAK41938"/>
        <property type="gene ID" value="SSO1741"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1741"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1786"/>
    <dbReference type="eggNOG" id="arCOG05342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1599036_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XJ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011636">
        <property type="entry name" value="DoxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053673">
        <property type="entry name" value="TQO_small_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041178">
        <property type="entry name" value="TQO_small_DoxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07680">
        <property type="entry name" value="DoxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Thiosulphate:quinone oxidoreductase small subunit DoxA" evidence="2">
        <location>
            <begin position="29"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="171" mass="18822" checksum="E6132DEFA40D93D5" modified="2001-10-01" version="1">MIGMDKTIVIGIIFAIITIGWILGTGQWAYGNVVGPLFNNSKLPKLEITYISASPENSSTLLILNITDVDGPDAYPASAPLMEIYNSSWHIYLNSSQISNYTVKVIQAPWNNNKKDYVNWYSGFVVILGSEAQFQLLIPIHLSPGTYYVKLYTPAIGPSSEALAKFTVVNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XM6</accession>
    <name>Q97XM6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamine amidotransferase type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1686</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90330">
        <property type="entry name" value="B90330"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923566.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XM6"/>
    <dbReference type="PaxDb" id="273057-SSO1686"/>
    <dbReference type="EnsemblBacteria" id="AAK41897">
        <property type="protein sequence ID" value="AAK41897"/>
        <property type="gene ID" value="SSO1686"/>
    </dbReference>
    <dbReference type="GeneID" id="1454676"/>
    <dbReference type="KEGG" id="sso:SSO1686"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1737"/>
    <dbReference type="eggNOG" id="arCOG03639">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086262_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XM6"/>
    <dbReference type="PhylomeDB" id="Q97XM6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42824">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42824:SF1">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YAFJ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13230">
        <property type="entry name" value="GATase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="231" mass="26604" checksum="8C93D36BB86C32F3" modified="2001-10-01" version="1">MCRMLAYYGRDKDYLKKLANCLVRASQKDPLSDDIHGDGWGVAALTTNKLVYYRSHLPIFEDKDNLESIIDSLDGEVKVIIHARQASDKNLVSVYYSHPYLESNHERLLFLAHNGSVDKYKLGEMIGIDPTLMVDSELVAKYLSIYGIKEVDKLRDVTQSALNLLLLEINRKDRSSHLFYYNYYRKDKIRRKEDYYKLYLHNGAVYSSSLAYTGCEKGEEVELGKLLVLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XU1</accession>
    <name>Q97XU1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90321">
        <property type="entry name" value="G90321"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923518.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XU1"/>
    <dbReference type="STRING" id="273057.SSO1615"/>
    <dbReference type="PaxDb" id="273057-SSO1615"/>
    <dbReference type="EnsemblBacteria" id="AAK41830">
        <property type="protein sequence ID" value="AAK41830"/>
        <property type="gene ID" value="SSO1615"/>
    </dbReference>
    <dbReference type="GeneID" id="1454609"/>
    <dbReference type="KEGG" id="sso:SSO1615"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1657"/>
    <dbReference type="eggNOG" id="arCOG07850">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_532805_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XU1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="396"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="442"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="511" mass="58500" checksum="73A60DB3C5ED7EBC" modified="2001-10-01" version="1">MIRFIGNNRRNLITALILYLLTLVPIYVFLFSNGYLNFYGPNLPLFSGKEVEHLLAASIYNPEYGFSVNDGIYFLYLLIFTSLGIYNIIILYKIFLIITFSITFLLLYTMINKAINIFKISRYKSLSLIIGVLFFLLNPLYLEYVAFNFYNLIWGVIIFSYILIKLIDIVVNDKNVTKLDILKSGIALGFSGLMDPRLYIWGSYILILTFIIVIIIKPRLILKLIKYLSLTYISSLPIIAYIYYLFVYPSLITQASTINAITVPAYRPDTYSYIATWSENSLLFNVFAFINSEWTSIVYAPPSILFYPRTQWWFFPHFGNNSLILLPPGIITYIWLASLYIFFIVAALALFDRKRTLASSLLFIPMLLIISLSAGTNVPIKEFVYLLEIDPSKLPIVGGIFATTFAIPYYAEVMALPFASYLSILGTNYLLNRINKEILKKIAVSIIIVLAVFASWQYFNGTLYPSEVTGSFPGNSISLEGYYYPLNPPPQWVHVMNLLSTSRESVRNVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y12</accession>
    <name>Q97Y12_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable succinyl-diaminopimelate desuccinylase</fullName>
            <ecNumber evidence="5">3.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">dapE</name>
        <name evidence="13" type="ordered locus">SSO1538</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-succinyl-(2S,6S)-2,6-diaminopimelate + H2O = (2S,6S)-2,6-diaminopimelate + succinate</text>
            <dbReference type="Rhea" id="RHEA:22608"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:58087"/>
            <dbReference type="EC" id="3.5.1.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.18" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90312">
        <property type="entry name" value="H90312"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992918.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y12"/>
    <dbReference type="FunCoup" id="Q97Y12">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1538"/>
    <dbReference type="PaxDb" id="273057-SSO1538"/>
    <dbReference type="EnsemblBacteria" id="AAK41759">
        <property type="protein sequence ID" value="AAK41759"/>
        <property type="gene ID" value="SSO1538"/>
    </dbReference>
    <dbReference type="GeneID" id="1454538"/>
    <dbReference type="KEGG" id="sso:SSO1538"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1576"/>
    <dbReference type="eggNOG" id="arCOG01107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_2_3_2"/>
    <dbReference type="InParanoid" id="Q97Y12"/>
    <dbReference type="PhylomeDB" id="Q97Y12"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00021"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008777">
        <property type="term" value="F:acetylornithine deacetylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009014">
        <property type="term" value="F:succinyl-diaminopimelate desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006592">
        <property type="term" value="P:ornithine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08011">
        <property type="entry name" value="M20_ArgE_DapE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010182">
        <property type="entry name" value="ArgE/DapE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01910">
        <property type="entry name" value="DapE-ArgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006398">
        <property type="entry name" value="PRK08651.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF32">
        <property type="entry name" value="ARGE_DAPE-RELATED DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0170">Cobalt</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="12">
        <location>
            <begin position="197"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAK41759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="410" mass="46116" checksum="6A6FC3FE4018317C" modified="2001-10-01" version="1">MSKIDEIIEELRGEIVDFLKELIRIPTENPPGLNYEKIVNVIKNKLDEFGYKTQVIEPTDEELKSLIRFGYGKRPNLVGYLGNGNNRIAFNAHYDVVPAGEGWSVDPYKGIEKDGKIYGRGASDMKSGIVAQIYAIEMLRRAKLLPSNIQVIQTIVPDEETVGNRNAGTYYLVQKGIFKNVNYVIFTEPTGPDNVCNGHRGAIWAIVRVYGKKSHGGFPQLGIDAVKATSLMIEELYKAIPDIVSKYEISPESGKKPTILVGVVKCGTWVNTVADYCEFSIVRRLIPEENLEEVRHSIIRVLDNISKSTGVRYEYDEFYAVNTMRCDDERLISALREKIREVRGVEARTVLSAGTFDIRFTINEGIKSINYGPGRIELAHANDEYIYVKDLLDSIKVLGLLVLELSNKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y18</accession>
    <name>Q97Y18_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alkyldihydroxyacetonephosphate synthase (Precursor)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1531</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90312">
        <property type="entry name" value="B90312"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923456.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y18"/>
    <dbReference type="FunCoup" id="Q97Y18">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1531"/>
    <dbReference type="PaxDb" id="273057-SSO1531"/>
    <dbReference type="EnsemblBacteria" id="AAK41753">
        <property type="protein sequence ID" value="AAK41753"/>
        <property type="gene ID" value="SSO1531"/>
    </dbReference>
    <dbReference type="GeneID" id="1454532"/>
    <dbReference type="KEGG" id="sso:SSO1531"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1570"/>
    <dbReference type="eggNOG" id="arCOG00337">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_2_3_2"/>
    <dbReference type="InParanoid" id="Q97Y18"/>
    <dbReference type="PhylomeDB" id="Q97Y18"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF118">
        <property type="entry name" value="ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE (PRECURSOR)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="5">
        <location>
            <begin position="40"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="451" mass="51255" checksum="3FE2B428F56AA805" modified="2001-10-01" version="1">MQEFINEIQRNFSNCVVEDLDKYKRDWTPLLILEEHLGKNLGKPLLVLSPSTTEEVSNIVKLANKYYVCIVPYGGGSSVVGGSYHNNCVILDLSKLNRIIEFNEEDLSVTVEVGIKIKDLEEWLNKRGYTLDYHPQSFYLATIGGAIAHKGSGSHSSSNIENLILWLEVILPNGKAVKVGSSIRTSMSPDLVRLFIGSEGTLGVITKAKLRIFPLAPYHTDLSFLFPNFHNAIKFTREYVLRTSPPHRIVVHDKESSEMMLNIPYNIALIRVRGYDEDLVNVNEKIIRQLAMRHNANEGDRSLIRSWREVFARKYEEQMTKIISQGLWNDTLDLGATWSAMPKLYDKLKESLNSMKGIKNVLSRVTHVYTNGASLYNVVVMEQDLELLKRVWETAARVIMQLGGTISHHHGVGILKKNWVKEELGEQYDLLLLIKRMIDSNNIMNPGKVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y30</accession>
    <name>Q97Y30_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Purine nucleoside phosporylase (DeoD)</fullName>
            <ecNumber evidence="2">2.4.2.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">deoD</name>
        <name evidence="2" type="ordered locus">SSO1519</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.2.1" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90310">
        <property type="entry name" value="F90310"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y30"/>
    <dbReference type="SMR" id="Q97Y30"/>
    <dbReference type="STRING" id="273057.SSO1519"/>
    <dbReference type="PaxDb" id="273057-SSO1519"/>
    <dbReference type="EnsemblBacteria" id="AAK41741">
        <property type="protein sequence ID" value="AAK41741"/>
        <property type="gene ID" value="SSO1519"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1519"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1559"/>
    <dbReference type="eggNOG" id="arCOG01325">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1025341_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y30"/>
    <dbReference type="PhylomeDB" id="Q97Y30"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691:SF11">
        <property type="entry name" value="FI09636P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691">
        <property type="entry name" value="URIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="1">
        <location>
            <begin position="80"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="274" mass="31374" checksum="70859FD914F44FD0" modified="2001-10-01" version="1">MPFLENGSMVYGDFIRNQEVRKRITKEELGIEEDEIPERVVVTPMPFNTQFPKNFEDTLTNLGIKVNRLKVEDQILRQFGGNLLLEKDGNRGFIAFIGRGLIDFTERIRILATVSRIKDILFIGTAGSLSNEILIGDLNIPKYAIPFENVSDFYADPTIAIPQADEKLLNEVYEYAEETGVKTHSTLHATLLFPYSETTEFLNYLLNIGVSTIDMEVSAFYKMSRFYGKRAVAVLRISDMPLIELHKQEELIKARREIAVNAVFRITLRFLKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y34</accession>
    <name>Q97Y34_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1516</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90310">
        <property type="entry name" value="B90310"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923443.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y34"/>
    <dbReference type="PaxDb" id="273057-SSO1516"/>
    <dbReference type="EnsemblBacteria" id="AAK41737">
        <property type="protein sequence ID" value="AAK41737"/>
        <property type="gene ID" value="SSO1516"/>
    </dbReference>
    <dbReference type="GeneID" id="1454516"/>
    <dbReference type="KEGG" id="sso:SSO1516"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1555"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077283_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y34"/>
    <dbReference type="PhylomeDB" id="Q97Y34"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="354" mass="40255" checksum="39E14937336462DC" modified="2001-10-01" version="1">MTKELTREEYYKALEKAVNEVILSMTGTRKDVAKRLVLGAVVGRNATEIAQEAEMDYETVLNNLDKAAQAKLIEVVKKLVGDHPVLLIVDDTHDHKLYARAMPVSRNGAQIFYCRAHKRFESAIQLLVIGVKDLVNNQIYVIHIIAYIPRKVEEELKRRGEEVKFKTKIDALLEFLSSLSGLNVKSKVFDSWYVNSRTLQGNTVGELKANARVVEGGRSVPVGEFPQGEYLVEYLGTPIKLLVIDDYKGYGRRYFFSTDLNDTAEDIITTWENRWDIEVLIRELKALGLEGGSFLTWVRNSGFVALKALSLLVVQYFKYSTGLRLGAKRLARLIKSIYHEVGGIKKLFKRRRKP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-02" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y45</accession>
    <name>MSRA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptide methionine sulfoxide reductase MsrA</fullName>
            <shortName evidence="1">Protein-methionine-S-oxide reductase</shortName>
            <ecNumber evidence="1">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Peptide-methionine (S)-S-oxide reductase</fullName>
            <shortName evidence="1">Peptide Met(O) reductase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">msrA</name>
        <name type="synonym">msr</name>
        <name type="ordered locus">SSO1503</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(S)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[thioredoxin]-disulfide + L-methionine + H2O = L-methionine (S)-S-oxide + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90308">
        <property type="entry name" value="G90308"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923436.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y45"/>
    <dbReference type="SMR" id="Q97Y45"/>
    <dbReference type="FunCoup" id="Q97Y45">
        <property type="interactions" value="237"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1503"/>
    <dbReference type="PaxDb" id="273057-SSO1503"/>
    <dbReference type="EnsemblBacteria" id="AAK41726">
        <property type="protein sequence ID" value="AAK41726"/>
        <property type="gene ID" value="SSO1503"/>
    </dbReference>
    <dbReference type="GeneID" id="1454505"/>
    <dbReference type="GeneID" id="27427855"/>
    <dbReference type="KEGG" id="sso:SSO1503"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1544"/>
    <dbReference type="eggNOG" id="arCOG02816">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031040_10_0_2"/>
    <dbReference type="InParanoid" id="Q97Y45"/>
    <dbReference type="PhylomeDB" id="Q97Y45"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033744">
        <property type="term" value="F:L-methionine:thioredoxin-disulfide S-oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774:SF1">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE MSRA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000138624" description="Peptide methionine sulfoxide reductase MsrA">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01401"/>
        </source>
    </evidence>
    <sequence length="177" mass="20570" checksum="02EEF1AC5CA458B6" modified="2001-10-01" version="1">MEVATLGGGCFWCTEAVYKRVKGVISVKPGYSGGHVPNPTYEDVCTDTTGHAEVVQITFDSSIISYREILEIFFEIHDPTTLNRQGNDVGTQYRSIILYHNEEQRKIAEEMIREVEKRIGKKVVTELKPFEVFYEAEDYHHDFYDKHKYNPYCRLVISPKVKKFMKLFPDKVKIHEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y54</accession>
    <name>Q97Y54_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1491</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90307">
        <property type="entry name" value="F90307"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991657.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y54"/>
    <dbReference type="PaxDb" id="273057-SSO1491"/>
    <dbReference type="EnsemblBacteria" id="AAK41717">
        <property type="protein sequence ID" value="AAK41717"/>
        <property type="gene ID" value="SSO1491"/>
    </dbReference>
    <dbReference type="GeneID" id="1454496"/>
    <dbReference type="KEGG" id="sso:SSO1491"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1531"/>
    <dbReference type="eggNOG" id="arCOG07301">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_736971_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y54"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="371" mass="43259" checksum="B3E0ADA25A71AA4E" modified="2001-10-01" version="1">MSMIVYTTVIDGEINVKNQQKFFSNLATAVNVISQSFDVEPLKSLHEKYSDVTKGLDEVERKDGAFYASYLFHKVFDDYFNNGRLKALLEEVKESNIEKKVKEVIKRSEEEANDEVDDNLPVVWSFKTPDIFSVFKKIDSVSGKEFETEYLGIKVYLTIRPYSDELGYYAPFLFFTKNKPRLGVKFGDISVDPYEIRVLQLNEERENFVLTFLEKILGNLTLKSKTRLEIREVSQFSNTEYLLIALRYTHWLLRRTKLTLEKAVNYFPFLLASVDKPVRFVQNIKDLYHRIEKDGVDLDTIRKEIENLGWYNITLPSKVNIQQVKGINKIDELLKIGMKLGNPIMMIVYVGMSIIYVYKVNGYDFDKVLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y57</accession>
    <name>Q97Y57_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1058</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90307">
        <property type="entry name" value="C90307"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y57"/>
    <dbReference type="STRING" id="273057.SSO1487"/>
    <dbReference type="PaxDb" id="273057-SSO1487"/>
    <dbReference type="EnsemblBacteria" id="AAK41714">
        <property type="protein sequence ID" value="AAK41714"/>
        <property type="gene ID" value="SSO1487"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1487"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1525"/>
    <dbReference type="eggNOG" id="arCOG02754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062982_1_2_2"/>
    <dbReference type="InParanoid" id="Q97Y57"/>
    <dbReference type="PhylomeDB" id="Q97Y57"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053172">
        <property type="entry name" value="Tn903_transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053520">
        <property type="entry name" value="Transposase_Tn903"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033579">
        <property type="entry name" value="transpos_IS5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631:SF3">
        <property type="entry name" value="ISSOD12 TRANSPOSASE TNPA_ISSOD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="108"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="267" mass="31380" checksum="5707DD64AF5305BB" modified="2001-10-01" version="1">MEKSKYKRDWSKYDENVITRYKLMFPFYVFEHWWDLLAEENRNARTKYKAPKEFNEFLAFLHIFLPYRAIEGVLRALEQLKIIPTSLDYSTIWERVRNMNITFPETSDELEVIADATGISTNTGGQYIVAKWGKTRDSKFLKIEIVMDNNEFNVINAEVTSNEVESAVKTVKDLQDKGKKVKKFYGDKAYDANEVYKTGVEVVVPPKKNASTKRHLARRKAVREFRKLGYDRWKEEKGYGVRWRVESLFSAVKRTFGESVRQVLLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-01-24" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y69</accession>
    <name>Q97Y69_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90305">
        <property type="entry name" value="E90305"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y69"/>
    <dbReference type="PaxDb" id="273057-SSO1473"/>
    <dbReference type="EnsemblBacteria" id="AAK41700">
        <property type="protein sequence ID" value="AAK41700"/>
        <property type="gene ID" value="SSO1473"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1473"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1511"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y69"/>
    <dbReference type="PhylomeDB" id="Q97Y69"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="13"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="191" mass="22651" checksum="3CFF9BDCDD32CC87" modified="2001-10-01" version="1">MEVADLRSDSPSQFLLRSVREVSQYMEIDYVVADAGFLNLGVIKEMPVKTIVRGKSNLKGFKELSNVPLVEKRYEVKDKVYVAYRVLKFEGLYYYDVVYVKGKPRHFMFVTNFEGDPYELAELYRLRWQVEEGFKVRKARIRYVRKLSNKIFLFLYYTVLDSAWNLVNHLLFNFKSTCKKVLSFDSFVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YB1</accession>
    <name>Q97YB1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1421</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90299">
        <property type="entry name" value="H90299"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YB1"/>
    <dbReference type="STRING" id="273057.SSO1421"/>
    <dbReference type="PaxDb" id="273057-SSO1421"/>
    <dbReference type="EnsemblBacteria" id="AAK41655">
        <property type="protein sequence ID" value="AAK41655"/>
        <property type="gene ID" value="SSO1421"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1421"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1446"/>
    <dbReference type="eggNOG" id="arCOG07523">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1567232_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YB1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="177" mass="20721" checksum="11C885F8B9A2EABB" modified="2001-10-01" version="1">MNPMTFLFKGIECEVYKITSVKLNYRAKFTYTDYYVEYHDNFLSVSEIANKMLKIKEIGHDNGRTLEDSVRELMNVVPAQKVCKHYICGKADFVREGIPGEIKTFKEEVNPIYEEKGILQAVFYAMLYGTKMSEYVSAIYEEDLNNEDYAIIKRIDFHRIILRKLSLKYLPKVEVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YF4</accession>
    <name>Q97YF4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">One of two inversely orientated ORFs in a partial ISC1043</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1371</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90293">
        <property type="entry name" value="F90293"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YF4"/>
    <dbReference type="PaxDb" id="273057-SSO1371"/>
    <dbReference type="EnsemblBacteria" id="AAK41605">
        <property type="protein sequence ID" value="AAK41605"/>
        <property type="gene ID" value="SSO1371"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1371"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1376"/>
    <dbReference type="eggNOG" id="arCOG09862">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1943953_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YF4"/>
    <dbReference type="PhylomeDB" id="Q97YF4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="129" mass="15446" checksum="9D6A9FE94457B384" modified="2001-10-01" version="1">MYYEHVSILEKFLDYDLVVYPLPKAINFLKALVNKKLTQKDFDNFKESIEDLIKLYKESRLSKIENSEESEEERLARLRRFGRELRQDLEEAKFVKIKKEQGRKSKLTPQEKAYILIIREYLGTLRGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YK0</accession>
    <name>Q97YK0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1321</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90287">
        <property type="entry name" value="H90287"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923316.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YK0"/>
    <dbReference type="PaxDb" id="273057-SSO1321"/>
    <dbReference type="EnsemblBacteria" id="AAK41559">
        <property type="protein sequence ID" value="AAK41559"/>
        <property type="gene ID" value="SSO1321"/>
    </dbReference>
    <dbReference type="GeneID" id="1454338"/>
    <dbReference type="KEGG" id="sso:SSO1321"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1323"/>
    <dbReference type="eggNOG" id="arCOG07730">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1232811_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YK0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="203" mass="24009" checksum="4C6554BE3541AE60" modified="2001-10-01" version="1">MRLLLTIDTFRQFLPFQNEKIIVIGVMEIDHINKRKDFRFFTEWDLGNEKEMITKFYEYLKGKISEVGSNNLNFFSGKSKILERLFVVGFNILRFDIPILTQKGIEYSIGTVSELNLLWSSLVVIDYLQAMLPYNKMKIKGMSWERFSYILRTTGYKVPRIPSRGSQVKELYIKKDYESILKRNKAKLLTLYSASKIFEKEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YM4</accession>
    <name>Q97YM4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">N-acetylglucosaminyltransferase related protein (NodC-like)</fullName>
            <ecNumber evidence="7">2.4.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nodC-like</name>
        <name evidence="7" type="ordered locus">SSO1299</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99285">
        <property type="entry name" value="A99285"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YM4"/>
    <dbReference type="FunCoup" id="Q97YM4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1299"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1299"/>
    <dbReference type="EnsemblBacteria" id="AAK41536">
        <property type="protein sequence ID" value="AAK41536"/>
        <property type="gene ID" value="SSO1299"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1299"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1299"/>
    <dbReference type="eggNOG" id="arCOG01389">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_659913_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YM4"/>
    <dbReference type="PhylomeDB" id="Q97YM4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050501">
        <property type="term" value="F:hyaluronan synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085029">
        <property type="term" value="P:extracellular matrix assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030213">
        <property type="term" value="P:hyaluronan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06434">
        <property type="entry name" value="GT2_HAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22913">
        <property type="entry name" value="HYALURONAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22913:SF12">
        <property type="entry name" value="MANNURONAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13641">
        <property type="entry name" value="Glyco_tranf_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="422" mass="48331" checksum="0D16CF825FA309F6" modified="2001-10-01" version="1">MALITLEIMLYELENVLYMLEYGVFIYFPVNFAIFFLFRQFLLRHFSKSYKPYISGLSRDKVKVSAIVPEYGENLEIFEKCLRSVAKNKPDEIIVVHDDKRKEVVDISKKYGAKVISLSRRVGKRGALIIGWLNAIGDIIVQLDSDTIMEDNTINEIVKPFADPKVAGVQGRPVLFRTDGRIPYLFGQIIEYSRDIVVRALNGTLNVIDGKIAAYRRSYLLETIRHFNHETYGKRKLIAADDKALTYYANMNGYKTVYQATAVAKSAAQPTFLKFLNQQLRWARSGYLYLIKEMRSGLFFKMPGKYRFHMLTYLLAPFSFALALIDTLLVPGNPTALTWSYLAYYGFNIPIILYSLLIFIFGLYLSMKISFGILNLKLPDKISFVDLITLGILGLFVIFPMFIYAAITHYGVSEWRGSSYLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z28</accession>
    <name>Q97Z28_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoate protein ligase A, hypothetical (LplA-like2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lplA-like2</name>
        <name evidence="2" type="ordered locus">SSO1112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90264">
        <property type="entry name" value="B90264"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989745.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z28"/>
    <dbReference type="FunCoup" id="Q97Z28">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1112"/>
    <dbReference type="PaxDb" id="273057-SSO1112"/>
    <dbReference type="EnsemblBacteria" id="AAK41369">
        <property type="protein sequence ID" value="AAK41369"/>
        <property type="gene ID" value="SSO1112"/>
    </dbReference>
    <dbReference type="GeneID" id="1454148"/>
    <dbReference type="KEGG" id="sso:SSO1112"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1109"/>
    <dbReference type="eggNOG" id="arCOG01939">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022986_1_1_2"/>
    <dbReference type="InParanoid" id="Q97Z28"/>
    <dbReference type="PhylomeDB" id="Q97Z28"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033819">
        <property type="term" value="F:lipoyl(octanoyl) transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009107">
        <property type="term" value="P:lipoate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16443">
        <property type="entry name" value="LplA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050664">
        <property type="entry name" value="Octanoyltrans_LipM/LipL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679:SF2">
        <property type="entry name" value="OCTANOYL-[GCVH]:PROTEIN N-OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679">
        <property type="entry name" value="OCTANOYLTRANSFERASE LIPM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="29"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="344" mass="39109" checksum="8EC46807600FC7C9" modified="2001-10-01" version="1">MRELRFLIERNSQAHILAGEEALLLSVANGSQPILRFVIFDPPAVLVGYHQAVEQEVNIEEVKKRGWEIGRRPTGGGTIIMGPWQLGWEIYANNDLLGYTPENAIKIGAEGVIRALDKLGIKASFRPKNDVEVNGRKISGIGAFSEGNYIAVTGTILLDFDVDAMISVLRLSSEKLKDKLARDFKDRLTWVNKELKRSLDMEELIKISRDSFAEVLGIKLSDDYYNEFEKKTIQELALKYSSPEWIFNLRRPLIGDDIRYVERKLPGGLVKVQVKMVSKNLIESVLVTGDFFIEPRTAIYDLEARLKWSRVEDLENEIRTWFNSVKAIGITADDLIKIIEEVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZB6</accession>
    <name>Q97ZB6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH bat-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90252">
        <property type="entry name" value="F90252"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZB6"/>
    <dbReference type="STRING" id="273057.SSO1008"/>
    <dbReference type="PaxDb" id="273057-SSO1008"/>
    <dbReference type="EnsemblBacteria" id="AAK41277">
        <property type="protein sequence ID" value="AAK41277"/>
        <property type="gene ID" value="SSO1008"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1008"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1003"/>
    <dbReference type="eggNOG" id="arCOG02272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104910_1_0_2"/>
    <dbReference type="InParanoid" id="Q97ZB6"/>
    <dbReference type="PhylomeDB" id="Q97ZB6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH bat-type" evidence="1">
        <location>
            <begin position="152"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="209" mass="24122" checksum="79A5B86C0B8C7D5C" modified="2001-10-01" version="1">MRKQLSIGMKNLYKINFSISHQGCWTSKIKDSVVTLNVSKYNNKKVRVTIASPKLIVTDLKRSENVYEILKYRKVKGGYIIDFLEDLSTTISGYILSSSNILEYRNVVREGIEKWEVTTLAKSLVGKLSERFPIDSIVVKEIKFSDMFYNGLTEKEILVLKTAITMGYFNYPRQIKAKEIAEKLGISKQDFLYHLRKSIEKIIFSSVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZD2</accession>
    <name>Q97ZD2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha amylase</fullName>
            <ecNumber evidence="4">3.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0988</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 57 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.1" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90250">
        <property type="entry name" value="E90250"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989254.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZD2"/>
    <dbReference type="SMR" id="Q97ZD2"/>
    <dbReference type="FunCoup" id="Q97ZD2">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0988"/>
    <dbReference type="CAZy" id="GH57">
        <property type="family name" value="Glycoside Hydrolase Family 57"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO0988"/>
    <dbReference type="EnsemblBacteria" id="AAK41260">
        <property type="protein sequence ID" value="AAK41260"/>
        <property type="gene ID" value="SSO0988"/>
    </dbReference>
    <dbReference type="GeneID" id="1455227"/>
    <dbReference type="KEGG" id="sso:SSO0988"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.985"/>
    <dbReference type="eggNOG" id="arCOG03278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033691_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZD2"/>
    <dbReference type="PhylomeDB" id="Q97ZD2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10795">
        <property type="entry name" value="GH57N_MJA1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052046">
        <property type="entry name" value="GH57_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004300">
        <property type="entry name" value="Glyco_hydro_57_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36306:SF1">
        <property type="entry name" value="ALPHA-AMYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36306">
        <property type="entry name" value="ALPHA-AMYLASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03065">
        <property type="entry name" value="Glyco_hydro_57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 57 N-terminal" evidence="3">
        <location>
            <begin position="6"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="447" mass="53642" checksum="E3A45F31AC2D20EE" modified="2001-10-01" version="1">MKRVIVGFEVHQPFRIRRDFFWNPRFRQKLEDRFFDTERNKEIFERIKKNCYIPATNIILSSIERAEEEGNNVKYFFSISGTFLEQAERWGREVIELFQQLAYTHKVEFLAQTYYHSVTSLWEDKSEWKEQVKMHKDTIKSYFGQYPTTFENTELITKKDIVEEVEKMGFKMMLSEGTNRNLNGRSPNYVYKLKGHEIRMLFRNYTLSDDIAFRFSNPNWDQYPLTASKYADWISRSEGNVGLIFVDYETFGEHHREQTGILEFLKWLPIELNSKGVEMMMPKEVYNDVYDEIEIAHTTSWADIEKDEKSWLGNIMQWAYDDAVRRAEMPSRELGNEYLRVWRYFTTSDNYYYLYLGHGSPAEVHSYFNAFGSPIDAFINEFYAISTFIHEEISKLNIKNEPYIFILGDKRASIAWNEKEFMEIVMRDERFKTHLKNLRLWLGNEKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZE0</accession>
    <name>HOSA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Homocitrate synthase</fullName>
            <shortName evidence="2">HCS</shortName>
            <ecNumber evidence="1 2">2.3.3.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0977</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the aldol-type condensation of 2-oxoglutarate with acetyl-CoA to yield homocitrate. Carries out the first step of the alpha-aminoadipate (AAA) lysine biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + 2-oxoglutarate + H2O = (2R)-homocitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12929"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58884"/>
            <dbReference type="EC" id="2.3.3.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12930"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha-IPM synthase/homocitrate synthase family. Homocitrate synthase LYS20/LYS21 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.14" evidence="1 2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90249">
        <property type="entry name" value="E90249"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992429.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZE0"/>
    <dbReference type="SMR" id="Q97ZE0"/>
    <dbReference type="FunCoup" id="Q97ZE0">
        <property type="interactions" value="291"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0977"/>
    <dbReference type="PaxDb" id="273057-SSO0977"/>
    <dbReference type="EnsemblBacteria" id="AAK41252">
        <property type="protein sequence ID" value="AAK41252"/>
        <property type="gene ID" value="SSO0977"/>
    </dbReference>
    <dbReference type="GeneID" id="1455219"/>
    <dbReference type="KEGG" id="sso:SSO0977"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.977"/>
    <dbReference type="eggNOG" id="arCOG02092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_4_0_2"/>
    <dbReference type="InParanoid" id="Q97ZE0"/>
    <dbReference type="PhylomeDB" id="Q97ZE0"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00028"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004410">
        <property type="term" value="F:homocitrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07940">
        <property type="entry name" value="DRE_TIM_IPMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02222">
        <property type="entry name" value="Homocitr_synth_fung_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050073">
        <property type="entry name" value="2-IPM_HCS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011872">
        <property type="entry name" value="Homocitrate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02146">
        <property type="entry name" value="LysS_fung_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002085">
        <property type="entry name" value="PRK00915.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277:SF63">
        <property type="entry name" value="HOMOCITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277">
        <property type="entry name" value="HOMOCITRATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000140423" description="Homocitrate synthase">
        <location>
            <begin position="1"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="3">
        <location>
            <begin position="4"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O87198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01151"/>
        </source>
    </evidence>
    <sequence length="461" mass="50882" checksum="E87E9E4CA21BA56F" modified="2001-10-01" version="1">MIKVGILDSTLREGEQTPGVIFTVDQRVEIAKALSDLGVSMIEAGHPAVSPDIYEGIKRIVKLKKEGIITSEIVGHSRAVKRDIEIAAELEVDRIAIFYGVSDIHLKAKHKATREEALRVIAETISYARSHGVKVRFTAEDGSRTDFDFLVTVSRTARDAGADRVSIADTVGILYPSKTKELFSALIREVPNLEYDIHAHNDLGLAVANALAAVEGGATIVHATVNGLGERVGIVPLQQIVAAIKYHFGIEVVKLDKLQYVSSLIEKYSGIPMPPNYPITGDYAFLHKAGVHVAGVLSDPRTYEFMPPETFGRTRDYTIDKYTGKHALRDKYEKLGVKISEAEMDQILAKIKSNTTIRFYRDVDLLELAEEVTGRVLKPRPPEQIEALISVKCDSNVYTTSVTRRLSVINGVKEVMEISGDYDILVKVQAKDSNELNQIIESIRATKGVRSTLTSLVLKKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZG5</accession>
    <name>Q97ZG5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90246">
        <property type="entry name" value="C90246"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992391.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZG5"/>
    <dbReference type="STRING" id="273057.SSO0952"/>
    <dbReference type="PaxDb" id="273057-SSO0952"/>
    <dbReference type="EnsemblBacteria" id="AAK41226">
        <property type="protein sequence ID" value="AAK41226"/>
        <property type="gene ID" value="SSO0952"/>
    </dbReference>
    <dbReference type="GeneID" id="1455193"/>
    <dbReference type="KEGG" id="sso:SSO0952"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.948"/>
    <dbReference type="eggNOG" id="arCOG01150">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075478_0_1_2"/>
    <dbReference type="InParanoid" id="Q97ZG5"/>
    <dbReference type="PhylomeDB" id="Q97ZG5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07391">
        <property type="entry name" value="MPP_PF1019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004376">
        <property type="entry name" value="Pesterase_MJ0037"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024173">
        <property type="entry name" value="Pesterase_MJ0037-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00024">
        <property type="entry name" value="SbcD_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39323">
        <property type="entry name" value="BLR1149 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39323:SF1">
        <property type="entry name" value="BLR1149 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000887">
        <property type="entry name" value="Pesterase_MJ0037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="25"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="251" mass="28832" checksum="5BFDEFC7C7598D15" modified="2001-10-01" version="1">MLELAKGILIAEDLPALYVKQANSVVLSDVHIGYEEEMSRKGIYIPRIQKKRFFTITNRVLSVFNTKKLIVNGDFKHTFEKLTRQEKEELNEILKYLKDNGVEVTIVRGNHDNYISLVTERFDNVKLVDELDLGEILITHGHKVVEPRNNTTYIIGHEHPRISIRDKLGFSRKLQCFLSIPIKERENSQVIVLPAIGIYQAGNDISLIHSNYMSNLIKEHTILEKAKPYIIIEGEGIMEFPELGLIKNVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZI4</accession>
    <name>Q97ZI4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0922</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90243">
        <property type="entry name" value="E90243"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992355.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZI4"/>
    <dbReference type="STRING" id="273057.SSO0922"/>
    <dbReference type="PaxDb" id="273057-SSO0922"/>
    <dbReference type="EnsemblBacteria" id="AAK41204">
        <property type="protein sequence ID" value="AAK41204"/>
        <property type="gene ID" value="SSO0922"/>
    </dbReference>
    <dbReference type="GeneID" id="1455171"/>
    <dbReference type="KEGG" id="sso:SSO0922"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.923"/>
    <dbReference type="eggNOG" id="arCOG01840">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151087_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZI4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00298">
        <property type="entry name" value="ACD_sHsps_p23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="2">
        <location>
            <begin position="40"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="122" mass="14725" checksum="944101A92BD87F15" modified="2001-10-01" version="1">MPAYKDLYDMVKSMIEKEINRMEKEFRRIESEIKEEIEKYNVPLYTFYESEDFYYYLIDVPNVDTSTLYVKVKNDSLRISCKDKSNKEYVLNIKVPEDADIDSLSVTRVKWLVKITVKRRKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-08-14" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZJ9</accession>
    <name>RPO6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo6</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1 3">DNA-directed RNA polymerase subunit K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo6</name>
        <name type="synonym">rpoK</name>
        <name type="ordered locus">SSO6768</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2008" name="Nature" volume="451" first="851" last="854">
            <title>The X-ray crystal structure of RNA polymerase from Archaea.</title>
            <authorList>
                <person name="Hirata A."/>
                <person name="Klein B.J."/>
                <person name="Murakami K.S."/>
            </authorList>
            <dbReference type="PubMed" id="18235446"/>
            <dbReference type="DOI" id="10.1038/nature06530"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF THE RNA POLYMERASE COMPLEX</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 13-subunit RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90241">
        <property type="entry name" value="F90241"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992332.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2PMZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="K/W=1-95"/>
    </dbReference>
    <dbReference type="PDB" id="3HKZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="K/U=1-95"/>
    </dbReference>
    <dbReference type="PDBsum" id="2PMZ"/>
    <dbReference type="PDBsum" id="3HKZ"/>
    <dbReference type="AlphaFoldDB" id="Q97ZJ9"/>
    <dbReference type="SMR" id="Q97ZJ9"/>
    <dbReference type="DIP" id="DIP-60642N"/>
    <dbReference type="FunCoup" id="Q97ZJ9">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="IntAct" id="Q97ZJ9">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO6768"/>
    <dbReference type="PaxDb" id="273057-SSO6768"/>
    <dbReference type="EnsemblBacteria" id="AAK41189">
        <property type="protein sequence ID" value="AAK41189"/>
        <property type="gene ID" value="SSO6768"/>
    </dbReference>
    <dbReference type="GeneID" id="1455156"/>
    <dbReference type="KEGG" id="sso:SSO6768"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.909"/>
    <dbReference type="eggNOG" id="arCOG01268">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112527_4_0_2"/>
    <dbReference type="InParanoid" id="Q97ZJ9"/>
    <dbReference type="PhylomeDB" id="Q97ZJ9"/>
    <dbReference type="BRENDA" id="2.7.7.6">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97ZJ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00192">
        <property type="entry name" value="RNApol_arch_Rpo6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020708">
        <property type="entry name" value="DNA-dir_RNA_polK_14-18kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006111">
        <property type="entry name" value="Rpo6/Rpb6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002207">
        <property type="entry name" value="PRK01099.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002208">
        <property type="entry name" value="PRK01099.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002209">
        <property type="entry name" value="PRK01099.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227:SF5">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="RPB6/omega subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01111">
        <property type="entry name" value="RNA_POL_K_14KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000133823" description="DNA-directed RNA polymerase subunit Rpo6">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="14"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="55"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="60"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="64"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="67"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="77"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="87"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18235446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="18235446"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="4">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="5">
        <source>
            <dbReference type="PDB" id="3HKZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <sequence length="95" mass="10858" checksum="2EA09FA9B1EAF55A" modified="2001-08-14" version="1">MGLERDEILSQDLHFNEVFISLWQNRLTRYEIARVISARALQLAMGAPALIDINNLSSTDVISIAEEEFRRGVLPITIRRRLPNGKIILLSLRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZK7</accession>
    <name>Q97ZK7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90240">
        <property type="entry name" value="F90240"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992322.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZK7"/>
    <dbReference type="STRING" id="273057.SSO0900"/>
    <dbReference type="PaxDb" id="273057-SSO0900"/>
    <dbReference type="EnsemblBacteria" id="AAK41181">
        <property type="protein sequence ID" value="AAK41181"/>
        <property type="gene ID" value="SSO0900"/>
    </dbReference>
    <dbReference type="GeneID" id="1455148"/>
    <dbReference type="KEGG" id="sso:SSO0900"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.902"/>
    <dbReference type="eggNOG" id="arCOG04062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152335_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZK7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="129" mass="14871" checksum="E4E66217C3C87FDA" modified="2001-10-01" version="1">MEVLLENSKSKKGKHVVRTFLFKVDSNGSIQQIELPGKPVRPTYKVGEAKIVNIPNKGVYVYLFLLRNIYGRVMGRIIVINDGKTVLVMKYRKLKLKLIDGDDKHVVIVKRIFEQLKIPVKKININKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZM3</accession>
    <name>Q97ZM3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-NAD kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0869</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90237">
        <property type="entry name" value="F90237"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992288.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZM3"/>
    <dbReference type="STRING" id="273057.SSO0869"/>
    <dbReference type="PaxDb" id="273057-SSO0869"/>
    <dbReference type="EnsemblBacteria" id="AAK41157">
        <property type="protein sequence ID" value="AAK41157"/>
        <property type="gene ID" value="SSO0869"/>
    </dbReference>
    <dbReference type="GeneID" id="1455124"/>
    <dbReference type="KEGG" id="sso:SSO0869"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.878"/>
    <dbReference type="eggNOG" id="arCOG01350">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064691_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZM3"/>
    <dbReference type="PhylomeDB" id="Q97ZM3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039065">
        <property type="entry name" value="AcoX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011386">
        <property type="entry name" value="Put_ATP-NAD_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40697">
        <property type="entry name" value="ACETOIN CATABOLISM PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40697:SF2">
        <property type="entry name" value="ATP-NAD KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016907">
        <property type="entry name" value="Kin_ATP-NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="39789" checksum="50F434A47340EBF3" modified="2001-10-01" version="1">MLKKIGFLVNPYAGSGGRIGKKGSDELNIENPEIPMRVTRFLSKAPEDAIYLTPRLKMGEIHFTKSKLKYETIPIGEKKKTTRYDTIDSVREFVKRGVDIIVFVGGDGTARDVAEGLQGAEIPILGVPAGVKMHSGVFANTPEAAAILLTKFLHDEAKTVKEEILDIDEEAYRKGTYFVKLYHIVLTISSNNLLTPSKGEMQYSEDELGEIADYIIDNMDDNITYIMGPGSTVKYIERKLKIDTPFLGIDIIRGKKLIKENVNYFDLINLTGELKIVLTPIGKQGFLIGRGNLELGPIFLRKVKKDDLIVVSTFSKLYTINCLRIDTGDENLDKLFSGVYNVIVGYNKFYAIKTCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZP3</accession>
    <name>Q97ZP3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0806</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90231">
        <property type="entry name" value="B90231"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZP3"/>
    <dbReference type="PaxDb" id="273057-SSO0806"/>
    <dbReference type="EnsemblBacteria" id="AAK41105">
        <property type="protein sequence ID" value="AAK41105"/>
        <property type="gene ID" value="SSO0806"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0806"/>
    <dbReference type="HOGENOM" id="CLU_2313920_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZP3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="99" mass="11660" checksum="1C653707CBB7EC95" modified="2001-10-01" version="1">MQSAESGLLILSSHQPLQRRVLKIKRILSYSRDTKVSHKVVLAYFRKHYWLIANGFCPYCHNHYRNFGILSNHIMRSHTLEVAEDYFKLREKIKEGGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZR3</accession>
    <name>Q97ZR3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ATP synthase subunit F (AtpF)</fullName>
            <ecNumber evidence="4">3.6.3.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">atpF</name>
        <name evidence="4" type="ordered locus">SSO0560</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.14" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90202">
        <property type="entry name" value="F90202"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991071.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZR3"/>
    <dbReference type="SMR" id="Q97ZR3"/>
    <dbReference type="FunCoup" id="Q97ZR3">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0560"/>
    <dbReference type="PaxDb" id="273057-SSO0560"/>
    <dbReference type="DNASU" id="1454844"/>
    <dbReference type="EnsemblBacteria" id="AAK40877">
        <property type="protein sequence ID" value="AAK40877"/>
        <property type="gene ID" value="SSO0560"/>
    </dbReference>
    <dbReference type="GeneID" id="1454844"/>
    <dbReference type="KEGG" id="sso:SSO0560"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.570"/>
    <dbReference type="eggNOG" id="arCOG04102">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135754_3_1_2"/>
    <dbReference type="InParanoid" id="Q97ZR3"/>
    <dbReference type="PhylomeDB" id="Q97ZR3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="entry name" value="ATPase, V1 complex, subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="AtpF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="100" mass="11357" checksum="AF3AC4FA571E769B" modified="2001-10-01" version="1">MGKVFVVGDKYTVSLFRMVGTEGDFIDDPYNLPDIISKLKKREDIDLILITKDMYEPIRGKIDPLIADQRKPLITIIPSPFSESKPLDVRKMIMKALGFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZT5</accession>
    <name>Q97ZT5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Threonine synthase (ThrC-1)</fullName>
            <ecNumber evidence="5">4.2.3.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">thrC-1</name>
        <name evidence="5" type="ordered locus">SSO0493</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90194">
        <property type="entry name" value="F90194"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZT5"/>
    <dbReference type="FunCoup" id="Q97ZT5">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0493"/>
    <dbReference type="PaxDb" id="273057-SSO0493"/>
    <dbReference type="EnsemblBacteria" id="AAK40813">
        <property type="protein sequence ID" value="AAK40813"/>
        <property type="gene ID" value="SSO0493"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0493"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.486"/>
    <dbReference type="eggNOG" id="arCOG01434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028142_4_0_2"/>
    <dbReference type="InParanoid" id="Q97ZT5"/>
    <dbReference type="PhylomeDB" id="Q97ZT5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000103">
        <property type="entry name" value="Threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005035">
        <property type="entry name" value="PRK06450.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="4">
        <location>
            <begin position="62"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="345" mass="38155" checksum="7D23D6D3A25B1FF8" modified="2001-10-01" version="1">MISSMVKEVCMKCRKERESIYEIKCNKCGGPFEILIDFEFDKNSERGFPYSEKIFPYIKHFISLGEGRTPLIKKGNIWFKLDFLNPSGSYKDRGAVTLVSYLAEKGVKQISEDSSGNAGSAIAAYSAAAGIEAYIFVPETAKGGKLKQIESYGAHVVRVRGSREDVAKAAENSGYYYASHVLQPQFRDGIRTLAYEIVKDLDWKIPNYVFIPVSAGTLLLGVYKGFKHLLDSGVISEMPKIVAVQTEQVMPLCAKFKKISYTPPDKVTSIADALVSTRPFLLDYMVKASSECIVVSDNEIVEAWKELAKMGLLVEYSSATVFAAYKKYSVNDAVLVLTGSGLKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZW1</accession>
    <name>Q97ZW1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Peptidase M50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">SSO0464</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90191">
        <property type="entry name" value="D90191"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922981.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZW1"/>
    <dbReference type="STRING" id="273057.SSO0464"/>
    <dbReference type="PaxDb" id="273057-SSO0464"/>
    <dbReference type="EnsemblBacteria" id="AAK40787">
        <property type="protein sequence ID" value="AAK40787"/>
        <property type="gene ID" value="SSO0464"/>
    </dbReference>
    <dbReference type="GeneID" id="1455597"/>
    <dbReference type="KEGG" id="sso:SSO0464"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.458"/>
    <dbReference type="eggNOG" id="arCOG00614">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099718_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZW1"/>
    <dbReference type="PhylomeDB" id="Q97ZW1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06158">
        <property type="entry name" value="S2P-M50_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052348">
        <property type="entry name" value="Metallopeptidase_M50B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044537">
        <property type="entry name" value="Rip2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35864">
        <property type="entry name" value="ZINC METALLOPROTEASE MJ0611-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35864:SF1">
        <property type="entry name" value="ZINC METALLOPROTEASE YWHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAK40787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="22443" checksum="0FD6839E8C5FA255" modified="2001-10-01" version="1">MSWDIRYLEWRFANLNEITSFLLAALSLAVAYIRPTSILSDPIGSIIIPYIVALLAIIPHEIGHRQAARRYGCFSRFTLSFSGFWTTLILNIIGSFVGILVFFSGYTSISCGLLNRDVEGKTALAGPLTNIIVGFVGLIGASLVPFSLVGLFFFELARFNFWVAFFNLLPFWVLDGLKIFRWNMIVWAVLIIIAFALTFL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZY5</accession>
    <name>THI4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiamine thiazole synthase</fullName>
            <ecNumber evidence="1">2.4.2.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thi4</name>
        <name type="ordered locus">SSO0436</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>hydrogen sulfide + glycine + NAD(+) = ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homooctamer; tetramer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the THI4 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.59" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99188">
        <property type="entry name" value="B99188"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988737.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZY5"/>
    <dbReference type="SMR" id="Q97ZY5"/>
    <dbReference type="FunCoup" id="Q97ZY5">
        <property type="interactions" value="148"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0436"/>
    <dbReference type="PaxDb" id="273057-SSO0436"/>
    <dbReference type="EnsemblBacteria" id="AAK40761">
        <property type="protein sequence ID" value="AAK40761"/>
        <property type="gene ID" value="SSO0436"/>
    </dbReference>
    <dbReference type="GeneID" id="1455571"/>
    <dbReference type="KEGG" id="sso:SSO0436"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.429"/>
    <dbReference type="eggNOG" id="arCOG00574">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053727_2_0_2"/>
    <dbReference type="InParanoid" id="Q97ZY5"/>
    <dbReference type="PhylomeDB" id="Q97ZY5"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00304">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022828">
        <property type="entry name" value="Thi4_prok"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00292">
        <property type="entry name" value="sulfide-dependent adenosine diphosphate thiazole synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422:SF3">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01946">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000300740" description="Thiamine thiazole synthase">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="60"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="160"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00304"/>
        </source>
    </evidence>
    <sequence length="267" mass="28630" checksum="47603B956AF76DA9" modified="2001-10-01" version="1">MEVKIKQVDEVKISRYIIKETMEDWYQFVESDVVIVGAGPSGLSAAYYLAKAGLKTLVFERRLSFGGGIGGGAMLFHKLIIEKPADEILREVNIRLKEVEEGVYVVDSAEFMAKLATAAIDAGAKIIHGVTVDDVIFRENPLRVAGVAVEWTATQMASLHVDPIFISAKAVVDATGHDAEVISVAARKIPELGIVIPGEKSAYSERAEELTVINTGKVAEGLYATGMAVTEVKGLPRMGPIFGAMVLSGKAVAGEITKDLLKSEIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q980H2</accession>
    <name>Q980H2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADH dehydrogenase subunit H (NuoH)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">NuoH</name>
        <name evidence="6" type="ordered locus">SSO0325</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90175">
        <property type="entry name" value="E90175"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990618.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980H2"/>
    <dbReference type="FunCoup" id="Q980H2">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0325"/>
    <dbReference type="PaxDb" id="273057-SSO0325"/>
    <dbReference type="DNASU" id="1455470"/>
    <dbReference type="EnsemblBacteria" id="AAK40660">
        <property type="protein sequence ID" value="AAK40660"/>
        <property type="gene ID" value="SSO0325"/>
    </dbReference>
    <dbReference type="GeneID" id="1455470"/>
    <dbReference type="GeneID" id="44129299"/>
    <dbReference type="KEGG" id="sso:SSO0325"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.319"/>
    <dbReference type="eggNOG" id="arCOG01546">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_0_2_2"/>
    <dbReference type="InParanoid" id="Q980H2"/>
    <dbReference type="PhylomeDB" id="Q980H2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01350">
        <property type="entry name" value="NDH1_NuoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004741">
        <property type="entry name" value="PRK06076.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432">
        <property type="entry name" value="NADH DEHYDROGENASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432:SF3">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="352" mass="39369" checksum="F82B6D033396F828" modified="2001-10-01" version="1">MSVLSALQYYFFYPSFFLTVIFPGLIFTLIFLLVTIWFERKAAAIVQLRYGPYYASKRLGGILQLFADAIKFVFSEIIIPNGVNQTLYAISPVLVLIFAFLPMALIPISTIPTQGSILSVYHGIFYDTQIHQGILVPYFIEYNLIGIIALESLYPILVIFLAWNTNNRFAIVGSIREAYLSVSYDVLIIISTLALGLEYHTLDVAKIVTSGIPGFIANPIAAFVFLVAMLIGSSRFPFEIVEAETELVIGPYTEYSGFLFVLTMAGSYVANLVYAILFVDLFLGGWLPFTGFVGAIFTVFKAAIVVFFAVFLRAVYGRYRIDQALRGSWKYFFPLALASLLLGVVVGYLWIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q980H4</accession>
    <name>Q980H4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH dehydrogenase subunit C (NuoC)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">NuoC</name>
        <name evidence="4" type="ordered locus">SSO0323</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 30 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90175">
        <property type="entry name" value="C90175"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980H4"/>
    <dbReference type="SMR" id="Q980H4"/>
    <dbReference type="FunCoup" id="Q980H4">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0323"/>
    <dbReference type="PaxDb" id="273057-SSO0323"/>
    <dbReference type="EnsemblBacteria" id="AAK40658">
        <property type="protein sequence ID" value="AAK40658"/>
        <property type="gene ID" value="SSO0323"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0323"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.317"/>
    <dbReference type="eggNOG" id="arCOG01551">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042628_6_1_2"/>
    <dbReference type="InParanoid" id="Q980H4"/>
    <dbReference type="PhylomeDB" id="Q980H4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.80">
        <property type="entry name" value="NADH:ubiquinone oxidoreductase, 30kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010218">
        <property type="entry name" value="NADH_DH_suC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037232">
        <property type="entry name" value="NADH_quin_OxRdtase_su_C/D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001268">
        <property type="entry name" value="NADH_UbQ_OxRdtase_30kDa_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01961">
        <property type="entry name" value="NuoC_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004734">
        <property type="entry name" value="PRK06074.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00329">
        <property type="entry name" value="Complex1_30kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143243">
        <property type="entry name" value="Nqo5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase 30kDa subunit" evidence="3">
        <location>
            <begin position="39"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="169" mass="19549" checksum="2949274FD17673EE" modified="2001-10-01" version="1">MGDEKMSEALKSLVNDLQTKFKCQAKIESDRRITVIVPDKKIIRDVASFLKDFGFDHVKAVTGIDYPEHEKLEVVYHISSYSNLDLAKIILALRTTVSYKDPSLPSLYSIFESAWTGERETYEMLGIRFEGHPDLRRFFLDEDFEGVYPLRKSYKIKLEGVFVDKPTTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q980I3</accession>
    <name>Q980I3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Benzene 1,2-dioxygenase system ferredoxin component, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO0312</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90174">
        <property type="entry name" value="A90174"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922935.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980I3"/>
    <dbReference type="SMR" id="Q980I3"/>
    <dbReference type="STRING" id="273057.SSO0312"/>
    <dbReference type="PaxDb" id="273057-SSO0312"/>
    <dbReference type="EnsemblBacteria" id="AAK40648">
        <property type="protein sequence ID" value="AAK40648"/>
        <property type="gene ID" value="SSO0312"/>
    </dbReference>
    <dbReference type="GeneID" id="1455458"/>
    <dbReference type="KEGG" id="sso:SSO0312"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.304"/>
    <dbReference type="eggNOG" id="arCOG02853">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_5_3_2"/>
    <dbReference type="InParanoid" id="Q980I3"/>
    <dbReference type="PhylomeDB" id="Q980I3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03467">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.102.10.10:FF:000048">
        <property type="entry name" value="Benzene 1,2-dioxygenase system ferredoxin component, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496">
        <property type="entry name" value="FERREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496:SF0">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="6">
        <location>
            <begin position="3"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="104" mass="12044" checksum="2E7B5C16769FB3A9" modified="2001-10-01" version="1">MEYIRISRTDFKTGEKRKIILPNGRELVVFYLGVDRFFVFDNKCPHLGCDLSKYGVIIKEELVCQCHFSHFSIYSGEPKKGASKKPIKVYRVQVTKDEVVISLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2026-01-28" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q980J6</accession>
    <name>CDC45_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cell division control protein 45 homolog</fullName>
            <shortName evidence="4">Cdc45</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">RecJ DNA-binding domain homolog</fullName>
            <shortName evidence="3">RecJdbh</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0295</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="EMBO Rep." volume="7" first="539" last="545">
            <title>GINS, a central nexus in the archaeal DNA replication fork.</title>
            <authorList>
                <person name="Marinsek N."/>
                <person name="Barry E.R."/>
                <person name="Makarova K.S."/>
                <person name="Dionne I."/>
                <person name="Koonin E.V."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="16485022"/>
            <dbReference type="DOI" id="10.1038/sj.embor.7400649"/>
        </citation>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2012" name="Biol. Direct" volume="7" first="7" last="7">
            <title>The CMG (CDC45/RecJ, MCM, GINS) complex is a conserved component of the DNA replication system in all archaea and eukaryotes.</title>
            <authorList>
                <person name="Makarova K.S."/>
                <person name="Koonin E.V."/>
                <person name="Kelman Z."/>
            </authorList>
            <dbReference type="PubMed" id="22329974"/>
            <dbReference type="DOI" id="10.1186/1745-6150-7-7"/>
        </citation>
        <scope>FAMILY</scope>
    </reference>
    <comment type="function">
        <text evidence="1">The CG complex (Cdc45, Gins15 and Gins23) stimulates the helicase activity of the MCM replicative DNA helicase; it copurifies throughout the cell cycle with MCM (as presumably does the CG complex) probably associated with moving replication forks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the Cdc45-GINS complex (CG complex) that includes Cdc45, Gins15 and Gins23. Interacts directly with Gins15.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-8081609">
            <id>Q980J6</id>
        </interactant>
        <interactant intactId="EBI-8081384">
            <id>Q97Z82</id>
            <label>SSO1049</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>3</experiments>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Cdc45/RecJ family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90172">
        <property type="entry name" value="D90172"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990587.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980J6"/>
    <dbReference type="IntAct" id="Q980J6">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="MINT" id="Q980J6"/>
    <dbReference type="STRING" id="273057.SSO0295"/>
    <dbReference type="PaxDb" id="273057-SSO0295"/>
    <dbReference type="EnsemblBacteria" id="AAK40635">
        <property type="protein sequence ID" value="AAK40635"/>
        <property type="gene ID" value="SSO0295"/>
    </dbReference>
    <dbReference type="GeneID" id="1455445"/>
    <dbReference type="KEGG" id="sso:SSO0295"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.291"/>
    <dbReference type="eggNOG" id="arCOG00427">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_770787_0_0_2"/>
    <dbReference type="InParanoid" id="Q980J6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017013">
        <property type="entry name" value="Arc_Cdc45"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032968">
        <property type="entry name" value="UCP032968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000463485" description="Cell division control protein 45 homolog">
        <location>
            <begin position="1"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q4JC83"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16485022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="16485022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="22329974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="359" mass="41578" checksum="CEB3950D2BF44F7F" modified="2001-10-01" version="1">MVKRNSKEIVMEVFLGEPNNQEIREVVRNVYSKDNLCLSAYYSIEPILFSFIISKNTKNSFTLSFIPEECETQIRLDANGKWIIDNKKKYFLGQNSFCSYLPITAEDVLPIVSCIITSTILDRRSLSEWELETIREMEKFDMTIEKNLKIPAYKTLPAFLSLMFSLDPYIPGISGNRENAISLIREVNASEISKLEDLNESQLNTLLFRIISTIVKENPKFTRDDIITDRFFYLDYDILELAFSLIYSFDTIGSNEILQFVLSSSYAEALISRFRQTLSKGFSINSIENKQSYYIVETSNLTSPLLAQIILLQLQKIRRDKMIVIRDNDRLFTSRFFIPSVKKEGLIKIDDRVKNLIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q980J8</accession>
    <name>Q980J8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">2-(3-amino-3-carboxypropyl)histidine synthase</fullName>
            <ecNumber evidence="3 10">2.5.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">SSO0293</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the first step of diphthamide biosynthesis, i.e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF-2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DPH1/DPH2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="3 10"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90172">
        <property type="entry name" value="B90172"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990583.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980J8"/>
    <dbReference type="FunCoup" id="Q980J8">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0293"/>
    <dbReference type="PaxDb" id="273057-SSO0293"/>
    <dbReference type="EnsemblBacteria" id="AAK40633">
        <property type="protein sequence ID" value="AAK40633"/>
        <property type="gene ID" value="SSO0293"/>
    </dbReference>
    <dbReference type="GeneID" id="1455443"/>
    <dbReference type="KEGG" id="sso:SSO0293"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.288"/>
    <dbReference type="eggNOG" id="arCOG04112">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037146_0_0_2"/>
    <dbReference type="InParanoid" id="Q980J8"/>
    <dbReference type="PhylomeDB" id="Q980J8"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11850:FF:000004">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11840:FF:000003">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035435">
        <property type="entry name" value="DPH1/DPH2_euk_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022428">
        <property type="entry name" value="Dph2_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03682">
        <property type="entry name" value="arCOG04112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF1">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004967">
        <property type="entry name" value="DPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK40633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="333" mass="38316" checksum="7A8F0902A0E6780D" modified="2001-10-01" version="1">MSYNFEEERVIKEIREKNAKRVLLQFPEGLKYFSIDIVKKLREKLPNVNFIISGEPSWGACDIAEDEAQQVNADLIIHFGHTPYTWYYPKFPTIFINAESNLDISENALHSLENDISKYNGRKISLTATLQHVRLLSKIKSFLEDKGYDIVIGKPSNRFMFDGQVLGCDYKAAEVEADIYVIVSGGLFHALGLGLATNKPTIKLDPYLQKSEDITNDVRKILKVRYGKILQALDKRSWVIIQGVKVGQNRPNMIKYFYDKLTEKGYDVFTVTNKVLTLDILRNLDRNYIDVFLVTSCPRLPIDDLYNYEKPVLTPGEAKMIINNSLEPYIFPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q980M0</accession>
    <name>Q980M0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PUA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO5542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90169">
        <property type="entry name" value="C90169"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980M0"/>
    <dbReference type="STRING" id="273057.SSO5542"/>
    <dbReference type="PaxDb" id="273057-SSO5542"/>
    <dbReference type="EnsemblBacteria" id="AAK40610">
        <property type="protein sequence ID" value="AAK40610"/>
        <property type="gene ID" value="SSO5542"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO5542"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.266"/>
    <dbReference type="eggNOG" id="arCOG00991">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2550445_0_0_2"/>
    <dbReference type="InParanoid" id="Q980M0"/>
    <dbReference type="PhylomeDB" id="Q980M0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="39"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="82" mass="9302" checksum="8A769D2CC16F3295" modified="2001-10-01" version="1">MLTSRGELYLVLRAQDVLFSLTLLSGSVIKECSKFPEYRVIIPNSFEEFIKNGRNVFSKHVIAVDKRIRAGDDVIVVNENDS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-03" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Q9</accession>
    <name>RPO5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo5</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo5</name>
        <name evidence="1 3" type="synonym">rpoH</name>
        <name type="ordered locus">SSO5468</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2008" name="Nature" volume="451" first="851" last="854">
            <title>The X-ray crystal structure of RNA polymerase from Archaea.</title>
            <authorList>
                <person name="Hirata A."/>
                <person name="Klein B.J."/>
                <person name="Murakami K.S."/>
            </authorList>
            <dbReference type="PubMed" id="18235446"/>
            <dbReference type="DOI" id="10.1038/nature06530"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF THE RNA POLYMERASE COMPLEX</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 13-subunit RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90164">
        <property type="entry name" value="B90164"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990480.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2PMZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="H/V=1-84"/>
    </dbReference>
    <dbReference type="PDB" id="3HKZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="H/T=1-84"/>
    </dbReference>
    <dbReference type="PDBsum" id="2PMZ"/>
    <dbReference type="PDBsum" id="3HKZ"/>
    <dbReference type="AlphaFoldDB" id="Q980Q9"/>
    <dbReference type="SMR" id="Q980Q9"/>
    <dbReference type="DIP" id="DIP-60641N"/>
    <dbReference type="FunCoup" id="Q980Q9">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="IntAct" id="Q980Q9">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO5468"/>
    <dbReference type="PaxDb" id="273057-SSO5468"/>
    <dbReference type="EnsemblBacteria" id="AAK40569">
        <property type="protein sequence ID" value="AAK40569"/>
        <property type="gene ID" value="SSO5468"/>
    </dbReference>
    <dbReference type="GeneID" id="1455379"/>
    <dbReference type="KEGG" id="sso:SSO5468"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.224"/>
    <dbReference type="eggNOG" id="arCOG04258">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058320_4_0_2"/>
    <dbReference type="InParanoid" id="Q980Q9"/>
    <dbReference type="PhylomeDB" id="Q980Q9"/>
    <dbReference type="BRENDA" id="2.7.7.6">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q980Q9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020608">
        <property type="entry name" value="RNA_pol_subH/Rpb5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01110">
        <property type="entry name" value="RNA_POL_H_23KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000146104" description="DNA-directed RNA polymerase subunit Rpo5">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="14"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="25"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="28"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="41"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="50"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="59"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="63"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="70"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="75"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18235446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="11427726"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="4">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="5">
        <source>
            <dbReference type="PDB" id="3HKZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="3HKZ"/>
        </source>
    </evidence>
    <sequence length="84" mass="9670" checksum="2873A2053F864712" modified="2001-10-01" version="1">MRGSSNKKIDPRIHYLVPKHEVLNIDEAYKILKELGIRPEQLPWIRASDPVARSINAKPGDIIRIIRKSQLYGEVVSYRYVISG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q980W3</accession>
    <name>RS8E_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Small ribosomal subunit protein eS8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">30S ribosomal protein S8e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps8e</name>
        <name type="ordered locus">SSO0164</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7 8 9" key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 3">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90156">
        <property type="entry name" value="F90156"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990388.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2KCO">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-133"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="J=1-133"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="J=1-133"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="J=1-133"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="J=1-133"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="J=1-133"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="J=1-133"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="J=1-133"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="J=1-133"/>
    </dbReference>
    <dbReference type="PDBsum" id="2KCO"/>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q980W3"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q980W3"/>
    <dbReference type="FunCoup" id="Q980W3">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0164"/>
    <dbReference type="PaxDb" id="273057-SSO0164"/>
    <dbReference type="DNASU" id="1453336"/>
    <dbReference type="EnsemblBacteria" id="AAK40509">
        <property type="protein sequence ID" value="AAK40509"/>
        <property type="gene ID" value="SSO0164"/>
    </dbReference>
    <dbReference type="GeneID" id="1453336"/>
    <dbReference type="KEGG" id="sso:SSO0164"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.160"/>
    <dbReference type="eggNOG" id="arCOG04154">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080597_2_1_2"/>
    <dbReference type="InParanoid" id="Q980W3"/>
    <dbReference type="PhylomeDB" id="Q980W3"/>
    <dbReference type="EvolutionaryTrace" id="Q980W3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11382">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.310:FF:000002">
        <property type="entry name" value="30S ribosomal protein S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00029">
        <property type="entry name" value="Ribosomal_eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_eS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018283">
        <property type="entry name" value="Ribosomal_eS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020919">
        <property type="entry name" value="Ribosomal_protein_eS8_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01193">
        <property type="entry name" value="RIBOSOMAL_S8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000122280" description="Small ribosomal subunit protein eS8">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="39"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="62"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="turn" evidence="10">
        <location>
            <begin position="68"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="88"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="turn" evidence="10">
        <location>
            <begin position="92"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="101"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="119"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="2KCO"/>
        </source>
    </evidence>
    <sequence length="133" mass="14659" checksum="90F6D09214FA09D3" modified="2001-10-01" version="1">MGFYQGPDNRKITGGLKGKHRDKRKYEIGNPPTFTTLSAEDIRIKDRTLGGNFKVRLKYTTTANVLDPATNTAKKVKILEILETPANKELARRGIIIRGAKIRTEAGLAVVTSRPGQDGVINAVLLKNESQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Z1</accession>
    <name>Q980Z1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Citramalate synthase</fullName>
            <ecNumber evidence="7">2.3.3.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">leuA-1</name>
        <name evidence="10" type="ordered locus">SSO0127</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from pyruvate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.21" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90153">
        <property type="entry name" value="B90153"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Z1"/>
    <dbReference type="FunCoup" id="Q980Z1">
        <property type="interactions" value="229"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0127"/>
    <dbReference type="PaxDb" id="273057-SSO0127"/>
    <dbReference type="EnsemblBacteria" id="AAK40481">
        <property type="protein sequence ID" value="AAK40481"/>
        <property type="gene ID" value="SSO0127"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0127"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.122"/>
    <dbReference type="eggNOG" id="arCOG02092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_7_0_2"/>
    <dbReference type="InParanoid" id="Q980Z1"/>
    <dbReference type="PhylomeDB" id="Q980Z1"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00066"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043714">
        <property type="term" value="F:(R)-citramalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07941">
        <property type="entry name" value="DRE_TIM_LeuA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005675">
        <property type="entry name" value="Citramal_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00977">
        <property type="entry name" value="citramal_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43538:SF1">
        <property type="entry name" value="(R)-CITRAMALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43538">
        <property type="entry name" value="ALPHA-IPM SYNTHASE/HOMOCITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="9">
        <location>
            <begin position="33"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR00977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK40481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="553" mass="61264" checksum="DC6194F7B6D22282" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q981C2</accession>
    <name>Q981C2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90141">
        <property type="entry name" value="G90141"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989616.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981C2"/>
    <dbReference type="PaxDb" id="273057-SSO0023"/>
    <dbReference type="EnsemblBacteria" id="AAK40390">
        <property type="protein sequence ID" value="AAK40390"/>
        <property type="gene ID" value="SSO0023"/>
    </dbReference>
    <dbReference type="GeneID" id="1455281"/>
    <dbReference type="KEGG" id="sso:SSO0023"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.26"/>
    <dbReference type="eggNOG" id="arCOG05906">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113587_0_0_2"/>
    <dbReference type="InParanoid" id="Q981C2"/>
    <dbReference type="PhylomeDB" id="Q981C2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="205" mass="23887" checksum="DDFD0BE0021868C4" modified="2001-10-01" version="1">MRSKLMFISDLEKLAKRLEELYGDIELDDIVQRLIDFYELGFTNINHSSLQLILSAYFKSLGYRVFVEYEKKGKLLDLYVSDEDMGIEVEYGYIPNSDAIDAEEYLLSRISLKILRYSSLSSKFYIAVPSFYMPPIPEELTADVKDKAKLRRVIEIIRKFYKINDVTFNDVKLAKINGIISVDISNLSISVIDIRKYKQLKDSYK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX97</accession>
    <name>RL14_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl14</name>
        <name evidence="1" type="synonym">rpl14Ab</name>
        <name type="ordered locus">SSO0708</name>
        <name type="ORF">C10_022</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2009" name="Nucleic Acids Res." volume="37" first="256" last="267">
            <title>Function and ribosomal localization of aIF6, a translational regulator shared by archaea and eukarya.</title>
            <authorList>
                <person name="Benelli D."/>
                <person name="Marzi S."/>
                <person name="Mancone C."/>
                <person name="Alonzi T."/>
                <person name="la Teana A."/>
                <person name="Londei P."/>
            </authorList>
            <dbReference type="PubMed" id="19036786"/>
            <dbReference type="DOI" id="10.1093/nar/gkn959"/>
        </citation>
        <scope>INTERACTION WITH INITIATION FACTOR 6 (EIF6)</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L24e, part of which may contact the 16S rRNA in 2 intersubunit bridges (By similarity). Contacts initiation factor aIF-6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90219">
        <property type="entry name" value="B90219"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991271.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX97"/>
    <dbReference type="SMR" id="Q9UX97"/>
    <dbReference type="FunCoup" id="Q9UX97">
        <property type="interactions" value="267"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0708"/>
    <dbReference type="PaxDb" id="273057-SSO0708"/>
    <dbReference type="EnsemblBacteria" id="AAK41009">
        <property type="protein sequence ID" value="AAK41009"/>
        <property type="gene ID" value="SSO0708"/>
    </dbReference>
    <dbReference type="GeneID" id="1454976"/>
    <dbReference type="KEGG" id="sso:SSO0708"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.708"/>
    <dbReference type="eggNOG" id="arCOG04095">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095071_3_0_2"/>
    <dbReference type="InParanoid" id="Q9UX97"/>
    <dbReference type="PhylomeDB" id="Q9UX97"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000007">
        <property type="entry name" value="50S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019971">
        <property type="entry name" value="Ribosomal_uL14_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006344">
        <property type="entry name" value="PRK08571.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03673">
        <property type="entry name" value="uL14_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000128578" description="Large ribosomal subunit protein uL14">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="19036786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="138" mass="15261" checksum="00876DF92F290CEE" modified="2000-05-01" version="1">MSEKIQVLGSRKGLTPALQHYSVVNVADNSGGKEAMIIGVYGYRGVLRRVPFANIADMVMVSIKKGTPEVRKQKFRAVIVRQRMPYRRPDGTWISFEDNAVVIINPDGTPKGTEVRGPIAREAAERWPKIASLATLVV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>P58111</accession>
    <name>TF2B1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription initiation factor IIB 1</fullName>
            <shortName evidence="1">TFIIB 1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tfbA</name>
        <name type="synonym">tfb-1</name>
        <name type="ordered locus">SSO0446</name>
        <name type="ORF">C43_009</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90189">
        <property type="entry name" value="E90189"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988715.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58111"/>
    <dbReference type="SMR" id="P58111"/>
    <dbReference type="FunCoup" id="P58111">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0446"/>
    <dbReference type="PaxDb" id="273057-SSO0446"/>
    <dbReference type="EnsemblBacteria" id="AAK40772">
        <property type="protein sequence ID" value="AAK40772"/>
        <property type="gene ID" value="SSO0446"/>
    </dbReference>
    <dbReference type="GeneID" id="65564463"/>
    <dbReference type="KEGG" id="sso:SSO0446"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.440"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_0_1_2"/>
    <dbReference type="InParanoid" id="P58111"/>
    <dbReference type="PhylomeDB" id="P58111"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20549">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001658">
        <property type="entry name" value="PRK00423.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000119335" description="Transcription initiation factor IIB 1">
        <location>
            <begin position="1"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="repeat" description="1">
        <location>
            <begin position="125"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="repeat" description="2">
        <location>
            <begin position="219"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <sequence length="309" mass="34671" checksum="2AE719C83B93C0DF" modified="2001-06-01" version="1">MLYLSEENKSVSTPCPPDKIIFDAERGEYICSETGEVLEDKIIDQGPEWRAFTPEEKEKRSRVGGPLNNTIHDRGLSTLIDWKDKDAMGRTLDPKRRLEALRWRKWQIRARIQSSIDRNLAQAMNELERIGNLLNLPKSVKDEAALIYRKAVEKGLVRGRSIESVVAAAIYAACRRMKLARTLDEIAQYTKANRKEVARCYRLLLRELDVSVPVSDPKDYVTRIANLLGLSGAVMKTAAEIIDKAKGSGLTAGKDPAGLAAAAIYIASLLHDERRTQKEIAQVAGVTEVTVRNRYKELTQELKISIPTQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-20" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>P58201</accession>
    <name>PCP1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Pyrrolidone-carboxylate peptidase 1</fullName>
            <ecNumber>3.4.19.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>5-oxoprolyl-peptidase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Pyroglutamyl-peptidase I 1</fullName>
            <shortName>PGP-I 1</shortName>
            <shortName>Pyrase 1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pcp1</name>
        <name type="ordered locus">SSO1465</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes 5-oxoproline from various penultimate amino acid residues except L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro.</text>
            <dbReference type="EC" id="3.4.19.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C15 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90304">
        <property type="entry name" value="F90304"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989153.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58201"/>
    <dbReference type="SMR" id="P58201"/>
    <dbReference type="FunCoup" id="P58201">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1465"/>
    <dbReference type="MEROPS" id="C15.001"/>
    <dbReference type="PaxDb" id="273057-SSO1465"/>
    <dbReference type="EnsemblBacteria" id="AAK41693">
        <property type="protein sequence ID" value="AAK41693"/>
        <property type="gene ID" value="SSO1465"/>
    </dbReference>
    <dbReference type="GeneID" id="1454472"/>
    <dbReference type="KEGG" id="sso:SSO1465"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1497"/>
    <dbReference type="eggNOG" id="arCOG05850">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043960_4_3_2"/>
    <dbReference type="InParanoid" id="P58201"/>
    <dbReference type="PhylomeDB" id="P58201"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016920">
        <property type="term" value="F:pyroglutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00501">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.20">
        <property type="entry name" value="Peptidase C15, pyroglutamyl peptidase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00417">
        <property type="entry name" value="Pyrrolid_peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000816">
        <property type="entry name" value="Peptidase_C15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016125">
        <property type="entry name" value="Peptidase_C15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036440">
        <property type="entry name" value="Peptidase_C15-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029762">
        <property type="entry name" value="PGP-I_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033694">
        <property type="entry name" value="PGPEP1_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033693">
        <property type="entry name" value="PGPEP1_Glu_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009672">
        <property type="entry name" value="PRK13193.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402">
        <property type="entry name" value="PROTEASE FAMILY C15 PYROGLUTAMYL-PEPTIDASE I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402:SF1">
        <property type="entry name" value="PYROGLUTAMYL-PEPTIDASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01470">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015592">
        <property type="entry name" value="Prld-crbxl_pptds"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00706">
        <property type="entry name" value="PYROGLUPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53182">
        <property type="entry name" value="Pyrrolidone carboxyl peptidase (pyroglutamate aminopeptidase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01334">
        <property type="entry name" value="PYRASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01333">
        <property type="entry name" value="PYRASE_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0788">Thiol protease</keyword>
    <feature type="chain" id="PRO_0000184758" description="Pyrrolidone-carboxylate peptidase 1">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="211" mass="23681" checksum="84416BE3CB745AB4" modified="2001-06-20" version="1">MTVLLFGFEPFLEYKENPSQLIVEALNRSTILKEEVKGVILPVEYKKIEDVIVTKIRETKPILTLGIGLAPGRAKITPEKIAINYRYSREGDNAGKKYRGEKIDPLGQDGIFTNIPVEDLVDLLNENGIPAELSLSAGSYLCNNAMYIIIREARKYNSLGGFIHVPLHESYAARIQRSIPSMSLDTMIRGIKLSIEFILTNKNKKENLTLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>P60465</accession>
    <name>SECG_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Preprotein translocase subunit SecG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein transport protein Sec61 subunit beta homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">secG</name>
        <name type="ordered locus">SSO5522.1</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in protein export. The function of the beta subunit is unknown, but it may be involved in stabilization of the trimeric complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. Can form oligomers of the heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC61-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990527.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P60465"/>
    <dbReference type="SMR" id="P60465"/>
    <dbReference type="GeneID" id="24780126"/>
    <dbReference type="InParanoid" id="P60465"/>
    <dbReference type="PhylomeDB" id="P60465"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00751">
        <property type="entry name" value="SecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023531">
        <property type="entry name" value="Preprot_translocase_SecG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002318">
        <property type="entry name" value="PRK01253.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000157279" description="Preprotein translocase subunit SecG">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="54"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="55" mass="6136" checksum="544D25DCD0BD9BAF" modified="2004-02-16" version="1">MPSSKKKKETVPVMSMAGLIRYYEEENEKVKISPKIVIGASLALTIIVIVITKLF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1999-07-15" modified="2024-07-24" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>P81552</accession>
    <name>S7C4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Repressor-like protein SSo7c4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0046.1</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="FEBS Lett." volume="432" first="141" last="144">
            <title>Isolation and structure of repressor-like proteins from the archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Oppermann U.C.T."/>
                <person name="Knapp S."/>
                <person name="Bonetto V."/>
                <person name="Ladenstein R."/>
                <person name="Joernvall H."/>
            </authorList>
            <dbReference type="PubMed" id="9720912"/>
            <dbReference type="DOI" id="10.1016/S0014-5793(98)00848-5"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-56</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81552"/>
    <dbReference type="BMRB" id="P81552"/>
    <dbReference type="SMR" id="P81552"/>
    <dbReference type="InParanoid" id="P81552"/>
    <dbReference type="PhylomeDB" id="P81552"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052975">
        <property type="entry name" value="Repressor-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860:SF6">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000097560" description="Repressor-like protein SSo7c4">
        <location>
            <begin position="2"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="4"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AA sequence." evidence="3" ref="2">
        <original>G</original>
        <variation>E</variation>
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1." evidence="3" ref="1">
        <original>DSKTDAH</original>
        <variation>KEPWK</variation>
        <location>
            <begin position="50"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="9720912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="56" mass="6287" checksum="F6E2E32E36232301" modified="2007-01-23" version="2">MAVEEIVKVSRNYQVTIPAKVRQKFQIKEGDLVKVTFDESGGVVKIQLLDSKTDAH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-08" modified="2026-01-28" version="141" xmlns="https://uniprot.org/uniprot">
    <accession>P95960</accession>
    <name>SYFB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="1">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="1">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pheT</name>
        <name type="ordered locus">SSO0101</name>
        <name type="ORF">C04_021</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="1"/>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75388">
        <property type="entry name" value="S75388"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988917.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95960"/>
    <dbReference type="SMR" id="P95960"/>
    <dbReference type="FunCoup" id="P95960">
        <property type="interactions" value="341"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0101"/>
    <dbReference type="PaxDb" id="273057-SSO0101"/>
    <dbReference type="EnsemblBacteria" id="AAK40457">
        <property type="protein sequence ID" value="AAK40457"/>
        <property type="gene ID" value="SSO0101"/>
    </dbReference>
    <dbReference type="GeneID" id="1453285"/>
    <dbReference type="GeneID" id="44129061"/>
    <dbReference type="KEGG" id="sso:SSO0101"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.98"/>
    <dbReference type="eggNOG" id="arCOG00412">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020279_3_0_2"/>
    <dbReference type="InParanoid" id="P95960"/>
    <dbReference type="PhylomeDB" id="P95960"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000014">
        <property type="entry name" value="Phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000003">
        <property type="entry name" value="Phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00284">
        <property type="entry name" value="Phe_tRNA_synth_beta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022918">
        <property type="entry name" value="Phe_tRNA_ligase_beta2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000127012" description="Phenylalanine--tRNA ligase beta subunit">
        <location>
            <begin position="1"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="B5" evidence="1">
        <location>
            <begin position="268"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="544" mass="61816" checksum="4B14A2234BA8ABDE" modified="1997-05-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2025-06-18" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>P95982</accession>
    <name>SYY_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Tyrosyl-tRNA synthetase</fullName>
            <shortName evidence="1">TyrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tyrS</name>
        <name type="ordered locus">SSO0078</name>
        <name type="ORF">C04_044</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="1"/>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75410">
        <property type="entry name" value="S75410"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95982"/>
    <dbReference type="SMR" id="P95982"/>
    <dbReference type="FunCoup" id="P95982">
        <property type="interactions" value="250"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0078"/>
    <dbReference type="PaxDb" id="273057-SSO0078"/>
    <dbReference type="EnsemblBacteria" id="AAK40439">
        <property type="protein sequence ID" value="AAK40439"/>
        <property type="gene ID" value="SSO0078"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0078"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.78"/>
    <dbReference type="eggNOG" id="arCOG01886">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035267_1_1_2"/>
    <dbReference type="InParanoid" id="P95982"/>
    <dbReference type="PhylomeDB" id="P95982"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02009">
        <property type="entry name" value="Tyr_tRNA_synth_type4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023678">
        <property type="entry name" value="Tyr-tRNA-ligase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023617">
        <property type="entry name" value="Tyr-tRNA-ligase_arc/euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050489">
        <property type="entry name" value="Tyr-tRNA_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006330">
        <property type="entry name" value="PRK08560.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264:SF4">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264">
        <property type="entry name" value="TYROSINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006588">
        <property type="entry name" value="TyrRS_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000055671" description="Tyrosine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="241"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02009"/>
        </source>
    </evidence>
    <sequence length="366" mass="41820" checksum="C02CB985642A4D59" modified="1997-05-01" version="1">MGKLIMSIDQRLQLIMRNTAEVITIDELRKKLESDEKLKGYIGFEPSGLFHIGWLIWTQKVKDLVEAGINMTLLRAAWHAWINDKLGGDMNLIKMAADYAVEVIKNYGVDVGKLNIVDADDIVKEKDYWALVIKVAKNASLARIKRALTIMGRRAEEAEIDASKLIYPAMQVSDIFYLDLDIALGGTDQRKAHMLARDVAEKMGKKKIVSIHTPLLVGLQGGQRMNITEGMEEDDIQAEIKMSKSKPESAIFVNDSREDVERKIMGAYCPKGVAENNPILQILKYIIFPRYNFVKIERDIKYGGDVEFKDYEELERTYIEGKIHPMDLKKTTARKLNEILEPIRKSLEKKPEFEEMIQKISKSVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX74</accession>
    <name>Q7LX74_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Second ORF in transposon ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2124</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74036">
        <property type="entry name" value="S74036"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX74"/>
    <dbReference type="STRING" id="273057.SSO2124"/>
    <dbReference type="PaxDb" id="273057-SSO2124"/>
    <dbReference type="EnsemblBacteria" id="AAK42303">
        <property type="protein sequence ID" value="AAK42303"/>
        <property type="gene ID" value="SSO2124"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2124"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2214"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_6_2"/>
    <dbReference type="InParanoid" id="Q7LX74"/>
    <dbReference type="PhylomeDB" id="Q7LX74"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="17"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="120" mass="13546" checksum="5ECAD4025C8BB8BF" modified="2011-05-31" version="1">MGKSLRSLRRRLSDVGFGELRDVLKYQLEKYGKKLILVNPAYTSKTCARCGYVKNDLSLSDRVFVCPNCGWIADRDYNASLNILKSAGSERSLVPVELRPLPVLWHGRAVKQEAPSFMRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX80</accession>
    <name>Q7LX80_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cobalt transport ATP-binding protein (CbiO-2) homolog, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2116</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74028">
        <property type="entry name" value="S74028"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990148.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX80"/>
    <dbReference type="STRING" id="273057.SSO2116"/>
    <dbReference type="PaxDb" id="273057-SSO2116"/>
    <dbReference type="EnsemblBacteria" id="AAK42295">
        <property type="protein sequence ID" value="AAK42295"/>
        <property type="gene ID" value="SSO2116"/>
    </dbReference>
    <dbReference type="GeneID" id="1453622"/>
    <dbReference type="KEGG" id="sso:SSO2116"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2205"/>
    <dbReference type="eggNOG" id="arCOG05372">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_2"/>
    <dbReference type="InParanoid" id="Q7LX80"/>
    <dbReference type="PhylomeDB" id="Q7LX80"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00267">
        <property type="entry name" value="ABC_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43850">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN MA_4021-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43850:SF2">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN MA_4021-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="257" mass="29158" checksum="DC0CEE8F55D8A496" modified="2011-05-31" version="1">MLEYRDFTVKFESVVFQGINMTINRKGIVLGPNGSGKTTIIKATCGLMPYKGYIYVNGMEVRKIRNYLGLSTNLVEVYSIGRKVKDIVYIFEEIKNLDGDMFRRLLEEARLFDQVINKPLYKLSAGQSSIVRLALALSTNPKVALIDEPFENLDPARRLIIARWLKEYFEEGFVTTHELDLLKEFKDWDSYILINGKIYGPIPVTDLIEASVVEGDVENSILTIEIQEGKTLSFVKNAQIGARLTNLGSIDRIYGVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX94</accession>
    <name>Q7LX94_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S78016">
        <property type="entry name" value="S78016"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988663.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX94"/>
    <dbReference type="STRING" id="273057.SSO2101"/>
    <dbReference type="PaxDb" id="273057-SSO2101"/>
    <dbReference type="EnsemblBacteria" id="AAK42280">
        <property type="protein sequence ID" value="AAK42280"/>
        <property type="gene ID" value="SSO2101"/>
    </dbReference>
    <dbReference type="GeneID" id="1453607"/>
    <dbReference type="KEGG" id="sso:SSO2101"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2183"/>
    <dbReference type="eggNOG" id="arCOG03169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061108_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX94"/>
    <dbReference type="PhylomeDB" id="Q7LX94"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301:SF8">
        <property type="entry name" value="ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301">
        <property type="entry name" value="DNA-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="360" mass="41694" checksum="3EBF47D853F66519" modified="2011-05-31" version="1">MVKFIFGRELTEEDEFFDREDYVNVLLSYINRRQPVAILAPRRMGKSSLLNYIKIKLGNEYIVAKISLEGITSIEEFADELTSKLVLEALSKSLKMRAKNTISSLVASLNQFLGSIKSLSIRMQNLELYIDRYSLFKENKLKPSEILDDVLLLPQRIAEDTGKNVVIMIDEFQKIRVLKQPFPKILELTRKRLQESKNVEIIVSGSETGIIEEMITEAKEPFYNYFKIERLKSFDKETSIRFLNEGLKGKCKEYYEKVYELTEGIPAWLNLAGLIFERECNIEAILEDPNVEIELKKDLEGLTKNEIKVLKGLAKGNKLSEIKVSNIYRVIKILKNRGLVDKINDEYKIIDPILSFYLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-06-18" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXH2</accession>
    <name>Q7LXH2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase ISC1476</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO0801</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90230">
        <property type="entry name" value="D90230"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923094.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXH2"/>
    <dbReference type="STRING" id="273057.SSO0801"/>
    <dbReference type="PaxDb" id="273057-SSO0801"/>
    <dbReference type="EnsemblBacteria" id="AAK41099">
        <property type="protein sequence ID" value="AAK41099"/>
        <property type="gene ID" value="SSO0801"/>
    </dbReference>
    <dbReference type="GeneID" id="1455066"/>
    <dbReference type="KEGG" id="sso:SSO0801"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.807"/>
    <dbReference type="eggNOG" id="arCOG00683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040997_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LXH2"/>
    <dbReference type="PhylomeDB" id="Q7LXH2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="225"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="359"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="455" mass="53080" checksum="D1E7556D7AA57CD8" modified="2011-05-31" version="1">MAPSSGQLLGDEEREPTPTPAMPEEGVYEVKYSNRRTNVVRLLPNGFQERKLRRLANLSAKLFNEVNFERRQQFFHEGKVDFKGTWNKYYEKYKTILGVNAQAVLQKNNEAWSSFFSLLKLKKERKLPPHMNRVSPPRYWKDREAGKRKQILVVRQDRYKVDAGNHKDFNMEIDFVGRLKWYGKQGRLEIIFDETKNAWYAHIPIEVGVEETGKKSKHVVKGERKSIQISKPKGNKVTSIDLGINVIASVVVNDGTWLLYKGVRTKEDYFYFQRRISEVQSLADRTRNIGEYDAYLELLREKRRFFKKLTRRLPHLYRNLASHLIETLYELGVSTIYLGYPFNIVQEKGNKFTVNMWSYRKLMESIELKAQEYGMKVFEVVEYNTSKYCAYHDVEVKRNPRGVVNRPLGHKLHSDLNGALNILKKALGVIAEAVKKPLSFIVDHNRVAPIKGCNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXL7</accession>
    <name>Q7LXL7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase M28 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90220">
        <property type="entry name" value="H90220"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991290.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXL7"/>
    <dbReference type="STRING" id="273057.SSO0725"/>
    <dbReference type="PaxDb" id="273057-SSO0725"/>
    <dbReference type="EnsemblBacteria" id="AAK41023">
        <property type="protein sequence ID" value="AAK41023"/>
        <property type="gene ID" value="SSO0725"/>
    </dbReference>
    <dbReference type="GeneID" id="1454990"/>
    <dbReference type="KEGG" id="sso:SSO0725"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.723"/>
    <dbReference type="eggNOG" id="arCOG02960">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_558552_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXL7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014573">
        <property type="entry name" value="UCP032806"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032806">
        <property type="entry name" value="UCP032806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M28" evidence="1">
        <location>
            <begin position="187"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="488" mass="57183" checksum="5CA9161BF57120D9" modified="2011-05-31" version="1">MLLHYLKTLSNYGEIITGSKNETKLVKEIRKILEDNSDEIRLIPIRVLNWEQKELIFECESKLIDAISLPYSLSTEIEADVVDNFKDCNGRNLIKVRIQNLYEINKLYIEAVENNCLGIIFSLDNEKRKFMIKYGDLLSHKPSYPPPIPAFYVKENDFPYIKGKCRISLKTDLNPFATGYIVEAIRNSRNENKIIHITAHHDHWFIGERDNLLAVALLRQFRSNIYETHLISFTAEESGSFNFSTFSWSHGSREFLKNYKNVDDILLNINMDNISEESLVLKTVPGLLELSRKYFKNVVESPEIYSDGYSYIKAGIPSITIESLGNIHYHGEYDIIETDKPNVFSNVTTILDTINKIINENIEYKTHEMRDQLKNNLWELPAELKSYLVNVKDILDKNYTEVYRKIVKLYGGILAFNQPYSKVELFHKIKGLNIARKNDVCIEDLGCIKRLRNDEIYNRFYLYYINELKELITTEYINQLYFILKKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXN9</accession>
    <name>Q7LXN9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90214">
        <property type="entry name" value="F90214"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991216.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXN9"/>
    <dbReference type="STRING" id="273057.SSO0670"/>
    <dbReference type="PaxDb" id="273057-SSO0670"/>
    <dbReference type="EnsemblBacteria" id="AAK40973">
        <property type="protein sequence ID" value="AAK40973"/>
        <property type="gene ID" value="SSO0670"/>
    </dbReference>
    <dbReference type="GeneID" id="1454940"/>
    <dbReference type="KEGG" id="sso:SSO0670"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.671"/>
    <dbReference type="eggNOG" id="arCOG05894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1840449_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXN9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="139" mass="15310" checksum="9D17318DAE398E54" modified="2011-05-31" version="1">MYKVIFRIKGGYGTSFRELRQAGFTPIYFRRDKGEEYYVTIFTGKDLSEVKEAILDLSYYLSKWGKYGDHNFATIYEVKNQNLGKVAGGALGALAGYYLGGLAGLVIGALGGIFLGELVDIEMGEKLVGVLGWPMSISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-03-27" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXQ5</accession>
    <name>Q7LXQ5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxypeptidase regulatory-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90212">
        <property type="entry name" value="D90212"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXQ5"/>
    <dbReference type="STRING" id="273057.SSO0650"/>
    <dbReference type="PaxDb" id="273057-SSO0650"/>
    <dbReference type="EnsemblBacteria" id="AAK40955">
        <property type="protein sequence ID" value="AAK40955"/>
        <property type="gene ID" value="SSO0650"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0650"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.653"/>
    <dbReference type="eggNOG" id="arCOG05997">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1976577_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXQ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15276" checksum="3D940C7E0AF8EB3F" modified="2011-05-31" version="1">MLLMNRQILLALALLVIVVMAIGVYEGNKYRTEISTVALGSQQTDGMYMLKVNVIMNYGPFGGKSPLSNAVIWVYKYNGTTYSFYTYNFTNSQGIASFNLPAGQYKILVTQLRLTYIVNLNQNEEVIINYAYLNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXT5</accession>
    <name>Q7LXT5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0582</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90204">
        <property type="entry name" value="H90204"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991097.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXT5"/>
    <dbReference type="STRING" id="273057.SSO0582"/>
    <dbReference type="PaxDb" id="273057-SSO0582"/>
    <dbReference type="EnsemblBacteria" id="AAK40895">
        <property type="protein sequence ID" value="AAK40895"/>
        <property type="gene ID" value="SSO0582"/>
    </dbReference>
    <dbReference type="GeneID" id="1454862"/>
    <dbReference type="KEGG" id="sso:SSO0582"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.590"/>
    <dbReference type="eggNOG" id="arCOG05992">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_854237_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXT5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="325" mass="36906" checksum="2557359DCCCA1FBC" modified="2011-05-31" version="1">MPKGELAIFDEGDFEGSIDYQSFTKSIKLSNINKEASFSIDIASKPTKFYLIIQAKREKTYLPRWRLWFDNFSLTKEFKPNLETEINDAQVISTIVYDITPIIKEGKHQIAVYHPSIQVLELLNISLISFYRTEGFSTKYRLSAGSLIFNRNDSVSFHLMKKSYFVIRNESKEGMVKISDIDGKTIYTILPSSDSDEIEIENNDAITTTLQSNNEKDYGVILASYNLLEKAPKINFSVDSKVSDNIIYLYLKNESEISLDKLLINVMINGVPAHFKTFTNIDVSSTIDLKLPISVPQSKKAPIINVRVVGVKGGYRKIIDKQISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXW4</accession>
    <name>Q7LXW4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0529</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90199">
        <property type="entry name" value="B90199"/>
    </dbReference>
    <dbReference type="PIR" id="S63531">
        <property type="entry name" value="S63531"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991047.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXW4"/>
    <dbReference type="SMR" id="Q7LXW4"/>
    <dbReference type="STRING" id="273057.SSO0529"/>
    <dbReference type="PaxDb" id="273057-SSO0529"/>
    <dbReference type="EnsemblBacteria" id="AAK40849">
        <property type="protein sequence ID" value="AAK40849"/>
        <property type="gene ID" value="SSO0529"/>
    </dbReference>
    <dbReference type="GeneID" id="1454816"/>
    <dbReference type="KEGG" id="sso:SSO0529"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.528"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_16_5_2"/>
    <dbReference type="InParanoid" id="Q7LXW4"/>
    <dbReference type="PhylomeDB" id="Q7LXW4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF25">
        <property type="entry name" value="USPA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="13"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="136" mass="15103" checksum="6F89A75D4864E3AE" modified="2011-05-31" version="1">MSEPSYFVSFWLRKILVPVDGSENSLRALDLAVDFGMRYGSKITIIHVCSDCNNMNDIQSLIEKRINNKIEYDLKIVKINIKESSVSNEILKVINEEPYDAIIMGARGTSLNSDINIGSTALAISINAPVSVILVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXX9</accession>
    <name>Q7LXX9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75372">
        <property type="entry name" value="S75372"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXX9"/>
    <dbReference type="STRING" id="273057.SSO0117"/>
    <dbReference type="PaxDb" id="273057-SSO0117"/>
    <dbReference type="EnsemblBacteria" id="AAK40472">
        <property type="protein sequence ID" value="AAK40472"/>
        <property type="gene ID" value="SSO0117"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0117"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.112"/>
    <dbReference type="eggNOG" id="arCOG07276">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156340_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXX9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF046074">
        <property type="entry name" value="UpsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="137" mass="15479" checksum="8BB29552844F6110" modified="2011-05-31" version="1">MMWLKAISSIFSTLIVVMITLSLIVPLYLFFTQTYTNSSIQANSAYDNYLTNVNVKVSLIYLGNSVNETFVYNYGSVPITIEKVIINNISYNVKYEMLPDSLVPLSSIINNNIFIEGNSVIILQINGNYYYFNSGPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXZ0</accession>
    <name>Q7LXZ0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF1512 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0097</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75438">
        <property type="entry name" value="S75438"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922884.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXZ0"/>
    <dbReference type="SMR" id="Q7LXZ0"/>
    <dbReference type="STRING" id="273057.SSO0097"/>
    <dbReference type="PaxDb" id="273057-SSO0097"/>
    <dbReference type="EnsemblBacteria" id="AAK40453">
        <property type="protein sequence ID" value="AAK40453"/>
        <property type="gene ID" value="SSO0097"/>
    </dbReference>
    <dbReference type="GeneID" id="1453281"/>
    <dbReference type="KEGG" id="sso:SSO0097"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.94"/>
    <dbReference type="eggNOG" id="arCOG04181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062565_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXZ0"/>
    <dbReference type="PhylomeDB" id="Q7LXZ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009995">
        <property type="entry name" value="DUF1512"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056461">
        <property type="entry name" value="DUF1512_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056460">
        <property type="entry name" value="DUF1512_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07431">
        <property type="entry name" value="DUF1512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23542">
        <property type="entry name" value="DUF1512_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016495">
        <property type="entry name" value="UCP016495"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1512" evidence="2">
        <location>
            <begin position="18"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1512" evidence="3">
        <location>
            <begin position="201"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="41603" checksum="08C9467CAE5BCF0E" modified="2011-05-31" version="1">MSLIALAQTSLNQANSLYYILTYVLFFVLLFLLYLPGVNTRLTVSMLARGIEGQLSMIEKYLNESKSKMEQLLKERGVQDPKPFIERISEMFIIDPVNVEPTDIISRMRLLLRSGEDKIRDLITLMVPNIDNVNRSKLEVSAEVVNSLNLIYKVVRHYLILAKKLNSAILLYQLQFVVPQLVKISEAYSKAMNTFIRGIPVGDSLGPLVAAYLFMKADKKWNPSRDTVAGEVELEGRKLIVVKAEGPMATVGRPGEAVENVVEEYKGKVTRIITIDAALKLEGENTGAIAEGTGVAMGDPGPEKISIERVAVKYNIPIDAVIVKMSMEEAITEMRKEIYQAAPKALELVKKIILERTKPGDIVVVVGVGNTAGVAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-10-02" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LY06</accession>
    <name>Q7LY06_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrogenase expression/formation protein (HypE)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hypE</name>
        <name evidence="4" type="ordered locus">SSO0052</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75435">
        <property type="entry name" value="S75435"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LY06"/>
    <dbReference type="STRING" id="273057.SSO0052"/>
    <dbReference type="PaxDb" id="273057-SSO0052"/>
    <dbReference type="EnsemblBacteria" id="AAK40418">
        <property type="protein sequence ID" value="AAK40418"/>
        <property type="gene ID" value="SSO0052"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0052"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.56"/>
    <dbReference type="eggNOG" id="arCOG00636">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041631_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LY06"/>
    <dbReference type="PhylomeDB" id="Q7LY06"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06061">
        <property type="entry name" value="PurM-like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011854">
        <property type="entry name" value="HypE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303:SF4">
        <property type="entry name" value="HYDROGENASE EXPRESSION_FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303">
        <property type="entry name" value="HYDROGENASE ISOENZYMES FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005644">
        <property type="entry name" value="Hdrgns_mtr_HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="2">
        <location>
            <begin position="52"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="3">
        <location>
            <begin position="165"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="343" mass="37590" checksum="A26B4CDB69F4AD47" modified="2011-05-31" version="1">MYSLYKSLTSTITLVIYANYFIIVNVNFGKVPINKFIYNLPIGDCLTCPSIGEDAAVLEAKDKYLIVHSDPITEAKSDSGFLSIAVACNDINMKGAQCRWVNSIILLSDISHLNATISAISEACNLIGCKVVGGHTEVSDKVKQDIIITTAIGVSNKFLSYKNVKEGMKVVLVGSPGIEGTWILAKDYDQLLLEKGISREIIEKAKEFKKDIVVQPKALSILDYVVAMHDATEGGIMQAMLEVAKASGYTVSINLDKIVLRRETKIITKALNIDPLRLISSGSFIAVSNTPEKILERISEAYIIGEIKRGDPVLEIKGVGTFNQDFEEELVKFESNYLGWGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TV7</accession>
    <name>Q97TV7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ORF in partial transposon ISC774</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="3">Second ORF in transposon ISC774</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO10122</name>
        <name evidence="3" type="ordered locus">SSO11417</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90397">
        <property type="entry name" value="C90397"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TV7"/>
    <dbReference type="PaxDb" id="273057-SSO10122"/>
    <dbReference type="EnsemblBacteria" id="AAK42434">
        <property type="protein sequence ID" value="AAK42434"/>
        <property type="gene ID" value="SSO10122"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK43006">
        <property type="protein sequence ID" value="AAK43006"/>
        <property type="gene ID" value="SSO11417"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO10122"/>
    <dbReference type="KEGG" id="sso:SSO11417"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2364"/>
    <dbReference type="eggNOG" id="arCOG02134">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2230495_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="84" mass="9837" checksum="790774677C2B487D" modified="2001-10-01" version="1">MHDGLSVYNAFDWFNVGHKRVTLGDYTEEGFRKLKYRVSSMDFACNSNKLMSWFSTFFLIYNLLYIQVYLVDKGVMINANISTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TX0</accession>
    <name>Q97TX0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">First ORF in transposon ISC1491</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="4">Second ORF in transposon ISC1491</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1237</name>
        <name evidence="4" type="ordered locus">SSO1697</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90278">
        <property type="entry name" value="B90278"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TX0"/>
    <dbReference type="STRING" id="273057.SSO1237"/>
    <dbReference type="PaxDb" id="273057-SSO1237"/>
    <dbReference type="EnsemblBacteria" id="AAK41481">
        <property type="protein sequence ID" value="AAK41481"/>
        <property type="gene ID" value="SSO1237"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41906">
        <property type="protein sequence ID" value="AAK41906"/>
        <property type="gene ID" value="SSO1697"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1237"/>
    <dbReference type="KEGG" id="sso:SSO1697"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1235"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098916_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="152" mass="17493" checksum="063B0ECD23F70DFE" modified="2001-10-01" version="1">MKEVFGKKTDKLDAQRLAKAFILGAVKGSYIPTGEIRELRELTRYRESLMRKITQVKNEIRKFLEMAGYKIEPFDKRGMALLEKLSRGEGLSKEERDELKEKLGRSLNDAEKLALKQLVDLLKSLEAMVKEVEDMIISKIPQPVVELSRELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TX2</accession>
    <name>Q97TX2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">First ORF in transposon ISC1078</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0040</name>
        <name evidence="3" type="ordered locus">SSO1264</name>
        <name evidence="4" type="ordered locus">SSO1750</name>
        <name evidence="5" type="ordered locus">SSO2992</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90143">
        <property type="entry name" value="F90143"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TX2"/>
    <dbReference type="STRING" id="273057.SSO0040"/>
    <dbReference type="PaxDb" id="273057-SSO0040"/>
    <dbReference type="EnsemblBacteria" id="AAK40405">
        <property type="protein sequence ID" value="AAK40405"/>
        <property type="gene ID" value="SSO0040"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41502">
        <property type="protein sequence ID" value="AAK41502"/>
        <property type="gene ID" value="SSO1264"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41946">
        <property type="protein sequence ID" value="AAK41946"/>
        <property type="gene ID" value="SSO1750"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK43098">
        <property type="protein sequence ID" value="AAK43098"/>
        <property type="gene ID" value="SSO2992"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0040"/>
    <dbReference type="KEGG" id="sso:SSO1264"/>
    <dbReference type="KEGG" id="sso:SSO1750"/>
    <dbReference type="KEGG" id="sso:SSO2992"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1261"/>
    <dbReference type="eggNOG" id="arCOG02127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1718298_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051839">
        <property type="entry name" value="RD_transcriptional_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025959">
        <property type="entry name" value="Winged_HTH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33215">
        <property type="entry name" value="PROTEIN DISTAL ANTENNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33215:SF13">
        <property type="entry name" value="PROTEIN DISTAL ANTENNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13592">
        <property type="entry name" value="HTH_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Winged helix-turn helix" evidence="1">
        <location>
            <begin position="89"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="152" mass="18047" checksum="031C79AE35310A66" modified="2001-10-01" version="1">MPKSRMHGIRLNKKQEEERRINAVKDVRNGMSIKDVAKKYDVSIFTVYKWLRKGDLSAKPRKGPTKLKDEEKLVRILEKSPRDFGLNYDFWTLKLIAYILDKEFGIKYNPRSLSPVLKKLGFKYKKGKRTYVRDDNAVERWVKEQGEKLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TX7</accession>
    <name>Q97TX7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycogen debranching enzyme, hypothetical (TreX-like)</fullName>
            <ecNumber evidence="3">3.2.1.33</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">treX-like</name>
        <name evidence="3" type="ordered locus">SSO0991</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.2.1.33" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90250">
        <property type="entry name" value="H90250"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TX7"/>
    <dbReference type="STRING" id="273057.SSO0991"/>
    <dbReference type="PaxDb" id="273057-SSO0991"/>
    <dbReference type="EnsemblBacteria" id="AAK41263">
        <property type="protein sequence ID" value="AAK41263"/>
        <property type="gene ID" value="SSO0991"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0991"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.988"/>
    <dbReference type="eggNOG" id="arCOG03287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026835_0_0_2"/>
    <dbReference type="InParanoid" id="Q97TX7"/>
    <dbReference type="PhylomeDB" id="Q97TX7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004134">
        <property type="term" value="F:4-alpha-glucanotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004135">
        <property type="term" value="F:amylo-alpha-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010401">
        <property type="entry name" value="AGL/Gdb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032790">
        <property type="entry name" value="GDE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006451">
        <property type="entry name" value="Glycogen_debranch_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024742">
        <property type="entry name" value="Glycogen_debranch_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01561">
        <property type="entry name" value="gde_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569:SF2">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06202">
        <property type="entry name" value="GDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12439">
        <property type="entry name" value="GDE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycogen debranching enzyme bacterial and archaeal type N-terminal" evidence="2">
        <location>
            <begin position="10"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme C-terminal" evidence="1">
        <location>
            <begin position="251"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="544" mass="63755" checksum="4B4C78D5FE4B6E99" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2013-06-26" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U30</accession>
    <name>GAPN_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="2">1.2.1.90</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Glyceraldehyde phosphate dehydrogenase (NAD(P))</fullName>
            <shortName>GAPN</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">gapN-3</name>
        <name type="ordered locus">SSO3194</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Biochem. J." volume="390" first="529" last="540">
            <title>The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluation.</title>
            <authorList>
                <person name="Ahmed H."/>
                <person name="Ettema T.J."/>
                <person name="Tjaden B."/>
                <person name="Geerling A.C."/>
                <person name="van der Oost J."/>
                <person name="Siebers B."/>
            </authorList>
            <dbReference type="PubMed" id="15869466"/>
            <dbReference type="DOI" id="10.1042/BJ20041711"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the irreversible NAD(P)-dependent non-phosphorylating oxidation of glyceraldehyde-3-phosphate (GAP) to 3-phosphoglycerate (3PG). It is highly specific for D-GAP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>D-glyceraldehyde 3-phosphate + NADP(+) + H2O = (2R)-3-phosphoglycerate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.90"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>D-glyceraldehyde 3-phosphate + NAD(+) + H2O = (2R)-3-phosphoglycerate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42760"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.90"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by AMP, ADP, glucose 1-phosphate (G1P), and fructose 6-phosphate (F6P).</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">0.099 mM for NADP (at 70 degrees Celsius)</KM>
            <KM evidence="2">0.453 mM for GAP (at 70 degrees Celsius)</KM>
            <Vmax evidence="2">3.3 umol/min/mg enzyme with NADP as substrate (at 70 degrees Celsius)</Vmax>
            <Vmax evidence="2">3.4 umol/min/mg enzyme with GAP as substrate (at 70 degrees Celsius)</Vmax>
        </kinetics>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer. Dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.90" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90504">
        <property type="entry name" value="E90504"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991691.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U30"/>
    <dbReference type="SMR" id="Q97U30"/>
    <dbReference type="FunCoup" id="Q97U30">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3194"/>
    <dbReference type="PaxDb" id="273057-SSO3194"/>
    <dbReference type="EnsemblBacteria" id="AAK43292">
        <property type="protein sequence ID" value="AAK43292"/>
        <property type="gene ID" value="SSO3194"/>
    </dbReference>
    <dbReference type="GeneID" id="1453211"/>
    <dbReference type="KEGG" id="sso:SSO3194"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3298"/>
    <dbReference type="eggNOG" id="arCOG01252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_0_2"/>
    <dbReference type="InParanoid" id="Q97U30"/>
    <dbReference type="PhylomeDB" id="Q97U30"/>
    <dbReference type="BRENDA" id="1.2.1.9">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q97U30"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043878">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008886">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (non-phosphorylating) activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd07082">
        <property type="entry name" value="ALDH_F11_NP-GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000010">
        <property type="entry name" value="N-succinylglutamate 5-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000022">
        <property type="entry name" value="NADP-dependent glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051020">
        <property type="entry name" value="ALDH-related_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053489">
        <property type="entry name" value="NAD(P)-GAP_dehydrogenase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040869">
        <property type="entry name" value="G3PDH_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42991">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42991:SF1">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0021">Allosteric enzyme</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000422653" description="NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>alpha-D-glucose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>beta-D-fructose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>alpha-D-glucose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>beta-D-fructose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="161"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>alpha-D-glucose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="161"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="198"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="303"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="463"/>
            <end position="464"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O57693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15869466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="509" mass="56927" checksum="A9D47247B08400E7" modified="2001-10-01" version="1">MEKTSVLIKSKELMEIYELKDGVPYFKTYLSGQWISGDEWQDVISPIDLNIIGKIPKLNWNQIDDTLEHIYRKGRWSIRDTPGEKRLDIYKKMASLLDKFKEDFVNVLMINNGKTKSAAEGEVKAAIERLLRADLDVKETRGDYVPGDWSSETLETEAVVRKEPVGVVLSIVPFNYPLFDTVNKIVYTTVIGNAIIIKPPSSTPLPILMLAKVMELASFPKDSFAIITIPGRDMNKVVGDKRIQAISLTGSTETGEEVVRNAGIKQFIMELGGGDPAIVLSDADLAWAAQRIAAGIISYTGQRCDSVKLVLVEEEVYDTLKDLLIKELTKSVKVGDPRDPLTTVGPVIDVKTVDEWEKAIKDAVEKGGKILFGGKRLGPTYIEPVLIEAPKETLKDMYFYNKEVFASAALLIKVKNIDEALEISNSRKYGLDAAIFGKDINKIRKLQRFLEVGAIYINDYPRHGIGYFPFGGRKDSGIGREGIGYTIQYVTAYKSIVYNYKGKGIWEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U72</accession>
    <name>Q97U72_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="2">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO3148</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90499">
        <property type="entry name" value="B90499"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924147.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U72"/>
    <dbReference type="FunCoup" id="Q97U72">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3148"/>
    <dbReference type="PaxDb" id="273057-SSO3148"/>
    <dbReference type="EnsemblBacteria" id="AAK43249">
        <property type="protein sequence ID" value="AAK43249"/>
        <property type="gene ID" value="SSO3148"/>
    </dbReference>
    <dbReference type="GeneID" id="1453168"/>
    <dbReference type="KEGG" id="sso:SSO3148"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3253"/>
    <dbReference type="eggNOG" id="arCOG01067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_815399_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U72"/>
    <dbReference type="PhylomeDB" id="Q97U72"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF47">
        <property type="entry name" value="EXTERNAL NADH-UBIQUINONE OXIDOREDUCTASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="8">
        <location>
            <begin position="5"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK43249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="350" mass="38523" checksum="AF137FBB100C13E6" modified="2001-10-01" version="1">MQGERVVILGGGFAGIAAKLVYPNAILVDEKDFMVVTPRLVEVIEKNLPLSYALIHRKVDVKAKILKVDFKEKKVITTNGEIKFDKLIIALGYEQDLSKIKGAENYGIGFTLENIEKIKSFKEGSVVTILGGGALGVELAGALRKRGHIVNLVEAESRLLPYLVSDFSREVQRVLENQGVNVILSAKVEEVNQNEVVTTQGRIRSDYVIFSAGFSGPKIIKEMALSNKNNRMLVDRYLRSIDYDFVYGAGDCANFKDGFIPQSAQVSLQAGEVAMNNAIKDEKVEFKPVQRAIVLKIGEDYIGLIKNTVISGPIASFIKSFAISSLEGKVKKVNRLILAVFTRGVTSAYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UG1</accession>
    <name>Q97UG1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">alpha-L-fucosidase</fullName>
            <ecNumber evidence="2">3.2.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fucA1</name>
        <name evidence="7" type="ordered locus">SSO11867</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 29 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.51" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90488">
        <property type="entry name" value="A90488"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UG1"/>
    <dbReference type="SMR" id="Q97UG1"/>
    <dbReference type="STRING" id="273057.SSO11867"/>
    <dbReference type="CAZy" id="GH29">
        <property type="family name" value="Glycoside Hydrolase Family 29"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO11867"/>
    <dbReference type="EnsemblBacteria" id="AAK43160">
        <property type="protein sequence ID" value="AAK43160"/>
        <property type="gene ID" value="SSO11867"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11867"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3158"/>
    <dbReference type="eggNOG" id="arCOG07338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2629896_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UG1"/>
    <dbReference type="SABIO-RK" id="Q97UG1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004560">
        <property type="term" value="F:alpha-L-fucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000933">
        <property type="entry name" value="Glyco_hydro_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057739">
        <property type="entry name" value="Glyco_hydro_29_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10030">
        <property type="entry name" value="ALPHA-L-FUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10030:SF37">
        <property type="entry name" value="ALPHA-L-FUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01120">
        <property type="entry name" value="Alpha_L_fucos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycoside hydrolase family 29 N-terminal" evidence="6">
        <location>
            <begin position="15"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="81" mass="9590" checksum="8999C4145700A848" modified="2001-10-01" version="1">MSQNSYKILKSLPVPSNGPFKPTWSSLKKYIVPSWFTTSKFGIFIHWGVYSVPAFGNEWYPRYMYMPDRPEHQYHLKNSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UN4</accession>
    <name>Q97UN4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Quinol oxidase-2, subunit II (SoxH)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">soxH</name>
        <name evidence="10" type="ordered locus">SSO2969</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c oxidase subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90477">
        <property type="entry name" value="C90477"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924080.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UN4"/>
    <dbReference type="FunCoup" id="Q97UN4">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2969"/>
    <dbReference type="PaxDb" id="273057-SSO2969"/>
    <dbReference type="EnsemblBacteria" id="AAK43074">
        <property type="protein sequence ID" value="AAK43074"/>
        <property type="gene ID" value="SSO2969"/>
    </dbReference>
    <dbReference type="GeneID" id="1452993"/>
    <dbReference type="KEGG" id="sso:SSO2969"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3060"/>
    <dbReference type="eggNOG" id="arCOG01235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1763905_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UN4"/>
    <dbReference type="PhylomeDB" id="Q97UN4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098803">
        <property type="term" value="C:respiratory chain complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045187">
        <property type="entry name" value="CcO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001505">
        <property type="entry name" value="Copper_CuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888:SF9">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888">
        <property type="entry name" value="CYTOCHROME C OXIDASE, SUBUNIT II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00116">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00078">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit II copper A binding" evidence="9">
        <location>
            <begin position="45"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK43074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="146" mass="16483" checksum="8BD81AB59BAAF2C6" modified="2001-10-01" version="1">MEKARIFELSTIVFAIVILTVLGVFSDIYLNSINTGAYLSTTQRQDAIPIKVVAMQYAWEFIYPNGTTTTDQLVLKANQTYLLEITSKDVIHAFYIPQLGFKFEAIPGYVYDFYIVVNKPGVYDIWCAEFCGPGHYLMKGTLVVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UN6</accession>
    <name>Q97UN6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4367 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2967</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90477">
        <property type="entry name" value="A90477"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924078.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UN6"/>
    <dbReference type="STRING" id="273057.SSO2967"/>
    <dbReference type="PaxDb" id="273057-SSO2967"/>
    <dbReference type="EnsemblBacteria" id="AAK43072">
        <property type="protein sequence ID" value="AAK43072"/>
        <property type="gene ID" value="SSO2967"/>
    </dbReference>
    <dbReference type="GeneID" id="1452991"/>
    <dbReference type="KEGG" id="sso:SSO2967"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3058"/>
    <dbReference type="eggNOG" id="arCOG07230">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1773283_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UN6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="15901" checksum="E41E9E7A851A2FB3" modified="2001-10-01" version="1">MKKGKIVVPIVVMVIAALVVLSSGVLTVVNPFISTSAVSRELGGSWSVQNYYSGNNTVTEILIDSQGNVLVITQYSFPSVTQAQSFFYANSLGQVEQYENYLISYYSHGLSESLYILNGGKVYYISYVATTSTSMPSISQLLSLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UQ8</accession>
    <name>Q97UQ8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Second ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2944</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90474">
        <property type="entry name" value="A90474"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924047.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UQ8"/>
    <dbReference type="STRING" id="273057.SSO2944"/>
    <dbReference type="PaxDb" id="273057-SSO2944"/>
    <dbReference type="EnsemblBacteria" id="AAK43048">
        <property type="protein sequence ID" value="AAK43048"/>
        <property type="gene ID" value="SSO2944"/>
    </dbReference>
    <dbReference type="GeneID" id="1452967"/>
    <dbReference type="KEGG" id="sso:SSO2944"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3032"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="InParanoid" id="Q97UQ8"/>
    <dbReference type="PhylomeDB" id="Q97UQ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF22">
        <property type="entry name" value="TNPB-LIKE PROTEIN MJ1635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="188"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="309"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK43048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="424" mass="50403" checksum="ADA8280B160103AC" modified="2001-10-01" version="1">MLKNLRIRKFEPEEEYVYFTYSIKNDKREKSKELIREYTTLLQKAIDYLWNLTKIQVRKKNGNYKITLPKKREVYKPLREELEKINHLASHYVDKAINDAFSILKSWRKRAIKGRASIEKPRVKKAYVRIKTTLRKVVGESVRITVRPHEYITFSWSKSWFSRRVRELELGEPIIKEEKVYLPFRYKLPWATPVDFLAIDSNLYTLDAYDGEKFVTISLKQLYSLKYSMEVKRAKVQSFASKHTKRGRELMRKYSHRERNRVLDFVHKFVNTLLDLYPMTFFAVEKLNKESMFKDANDSLSRKISRTVWGSIHRVLKYKAPLYGSFVKEVNPRFTSRSCPRCGFVSRKVGKTFECERCGFKLDRQLNASLNIYLKMCGFPHIREIARVWVGVIPLMGRRGMNGFPRDFGEAQGLRIDIEYHEIP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-16" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UU4</accession>
    <name>Y2899_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein SSO2899</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2899</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99469">
        <property type="entry name" value="A99469"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993055.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UU4"/>
    <dbReference type="SMR" id="Q97UU4"/>
    <dbReference type="FunCoup" id="Q97UU4">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2899"/>
    <dbReference type="PaxDb" id="273057-SSO2899"/>
    <dbReference type="EnsemblBacteria" id="AAK43008">
        <property type="protein sequence ID" value="AAK43008"/>
        <property type="gene ID" value="SSO2899"/>
    </dbReference>
    <dbReference type="GeneID" id="1452927"/>
    <dbReference type="KEGG" id="sso:SSO2899"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2989"/>
    <dbReference type="eggNOG" id="arCOG04225">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046550_7_0_2"/>
    <dbReference type="InParanoid" id="Q97UU4"/>
    <dbReference type="PhylomeDB" id="Q97UU4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02907">
        <property type="entry name" value="Macro_Af1521_BAL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001665">
        <property type="entry name" value="PRK00431.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001667">
        <property type="entry name" value="PRK00431.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001668">
        <property type="entry name" value="PRK00431.2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106">
        <property type="entry name" value="GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106:SF111">
        <property type="entry name" value="MACRO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000089237" description="Uncharacterized protein SSO2899">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00490"/>
        </source>
    </evidence>
    <sequence length="177" mass="19445" checksum="8353685B14FECA96" modified="2001-10-01" version="1">MYKNPYGLEIYLIKGDITEIEADAIVNAANSYLQHGGGVAYAIVRKGGYIIQKESDEYVKKFGPVPVGEVAVTSAGKLKAKYVIHAVGPRYGIEGEDKLESAIFKSLLKADELSLSSIAMPAISTGIYGYPFEICARIMANVLKGYKPKTLRKVMICLYTKDAYDVFKSIFNSILKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V09</accession>
    <name>Q97V09_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3211 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2828</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90460">
        <property type="entry name" value="C90460"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923986.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V09"/>
    <dbReference type="PaxDb" id="273057-SSO2828"/>
    <dbReference type="EnsemblBacteria" id="AAK42938">
        <property type="protein sequence ID" value="AAK42938"/>
        <property type="gene ID" value="SSO2828"/>
    </dbReference>
    <dbReference type="GeneID" id="1452857"/>
    <dbReference type="KEGG" id="sso:SSO2828"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2913"/>
    <dbReference type="eggNOG" id="arCOG07265">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2103620_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V09"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021578">
        <property type="entry name" value="STK_08120-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11485">
        <property type="entry name" value="STK_08120-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="16273" checksum="E116DE03E472AE77" modified="2001-10-01" version="1">MEWRKIIMTNHDIDAVIKILSDPEFFIPLVLPVTKFLLNSDSRLYRCEGDIGKLPVVFQGREYVGSYNITHIIEFERPPNVSGKINIQAIENKIVITILLQNLYIIYLPLRVHISNRLNQLEKTFEEKIRLERIKRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V29</accession>
    <name>Q97V29_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ABM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO11196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90457">
        <property type="entry name" value="G90457"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991448.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V29"/>
    <dbReference type="STRING" id="273057.SSO11196"/>
    <dbReference type="PaxDb" id="273057-SSO11196"/>
    <dbReference type="EnsemblBacteria" id="AAK42918">
        <property type="protein sequence ID" value="AAK42918"/>
        <property type="gene ID" value="SSO11196"/>
    </dbReference>
    <dbReference type="GeneID" id="1452837"/>
    <dbReference type="KEGG" id="sso:SSO11196"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2891"/>
    <dbReference type="eggNOG" id="arCOG05403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182726_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V29"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004392">
        <property type="term" value="F:heme oxygenase (decyclizing) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042167">
        <property type="term" value="P:heme catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050404">
        <property type="entry name" value="Heme-degrading_MO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34474">
        <property type="entry name" value="SIGNAL TRANSDUCTION PROTEIN TRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34474:SF2">
        <property type="entry name" value="SIGNAL TRANSDUCTION PROTEIN TRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="2"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="97" mass="11463" checksum="26DAFD079B8A9547" modified="2001-10-01" version="1">MINVGFYYKVKRGFEKDFENKFKEVLSYLRANADGFVDAKLYKNVDEPSEYLIYSVWRDLDSFKKFVSSSAYKNTVNYGKSIIESKPIHRILQEIND</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V58</accession>
    <name>Q97V58_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PhoU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2777</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90453">
        <property type="entry name" value="H90453"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990219.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V58"/>
    <dbReference type="STRING" id="273057.SSO2777"/>
    <dbReference type="PaxDb" id="273057-SSO2777"/>
    <dbReference type="EnsemblBacteria" id="AAK42887">
        <property type="protein sequence ID" value="AAK42887"/>
        <property type="gene ID" value="SSO2777"/>
    </dbReference>
    <dbReference type="GeneID" id="1452806"/>
    <dbReference type="KEGG" id="sso:SSO2777"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2859"/>
    <dbReference type="eggNOG" id="arCOG07224">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2067920_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V58"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13656" checksum="12A17C63BFB2BF96" modified="2001-10-01" version="1">MFGKLVAVIDKLNEGNVIEAGNELLLIAKDYEDQDKIIDLLAEIEKEIKEFKSSNDFLHRDDSPFMDMVKRSMEEMRICRENKLKALILHTLYIISNGNEILLNMIKKVNIGKPNTYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VJ3</accession>
    <name>Q97VJ3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase (Flavoprotein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90436">
        <property type="entry name" value="H90436"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988532.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VJ3"/>
    <dbReference type="FunCoup" id="Q97VJ3">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2629"/>
    <dbReference type="PaxDb" id="273057-SSO2629"/>
    <dbReference type="EnsemblBacteria" id="AAK42751">
        <property type="protein sequence ID" value="AAK42751"/>
        <property type="gene ID" value="SSO2629"/>
    </dbReference>
    <dbReference type="GeneID" id="1452670"/>
    <dbReference type="KEGG" id="sso:SSO2629"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2713"/>
    <dbReference type="eggNOG" id="arCOG01064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030742_5_0_2"/>
    <dbReference type="InParanoid" id="Q97VJ3"/>
    <dbReference type="PhylomeDB" id="Q97VJ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052541">
        <property type="entry name" value="SQRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755:SF1">
        <property type="entry name" value="FAD-DEPENDENT PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="4"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="385" mass="43054" checksum="8EA14A9FB69F60C5" modified="2001-10-01" version="1">MAKRVIIAGGNIAGTIVANRLVQKLEHEVNKGDVEIVALNKSDEHIYLPGQLLVGFGLETPGELVRKESELLDPRIKFLHGQKGTISKIDVANHSVQTADGVSHSYDYLVITTGVDYTWDEIPGYRSAAHTVYEYDDAIKMREALEKFDGGTIVVNTAKLPHRCPVAPLEVTLILDDYLRKRGIRDKTKIIYTYPVQGVFGRPITNKFMLRLFEQRGIEVHSPFTVTSVDPNNKVIESQEGEKLKFDMLIGIPPNMGAKVIEDSGIGDRRRWVPTDKFTLRMKDHSNVYVMGDATDLPVSKAGSTADFESYTVAHNIANDIKGNLGVKHYGGDVLCYIATGTDQATYIRFSYTMNESPPPPSYVHWWGKIMYNKMYWTVTAKAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VK3</accession>
    <name>Q97VK3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aminobenzoate oxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2621</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90435">
        <property type="entry name" value="F90435"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989345.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VK3"/>
    <dbReference type="SMR" id="Q97VK3"/>
    <dbReference type="STRING" id="273057.SSO2621"/>
    <dbReference type="PaxDb" id="273057-SSO2621"/>
    <dbReference type="DNASU" id="1454068"/>
    <dbReference type="EnsemblBacteria" id="AAK42741">
        <property type="protein sequence ID" value="AAK42741"/>
        <property type="gene ID" value="SSO2621"/>
    </dbReference>
    <dbReference type="GeneID" id="1454068"/>
    <dbReference type="KEGG" id="sso:SSO2621"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2703"/>
    <dbReference type="eggNOG" id="arCOG06036">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_827971_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VK3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="39581" checksum="C6EA0E2089BA27F2" modified="2001-10-01" version="1">MDINYNDDPIYPEDNVYPPAWEFDNEKAMRLYRRAKKEQWDEEDLDWKKYEEIISGLDRKQRLALAYWWALLSNFDNATPVFAYALVKASEKRLPVTIKALLTTITFDENRHNVLCGFAIDKAMKGFPFNFKPQDDLERKAKLNVEWVWWNGSRYWKGYIEAYNKYTLDILFTSFMMGEAAATTVFSGMKDKTKIPTFKDAFRNLTVDETRHYAFTHLILTDNLNKMNEEKKMFVTKQIRAGFVFLSLITYKPPKEFWKLPPWYKEVHEKMEELAIDAGLGLPTIEERERIWREAVARVASNVKRFNVKVPSMPEIGINGDEDIELKEDDLVASIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VL7</accession>
    <name>Q97VL7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR-associated protein, MJ1666 family</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2606</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90433">
        <property type="entry name" value="H90433"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989325.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VL7"/>
    <dbReference type="PaxDb" id="273057-SSO2606"/>
    <dbReference type="EnsemblBacteria" id="AAK42727">
        <property type="protein sequence ID" value="AAK42727"/>
        <property type="gene ID" value="SSO2606"/>
    </dbReference>
    <dbReference type="GeneID" id="1454054"/>
    <dbReference type="KEGG" id="sso:SSO2606"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2687"/>
    <dbReference type="eggNOG" id="arCOG03436">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_651549_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VL7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160980">
        <property type="entry name" value="SSO1389-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="48202" checksum="3349DE1B3EAA76FD" modified="2001-10-01" version="1">MKILIIAPLTEVSEEENEIRPIEVTNWSNSPVGISSKYSFLAEKKFLEEERHEVKILTLVPTEIRKNLNVKFSNYNELVSNILEALKFGNDNIAVEVIPFEDSVSLGTSLFFSYLKIYYAIKTFLPSLIILDVSHSSSSFSTLVLTALDIAITDSLLTQQVENYIYAKVSKRNDSIQLISHMFKNMYSIKLSEYFLREMKIMKSENQANLPQFVGRAEFRRLGFCIENCYPLVVLHVLDGMDLEKLVSEEKIMEIVVNNLEIKDGKLIQNVELLEGATYYVLATHLVKRYKVKKPFSVDNVRNILNLTSPTCRRISNQIIDEMMIELNYLLRHLGLREGEYSLEQISNLLKQPLTEIIRKEESIGDILSRYKRECGSINLSGIGLDPGATIIKIDSDKIYVYYADECEDSVLSKVKDQMGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VR9</accession>
    <name>Q97VR9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Partial transposase ISC1058</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO10604</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99427">
        <property type="entry name" value="A99427"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VR9"/>
    <dbReference type="STRING" id="273057.SSO10604"/>
    <dbReference type="PaxDb" id="273057-SSO10604"/>
    <dbReference type="EnsemblBacteria" id="AAK42672">
        <property type="protein sequence ID" value="AAK42672"/>
        <property type="gene ID" value="SSO10604"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO10604"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2623"/>
    <dbReference type="eggNOG" id="arCOG02754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102439_1_0_2"/>
    <dbReference type="InParanoid" id="Q97VR9"/>
    <dbReference type="PhylomeDB" id="Q97VR9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053172">
        <property type="entry name" value="Tn903_transposase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631:SF3">
        <property type="entry name" value="ISSOD12 TRANSPOSASE TNPA_ISSOD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="97" mass="11914" checksum="0B6D3D89565A19AC" modified="2001-10-01" version="1">MGKSKYKRDWSKYDENVITRYKLMFPFYVFEHWWDLLAEENRNARTKYKAPKEFNDFLAFLHIFLPYRAIEGVLRALEQLKIIPTSLDYSRECPKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VU6</accession>
    <name>Q97VU6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase (Acd-4)</fullName>
            <ecNumber evidence="10">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">acd-4</name>
        <name evidence="10" type="ordered locus">SSO2511</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="10"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90423">
        <property type="entry name" value="E90423"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988491.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VU6"/>
    <dbReference type="STRING" id="273057.SSO2511"/>
    <dbReference type="PaxDb" id="273057-SSO2511"/>
    <dbReference type="EnsemblBacteria" id="AAK42644">
        <property type="protein sequence ID" value="AAK42644"/>
        <property type="gene ID" value="SSO2511"/>
    </dbReference>
    <dbReference type="GeneID" id="1453971"/>
    <dbReference type="KEGG" id="sso:SSO2511"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2590"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_9_0_2"/>
    <dbReference type="InParanoid" id="Q97VU6"/>
    <dbReference type="PhylomeDB" id="Q97VU6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000011">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052161">
        <property type="entry name" value="Mycobact_Acyl-CoA_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43292">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43292:SF4">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE FADE34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="122"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="262"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="370" mass="41512" checksum="BEB739011A8DCD9A" modified="2001-10-01" version="1">MSKNEDELEEYRIKVREWIRNNVPEEIRAKGDMAPPDVLREWQRRIYEAGYLGVSWPKEYGGWGENPIKEIIVREEFAKAGVPYATVGLGVSVVGPAIILNGTEEQKKKHIRRILTAEDVWCQGFSEPQAGSDLAGIKTKAEDKGEYFLVNGQKIWSSYAHLANYCLLLTRTGDVSERHKGLTMLIVDMKSEGIRVSPIKQITGRSEFNTVYFNNVKVPKENVVGKIGEGWKVAMSTLNYERLNIGTILFTVERLIRELSINNDQLFNTAEEIVALKSFYKRILERLKKGYIAGPEAAVIKLVASEAIQRVYENAMANMGIEGLVMEREPGFRPEIAYGFLASRSITIAGGTSEILRNLLGEVVLGLPKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VX1</accession>
    <name>Q97VX1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Succinyl-CoA synthetase, beta subunit (SucC)</fullName>
            <ecNumber evidence="7">6.2.1.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sucC</name>
        <name evidence="7" type="ordered locus">SSO2483</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.5" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90420">
        <property type="entry name" value="D90420"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923887.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VX1"/>
    <dbReference type="FunCoup" id="Q97VX1">
        <property type="interactions" value="360"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2483"/>
    <dbReference type="PaxDb" id="273057-SSO2483"/>
    <dbReference type="EnsemblBacteria" id="AAK42619">
        <property type="protein sequence ID" value="AAK42619"/>
        <property type="gene ID" value="SSO2483"/>
    </dbReference>
    <dbReference type="GeneID" id="7808238"/>
    <dbReference type="KEGG" id="sso:SSO2483"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2564"/>
    <dbReference type="eggNOG" id="arCOG01337">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037430_0_2_2"/>
    <dbReference type="InParanoid" id="Q97VX1"/>
    <dbReference type="PhylomeDB" id="Q97VX1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_ligase-like_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF10">
        <property type="entry name" value="SUCCINATE--COA LIGASE [GDP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="6">
        <location>
            <begin position="9"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="337" mass="37354" checksum="6C78EC36A489289B" modified="2001-10-01" version="1">MKLYEYEGKSLFKRVGIPVPNGVVTSEPIRWQGKAVVKSQLLEGARGKRGLVRVTEDIYNTILELKKLGVEKFLVEEFVPHEKEIYASVLLDRETAEPMLVLSREGGIDVEQAKDVKKMIIPLERGVRSYDIVEAEKYLGVKGLGQIIQGLYKLFVDYDAELVEINPLALTNDGRILALDSKVILEDNALYRHEDLLKELGRQEVRDSYVELEGDIGIIGNGAGLTMASMDLVKLNGGNPANFLDVGGGANREHVKESVLKVGSNPRVKKIVINIYGGITRCDEVALGIIDALKEVKKPIFVRLLGTNEELGKKILRENGVNVYDDVLKMIGDAVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VZ1</accession>
    <name>Q97VZ1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF47 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0111 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90417">
        <property type="entry name" value="H90417"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923867.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VZ1"/>
    <dbReference type="STRING" id="273057.SSO2460"/>
    <dbReference type="PaxDb" id="273057-SSO2460"/>
    <dbReference type="EnsemblBacteria" id="AAK42599">
        <property type="protein sequence ID" value="AAK42599"/>
        <property type="gene ID" value="SSO2460"/>
    </dbReference>
    <dbReference type="GeneID" id="7806401"/>
    <dbReference type="KEGG" id="sso:SSO2460"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2537"/>
    <dbReference type="eggNOG" id="arCOG02640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1259117_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VZ1"/>
    <dbReference type="PhylomeDB" id="Q97VZ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002727">
        <property type="entry name" value="DUF47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018445">
        <property type="entry name" value="Put_Phosphate_transp_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36536">
        <property type="entry name" value="UPF0111 PROTEIN HI_1603"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36536:SF3">
        <property type="entry name" value="UPF0111 PROTEIN HI_1603"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01865">
        <property type="entry name" value="PhoU_div"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="25073" checksum="12D4901A13A0B139" modified="2001-10-01" version="1">MFKLRLNNKEEEVFERISFTAELIGEAVKILQNEVNHVRKGENDQIPADLIKIKSIHERAGMLREEILSTLYGEAFLPDFKESMVMLTQALFNTLSSVKDAARALGSRKVDLKCVTILSDMLITYLALVNEASLKIHELTRHLGSDVEVSLKLSREIQAMEREGDDIKDTLLQRLYEIEKEIDIISILQMKDVIIFIDDILDSLEDATLSVEVFYATLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VZ2</accession>
    <name>Q97VZ2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF763 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2459</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90417">
        <property type="entry name" value="G90417"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923866.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VZ2"/>
    <dbReference type="STRING" id="273057.SSO2459"/>
    <dbReference type="PaxDb" id="273057-SSO2459"/>
    <dbReference type="EnsemblBacteria" id="AAK42598">
        <property type="protein sequence ID" value="AAK42598"/>
        <property type="gene ID" value="SSO2459"/>
    </dbReference>
    <dbReference type="GeneID" id="7806400"/>
    <dbReference type="KEGG" id="sso:SSO2459"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2536"/>
    <dbReference type="eggNOG" id="arCOG04253">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061722_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VZ2"/>
    <dbReference type="PhylomeDB" id="Q97VZ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008482">
        <property type="entry name" value="DUF763"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38597">
        <property type="entry name" value="BLL3834 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38597:SF1">
        <property type="entry name" value="BLL3834 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05559">
        <property type="entry name" value="DUF763"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="42977" checksum="7D0756B88D78EAD8" modified="2001-10-01" version="1">MEVEGIADLPLHTGHVPPWLVPIMKRLAKAIVEIMILELGPQKVVERFSNPLWFQGFNNIIGMDWDSSGSTTVTLGILKDITNPKVHGFAVLGGKGKNALKVPDEVDMLIFDVDKNKIKNISKIVAKVDSTLVQDGHQLYHHTMLVTEDGKWSIIQQGMNLEIKFARRYHWKETDNFVVEPHSAISGIKTNVVVNVIDRDKDRTRKLILDLLRENPNKIVSLYTQAMAMLKGQSTLDMWIKGGSIAFVSKEARLIYMKPVDVNRIKQVLREMYESNPLTFEEALINGLGPSTARALYLIADLIYNEPPSYKDPMNYPYDPFKYAFAIGGKDGIPYPVNRKAAWEVIYTLEDFITRAKLEEGDKRLALRKLKELSKGIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W67</accession>
    <name>Q97W67_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90408">
        <property type="entry name" value="B90408"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989472.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W67"/>
    <dbReference type="STRING" id="273057.SSO2370"/>
    <dbReference type="PaxDb" id="273057-SSO2370"/>
    <dbReference type="EnsemblBacteria" id="AAK42521">
        <property type="protein sequence ID" value="AAK42521"/>
        <property type="gene ID" value="SSO2370"/>
    </dbReference>
    <dbReference type="GeneID" id="1453848"/>
    <dbReference type="KEGG" id="sso:SSO2370"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2455"/>
    <dbReference type="eggNOG" id="arCOG05955">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1493010_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W67"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="179" mass="20322" checksum="F470C4948E148746" modified="2001-10-01" version="1">MSTEIDSKNSSIDMFTTYEEELRVGEALAHILAAASIVLELEGESEEVRNTIMKYIDLWISKLSPIDYSPGMAEVIGSKVRRKITSVFNEISENELGDILDFIIDVKRKLDIGTLETEILELEVKVERILRVLGIDINDVKQFFDFTNLEKRANRLIALATVSIGIASVWDEKWTVELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W86</accession>
    <name>Q97W86_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2351</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90405">
        <property type="entry name" value="G90405"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989504.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W86"/>
    <dbReference type="STRING" id="273057.SSO2351"/>
    <dbReference type="PaxDb" id="273057-SSO2351"/>
    <dbReference type="EnsemblBacteria" id="AAK42502">
        <property type="protein sequence ID" value="AAK42502"/>
        <property type="gene ID" value="SSO2351"/>
    </dbReference>
    <dbReference type="GeneID" id="42363406"/>
    <dbReference type="KEGG" id="sso:SSO2351"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2435"/>
    <dbReference type="eggNOG" id="arCOG05959">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1500346_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W86"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20627" checksum="B1BA667DD539A744" modified="2001-10-01" version="1">MKILVMSNIRFPEPHVESTLSSIIKKEEPEVIVLNGDTTQCYWDYECPRVIDVLYVIRSIAPWAQIIYVQGDMDPHAIKCITAEPRYREEIIGTTMYIAEAASVKYHIIHGHQGEIDQLRKSIGAGPWDWLVIGQYKRLEIDKLARVLYSGGITREFPPEARGYVVITDSNFYIRNLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WB2</accession>
    <name>Q97WB2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2323</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Flagellin is the subunit protein which polymerizes to form the filaments of archaeal flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the archaeal flagellin family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90402">
        <property type="entry name" value="E90402"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WB2"/>
    <dbReference type="STRING" id="273057.SSO2323"/>
    <dbReference type="PaxDb" id="273057-SSO2323"/>
    <dbReference type="EnsemblBacteria" id="AAK42476">
        <property type="protein sequence ID" value="AAK42476"/>
        <property type="gene ID" value="SSO2323"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2323"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2410"/>
    <dbReference type="eggNOG" id="arCOG01829">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_948700_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WB2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903:SF1">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Flagellin_arch-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0974">Archaeal flagellum</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="307" mass="33364" checksum="6B0C814EA8861B76" modified="2001-10-01" version="1">MNSKKMLKEYNKKVKRKGLAGLDTAIILIAFIITASVLAYVAINMGLFVTQKAKSTINKGEETASTALTLSGSVLYAVNYPLNTRSYWIYFTVSPSSGVSSVELSPTTTAISFTASAEGVTYSNIYKYTLLTVSPSELANVVYANGQYLDLVNQQTSAGQTYVYYPNPYYALLALNYTLYNYYLSTKTPSPIFINSSILSLSSLPSWLKNDNSFTFTLNISGKLVTYYVFVNQTFAFTYPVAGDPLIGSAIAPAGSVIGVILLFGPDLGSHVFQYQTITIQITPNIGSPLTISEYIYQPEGSVSVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WC3</accession>
    <name>Q97WC3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cofactor biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2309</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90401">
        <property type="entry name" value="B90401"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991800.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WC3"/>
    <dbReference type="STRING" id="273057.SSO2309"/>
    <dbReference type="PaxDb" id="273057-SSO2309"/>
    <dbReference type="EnsemblBacteria" id="AAK42465">
        <property type="protein sequence ID" value="AAK42465"/>
        <property type="gene ID" value="SSO2309"/>
    </dbReference>
    <dbReference type="GeneID" id="1453792"/>
    <dbReference type="KEGG" id="sso:SSO2309"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2398"/>
    <dbReference type="eggNOG" id="arCOG00938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_5_2"/>
    <dbReference type="InParanoid" id="Q97WC3"/>
    <dbReference type="PhylomeDB" id="Q97WC3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000410">
        <property type="entry name" value="Radical SAM domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022563">
        <property type="entry name" value="DUF3463"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023863">
        <property type="entry name" value="rSAM_PTO1314"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03961">
        <property type="entry name" value="rSAM_PTO1314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF7">
        <property type="entry name" value="PQQA PEPTIDE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11946">
        <property type="entry name" value="DUF3463"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="22"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="344" mass="39900" checksum="023BE4B7CAF38A7F" modified="2001-10-01" version="1">MSTLKPMIFGNVKRALLGLGVRKLPLIAGHKLLYTCNLRCRMCPFWRRKDEKLLSLEEEVLMLKSLEKAGVLFMGFEGGEPLLRRDLEDILEESYKRFYTSLVTNGWLLKDRAKSISNYLDYLFVSIDGIGEVHDKIRGISGAFERAVEGIKEARKYLPVAISFTITKENMDQVKDVIELARKLNVNISIQVEYDYSTAEKLSPDKRRLYDALRLIIELKKRGYQIIESSDYFEAIIKSWYNGIPWRCKPWLTINIDPQGRIVLPCYVLNEYSGTDKVWEVDIVKLWNSYPWEKYESCNACALACYLEPSLFSWSNGKMVKEKIIDNMASYVYNASPIKFLLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WE4</accession>
    <name>Q97WE4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2282</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90398">
        <property type="entry name" value="E90398"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991834.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WE4"/>
    <dbReference type="STRING" id="273057.SSO2282"/>
    <dbReference type="PaxDb" id="273057-SSO2282"/>
    <dbReference type="EnsemblBacteria" id="AAK42444">
        <property type="protein sequence ID" value="AAK42444"/>
        <property type="gene ID" value="SSO2282"/>
    </dbReference>
    <dbReference type="GeneID" id="1453771"/>
    <dbReference type="KEGG" id="sso:SSO2282"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2375"/>
    <dbReference type="eggNOG" id="arCOG05898">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149090_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WE4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="145" mass="16310" checksum="27C2E020DCC73FD0" modified="2001-10-01" version="1">MKAVERLDNTMVELNKINESELGINELDLLRFLKNQLSKSKSLFESFSKSIDEKRWDDVLSYTFQISQRVNSIFGYLVQPAVFSIISKSKLSENIENIIDSLAFSISEMIIVLKQNNKSLGIDTITINMSSNPPSMSISVVIKGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2017-03-15" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WF1</accession>
    <name>UDGA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type-4 uracil-DNA glycosylase</fullName>
            <ecNumber evidence="2">3.2.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">UDG1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">udg1</name>
        <name evidence="5" type="ordered locus">SSO2275</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Biochem. J." volume="387" first="859" last="863">
            <title>Characterization of an archaeal family 4 uracil DNA glycosylase and its interaction with PCNA and chromatin proteins.</title>
            <authorList>
                <person name="Dionne I."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="15588253"/>
            <dbReference type="DOI" id="10.1042/BJ20041661"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>INTERACTION WITH PCNA3</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Removes uracil bases that are present in DNA as a result of either deamination of cytosine or misincorporation of dUMP instead of dTMP. Can remove uracil from double-stranded DNA containing either a U/G or U/A base pair as well as from single-stranded DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 65 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with the sliding clamp PCNA3 subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 4 (UDGa) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90397">
        <property type="entry name" value="F90397"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991844.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WF1"/>
    <dbReference type="SMR" id="Q97WF1"/>
    <dbReference type="STRING" id="273057.SSO2275"/>
    <dbReference type="PaxDb" id="273057-SSO2275"/>
    <dbReference type="EnsemblBacteria" id="AAK42437">
        <property type="protein sequence ID" value="AAK42437"/>
        <property type="gene ID" value="SSO2275"/>
    </dbReference>
    <dbReference type="GeneID" id="1453764"/>
    <dbReference type="KEGG" id="sso:SSO2275"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2367"/>
    <dbReference type="eggNOG" id="arCOG00905">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044815_1_3_2"/>
    <dbReference type="InParanoid" id="Q97WF1"/>
    <dbReference type="PhylomeDB" id="Q97WF1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd10030">
        <property type="entry name" value="UDG-F4_TTUDGA_SPO1dp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.470.10:FF:000013">
        <property type="entry name" value="Type-4 uracil-DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053423">
        <property type="entry name" value="Type-4_UDG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051536">
        <property type="entry name" value="UDG_Type-4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005273">
        <property type="entry name" value="Ura-DNA_glyco_family4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040953">
        <property type="entry name" value="Arch_udg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00758">
        <property type="entry name" value="UDG_fam4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693:SF1">
        <property type="entry name" value="TYPE-4 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693">
        <property type="entry name" value="TYPE-5 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000439185" description="Type-4 uracil-DNA glycosylase">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="41"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>uracil</name>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>uracil</name>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>uracil</name>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>uracil</name>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q5SKC5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15588253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="15588253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="216" mass="24498" checksum="10C6122573C245DB" modified="2001-10-01" version="1">MDNLDLIADEVRKCQKCKLWKFRKNAVPGEGNSKAEIMFIGEAPGENEDIEGKPFVGVAGKLLTRLINEILGLSREDVFITNLVKCRPPNNRDPEEDEILACSPYLTRQIESIRPHIIITLGRHSTSYLFKKMNMKMESIGKVRGKFYTWNIYGYKILVFPTYHPAAALYNPPIRKVLEEDFRKVKEALSSKPITLDNFLYGSGDKGEKGNSNSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WL1</accession>
    <name>Q97WL1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-oxoacyl-(Acyl carrier protein) reductase (FabG-4)</fullName>
            <ecNumber evidence="3">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fabG-4</name>
        <name evidence="3" type="ordered locus">SSO2205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90389">
        <property type="entry name" value="H90389"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992081.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WL1"/>
    <dbReference type="STRING" id="273057.SSO2205"/>
    <dbReference type="PaxDb" id="273057-SSO2205"/>
    <dbReference type="EnsemblBacteria" id="AAK42375">
        <property type="protein sequence ID" value="AAK42375"/>
        <property type="gene ID" value="SSO2205"/>
    </dbReference>
    <dbReference type="GeneID" id="1453702"/>
    <dbReference type="KEGG" id="sso:SSO2205"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2301"/>
    <dbReference type="eggNOG" id="arCOG01262">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_2"/>
    <dbReference type="InParanoid" id="Q97WL1"/>
    <dbReference type="PhylomeDB" id="Q97WL1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051122">
        <property type="entry name" value="SDR_DHRS6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004453">
        <property type="entry name" value="PRK05786.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477">
        <property type="entry name" value="DIHYDROANTICAPSIN 7-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477:SF1">
        <property type="entry name" value="DIHYDROANTICAPSIN 7-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="239" mass="25945" checksum="4247371B007D28AD" modified="2001-10-01" version="1">MGRLDNKKVAIIGVSEGLGYAVAYFALKEGAQVCINARNENKLKKMKETLSKYGNIQYVVGDVSNIEGARNVLDKCASFLNGIDHLVVTVGGYIEDTIDHFSGLEEMLTNHIKIPLYVINSSLRFLREGSSVVLVSSMSGIGKASPDQLSYAVAKAGLAKEVEIIASELLGKGIRVNGIAPTTIGGEFEPERNWKKLRKLGDDMAPPEDFAKVIIWLLTDESEWVDGVVIPVHGGARLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WN0</accession>
    <name>Q97WN0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">isocitrate dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="4">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">idh</name>
        <name evidence="17" type="ordered locus">SSO2182</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90387">
        <property type="entry name" value="E90387"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WN0"/>
    <dbReference type="FunCoup" id="Q97WN0">
        <property type="interactions" value="189"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2182"/>
    <dbReference type="PaxDb" id="273057-SSO2182"/>
    <dbReference type="EnsemblBacteria" id="AAK42356">
        <property type="protein sequence ID" value="AAK42356"/>
        <property type="gene ID" value="SSO2182"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2182"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2279"/>
    <dbReference type="eggNOG" id="arCOG01164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_7_1_2"/>
    <dbReference type="InParanoid" id="Q97WN0"/>
    <dbReference type="PhylomeDB" id="Q97WN0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004439">
        <property type="entry name" value="Isocitrate_DH_NADP_dimer_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005036">
        <property type="entry name" value="PRK06451.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43504">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43504:SF1">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0521">NADP</keyword>
    <keyword evidence="9 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="16">
        <location>
            <begin position="26"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="333"/>
            <end position="339"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="14">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="14">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-succinyllysine" evidence="15">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="15">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="15">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AAK42356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="413" mass="46451" checksum="97C7C5357D1E83C5" modified="2001-10-01" version="1">MMQKIPEDGEVIKFENGKWIVPKKPIILYVEGDGIGHEITHAAMKVINKAVEKAYGSDREIKWVEVLAGDKAEKLTGNRFPKESEELIEKYRVLLKGPLETPIGKGWKSINVAIRLMLDLYANIRPVKYIPGIESPIKNADKIDLIIFRENTDDLYRGIEYPYDSEQAKKIRDFLRKELGVEVEDDTGIGIKLISRFKTQRIARMAIKYAIDHKRKKVTIMHKGNVMKYTEGAFREWSYEVATKEFRDYIVTEEEVTKNYNGVPPSGKVIINDRIADNMFQQIIIRPDEYDIILAPNVNGDYISDAAGALVGNIGMLGGANIGDTGGMFEAIHGTAPKYAGKNVANPTGIIKGGELMLRFMGWDKAAELIDSAIMESIRQKKVTQDLARFMGVRALSTTEYTDELIAIIDTLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-15" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WN9</accession>
    <name>NADE_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NH(3)-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="1">6.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nadE</name>
        <name type="ordered locus">SSO2172</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>deamido-NAD(+) + NH4(+) + ATP = AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK42347" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.3.1.5" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42347.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90386">
        <property type="entry name" value="D90386"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WN9"/>
    <dbReference type="SMR" id="Q97WN9"/>
    <dbReference type="FunCoup" id="Q97WN9">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2172"/>
    <dbReference type="PaxDb" id="273057-SSO2172"/>
    <dbReference type="EnsemblBacteria" id="AAK42347">
        <property type="protein sequence ID" value="AAK42347"/>
        <property type="gene ID" value="SSO2172"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2172"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2267"/>
    <dbReference type="eggNOG" id="arCOG00069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059327_1_1_2"/>
    <dbReference type="InParanoid" id="Q97WN9"/>
    <dbReference type="PhylomeDB" id="Q97WN9"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00333"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000106">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00193">
        <property type="entry name" value="NadE_ammonia_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022926">
        <property type="entry name" value="NH(3)-dep_NAD(+)_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010587">
        <property type="entry name" value="PRK13980.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152235" description="NH(3)-dependent NAD(+) synthetase">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="252"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="278" mass="31593" checksum="8D3E6061E4E66C3A" modified="2002-11-15" version="2">MHEYIRKSLTIDCEAVTNYIVERIREYLEFSNKKGGVIGVSGGVDSAVTATLLAKATDNFFILLMPSSSTPKIDLDDSFEMIKFLNAQNKYKLINIDEIVKSFSNKIETENKYIIGNIKARVRMIILYAYAQMLDYLVVGTGDKSELLLGYFTKYGDGGVDVLPIGDLYKTQVRMLGKCLGLPERIVTKPSSPALWEGQTAEGELGIDYETIDSILYLRFDEMRSEDEIVKMLGIPIDIVKKVDRLVKISQHKRLPPEIFRLSGRAINSDWRFPRRWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WY2</accession>
    <name>Q97WY2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90364">
        <property type="entry name" value="B90364"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WY2"/>
    <dbReference type="PaxDb" id="273057-SSO1978"/>
    <dbReference type="EnsemblBacteria" id="AAK42169">
        <property type="protein sequence ID" value="AAK42169"/>
        <property type="gene ID" value="SSO1978"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1978"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2055"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113529_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WY2"/>
    <dbReference type="PhylomeDB" id="Q97WY2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025471">
        <property type="entry name" value="DUF4322"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14210">
        <property type="entry name" value="DUF4322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4322" evidence="1">
        <location>
            <begin position="2"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="251" mass="28352" checksum="8CE2653D8262C3F1" modified="2001-10-01" version="1">MVTPGLPHQNNIQQIGYKLLSMLNFKGKKGEEVARTLISACLWNDSVESKSRAYGVSPQTVTNYVEKQGVEVIEKLLESARKISLKVLKGVKEIDLSIDWTTKIWYGRPVGGLGSSEEGNSWNYATATTKFNGKVLLLAFVTQVKGMTKEEIVKALVEQVVAMGFKIRLMTLDAGFYTIDVLNFVSQFKYVIGVPVGDVKVYQEFDGEYETNSKRHRRDEQVKFRLLVYSKEKVKRKRKTLVYLGLLGIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X04</accession>
    <name>Q97X04_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90361">
        <property type="entry name" value="D90361"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991755.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X04"/>
    <dbReference type="STRING" id="273057.SSO1956"/>
    <dbReference type="PaxDb" id="273057-SSO1956"/>
    <dbReference type="EnsemblBacteria" id="AAK42147">
        <property type="protein sequence ID" value="AAK42147"/>
        <property type="gene ID" value="SSO1956"/>
    </dbReference>
    <dbReference type="GeneID" id="7806413"/>
    <dbReference type="KEGG" id="sso:SSO1956"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2029"/>
    <dbReference type="eggNOG" id="arCOG05999">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1551727_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X04"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="179" mass="20985" checksum="EA1DDC60916CD9AD" modified="2001-10-01" version="1">MIVTKRTLILTISMLLNVLVALLPGYWWYYSAGGMVVIKDSLFSFYLEFLGKELEIGIIINYILFAFRFYVISVSLYYIYLALKKDVINNYLLITWISYLYLLDPLLFYLLFNYVVGYVTPTKYPLFIIGSQNMTVFYKNVMVTILVESYPTTYYWIALFAGTFNLISRIIISRLSKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X20</accession>
    <name>Q97X20_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1932</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99359">
        <property type="entry name" value="A99359"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992186.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X20"/>
    <dbReference type="STRING" id="273057.SSO1932"/>
    <dbReference type="PaxDb" id="273057-SSO1932"/>
    <dbReference type="EnsemblBacteria" id="AAK42128">
        <property type="protein sequence ID" value="AAK42128"/>
        <property type="gene ID" value="SSO1932"/>
    </dbReference>
    <dbReference type="GeneID" id="7807179"/>
    <dbReference type="KEGG" id="sso:SSO1932"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2007"/>
    <dbReference type="eggNOG" id="arCOG07311">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987483_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X20"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="143" mass="16566" checksum="3505D00CDDBE334F" modified="2001-10-01" version="1">MNISSQNKIYTVKRNLILEYCKTPKSFTELRDLTGMSDAGLSKALNDLIKKGYLQKTSDGKYVITDKVMQSYKERIVNRIWFKYQGISDEKIEKIADLLKDEGEFYILASKGKDKTDDLTLLLQYLFLLTYEIEGSKELKVTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X53</accession>
    <name>Q97X53_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1902</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90354">
        <property type="entry name" value="F90354"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X53"/>
    <dbReference type="PaxDb" id="273057-SSO1902"/>
    <dbReference type="EnsemblBacteria" id="AAK42093">
        <property type="protein sequence ID" value="AAK42093"/>
        <property type="gene ID" value="SSO1902"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1902"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1960"/>
    <dbReference type="eggNOG" id="arCOG07718">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1567243_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X53"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="183" mass="21501" checksum="6EC60390A2E606A0" modified="2001-10-01" version="1">MEIIQHNYRKYLKMVDIAKCFKGVEDFITSSAKNKRWEKLWNIIKECEKINFFELNIETLIGRCVRGELEVCEKLGLNNEQLKKLVENPYVVNFSFIMVDIFIKEFLDLILITSYLEPLNTITRYSISSESKQSEKELLDDAVENQDKIMSFLEVKVQVVKNLIENNEFVENLNQLYEILGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X89</accession>
    <name>Q97X89_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIP18 family-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90349">
        <property type="entry name" value="F90349"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988467.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X89"/>
    <dbReference type="FunCoup" id="Q97X89">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1866"/>
    <dbReference type="PaxDb" id="273057-SSO1866"/>
    <dbReference type="EnsemblBacteria" id="AAK42053">
        <property type="protein sequence ID" value="AAK42053"/>
        <property type="gene ID" value="SSO1866"/>
    </dbReference>
    <dbReference type="GeneID" id="1453380"/>
    <dbReference type="KEGG" id="sso:SSO1866"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1915"/>
    <dbReference type="eggNOG" id="arCOG01845">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091588_2_2_2"/>
    <dbReference type="InParanoid" id="Q97X89"/>
    <dbReference type="PhylomeDB" id="Q97X89"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052339">
        <property type="entry name" value="Fe-S_Maturation_MIP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831:SF1">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="1">
        <location>
            <begin position="11"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="129" mass="14990" checksum="8B848876A4C52500" modified="2001-10-01" version="1">MNKKEKGEWKKKIIQGLHEVYDPEIPIDIVNLGLIYQLDISDEGDVYVRIGATTPACPVTEDLEYTVEQVVKESVQAKSIKVELDLETEWTPLMMTDEGRKEFIRKFGYDIVKRWAERMGIKLEEEKVT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X95</accession>
    <name>HTPX1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protease HtpX homolog 1</fullName>
            <ecNumber evidence="1">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">htpX1</name>
        <name type="synonym">htpX-1</name>
        <name type="ordered locus">SSO1859</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M48B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90348">
        <property type="entry name" value="H90348"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988523.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X95"/>
    <dbReference type="FunCoup" id="Q97X95">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1859"/>
    <dbReference type="PaxDb" id="273057-SSO1859"/>
    <dbReference type="EnsemblBacteria" id="AAK42047">
        <property type="protein sequence ID" value="AAK42047"/>
        <property type="gene ID" value="SSO1859"/>
    </dbReference>
    <dbReference type="GeneID" id="1453374"/>
    <dbReference type="KEGG" id="sso:SSO1859"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1909"/>
    <dbReference type="eggNOG" id="arCOG01331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042266_4_1_2"/>
    <dbReference type="InParanoid" id="Q97X95"/>
    <dbReference type="PhylomeDB" id="Q97X95"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07338">
        <property type="entry name" value="M48B_HtpX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00188">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050083">
        <property type="entry name" value="HtpX_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022919">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221">
        <property type="entry name" value="PROTEASE HTPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221:SF2">
        <property type="entry name" value="PROTEASE HTPX HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000138930" description="Protease HtpX homolog 1">
        <location>
            <begin position="1"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00188"/>
        </source>
    </evidence>
    <sequence length="311" mass="34985" checksum="7FCDCC0BFC8759F2" modified="2001-10-01" version="1">MDVRQRLISSMVISLGLTIISEGIVLIGIASLLHISLFFIFPALVIFWLFQWIISPYVVGRGGYEVSPNDPQYGWLYNLVRRIAEESKIKPPRVFVIDAPYPNAFAYGNRLGGMRVGITLPLLNILDVDELTAVIAHEVGHIKHRDVEIGMTIGLIPTVLGYISTLLMNFGYLALFLAADEIELLFAIAALAIGFVIFVVTFILQIFVLWFNRLRESYADYNSFLVLGEGSKALATALAKIEIYMQNIRIDPFTGIIVTAPPVKVEEKDPHLLVEQWLRTKVSAFKDILSTHPYPARRAQMIYRLIYGSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XC2</accession>
    <name>Q97XC2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Partial transposase ISC1359</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1822</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90345">
        <property type="entry name" value="D90345"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XC2"/>
    <dbReference type="PaxDb" id="273057-SSO1822"/>
    <dbReference type="EnsemblBacteria" id="AAK42019">
        <property type="protein sequence ID" value="AAK42019"/>
        <property type="gene ID" value="SSO1822"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1822"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1881"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067987_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XC2"/>
    <dbReference type="PhylomeDB" id="Q97XC2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033541">
        <property type="entry name" value="transpos_ISH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="32"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="288" mass="33601" checksum="710410FC42CF6015" modified="2001-10-01" version="1">MRKLNVVQVEDVRDALRKMGRITLRGVRDRRVAVDFHAIPQYHANKSFLSRIKPTKGTSWGLVQAAIFLLGSFLDVIPVTVKNVAEGFKAVMEVIVKELEEDKLRLVMVFADREFAVNEVIRYLLELGLDFVISAKAQMYKKYKGMLQDVDVSFGGVRYTGFLCVRHGSGAYLIILRKEDGKIIAFLVRREMDLYDAIVLAEMYRERWGIENAFRSLEEFRIRTRTCDVRKELVLVLLSYLLLNVWFLIRSWRKVKLWEFSVSLSNLLDREVRVEQERAFREVKTSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XK2</accession>
    <name>Q97XK2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposase ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1731</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90334">
        <property type="entry name" value="C90334"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923688.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XK2"/>
    <dbReference type="STRING" id="273057.SSO1731"/>
    <dbReference type="PaxDb" id="273057-SSO1731"/>
    <dbReference type="EnsemblBacteria" id="AAK41930">
        <property type="protein sequence ID" value="AAK41930"/>
        <property type="gene ID" value="SSO1731"/>
    </dbReference>
    <dbReference type="GeneID" id="1454709"/>
    <dbReference type="KEGG" id="sso:SSO1731"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1774"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066381_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XK2"/>
    <dbReference type="PhylomeDB" id="Q97XK2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="3">
        <location>
            <begin position="194"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="316" mass="35957" checksum="AE7CA659ECB83467" modified="2001-10-01" version="1">MEAPVAGIDVSKDKLVVYFQGKYYEFQNNKQGFEGVKQVLPKGCKVAIESTGIYHINLAKYLGKEYDVRIINPLVLKKFKDIRGKKSDKNDAKKLAELVISMGSEFTTSEAKESTSQWDFITRSIVRVKNRLRRDLILLGYKDSLSKKNLNEVLRGEDNIVLSEVRFLLDELERLEVRKREIEEELEDLVPKDSLIFTIPGIGRTLGCIILARVGDVKRFGNKKRFVAYCGLDPLVESSGKSVVSRGISRRGDAVLRRAFYLAALTAIKVNPIIKRFYEEHKENLKGKKLITACARKLAVITWAVLYYNKPFDASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XK9</accession>
    <name>Q97XK9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Third ORF in transposon ISC1491</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1722</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90333">
        <property type="entry name" value="D90333"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XK9"/>
    <dbReference type="SMR" id="Q97XK9"/>
    <dbReference type="STRING" id="273057.SSO1722"/>
    <dbReference type="PaxDb" id="273057-SSO1722"/>
    <dbReference type="EnsemblBacteria" id="AAK41923">
        <property type="protein sequence ID" value="AAK41923"/>
        <property type="gene ID" value="SSO1722"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1722"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1767"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153635_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XK9"/>
    <dbReference type="PhylomeDB" id="Q97XK9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF17">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="138" mass="15272" checksum="D138F91346AC5524" modified="2001-10-01" version="1">MISAATIYAEFGDVSRFSSSKARAYAGFAPRIKQSGGSESHSGMIRGNKYLRRAFFLVARVARKSERFKEYYDRLVSKWKSATKATCALGGKLACICYHVIKDGVYKGDVKKSFRAPRGKDVNVQDVDVGDVLDSLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XM0</accession>
    <name>Q97XM0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1693</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90330">
        <property type="entry name" value="H90330"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XM0"/>
    <dbReference type="FunCoup" id="Q97XM0">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1693"/>
    <dbReference type="PaxDb" id="273057-SSO1693"/>
    <dbReference type="EnsemblBacteria" id="AAK41903">
        <property type="protein sequence ID" value="AAK41903"/>
        <property type="gene ID" value="SSO1693"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1693"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1745"/>
    <dbReference type="eggNOG" id="arCOG03649">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_20_3_2"/>
    <dbReference type="InParanoid" id="Q97XM0"/>
    <dbReference type="PhylomeDB" id="Q97XM0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="371"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="26"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="462" mass="49427" checksum="1760097908CDB7BA" modified="2001-10-01" version="1">MEKMGKETEKNTQQGLAKGVVGYREVLAQGIASGAPAAATIGTLTGAAAFAYGALPLAVAIALIMVLLDATRLSIIARYVQSAGGIYAFIERGLGKKVAFVASMAYTIYIFAVIIFVYLIMGLMVPTGIEELGINLPSWIWIPLGLVNAIVSVIISYLGIRPSLKFTLIMSIAEITGIIATSILIFSKIPPDINTFTLAYVPPPKISNLGLGVSFGILAFTGYETVSVLGEEAKDPRNNIAKGVFTAALLLGITYIVGSEAFTVGWGVNNMSTYFNYLAPGLILAKTFGGTILLIILTALLINSNLACATGFTNCVTRVMYAMSVDKLLPSKLSSIHPKRRTPHIASLFTFIFTALYFIIMSLIFSPSNIFIATGITTTFGFLIAIFVVNISMLIVVKKNNALNIGNILIVAIVEAIIGYVFYATVITSAINVPVLIGVLTLVLWVVGGIIYLTLKRFFTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XT1</accession>
    <name>Q97XT1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1626</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90323">
        <property type="entry name" value="B90323"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923528.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XT1"/>
    <dbReference type="STRING" id="273057.SSO1626"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1626"/>
    <dbReference type="EnsemblBacteria" id="AAK41841">
        <property type="protein sequence ID" value="AAK41841"/>
        <property type="gene ID" value="SSO1626"/>
    </dbReference>
    <dbReference type="GeneID" id="1454620"/>
    <dbReference type="KEGG" id="sso:SSO1626"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1670"/>
    <dbReference type="eggNOG" id="arCOG05431">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_782162_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XT1"/>
    <dbReference type="PhylomeDB" id="Q97XT1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="207"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="354" mass="40645" checksum="D184CB73B4B3FDD3" modified="2001-10-01" version="1">MFTIALRVLWNGGVARIAVEQARNAKAKLLVYRESFYNYDLSGIYVEFLRRKGEKGFLTPLFRKITEIYAKNRGDEATVDLDLIVKASRIIKEPALFHDQFAGITGYLRKVFHGEDYAIYLHETSLTLKGIKYLIPKVMERKVLEGAKIILTNSQWNKRVLEDKGMGAEVLYPGCYTIDEVEEDKENFVLAVSMWDEGRRPEIYGEIAKRIKGRLIMAGSWARRDTMEEFKRKYPEVIVTGRISEDKLRELYKKASLTIRFGFDERGPGMAVLESLCNGTAVIVNDGIGGKEFVIEGENGYVVKDWKEAVDRINEVLENHKLRRSLSINALETGKKYSWRNHAIKLTEIMEKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XW5</accession>
    <name>Q97XW5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Medium-chain-fatty-acid--CoA ligase (AlkK-1)</fullName>
            <ecNumber evidence="2">6.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">alkK-1</name>
        <name evidence="2" type="ordered locus">SSO1595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.-" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90318">
        <property type="entry name" value="E90318"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XW5"/>
    <dbReference type="FunCoup" id="Q97XW5">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1595"/>
    <dbReference type="PaxDb" id="273057-SSO1595"/>
    <dbReference type="EnsemblBacteria" id="AAK41804">
        <property type="protein sequence ID" value="AAK41804"/>
        <property type="gene ID" value="SSO1595"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1595"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1629"/>
    <dbReference type="eggNOG" id="arCOG02623">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035301_1_1_2"/>
    <dbReference type="InParanoid" id="Q97XW5"/>
    <dbReference type="PhylomeDB" id="Q97XW5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028154">
        <property type="entry name" value="AMP-dep_Lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43845">
        <property type="entry name" value="BLR5969 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43845:SF1">
        <property type="entry name" value="BLR5969 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14535">
        <property type="entry name" value="AMP-binding_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent ligase C-terminal" evidence="1">
        <location>
            <begin position="380"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="475" mass="55389" checksum="239A3ECF83797130" modified="2001-10-01" version="1">MMSSVKNEYDAIHRELREQGFIRTDYPPNPSEVLWNKKIMTMKREEIDKLKTFRLRRIIKWAWENIEFYRRFWKSKNFEPDMIKDWRDIAKIPILRKDEIRKDLQQNPPFGTIFHPELANHIRFVGATSGSTGLPTFQGWGALEMDYFQEAQARYLWTFAEVKPTKVYANYLNMSGFYSWGPPVVETAMWRCGATVIAGGGETYFSWKNRHNLIFKLWKVDVFATTPWLHRLVGEEAKIEGWETPFKVLLLHGGAAAENTKKKLFKVHPNAELAISVWGTTDGHMAIEVPSLEGQLVVWEDMEIFDIVDPKTDEPVSGGERGELIATLLNHFTMPLIRYSLGDYVKNDFLTDPDPKYGITHMRFAEPIPGRVEWMFFVKGKLLLPIYVEDAVNEIPDTTGMFNIFIYDNSMDKLKIRIETRREQVNSTYDKQAREILASRIGINPDDVEIEWVKPGNTIWTGYKLQVFVDQRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XY9</accession>
    <name>Q97XY9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1160</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO8382</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK67522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XY9"/>
    <dbReference type="STRING" id="273057.SSO8382"/>
    <dbReference type="PaxDb" id="273057-SSO8382"/>
    <dbReference type="EnsemblBacteria" id="AAK67522">
        <property type="protein sequence ID" value="AAK67522"/>
        <property type="gene ID" value="SSO8382"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8382"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1603"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2968627_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XY9"/>
    <dbReference type="PhylomeDB" id="Q97XY9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="9"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK67522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="58" mass="6902" checksum="B50E3722EB35C5F3" modified="2001-10-01" version="1">MFKTSSQTVRNYAEKKEVAEKLLERVKQLSLEMLKAVKKVNISIDWTTIKYQTNREVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y33</accession>
    <name>Q97Y33_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO8276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90310">
        <property type="entry name" value="C90310"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y33"/>
    <dbReference type="STRING" id="273057.SSO8276"/>
    <dbReference type="PaxDb" id="273057-SSO8276"/>
    <dbReference type="EnsemblBacteria" id="AAK41738">
        <property type="protein sequence ID" value="AAK41738"/>
        <property type="gene ID" value="SSO8276"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8276"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1556"/>
    <dbReference type="eggNOG" id="arCOG02129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2353342_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y33"/>
    <dbReference type="PhylomeDB" id="Q97Y33"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="4"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="96" mass="11437" checksum="361911D88FD7EDAA" modified="2001-10-01" version="1">MWVRVYMVMDNYSPHKTKGTLEVCRRKGIHPVFTPPYSPELNMAEAVFKSLKNYMSNKIFYTIEDVKNCIKQFFEENKYRFNLNAITYLGLDKIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y38</accession>
    <name>Q97Y38_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR type III-associated protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1512</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90309">
        <property type="entry name" value="F90309"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990109.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y38"/>
    <dbReference type="STRING" id="273057.SSO1512"/>
    <dbReference type="PaxDb" id="273057-SSO1512"/>
    <dbReference type="EnsemblBacteria" id="AAK41733">
        <property type="protein sequence ID" value="AAK41733"/>
        <property type="gene ID" value="SSO1512"/>
    </dbReference>
    <dbReference type="GeneID" id="1454512"/>
    <dbReference type="KEGG" id="sso:SSO1512"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1551"/>
    <dbReference type="eggNOG" id="arCOG03891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_580909_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y38"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09657">
        <property type="entry name" value="Cmr1_III-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007522">
        <property type="entry name" value="CRISPR-assoc_prot_TM1795"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01894">
        <property type="entry name" value="cas_TM1795_cmr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="470" mass="54121" checksum="E219A91C68664FDD" modified="2001-10-01" version="1">MVNLDELKQEVEELIRSKRVARINFVGVTPWWGRDHRGYTSDHVDEDGIVGKLRWFLRTVYNRFCVKNLNSYDEADSYVFEYLGSGNRKSLYIFKVSDSRSIPNKKYEKLNRVNVIIRGKEQENLIPRDMNFTLEIFRSNDDPKYDEIVVGGVLITIAFLGIGFSPNRGFGRFIPAECNDTVAKDICSSVIEGKIIDAFNKFYEKFREIEKGCNRINGWEESHVPLAPLTESNGPDRIQIIEACNKNDIIDVLNVIHKSVLKSSFKSNIRDPAPHIHTWIYGLPRNASITVSTTLTDIRDVEIKEKVGENNVRKEIEDLFENLKKSGNIKIIERYDKRANKQVHYLRSPSGYYKLEGSKLTDVKRESMFIISPIRTSNNSYTISILPFLSLKDNEEALDNLVHIGIHDGRTIHIVPLKEIISMNKADSYLFDKEKELAINNKDLSFPDNVSKLIQVYTTALKDNIMKECR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y77</accession>
    <name>Q97Y77_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO1462</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90304">
        <property type="entry name" value="C90304"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989149.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y77"/>
    <dbReference type="STRING" id="273057.SSO1462"/>
    <dbReference type="PaxDb" id="273057-SSO1462"/>
    <dbReference type="EnsemblBacteria" id="AAK41690">
        <property type="protein sequence ID" value="AAK41690"/>
        <property type="gene ID" value="SSO1462"/>
    </dbReference>
    <dbReference type="GeneID" id="1454469"/>
    <dbReference type="KEGG" id="sso:SSO1462"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1494"/>
    <dbReference type="eggNOG" id="arCOG05331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2204234_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y77"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp_TsuA/YedE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574:SF1">
        <property type="entry name" value="SULPHUR TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="107" mass="12018" checksum="9471937E9819A836" modified="2001-10-01" version="1">MPKASLPYYSEVLIGVRKCMNVLVTKSLVEVRETEDYSLLFFLGAFFGAFLVLFEARIANCCTFGHILSGNLQMVISSFAFLIAVMLGVWITLSLVLRLRINSIGYR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-05-16" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y84</accession>
    <name>CAS1B_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRISPR-associated endonuclease Cas1 2</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cas1-2</name>
        <name type="ordered locus">SSO1450</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2009" name="FEBS Lett." volume="583" first="1928" last="1932">
            <title>SSO1450--a CAS1 protein from Sulfolobus solfataricus P2 with high affinity for RNA and DNA.</title>
            <authorList>
                <person name="Han D."/>
                <person name="Lehmann K."/>
                <person name="Krauss G."/>
            </authorList>
            <dbReference type="PubMed" id="19427858"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2009.04.047"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>DNA-BINDING</scope>
        <scope>RNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2015" name="Elife" volume="4" first="0" last="0">
            <title>Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition.</title>
            <authorList>
                <person name="Rollie C."/>
                <person name="Schneider S."/>
                <person name="Brinkmann A.S."/>
                <person name="Bolt E.L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="26284603"/>
            <dbReference type="DOI" id="10.7554/eLife.08716"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>MUTAGENESIS OF GLU-142</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="function">
        <text evidence="2 3">In vitro catalyzes a concerted transesterification reaction on branched DNA, as would be expected during integration of protospacers into the CRISPR leader sequence; Cas2 is not required in vitro. This reaction requires a 3'-OH group at the branch point (PubMed:26284603). Binds ss- and dsDNA and ss- and dsRNA with approximately equal affinity. May be able to anneal complementary DNA strands (PubMed:19427858).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Homodimer, forms a heterotetramer with a Cas2 homodimer (By similarity). Forms oligomers, probably binds nucleic acids as a homodimer (PubMed:19427858).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated endonuclease Cas1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90303">
        <property type="entry name" value="B90303"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923412.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y84"/>
    <dbReference type="SMR" id="Q97Y84"/>
    <dbReference type="STRING" id="273057.SSO1450"/>
    <dbReference type="PaxDb" id="273057-SSO1450"/>
    <dbReference type="EnsemblBacteria" id="AAK41681">
        <property type="protein sequence ID" value="AAK41681"/>
        <property type="gene ID" value="SSO1450"/>
    </dbReference>
    <dbReference type="GeneID" id="1454460"/>
    <dbReference type="KEGG" id="sso:SSO1450"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1480"/>
    <dbReference type="eggNOG" id="arCOG01452">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052779_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y84"/>
    <dbReference type="PhylomeDB" id="Q97Y84"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099048">
        <property type="term" value="P:CRISPR-cas system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09634">
        <property type="entry name" value="Cas1_I-II-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.920">
        <property type="entry name" value="CRISPR-associated endonuclease Cas1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.20">
        <property type="entry name" value="CRISPR-associated endonuclease Cas1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01470">
        <property type="entry name" value="Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050646">
        <property type="entry name" value="Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002729">
        <property type="entry name" value="CRISPR-assoc_Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042206">
        <property type="entry name" value="CRISPR-assoc_Cas1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042211">
        <property type="entry name" value="CRISPR-assoc_Cas1_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00287">
        <property type="entry name" value="cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34353">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDONUCLEASE CAS1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34353:SF2">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDONUCLEASE CAS1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01867">
        <property type="entry name" value="Cas_Cas1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000417111" description="CRISPR-associated endonuclease Cas1 2">
        <location>
            <begin position="1"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 4">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="No longer catalyzes a transesterification reaction on branched DNA." evidence="3">
        <original>E</original>
        <variation>A</variation>
        <location>
            <position position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="19427858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="26284603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="26284603"/>
        </source>
    </evidence>
    <sequence length="307" mass="34895" checksum="A179E60F2BBB6410" modified="2001-10-01" version="1">MISVRTLVISEYGAYVYVKKNMLVIKKGDKKVEISPSEVDEILITVSCSISTSALSLALTHGISVMFLNSRETPWGILLPSIVTETVKTKKAQYEAIVVRKDNRYGEEIISSKIYNQSVHLKYWARVTGTKNDYKELLDKDEPAAARVYWQNISQLLPKDIGFDGRDVDGTDQFNMALNYSYAILYNTIFKYLVIAGLDPYLGFIHKDRPGNESLVYDFSEMFKPYIDFLLVRALRSGFRLKVKGGLIEENSRGDLAKLIRKGMEENVKEESDHNPKTLIQAIRAHAVKLASSIREGKEYRGFKLVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YB9</accession>
    <name>Q97YB9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90298">
        <property type="entry name" value="E90298"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923381.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YB9"/>
    <dbReference type="PaxDb" id="273057-SSO1409"/>
    <dbReference type="DNASU" id="1454423"/>
    <dbReference type="EnsemblBacteria" id="AAK41644">
        <property type="protein sequence ID" value="AAK41644"/>
        <property type="gene ID" value="SSO1409"/>
    </dbReference>
    <dbReference type="GeneID" id="1454423"/>
    <dbReference type="KEGG" id="sso:SSO1409"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1434"/>
    <dbReference type="eggNOG" id="arCOG07237">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1136104_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YB9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18708">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="28241" checksum="410FFC4E45586ACD" modified="2001-10-01" version="1">MHGNADVKFGNESVFDLARGSSTNDELLNICMIRSGFKKFEEEKEIQNTDGFFIADTSVYIKLGNRLGYLTKDRLLASKSVYNELSERTKLTQMGKEIIVFYLGMYSYRHLHKSPPVSEYNKSGDIPLIEETRKIKENIPEKVTLITADRQLKQRARTLGVNVIFLNTLKSDSGNMSELLLCASNRREYMEKYDYARRDLTITINDKEVIKIESDPTKEGFSRIKTLNKEFNYAKLIEKLYEMI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YC5</accession>
    <name>Y1401_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Putative CRISPR-associated protein SSO1401</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO1401</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="J. Biol. Chem." volume="286" first="21643" last="21656">
            <title>Structural and functional characterization of an archaeal clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for antiviral defense (CASCADE).</title>
            <authorList>
                <person name="Lintner N.G."/>
                <person name="Kerou M."/>
                <person name="Brumfield S.K."/>
                <person name="Graham S."/>
                <person name="Liu H."/>
                <person name="Naismith J.H."/>
                <person name="Sdano M."/>
                <person name="Peng N."/>
                <person name="She Q."/>
                <person name="Copie V."/>
                <person name="Young M.J."/>
                <person name="White M.F."/>
                <person name="Lawrence C.M."/>
            </authorList>
            <dbReference type="PubMed" id="21507944"/>
            <dbReference type="DOI" id="10.1074/jbc.M111.238485"/>
        </citation>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35091 / DSM 1616 / JCM 8930 / NBRC 15331 / P1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Sometimes seen associated with the aCascade ribonucleoprotein complex, minimally composed of Csa2 and Cas5a, which binds crRNA. Other probable components of aCascade in strain P1 are Cas6 and Csa5, while SSO1399, Cas5b (SSO1400) and SSO1401 have sometimes been seen weakly associated. The Csa2-Cas5a-crRNA complex also binds target DNA homologous to crRNA, probably forming an R-loop. Purified aCascade forms a filament about 6 nm in width.</text>
    </comment>
    <comment type="miscellaneous">
        <text>The aCascade complex was purified from strain P1.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90297">
        <property type="entry name" value="E90297"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988397.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YC5"/>
    <dbReference type="STRING" id="273057.SSO1401"/>
    <dbReference type="PaxDb" id="273057-SSO1401"/>
    <dbReference type="EnsemblBacteria" id="AAK41636">
        <property type="protein sequence ID" value="AAK41636"/>
        <property type="gene ID" value="SSO1401"/>
    </dbReference>
    <dbReference type="GeneID" id="1454415"/>
    <dbReference type="KEGG" id="sso:SSO1401"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1417"/>
    <dbReference type="eggNOG" id="arCOG07297">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_859487_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YC5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000417887" description="Putative CRISPR-associated protein SSO1401">
        <location>
            <begin position="1"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="21507944"/>
        </source>
    </evidence>
    <sequence length="323" mass="37140" checksum="9599D14AC93D618F" modified="2001-10-01" version="1">MVFKINLPPEGLFARSLVISELLGLNNVGISIDNDVVNIERKEEFKDNLKNELSQFKLVFNKLKTYKKNYANEVNLAEKVFNGDVEEFFKEDKEEYFLPLIFPEIMEAEKWFGGWSGSGKGSKRTIGVNRQSFILSLLSLGKYQLVSYRAAKEQVTVLALVDTTVMSDMCKPSKKKELRVQSNLIAQLSHISRLLIFSTILDEQGCQEVLLLKEGTHRAEIYERNSHTSLRPLIRFWTLVDDDSVERRITSLANQSPDSLNKVSNYIFEGIRGTLSPVEVAYMIARETYLKEEQSPLTSWDVKKIREALERMRVEMEEVYSST</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-09-05" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YD5</accession>
    <name>CSX1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR system endoribonuclease Csx1</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">csx1</name>
        <name type="ordered locus">SSO1389</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2018" name="Nature" volume="562" first="277" last="280">
            <title>Ring nucleases deactivate type III CRISPR ribonucleases by degrading cyclic oligoadenylate.</title>
            <authorList>
                <person name="Athukoralage J.S."/>
                <person name="Rouillon C."/>
                <person name="Graham S."/>
                <person name="Grueschow S."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="30232454"/>
            <dbReference type="DOI" id="10.1038/s41586-018-0557-5"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="submission" date="2006-08" db="PDB data bank">
            <title>The crystal structure of a conserved hypothetical protein from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Tan K."/>
                <person name="Skarina T."/>
                <person name="Onopriyenko O."/>
                <person name="Savchenko A."/>
                <person name="Edwards A."/>
                <person name="Joachimiak A."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). The type III Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Activated by cyclic tetraadenylate (cA4) to endonucleolytically degrade linear RNA; when cA4 levels decrease the RNase activity of Csx1 is abrogated.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The RNase activity is stimulated by cA4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR-associated Csx1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90296">
        <property type="entry name" value="C90296"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991619.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2I71">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A/B=1-377"/>
    </dbReference>
    <dbReference type="PDBsum" id="2I71"/>
    <dbReference type="AlphaFoldDB" id="Q97YD5"/>
    <dbReference type="SMR" id="Q97YD5"/>
    <dbReference type="STRING" id="273057.SSO1389"/>
    <dbReference type="PaxDb" id="273057-SSO1389"/>
    <dbReference type="EnsemblBacteria" id="AAK41626">
        <property type="protein sequence ID" value="AAK41626"/>
        <property type="gene ID" value="SSO1389"/>
    </dbReference>
    <dbReference type="GeneID" id="1454405"/>
    <dbReference type="GeneID" id="44130246"/>
    <dbReference type="KEGG" id="sso:SSO1389"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1397"/>
    <dbReference type="eggNOG" id="arCOG03433">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_695624_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YD5"/>
    <dbReference type="PhylomeDB" id="Q97YD5"/>
    <dbReference type="EvolutionaryTrace" id="Q97YD5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09660">
        <property type="entry name" value="Csx1_III-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10640">
        <property type="entry name" value="SSO1389-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3740.10">
        <property type="entry name" value="SSO1389-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027419">
        <property type="entry name" value="CRISPR-assoc_Csx1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013383">
        <property type="entry name" value="CRISPR-assoc_prot_DxTHG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052875">
        <property type="entry name" value="CRISPR_assoc_ribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010171">
        <property type="entry name" value="CRISPR_Csx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019016">
        <property type="entry name" value="Csx1-like_HEPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053857">
        <property type="entry name" value="Csx1_CARF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01897">
        <property type="entry name" value="cas_MJ1666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02549">
        <property type="entry name" value="CRISPR_DxTHG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37169:SF1">
        <property type="entry name" value="CRISPR SYSTEM ENDORIBONUCLEASE CSX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37169">
        <property type="entry name" value="CRISPR SYSTEM ENDORIBONUCLEASE CSX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22230">
        <property type="entry name" value="Csx1_CARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09455">
        <property type="entry name" value="Csx1_HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160980">
        <property type="entry name" value="SSO1389-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418436" description="CRISPR system endoribonuclease Csx1">
        <location>
            <begin position="1"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="2"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="18"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="25"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="43"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="51"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="95"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="156"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="172"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="177"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="202"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="228"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="252"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="260"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="267"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="280"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="301"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="326"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="341"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="345"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="350"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="354"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="361"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="367"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="370"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="30232454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="30232454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="30232454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5">
        <source ref="3"/>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="2I71"/>
        </source>
    </evidence>
    <sequence length="377" mass="42929" checksum="B309EE3D233BC286" modified="2001-10-01" version="1">MASIVFSTIGNPKGYQKVTYEIDGEKFESNVSVLALRDLLKVDKTVVILGISVADVYNCKYADYRSCKECIIQNSKNDLGISESYVVAPNVYQKFKGKPDHYFTYIYYHSLRILEKEGINEVFIDTTHGINYMGVLAKEAIQLAVSAYAAKSEKEVKVSLYNSDPVGKDVSDTVKLHEIEAIKISPLSGLKYVTYQILNKDKNFFNKIFSDSVNAIPRFATALDNGLFIYLSEKDSSLHLKRLEDDLSKDPLLTPSENEINVVYKDMKYALSHALFYVISRFSGNVDLDTLRHYAETYADKVTRAIIENEVDKIEKYQMGSERKLLGEYMKVEGKGFDKRILYAHGGLPYAGTYVYKEKDKVYVTYGDKIDEIERQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YE0</accession>
    <name>Q97YE0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO7893</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90295">
        <property type="entry name" value="F90295"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YE0"/>
    <dbReference type="STRING" id="273057.SSO7893"/>
    <dbReference type="PaxDb" id="273057-SSO7893"/>
    <dbReference type="EnsemblBacteria" id="AAK41621">
        <property type="protein sequence ID" value="AAK41621"/>
        <property type="gene ID" value="SSO7893"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7893"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1393"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166569_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YE0"/>
    <dbReference type="PhylomeDB" id="Q97YE0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="85" mass="9544" checksum="FF89D4EA40424043" modified="2001-10-01" version="1">MEAPVAGIDVSKDKLVVYFQGKYYEFQNNKQGFEGVKQVLPKGCKVAIESTGVYHINLAKYLGKEYDVSAFRKFIALGKGYITRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YE3</accession>
    <name>Q97YE3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PaREP1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1383</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90295">
        <property type="entry name" value="B90295"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991931.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YE3"/>
    <dbReference type="PaxDb" id="273057-SSO1383"/>
    <dbReference type="DNASU" id="1454396"/>
    <dbReference type="EnsemblBacteria" id="AAK41617">
        <property type="protein sequence ID" value="AAK41617"/>
        <property type="gene ID" value="SSO1383"/>
    </dbReference>
    <dbReference type="GeneID" id="1454396"/>
    <dbReference type="KEGG" id="sso:SSO1383"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1388"/>
    <dbReference type="eggNOG" id="arCOG03712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118419_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YE3"/>
    <dbReference type="PhylomeDB" id="Q97YE3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="20243" checksum="81EF8484615E25B0" modified="2001-10-01" version="1">MESQLPKFLKEPEKYSRLRLLEALQELYLSIEMLKEGYSRNSASKLFLSWKALLSSIVVTNFGKIMETKKKEGKEDEIKWYMRIGYSAPTTGLIGIARDLENLGFKGLVNLTTAMLGIHRYAYNGLDEDISPFHSRSEAIISIKGLIKAEVEIVGSMKFGDEEKELFEKIKRELNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YG2</accession>
    <name>Q97YG2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transport membrane protein (Permease)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1361</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90292">
        <property type="entry name" value="E90292"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923353.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YG2"/>
    <dbReference type="FunCoup" id="Q97YG2">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1361"/>
    <dbReference type="PaxDb" id="273057-SSO1361"/>
    <dbReference type="EnsemblBacteria" id="AAK41596">
        <property type="protein sequence ID" value="AAK41596"/>
        <property type="gene ID" value="SSO1361"/>
    </dbReference>
    <dbReference type="GeneID" id="1454375"/>
    <dbReference type="KEGG" id="sso:SSO1361"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1367"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_762112_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YG2"/>
    <dbReference type="PhylomeDB" id="Q97YG2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010645">
        <property type="entry name" value="MFS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23537">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23537:SF1">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="4"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="374" mass="41026" checksum="5B3A8183B71E8F90" modified="2001-10-01" version="1">MLRNIIVGWIGTFLQLFLRLSWGVISLPIALLFHLNSIQIGLVATAFYIGYVLSSIPWGLIIDRIGPSRAVEYASIILVGMNLLLFLYLTDYTILLIAYLIEGLITASIFPSAMKIVAVSYSHSSKFTFYVALVESAGPITIITLGIIASFLLHLWRFIYLAMAVSFGLIAILSHFSRISVSGTEIKRSFRVILNRKITMATLVRLGELWSTWGTTTWIFPMLVLYRGISPTLSALFLLLFGVGQLVGILSVEKLVERFGDSNVILINLIGFIILTFLIIFSNDIDILPEAFLLGIFSFSYRPPTDSLIMKIAGESSAGTSIGFANAVSQIGTMIAPSFVGLVLYLTHSFSISMLALDAGCVISIVSLLSLKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YI3</accession>
    <name>Q97YI3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetyl-CoA synthetase (Acetate-CoA ligase) carboxy-end. (AcsA-2)</fullName>
            <ecNumber evidence="7">6.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">acsA-2</name>
        <name evidence="7" type="ordered locus">SSO1340</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99290">
        <property type="entry name" value="A99290"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YI3"/>
    <dbReference type="FunCoup" id="Q97YI3">
        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1340"/>
    <dbReference type="PaxDb" id="273057-SSO1340"/>
    <dbReference type="EnsemblBacteria" id="AAK41576">
        <property type="protein sequence ID" value="AAK41576"/>
        <property type="gene ID" value="SSO1340"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1340"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1344"/>
    <dbReference type="eggNOG" id="arCOG04201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_17_0_2"/>
    <dbReference type="InParanoid" id="Q97YI3"/>
    <dbReference type="PhylomeDB" id="Q97YI3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000005">
        <property type="entry name" value="Acyl-coenzyme A synthetase ACSM5, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051087">
        <property type="entry name" value="Mitochondrial_ACSM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605:SF10">
        <property type="entry name" value="ACYL-COA SYNTHETASE MEDIUM CHAIN FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605">
        <property type="entry name" value="ACYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="5"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="168"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="260" mass="29551" checksum="D71B9D7CAE60A154" modified="2001-10-01" version="1">MRFHPPTALRMIRKEVSSPRRDYDLRLRAVSSAGEAVGGDLIEWAMKELSPNVNEFYGCTEANLVTVNNSMWRKIGSVGKPTPGHEIAVIDEKGNEVINQVGEIAVRIEDPVLFLGYYKNPEATAKKFRGHWFLMGDLGLMDQNGYIWFKGRGDDVIKVSGYRLGPEEIESIILQHPAVQEVAVIGKPDRLRGNVIKAFIVLKDGYSPSDSLVEEIQQFVKSRLALYAYPREIEFVKELPRTETGKLKRFELRKREEEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YL9</accession>
    <name>Q97YL9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mandelate racemase /muconate lactonizing enzyme related protein (MR/MLE)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1303</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90285">
        <property type="entry name" value="E90285"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YL9"/>
    <dbReference type="FunCoup" id="Q97YL9">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1303"/>
    <dbReference type="PaxDb" id="273057-SSO1303"/>
    <dbReference type="EnsemblBacteria" id="AAK41540">
        <property type="protein sequence ID" value="AAK41540"/>
        <property type="gene ID" value="SSO1303"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1303"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1304"/>
    <dbReference type="eggNOG" id="arCOG01168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_1_0_2"/>
    <dbReference type="InParanoid" id="Q97YL9"/>
    <dbReference type="PhylomeDB" id="Q97YL9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050032">
        <property type="term" value="F:L-rhamnonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03327">
        <property type="entry name" value="MR_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023444">
        <property type="entry name" value="L-Rhamnon_dehydrat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046945">
        <property type="entry name" value="RHMD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794">
        <property type="entry name" value="ENOLASE SUPERFAMILY, MANDELATE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794:SF58">
        <property type="entry name" value="MITOCHONDRIAL ENOLASE SUPERFAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00179">
        <property type="entry name" value="mandelate_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mandelate racemase/muconate lactonizing enzyme C-terminal" evidence="4">
        <location>
            <begin position="168"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="383" mass="43767" checksum="B3EC9A0BA152A390" modified="2001-10-01" version="1">MIKGCSDKCYSMTAKIDKIYVVTTERISRQNFPYVKPTDYYREYLGSLSPFQSAFMDKVCFIRMDLDDNVTYSILETSESVCNFVMSNLADLVRGLPVSRINMVWDLLYRYTLPIGRSGIAIHAISVINLLMYDAYAKLLGVPVYELLGGKARDKIRAYASHLHPTSKDELEKEARSYVEEGYAAMKMRFCCGPSDPLGVDKNVELVKVIRDSVGYSVDLAGDAWMSWNLNFALKMARRLEKYELSWIEEPLLPDDFESYSYLSKRTDIPISAGEHHYHVYDFKRLLDSGVRILQPDAVWVGGITPMKRITGLAEAYGGVVIPHTSNIYNLHFIISEPESVSPMAEYLTKYKWLEDRMINPPKPIKGYFELGEEKGFGLKYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YM3</accession>
    <name>Q97YM3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">First ORF in transposon ISC1160</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO7621</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK67516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YM3"/>
    <dbReference type="PaxDb" id="273057-SSO7621"/>
    <dbReference type="EnsemblBacteria" id="AAK67516">
        <property type="protein sequence ID" value="AAK67516"/>
        <property type="gene ID" value="SSO7621"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7621"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1300"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2476184_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YM3"/>
    <dbReference type="PhylomeDB" id="Q97YM3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025471">
        <property type="entry name" value="DUF4322"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14210">
        <property type="entry name" value="DUF4322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4322" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK67516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="87" mass="10203" checksum="7A553A898D1659CC" modified="2001-10-01" version="1">MIDFKGRKGKEQAKPSSQPWNDSIENKSKMFKTSSQTVRNYAEKEEVAEKLPKRVKQLSLEMLKAVKKVNISIDWTTIKYQTNREVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YP5</accession>
    <name>Q97YP5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oligo/dipeptide transport, ATP binding protein. carboxy-end. (DppF-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">dppF-2</name>
        <name evidence="5" type="ordered locus">SSO1279</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90282">
        <property type="entry name" value="E90282"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YP5"/>
    <dbReference type="STRING" id="273057.SSO1279"/>
    <dbReference type="PaxDb" id="273057-SSO1279"/>
    <dbReference type="EnsemblBacteria" id="AAK41516">
        <property type="protein sequence ID" value="AAK41516"/>
        <property type="gene ID" value="SSO1279"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1279"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1277"/>
    <dbReference type="eggNOG" id="arCOG00184">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="InParanoid" id="Q97YP5"/>
    <dbReference type="PhylomeDB" id="Q97YP5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067:SF3">
        <property type="entry name" value="MALTOSE ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067">
        <property type="entry name" value="OLIGOPEPTIDE/DIPEPTIDE ABC TRANSPORTER, ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Oligopeptide/dipeptide ABC transporter C-terminal" evidence="4">
        <location>
            <begin position="17"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="103" mass="11592" checksum="A5ED94B4C77F38EF" modified="2001-10-01" version="1">MVKHHCRIGVMYLGKVVEIGTPEEVISEPLHPYTQALLQAVPVPDPDIGIKEPNIKGEIQSPLELPKGCVFYSRCPFAKDICKEKPPEIKRIKGDHYVACHLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YQ8</accession>
    <name>Q97YQ8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90280">
        <property type="entry name" value="H90280"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YQ8"/>
    <dbReference type="STRING" id="273057.SSO1265"/>
    <dbReference type="PaxDb" id="273057-SSO1265"/>
    <dbReference type="EnsemblBacteria" id="AAK41503">
        <property type="protein sequence ID" value="AAK41503"/>
        <property type="gene ID" value="SSO1265"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1265"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1262"/>
    <dbReference type="eggNOG" id="arCOG07731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142043_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YQ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="156" mass="18474" checksum="CA4A2E4F901C6764" modified="2001-10-01" version="1">MMPSRSFQIFRLIFKERYRQPTLELVIPTMLVSNVFISAFYARGVFNYYGLVLAFVPLISVSETLTFALALRNIIFVTGDHIYRGSIISFMMMAIKRISLFFFTYFCDVILPYLFWLFTTELYIFLSNIPVPQYLVLIYTVGYFFTENIVLYFVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YS1</accession>
    <name>Q97YS1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO7486</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90278">
        <property type="entry name" value="H90278"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YS1"/>
    <dbReference type="STRING" id="273057.SSO7486"/>
    <dbReference type="PaxDb" id="273057-SSO7486"/>
    <dbReference type="EnsemblBacteria" id="AAK41487">
        <property type="protein sequence ID" value="AAK41487"/>
        <property type="gene ID" value="SSO7486"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7486"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1241"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197996_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YS1"/>
    <dbReference type="PhylomeDB" id="Q97YS1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="67" mass="7399" checksum="CB9217DBE20B8C32" modified="2001-10-01" version="1">MGELKSSARVTEGGRLVPVGEFPQGEYLVEYLGVPIKLLVVDDYKGLGKRYFFSTNVNDTSEDIITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YS9</accession>
    <name>Q97YS9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90277">
        <property type="entry name" value="D90277"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992601.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YS9"/>
    <dbReference type="STRING" id="273057.SSO1229"/>
    <dbReference type="PaxDb" id="273057-SSO1229"/>
    <dbReference type="EnsemblBacteria" id="AAK41475">
        <property type="protein sequence ID" value="AAK41475"/>
        <property type="gene ID" value="SSO1229"/>
    </dbReference>
    <dbReference type="GeneID" id="1454254"/>
    <dbReference type="KEGG" id="sso:SSO1229"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1229"/>
    <dbReference type="eggNOG" id="arCOG08363">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1792215_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YS9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="144" mass="16779" checksum="28B1A4727B45BADA" modified="2001-10-01" version="1">MSIEAVPIVFVPEEGTVWLPAVLPTNIAVKEAVEMLKSLTVNVLVWEKKGKELRLVNYFTGQVLDPNAKVRDVIKPYDVFWLIWWPPREEFWKPENQNDEIFRIIKETEDAVKSAPRSPSVLFADEIEKYSIVRRLEREGKLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YW7</accession>
    <name>Q97YW7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Isochorismatase related protein (EntB-like1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">entB-like1</name>
        <name evidence="3" type="ordered locus">SSO1184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99272">
        <property type="entry name" value="B99272"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988682.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YW7"/>
    <dbReference type="FunCoup" id="Q97YW7">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1184"/>
    <dbReference type="PaxDb" id="273057-SSO1184"/>
    <dbReference type="EnsemblBacteria" id="AAK41433">
        <property type="protein sequence ID" value="AAK41433"/>
        <property type="gene ID" value="SSO1184"/>
    </dbReference>
    <dbReference type="GeneID" id="1454212"/>
    <dbReference type="KEGG" id="sso:SSO1184"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1181"/>
    <dbReference type="eggNOG" id="arCOG01943">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068979_8_3_2"/>
    <dbReference type="InParanoid" id="Q97YW7"/>
    <dbReference type="PhylomeDB" id="Q97YW7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00431">
        <property type="entry name" value="cysteine_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050272">
        <property type="entry name" value="Isochorismatase-like_hydrls"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540:SF6">
        <property type="entry name" value="ISOCHORISMATASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540">
        <property type="entry name" value="PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="2">
        <location>
            <begin position="18"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="185" mass="21252" checksum="0DDF0814B5940413" modified="2001-10-01" version="1">MSFFNPEEIKRLVNKNNSVLVVWDVQEGLVNSIFNKNEFIAKLKELIESARKYNVPIVYTMITPYPERFQPRIRRSFNPGDIYKEVYPKEGDVILRKNTPSIFVGTNFELMLRNAGIYSIVFTGIATDIGVETSARHAQALGFIPIVAKEAVSSADKEAHERSLLNMQRLMLVLTNKEITELWER</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YX5</accession>
    <name>Q97YX5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">aspB-3</name>
        <name evidence="3" type="ordered locus">SSO1177</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90271">
        <property type="entry name" value="B90271"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991561.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YX5"/>
    <dbReference type="STRING" id="273057.SSO1177"/>
    <dbReference type="PaxDb" id="273057-SSO1177"/>
    <dbReference type="EnsemblBacteria" id="AAK41425">
        <property type="protein sequence ID" value="AAK41425"/>
        <property type="gene ID" value="SSO1177"/>
    </dbReference>
    <dbReference type="GeneID" id="1454204"/>
    <dbReference type="KEGG" id="sso:SSO1177"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1170"/>
    <dbReference type="eggNOG" id="arCOG04333">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_4_2"/>
    <dbReference type="InParanoid" id="Q97YX5"/>
    <dbReference type="PhylomeDB" id="Q97YX5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43510">
        <property type="entry name" value="AMINOTRANSFERASE FUNCTION, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43510:SF1">
        <property type="entry name" value="AMINOTRANSFERASE FUNCTION, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="2">
        <location>
            <begin position="42"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="356" mass="40502" checksum="CDD8F1F710976B54" modified="2001-10-01" version="1">MYPEFCLERWQSLRDWRAKYVLSESGVEPLDLSEIQIPNVKLEYGHTKGLIKLRQLVSSFYPGKKEEDVIITAGGAEANYVTVLSTIEPGDEVIVEMPNYMQIPGLLRGINAKIKYIWLNDNFKLDLNELNEMVSKKTKAIVITNPNNPTGMALSESEIKGIVDIAEDNQVTIIADEVYRGSEHDGNIRPSFIDLYDNAISTNSMSKVYGLPGIRIGWVVANKELVDKMWSIRDYTSISPSILGQEIAYNVLLEKEKYMDRARRIGLNNIRLLERLISDIDVKWVEPNATVLAYLKLNVKNTYDFSARLFEKYSVLVNPGECFEMPGYVRIGLGSTNTEFLNEALSLFIGYLREYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YY8</accession>
    <name>Q97YY8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO1162</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90269">
        <property type="entry name" value="E90269"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991582.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YY8"/>
    <dbReference type="FunCoup" id="Q97YY8">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1162"/>
    <dbReference type="PaxDb" id="273057-SSO1162"/>
    <dbReference type="EnsemblBacteria" id="AAK41412">
        <property type="protein sequence ID" value="AAK41412"/>
        <property type="gene ID" value="SSO1162"/>
    </dbReference>
    <dbReference type="GeneID" id="1454191"/>
    <dbReference type="KEGG" id="sso:SSO1162"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1156"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="InParanoid" id="Q97YY8"/>
    <dbReference type="PhylomeDB" id="Q97YY8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17321">
        <property type="entry name" value="MFS_MMR_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000503">
        <property type="entry name" value="Drug resistance transporter, EmrB/QacA subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000021">
        <property type="entry name" value="Drug resistance transporter, EmrB/QacA subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="259"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="349"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="388"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="433"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="7"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="471" mass="50172" checksum="F15BA8567814728E" modified="2001-10-01" version="1">MNSSNLALLVLVFGTLMSAIDTTIVILAIPTITEDLHADLFIMIWVIILYLLVIAVFTTQLGRLGDIYGRAKFYNLGFAIFTLGSALCGASPNAIYLVVFRGIQGIGAAMMQANAGAIIADIFPPNRRGRAYGYTAIGWNAGATLGIVLGGFITTFVGWRYIFYINVPIGIIALILGLRYIKTLETRKVKLDIAGMGTLLVALSSMVYGAVDIAGYGIRALNMVLISLGLVILLIFFWLEGRHEFPIIDLRVFKGNRVFTFSLLASFLQSTGYLATAFIIIMYLQGIRALSPLNASLLLVPGYVLASLVAPFTGRLSDKIGARMPATIGLSLMIVAVLIYLTLSLNTPLYIVILASTIGGIGSSLFFPANNSAIMANAPKGFYGGASGLARTLANLGSIISYTIAISVSSIVIPRYVAFEVFLGTSNLIGGVASAFLTGLKAAFYVSLAILAIALVLSAIRGKEVRTQVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z71</accession>
    <name>Q97Z71_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90258">
        <property type="entry name" value="C90258"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989894.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z71"/>
    <dbReference type="STRING" id="273057.SSO1059"/>
    <dbReference type="PaxDb" id="273057-SSO1059"/>
    <dbReference type="EnsemblBacteria" id="AAK41322">
        <property type="protein sequence ID" value="AAK41322"/>
        <property type="gene ID" value="SSO1059"/>
    </dbReference>
    <dbReference type="GeneID" id="1454101"/>
    <dbReference type="KEGG" id="sso:SSO1059"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1054"/>
    <dbReference type="eggNOG" id="arCOG07227">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1292084_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z71"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="213" mass="24770" checksum="EB5BBFFB5F821DCF" modified="2001-10-01" version="1">MKEIDLTEEKDANVRKFIVVQNLQSDDTEIVFKVDKDGKNMIEDLCHELEYECEEYEKDGVYSVKIKKSTEVKGSVSDATDFLVPLPPKSVNEKILNPAILTLFIPQIKAVSSIRERIYLLRIKWVISWDTPLTVYSVKLDDNRYIARYYASQKIPLFVVSFGFDFYIMSEGTGSRIKMKEWYKGPFKQLAKGEIEKHLKKAKEVLPEFLIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZF7</accession>
    <name>Q97ZF7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0961</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90247">
        <property type="entry name" value="D90247"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992405.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZF7"/>
    <dbReference type="STRING" id="273057.SSO0961"/>
    <dbReference type="PaxDb" id="273057-SSO0961"/>
    <dbReference type="EnsemblBacteria" id="AAK41235">
        <property type="protein sequence ID" value="AAK41235"/>
        <property type="gene ID" value="SSO0961"/>
    </dbReference>
    <dbReference type="GeneID" id="1455202"/>
    <dbReference type="KEGG" id="sso:SSO0961"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.959"/>
    <dbReference type="eggNOG" id="arCOG04324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156326_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZF7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="106" mass="12036" checksum="80104EB36D691E86" modified="2001-10-01" version="1">MSQPTVKVEFEGKAKIGEMMGNFKAIQLKPEDFSSPLALQMALSRIYSELMNMMNQRQELHYVADVKFTDSMGNPISVGVDFGDKIPPLSKKEVKVKITIEFYDEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZK2</accession>
    <name>OGG1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">8-oxoguanine DNA glycosylase/AP lyase</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1 3">8-oxoguanine DNA glycosylase</fullName>
                <shortName evidence="1">8-oxoG DNA glycosylase</shortName>
                <ecNumber evidence="1">3.2.2.-</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">DNA-(apurinic or apyrimidinic site) lyase</fullName>
                <shortName evidence="1">AP lyase</shortName>
                <ecNumber evidence="1">4.2.99.18</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1 3" type="primary">ogg</name>
        <name type="ordered locus">SSO0904</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2009" name="Structure" volume="17" first="703" last="712">
            <title>Crystal structures of two archaeal 8-oxoguanine DNA glycosylases provide structural insight into guanine/8-oxoguanine distinction.</title>
            <authorList>
                <person name="Faucher F."/>
                <person name="Duclos S."/>
                <person name="Bandaru V."/>
                <person name="Wallace S.S."/>
                <person name="Doublie S."/>
            </authorList>
            <dbReference type="PubMed" id="19446526"/>
            <dbReference type="DOI" id="10.1016/j.str.2009.03.007"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF MUTANT GLN-128</scope>
        <scope>ACTIVE SITE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the excision of an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine = 8-oxoG) from DNA. Also cleaves the DNA backbone at apurinic/apyrimidinic sites (AP sites).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-2 OGG1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="1"/>
    <dbReference type="EC" id="4.2.99.18" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90241">
        <property type="entry name" value="C90241"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992328.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3FHG">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A=1-207"/>
    </dbReference>
    <dbReference type="PDBsum" id="3FHG"/>
    <dbReference type="AlphaFoldDB" id="Q97ZK2"/>
    <dbReference type="SMR" id="Q97ZK2"/>
    <dbReference type="STRING" id="273057.SSO0904"/>
    <dbReference type="PaxDb" id="273057-SSO0904"/>
    <dbReference type="EnsemblBacteria" id="AAK41186">
        <property type="protein sequence ID" value="AAK41186"/>
        <property type="gene ID" value="SSO0904"/>
    </dbReference>
    <dbReference type="GeneID" id="1455153"/>
    <dbReference type="KEGG" id="sso:SSO0904"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.906"/>
    <dbReference type="eggNOG" id="arCOG04357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104937_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZK2"/>
    <dbReference type="PhylomeDB" id="Q97ZK2"/>
    <dbReference type="BRENDA" id="3.2.2.B5">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97ZK2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00241">
        <property type="entry name" value="Ogg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012092">
        <property type="entry name" value="DNA_glyclase/AP_lyase_Ogg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002305">
        <property type="entry name" value="PRK01229.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22175">
        <property type="entry name" value="Ogg-HhH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005954">
        <property type="entry name" value="Thrmst_ogg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0326">Glycosidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000159566" description="8-oxoguanine DNA glycosylase/AP lyase">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="active site" evidence="1 5">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="active site" evidence="1 5">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="site" description="Important for guanine/8-oxoguanine distinction" evidence="1 2">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="3"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="59"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="turn" evidence="7">
        <location>
            <begin position="97"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="112"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="147"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="169"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="19446526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="19446526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="19446526"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="3FHG"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="3FHG"/>
        </source>
    </evidence>
    <sequence length="207" mass="24253" checksum="823B754303F3F950" modified="2001-10-01" version="1">MLRSLVQNPKVRARVLERVDEFRLNNLSNEEVWFRELTLCLLTANSSFISAYQALNCLGQKIYYANEEEIRNILKSCKYRFYNLKAKYIIMAREKVYGRLKEEIKPLADEDQQLARERLLNIKGIGMKEASHFLRNVGYFDLAIIDRHIIDFMRRIGAIGETNVKQLSKSLYISFENILKSIASNLNMSVGILDLFIWYKETNTIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZN2</accession>
    <name>Q97ZN2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SSV1 integrase homolog (Orf D-335)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90236">
        <property type="entry name" value="D90236"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZN2"/>
    <dbReference type="PaxDb" id="273057-SSO0853"/>
    <dbReference type="EnsemblBacteria" id="AAK41147">
        <property type="protein sequence ID" value="AAK41147"/>
        <property type="gene ID" value="SSO0853"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0853"/>
    <dbReference type="eggNOG" id="arCOG07277">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184189_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZN2"/>
    <dbReference type="PhylomeDB" id="Q97ZN2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012922">
        <property type="entry name" value="ORF_D-335"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07935">
        <property type="entry name" value="SSV1_ORF_D-335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ORF D-335-like" evidence="1">
        <location>
            <begin position="16"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="99" mass="11191" checksum="DCE4B88D8451F227" modified="2001-10-01" version="1">MAIIIREGKMTKEKTRYKYGDIIIRERKGRYYVYKLEMINGKVRETYVGPLIDVVESFIKLKEIGGVGISPTTDPPGFEPGTTGSEGRRSILAELRVPL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZQ3</accession>
    <name>RL37A_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL43</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L37Ae</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ribosomal protein L43e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl37ae</name>
        <name type="ordered locus">SSO6418</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family. Putative zinc-binding subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK41029" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41029.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90221">
        <property type="entry name" value="F90221"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991302.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZQ3"/>
    <dbReference type="SMR" id="Q97ZQ3"/>
    <dbReference type="FunCoup" id="Q97ZQ3">
        <property type="interactions" value="185"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO6418"/>
    <dbReference type="PaxDb" id="273057-SSO6418"/>
    <dbReference type="EnsemblBacteria" id="AAK41029">
        <property type="protein sequence ID" value="AAK41029"/>
        <property type="gene ID" value="SSO6418"/>
    </dbReference>
    <dbReference type="GeneID" id="1454996"/>
    <dbReference type="KEGG" id="sso:SSO6418"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.729"/>
    <dbReference type="eggNOG" id="arCOG04208">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141199_2_0_2"/>
    <dbReference type="InParanoid" id="Q97ZQ3"/>
    <dbReference type="PhylomeDB" id="Q97ZQ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003058">
        <property type="entry name" value="PRK03976.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000139854" description="Large ribosomal subunit protein eL43">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="70" mass="8102" checksum="060AECB73D84F47A" modified="2002-03-27" version="2">MGKVTGISGRFGARYGSTLRKKWKEIMEKRYDEHQCPYCKTTGKVIRLASGIWYCKKCNSKWAGLAYTPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZS1</accession>
    <name>Q97ZS1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0508</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90196">
        <property type="entry name" value="E90196"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZS1"/>
    <dbReference type="PaxDb" id="273057-SSO0508"/>
    <dbReference type="EnsemblBacteria" id="AAK40828">
        <property type="protein sequence ID" value="AAK40828"/>
        <property type="gene ID" value="SSO0508"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0508"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.506"/>
    <dbReference type="eggNOG" id="arCOG07847">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2217169_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZS1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="106" mass="12428" checksum="9201155949119678" modified="2001-10-01" version="1">MRTLPTRTSCISAPAFRDIDPHLVVHHQIILCDFFILTYINFTVYRKLLITAETQEIEELTKLIRVYFQLDEVLSFVLEELEDDEVIAELSATKDKIRKAIERMIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZT1</accession>
    <name>Q97ZT1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thermopsin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90195">
        <property type="entry name" value="B90195"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991387.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZT1"/>
    <dbReference type="STRING" id="273057.SSO0497"/>
    <dbReference type="PaxDb" id="273057-SSO0497"/>
    <dbReference type="EnsemblBacteria" id="AAK40817">
        <property type="protein sequence ID" value="AAK40817"/>
        <property type="gene ID" value="SSO0497"/>
    </dbReference>
    <dbReference type="GeneID" id="1455627"/>
    <dbReference type="KEGG" id="sso:SSO0497"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.490"/>
    <dbReference type="eggNOG" id="arCOG07246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_507749_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZT1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="536" mass="59887" checksum="4AEA958D2869F025" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q980C9</accession>
    <name>Q980C9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90182">
        <property type="entry name" value="C90182"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980C9"/>
    <dbReference type="STRING" id="273057.SSO0387"/>
    <dbReference type="PaxDb" id="273057-SSO0387"/>
    <dbReference type="EnsemblBacteria" id="AAK40714">
        <property type="protein sequence ID" value="AAK40714"/>
        <property type="gene ID" value="SSO0387"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0387"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.381"/>
    <dbReference type="eggNOG" id="arCOG04060">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077869_0_0_2"/>
    <dbReference type="InParanoid" id="Q980C9"/>
    <dbReference type="PhylomeDB" id="Q980C9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017271">
        <property type="entry name" value="Tscrpt_reg_HTH_MJ1545_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037724">
        <property type="entry name" value="TF_HTH_MJ1545_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="38"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="246" mass="27750" checksum="A12684B4B394AC54" modified="2001-10-01" version="1">MFITLMAVDELFDSYIIETIGKRISGDIVWSKDISGSLRKWREMFNVSQGELAREMGIKQSVIADYERGRRQAGSEFIKRYVSALISIDARRGYKVVKELAKMFGINFPFIVDMRDFNSPICIDKLIRAVDGVVVNSFVTDKKVYGYVVTDSIRAILSLSGLEFYQVLSMMVNRVVVFTRVSSGRSPMIALKVAPIRPQVVVFHRPVKLDPLALMLSEVEGINIIVSMKPTEEDLIKGLRSLLAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q980D3</accession>
    <name>Q980D3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamine--fructose-6-phosphate transaminase (isomerizing)</fullName>
            <ecNumber evidence="2">2.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">glmS-1</name>
        <name evidence="10" type="ordered locus">SSO0382</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90181">
        <property type="entry name" value="G90181"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988819.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980D3"/>
    <dbReference type="FunCoup" id="Q980D3">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0382"/>
    <dbReference type="MEROPS" id="C44.A08"/>
    <dbReference type="PaxDb" id="273057-SSO0382"/>
    <dbReference type="EnsemblBacteria" id="AAK40710">
        <property type="protein sequence ID" value="AAK40710"/>
        <property type="gene ID" value="SSO0382"/>
    </dbReference>
    <dbReference type="GeneID" id="1455520"/>
    <dbReference type="KEGG" id="sso:SSO0382"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.376"/>
    <dbReference type="eggNOG" id="arCOG00057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_7_0_2"/>
    <dbReference type="InParanoid" id="Q980D3"/>
    <dbReference type="PhylomeDB" id="Q980D3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000143">
        <property type="entry name" value="Glucosamine/fructose-6-phosphate aminotransferase, isomerizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000022">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="8">
        <location>
            <begin position="2"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="9">
        <location>
            <begin position="286"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="9">
        <location>
            <begin position="445"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK40710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="591" mass="65527" checksum="CA690976F9A5A4BF" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q980F6</accession>
    <name>Q980F6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="2">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">SSO0354</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + nicotinate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90178">
        <property type="entry name" value="G90178"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990652.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980F6"/>
    <dbReference type="FunCoup" id="Q980F6">
        <property type="interactions" value="229"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0354"/>
    <dbReference type="PaxDb" id="273057-SSO0354"/>
    <dbReference type="EnsemblBacteria" id="AAK40686">
        <property type="protein sequence ID" value="AAK40686"/>
        <property type="gene ID" value="SSO0354"/>
    </dbReference>
    <dbReference type="GeneID" id="1455496"/>
    <dbReference type="KEGG" id="sso:SSO0354"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.347"/>
    <dbReference type="eggNOG" id="arCOG01481">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043773_0_0_2"/>
    <dbReference type="InParanoid" id="Q980F6"/>
    <dbReference type="PhylomeDB" id="Q980F6"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01571">
        <property type="entry name" value="NAPRTase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.140.10">
        <property type="entry name" value="nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053190">
        <property type="entry name" value="NAPRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035809">
        <property type="entry name" value="NAPRTase_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041619">
        <property type="entry name" value="NAPRTase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006415">
        <property type="entry name" value="PRK08662.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43202:SF1">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43202">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17956">
        <property type="entry name" value="NAPRTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="9">
        <location>
            <begin position="18"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="8">
        <location>
            <begin position="115"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate phosphoribosyltransferase C-terminal" evidence="10">
        <location>
            <begin position="347"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK40686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="387" mass="43557" checksum="3F40C259DCFF896B" modified="2001-10-01" version="1">MEFYIADFKQIKEGKVTDIYFERTLKTLEYLGIKDAKVRMEVHSYGLPKGYEWALFTGLEEVLHLLEGVPVNVYAMPEGTLFKEIEPVMIIEGNYLDFGIYETAFLGILRHYSSISTKAARLKFLAMDKTLFFFGLRSLHPALAPMADRAAYIGGMDGVSGAMSKEYLGVDPSGTMPHALMLIVGDNVKAWKAFDEAMGSEVPRITLVDTFEDERTEALKAATLLGKKLYGIRLDTPSSRRGNFRKIIQEVKWTLKIHGYDHVKIFASGGIDEDDIINLRDIVDGFGIGTSIAFPPSVDFSADIVEKFVDGKWVPFTKRGKWPGAKQVYRCGKLNDVITLMDQEHPSSDCKPLLQKYMENGKIIKPLPSPKEIREYVIKQLTELQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q980K4</accession>
    <name>Q980K4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Integrase, probable</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0288</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90171">
        <property type="entry name" value="D90171"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980K4"/>
    <dbReference type="PaxDb" id="273057-SSO0288"/>
    <dbReference type="EnsemblBacteria" id="AAK40627">
        <property type="protein sequence ID" value="AAK40627"/>
        <property type="gene ID" value="SSO0288"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0288"/>
    <dbReference type="eggNOG" id="arCOG07277">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184189_0_0_2"/>
    <dbReference type="InParanoid" id="Q980K4"/>
    <dbReference type="PhylomeDB" id="Q980K4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012922">
        <property type="entry name" value="ORF_D-335"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07935">
        <property type="entry name" value="SSV1_ORF_D-335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ORF D-335-like" evidence="2">
        <location>
            <begin position="16"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="113" mass="13033" checksum="8CC4806ADED51ED9" modified="2001-10-01" version="1">MATIIQWVKIKEKQHHYKYGDIIIRERKGRYYVYKLEYENGNVKETYVDPLVDVVETYEKLKDSSGGFRGYPPNPTTDPPGFEPGTSGSGGRRSILAELRVQHKYTLYGLLNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q980L2</accession>
    <name>Q980L2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA intron endonuclease catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90170">
        <property type="entry name" value="C90170"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980L2"/>
    <dbReference type="STRING" id="273057.SSO0281"/>
    <dbReference type="PaxDb" id="273057-SSO0281"/>
    <dbReference type="EnsemblBacteria" id="AAK40618">
        <property type="protein sequence ID" value="AAK40618"/>
        <property type="gene ID" value="SSO0281"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0281"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.274"/>
    <dbReference type="eggNOG" id="arCOG01701">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129670_0_0_2"/>
    <dbReference type="InParanoid" id="Q980L2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007084">
        <property type="entry name" value="PRK09539.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA intron endonuclease catalytic" evidence="1">
        <location>
            <begin position="87"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="177" mass="20472" checksum="AE531544FD515844" modified="2001-10-01" version="1">MPIIVNIVDNCTTITTHYFSVLIIPNYFIRTTCRALHMKTITFMGNILIFDNVTIMSEKSETTLDKIYTALNSSKQLPSEIIYLVNWDKVDVFVDLKQRGRVTIDGIDEHSLIIKDKENGRYTAMVLIVDENEKVSFKKILDKLHQSKSMNLELYLSIVDKYGDVTYYTLSEIKMSK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-16" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q980L3</accession>
    <name>SYH_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histidine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Histidyl-tRNA synthetase</fullName>
            <shortName evidence="1">HisRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisS</name>
        <name type="ordered locus">SSO0279</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90170">
        <property type="entry name" value="B90170"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990560.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980L3"/>
    <dbReference type="SMR" id="Q980L3"/>
    <dbReference type="FunCoup" id="Q980L3">
        <property type="interactions" value="273"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0279"/>
    <dbReference type="PaxDb" id="273057-SSO0279"/>
    <dbReference type="EnsemblBacteria" id="AAK40617">
        <property type="protein sequence ID" value="AAK40617"/>
        <property type="gene ID" value="SSO0279"/>
    </dbReference>
    <dbReference type="GeneID" id="1455427"/>
    <dbReference type="GeneID" id="44129250"/>
    <dbReference type="KEGG" id="sso:SSO0279"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.273"/>
    <dbReference type="eggNOG" id="arCOG00404">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025113_3_1_2"/>
    <dbReference type="InParanoid" id="Q980L3"/>
    <dbReference type="PhylomeDB" id="Q980L3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000121">
        <property type="entry name" value="Histidine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00125">
        <property type="entry name" value="HisZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004517">
        <property type="entry name" value="HisZ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707:SF1">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000136326" description="Histidine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00127"/>
        </source>
    </evidence>
    <sequence length="426" mass="49144" checksum="BA68BEBEBEEE0E52" modified="2001-10-01" version="1">MVKFETVRGMKDYIGIDAEKIRYLESTFRDLAKKYGYSEIITPVVEEFKLFELKGGEELRQTMYVFKDKADREISLRPEITPSVARAYIQNLQSSPKPIRLFYFGTVYRYDEPQYGRYREFRQAGIEMIGDSSILADVEVLDLLYNFYDKLNLSKDITIKINNIGIFRKIMDKYNIEDNLQEHVLHLIDKNKVDEALVILEKNIKNKDIMDFLNMILTKKEAKLEDIESLAELEEVSKLDIKNEFEYLLRLSRILSSLNVKFKVDLGFVRGLAYYTGLIFEVLHPSVQFSIAGGGRYDKLIELYGGLPSPAIGFAIGVERTLLVIKDLKVEEPINVIVVGISEEAIPAMFTVSRMLRKEEYKVVINTKDQPLSKLLPYYASQGFKLAIIIGKQELEKNMITVRNLITRKQISIPLENVLDAIKQTL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2025-06-18" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q980U5</accession>
    <accession>Q9UWG3</accession>
    <name>GSA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate-1-semialdehyde 2,1-aminomutase</fullName>
            <shortName evidence="1">GSA</shortName>
            <ecNumber evidence="1">5.4.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamate-1-semialdehyde aminotransferase</fullName>
            <shortName evidence="1">GSA-AT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemL</name>
        <name type="ordered locus">SSO0182</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1996" name="Biochem. J." volume="320" first="541" last="545">
            <title>Glutamate-1-semialdehyde aminotransferase from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Palmieri G."/>
                <person name="Di Palo M."/>
                <person name="Scaloni A."/>
                <person name="Orru S."/>
                <person name="Marino G."/>
                <person name="Sannia G."/>
            </authorList>
            <dbReference type="PubMed" id="8973563"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-25</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Inhibited by gabaculine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40528" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="5.4.3.8" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40528.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90159">
        <property type="entry name" value="A90159"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980U5"/>
    <dbReference type="SMR" id="Q980U5"/>
    <dbReference type="FunCoup" id="Q980U5">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0182"/>
    <dbReference type="PaxDb" id="273057-SSO0182"/>
    <dbReference type="EnsemblBacteria" id="AAK40528">
        <property type="protein sequence ID" value="AAK40528"/>
        <property type="gene ID" value="SSO0182"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0182"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.180"/>
    <dbReference type="eggNOG" id="arCOG00918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_1_5_2"/>
    <dbReference type="InParanoid" id="Q980U5"/>
    <dbReference type="PhylomeDB" id="Q980U5"/>
    <dbReference type="BRENDA" id="5.4.3.8">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00317"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042286">
        <property type="term" value="F:glutamate-1-semialdehyde 2,1-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000021">
        <property type="entry name" value="Glutamate-1-semialdehyde 2,1-aminomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00375">
        <property type="entry name" value="HemL_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004639">
        <property type="entry name" value="4pyrrol_synth_GluAld_NH2Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00713">
        <property type="entry name" value="hemL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000818">
        <property type="entry name" value="PRK00062.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43713">
        <property type="entry name" value="GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43713:SF3">
        <property type="entry name" value="GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000120490" description="Glutamate-1-semialdehyde 2,1-aminomutase">
        <location>
            <begin position="1"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AA sequence." evidence="3" ref="2">
        <original>A</original>
        <variation>F</variation>
        <location>
            <position position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="8973563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="418" mass="46477" checksum="C290C1884A38E710" modified="2006-09-19" version="2">MNSEELWAQAKQLFAGGVNSPVRAAVKPFPFYVERGKGAYIYTVDGKKFIDYVLGYGPLILGHSPESVKRRIVEQLERGWLFGTPSELEIELARKIRSHIPSAQKIRFVNSGTEATMAAIRLARGYTKRSKILKFSGNYHGAHDYALVEAGSAATEYNVATSDGVPMEIMKTVEICEFNDLDCVDKKLRNEDIATVILEPVMGNAGVILPEKDFLFGLRELTKTYNSLLIFDEVITGFRISIGGAQSYYQIYPDITTLGKIIGGGFPIGAVAGKAEIIDNFTPAGKVFNAGTFNANPISMIAGIATIEELEKEYPYIIANQAAKTLVEELERLLKTKHTINHVGSMFQIFFGIDEVRNYSDAKRADKEYYIKFHERLLKEGVFIPPSQYETIFTSAAHKDDVIADTIDKLMKVIGELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q980X8</accession>
    <name>Q980X8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Arsenite transport protein (ArsB)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">arsB</name>
        <name evidence="10" type="ordered locus">SSO0144</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CitM (TC 2.A.11) transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90154">
        <property type="entry name" value="G90154"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922902.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980X8"/>
    <dbReference type="FunCoup" id="Q980X8">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0144"/>
    <dbReference type="PaxDb" id="273057-SSO0144"/>
    <dbReference type="EnsemblBacteria" id="AAK40494">
        <property type="protein sequence ID" value="AAK40494"/>
        <property type="gene ID" value="SSO0144"/>
    </dbReference>
    <dbReference type="GeneID" id="1453321"/>
    <dbReference type="KEGG" id="sso:SSO0144"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.142"/>
    <dbReference type="eggNOG" id="arCOG00238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011920_3_0_2"/>
    <dbReference type="InParanoid" id="Q980X8"/>
    <dbReference type="PhylomeDB" id="Q980X8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015105">
        <property type="term" value="F:arsenite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01117">
        <property type="entry name" value="YbiR_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000802">
        <property type="entry name" value="Arsenical_pump_ArsB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43302">
        <property type="entry name" value="TRANSPORTER ARSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43302:SF5">
        <property type="entry name" value="TRANSPORTER ARSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00758">
        <property type="entry name" value="ARSENICPUMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="311"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="347"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Citrate transporter-like" evidence="9">
        <location>
            <begin position="20"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK40494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="411" mass="45550" checksum="3139BE9EC532F00C" modified="2001-10-01" version="1">MLISALIVMVITYCLIISRSITKIPPWASMFFGGILMVILGIISPEEALQSINLDVILFLITLFTFASALEVSGFLKFLAYKIIEKFKEPRKVLFYILLYSGLLSNLVTNDGVSASWTPVILELSRMIGVSEVPFLYALAVGVTIGSVIMPTGNPQNLLIALESGIKNPFITFTIYLTLPSIISLIIAYFILFRLFRKSLSLPSGINIKKEEEEKVDFDRRLGYLTLTLLVVTIILFFSLSFFKIDILLGSLVTSSILLLLTEKRRDIVRRMDWPTILFFIGLFIFTDGVLKSGIIQYLSNFLPPPDSVASIMIVSILLSQVLSNVPLVAIYIPIMISHSGITVVDWLALAAGSTIAGNFTILGAASNVIISEASESRGGKGFNFVEFMKYTIPILIPNAIIIYLFLVLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q981B2</accession>
    <name>Q981B2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2021" name="Nucleic Acids Res." volume="49" first="13150" last="13164">
            <title>Chromosome segregation in Archaea: SegA-and SegB-DNA complex structures provide insights into segrosome assembly.</title>
            <authorList>
                <person name="Yen C.Y."/>
                <person name="Lin M.G."/>
                <person name="Chen B.W."/>
                <person name="Ng I.W."/>
                <person name="Read N."/>
                <person name="Kabli A.F."/>
                <person name="Wu C.T."/>
                <person name="Shen Y.Y."/>
                <person name="Chen C.H."/>
                <person name="Barilla D."/>
                <person name="Sun Y.J."/>
                <person name="Hsiao C.D."/>
            </authorList>
            <dbReference type="PubMed" id="34850144"/>
            <dbReference type="DOI" id="10.1093/nar/gkab1155"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) OF 34-109</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90143">
        <property type="entry name" value="A90143"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989603.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="7DUV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.20 A"/>
        <property type="chains" value="A/B/C=1-109"/>
    </dbReference>
    <dbReference type="PDB" id="7DV2">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.10 A"/>
        <property type="chains" value="A/B/C/D=34-109"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981B2"/>
    <dbReference type="SMR" id="Q981B2"/>
    <dbReference type="STRING" id="273057.SSO0035"/>
    <dbReference type="PaxDb" id="273057-SSO0035"/>
    <dbReference type="EnsemblBacteria" id="AAK40400">
        <property type="protein sequence ID" value="AAK40400"/>
        <property type="gene ID" value="SSO0035"/>
    </dbReference>
    <dbReference type="GeneID" id="1455291"/>
    <dbReference type="KEGG" id="sso:SSO0035"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.36"/>
    <dbReference type="eggNOG" id="arCOG04886">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137167_0_0_2"/>
    <dbReference type="InParanoid" id="Q981B2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4 5" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="7DUV"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="7DV2"/>
        </source>
    </evidence>
    <sequence length="109" mass="12964" checksum="63885A926FC06E96" modified="2001-10-01" version="1">MSELDFLLKKKRKSEDEEKIINNNENAKKEEITNEEEKIKNDMLKYIEKDPKIGVWSYPAFLVLQYLYHTVPGFKMSRTAKEALEKGLKEMYPTLFTIAEKIAKERFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q981E3</accession>
    <name>Q981E3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphomethylpyrimidine kinase (ThiD-1)</fullName>
            <ecNumber evidence="2">2.7.4.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">thiD-1</name>
        <name evidence="2" type="ordered locus">SSO0002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.4.7" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90139">
        <property type="entry name" value="B90139"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989649.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981E3"/>
    <dbReference type="FunCoup" id="Q981E3">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0002"/>
    <dbReference type="PaxDb" id="273057-SSO0002"/>
    <dbReference type="EnsemblBacteria" id="AAK40369">
        <property type="protein sequence ID" value="AAK40369"/>
        <property type="gene ID" value="SSO0002"/>
    </dbReference>
    <dbReference type="GeneID" id="1455259"/>
    <dbReference type="KEGG" id="sso:SSO0002"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3"/>
    <dbReference type="eggNOG" id="arCOG00020">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035788_0_0_2"/>
    <dbReference type="InParanoid" id="Q981E3"/>
    <dbReference type="PhylomeDB" id="Q981E3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858:SF17">
        <property type="entry name" value="HYDROXYMETHYLPYRIMIDINE_PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="1">
        <location>
            <begin position="14"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="400" mass="43746" checksum="166AB5C6B65E4B3A" modified="2001-10-01" version="1">MHTRPVVATIAGSDSGGGAGLQADLKTFSALGVFGTTIITGLTAQNTRTVTKVLEIPLDFIEAQFDAVCLDLHPTHAKTGMLASGKVVELVLRKIREYNIKLVLDPVMVAKSGSLLVTEDISEQIKKAMKEAIISTPNRYEAEIINKTKINSQDDVIKAAREIYSKYGNVVVKGFNGVDYAIIDGEEIELKGDYISTKNTHGSGDVFSASITAYLALGYKLKDALIRAKKFATMTVKYGLDLGGGYGPVDPFAPIESIVKREEGRNQLENLLWYLESNLNVILKLINDTSKVNVAYMTKYNDVLSLAGGLIKYLDKLKIDGPILNNVNNEITRIMRETPDEKIGILLPFTDKILDAAEKGKIKLNKSGINGDAILRDNMVLIIAKDKDELIRKLKEVAIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXE0</accession>
    <name>RL21_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Large ribosomal subunit protein eL21</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>50S ribosomal protein L21e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rpl21e</name>
        <name type="ordered locus">SSO0752</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90223">
        <property type="entry name" value="H90223"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991331.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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        <property type="gene ID" value="SSO0752"/>
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    <dbReference type="GeneID" id="1455014"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:cytosolic large ribosomal subunit"/>
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        <property type="term" value="F:structural constituent of ribosome"/>
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    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="60S ribosomal protein L21"/>
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        <property type="entry name" value="Ribosomal protein L21"/>
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        <property type="entry name" value="Ribosomal_eL21"/>
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        <property type="entry name" value="Ribosomal_eL21_arc"/>
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        <property type="entry name" value="Ribosomal_eL21_CS"/>
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        <property type="entry name" value="Ribosomal_eL21_sf"/>
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        <property type="entry name" value="60S RIBOSOMAL PROTEIN L21"/>
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        <property type="entry name" value="Ribosomal_L21e"/>
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        <property type="entry name" value="Translation proteins SH3-like domain"/>
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        <property type="entry name" value="RIBOSOMAL_L21E"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000149702" description="Large ribosomal subunit protein eL21">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="101" mass="11377" checksum="C53919378B36F760" modified="2000-05-01" version="1">MVKHSKGYRTRSRSLLRKSPRERGAVPSLSKLMVEYKEGDNVVVKINPSVHQGMPHRRYQGKVGKIIGKRGRAYLVSVTLGDKEKVIIVRPEHLVPFNSSG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-08-01" modified="2026-01-28" version="171" xmlns="https://uniprot.org/uniprot">
    <accession>P26811</accession>
    <accession>O05707</accession>
    <name>DPOL1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>DNA polymerase 1</fullName>
            <ecNumber>2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>DNA polymerase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Pol B1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">dpo1</name>
        <name type="synonym">polS</name>
        <name type="ordered locus">SSO0552</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1992" name="Nucleic Acids Res." volume="20" first="2711" last="2716">
            <title>A DNA polymerase from the archaeon Sulfolobus solfataricus shows sequence similarity to family B DNA polymerases.</title>
            <authorList>
                <person name="Pisani F.M."/>
                <person name="Martino C."/>
                <person name="Rossi M."/>
            </authorList>
            <dbReference type="PubMed" id="1614858"/>
            <dbReference type="DOI" id="10.1093/nar/20.11.2711"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2003" name="Mol. Cell" volume="11" first="275" last="282">
            <title>A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Dionne I."/>
                <person name="Nookala R.K."/>
                <person name="Jackson S.P."/>
                <person name="Doherty A.J."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="12535540"/>
            <dbReference type="DOI" id="10.1016/S1097-2765(02)00824-9"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>INTERACTION WITH PCNA3 AND PCNA2</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF 1-MET--ASP-9</scope>
    </reference>
    <comment type="function">
        <text evidence="2">This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. DNA polymerase I, DNA ligase and the flap endonuclease may be constitutively associated with the PCNA heterotrimer forming a scanning complex able to couple DNA synthesis and Okazaki fragment maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">DNA synthesis is stimulated by PCNA heterotrimers.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with PCNA subunit PCNA2 and weakly with PCNA3.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-15778666">
            <id>P26811</id>
        </interactant>
        <interactant intactId="EBI-9026921">
            <id>Q980N4</id>
            <label>cdc6-1</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>4</experiments>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-15778666">
            <id>P26811</id>
        </interactant>
        <interactant intactId="EBI-15778726">
            <id>Q9UXF8</id>
            <label>cdc6-2</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>5</experiments>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-15778666">
            <id>P26811</id>
        </interactant>
        <interactant intactId="EBI-9026919">
            <id>Q97WM8</id>
            <label>cdc6-3</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>3</experiments>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7"/>
    <dbReference type="EMBL" id="X64466">
        <property type="protein sequence ID" value="CAA45795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="U92875">
        <property type="protein sequence ID" value="AAB53090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="CAB57747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="AAK40869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A=36-882"/>
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        <property type="term" value="F:DNA binding"/>
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        <property type="term" value="F:nucleotide binding"/>
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        <property type="term" value="P:DNA-templated DNA replication"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PRK05761.1-3"/>
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        <property type="entry name" value="DNA POLYMERASE 1"/>
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        <property type="entry name" value="DNA_pol_B_exo1"/>
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</entry>
<entry dataset="Swiss-Prot" created="1995-02-01" modified="2026-01-28" version="137" xmlns="https://uniprot.org/uniprot">
    <accession>P39573</accession>
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            <fullName evidence="1 3">Small ribosomal subunit protein uS12</fullName>
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        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
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            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
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            <taxon>Sulfolobales</taxon>
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    <reference key="1">
        <citation type="journal article" date="1994" name="Biochem. Mol. Biol. Int." volume="33" first="927" last="937">
            <title>Organization of a Sulfolobus solfataricus gene cluster homologous to the Escherichia coli str operon.</title>
            <authorList>
                <person name="Ianniciello G."/>
                <person name="Gallo M."/>
                <person name="Arcari P."/>
                <person name="Bocchini V."/>
            </authorList>
            <dbReference type="PubMed" id="7527264"/>
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        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
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            <strain>DSM 5833 / MT-4</strain>
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    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
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                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
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        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
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        <text evidence="1">With S4 and S5 plays an important role in translational accuracy. Located at the interface of the 30S and 50S subunits.</text>
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        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
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        <text evidence="1 2">Part of the 30S ribosomal subunit.</text>
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</entry>
<entry dataset="Swiss-Prot" created="2001-06-20" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>P58192</accession>
    <name>RPO1C_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo1C</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit A''</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo1C</name>
        <name evidence="1" type="synonym">rpoA2</name>
        <name type="ordered locus">SSO0223</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2008" name="Nature" volume="451" first="851" last="854">
            <title>The X-ray crystal structure of RNA polymerase from Archaea.</title>
            <authorList>
                <person name="Hirata A."/>
                <person name="Klein B.J."/>
                <person name="Murakami K.S."/>
            </authorList>
            <dbReference type="PubMed" id="18235446"/>
            <dbReference type="DOI" id="10.1038/nature06530"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF THE RNA POLYMERASE COMPLEX</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms part of the jaw domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 13-subunit RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40565" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
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            <end position="336"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="338"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="350"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="365"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="368"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="377"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="380"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="384"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18235446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007744" key="4">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="5">
        <source>
            <dbReference type="PDB" id="3HKZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="3HKZ"/>
        </source>
    </evidence>
    <sequence length="392" mass="43488" checksum="5B0E871D056CE08B" modified="2001-06-20" version="1">MIDEKDKPYLEEKVKQASNILPQKIVDDLKNLILNKEIIVTRDEIDKIFDLAIKEYSEGLIAPGEAIGIVAAQSVGEPGTQMTLRTFHFAGIRELNVTLGLPRLIEIVDAKKVPSTPMMTIYLTDEYKRDRDKALEVARKLEYTKIENVVSSTSIDIASMSIILQLDNEMLKDKGVTVDDVKKAIGRLKLGDFMIEESEDSTLNINFANIDSIAALFKLRDKILNTKIKGIKGIKRAIVQKKGDEYIILTDGSNLSGVLSVKGVDVAKVETNNIREIEEVFGIEAAREIIIREISKVLAEQGLDVDIRHILLIADVMTRTGIVRQIGRHGVTGEKNSVLARAAFEVTVKHLLDAAARGDVEEFKGVVENIIIGHPIKLGTGMVELTMRPILR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-06-07" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>P61991</accession>
    <accession>O59631</accession>
    <accession>P80170</accession>
    <accession>Q9UWI8</accession>
    <name>DN7A_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-binding protein 7a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>7 kDa DNA-binding protein a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Endoribonuclease P2</fullName>
            <ecNumber>3.1.27.-</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">p7ss</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">sso7a1</name>
        <name type="synonym">sso7d-2</name>
        <name type="ordered locus">SSO9180</name>
    </gene>
    <gene>
        <name type="primary">sso7a2</name>
        <name type="synonym">sso7d-3</name>
        <name type="ordered locus">SSO9535</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1993" name="Eur. J. Biochem." volume="211" first="305" last="310">
            <title>Ribonucleases from the extreme thermophilic archaebacterium S. solfataricus.</title>
            <authorList>
                <person name="Fusi P."/>
                <person name="Tedeschi G."/>
                <person name="Aliverti A."/>
                <person name="Ronchi S."/>
                <person name="Tortora P."/>
                <person name="Guerritore A."/>
            </authorList>
            <dbReference type="PubMed" id="8425540"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1993.tb19899.x"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-63</scope>
        <scope>FUNCTION AS A RNASE</scope>
        <scope>METHYLATION AT LYS-5 AND LYS-7</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1995" name="Biochem. Biophys. Res. Commun." volume="208" first="55" last="62">
            <title>In the thermophilic archaeon Sulfolobus solfataricus a DNA-binding protein is in vitro (ADPribosyl)ated.</title>
            <authorList>
                <person name="Faraone-Mennella M.R."/>
                <person name="Farina B."/>
            </authorList>
            <dbReference type="PubMed" id="7887965"/>
            <dbReference type="DOI" id="10.1006/bbrc.1995.1304"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-40</scope>
        <scope>DNA-BINDING</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3 7">Can constrain negative DNA supercoils (By similarity). May be involved in maintaining the integrity of the genome at high temperature (Probable) (PubMed:7887965). Has RNA endonuclease activity with a narrow substrate specificity; the cleavage products are 3'-phosphooligonucleotides (PubMed:8425540).</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="3">Lys-5 and Lys-7 were found to be 60% monomethylated (PubMed:8425540).</text>
    </comment>
    <comment type="PTM">
        <text evidence="2">ADP-ribosylated by endogenous proteins in vitro (PubMed:7887965).</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the 7 kDa DNA-binding/endoribonuclease P2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.27.-"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90369">
        <property type="entry name" value="E90369"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992021.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1B4O">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=2-63"/>
    </dbReference>
    <dbReference type="PDB" id="1JIC">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=2-63"/>
    </dbReference>
    <dbReference type="PDB" id="1SSO">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=2-63"/>
    </dbReference>
    <dbReference type="PDB" id="2CVR">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=2-63"/>
    </dbReference>
    <dbReference type="PDB" id="6QBA">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="B=2-62"/>
    </dbReference>
    <dbReference type="PDBsum" id="1B4O"/>
    <dbReference type="PDBsum" id="1JIC"/>
    <dbReference type="PDBsum" id="1SSO"/>
    <dbReference type="PDBsum" id="2CVR"/>
    <dbReference type="PDBsum" id="6QBA"/>
    <dbReference type="AlphaFoldDB" id="P61991"/>
    <dbReference type="BMRB" id="P61991"/>
    <dbReference type="SMR" id="P61991"/>
    <dbReference type="STRING" id="273057.SSO9180"/>
    <dbReference type="iPTMnet" id="P61991"/>
    <dbReference type="PaxDb" id="273057-SSO9180"/>
    <dbReference type="EnsemblBacteria" id="AAK42090">
        <property type="protein sequence ID" value="AAK42090"/>
        <property type="gene ID" value="SSO9180"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK42212">
        <property type="protein sequence ID" value="AAK42212"/>
        <property type="gene ID" value="SSO9535"/>
    </dbReference>
    <dbReference type="GeneID" id="84058170"/>
    <dbReference type="KEGG" id="sso:SSO9180"/>
    <dbReference type="KEGG" id="sso:SSO9535"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1957"/>
    <dbReference type="eggNOG" id="arCOG05888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2929990_0_0_2"/>
    <dbReference type="InParanoid" id="P61991"/>
    <dbReference type="BRENDA" id="4.6.1.18">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="P61991"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003212">
        <property type="entry name" value="DNA-bd_7a-e_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045555">
        <property type="entry name" value="Sul7d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02294">
        <property type="entry name" value="7kD_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036912">
        <property type="entry name" value="Sac7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0013">ADP-ribosylation</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed" evidence="2 3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000213077" description="DNA-binding protein 7a">
        <location>
            <begin position="2"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine; partial" evidence="3">
        <location>
            <position position="5"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine; partial" evidence="3">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="4"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="11"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="17"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="22"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="29"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="37"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="44"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="turn" evidence="9">
        <location>
            <begin position="48"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="53"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P61990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="7887965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="8425540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="7887965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="8425540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <evidence type="ECO:0000305" key="7">
        <source>
            <dbReference type="PubMed" id="7887965"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="1B4O"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="6QBA"/>
        </source>
    </evidence>
    <sequence length="64" mass="7279" checksum="0E4BA126FCCF84DD" modified="2007-01-23" version="2">MATVKFKYKGEEKQVDISKIKKVWRVGKMISFTYDEGGGKTGRGAVSEKDAPKELLQMLEKQKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-02-10" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>P96026</accession>
    <accession>P95977</accession>
    <name>PELO_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein pelota homolog</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pelA</name>
        <name type="ordered locus">SSO0083</name>
        <name type="ORF">C04039</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="FEMS Microbiol. Lett." volume="144" first="151" last="155">
            <title>An archaebacterial homolog of pelota, a meiotic cell division protein in eukaryotes.</title>
            <authorList>
                <person name="Ragan M.A."/>
                <person name="Logsdon J.M. Jr."/>
                <person name="Sensen C.W."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="8900058"/>
            <dbReference type="DOI" id="10.1016/0378-1097(96)00351-5"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>GENE NAME</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May function in recognizing stalled ribosomes, interact with stem-loop structures in stalled mRNA molecules, and effect endonucleolytic cleavage of the mRNA. May play a role in the release non-functional ribosomes and degradation of damaged mRNAs. Has endoribonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The N-terminal domain has the RNA-binding Sm fold. It harbors the endoribonuclease activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="U67942">
        <property type="protein sequence ID" value="AAB49285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75405">
        <property type="entry name" value="S75405"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988896.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3OBW">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A=1-344"/>
    </dbReference>
    <dbReference type="PDBsum" id="3OBW"/>
    <dbReference type="AlphaFoldDB" id="P96026"/>
    <dbReference type="SMR" id="P96026"/>
    <dbReference type="FunCoup" id="P96026">
        <property type="interactions" value="148"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0083"/>
    <dbReference type="PaxDb" id="273057-SSO0083"/>
    <dbReference type="EnsemblBacteria" id="AAK40443">
        <property type="protein sequence ID" value="AAK40443"/>
        <property type="gene ID" value="SSO0083"/>
    </dbReference>
    <dbReference type="GeneID" id="1453271"/>
    <dbReference type="KEGG" id="sso:SSO0083"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.82"/>
    <dbReference type="eggNOG" id="arCOG01741">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023334_0_0_2"/>
    <dbReference type="InParanoid" id="P96026"/>
    <dbReference type="PhylomeDB" id="P96026"/>
    <dbReference type="EvolutionaryTrace" id="P96026"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.870:FF:000002">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000005">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01853">
        <property type="entry name" value="PelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023521">
        <property type="entry name" value="Pelota_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000361823" description="Protein pelota homolog">
        <location>
            <begin position="1"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="13"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="35"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="68"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="84"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="92"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="102"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="128"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="137"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="153"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="157"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="187"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="194"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="207"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="212"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="224"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="264"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="282"/>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="289"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="296"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="311"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="315"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="326"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="332"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="335"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01853"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="3OBW"/>
        </source>
    </evidence>
    <sequence length="344" mass="39630" checksum="C5A390FDF2271695" modified="1997-05-01" version="1">MRILEFDEKRQAVKLHIESEDDLWLLHLILEKDDKVVAKTTRDVGLGKESRRIPMTIILKVDYTEFQEFTNRLRIHGIIEDAPERFGIKGAHHTINLDIGDEIIIIKQQWNKYVLDRLKRQANKRSRIIIALVDFDEYLIAIPFEQGIKILSEKSLRPLNEEEGIIEQNALEIATELAEYVKQYDPDAILLAGPGFFKEEVSKKVNAILKNKKIYIDSVSSATRAGLHEVLKRDIIDKIMTDYEIAIGAKKMEKAMELLAKQPELVTYGLEQVKNAIEMGAVETVLVIEDLLSSDEQERLTIERMLEDIENKRGEVILVPKESPIYFELKNLTGILAILRFRIN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1995-02-01" modified="2025-06-18" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q07635</accession>
    <name>DPOL2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>DNA polymerase 2</fullName>
            <ecNumber>2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>DNA polymerase B2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>DNA polymerase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">dpo2</name>
        <name type="ordered locus">SSO1459/SSO8124</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1993" name="Nucleic Acids Res." volume="21" first="2768" last="2768">
            <title>Nucleotide sequence of the gene for a 74 kDa DNA polymerase from the archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Prangishvili D."/>
                <person name="Klenk H.-P."/>
            </authorList>
            <dbReference type="PubMed" id="8332474"/>
            <dbReference type="DOI" id="10.1093/nar/21.11.2768"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text>This polymerase is devoid of exonuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK41686" version="1"/>
        </conflict>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="frameshift">
            <sequence resource="EMBL-CDS" id="AAK41686" version="1"/>
        </conflict>
        <text>SSO1459 and SSO8124 have been merged into one gene.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="frameshift">
            <sequence resource="EMBL-CDS" id="AAK41687" version="1"/>
        </conflict>
        <text>SSO1459 and SSO8124 have been merged into one gene.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7"/>
    <dbReference type="EMBL" id="X71597">
        <property type="protein sequence ID" value="CAA50600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41686.1"/>
        <property type="status" value="ALT_FRAME"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41687.1"/>
        <property type="status" value="ALT_FRAME"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90303">
        <property type="entry name" value="G90303"/>
    </dbReference>
    <dbReference type="PIR" id="H90303">
        <property type="entry name" value="H90303"/>
    </dbReference>
    <dbReference type="PIR" id="S34120">
        <property type="entry name" value="S34120"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q07635"/>
    <dbReference type="SMR" id="Q07635"/>
    <dbReference type="STRING" id="273057.SSO1459"/>
    <dbReference type="PaxDb" id="273057-SSO1459"/>
    <dbReference type="EnsemblBacteria" id="AAK41686">
        <property type="protein sequence ID" value="AAK41686"/>
        <property type="gene ID" value="SSO1459"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41687">
        <property type="protein sequence ID" value="AAK41687"/>
        <property type="gene ID" value="SSO8124"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1459"/>
    <dbReference type="KEGG" id="sso:SSO8124"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1489"/>
    <dbReference type="eggNOG" id="arCOG00329">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_491446_0_0_2"/>
    <dbReference type="InParanoid" id="Q07635"/>
    <dbReference type="PhylomeDB" id="Q07635"/>
    <dbReference type="BRENDA" id="2.7.7.7">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05531">
        <property type="entry name" value="POLBc_B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
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        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
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        <property type="entry name" value="DNA_pol_type-B"/>
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    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000046484" description="DNA polymerase 2">
        <location>
            <begin position="1"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA50600." evidence="1" ref="1">
        <original>K</original>
        <variation>R</variation>
        <location>
            <position position="613"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA50600." evidence="1" ref="1">
        <original>E</original>
        <variation>D</variation>
        <location>
            <position position="617"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA50600." evidence="1" ref="1">
        <original>M</original>
        <variation>I</variation>
        <location>
            <position position="623"/>
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    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="626" mass="73535" checksum="21F47D2B047F03F5" modified="2001-06-20" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="154" xmlns="https://uniprot.org/uniprot">
    <accession>Q55075</accession>
    <name>RADA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>DNA repair and recombination protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">radA</name>
        <name type="ordered locus">SSO0250</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Nucleic Acids Res." volume="24" first="2125" last="2132">
            <title>recA-like genes from three archaean species with putative protein products similar to Rad51 and Dmc1 proteins of the yeast Saccharomyces cerevisiae.</title>
            <authorList>
                <person name="Sandler S.J."/>
                <person name="Satin L.H."/>
                <person name="Samra H.S."/>
                <person name="Clark A.J."/>
            </authorList>
            <dbReference type="PubMed" id="8668545"/>
            <dbReference type="DOI" id="10.1093/nar/24.11.2125"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1998" name="Genes Dev." volume="12" first="1248" last="1253">
            <title>RadA protein is an archaeal RecA protein homolog that catalyzes DNA strand exchange.</title>
            <authorList>
                <person name="Seitz E.M."/>
                <person name="Brockman J.P."/>
                <person name="Sandler S.J."/>
                <person name="Clark A.J."/>
                <person name="Kowalczykowski S.C."/>
            </authorList>
            <dbReference type="PubMed" id="9573041"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2000" name="Mol. Microbiol." volume="37" first="555" last="560">
            <title>The DNA binding and pairing preferences of the archaeal RadA protein demonstrate a universal characteristic of DNA strand exchange proteins.</title>
            <authorList>
                <person name="Seitz E.M."/>
                <person name="Kowalczykowski S.C."/>
            </authorList>
            <dbReference type="PubMed" id="10931349"/>
            <dbReference type="DOI" id="10.1046/j.1365-2958.2000.02009.x"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <comment type="function">
        <text>Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RecA-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="U45310">
        <property type="protein sequence ID" value="AAC44123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="AAK40589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="F90166"/>
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        <property type="entry name" value="S71093"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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            <end position="121"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="sequence conflict" description="In Ref. 1; AAC44123." evidence="2" ref="1">
        <original>N</original>
        <variation>I</variation>
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        </location>
    </feature>
    <feature type="helix" evidence="4">
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    </feature>
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    </feature>
    <evidence type="ECO:0000255" key="1"/>
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            <dbReference type="PDB" id="2DFL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q64214</accession>
    <name>EF1B_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Elongation factor 1-beta</fullName>
            <shortName>EF-1-beta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>aEF-1beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ef1b</name>
        <name type="ordered locus">SSO5345</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1995" name="Biochim. Biophys. Acta" volume="1263" first="86" last="88">
            <title>The first nucleotide sequence of an archaeal elongation factor 1 beta gene.</title>
            <authorList>
                <person name="Arcari P."/>
                <person name="Ianniciello G."/>
                <person name="Gallo M."/>
                <person name="Bocchini V."/>
                <person name="Raimo G."/>
            </authorList>
            <dbReference type="PubMed" id="7632739"/>
            <dbReference type="DOI" id="10.1016/0167-4781(95)00094-W"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 2-12 AND 83-91</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1996" name="Biochim. Biophys. Acta" volume="1293" first="106" last="112">
            <title>Archaeal elongation factor 1 beta is a dimer. Primary structure, molecular and biochemical properties.</title>
            <authorList>
                <person name="Raimo G."/>
                <person name="Masullo M."/>
                <person name="Savino G."/>
                <person name="Scarano G."/>
                <person name="Ianniciello G."/>
                <person name="Parente A."/>
                <person name="Bocchini V."/>
            </authorList>
            <dbReference type="PubMed" id="8652615"/>
            <dbReference type="DOI" id="10.1016/0167-4838(95)00233-2"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-91</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>MASS SPECTROMETRY</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Promotes the exchange of GDP for GTP in EF-1-alpha/GDP, thus allowing the regeneration of EF-1-alpha/GTP that could then be used to form the ternary complex EF-1-alpha/GTP/AAtRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="mass spectrometry" mass="10006.0" method="Electrospray" evidence="2"/>
    <comment type="similarity">
        <text evidence="3">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <dbReference type="EMBL" id="X76768">
        <property type="protein sequence ID" value="CAA54164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S57268">
        <property type="entry name" value="S57268"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990404.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q64214"/>
    <dbReference type="SMR" id="Q64214"/>
    <dbReference type="STRING" id="273057.SSO5345"/>
    <dbReference type="PaxDb" id="273057-SSO5345"/>
    <dbReference type="EnsemblBacteria" id="AAK40523">
        <property type="protein sequence ID" value="AAK40523"/>
        <property type="gene ID" value="SSO5345"/>
    </dbReference>
    <dbReference type="GeneID" id="1455333"/>
    <dbReference type="KEGG" id="sso:SSO5345"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.173"/>
    <dbReference type="eggNOG" id="arCOG01988">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165896_1_0_2"/>
    <dbReference type="InParanoid" id="Q64214"/>
    <dbReference type="PhylomeDB" id="Q64214"/>
    <dbReference type="BRENDA" id="3.6.5.3">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00043">
        <property type="entry name" value="EF1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004542">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00489">
        <property type="entry name" value="aEF-1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001670">
        <property type="entry name" value="PRK00435.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006521">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1 2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000155068" description="Elongation factor 1-beta">
        <location>
            <begin position="2"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="7632739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="8652615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="91" mass="10137" checksum="ED3B7CE29AF101C3" modified="2007-01-23" version="3">MTDVLVVLKVFPDSDEVNLDNLYTDISSKLPKEYKIIRKETEPIAFGLNALILYVQMPEQTEGGTDNLEEVVNNIQGVSHAEVVGITRLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX93</accession>
    <name>Q7LX93_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74014">
        <property type="entry name" value="S74014"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988667.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX93"/>
    <dbReference type="PaxDb" id="273057-SSO2102"/>
    <dbReference type="EnsemblBacteria" id="AAK42281">
        <property type="protein sequence ID" value="AAK42281"/>
        <property type="gene ID" value="SSO2102"/>
    </dbReference>
    <dbReference type="GeneID" id="1453608"/>
    <dbReference type="KEGG" id="sso:SSO2102"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2184"/>
    <dbReference type="eggNOG" id="arCOG07283">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128033_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX93"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="187" mass="21679" checksum="93AC713E04494816" modified="2011-05-31" version="1">MERKGTEIERNKISFLKWLELTLLFVILPSVVAVILSFSIPYYLLHNITLANTLSTIIPIIVFGISVAYFGKYRKSHGIITPFMKRTSIPILPDSGQPIDEKYIKSFEAGLKFVKGEEYIKRLAMIGMMYLQNAVAYDNKDLYLKAKEYLSKAEEAMKGKDVRFETRLLVDNLRSKIETYKYRFGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXE5</accession>
    <name>Q7LXE5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dTDP-4-dehydrorhamnose 3,5-epimerase</fullName>
            <ecNumber evidence="2">5.1.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Thymidine diphospho-4-keto-rhamnose 3,5-epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rfbC-1</name>
        <name evidence="3" type="ordered locus">SSO0833</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose</text>
            <dbReference type="Rhea" id="RHEA:16969"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="ChEBI" id="CHEBI:62830"/>
            <dbReference type="EC" id="5.1.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.13" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99234">
        <property type="entry name" value="A99234"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923123.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXE5"/>
    <dbReference type="FunCoup" id="Q7LXE5">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0833"/>
    <dbReference type="PaxDb" id="273057-SSO0833"/>
    <dbReference type="EnsemblBacteria" id="AAK41128">
        <property type="protein sequence ID" value="AAK41128"/>
        <property type="gene ID" value="SSO0833"/>
    </dbReference>
    <dbReference type="GeneID" id="1455095"/>
    <dbReference type="KEGG" id="sso:SSO0833"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.848"/>
    <dbReference type="eggNOG" id="arCOG04188">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090940_1_1_2"/>
    <dbReference type="InParanoid" id="Q7LXE5"/>
    <dbReference type="PhylomeDB" id="Q7LXE5"/>
    <dbReference type="UniPathway" id="UPA00124"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008830">
        <property type="term" value="F:dTDP-4-dehydrorhamnose 3,5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016854">
        <property type="term" value="F:racemase and epimerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00438">
        <property type="entry name" value="cupin_RmlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000888">
        <property type="entry name" value="RmlC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01221">
        <property type="entry name" value="rmlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21047">
        <property type="entry name" value="DTDP-6-DEOXY-D-GLUCOSE-3,5 EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21047:SF2">
        <property type="entry name" value="THYMIDINE DIPHOSPHO-4-KETO-RHAMNOSE 3,5-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00908">
        <property type="entry name" value="dTDP_sugar_isom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="site" description="Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucose" evidence="1">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU364069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="176" mass="20120" checksum="677C9A9F928A0873" modified="2011-05-31" version="1">MPFDFEDLGMGVLLIKPKVFPDKRGFFLELFKATDFSKHNIPMPVQVNMSFSVRGVVRGLHYQITPKEQGKLVFVPKGKILDVAVDVRKSSPTFGKYTSAELSESNHHMLWIPPGFAHGFQALDDSLVVYFVTHNDYSPQHERCINYSYIEWPIKEIIVSDKDKQCPPLDKAEVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXG6</accession>
    <name>Q7LXG6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0808</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90231">
        <property type="entry name" value="D90231"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923102.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXG6"/>
    <dbReference type="SMR" id="Q7LXG6"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO0808"/>
    <dbReference type="EnsemblBacteria" id="AAK41107">
        <property type="protein sequence ID" value="AAK41107"/>
        <property type="gene ID" value="SSO0808"/>
    </dbReference>
    <dbReference type="GeneID" id="1455074"/>
    <dbReference type="KEGG" id="sso:SSO0808"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.819"/>
    <dbReference type="eggNOG" id="arCOG01381">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1068007_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXG6"/>
    <dbReference type="PhylomeDB" id="Q7LXG6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="263" mass="30622" checksum="004412C9F30AA55F" modified="2011-05-31" version="1">MFSIQIPALHGKYLREVLESIREQTFQDYEVVVVNSGGGVISDIVHEYGFKEVRKDVKLLEARYLANKESRGDYAFLLDETRPLRKDALEILSKNLHDMVIIGERELGDSFWSRAAQLDKENIMECNSPEAVRGFALPRLFKRELLTASLEKLRENLREKFSQVVFPDHELIYYEASGFSNDIFVIKDELVYHYGDFTLKEIVRKYYRYGKSLKVLKDTPYSFMTSVSRKRREICVGGIKGRLTLYILYLARGIPFLFGKFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXI8</accession>
    <name>Q7LXI8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH bat-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90228">
        <property type="entry name" value="D90228"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923078.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXI8"/>
    <dbReference type="PaxDb" id="273057-SSO0785"/>
    <dbReference type="EnsemblBacteria" id="AAK41083">
        <property type="protein sequence ID" value="AAK41083"/>
        <property type="gene ID" value="SSO0785"/>
    </dbReference>
    <dbReference type="GeneID" id="1455050"/>
    <dbReference type="KEGG" id="sso:SSO0785"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.788"/>
    <dbReference type="eggNOG" id="arCOG02272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104910_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LXI8"/>
    <dbReference type="PhylomeDB" id="Q7LXI8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH bat-type" evidence="1">
        <location>
            <begin position="156"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="214" mass="24517" checksum="84D5881A97839F30" modified="2011-05-31" version="1">MGLLLVDIGMKKLYSVKVRILHKGCWTEKIKQYKIRTIKISPYGSKRVKVIIASPSHQIIKDLKESENVNEVIKYRKLNGGYLIEFSEDKDNTIAGMLLEFEDKILNYSNVINKGVEFWDFVATSKGVINSLKERFGIEDIEVKGVSLDKFLGNALTEKEVLILKIALNMGYFNYPRNIKAKDIAEILGISKQDFLYHLRNSINKIITSIDLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXJ5</accession>
    <name>Q7LXJ5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99227">
        <property type="entry name" value="A99227"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923067.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXJ5"/>
    <dbReference type="STRING" id="273057.SSO0775"/>
    <dbReference type="PaxDb" id="273057-SSO0775"/>
    <dbReference type="EnsemblBacteria" id="AAK41072">
        <property type="protein sequence ID" value="AAK41072"/>
        <property type="gene ID" value="SSO0775"/>
    </dbReference>
    <dbReference type="GeneID" id="42364227"/>
    <dbReference type="KEGG" id="sso:SSO0775"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.776"/>
    <dbReference type="eggNOG" id="arCOG07194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2079523_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXJ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="109" mass="12121" checksum="55BD2E1854245FC2" modified="2011-05-31" version="1">MPKKYNRLYNEVINSYVILILIFILIIGILGVIAFPYYISPLNNGQALNSAGYLALGVIIIIFLLLGVYFMERNEHSLGAMSILISLIILVLAIWSIYGINAVKSIFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXK7</accession>
    <name>Q7LXK7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methyltransferase small domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0748</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal PrmC-related family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90223">
        <property type="entry name" value="D90223"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991325.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXK7"/>
    <dbReference type="FunCoup" id="Q7LXK7">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0748"/>
    <dbReference type="PaxDb" id="273057-SSO0748"/>
    <dbReference type="EnsemblBacteria" id="AAK41043">
        <property type="protein sequence ID" value="AAK41043"/>
        <property type="gene ID" value="SSO0748"/>
    </dbReference>
    <dbReference type="GeneID" id="1455010"/>
    <dbReference type="KEGG" id="sso:SSO0748"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.743"/>
    <dbReference type="eggNOG" id="arCOG00109">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_6_2_2"/>
    <dbReference type="InParanoid" id="Q7LXK7"/>
    <dbReference type="PhylomeDB" id="Q7LXK7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000759">
        <property type="entry name" value="Methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052190">
        <property type="entry name" value="Euk-Arch_PrmC-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004557">
        <property type="entry name" value="PrmC-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00537">
        <property type="entry name" value="hemK_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011528">
        <property type="entry name" value="PRK14968.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875:SF1">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="5">
        <location>
            <begin position="27"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="207" mass="23483" checksum="A79D64D294CA3BD4" modified="2011-05-31" version="1">MASVRIIEFRGIRLCLNDQTYEPSDDTDILLSLLRVGKGDKVLDMGSGSGILGIWSLMMGGKVVFVDINPYATLSTLCSLKVNNLYNTPNYYGVLNCDLLSCLRKYDFDVAIFNPPYLPVEEYNEWIEYSWSGGNDGSKVLIDFLKTVKANRIYTLYSSLSDEEKIVYAINKWRFKISQKYEKVIGYERLIGLELVKEYDKGSISGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2014-06-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXL5</accession>
    <accession>Q9UXB7</accession>
    <name>XPF_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>3'-flap repair endonuclease Xpf</fullName>
            <shortName>XPF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">xpf</name>
        <name type="ordered locus">SSO0729</name>
        <name type="ORF">ORF-c10_001</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Mol. Microbiol." volume="48" first="361" last="371">
            <title>An archaeal XPF repair endonuclease dependent on a heterotrimeric PCNA.</title>
            <authorList>
                <person name="Roberts J.A."/>
                <person name="Bell S.D."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="12675797"/>
            <dbReference type="DOI" id="10.1046/j.1365-2958.2003.03444.x"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>INTERACTION WITH PCNA1 AND PCNA3</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF ASP-52 AND 228-SER--LEU-233</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">A structure-specific endonuclease, cleaves 5' of ds/ssDNA interfaces in 3' flap structures, although it also cuts bubble, Y-DNA structures and mobile and immobile Holliday junctions. Cuts preferentially after pyrimidines, may continue to progressively cleave substrate upstream of the initial cleavage, at least in vitro. May be involved in nucleotide excision repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Nuclease function requires interaction with the PCNA heterotrimer. Inhibited by SSB, even in the presence of PCNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with PCNA heterotrimer via PCNA1 and PCNA3.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90221">
        <property type="entry name" value="C90221"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991296.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXL5"/>
    <dbReference type="SMR" id="Q7LXL5"/>
    <dbReference type="STRING" id="273057.SSO0729"/>
    <dbReference type="PaxDb" id="273057-SSO0729"/>
    <dbReference type="EnsemblBacteria" id="AAK41026">
        <property type="protein sequence ID" value="AAK41026"/>
        <property type="gene ID" value="SSO0729"/>
    </dbReference>
    <dbReference type="GeneID" id="1454993"/>
    <dbReference type="GeneID" id="44129725"/>
    <dbReference type="KEGG" id="sso:SSO0729"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.726"/>
    <dbReference type="eggNOG" id="arCOG04206">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101253_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXL5"/>
    <dbReference type="PhylomeDB" id="Q7LXL5"/>
    <dbReference type="BRENDA" id="3.1.99.B2">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20075">
        <property type="entry name" value="XPF_nuclease_XPF_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053651">
        <property type="entry name" value="DNA_repair_endonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040956">
        <property type="entry name" value="Arch_Xpf_endonucase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150:SF0">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000429324" description="3'-flap repair endonuclease Xpf">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="ERCC4">
        <location>
            <begin position="8"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of nuclease activity." evidence="1">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of interaction with PCNA heterotrimer, no nuclease activity." evidence="1">
        <location>
            <begin position="228"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="12675797"/>
        </source>
    </evidence>
    <sequence length="233" mass="26683" checksum="21AD036DC5ED134E" modified="2011-05-31" version="1">MVIRIYADDREKASGIPELLKELGITVIFSQLTVADYVITDDVAVERKSVNDLVNSVFDKRFFDQISRLSEVYRFPILLVEGDINDIRKITEKWRAINNALISATIDYDVKVFYSRDKKDTAEVLKKIAEKFQFGENKSNRISLHNKAKLESVSDIQLYIVESFPNVGSILAERLLLKFGTIQNICNASISELEKALGSRKKAEDIYKILRTHYSKTNLDNDSKKTTSLFDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXU9</accession>
    <name>Q7LXU9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2286 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90201">
        <property type="entry name" value="G90201"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991060.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXU9"/>
    <dbReference type="STRING" id="273057.SSO0553"/>
    <dbReference type="PaxDb" id="273057-SSO0553"/>
    <dbReference type="EnsemblBacteria" id="AAK40870">
        <property type="protein sequence ID" value="AAK40870"/>
        <property type="gene ID" value="SSO0553"/>
    </dbReference>
    <dbReference type="GeneID" id="1454837"/>
    <dbReference type="KEGG" id="sso:SSO0553"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.563"/>
    <dbReference type="eggNOG" id="arCOG04251">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126372_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXU9"/>
    <dbReference type="PhylomeDB" id="Q7LXU9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017006">
        <property type="entry name" value="UCP032756"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10051">
        <property type="entry name" value="DUF2286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032756">
        <property type="entry name" value="UCP032756"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="17058" checksum="FDE2A8B2BE6B41E7" modified="2011-05-31" version="1">MKILIVKSENGKVTSEKVAEGEISKVLRDVAKEALEEWNELASDFIIMRDNQEVRLPLPLKPEVYEAIKTFLIGKDKKEAIAKIPVYIISYENEWKESDFQDKKIYVVSFYINDEIKKDVLNDAAQMTSEQKQEELKEELEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LYJ9</accession>
    <name>Q7LYJ9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Divalent metal cation transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2078</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74008">
        <property type="entry name" value="S74008"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LYJ9"/>
    <dbReference type="FunCoup" id="Q7LYJ9">
        <property type="interactions" value="113"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2078"/>
    <dbReference type="PaxDb" id="273057-SSO2078"/>
    <dbReference type="EnsemblBacteria" id="AAK42259">
        <property type="protein sequence ID" value="AAK42259"/>
        <property type="gene ID" value="SSO2078"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2078"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2156"/>
    <dbReference type="eggNOG" id="arCOG04531">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020088_6_0_2"/>
    <dbReference type="InParanoid" id="Q7LYJ9"/>
    <dbReference type="PhylomeDB" id="Q7LYJ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006828">
        <property type="term" value="P:manganese ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF33">
        <property type="entry name" value="NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="31702" checksum="EAC6B457BC2CD1DC" modified="2011-05-31" version="1">MSIRDSAKLFGPAWIALLADADAASILGGLSTGEEYGYKLIWFVMLLSLPIFIIQEASGRLGAIAGKGIGELIREHYSTRISILSIFPIFLVDFFTYLSEYAGIAIGCYLIGVNPLFGLIVFFILHVLVVLTKNYEITEKYLVVISVILLLSALIMVGPKLSFTGQDVFYFSTSKNFLFFIAVNVGAVVTPPCMLVYQSSATAIKYSKIGIDNSKKTSWIGIETLLGAIITELIIVLSEMIGTSIGNIDPIDPAQLLYNLGSVHYIFGITLISAGFLTLIVVSLSSAWGLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-06-18" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LYK3</accession>
    <name>Q7LYK3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73076">
        <property type="entry name" value="S73076"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LYK3"/>
    <dbReference type="FunCoup" id="Q7LYK3">
        <property type="interactions" value="209"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2072"/>
    <dbReference type="PaxDb" id="273057-SSO2072"/>
    <dbReference type="EnsemblBacteria" id="AAK42254">
        <property type="protein sequence ID" value="AAK42254"/>
        <property type="gene ID" value="SSO2072"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2072"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2149"/>
    <dbReference type="eggNOG" id="arCOG00517">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_7_3_2"/>
    <dbReference type="InParanoid" id="Q7LYK3"/>
    <dbReference type="PhylomeDB" id="Q7LYK3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050313">
        <property type="term" value="F:sulfur dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070813">
        <property type="term" value="P:hydrogen sulfide metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07724">
        <property type="entry name" value="POD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000030">
        <property type="entry name" value="Metallo-beta-lactamase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051682">
        <property type="entry name" value="Mito_Persulfide_Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044528">
        <property type="entry name" value="POD-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084">
        <property type="entry name" value="PERSULFIDE DIOXYGENASE ETHE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43084:SF1">
        <property type="entry name" value="PERSULFIDE DIOXYGENASE ETHE1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="2">
        <location>
            <begin position="13"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="247" mass="27806" checksum="7DC33E2EB1150433" modified="2011-05-31" version="1">MMIFRQIISKSGGCATYVFGCTQAGELFVVDPKYEMVDEIIELARDLGDMKIAYIIDTHTHADHLSGAKKLASLTNANVYYHELSQVKFKVERVKNGEEIKAGNVKIKVLHTPGHTPDSISVLIYDRRRDESWNEPWAILTGDTLFVGGLGRIDIGGENAEENLYYSLAKLKELPDYVEIYPAHTAGSVCGIGISGKPSSTIGFEKRFNSLFRINNKDEFVNRIREVKMPKPKEFDDYIRKNLEGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TV4</accession>
    <name>Q97TV4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transposase ISC1058</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1837</name>
        <name evidence="3" type="ordered locus">SSO1888</name>
        <name evidence="4" type="ordered locus">SSO2033</name>
        <name evidence="5" type="ordered locus">SSO3220</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90352">
        <property type="entry name" value="H90352"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923619.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TV4"/>
    <dbReference type="STRING" id="273057.SSO1837"/>
    <dbReference type="PaxDb" id="273057-SSO1837"/>
    <dbReference type="EnsemblBacteria" id="AAK42033">
        <property type="protein sequence ID" value="AAK42033"/>
        <property type="gene ID" value="SSO1837"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK42079">
        <property type="protein sequence ID" value="AAK42079"/>
        <property type="gene ID" value="SSO1888"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK42220">
        <property type="protein sequence ID" value="AAK42220"/>
        <property type="gene ID" value="SSO2033"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK43318">
        <property type="protein sequence ID" value="AAK43318"/>
        <property type="gene ID" value="SSO3220"/>
    </dbReference>
    <dbReference type="GeneID" id="1453547"/>
    <dbReference type="KEGG" id="sso:SSO1837"/>
    <dbReference type="KEGG" id="sso:SSO1888"/>
    <dbReference type="KEGG" id="sso:SSO2033"/>
    <dbReference type="KEGG" id="sso:SSO3220"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1897"/>
    <dbReference type="eggNOG" id="arCOG02754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062982_1_2_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053172">
        <property type="entry name" value="Tn903_transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053520">
        <property type="entry name" value="Transposase_Tn903"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033579">
        <property type="entry name" value="transpos_IS5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631:SF3">
        <property type="entry name" value="ISSOD12 TRANSPOSASE TNPA_ISSOD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="109"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="299" mass="34782" checksum="A49A85107429F9D3" modified="2001-10-01" version="1">MGKSKYKRDWSKYDENVITRYKLMFPFYVFEHWWDLLAEENRNARTKYKAPKEFDEFLAFLHIFLPYRAIEGVLRALEQLKIIPTSLDYSTIWKRVRNMKITFPEASDELEVIADATGISTNTGGQYIVAKWGKTRDSKFLKIEIVMDNNEFNVINAEVTSNEVESAVKTVKDLQDKGKKVKKFYGDKTYDANEVYKTGVEVVVPPKKNASTKRGHLARRKAVREFRKLGYDRWREEKGYGVRWRVESLFSAVKRTFGESVRATSFAGQVVEAKLKFWAYAWMVHLANSVVGRAPGIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TX1</accession>
    <name>Q97TX1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">First ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO11362</name>
        <name evidence="2" type="ordered locus">SSO8189</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90306">
        <property type="entry name" value="D90306"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TX1"/>
    <dbReference type="PaxDb" id="273057-SSO11362"/>
    <dbReference type="EnsemblBacteria" id="AAK41707">
        <property type="protein sequence ID" value="AAK41707"/>
        <property type="gene ID" value="SSO8189"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK42987">
        <property type="protein sequence ID" value="AAK42987"/>
        <property type="gene ID" value="SSO11362"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11362"/>
    <dbReference type="KEGG" id="sso:SSO8189"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1520"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200910_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025471">
        <property type="entry name" value="DUF4322"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14210">
        <property type="entry name" value="DUF4322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4322" evidence="1">
        <location>
            <begin position="14"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="73" mass="8148" checksum="04DE23828268E4ED" modified="2001-10-01" version="1">MRKPVLSRGEDKVITPGLPHQNNIQQVGYKLLSMLSFKGRKAEEVSRVLVSACLWNDSVESKSKGYNVRVSGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U15</accession>
    <name>Q97U15_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ornithine aminotransferase</fullName>
            <ecNumber evidence="3">2.6.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gabT-2</name>
        <name evidence="10" type="ordered locus">SSO3211</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-ornithine + 2-oxoglutarate = L-glutamate 5-semialdehyde + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:25160"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="2.6.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.13" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90506">
        <property type="entry name" value="D90506"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992747.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U15"/>
    <dbReference type="FunCoup" id="Q97U15">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3211"/>
    <dbReference type="PaxDb" id="273057-SSO3211"/>
    <dbReference type="EnsemblBacteria" id="AAK43307">
        <property type="protein sequence ID" value="AAK43307"/>
        <property type="gene ID" value="SSO3211"/>
    </dbReference>
    <dbReference type="GeneID" id="1453226"/>
    <dbReference type="KEGG" id="sso:SSO3211"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3313"/>
    <dbReference type="eggNOG" id="arCOG00915">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_0_2"/>
    <dbReference type="InParanoid" id="Q97U15"/>
    <dbReference type="PhylomeDB" id="Q97U15"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004587">
        <property type="term" value="F:ornithine aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000013">
        <property type="entry name" value="4-aminobutyrate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004426">
        <property type="entry name" value="PRK05769.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF58">
        <property type="entry name" value="LEUCINE_METHIONINE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK43307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="418" mass="46522" checksum="8D79DE9E270C1EEC" modified="2001-10-01" version="1">MSKKVDELIKEDQEYLMQSFRRWYPFVIDHGFGAIVYDIEGNEYIDFNAGIGVVALGHKNEKIINAVKEQMDKFFHYSLTDFYYEVAIEVAKRLSSLMPFSAKVFYANSGTESVEAAIKIARGHTGRQWIIGFINSFHGRTLGSLAFTSSKAIQRKSFSPLLPSTYLIPYPDRRDPLCKEECTEELLGFIEDWVFKKVVDPNEVAAFIAEPIQGEGGVIVPPKDFFYKLNNLLKKFGILLILDEVQTGIGRTGKMFAFEHFNVTPDLVCLAKALGGGLPLGAVVGRKEIMDLPPGSHANTFGGNPLALAAANVILEEVPKLLDHVSSIGKKIIEELTDTRSPYLYDVRGLGLLIGAELRKNDKPFVEGLEKVLYNSFRRGVLAIGAGESVVRIEPPLIISEELAMKGTKILKEEIEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U36</accession>
    <name>Q97U36_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2299 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90503">
        <property type="entry name" value="G90503"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991702.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U36"/>
    <dbReference type="STRING" id="273057.SSO3186"/>
    <dbReference type="PaxDb" id="273057-SSO3186"/>
    <dbReference type="EnsemblBacteria" id="AAK43286">
        <property type="protein sequence ID" value="AAK43286"/>
        <property type="gene ID" value="SSO3186"/>
    </dbReference>
    <dbReference type="GeneID" id="1453205"/>
    <dbReference type="KEGG" id="sso:SSO3186"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3291"/>
    <dbReference type="eggNOG" id="arCOG01713">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137738_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U36"/>
    <dbReference type="PhylomeDB" id="Q97U36"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd17510">
        <property type="entry name" value="T3SC_YbjN-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018747">
        <property type="entry name" value="DUF2299"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10061">
        <property type="entry name" value="DUF2299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="18353" checksum="A1B163817F65C665" modified="2001-10-01" version="1">MFDKSVDKQIEDWLRELGLVASRPPQAKEFFHVVATPPQGGPTISVVRVNDSSKFYIIAMGIGIHPTHVSALMALNREERIKFIIDLQLEALRYGVDFVALPPNQEIPNVIQVSKPIFIDGLTANEFINTLLNVRNAGVSIMLKFTQRFGPYEPQQQTSLKYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U66</accession>
    <name>Q97U66_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tryptophan repressor binding protein (WrbA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">wrbA</name>
        <name evidence="4" type="ordered locus">SSO3155</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the SsuE family. Isf subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the WrbA family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90500">
        <property type="entry name" value="A90500"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989963.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U66"/>
    <dbReference type="FunCoup" id="Q97U66">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3155"/>
    <dbReference type="PaxDb" id="273057-SSO3155"/>
    <dbReference type="EnsemblBacteria" id="AAK43256">
        <property type="protein sequence ID" value="AAK43256"/>
        <property type="gene ID" value="SSO3155"/>
    </dbReference>
    <dbReference type="GeneID" id="1453175"/>
    <dbReference type="GeneID" id="44128875"/>
    <dbReference type="KEGG" id="sso:SSO3155"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3261"/>
    <dbReference type="eggNOG" id="arCOG00510">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051402_0_2_2"/>
    <dbReference type="InParanoid" id="Q97U66"/>
    <dbReference type="PhylomeDB" id="Q97U66"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000001">
        <property type="entry name" value="NAD(P)H dehydrogenase (Quinone) FQR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF23">
        <property type="entry name" value="FLAVOPROTEIN-LIKE PROTEIN YCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="3">
        <location>
            <begin position="5"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="197" mass="21705" checksum="98D362F7670B47F3" modified="2001-10-01" version="1">MECPKILVLFYGYGSIVDLAKNVAEGAKEITKEVKLARVKEYFPQEIVNKFRIPIDTVKDIPEATLSDLEWADGIVMGSPTRYGNMTGQLKLFLDQTAELWIKGSLYGKPVGFFTEASTMHGGHESTILAMANYAYHHGMIIVPVGYGIKEVSSTMTGGSPYGASHLGNKKELDENEINIAKFLGKRVAEVAKKLRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U70</accession>
    <name>Q97U70_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF981 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90499">
        <property type="entry name" value="D90499"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991647.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U70"/>
    <dbReference type="PaxDb" id="273057-SSO3150"/>
    <dbReference type="EnsemblBacteria" id="AAK43251">
        <property type="protein sequence ID" value="AAK43251"/>
        <property type="gene ID" value="SSO3150"/>
    </dbReference>
    <dbReference type="GeneID" id="1453170"/>
    <dbReference type="KEGG" id="sso:SSO3150"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3255"/>
    <dbReference type="eggNOG" id="arCOG04349">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1363701_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U70"/>
    <dbReference type="PhylomeDB" id="Q97U70"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009324">
        <property type="entry name" value="DUF981"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06168">
        <property type="entry name" value="DUF981"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22306" checksum="7CE1A2A15CDD005F" modified="2001-10-01" version="1">MALFIDPLTSQLIAMAVSFLVLAYALFKTYRVHSTVVDYRNAMKSAYMPLLALGIFMTITGIYGLLVWPLISSYNILFYDLYPILGIGLMSIAVSVKNDYKLEVLGFMGLLYGLVTIYYGVTGYLYNMTLEPIALLALYALTGLSSIFFYPVAVFLDNVKVSKVFLIIDAVLLILAALMAGYIGLEAVPSHLAGFAKWTPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UF5</accession>
    <name>Q97UF5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Arabinose ABC transporter, arabinose binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO3066</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90488">
        <property type="entry name" value="G90488"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990876.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UF5"/>
    <dbReference type="FunCoup" id="Q97UF5">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3066"/>
    <dbReference type="TCDB" id="3.A.1.1.14">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3066"/>
    <dbReference type="EnsemblBacteria" id="AAK43166">
        <property type="protein sequence ID" value="AAK43166"/>
        <property type="gene ID" value="SSO3066"/>
    </dbReference>
    <dbReference type="GeneID" id="1453085"/>
    <dbReference type="KEGG" id="sso:SSO3066"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3166"/>
    <dbReference type="eggNOG" id="arCOG00150">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_491464_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UF5"/>
    <dbReference type="PhylomeDB" id="Q97UF5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050490">
        <property type="entry name" value="Bact_solute-bd_prot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054925">
        <property type="entry name" value="GlcS_GBP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040930">
        <property type="entry name" value="ABC_arch_GlcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43649">
        <property type="entry name" value="ARABINOSE-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43649:SF29">
        <property type="entry name" value="OSMOPROTECTIVE COMPOUNDS-BINDING PROTEIN GGTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="572"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="620" mass="69331" checksum="137FAE21403020D7" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UH1</accession>
    <name>Q97UH1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter, permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO3048</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90486">
        <property type="entry name" value="E90486"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UH1"/>
    <dbReference type="FunCoup" id="Q97UH1">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3048"/>
    <dbReference type="PaxDb" id="273057-SSO3048"/>
    <dbReference type="EnsemblBacteria" id="AAK43148">
        <property type="protein sequence ID" value="AAK43148"/>
        <property type="gene ID" value="SSO3048"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3048"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3145"/>
    <dbReference type="eggNOG" id="arCOG00751">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_0_2"/>
    <dbReference type="InParanoid" id="Q97UH1"/>
    <dbReference type="PhylomeDB" id="Q97UH1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163:SF6">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="110"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK43148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="342" mass="38384" checksum="EBCE7B7D63452BC2" modified="2001-10-01" version="1">MRRDFLIKRGIYYIIVWFVGITIDFILPRLIPGSALASIIYSRIGGAGSNYGQNVQQEIQILVQQLGLAQYQQPLYIQYFNYLKEIVTLNFGVSLTEFPVSVSTIITEAAPWTFGIVIPAIVASFFIGNLLGRVAALSRGKISDYAILGLMMFLYTFPVFVTGEILIEVLAIDLHIFPTGGQYNTFQFLKPTMSLPFIWSVIYHAILPTLTLILFSLAGWIMGMRNNMIPILQEDYMNYYRLMGVSEKMVASKAYRLALLPNFTSFSISLGYSVLGAVAIEYLFNYAGLGYFFNQAITGLDYPLLSGIFFMLVTAMVLSNFVADIIYTIIDPRTSQEETEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UK3</accession>
    <name>Q97UK3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dehydrogenase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO3008</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90481">
        <property type="entry name" value="G90481"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992660.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UK3"/>
    <dbReference type="STRING" id="273057.SSO3008"/>
    <dbReference type="PaxDb" id="273057-SSO3008"/>
    <dbReference type="DNASU" id="1453029"/>
    <dbReference type="EnsemblBacteria" id="AAK43110">
        <property type="protein sequence ID" value="AAK43110"/>
        <property type="gene ID" value="SSO3008"/>
    </dbReference>
    <dbReference type="GeneID" id="1453029"/>
    <dbReference type="KEGG" id="sso:SSO3008"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3099"/>
    <dbReference type="eggNOG" id="arCOG01622">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_1_3_2"/>
    <dbReference type="InParanoid" id="Q97UK3"/>
    <dbReference type="PhylomeDB" id="Q97UK3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000071">
        <property type="entry name" value="Dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001245">
        <property type="entry name" value="Dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840:SF3">
        <property type="entry name" value="BINDING ROSSMANN FOLD OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G10240)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="3">
        <location>
            <begin position="156"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="409" mass="46205" checksum="968B04B1AD70AAA1" modified="2001-10-01" version="1">MTLGIAFVGSGFSAKFHLRGLVGVRNVEVKGVYSRNLNSSKEFAMLSEQLGLGKPKVYSDISKMLSDKEINAVWLTVPNDVHLEYTKLIAEEVSQGRSNIHGITIEKPLARNVKEAKEMIRLVEKAGLLHGYLENQIFMPSVVRAREAIWEIGSRNSGRPYLARAAEEHSGPHNSWFWRPTISGGGALIDMTCHSLEATRFLLTDPAKTKSSLKPKTVYAETKTLKWNKKEYAQYLKEKYNVDFLKEPAEDYALTIVTYEDDLGNSVLAETRTSWNYVGPGLRLSVEVLGPEYSVNINTLQSELSVFFSRNIKLPQSEAFVEKQNADQGLMPIILDEAVVYGYQGEDRHMVESFISRKMPFENWYDGLLIVQLMMHAYLSAENGRKISFDPQKVEDFIPKVARGLYSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UL8</accession>
    <name>Q97UL8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2986</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90479">
        <property type="entry name" value="D90479"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924084.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UL8"/>
    <dbReference type="PaxDb" id="273057-SSO2986"/>
    <dbReference type="EnsemblBacteria" id="AAK43091">
        <property type="protein sequence ID" value="AAK43091"/>
        <property type="gene ID" value="SSO2986"/>
    </dbReference>
    <dbReference type="GeneID" id="1453010"/>
    <dbReference type="KEGG" id="sso:SSO2986"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3079"/>
    <dbReference type="eggNOG" id="arCOG01055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159725_2_1_2"/>
    <dbReference type="InParanoid" id="Q97UL8"/>
    <dbReference type="PhylomeDB" id="Q97UL8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038723">
        <property type="entry name" value="ArnR1-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14947">
        <property type="entry name" value="HTH_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArnR1-like winged helix-turn-helix" evidence="1">
        <location>
            <begin position="2"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="100" mass="11749" checksum="772E47108A975366" modified="2001-10-01" version="1">MRRTRDDIISDILEAIDSKINRISSIMKNVNLSASLAKKYISMLAEEGLIQDDNGEYKLTEKGRKILEQLRNMRKMELELANIIYEVRKEPVVFHQFYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UM4</accession>
    <name>Q97UM4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dinitrogenase iron-molybdenum cofactor biosynthesis domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90478">
        <property type="entry name" value="E90478"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UM4"/>
    <dbReference type="SMR" id="Q97UM4"/>
    <dbReference type="PaxDb" id="273057-SSO2980"/>
    <dbReference type="DNASU" id="1453003"/>
    <dbReference type="EnsemblBacteria" id="AAK43084">
        <property type="protein sequence ID" value="AAK43084"/>
        <property type="gene ID" value="SSO2980"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2980"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3071"/>
    <dbReference type="eggNOG" id="arCOG02739">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2044290_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UM4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="Nitrogenase accessory factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15727" checksum="818182C8EFFAD48C" modified="2001-10-01" version="1">MVRIKLFIIQVIKILMIICTVVDDSNRLDLFSRGKFIVLFTDKNKEILYKEENPALNSSTKRPLVAKECVRLRAEMVIAAHGSLCYPSYSILKKANVRMLVGNIGDSIYTYSFHSVSKWEVTYSSFLAIKERLFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UM6</accession>
    <name>Q97UM6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="2">M1Pi</shortName>
            <shortName evidence="2">MTR-1-P isomerase</shortName>
            <ecNumber evidence="2">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">MTNA-like protein</fullName>
            <shortName evidence="2">aMTNA</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">eiF2B</name>
        <name evidence="3" type="ordered locus">SSO2978</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-(methylsulfanyl)-alpha-D-ribose 1-phosphate = 5-(methylsulfanyl)-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90478">
        <property type="entry name" value="C90478"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992577.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UM6"/>
    <dbReference type="FunCoup" id="Q97UM6">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2978"/>
    <dbReference type="PaxDb" id="273057-SSO2978"/>
    <dbReference type="EnsemblBacteria" id="AAK43082">
        <property type="protein sequence ID" value="AAK43082"/>
        <property type="gene ID" value="SSO2978"/>
    </dbReference>
    <dbReference type="GeneID" id="1453001"/>
    <dbReference type="KEGG" id="sso:SSO2978"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3069"/>
    <dbReference type="eggNOG" id="arCOG01123">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016218_1_2_2"/>
    <dbReference type="InParanoid" id="Q97UM6"/>
    <dbReference type="PhylomeDB" id="Q97UM6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.420:FF:000003">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000006">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="entry name" value="translation initiation factor eif-2b, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01678">
        <property type="entry name" value="Salvage_MtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004326">
        <property type="entry name" value="PRK05720.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004436">
        <property type="entry name" value="PRK05772.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475:SF1">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1 2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="61"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="268"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="2">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="366" mass="40339" checksum="5C5A6DD78B8DE41A" modified="2001-10-01" version="1">MKLTVKEVKEVYKPKLLPIIWKDENNTLTILDQSLLPFQKVYVDLKDVDSTALAIKNMQVRGAPAIGITAGYGMVLALTSNKNVKTLDEAIRELTRAKAILDSARPTAVNLVWATSRMLTLAKNAVENGNAKTINELIDLMKVEAKRIFDEEYDAEIQMGLYGLEKLNDSDTVLTQCNAGGLATGTGLGTALSPVKLAKALGMNVSVIAPETRPWLQGSRLTVYELMEEGIKVTLITDTAVGLVMYRGMVNSVMVGADRILRDGHVFNKIGTYKEAVIAHELGIPFYALAPTSSFDLKSNVNEIKIEERDPNEVRTIRGIPIAPENVNVYNPVFDVTPPKYIAGIITEKGIIYPPFHENIKRIVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UT0</accession>
    <name>Q97UT0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2914</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90470">
        <property type="entry name" value="G90470"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UT0"/>
    <dbReference type="STRING" id="273057.SSO2914"/>
    <dbReference type="PaxDb" id="273057-SSO2914"/>
    <dbReference type="EnsemblBacteria" id="AAK43022">
        <property type="protein sequence ID" value="AAK43022"/>
        <property type="gene ID" value="SSO2914"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2914"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3003"/>
    <dbReference type="eggNOG" id="arCOG05628">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054750_5_2_2"/>
    <dbReference type="InParanoid" id="Q97UT0"/>
    <dbReference type="PhylomeDB" id="Q97UT0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052017">
        <property type="entry name" value="TSUP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30269:SF38">
        <property type="entry name" value="SULFITE EXPORTER TAUE_SAFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30269">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN YFCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK43022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="284" mass="31759" checksum="331D46FE534428F7" modified="2001-10-01" version="1">MMLDLIYLSVIIFIASIPTGAFAYGFSSTATPLLLIRYTNKQISPILNFIEMFQNLTNVILNFKNYSIRSLVFTIFGTPGVLIGSIVGAYILKISANETLFKVIVYSILIPLILLQAAGFRKEIGLEKWKKIGYLVTFPVGVLYGVTTISGPPLALIINNQGLTKQEFKFAIAVLRTVESVSTFGAYLTLGIFNPQVIYYATVTSPAVIAGMLIGIFMGNVIKHKEDFRRLVMSFDSWISGYGLSFNLAKVVDYGVWYWPMTAIIAIDLYLLLRYIKGRRRLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UV3</accession>
    <name>Q97UV3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Third ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2889</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90467">
        <property type="entry name" value="F90467"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UV3"/>
    <dbReference type="STRING" id="273057.SSO2889"/>
    <dbReference type="PaxDb" id="273057-SSO2889"/>
    <dbReference type="EnsemblBacteria" id="AAK42997">
        <property type="protein sequence ID" value="AAK42997"/>
        <property type="gene ID" value="SSO2889"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2889"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2977"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074024_1_0_2"/>
    <dbReference type="InParanoid" id="Q97UV3"/>
    <dbReference type="PhylomeDB" id="Q97UV3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="32"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="246" mass="28898" checksum="32FBF4ADCAACAFB3" modified="2001-10-01" version="1">MVSRWRDWVVQPRGTRGTTTATTKYQNMVLLLAFVPQVNGMSKDEIVKLLMEQIVGMGLKVGLVTLDAGFYTVEVLKFISQFKFVIGVPVGDVKIYEEFDGEYTTNSKRHKKEEQVKFRLLVYGKEIVKKRKKTVVYFARATNLDLPKREVLKLYNKVRSPIETSYRNIKAFLPFTSSTKFIFRELIFVLAMIFYSLYTVFKNVMTREEFRLLLILCFLDDLSDLKDFIFNLEETLINTIDLFLWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V21</accession>
    <name>Q97V21_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="3">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO2815</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90458">
        <property type="entry name" value="G90458"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989033.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V21"/>
    <dbReference type="SMR" id="Q97V21"/>
    <dbReference type="FunCoup" id="Q97V21">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2815"/>
    <dbReference type="PaxDb" id="273057-SSO2815"/>
    <dbReference type="EnsemblBacteria" id="AAK42926">
        <property type="protein sequence ID" value="AAK42926"/>
        <property type="gene ID" value="SSO2815"/>
    </dbReference>
    <dbReference type="GeneID" id="1452845"/>
    <dbReference type="KEGG" id="sso:SSO2815"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2901"/>
    <dbReference type="eggNOG" id="arCOG01606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017038_1_0_2"/>
    <dbReference type="InParanoid" id="Q97V21"/>
    <dbReference type="PhylomeDB" id="Q97V21"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000022">
        <property type="entry name" value="2-oxoglutarate ferredoxin oxidoreductase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000009">
        <property type="entry name" value="2-oxoglutarate ferredoxin oxidoreductase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022367">
        <property type="entry name" value="2-oxoacid/accept_OxRdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053400">
        <property type="entry name" value="2-oxoacid_Fdx_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03710">
        <property type="entry name" value="OAFO_sf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041170">
        <property type="entry name" value="Oxoac_fdxalpha_Archa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF16">
        <property type="entry name" value="PYRUVATE FLAVODOXIN_FERREDOXIN OXIDOREDUCTASE DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="7">
        <location>
            <begin position="12"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="8">
        <location>
            <begin position="242"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate:ferredoxin oxidoreductase core" evidence="9">
        <location>
            <begin position="518"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="632" mass="70228" checksum="6648EF7531835277" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V77</accession>
    <name>Q97V77_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pyruvate synthase delta chain (Pyruvic-ferredoxin oxidoreductase delta chain) (PorD-2)</fullName>
            <ecNumber evidence="9">1.2.7.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">porD-2</name>
        <name evidence="9" type="ordered locus">SSO11071</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.2.7.1" evidence="9"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90451">
        <property type="entry name" value="E90451"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V77"/>
    <dbReference type="FunCoup" id="Q97V77">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO11071"/>
    <dbReference type="PaxDb" id="273057-SSO11071"/>
    <dbReference type="EnsemblBacteria" id="AAK42868">
        <property type="protein sequence ID" value="AAK42868"/>
        <property type="gene ID" value="SSO11071"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11071"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2837"/>
    <dbReference type="eggNOG" id="arCOG01605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_1_1_2"/>
    <dbReference type="InParanoid" id="Q97V77"/>
    <dbReference type="PhylomeDB" id="Q97V77"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011898">
        <property type="entry name" value="PorD_KorD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02179">
        <property type="entry name" value="PorD_KorD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724:SF1">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="35"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="64"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="94" mass="10312" checksum="A9592C3A4307C5F3" modified="2001-10-01" version="1">MDKLVSSMSLLEYQYFPITRPNKGAGGRTGAWRIVKPVVDYSKCIGCKACFMFCPESTIVPSNGKVRVDYEYCKGCGVCANVCPVKAISMVSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V81</accession>
    <name>Q97V81_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2754</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90451">
        <property type="entry name" value="A90451"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990257.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V81"/>
    <dbReference type="FunCoup" id="Q97V81">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2754"/>
    <dbReference type="CAZy" id="GH15">
        <property type="family name" value="Glycoside Hydrolase Family 15"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2754"/>
    <dbReference type="EnsemblBacteria" id="AAK42864">
        <property type="protein sequence ID" value="AAK42864"/>
        <property type="gene ID" value="SSO2754"/>
    </dbReference>
    <dbReference type="GeneID" id="1452783"/>
    <dbReference type="KEGG" id="sso:SSO2754"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2833"/>
    <dbReference type="eggNOG" id="arCOG03286">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010399_3_1_2"/>
    <dbReference type="InParanoid" id="Q97V81"/>
    <dbReference type="PhylomeDB" id="Q97V81"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053494">
        <property type="entry name" value="GH15_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045582">
        <property type="entry name" value="Trehalase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054963">
        <property type="entry name" value="Trehalase_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041084">
        <property type="entry name" value="trehalase_H1_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041085">
        <property type="entry name" value="trehalase_H2_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616:SF0">
        <property type="entry name" value="GLUCAN 1,4-ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19291">
        <property type="entry name" value="TREH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trehalase-like N-terminal" evidence="3">
        <location>
            <begin position="7"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="GH15-like" evidence="2">
        <location>
            <begin position="211"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="584" mass="67204" checksum="BBDA8F05002A777D" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-05-29" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V99</accession>
    <name>Q97V99_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Carbonic anhydrase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2735</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90448">
        <property type="entry name" value="G90448"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V99"/>
    <dbReference type="STRING" id="273057.SSO2735"/>
    <dbReference type="PaxDb" id="273057-SSO2735"/>
    <dbReference type="EnsemblBacteria" id="AAK42846">
        <property type="protein sequence ID" value="AAK42846"/>
        <property type="gene ID" value="SSO2735"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2735"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2815"/>
    <dbReference type="eggNOG" id="arCOG02860">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118421_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V99"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03379">
        <property type="entry name" value="beta_CA_cladeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="beta-carbonic anhydrase, cab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601765-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="25748" checksum="AEEC637164C508A1" modified="2001-10-01" version="1">MFNRYFLNKRLNLLMSIFTVMRLVVSCMDRRLNSYIKKKYQDAIVLRNAGANVNSLLMSLEKFNNKVDEVILLPHTDCGAMKVVYSSLKEGKKITSLVEEKLVSQFSSKKFSSLSELERLNMEIQEENLKRIFGDKVRAELVDVNKIEIPPSNDPYMVYVTVPSQLVRLSSNIYHISAEDKEIWDSLDIAVYAMNITKIISQNDKLAEKIRNMYPSVTVSTASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VP7</accession>
    <name>Q97VP7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2569</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90430">
        <property type="entry name" value="A90430"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991794.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VP7"/>
    <dbReference type="PaxDb" id="273057-SSO2569"/>
    <dbReference type="EnsemblBacteria" id="AAK42696">
        <property type="protein sequence ID" value="AAK42696"/>
        <property type="gene ID" value="SSO2569"/>
    </dbReference>
    <dbReference type="GeneID" id="1454023"/>
    <dbReference type="KEGG" id="sso:SSO2569"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2651"/>
    <dbReference type="eggNOG" id="arCOG05922">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_867721_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VP7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="330" mass="38401" checksum="71D097F0784EC67B" modified="2001-10-01" version="1">MPSLFGKKVKVIHHIDQLHSTMKLAIKTILDSYLPDVVRGYGFKYADPIWGEPIFIPYGYLDGEFKDTIDAFKKIMEEINERKEDGLAKFKEWYPDAKFFDIYRFIQYSIPGTEEGYTPGIAVDPLMPYNYFKDGLNEVKDEVKGEVVVASPSLSSFTEFKFYDPIIGRRNEIVDAYIWINKLFHEQYDKDKMYDEKLGRHYMNTILDFLEGYSKKGRVNEIEGGDVLLIPMFIWGKDKLFNDNSNIVSAWQNSKLLTSSMFHEIEALPVILNKQYFDSIVNRCSQTFTKIILLSNKKLPQIDKCNECPSSLRLLKLQKEGNFSKVFITK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VS5</accession>
    <name>PCKG_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoenolpyruvate carboxykinase [GTP]</fullName>
            <shortName evidence="1">PEP carboxykinase</shortName>
            <shortName evidence="1">PEPCK</shortName>
            <ecNumber evidence="1">4.1.1.32</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pckG</name>
        <name type="ordered locus">SSO2537</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>oxaloacetate + GTP = phosphoenolpyruvate + GDP + CO2</text>
            <dbReference type="Rhea" id="RHEA:10388"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.1.1.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 1 Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.32" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90426">
        <property type="entry name" value="C90426"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990986.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VS5"/>
    <dbReference type="SMR" id="Q97VS5"/>
    <dbReference type="FunCoup" id="Q97VS5">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2537"/>
    <dbReference type="PaxDb" id="273057-SSO2537"/>
    <dbReference type="EnsemblBacteria" id="AAK42666">
        <property type="protein sequence ID" value="AAK42666"/>
        <property type="gene ID" value="SSO2537"/>
    </dbReference>
    <dbReference type="GeneID" id="1453993"/>
    <dbReference type="KEGG" id="sso:SSO2537"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2613"/>
    <dbReference type="eggNOG" id="arCOG05865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028872_1_1_2"/>
    <dbReference type="InParanoid" id="Q97VS5"/>
    <dbReference type="PhylomeDB" id="Q97VS5"/>
    <dbReference type="BRENDA" id="4.1.1.32">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004613">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (GTP) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071333">
        <property type="term" value="P:cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046327">
        <property type="term" value="P:glycerol biosynthetic process from pyruvate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019543">
        <property type="term" value="P:propionate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033993">
        <property type="term" value="P:response to lipid"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00819">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.8.10:FF:000011">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase [GTP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.449.10:FF:000010">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase [GTP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.449.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.8.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00452">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013035">
        <property type="entry name" value="PEP_carboxykinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008209">
        <property type="entry name" value="PEP_carboxykinase_GTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035077">
        <property type="entry name" value="PEP_carboxykinase_GTP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035078">
        <property type="entry name" value="PEP_carboxykinase_GTP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003253">
        <property type="entry name" value="PRK04210.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11561">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11561:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE [GTP]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00821">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17297">
        <property type="entry name" value="PEPCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001348">
        <property type="entry name" value="PEP_carboxykinase_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="PEP carboxykinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53795">
        <property type="entry name" value="PEP carboxykinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0312">Gluconeogenesis</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000103622" description="Phosphoenolpyruvate carboxykinase [GTP]">
        <location>
            <begin position="1"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="209"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="259"/>
            <end position="264"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="365"/>
            <end position="367"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00452"/>
        </source>
    </evidence>
    <sequence length="603" mass="69057" checksum="DA4CE81C0BA84F13" modified="2001-09-26" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W32</accession>
    <name>Q97W32_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90412">
        <property type="entry name" value="G90412"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989420.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W32"/>
    <dbReference type="STRING" id="273057.SSO2410"/>
    <dbReference type="PaxDb" id="273057-SSO2410"/>
    <dbReference type="EnsemblBacteria" id="AAK42558">
        <property type="protein sequence ID" value="AAK42558"/>
        <property type="gene ID" value="SSO2410"/>
    </dbReference>
    <dbReference type="GeneID" id="1453885"/>
    <dbReference type="KEGG" id="sso:SSO2410"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2493"/>
    <dbReference type="eggNOG" id="arCOG05951">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151091_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W32"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="125" mass="14670" checksum="E8B4F6DDD49FA29E" modified="2001-10-01" version="1">MKLLLRVIEDLSSLFIEWYGDYVKKIIVGGKSFEKNDETEETIFLITLDKVSKISLYARGEIFSFFYNKVRNKETTKDFILNYGILPKIYGLLLSPKELEYEIPLLEYLNTHGRVIYSVEEEKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W58</accession>
    <name>Q97W58_SACS2</name>
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        <recommendedName>
            <fullName evidence="3">DUF424 family protein</fullName>
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    <gene>
        <name evidence="1" type="ordered locus">SSO2382</name>
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    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AAK42530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="C90409"/>
    </dbReference>
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    <dbReference type="STRING" id="273057.SSO2382"/>
    <dbReference type="PaxDb" id="273057-SSO2382"/>
    <dbReference type="EnsemblBacteria" id="AAK42530">
        <property type="protein sequence ID" value="AAK42530"/>
        <property type="gene ID" value="SSO2382"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2382"/>
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    <dbReference type="HOGENOM" id="CLU_174522_0_0_2"/>
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    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1860.10">
        <property type="entry name" value="uncharacterized conserved protein from methanopyrus kandleri domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF424"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04242">
        <property type="entry name" value="DUF424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12389" checksum="2A03845025B846D4" modified="2001-10-01" version="1">MSSMWRSNTSETTMKVLLNIIRAQGMTLINICQEELLGKVFREGEIILDISEKFYGGESVELDYALSLIDEATVMSIVGNYAVEEAIRRGLVHKDSVINVAGIKFAQVYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="139" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W95</accession>
    <name>Q97W95_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Purine phosphoribosyltransferase (GpT-1)</fullName>
            <ecNumber evidence="4">2.4.2.-</ecNumber>
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    </protein>
    <gene>
        <name evidence="4" type="primary">gpT-1</name>
        <name evidence="4" type="ordered locus">SSO2342</name>
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    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="2">
        <citation type="journal article" date="2015" name="Biochemistry" volume="54" first="2323" last="2334">
            <title>Adenine phosphoribosyltransferase from Sulfolobus solfataricus is an enzyme with unusual kinetic properties and a crystal structure that suggests it evolved from a 6-oxopurine phosphoribosyltransferase.</title>
            <authorList>
                <person name="Jensen K.F."/>
                <person name="Hansen M.R."/>
                <person name="Jensen K.S."/>
                <person name="Christoffersen S."/>
                <person name="Poulsen J.C."/>
                <person name="Molgaard A."/>
                <person name="Kadziola A."/>
            </authorList>
            <dbReference type="PubMed" id="25790177"/>
            <dbReference type="DOI" id="10.1021/bi501334m"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) IN COMPLEX WITH ADP; AMP AND ADENINE</scope>
    </reference>
    <dbReference type="EC" id="2.4.2.-" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90404">
        <property type="entry name" value="F90404"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989517.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="4TRB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=1-210"/>
    </dbReference>
    <dbReference type="PDB" id="4TRC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=1-210"/>
    </dbReference>
    <dbReference type="PDB" id="4TS5">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=1-210"/>
    </dbReference>
    <dbReference type="PDB" id="4TS7">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B=1-210"/>
    </dbReference>
    <dbReference type="PDBsum" id="4TRB"/>
    <dbReference type="PDBsum" id="4TRC"/>
    <dbReference type="PDBsum" id="4TS5"/>
    <dbReference type="PDBsum" id="4TS7"/>
    <dbReference type="AlphaFoldDB" id="Q97W95"/>
    <dbReference type="SMR" id="Q97W95"/>
    <dbReference type="FunCoup" id="Q97W95">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2342"/>
    <dbReference type="PaxDb" id="273057-SSO2342"/>
    <dbReference type="EnsemblBacteria" id="AAK42493">
        <property type="protein sequence ID" value="AAK42493"/>
        <property type="gene ID" value="SSO2342"/>
    </dbReference>
    <dbReference type="GeneID" id="1453820"/>
    <dbReference type="KEGG" id="sso:SSO2342"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2426"/>
    <dbReference type="eggNOG" id="arCOG00040">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080904_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W95"/>
    <dbReference type="PhylomeDB" id="Q97W95"/>
    <dbReference type="BRENDA" id="2.4.2.7">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97W95"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="6 7 8" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="3">
        <location>
            <begin position="11"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>adenine</name>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
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        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
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        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
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        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
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        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>adenine</name>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
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        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
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        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>adenine</name>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="4TRB"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="4TRC"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="4TS5"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="4TS7"/>
        </source>
    </evidence>
    <sequence length="210" mass="24297" checksum="EADCBE8B1EE591EB" modified="2001-10-01" version="1">MQKIPVKVVTWDEIVSLSTKLAEKIKADEYNVNVIVAIARGGLVPARLVADVLGVFDILSIKIEHWIETASHTPEAKVKYPFKVDLSDKNVLIIDDITDTGDSIELARKYVMENFRPTEVKTATLQYIKPAAKIIPDYYAEEIVSWAWFMYPWNYWEDEINLVNKILIERKTKDIDINELKRNFVESYGIENPPISLDKILTEMKRRKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WB4</accession>
    <name>Q97WB4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transposase ISC1173</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2320</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Absolutely required for transposition of IS1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase 27 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90402">
        <property type="entry name" value="C90402"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WB4"/>
    <dbReference type="PaxDb" id="273057-SSO2320"/>
    <dbReference type="EnsemblBacteria" id="AAK42474">
        <property type="protein sequence ID" value="AAK42474"/>
        <property type="gene ID" value="SSO2320"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2320"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2408"/>
    <dbReference type="eggNOG" id="arCOG02133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076276_0_1_2"/>
    <dbReference type="InParanoid" id="Q97WB4"/>
    <dbReference type="PhylomeDB" id="Q97WB4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005063">
        <property type="entry name" value="Transposase_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051354">
        <property type="entry name" value="Transposase_27_IS1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033558">
        <property type="entry name" value="transpos_IS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293:SF1">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03400">
        <property type="entry name" value="DDE_Tnp_IS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37683" checksum="DE700B417F93D878" modified="2001-10-01" version="1">MRVTRMNLVTLAQLILMILRNLNLKPRKYELKDIALALAAYSLGVQITKTGIPPSTLYYYLRKVGIRRRRESRPTCPSCNSNRVVKNGSSRGKAKYKCKACGKTFYDVTSHRMDREQRERILKEYLNRMSMRAISRVEGKPLTTVYSLVRRIGIRAFTNLTILKGELKNFTAKSTVFDEFWTYFRVRHGVMREDLWIWTALADGVPFYEFGDRSYGTFRYLLGWLPRSEVNYTDYYCVYQVLDNRVAGKKYTYLVESHNSWCRSHLARLARDTKAVTRSGRMTEYSLALLNVMYPHVFSRERTPLNEAYLKGVQYIRENLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WC8</accession>
    <name>Q97WC8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cobalamin biosynthesis precorrin-2 methyltransferase, putative (CbiL)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cbiL</name>
        <name evidence="9" type="ordered locus">SSO2301</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90400">
        <property type="entry name" value="E90400"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991809.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WC8"/>
    <dbReference type="FunCoup" id="Q97WC8">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2301"/>
    <dbReference type="PaxDb" id="273057-SSO2301"/>
    <dbReference type="EnsemblBacteria" id="AAK42460">
        <property type="protein sequence ID" value="AAK42460"/>
        <property type="gene ID" value="SSO2301"/>
    </dbReference>
    <dbReference type="GeneID" id="1453787"/>
    <dbReference type="KEGG" id="sso:SSO2301"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2392"/>
    <dbReference type="eggNOG" id="arCOG00648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076014_2_0_2"/>
    <dbReference type="InParanoid" id="Q97WC8"/>
    <dbReference type="PhylomeDB" id="Q97WC8"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030788">
        <property type="term" value="F:precorrin-2 C20-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11645">
        <property type="entry name" value="Precorrin_2_C20_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012382">
        <property type="entry name" value="CobI/CbiL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006364">
        <property type="entry name" value="CobI/CbiL/CobIJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01467">
        <property type="entry name" value="cobI_cbiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004062">
        <property type="entry name" value="PRK05576.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43467">
        <property type="entry name" value="COBALT-PRECORRIN-2 C(20)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43467:SF2">
        <property type="entry name" value="COBALT-PRECORRIN-2 C(20)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036427">
        <property type="entry name" value="Precrrn-2_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="8">
        <location>
            <begin position="2"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="222" mass="24702" checksum="0C59CCBDA1D378FE" modified="2001-10-01" version="1">MKLYVVGLGPGDDELITLRGAKILKEVRTIFIPYSTGTNRSLAENIVKKYADNNSKLVLLGFPMAKDVSENELKKLGEKICSESEGDSAFVTLGDPTLYSTFFRIKNKLPCSIDVEIVPGVSSITACASKAMISLANSEDSISIIPASRLDTIAKASEVFETIIVLKANENIKEIADILSKKYDLIYARRCFMSDEKLVNMEREIINDKDYFSMIIALRKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WH7</accession>
    <name>Q97WH7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC774</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO10075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90394">
        <property type="entry name" value="C90394"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WH7"/>
    <dbReference type="STRING" id="273057.SSO10075"/>
    <dbReference type="PaxDb" id="273057-SSO10075"/>
    <dbReference type="EnsemblBacteria" id="AAK42410">
        <property type="protein sequence ID" value="AAK42410"/>
        <property type="gene ID" value="SSO10075"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO10075"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2337"/>
    <dbReference type="eggNOG" id="arCOG02134">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2230495_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WH7"/>
    <dbReference type="PhylomeDB" id="Q97WH7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="84" mass="9751" checksum="71B919CC0C31F87D" modified="2001-10-01" version="1">MHDGLSVYNAFDWFNVGHKRVTLGDYTEEGSRKLKHRVSSMDFACNSNKLMSWFSTFFLIYNLLYIQVYLVDKGVMINANISTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WJ4</accession>
    <name>Q97WJ4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SAM-dependent methyltransferase TRM5/TYW2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2223</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90392">
        <property type="entry name" value="A90392"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WJ4"/>
    <dbReference type="SMR" id="Q97WJ4"/>
    <dbReference type="STRING" id="273057.SSO2223"/>
    <dbReference type="PaxDb" id="273057-SSO2223"/>
    <dbReference type="EnsemblBacteria" id="AAK42392">
        <property type="protein sequence ID" value="AAK42392"/>
        <property type="gene ID" value="SSO2223"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2223"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2319"/>
    <dbReference type="eggNOG" id="arCOG00033">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022610_0_1_2"/>
    <dbReference type="InParanoid" id="Q97WJ4"/>
    <dbReference type="PhylomeDB" id="Q97WJ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000698">
        <property type="entry name" value="Putative methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="6">
        <location>
            <begin position="46"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="276" mass="31606" checksum="08F4D555B5DE6620" modified="2001-10-01" version="1">MWSLMHPYLPATVRKWTNLNLSNHSFSLNTMKTKRLSEFFPGVRSYYIVGEIAIITPKRVNVDYNLVAEKIMQAHPKIKAVYLKKKVKGELRTNELEFLSGERISSTIYKENGVLFYVDINKVYVNPSLSGDRLKNLELVEEGSTVLDAFTGYGAIALNIAHKKRVYVVAGDINIDGLYMLKKSLSLNKIKGMIDIVQYDAHHLPFRDKVFKLSFGDNPTLIIDFKEELCRVSENVVFYILCESEDKANSSLGRTSWIKINDYSKNLFIFKGLVRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WY1</accession>
    <name>Q97WY1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposase ISC1332</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1980</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90364">
        <property type="entry name" value="C90364"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923708.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WY1"/>
    <dbReference type="STRING" id="273057.SSO1980"/>
    <dbReference type="PaxDb" id="273057-SSO1980"/>
    <dbReference type="EnsemblBacteria" id="AAK42170">
        <property type="protein sequence ID" value="AAK42170"/>
        <property type="gene ID" value="SSO1980"/>
    </dbReference>
    <dbReference type="GeneID" id="1453497"/>
    <dbReference type="KEGG" id="sso:SSO1980"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2056"/>
    <dbReference type="eggNOG" id="arCOG04918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036805_2_0_2"/>
    <dbReference type="InParanoid" id="Q97WY1"/>
    <dbReference type="PhylomeDB" id="Q97WY1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033543">
        <property type="entry name" value="transpos_IS256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217:SF7">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00872">
        <property type="entry name" value="Transposase_mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="414" mass="48132" checksum="D497B00E2DCB7D60" modified="2001-10-01" version="1">MTKSKNNPGRGNTIRESNTMKVMEEIREKIRKAIKEGVSLREIEEIILQEAMMEEREAYLEVEDDHKNGTYFRYLGTGDGVLRLRVPRTRKGGFRPKILPEKYERTSPDYEDFLQQLVLSGMTPSQVKAVLAAKGIPYSEIVMNRVAERISNKLKEYKSRELPHDLLALYIDVKIVKVRISESIMERAIYIAIGVDLEGNKFVLDYEVRDREDLDGWKSFLSGLVSRGVSRVDVIVSDDFSGLDRVVSTLFPSSQHQLCITHMVRNLMRVLPDKEKEELMIRVRDLKSSRNVEEGRKAILSLSQLVQPFSPARAKRLLDAADKYCSFLNFPREIRHYLYTNNTSESFNLTLARFEEELGGYFPSLRSLEVYLYVSIHESNSRWKFRPMSVIRHYSYHLKQLHASRFQVSLDEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WZ3</accession>
    <name>Q97WZ3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Third ORF in transposon ISC1913</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1967</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90362">
        <property type="entry name" value="G90362"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WZ3"/>
    <dbReference type="STRING" id="273057.SSO1967"/>
    <dbReference type="PaxDb" id="273057-SSO1967"/>
    <dbReference type="EnsemblBacteria" id="AAK42158">
        <property type="protein sequence ID" value="AAK42158"/>
        <property type="gene ID" value="SSO1967"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1967"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2041"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_17_0_2"/>
    <dbReference type="InParanoid" id="Q97WZ3"/>
    <dbReference type="PhylomeDB" id="Q97WZ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="295"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="410" mass="47821" checksum="E81AB0A9FE59F81D" modified="2001-10-01" version="1">MERTVKLRVKVDYSTYSALKEVEKEYREVLEDAINYGLSNKTTSFTRIKAGVYKTEREKHKDLPSHYIYTACEDASERLDSFEKLKKRGRSYTEKPSVRRVTIHLDDHLWKFNLDTISISTKRSRILISPTFPKIFWRYYNTEWRIASEARFRLMKGNVVEFYVIFKRDEPKPYEPKAFIPVDLNENSVSVLINSKPLLLETNTKKITLGYEYRRKAITTGKSTKDREVRRKLKRLRERNKKVDIRRKLAKLIVKEAFESRSAIVLEDLPRRTPEHMIKDVKDKQLRLRIYRSAFSSMKNAIIEKAREFGVPVVLVNPSYTSTVCPIHGANIVYQLDGGDAPRVGVCEKGKEKWHRDVVALYNLARRAGDVSPVPLGSKESHDPPTLSGWLRAKSLHSIMNEHKMIEMKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-01-24" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X10</accession>
    <name>Q97X10_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative peptidoglycan binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90360">
        <property type="entry name" value="E90360"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X10"/>
    <dbReference type="STRING" id="273057.SSO1947"/>
    <dbReference type="PaxDb" id="273057-SSO1947"/>
    <dbReference type="EnsemblBacteria" id="AAK42140">
        <property type="protein sequence ID" value="AAK42140"/>
        <property type="gene ID" value="SSO1947"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1947"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2020"/>
    <dbReference type="eggNOG" id="arCOG04204">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1736500_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X10"/>
    <dbReference type="PhylomeDB" id="Q97X10"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010430">
        <property type="entry name" value="DUF1028"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014927">
        <property type="entry name" value="PG-bd_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39328">
        <property type="entry name" value="BLL2871 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39328:SF1">
        <property type="entry name" value="BLL2871 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06267">
        <property type="entry name" value="DUF1028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08823">
        <property type="entry name" value="PG_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative peptidoglycan binding" evidence="1">
        <location>
            <begin position="93"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="150" mass="17105" checksum="D3872E67C938C415" modified="2001-10-01" version="1">MHLGHSLEAMAKEAESKGKIYEKILRALKAGESKGGDRRGKQSAAIIVVKTVDKSEKEIDPLIVGKYVDLRVDDSQDPLKDLERLLDLWVATFIEEEMVNVKDYENQIRQALNKWGYNDLRTWVEMNNLEGKYTGDKIGKTVLKILLSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X35</accession>
    <name>Q97X35_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1395</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO9241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90357">
        <property type="entry name" value="B90357"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X35"/>
    <dbReference type="PaxDb" id="273057-SSO9241"/>
    <dbReference type="EnsemblBacteria" id="AAK42113">
        <property type="protein sequence ID" value="AAK42113"/>
        <property type="gene ID" value="SSO9241"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO9241"/>
    <dbReference type="eggNOG" id="arCOG02129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2730594_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X35"/>
    <dbReference type="PhylomeDB" id="Q97X35"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="3"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="71" mass="8253" checksum="4D7064E189CD6D59" modified="2001-10-01" version="1">MFYAHNVDFRVFADFLYLLRVRNSGNVVLILDNASFHKSSYVLATASRLNINLLFLPPYSLDLNRIEFGRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-12-21" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X60</accession>
    <name>Y1893_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Putative ABC transporter ATP-binding protein SSO1893</fullName>
            <ecNumber>7.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO1893</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90353">
        <property type="entry name" value="E90353"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992766.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X60"/>
    <dbReference type="SMR" id="Q97X60"/>
    <dbReference type="FunCoup" id="Q97X60">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1893"/>
    <dbReference type="PaxDb" id="273057-SSO1893"/>
    <dbReference type="EnsemblBacteria" id="AAK42084">
        <property type="protein sequence ID" value="AAK42084"/>
        <property type="gene ID" value="SSO1893"/>
    </dbReference>
    <dbReference type="GeneID" id="1453411"/>
    <dbReference type="KEGG" id="sso:SSO1893"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1949"/>
    <dbReference type="eggNOG" id="arCOG00188">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_86_7_2"/>
    <dbReference type="InParanoid" id="Q97X60"/>
    <dbReference type="PhylomeDB" id="Q97X60"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003633">
        <property type="entry name" value="Cobalt ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003642">
        <property type="entry name" value="Cobalt ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050095">
        <property type="entry name" value="ECF_ABC_transporter_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553:SF23">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553">
        <property type="entry name" value="HEAVY METAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000092161" description="Putative ABC transporter ATP-binding protein SSO1893">
        <location>
            <begin position="1"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter 1" evidence="2">
        <location>
            <begin position="6"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter 2" evidence="2">
        <location>
            <begin position="282"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="314"/>
            <end position="321"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="530" mass="59832" checksum="CE7D001546B8C6D3" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X73</accession>
    <name>Q97X73_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1880</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90351">
        <property type="entry name" value="F90351"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991420.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X73"/>
    <dbReference type="STRING" id="273057.SSO1880"/>
    <dbReference type="PaxDb" id="273057-SSO1880"/>
    <dbReference type="EnsemblBacteria" id="AAK42069">
        <property type="protein sequence ID" value="AAK42069"/>
        <property type="gene ID" value="SSO1880"/>
    </dbReference>
    <dbReference type="GeneID" id="1453396"/>
    <dbReference type="KEGG" id="sso:SSO1880"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1932"/>
    <dbReference type="eggNOG" id="arCOG00271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_5_0_2"/>
    <dbReference type="InParanoid" id="Q97X73"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050638">
        <property type="entry name" value="AA-Vitamin_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322:SF18">
        <property type="entry name" value="S-ADENOSYLMETHIONINE_S-ADENOSYLHOMOCYSTEINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="11"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="138"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="274" mass="29832" checksum="E5D23D2B368913B7" modified="2001-10-01" version="1">MQRTTISFLRWIVPLSLFWGFAFPLTKIIAYSASPMIISAFRIGIAAIFFLILGRGLSVGKKQFINGLMNFAFFLILINLGIGLSPDPGLVAVMIYTQPIFVLIIERLLGHKITPKGIVGVLLGVVGVISSATLSFNIGLVFGLLAGILWAGGTIYYSRNLAKEDIVKLNAFMTLTSLPIVLAFTPIDYYFRFSLVTLSLLLLLAILAQILGFYFWFNGVRELGTVYASTGSLLVPVMAYITSFAVLGVAPTLPQIIGSLITLFGVYLTITSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X83</accession>
    <name>Q97X83_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1234</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90350">
        <property type="entry name" value="D90350"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923632.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X83"/>
    <dbReference type="PaxDb" id="273057-SSO1871"/>
    <dbReference type="EnsemblBacteria" id="AAK42059">
        <property type="protein sequence ID" value="AAK42059"/>
        <property type="gene ID" value="SSO1871"/>
    </dbReference>
    <dbReference type="GeneID" id="1453386"/>
    <dbReference type="KEGG" id="sso:SSO1871"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1922"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075479_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X83"/>
    <dbReference type="PhylomeDB" id="Q97X83"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="319" mass="36858" checksum="36C002EECD1EF70E" modified="2001-10-01" version="1">MGVEGSTMARTLRHIPNLMYYPLPPIEDMPWREKWLTEIKPVLDTMDLERVLGEGALVYLKLLIVMVLYSCSYRDAVKMVNVNVVVAWFVGRKVGKSTLHDFVGRLYGVRKKLLEISFKLEEKCLPSYLPASAHLVDFMAWLVDSFLLDLPPGKRSVETFREKAELERREGNLERARKLLSLGRTKRRFEGRWTKKRGVSHYGLKAVAVISVSLFVRSITLKPANFSDKRFKSPLKGIKIADRGFSPSPTQLIAREKPFTTLRAHVEFFGTYLNAFWRPYGTTTWRNDVFLHVLGVIYNIKIFLAIQRRTPPERRAVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X96</accession>
    <name>Q97X96_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90348">
        <property type="entry name" value="G90348"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X96"/>
    <dbReference type="STRING" id="273057.SSO1858"/>
    <dbReference type="PaxDb" id="273057-SSO1858"/>
    <dbReference type="EnsemblBacteria" id="AAK42046">
        <property type="protein sequence ID" value="AAK42046"/>
        <property type="gene ID" value="SSO1858"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1858"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1908"/>
    <dbReference type="eggNOG" id="arCOG06009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1163847_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X96"/>
    <dbReference type="PhylomeDB" id="Q97X96"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="247" mass="27076" checksum="647A8D1F4B02B7E6" modified="2001-10-01" version="1">MINEVYNMGFNGIKFDTSVPGMIPWEIVVAYFILTPLVVYGLSRRLVKSSFTTIDFVYISIGGAFSVVWEFYVGSFIARFFPSSPFLGIGFWGRLFILLIVASLVRKPGVGAMSLTIYTLLADLFHYGFAGQPLYFIYEAFTYGLFIDAVIIATRGNLFNIRYSDSIGTSLKIKRVVLIAIEGAIIGILCAIPDPIFYLGFLNPLIHGAIVNWATIQFDVLASVPGDAIVGILGAFAGQRVARAVGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XG3</accession>
    <name>Q97XG3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1779</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90339">
        <property type="entry name" value="D90339"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923581.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XG3"/>
    <dbReference type="PaxDb" id="273057-SSO1779"/>
    <dbReference type="EnsemblBacteria" id="AAK41971">
        <property type="protein sequence ID" value="AAK41971"/>
        <property type="gene ID" value="SSO1779"/>
    </dbReference>
    <dbReference type="GeneID" id="1454750"/>
    <dbReference type="KEGG" id="sso:SSO1779"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1828"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XG3"/>
    <dbReference type="PhylomeDB" id="Q97XG3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="80"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37500" checksum="94C3781D96DBF192" modified="2001-10-01" version="1">MQVYETRFKTELKSLASYFIVNNLNVISQELDTRIADAAPFLLTGNRGKEYLKVVREGEKVMSQGERSFKFYPHVKVEGETTIVAVDETAITVGEKESRKVEGFQLYNGKRKGVKLRSVDLVFPLRLSLLEEIADLRSDTPSQFLMRAVSEVAQHLKIDYVVADAGFLNLKVIKEMPVKTVVGGKTNLKGFKELSNVSLTEKKCEVNDRVYVAYRVLKYNDLYYYEVIYVKEKPRHFIFVTNFNGDPYKLAELYRLRWQSEEGFKVRKARIRYVRKLTNKIFLYLYYTVLDSVWNLVNYLLFNFKSTKRKPLSFDSFVRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XN6</accession>
    <name>Q97XN6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1678</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90328">
        <property type="entry name" value="H90328"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923559.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XN6"/>
    <dbReference type="PaxDb" id="273057-SSO1678"/>
    <dbReference type="EnsemblBacteria" id="AAK41887">
        <property type="protein sequence ID" value="AAK41887"/>
        <property type="gene ID" value="SSO1678"/>
    </dbReference>
    <dbReference type="GeneID" id="1454666"/>
    <dbReference type="KEGG" id="sso:SSO1678"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1724"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XN6"/>
    <dbReference type="PhylomeDB" id="Q97XN6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="80"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37473" checksum="D4B91708973E298A" modified="2001-10-01" version="1">MQVYETGFKTELKSLASYFIVNNLNVISQELDTRIADAAPFLLTENRGKEYLKVVREGEKVMSQGERSFKFYPHVKVEGETTIVAVDETAITVGEKESRKVEGFQLYNGKRKGVKLRSVDLVFPLRLSLLEEIADLRSDTPSQFLMRAVSEVAQHLKIDYVVADAGFLNLKVIKEMPVKTVVGGKTNLKGFKELSNVSLTEKKCEVNDRVYVAYRVLKYNDLYYYEVIYVKEKPRHFIFVTNFNGDPYKLAELYRLRWQSEEGFKVRKARIRYVRKLTNKIFLYLYYTVLDSVWNLVNYLLFNFKSTKRKPLSFDSFVRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XX1</accession>
    <name>Q97XX1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8426</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90317">
        <property type="entry name" value="G90317"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XX1"/>
    <dbReference type="STRING" id="273057.SSO8426"/>
    <dbReference type="PaxDb" id="273057-SSO8426"/>
    <dbReference type="EnsemblBacteria" id="AAK41798">
        <property type="protein sequence ID" value="AAK41798"/>
        <property type="gene ID" value="SSO8426"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8426"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1623"/>
    <dbReference type="eggNOG" id="arCOG08539">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2695931_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XX1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="73" mass="8609" checksum="CD221C761AC2BAA6" modified="2001-10-01" version="1">MSMRAISALNVPLDTVFTLIKHHGRKRYKKLVQLWSKAKELVKGRSTGKIVVEMWTYLFTNTRAFYKWEFSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XY6</accession>
    <name>Q97XY6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1569</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90315">
        <property type="entry name" value="G90315"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XY6"/>
    <dbReference type="STRING" id="273057.SSO1569"/>
    <dbReference type="PaxDb" id="273057-SSO1569"/>
    <dbReference type="EnsemblBacteria" id="AAK41782">
        <property type="protein sequence ID" value="AAK41782"/>
        <property type="gene ID" value="SSO1569"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1569"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1605"/>
    <dbReference type="eggNOG" id="arCOG07305">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1933350_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XY6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="141" mass="16207" checksum="7EC60AE88A0B40AD" modified="2001-10-01" version="1">MKLGNRLRRLKLVNMREIYKYSIFSIIIMFLAFSFGLVRAMYDTVQNTFSTSILIFHIIFAGLTGGFGVYLYVLANKTGLLFPKFIGLGNLVSIGIAGFSGLSYLLTNYEWLTRLMLYGFEFSLALTSMLIGYLYCFMRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y24</accession>
    <name>Q97Y24_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Dihydrolipoamide dehydrogenase (PdhD-2)</fullName>
            <ecNumber evidence="12">1.8.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pdhD-2</name>
        <name evidence="12" type="ordered locus">SSO1524</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="12"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90311">
        <property type="entry name" value="D90311"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y24"/>
    <dbReference type="FunCoup" id="Q97Y24">
        <property type="interactions" value="249"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1524"/>
    <dbReference type="PaxDb" id="273057-SSO1524"/>
    <dbReference type="DNASU" id="1454526"/>
    <dbReference type="EnsemblBacteria" id="AAK41747">
        <property type="protein sequence ID" value="AAK41747"/>
        <property type="gene ID" value="SSO1524"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1524"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1564"/>
    <dbReference type="eggNOG" id="arCOG01068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_3_2"/>
    <dbReference type="InParanoid" id="Q97Y24"/>
    <dbReference type="PhylomeDB" id="Q97Y24"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000029">
        <property type="entry name" value="FAD-dependent pyridine nucleotide-disulphide oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0274">FAD</keyword>
    <keyword evidence="3 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5 9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="11">
        <location>
            <begin position="6"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="10">
        <location>
            <begin position="307"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK41747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="412" mass="45612" checksum="179DF9BA1D4C4DDE" modified="2001-10-01" version="1">MKNNIMKVVIIGAGPAGVYSALTLSKHAKVTLIEREEKLGGTCVLYGCIPTKSILSQLIISRQASNMSLNTLREYALTSINTISKSLEHLLNSHGIEVIHANGFLRSSMVHASNTSLAADKVLVSTGTRRERLGKVKFTEDLAYTNEDYNKVVIVGGDAGGIELGWMMKKLGKEVHLIDKNDLLLQNIDRTLSEIVTNFLSQIGIKLYLGKKVSKIDETSVTLEDNQKISGDAVFVTFGRKPNIEGFEEIPHEKYIYVDEYLRTQIPNIYAAGDIIGTFTAHEAIYAGIIAAKNMLGEKREFIVEGIPKVIYIYPQIAYVGTTNGNCVTFNTLNLTRTIVERESEGFLKICERDNRVIGAVAFMPYAEDVISLISVLIRYQISLKDTIDLVMPHPSYLEAITEALNMLQSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y40</accession>
    <name>Q97Y40_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposase ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1509</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90309">
        <property type="entry name" value="D90309"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y40"/>
    <dbReference type="STRING" id="273057.SSO1509"/>
    <dbReference type="PaxDb" id="273057-SSO1509"/>
    <dbReference type="EnsemblBacteria" id="AAK41731">
        <property type="protein sequence ID" value="AAK41731"/>
        <property type="gene ID" value="SSO1509"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1509"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1549"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074024_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y40"/>
    <dbReference type="PhylomeDB" id="Q97Y40"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025471">
        <property type="entry name" value="DUF4322"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033541">
        <property type="entry name" value="transpos_ISH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14210">
        <property type="entry name" value="DUF4322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4322" evidence="3">
        <location>
            <begin position="14"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="108"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="364" mass="42043" checksum="429F3B8A03CED02C" modified="2001-10-01" version="1">MRKPVLSRGEDKVITPGLPHQNNIQQVGYKLLSMLSFKGRKAEEVSRVLVSACLWNDSVESKSKGYNVSPQTVRNYVEEQGTEVIEKLLESMRRISMEILKGVKEVDISIDWTTKTWYGKPVEGLGSSAKGNSWNYATATTKYQNMVLLLAFVPQVNGMSKDEIVKLLMEQIVGMGLKVGLVTLDAGFYTVEVLKFISQFKFVIGVPVGDVKIYEEFDGEYTTNSKRHKKEEQVKFRLLVYGKEIVKKRKKTVVYFARATNLDLPKREVLKLYNKVRSPIETSYRNIKAFLPFTSSTKFIFRELIFVLAMIFYSLYTVFKNVMTREEFRLLLILCFLDDLSDLKDFIFNLEKTLINTIDLFLWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y52</accession>
    <name>Q97Y52_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VapB-type antitoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1494</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90307">
        <property type="entry name" value="H90307"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y52"/>
    <dbReference type="PaxDb" id="273057-SSO1494"/>
    <dbReference type="EnsemblBacteria" id="AAK41719">
        <property type="protein sequence ID" value="AAK41719"/>
        <property type="gene ID" value="SSO1494"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1494"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1533"/>
    <dbReference type="eggNOG" id="arCOG02217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175270_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y52"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039709">
        <property type="entry name" value="VapB3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42244">
        <property type="entry name" value="ANTITOXIN VAPB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42244:SF2">
        <property type="entry name" value="ANTITOXIN VAPB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="12195" checksum="812880EB7C1C5175" modified="2001-10-01" version="1">MVIKNATILIYVPEIETFIFPVYVYYNMKTVYSLRLDKELREEMEKYNVKWNEEIESFIRKRIEELKKEEILKKINEILQTMPETKSNSADLVREDRDNN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y92</accession>
    <name>CAS5A_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR system aCascade subunit Cas5 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cas5a</name>
        <name type="ordered locus">SSO1441</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="J. Biol. Chem." volume="286" first="21643" last="21656">
            <title>Structural and functional characterization of an archaeal clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for antiviral defense (CASCADE).</title>
            <authorList>
                <person name="Lintner N.G."/>
                <person name="Kerou M."/>
                <person name="Brumfield S.K."/>
                <person name="Graham S."/>
                <person name="Liu H."/>
                <person name="Naismith J.H."/>
                <person name="Sdano M."/>
                <person name="Peng N."/>
                <person name="She Q."/>
                <person name="Copie V."/>
                <person name="Young M.J."/>
                <person name="White M.F."/>
                <person name="Lawrence C.M."/>
            </authorList>
            <dbReference type="PubMed" id="21507944"/>
            <dbReference type="DOI" id="10.1074/jbc.M111.238485"/>
        </citation>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH CSA2</scope>
        <source>
            <strain>ATCC 35091 / DSM 1616 / JCM 8930 / NBRC 15331 / P1</strain>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the aCascade ribonucleoprotein complex, minimally composed of Csa2 and Cas5a, which binds crRNA. Other possible components of aCascade in strain P1 are Cas6b (SSO1437) and Csa5 (SSO1443), while SSO1399, Cas5b (SSO1400) and SSO1401 have sometimes been seen weakly associated. Csa2 is probably the major RNA-binding subunit. The Csa2-Cas5a-crRNA complex also binds target DNA homologous to crRNA, probably forming an R-loop. Purified aCascade forms a filament about 6 nm in width.</text>
    </comment>
    <comment type="miscellaneous">
        <text>Cloning was done with strain P2, while interaction experiments were done with genes cloned from strain P2 but over-expressed in P1. The aCascade complex was purified from strain P1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR-associated protein Cas5 family. Subtype I-A/Apern subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90302">
        <property type="entry name" value="C90302"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923406.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9CP1">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="G=1-240"/>
    </dbReference>
    <dbReference type="PDB" id="9CP2">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.94 A"/>
        <property type="chains" value="G=1-240"/>
    </dbReference>
    <dbReference type="PDB" id="9CP3">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.94 A"/>
        <property type="chains" value="G=1-240"/>
    </dbReference>
    <dbReference type="PDB" id="9CRO">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="H=1-240"/>
    </dbReference>
    <dbReference type="PDB" id="9CRP">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.20 A"/>
        <property type="chains" value="G=1-240"/>
    </dbReference>
    <dbReference type="PDB" id="9CRQ">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.07 A"/>
        <property type="chains" value="G=1-240"/>
    </dbReference>
    <dbReference type="PDBsum" id="9CP1"/>
    <dbReference type="PDBsum" id="9CP2"/>
    <dbReference type="PDBsum" id="9CP3"/>
    <dbReference type="PDBsum" id="9CRO"/>
    <dbReference type="PDBsum" id="9CRP"/>
    <dbReference type="PDBsum" id="9CRQ"/>
    <dbReference type="AlphaFoldDB" id="Q97Y92"/>
    <dbReference type="EMDB" id="EMD-45796"/>
    <dbReference type="EMDB" id="EMD-45797"/>
    <dbReference type="EMDB" id="EMD-45798"/>
    <dbReference type="EMDB" id="EMD-45873"/>
    <dbReference type="EMDB" id="EMD-45874"/>
    <dbReference type="EMDB" id="EMD-45875"/>
    <dbReference type="SMR" id="Q97Y92"/>
    <dbReference type="STRING" id="273057.SSO1441"/>
    <dbReference type="PaxDb" id="273057-SSO1441"/>
    <dbReference type="EnsemblBacteria" id="AAK41674">
        <property type="protein sequence ID" value="AAK41674"/>
        <property type="gene ID" value="SSO1441"/>
    </dbReference>
    <dbReference type="GeneID" id="1454453"/>
    <dbReference type="GeneID" id="27427809"/>
    <dbReference type="KEGG" id="sso:SSO1441"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1470"/>
    <dbReference type="eggNOG" id="arCOG02670">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154391_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y92"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09753">
        <property type="entry name" value="Cas5_I-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013422">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010153">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5a-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053725">
        <property type="entry name" value="CRISPR_Cas5_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01874">
        <property type="entry name" value="cas_cas5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02593">
        <property type="entry name" value="CRISPR_cas5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000417892" description="CRISPR system aCascade subunit Cas5 1">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="21507944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="240" mass="27397" checksum="6002773E6BD6A2FB" modified="2001-10-01" version="1">MIYSKVFLKLHWGFSVVKPLAAKAKPGFYLPPPTTLIGALSYGKFRGVDNINLGNVYGSPAYNFRNIMATARLESEGVYTEDIIRNVISYFQRKERRENPRYIYGVIPTGKVYIPNGRLVVVYVTDSISKEELEKLCWSITRIGCKECLASVENVEVGEAKKVSGRVKTRYYFRDTVKVVGRKEFLEYVTFWEENGYIWGKEGSPVRYILPITTYPLASKEVEVEAKEAYEVGGEYVVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YA9</accession>
    <name>Q97YA9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator TrmB N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90300">
        <property type="entry name" value="B90300"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923389.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YA9"/>
    <dbReference type="STRING" id="273057.SSO1423"/>
    <dbReference type="PaxDb" id="273057-SSO1423"/>
    <dbReference type="EnsemblBacteria" id="AAK41657">
        <property type="protein sequence ID" value="AAK41657"/>
        <property type="gene ID" value="SSO1423"/>
    </dbReference>
    <dbReference type="GeneID" id="1454436"/>
    <dbReference type="KEGG" id="sso:SSO1423"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1449"/>
    <dbReference type="eggNOG" id="arCOG01446">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1363705_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YA9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="123"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="200" mass="23104" checksum="F7EE43DACC788ABB" modified="2001-10-01" version="1">MVYLNYEIENYTRNVKRVIVCGDVKGFRLRVNRVEVIDNCLSLDVEGIAESIARRIGNSKDSYVVLLNGNSNKFAAAMFYLFSIICKRVKFVIYDRERVELDGDVFRKVEIDDKMITVLGEIMNGRYKIEEIEKDTGISKATISRVRRKLEEMGLVKKVDGGKYCISERGKIVVYDGGFFRNVKKGGRYYESKTLYTERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YD7</accession>
    <name>Q97YD7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">One of two inversely orientated ORFs in ISC1043</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1387</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99296">
        <property type="entry name" value="A99296"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991621.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YD7"/>
    <dbReference type="STRING" id="273057.SSO1387"/>
    <dbReference type="PaxDb" id="273057-SSO1387"/>
    <dbReference type="EnsemblBacteria" id="AAK41624">
        <property type="protein sequence ID" value="AAK41624"/>
        <property type="gene ID" value="SSO1387"/>
    </dbReference>
    <dbReference type="GeneID" id="44089139"/>
    <dbReference type="KEGG" id="sso:SSO1387"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1394"/>
    <dbReference type="eggNOG" id="arCOG07762">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179762_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YD7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="101" mass="12314" checksum="CE3A2256E4D24B2D" modified="2001-10-01" version="1">MRADKYYKSTLDDFPDSEVYLIPKSNATIKGGKRWREMILRFMRNPLEYLEEYFKRERAESLIFIKRRTRWIINKVREERIHLTILARVAIHNLLWSKALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YG4</accession>
    <name>Q97YG4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90292">
        <property type="entry name" value="C90292"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923351.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YG4"/>
    <dbReference type="STRING" id="273057.SSO1359"/>
    <dbReference type="PaxDb" id="273057-SSO1359"/>
    <dbReference type="EnsemblBacteria" id="AAK41594">
        <property type="protein sequence ID" value="AAK41594"/>
        <property type="gene ID" value="SSO1359"/>
    </dbReference>
    <dbReference type="GeneID" id="1454373"/>
    <dbReference type="KEGG" id="sso:SSO1359"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1365"/>
    <dbReference type="eggNOG" id="arCOG05999">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1551727_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YG4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="176" mass="20718" checksum="E266C997B2BFFB76" modified="2001-10-01" version="1">MRGRKITLMLITSMLLNILVALLPSYWWYYSAGGMVTIKDSLFSFYLEFLGRTLEIGTIINYILFAFRFYVISVSLYYIYLALKKEIIKHYLLITWVSYLYILDPLIFYLLFNNVVNYFTPVKYPLFIIGSENMSIVYKNVIVTVLVESYPTIYYWIALFAGTFNLISRIITGRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YH5</accession>
    <name>Q97YH5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Conjugative plasmid protein (PARN3)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90290">
        <property type="entry name" value="G90290"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923339.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YH5"/>
    <dbReference type="PaxDb" id="273057-SSO1346"/>
    <dbReference type="EnsemblBacteria" id="AAK41582">
        <property type="protein sequence ID" value="AAK41582"/>
        <property type="gene ID" value="SSO1346"/>
    </dbReference>
    <dbReference type="GeneID" id="1454361"/>
    <dbReference type="KEGG" id="sso:SSO1346"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1352"/>
    <dbReference type="eggNOG" id="arCOG07296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1363703_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YH5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="25233" checksum="34E8F6474C109D87" modified="2001-10-01" version="1">MMKRKFDLKKDVIIALNPLQTRYQLFSERVFQPLYSATNDGIYRMERLKRTGIGLGLTLAELGVGVLIIFLGIFIYFTIYFTYQYIYIPPLAHYPLAPEIIVYAIPFAIPIGIFIGIIVALVFGIVKLVKIIRKLSRKINIAEGELVVPWVSVRNIVVTNVRQENIANRPSITLNIISPVYKEIGDWHVLTTDGRDITIPNVDDPFNKLNYVKNRFNLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YI4</accession>
    <name>Q97YI4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Permease, sugar transport protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1338</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90289">
        <property type="entry name" value="H90289"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YI4"/>
    <dbReference type="FunCoup" id="Q97YI4">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1338"/>
    <dbReference type="PaxDb" id="273057-SSO1338"/>
    <dbReference type="EnsemblBacteria" id="AAK41575">
        <property type="protein sequence ID" value="AAK41575"/>
        <property type="gene ID" value="SSO1338"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1338"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1343"/>
    <dbReference type="eggNOG" id="arCOG02691">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_5_2"/>
    <dbReference type="InParanoid" id="Q97YI4"/>
    <dbReference type="PhylomeDB" id="Q97YI4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045:SF1">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="252"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="5">
        <location>
            <begin position="1"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="356" mass="39557" checksum="76CE153D1F2EC55D" modified="2001-10-01" version="1">MLFLLTIVIGYFARPLGGIFFGHYGDKIGRKRIWFVSLLGMGIATTLIGFLPTYNQVGIFATILLILLRILQGFFLAGEWGGGMTLVNEFSPSNLRGLMSGVQQGGAALGLIFAVIASEIASSLAPGSAFATYGWRIMFWFGVIPLIIALTVRWKVGESIEWLTKVADKPEKIPIATVFKKWWKLVIIAAVVLFSSGSIYYGIIAYMSTFFELYTHLSIYEIDNIVLATNLVWLFLSPLTGYLSDILKMRKTLIAILGFIIGILVYPIINVLYTSSYILSILAGMLLGFLFAFQYSIFPAWLSENINTNVRYSYIAFSINLGVALSSFAPYIVTALGLIFHNPVIGLQFLIYLLQY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-08-29" modified="2025-06-18" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YJ9</accession>
    <name>ILVC2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Putative ketol-acid reductoisomerase 2</fullName>
            <ecNumber>1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Acetohydroxy-acid isomeroreductase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Alpha-keto-beta-hydroxylacyl reductoisomerase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ilvC2</name>
        <name type="synonym">ilvC-2</name>
        <name type="ordered locus">SSO1322</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Ser-107 is present instead of the conserved His which is expected to be an active site residue.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99288">
        <property type="entry name" value="A99288"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923317.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="6JCV">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.92 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-333"/>
    </dbReference>
    <dbReference type="PDB" id="6JCW">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.04 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-333"/>
    </dbReference>
    <dbReference type="PDB" id="6JCZ">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.35 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-333"/>
    </dbReference>
    <dbReference type="PDB" id="6JD1">
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        <property type="resolution" value="3.38 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-333"/>
    </dbReference>
    <dbReference type="PDB" id="6JD2">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.53 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-333"/>
    </dbReference>
    <dbReference type="PDB" id="6KOU">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.43 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-333"/>
    </dbReference>
    <dbReference type="PDB" id="6KPA">
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        <property type="resolution" value="2.75 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-333"/>
    </dbReference>
    <dbReference type="PDB" id="6KPE">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.83 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-333"/>
    </dbReference>
    <dbReference type="PDB" id="6KPH">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.41 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-333"/>
    </dbReference>
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        <property type="resolution" value="2.43 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-333"/>
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        <property type="resolution" value="2.56 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-333"/>
    </dbReference>
    <dbReference type="PDB" id="6KPK">
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        <property type="resolution" value="2.30 A"/>
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    </dbReference>
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        <property type="method" value="EM"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-333"/>
    </dbReference>
    <dbReference type="PDB" id="6KQ8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-333"/>
    </dbReference>
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        <property type="resolution" value="2.54 A"/>
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    </dbReference>
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        <property type="resolution" value="2.17 A"/>
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    </dbReference>
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        <property type="resolution" value="2.52 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-333"/>
    </dbReference>
    <dbReference type="PDBsum" id="6JCV"/>
    <dbReference type="PDBsum" id="6JCW"/>
    <dbReference type="PDBsum" id="6JCZ"/>
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    <dbReference type="PDBsum" id="6KPH"/>
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    <dbReference type="PDBsum" id="6KPJ"/>
    <dbReference type="PDBsum" id="6KPK"/>
    <dbReference type="PDBsum" id="6KQ4"/>
    <dbReference type="PDBsum" id="6KQ8"/>
    <dbReference type="PDBsum" id="6KQJ"/>
    <dbReference type="PDBsum" id="6KQK"/>
    <dbReference type="PDBsum" id="6KQO"/>
    <dbReference type="AlphaFoldDB" id="Q97YJ9"/>
    <dbReference type="EMDB" id="EMD-9798"/>
    <dbReference type="EMDB" id="EMD-9799"/>
    <dbReference type="EMDB" id="EMD-9800"/>
    <dbReference type="EMDB" id="EMD-9801"/>
    <dbReference type="SMR" id="Q97YJ9"/>
    <dbReference type="FunCoup" id="Q97YJ9">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1322"/>
    <dbReference type="PaxDb" id="273057-SSO1322"/>
    <dbReference type="EnsemblBacteria" id="AAK41560">
        <property type="protein sequence ID" value="AAK41560"/>
        <property type="gene ID" value="SSO1322"/>
    </dbReference>
    <dbReference type="GeneID" id="1454339"/>
    <dbReference type="KEGG" id="sso:SSO1322"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1324"/>
    <dbReference type="eggNOG" id="arCOG04465">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033821_0_1_2"/>
    <dbReference type="InParanoid" id="Q97YJ9"/>
    <dbReference type="PhylomeDB" id="Q97YJ9"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001099">
        <property type="entry name" value="Putative ketol-acid reductoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371:SF1">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="KARI_C"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="KARI_N"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151401" description="Putative ketol-acid reductoisomerase 2">
        <location>
            <begin position="1"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="1">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="2">
        <location>
            <begin position="183"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="4"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="12"/>
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        </location>
    </feature>
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    </feature>
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    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="248"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="255"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="287"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="6JD1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="6KPA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="6KPH"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="6KPJ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="6KQK"/>
        </source>
    </evidence>
    <sequence length="333" mass="37185" checksum="D37083F704B95CA0" modified="2001-08-29" version="1">MDKTVLDANLDPLKGKTIGVIGYGNQGRVQATIMRENGLNVIVGNVKDKYYELAKKEGFEVYEIDEAVRRSDVALLLIPDEVMKEVYEKKIAPVLQGKKEFVLDFASGYNVAFGLIRPPKSVDTIMVAPRMVGEGIMDLHKQGKGYPVLLGVKQDASGKAWDYAKAIAKGIGAIPGGIAVISSFEEEALLDLMSEHTWVPILFGAIKACYDIAVKEYGVSPEAALLEFYASGELAEIARLIAEEGIFNQMVHHSTTSQYGTLTRMFKYYDVVRRIVENEAKYIWDGSFAKEWSLEQQAGYPVFYRLWELATQSEMAKAEKELYKLLGRKVKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YM1</accession>
    <name>Q97YM1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Second ORF in transposon ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1302</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90285">
        <property type="entry name" value="C90285"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YM1"/>
    <dbReference type="PaxDb" id="273057-SSO1302"/>
    <dbReference type="EnsemblBacteria" id="AAK41538">
        <property type="protein sequence ID" value="AAK41538"/>
        <property type="gene ID" value="SSO1302"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1302"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1302"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YM1"/>
    <dbReference type="PhylomeDB" id="Q97YM1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="14"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="229" mass="27164" checksum="B99356D7C3122EC9" modified="2001-10-01" version="1">MGEKEQEKAEGFLLYNWKRKGVKMRSLDLVYPLRLPLLVEIADLRSDSPSQFLLRSVREVSQYMEIDYVVADAGFLNLGVIKEMPVKTIVRGKSNLKGSKELSNVPLVEKRYEVKDKVYVAYRVLEFEGLYYYDVVYVKGKPRHFIFVTNFEGDPYELAELYRLRWQVEEGFKVRKARIRYVRKLSNKIFLFLYYTVLDSAWNLVNHLLFNFKSTCKKVLYFDSFVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YR2</accession>
    <name>Q97YR2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Galactarate dehydratase, putative (GarD)</fullName>
            <ecNumber evidence="5">4.2.1.42</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">garD</name>
        <name evidence="5" type="ordered locus">SSO1259</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UxaA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.42" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90280">
        <property type="entry name" value="B90280"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991889.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YR2"/>
    <dbReference type="FunCoup" id="Q97YR2">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1259"/>
    <dbReference type="PaxDb" id="273057-SSO1259"/>
    <dbReference type="EnsemblBacteria" id="AAK41497">
        <property type="protein sequence ID" value="AAK41497"/>
        <property type="gene ID" value="SSO1259"/>
    </dbReference>
    <dbReference type="GeneID" id="1454276"/>
    <dbReference type="KEGG" id="sso:SSO1259"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1254"/>
    <dbReference type="eggNOG" id="arCOG05317">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029189_1_0_2"/>
    <dbReference type="InParanoid" id="Q97YR2"/>
    <dbReference type="PhylomeDB" id="Q97YR2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008867">
        <property type="term" value="F:galactarate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019698">
        <property type="term" value="P:D-galacturonate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048332">
        <property type="entry name" value="GD_AH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007392">
        <property type="entry name" value="GD_AH_second"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052172">
        <property type="entry name" value="UxaA_altronate/galactarate_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30536:SF5">
        <property type="entry name" value="ALTRONATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30536">
        <property type="entry name" value="ALTRONATE/GALACTARATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20629">
        <property type="entry name" value="GD_AH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04295">
        <property type="entry name" value="GD_AH_second"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-galactarate/Altronate dehydratase second" evidence="3">
        <location>
            <begin position="8"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="D-galactarate/Altronate dehydratase C-terminal" evidence="4">
        <location>
            <begin position="142"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="387" mass="41489" checksum="8E20F69526B28ACC" modified="2001-10-01" version="1">MEKPIIRGYIRENGSVGVRNYVAVIPVDDLSNSASIGVSKLIRGTVAIPHPYGRLQFGKDLDLLFHVLSGTGANPNVAAAIVIGIEENWANKVADKIAETGKPVEVFPVEGNGDLKVIEKASRKAKEFVQEASEKQRTEVDLSSLVLSIKCGESDTTSGLASNPATGYAVDKLIDYGSTVMFGETSELTGAEDIVASRIQDPVLRDKFMRIYNEYVAFIESQGVDLLGSQPTEGNIKGGLSTIEEKALGNIQKLGTKPITCVLDYLDPLGKGSSRLCFVNTSSAAAEAVTLFAAKGSVLHLFTTGQGNVVGHPIIPVVKISANPKTIATMSEHIDVDVSDLLQLKVSLKEAGERIFNYTIRVANGRLTSAEILQHEEFSPIKLYISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YU5</accession>
    <name>Q97YU5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90275">
        <property type="entry name" value="C90275"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YU5"/>
    <dbReference type="STRING" id="273057.SSO1210"/>
    <dbReference type="PaxDb" id="273057-SSO1210"/>
    <dbReference type="EnsemblBacteria" id="AAK41458">
        <property type="protein sequence ID" value="AAK41458"/>
        <property type="gene ID" value="SSO1210"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1210"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1209"/>
    <dbReference type="eggNOG" id="arCOG08390">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145191_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YU5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="88"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="151" mass="18079" checksum="3C78878336FA2AE2" modified="2001-10-01" version="1">MLYNIVLMRQIEQINKVHTRYRREIRVEITKTKNKYKSSTSMIIMLEEWIKQLSIALDKFVHGEQLTEDERIAVASIIYATIKYLDNFDKCNDELKEIEEKCKKKLEEVQKELTDLHKQLEEKEEEVDEKLKTLDNLVKIIESNPRLLPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z62</accession>
    <name>Q97Z62_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial transposase ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90259">
        <property type="entry name" value="F90259"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z62"/>
    <dbReference type="PaxDb" id="273057-SSO1070"/>
    <dbReference type="EnsemblBacteria" id="AAK41333">
        <property type="protein sequence ID" value="AAK41333"/>
        <property type="gene ID" value="SSO1070"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1070"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1066"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1302673_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z62"/>
    <dbReference type="PhylomeDB" id="Q97Z62"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="1">
        <location>
            <begin position="106"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="227" mass="26510" checksum="2ADE42AC1A482035" modified="2001-10-01" version="1">MVNKTKDTYNPLRELVSLYLFMKDAQTKQENRLRRALFLVNDEEKLSEERLEKFSKGDFSDVKLYQLEYTGTVLREIQVLARILLQTREELKEVGKMIESNVPQDHVLLTIPGIGKLAAGIIIGIVGDIRRFPRPESFVAYCGLDPVVERSGRIEVRKGISKRGNKYLRSLFYFLAETQYSRNPTLLKFYETHKDKLKGKKLYTALARKLARIVWSVWYNNKPYEVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z92</accession>
    <name>CCA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CCA-adding enzyme</fullName>
            <ecNumber evidence="1">2.7.7.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">CCA tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA CCA-pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA adenylyl-/cytidylyl- transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-NT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cca</name>
        <name type="ordered locus">SSO1039</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a tRNA with a 3' CCA end + 2 CTP + ATP = a tRNA with a 3' CCACCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:76235"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="Rhea" id="RHEA-COMP:18655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="ChEBI" id="CHEBI:195187"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:76236"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">A single active site specifically recognizes both ATP and CTP and is responsible for their addition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90255">
        <property type="entry name" value="F90255"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989193.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z92"/>
    <dbReference type="SMR" id="Q97Z92"/>
    <dbReference type="FunCoup" id="Q97Z92">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1039"/>
    <dbReference type="PaxDb" id="273057-SSO1039"/>
    <dbReference type="EnsemblBacteria" id="AAK41301">
        <property type="protein sequence ID" value="AAK41301"/>
        <property type="gene ID" value="SSO1039"/>
    </dbReference>
    <dbReference type="GeneID" id="1454080"/>
    <dbReference type="GeneID" id="44129968"/>
    <dbReference type="KEGG" id="sso:SSO1039"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1033"/>
    <dbReference type="eggNOG" id="arCOG04249">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044679_1_0_2"/>
    <dbReference type="InParanoid" id="Q97Z92"/>
    <dbReference type="PhylomeDB" id="Q97Z92"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160016">
        <property type="term" value="F:CCACCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05400">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.30">
        <property type="entry name" value="CCA tRNA nucleotidyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01264">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048833">
        <property type="entry name" value="CAA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008229">
        <property type="entry name" value="CCA-adding_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042090">
        <property type="entry name" value="CCA_tRNA_nucleotrans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006116">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015329">
        <property type="entry name" value="tRNA_NucTransf2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03671">
        <property type="entry name" value="cca_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643">
        <property type="entry name" value="CCA-ADDING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643:SF1">
        <property type="entry name" value="CCA-ADDING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21133">
        <property type="entry name" value="CAA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09249">
        <property type="entry name" value="tRNA_NucTransf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005335">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0692">RNA repair</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000139083" description="CCA-adding enzyme">
        <location>
            <begin position="1"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01264"/>
        </source>
    </evidence>
    <sequence length="412" mass="47951" checksum="11FAE3F13168991B" modified="2001-09-26" version="1">MIEEEVLKIIKPTEEDKKGIEKVLEIIRERLNKLDFEVEGSFRKGTWLRQDTDIDVFVFYPKDVGKEYLERNALNDIINRIKDLDYTLAYAEHPYVIVNINNVEVDIVPALRVESGDKAITAVDRTPFHTKYVTSHLDERGKDEVRLLKRFMKGIGVYGAELKVQGFSGYATELLIIYYGNFRKVLEEASKWKHPIKIELTKPMKIFSEPLIIPDPVDPKRNVTAAVSLKNIATFSIAAKYYLKNPSIEFFFPSKKVEEKVKGDVLILRLNLDEKSSEDIVWGQIKRSVNKIERALKQYGFRVIDVQAWGDTNNITIAVQLESKNIGQYYLNIGPQYYSGTIEDFIQKNDNIWVGEDGRLYSIKERKEYDAETIAKKNIVLKVKYNIESYWLQNTEDQQIMKFLRKTPTWLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="142" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z96</accession>
    <name>Q97Z96_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter, ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1034</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90255">
        <property type="entry name" value="B90255"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989200.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z96"/>
    <dbReference type="STRING" id="273057.SSO1034"/>
    <dbReference type="PaxDb" id="273057-SSO1034"/>
    <dbReference type="EnsemblBacteria" id="AAK41297">
        <property type="protein sequence ID" value="AAK41297"/>
        <property type="gene ID" value="SSO1034"/>
    </dbReference>
    <dbReference type="GeneID" id="7806883"/>
    <dbReference type="KEGG" id="sso:SSO1034"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1027"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q97Z96"/>
    <dbReference type="PhylomeDB" id="Q97Z96"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903607">
        <property type="term" value="P:cytochrome c biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051782">
        <property type="entry name" value="ABC_Transporter_VariousFunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939:SF1">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="204" mass="23556" checksum="430A10EFA9ACD0A8" modified="2001-10-01" version="1">MSEIVKVDVFKKFKNFELKVNFSVNEGEKVLIQGPNGSGKTTLLYLIYGVLNPDSGYVRVFNKNPTVELRKSEMYMLEEYLIFLENASLKENLEFITSLRDFNINKTEELINKFDLPLYKKSYQLSSGQRRLFKLALAFSAEAKLLLLDEPTSNLDIENSKAVKDMIREYSGTIIFASHDLLLKESCDKTIYLRTGKIERIEIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z99</accession>
    <name>Y1031_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized solute-binding protein SSO1031</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO1031</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the bacterial solute-binding protein 1 family. WtpA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90254">
        <property type="entry name" value="G90254"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989206.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z99"/>
    <dbReference type="SMR" id="Q97Z99"/>
    <dbReference type="FunCoup" id="Q97Z99">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1031"/>
    <dbReference type="PaxDb" id="273057-SSO1031"/>
    <dbReference type="EnsemblBacteria" id="AAK41294">
        <property type="protein sequence ID" value="AAK41294"/>
        <property type="gene ID" value="SSO1031"/>
    </dbReference>
    <dbReference type="GeneID" id="1454073"/>
    <dbReference type="KEGG" id="sso:SSO1031"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1023"/>
    <dbReference type="eggNOG" id="arCOG00219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_793701_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z99"/>
    <dbReference type="PhylomeDB" id="Q97Z99"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030973">
        <property type="term" value="F:molybdate ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13540">
        <property type="entry name" value="PBP2_ModA_WtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050682">
        <property type="entry name" value="ModA/WtpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30632">
        <property type="entry name" value="MOLYBDATE-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30632:SF16">
        <property type="entry name" value="MOLYBDATE_TUNGSTATE-BINDING PROTEIN WTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13531">
        <property type="entry name" value="SBP_bac_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000159726" description="Uncharacterized solute-binding protein SSO1031">
        <location>
            <begin position="45"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="402" mass="44294" checksum="94E381C14582A20A" modified="2001-10-01" version="1" precursor="true">MLEKNLLPEILLAIHMPLNKGLTRVKAIVIIIVVIIAVIAGVVGYYLINHPSNSVTTSSSSTTTSSSLSSTSISSSTTNITSSQGITVFVAGAYLAILNYLADQFQNATEIPVHVVGSGSFALASQIASQTPVPANVFIPVAYIQAVELTGSRNPGWAIAFLSDQMTIVYSNYTTKSPYWSQLYSNYTMAMETNNTKYWYNFFYLLTTRFSLGIANPNTDPEGLYAYLILQMASYLYANHNISYFVHLVKANPNVKVAPSTANYVAPLKAGTLDFTFSYVSYAVSQGLEYLKLPPWLSFGYYPNETTWYSQFAYNISVNGQTLTIHGNPVYLYITIPLNASNIQTAYQFIGFVLGHESQLTRFNVIPIQPALLYNETSNIPQPILNLLKSGELKYAGNFSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZA9</accession>
    <name>Q97ZA9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Formate hydrogenlyase subunit 3 (HycC)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hycC</name>
        <name evidence="4" type="ordered locus">SSO1021</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90253">
        <property type="entry name" value="E90253"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZA9"/>
    <dbReference type="STRING" id="273057.SSO1021"/>
    <dbReference type="PaxDb" id="273057-SSO1021"/>
    <dbReference type="EnsemblBacteria" id="AAK41284">
        <property type="protein sequence ID" value="AAK41284"/>
        <property type="gene ID" value="SSO1021"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1021"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1012"/>
    <dbReference type="eggNOG" id="arCOG01537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_8_1_2"/>
    <dbReference type="InParanoid" id="Q97ZA9"/>
    <dbReference type="PhylomeDB" id="Q97ZA9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050616">
        <property type="entry name" value="CPA3_Na-H_Antiporter_A"/>
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        <property type="entry name" value="ND/Mrp_TM"/>
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        <property type="entry name" value="PRK06459.1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR43373">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT"/>
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    <dbReference type="PANTHER" id="PTHR43373:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT A"/>
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    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
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        <location>
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        <location>
            <begin position="1"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="621" mass="69446" checksum="7FE96607E5064A50" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZC7</accession>
    <name>Q97ZC7_SACS2</name>
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        <recommendedName>
            <fullName evidence="1">DUF302 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0995</name>
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    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
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            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
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            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="C90251"/>
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    </dbReference>
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        <property type="entry name" value="SLR5037 PROTEIN"/>
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    </dbReference>
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    </dbReference>
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            <end position="94"/>
        </location>
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        <source>
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        </source>
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        <source>
            <dbReference type="EMBL" id="AAK41266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="123" mass="14057" checksum="D926BE34E232AB34" modified="2001-10-01" version="1">MFNVKECKLGFNECVKTLIERIKSSGAEVFAIIDHSENARKVGLSLEPTIVIYFGNPSVGTLLMLHNRHVAYELPLRFLVWSENNVVKIGYRKPSEVGEEYDIRNKELLDKMDKFVEGLLSNL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2026-01-28" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZH4</accession>
    <name>RS30_SACS2</name>
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        <recommendedName>
            <fullName evidence="2">Small ribosomal subunit protein eS30</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>30S ribosomal protein S30</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps30e</name>
        <name evidence="4" type="ordered locus">SSO6817</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7 8 9" key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Located in the mRNA entry channel, it binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the 30S small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eS30 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="G90244"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="e=1-52"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="e=1-52"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="e=1-52"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="e=1-52"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="e=1-52"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="e=1-52"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="e=1-52"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="e=1-52"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
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    <dbReference type="PDBsum" id="9FRK"/>
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    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
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    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
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    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q97ZH4"/>
    <dbReference type="FunCoup" id="Q97ZH4">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO6817"/>
    <dbReference type="PaxDb" id="273057-SSO6817"/>
    <dbReference type="EnsemblBacteria" id="AAK41214">
        <property type="protein sequence ID" value="AAK41214"/>
        <property type="gene ID" value="SSO6817"/>
    </dbReference>
    <dbReference type="GeneID" id="42364145"/>
    <dbReference type="KEGG" id="sso:SSO6817"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.934"/>
    <dbReference type="eggNOG" id="arCOG04293">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_209471_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZH4"/>
    <dbReference type="PhylomeDB" id="Q97ZH4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006846">
        <property type="entry name" value="Ribosomal_eS30"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006817">
        <property type="entry name" value="PRK09336.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04758">
        <property type="entry name" value="Ribosomal_S30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000463553" description="Small ribosomal subunit protein eS30">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000312" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="52" mass="6046" checksum="A9EB9D3E4E7E744E" modified="2001-10-01" version="1">MPSHGSLTKAGKVRSQTPKIQPKEKHKEVPRVRNRKEYEKRVVKARQQAPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZH9</accession>
    <name>Q97ZH9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Vitamin B12-dependent ribonucleotide reductase</fullName>
            <ecNumber evidence="13">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">nrd</name>
        <name evidence="15" type="ordered locus">SSO0929</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ribonucleoside diphosphate reductase class-2 family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="13"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90244">
        <property type="entry name" value="B90244"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZH9"/>
    <dbReference type="SMR" id="Q97ZH9"/>
    <dbReference type="FunCoup" id="Q97ZH9">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0929"/>
    <dbReference type="PaxDb" id="273057-SSO0929"/>
    <dbReference type="EnsemblBacteria" id="AAK41209">
        <property type="protein sequence ID" value="AAK41209"/>
        <property type="gene ID" value="SSO0929"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0929"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.929"/>
    <dbReference type="eggNOG" id="arCOG04276">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000404_2_0_2"/>
    <dbReference type="InParanoid" id="Q97ZH9"/>
    <dbReference type="PhylomeDB" id="Q97ZH9"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02888">
        <property type="entry name" value="RNR_II_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050862">
        <property type="entry name" value="RdRp_reductase_class-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013344">
        <property type="entry name" value="RNR_NrdJ/NrdZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02504">
        <property type="entry name" value="NrdJ_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43371:SF1">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43371">
        <property type="entry name" value="VITAMIN B12-DEPENDENT RIBONUCLEOTIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="10 13" id="KW-0170">Cobalt</keyword>
    <keyword evidence="8" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="5 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 13 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="14">
        <location>
            <begin position="16"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAK41209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="841" mass="95214" checksum="C18DA1D94A028D69" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZJ4</accession>
    <name>Q97ZJ4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO6778</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90242">
        <property type="entry name" value="C90242"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992341.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZJ4"/>
    <dbReference type="STRING" id="273057.SSO6778"/>
    <dbReference type="PaxDb" id="273057-SSO6778"/>
    <dbReference type="EnsemblBacteria" id="AAK41194">
        <property type="protein sequence ID" value="AAK41194"/>
        <property type="gene ID" value="SSO6778"/>
    </dbReference>
    <dbReference type="GeneID" id="1455161"/>
    <dbReference type="KEGG" id="sso:SSO6778"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.914"/>
    <dbReference type="eggNOG" id="arCOG08458">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191161_1_0_2"/>
    <dbReference type="InParanoid" id="Q97ZJ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="83" mass="8942" checksum="073C9CEFB3A2CE04" modified="2001-10-01" version="1">MSIQALSNVSSQFSHLLSNINIEPISYILVIIGFALLLIIIIGSVIYGLTKAARAVPSMSTKEFILFLLGIAIFLVILGILLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZR9</accession>
    <name>Q97ZR9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90196">
        <property type="entry name" value="G90196"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZR9"/>
    <dbReference type="STRING" id="273057.SSO0510"/>
    <dbReference type="PaxDb" id="273057-SSO0510"/>
    <dbReference type="EnsemblBacteria" id="AAK40830">
        <property type="protein sequence ID" value="AAK40830"/>
        <property type="gene ID" value="SSO0510"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0510"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2010196_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZR9"/>
    <dbReference type="PhylomeDB" id="Q97ZR9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="123" mass="13895" checksum="0FF00E64D7C0D9BE" modified="2001-10-01" version="1">MVDPYPLASSPQGEYLVEYLGTPIKLLVIDDYKGYGRRYFFSTDLNDTAEDIITTWENRWDIEVLIRELKALGLEGGSFLTWVRNSGFVALKALSPRCPILQVLHGSGTRGQKVGQIDKKYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZT8</accession>
    <name>Q97ZT8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphate-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pstS</name>
        <name evidence="7" type="ordered locus">SSO0489</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the PstS family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90194">
        <property type="entry name" value="C90194"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923004.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZT8"/>
    <dbReference type="FunCoup" id="Q97ZT8">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0489"/>
    <dbReference type="PaxDb" id="273057-SSO0489"/>
    <dbReference type="EnsemblBacteria" id="AAK40810">
        <property type="protein sequence ID" value="AAK40810"/>
        <property type="gene ID" value="SSO0489"/>
    </dbReference>
    <dbReference type="GeneID" id="1455620"/>
    <dbReference type="GeneID" id="27426786"/>
    <dbReference type="KEGG" id="sso:SSO0489"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.483"/>
    <dbReference type="eggNOG" id="arCOG00213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034528_1_2_2"/>
    <dbReference type="InParanoid" id="Q97ZT8"/>
    <dbReference type="PhylomeDB" id="Q97ZT8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042301">
        <property type="term" value="F:phosphate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13565">
        <property type="entry name" value="PBP2_PstS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005673">
        <property type="entry name" value="ABC_phos-bd_PstS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050962">
        <property type="entry name" value="Phosphate-bind_PstS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00975">
        <property type="entry name" value="3a0107s03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42996">
        <property type="entry name" value="PHOSPHATE-BINDING PROTEIN PSTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42996:SF1">
        <property type="entry name" value="PHOSPHATE-BINDING PROTEIN PSTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002756">
        <property type="entry name" value="PstS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 4" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="PBP" evidence="6">
        <location>
            <begin position="55"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="405" mass="43697" checksum="179D6D037CA265D9" modified="2001-10-01" version="1">MKGFSTLAVVIILIIVVIAVAGIFFVINSQGGHNTTTTSTSSSFSTSSQASSSQTTASSQISASIIAGGSTFVNPLMQVWIKDFQSNYNGIQVTYSAVGSGAGVNNFLQGAYDIGATDVPPPSNLYQQLTQKYGEVLTIPDVVGAVDIIYNIPSFSGTLNLTADVLAKIYLGQIQYWDDPAIKALNPHFNFTHQKIIAVHRSDGSGTTFIFTYWLYTSSQSWRSSNVSYGYTVNWPVDKLGNGLGGKGSDGVTAYVSQNPYSIGYVEAQYAIAKNLTPAAVLNPSTGDYVLPTQASIETAIQNANLSSLPSSLTRDLSQYLSVFLNVKAHNAYPIVTFSWLVIKVNYTDKSKAEAIYLFLKYIATTGQTELPSGYIELPSNIQQLILQNLKLISYNNTSVYTLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZU8</accession>
    <name>Q97ZU8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90193">
        <property type="entry name" value="A90193"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922994.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZU8"/>
    <dbReference type="STRING" id="273057.SSO0479"/>
    <dbReference type="PaxDb" id="273057-SSO0479"/>
    <dbReference type="EnsemblBacteria" id="AAK40800">
        <property type="protein sequence ID" value="AAK40800"/>
        <property type="gene ID" value="SSO0479"/>
    </dbReference>
    <dbReference type="GeneID" id="84059034"/>
    <dbReference type="KEGG" id="sso:SSO0479"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.473"/>
    <dbReference type="eggNOG" id="arCOG04583">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107501_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZU8"/>
    <dbReference type="PhylomeDB" id="Q97ZU8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="19"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="182" mass="21277" checksum="E97F7EB93D725BCF" modified="2001-10-01" version="1">MYRRKPNPYVKLINGDKIIDVGCGSGQNCDQFKGRLVICLDLSLNQLNQARKKGCQNLVQADMEYLPFRDSSVDSLAYIASLHHLRDPSQALGEAQRVLKDKGEILVTVWLVQLKFFFLRRYIIKRSLINGKEVRRFYRLYYPWELKRIMESKGFVTKIYKLYRVNSLLPNNSLYYGIKSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZV1</accession>
    <name>Q97ZV1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF790 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90192">
        <property type="entry name" value="F90192"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922991.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZV1"/>
    <dbReference type="STRING" id="273057.SSO0475"/>
    <dbReference type="PaxDb" id="273057-SSO0475"/>
    <dbReference type="EnsemblBacteria" id="AAK40797">
        <property type="protein sequence ID" value="AAK40797"/>
        <property type="gene ID" value="SSO0475"/>
    </dbReference>
    <dbReference type="GeneID" id="7807121"/>
    <dbReference type="KEGG" id="sso:SSO0475"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.470"/>
    <dbReference type="eggNOG" id="arCOG04356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038336_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZV1"/>
    <dbReference type="PhylomeDB" id="Q97ZV1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.91.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008508">
        <property type="entry name" value="Bax1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39640:SF1">
        <property type="entry name" value="DUF790 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39640">
        <property type="entry name" value="VNG6129C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05626">
        <property type="entry name" value="DUF790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019435">
        <property type="entry name" value="UCP019435"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="57061" checksum="CB8579387F183CAF" modified="2001-10-01" version="1">MLTSDLARFKIENQRIIPLFATDSDIDVAKEVIDMFKIGAKVGDILEDLKYLSKIYDYKLVKGLGKIYLRYCIVESATKIDYIELRRQLFSRGPVLEENDKERVLKEVGDLFHVDPIKAMYEDLDVEKKIVELPKFSPEDLLKIYNLSLLQTIIFNAYKVTVSVSDGWKEIARRIKMLGLMYLAYENPLRIEIFGPLSLVKMTEKYGRNLAALVPFLVSKNKWTIIADIVLGKNKRRTYRLELSSGYSKLFKYINDEEMEKRFDSSIEEKFYEEFRRVIRDWNIVREPEPIVIEKRLYFPDFVLSKGNIKVYVEIMGFWTKEYVNSKIEKLRNFKYPILVLLNEELSYENYIPDTLNVIKFKKKIDIGKLYSALRGFQANVNEDIDLSDVNDDIILIKELSAKYNVNEKIVRSKLMQRPDYIVLKNYAIKKTFIEELKKEDFSNTQLSSLVNKYGNYIVDIIDYLGYKIVWKNISDAIVEKIKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZY4</accession>
    <name>Q97ZY4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90188">
        <property type="entry name" value="D90188"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988733.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZY4"/>
    <dbReference type="STRING" id="273057.SSO0438"/>
    <dbReference type="PaxDb" id="273057-SSO0438"/>
    <dbReference type="EnsemblBacteria" id="AAK40763">
        <property type="protein sequence ID" value="AAK40763"/>
        <property type="gene ID" value="SSO0438"/>
    </dbReference>
    <dbReference type="GeneID" id="1455573"/>
    <dbReference type="KEGG" id="sso:SSO0438"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.431"/>
    <dbReference type="eggNOG" id="arCOG05464">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138325_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZY4"/>
    <dbReference type="PhylomeDB" id="Q97ZY4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="129" mass="15036" checksum="D1FFBF263622DB69" modified="2001-10-01" version="1">MRNGNRGFERQPSFTDEQKPMPLGDKCNYLCPYFRCNKRALLVQVKYAKGNPYKVGYCRWVGDVCITGECQYAYCEKRALLPGNKCAFAINKKNERDNEIEKELEKEDYDDKMKEIISKKFGKKGLDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZZ4</accession>
    <name>Q97ZZ4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribbon-helix-helix protein CopG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO5826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90186">
        <property type="entry name" value="H90186"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988754.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZZ4"/>
    <dbReference type="STRING" id="273057.SSO5826"/>
    <dbReference type="PaxDb" id="273057-SSO5826"/>
    <dbReference type="EnsemblBacteria" id="AAK40751">
        <property type="protein sequence ID" value="AAK40751"/>
        <property type="gene ID" value="SSO5826"/>
    </dbReference>
    <dbReference type="GeneID" id="42361872"/>
    <dbReference type="KEGG" id="sso:SSO5826"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.418"/>
    <dbReference type="eggNOG" id="arCOG01009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_202919_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZZ4"/>
    <dbReference type="PhylomeDB" id="Q97ZZ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36215">
        <property type="entry name" value="BLL4998 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36215:SF1">
        <property type="entry name" value="BLL4998 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="1">
        <location>
            <begin position="2"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="50" mass="5781" checksum="C860AC855BB5F864" modified="2001-10-01" version="1">MKIITVKLPEQFLEAIDELVNTGRYGSRSEVIRAAIGDFIRKELWITTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q980D7</accession>
    <name>Q980D7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cobalamin adenosyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO0377</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90181">
        <property type="entry name" value="C90181"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988825.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980D7"/>
    <dbReference type="FunCoup" id="Q980D7">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0377"/>
    <dbReference type="PaxDb" id="273057-SSO0377"/>
    <dbReference type="EnsemblBacteria" id="AAK40706">
        <property type="protein sequence ID" value="AAK40706"/>
        <property type="gene ID" value="SSO0377"/>
    </dbReference>
    <dbReference type="GeneID" id="1455516"/>
    <dbReference type="KEGG" id="sso:SSO0377"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.370"/>
    <dbReference type="eggNOG" id="arCOG00489">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083486_0_2_2"/>
    <dbReference type="InParanoid" id="Q980D7"/>
    <dbReference type="PhylomeDB" id="Q980D7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:corrinoid adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1200.10">
        <property type="entry name" value="Cobalamin adenosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016030">
        <property type="entry name" value="CblAdoTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036451">
        <property type="entry name" value="CblAdoTrfase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029499">
        <property type="entry name" value="PduO-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00636">
        <property type="entry name" value="PduO_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213:SF0">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE MMAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01923">
        <property type="entry name" value="Cob_adeno_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89028">
        <property type="entry name" value="Cobalamin adenosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cobalamin adenosyltransferase-like" evidence="4">
        <location>
            <begin position="5"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="172" mass="19628" checksum="E830DC9507DC104F" modified="2001-10-01" version="1">MTVWYTGTGDKGKTKLPSVGEIWKDDDIVNAIGDLDELNASLGVISSLYPQLREIMVRIQSDIFSLSSEIAGFDMNFEEEKVKWIEEEISSFSKGIPELRNFILPGGHVAASFLHLARTICRRSERSVVKILKRSKAKDVHVKYLNRLSSLLFVLALYVNKQEKVQEIIWKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q980J7</accession>
    <name>RL10E_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L10e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl10e</name>
        <name type="ordered locus">SSO0294</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90172">
        <property type="entry name" value="C90172"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990585.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980J7"/>
    <dbReference type="SMR" id="Q980J7"/>
    <dbReference type="FunCoup" id="Q980J7">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0294"/>
    <dbReference type="PaxDb" id="273057-SSO0294"/>
    <dbReference type="EnsemblBacteria" id="AAK40634">
        <property type="protein sequence ID" value="AAK40634"/>
        <property type="gene ID" value="SSO0294"/>
    </dbReference>
    <dbReference type="GeneID" id="1455444"/>
    <dbReference type="KEGG" id="sso:SSO0294"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.289"/>
    <dbReference type="eggNOG" id="arCOG04113">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084051_0_2_2"/>
    <dbReference type="InParanoid" id="Q980J7"/>
    <dbReference type="PhylomeDB" id="Q980J7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.10:FF:000008">
        <property type="entry name" value="50S ribosomal protein L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00448">
        <property type="entry name" value="Ribosomal_uL16_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022981">
        <property type="entry name" value="Ribosomal_uL16_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018255">
        <property type="entry name" value="Ribosomal_uL16_CS_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_uL16_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003236">
        <property type="entry name" value="PRK04199.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003239">
        <property type="entry name" value="PRK04199.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01257">
        <property type="entry name" value="RIBOSOMAL_L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000147148" description="Large ribosomal subunit protein uL16">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="178" mass="20363" checksum="3545A88FDBA26F18" modified="2001-09-26" version="1">MPLRPGRCYRHFSGPAYTRKEYIPGIPQPKITKFTSGNPNGDYDYEVRLITTEIGQIRHNALEAVRTLTLKTLSKKTGSETSFFMWILKYPHHVLRENKMMAFAGADRLQDGMRLSFGTPIGTAARIEKLGETLIVIKVKKEHLEFAKEALKIASKKLPLRTRIEIIPLRLVRQEVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="149" xmlns="https://uniprot.org/uniprot">
    <accession>Q980K5</accession>
    <name>Q980K5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNase L inhibitor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO0287</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2010-09" db="PDB data bank">
            <title>Ribosome Recycling in Archaea A Mechanistic Link between FeS Cluster Biosynthesis and Conformational Switch of Twin-ATPase ABCE1.</title>
            <authorList>
                <person name="Barthelme D."/>
                <person name="Dinkelaker S."/>
                <person name="Albers S.-V."/>
                <person name="Londei P."/>
                <person name="Ermler U."/>
                <person name="Tampe R."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 76-600 IN COMPLEX WITH ADP AND MG(2+)</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="journal article" date="2020" name="EMBO J." volume="39" first="e103788" last="e103788">
            <title>Molecular analysis of the ribosome recycling factor ABCE1 bound to the 30S post-splitting complex.</title>
            <authorList>
                <person name="Nurenberg-Goloub E."/>
                <person name="Kratzat H."/>
                <person name="Heinemann H."/>
                <person name="Heuer A."/>
                <person name="Kotter P."/>
                <person name="Berninghausen O."/>
                <person name="Becker T."/>
                <person name="Tampe R."/>
                <person name="Beckmann R."/>
            </authorList>
            <dbReference type="PubMed" id="32064661"/>
            <dbReference type="DOI" id="10.15252/embj.2019103788"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.80 ANGSTROMS) OF 1-594 IN COMPLEX WITH MG(2+) AND [4FE-4S] CLUSTER</scope>
    </reference>
    <comment type="interaction">
        <interactant intactId="EBI-15910144">
            <id>Q980K5</id>
        </interactant>
        <interactant intactId="EBI-15910165">
            <id>Q97W96</id>
            <label>prf1</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>2</experiments>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90171">
        <property type="entry name" value="C90171"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990572.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3OZX">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.05 A"/>
        <property type="chains" value="A/B=76-600"/>
    </dbReference>
    <dbReference type="PDB" id="6TMF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="d=1-594"/>
    </dbReference>
    <dbReference type="PDBsum" id="3OZX"/>
    <dbReference type="AlphaFoldDB" id="Q980K5"/>
    <dbReference type="SMR" id="Q980K5"/>
    <dbReference type="DIP" id="DIP-60310N"/>
    <dbReference type="FunCoup" id="Q980K5">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="IntAct" id="Q980K5">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0287"/>
    <dbReference type="PaxDb" id="273057-SSO0287"/>
    <dbReference type="EnsemblBacteria" id="AAK40626">
        <property type="protein sequence ID" value="AAK40626"/>
        <property type="gene ID" value="SSO0287"/>
    </dbReference>
    <dbReference type="GeneID" id="1455436"/>
    <dbReference type="KEGG" id="sso:SSO0287"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.282"/>
    <dbReference type="eggNOG" id="arCOG00187">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017344_4_1_2"/>
    <dbReference type="InParanoid" id="Q980K5"/>
    <dbReference type="PhylomeDB" id="Q980K5"/>
    <dbReference type="EvolutionaryTrace" id="Q980K5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004090">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
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        <property type="match status" value="2"/>
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        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="PRK13409.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="7 8" id="KW-0002">3D-structure</keyword>
    <keyword evidence="8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="42"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="69"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="331"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="83"/>
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            <name>Mg(2+)</name>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51379"/>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
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    </evidence>
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        <source>
            <dbReference type="PDB" id="3OZX"/>
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    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="6TMF"/>
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    </evidence>
    <sequence length="600" mass="67882" checksum="764949028BEF8539" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q980P2</accession>
    <name>Q980P2_SACS2</name>
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        <submittedName>
            <fullName evidence="8">Probable chorismate mutase (CM)/prephenate dehydratase (PDT) (PheA)</fullName>
            <ecNumber evidence="8">4.2.1.51</ecNumber>
            <ecNumber evidence="8">5.4.99.5</ecNumber>
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    </protein>
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        <name evidence="8" type="primary">pheA</name>
        <name evidence="8" type="ordered locus">SSO0246</name>
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    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.51" evidence="8"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="PaxDb" id="273057-SSO0246"/>
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        <property type="gene ID" value="SSO0246"/>
    </dbReference>
    <dbReference type="GeneID" id="1455396"/>
    <dbReference type="KEGG" id="sso:SSO0246"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.241"/>
    <dbReference type="eggNOG" id="arCOG00255">
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
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        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
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    </dbReference>
    <dbReference type="CDD" id="cd04905">
        <property type="entry name" value="ACT_CM-PDT"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PBP2_PDT_1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022:SF19">
        <property type="entry name" value="PREPHENATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACT"/>
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    </dbReference>
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        <property type="entry name" value="PDT"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Periplasmic binding protein-like II"/>
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    </dbReference>
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        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate dehydratase" evidence="6">
        <location>
            <begin position="6"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="187"/>
            <end position="268"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
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            <dbReference type="ARBA" id="ARBA00023141"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAK40586.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="269" mass="30536" checksum="8512666CB36588B7" modified="2001-10-01" version="1">MVDADGIYYLGPEGSFTHEAASLFKGKDLKAESTISLVFKRVYERESSIGIVPIENSLEGPVNETLDNLYNYDNIYVIGEIEKKIELVLAARSDNLSQIKRIYSHPHAFNEAREKLKELGFNEYIPVESTSKAAKIVSQDSEAAAICSNFAAKLYNLKVLITISSHNNYTRFIVLSKEMNSNGNKSMILFTVPHKPGALYKVLQVFYEYNINISMIYSRPLKSIPWQYYFYLEYEGGISNREFIEKLVKSTSVLKIKGSFSKLNNDPGF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-11-16" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q980R2</accession>
    <name>RPO1N_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerase subunit Rpo1N</fullName>
            <ecNumber evidence="2">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">DNA-directed RNA polymerase subunit A'</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpo1N</name>
        <name evidence="2" type="synonym">rpoA1</name>
        <name type="ordered locus">SSO0225</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="13595" last="13595">
            <title>Repression of RNA polymerase by the archaeo-viral regulator ORF145/RIP.</title>
            <authorList>
                <person name="Sheppard C."/>
                <person name="Blombach F."/>
                <person name="Belsom A."/>
                <person name="Schulz S."/>
                <person name="Daviter T."/>
                <person name="Smollett K."/>
                <person name="Mahieu E."/>
                <person name="Erdmann S."/>
                <person name="Tinnefeld P."/>
                <person name="Garrett R."/>
                <person name="Grohmann D."/>
                <person name="Rappsilber J."/>
                <person name="Werner F."/>
            </authorList>
            <dbReference type="PubMed" id="27882920"/>
            <dbReference type="DOI" id="10.1038/ncomms13595"/>
        </citation>
        <scope>INTERACTION WITH ACIDIANUS TWO-TAILED VIRUS PROTEIN RIP (MICROBIAL INFECTION)</scope>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2008" name="Nature" volume="451" first="851" last="854">
            <title>The X-ray crystal structure of RNA polymerase from Archaea.</title>
            <authorList>
                <person name="Hirata A."/>
                <person name="Klein B.J."/>
                <person name="Murakami K.S."/>
            </authorList>
            <dbReference type="PubMed" id="18235446"/>
            <dbReference type="DOI" id="10.1038/nature06530"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF THE RNA POLYMERASE COMPLEX IN COMPLEX WITH MAGNESIUM AND ZINC</scope>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms the clamp head domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3 5 6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3 5 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1 2 3 5 6">Binds 3 Zn(2+) per subunit.</text>
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    <comment type="subunit">
        <text evidence="4">(Microbial infection) Interacts with acidianus two-tailed virus protein RIP; this interaction results in the global inhibition of transcription.</text>
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    <comment type="subunit">
        <text evidence="3">Part of the 13-subunit RNA polymerase complex.</text>
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        <source>
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        </source>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_00863"/>
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        <source>
            <dbReference type="PubMed" id="18235446"/>
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        <source>
            <dbReference type="PDB" id="3HKZ"/>
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        <source>
            <dbReference type="PDB" id="2PMZ"/>
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            <dbReference type="PDB" id="3HKZ"/>
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    <sequence length="880" mass="99675" checksum="5C8AE20EFC71DE25" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q980T3</accession>
    <name>Q980T3_SACS2</name>
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        <recommendedName>
            <fullName evidence="2">Mut7-C RNAse domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0194</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="E90160"/>
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    <dbReference type="RefSeq" id="WP_009990432.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    <dbReference type="PANTHER" id="PTHR39081:SF1">
        <property type="entry name" value="MUT7-C RNASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mut7-C"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="7"/>
            <end position="138"/>
        </location>
    </feature>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01927"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAK40540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="176" mass="20536" checksum="B53DD81675447266" modified="2001-10-01" version="1">MRTMLTQKFVVDSMLGKIARWLRIMGYDTLYSNDFEDWKILKIAETQKRIIITRDRGIYYRSLKRGLNCILLSPDSNIIQDLAFIALRSKIDLSVNINATRCPQCNSVLSKLSESMWLCPKCKKEYWKGRHWRTIEEIIIKANSELVKLETKNDSRGTGTDTGVKRRDRTVTNRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q980U6</accession>
    <name>Q980U6_SACS2</name>
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            <fullName evidence="4 12">Delta-aminolevulinic acid dehydratase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">SSO0181</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>2 5-aminolevulinate = porphobilinogen + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ALAD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="3 12"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="H90158"/>
    </dbReference>
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        <property type="interactions" value="312"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0181"/>
    <dbReference type="PaxDb" id="273057-SSO0181"/>
    <dbReference type="EnsemblBacteria" id="AAK40527">
        <property type="protein sequence ID" value="AAK40527"/>
        <property type="gene ID" value="SSO0181"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0181"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.179"/>
    <dbReference type="eggNOG" id="arCOG04300">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035731_0_0_2"/>
    <dbReference type="InParanoid" id="Q980U6"/>
    <dbReference type="PhylomeDB" id="Q980U6"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00384">
        <property type="entry name" value="ALAD_PBGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000019">
        <property type="entry name" value="Delta-aminolevulinic acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006762">
        <property type="entry name" value="PRK09283.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458:SF0">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAK40527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="341" mass="38263" checksum="D4CA6DD071ECEF07" modified="2001-10-01" version="1">MIFLKMVTFPVLRPRRLRKSKLVRDLVSETSISQNDLVLPVFIKENINEPEQIASMPGIYRYPPNDKLINYLQDSYENGIRSVILFGIPSYKDDIASSAYDKNGIIQRAVRIIKDSFKDKIIVITDECTDEYMANGHCGLVKYTNGCYVVDNDESLKIHAKIALSQAEAGADVIAPSSMMDGVVRAIRDALDSAGYIDTLILSYSVKYASVMYGPFRDAAYSKPAFGDRRGYQMDPRNAFEAIKEARLDIEEGADILMVKPAHTYLDVIRLVKDHFPEYPLAAYHVSGEYSMIKAAAINGWIDEKTAVLEITTAIKRAGADLIITYYANDIARWIKDGVPF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q980X0</accession>
    <name>LYSZ_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">[LysW]-aminoadipate/[LysW]-glutamate kinase</fullName>
            <ecNumber evidence="1">2.7.2.17</ecNumber>
            <ecNumber evidence="1">2.7.2.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">lysZ</name>
        <name evidence="2" type="synonym">argB</name>
        <name type="ordered locus">SSO0156</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="29537" last="29549">
            <title>The Sulfolobus solfataricus Lrp-like protein LysM regulates lysine biosynthesis in response to lysine availability.</title>
            <authorList>
                <person name="Brinkman A.B."/>
                <person name="Bell S.D."/>
                <person name="Lebbink R.J."/>
                <person name="de Vos W.M."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="12042311"/>
            <dbReference type="DOI" id="10.1074/jbc.M203528200"/>
        </citation>
        <scope>INVOLVEMENT IN LYSINE BIOSYNTHESIS</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in both the arginine and lysine biosynthetic pathways. Phosphorylates the LysW-bound precursors glutamate (for arginine biosynthesis), respectively alpha-aminoadipate (for lysine biosynthesis).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[amino-group carrier protein]-C-terminal-N-(1,4-dicarboxybutan-1-yl)-L-glutamine + ATP = [amino-group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine + ADP</text>
            <dbReference type="Rhea" id="RHEA:41944"/>
            <dbReference type="Rhea" id="RHEA-COMP:9694"/>
            <dbReference type="Rhea" id="RHEA-COMP:9712"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:78499"/>
            <dbReference type="ChEBI" id="CHEBI:78503"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-glutamyl)-L-glutamate + ATP = [amino-group carrier protein]-C-terminal-gamma-(5-phospho-L-glutamyl)-L-glutamate + ADP</text>
            <dbReference type="Rhea" id="RHEA:52632"/>
            <dbReference type="Rhea" id="RHEA-COMP:13311"/>
            <dbReference type="Rhea" id="RHEA-COMP:13313"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:136714"/>
            <dbReference type="ChEBI" id="CHEBI:136717"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 2/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetylglutamate kinase family. LysZ subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.17" evidence="1"/>
    <dbReference type="EC" id="2.7.2.19" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BK000545">
        <property type="protein sequence ID" value="DAA00050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90155">
        <property type="entry name" value="G90155"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990381.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980X0"/>
    <dbReference type="SMR" id="Q980X0"/>
    <dbReference type="FunCoup" id="Q980X0">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0156"/>
    <dbReference type="PaxDb" id="273057-SSO0156"/>
    <dbReference type="EnsemblBacteria" id="AAK40502">
        <property type="protein sequence ID" value="AAK40502"/>
        <property type="gene ID" value="SSO0156"/>
    </dbReference>
    <dbReference type="GeneID" id="1453329"/>
    <dbReference type="KEGG" id="sso:SSO0156"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.153"/>
    <dbReference type="eggNOG" id="arCOG00862">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053680_2_0_2"/>
    <dbReference type="InParanoid" id="Q980X0"/>
    <dbReference type="PhylomeDB" id="Q980X0"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00036"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043744">
        <property type="term" value="F:N2-acetyl-L-aminoadipate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04251">
        <property type="entry name" value="AAK_NAGK-UC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02082">
        <property type="entry name" value="LysZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037529">
        <property type="entry name" value="LysZ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010662">
        <property type="entry name" value="PRK14058.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342:SF0">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000112704" description="[LysW]-aminoadipate/[LysW]-glutamate kinase">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="35"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="5"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="12042311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3">
        <source>
            <dbReference type="PubMed" id="12042311"/>
        </source>
    </evidence>
    <sequence length="264" mass="28552" checksum="D15458B8FEA0AC48" modified="2001-10-01" version="1">MIVVKIGGRVVKNSLDKIILDILNINDKVILVHGGGDIVTDYTKRLGIEPVFVTSPEGIRSRYTTKEELEVYIMAMSLINKSITSKLCSLGKNAIGITGVDGGLLLAERKKRIVVIDERGKKRIIEGGYTGKVKEVRSEIINHLVKLFDIIVVSPIALDVEERTPLNIDGDQAAFAISKALRANVLILLSDVEGVLVEGKVINRLTPSEAKELSKKIGPGMNRKLLMAAESVENGVNKVIIGSGVKDRPIINALELNGTVIANG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q980X3</accession>
    <name>Q980X3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0152</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90155">
        <property type="entry name" value="D90155"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990378.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980X3"/>
    <dbReference type="STRING" id="273057.SSO0152"/>
    <dbReference type="PaxDb" id="273057-SSO0152"/>
    <dbReference type="EnsemblBacteria" id="AAK40499">
        <property type="protein sequence ID" value="AAK40499"/>
        <property type="gene ID" value="SSO0152"/>
    </dbReference>
    <dbReference type="GeneID" id="1453326"/>
    <dbReference type="KEGG" id="sso:SSO0152"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.150"/>
    <dbReference type="eggNOG" id="arCOG05935">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_516412_0_0_2"/>
    <dbReference type="InParanoid" id="Q980X3"/>
    <dbReference type="PhylomeDB" id="Q980X3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01127">
        <property type="entry name" value="TrwB_TraG_TraD_VirD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053657">
        <property type="entry name" value="Ced-DNA_import"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051162">
        <property type="entry name" value="T4SS_component"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041017">
        <property type="entry name" value="DNA_import_CedB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121:SF6">
        <property type="entry name" value="SLR6007 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJGR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="278"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="600" mass="69201" checksum="6D87FDDD4E66DA29" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Y8</accession>
    <name>Q980Y8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase A24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO0131</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90153">
        <property type="entry name" value="E90153"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Y8"/>
    <dbReference type="STRING" id="273057.SSO0131"/>
    <dbReference type="MEROPS" id="A24.017"/>
    <dbReference type="PaxDb" id="273057-SSO0131"/>
    <dbReference type="EnsemblBacteria" id="AAK40484">
        <property type="protein sequence ID" value="AAK40484"/>
        <property type="gene ID" value="SSO0131"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0131"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.126"/>
    <dbReference type="eggNOG" id="arCOG02298">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102058_0_0_2"/>
    <dbReference type="InParanoid" id="Q980Y8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052218">
        <property type="entry name" value="Preflagellin_Peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009655">
        <property type="entry name" value="Preflagellin_peptidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000045">
        <property type="entry name" value="Prepilin_IV_endopep_pep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36506">
        <property type="entry name" value="PREFLAGELLIN PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36506:SF1">
        <property type="entry name" value="PREFLAGELLIN PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06847">
        <property type="entry name" value="Arc_PepC_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01478">
        <property type="entry name" value="Peptidase_A24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Prepilin type IV endopeptidase peptidase" evidence="7">
        <location>
            <begin position="11"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Preflagellin peptidase C-terminal" evidence="8">
        <location>
            <begin position="142"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK40484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="27498" checksum="762922E55F2E12F6" modified="2001-10-01" version="1">MIVIYYIQIFLSIIMLIHTSILDLKYREVDPKIWIYYSPLCLFIIFSFHNLFLPIYLYSFIITNLLFLILYKLSLLGGADLFLNVILSLANASVISLVPSRFLVIGLEPLVIVLYSSIIILIFSLINFVKQYKYVRNLPFSKKILLALSAKRIKIRDFINSKFLFPLTEVKEDGSIMLRDYFSVEEDDKYWRELYSKLVNEGKISADTYIWVAWGIPVIPFMLAGYIMSLIIGFPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Z4</accession>
    <name>Q980Z4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chorismate dehydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90152">
        <property type="entry name" value="G90152"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988948.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Z4"/>
    <dbReference type="STRING" id="273057.SSO0123"/>
    <dbReference type="PaxDb" id="273057-SSO0123"/>
    <dbReference type="EnsemblBacteria" id="AAK40478">
        <property type="protein sequence ID" value="AAK40478"/>
        <property type="gene ID" value="SSO0123"/>
    </dbReference>
    <dbReference type="GeneID" id="1453305"/>
    <dbReference type="KEGG" id="sso:SSO0123"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.119"/>
    <dbReference type="eggNOG" id="arCOG05985">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1253637_0_0_2"/>
    <dbReference type="InParanoid" id="Q980Z4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="25303" checksum="9408DFDE11F6BB0D" modified="2001-10-01" version="1">MRLSLGYVKEKELEIIAPLLNNETNRNIEFNLIRVKEDELKFKLKELDLAFIPLPLTLFYENIRFISNGAFVANKIGLKIINSRFSKLCINSSNSTEYYLFKTLTNYKNPLEIVRSEVCNDADVLISYEDYDISLDELWRKNCGDLPLVISLIGSLRLDEQTLSKVKVVIREAASLQESRGRISHYSKELGLKGRQAIECFFQLCLKRGLCRSSISPQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q981B7</accession>
    <name>Q981B7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO0029</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90142">
        <property type="entry name" value="D90142"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989611.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981B7"/>
    <dbReference type="FunCoup" id="Q981B7">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0029"/>
    <dbReference type="PaxDb" id="273057-SSO0029"/>
    <dbReference type="EnsemblBacteria" id="AAK40395">
        <property type="protein sequence ID" value="AAK40395"/>
        <property type="gene ID" value="SSO0029"/>
    </dbReference>
    <dbReference type="GeneID" id="1455286"/>
    <dbReference type="KEGG" id="sso:SSO0029"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.31"/>
    <dbReference type="eggNOG" id="arCOG00601">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_734888_0_0_2"/>
    <dbReference type="InParanoid" id="Q981B7"/>
    <dbReference type="PhylomeDB" id="Q981B7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.100">
        <property type="entry name" value="Ribosome hibernation promotion factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036567">
        <property type="entry name" value="RHF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014651">
        <property type="entry name" value="UCP036983_2CBS_MJ1404"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036983">
        <property type="entry name" value="UCP_2CBS_MJ1404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69754">
        <property type="entry name" value="Ribosome binding protein Y (YfiA homologue)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="6"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="66"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="132"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="208"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="346"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="380" mass="43434" checksum="7A79D143809B5D2C" modified="2001-10-01" version="1">MIDNTLITKPSYVAHSMDKLSDIISKMKENKMWTVPVIKDRKLIGLISYKDLLSRRVSLETKAINIMSPSVTVQIDEDINRLIAKFYTTKARVIPVINEKREFIGLVTRESLLSYLLKANEIPENRTAREYMTSPATSIDENDSIARARWLMIRDNISRLPATKEYRLTGIISARDIVDALYSVSGRKRESIMKDEERVMAMPVKEIMKYPVITANGKDSLTDVVEKLLKFRISGMPVMEGDRLSGVISGLDIIKAVAEKMQLSIPIEAKIPQELKSNLEFKANIDDILERYLSKIERLTEVINFKVSFKEEMRSSTGNKKLYTAMVRVTTKIGDYVARDTDWEPVVALKNAVEKIEERILRKLRKIEESNKKGIKAEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q981D0</accession>
    <name>Q981D0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO0015</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90140">
        <property type="entry name" value="G90140"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989633.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981D0"/>
    <dbReference type="STRING" id="273057.SSO0015"/>
    <dbReference type="PaxDb" id="273057-SSO0015"/>
    <dbReference type="EnsemblBacteria" id="AAK40382">
        <property type="protein sequence ID" value="AAK40382"/>
        <property type="gene ID" value="SSO0015"/>
    </dbReference>
    <dbReference type="GeneID" id="1455273"/>
    <dbReference type="KEGG" id="sso:SSO0015"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.16"/>
    <dbReference type="eggNOG" id="arCOG02050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_5_2_2"/>
    <dbReference type="InParanoid" id="Q981D0"/>
    <dbReference type="PhylomeDB" id="Q981D0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052017">
        <property type="entry name" value="TSUP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30269:SF37">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30269">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN YFCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK40382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="286" mass="30896" checksum="4D35ED20AC203AF6" modified="2001-10-01" version="1">MLTPIEFFVTVVLISALSGILGALTGLGGATFLVPIYTLYLNVPIAYASGASLISTIATSSGAASAYVKDRITNVKIGMGLEIATTTGSIAGALTVAYIYAHHLEFIVYIVFGIVLLSQVYVQLAKSKFELPKPIKPDWTTRVFELYGKYYDTVLKQEVEYHGVRWWLGEIIMFFAGFISGLLGIGSGALKVLGMDWAMNLPMKVSTTTSNFMIGVTAATGSSIYWIFGYIQPFLAAGTALGVLIGAFVGTKILVRVTNKTIRYIFTAILIFLGIQMILRGFGYGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX92</accession>
    <name>RS8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein uS8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">30S ribosomal protein S8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps8</name>
        <name evidence="5" type="synonym">rps8AB</name>
        <name type="ordered locus">SSO0703</name>
        <name type="ORF">C10_027</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7 8 9" key="3">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90218">
        <property type="entry name" value="E90218"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991261.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="I=1-133"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="I=1-133"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="I=1-133"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="I=1-133"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="I=1-133"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="I=1-133"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="I=1-133"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="I=1-133"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q9UX92"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q9UX92"/>
    <dbReference type="FunCoup" id="Q9UX92">
        <property type="interactions" value="209"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0703"/>
    <dbReference type="PaxDb" id="273057-SSO0703"/>
    <dbReference type="EnsemblBacteria" id="AAK41004">
        <property type="protein sequence ID" value="AAK41004"/>
        <property type="gene ID" value="SSO0703"/>
    </dbReference>
    <dbReference type="GeneID" id="1454971"/>
    <dbReference type="KEGG" id="sso:SSO0703"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.703"/>
    <dbReference type="eggNOG" id="arCOG04091">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098428_1_1_2"/>
    <dbReference type="InParanoid" id="Q9UX92"/>
    <dbReference type="PhylomeDB" id="Q9UX92"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
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        <property type="entry name" value="Ribosomal_S8"/>
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        <property type="entry name" value="Ribosomal protein S8"/>
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        <property type="entry name" value="RIBOSOMAL_S8"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
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    <keyword id="KW-0689">Ribosomal protein</keyword>
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        <location>
            <begin position="1"/>
            <end position="133"/>
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            <dbReference type="EMBL" id="AAK41004.1"/>
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            <dbReference type="PDB" id="9FHL"/>
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    <evidence type="ECO:0007744" key="9">
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            <dbReference type="PDB" id="9FRK"/>
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    <sequence length="133" mass="15064" checksum="25CF706993BF368D" modified="2000-05-01" version="1">MVFVNPLANALTSIYNNEMRRNKQAIIMPASKLVINVLRVMQKEGYVGEFEYIDDGRWGKITVQLLGRVNKCGPITPRYPLSYRQMIALPDYVRRYLPSKEIGIIIVSTSKGVMSHKEAARLRIGGVALGYVY</sequence>
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<entry dataset="Swiss-Prot" created="2003-03-25" modified="2026-01-28" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXC4</accession>
    <name>RRP4_SACS2</name>
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        <recommendedName>
            <fullName evidence="1">Exosome complex component Rrp4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rrp4</name>
        <name type="ordered locus">SSO0736</name>
        <name type="ORF">C20_026</name>
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    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="EMBO Rep." volume="4" first="889" last="893">
            <title>An exosome-like complex in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Evguenieva-Hackenberg E."/>
                <person name="Walter P."/>
                <person name="Hochleitner E."/>
                <person name="Lottspeich F."/>
                <person name="Klug G."/>
            </authorList>
            <dbReference type="PubMed" id="12947419"/>
            <dbReference type="DOI" id="10.1038/sj.embor.embor929"/>
        </citation>
        <scope>INTERACTION WITH EXOSOME</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2006" name="Mol. Microbiol." volume="62" first="1076" last="1089">
            <title>Characterization of native and reconstituted exosome complexes from the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Walter P."/>
                <person name="Klein F."/>
                <person name="Lorentzen E."/>
                <person name="Ilchmann A."/>
                <person name="Klug G."/>
                <person name="Evguenieva-Hackenberg E."/>
            </authorList>
            <dbReference type="PubMed" id="17078816"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.2006.05393.x"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH EXOSOME</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2008" name="Biochemistry" volume="47" first="13158" last="13168">
            <title>Rrp4 and Csl4 are needed for efficient degradation but not for polyadenylation of synthetic and natural RNA by the archaeal exosome.</title>
            <authorList>
                <person name="Evguenieva-Hackenberg E."/>
                <person name="Roppelt V."/>
                <person name="Finsterseifer P."/>
                <person name="Klug G."/>
            </authorList>
            <dbReference type="PubMed" id="19053279"/>
            <dbReference type="DOI" id="10.1021/bi8012214"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2010" name="FEBS Lett." volume="584" first="2931" last="2936">
            <title>The evolutionarily conserved subunits Rrp4 and Csl4 confer different substrate specificities to the archaeal exosome.</title>
            <authorList>
                <person name="Roppelt V."/>
                <person name="Klug G."/>
                <person name="Evguenieva-Hackenberg E."/>
            </authorList>
            <dbReference type="PubMed" id="20488184"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2010.05.014"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2012" name="Biochimie" volume="94" first="1578" last="1587">
            <title>Heterogeneous complexes of the RNA exosome in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Witharana C."/>
                <person name="Roppelt V."/>
                <person name="Lochnit G."/>
                <person name="Klug G."/>
                <person name="Evguenieva-Hackenberg E."/>
            </authorList>
            <dbReference type="PubMed" id="22503705"/>
            <dbReference type="DOI" id="10.1016/j.biochi.2012.03.026"/>
        </citation>
        <scope>SUBUNIT</scope>
        <scope>DOMAIN</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2007" name="EMBO Rep." volume="8" first="470" last="476">
            <title>RNA channelling by the archaeal exosome.</title>
            <authorList>
                <person name="Lorentzen E."/>
                <person name="Dziembowski A."/>
                <person name="Lindner D."/>
                <person name="Seraphin B."/>
                <person name="Conti E."/>
            </authorList>
            <dbReference type="PubMed" id="17380186"/>
            <dbReference type="DOI" id="10.1038/sj.embor.7400945"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH RRP41 AND RRP42</scope>
        <scope>SUBUNIT</scope>
        <scope>DOMAIN</scope>
    </reference>
    <reference key="9">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E8739" last="E8739">
            <title>Crystal structure of the S. solfataricus archaeal exosome reveals conformational flexibility in the RNA-binding ring.</title>
            <authorList>
                <person name="Lu C."/>
                <person name="Ding F."/>
                <person name="Ke A."/>
            </authorList>
            <dbReference type="PubMed" id="20090900"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0008739"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.41 ANGSTROMS) IN COMPLEX WITH RRP41 AND RRP42</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="10">
        <citation type="journal article" date="2012" name="Archaea" volume="2012" first="721869" last="721869">
            <title>Crystal structure of a 9-subunit archaeal exosome in pre-catalytic states of the phosphorolytic reaction.</title>
            <authorList>
                <person name="Lorentzen E."/>
                <person name="Conti E."/>
            </authorList>
            <dbReference type="PubMed" id="23319881"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH RRP41 AND RRP42</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 4 6">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Increases the RNA binding and the efficiency of RNA degradation. Confers strong poly(A) specificity to the exosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 3 5 7 8">Component of the archaeal exosome complex. Forms a trimer of Rrp4 and/or Csl4 subunits. The trimer associates with a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3 7">Contains an N-terminal domain that mediates interactions with the hexameric ring, a central S1 domain and a C-terminal KH domain. The KH domain is required for efficient RNA degradation, but is not responsible for poly(A) preference.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRP4 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99222">
        <property type="entry name" value="A99222"/>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
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        <property type="resolution" value="2.50 A"/>
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    <dbReference type="PDBsum" id="2JE6"/>
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    <dbReference type="PDBsum" id="3L7Z"/>
    <dbReference type="PDBsum" id="4BA1"/>
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    <dbReference type="PaxDb" id="273057-SSO0736"/>
    <dbReference type="EnsemblBacteria" id="AAK41032">
        <property type="protein sequence ID" value="AAK41032"/>
        <property type="gene ID" value="SSO0736"/>
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    <dbReference type="KEGG" id="sso:SSO0736"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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<entry dataset="Swiss-Prot" created="1996-10-01" modified="2026-01-28" version="161" xmlns="https://uniprot.org/uniprot">
    <accession>P50384</accession>
    <name>TRPD_SACS2</name>
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            <fullName evidence="1">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.18</ecNumber>
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        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
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    <reference key="1">
        <citation type="submission" date="1995-07" db="EMBL/GenBank/DDBJ databases">
            <title>The tryptophan operon in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Tutino M.L."/>
                <person name="Cubellis M."/>
                <person name="Sannia G."/>
                <person name="Marino G."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
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    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
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            <dbReference type="PubMed" id="11427726"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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    <reference key="3">
        <citation type="journal article" date="2001" name="Eur. J. Biochem." volume="268" first="2246" last="2252">
            <title>Purification, characterization and crystallization of thermostable anthranilate phosphoribosyltransferase from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Ivens A."/>
                <person name="Mayans O."/>
                <person name="Szadkowski H."/>
                <person name="Wilmanns M."/>
                <person name="Kirschner K."/>
            </authorList>
            <dbReference type="PubMed" id="11298741"/>
            <dbReference type="DOI" id="10.1046/j.1432-1327.2001.02102.x"/>
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        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>CRYSTALLIZATION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2002" name="EMBO J." volume="21" first="3245" last="3254">
            <title>Structural analysis of two enzymes catalysing reverse metabolic reactions implies common ancestry.</title>
            <authorList>
                <person name="Mayans O."/>
                <person name="Ivens A."/>
                <person name="Nissen L.J."/>
                <person name="Kirschner K."/>
                <person name="Wilmanns M."/>
            </authorList>
            <dbReference type="PubMed" id="12093726"/>
            <dbReference type="DOI" id="10.1093/emboj/cdf298"/>
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        <scope>X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH MAGNESIUM IONS</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2006" name="J. Biol. Chem." volume="281" first="21410" last="21421">
            <title>Structural and mutational analysis of substrate complexation by anthranilate phosphoribosyltransferase from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Marino M."/>
                <person name="Deuss M."/>
                <person name="Svergun D.I."/>
                <person name="Konarev P.V."/>
                <person name="Sterner R."/>
                <person name="Mayans O."/>
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            <dbReference type="PubMed" id="16714288"/>
            <dbReference type="DOI" id="10.1074/jbc.M601403200"/>
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        <scope>X-RAY CRYSTALLOGRAPHY (2.56 ANGSTROMS) IN COMPLEX WITH PHOSPHORIBOSYLPYROPHOSPHATE</scope>
        <scope>ANTHRANILATE AND MAGNESIUM IONS</scope>
        <scope>FUNCTION</scope>
        <scope>MUTAGENESIS OF LYS-106; HIS-107; HIS-154; ARG-164; ASP-223 AND GLU-224</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2009" name="Biochemistry" volume="48" first="5199" last="5209">
            <title>Activation of anthranilate phosphoribosyltransferase from Sulfolobus solfataricus by removal of magnesium inhibition and acceleration of product release.</title>
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                <person name="Schlee S."/>
                <person name="Deuss M."/>
                <person name="Bruning M."/>
                <person name="Ivens A."/>
                <person name="Schwab T."/>
                <person name="Hellmann N."/>
                <person name="Mayans O."/>
                <person name="Sterner R."/>
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            <dbReference type="DOI" id="10.1021/bi802335s"/>
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        <scope>X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) IN COMPLEX WITH PHOSPHORIBOSYLPYROPHOSPHATE AND MAGNESIUM IONS</scope>
        <scope>SUBUNIT</scope>
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    <comment type="function">
        <text evidence="1 2 4">Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + anthranilate</text>
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            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
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        </reaction>
    </comment>
    <comment type="cofactor">
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            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text>Binds 2 magnesium ions per monomer.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2 4">50 uM for magnesium (at 60 degrees Celsius and at pH 7.5)</KM>
            <KM evidence="2 4">40 nM for anthranilate (at 60 degrees Celsius and at pH 7.5)</KM>
            <KM evidence="2 4">160 uM for phosphoribosylpyrophosphate (at 60 degrees Celsius and at pH 7.5)</KM>
            <KM evidence="2 4">20 nM for anthranilate (at 25 degrees Celsius and at pH 7.5)</KM>
            <KM evidence="2 4">26 nM for anthranilate (at 40 degrees Celsius and at pH 7.5)</KM>
            <KM evidence="2 4">32 nM for anthranilate (at 50 degrees Celsius and at pH 7.5)</KM>
            <KM evidence="2 4">35 nM for anthranilate (at 60 degrees Celsius and at pH 7.5)</KM>
            <KM evidence="2 4">500 nM for anthranilate (at 87 degrees Celsius and at pH 7.5)</KM>
        </kinetics>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3 4 5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the anthranilate phosphoribosyltransferase family.</text>
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    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285:SF2">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000154523" description="Anthranilate phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="binding site">
        <location>
            <begin position="77"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <begin position="82"/>
            <end position="83"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 4 5">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <begin position="89"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <begin position="106"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 4 5">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency dedreases only 10-fold." evidence="4">
        <original>K</original>
        <variation>Q</variation>
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Limited effect on either affinity for anthranilate and catalytic efficiency. 300-fold decrease of the affinity for anthranilate, whereas catalytic efficiency remains nearly unchanged; when associated with A-178." evidence="4">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Limited effect on either affinity for anthranilate and catalytic efficiency." evidence="4">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Strong decrease of the affinity for anthranilate, although only a moderate 7-fold decrease in catalytic efficiency." evidence="4">
        <original>R</original>
        <variation>A</variation>
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="300-fold decrease of the affinity for anthranilate, whereas catalytic efficiency remains nearly unchanged; when associated with A-107.">
        <original>P</original>
        <variation>A</variation>
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged." evidence="4">
        <original>D</original>
        <variation>N</variation>
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged." evidence="4">
        <original>E</original>
        <variation>Q</variation>
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="3"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="75"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="90"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="99"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="104"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="112"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="118"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="144"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="150"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="155"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="161"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="172"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="177"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="186"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="turn" evidence="7">
        <location>
            <begin position="207"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="211"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="219"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="222"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="240"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="248"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="258"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="266"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="282"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="301"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="305"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="333"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11298741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12093726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="16714288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="19385665"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="1O17"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="1ZYK"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="3GBR"/>
        </source>
    </evidence>
    <sequence length="345" mass="37574" checksum="D51927F4B7AAFA90" modified="1996-10-01" version="1">MNINEILKKLINKSDLEINEAEELAKAIIRGEVPEILVSAILVALRMKGESKNEIVGFARAMRELAIKIDVPNAIDTAGTGGDGLGTVNVSTASAILLSLVNPVAKHGNRAVSGKSGSADVLEALGYNIIVPPERAKELVNKTNFVFLFAQYYHPAMKNVANVRKTLGIRTIFNILGPLTNPANAKYQLMGVFSKDHLDLLSKSAYELDFNKIILVYGEPGIDEVSPIGNTFMKIVSKRGIEEVKLNVTDFGISPIPIEKLIVNSAEDSAIKIVRAFLGKDEHVAEFIKINTAVALFALDRVGDFREGYEYADHLIEKSLDKLNEIISMNGDVTKLKTIVVKSSG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-20" modified="2025-06-18" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>P58202</accession>
    <name>PCP2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Pyrrolidone-carboxylate peptidase 2</fullName>
            <ecNumber>3.4.19.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>5-oxoprolyl-peptidase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Pyroglutamyl-peptidase I 2</fullName>
            <shortName>PGP-I 2</shortName>
            <shortName>Pyrase 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pcp2</name>
        <name type="ordered locus">SSO1607</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes 5-oxoproline from various penultimate amino acid residues except L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro.</text>
            <dbReference type="EC" id="3.4.19.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C15 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90320">
        <property type="entry name" value="C90320"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923507.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58202"/>
    <dbReference type="SMR" id="P58202"/>
    <dbReference type="FunCoup" id="P58202">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="MEROPS" id="C15.001"/>
    <dbReference type="PaxDb" id="273057-SSO1607"/>
    <dbReference type="EnsemblBacteria" id="AAK41818">
        <property type="protein sequence ID" value="AAK41818"/>
        <property type="gene ID" value="SSO1607"/>
    </dbReference>
    <dbReference type="GeneID" id="15297022"/>
    <dbReference type="KEGG" id="sso:SSO1607"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1645"/>
    <dbReference type="eggNOG" id="arCOG05850">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043960_4_3_2"/>
    <dbReference type="InParanoid" id="P58202"/>
    <dbReference type="PhylomeDB" id="P58202"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016920">
        <property type="term" value="F:pyroglutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00501">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.20:FF:000005">
        <property type="entry name" value="Pyrrolidone-carboxylate peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.20">
        <property type="entry name" value="Peptidase C15, pyroglutamyl peptidase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00417">
        <property type="entry name" value="Pyrrolid_peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000816">
        <property type="entry name" value="Peptidase_C15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016125">
        <property type="entry name" value="Peptidase_C15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036440">
        <property type="entry name" value="Peptidase_C15-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029762">
        <property type="entry name" value="PGP-I_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033694">
        <property type="entry name" value="PGPEP1_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033693">
        <property type="entry name" value="PGPEP1_Glu_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009672">
        <property type="entry name" value="PRK13193.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402">
        <property type="entry name" value="PROTEASE FAMILY C15 PYROGLUTAMYL-PEPTIDASE I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402:SF1">
        <property type="entry name" value="PYROGLUTAMYL-PEPTIDASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01470">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015592">
        <property type="entry name" value="Prld-crbxl_pptds"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00706">
        <property type="entry name" value="PYROGLUPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53182">
        <property type="entry name" value="Pyrrolidone carboxyl peptidase (pyroglutamate aminopeptidase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01334">
        <property type="entry name" value="PYRASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01333">
        <property type="entry name" value="PYRASE_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0788">Thiol protease</keyword>
    <feature type="chain" id="PRO_0000184759" description="Pyrrolidone-carboxylate peptidase 2">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="209" mass="23404" checksum="AAE205344FD024B7" modified="2001-06-20" version="1">MTVLLFGFEPFLEYKENPSQLIVEALNGSTILKEEVKGVILPVEYEKIEDLIVTKIREMKPILTLGIGVAPGRAKITPEKIAINYKYSREGDNAGKKYKGEKIDPLGQDGIFTNIPVEDLVDLLNENGIPAELSLSAGSYLCNNAMYIIIREARKYNSLGGFIHVPLHESYAARIQRPIPSMSLDTMIRGIRLSMEFILTNKKENLTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX44</accession>
    <name>Q7LX44_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">dTDP-4-dehydrorhamnose reductase (RfbD-3)</fullName>
            <ecNumber evidence="2">1.1.1.133</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rfbD-3</name>
        <name evidence="2" type="ordered locus">SSO2161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.133" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74068">
        <property type="entry name" value="S74068"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992152.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX44"/>
    <dbReference type="FunCoup" id="Q7LX44">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2161"/>
    <dbReference type="PaxDb" id="273057-SSO2161"/>
    <dbReference type="EnsemblBacteria" id="AAK42337">
        <property type="protein sequence ID" value="AAK42337"/>
        <property type="gene ID" value="SSO2161"/>
    </dbReference>
    <dbReference type="GeneID" id="1453664"/>
    <dbReference type="KEGG" id="sso:SSO2161"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2254"/>
    <dbReference type="eggNOG" id="arCOG01367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1192571_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX44"/>
    <dbReference type="PhylomeDB" id="Q7LX44"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008831">
        <property type="term" value="F:dTDP-4-dehydrorhamnose reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43242">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43242:SF1">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RmlD-like substrate binding" evidence="1">
        <location>
            <begin position="34"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="255" mass="28640" checksum="42F9A507C931B1C6" modified="2011-05-31" version="1">MKIAVTDEGEVAKAIAKFLGGKGNEIIVVDNPSKVIREKPDVIIHTFEIPIFESNANPPLAWSFNTWYAINIARAGSKAGSVNVFLSSFLIYDGKRGFYKEHNTPHPLNYYGLTKLVGESSIISLGNYLVLRVGALFSLSYRGFLFPFIKASTMGKVLKCNKNFYISIIDLNTLAKVIKLLIDKEARGVINVGSNRVSLFDICSYLSDIFGNEVIEIDNRQRDFSLDDWLLRAYNIKIDAKESILSLIEYRLLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX86</accession>
    <name>Q7LX86_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74022">
        <property type="entry name" value="S74022"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990137.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX86"/>
    <dbReference type="PaxDb" id="273057-SSO2110"/>
    <dbReference type="EnsemblBacteria" id="AAK42289">
        <property type="protein sequence ID" value="AAK42289"/>
        <property type="gene ID" value="SSO2110"/>
    </dbReference>
    <dbReference type="GeneID" id="1453616"/>
    <dbReference type="KEGG" id="sso:SSO2110"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2196"/>
    <dbReference type="eggNOG" id="arCOG09933">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_712937_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX86"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="45165" checksum="AD90D5BFEF5207BC" modified="2011-05-31" version="1">MDFKEVEELTRGLSAYERRFAEIYYYLYRASENILTKDELDEYYKILKRRDHSADHLVKLAEVYLIMGDKDTMSIILQKNKRIVEDKVLVSNTLILLECLSGRKPTYSKLALMGVIAECSHLLEDYDPMEYFMRLLRDNPSYNTESNISEFLRSIAIRFDKEPARSELVEDALMLNERVKREKTEKILNNYTLAVALRGLGRIKESEKFVESLREGLKKYDYEFYFSAHSLVSYHSIFNEIDEVDKLIDSIERIKHGDKTTNSMMRALSANTAYIYTNKERYLDIALEAFQKLKGDVKINVGIIFLESVDKPDILFNIINEITAESNYLFYLDEISSSLGIAYANIKDNRILELMNNAPFYRFIFEFILSMAGQSVSNRLKISLSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXG9</accession>
    <name>Q7LXG9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PaREP1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0804</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90230">
        <property type="entry name" value="H90230"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923098.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXG9"/>
    <dbReference type="SMR" id="Q7LXG9"/>
    <dbReference type="PaxDb" id="273057-SSO0804"/>
    <dbReference type="DNASU" id="1455070"/>
    <dbReference type="EnsemblBacteria" id="AAK41103">
        <property type="protein sequence ID" value="AAK41103"/>
        <property type="gene ID" value="SSO0804"/>
    </dbReference>
    <dbReference type="GeneID" id="1455070"/>
    <dbReference type="KEGG" id="sso:SSO0804"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.814"/>
    <dbReference type="eggNOG" id="arCOG03712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118419_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXG9"/>
    <dbReference type="PhylomeDB" id="Q7LXG9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="20199" checksum="016EC98AA550249B" modified="2011-05-31" version="1">MESKIPAIEKHRDAYVKIRIIESLDELALGLKLLKEGFSRNSANKVFQSWKAIISALTVINLEKMPRNEKEKEWYYKSGFLAPTTGLKGISQRLEELGYKVNSLTSVALELHRYAYNGLYKGASDYYEREEAINDIIYLSKEIMKVIKEFFKEYWDEEIEEHYNLAEKELMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-03-27" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXH8</accession>
    <name>Q7LXH8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4935 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0795</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90229">
        <property type="entry name" value="F90229"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXH8"/>
    <dbReference type="STRING" id="273057.SSO0795"/>
    <dbReference type="PaxDb" id="273057-SSO0795"/>
    <dbReference type="EnsemblBacteria" id="AAK41093">
        <property type="protein sequence ID" value="AAK41093"/>
        <property type="gene ID" value="SSO0795"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0795"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.801"/>
    <dbReference type="eggNOG" id="arCOG07186">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_945338_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXH8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="34518" checksum="00C0298C70D828A9" modified="2011-05-31" version="1">MNMETFFLDTNILVKWIYRKLLNEKEDIERFISSLSKDQCIILDINLSEFESIINDAYNMVSHIIYEKILSRSDWKELDIKGKFQIINDIRKNFDRLYDEIRKTHYNELISPGGIRQILAKEFFKKLEDKVVNLDPEVLRNHIALNGRTEDLIDYLSSVKAIIKQKFTVLDNVKILINDYVIKEIQLIYEGINRHLRNLKSQGYKKVPSRKDQLIFQYLFLLLREGIYDNIVFITDDNDFERMYRAILNYSDDIIKGKVDPRGEFRGHAMEIKNTLGKLVIKNINELISNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXK3</accession>
    <name>Q7LXK3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0762</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90225">
        <property type="entry name" value="D90225"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991350.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXK3"/>
    <dbReference type="STRING" id="273057.SSO0762"/>
    <dbReference type="PaxDb" id="273057-SSO0762"/>
    <dbReference type="EnsemblBacteria" id="AAK41059">
        <property type="protein sequence ID" value="AAK41059"/>
        <property type="gene ID" value="SSO0762"/>
    </dbReference>
    <dbReference type="GeneID" id="1455026"/>
    <dbReference type="KEGG" id="sso:SSO0762"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.761"/>
    <dbReference type="eggNOG" id="arCOG05948">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1567236_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXK3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="162" mass="18893" checksum="6092FF766DB9625D" modified="2011-05-31" version="1">MNCIENLHKAYILTWIGDYKTASELARECMQLLSDSVEIRRKVKEVLKKADREYKISKKLREESINTTDLIQVALYYLAKRLSVKKDNDIEIIEKGNIKLSVIEHLLVKEVRGYCEGCRGYKYSLLTSAKGYLILYDEIIYAEFFEGNKDDVINEILKNTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXM5</accession>
    <name>Q7LXM5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CARDB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0687</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90216">
        <property type="entry name" value="D90216"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991233.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXM5"/>
    <dbReference type="STRING" id="273057.SSO0687"/>
    <dbReference type="PaxDb" id="273057-SSO0687"/>
    <dbReference type="EnsemblBacteria" id="AAK40987">
        <property type="protein sequence ID" value="AAK40987"/>
        <property type="gene ID" value="SSO0687"/>
    </dbReference>
    <dbReference type="GeneID" id="1454954"/>
    <dbReference type="KEGG" id="sso:SSO0687"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.686"/>
    <dbReference type="eggNOG" id="arCOG02089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_335136_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXM5"/>
    <dbReference type="PhylomeDB" id="Q7LXM5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902:SF3">
        <property type="entry name" value="NPCBM-ASSOCIATED, NEW3 DOMAIN OF ALPHA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902">
        <property type="entry name" value="S-LAYER DOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="824"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="851" mass="92113" checksum="7868A56C07867254" modified="2011-05-31" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXM8</accession>
    <name>Q7LXM8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamine amidotransferase type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0683</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99216">
        <property type="entry name" value="A99216"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991230.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXM8"/>
    <dbReference type="STRING" id="273057.SSO0683"/>
    <dbReference type="PaxDb" id="273057-SSO0683"/>
    <dbReference type="EnsemblBacteria" id="AAK40984">
        <property type="protein sequence ID" value="AAK40984"/>
        <property type="gene ID" value="SSO0683"/>
    </dbReference>
    <dbReference type="GeneID" id="1454951"/>
    <dbReference type="KEGG" id="sso:SSO0683"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.683"/>
    <dbReference type="eggNOG" id="arCOG00095">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_424917_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXM8"/>
    <dbReference type="PhylomeDB" id="Q7LXM8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00981">
        <property type="entry name" value="arch_gltB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01907">
        <property type="entry name" value="GlxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="entry name" value="Glutamate synthase, alpha subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035710">
        <property type="entry name" value="Archaeal_gltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012075">
        <property type="entry name" value="Glu_synth_lsu_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39673">
        <property type="entry name" value="TUNGSTEN FORMYLMETHANOFURAN DEHYDROGENASE, SUBUNIT C (FWDC)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39673:SF5">
        <property type="entry name" value="TUNGSTEN-CONTAINING FORMYLMETHANOFURAN DEHYDROGENASE 2 SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00310">
        <property type="entry name" value="GATase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036632">
        <property type="entry name" value="GOGAT_lg_1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="Alpha subunit of glutamate synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="1">
        <location>
            <begin position="9"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="659" mass="74322" checksum="219BE8278F967EDA" modified="2011-05-31" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXP5</accession>
    <name>Q7LXP5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zn-dependent protease, tldE protein homolog, putative (TldE)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tldE</name>
        <name evidence="2" type="ordered locus">SSO0661</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90213">
        <property type="entry name" value="G90213"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991204.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXP5"/>
    <dbReference type="STRING" id="273057.SSO0661"/>
    <dbReference type="PaxDb" id="273057-SSO0661"/>
    <dbReference type="EnsemblBacteria" id="AAK40966">
        <property type="protein sequence ID" value="AAK40966"/>
        <property type="gene ID" value="SSO0661"/>
    </dbReference>
    <dbReference type="GeneID" id="1454933"/>
    <dbReference type="KEGG" id="sso:SSO0661"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.663"/>
    <dbReference type="eggNOG" id="arCOG00322">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_4_2_2"/>
    <dbReference type="InParanoid" id="Q7LXP5"/>
    <dbReference type="PhylomeDB" id="Q7LXP5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905368">
        <property type="term" value="C:peptidase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="entry name" value="PmbA/TldD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047657">
        <property type="entry name" value="PmbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43421">
        <property type="entry name" value="METALLOPROTEASE PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43421:SF1">
        <property type="entry name" value="METALLOPROTEASE PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="1">
        <location>
            <begin position="202"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="419" mass="47375" checksum="2D9F09B4D0E3F230" modified="2011-05-31" version="1">MYERLISRAKAMGISLEIFSVKMREYTIAKDKFYVPIIVEETGYGIRLIDEKYRIGYIYTKKLNDNVLEEALKIAKLGESDKANILPSKQPVKRINENQINEDVVQDTIKDLIELEEKVNLTSASASAINYEVSIFNTEGVEVSEKRGVLSVQASANDGNNSPEIYEYLIKRDSKISLEKLKASIIEKVKIMKKKERIDPKGYDVTFTQKALDSLFSSLFPSLFSARNFYKETSPFKLGELVNENLEIIDDPLNQSLPFSRSFDDEGNPSRVSKVIESGLVKRPLTNTYWATRMSVENTSSASRNLDWVVPSYFIPPHVDFSNIIFDYKNTESNIEENSVIVDQLEGIDTADLSSGNFSLVANVSWLNRKDEKIGLEELLITGNLKQLLKNIVSTSKNIENYGKVVSGKLKVRGLSLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXQ1</accession>
    <name>Q7LXQ1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Enoyl CoA hydratase (PaaF-2)</fullName>
            <ecNumber evidence="4">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">paaF-2</name>
        <name evidence="4" type="ordered locus">SSO0654</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90212">
        <property type="entry name" value="H90212"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991192.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXQ1"/>
    <dbReference type="FunCoup" id="Q7LXQ1">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0654"/>
    <dbReference type="PaxDb" id="273057-SSO0654"/>
    <dbReference type="EnsemblBacteria" id="AAK40959">
        <property type="protein sequence ID" value="AAK40959"/>
        <property type="gene ID" value="SSO0654"/>
    </dbReference>
    <dbReference type="GeneID" id="1454926"/>
    <dbReference type="KEGG" id="sso:SSO0654"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.656"/>
    <dbReference type="eggNOG" id="arCOG00239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_2"/>
    <dbReference type="InParanoid" id="Q7LXQ1"/>
    <dbReference type="PhylomeDB" id="Q7LXQ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000009">
        <property type="entry name" value="Carnitinyl-CoA dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="252" mass="27981" checksum="16738B14E23CC2E6" modified="2011-05-31" version="1">METIVTKEEPPIEWIVLNRPDKLNSINSIMIREIGDILRQIVDNDKIKVVIFTGSGKAFSAGADISQFKDLDPVKAWEFAIKGRELMDYIERYPKPTIAMINGYALGGGLELALSCDLRIASQIAELGLPEINLGIYPGFGGTQRLVRLIGKGRAMEIIMLGDIIKADYAERIGLVNRVVEPSSLEKEVRELALKLAEKPQIAIRLIKTVVNYGNDAPILSGLTLESLGWGIAFATEEEKKRVNEFLSRRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXT7</accession>
    <name>Q7LXT7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Magnesium-dependent phosphatase-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90204">
        <property type="entry name" value="F90204"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXT7"/>
    <dbReference type="FunCoup" id="Q7LXT7">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0580"/>
    <dbReference type="PaxDb" id="273057-SSO0580"/>
    <dbReference type="EnsemblBacteria" id="AAK40893">
        <property type="protein sequence ID" value="AAK40893"/>
        <property type="gene ID" value="SSO0580"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0580"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.588"/>
    <dbReference type="eggNOG" id="arCOG04046">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1648366_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXT7"/>
    <dbReference type="PhylomeDB" id="Q7LXT7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010033">
        <property type="entry name" value="HAD_SF_ppase_IIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010036">
        <property type="entry name" value="MDP_1_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051540">
        <property type="entry name" value="S-2-haloacid_dehalogenase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01681">
        <property type="entry name" value="HAD-SF-IIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01685">
        <property type="entry name" value="MDP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316:SF8">
        <property type="entry name" value="HAD FAMILY HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316">
        <property type="entry name" value="HYDROLASE, HALOACID DELAHOGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12689">
        <property type="entry name" value="Acid_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20878" checksum="31CD218818D84E6A" modified="2011-05-31" version="1">MLIILFLLVYTEMIRAIVFDADKTLWDHHNISEFEEPLRLIDSNTLEDSKGRVLHLFPDVKETLKELKNRGYILGLATWNFEDKAIKVLAALDLLQYFDIIVAKPYPYKFLMLSQIIVEINTKANQKIKPSEVLFLDDRRGHFGNIWLYLGNVKCLEMWKDISKYSEIFSVLSHVNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LYJ7</accession>
    <name>Q7LYJ7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Archaeal Rieske-type ferredoxin (Arf)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">arf</name>
        <name evidence="7" type="ordered locus">SSO2080</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74009">
        <property type="entry name" value="S74009"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989803.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LYJ7"/>
    <dbReference type="STRING" id="273057.SSO2080"/>
    <dbReference type="PaxDb" id="273057-SSO2080"/>
    <dbReference type="EnsemblBacteria" id="AAK42261">
        <property type="protein sequence ID" value="AAK42261"/>
        <property type="gene ID" value="SSO2080"/>
    </dbReference>
    <dbReference type="GeneID" id="1453588"/>
    <dbReference type="KEGG" id="sso:SSO2080"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2158"/>
    <dbReference type="eggNOG" id="arCOG02852">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_5_3_2"/>
    <dbReference type="InParanoid" id="Q7LYJ7"/>
    <dbReference type="PhylomeDB" id="Q7LYJ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03467">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496">
        <property type="entry name" value="FERREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496:SF0">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="6">
        <location>
            <begin position="3"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="108" mass="12509" checksum="5D2BDE6B0357D2EA" modified="2011-05-31" version="1">MLVRVSSLSDLEEKKPRKFSINGIEVVIVRVGDKVFAIEAYCPHKGRNLEYGEVEGYKIRCDLHGYEYSLENGELIFNPYGKTSGWYFSPNLRIYKVEIKGKDIFIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TW8</accession>
    <name>Q97TW8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Second ORF in transposon ISC1048</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1706</name>
        <name evidence="3" type="ordered locus">SSO1793</name>
        <name evidence="4" type="ordered locus">SSO2925</name>
        <name evidence="5" type="ordered locus">SSO2931</name>
        <name evidence="6" type="ordered locus">SSO2947</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90472">
        <property type="entry name" value="F90472"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TW8"/>
    <dbReference type="STRING" id="273057.SSO1706"/>
    <dbReference type="PaxDb" id="273057-SSO1706"/>
    <dbReference type="EnsemblBacteria" id="AAK41912">
        <property type="protein sequence ID" value="AAK41912"/>
        <property type="gene ID" value="SSO1706"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41986">
        <property type="protein sequence ID" value="AAK41986"/>
        <property type="gene ID" value="SSO1793"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK43032">
        <property type="protein sequence ID" value="AAK43032"/>
        <property type="gene ID" value="SSO2925"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK43037">
        <property type="protein sequence ID" value="AAK43037"/>
        <property type="gene ID" value="SSO2931"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK43050">
        <property type="protein sequence ID" value="AAK43050"/>
        <property type="gene ID" value="SSO2947"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1706"/>
    <dbReference type="KEGG" id="sso:SSO1793"/>
    <dbReference type="KEGG" id="sso:SSO2925"/>
    <dbReference type="KEGG" id="sso:SSO2931"/>
    <dbReference type="KEGG" id="sso:SSO2947"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1756"/>
    <dbReference type="eggNOG" id="arCOG02130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056788_15_2_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="6"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="175" mass="20261" checksum="0CE34A75AEB1537E" modified="2001-10-01" version="1">MERKALFFLDECRTVISTSIKKVLAKVGSKPVMRVNIGFSSIYVILAINAWTGEVVVSLAKRPNSESVKYFLRYFKRRVGSGRVYMVMDNYSPHKTKGTLEVCRRKGIHPVFTPPYSPELNMAEAVFKSLKNYMSNKIFYTIEDVKNCIKQFFEENKYRFNLNAITYLGLDKIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TY4</accession>
    <name>Q97TY4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3248</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90510">
        <property type="entry name" value="E90510"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989658.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TY4"/>
    <dbReference type="STRING" id="273057.SSO3248"/>
    <dbReference type="PaxDb" id="273057-SSO3248"/>
    <dbReference type="EnsemblBacteria" id="AAK43342">
        <property type="protein sequence ID" value="AAK43342"/>
        <property type="gene ID" value="SSO3248"/>
    </dbReference>
    <dbReference type="GeneID" id="1453259"/>
    <dbReference type="KEGG" id="sso:SSO3248"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3347"/>
    <dbReference type="eggNOG" id="arCOG05379">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1682781_0_0_2"/>
    <dbReference type="InParanoid" id="Q97TY4"/>
    <dbReference type="PhylomeDB" id="Q97TY4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018632">
        <property type="entry name" value="AAA-associated_dom_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09821">
        <property type="entry name" value="AAA_assoc_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="156" mass="17917" checksum="7A0D078880C5AC03" modified="2001-10-01" version="1">MEVIDPHARVADLVGLLYSLENTFNGKTDLYMLEKEMEVDLDDLMPIVYTANSLGFITVGEGDIIITDKGIEFLKSNLKRRKEIIKDSIKKIEPFKTTLELKYFTIEELKYVLEKKGIQVYNSPEGLYDLQITLVEWGVYSGLLKKEGERFIVVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TY6</accession>
    <name>Q97TY6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PaREP1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90510">
        <property type="entry name" value="C90510"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989664.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TY6"/>
    <dbReference type="STRING" id="273057.SSO3246"/>
    <dbReference type="PaxDb" id="273057-SSO3246"/>
    <dbReference type="EnsemblBacteria" id="AAK43338">
        <property type="protein sequence ID" value="AAK43338"/>
        <property type="gene ID" value="SSO3246"/>
    </dbReference>
    <dbReference type="GeneID" id="1453257"/>
    <dbReference type="KEGG" id="sso:SSO3246"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3345"/>
    <dbReference type="eggNOG" id="arCOG03721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2032964_0_0_2"/>
    <dbReference type="InParanoid" id="Q97TY6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="14209" checksum="E9312A576B4A6E52" modified="2001-10-01" version="1">METLNESKKEFYTYFISTSKFYYDLSNTVNSPIVVCEMLYEAINAGIKLLSYYFSLQDKPRTEVVKELSSILGDWVEYYWNLGLTLHYDCYLSGNVDEDDIPLYENQVKDFISRVEEVVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U10</accession>
    <name>Q97U10_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90507">
        <property type="entry name" value="A90507"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U10"/>
    <dbReference type="STRING" id="273057.SSO3215"/>
    <dbReference type="PaxDb" id="273057-SSO3215"/>
    <dbReference type="EnsemblBacteria" id="AAK43312">
        <property type="protein sequence ID" value="AAK43312"/>
        <property type="gene ID" value="SSO3215"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3215"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3318"/>
    <dbReference type="eggNOG" id="arCOG05925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165878_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U10"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="129" mass="15006" checksum="F184726CEFBD2A93" modified="2001-10-01" version="1">MVEHKIFITIMEFPAEVRNNLSDGLCLTCCNDSVVCMSEDYPKNANVEVLFEIDREGREVIFRHIIMDDPSNPLTVEYGVDAKFVENVSQKKWIDIYFVNHSFNVEIKLRITFSDNEIRVMRREIGLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U25</accession>
    <name>Q97U25_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF123 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90505">
        <property type="entry name" value="B90505"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991683.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U25"/>
    <dbReference type="STRING" id="273057.SSO3200"/>
    <dbReference type="PaxDb" id="273057-SSO3200"/>
    <dbReference type="EnsemblBacteria" id="AAK43297">
        <property type="protein sequence ID" value="AAK43297"/>
        <property type="gene ID" value="SSO3200"/>
    </dbReference>
    <dbReference type="GeneID" id="1453216"/>
    <dbReference type="KEGG" id="sso:SSO3200"/>
    <dbReference type="eggNOG" id="arCOG00463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115699_2_0_2"/>
    <dbReference type="InParanoid" id="Q97U25"/>
    <dbReference type="PhylomeDB" id="Q97U25"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd10441">
        <property type="entry name" value="GIY-YIG_COG1833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002837">
        <property type="entry name" value="DUF123"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37460">
        <property type="entry name" value="ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37460:SF1">
        <property type="entry name" value="ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01986">
        <property type="entry name" value="DUF123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="14774" checksum="BA0D49A2B9C108D4" modified="2001-10-01" version="1">MKSYILLIKCKTDTTIRTKSKKFLIRKGVYAYVGSCGRSCGRRVSRHFNKEKSNYHWHIDYLTKACEPIGVFVMNHVGEKELALLFSKNNAFIEEFGSSDDKDAKSHLFIVNDLQELCSTVCDKSRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U57</accession>
    <name>Q97U57_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90501">
        <property type="entry name" value="B90501"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924152.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U57"/>
    <dbReference type="PaxDb" id="273057-SSO3166"/>
    <dbReference type="EnsemblBacteria" id="AAK43265">
        <property type="protein sequence ID" value="AAK43265"/>
        <property type="gene ID" value="SSO3166"/>
    </dbReference>
    <dbReference type="GeneID" id="1453184"/>
    <dbReference type="KEGG" id="sso:SSO3166"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3270"/>
    <dbReference type="HOGENOM" id="CLU_840967_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U57"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="292" mass="33811" checksum="1BCD9BA8F55389D0" modified="2001-10-01" version="1">MIRAEIPRSLYETFQEAIKDTVISDMGDVLLVSLDDSLIEIKGYISSVISNTTFYYGEVKLCEIFDLNEVHDSINQGLYKFNDYKESVKYCLFSISKFVKERSIEVTSNRFLYVRSEIFSVVNKIIEENYPVLGRNKIEDQVQYLIKSNDKTVILSVVVSLMESKKIYAFANVEDDSDAEQVAILFYDRIYYIDKLKFDTVDNIETGINKIVDYIINYDESRMEGADDYYLLLTINSVGTLMERFTIIRNIIDSINDMVSDINLRINFGYFNGDFDLKLIKGEDIVSIGKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U64</accession>
    <name>Q97U64_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Lipoyl-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO12093</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90500">
        <property type="entry name" value="C90500"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989966.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U64"/>
    <dbReference type="STRING" id="273057.SSO12093"/>
    <dbReference type="PaxDb" id="273057-SSO12093"/>
    <dbReference type="EnsemblBacteria" id="AAK43258">
        <property type="protein sequence ID" value="AAK43258"/>
        <property type="gene ID" value="SSO12093"/>
    </dbReference>
    <dbReference type="GeneID" id="1453177"/>
    <dbReference type="KEGG" id="sso:SSO12093"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3263"/>
    <dbReference type="eggNOG" id="arCOG02700">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2406489_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U64"/>
    <dbReference type="PhylomeDB" id="Q97U64"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="2">
        <location>
            <begin position="2"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="81" mass="9059" checksum="C0C9C3EB25B268C5" modified="2001-10-01" version="1">MQAELKIPEDIWPRRRDWGGEIVSLYIKEGSEIKVGDVIAEVEIEKAILKILSQYNGKVIKVLVREGDKVSPGSVIALIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U80</accession>
    <name>Q97U80_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Permease, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO3143</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90498">
        <property type="entry name" value="B90498"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990911.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U80"/>
    <dbReference type="FunCoup" id="Q97U80">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3143"/>
    <dbReference type="EnsemblBacteria" id="AAK43241">
        <property type="protein sequence ID" value="AAK43241"/>
        <property type="gene ID" value="SSO3143"/>
    </dbReference>
    <dbReference type="GeneID" id="1453160"/>
    <dbReference type="KEGG" id="sso:SSO3143"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3247"/>
    <dbReference type="eggNOG" id="arCOG02688">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_2"/>
    <dbReference type="InParanoid" id="Q97U80"/>
    <dbReference type="PhylomeDB" id="Q97U80"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF34">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="19"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK43241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="448" mass="50013" checksum="3F7580ABD568CB6C" modified="2001-10-01" version="1">MSIAGRIERLPWTSFHTRLLAILTLGEFFELYDLFTGGFVASQIASFYKVSSATGIYYTVAIFFLGAFVGAIIFNYIGDSVGRRTALIINMTITSIALLLIPFSPNILTLGILRFLAGVGVGPEAILVLDVLVTEFFPSNVRGKALAIGYTIAWTAPIVVAVLAYFLLPHKYFLYGWQWLFIIGGLGILTIIPFRFLIPESPRWLESKGRLDEAEKTVRKMEEIAIKEKGQLGEPIQVQVITSQRVKISELFSPLYRKRTVMLWIFEFLQAGVYYGFASLAPSVLFAKGFTLVHTLQYTMIIYTSYFISSLASIFIIDSQKFDRKWQVSIVMLLMGLVGLAFGFSSSPLEAVVTGFTFGFLSNIFSNAFHQYGAELYPTRVRAFADGVQYSLSRLGNYIWLTLLPIVLYSNGPVAMYTIVFIMALIVTLDVAILGPKASKIELEELSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U82</accession>
    <name>Q97U82_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90497">
        <property type="entry name" value="H90497"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990915.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U82"/>
    <dbReference type="STRING" id="273057.SSO3141"/>
    <dbReference type="PaxDb" id="273057-SSO3141"/>
    <dbReference type="DNASU" id="1453158"/>
    <dbReference type="EnsemblBacteria" id="AAK43239">
        <property type="protein sequence ID" value="AAK43239"/>
        <property type="gene ID" value="SSO3141"/>
    </dbReference>
    <dbReference type="GeneID" id="1453158"/>
    <dbReference type="KEGG" id="sso:SSO3141"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3245"/>
    <dbReference type="eggNOG" id="arCOG03855">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1478902_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U82"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="187" mass="20340" checksum="18E4D4AEEF18DEA5" modified="2001-10-01" version="1">MPSEVTEEFIIIIAVVLIGLVLFGFVTSYFIPHEIFSLAQQQASSISSSTTVSVGPLIFNSGSASTVIEAYNPAISGNITLIVFPEPSYLQQDVGLVTPQSPPQFSVYLSNEKLAKSFTISIPIYDVNGRIIYDSQITAYTVPFNTPVTITVNGVGGSNYILIVWVLYNSNGYWFRIGYTFTGVPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U90</accession>
    <name>Q97U90_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Iron-sulfur protein, conserved putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO3130</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90496">
        <property type="entry name" value="H90496"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U90"/>
    <dbReference type="FunCoup" id="Q97U90">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3130"/>
    <dbReference type="PaxDb" id="273057-SSO3130"/>
    <dbReference type="EnsemblBacteria" id="AAK43231">
        <property type="protein sequence ID" value="AAK43231"/>
        <property type="gene ID" value="SSO3130"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3130"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3236"/>
    <dbReference type="eggNOG" id="arCOG00335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027059_3_0_2"/>
    <dbReference type="InParanoid" id="Q97U90"/>
    <dbReference type="PhylomeDB" id="Q97U90"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003741">
        <property type="entry name" value="LUD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004452">
        <property type="entry name" value="LutB/LldF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47153">
        <property type="entry name" value="LACTATE UTILIZATION PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47153:SF2">
        <property type="entry name" value="LACTATE UTILIZATION PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02589">
        <property type="entry name" value="LUD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="290"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="384" mass="43003" checksum="25C7BA78DA7F7A98" modified="2001-10-01" version="1">MVNDWDIAIKRGVEHNVPRVFKVLREHPYLEDLARKVREGKLEVLSNLDYYIEMTMKSVERIGGKAYFVENAEQAREIVGKIVGNGKRVVMGKSMVAYETGIRKYLQSLGNEVWETDLGELLIQFADEPPSHIIAPAVHMTRERIRELIKEKLGVDPGDKHEDIVKVAREFLRQKFLTADIGITGANAVAADAGSIVLVENEGNIRMSSVVPPVHISITGVEKILPNLEYAIYEALVQAAYAGLYPPTYINVTSGPSSTGDVEQKRVSPAHGPKEFHLILVDNGRRKANEDPVLREALLCIRCGRCHFHCPVYRVLDGKWGDPPYSGPMGAMWSGIVYNDYKPALLCSHAGNCREVCPMKINIPKVLEYLKNKYFSMMRAGTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UD8</accession>
    <name>Q97UD8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO3079</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90490">
        <property type="entry name" value="H90490"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989097.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UD8"/>
    <dbReference type="FunCoup" id="Q97UD8">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3079"/>
    <dbReference type="PaxDb" id="273057-SSO3079"/>
    <dbReference type="EnsemblBacteria" id="AAK43183">
        <property type="protein sequence ID" value="AAK43183"/>
        <property type="gene ID" value="SSO3079"/>
    </dbReference>
    <dbReference type="GeneID" id="1453102"/>
    <dbReference type="KEGG" id="sso:SSO3079"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3185"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UD8"/>
    <dbReference type="PhylomeDB" id="Q97UD8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17319">
        <property type="entry name" value="MFS_ExuT_GudP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000870">
        <property type="entry name" value="MFS transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000126">
        <property type="entry name" value="MFS transporter permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF36">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G08340)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="18"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK43183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="423" mass="46520" checksum="841D409AEE5491C2" modified="2001-10-01" version="1">MRYRSLVETSRSKRYVRLLPMLFFLYIVNFLDRVNISYAIDAGMFQYLGVSAKEVGIIASLASSLFFVGYFIPQIFSNLGINRYGVRKIFLIAFLAWGIITIATGFVTSVSQVYVLRFLLGIAEGPFFAGVMFYLSLWFLKPERATANSFFTAAIPISGIFGSLIAGAIFATYGNYPGWRYLFWYEGMLAIFGGLLAYFILTDFPSDAKWLSNDERSALEQAFKEEEVEKVKVSWTKALADLNVILLAIVYFLGVTSLYGYSIWLPSIISFIGKVNATIASFLSIVPYVIASISLILIARYADRRQNHRFVTFAVFLVAGIGLALSAATQSIFILSFMLFAIAAIGIYSFIATFWAVPQGYLSGDAAAAAIGLINAIGNLGGIAGPIVVGFLKSYTGSFVDGIYVMALFSILAGVITLLIRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UK1</accession>
    <name>Q97UK1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90482">
        <property type="entry name" value="A90482"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992664.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UK1"/>
    <dbReference type="STRING" id="273057.SSO3010"/>
    <dbReference type="PaxDb" id="273057-SSO3010"/>
    <dbReference type="EnsemblBacteria" id="AAK43112">
        <property type="protein sequence ID" value="AAK43112"/>
        <property type="gene ID" value="SSO3010"/>
    </dbReference>
    <dbReference type="GeneID" id="1453031"/>
    <dbReference type="KEGG" id="sso:SSO3010"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3102"/>
    <dbReference type="eggNOG" id="arCOG00138">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062161_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UK1"/>
    <dbReference type="PhylomeDB" id="Q97UK1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052528">
        <property type="entry name" value="Sugar_transport-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23526">
        <property type="entry name" value="INTEGRAL MEMBRANE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23526:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="345"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="42218" checksum="74823547B08CB343" modified="2001-10-01" version="1">MKTLRLYSLLNNLAISLVNPFISFFTASNGITGALLAIASSANTAFPGIIQYALANLYIKAKKLITIGSLFGGLLWILIGVYMIYNEYFVLVYSIITAVLGAANFGWLLILDKISTTHRGRTLAYYNFYASIGGLIATLITGFIVGNNLDLMRYFFIIAGLIYVFNAYVIYNSDVDAEFARGNGIRLFSSNLEVRKFILTNFIFTFVWSMAWPIFPLAQVYKFHMNEFQVAIINVTGGTSTLALQRLVGRLVDRHRKYVMFFGRFALATFPLAYAFSTTPYEIYIANLVSGFTNSASISYTAFLFDHSSYYEKRINIALYNMFNGIAALLGSTISSVMFNVISDWFSLVVAINIMLIGIGVMRILMSLLYLKIKEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V17</accession>
    <name>Q97V17_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ferredoxin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">zfx-like2</name>
        <name evidence="11" type="ordered locus">SSO11231</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be a 3Fe-4S cluster-containing protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">To ferredoxins from P.putida and C.tartarivorum, ferredoxin I from A.vinelandii, ferredoxin II from D.desulfuricans.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90459">
        <property type="entry name" value="C90459"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989024.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V17"/>
    <dbReference type="STRING" id="273057.SSO11231"/>
    <dbReference type="PaxDb" id="273057-SSO11231"/>
    <dbReference type="EnsemblBacteria" id="AAK42930">
        <property type="protein sequence ID" value="AAK42930"/>
        <property type="gene ID" value="SSO11231"/>
    </dbReference>
    <dbReference type="GeneID" id="1452849"/>
    <dbReference type="KEGG" id="sso:SSO11231"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2905"/>
    <dbReference type="eggNOG" id="arCOG01984">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163428_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V17"/>
    <dbReference type="PhylomeDB" id="Q97V17"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007859">
        <property type="entry name" value="ETF-QO/FixX_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012206">
        <property type="entry name" value="Fd_FixX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43082">
        <property type="entry name" value="FERREDOXIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43082:SF3">
        <property type="entry name" value="FERREDOXIN-LIKE PROTEIN YDIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05187">
        <property type="entry name" value="Fer4_ETF_QO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036548">
        <property type="entry name" value="Fdx_FixX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="55"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK42930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="95" mass="10999" checksum="2D3D1148BF1FB40E" modified="2001-10-01" version="1">MRIEEKLYTLRYKKDEQPHLAIIDPNKCLRCEQVNGVPQPCIAVCPANVYSWIDQRIVISYENCVECGACRIACPYSNILWKYPRYGLGIALRYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V57</accession>
    <name>Q97V57_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2778</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90454">
        <property type="entry name" value="A90454"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990218.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V57"/>
    <dbReference type="FunCoup" id="Q97V57">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2778"/>
    <dbReference type="PaxDb" id="273057-SSO2778"/>
    <dbReference type="EnsemblBacteria" id="AAK42888">
        <property type="protein sequence ID" value="AAK42888"/>
        <property type="gene ID" value="SSO2778"/>
    </dbReference>
    <dbReference type="GeneID" id="1452807"/>
    <dbReference type="KEGG" id="sso:SSO2778"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2860"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_16_2_2"/>
    <dbReference type="InParanoid" id="Q97V57"/>
    <dbReference type="PhylomeDB" id="Q97V57"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF25">
        <property type="entry name" value="USPA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="139" mass="15161" checksum="E54F91F15A7F50D8" modified="2001-10-01" version="1">MKRILVGYDGSENAERALDFAIELASKFSARLFVVEVIDLTLFYNTGILPPLEATKSLEERAKKDVKRAIEKAKSKGVNAEGITVEGDPANSILQFATDNQIDVIVIGSRGLSKVQRIFLGSVSNKIVQESKVPVVVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VA2</accession>
    <name>Q97VA2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Type-5 uracil-DNA glycosylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">SSO2733</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 5 (UDGb) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90448">
        <property type="entry name" value="D90448"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990286.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VA2"/>
    <dbReference type="STRING" id="273057.SSO2733"/>
    <dbReference type="PaxDb" id="273057-SSO2733"/>
    <dbReference type="EnsemblBacteria" id="AAK42843">
        <property type="protein sequence ID" value="AAK42843"/>
        <property type="gene ID" value="SSO2733"/>
    </dbReference>
    <dbReference type="GeneID" id="1452762"/>
    <dbReference type="KEGG" id="sso:SSO2733"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2812"/>
    <dbReference type="eggNOG" id="arCOG00905">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083279_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VA2"/>
    <dbReference type="PhylomeDB" id="Q97VA2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033958">
        <property type="term" value="F:DNA-deoxyinosine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10031">
        <property type="entry name" value="UDG-F5_TTUDGB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051536">
        <property type="entry name" value="UDG_Type-4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044147">
        <property type="entry name" value="UdgB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693">
        <property type="entry name" value="TYPE-5 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693:SF3">
        <property type="entry name" value="TYPE-5 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="10">
        <location>
            <begin position="34"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK42843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="219" mass="24546" checksum="384FCFBB4051EF3C" modified="2001-10-01" version="1">MENFISRLIACDKCPRLTQYRKSFPDNYWKKPVPPNGQIDAEIVIVGLAPAGNGGNRTGRMFTGDESSNNLANALYAVGLSNQPFSVSKDDGLKLFNVYITSAVKCAPPQNKPNKDEIINCSVFLEEEVRILKNTKVYIALGKIAWDSLIYVFKKIGYNVPNVRFYHGALVKVVKPDMSIIWLVGSYHPSPRNMKTGRLTINMLIEIFNTAKMLVNTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VC3</accession>
    <name>Q97VC3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YkgJ family cysteine cluster protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2711</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90445">
        <property type="entry name" value="G90445"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988640.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VC3"/>
    <dbReference type="STRING" id="273057.SSO2711"/>
    <dbReference type="PaxDb" id="273057-SSO2711"/>
    <dbReference type="EnsemblBacteria" id="AAK42822">
        <property type="protein sequence ID" value="AAK42822"/>
        <property type="gene ID" value="SSO2711"/>
    </dbReference>
    <dbReference type="GeneID" id="1452741"/>
    <dbReference type="KEGG" id="sso:SSO2711"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2792"/>
    <dbReference type="eggNOG" id="arCOG02586">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1346461_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VC3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005358">
        <property type="entry name" value="Puta_zinc/iron-chelating_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03692">
        <property type="entry name" value="CxxCxxCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="24393" checksum="103B141EC93D8C24" modified="2001-10-01" version="1">MEINVDQINLMTKKGLRGDLKNFESVLDFLEKYGNATIVKYGMYSLVFQIAMNKFIDISKECEECGGKCCQIGYPVPVYGFDYEELRNKLSMEDLKKLEKVDSNLFLLRRPCKFQKGWYCSIHKFKPYACLSYPFATEDEQRDAINSYDGKGIPDFKVPEYCKAGMRVKHIMNQIITDLTNKLGRPPTPRELYNELKSRYYKNEETTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VD3</accession>
    <name>Q97VD3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TA0938 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2699</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90444">
        <property type="entry name" value="D90444"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988622.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VD3"/>
    <dbReference type="STRING" id="273057.SSO2699"/>
    <dbReference type="PaxDb" id="273057-SSO2699"/>
    <dbReference type="EnsemblBacteria" id="AAK42811">
        <property type="protein sequence ID" value="AAK42811"/>
        <property type="gene ID" value="SSO2699"/>
    </dbReference>
    <dbReference type="GeneID" id="1452730"/>
    <dbReference type="KEGG" id="sso:SSO2699"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2779"/>
    <dbReference type="eggNOG" id="arCOG06030">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2216919_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021585">
        <property type="entry name" value="Ta0938"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11494">
        <property type="entry name" value="Ta0938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="12025" checksum="CEA73BBC6C618A26" modified="2001-10-01" version="1">MKIIVNGKEAGTKEKGCALCGGTWGDYYEEIDGEKLFFCCDICALEFVNMVNEVKKRTNWSKIDELIINGNYYTGRTCSAKSGNKEYKFYVKFNDEAGIETFKELG</sequence>
</entry>
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    <accession>Q97VE7</accession>
    <name>Q97VE7_SACS2</name>
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        <recommendedName>
            <fullName evidence="7">Type II secretion system protein GspF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2679</name>
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    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Sulfolobales</taxon>
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            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="F90442"/>
    </dbReference>
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    <dbReference type="PaxDb" id="273057-SSO2679"/>
    <dbReference type="EnsemblBacteria" id="AAK42797">
        <property type="protein sequence ID" value="AAK42797"/>
        <property type="gene ID" value="SSO2679"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2679"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2761"/>
    <dbReference type="eggNOG" id="arCOG01808">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_414277_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VE7"/>
    <dbReference type="PhylomeDB" id="Q97VE7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="ArlJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
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        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402:SF1">
        <property type="entry name" value="TYPE II SECRETION SYSTEM PROTEIN GSPF DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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            <end position="37"/>
        </location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="167"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <begin position="548"/>
            <end position="577"/>
        </location>
    </feature>
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        <location>
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            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="633"/>
            <end position="657"/>
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    </feature>
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            <begin position="214"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="444"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="662" mass="75405" checksum="CC4B3D8A3D5D9EE1" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VF8</accession>
    <name>Q97VF8_SACS2</name>
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        <submittedName>
            <fullName evidence="7">ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="ordered locus">SSO2668</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="C90441"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988586.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="273057.SSO2668"/>
    <dbReference type="TCDB" id="3.A.1.5.9">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2668"/>
    <dbReference type="EnsemblBacteria" id="AAK42786">
        <property type="protein sequence ID" value="AAK42786"/>
        <property type="gene ID" value="SSO2668"/>
    </dbReference>
    <dbReference type="GeneID" id="1452705"/>
    <dbReference type="KEGG" id="sso:SSO2668"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2750"/>
    <dbReference type="eggNOG" id="arCOG00751">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_0_2"/>
    <dbReference type="InParanoid" id="Q97VF8"/>
    <dbReference type="PhylomeDB" id="Q97VF8"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="28"/>
        </location>
    </feature>
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        <location>
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            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="172"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="274"/>
        </location>
    </feature>
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        <location>
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            <end position="324"/>
        </location>
    </feature>
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        <location>
            <begin position="105"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="333" mass="37061" checksum="19F02C1C6C7E74B1" modified="2001-10-01" version="1">MNYKWLIRRLASVIIAIFVTIVISWALLEFSPYSPANYIMQFINPQDFAQKPELYASLIDYLNTLRPHGNPLINAVNYIWNTLHGNLGFSIISNVPVAELIAVALPWTLFIVVTSILISFFLGIRLGQKLGYLRGTKTDSVATVSLSILRSVPIYIYAVLLIYILAFIYHIFPTGGAYSIHVTPGFNLPFIASVLYHAFLPILTLTIVNLVGWILQMRANTIYVLGEDFVNFAEISGVKKDIIEKKYIGKNAILPLYTSLIISIGFSFSGSVFVEQTFSYPGVGNLLINSITSNDYPTEMGVFIIIIVAVIVGNLIADLTYSFLDPRAKVGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VH7</accession>
    <name>Q97VH7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transmembrane type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2646</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90438">
        <property type="entry name" value="H90438"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VH7"/>
    <dbReference type="STRING" id="273057.SSO2646"/>
    <dbReference type="PaxDb" id="273057-SSO2646"/>
    <dbReference type="DNASU" id="1452686"/>
    <dbReference type="EnsemblBacteria" id="AAK42767">
        <property type="protein sequence ID" value="AAK42767"/>
        <property type="gene ID" value="SSO2646"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2646"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2729"/>
    <dbReference type="eggNOG" id="arCOG01465">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089201_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VH7"/>
    <dbReference type="PhylomeDB" id="Q97VH7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051784">
        <property type="entry name" value="Nod_factor_ABC_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229:SF6">
        <property type="entry name" value="ABC-TYPE MULTIDRUG TRANSPORT SYSTEM, PERMEASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229">
        <property type="entry name" value="NODULATION PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="6">
        <location>
            <begin position="31"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="271" mass="30006" checksum="5B5DE9B7F19172E2" modified="2001-10-01" version="1">MGRKMGISGKLYSFLYLRGFKVWVSYRTQTVLTVLGWVLPVFTYYFTGTALGNRLVNEVGVSNYTAFFTIGLAFQGYVSSVISTISQRLRNEQLYGTIEYYVISRTGTFGFLLYSALWGLTLNTINAIIILAVGFALNIHYNVNIVSAIVIIVLLILSTLGIGMIAGAVTMVTKQGNPISFFFNTFTNLLGGTVFPVTVLPLYVRYISYGIPLTWALEGLREAFLNGAPITDIAPFIIILTIFDIVLLPLGVFSYNHAFKKAREKGTLGEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VS3</accession>
    <name>Q97VS3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-keto-4-pentenoate hydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2539</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90426">
        <property type="entry name" value="E90426"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990988.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VS3"/>
    <dbReference type="PaxDb" id="273057-SSO2539"/>
    <dbReference type="EnsemblBacteria" id="AAK42668">
        <property type="protein sequence ID" value="AAK42668"/>
        <property type="gene ID" value="SSO2539"/>
    </dbReference>
    <dbReference type="GeneID" id="1453995"/>
    <dbReference type="KEGG" id="sso:SSO2539"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2615"/>
    <dbReference type="eggNOG" id="arCOG06004">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369583_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VS3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="22562" checksum="2A259BCF6A820E5C" modified="2001-10-01" version="1">MKKAELLFDAYKSRKEIEPFPLAEDEAKKVKDEFVEMLINSEGLGGYKLSAFGEGVLTKPMITRENIVELWFRNHKLEVEIVALVKNGEVKRTFLGLEIPAPRFTTWDLPQHYIIADDIFAARLHVGPEIDPPFGSFKLYINDRFIGDGEPKYKPEEKVREYMEGYVSLGVFIGPISVKRRDKIRVEGKKSISVSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VV8</accession>
    <name>Q97VV8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl-CoA dehydrogenase related protein (Acd-like2)</fullName>
            <ecNumber evidence="3">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">acd-like2</name>
        <name evidence="3" type="ordered locus">SSO2497</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.3.99.-" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99422">
        <property type="entry name" value="A99422"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923900.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VV8"/>
    <dbReference type="STRING" id="273057.SSO2497"/>
    <dbReference type="PaxDb" id="273057-SSO2497"/>
    <dbReference type="EnsemblBacteria" id="AAK42632">
        <property type="protein sequence ID" value="AAK42632"/>
        <property type="gene ID" value="SSO2497"/>
    </dbReference>
    <dbReference type="GeneID" id="1453959"/>
    <dbReference type="KEGG" id="sso:SSO2497"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2577"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1044352_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VV8"/>
    <dbReference type="PhylomeDB" id="Q97VV8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016812">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="2">
        <location>
            <begin position="174"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="299" mass="33381" checksum="C7B6671C99CB4E16" modified="2001-10-01" version="1">MLLDLNFEISEDLKLILSSLNELLESRWSTKKLRAVMEGNKDYIEEIWKEIIKLDILPYLSTLSLRDNVIINEVIGRKLLPGIVVSSIVASRGIKNKDILNRVYAGEIRIAISDSNLVPSADEADYIIIGNRLIKRSNCTIRTFNSLDSSMKISKVDNIGNHEDIEVNNAEIALSLASQMVGSGEEVVSMSIKYSKERVAFGKPIGSYQAIKHRVVNDAIDVELARSLILEAAENIKYAWIAKDLANKKIPKAILSGIQVHGGIGFTDDLDIHLHLRRALTLSKLYNSKVNISEFLQPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W39</accession>
    <name>Q97W39_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90411">
        <property type="entry name" value="H90411"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989430.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W39"/>
    <dbReference type="PaxDb" id="273057-SSO2405"/>
    <dbReference type="EnsemblBacteria" id="AAK42551">
        <property type="protein sequence ID" value="AAK42551"/>
        <property type="gene ID" value="SSO2405"/>
    </dbReference>
    <dbReference type="GeneID" id="1453878"/>
    <dbReference type="KEGG" id="sso:SSO2405"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2486"/>
    <dbReference type="eggNOG" id="arCOG04165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151699_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W39"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="62"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="104" mass="12215" checksum="18CB0F9C56FB1391" modified="2001-10-01" version="1">MPTIHLSLPEWMYDELKQKADELGIQMTDLVKLFIKKGLEGDFEREEENEEKRTNAKYDESIAFLEAKVAQLDALLVEVLKKLEMLEEKDEEEEQVEVVDSNQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W74</accession>
    <name>Q97W74_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90407">
        <property type="entry name" value="C90407"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989486.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W74"/>
    <dbReference type="SMR" id="Q97W74"/>
    <dbReference type="STRING" id="273057.SSO2363"/>
    <dbReference type="PaxDb" id="273057-SSO2363"/>
    <dbReference type="EnsemblBacteria" id="AAK42514">
        <property type="protein sequence ID" value="AAK42514"/>
        <property type="gene ID" value="SSO2363"/>
    </dbReference>
    <dbReference type="GeneID" id="1453841"/>
    <dbReference type="KEGG" id="sso:SSO2363"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2448"/>
    <dbReference type="eggNOG" id="arCOG00434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018678_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W74"/>
    <dbReference type="PhylomeDB" id="Q97W74"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045427">
        <property type="entry name" value="MoxR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041538">
        <property type="entry name" value="RavA-like_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050513">
        <property type="entry name" value="RavA_ATPases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32204">
        <property type="entry name" value="ATPASE RAVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32204:SF0">
        <property type="entry name" value="ATPASE RAVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17868">
        <property type="entry name" value="AAA_lid_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20030">
        <property type="entry name" value="bpMoxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="37"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="382" mass="43684" checksum="A5EC988A55D19424" modified="2001-10-01" version="1">MSEKTLLELPKKFMEALMAPFIGREEEAKVITLALLSKEHVILIGEPGTAKSALARRAAELLNAKFFMYLLTKYTEPAELFGALDINALKDGQYKRITKDRLPESQIAFLDEIFNANSAILNALLSLLNERVIYDGYNVIKVPLRTLISASNRVPDEPELEALYDRLLLRHYARPVGEELWKQLLDATWEIEFTNRWAVKEPIMNIEHLDKLYSYLSQVDLSGVKNKLLKLYAMLEEKGIHLSDRRKGKVLKVVSAHAILNSRLKATEEDLIVLKYIAPREIDDFEKVAALLSEELKTPIKYMKELNEIYNNIKEAAKYVEAANESDPRLIELIRSLRATRDRIVALGKESGDEKVEEFSKEVLSEIDKLIEKVARKLGIYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W88</accession>
    <name>Q97W88_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90405">
        <property type="entry name" value="E90405"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989507.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W88"/>
    <dbReference type="STRING" id="273057.SSO2349"/>
    <dbReference type="PaxDb" id="273057-SSO2349"/>
    <dbReference type="EnsemblBacteria" id="AAK42500">
        <property type="protein sequence ID" value="AAK42500"/>
        <property type="gene ID" value="SSO2349"/>
    </dbReference>
    <dbReference type="GeneID" id="1453827"/>
    <dbReference type="KEGG" id="sso:SSO2349"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2433"/>
    <dbReference type="eggNOG" id="arCOG07197">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1598916_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W88"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="170" mass="18942" checksum="E5918FB05B7E09F0" modified="2001-10-01" version="1">MKASYTLPLSILMIILPVIPGLIDSFIGFLVGAIIDFVIAVYILISEKPWSNDIKTAISTLYFTTLSTFADVAGIFFAMAYQDEYKFAIVTLALSIPFIYNLFLVLKSILPNLIKADILDIGNGFFAFILVLIVGAIIGRVFITNFYALLPLYTGFLILAIIALFYFRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WA3</accession>
    <name>Q97WA3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CobQ/CobB/MinD/ParA nucleotide binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90403">
        <property type="entry name" value="F90403"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WA3"/>
    <dbReference type="SMR" id="Q97WA3"/>
    <dbReference type="STRING" id="273057.SSO2331"/>
    <dbReference type="PaxDb" id="273057-SSO2331"/>
    <dbReference type="EnsemblBacteria" id="AAK42485">
        <property type="protein sequence ID" value="AAK42485"/>
        <property type="gene ID" value="SSO2331"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2331"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2419"/>
    <dbReference type="eggNOG" id="arCOG07293">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1109540_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WA3"/>
    <dbReference type="PhylomeDB" id="Q97WA3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF10">
        <property type="entry name" value="ATPASE INVOLVED IN CHROMOSOME PARTITIONING, PARA_MIND FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="1">
        <location>
            <begin position="17"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="256" mass="29073" checksum="0077B6438D030E98" modified="2001-10-01" version="1">MQWRRVLRFVNMEVVYVLGVKGGIGKTTFSLYFANQLSLTGKKVLYIDHDYFSFGSLILGHNDLGLLEEIENNLPIFSRSLKNINNLYILKLFSNPLDANKDYSLLSNSIPQVVNSIAKMGLDYVILDSSVGILPDNDIVIGLLEELDVEKKGVFLSDMLSVNSTIKYAKLWENAIKYKILVINMVPPIPEDLSEAMKIANKVYDDNNSKLFNTVVVVPFDEKMYNYKPLGNEYENERLNTILSCIVNRCNTLIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WA4</accession>
    <name>Q97WA4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1058</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90403">
        <property type="entry name" value="E90403"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WA4"/>
    <dbReference type="STRING" id="273057.SSO2329"/>
    <dbReference type="PaxDb" id="273057-SSO2329"/>
    <dbReference type="EnsemblBacteria" id="AAK42484">
        <property type="protein sequence ID" value="AAK42484"/>
        <property type="gene ID" value="SSO2329"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2329"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2418"/>
    <dbReference type="eggNOG" id="arCOG02754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1551894_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WA4"/>
    <dbReference type="PhylomeDB" id="Q97WA4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053172">
        <property type="entry name" value="Tn903_transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631:SF3">
        <property type="entry name" value="ISSOD12 TRANSPOSASE TNPA_ISSOD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="13"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="168" mass="19316" checksum="5BA732DA591474F9" modified="2001-10-01" version="1">MRNMNITFPETSDELEVIADATGISTNTGGQYIVAKWGKTRDSKFLKIEIVMDNNEFNVVNAEVTSNEVESVKTVKDLQDKGKKFYGDKTYDVNEVYKTGVEVVVPPKKNASTKRHLARRKAVREFRKLGYDRWREEKGYGVRWRVESLFSAVRRTFGESVRQVLLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WE7</accession>
    <name>Q97WE7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MutT-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2278</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90398">
        <property type="entry name" value="B90398"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WE7"/>
    <dbReference type="STRING" id="273057.SSO2278"/>
    <dbReference type="PaxDb" id="273057-SSO2278"/>
    <dbReference type="EnsemblBacteria" id="AAK42441">
        <property type="protein sequence ID" value="AAK42441"/>
        <property type="gene ID" value="SSO2278"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2278"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2372"/>
    <dbReference type="eggNOG" id="arCOG01075">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_20_2_2"/>
    <dbReference type="InParanoid" id="Q97WE7"/>
    <dbReference type="PhylomeDB" id="Q97WE7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04673">
        <property type="entry name" value="NUDIX_ADPRase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43046">
        <property type="entry name" value="GDP-MANNOSE MANNOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43046:SF14">
        <property type="entry name" value="MUTT_NUDIX FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="26"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="164" mass="18739" checksum="B54C83829C15145E" modified="2001-10-01" version="1">MILLLISYTIHHKSLNINFYHYFMDRPLVAVGCLIVEENKVLLVQRKNPPNAGLWAIPGGKVEYGETLEEALKREMREETGLEVAVGNIISIVQVINEGFHYVILDFECKPIGGNLRASTDAVKVEYVPFDKLNIIQTTKTTYDMLSMYFRGEKPPYFIIQISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WF3</accession>
    <name>Q97WF3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Iron dependent repressor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2273</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2010" name="J. Struct. Funct. Genomics" volume="11" first="167" last="180">
            <title>The Scottish Structural Proteomics Facility: targets, methods and outputs.</title>
            <authorList>
                <person name="Oke M."/>
                <person name="Carter L.G."/>
                <person name="Johnson K.A."/>
                <person name="Liu H."/>
                <person name="McMahon S.A."/>
                <person name="Yan X."/>
                <person name="Kerou M."/>
                <person name="Weikart N.D."/>
                <person name="Kadi N."/>
                <person name="Sheikh M.A."/>
                <person name="Schmelz S."/>
                <person name="Dorward M."/>
                <person name="Zawadzki M."/>
                <person name="Cozens C."/>
                <person name="Falconer H."/>
                <person name="Powers H."/>
                <person name="Overton I.M."/>
                <person name="van Niekerk C.A."/>
                <person name="Peng X."/>
                <person name="Patel P."/>
                <person name="Garrett R.A."/>
                <person name="Prangishvili D."/>
                <person name="Botting C.H."/>
                <person name="Coote P.J."/>
                <person name="Dryden D.T."/>
                <person name="Barton G.J."/>
                <person name="Schwarz-Linek U."/>
                <person name="Challis G.L."/>
                <person name="Taylor G.L."/>
                <person name="White M.F."/>
                <person name="Naismith J.H."/>
            </authorList>
            <dbReference type="PubMed" id="20419351"/>
            <dbReference type="DOI" id="10.1007/s10969-010-9090-y"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) IN COMPLEX WITH ZN(2+)</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtxR/MntR family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90397">
        <property type="entry name" value="D90397"/>
    </dbReference>
    <dbReference type="PDB" id="2X4H">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B/C/D=1-138"/>
    </dbReference>
    <dbReference type="PDBsum" id="2X4H"/>
    <dbReference type="AlphaFoldDB" id="Q97WF3"/>
    <dbReference type="SMR" id="Q97WF3"/>
    <dbReference type="STRING" id="273057.SSO2273"/>
    <dbReference type="PaxDb" id="273057-SSO2273"/>
    <dbReference type="EnsemblBacteria" id="AAK42435">
        <property type="protein sequence ID" value="AAK42435"/>
        <property type="gene ID" value="SSO2273"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2273"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2365"/>
    <dbReference type="eggNOG" id="arCOG02100">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069532_4_1_2"/>
    <dbReference type="InParanoid" id="Q97WF3"/>
    <dbReference type="PhylomeDB" id="Q97WF3"/>
    <dbReference type="EvolutionaryTrace" id="Q97WF3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="11"/>
            <end position="65"/>
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            <begin position="70"/>
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            <position position="14"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02742"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAK42435.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
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    </evidence>
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        <source>
            <dbReference type="PDB" id="2X4H"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15878" checksum="764ABC2D324E39A8" modified="2001-10-01" version="1">MRVDSQMSNLSRREFSYLLTIKRYNDSGEGAKINRIAKDLKIAPSSVFEEVSHLEEKGLVKKKEDGVWITNNGTRSINYLIKAHRVIEILLVNIGIDKQTACEYSKQFDYLIPEEIIDKLYNYLGKPSYCPHGLEIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WF7</accession>
    <name>Q97WF7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methylmalonyl-CoA mutase, alpha-subunit, chain B (McmA2)</fullName>
            <ecNumber evidence="7">5.4.99.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mcmA2</name>
        <name evidence="7" type="ordered locus">SSO2266</name>
    </gene>
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        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="G90396"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    </dbReference>
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    <dbReference type="PaxDb" id="273057-SSO2266"/>
    <dbReference type="EnsemblBacteria" id="AAK42430">
        <property type="protein sequence ID" value="AAK42430"/>
        <property type="gene ID" value="SSO2266"/>
    </dbReference>
    <dbReference type="GeneID" id="1453757"/>
    <dbReference type="KEGG" id="sso:SSO2266"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2357"/>
    <dbReference type="eggNOG" id="arCOG01710">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_128233_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WF7"/>
    <dbReference type="PhylomeDB" id="Q97WF7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004494">
        <property type="term" value="F:methylmalonyl-CoA mutase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="MM_CoA_mut_B12_BD"/>
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        <property type="entry name" value="Cobalamin-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="Acid_CoA_mut_C"/>
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        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
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        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00640">
        <property type="entry name" value="acid_CoA_mut_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101:SF3">
        <property type="entry name" value="COENZYME B12-DEPENDENT MUTASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="B12_BINDING"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="5" id="KW-0170">Cobalt</keyword>
    <keyword evidence="4 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="6">
        <location>
            <begin position="7"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="141" mass="15259" checksum="1C2019309AA73832" modified="2001-10-01" version="1">MMITTKRIKVIVAKLGLDGHDRGAKVVARALKDAGMEVVYTGLRQTPEQIVRAALQEDADVIGISILSGAHLELIPKVVEIMKQNGLNDVGLIVGGVIPPEDIKKLKEMGVDEVFLPGSSLKEIVEKVKKVAREKRGISVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WG5</accession>
    <name>Q97WG5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="2">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">bcp-3</name>
        <name evidence="13" type="ordered locus">SSO2255</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peroxiredoxin family. BCP/PrxQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90395">
        <property type="entry name" value="G90395"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991535.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WG5"/>
    <dbReference type="FunCoup" id="Q97WG5">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2255"/>
    <dbReference type="PaxDb" id="273057-SSO2255"/>
    <dbReference type="EnsemblBacteria" id="AAK42422">
        <property type="protein sequence ID" value="AAK42422"/>
        <property type="gene ID" value="SSO2255"/>
    </dbReference>
    <dbReference type="GeneID" id="1453749"/>
    <dbReference type="KEGG" id="sso:SSO2255"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2349"/>
    <dbReference type="eggNOG" id="arCOG00310">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_14_2_2"/>
    <dbReference type="InParanoid" id="Q97WG5"/>
    <dbReference type="PhylomeDB" id="Q97WG5"/>
    <dbReference type="BRENDA" id="1.11.1.24">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140824">
        <property type="term" value="F:thioredoxin-dependent peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03017">
        <property type="entry name" value="PRX_BCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000007">
        <property type="entry name" value="Thioredoxin-dependent thiol peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050924">
        <property type="entry name" value="Peroxiredoxin_BCP/PrxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801:SF4">
        <property type="entry name" value="AHPC_TSA FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="12">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="11">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAK42422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="149" mass="16976" checksum="6FD4EB625F93B8EA" modified="2001-10-01" version="1">MNVGEEAPDFEAESTLGKIKLSDFRGKKVILYFYPKSFTSGCTRELQRFTELYDEFKKLNAEVIGVSVDKIDTQKKFAEKYGAKFPIVADDQKTISKLYSVLNERGTSAQRVTFIIDENGKIVNILKNLKKAEEHADKSLEIIKRQNST</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2015-10-14" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WG8</accession>
    <name>HERA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA double-strand break repair helicase HerA</fullName>
            <ecNumber evidence="2 3">5.6.2.3</ecNumber>
            <ecNumber evidence="2">5.6.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Bidirectional DNA 3'-5' and 5'-3' helicase HerA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">herA</name>
        <name evidence="8" type="ordered locus">SSO2251</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="Nucleic Acids Res." volume="40" first="3183" last="3196">
            <title>Structural and functional insights into DNA-end processing by the archaeal HerA helicase-NurA nuclease complex.</title>
            <authorList>
                <person name="Blackwood J.K."/>
                <person name="Rzechorzek N.J."/>
                <person name="Abrams A.S."/>
                <person name="Maman J.D."/>
                <person name="Pellegrini L."/>
                <person name="Robinson N.P."/>
            </authorList>
            <dbReference type="PubMed" id="22135300"/>
            <dbReference type="DOI" id="10.1093/nar/gkr1157"/>
        </citation>
        <scope>FUNCTION AS A BIDIRECTIONAL HELICASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH NURA</scope>
        <scope>MUTAGENESIS OF LYS-154</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2014" name="FEBS Lett." volume="588" first="4637" last="4644">
            <title>Molecular architecture of the HerA-NurA DNA double-strand break resection complex.</title>
            <authorList>
                <person name="Byrne R.T."/>
                <person name="Schuller J.M."/>
                <person name="Unverdorben P."/>
                <person name="Foerster F."/>
                <person name="Hopfner K.P."/>
            </authorList>
            <dbReference type="PubMed" id="25447518"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2014.10.035"/>
        </citation>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH NURA</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2015" name="PLoS ONE" volume="10" first="e0142345" last="e0142345">
            <title>NurA Is Endowed with Endo- and Exonuclease Activities that Are Modulated by HerA: New Insight into Their Role in DNA-End Processing.</title>
            <authorList>
                <person name="De Falco M."/>
                <person name="Catalano F."/>
                <person name="Rossi M."/>
                <person name="Ciaramella M."/>
                <person name="De Felice M."/>
            </authorList>
            <dbReference type="PubMed" id="26560692"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0142345"/>
        </citation>
        <scope>FUNCTION AS A HELICASE</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <scope>DNA-BINDING</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2018" name="Extremophiles" volume="22" first="581" last="589">
            <title>The Sulfolobus solfataricus RecQ-like DNA helicase Hel112 inhibits the NurA/HerA complex exonuclease activity.</title>
            <authorList>
                <person name="De Falco M."/>
                <person name="Massa F."/>
                <person name="Rossi M."/>
                <person name="De Felice M."/>
            </authorList>
            <dbReference type="PubMed" id="29488113"/>
            <dbReference type="DOI" id="10.1007/s00792-018-1018-7"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="9" key="6">
        <citation type="journal article" date="2014" name="Nat. Commun." volume="5" first="5506" last="5506">
            <title>Structure of the hexameric HerA ATPase reveals a mechanism of translocation-coupled DNA-end processing in archaea.</title>
            <authorList>
                <person name="Rzechorzek N.J."/>
                <person name="Blackwood J.K."/>
                <person name="Bray S.M."/>
                <person name="Maman J.D."/>
                <person name="Pellegrini L."/>
                <person name="Robinson N.P."/>
            </authorList>
            <dbReference type="PubMed" id="25420454"/>
            <dbReference type="DOI" id="10.1038/NCOMMS6506"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.84 ANGSTROMS) IN COMPLEX WITH ATP ANALOG</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
        <scope>DOMAIN</scope>
        <scope>MUTAGENESIS OF ARG-142; LYS-154; ARG-381 AND ASP-471</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 3 5">Involved in DNA double-strand break (DSB) repair (PubMed:22135300). Probably acts with NurA to stimulate resection of the 5' strand and produce the long 3' single-strand that is required for RadA loading (By similarity). NurA and HerA together stimulate the end-resection of six nucleotides of a linear DNA substrate (PubMed:26560692). Has DNA-dependent ATPase activity and bidirectional DNA helicase activity (PubMed:22135300, PubMed:25420454, PubMed:26560692). Preferentially binds single stranded (ss)DNA, bubble and semiforked DNA substrate over other DNA molecules tested (PubMed:26560692). Stimulates the exo- but not endonuclease activity of NurA (PubMed:26560692).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3 5">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA at the replication fork by translocating in the 5'-3' direction.</text>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="2 5">ATPase activity is stimulated in the presence of linear double-stranded (ds)DNA (PubMed:22135300). Helicase activity requires the presence of NurA (PubMed:22135300, PubMed:26560692). LhrC-Core (Hel112) inhibits the exonuclease activity of the HerA-NurA complex on ss- and dsDNA, has no effect on the nicking activity of NurA (PubMed:29488113).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3 4 5">Homohexamer (PubMed:22135300, PubMed:25420454, PubMed:25447518, PubMed:26560692). Forms a complex with NurA (PubMed:22135300, PubMed:25447518, PubMed:26560692).</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-10110451">
            <id>Q97WG8</id>
        </interactant>
        <interactant intactId="EBI-10110462">
            <id>Q97WH1</id>
            <label>nurA</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>3</experiments>
    </comment>
    <comment type="domain">
        <text evidence="3">Contains an N-terminal HAS-barrel domain, which mediates interaction with NurA, followed by a central RecA-like catalytic core and a C-terminal four-helix bundle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the HerA family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="2 3"/>
    <dbReference type="EC" id="5.6.2.4" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90395">
        <property type="entry name" value="D90395"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991539.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="4D2I">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.84 A"/>
        <property type="chains" value="A/B=1-500"/>
    </dbReference>
    <dbReference type="PDBsum" id="4D2I"/>
    <dbReference type="AlphaFoldDB" id="Q97WG8"/>
    <dbReference type="EMDB" id="EMD-2808"/>
    <dbReference type="SMR" id="Q97WG8"/>
    <dbReference type="IntAct" id="Q97WG8">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="MINT" id="Q97WG8"/>
    <dbReference type="STRING" id="273057.SSO2251"/>
    <dbReference type="PaxDb" id="273057-SSO2251"/>
    <dbReference type="EnsemblBacteria" id="AAK42419">
        <property type="protein sequence ID" value="AAK42419"/>
        <property type="gene ID" value="SSO2251"/>
    </dbReference>
    <dbReference type="GeneID" id="1453746"/>
    <dbReference type="GeneID" id="44127985"/>
    <dbReference type="KEGG" id="sso:SSO2251"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2346"/>
    <dbReference type="eggNOG" id="arCOG00280">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023842_2_0_2"/>
    <dbReference type="InParanoid" id="Q97WG8"/>
    <dbReference type="PhylomeDB" id="Q97WG8"/>
    <dbReference type="EvolutionaryTrace" id="Q97WG8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="DSB_Repair_Helicase"/>
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        <property type="entry name" value="HerA-like"/>
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        <property type="entry name" value="HerA_barrel_dom"/>
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        <property type="entry name" value="HerA_central"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="NCBIfam" id="NF040937">
        <property type="entry name" value="HerA_Thermprot"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HAS-barrel"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
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    <keyword id="KW-0234">DNA repair</keyword>
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    <keyword id="KW-0413">Isomerase</keyword>
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    <keyword id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="500"/>
        </location>
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    <feature type="binding site" evidence="3 9">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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            <begin position="151"/>
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        </location>
        <ligand>
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            <begin position="459"/>
            <end position="460"/>
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        <ligand>
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        <variation>A</variation>
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            <position position="142"/>
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        <original>K</original>
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        </location>
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    <sequence length="500" mass="56329" checksum="6CCFAA3F2DB23879" modified="2001-10-01" version="1">MIIGYVIGQATTQEALILAERPVRLGTYVVLEYDNVKALGLITNVTRGSPMLDDNMNDIEIVQRLKQFNNSIPVYTKAKVKMLCDMNNHFLMPDIPPFAGTPAREAEDEELKSIYSQDGQIRIGSLIGKNVEVKLNINSFARHLAILAATGSGKSNTVAVLSQRISELGGSVLIFDYHGEYYDSDIKNLNRIEPKLNPLYMTPREFSTLLEIRENAIIQYRILRRAFIKVTNGIREKLKEGQIPFSTLNSQFYELMKDELETQGNSDKKSSAKDEVLNKFEEFMDRYSNVIDLTSSDIIEKVKRGKVNVVSLTQLDEDSMDAVVSHYLRRILDSRKDFKRSKNSGLKFPIIAVIEEAHVFLSKNENTLTKYWASRIAREGRKFGVGLTIVSQRPKGLDENILSQMTNKIILKIIEPTDKKYILESSDNLSEDLAEQLSSLDVGEAIIIGKIVKLPAVVKIDMFEGKLLGSDPDMIGEWKKVEESEKIAKGFADFGTEIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WI5</accession>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
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            <dbReference type="PubMed" id="11427726"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <dbReference type="GeneID" id="1453686"/>
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        <property type="entry name" value="MSC_TM_helix"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05552">
        <property type="entry name" value="MS_channel_1st_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="281" mass="30271" checksum="2E355144BE493B7F" modified="2001-10-01" version="1">MNIQRISVIIVSAQTSISQALSGIATSIIDAIPSIILFVIILLVGYIIGNIVAYSIRTFLGRIFREEHVRASVDIIAGTVKALIILIALSIALSFLQLGAASGYIQQIANYLPKLAGAIVLLTIGLTLVNILVDYMQRQIGARSQDDLITAIFNVLRFGLYAAIITVAAALAIFSVIPYVDPYVFYAVILGAVILYAAYALIDKVLVPFANSNPDLAYVANYGRFILYIIVLLIAIAIIVEPFGNVTSIIYALSWGLAIAFAIALIPLVIALVKKFLAEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WN3</accession>
    <name>Q97WN3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">malonyl CoA reductase (malonate semialdehyde-forming)</fullName>
            <ecNumber evidence="5">1.2.1.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">asd-2</name>
        <name evidence="8" type="ordered locus">SSO2178</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>3-oxopropanoate + NADP(+) + CoA = malonyl-CoA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26446"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33190"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.75"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.75" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90387">
        <property type="entry name" value="B90387"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992122.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WN3"/>
    <dbReference type="FunCoup" id="Q97WN3">
        <property type="interactions" value="242"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2178"/>
    <dbReference type="PaxDb" id="273057-SSO2178"/>
    <dbReference type="EnsemblBacteria" id="AAK42353">
        <property type="protein sequence ID" value="AAK42353"/>
        <property type="gene ID" value="SSO2178"/>
    </dbReference>
    <dbReference type="GeneID" id="1453680"/>
    <dbReference type="KEGG" id="sso:SSO2178"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2274"/>
    <dbReference type="eggNOG" id="arCOG00494">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049966_1_0_2"/>
    <dbReference type="InParanoid" id="Q97WN3"/>
    <dbReference type="PhylomeDB" id="Q97WN3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd24150">
        <property type="entry name" value="ASADH_MCR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000075">
        <property type="entry name" value="Aspartate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000411">
        <property type="entry name" value="Aspartate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051823">
        <property type="entry name" value="ASADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005676">
        <property type="entry name" value="Asp_semi-ald_DH_pep-lack"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00978">
        <property type="entry name" value="asd_EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006416">
        <property type="entry name" value="PRK08664.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718:SF1">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000148">
        <property type="entry name" value="ASA_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="7">
        <location>
            <begin position="6"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="6">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00052213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000148-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="356" mass="39161" checksum="9BAB970F6B14E85B" modified="2001-10-01" version="1">MRRTLKAAILGSTGLVGIEYVRMLANHPYIKVGYLAGKGSVGKPYGEVVRWQTIGQVPTEIADMEVKPTDPKLMDDVDIVFSPLPQGAAGPVEEEFAKHGFPVISNSPDHRFDPDVPLLIPEVNPHTISLIDKQRERRDWRGFIVTTPLCTAQGAAIPLAPIYMNFKIDSSLITTIQSLSGAGYPGIPSLDVIDNVLPLGDNYDNKTVKEISRILSETKRMVDDDDNNLSLGATTHRVATIHGHYEVIYITFKEDVTVEKIGETLDSFRGEPQNLKLPTAPDKPILLINQDARPQVYFDRWAGNPPGMSVVVGRLGQINRRTIRLVSVIHNTVRGAAGGGILATELLIEKGYIDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WP6</accession>
    <name>Q97WP6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO9761</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK67525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WP6"/>
    <dbReference type="STRING" id="273057.SSO9761"/>
    <dbReference type="PaxDb" id="273057-SSO9761"/>
    <dbReference type="EnsemblBacteria" id="AAK67525">
        <property type="protein sequence ID" value="AAK67525"/>
        <property type="gene ID" value="SSO9761"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO9761"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2911714_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WP6"/>
    <dbReference type="PhylomeDB" id="Q97WP6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025471">
        <property type="entry name" value="DUF4322"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14210">
        <property type="entry name" value="DUF4322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4322" evidence="1">
        <location>
            <begin position="7"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK67525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="61" mass="6973" checksum="C6AA85CEA3373469" modified="2001-10-01" version="1">MPIPSLNIQQIVYKLLSMINFHGRKGEEATKTSVSASLHNDSVEKRFQSLQRITTNSEELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WR4</accession>
    <name>Q97WR4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">4-hydroxyphenylacetate-3-hydroxylase (HpaA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hpaA</name>
        <name evidence="6" type="ordered locus">SSO2053</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90372">
        <property type="entry name" value="G90372"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WR4"/>
    <dbReference type="FunCoup" id="Q97WR4">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2053"/>
    <dbReference type="PaxDb" id="273057-SSO2053"/>
    <dbReference type="EnsemblBacteria" id="AAK42238">
        <property type="protein sequence ID" value="AAK42238"/>
        <property type="gene ID" value="SSO2053"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2053"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2133"/>
    <dbReference type="eggNOG" id="arCOG02143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023920_2_1_2"/>
    <dbReference type="InParanoid" id="Q97WR4"/>
    <dbReference type="PhylomeDB" id="Q97WR4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3140.10">
        <property type="entry name" value="4-hydroxybutyryl-coa dehydratase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004925">
        <property type="entry name" value="HpaB/PvcC/4-BUDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024719">
        <property type="entry name" value="HpaB/PvcC/4-BUDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024674">
        <property type="entry name" value="HpaB/PvcC/4-BUDH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36117">
        <property type="entry name" value="4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36117:SF3">
        <property type="entry name" value="4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03241">
        <property type="entry name" value="HpaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11794">
        <property type="entry name" value="HpaB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000331">
        <property type="entry name" value="HpaA_HpaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpaB/PvcC/4-BUDH N-terminal" evidence="5">
        <location>
            <begin position="22"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="HpaB/PvcC/4-BUDH C-terminal" evidence="4">
        <location>
            <begin position="301"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="508" mass="59046" checksum="9960EF858BCE316B" modified="2001-10-01" version="1">MGCRGGYFHGSIKGVVWMIRRGTDYIKSIKENPPVVYYEGEVVKDVTEHSAFKIPISTVAKYYDLHWDEKYKEYLRVYNLDVGEETSISFLRPKNKKDLGKLRDGLVKIYDYYRGFFGRSPDYLNLWTMVFYAHAEDYFGKQFGSKFMENAIEIYKEATKRDLFYTHAIVAPMYDRSRPPSQWEDPYIQVGIVEEKPEGVVVRGAAMICTAGPYAEMLWYLPNVRRDSDPRYAIYFSIPTNTKGVKFLSRRGFQPREGGEFEYPISSRFEESDAVLVLDNVLIPWERIIFFKKPELIEDLMWHTVQLRGWFNWHFVIQHYSRLKFLAGLAIAIAEASGTSNFINVQEKIGEILIYVALNEAALYASVERAQELPNITRPDPYISISASHFNMKTVPRANEILRSISAGSSIPIPAGIKDFENPEERKLLDKYMAMKGLDALERVKMFNLLWDVIGSEAGMRYEQYDRFSRGDPTIRWAQTYTEVFKDRKHEFVKLVKDIIDQMPNPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WS5</accession>
    <name>Q97WS5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acetyl-CoA synthetase (Acetate-CoA ligase) (AcsA-5)</fullName>
            <ecNumber evidence="8">6.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">acsA-5</name>
        <name evidence="8" type="ordered locus">SSO2041</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90371">
        <property type="entry name" value="D90371"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923747.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WS5"/>
    <dbReference type="FunCoup" id="Q97WS5">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2041"/>
    <dbReference type="PaxDb" id="273057-SSO2041"/>
    <dbReference type="EnsemblBacteria" id="AAK42227">
        <property type="protein sequence ID" value="AAK42227"/>
        <property type="gene ID" value="SSO2041"/>
    </dbReference>
    <dbReference type="GeneID" id="1453554"/>
    <dbReference type="KEGG" id="sso:SSO2041"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2122"/>
    <dbReference type="eggNOG" id="arCOG04201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_10_2"/>
    <dbReference type="InParanoid" id="Q97WS5"/>
    <dbReference type="PhylomeDB" id="Q97WS5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015645">
        <property type="term" value="F:fatty acid ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000097">
        <property type="entry name" value="AMP-dependent synthetase and ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051087">
        <property type="entry name" value="Mitochondrial_ACSM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605:SF10">
        <property type="entry name" value="ACYL-COA SYNTHETASE MEDIUM CHAIN FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605">
        <property type="entry name" value="ACYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="6">
        <location>
            <begin position="42"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="7">
        <location>
            <begin position="411"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="498" mass="57092" checksum="A6D57E5C1FAE3B4B" modified="2001-10-01" version="1">MASYEEVRRSFSWSEVIRYLDENPLDVLTSHDGIAVKRFTKNAMEEISFSDLKRKALRLALYLKEFHNIKKGDVIAILASKKIQQIIVFLATLSLGAIYQPLFTAFGPEAIKMRTRDVKPKIIFCQDDQKDKINDAILFSKFDELLSYGELKEIEKINWDDAIILLYTSGTTGTPKGALIAKRLFLNIYVYMKYGIGVRENDVFWNPADPGWAYGLYYGIIGPLMFGKTIIFLDEPFNPERTMEFMEENKVTNFAFAPTAYRMIAGTVKKKYDLVLERASSAGEPLNPEVIRWFMDKYNVTIKDHYGQTEVGMVVYNGWGYDAKVKIGSMGLPAPGYEVDIIEDIIAVKRDSPGFHFLGYLNNPEKTKESFRGDWYLTGDNAYKDEEGYLWFVGRKDDVVKVSGYRIGPFEVESVLLEFPAVLESAVVADEDPIRGHVLHAYIVLKSGYTPSEELKKEIIDFVNTKYSRHVHLERVDFVDKLPKTESGKIQRYLLRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-11-27" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X85</accession>
    <name>Q97X85_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hemerythrin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1869</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90350">
        <property type="entry name" value="B90350"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X85"/>
    <dbReference type="SMR" id="Q97X85"/>
    <dbReference type="STRING" id="273057.SSO1869"/>
    <dbReference type="PaxDb" id="273057-SSO1869"/>
    <dbReference type="EnsemblBacteria" id="AAK42057">
        <property type="protein sequence ID" value="AAK42057"/>
        <property type="gene ID" value="SSO1869"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1869"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1920"/>
    <dbReference type="eggNOG" id="arCOG01471">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095978_2_0_2"/>
    <dbReference type="InParanoid" id="Q97X85"/>
    <dbReference type="PhylomeDB" id="Q97X85"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.520">
        <property type="entry name" value="nmb1532 protein domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39966">
        <property type="entry name" value="BLL2471 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39966:SF1">
        <property type="entry name" value="HEMERYTHRIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="176" mass="21030" checksum="6E256A0829720C28" modified="2001-10-01" version="1">MMLNSIKTLMEEHTVILKALEILNVIDINENNMQDIKIIIDFIKNFVDNCHHVKEEKVLFDFLEHKGMVGGPIYVMVYEHNKLRDIINRIEARYNEFEELREDLNSLIVLLASHIDKENNVLFPTAESLLSDDENKVIYEKFERIEEEFGKERHERYIELIDTLYGRYINKKSNER</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XA8</accession>
    <name>Q97XA8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1838</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90347">
        <property type="entry name" value="C90347"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992717.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XA8"/>
    <dbReference type="STRING" id="273057.SSO1838"/>
    <dbReference type="PaxDb" id="273057-SSO1838"/>
    <dbReference type="EnsemblBacteria" id="AAK42034">
        <property type="protein sequence ID" value="AAK42034"/>
        <property type="gene ID" value="SSO1838"/>
    </dbReference>
    <dbReference type="GeneID" id="1453361"/>
    <dbReference type="KEGG" id="sso:SSO1838"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1898"/>
    <dbReference type="eggNOG" id="arCOG05401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130233_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XA8"/>
    <dbReference type="PhylomeDB" id="Q97XA8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="162" mass="18075" checksum="A000C6CC5F8F82E9" modified="2001-10-01" version="1">MSQENNENNKKTITIRGVDEKIYRKLTEIARSSGKTIGEVTNQAFKTFLDISDAAKRTASGVVESGKTFIEGFKEGMGNLIIISDIDELSVNKEEIMQTSKPIAFKGIKKLSLVGISDDDIEKYIQEISDVDELIIPSSVNKLKLLQKCRRVKRVVVQNNPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XC0</accession>
    <name>Q97XC0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1824</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90345">
        <property type="entry name" value="F90345"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923611.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XC0"/>
    <dbReference type="FunCoup" id="Q97XC0">
        <property type="interactions" value="162"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1824"/>
    <dbReference type="EnsemblBacteria" id="AAK42021">
        <property type="protein sequence ID" value="AAK42021"/>
        <property type="gene ID" value="SSO1824"/>
    </dbReference>
    <dbReference type="GeneID" id="1454800"/>
    <dbReference type="KEGG" id="sso:SSO1824"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1884"/>
    <dbReference type="eggNOG" id="arCOG04430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026821_3_0_2"/>
    <dbReference type="InParanoid" id="Q97XC0"/>
    <dbReference type="PhylomeDB" id="Q97XC0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
        <property type="entry name" value="HD_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="48"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="420" mass="49215" checksum="BB6EBEF31B57BA94" modified="2001-10-01" version="1">MEKYIKDPIYGNIKVESKLIDNPYFQRLKHIIQNGMAYMIFPSMRHTRFEHSLGTYYIASKMIDRIEGNIDSDFKEIIKKLALYHDIGHFPFSHTFEFALDVLKYLNDDKYKEIIGLTEYKGQLHKFHEMMGIKILKDGLGEKEVADLMENVYTSSNPLDDKIKIAKLIVNSDLDADRLDYLQRDSYYSGAKFGIIDPERIIITMTITPKGYIFPPKAIDDLEHFFLARFHMYSSVYNHPVIEIYNRVMSYFIAFAIHKGLLKIPSNLEEIKTFTDEQVFLALINAKNDNELSHFYFSLVERRKYKRAVLQGSEARTLHKYLTEDEDAKEFYEYILGYKGKVVIGSTELKTSIDNIQIKEKLGKYEYLNNLKEESSVVKPPERFRVHIGVYDDNVAKEISEYFSKNFSITLDFRNLESNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XG1</accession>
    <name>Q97XG1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">dTDP-Glucose 4,6-dehydratase (RfbB-3)</fullName>
            <ecNumber evidence="3">4.2.1.46</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rfbB-3</name>
        <name evidence="3" type="ordered locus">SSO1781</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.46" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90339">
        <property type="entry name" value="F90339"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990175.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XG1"/>
    <dbReference type="FunCoup" id="Q97XG1">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1781"/>
    <dbReference type="PaxDb" id="273057-SSO1781"/>
    <dbReference type="EnsemblBacteria" id="AAK41973">
        <property type="protein sequence ID" value="AAK41973"/>
        <property type="gene ID" value="SSO1781"/>
    </dbReference>
    <dbReference type="GeneID" id="1454752"/>
    <dbReference type="KEGG" id="sso:SSO1781"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1831"/>
    <dbReference type="eggNOG" id="arCOG01371">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_2"/>
    <dbReference type="InParanoid" id="Q97XG1"/>
    <dbReference type="PhylomeDB" id="Q97XG1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="312" mass="36210" checksum="2F33A5FEA7318A4C" modified="2001-10-01" version="1">MIIIGGAGFIGSAFVREVNKRGIKPIVVDLLTYAGRKENLIGTEHEFIRADVRSEEIHDIVKNSDIVVNFAAETHVDRSIYRPQDFVTTNVLGVVNLLEAARKYDFKYVHISTDEVYGEECADEDSPLQPSSPYSASKASADLFVKAYVRTYGIKAIIVRPSNNYGPRQFPEKLIPKVIIRTFLNMHVPIYGDGRAERDWIYVEDTVRIIYDIMERSEWRGEVYNIPGGQRYSVLDVIKIIGEIMGKEVKVKFVDDRPGHDKRYCMTTKLKYEVTPLKEGLRKTVEWYLNNRWWWEDLIKDKFFTEDEPWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XG4</accession>
    <name>Q97XG4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase FkbM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1777</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90339">
        <property type="entry name" value="C90339"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988671.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XG4"/>
    <dbReference type="STRING" id="273057.SSO1777"/>
    <dbReference type="PaxDb" id="273057-SSO1777"/>
    <dbReference type="EnsemblBacteria" id="AAK41970">
        <property type="protein sequence ID" value="AAK41970"/>
        <property type="gene ID" value="SSO1777"/>
    </dbReference>
    <dbReference type="GeneID" id="1454749"/>
    <dbReference type="KEGG" id="sso:SSO1777"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1827"/>
    <dbReference type="eggNOG" id="arCOG01400">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1052190_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XG4"/>
    <dbReference type="PhylomeDB" id="Q97XG4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052514">
        <property type="entry name" value="SAM-dependent_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203">
        <property type="entry name" value="METHYLTRANSFERASE, FKBM FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203:SF15">
        <property type="entry name" value="SLL1173 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase FkbM" evidence="1">
        <location>
            <begin position="86"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="264" mass="30561" checksum="80A8DF705FDA17AD" modified="2001-10-01" version="1">MVFPNGFSIKTDRDNFHDILNLYLFSYKYGVDIGSNWKFDEKYNILITPSGIKFSIKGFDPVIFSETFLYDIHFIDYNLENKKIIQAGAYVGETALYYAKRGAYVYAFEPQLDCYNIALTNLKLNPELASRVVLRNWAIGEDGEIEFPNTRCNGGTSYFDSYKDRIRVRSVSISTVLEEFNISQPDILDLDIKGGEFRVINDKSIQKFSIVRIEYNTVIDNKKLGDVTYLIEKLRSYGFTKIRIYKHNELPFDLSINGVIEARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XI2</accession>
    <name>Q97XI2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1758</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90336">
        <property type="entry name" value="H90336"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XI2"/>
    <dbReference type="PaxDb" id="273057-SSO1758"/>
    <dbReference type="EnsemblBacteria" id="AAK41951">
        <property type="protein sequence ID" value="AAK41951"/>
        <property type="gene ID" value="SSO1758"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1758"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1801"/>
    <dbReference type="eggNOG" id="arCOG01424">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155676_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XI2"/>
    <dbReference type="PhylomeDB" id="Q97XI2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38753">
        <property type="entry name" value="SLR1441 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38753:SF1">
        <property type="entry name" value="SLR1441 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="111" mass="12967" checksum="C29888408C3290AF" modified="2001-10-01" version="1">MLKLYRKALELHGVDPKRVEHGTIVDTVGIIEKGRSFEVVFYETDDYIYVFEIKNFSDKGVIDQVFIRKKLFSALYSKPLKVFVVTNYIEREVKEKLEKEGVEIIASYVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-10-02" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XL5</accession>
    <name>Q97XL5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRISPR system endoribonuclease Csx1-like HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90332">
        <property type="entry name" value="E90332"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923674.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XL5"/>
    <dbReference type="PaxDb" id="273057-SSO1711"/>
    <dbReference type="EnsemblBacteria" id="AAK41916">
        <property type="protein sequence ID" value="AAK41916"/>
        <property type="gene ID" value="SSO1711"/>
    </dbReference>
    <dbReference type="GeneID" id="1454695"/>
    <dbReference type="KEGG" id="sso:SSO1711"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1760"/>
    <dbReference type="eggNOG" id="arCOG03433">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1782573_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XL5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3740.10">
        <property type="entry name" value="SSO1389-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027419">
        <property type="entry name" value="CRISPR-assoc_Csx1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019016">
        <property type="entry name" value="Csx1-like_HEPN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09455">
        <property type="entry name" value="Csx1_HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160980">
        <property type="entry name" value="SSO1389-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR system endoribonuclease Csx1-like HEPN" evidence="1">
        <location>
            <begin position="55"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="145" mass="17072" checksum="2414C693C9472C25" modified="2001-10-01" version="1">MKIEGKEIKINEKLYPDRAWVDVICDYVCSKVKDNTKEEIEEFGKILEKFSMNAGPLISREINIIYNLAKDLKEGERKQYSEIYSEDEEGRSKQLSEEELKRNFIAHAGLLKEYVIIEKKNGKIYVNYDESKIKELLEWSVLKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XM2</accession>
    <name>Q97XM2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90330">
        <property type="entry name" value="F90330"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XM2"/>
    <dbReference type="STRING" id="273057.SSO1691"/>
    <dbReference type="PaxDb" id="273057-SSO1691"/>
    <dbReference type="EnsemblBacteria" id="AAK41901">
        <property type="protein sequence ID" value="AAK41901"/>
        <property type="gene ID" value="SSO1691"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1691"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1743"/>
    <dbReference type="eggNOG" id="arCOG08805">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1529297_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XM2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="217" mass="25688" checksum="6C5C3E1D73D623FC" modified="2001-10-01" version="1">MDKEKFLNCAMIVKNGMKKKLDKNFTVTTLKTVRVSKKLVEKLDSILYKQNKNFNQLINEFIEEYVNYRNKLEQITREKPIIISSRFFKHLIDLLGEDLEKIKEKSYKLGKEWGKEYLLLWNNSNVSSTTLNDINIFIKYLKDIAEYSGLYKLDIIEKKGSLTVIFHHDYSNIFSALLAFYYKGIFEAFYDNYEISDIAINQNNFIISITMKNLDHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XQ1</accession>
    <name>Q97XQ1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1658</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90327">
        <property type="entry name" value="A90327"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923553.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XQ1"/>
    <dbReference type="STRING" id="273057.SSO1658"/>
    <dbReference type="PaxDb" id="273057-SSO1658"/>
    <dbReference type="EnsemblBacteria" id="AAK41872">
        <property type="protein sequence ID" value="AAK41872"/>
        <property type="gene ID" value="SSO1658"/>
    </dbReference>
    <dbReference type="GeneID" id="1454651"/>
    <dbReference type="KEGG" id="sso:SSO1658"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1708"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="InParanoid" id="Q97XQ1"/>
    <dbReference type="PhylomeDB" id="Q97XQ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04762">
        <property type="entry name" value="HTH_MerR-trunc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2170:FF:000002">
        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1390:FF:000002">
        <property type="entry name" value="ORF1 in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="55"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="193" mass="22589" checksum="F2D053689E7F7B4B" modified="2001-10-01" version="1">MLRPKEVCQRLGISYPTLREYVKKGYIKPVILQSGKWRFNEEDVEKLMGIVKRRKVVLYARVSSKQKDELVNQVKYLEEQVKEYDLVITDIGSGLNMKRKGFLKLLRMILNNEVSRVVIAYPDRLVRFGFEILEETCKAHGCEIVVLNQEDKTPEEELVEDLVSVLVSFSEKLYGMRSHKYEKVKKCAEELKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XT9</accession>
    <name>Q97XT9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1618</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90322">
        <property type="entry name" value="B90322"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923520.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XT9"/>
    <dbReference type="STRING" id="273057.SSO1618"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1618"/>
    <dbReference type="DNASU" id="1454612"/>
    <dbReference type="EnsemblBacteria" id="AAK41833">
        <property type="protein sequence ID" value="AAK41833"/>
        <property type="gene ID" value="SSO1618"/>
    </dbReference>
    <dbReference type="GeneID" id="1454612"/>
    <dbReference type="KEGG" id="sso:SSO1618"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1660"/>
    <dbReference type="eggNOG" id="arCOG01417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053640_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XT9"/>
    <dbReference type="PhylomeDB" id="Q97XT9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="229"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="402" mass="46439" checksum="9882F1E69C86112B" modified="2001-10-01" version="1">MLSLGLADSRAISKANSGSERHVREVIKRLSLRYKLYYLPTTHAFLDAISEDQLKEIKKFAKVPSFFEYLLDKKLGKVSLLRELFTFSPLAKELVREYEREIGILDFIYVPHTYRIQLMPSILFSKLSRGKYGMLLMTDPHNSLLEKESFFKCVNVWSKIWFSRKSAIEFCSFQRLQNIAFLLKAIKTKPSFISVMNKCALNYTGLPKYFNTKVLSPPHAFNNLALKYRNFDKEDYLVFLGRINTAKGANEALEVGKHVKLKMIGYKEQEFIVKQARSFGIEVIENVSEEEKFEILSKARALVLPSHQESFSVTILEALAVGTPVITYDLPSLTSTYNFKPVFFVKEFDVKGLVNTALEVYNMKKIEEMFNDERLEEFIKMHSSWDNVSNAVDKLITEAIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XV9</accession>
    <name>Q97XV9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ORF in partial transposon ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO8456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90319">
        <property type="entry name" value="C90319"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XV9"/>
    <dbReference type="STRING" id="273057.SSO8456"/>
    <dbReference type="PaxDb" id="273057-SSO8456"/>
    <dbReference type="EnsemblBacteria" id="AAK41810">
        <property type="protein sequence ID" value="AAK41810"/>
        <property type="gene ID" value="SSO8456"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8456"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1638"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184190_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XV9"/>
    <dbReference type="PhylomeDB" id="Q97XV9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="75" mass="8736" checksum="39FCF6014BE6FAB4" modified="2001-10-01" version="1">MIRELKALGMEKSSFLTWFRNKGFIILKAFSLLVVLLLKYFTGLNLGAKRIARLIKSIYQASGEIKKLFKRKRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XY0</accession>
    <name>Q97XY0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DsrP protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1578</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NrfD family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XY0"/>
    <dbReference type="STRING" id="273057.SSO1578"/>
    <dbReference type="PaxDb" id="273057-SSO1578"/>
    <dbReference type="EnsemblBacteria" id="AAK41789">
        <property type="protein sequence ID" value="AAK41789"/>
        <property type="gene ID" value="SSO1578"/>
    </dbReference>
    <dbReference type="GeneID" id="7809903"/>
    <dbReference type="KEGG" id="sso:SSO1578"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1613"/>
    <dbReference type="eggNOG" id="arCOG02026">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045348_3_1_2"/>
    <dbReference type="InParanoid" id="Q97XY0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052049">
        <property type="entry name" value="Electron_transfer_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005614">
        <property type="entry name" value="NrfD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34856">
        <property type="entry name" value="PROTEIN NRFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34856:SF2">
        <property type="entry name" value="PROTEIN NRFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03916">
        <property type="entry name" value="NrfD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="5"/>
            <end position="26"/>
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            <end position="71"/>
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    </feature>
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            <end position="110"/>
        </location>
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            <end position="154"/>
        </location>
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            <end position="190"/>
        </location>
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            <end position="235"/>
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            <end position="277"/>
        </location>
    </feature>
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            <end position="321"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="431" mass="48700" checksum="D79DA3132B40E3A0" modified="2001-10-01" version="1">MNKFLLAWTIIFIIIGGGILLYGMSFNPMSWANGLVTFTEPNDEPIPWGLLVIGYMFFGVIGTGVSTYNSLYELFNKNHPEKNPFKNISLRNEWIALTVLIPGWIMVFASTYKPGEAVYIYTSFRDTSRIAWNGLLYALVGIGIILEILALIGEKRREYKDPISRLLAWLSSKSVLILIVGYAILMELILDANLGSVFGYLSTWVYEFGPFMSMLFVILSFYSGIAMLSFMTPLYNRFRKVKEDPNSLSIHEMYKILGRDGVLATIAVGFSLLWWLWLTATNQQTSAWAELMISGPYSVVFLIGGVLVGVIIPIILYGLVYKRGSSHMLVTSSIFTLLGMFSIITIADIIPQSITWYYSVSPISPSNSNWVYELRSIFNNGPSWTHLPFNISFYDIVFFIGSVLLLLGIYTLGVILLPLEEDEKPRHLIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XY4</accession>
    <name>Q97XY4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitric oxide reductase, subunit I (Cytochrome B) (NorB-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">norB-1</name>
        <name evidence="2" type="ordered locus">SSO1571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99316">
        <property type="entry name" value="A99316"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923475.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XY4"/>
    <dbReference type="STRING" id="273057.SSO1571"/>
    <dbReference type="PaxDb" id="273057-SSO1571"/>
    <dbReference type="EnsemblBacteria" id="AAK41784">
        <property type="protein sequence ID" value="AAK41784"/>
        <property type="gene ID" value="SSO1571"/>
    </dbReference>
    <dbReference type="GeneID" id="1454563"/>
    <dbReference type="KEGG" id="sso:SSO1571"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1607"/>
    <dbReference type="eggNOG" id="arCOG04703">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021582_1_0_2"/>
    <dbReference type="InParanoid" id="Q97XY4"/>
    <dbReference type="PhylomeDB" id="Q97XY4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.210.10:FF:000019">
        <property type="entry name" value="Nitric oxide reductase large subunit-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422:SF38">
        <property type="entry name" value="CYTOCHROME B SUBUNIT OF NITRIC OXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="Cytochrome c oxidase subunit I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
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        <location>
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            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="174"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="359"/>
        </location>
    </feature>
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        <location>
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            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="449"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="499"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="534" mass="58446" checksum="4E9C314AF3984962" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XZ7</accession>
    <name>Q97XZ7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1560</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HIBADH-related family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90314">
        <property type="entry name" value="G90314"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988658.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XZ7"/>
    <dbReference type="FunCoup" id="Q97XZ7">
        <property type="interactions" value="216"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1560"/>
    <dbReference type="PaxDb" id="273057-SSO1560"/>
    <dbReference type="EnsemblBacteria" id="AAK41774">
        <property type="protein sequence ID" value="AAK41774"/>
        <property type="gene ID" value="SSO1560"/>
    </dbReference>
    <dbReference type="GeneID" id="1454553"/>
    <dbReference type="KEGG" id="sso:SSO1560"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1594"/>
    <dbReference type="eggNOG" id="arCOG00247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035117_1_0_2"/>
    <dbReference type="InParanoid" id="Q97XZ7"/>
    <dbReference type="PhylomeDB" id="Q97XZ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002204">
        <property type="entry name" value="3-OH-isobutyrate_DH-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43060">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE-LIKE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43060:SF15">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE-LIKE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00895">
        <property type="entry name" value="3_HYDROXYISOBUT_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="4">
        <location>
            <begin position="2"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyisobutyrate dehydrogenase-like NAD-binding" evidence="5">
        <location>
            <begin position="164"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="289" mass="31025" checksum="4EEA33390300E7C7" modified="2001-10-01" version="1">MKVGFIGLGIMGFPMASNLLKAGYDLTVYNRTIEKAEKLGKMGAKVAHSPKEVAEVSEIVISMVTDAPDVEEVLFGENGVVKSNKRGLIFVDMSTNSPEFAKKVTKRLSEYGMEFLDAPVTGGDKGAREGTLTIMVGGKEDVFKRVEPIFKAMGKNIIHVGDVGSGQALKLCNQVVVALNMVSVVEGLLLARSLGIDDDKLFSVLSTGAANSFTVQYYLPKIMKGDLNPGFKAAHLKKDLKYAMEIANSKSLPLLGTSLALQLYNAMVSLGIGELGTQGLVKVYEKLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y07</accession>
    <name>Q97Y07_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial transposase ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90313">
        <property type="entry name" value="E90313"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923460.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y07"/>
    <dbReference type="PaxDb" id="273057-SSO1546"/>
    <dbReference type="EnsemblBacteria" id="AAK41764">
        <property type="protein sequence ID" value="AAK41764"/>
        <property type="gene ID" value="SSO1546"/>
    </dbReference>
    <dbReference type="GeneID" id="1454543"/>
    <dbReference type="KEGG" id="sso:SSO1546"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1584"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y07"/>
    <dbReference type="PhylomeDB" id="Q97Y07"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="79"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="289" mass="33667" checksum="ADE2356B4AD1D3C2" modified="2001-10-01" version="1">MQVYETGFKTELKSLASYFIVNNLNVISQELDTRIADAAPFLLTGNREKEYLKVVREGEKVMSQGERSFKFYPHVKVEGKTTIVAVDETAITVGEKESRKVEGFQLYNGKRKGVKLRSVDLVFPLRLSLLEEIADLRSDTPSQFLMRAVSEVAQHLKIDYVVADAGFLNLKVIKEMPVKTVVGGKTNLKGFKELSNVSLTEKKCEVNDRVYVAYRVLKYNDLYYYEVIYVKEKPRHFIFVTNFNGDPYKLAELYRLRWQSEEGFKVRKARIRYVSAFRKFMAYRQRIYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y75</accession>
    <name>Q97Y75_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1464</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90304">
        <property type="entry name" value="E90304"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989152.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y75"/>
    <dbReference type="PaxDb" id="273057-SSO1464"/>
    <dbReference type="EnsemblBacteria" id="AAK41692">
        <property type="protein sequence ID" value="AAK41692"/>
        <property type="gene ID" value="SSO1464"/>
    </dbReference>
    <dbReference type="GeneID" id="1454471"/>
    <dbReference type="KEGG" id="sso:SSO1464"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1496"/>
    <dbReference type="eggNOG" id="arCOG01937">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_523407_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y75"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="520" mass="57967" checksum="2B467CFCF94B41C0" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YF0</accession>
    <name>Q97YF0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thermopsin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90294">
        <property type="entry name" value="B90294"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923362.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YF0"/>
    <dbReference type="STRING" id="273057.SSO1375"/>
    <dbReference type="PaxDb" id="273057-SSO1375"/>
    <dbReference type="EnsemblBacteria" id="AAK41609">
        <property type="protein sequence ID" value="AAK41609"/>
        <property type="gene ID" value="SSO1375"/>
    </dbReference>
    <dbReference type="GeneID" id="1454388"/>
    <dbReference type="KEGG" id="sso:SSO1375"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1380"/>
    <dbReference type="eggNOG" id="arCOG06052">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_445254_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YF0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="588"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="67767" checksum="F478840A4CC89BA3" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YG1</accession>
    <name>Q97YG1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1048</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1362</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90292">
        <property type="entry name" value="F90292"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923354.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YG1"/>
    <dbReference type="EnsemblBacteria" id="AAK41597">
        <property type="protein sequence ID" value="AAK41597"/>
        <property type="gene ID" value="SSO1362"/>
    </dbReference>
    <dbReference type="GeneID" id="71027432"/>
    <dbReference type="KEGG" id="sso:SSO1362"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1368"/>
    <dbReference type="HOGENOM" id="CLU_124929_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YG1"/>
    <dbReference type="PhylomeDB" id="Q97YG1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13565">
        <property type="entry name" value="HTH_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="147" mass="17436" checksum="7C615D69B7282C0F" modified="2001-10-01" version="1">MQDLVQAYKEEKNARIKERILAVKLHVVDGKSEKEVSKMLNKGYSTIKLWIGKYKKEGLDGLKDKPRSGRPRKVEEEKIKQILEDKPQKYGIQQEYWTMKTLKIALQEQGIEYKKSRLYELVHELGYNLVKPRPTNIQAEKDKWEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YJ5</accession>
    <name>Q97YJ5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Restriction endonuclease related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90288">
        <property type="entry name" value="E90288"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923321.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YJ5"/>
    <dbReference type="STRING" id="273057.SSO1328"/>
    <dbReference type="REBASE" id="5092">
        <property type="entry name" value="SsfMcrBP"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1328"/>
    <dbReference type="EnsemblBacteria" id="AAK41564">
        <property type="protein sequence ID" value="AAK41564"/>
        <property type="gene ID" value="SSO1328"/>
    </dbReference>
    <dbReference type="GeneID" id="1454343"/>
    <dbReference type="KEGG" id="sso:SSO1328"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1328"/>
    <dbReference type="eggNOG" id="arCOG03779">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_465911_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YJ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052934">
        <property type="entry name" value="Methyl-DNA_Rec/Restrict_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37291">
        <property type="entry name" value="5-METHYLCYTOSINE-SPECIFIC RESTRICTION ENZYME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37291:SF1">
        <property type="entry name" value="TYPE IV METHYL-DIRECTED RESTRICTION ENZYME ECOKMCRB SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="266"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="559" mass="64938" checksum="D9DAF152C8044E72" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YK7</accession>
    <name>Q97YK7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1316</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99287">
        <property type="entry name" value="A99287"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YK7"/>
    <dbReference type="STRING" id="273057.SSO1316"/>
    <dbReference type="PaxDb" id="273057-SSO1316"/>
    <dbReference type="EnsemblBacteria" id="AAK41552">
        <property type="protein sequence ID" value="AAK41552"/>
        <property type="gene ID" value="SSO1316"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1316"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1317"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_8_1_2"/>
    <dbReference type="InParanoid" id="Q97YK7"/>
    <dbReference type="PhylomeDB" id="Q97YK7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="181" mass="21835" checksum="39904B2715835185" modified="2001-10-01" version="1">MRALKAQLRLACEVYNTLRWADTYFYQRDGKGLTQTELRQMALDLRKQDEEYKQLHSQVVQQIVDRFYEAKKRFMDGLARFPREKKPHKYYSLVYSQSGWKVLSLREIRRKSNHKKKLMKLYLSHLGVFNVIVHRDFPLDKVKRIIVKLEPSGRIYIIFVVDYEFKALPSTWKSCRDRRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YQ7</accession>
    <name>Q97YQ7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter, ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1266</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90281">
        <property type="entry name" value="A90281"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990022.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YQ7"/>
    <dbReference type="FunCoup" id="Q97YQ7">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1266"/>
    <dbReference type="PaxDb" id="273057-SSO1266"/>
    <dbReference type="EnsemblBacteria" id="AAK41504">
        <property type="protein sequence ID" value="AAK41504"/>
        <property type="gene ID" value="SSO1266"/>
    </dbReference>
    <dbReference type="GeneID" id="1454283"/>
    <dbReference type="KEGG" id="sso:SSO1266"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1263"/>
    <dbReference type="eggNOG" id="arCOG00196">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q97YQ7"/>
    <dbReference type="PhylomeDB" id="Q97YQ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF5">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="3"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="286" mass="32335" checksum="A110813F5E53271F" modified="2001-10-01" version="1">MTIEVEDLRKKFAGKEVLKGVSFKLRSGSITGFIGPNGAGKTTTIKILSGLLRKDGGFVKVFGEDPWDNPKVMERISVIFTNLIHPQENTVGEYLMDLGNVYGKKREIINYLIEEFKLTPHLNKRLYQLSSGLAQRVQLVAALIKDVELIIADEPTANLDPSFRIEFYEVVKELNRKNGVTFFISSHILSELEKVITDVIFINDGRITYSGRMNKALSDTEEEEIYVMVDDTDRALKLLGGILEGPYIKVKGKLREIVDKLDDNGIEIISIRRFSLDDAFKKFSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YR3</accession>
    <name>Q97YR3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mandelate racemase /muconate lactonizing enzyme related protein (MR/MLE)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1258</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90280">
        <property type="entry name" value="A90280"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991888.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YR3"/>
    <dbReference type="STRING" id="273057.SSO1258"/>
    <dbReference type="PaxDb" id="273057-SSO1258"/>
    <dbReference type="EnsemblBacteria" id="AAK41496">
        <property type="protein sequence ID" value="AAK41496"/>
        <property type="gene ID" value="SSO1258"/>
    </dbReference>
    <dbReference type="GeneID" id="1454275"/>
    <dbReference type="KEGG" id="sso:SSO1258"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1253"/>
    <dbReference type="eggNOG" id="arCOG01168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_3_0_2"/>
    <dbReference type="InParanoid" id="Q97YR3"/>
    <dbReference type="PhylomeDB" id="Q97YR3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03316">
        <property type="entry name" value="MR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046945">
        <property type="entry name" value="RHMD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794">
        <property type="entry name" value="ENOLASE SUPERFAMILY, MANDELATE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794:SF58">
        <property type="entry name" value="MITOCHONDRIAL ENOLASE SUPERFAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mandelate racemase/muconate lactonizing enzyme C-terminal" evidence="4">
        <location>
            <begin position="141"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="378" mass="42932" checksum="6D4E75DDD6835448" modified="2001-10-01" version="1">MKVKPFSLSIPFNTEPPSDWKDAWDVQLYVKVEDEKDYGWGEALVAGSGIINSYLSIINDIAIPLLSRVELSEPIDVRTILEKILFSAGNCGVVSGAISAVEMALWSLKARKSNVELYKLIGDKIRDSVKVYASFPRFGKIDDVLIATRKSLERGFDLVKLHQSPSTVLAAVKAIRENYKEVKIAIDLNSPFDNLDVAKEFVDKVHKYEIEWIEEPLWPPNDYDLLSKLTEFSPIPIAAGENEYSLYGFKKLIESGITYLQPDIAKVGGVTKFLEILDLASKYKVRVLPHDRPDASPISLIYTLNIGLVKSQIEMVEYTIADFPNDLFYSLPKFKDGYISPPENLEVVERNIEKYSYKNRLRILHFSDLENKLMRKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YX4</accession>
    <name>Q97YX4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2009-07" db="PDB data bank">
            <title>Crystal structure of a putative type 11 methyltransferase from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Bonanno J.B."/>
                <person name="Dickey M."/>
                <person name="Bain K.T."/>
                <person name="Chang S."/>
                <person name="Ozyurt S."/>
                <person name="Sauder J.M."/>
                <person name="Burley S.K."/>
                <person name="Almo S.C."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 18-176 IN COMPLEX WITH ZN(2+)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90271">
        <property type="entry name" value="C90271"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991664.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3I9F">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B=18-176"/>
    </dbReference>
    <dbReference type="PDBsum" id="3I9F"/>
    <dbReference type="AlphaFoldDB" id="Q97YX4"/>
    <dbReference type="SMR" id="Q97YX4"/>
    <dbReference type="STRING" id="273057.SSO1178"/>
    <dbReference type="PaxDb" id="273057-SSO1178"/>
    <dbReference type="DNASU" id="1454205"/>
    <dbReference type="EnsemblBacteria" id="AAK41426">
        <property type="protein sequence ID" value="AAK41426"/>
        <property type="gene ID" value="SSO1178"/>
    </dbReference>
    <dbReference type="GeneID" id="1454205"/>
    <dbReference type="KEGG" id="sso:SSO1178"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1174"/>
    <dbReference type="eggNOG" id="arCOG02702">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_16_1_2"/>
    <dbReference type="InParanoid" id="Q97YX4"/>
    <dbReference type="PhylomeDB" id="Q97YX4"/>
    <dbReference type="EvolutionaryTrace" id="Q97YX4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="36"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3I9F"/>
        </source>
    </evidence>
    <sequence length="176" mass="20815" checksum="154AFA8FA8F40393" modified="2001-10-01" version="1">MHHHRHYYPPEDFRRTFERPEEYLPNIFEGKKGVIVDYGCGNGFYCKYLLEFATKLYCIDINVIALKEVKEKFDSVITLSDPKEIPDNSVDFILFANSFHDMDDKQHVISEVKRILKDDGRVIIIDWRKENTGIGPPLSIRMDEKDYMGWFSNFVVEKRFNPTPYHFGLVLKRKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YZ3</accession>
    <name>Q97YZ3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl-CoA dehydrogenase related protein (Acd-like1)</fullName>
            <ecNumber evidence="3">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">acd-like1</name>
        <name evidence="3" type="ordered locus">SSO1156</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.3.99.-" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90268">
        <property type="entry name" value="H90268"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991588.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YZ3"/>
    <dbReference type="SMR" id="Q97YZ3"/>
    <dbReference type="STRING" id="273057.SSO1156"/>
    <dbReference type="PaxDb" id="273057-SSO1156"/>
    <dbReference type="EnsemblBacteria" id="AAK41407">
        <property type="protein sequence ID" value="AAK41407"/>
        <property type="gene ID" value="SSO1156"/>
    </dbReference>
    <dbReference type="GeneID" id="1454186"/>
    <dbReference type="KEGG" id="sso:SSO1156"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1150"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1044352_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YZ3"/>
    <dbReference type="PhylomeDB" id="Q97YZ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="2">
        <location>
            <begin position="157"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="266" mass="30030" checksum="602434243AC3082A" modified="2001-10-01" version="1">MLLDVETNEDLELIKNSFNEFLSREWNKYGSKKHEVSRDKIMEVFGKVKDLGIFQFIRETGLTNALLLNEIIGENLLPGIVAFSSMLGVDYLVSVGVNYVAEVDKAEAIVTPKGISNKNDVDVEEIESPDPSVRVYKIIEGEWRKSDFDFNKVILLASAQIIGHATAVLKETVEYSKNRIAFGKPIGSYQAIKHRIVDDALRIELVRSRYLVNPTSPERIFKHAYKKAFKAILNSIQSHGGIGFTADLDLHLHLKRVILLGKLFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z65</accession>
    <name>Q97Z65_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Intracellular proteinase</fullName>
            <ecNumber evidence="3">3.2.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1067</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C56 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.-.-" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90259">
        <property type="entry name" value="C90259"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989880.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z65"/>
    <dbReference type="FunCoup" id="Q97Z65">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1067"/>
    <dbReference type="MEROPS" id="C56.001"/>
    <dbReference type="PaxDb" id="273057-SSO1067"/>
    <dbReference type="EnsemblBacteria" id="AAK41330">
        <property type="protein sequence ID" value="AAK41330"/>
        <property type="gene ID" value="SSO1067"/>
    </dbReference>
    <dbReference type="GeneID" id="1454109"/>
    <dbReference type="KEGG" id="sso:SSO1067"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1064"/>
    <dbReference type="eggNOG" id="arCOG00769">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_44_4_2"/>
    <dbReference type="InParanoid" id="Q97Z65"/>
    <dbReference type="PhylomeDB" id="Q97Z65"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019172">
        <property type="term" value="F:glyoxalase III activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106044">
        <property type="term" value="P:guanine deglycation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03134">
        <property type="entry name" value="GATase1_PfpI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006286">
        <property type="entry name" value="C56_PfpI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01382">
        <property type="entry name" value="PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42733">
        <property type="entry name" value="DJ-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42733:SF2">
        <property type="entry name" value="DJ-1_THIJ_PFPI FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51276">
        <property type="entry name" value="PEPTIDASE_C56_PFPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="2">
        <location>
            <begin position="4"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="173" mass="19227" checksum="A27DE65B137C027E" modified="2001-10-01" version="1">MDSKKVLFLVGEEFEDIELLYPFYRVMEEGFKPVIAWKEANSKVIGKHGYTVISDIAFKDVRPEDYIALVIPGGRGPEHIRTLEEVKNITRKFFELKKPVAAICHGPQILISANLVKGRKLTSVNSIKDDVIAAGGIYVDNDVVVDENLISSRVPSDLPAFASTLIKALKSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z68</accession>
    <name>Q97Z68_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90258">
        <property type="entry name" value="F90258"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z68"/>
    <dbReference type="STRING" id="273057.SSO1062"/>
    <dbReference type="PaxDb" id="273057-SSO1062"/>
    <dbReference type="DNASU" id="1454104"/>
    <dbReference type="EnsemblBacteria" id="AAK41325">
        <property type="protein sequence ID" value="AAK41325"/>
        <property type="gene ID" value="SSO1062"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1062"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1058"/>
    <dbReference type="eggNOG" id="arCOG05971">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_808028_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z68"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="343" mass="38887" checksum="97E1676C904AF2C0" modified="2001-10-01" version="1">MIMNTKERSLLGSWIIWSVAYYMYYPFISIYLSRFVEESKLSLFFVVFQAVSLPLPLIGARLSKRNRILPIIIGMLVGGIGMIMLPFSRNILEATIFMSLNYFISLSLPSYYSLMSEVGEGTITRIWSLSILPSIIMPSLGGLLAQYFGLRLLFMIGGIVLATSFLPMVNSSYNSSSQSLLNFNITIRSFLPAILILPIAMEFPYIYLVVYNYFHLTKEYVGIIATSAEILGMLLTYLASKLIFRKKYLLSLSLFLFSLTSLYFLSPTIAIFFGCWEVIVPLTLEYFSSRKTVYDFALITTMQGLGWVLGYLIDYFIPNISLLLLSSSLIAFLLALMIVFTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z77</accession>
    <name>Q97Z77_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dolichyl-phosphooligosaccharide-protein glycotransferase</fullName>
            <ecNumber evidence="6">2.4.99.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Oligosaccharyl transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">SSO1052</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>an archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.</text>
            <dbReference type="EC" id="2.4.99.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endomembrane system</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.21" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90257">
        <property type="entry name" value="E90257"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923180.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z77"/>
    <dbReference type="FunCoup" id="Q97Z77">
        <property type="interactions" value="346"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1052"/>
    <dbReference type="CAZy" id="GT66">
        <property type="family name" value="Glycosyltransferase Family 66"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1052"/>
    <dbReference type="EnsemblBacteria" id="AAK41316">
        <property type="protein sequence ID" value="AAK41316"/>
        <property type="gene ID" value="SSO1052"/>
    </dbReference>
    <dbReference type="GeneID" id="1454095"/>
    <dbReference type="KEGG" id="sso:SSO1052"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1048"/>
    <dbReference type="eggNOG" id="arCOG02044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_376262_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z77"/>
    <dbReference type="PhylomeDB" id="Q97Z77"/>
    <dbReference type="BRENDA" id="2.4.99.18">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004576">
        <property type="term" value="F:oligosaccharyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF1">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="212"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="380"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="437"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="18">
        <location>
            <begin position="40"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AAK41316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="764" mass="83764" checksum="193F617C4D3C33E7" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z88</accession>
    <name>RNZ_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease Z</fullName>
            <shortName evidence="1">RNase Z</shortName>
            <ecNumber evidence="1">3.1.26.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA 3 endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNase Z</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnz</name>
        <name type="ordered locus">SSO1043</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase Z family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90256">
        <property type="entry name" value="B90256"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989186.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z88"/>
    <dbReference type="SMR" id="Q97Z88"/>
    <dbReference type="FunCoup" id="Q97Z88">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1043"/>
    <dbReference type="PaxDb" id="273057-SSO1043"/>
    <dbReference type="EnsemblBacteria" id="AAK41305">
        <property type="protein sequence ID" value="AAK41305"/>
        <property type="gene ID" value="SSO1043"/>
    </dbReference>
    <dbReference type="GeneID" id="1454084"/>
    <dbReference type="GeneID" id="44129972"/>
    <dbReference type="KEGG" id="sso:SSO1043"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1037"/>
    <dbReference type="eggNOG" id="arCOG00501">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031317_2_1_2"/>
    <dbReference type="InParanoid" id="Q97Z88"/>
    <dbReference type="PhylomeDB" id="Q97Z88"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07717">
        <property type="entry name" value="RNaseZ_ZiPD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01818">
        <property type="entry name" value="RNase_Z_BN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013471">
        <property type="entry name" value="RNase_Z/BN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000801">
        <property type="entry name" value="PRK00055.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02651">
        <property type="entry name" value="RNase_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018:SF2">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23023">
        <property type="entry name" value="Anti-Pycsar_Apyc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000155937" description="Ribonuclease Z">
        <location>
            <begin position="1"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01818"/>
        </source>
    </evidence>
    <sequence length="291" mass="33097" checksum="C6AE5934826E3A3D" modified="2001-10-01" version="1">MIQIFFLGTGAGSPSKKRKLPAFLVRREGVNILLDCGEGTQYTLMNYKLGINSIKIIGISHMHGDHVFGLLGVIASMGLLDRKETLYILGPRKLKDFLYTSFEYSKFNPSFKIEFIDNYNDENITISTFKTCHTIESQGYLIMEKDRVKIDEEKLEKEKIRDWRVIRKLKEGKTVEYNGKLLKPEDYLVIKRGSKVAYTGDTMPCQSVVDSVKGVDILIHDSTFLDEPSAYTYGHSNVTDAAKIALEASVKLLALTHISPRYEDVSEHLKMARRIFPKSILPDDLSYITIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZC8</accession>
    <name>Q97ZC8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator PadR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90251">
        <property type="entry name" value="B90251"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZC8"/>
    <dbReference type="STRING" id="273057.SSO0994"/>
    <dbReference type="PaxDb" id="273057-SSO0994"/>
    <dbReference type="EnsemblBacteria" id="AAK41265">
        <property type="protein sequence ID" value="AAK41265"/>
        <property type="gene ID" value="SSO0994"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0994"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.991"/>
    <dbReference type="eggNOG" id="arCOG00001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_3_3_2"/>
    <dbReference type="InParanoid" id="Q97ZC8"/>
    <dbReference type="PhylomeDB" id="Q97ZC8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR YQJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252:SF7">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR YQJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="30"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="103" mass="12146" checksum="13F78AD265902FE9" modified="2001-10-01" version="1">MDKFFIHKFYIVLVVKKTEKILGGIITLLILKTLLEEPKHGYELEKIIREKLDYKLPEGSIYVILKNLERRGLIKPQVMKNERGHDIKKYIITEEGKKILPIT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2025-06-18" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZG3</accession>
    <name>RIFK_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Riboflavin kinase</fullName>
            <shortName evidence="1">RFK</shortName>
            <ecNumber evidence="1">2.7.1.161</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">CTP-dependent riboflavin kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">CTP:riboflavin 5'-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Flavokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ribK</name>
        <name type="ordered locus">SSO0955</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>riboflavin + CTP = CDP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25021"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.1.161"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal riboflavin kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.161" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90246">
        <property type="entry name" value="F90246"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZG3"/>
    <dbReference type="SMR" id="Q97ZG3"/>
    <dbReference type="FunCoup" id="Q97ZG3">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0955"/>
    <dbReference type="PaxDb" id="273057-SSO0955"/>
    <dbReference type="EnsemblBacteria" id="AAK41229">
        <property type="protein sequence ID" value="AAK41229"/>
        <property type="gene ID" value="SSO0955"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0955"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.951"/>
    <dbReference type="eggNOG" id="arCOG01904">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088476_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZG3"/>
    <dbReference type="PhylomeDB" id="Q97ZG3"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00929"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01285">
        <property type="entry name" value="Riboflavin_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039063">
        <property type="entry name" value="RibK_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023470">
        <property type="entry name" value="Riboflavin_kinase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023602">
        <property type="entry name" value="Riboflavin_kinase_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706:SF1">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01982">
        <property type="entry name" value="CTP-dep_RFKase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000322079" description="Riboflavin kinase">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="54"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="155"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01285"/>
        </source>
    </evidence>
    <sequence length="175" mass="19586" checksum="AAB11E7C38199473" modified="2001-10-01" version="1">MEDDKLIVRIISKEGEIIRLTEEGERFLSSCLSSLKDAILSLHSIEIKGNIVSGLGEGKIFLSMEYYKSQINKIMGFDPFPGTLNIVIYDKASLENRLLLDSSPSLMVPEYKQKDRVLGAVKLYPAAINKLTPAAVVIPLRTTHPKSVIEIISPFHLREKLNLKDGDEVTIEVYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZG6</accession>
    <name>Q97ZG6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase BadF/BadG/BcrA/BcrD type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90246">
        <property type="entry name" value="A90246"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZG6"/>
    <dbReference type="FunCoup" id="Q97ZG6">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0950"/>
    <dbReference type="PaxDb" id="273057-SSO0950"/>
    <dbReference type="EnsemblBacteria" id="AAK41224">
        <property type="protein sequence ID" value="AAK41224"/>
        <property type="gene ID" value="SSO0950"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0950"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.946"/>
    <dbReference type="eggNOG" id="arCOG03659">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016274_1_0_2"/>
    <dbReference type="InParanoid" id="Q97ZG6"/>
    <dbReference type="PhylomeDB" id="Q97ZG6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045127">
        <property type="term" value="F:N-acetylglucosamine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039758">
        <property type="entry name" value="NAGK-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12862">
        <property type="entry name" value="BADF TYPE ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12862:SF0">
        <property type="entry name" value="N-ACETYL-D-GLUCOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase BadF/BadG/BcrA/BcrD type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="286" mass="32278" checksum="71D00DBE5D978028" modified="2001-10-01" version="1">MFISFVEYLLIDAGGTSTKVFVHDGEKIVRQYKYEPASVDKVGPYKSVLRLSNIVSIFNKKFDRIAISLAGIDSEVMAKEVKGQLYPRLSKYADKVIIEHDAHVVLMSNADKGCITIAGTGSIVYGFDGSQRIIKGDRGWLVGDICSGFWLGREFLHELLREFQGLSNDRSLTQFSTFKTEEDLVRFLYKNSCNPAKIAQFSVNLLNAIRQNNIKAIRILNNCMSEFSTLVQMVCKAVNSNVVYYFGGMYESPVYVSFFKRELEKKGIKSVKSRSVINGLLRLLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZG8</accession>
    <name>Q97ZG8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Archaeal coiled-coil protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90245">
        <property type="entry name" value="G90245"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992383.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZG8"/>
    <dbReference type="STRING" id="273057.SSO0947"/>
    <dbReference type="PaxDb" id="273057-SSO0947"/>
    <dbReference type="EnsemblBacteria" id="AAK41222">
        <property type="protein sequence ID" value="AAK41222"/>
        <property type="gene ID" value="SSO0947"/>
    </dbReference>
    <dbReference type="GeneID" id="1455189"/>
    <dbReference type="KEGG" id="sso:SSO0947"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.943"/>
    <dbReference type="eggNOG" id="arCOG01159">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080079_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZG8"/>
    <dbReference type="PhylomeDB" id="Q97ZG8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039604">
        <property type="entry name" value="Bfr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055545">
        <property type="entry name" value="DUF7121"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027:SF2">
        <property type="entry name" value="LEBERCILIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027">
        <property type="entry name" value="NUCLEAR SEGREGATION PROTEIN BFR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23435">
        <property type="entry name" value="DUF7121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="35563" checksum="B6450322DE047149" modified="2001-10-01" version="1">MSSISADTDELYKKISELKENIVRLKQLKLELIEKVKKMREKRREKIAKVKELTQQINVVRQEYKMKLEEFNQLKERRKNLIEIIQQIRKDFEEVKKLSSNNLGNPDSIERKIRELEWKLQTSSLTLEEEKKVIQRIAELEKKLQDAKKIMKIKEKRTEEKAELLAKKVELNTIRERIKTLINEITEKKNIIKKLVEERNKLRDEINGLNNEIENISKQIEELNVEIKNKSNELNEAQRALKSLQENPVQVNTTEFIEKKKKSIEEKLKQNKRLSFEEFLILYGEVDNKDGKDNSNIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-07-11" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZM1</accession>
    <name>OTC_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Ornithine carbamoyltransferase</fullName>
            <shortName>OTCase</shortName>
            <ecNumber>2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">argF</name>
        <name type="ordered locus">SSO0871</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>carbamoyl phosphate + L-ornithine = L-citrulline + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90237">
        <property type="entry name" value="H90237"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992290.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZM1"/>
    <dbReference type="SMR" id="Q97ZM1"/>
    <dbReference type="FunCoup" id="Q97ZM1">
        <property type="interactions" value="254"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0871"/>
    <dbReference type="PaxDb" id="273057-SSO0871"/>
    <dbReference type="EnsemblBacteria" id="AAK41159">
        <property type="protein sequence ID" value="AAK41159"/>
        <property type="gene ID" value="SSO0871"/>
    </dbReference>
    <dbReference type="GeneID" id="1455126"/>
    <dbReference type="GeneID" id="44129806"/>
    <dbReference type="KEGG" id="sso:SSO0871"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.880"/>
    <dbReference type="eggNOG" id="arCOG00912">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_3_2_2"/>
    <dbReference type="InParanoid" id="Q97ZM1"/>
    <dbReference type="PhylomeDB" id="Q97ZM1"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00112"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019240">
        <property type="term" value="P:citrulline biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000008">
        <property type="entry name" value="Ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001986">
        <property type="entry name" value="PRK00779.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF3">
        <property type="entry name" value="ORNITHINE TRANSCARBAMYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000113077" description="Ornithine carbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="54"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="132"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="231"/>
            <end position="232"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="266"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for structural integrity" evidence="1">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <feature type="site" description="Important for structural integrity" evidence="1">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="307" mass="34440" checksum="4C55C2516DD7D15B" modified="2001-10-01" version="1">MFRGKSLLCLLDFQTHEIEKMLEVSFLMKSYVYYNKVPNSLSGKRVALLFEKPSTRTRVSTELAVSLLGGIPIVLNKQDLQWSRGELIEDTGRVLGRVVNGIGARVLNHLTLVKLKESSGVPVFNLLSDLSHPLQALADLMTIRERFGNNLVKIAFVGDGSDNVLLSLMAIVAKLGLELHVATPKELKPRDDLYKIISEIAEDTGSVIEFHEDPYEAVRGVHVVYTDVWVSMGQENIAEQKKRMLQNYRVTADLMKYAVKDAIFMHCLPANRGEEVDPEVIDGSKSAVWDQAENRLYTTMAVFSLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZV4</accession>
    <name>Q97ZV4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF131 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO5909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90192">
        <property type="entry name" value="C90192"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922988.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZV4"/>
    <dbReference type="PaxDb" id="273057-SSO5909"/>
    <dbReference type="DNASU" id="1455604"/>
    <dbReference type="EnsemblBacteria" id="AAK40794">
        <property type="protein sequence ID" value="AAK40794"/>
        <property type="gene ID" value="SSO5909"/>
    </dbReference>
    <dbReference type="GeneID" id="15298033"/>
    <dbReference type="KEGG" id="sso:SSO5909"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.467"/>
    <dbReference type="eggNOG" id="arCOG02719">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149108_4_0_2"/>
    <dbReference type="InParanoid" id="Q97ZV4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002849">
        <property type="entry name" value="DUF131"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00304">
        <property type="entry name" value="TIGR00304 family membrane protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01998">
        <property type="entry name" value="DUF131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="9971" checksum="EEA3AFEA2C060448" modified="2001-10-01" version="1">MKLIAAGLTLIFLGFILLFLGVVLQVQTPQTTTSSSPQFAGLVLLGPIPIAFGNVPPSVLSNLIIVGVVFTIIMLIIYLIMFIIGRKTTRAPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZX6</accession>
    <name>Q97ZX6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein translation factor SUI1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO5866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90189">
        <property type="entry name" value="D90189"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZX6"/>
    <dbReference type="STRING" id="273057.SSO5866"/>
    <dbReference type="PaxDb" id="273057-SSO5866"/>
    <dbReference type="EnsemblBacteria" id="AAK40771">
        <property type="protein sequence ID" value="AAK40771"/>
        <property type="gene ID" value="SSO5866"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO5866"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.439"/>
    <dbReference type="eggNOG" id="arCOG04223">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3057318_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZX6"/>
    <dbReference type="PhylomeDB" id="Q97ZX6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUI1" evidence="1">
        <location>
            <begin position="24"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="53" mass="6132" checksum="02C7C6B3E0D791F5" modified="2001-10-01" version="1">MAENLCGGLPPDICEQLSKEEQFIKIKVEKRRYGKEVTIIEGIREVMILNLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZY1</accession>
    <name>Q97ZY1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0441</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90188">
        <property type="entry name" value="G90188"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988730.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZY1"/>
    <dbReference type="STRING" id="273057.SSO0441"/>
    <dbReference type="PaxDb" id="273057-SSO0441"/>
    <dbReference type="EnsemblBacteria" id="AAK40766">
        <property type="protein sequence ID" value="AAK40766"/>
        <property type="gene ID" value="SSO0441"/>
    </dbReference>
    <dbReference type="GeneID" id="1455576"/>
    <dbReference type="KEGG" id="sso:SSO0441"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.434"/>
    <dbReference type="eggNOG" id="arCOG05942">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1901920_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZY1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="134" mass="15574" checksum="4D530EA6F7D1FD3B" modified="2001-10-01" version="1">MNESDMVISRVLFDFETIINDHTEYLNELENLVAFPNPDIGKATRLVRRMRRVRKELFQGLEVIIQNIDKTTDNQIKEEALGLVNYLVIVGLKDEKELLNNLNNYLKGKGVNIEIDKDLEQIEKIMNSVSHLNF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-08-31" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZZ6</accession>
    <name>RS25_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small ribosomal subunit protein eS25</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">30S ribosomal protein S25e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps25e</name>
        <name type="ordered locus">SSO0425</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Located at the top of the head of the 30S subunit (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S small ribosomal subunit (PubMed:39753558). Interacts with uS13 (PubMed:39753558).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eukaryotic ribosomal protein eS25 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90186">
        <property type="entry name" value="F90186"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988758.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="c=1-110"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="c=1-110"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="c=1-110"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="c=1-110"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="c=1-110"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="c=1-110"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="c=1-110"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="c=1-110"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q97ZZ6"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q97ZZ6"/>
    <dbReference type="FunCoup" id="Q97ZZ6">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0425"/>
    <dbReference type="PaxDb" id="273057-SSO0425"/>
    <dbReference type="EnsemblBacteria" id="AAK40749">
        <property type="protein sequence ID" value="AAK40749"/>
        <property type="gene ID" value="SSO0425"/>
    </dbReference>
    <dbReference type="GeneID" id="1455559"/>
    <dbReference type="KEGG" id="sso:SSO0425"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.416"/>
    <dbReference type="eggNOG" id="arCOG04327">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171557_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZZ6"/>
    <dbReference type="PhylomeDB" id="Q97ZZ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004977">
        <property type="entry name" value="Ribosomal_eS25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006814">
        <property type="entry name" value="PRK09334.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12850">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03297">
        <property type="entry name" value="Ribosomal_S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000192893" description="Small ribosomal subunit protein eS25">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="110" mass="12346" checksum="2D4BDF555F89148D" modified="2001-10-01" version="1">MGGASKKPISTMEKRLKKEAEKQQKAEEKKKGPSKTGKEIISRAVTIDEETKKKVLDEIKKESIITPYALATKSGISISVARKILKELENQNVVKLYSKNRRLEIYIAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q980C8</accession>
    <name>Q980C8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LPS biosynthesis protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0388</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90182">
        <property type="entry name" value="D90182"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988811.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980C8"/>
    <dbReference type="STRING" id="273057.SSO0388"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO0388"/>
    <dbReference type="DNASU" id="1455525"/>
    <dbReference type="EnsemblBacteria" id="AAK40715">
        <property type="protein sequence ID" value="AAK40715"/>
        <property type="gene ID" value="SSO0388"/>
    </dbReference>
    <dbReference type="GeneID" id="1455525"/>
    <dbReference type="KEGG" id="sso:SSO0388"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.382"/>
    <dbReference type="eggNOG" id="arCOG01417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053640_0_0_2"/>
    <dbReference type="InParanoid" id="Q980C8"/>
    <dbReference type="PhylomeDB" id="Q980C8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="221"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="409" mass="47741" checksum="5FFED6728FC0B545" modified="2001-10-01" version="1">MTTLGVVYDKFLSPYFAGGGAVHAYEVTIRLKEHFKIVYYPSSPVLSWDKENVEKKAKELESQGIKVADEFYEILEEKRRIGRLKRFLFADKIAREFSKGFKVDADILYEPDHTSLDIFYLARDTKYGVTFHEPPFYNNSLRYFRRLVKFYGVNPYTGKGFHTRFLYNEYIKYLYKRLFKKVKKPTFLAGVSEAPLLESGLGGEVIKPGNAFNPSLLKFRNRGKEDYVVFWSRLNQDKGFHELPDILRIMEKRGGNKVRLILMGKFFDKYNERRFWSKVRKYDLRVDYKGFVKREELADIVSKAKVLIYPSHVDGFSLVALESLALGTPVVAYDIPAIKSVYGGLECVRIVNEFDKESMAENALKFYKMSEKEIEEIMNGDKLMEFLKLHSNWDNVANSVLKILKKYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q980M2</accession>
    <name>Q980M2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Multiprotein Bridging Factor (MBP-like), putative (MBP-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MBP-like</name>
        <name evidence="2" type="ordered locus">SSO0270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2014" name="Biochem. J." volume="462" first="373" last="384">
            <title>Archaeal MBF1 binds to 30S and 70S ribosomes via its helix-turn-helix domain.</title>
            <authorList>
                <person name="Blombach F."/>
                <person name="Launay H."/>
                <person name="Snijders A.P."/>
                <person name="Zorraquino V."/>
                <person name="Wu H."/>
                <person name="de Koning B."/>
                <person name="Brouns S.J."/>
                <person name="Ettema T.J."/>
                <person name="Camilloni C."/>
                <person name="Cavalli A."/>
                <person name="Vendruscolo M."/>
                <person name="Dickman M.J."/>
                <person name="Cabrita L.D."/>
                <person name="La Teana A."/>
                <person name="Benelli D."/>
                <person name="Londei P."/>
                <person name="Christodoulou J."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="24825021"/>
            <dbReference type="DOI" id="10.1042/bj20131474"/>
        </citation>
        <scope>STRUCTURE BY NMR OF 68-165</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90169">
        <property type="entry name" value="A90169"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990544.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2MEZ">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=68-165"/>
    </dbReference>
    <dbReference type="PDBsum" id="2MEZ"/>
    <dbReference type="AlphaFoldDB" id="Q980M2"/>
    <dbReference type="SMR" id="Q980M2"/>
    <dbReference type="FunCoup" id="Q980M2">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0270"/>
    <dbReference type="PaxDb" id="273057-SSO0270"/>
    <dbReference type="EnsemblBacteria" id="AAK40608">
        <property type="protein sequence ID" value="AAK40608"/>
        <property type="gene ID" value="SSO0270"/>
    </dbReference>
    <dbReference type="GeneID" id="1455418"/>
    <dbReference type="KEGG" id="sso:SSO0270"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.264"/>
    <dbReference type="eggNOG" id="arCOG01863">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130237_0_0_2"/>
    <dbReference type="InParanoid" id="Q980M2"/>
    <dbReference type="PhylomeDB" id="Q980M2"/>
    <dbReference type="EvolutionaryTrace" id="Q980M2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="DisProt" id="DP00856"/>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004451">
        <property type="entry name" value="MJ0586"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00270">
        <property type="entry name" value="multiprotein bridging factor aMBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="82"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2MEZ"/>
        </source>
    </evidence>
    <sequence length="165" mass="18563" checksum="9ABB4A46D1D4B709" modified="2001-10-01" version="1">MQANSEEYCELCGSQIRGKGITVSYEGSIITVCNSCYDRIRKHAVIVKADSKKSETRKKATLKPPKMENAELEIVTDYYKIIKTAREQLGISQQQLAQKLKVSENIVKRFESGKLKPTISQARQLEKILGIKLVTPLENNEESEKEFDDTGLTLGDVVNIKEGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q980N2</accession>
    <name>Q980N2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulatory protein, AsnC family, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO5522</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90167">
        <property type="entry name" value="G90167"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990524.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980N2"/>
    <dbReference type="STRING" id="273057.SSO5522"/>
    <dbReference type="PaxDb" id="273057-SSO5522"/>
    <dbReference type="EnsemblBacteria" id="AAK40598">
        <property type="protein sequence ID" value="AAK40598"/>
        <property type="gene ID" value="SSO5522"/>
    </dbReference>
    <dbReference type="GeneID" id="42362043"/>
    <dbReference type="KEGG" id="sso:SSO5522"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.253"/>
    <dbReference type="eggNOG" id="arCOG01117">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170329_2_0_2"/>
    <dbReference type="InParanoid" id="Q980N2"/>
    <dbReference type="PhylomeDB" id="Q980N2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF6">
        <property type="entry name" value="REGULATORY PROTEIN ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="2">
        <location>
            <begin position="6"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="83" mass="9396" checksum="6C547304E9E901AB" modified="2001-10-01" version="1">MASAIVLINTDAGGEEEVFEKLKNMNEVVEAYVVYGVYDIVIKVETENMDKLKNFISNSIRKIPKVRSTLTMIIMEGRSVIKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Q5</accession>
    <name>RS28_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein eS28</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">30S ribosomal protein S28e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 5" type="primary">rps28e</name>
        <name type="ordered locus">SSO5479</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Located near the mRNA exit channel, it binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S small ribosomal subunit interacts with aS33.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40573" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40573.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90164">
        <property type="entry name" value="F90164"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_014511512.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="X=1-83"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="X=1-83"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="X=1-83"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="X=1-83"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="X=1-83"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="X=1-83"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="X=1-83"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="X=1-83"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q980Q5"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q980Q5"/>
    <dbReference type="FunCoup" id="Q980Q5">
        <property type="interactions" value="200"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO5479"/>
    <dbReference type="PaxDb" id="273057-SSO5479"/>
    <dbReference type="EnsemblBacteria" id="AAK40573">
        <property type="protein sequence ID" value="AAK40573"/>
        <property type="gene ID" value="SSO5479"/>
    </dbReference>
    <dbReference type="GeneID" id="1455383"/>
    <dbReference type="KEGG" id="sso:SSO5479"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.228"/>
    <dbReference type="eggNOG" id="arCOG04314">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178987_2_0_2"/>
    <dbReference type="InParanoid" id="Q980Q5"/>
    <dbReference type="PhylomeDB" id="Q980Q5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04457">
        <property type="entry name" value="S1_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000145">
        <property type="entry name" value="30S ribosomal protein S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00292">
        <property type="entry name" value="Ribosomal_eS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_eS28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028626">
        <property type="entry name" value="Ribosomal_eS28_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003080">
        <property type="entry name" value="PRK04007.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00961">
        <property type="entry name" value="RIBOSOMAL_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000136861" description="Small ribosomal subunit protein eS28">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="83" mass="9378" checksum="24ED720FF31B2DDF" modified="2002-03-27" version="2">MSEKTQQSQGSSIIEEFGFPAEVIQILDRTGVTGEVTQVRVRVLEGRDKGRILTRNVKGPVRVGDILILRETEREARKITTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q980S7</accession>
    <name>Q980S7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO0200</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90161">
        <property type="entry name" value="C90161"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990442.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980S7"/>
    <dbReference type="STRING" id="273057.SSO0200"/>
    <dbReference type="PaxDb" id="273057-SSO0200"/>
    <dbReference type="EnsemblBacteria" id="AAK40546">
        <property type="protein sequence ID" value="AAK40546"/>
        <property type="gene ID" value="SSO0200"/>
    </dbReference>
    <dbReference type="GeneID" id="1455356"/>
    <dbReference type="KEGG" id="sso:SSO0200"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.200"/>
    <dbReference type="eggNOG" id="arCOG01679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156332_0_0_2"/>
    <dbReference type="InParanoid" id="Q980S7"/>
    <dbReference type="PhylomeDB" id="Q980S7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:001284">
        <property type="entry name" value="ArsR family transcriptional regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051081">
        <property type="entry name" value="HTH_MetalResp_TranReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154:SF38">
        <property type="entry name" value="HTH ARSR-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ARSR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="99" mass="11091" checksum="2DF575CCB7326606" modified="2001-10-01" version="1">MELIIDDPEKIYEISKALSTMTRINILQLVSITPMSISELTEKLNMSKGNISSHISELENLGLVEVEYQNGIKGIKKIIKAKYDKIIIVLKTSNSSNEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q981A4</accession>
    <name>Q981A4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Terminal oxidase, subunit (DoxC)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">doxC</name>
        <name evidence="2" type="ordered locus">SSO0045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90144">
        <property type="entry name" value="C90144"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981A4"/>
    <dbReference type="STRING" id="273057.SSO0045"/>
    <dbReference type="PaxDb" id="273057-SSO0045"/>
    <dbReference type="EnsemblBacteria" id="AAK40410">
        <property type="protein sequence ID" value="AAK40410"/>
        <property type="gene ID" value="SSO0045"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0045"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.46"/>
    <dbReference type="eggNOG" id="arCOG05891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_805610_0_0_2"/>
    <dbReference type="InParanoid" id="Q981A4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="355" mass="39425" checksum="2F36B0503FD7D8F5" modified="2001-10-01" version="1">MIKVDRKEKFELYWVIYVIVLFAIVIGATAPAVYTVGGDLSSVQAGIIIPNTAVNEGKVIQGTLYAYQYYFAIKENGGSITSNELGSPYYYNIMVAHPGQYINLTMYAAPHMATANFYYPDYATHVDDVQIVPGLVQYAPVVVPNISGAFAFLNGEYNGPWFSYQEGLLLVLPYQGYMSQTDVSKYITQTHLAQTSTLVGDPYNPPIVVYNTTMTPTVDLVADQYAIFNNSVPGPTAVVLANSNVTVNMYIPTPNSDHNFLYNYSTSGTPYPVRNVYIGIYAVWWNGSITLVKQVPIPYDKTITFTFTANAPAYLYGLITPVYYNYNFMNMSNTLTGEQYGYVMGLWGTILVEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q981A8</accession>
    <name>Q981A8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiamine-phosphate synthase ThiN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90143">
        <property type="entry name" value="E90143"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989596.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981A8"/>
    <dbReference type="FunCoup" id="Q981A8">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0039"/>
    <dbReference type="PaxDb" id="273057-SSO0039"/>
    <dbReference type="EnsemblBacteria" id="AAK40404">
        <property type="protein sequence ID" value="AAK40404"/>
        <property type="gene ID" value="SSO0039"/>
    </dbReference>
    <dbReference type="GeneID" id="1455295"/>
    <dbReference type="KEGG" id="sso:SSO0039"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.40"/>
    <dbReference type="eggNOG" id="arCOG00021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105304_0_0_2"/>
    <dbReference type="InParanoid" id="Q981A8"/>
    <dbReference type="PhylomeDB" id="Q981A8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019293">
        <property type="entry name" value="ThiN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40730:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40730">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10120">
        <property type="entry name" value="ThiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine-phosphate synthase ThiN" evidence="1">
        <location>
            <begin position="14"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="190" mass="21766" checksum="727A1E75D41640B9" modified="2001-10-01" version="1">MQDESEREYVLKRLKEAVDIFVSNERAYLLIPEVRTNIGYAVSNAADANDVAAIPGRLTTAFKKVIYCMLPAFGASDHVARVILTVMKHDRNIRSAINLKYYREVIEKLPSQDLCIFDRSSEPKEVKYREHSTMNFMVDSCIRKLSKVPNYIVDLGDYGKEPSLFILDRDPVTVVNKSLELLKYISQDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q981B1</accession>
    <name>Q981B1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90143">
        <property type="entry name" value="B90143"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981B1"/>
    <dbReference type="STRING" id="273057.SSO0036"/>
    <dbReference type="PaxDb" id="273057-SSO0036"/>
    <dbReference type="DNASU" id="1455292"/>
    <dbReference type="EnsemblBacteria" id="AAK40401">
        <property type="protein sequence ID" value="AAK40401"/>
        <property type="gene ID" value="SSO0036"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0036"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.37"/>
    <dbReference type="eggNOG" id="arCOG08484">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1718296_0_0_2"/>
    <dbReference type="InParanoid" id="Q981B1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="155" mass="18352" checksum="18B92F15C2D326EE" modified="2001-10-01" version="1">MIRMDICVKARNDEEATRALQLNYNCVVYSRPLNNILTFIPDQYISDININNERTVVITDSLELINTLKSRNLKIAYRVNKPYMNILIYDYFVLEFIPKNIRDLRFYKGKVYIDNIDNLDIYSKLENLKISGVFTNNLDFIVNLKKLGLYKNRII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2025-06-18" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UWX3</accession>
    <name>PDXS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase subunit PdxS</fullName>
            <shortName evidence="1">PLP synthase subunit PdxS</shortName>
            <ecNumber evidence="1">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pdx1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdxS</name>
        <name type="ordered locus">SSO0570</name>
        <name type="ORF">C21_029</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the PdxT subunit. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">In the presence of PdxT, forms a dodecamer of heterodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90203">
        <property type="entry name" value="G90203"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923033.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UWX3"/>
    <dbReference type="SMR" id="Q9UWX3"/>
    <dbReference type="FunCoup" id="Q9UWX3">
        <property type="interactions" value="194"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0570"/>
    <dbReference type="PaxDb" id="273057-SSO0570"/>
    <dbReference type="EnsemblBacteria" id="AAK40886">
        <property type="protein sequence ID" value="AAK40886"/>
        <property type="gene ID" value="SSO0570"/>
    </dbReference>
    <dbReference type="GeneID" id="1454853"/>
    <dbReference type="GeneID" id="27426877"/>
    <dbReference type="KEGG" id="sso:SSO0570"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.579"/>
    <dbReference type="eggNOG" id="arCOG04075">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055352_1_0_2"/>
    <dbReference type="InParanoid" id="Q9UWX3"/>
    <dbReference type="PhylomeDB" id="Q9UWX3"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016843">
        <property type="term" value="F:amine-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04727">
        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000001">
        <property type="entry name" value="Pyridoxine biosynthesis protein PDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01824">
        <property type="entry name" value="PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003215">
        <property type="entry name" value="PRK04180.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01235">
        <property type="entry name" value="PDXS_SNZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000109448" description="Pyridoxal 5'-phosphate synthase subunit PdxS">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with D-ribose 5-phosphate" evidence="1">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="277"/>
            <end position="278"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01824"/>
        </source>
    </evidence>
    <sequence length="338" mass="37495" checksum="7EDA3672EA1BC837" modified="2000-05-01" version="1">MRLYELSFAQIEDFFYKLAEVKDIIKDHGLLEFLPEFKKLDSTVHTGTTRVKHAFPIFQKGGVVMDITNVQQAQIAEEAGAVAVMVLDKLPYDVRKSGGVARMADPKIIEEVMSSITIPVMAKVRIGHYYEAKLLEALGVDMIDESEVLTPADEEHHINKWEFSVPFVNGARNLGEALRRTSEGASMIRTKGEAGTGNVSEAVKHMKIINSEIRSLISMSEEDRVKKAREYQVPYQIVELTAKIKRLPIVNFAAGGIATPADAALMMWLGADGLFVGSGIFKSQDPDERAKAVVLAAACWEYPEIVLEAQKMISEQKSMMGIDIKSLKPEELLQVRGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX20</accession>
    <name>PUR7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="1">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purC</name>
        <name type="ordered locus">SSO0626</name>
        <name type="ORF">C08_023</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate + ATP = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90210">
        <property type="entry name" value="B90210"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991162.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX20"/>
    <dbReference type="SMR" id="Q9UX20"/>
    <dbReference type="FunCoup" id="Q9UX20">
        <property type="interactions" value="273"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0626"/>
    <dbReference type="PaxDb" id="273057-SSO0626"/>
    <dbReference type="EnsemblBacteria" id="AAK40937">
        <property type="protein sequence ID" value="AAK40937"/>
        <property type="gene ID" value="SSO0626"/>
    </dbReference>
    <dbReference type="GeneID" id="1454904"/>
    <dbReference type="GeneID" id="44129628"/>
    <dbReference type="KEGG" id="sso:SSO0626"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.633"/>
    <dbReference type="eggNOG" id="arCOG04421">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061495_2_0_2"/>
    <dbReference type="InParanoid" id="Q9UX20"/>
    <dbReference type="PhylomeDB" id="Q9UX20"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01415">
        <property type="entry name" value="SAICAR_synt_PurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000086">
        <property type="entry name" value="Phosphoribosylaminoimidazole-succinocarboxamide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033934">
        <property type="entry name" value="SAICAR_synt_PurC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050089">
        <property type="entry name" value="SAICAR_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43599">
        <property type="entry name" value="MULTIFUNCTIONAL PROTEIN ADE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43599:SF3">
        <property type="entry name" value="SI:DKEY-6E2.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000100919" description="Phosphoribosylaminoimidazole-succinocarboxamide synthase">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <sequence length="233" mass="27283" checksum="EE9A4A952EEB0B80" modified="2000-05-01" version="1">MEVNKISEGKTKIVYDFDKDHVLLEFKDDITAGDGQKRDILEGKGVLNAQTSAFFFRLLERNGIETHYVGMKDERTMIVKKLKMIPVEVVLRNIATGSIVKRLPIKEGEVFESPIVEFFLKDDLRHDPLLNYYHMEHLKLMNRKEAQHIEDIMMKVNDVLFNFIKSKGFILYDFKLEFGRLGDKLVIGDEISLDSMRIRDGNSRIYDKDLYRKGYDLKTVKSSYEEFLKRITS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXD0</accession>
    <name>RL15E_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>50S ribosomal protein L15e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rpl15e</name>
        <name type="ordered locus">SSO0742</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90222">
        <property type="entry name" value="G90222"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991318.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXD0"/>
    <dbReference type="SMR" id="Q9UXD0"/>
    <dbReference type="FunCoup" id="Q9UXD0">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0742"/>
    <dbReference type="PaxDb" id="273057-SSO0742"/>
    <dbReference type="EnsemblBacteria" id="AAK41038">
        <property type="protein sequence ID" value="AAK41038"/>
        <property type="gene ID" value="SSO0742"/>
    </dbReference>
    <dbReference type="GeneID" id="1455005"/>
    <dbReference type="KEGG" id="sso:SSO0742"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.738"/>
    <dbReference type="eggNOG" id="arCOG04209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080796_1_0_2"/>
    <dbReference type="InParanoid" id="Q9UXD0"/>
    <dbReference type="PhylomeDB" id="Q9UXD0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1120.10:FF:000002">
        <property type="entry name" value="50S ribosomal protein L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="entry name" value="Ribosomal protein l15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00256">
        <property type="entry name" value="Ribosomal_eL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_eL15_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_eL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020926">
        <property type="entry name" value="Ribosomal_eL15_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020925">
        <property type="entry name" value="Ribosomal_eL15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003269">
        <property type="entry name" value="PRK04243.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01194">
        <property type="entry name" value="RIBOSOMAL_L15E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000127586" description="Large ribosomal subunit protein eL15">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="216" mass="25727" checksum="7B49241289194BA3" modified="2000-05-01" version="1">MALSMYHYIENTWNSEEWKKSVLRQRLIKWRKEPSIVRLAKPTRLNRARSLGYKAKQGFVIVRVRVRRGGLNKPRPNKGRRPKRMGVYGYSPAKGYRWIAEERAARKYPNLEVLGSYYVGEDGLYKYYEIVMVDPSHPVIKSDPKHKWLQDPSNRNRVFRGLTSAGKKVRGLRKSRGLKGTVRYKWNRKQKEREEKKRHEASKYYKLQEYDKIPGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXF7</accession>
    <name>MOAC_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable cyclic pyranopterin monophosphate synthase</fullName>
            <ecNumber evidence="1">4.6.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Molybdenum cofactor biosynthesis protein C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">moaC</name>
        <name type="ordered locus">SSO0770</name>
        <name type="ORF">C40_006</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cyclic pyranopterin monophosphate (cPMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer; trimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MoaC family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.17" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90226">
        <property type="entry name" value="D90226"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991359.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXF7"/>
    <dbReference type="SMR" id="Q9UXF7"/>
    <dbReference type="FunCoup" id="Q9UXF7">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0770"/>
    <dbReference type="PaxDb" id="273057-SSO0770"/>
    <dbReference type="EnsemblBacteria" id="AAK41067">
        <property type="protein sequence ID" value="AAK41067"/>
        <property type="gene ID" value="SSO0770"/>
    </dbReference>
    <dbReference type="GeneID" id="1455034"/>
    <dbReference type="GeneID" id="44129775"/>
    <dbReference type="KEGG" id="sso:SSO0770"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.769"/>
    <dbReference type="eggNOG" id="arCOG01530">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074693_1_2_2"/>
    <dbReference type="InParanoid" id="Q9UXF7"/>
    <dbReference type="PhylomeDB" id="Q9UXF7"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01419">
        <property type="entry name" value="MoaC_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="entry name" value="Molybdopterin cofactor biosynthesis C (MoaC) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01224_A">
        <property type="entry name" value="MoaC_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023047">
        <property type="entry name" value="Mo_CF_biosynth-C_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023045">
        <property type="entry name" value="MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00581">
        <property type="entry name" value="moaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008999">
        <property type="entry name" value="PRK12343.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein C, MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000097864" description="Probable cyclic pyranopterin monophosphate synthase">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="67"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="103"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01224"/>
        </source>
    </evidence>
    <sequence length="149" mass="16838" checksum="7E1A6242382C8908" modified="2000-05-01" version="1">MSSEAKMVDISQKETVLREAEAEGFIKLKNDTIKRIIENEIEKGNVITVAKTAGIMAAKKTSELLPLCHLIPLENVDIDIKIENSGIRVRSKVKAHYKTGVEMEALVATSISLLTIWDMVKKYEKDENGKYPYTMIDDIKVIDKIKEKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="137" xmlns="https://uniprot.org/uniprot">
    <accession>O06456</accession>
    <name>PURE_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="1">N5-CAIR mutase</shortName>
            <ecNumber evidence="1">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purE</name>
        <name type="ordered locus">SSO1064</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="FEMS Microbiol. Lett." volume="154" first="173" last="180">
            <title>Identification and sequence analysis of Sulfolobus solfataricus purE and purK genes.</title>
            <authorList>
                <person name="Soerensen I.S."/>
                <person name="Dandanell G."/>
            </authorList>
            <dbReference type="PubMed" id="9311113"/>
            <dbReference type="DOI" id="10.1016/S0378-1097(97)00320-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="1"/>
    <dbReference type="EMBL" id="Y13143">
        <property type="protein sequence ID" value="CAA73604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90258">
        <property type="entry name" value="H90258"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989885.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O06456"/>
    <dbReference type="SMR" id="O06456"/>
    <dbReference type="FunCoup" id="O06456">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1064"/>
    <dbReference type="PaxDb" id="273057-SSO1064"/>
    <dbReference type="EnsemblBacteria" id="AAK41327">
        <property type="protein sequence ID" value="AAK41327"/>
        <property type="gene ID" value="SSO1064"/>
    </dbReference>
    <dbReference type="GeneID" id="1454106"/>
    <dbReference type="GeneID" id="44129998"/>
    <dbReference type="KEGG" id="sso:SSO1064"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1061"/>
    <dbReference type="eggNOG" id="arCOG02464">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094982_2_0_2"/>
    <dbReference type="InParanoid" id="O06456"/>
    <dbReference type="PhylomeDB" id="O06456"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23046:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23046">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001338">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000074990" description="N5-carboxyaminoimidazole ribonucleotide mutase">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <sequence length="158" mass="17096" checksum="3B125E90BF22707A" modified="1997-07-01" version="1">MPKVAVIMGSKNDWEYMREAVEILKQFGIDYEARVVSAHRTPEFMMQYAKEAEKRGIEVIIAGAGGAAHLPGMVASLTSLPVIGVPIPSKNLNGLDSLLSIVQMPYGVPVATVAIGGAKNAALLAIRILGIKYKELADKIKKFSEDMRNDVLSTRLEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1995-02-01" modified="2026-01-28" version="148" xmlns="https://uniprot.org/uniprot">
    <accession>P39476</accession>
    <accession>P81550</accession>
    <name>DN7D_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA-binding protein 7d</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>7 kDa DNA-binding protein d</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Sso7d</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">sso7d</name>
        <name type="synonym">sso7d-1</name>
        <name type="ordered locus">SSO10610</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1988" name="Biochim. Biophys. Acta" volume="950" first="193" last="203">
            <title>Isolation, characterization and microsequence analysis of a small basic methylated DNA-binding protein from the Archaebacterium, Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Choli T."/>
                <person name="Henning P."/>
                <person name="Wittmann-Liebold B."/>
                <person name="Reinhardt R."/>
            </authorList>
            <dbReference type="PubMed" id="3132977"/>
            <dbReference type="DOI" id="10.1016/0167-4781(88)90011-5"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-64</scope>
        <scope>DNA-BINDING</scope>
        <scope>SUBUNIT</scope>
        <scope>METHYLATION AT LYS-5; LYS-7; LYS-61; LYS-63 AND LYS-64</scope>
        <source>
            <strain>ATCC 35091 / DSM 1616 / IFO 15331 / JCM 8930 / P1</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1998" name="FEBS Lett." volume="432" first="141" last="144">
            <title>Isolation and structure of repressor-like proteins from the archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Oppermann U.C.T."/>
                <person name="Knapp S."/>
                <person name="Bonetto V."/>
                <person name="Ladenstein R."/>
                <person name="Joernvall H."/>
            </authorList>
            <dbReference type="PubMed" id="9720912"/>
            <dbReference type="DOI" id="10.1016/S0014-5793(98)00848-5"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-64</scope>
        <scope>METHYLATION AT LYS-5; LYS-7; LYS-61; LYS-63 AND LYS-64</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="1995" name="FEBS Lett." volume="360" first="187" last="190">
            <title>An 8.5-kDa ribonuclease from the extreme thermophilic archaebacterium Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Fusi P."/>
                <person name="Grisa M."/>
                <person name="Tedeschi G."/>
                <person name="Negri A."/>
                <person name="Guerritore A."/>
                <person name="Tortora P."/>
            </authorList>
            <dbReference type="PubMed" id="7875327"/>
            <dbReference type="DOI" id="10.1016/0014-5793(95)00098-T"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-64</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="1993" name="Eur. J. Biochem." volume="211" first="305" last="310">
            <title>Ribonucleases from the extreme thermophilic archaebacterium S. solfataricus.</title>
            <authorList>
                <person name="Fusi P."/>
                <person name="Tedeschi G."/>
                <person name="Aliverti A."/>
                <person name="Ronchi S."/>
                <person name="Tortora P."/>
                <person name="Guerritore A."/>
            </authorList>
            <dbReference type="PubMed" id="8425540"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1993.tb19899.x"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-58</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="1992" name="FEBS Lett." volume="303" first="27" last="30">
            <title>Isolation of a thermostable enzyme catalyzing disulfide bond formation from the archaebacterium Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Guagliardi A."/>
                <person name="Cerchia L."/>
                <person name="De Rosa M."/>
                <person name="Rossi M."/>
                <person name="Bartolucci S."/>
            </authorList>
            <dbReference type="PubMed" id="1592111"/>
            <dbReference type="DOI" id="10.1016/0014-5793(92)80470-2"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-13</scope>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="2992" last="2996">
            <title>Holliday junction resolution is modulated by archaeal chromatin components in vitro.</title>
            <authorList>
                <person name="Kvaratskhelia M."/>
                <person name="Wardleworth B.N."/>
                <person name="Bond C.S."/>
                <person name="Fogg J.M."/>
                <person name="Lilley D.M."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="11709558"/>
            <dbReference type="DOI" id="10.1074/jbc.M109496200"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="37274" last="37274">
            <title>The archaeal '7 kDa DNA-binding' proteins: extended characterization of an old gifted family.</title>
            <authorList>
                <person name="Kalichuk V."/>
                <person name="Behar G."/>
                <person name="Renodon-Corniere A."/>
                <person name="Danovski G."/>
                <person name="Obal G."/>
                <person name="Barbet J."/>
                <person name="Mouratou B."/>
                <person name="Pecorari F."/>
            </authorList>
            <dbReference type="PubMed" id="27853299"/>
            <dbReference type="DOI" id="10.1038/srep37274"/>
        </citation>
        <scope>DNA-BINDING</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="9">
        <citation type="journal article" date="1994" name="Nat. Struct. Biol." volume="1" first="808" last="819">
            <title>Solution structure and DNA-binding properties of a thermostable protein from the archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Baumann H."/>
                <person name="Knapp S."/>
                <person name="Lundbaeck T."/>
                <person name="Ladenstein R."/>
                <person name="Haerd T."/>
            </authorList>
            <dbReference type="PubMed" id="7634092"/>
            <dbReference type="DOI" id="10.1038/nsb1194-808"/>
        </citation>
        <scope>STRUCTURE BY NMR</scope>
        <scope>DNA-BINDING</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="10">
        <citation type="journal article" date="1998" name="Nat. Struct. Biol." volume="5" first="579" last="584">
            <title>Architecture of nonspecific protein-DNA interactions in the Sso7d-DNA complex.</title>
            <authorList>
                <person name="Agback P."/>
                <person name="Baumann H."/>
                <person name="Knapp S."/>
                <person name="Ladenstein R."/>
                <person name="Haerd T."/>
            </authorList>
            <dbReference type="PubMed" id="9665172"/>
            <dbReference type="DOI" id="10.1038/836"/>
        </citation>
        <scope>STRUCTURE BY NMR</scope>
        <scope>DNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="11">
        <citation type="journal article" date="1998" name="Nat. Struct. Biol." volume="5" first="782" last="786">
            <title>The crystal structure of the hyperthermophile chromosomal protein Sso7d bound to DNA.</title>
            <authorList>
                <person name="Gao Y.G."/>
                <person name="Su S.Y."/>
                <person name="Robinson H."/>
                <person name="Padmanabhan S."/>
                <person name="Lim L."/>
                <person name="McCrary B.S."/>
                <person name="Edmondson S.P."/>
                <person name="Shriver J.W."/>
                <person name="Wang A.H."/>
            </authorList>
            <dbReference type="PubMed" id="9731772"/>
            <dbReference type="DOI" id="10.1038/1822"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature (By similarity). Stimulates the Holliday junction cleavage activity of Hjc (PubMed:11709558).</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="4">Highly stable from pH 0 to pH 12.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="4">Hyperthermostable.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="4 5 6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the 7 kDa DNA-binding/endoribonuclease P2 family.</text>
    </comment>
    <comment type="sequence caution" evidence="10">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK42679" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42679.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90427">
        <property type="entry name" value="H90427"/>
    </dbReference>
    <dbReference type="PIR" id="S69085">
        <property type="entry name" value="S69085"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990119.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1BBX">
        <property type="method" value="NMR"/>
        <property type="chains" value="C/D=2-64"/>
    </dbReference>
    <dbReference type="PDB" id="1BNZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-64"/>
    </dbReference>
    <dbReference type="PDB" id="1C8C">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.45 A"/>
        <property type="chains" value="A=1-64"/>
    </dbReference>
    <dbReference type="PDB" id="1JIC">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=2-62"/>
    </dbReference>
    <dbReference type="PDB" id="5B02">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.21 A"/>
        <property type="chains" value="A/B/C/D=1-64"/>
    </dbReference>
    <dbReference type="PDB" id="5B03">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A/B/C/D=1-64"/>
    </dbReference>
    <dbReference type="PDB" id="5B0I">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.26 A"/>
        <property type="chains" value="A/B/C/D=1-64"/>
    </dbReference>
    <dbReference type="PDB" id="5B0J">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B/C/D=1-64"/>
    </dbReference>
    <dbReference type="PDB" id="5B0K">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.75 A"/>
        <property type="chains" value="A/B/C/D=1-64"/>
    </dbReference>
    <dbReference type="PDB" id="5B0L">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H=1-64"/>
    </dbReference>
    <dbReference type="PDB" id="5B0M">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.05 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H=1-64"/>
    </dbReference>
    <dbReference type="PDB" id="5GWV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B/C/D=1-64"/>
    </dbReference>
    <dbReference type="PDB" id="5GWW">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B/C/D=1-64"/>
    </dbReference>
    <dbReference type="PDB" id="5U1C">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.90 A"/>
        <property type="chains" value="A/B/C/D=1-64"/>
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    <dbReference type="PDB" id="6J8V">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.23 A"/>
        <property type="chains" value="A/B/C/D=1-64"/>
    </dbReference>
    <dbReference type="PDB" id="6J8W">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.35 A"/>
        <property type="chains" value="A/B/C/D=1-64"/>
    </dbReference>
    <dbReference type="PDB" id="6PUT">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="A/B/C/D=1-64"/>
    </dbReference>
    <dbReference type="PDB" id="6PUW">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="A/B/C/D=1-64"/>
    </dbReference>
    <dbReference type="PDB" id="6PUY">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B/C/D=1-64"/>
    </dbReference>
    <dbReference type="PDB" id="6PUZ">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B/C/D=1-64"/>
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    <dbReference type="PDB" id="6V3K">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="A/B/C/D=1-64"/>
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    <dbReference type="PDB" id="6VOY">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="A/B/C/D=1-64"/>
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    <dbReference type="PDBsum" id="1BBX"/>
    <dbReference type="PDBsum" id="1BNZ"/>
    <dbReference type="PDBsum" id="1C8C"/>
    <dbReference type="PDBsum" id="1JIC"/>
    <dbReference type="PDBsum" id="5B02"/>
    <dbReference type="PDBsum" id="5B03"/>
    <dbReference type="PDBsum" id="5B0I"/>
    <dbReference type="PDBsum" id="5B0J"/>
    <dbReference type="PDBsum" id="5B0K"/>
    <dbReference type="PDBsum" id="5B0L"/>
    <dbReference type="PDBsum" id="5B0M"/>
    <dbReference type="PDBsum" id="5GWV"/>
    <dbReference type="PDBsum" id="5GWW"/>
    <dbReference type="PDBsum" id="5U1C"/>
    <dbReference type="PDBsum" id="6J8V"/>
    <dbReference type="PDBsum" id="6J8W"/>
    <dbReference type="PDBsum" id="6PUT"/>
    <dbReference type="PDBsum" id="6PUW"/>
    <dbReference type="PDBsum" id="6PUY"/>
    <dbReference type="PDBsum" id="6PUZ"/>
    <dbReference type="PDBsum" id="6V3K"/>
    <dbReference type="PDBsum" id="6VOY"/>
    <dbReference type="AlphaFoldDB" id="P39476"/>
    <dbReference type="BMRB" id="P39476"/>
    <dbReference type="SMR" id="P39476"/>
    <dbReference type="STRING" id="273057.SSO10610"/>
    <dbReference type="iPTMnet" id="P39476"/>
    <dbReference type="PaxDb" id="273057-SSO10610"/>
    <dbReference type="EnsemblBacteria" id="AAK42679">
        <property type="protein sequence ID" value="AAK42679"/>
        <property type="gene ID" value="SSO10610"/>
    </dbReference>
    <dbReference type="GeneID" id="1454006"/>
    <dbReference type="GeneID" id="44128276"/>
    <dbReference type="KEGG" id="sso:SSO10610"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2631"/>
    <dbReference type="eggNOG" id="arCOG05888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2929990_0_0_2"/>
    <dbReference type="InParanoid" id="P39476"/>
    <dbReference type="BRENDA" id="1.8.99.B1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="P39476"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003212">
        <property type="entry name" value="DNA-bd_7a-e_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045555">
        <property type="entry name" value="Sul7d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02294">
        <property type="entry name" value="7kD_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036912">
        <property type="entry name" value="Sac7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed" evidence="3 5 7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000213078" description="DNA-binding protein 7d">
        <location>
            <begin position="2"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine" evidence="5 8">
        <location>
            <position position="5"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine" evidence="5 8">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine" evidence="5 8">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine" evidence="5 8">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine" evidence="5 8">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="3"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="17"/>
            <end position="19"/>
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    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="20"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="29"/>
            <end position="35"/>
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    <feature type="helix" evidence="11">
        <location>
            <begin position="37"/>
            <end position="39"/>
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    <feature type="strand" evidence="11">
        <location>
            <begin position="41"/>
            <end position="48"/>
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    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="53"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P61990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11709558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="1592111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="27853299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="3132977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="7634092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="7875327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="8">
        <source>
            <dbReference type="PubMed" id="9720912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="9">
        <source>
            <dbReference type="PubMed" id="3132977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="10"/>
    <evidence type="ECO:0007829" key="11">
        <source>
            <dbReference type="PDB" id="1C8C"/>
        </source>
    </evidence>
    <sequence length="64" mass="7280" checksum="0E4BBA5027B944DD" modified="2007-01-23" version="2">MATVKFKYKGEEKEVDISKIKKVWRVGKMISFTYDEGGGKTGRGAVSEKDAPKELLQMLEKQKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-04-20" modified="2026-01-28" version="148" xmlns="https://uniprot.org/uniprot">
    <accession>P95867</accession>
    <name>TREZ_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Malto-oligosyltrehalose trehalohydrolase</fullName>
            <shortName>MTHase</shortName>
            <ecNumber evidence="3">3.2.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>4-alpha-D-((1-&gt;4)-alpha-D-glucano)trehalose trehalohydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Maltooligosyl trehalose trehalohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">treZ</name>
        <name type="ordered locus">SSO2093</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="J. Agric. Food Chem." volume="56" first="5628" last="5633">
            <title>Identification of the essential catalytic residues and selectivity-related residues of maltooligosyltrehalose trehalohydrolase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092.</title>
            <authorList>
                <person name="Fang T.Y."/>
                <person name="Tseng W.C."/>
                <person name="Shih T.Y."/>
                <person name="Wang M.Y."/>
            </authorList>
            <dbReference type="PubMed" id="18563901"/>
            <dbReference type="DOI" id="10.1021/jf073320b"/>
        </citation>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF TRP-218; ASP-255; ALA-259; GLU-286; TYR-328; PHE-355; ARG-356 AND ASP-380</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>hydrolysis of (1-&gt;4)-alpha-D-glucosidic linkage in 4-alpha-D-[(1-&gt;4)-alpha-D-glucanosyl]n trehalose to yield trehalose and (1-&gt;4)-alpha-D-glucan.</text>
            <dbReference type="EC" id="3.2.1.141"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">7.22 mM for maltotriosyltrehalose</KM>
            <KM evidence="3">5.66 mM for maltotetraosyltrehalose</KM>
            <KM evidence="3">5.89 mM for maltopentaosyltrehalose</KM>
            <KM evidence="3">5.86 mM for maltopentaose</KM>
            <KM evidence="3">5.63 mM for maltohexaose</KM>
            <KM evidence="3">2.63 mM for maltoheptaose</KM>
        </kinetics>
        <phDependence>
            <text evidence="3">Optimum pH is 5.0.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="3">Optimum temperature is 85 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="5">Glycan biosynthesis; trehalose biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.141" evidence="3"/>
    <dbReference type="EMBL" id="Y08256">
        <property type="protein sequence ID" value="CAA69503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73087">
        <property type="entry name" value="S73087"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989788.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95867"/>
    <dbReference type="SMR" id="P95867"/>
    <dbReference type="FunCoup" id="P95867">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2093"/>
    <dbReference type="CAZy" id="CBM48">
        <property type="family name" value="Carbohydrate-Binding Module Family 48"/>
    </dbReference>
    <dbReference type="CAZy" id="GH13">
        <property type="family name" value="Glycoside Hydrolase Family 13"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2093"/>
    <dbReference type="EnsemblBacteria" id="AAK42272">
        <property type="protein sequence ID" value="AAK42272"/>
        <property type="gene ID" value="SSO2093"/>
    </dbReference>
    <dbReference type="GeneID" id="1453599"/>
    <dbReference type="GeneID" id="44130795"/>
    <dbReference type="KEGG" id="sso:SSO2093"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2171"/>
    <dbReference type="eggNOG" id="arCOG02951">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020726_2_0_2"/>
    <dbReference type="InParanoid" id="P95867"/>
    <dbReference type="PhylomeDB" id="P95867"/>
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        <property type="organism ID" value="6163"/>
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    <dbReference type="UniPathway" id="UPA00299"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033942">
        <property type="term" value="F:4-alpha-D-(1-&gt;4)-alpha-D-glucanotrehalose trehalohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11325">
        <property type="entry name" value="AmyAc_GTHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02853">
        <property type="entry name" value="E_set_MTHase_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.760">
        <property type="entry name" value="E-set domains of sugar-utilizing enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015156">
        <property type="entry name" value="Maltooligo_trehalose_arc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012768">
        <property type="entry name" value="Trehalose_TreZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044901">
        <property type="entry name" value="Trehalose_TreZ_E-set_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02402">
        <property type="entry name" value="trehalose_TreZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43002">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09071">
        <property type="entry name" value="Alpha-amyl_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006337">
        <property type="entry name" value="Trehalose_TreZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0326">Glycosidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000393752" description="Malto-oligosyltrehalose trehalohydrolase">
        <location>
            <begin position="1"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="253"/>
            <end position="258"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="311"/>
            <end position="315"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="379"/>
            <end position="384"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="5">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="15-fold and 150-fold reduction in specific activity, respectively." evidence="3">
        <original>W</original>
        <variation>F</variation>
        <variation>A</variation>
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="660-fold reduction in specific activity." evidence="3">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Insignificant change in specific activity." evidence="3">
        <original>A</original>
        <variation>S</variation>
        <location>
            <position position="259"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="1100-fold reduction in specific activity." evidence="3">
        <original>E</original>
        <variation>A</variation>
        <location>
            <position position="286"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="2-fold reduction in specific activity." evidence="3">
        <original>Y</original>
        <variation>F</variation>
        <location>
            <position position="328"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Very small decrease in specific activity." evidence="3">
        <original>F</original>
        <variation>Y</variation>
        <location>
            <position position="355"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="2-fold reduction in specific activity." evidence="3">
        <original>R</original>
        <variation>K</variation>
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="8140-fold reduction in specific activity." evidence="3">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q55088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="18563901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="18563901"/>
        </source>
    </evidence>
    <sequence length="561" mass="64371" checksum="B00E403020F6B242" modified="1997-05-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>P95993</accession>
    <name>RS2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Small ribosomal subunit protein uS2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>30S ribosomal protein S2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps2</name>
        <name evidence="7" type="synonym">rps2AB</name>
        <name type="ordered locus">SSO0067</name>
        <name evidence="5" type="ORF">C05004</name>
        <name type="ORF">C05_043</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="8 9 10" key="3">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Located near the mRNA exit channel, it binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the 30S small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75421">
        <property type="entry name" value="S75421"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988874.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="B=1-231"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="B=1-231"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="B=1-231"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="B=1-231"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="B=1-231"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="B=1-231"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="B=1-231"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="B=1-231"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="P95993"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="P95993"/>
    <dbReference type="FunCoup" id="P95993">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0067"/>
    <dbReference type="PaxDb" id="273057-SSO0067"/>
    <dbReference type="EnsemblBacteria" id="AAK40428">
        <property type="protein sequence ID" value="AAK40428"/>
        <property type="gene ID" value="SSO0067"/>
    </dbReference>
    <dbReference type="GeneID" id="1455319"/>
    <dbReference type="KEGG" id="sso:SSO0067"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.67"/>
    <dbReference type="eggNOG" id="arCOG04245">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058171_3_0_2"/>
    <dbReference type="InParanoid" id="P95993"/>
    <dbReference type="PhylomeDB" id="P95993"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000030">
        <property type="entry name" value="30S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_A">
        <property type="entry name" value="Ribosomal_uS2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023454">
        <property type="entry name" value="Ribosomal_uS2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_uS2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000134334" description="Small ribosomal subunit protein uS2">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="8899719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <evidence type="ECO:0000312" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="10">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="231" mass="26007" checksum="3589A0CBE36AC59D" modified="1997-05-01" version="1">MKVTNLSEKEERGGELTEAEKEELRKSEKGAIIELLVPVDTYLSAGVHIGTHSCTKYMESFVYRVRAEGLYVLDVRKIDERLRIAAKFLSRYDPQDIIVVASRPYAYRPVQKFAEVVGSRALVGRIIPGTFTNPYLSTYIEPKVLLVSDPRTDTQAIKEAAKVGIPIVAFADTDAKIDYIDLIIPANNKGRKSLALLYWALARQILRERRVIPPDGDLAVPVSEFEMRLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX45</accession>
    <name>Q7LX45_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74067">
        <property type="entry name" value="S74067"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992153.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX45"/>
    <dbReference type="STRING" id="273057.SSO2160"/>
    <dbReference type="PaxDb" id="273057-SSO2160"/>
    <dbReference type="EnsemblBacteria" id="AAK42336">
        <property type="protein sequence ID" value="AAK42336"/>
        <property type="gene ID" value="SSO2160"/>
    </dbReference>
    <dbReference type="GeneID" id="1453663"/>
    <dbReference type="KEGG" id="sso:SSO2160"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2253"/>
    <dbReference type="eggNOG" id="arCOG04479">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162441_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX45"/>
    <dbReference type="PhylomeDB" id="Q7LX45"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038767">
        <property type="entry name" value="PF0610-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049159">
        <property type="entry name" value="PF0610-like_wHTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057022">
        <property type="entry name" value="PF0610-like_Zn_ribbon_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40663">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40663:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21476">
        <property type="entry name" value="PF0610-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23470">
        <property type="entry name" value="Zn_ribbon_PF0610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PF0610-like winged HTH N-terminal" evidence="1">
        <location>
            <begin position="7"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="PF0610-like rubredoxin-like zinc beta-ribbon C-terminal" evidence="2">
        <location>
            <begin position="62"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="12002" checksum="88E281C0F8DCD8D5" modified="2011-05-31" version="1">MSTDFLTTREKIFLLLFYSDEPLSAREIMRRLDVRKEKEVYDHLEHLARSSKRKGYMLIIIPAKCKSCGYVFNSDKIKKPSRCPICKSEKIEMPKFLIRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX70</accession>
    <name>Q7LX70_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Pyruvate synthase delta chain (Pyruvic-ferredoxin oxidoreductase delta chain) (PorD-like)</fullName>
            <ecNumber evidence="10">1.2.7.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">porD-like</name>
        <name evidence="10" type="ordered locus">SSO2128</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.2.7.1" evidence="10"/>
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        <property type="protein sequence ID" value="AAK42307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="S74040"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX70"/>
    <dbReference type="FunCoup" id="Q7LX70">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2128"/>
    <dbReference type="PaxDb" id="273057-SSO2128"/>
    <dbReference type="EnsemblBacteria" id="AAK42307">
        <property type="protein sequence ID" value="AAK42307"/>
        <property type="gene ID" value="SSO2128"/>
    </dbReference>
    <dbReference type="GeneID" id="1453634"/>
    <dbReference type="KEGG" id="sso:SSO2128"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2220"/>
    <dbReference type="eggNOG" id="arCOG01604">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_764230_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX70"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011898">
        <property type="entry name" value="PorD_KorD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02179">
        <property type="entry name" value="PorD_KorD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724:SF1">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="261"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="291"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="363" mass="41755" checksum="88156FBB0CB0EADB" modified="2011-05-31" version="1">MGVKDEVKYVEIVYRGIFQKRLAKYIAEGIVYTAREMGRPALSFGRYGDSPERNGVPAKYYVGIGDGVNEEDLIGYSTRVEPDLVDVIIVLDDTLLKGVESWAWQGVQPINLKLKSNGTMLVTSTKRINELLKMIPKKDFNWTLGVIKTEPSFSGLWAFKDDLTMEKVWGGLAKLRPDIIDLDHLLKYVTKKQDANKRVSAVREAYSSVDYRTVMKGEGIDFVYNPPRLLTWQEMLEGTVIPAVPRGKRNELFKRGTTKFERPTVDFDTCIKCKLCWVYCPDECFDETPDGYYDIAYDYCVGCGICAEVCPVKDCIVMVDESMFTDYRRPYEMWKEDKAKYKEWLKTVRQARKERVYVPGLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX77</accession>
    <name>Q7LX77_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transglutaminase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="S74031"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990152.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX77"/>
    <dbReference type="SMR" id="Q7LX77"/>
    <dbReference type="STRING" id="273057.SSO2119"/>
    <dbReference type="PaxDb" id="273057-SSO2119"/>
    <dbReference type="EnsemblBacteria" id="AAK42298">
        <property type="protein sequence ID" value="AAK42298"/>
        <property type="gene ID" value="SSO2119"/>
    </dbReference>
    <dbReference type="GeneID" id="1453625"/>
    <dbReference type="KEGG" id="sso:SSO2119"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2208"/>
    <dbReference type="eggNOG" id="arCOG02165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1140614_0_0_2"/>
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    <dbReference type="PhylomeDB" id="Q7LX77"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
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        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
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        <property type="entry name" value="BLR5614 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33490:SF6">
        <property type="entry name" value="SLL1049 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="1">
        <location>
            <begin position="144"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="243" mass="28157" checksum="784F6D2E5728B4D2" modified="2011-05-31" version="1">MTRYKLSAEVSFSKPSEIMGRLYILPYRDNAQKVLHFNIDHPSFCKVSINQDRFRNEHLDFKCNPSDDFSIIISYEVEVNSFSKSENEEEVFLSSSKYVNVDKFKMINVRYFSFDDLIDEVISMVRDSIRYDKNNKSVKSAFGSLIMGSGVCVNYSHVAIGILRALGIPARYVIGLIPFSTKEAHAWIEVKIGDVWFPVDPTNGIKGMQYLKWAIGRDDNDVRSKIWYSKPVSFDFSFDWNFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXC0</accession>
    <name>Q7LXC0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0787</name>
        <name evidence="3" type="ordered locus">SSO1466</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90228">
        <property type="entry name" value="F90228"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923080.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXC0"/>
    <dbReference type="STRING" id="273057.SSO0787"/>
    <dbReference type="PaxDb" id="273057-SSO0787"/>
    <dbReference type="EnsemblBacteria" id="AAK41085">
        <property type="protein sequence ID" value="AAK41085"/>
        <property type="gene ID" value="SSO0787"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41694">
        <property type="protein sequence ID" value="AAK41694"/>
        <property type="gene ID" value="SSO1466"/>
    </dbReference>
    <dbReference type="GeneID" id="1455052"/>
    <dbReference type="KEGG" id="sso:SSO0787"/>
    <dbReference type="KEGG" id="sso:SSO1466"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1499"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="80"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37383" checksum="34590B407E4B4CC8" modified="2011-05-31" version="1">MQTIQVSKTELKSLAINLATNNINVISQDLDPEIVKAAPSLLTGNRGKYYLKVVRRGEKVISKGQRTFKFYPIYREVKGEINVVAVDETGLTVGEKEQEKAEGFLLYNWKRKGVKMRSLDLVYPLRLPLLVEVADLRSDSPSQFLLRSVREVSQYMEIDYVVADAGFLNLGVIKEMPVKTIVRGKSNLKGFKELSNVPLVEKRYEVKDKVYVAYRVLKFEGLYYYDVVYVKGKPRHFMFVTNFEGDPYELAELYRLRWQVEEGFKVRKARIRYVRKLSNKIFLFLYYTVLDSAWNLVNHLLFNFKSTCKKVLSFDSFVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXF6</accession>
    <name>Q7LXF6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase FkbM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90232">
        <property type="entry name" value="F90232"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923112.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXF6"/>
    <dbReference type="STRING" id="273057.SSO0821"/>
    <dbReference type="PaxDb" id="273057-SSO0821"/>
    <dbReference type="EnsemblBacteria" id="AAK41117">
        <property type="protein sequence ID" value="AAK41117"/>
        <property type="gene ID" value="SSO0821"/>
    </dbReference>
    <dbReference type="GeneID" id="1455084"/>
    <dbReference type="KEGG" id="sso:SSO0821"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.833"/>
    <dbReference type="eggNOG" id="arCOG01400">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080078_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXF6"/>
    <dbReference type="PhylomeDB" id="Q7LXF6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052514">
        <property type="entry name" value="SAM-dependent_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203">
        <property type="entry name" value="METHYLTRANSFERASE, FKBM FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203:SF15">
        <property type="entry name" value="SLL1173 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase FkbM" evidence="1">
        <location>
            <begin position="133"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="307" mass="34695" checksum="EC575A1965E3EEB5" modified="2011-05-31" version="1">MANYASFIEVLISNFNRYPYTLFPLYAKAYKNPLIVALKSLLRHYPFVVELKNGEKVLVTGKGYKIASYYAFLYLARKKGKKINIIDIDENTIKIIYDTNEIILKDYTKGDIIGVYIKEEYQDVNVKDKTVIDCGAAIGDSAIYFSLKGAKRVIAIEAYPKIAKIAEENVKLNNFKNIQIVNAACGTDGIVTVNPRLGGGPTPLMSSNNGYTVPSFSLQGLIKNFQVEKGSVLKLDCEGCEYEFLLNADSSTLEYFDQIIMEYHYGYSHLKRKLEKAGFKVKVTKPELWNNQYKKGLILGKLYASRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2023-11-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXI0</accession>
    <name>Q7LXI0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0793</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90229">
        <property type="entry name" value="D90229"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXI0"/>
    <dbReference type="PaxDb" id="273057-SSO0793"/>
    <dbReference type="EnsemblBacteria" id="AAK41091">
        <property type="protein sequence ID" value="AAK41091"/>
        <property type="gene ID" value="SSO0793"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0793"/>
    <dbReference type="HOGENOM" id="CLU_1891530_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXI0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="134" mass="15669" checksum="043A7F887FFC96C4" modified="2011-05-31" version="1">MCERFMDSIDKCRIECKFVENKDKREFLAERLNNLTRYLERICRALNDVCKNSATVSCYQCFDEVNKAYNTAVCEDSCKGKSRLSFCFLLSEGLILNVIAEDEGGCRTIITFYPINRDRERTIEKKCDKIFNQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXI1</accession>
    <name>Q7LXI1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0792</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90229">
        <property type="entry name" value="C90229"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923085.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXI1"/>
    <dbReference type="PaxDb" id="273057-SSO0792"/>
    <dbReference type="EnsemblBacteria" id="AAK41090">
        <property type="protein sequence ID" value="AAK41090"/>
        <property type="gene ID" value="SSO0792"/>
    </dbReference>
    <dbReference type="GeneID" id="1455057"/>
    <dbReference type="KEGG" id="sso:SSO0792"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.799"/>
    <dbReference type="eggNOG" id="arCOG07288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157023_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXI1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="139" mass="15438" checksum="5FAEC6A1A986A6E6" modified="2011-05-31" version="1">MSEAVYTFDVTLIKPTVFKFTIGEEIVTVMLYLIPQAVIISEKEEEGKKLPSVVSMGTLTVVSNKPKMGELCSADKFSRHTPFIPKIEIVSNGVTEVKVNYMKIKISAKVTNVNVYPDLRDSLGNPCVNVSWIQLISVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXK0</accession>
    <name>Q7LXK0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamyl-tRNA amidotransferase, subunit A (GatA-1)</fullName>
            <ecNumber evidence="2">6.3.5.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gatA-1</name>
        <name evidence="2" type="ordered locus">SSO0765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.3.5.-" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90225">
        <property type="entry name" value="G90225"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991353.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXK0"/>
    <dbReference type="FunCoup" id="Q7LXK0">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0765"/>
    <dbReference type="PaxDb" id="273057-SSO0765"/>
    <dbReference type="EnsemblBacteria" id="AAK41062">
        <property type="protein sequence ID" value="AAK41062"/>
        <property type="gene ID" value="SSO0765"/>
    </dbReference>
    <dbReference type="GeneID" id="1455029"/>
    <dbReference type="KEGG" id="sso:SSO0765"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.764"/>
    <dbReference type="eggNOG" id="arCOG01717">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_3_2"/>
    <dbReference type="InParanoid" id="Q7LXK0"/>
    <dbReference type="PhylomeDB" id="Q7LXK0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF67">
        <property type="entry name" value="AMIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="20"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="398" mass="44053" checksum="72F15D39B48536F3" modified="2011-05-31" version="1">MKLEELNSKYNAFITLHTIEEGNDKGKLKDVTFGIKDVILTKDVRTTAGSRVLENYVPQRNAYVVDAILREGGKIIGKTNTHEFALGATNTSSIGGPARNPVDPERISGGSSGGSAVAVKLDMVDVGIGTDTGGSVRIPSSLCGVIGFKPTTGLIPNDGVIPFSWSLDTVGIIVKDNIRLLRRVFDVVLPNEKRKVEIAKLRTRPRLGLFLFDDMEVSRILLKDIYAKLSSHFDIVEVDLPLLRQYGSKTRRTISLAEAASYHKDWISEYSDKYFKDTYTLLLDGMKISATDYIDALRHRRVLIEEYVKVFKNIDFILSPTTKIVAPRISDVISNPLQFREYLIANTELFNVVGAPSISVPFSTLNDLPAGLMISGELYKDGDLLEVAEYILNIVGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXT0</accession>
    <name>Q7LXT0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0591</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90205">
        <property type="entry name" value="H90205"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991109.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXT0"/>
    <dbReference type="STRING" id="273057.SSO0591"/>
    <dbReference type="PaxDb" id="273057-SSO0591"/>
    <dbReference type="EnsemblBacteria" id="AAK40903">
        <property type="protein sequence ID" value="AAK40903"/>
        <property type="gene ID" value="SSO0591"/>
    </dbReference>
    <dbReference type="GeneID" id="1454870"/>
    <dbReference type="KEGG" id="sso:SSO0591"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.599"/>
    <dbReference type="eggNOG" id="arCOG05993">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117490_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXT0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="199" mass="23417" checksum="2FDB229D715EF81D" modified="2011-05-31" version="1">MNLLDYLMSLRNRKILLQSYHINAKIVLIYNITQRLYKENISVCIINYEKFLKLSIREIDQRCVEGSYVIVFEAEEASDLPIRYNLVTSFVKINKDFDDILRIDKISTNLYKAQNNAGDLFIFSVINGKVIDRKLRPIHEDIINFLREYGGEVEIRDLVNIISKKYETNRDNVRVELAFLKELGIIEVKDRKVILTQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXV8</accession>
    <name>Q7LXV8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90200">
        <property type="entry name" value="D90200"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991033.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXV8"/>
    <dbReference type="PaxDb" id="273057-SSO0540"/>
    <dbReference type="EnsemblBacteria" id="AAK40859">
        <property type="protein sequence ID" value="AAK40859"/>
        <property type="gene ID" value="SSO0540"/>
    </dbReference>
    <dbReference type="GeneID" id="1454826"/>
    <dbReference type="KEGG" id="sso:SSO0540"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.539"/>
    <dbReference type="eggNOG" id="arCOG07284">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150445_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXV8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="143" mass="16630" checksum="4805F97FB8A336E5" modified="2011-05-31" version="1">MSREIEVPFVKVNDTYLPLLKVEMECPKLGSEYLVYALPDTGSRFSIIRNDTFLKCFDESSLKNRLVDKVIISNLLTTKERYNIKFHFVELNETLQIPVTSLDFVNLGEGIYPSLILGREDFFSRIMICFERNVRLIIKANDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXY6</accession>
    <name>Q7LXY6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75381">
        <property type="entry name" value="S75381"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988927.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXY6"/>
    <dbReference type="STRING" id="273057.SSO0108"/>
    <dbReference type="PaxDb" id="273057-SSO0108"/>
    <dbReference type="EnsemblBacteria" id="AAK40463">
        <property type="protein sequence ID" value="AAK40463"/>
        <property type="gene ID" value="SSO0108"/>
    </dbReference>
    <dbReference type="GeneID" id="1453291"/>
    <dbReference type="KEGG" id="sso:SSO0108"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.104"/>
    <dbReference type="eggNOG" id="arCOG05988">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1811488_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXY6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="130" mass="14805" checksum="BB051A919F6EC374" modified="2011-05-31" version="1">MSSVTEDNLKPNIVLLSTSDLEQEIRQLTEELKNVKDNNNEEHKKLYALVDNITRTLNWINIAKSQGIWKSKTCKHAINFVCQAWNISDESKLGIPSDVIVINDDGTKRVVVSKFSEICIVCPLYEARRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-10-02" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TV9</accession>
    <name>Q97TV9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">First ORF in transposon ISC1048</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1705</name>
        <name evidence="3" type="ordered locus">SSO1794</name>
        <name evidence="4" type="ordered locus">SSO2930</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90331">
        <property type="entry name" value="H90331"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TV9"/>
    <dbReference type="STRING" id="273057.SSO1705"/>
    <dbReference type="PaxDb" id="273057-SSO1705"/>
    <dbReference type="EnsemblBacteria" id="AAK41911">
        <property type="protein sequence ID" value="AAK41911"/>
        <property type="gene ID" value="SSO1705"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41987">
        <property type="protein sequence ID" value="AAK41987"/>
        <property type="gene ID" value="SSO1794"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK43036">
        <property type="protein sequence ID" value="AAK43036"/>
        <property type="gene ID" value="SSO2930"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1705"/>
    <dbReference type="KEGG" id="sso:SSO1794"/>
    <dbReference type="KEGG" id="sso:SSO2930"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1755"/>
    <dbReference type="eggNOG" id="arCOG02127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124929_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025959">
        <property type="entry name" value="Winged_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13551">
        <property type="entry name" value="HTH_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13592">
        <property type="entry name" value="HTH_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Winged helix-turn helix" evidence="1">
        <location>
            <begin position="94"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="164" mass="19374" checksum="62A8D03AD47C33A9" modified="2001-10-01" version="1">MQDLVQAYKEEKNARIKERILAVKLHVVDGKSEKEVSKMLNKGYSTIKLWIGKYKKEGLDGLKDKPRSGRPRKVEEEKIKQILEDKPQKYGIQQEYWTMKTLKIALQEQGIEYKKSRLYELVHELGYNLVKPRPTNIQAEKDKWEDFKKNKEIGKKGTILSRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TW7</accession>
    <name>Q97TW7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Partial transposase ISC1359</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1703</name>
        <name evidence="4" type="ordered locus">SSO1796</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90331">
        <property type="entry name" value="F90331"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TW7"/>
    <dbReference type="PaxDb" id="273057-SSO1703"/>
    <dbReference type="EnsemblBacteria" id="AAK41909">
        <property type="protein sequence ID" value="AAK41909"/>
        <property type="gene ID" value="SSO1703"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41989">
        <property type="protein sequence ID" value="AAK41989"/>
        <property type="gene ID" value="SSO1796"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1703"/>
    <dbReference type="KEGG" id="sso:SSO1796"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067987_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="16"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="251" mass="29387" checksum="0E6AB3609246ED7B" modified="2001-10-01" version="1">MVREYPKTTNRIKPTKGTSWGLVQAAIFLLGRTRSFLDVIPVTVKNVAEGFKAVMEVIVKELEEDKLRLVMVFADKEFAVNEVIRYLLELGLDFVISAKVQMYKKYKGMLQDVDVSFGGVRYTGFLCVRYGSGAYLIILRKEDGKIIAFLVRREMDLYDAIVLAEMYRERWGIENAFRSLEEFRIRTRTCDVRKELVLVLLSYLLLNVWFLIRSWRKVKLWEFSVSLSNLLDREVRVEQGRAFREVKTPFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TX5</accession>
    <name>Q97TX5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Gamma-glutamyltranspeptidase, hypothetical</fullName>
            <ecNumber evidence="1">2.3.2.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3216</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.2.2" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90507">
        <property type="entry name" value="B90507"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992739.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TX5"/>
    <dbReference type="FunCoup" id="Q97TX5">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3216"/>
    <dbReference type="PaxDb" id="273057-SSO3216"/>
    <dbReference type="EnsemblBacteria" id="AAK43313">
        <property type="protein sequence ID" value="AAK43313"/>
        <property type="gene ID" value="SSO3216"/>
    </dbReference>
    <dbReference type="GeneID" id="1453232"/>
    <dbReference type="KEGG" id="sso:SSO3216"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3319"/>
    <dbReference type="eggNOG" id="arCOG04053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014813_3_3_2"/>
    <dbReference type="InParanoid" id="Q97TX5"/>
    <dbReference type="PhylomeDB" id="Q97TX5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052896">
        <property type="entry name" value="GGT-like_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43881">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSPEPTIDASE (AFU_ORTHOLOGUE AFUA_4G13580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43881:SF1">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSPEPTIDASE (AFU_ORTHOLOGUE AFUA_4G13580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="478" mass="53112" checksum="2580CBF11675D028" modified="2001-10-01" version="1">MPSAVGKKVVTTQNYIASYVGAKVLEEGGNAFDAAIAISATLSVVIPHTSGLGGDGFLLAKTPEGLIAYNASGWAPKELKVDKINSDRGPLTVVVPGLVDLWEFIYENYASKSLHELLIPAISLATNGFMVGRGLHHAIISSFKLSDDWNRVYGSKRFGDEIKLRGIAKVLKEISKDPRSFYEGKIAEELTKGLRERGVPVSYEDFSEFHGEIINPIKMKYKDFMLYELPPNTQGLTTLELLKMIEVTEINKLPFNDVRRVNDHVRLSALAYADRNNYIADPRFVNVPVEMLLSEKYIANKIAEYGFEVKVNVTGDTTFFAVADGENEIGFIQSLFYPFGSGIVVNDIPFNNRGAGFSEGNNKPEPRKRPLHTLSILMAEKDDERIFIGCAGGDLRPQIHAEVFEYYADYNMEIDEAVQAPRFMYLGNKVIAEKRLGVPATQTDYYSPEVGIVQALKYKKGRYIGVADIRSEGIVLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U53</accession>
    <name>Q97U53_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TsaA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO3170</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA methyltransferase O family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90501">
        <property type="entry name" value="F90501"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989980.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U53"/>
    <dbReference type="STRING" id="273057.SSO3170"/>
    <dbReference type="PaxDb" id="273057-SSO3170"/>
    <dbReference type="EnsemblBacteria" id="AAK43269">
        <property type="protein sequence ID" value="AAK43269"/>
        <property type="gene ID" value="SSO3170"/>
    </dbReference>
    <dbReference type="GeneID" id="1453188"/>
    <dbReference type="KEGG" id="sso:SSO3170"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3274"/>
    <dbReference type="eggNOG" id="arCOG00761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013458_2_1_2"/>
    <dbReference type="InParanoid" id="Q97U53"/>
    <dbReference type="PhylomeDB" id="Q97U53"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09281">
        <property type="entry name" value="UPF0066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.70">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023370">
        <property type="entry name" value="TrmO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040372">
        <property type="entry name" value="YaeB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036413">
        <property type="entry name" value="YaeB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036414">
        <property type="entry name" value="YaeB_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00104">
        <property type="entry name" value="tRNA_TsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818:SF0">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01980">
        <property type="entry name" value="TrmO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118196">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51668">
        <property type="entry name" value="TSAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="TsaA-like" evidence="3">
        <location>
            <begin position="5"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="124" mass="13835" checksum="97969FAF97DD6341" modified="2001-10-01" version="1">MNACFKYIGFIKRNDNSTSRNAIIEIHVNKEYEEGLKGLEEFSHIIVIYHLHLASFDGRLLREKKGVTVGIFATRSQFRPNPIGISVAELIEIKGNIVLAKGINAFNNTPVLDIKPLSAVSDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U74</accession>
    <name>Q97U74_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Second ORF in partial transposon ISC1160</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO12067</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90498">
        <property type="entry name" value="H90498"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U74"/>
    <dbReference type="PaxDb" id="273057-SSO12067"/>
    <dbReference type="EnsemblBacteria" id="AAK43247">
        <property type="protein sequence ID" value="AAK43247"/>
        <property type="gene ID" value="SSO12067"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO12067"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3252"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2476184_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U74"/>
    <dbReference type="PhylomeDB" id="Q97U74"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025471">
        <property type="entry name" value="DUF4322"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14210">
        <property type="entry name" value="DUF4322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4322" evidence="3">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="87" mass="10271" checksum="BD682EADD7B77045" modified="2001-10-01" version="1">MIDFKGRKGKEQVKPSSQPWNDSIENKSKMFETSSQTVRNYAEKEEVAEKLLERVKQLSLEMLKAVRKVNISIDWTTIRWQTGREVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U85</accession>
    <name>Q97U85_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90497">
        <property type="entry name" value="E90497"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990918.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U85"/>
    <dbReference type="STRING" id="273057.SSO3138"/>
    <dbReference type="PaxDb" id="273057-SSO3138"/>
    <dbReference type="EnsemblBacteria" id="AAK43236">
        <property type="protein sequence ID" value="AAK43236"/>
        <property type="gene ID" value="SSO3138"/>
    </dbReference>
    <dbReference type="GeneID" id="1453155"/>
    <dbReference type="KEGG" id="sso:SSO3138"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3242"/>
    <dbReference type="eggNOG" id="arCOG06003">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1682780_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U85"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="166" mass="18350" checksum="76DB5C0A2BA50659" modified="2001-10-01" version="1">MRKAQSEYIGFIIAIIIIVLIVIPLFYILSNYSVPSAKQLDYVQVLKNQINGGGILIFFNSTYPKSSSSSVWANSTLIVLKGNANYTLTGVYYMKGGVIYNITSKVIPFIIANGQANPINGNLPQPLIYNFSLPGYVWDYPVVLQISGYNVTVFAQVYPNETAFTS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2014-05-14" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UA1</accession>
    <name>KGSDH_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>2,5-dioxopentanoate dehydrogenase</fullName>
            <shortName evidence="5">DopDH</shortName>
            <ecNumber evidence="4">1.2.1.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Aldehyde dehydrogenase T</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Alpha-ketoglutaric semialdehyde dehydrogenase AldhT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">dopDH</name>
        <name evidence="7" type="synonym">aldhT</name>
        <name type="ordered locus">SSO3117</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="J. Biol. Chem." volume="281" first="27378" last="27388">
            <title>Identification of the missing links in prokaryotic pentose oxidation pathways: evidence for enzyme recruitment.</title>
            <authorList>
                <person name="Brouns S.J."/>
                <person name="Walther J."/>
                <person name="Snijders A.P."/>
                <person name="van de Werken H.J."/>
                <person name="Willemen H.L."/>
                <person name="Worm P."/>
                <person name="de Vos M.G."/>
                <person name="Andersson A."/>
                <person name="Lundgren M."/>
                <person name="Mazon H.F."/>
                <person name="van den Heuvel R.H."/>
                <person name="Nilsson P."/>
                <person name="Salmon L."/>
                <person name="de Vos W.M."/>
                <person name="Wright P.C."/>
                <person name="Bernander R."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="16849334"/>
            <dbReference type="DOI" id="10.1074/jbc.M605549200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>INDUCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">2,5-dioxopentanoate dehydrogenase involved in the degradation of pentoses such as D-arabinose or D-xylose, a major component of hemicelluloses such as xylan. Catalyzes the fifth reaction in the pentose utilization pathway through dehydratation of 2,5-dioxopentanoate into 2-oxoglutarate. Also shows dehydrogenase activity toward glycolaldehyde and DL-glyceraldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>2,5-dioxopentanoate + NADP(+) + H2O = 2-oxoglutarate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58136"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.26"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="4">Optimum pH is 7.8.</text>
        </phDependence>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="induction">
        <text evidence="4">Expression is highly induced during growth on D-arabinose. The promoter contains the conserved cis-regulatory element 5'-AACATGTT-3' ARA-box found in arabinose-induced genes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.26" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90495">
        <property type="entry name" value="E90495"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990943.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UA1"/>
    <dbReference type="SMR" id="Q97UA1"/>
    <dbReference type="FunCoup" id="Q97UA1">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3117"/>
    <dbReference type="PaxDb" id="273057-SSO3117"/>
    <dbReference type="EnsemblBacteria" id="AAK43220">
        <property type="protein sequence ID" value="AAK43220"/>
        <property type="gene ID" value="SSO3117"/>
    </dbReference>
    <dbReference type="GeneID" id="1453139"/>
    <dbReference type="GeneID" id="44128838"/>
    <dbReference type="KEGG" id="sso:SSO3117"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3225"/>
    <dbReference type="eggNOG" id="arCOG01252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_1_2"/>
    <dbReference type="InParanoid" id="Q97UA1"/>
    <dbReference type="PhylomeDB" id="Q97UA1"/>
    <dbReference type="BioCyc" id="MetaCyc:MONOMER-13207"/>
    <dbReference type="BRENDA" id="1.2.1.22">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="1.2.1.26">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047533">
        <property type="term" value="F:2,5-dioxovalerate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019568">
        <property type="term" value="P:arabinose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07097">
        <property type="entry name" value="ALDH_KGSADH-YcbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000005">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000007">
        <property type="entry name" value="NAD/NADP-dependent betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0054">Arabinose catabolism</keyword>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000428928" description="2,5-dioxopentanoate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2 3">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2 3">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="148"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="172"/>
            <end position="175"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="225"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="16849334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="16849334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <evidence type="ECO:0000312" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43220.1"/>
        </source>
    </evidence>
    <sequence length="478" mass="52290" checksum="48D83AADC1B0686C" modified="2001-10-01" version="1">MKSYQGLADKWIKGSGEEYLDINPADKDHVLAKIRLYTKDDVKEAINKAVAKFDEWSRTPAPKRGSILLKAGELMEQEAQEFALLMTLEEGKTLKDSMFEVTRSYNLLKFYGALAFKISGKTLPSADPNTRIFTVKEPLGVVALITPWNFPLSIPVWKLAPALAAGNTAVIKPATKTPLMVAKLVEVLSKAGLPEGVVNLVVGKGSEVGDTIVSDDNIAAVSFTGSTEVGKRIYKLVGNKNRMTRIQLELGGKNALYVDKSADLTLAAELAVRGGFGLTGQSCTATSRLIINKDVYTQFKQRLLERVKKWRVGPGTEDVDMGPVVDEGQFKKDLEYIEYGKNVGAKLIYGGNIIPGKGYFLEPTIFEGVTSDMRLFKEEIFGPVLSVTEAKDLDEAIRLVNAVDYGHTAGIVASDIKAINEFVSRVEAGVIKVNKPTVGLELQAPFGGFKNSGATTWKEMGEDALEFYLKEKTVYEGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UB6</accession>
    <name>Q97UB6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO3101</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90493">
        <property type="entry name" value="F90493"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924134.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UB6"/>
    <dbReference type="PaxDb" id="273057-SSO3101"/>
    <dbReference type="EnsemblBacteria" id="AAK43205">
        <property type="protein sequence ID" value="AAK43205"/>
        <property type="gene ID" value="SSO3101"/>
    </dbReference>
    <dbReference type="GeneID" id="1453124"/>
    <dbReference type="KEGG" id="sso:SSO3101"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3208"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UB6"/>
    <dbReference type="PhylomeDB" id="Q97UB6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="80"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37410" checksum="D5CDD06947DDF20A" modified="2001-10-01" version="1">MQVYETGFKTELKSLASYFIVNNLNVISQELDTRIADAAPFLLTGNRGKEYLKVVREGEKVMSQGERSFKFYPHVKVEGETTIVAVDETAITVGEKESRKVEGFQLYNGKRKGVKLRSVDLVFPLRLSLLEEIADLRSDTPSQFLMRAVSEVAQHLKIDYVVADAGFLNLKVIKEMPVKTVVGGKTNLKGFKELSNVPLTEKKCEVNDRVYVAYRVLKYNDLYYYEVIYVKEKPRHFIFVTNFNGDPYKLAELYRLRWQSEEGFKVRKARIRYVRKLTNKIFLYLYYTVLDSVWNLVNYLLFNFKSTKRKPLSFNSFVRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UD7</accession>
    <name>Q97UD7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH bat-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99491">
        <property type="entry name" value="A99491"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989098.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UD7"/>
    <dbReference type="PaxDb" id="273057-SSO3080"/>
    <dbReference type="DNASU" id="1453103"/>
    <dbReference type="EnsemblBacteria" id="AAK43184">
        <property type="protein sequence ID" value="AAK43184"/>
        <property type="gene ID" value="SSO3080"/>
    </dbReference>
    <dbReference type="GeneID" id="84062562"/>
    <dbReference type="KEGG" id="sso:SSO3080"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3186"/>
    <dbReference type="eggNOG" id="arCOG02272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104910_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UD7"/>
    <dbReference type="PhylomeDB" id="Q97UD7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH bat-type" evidence="1">
        <location>
            <begin position="162"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="221" mass="25793" checksum="B1C9629172F31CF5" modified="2001-10-01" version="1">MIRSYNVVLSHEDWTLLTDKYDEEDLRVLMKIRTPSFEKMEENIIAEAYVRDPLILNELINIIKSNNRVTRVKIIENVRTKNELRALILLSAKLRGGISELLMNKGAYYISERISNGLEKWSLVIDDKIFREIILPTLKEYTYSLKIIEKDDGNLVSVKNLLTSKEFEIIYKAYKMGYFDWPKKVDLKELSEELGISKAATLQALRRAMGKLIRNYVDNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UE4</accession>
    <name>Q97UE4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO11909</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90490">
        <property type="entry name" value="B90490"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989089.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UE4"/>
    <dbReference type="STRING" id="273057.SSO11909"/>
    <dbReference type="PaxDb" id="273057-SSO11909"/>
    <dbReference type="EnsemblBacteria" id="AAK43177">
        <property type="protein sequence ID" value="AAK43177"/>
        <property type="gene ID" value="SSO11909"/>
    </dbReference>
    <dbReference type="GeneID" id="25392528"/>
    <dbReference type="KEGG" id="sso:SSO11909"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3179"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208838_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UE4"/>
    <dbReference type="PhylomeDB" id="Q97UE4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="60" mass="7025" checksum="C5EFCCEB9EEA628F" modified="2001-10-01" version="1">MRRAFYLAALTAIKVNPVIKRFYEEHKGRLRGKKLIVACARKLAVIIWAVLYYRPFDASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UG2</accession>
    <name>Q97UG2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha-L-fucosidase</fullName>
            <ecNumber evidence="3">3.2.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fucA1</name>
        <name evidence="8" type="ordered locus">SSO3060</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 29 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.51" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90487">
        <property type="entry name" value="H90487"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UG2"/>
    <dbReference type="SMR" id="Q97UG2"/>
    <dbReference type="FunCoup" id="Q97UG2">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3060"/>
    <dbReference type="CAZy" id="GH29">
        <property type="family name" value="Glycoside Hydrolase Family 29"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3060"/>
    <dbReference type="EnsemblBacteria" id="AAK43159">
        <property type="protein sequence ID" value="AAK43159"/>
        <property type="gene ID" value="SSO3060"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3060"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3157"/>
    <dbReference type="eggNOG" id="arCOG07338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002934_6_1_2"/>
    <dbReference type="InParanoid" id="Q97UG2"/>
    <dbReference type="PhylomeDB" id="Q97UG2"/>
    <dbReference type="SABIO-RK" id="Q97UG2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004560">
        <property type="term" value="F:alpha-L-fucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016139">
        <property type="term" value="P:glycoside catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016286">
        <property type="entry name" value="FUC_metazoa-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000933">
        <property type="entry name" value="Glyco_hydro_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057739">
        <property type="entry name" value="Glyco_hydro_29_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10030">
        <property type="entry name" value="ALPHA-L-FUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10030:SF37">
        <property type="entry name" value="ALPHA-L-FUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01120">
        <property type="entry name" value="Alpha_L_fucos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001092">
        <property type="entry name" value="Alpha-L-fucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00812">
        <property type="entry name" value="Alpha_L_fucos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycoside hydrolase family 29 N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK43159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="403" mass="46554" checksum="2168A20042E9B473" modified="2001-10-01" version="1">MFTGENWDPYEWAKVFKKSGAKFVVLVAEHHDGFALWESNYTRWCATKIGPKRDIVRELKEAVEGQGLIFGISYHRAEHWWFFDQGMKIESDVKDPRYLDLYGPAQSASLNPRDPPSLDNVQPNDEFLMDWLLRIVEAVEKYRPWLVYFDWWIANPSFQPYLKAFASYYYNRSYKWGIEPVIIYKQGAFGEGTAIPDLAERGTIKNVYPSTWLADTSIDYKSWGYIKDAEYKLPSVILSHLGDVVSKNGVFLLNIGPKADGTIPEEAKRILLDVGDWLNVNGEAIFGSKPWRVYGEGPSGINEGGFFTERKITLGYQDVRYTVKDYYPRQRHIYAILFGKPKEITLRSFMKNLKLIEEAVIVDVSRLDGKGKLEWSLSDEGLKIKIEEVIRAPLVIRVILDYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UG6</accession>
    <name>Q97UG6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO3054</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90487">
        <property type="entry name" value="C90487"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988426.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UG6"/>
    <dbReference type="FunCoup" id="Q97UG6">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3054"/>
    <dbReference type="PaxDb" id="273057-SSO3054"/>
    <dbReference type="EnsemblBacteria" id="AAK43154">
        <property type="protein sequence ID" value="AAK43154"/>
        <property type="gene ID" value="SSO3054"/>
    </dbReference>
    <dbReference type="GeneID" id="84059626"/>
    <dbReference type="KEGG" id="sso:SSO3054"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3152"/>
    <dbReference type="eggNOG" id="arCOG01617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_2"/>
    <dbReference type="InParanoid" id="Q97UG6"/>
    <dbReference type="PhylomeDB" id="Q97UG6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19079">
        <property type="entry name" value="AKR_EcYajO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000004">
        <property type="entry name" value="Oxidoreductase, aldo/keto reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF4">
        <property type="entry name" value="NAD(P)-LINKED OXIDOREDUCTASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="34"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="368" mass="42820" checksum="173575DF61129B36" modified="2001-10-01" version="1">MAKLLYLWYQTYKMCNKMEYMKFGNSGLKVSKFCLGTWHLPPSKERDSYGVYKVDEEESKKTIVKAIDLGVNFFDTANVYHGVMQGPDVFHVGNAERILGEAIKGYDRESLVLATKVRGKMAEHANGEGLSRKHIMWQVRESLKRLNTDYIDIYLMHRPDPDTPIEETMETFHDLVRQGKIHYIGESYFDPTDIPYLVKIAKDLHVPFIAMQEPYNFLERDIEKDKFILAKTYGLGIMAYIPLAQGVLTGKYLNTTPDMSRATYVPEISNRYFTKENFEVLREFHEIAKELGVTDTQLALAWIFKIQEINQITIVPIIGVTKINQLEENIESLNVKISNDTYKKLDEISKRVKVNWEIKYNKIIKRFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UJ2</accession>
    <name>Q97UJ2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1058</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO11734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90483">
        <property type="entry name" value="E90483"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UJ2"/>
    <dbReference type="STRING" id="273057.SSO11734"/>
    <dbReference type="PaxDb" id="273057-SSO11734"/>
    <dbReference type="EnsemblBacteria" id="AAK43124">
        <property type="protein sequence ID" value="AAK43124"/>
        <property type="gene ID" value="SSO11734"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11734"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3116"/>
    <dbReference type="eggNOG" id="arCOG02754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102439_1_0_2"/>
    <dbReference type="InParanoid" id="Q97UJ2"/>
    <dbReference type="PhylomeDB" id="Q97UJ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053172">
        <property type="entry name" value="Tn903_transposase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631:SF3">
        <property type="entry name" value="ISSOD12 TRANSPOSASE TNPA_ISSOD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="92" mass="11371" checksum="FF79B3A57C261F9E" modified="2001-10-01" version="1">MEKSKYKRDWSKYDENVITRYKLMFPFYVFEHWWDLLAEENRNARTKYKAPKEFNDFLAFLHIFLPYRAIEGVLRALEQLKIIPTSQQYGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UK5</accession>
    <name>Q97UK5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alpha-mannosidase</fullName>
            <ecNumber evidence="7">3.2.1.24</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO3006</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.24" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90481">
        <property type="entry name" value="E90481"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992657.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UK5"/>
    <dbReference type="FunCoup" id="Q97UK5">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3006"/>
    <dbReference type="CAZy" id="GH38">
        <property type="family name" value="Glycoside Hydrolase Family 38"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3006"/>
    <dbReference type="EnsemblBacteria" id="AAK43108">
        <property type="protein sequence ID" value="AAK43108"/>
        <property type="gene ID" value="SSO3006"/>
    </dbReference>
    <dbReference type="GeneID" id="1453027"/>
    <dbReference type="KEGG" id="sso:SSO3006"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3097"/>
    <dbReference type="eggNOG" id="arCOG03661">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003442_1_0_2"/>
    <dbReference type="InParanoid" id="Q97UK5"/>
    <dbReference type="PhylomeDB" id="Q97UK5"/>
    <dbReference type="BRENDA" id="3.2.1.24">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10789">
        <property type="entry name" value="GH38N_AMII_ER_cytosolic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000004">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000010">
        <property type="entry name" value="Cytosolic alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017:SF1">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="6">
        <location>
            <begin position="479"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="542"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="968" mass="111160" checksum="FF1C2C3C4F2CF1BA" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UM7</accession>
    <name>Q97UM7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Stage II sporulation protein M</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90478">
        <property type="entry name" value="B90478"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="AAK43081">
        <property type="protein sequence ID" value="AAK43081"/>
        <property type="gene ID" value="SSO2977"/>
    </dbReference>
    <dbReference type="GeneID" id="1453000"/>
    <dbReference type="KEGG" id="sso:SSO2977"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3068"/>
    <dbReference type="eggNOG" id="arCOG01995">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_865011_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UM7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002798">
        <property type="entry name" value="SpoIIM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01944">
        <property type="entry name" value="SpoIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="36611" checksum="B6BC3CBC7471C068" modified="2001-10-01" version="1">MRVLTKLILIVFVFEVVLFLIASAIPQNNPILVSQFNSTENQVLNQSYFGKVLMIFANNVRVGLLDFIPAVGMIILAISIYSTGAVLSAFSASLNVPGILSALGLMTLPHSWLELPSYAIAASSGLYIIIRPREWIRGLLTLIMVPIELFLAALVESGEFYVSNPYILWLYSIPAFVFLYFLYEFLQRRAENYIKVRAPVAPKQQNIVQLQTYADYLARYNQSWNTASYYETQGNLSEAMRYYWEAIFYLITAVGNKLGMPTLSKEDQDNVIRSVAYRVGNPQLYDIYNEAFKIRIENRINDFQIFKEYLSQLARYLNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UN3</accession>
    <name>Q97UN3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Quinol oxidase-2, cytochrome b (SoxG)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">soxG</name>
        <name evidence="3" type="ordered locus">SSO2970</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90477">
        <property type="entry name" value="D90477"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UN3"/>
    <dbReference type="FunCoup" id="Q97UN3">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2970"/>
    <dbReference type="PaxDb" id="273057-SSO2970"/>
    <dbReference type="EnsemblBacteria" id="AAK43075">
        <property type="protein sequence ID" value="AAK43075"/>
        <property type="gene ID" value="SSO2970"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2970"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3061"/>
    <dbReference type="eggNOG" id="arCOG01721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_530649_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UN3"/>
    <dbReference type="PhylomeDB" id="Q97UN3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.810.10:FF:000016">
        <property type="entry name" value="Cytochrome b/b6 domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.810.10">
        <property type="entry name" value="Cytochrome Bc1 Complex, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036150">
        <property type="entry name" value="Cyt_b/b6_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005797">
        <property type="entry name" value="Cyt_b/b6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027387">
        <property type="entry name" value="Cytb/b6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19271">
        <property type="entry name" value="CYTOCHROME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19271:SF16">
        <property type="entry name" value="CYTOCHROME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13631">
        <property type="entry name" value="Cytochrom_B_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81648">
        <property type="entry name" value="a domain/subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="Transmembrane di-heme cytochromes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51002">
        <property type="entry name" value="CYTB_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="437"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="482"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="524"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b/b6 N-terminal region profile" evidence="2">
        <location>
            <begin position="52"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="549" mass="61521" checksum="858F633674481044" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UT7</accession>
    <name>Q97UT7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Beta carotene hydroxylase (CrtZ)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">crtZ</name>
        <name evidence="6" type="ordered locus">SSO2906</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sterol desaturase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90469">
        <property type="entry name" value="H90469"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993320.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UT7"/>
    <dbReference type="STRING" id="273057.SSO2906"/>
    <dbReference type="PaxDb" id="273057-SSO2906"/>
    <dbReference type="EnsemblBacteria" id="AAK43015">
        <property type="protein sequence ID" value="AAK43015"/>
        <property type="gene ID" value="SSO2906"/>
    </dbReference>
    <dbReference type="GeneID" id="1452934"/>
    <dbReference type="KEGG" id="sso:SSO2906"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2996"/>
    <dbReference type="eggNOG" id="arCOG05415">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054855_1_1_2"/>
    <dbReference type="InParanoid" id="Q97UT7"/>
    <dbReference type="PhylomeDB" id="Q97UT7"/>
    <dbReference type="BRENDA" id="1.14.15.24">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045019">
        <property type="entry name" value="BETA-OHASE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31899">
        <property type="entry name" value="BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31899:SF9">
        <property type="entry name" value="BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="5">
        <location>
            <begin position="11"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="151" mass="17602" checksum="66B61EB88EDD5BC5" modified="2001-10-01" version="1">MMLIYYVGMAVLTFVGMEFVARLMHKYVMHGLLWFIHEDHHKEKQAELEKNDLFGLVFASVSVYLFFLGIQGSYVALSIAIGMSSYGIAYFFIHDMVIHDRHLHLRSWGLKHRPFKDLILVHDIHHKEGKGNWGFLFVIKGLDKVPILKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UU5</accession>
    <name>Q97UU5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Copper binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO11412</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90468">
        <property type="entry name" value="F90468"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990685.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UU5"/>
    <dbReference type="STRING" id="273057.SSO11412"/>
    <dbReference type="PaxDb" id="273057-SSO11412"/>
    <dbReference type="EnsemblBacteria" id="AAK43005">
        <property type="protein sequence ID" value="AAK43005"/>
        <property type="gene ID" value="SSO11412"/>
    </dbReference>
    <dbReference type="GeneID" id="1452924"/>
    <dbReference type="KEGG" id="sso:SSO11412"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2985"/>
    <dbReference type="eggNOG" id="arCOG02764">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134973_10_4_2"/>
    <dbReference type="InParanoid" id="Q97UU5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="2">
        <location>
            <begin position="5"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="82" mass="9123" checksum="9085303B66EAB39F" modified="2001-10-01" version="1">MAKIRKLEVEVRNMCCGHCATNVSKSVKEISGVREVDVDLREGLAFISIDEKADMNRVINNIREKITDLGYMVGEIKIIGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V08</accession>
    <name>Q97V08_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mechanosensitive ion channel MscS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2829</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90460">
        <property type="entry name" value="D90460"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990890.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V08"/>
    <dbReference type="STRING" id="273057.SSO2829"/>
    <dbReference type="PaxDb" id="273057-SSO2829"/>
    <dbReference type="EnsemblBacteria" id="AAK42939">
        <property type="protein sequence ID" value="AAK42939"/>
        <property type="gene ID" value="SSO2829"/>
    </dbReference>
    <dbReference type="GeneID" id="1452858"/>
    <dbReference type="KEGG" id="sso:SSO2829"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2914"/>
    <dbReference type="eggNOG" id="arCOG01573">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_893171_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V08"/>
    <dbReference type="PhylomeDB" id="Q97V08"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221:SF1">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="7">
        <location>
            <begin position="129"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="291" mass="31913" checksum="2D1F8A1C3D42EA20" modified="2001-10-01" version="1">MNYKPLIKVLLALVILALVDYLASVILKTLTFYFPQISTYTSDTFTAITVIIIAIGGFYIIRILQGLIYSQLLTRLEKSTASSIKVALDIIFYTILVLVILAALKVNLTGVLVGGAVGGIVIGLAVQTIAQNILSGILVTSSRTIRLNDAVSLISWIWGNPIIGEISKISLLFTEVKTINGNVIKIPNSAFLGNTVFQKLESEHSLVYPYQLLVNADVPANDLLNLAKAYINDFFSKEKIQLPEIYFTGKNGGTNAFTVILHFNDIKQLNGILNLINGAFDKAYWELKKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="146" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VC1</accession>
    <name>Q97VC1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter, ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2713</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99446">
        <property type="entry name" value="A99446"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988642.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VC1"/>
    <dbReference type="FunCoup" id="Q97VC1">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2713"/>
    <dbReference type="PaxDb" id="273057-SSO2713"/>
    <dbReference type="EnsemblBacteria" id="AAK42824">
        <property type="protein sequence ID" value="AAK42824"/>
        <property type="gene ID" value="SSO2713"/>
    </dbReference>
    <dbReference type="GeneID" id="1452743"/>
    <dbReference type="KEGG" id="sso:SSO2713"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2794"/>
    <dbReference type="eggNOG" id="arCOG00175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_2"/>
    <dbReference type="InParanoid" id="Q97VC1"/>
    <dbReference type="PhylomeDB" id="Q97VC1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055052">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015408">
        <property type="term" value="F:ABC-type ferric iron transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03259">
        <property type="entry name" value="ABC_Carb_Solutes_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000042">
        <property type="entry name" value="Maltose/maltodextrin ABC transporter, ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015853">
        <property type="entry name" value="ABC_transpr_FbpC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047641">
        <property type="entry name" value="ABC_transpr_MalK/UgpC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875">
        <property type="entry name" value="MALTODEXTRIN IMPORT ATP-BINDING PROTEIN MSMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875:SF15">
        <property type="entry name" value="TREHALOSE IMPORT ATP-BINDING PROTEIN SUGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="MOP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="2"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="314" mass="35425" checksum="7EC07C09F29CA748" modified="2001-10-01" version="1">MITLKEVTKFFGSFKVIDSISLNVEKGEFFVILGKSGSGKTTLLRLIAGLEKVSSGKILISNEDVTDLPPSKRDIGMVFQSYALYPNKKVFENLMIALENSEINKKDKEERIIEISKQLEIYHLMDKYPAQLSGGQQQRVAIAKALVKRPKVLLMDEPLSNLDVQLRYSARKLIKKVQKEFNITTIYVTHDSNEALAIADRIGVIDKGRLLQVGIPEDIYNNPLSKEVASLVGNPPMSFVKIDGKIIGVRPDNIIIKDNGTYEGIVSNCEFWGSYYLIYIDFIDNEVKAISKERIKEGSKVKFDFISYKVFSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VF6</accession>
    <name>Q97VF6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2671</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90441">
        <property type="entry name" value="E90441"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988588.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VF6"/>
    <dbReference type="FunCoup" id="Q97VF6">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2671"/>
    <dbReference type="TCDB" id="3.A.1.5.9">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2671"/>
    <dbReference type="EnsemblBacteria" id="AAK42788">
        <property type="protein sequence ID" value="AAK42788"/>
        <property type="gene ID" value="SSO2671"/>
    </dbReference>
    <dbReference type="GeneID" id="1452707"/>
    <dbReference type="KEGG" id="sso:SSO2671"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2752"/>
    <dbReference type="eggNOG" id="arCOG00748">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_8_0_2"/>
    <dbReference type="InParanoid" id="Q97VF6"/>
    <dbReference type="PhylomeDB" id="Q97VF6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42729">
        <property type="entry name" value="OLIGO/DIPEPTIDE TRANSPORT, PERMEASE PROTEIN (DPPC-2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42729:SF1">
        <property type="entry name" value="OLIGO_DIPEPTIDE TRANSPORT, PERMEASE PROTEIN (DPPC-2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="87"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="290" mass="31842" checksum="14C04C1869667289" modified="2001-10-01" version="1">MVQGKFFEYLRLLWDNKKSRVGLIITVFYILIAIFGQIIFPKTYSLPPSPTTIFMPPQLSNFYLIFGTGPFAESILVQIIQGAKSVIEISFLAGLFATLIGIVVGIIAGYLGGIIDNILMGITDIILTLPSLILIIIIVSAFKTSNPIFLSLILSITSWAGLARAVRSQVLVIRNSPAVEVLRVLGLSRKYIIFREVVPTLGSYIAIHYIFNVEAAVYAEVGLYYLGVLPYNPNNWGAMIQQALSYGAAAGGKAIYYLAFPTIVVAGFMSGLILLSYGIDEISNPRIRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VG9</accession>
    <name>Q97VG9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Quinol oxidase (SoxABC), cytochrome aa3 subunit (SoxB)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">soxB</name>
        <name evidence="9" type="ordered locus">SSO2657</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90439">
        <property type="entry name" value="H90439"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988569.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VG9"/>
    <dbReference type="FunCoup" id="Q97VG9">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2657"/>
    <dbReference type="PaxDb" id="273057-SSO2657"/>
    <dbReference type="EnsemblBacteria" id="AAK42775">
        <property type="protein sequence ID" value="AAK42775"/>
        <property type="gene ID" value="SSO2657"/>
    </dbReference>
    <dbReference type="GeneID" id="1452694"/>
    <dbReference type="GeneID" id="44128385"/>
    <dbReference type="KEGG" id="sso:SSO2657"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2739"/>
    <dbReference type="eggNOG" id="arCOG01238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_534881_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VG9"/>
    <dbReference type="PhylomeDB" id="Q97VG9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00919">
        <property type="entry name" value="Heme_Cu_Oxidase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.210.10:FF:000011">
        <property type="entry name" value="Cytochrome c oxidase subunit I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023615">
        <property type="entry name" value="Cyt_c_Oxase_su1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053628">
        <property type="entry name" value="Heme-copper_oxidase_sub1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041075">
        <property type="entry name" value="quin_ox_SoxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422:SF18">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="Cytochrome c oxidase subunit I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00077">
        <property type="entry name" value="COX1_CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="415"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="457"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit I profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="487"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="488"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="511" mass="56809" checksum="59E4E6D2A0B1E56C" modified="2001-10-01" version="1">MSQLLNQIKDTVIPKNTLNVVWLYTIGSIFWLGVLGIAAMNLRTYLTYNSNSPNVGELYYSALTIHGWAGMFGFVPLAAAAVIAFAMYKSNLSIVHTKLMAIFFWLSNIFLAIGLAGSPDMGWYMYPPLAIESGSNFHAFLFYTTPALMGMAYLSLTLAFILQTAMFIILIADAYATKPKNERLNIFGAYGVAFAIVIAITLPALAASTLWYTLYFFAGVPVNPLLWALLFWFYGHPVVYYVPFPLFGALYYYIPKYAGRSLFSEKWARWNIYLLSIGSMLIWVHHLQTWPLPIDLRIWVNLSTLILASGSGLTVLNLGLTILLSQKYNWKDPVGMGGLIALVGFILAGVQALVLPENSINPLFHNTYYVVGHFHLMIWTLIVMGFTTVFLDMLKSTFGGFNFSEQASKWMRIGMIWWTAPFLGVGYTMSVIGYLGMLRRMIAYPSVFQPYNLLESMLAEIGIPGLLVTIFVAIVDALAYASKQGIFSSPSPSTSPSNLPVMAKDGVKNNG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-11-16" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VN4</accession>
    <name>MDH_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Malate dehydrogenase</fullName>
            <ecNumber evidence="1">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">mdh</name>
        <name type="synonym">sqdB</name>
        <name type="ordered locus">SSO2585</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible oxidation of malate to oxaloacetate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LDH/MDH superfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK42709" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42709.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90431">
        <property type="entry name" value="F90431"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VN4"/>
    <dbReference type="SMR" id="Q97VN4"/>
    <dbReference type="FunCoup" id="Q97VN4">
        <property type="interactions" value="182"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2585"/>
    <dbReference type="PaxDb" id="273057-SSO2585"/>
    <dbReference type="EnsemblBacteria" id="AAK42709">
        <property type="protein sequence ID" value="AAK42709"/>
        <property type="gene ID" value="SSO2585"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2585"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2666"/>
    <dbReference type="eggNOG" id="arCOG00246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045401_2_1_2"/>
    <dbReference type="InParanoid" id="Q97VN4"/>
    <dbReference type="PhylomeDB" id="Q97VN4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042867">
        <property type="term" value="P:pyruvate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00300">
        <property type="entry name" value="LDH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128">
        <property type="entry name" value="L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128:SF16">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="chain" id="PRO_0000113493" description="Malate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="118"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O08349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P61889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="308" mass="33967" checksum="287C1781B4178397" modified="2001-11-16" version="2">MVKIAFIGVGKIGQTIAYSVIFDGLASEAILYDIVPELPEKFEHELRHAIATRGLSTEVIGTNSLDDVTNADIILIMAGKPRKPGMSRRDLFIDNAKIQIDLAKQLPPKNPGALYIMVANPVDMMASVFMRFSKQFTISTGDAVETMRLRSYIAKKLKVPANKVTGYVAGEHGEDAVVLWSTVKVNGKPFEEVAKGLTKEEVENYVKSIPGEIIRVMGGTTWGPATIIKDIVRSVVFNEGRVMSIATPRTYQGEIIHISVPIVVGAEIGPSLEGVLPESDRQRLNKSVEDFYRVYKENLDHLLQTVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VR2</accession>
    <name>Q97VR2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Serine protease, subtilase family, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO2551</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90428">
        <property type="entry name" value="A90428"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990123.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2551"/>
    <dbReference type="PaxDb" id="273057-SSO2551"/>
    <dbReference type="EnsemblBacteria" id="AAK42680">
        <property type="protein sequence ID" value="AAK42680"/>
        <property type="gene ID" value="SSO2551"/>
    </dbReference>
    <dbReference type="GeneID" id="1454007"/>
    <dbReference type="KEGG" id="sso:SSO2551"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2632"/>
    <dbReference type="eggNOG" id="arCOG03665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006754_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VR2"/>
    <dbReference type="PhylomeDB" id="Q97VR2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000027">
        <property type="entry name" value="Peptidase S53 propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017001">
        <property type="entry name" value="Pept_S53_physarolisin-II_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF15">
        <property type="entry name" value="TRIPEPTIDYL-PEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032623">
        <property type="entry name" value="Peptidase_SSO2181_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1282"/>
            <end position="1304"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S53" evidence="9">
        <location>
            <begin position="208"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="1308" mass="141890" checksum="B248C3BB1ED28E3C" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VS6</accession>
    <name>Q97VS6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enoyl CoA hydratase (PaaF-5)</fullName>
            <ecNumber evidence="2">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">paaF-5</name>
        <name evidence="2" type="ordered locus">SSO2535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99426">
        <property type="entry name" value="A99426"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990982.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VS6"/>
    <dbReference type="STRING" id="273057.SSO2535"/>
    <dbReference type="PaxDb" id="273057-SSO2535"/>
    <dbReference type="EnsemblBacteria" id="AAK42664">
        <property type="protein sequence ID" value="AAK42664"/>
        <property type="gene ID" value="SSO2535"/>
    </dbReference>
    <dbReference type="GeneID" id="1453991"/>
    <dbReference type="KEGG" id="sso:SSO2535"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2611"/>
    <dbReference type="eggNOG" id="arCOG00242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_3_2"/>
    <dbReference type="InParanoid" id="Q97VS6"/>
    <dbReference type="PhylomeDB" id="Q97VS6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43802">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43802:SF1">
        <property type="entry name" value="IP11341P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="246" mass="27182" checksum="BC96CE8F7E81DD41" modified="2001-10-01" version="1">MTKILLEKRKDVCWITLNRPEKLNALDKESWSLLANHLGECNNDQSISAIVLTGNGRAFSAGDDINAMLELKDQKDALDFFNTLYSAVESLVDLKKPLVCAVNGLAYGGGCEILLFCDIVIAVKDATFSIPEGRLGLIPPIAISLGYLILGRSIARLALTGDSITAEEAKMIGLVDIVVANEDLLTEVERQLEKIKSIDRSSILTIKNWLRDDKEKIRKAVMELALMALGNSAKEKMKEFMNRKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="137" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VW0</accession>
    <name>Q97VW0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alcohol dehydrogenase (Zn containing) (Adh-8)</fullName>
            <ecNumber evidence="8">1.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">adh-8</name>
        <name evidence="8" type="ordered locus">SSO2494</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90421">
        <property type="entry name" value="G90421"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923898.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VW0"/>
    <dbReference type="FunCoup" id="Q97VW0">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2494"/>
    <dbReference type="PaxDb" id="273057-SSO2494"/>
    <dbReference type="EnsemblBacteria" id="AAK42630">
        <property type="protein sequence ID" value="AAK42630"/>
        <property type="gene ID" value="SSO2494"/>
    </dbReference>
    <dbReference type="GeneID" id="7808249"/>
    <dbReference type="KEGG" id="sso:SSO2494"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2575"/>
    <dbReference type="eggNOG" id="arCOG01455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_0_2"/>
    <dbReference type="InParanoid" id="Q97VW0"/>
    <dbReference type="PhylomeDB" id="Q97VW0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08259">
        <property type="entry name" value="Zn_ADH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053496">
        <property type="entry name" value="Acryloyl-CoA_Reductase_Zn-ADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041172">
        <property type="entry name" value="AcrlCoa_red_Thmprot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010344">
        <property type="entry name" value="PRK13771.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="8"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="336" mass="36468" checksum="DB00AE2369C49F86" modified="2001-10-01" version="1">MKAVILPAHKQGYRIEEVPDPKPGKDEAIIKVNKAALCYRDLLQIKGFYPRSKYPLILGHEVVGTVEEVGEDVKDFKKGDRVTSMLFVPDWSCEYCKSGEEVYCKNRVVYAQELDGFFAEKAKVKASSLVKVPESVSDEGAVIVPCVAAMVYRGLKKAGIKKGEIVLVTGASGGVGIHAIQVAKALGAKVIGVTSGESKAKIVSKYADYVIVGEKFSEEAKKIGDVSIVIENVGPYTLEESMKSLRSGGKIIQIGNLDPSVTFNLRLGYIILKDISLMGHIGANKNDIIKTLNLVKEGKIKPVIGSEVSLENFGKALDLLNDSKNRYGKILISNSF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-30" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W44</accession>
    <name>PANB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-methyl-2-oxobutanoate hydroxymethyltransferase</fullName>
            <ecNumber evidence="1">2.1.2.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ketopantoate hydroxymethyltransferase</fullName>
            <shortName evidence="1">KPHMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">panB</name>
        <name type="ordered locus">SSO2400</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = 2-dehydropantoate + (6S)-5,6,7,8-tetrahydrofolate</text>
            <dbReference type="Rhea" id="RHEA:11824"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodecamer; pentamer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PanB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.11" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90411">
        <property type="entry name" value="C90411"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989439.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W44"/>
    <dbReference type="SMR" id="Q97W44"/>
    <dbReference type="FunCoup" id="Q97W44">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2400"/>
    <dbReference type="PaxDb" id="273057-SSO2400"/>
    <dbReference type="EnsemblBacteria" id="AAK42546">
        <property type="protein sequence ID" value="AAK42546"/>
        <property type="gene ID" value="SSO2400"/>
    </dbReference>
    <dbReference type="GeneID" id="1453873"/>
    <dbReference type="GeneID" id="44128121"/>
    <dbReference type="KEGG" id="sso:SSO2400"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2481"/>
    <dbReference type="eggNOG" id="arCOG00584">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036645_1_0_2"/>
    <dbReference type="InParanoid" id="Q97W44"/>
    <dbReference type="PhylomeDB" id="Q97W44"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003864">
        <property type="term" value="F:3-methyl-2-oxobutanoate hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06557">
        <property type="entry name" value="KPHMT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000052">
        <property type="entry name" value="3-methyl-2-oxobutanoate hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00156">
        <property type="entry name" value="PanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003700">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00222">
        <property type="entry name" value="panB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001452">
        <property type="entry name" value="PRK00311.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881:SF0">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02548">
        <property type="entry name" value="Pantoate_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000388">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000184923" description="3-methyl-2-oxobutanoate hydroxymethyltransferase">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="45"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00156"/>
        </source>
    </evidence>
    <sequence length="267" mass="29624" checksum="D7CD0CFEB331DCBD" modified="2001-10-01" version="1">MKKVTIRDFIKKKSMKEKITMLTAYDYPTAKIISNTTLDSILVGDSLGMVVLGYTNTLNVTMRDMISHTRAVARANPPQLIVSDMPFLSYEIDVKSAVKNAGLLVKAGSDAVKLEGGEEIKDIIRAIVKAGIPVMGHIGLTPQRFLRLGGFRTIGKTKQEEEQLIKDSLELEDAGVFSIVIENTYVDIAKRITEKLNVPTICIGAGPYCDGQVLVIHDLLGLSEVTPYFAKSYVNLKEIISSAINQYIIDVKTNKFPEKQHYKERES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W99</accession>
    <name>Q97W99_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CobQ/CobB/MinD/ParA nucleotide binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2336</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90404">
        <property type="entry name" value="B90404"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989521.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W99"/>
    <dbReference type="SMR" id="Q97W99"/>
    <dbReference type="STRING" id="273057.SSO2336"/>
    <dbReference type="PaxDb" id="273057-SSO2336"/>
    <dbReference type="EnsemblBacteria" id="AAK42489">
        <property type="protein sequence ID" value="AAK42489"/>
        <property type="gene ID" value="SSO2336"/>
    </dbReference>
    <dbReference type="GeneID" id="1453816"/>
    <dbReference type="KEGG" id="sso:SSO2336"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2423"/>
    <dbReference type="eggNOG" id="arCOG07293">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1346462_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W99"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02042">
        <property type="entry name" value="ParAB_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF10">
        <property type="entry name" value="ATPASE INVOLVED IN CHROMOSOME PARTITIONING, PARA_MIND FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="1">
        <location>
            <begin position="9"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="206" mass="23490" checksum="A2A9F6E7F684C48B" modified="2001-10-01" version="1">MITNIIRLLSIKGGVGKSSIAYALARIISASGYKVLFLDMDNLHTISRVLGVKDCELTYTRDFFVFACDDFSKISFNSYEYVIIDTYSGISSETLSAISGDHVYNIFISDFSSIDNTLNYIKEWNGKKTTNFLVVNMVIREDNENDILLRRFFDSLLLKINVAINKIFLFFFDENYYGSYKVDLNHIELLASYVLGEMSESDLNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WB7</accession>
    <name>Q97WB7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flagella-related protein I, putative (FlaI)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">FlaI</name>
        <name evidence="3" type="ordered locus">SSO2316</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90401">
        <property type="entry name" value="H90401"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923830.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WB7"/>
    <dbReference type="STRING" id="273057.SSO2316"/>
    <dbReference type="PaxDb" id="273057-SSO2316"/>
    <dbReference type="EnsemblBacteria" id="AAK42471">
        <property type="protein sequence ID" value="AAK42471"/>
        <property type="gene ID" value="SSO2316"/>
    </dbReference>
    <dbReference type="GeneID" id="1453798"/>
    <dbReference type="KEGG" id="sso:SSO2316"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2405"/>
    <dbReference type="eggNOG" id="arCOG01817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_2_2_2"/>
    <dbReference type="InParanoid" id="Q97WB7"/>
    <dbReference type="PhylomeDB" id="Q97WB7"/>
    <dbReference type="BRENDA" id="3.6.4.B6">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01130">
        <property type="entry name" value="VirB11-like_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.370:FF:000001">
        <property type="entry name" value="Secretion system protein E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002252">
        <property type="entry name" value="Type IV secretion system protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF14">
        <property type="entry name" value="FLAGELLA ACCESSORY PROTEIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial type II secretion system protein E" evidence="2">
        <location>
            <begin position="187"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="513" mass="58665" checksum="9C2DAADF2106D352" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WD5</accession>
    <name>Q97WD5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein kinase, putative</fullName>
            <ecNumber evidence="3">2.7.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2291</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.1.-" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90399">
        <property type="entry name" value="F90399"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991821.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WD5"/>
    <dbReference type="STRING" id="273057.SSO2291"/>
    <dbReference type="PaxDb" id="273057-SSO2291"/>
    <dbReference type="EnsemblBacteria" id="AAK42453">
        <property type="protein sequence ID" value="AAK42453"/>
        <property type="gene ID" value="SSO2291"/>
    </dbReference>
    <dbReference type="GeneID" id="1453780"/>
    <dbReference type="KEGG" id="sso:SSO2291"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2385"/>
    <dbReference type="eggNOG" id="arCOG03682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_491463_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WD5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF18">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="244"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="554" mass="62887" checksum="5AA2EDCA3547FA78" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WE8</accession>
    <name>Q97WE8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99398">
        <property type="entry name" value="A99398"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WE8"/>
    <dbReference type="PaxDb" id="273057-SSO2279"/>
    <dbReference type="EnsemblBacteria" id="AAK42440">
        <property type="protein sequence ID" value="AAK42440"/>
        <property type="gene ID" value="SSO2279"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2279"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2371"/>
    <dbReference type="eggNOG" id="arCOG05899">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_663271_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WE8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="433" mass="48091" checksum="20696E1CAC52B855" modified="2001-10-01" version="1">MIYGVLCMRSVTISILALIITWGILGSLIITQANTTIVNTTTIPTSITYTVKLINSTTVANKTITHVYNYTLYYQVTKFVQNTVYVNISGNFTNGTLKLGNTVYIKKGVYEVNLLYQPLSIYYPFIEPSIIFNTSYGIISPNETIALVYKGKTFNMINGVNYTSYNYSGNVNGQQISVNILQNGIISKLENGSLELILESFSNLSTIPLTTVTNSQYMNFLNRSYLYAVYNYSPLSNSLIPEGYLQISYPVVIGNNTITAQITQIQLRAGKQLALPASLLGEPVNFVLYINQPQNIPTSFIVNVGAKNITWNSNVFTLVGESKVSTPIGTFIAYQYVHNISQNQAQQVLYFTPNGTLIKEEVVEIINSTTLPSFSLSFAGNTYISPFETYPNVFNYTNTSLPYKVIAPNLSFTISIVITIIIVAILVVLHKRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WJ8</accession>
    <name>NADK_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD kinase</fullName>
            <ecNumber evidence="1">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nadK</name>
        <name type="ordered locus">SSO2219</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + ATP = ADP + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90391">
        <property type="entry name" value="E90391"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992063.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WJ8"/>
    <dbReference type="SMR" id="Q97WJ8"/>
    <dbReference type="FunCoup" id="Q97WJ8">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2219"/>
    <dbReference type="PaxDb" id="273057-SSO2219"/>
    <dbReference type="EnsemblBacteria" id="AAK42388">
        <property type="protein sequence ID" value="AAK42388"/>
        <property type="gene ID" value="SSO2219"/>
    </dbReference>
    <dbReference type="GeneID" id="1453715"/>
    <dbReference type="KEGG" id="sso:SSO2219"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2314"/>
    <dbReference type="eggNOG" id="arCOG01348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008831_0_3_2"/>
    <dbReference type="InParanoid" id="Q97WJ8"/>
    <dbReference type="PhylomeDB" id="Q97WJ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF43">
        <property type="entry name" value="BIFUNCTIONAL NADP PHOSPHATASE_NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000120709" description="NAD kinase">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="45"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="110"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="149"/>
            <end position="154"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <sequence length="249" mass="27890" checksum="D4AD6A9F7DCD8A49" modified="2001-10-01" version="1">MRVKIVSKPTSQLNYTVEKIKNISNKLGFEVVDIDFDYVIAVGGDGTLLRAVKLGKPVIAIKAGRRGLLMDVPVDKIEDALLRLKKGDYNEEEYMLLEMVHNDKVELGFNEIGILYDRPEAIKVGISFDTERVSVEGDGVLVSTPQGSSGWGMSATNSLLYKDLNAIEIIFVNPIFYYLRSVVIPPKSLILRLEDKGYPQTARVVVDGEVVTLIKTNQEITVRVSQHKAKILRFFKLDLIGEVLHAYHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WK1</accession>
    <name>Q97WK1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NifS protein homolog (NifS)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nifS</name>
        <name evidence="3" type="ordered locus">SSO2215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90391">
        <property type="entry name" value="B90391"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992067.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WK1"/>
    <dbReference type="FunCoup" id="Q97WK1">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2215"/>
    <dbReference type="PaxDb" id="273057-SSO2215"/>
    <dbReference type="DNASU" id="1453712"/>
    <dbReference type="EnsemblBacteria" id="AAK42385">
        <property type="protein sequence ID" value="AAK42385"/>
        <property type="gene ID" value="SSO2215"/>
    </dbReference>
    <dbReference type="GeneID" id="1453712"/>
    <dbReference type="KEGG" id="sso:SSO2215"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2311"/>
    <dbReference type="eggNOG" id="arCOG00065">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_2_1_2"/>
    <dbReference type="InParanoid" id="Q97WK1"/>
    <dbReference type="PhylomeDB" id="Q97WK1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006534">
        <property type="term" value="P:cysteine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF8">
        <property type="entry name" value="CYSTEINE DESULFURASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="2">
        <location>
            <begin position="21"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="376" mass="42569" checksum="414EF139CF37F21E" modified="2001-10-01" version="1">MRLRDPREFRENVPVTRKYVYLNHASVSPTPLPSLFEAYRYLYEVANRGSIAVNEEEEDELYHIRSKISNLVGAYSDEISLIPNTSYGVNLVAHGLEWKGDDNIVTDNLEFPTVVYPFLKLTKKGVKINIVETNPYTFEEDIISHIDKNTRLVAISHVSFNTGLKVDVRKIVKAARENNTLVLLDIIQSAGAVKINVKELGIDFAIAGGYKWLMSPQGSGFIYVKRGLIEDPPFYGWKTSADYLDFNPNKFTLEKGPRRFEIGTVDLAANLSLAKSCEIIGENMELIESSVTNLSQFAIRLAKDHSMEVITPEDKRAGIVIVKVKKPKEIAKELLKENIVVSPRGEGIRISTHFYNTEEEVQKTIEKISELERKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WQ9</accession>
    <name>Q97WQ9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acetyl-CoA synthetase (Acetate-CoA ligase) (AcsA-6)</fullName>
            <ecNumber evidence="8">6.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">acsA-6</name>
        <name evidence="8" type="ordered locus">SSO2059</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="PIR" id="D90373">
        <property type="entry name" value="D90373"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923763.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WQ9"/>
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        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2059"/>
    <dbReference type="PaxDb" id="273057-SSO2059"/>
    <dbReference type="EnsemblBacteria" id="AAK42243">
        <property type="protein sequence ID" value="AAK42243"/>
        <property type="gene ID" value="SSO2059"/>
    </dbReference>
    <dbReference type="GeneID" id="1453570"/>
    <dbReference type="KEGG" id="sso:SSO2059"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2138"/>
    <dbReference type="eggNOG" id="arCOG01529">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_000022_3_6_2"/>
    <dbReference type="InParanoid" id="Q97WQ9"/>
    <dbReference type="PhylomeDB" id="Q97WQ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ACAS_N"/>
    </dbReference>
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        <property type="entry name" value="AMP-bd_C"/>
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        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
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        <property type="entry name" value="AMP-binding_CS"/>
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    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
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        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
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        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF232">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
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    </dbReference>
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        <property type="entry name" value="AMP-binding_C"/>
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    </dbReference>
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        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="7">
        <location>
            <begin position="12"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="69"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="514"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="622" mass="70115" checksum="45B6E4BA06452ECD" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WR1</accession>
    <name>Q97WR1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sugar transport related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2057</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="AAK42241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="273057.SSO2057"/>
    <dbReference type="PaxDb" id="273057-SSO2057"/>
    <dbReference type="EnsemblBacteria" id="AAK42241">
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        <property type="gene ID" value="SSO2057"/>
    </dbReference>
    <dbReference type="GeneID" id="84061139"/>
    <dbReference type="KEGG" id="sso:SSO2057"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2136"/>
    <dbReference type="eggNOG" id="arCOG02687">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_2"/>
    <dbReference type="InParanoid" id="Q97WR1"/>
    <dbReference type="PhylomeDB" id="Q97WR1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000912">
        <property type="entry name" value="Major facilitator superfamily MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF34">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="455"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="28"/>
            <end position="483"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="492" mass="52667" checksum="C33B67B234F9AC29" modified="2001-10-01" version="1">MAEKAAVKAGEAIARMDRIPIWGLSYIFIGILGIGFLFTFYDIFDINVSFIQTALTLFHVSNPTSPQIPALLGPIVLLNLVGYIIGALILSPISDVIGRRRMLMITMIITGLGSLYNALANNYVNFLVARTITGIGVGADLAIVNTYIGEVAPINGRARYTSLVFLFSTLGATLGIWLGLFLTTPSAPFPLGLPFALGGSGFFAVNGWRVMYGIGALLALIGLLLRFSLPESPRWLISKGKLTEAERIVEVMEKKATGKLGNLPPLPNIIQPYIAERYSYLDALKTIFSSSLYIKRFGILVSMWLFGYMTVYTLAAGLTSVLASLGFAPPEAGMIAAIGVIGFILVPITTFFVGDRLERKTWTVISVVFTIIGGLLIAIAGTNIALSFLGSIILFYGFNLWVPTSYAWTAESFPTRARSTGFALCDGVGHIGGGIGIIAISSFIGSLIASGVTTALAIEVFLLIALFQVISTIIAIAIGHRSANKRLDEVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WW1</accession>
    <name>Q97WW1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ATP-dependent RNA helicase homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1999</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90366">
        <property type="entry name" value="F90366"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WW1"/>
    <dbReference type="STRING" id="273057.SSO1999"/>
    <dbReference type="PaxDb" id="273057-SSO1999"/>
    <dbReference type="EnsemblBacteria" id="AAK42189">
        <property type="protein sequence ID" value="AAK42189"/>
        <property type="gene ID" value="SSO1999"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1999"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2076"/>
    <dbReference type="eggNOG" id="arCOG01444">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031100_1_0_2"/>
    <dbReference type="InParanoid" id="Q97WW1"/>
    <dbReference type="PhylomeDB" id="Q97WW1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052511">
        <property type="entry name" value="ATP-dep_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054712">
        <property type="entry name" value="Cas3-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006474">
        <property type="entry name" value="Helicase_Cas3_CRISPR-ass_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01587">
        <property type="entry name" value="cas3_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962:SF5">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22590">
        <property type="entry name" value="Cas3-like_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="41"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="245"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="515" mass="57995" checksum="40ACB477AED99EB9" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WY9</accession>
    <name>Q97WY9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1970</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90363">
        <property type="entry name" value="C90363"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991922.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WY9"/>
    <dbReference type="STRING" id="273057.SSO1970"/>
    <dbReference type="PaxDb" id="273057-SSO1970"/>
    <dbReference type="EnsemblBacteria" id="AAK42162">
        <property type="protein sequence ID" value="AAK42162"/>
        <property type="gene ID" value="SSO1970"/>
    </dbReference>
    <dbReference type="GeneID" id="1453489"/>
    <dbReference type="KEGG" id="sso:SSO1970"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2047"/>
    <dbReference type="eggNOG" id="arCOG00729">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121774_5_0_2"/>
    <dbReference type="InParanoid" id="Q97WY9"/>
    <dbReference type="PhylomeDB" id="Q97WY9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09873">
        <property type="entry name" value="PIN_Pae0151-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044153">
        <property type="entry name" value="PIN_Pae0151-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051619">
        <property type="entry name" value="TypeII_TA_RNase_PINc/VapC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901:SF1">
        <property type="entry name" value="EXONUCLEASE VAPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901">
        <property type="entry name" value="RIBONUCLEASE VAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="2">
        <location>
            <begin position="20"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="130" mass="15106" checksum="5B0B966B366BD159" modified="2001-10-01" version="1">MKDKEFLFDASALYSLLDYVDKIDLKKIHILTLTFYEVGNVIWKEYYIHKKVKDPITLSMLFHKLMRKLNIVEDPPLEGVMRIAVERGLTYYDASYAYVAESLGLILVSNDKELIRKANAISLKDLIKSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X46</accession>
    <name>Q97X46_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4898 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90355">
        <property type="entry name" value="G90355"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923645.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X46"/>
    <dbReference type="PaxDb" id="273057-SSO1910"/>
    <dbReference type="EnsemblBacteria" id="AAK42102">
        <property type="protein sequence ID" value="AAK42102"/>
        <property type="gene ID" value="SSO1910"/>
    </dbReference>
    <dbReference type="GeneID" id="1453429"/>
    <dbReference type="KEGG" id="sso:SSO1910"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1971"/>
    <dbReference type="eggNOG" id="arCOG08428">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2091409_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X46"/>
    <dbReference type="PhylomeDB" id="Q97X46"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032603">
        <property type="entry name" value="DUF4898"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16239">
        <property type="entry name" value="DUF4898"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="13182" checksum="B10F1A86D8A61090" modified="2001-10-01" version="1">MMKKLSVKELGDYLDDDLLKLLNKEDIRHIYLINVKLISNFEKFFTTFLPDSIKYFVVISSDLPIKLIKESLARAKDALEVSCYISSKLPPKSMIVIGLKSVSKKEEVKEPSSSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X51</accession>
    <name>Q97X51_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetyl-CoA synthetase (Acetate-CoA ligase) carboxy-end. (AcsA-3)</fullName>
            <ecNumber evidence="6">6.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">acsA-3</name>
        <name evidence="6" type="ordered locus">SSO9201</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99355">
        <property type="entry name" value="A99355"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X51"/>
    <dbReference type="STRING" id="273057.SSO9201"/>
    <dbReference type="PaxDb" id="273057-SSO9201"/>
    <dbReference type="EnsemblBacteria" id="AAK42096">
        <property type="protein sequence ID" value="AAK42096"/>
        <property type="gene ID" value="SSO9201"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO9201"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1965"/>
    <dbReference type="eggNOG" id="arCOG04201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2679114_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X51"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051087">
        <property type="entry name" value="Mitochondrial_ACSM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605:SF10">
        <property type="entry name" value="ACYL-COA SYNTHETASE MEDIUM CHAIN FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605">
        <property type="entry name" value="ACYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="5">
        <location>
            <begin position="10"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="74" mass="8636" checksum="15FFE3E32D631F21" modified="2001-10-01" version="1">MSKIGETPLIRGHVLHAYIVLKSGYTPSEELKKEIINFVNSKYSRHVHLEKVDFVDKLPKTESGKIQRYLLRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X63</accession>
    <name>Q97X63_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90353">
        <property type="entry name" value="B90353"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X63"/>
    <dbReference type="STRING" id="273057.SSO1890"/>
    <dbReference type="PaxDb" id="273057-SSO1890"/>
    <dbReference type="EnsemblBacteria" id="AAK42081">
        <property type="protein sequence ID" value="AAK42081"/>
        <property type="gene ID" value="SSO1890"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1890"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1946"/>
    <dbReference type="eggNOG" id="arCOG03660">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726883_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X63"/>
    <dbReference type="PhylomeDB" id="Q97X63"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052714">
        <property type="entry name" value="MFS_Exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531">
        <property type="entry name" value="QUINOLENE RESISTANCE PROTEIN NORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531:SF1">
        <property type="entry name" value="QUINOLENE RESISTANCE PROTEIN NORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="372"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="406" mass="45280" checksum="63E03EAF68B5D40C" modified="2001-10-01" version="1">MFTTFVSKFKFKLSTTYISTMDSSKHSLIYSAIILVLMTISARATNNMVTTTVPLLAKYSLQLSNSMVGLLSALLFTFNFIATTFINPELNSKLRKRAFVISNIIILLSLPLYYLSNVISIWIISIFAGIAFGLVMPNLITAASLANDKKSVERLLSLYSTSLSTSLILGPAIEVYLLTRYDYRDVFLWFIPIAVIGVIIASRIRFPDVKRESSGMSVIKNKGLMAASLSITTYNVPFAAFTTFLAILAKDRFNLPSFDAYFVFLPFYIMSFLTRLTMTIRPFENLRMPMLISIIITILGLFGLLYAPNYSIFLAVIALLGIPHGSIFPMATIMISRATSIAERNAVNSYFSAYNNLLFLIVPAIIGFVSEIIGLTFSISALVIPVAITAVVFFKMFWNDNIMVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X74</accession>
    <name>Q97X74_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1879</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90351">
        <property type="entry name" value="E90351"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991421.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X74"/>
    <dbReference type="STRING" id="273057.SSO1879"/>
    <dbReference type="PaxDb" id="273057-SSO1879"/>
    <dbReference type="EnsemblBacteria" id="AAK42068">
        <property type="protein sequence ID" value="AAK42068"/>
        <property type="gene ID" value="SSO1879"/>
    </dbReference>
    <dbReference type="GeneID" id="1453395"/>
    <dbReference type="KEGG" id="sso:SSO1879"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1931"/>
    <dbReference type="eggNOG" id="arCOG00877">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025659_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X74"/>
    <dbReference type="PhylomeDB" id="Q97X74"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050547">
        <property type="entry name" value="DEAD_box_RNA_helicases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47963:SF8">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47963">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 47, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="30"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="281"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="665" mass="76436" checksum="0746CDFE789120E8" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XF3</accession>
    <name>Q97XF3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRISPR system endoribonuclease Csx1 CARF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90340">
        <property type="entry name" value="F90340"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923588.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XF3"/>
    <dbReference type="STRING" id="273057.SSO1787"/>
    <dbReference type="PaxDb" id="273057-SSO1787"/>
    <dbReference type="EnsemblBacteria" id="AAK41981">
        <property type="protein sequence ID" value="AAK41981"/>
        <property type="gene ID" value="SSO1787"/>
    </dbReference>
    <dbReference type="GeneID" id="1454760"/>
    <dbReference type="KEGG" id="sso:SSO1787"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1842"/>
    <dbReference type="eggNOG" id="arCOG03433">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1149855_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XF3"/>
    <dbReference type="PhylomeDB" id="Q97XF3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10640">
        <property type="entry name" value="SSO1389-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013383">
        <property type="entry name" value="CRISPR-assoc_prot_DxTHG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052875">
        <property type="entry name" value="CRISPR_assoc_ribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053857">
        <property type="entry name" value="Csx1_CARF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02549">
        <property type="entry name" value="CRISPR_DxTHG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37169:SF1">
        <property type="entry name" value="CRISPR SYSTEM ENDORIBONUCLEASE CSX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37169">
        <property type="entry name" value="CRISPR SYSTEM ENDORIBONUCLEASE CSX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22230">
        <property type="entry name" value="Csx1_CARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160980">
        <property type="entry name" value="SSO1389-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR system endoribonuclease Csx1 CARF" evidence="1">
        <location>
            <begin position="3"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="241" mass="27885" checksum="01B7922CDED52F38" modified="2001-10-01" version="1">MVVYVSTWGDPSGWFEVEYKRPDKEIKSFSTISTYDNASKIILIVQDSVLTPQSKPKNKVAENCSKLKTPSDYESWVNKVKEYISCIVENALNKEAANKTRIIVIPAVGKINDFNYGKIELKERELPSYLYAYIVETLLVQKLYEELKDADDDEIVLDTTHGVNYLPIIVFRVLYNLTSLLDLKFKVINYVPTNLYKEYTYMEIFKREEKKNTFDLTQINVGLSDDPIKRIIIKSLKLNAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XI3</accession>
    <name>Q97XI3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Potassium channel protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1757</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90336">
        <property type="entry name" value="G90336"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923573.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XI3"/>
    <dbReference type="STRING" id="273057.SSO1757"/>
    <dbReference type="PaxDb" id="273057-SSO1757"/>
    <dbReference type="EnsemblBacteria" id="AAK41950">
        <property type="protein sequence ID" value="AAK41950"/>
        <property type="gene ID" value="SSO1757"/>
    </dbReference>
    <dbReference type="GeneID" id="1454729"/>
    <dbReference type="KEGG" id="sso:SSO1757"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1798"/>
    <dbReference type="eggNOG" id="arCOG01958">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_822869_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XI3"/>
    <dbReference type="PhylomeDB" id="Q97XI3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050721">
        <property type="entry name" value="Trk_Ktr_HKT_K-transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833:SF9">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN YUGO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="TrkA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0407">Ion channel</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="2">
        <location>
            <begin position="127"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="3">
        <location>
            <begin position="283"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="336" mass="37250" checksum="3CFE982822E21748" modified="2001-10-01" version="1">MNKERVWNRIIIPLLENLVAPYSVVRRIYVQIILLGVAVLINAVVFITYQHLDWISAIYAGVNVVTTVGLYSPNIYQMPSNEKIILTLTIILAVGLYTSIVQSIIATLISRSTWNDVKARWRGKHMKGHTILIGNGSELLSAVRRLNRLSIDYIVLTTSKDIADKIKGDNVILGDPKDDNTLLSAGVKEAKNAIVMLEDDMEALLVTLKLQKLNPPLQVIVAIRDDSLRDLFNTAGADLVISREDIIGRIAASAAVSTNIAGLIFPERSGDMIIGIFHVTKKRRIKDLPDGVVPLAIIRGNRIDPFFTKETELNIGEQLVVLGNPSTFREVKELLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-10-02" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XI8</accession>
    <name>Q97XI8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1395</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1748</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90336">
        <property type="entry name" value="A90336"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XI8"/>
    <dbReference type="PaxDb" id="273057-SSO1748"/>
    <dbReference type="EnsemblBacteria" id="AAK41944">
        <property type="protein sequence ID" value="AAK41944"/>
        <property type="gene ID" value="SSO1748"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1748"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1792"/>
    <dbReference type="eggNOG" id="arCOG02127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431707_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XI8"/>
    <dbReference type="PhylomeDB" id="Q97XI8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13551">
        <property type="entry name" value="HTH_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="189" mass="22509" checksum="6F944B28440CDD52" modified="2001-10-01" version="1">MDVLRTLKRRIRKLIEKKPKNLNETKARGILLYLKGMNTRKIAKILQVHLSTVYRWINQFEKEGEKCLCYKKRKGRNKKVDEKEIKVEELQGKNIWEAKAYIEEKFHVKLSYVTAWRIARKRLKIPYIKPYNKKRPFNADNILRERLKGVIILRQTLKGKKTSLSIDVQRFTAPLHTIRKVIYSLGYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XQ0</accession>
    <name>Q97XQ0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Second ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1659</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90327">
        <property type="entry name" value="B90327"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923554.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XQ0"/>
    <dbReference type="STRING" id="273057.SSO1659"/>
    <dbReference type="PaxDb" id="273057-SSO1659"/>
    <dbReference type="EnsemblBacteria" id="AAK41873">
        <property type="protein sequence ID" value="AAK41873"/>
        <property type="gene ID" value="SSO1659"/>
    </dbReference>
    <dbReference type="GeneID" id="1454652"/>
    <dbReference type="KEGG" id="sso:SSO1659"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1709"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="InParanoid" id="Q97XQ0"/>
    <dbReference type="PhylomeDB" id="Q97XQ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF22">
        <property type="entry name" value="TNPB-LIKE PROTEIN MJ1635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="188"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="309"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="422" mass="49943" checksum="14AEEF9BD0926E15" modified="2001-10-01" version="1">MLKNLRIRKFEPEEEYVYFTYSIKNSEREKSKELIKEYRTLLQKAIDYLWSLTKIQVRKKNGNYKITLPKKREVYKPLRDELEKINHLASHYVDKAINNAFSIITSWRKRAIKGRASIEKPTLKKAYVKVKSTLRKVVGESVRITVRPHEYITFSWSKSWFSRRVRELELGEPIIKEEKVYLPFRYKLPWATPVNFLAIDSNLYTLDAYDGEKFVTISLKQLYSLKYSMEVKRAKVQSFASKHTKRGRELLRKYSHRERNRVLDFVHKFVNTLLDLYPVTFFAVEKLDKESMFKDANDSLSRKISRTVWGSIHKVLEYKAPLYGSFVKEVNPYLTSRSCPRCGFVSRKVGKTFECERCGFKLDRQLNASLNIYLKMCGFPHIRDVPRVWVGVIPLMGRRGMNVRDFGEAQGLRIDIKYHEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XR1</accession>
    <name>Q97XR1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1649</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90325">
        <property type="entry name" value="G90325"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923546.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XR1"/>
    <dbReference type="PaxDb" id="273057-SSO1649"/>
    <dbReference type="EnsemblBacteria" id="AAK41862">
        <property type="protein sequence ID" value="AAK41862"/>
        <property type="gene ID" value="SSO1649"/>
    </dbReference>
    <dbReference type="GeneID" id="1454641"/>
    <dbReference type="KEGG" id="sso:SSO1649"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1697"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077283_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XR1"/>
    <dbReference type="PhylomeDB" id="Q97XR1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="354" mass="40311" checksum="D10C2026BD83A87D" modified="2001-10-01" version="1">MTKELTREEYYKALEKAVNEVILSMTGTRKDVAKRLLLGAVVGRNATEIAQEAEMNYETVLNNLDKAAQVNLIEVVKKLVGDHPVLLIIDDTHDHKLYARAMPVSRNGAQIFYCRAHKRFESAIQLLVIGVKDLVNNQIYVIHIIAYIPRKVEEELKRRGEEVKFKTKIDALLEFLSSLSGLNVKSKVFDSWYVNSRTLQGNTVGELKSSARVVESGRSVPVSEFPQGEYLVEYLGTPIKLLVIDDYKGYGRRYFFSTDLNDTAEDIITTWENRWDIEVLIRELKALGLEGGSFLTWVRNSGFVALKALSLLVVQYFKYSTGLMLGAKRLARLIKSIYREAGGIKKLFKRRRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XR8</accession>
    <name>Q97XR8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90324">
        <property type="entry name" value="G90324"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923541.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XR8"/>
    <dbReference type="PaxDb" id="273057-SSO1640"/>
    <dbReference type="EnsemblBacteria" id="AAK41854">
        <property type="protein sequence ID" value="AAK41854"/>
        <property type="gene ID" value="SSO1640"/>
    </dbReference>
    <dbReference type="GeneID" id="7806660"/>
    <dbReference type="KEGG" id="sso:SSO1640"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1687"/>
    <dbReference type="eggNOG" id="arCOG01197">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130257_9_4_2"/>
    <dbReference type="InParanoid" id="Q97XR8"/>
    <dbReference type="PhylomeDB" id="Q97XR8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43449">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43449:SF1">
        <property type="entry name" value="POLYMERASE BETA NUCLEOTIDYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="119" mass="13681" checksum="686024CE46C0E554" modified="2001-10-01" version="1">MGKAKSALKSQVELVKVAIEFINDISNEIKIEDIYVVGSRARGDYLETSDIDLVIISRDFKGLRYIDRLEKLGKYLKARVEFFAFTPEEWNDSASLFVKQMKKEAKRLKDLAKEIGLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XV2</accession>
    <name>Q97XV2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3782 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1606</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90320">
        <property type="entry name" value="B90320"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923506.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XV2"/>
    <dbReference type="PaxDb" id="273057-SSO1606"/>
    <dbReference type="EnsemblBacteria" id="AAK41817">
        <property type="protein sequence ID" value="AAK41817"/>
        <property type="gene ID" value="SSO1606"/>
    </dbReference>
    <dbReference type="GeneID" id="1454596"/>
    <dbReference type="KEGG" id="sso:SSO1606"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1644"/>
    <dbReference type="eggNOG" id="arCOG01424">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_965117_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XV2"/>
    <dbReference type="PhylomeDB" id="Q97XV2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38753">
        <property type="entry name" value="SLR1441 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38753:SF1">
        <property type="entry name" value="SLR1441 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="30260" checksum="3DAFBAB5323FCCA9" modified="2001-10-01" version="1">MSGKIADEILNNPQLLSALADKIYDKLKDEIVIKKLEETIANVKALQEEVKRQGEAISSLQNTVNKHTEAITSLQEAVKKQGEAIQSLQEAVKSLQETVNKHTEAITSLQEAVKKQGEAIEGLQKAVRKLQRAVMKLSVEVGSFTNRAGKGMERTMLKLYKKALELHGVDPKRVVHGMIKDEEGVIEKGKVFEVDFYETNDYVYVFEIKNLADKGAYDQIIIRKKLFYTKYKDKKIKIFLVANYVDKKVKKKLEEEGVEIIASHIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XV4</accession>
    <name>Q97XV4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8462</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90319">
        <property type="entry name" value="H90319"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XV4"/>
    <dbReference type="STRING" id="273057.SSO8462"/>
    <dbReference type="PaxDb" id="273057-SSO8462"/>
    <dbReference type="EnsemblBacteria" id="AAK41815">
        <property type="protein sequence ID" value="AAK41815"/>
        <property type="gene ID" value="SSO8462"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8462"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1642"/>
    <dbReference type="eggNOG" id="arCOG03768">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2802447_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XV4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009321">
        <property type="entry name" value="DUF973"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06157">
        <property type="entry name" value="DUF973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="67" mass="7374" checksum="FDD95FCD4FD6460C" modified="2001-10-01" version="1">MAILVDTMLEVWPKGLLLNAPVKLNPANNNVTVIFPPLSGMIPKNNYNLILTLSNGQSIKIVVIYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XY2</accession>
    <name>Q97XY2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1574</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90316">
        <property type="entry name" value="D90316"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XY2"/>
    <dbReference type="STRING" id="273057.SSO1574"/>
    <dbReference type="PaxDb" id="273057-SSO1574"/>
    <dbReference type="EnsemblBacteria" id="AAK41787">
        <property type="protein sequence ID" value="AAK41787"/>
        <property type="gene ID" value="SSO1574"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1574"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1611"/>
    <dbReference type="eggNOG" id="arCOG01506">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077650_5_0_2"/>
    <dbReference type="InParanoid" id="Q97XY2"/>
    <dbReference type="PhylomeDB" id="Q97XY2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3480.10">
        <property type="entry name" value="TorD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020945">
        <property type="entry name" value="DMSO/NO3_reduct_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036411">
        <property type="entry name" value="TorD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050289">
        <property type="entry name" value="TorD/DmsD_chaperones"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34227">
        <property type="entry name" value="CHAPERONE PROTEIN YCDY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34227:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE CHAPERONE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02613">
        <property type="entry name" value="Nitrate_red_del"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89155">
        <property type="entry name" value="TorD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="135" mass="16005" checksum="34B4904D4BC24BBF" modified="2001-10-01" version="1">MAESNYDKVATEFTSCFINDYKHVKCPPYESWYRERTVYGISVQRVLEEYIKYGIYPKKQLADHISTELEFTSFLLFVEQEDEARKFIKEHIVSWVPKLIEDILANSKGEYTKLLGIALKQFLDYTIQTIFVVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y06</accession>
    <name>Q97Y06_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial transposase ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1548</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90313">
        <property type="entry name" value="F90313"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y06"/>
    <dbReference type="STRING" id="273057.SSO1548"/>
    <dbReference type="PaxDb" id="273057-SSO1548"/>
    <dbReference type="EnsemblBacteria" id="AAK41765">
        <property type="protein sequence ID" value="AAK41765"/>
        <property type="gene ID" value="SSO1548"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1548"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1585"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1302673_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y06"/>
    <dbReference type="PhylomeDB" id="Q97Y06"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="1">
        <location>
            <begin position="130"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="251" mass="29460" checksum="8969FD831DC239B3" modified="2001-10-01" version="1">MLWKEKDVRGKKTDFYDAEKLENMVNKAREYVNNPLKELVSLYLFMKDVQTKQENRLRRALFLVSDEEKLSEERLEKFSKGDFSDVKLYQLEYTGTVLREIQVLARTLLQTREELKEVRKMIEGNVPQDHVLLTIPGIGKLAAGIIIGIVGDIKRFPRPESFVAYCGLDPVVERSGRIEVRKGISKRGNKYLRSLFYFLAETQYSRNPTLLKFYEAHKDKLKGRKLYTALARKLARIVWSVWYNNKPYEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y76</accession>
    <name>Q97Y76_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1463</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90304">
        <property type="entry name" value="D90304"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989151.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y76"/>
    <dbReference type="FunCoup" id="Q97Y76">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1463"/>
    <dbReference type="PaxDb" id="273057-SSO1463"/>
    <dbReference type="EnsemblBacteria" id="AAK41691">
        <property type="protein sequence ID" value="AAK41691"/>
        <property type="gene ID" value="SSO1463"/>
    </dbReference>
    <dbReference type="GeneID" id="1454470"/>
    <dbReference type="KEGG" id="sso:SSO1463"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1495"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_15_12_2"/>
    <dbReference type="InParanoid" id="Q97Y76"/>
    <dbReference type="PhylomeDB" id="Q97Y76"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="364"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="417" mass="45078" checksum="CBF8D62B18551502" modified="2001-10-01" version="1">MSEKKLLTFWQALFIGLGNIIGAGIFVMAGSTISVAGPGAIIAFIFTAIFAMSIGLNSAELSSIFTDVEGGVYSFTLKTLGEMSGFLVGWFRVISYVIGGAATALGFSGYLLLFGLPKTLYYTLAVILIVILLVLDYFGIGTVAEVDTIFVIINISSLILFSLTIPIIVGIKVHNFVPLFPHGIQGVLTTSNLAFFSYSGFNTIVTLTPSTKNGEKVIPRAIVMSLIITSIIYISVIFSMVDAMPWYYYGQTSAPLSLALSQIHAPTWLFLITSISAILATISVTLSIIVASERTLRQLSRDFQLPVKHELLVVGGIMLASLLLGNVEAIALASNFGIIFSYMLTGLEVIIVRRKGLRGSFRSPAFPVIQVFAIILSALFMISLGLNSLEIGVVTLFIGILVYETLREVKAHENRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y78</accession>
    <name>Q97Y78_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99304">
        <property type="entry name" value="B99304"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y78"/>
    <dbReference type="PaxDb" id="273057-SSO1461"/>
    <dbReference type="EnsemblBacteria" id="AAK41689">
        <property type="protein sequence ID" value="AAK41689"/>
        <property type="gene ID" value="SSO1461"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1461"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1493"/>
    <dbReference type="eggNOG" id="arCOG07234">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2127951_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y78"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="116" mass="13021" checksum="154BEF54B6806E46" modified="2001-10-01" version="1">MIVMKVSNMIYIIISIVLAYIMQIFVLYPFTAIVVGVPLGLLSRKYSMIGSFLIGFLSSLSLYLIYPIDGVSRMAEIIGRLINANPFLAILLYPLIYGTISLISALLFYYIIRVSK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-25" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YJ8</accession>
    <name>THIC1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphomethylpyrimidine synthase 1</fullName>
            <ecNumber evidence="1">4.1.99.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Hydroxymethylpyrimidine phosphate synthase 1</fullName>
            <shortName evidence="1">HMP-P synthase 1</shortName>
            <shortName evidence="1">HMP-phosphate synthase 1</shortName>
            <shortName evidence="1">HMPP synthase 1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thiamine biosynthesis protein ThiC 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiC1</name>
        <name type="synonym">thiC-1</name>
        <name type="ordered locus">SSO1324</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + CO + 5'-deoxyadenosine + formate + L-methionine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:24840"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.99.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.17" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90288">
        <property type="entry name" value="B90288"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923318.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YJ8"/>
    <dbReference type="SMR" id="Q97YJ8"/>
    <dbReference type="FunCoup" id="Q97YJ8">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1324"/>
    <dbReference type="PaxDb" id="273057-SSO1324"/>
    <dbReference type="EnsemblBacteria" id="AAK41561">
        <property type="protein sequence ID" value="AAK41561"/>
        <property type="gene ID" value="SSO1324"/>
    </dbReference>
    <dbReference type="GeneID" id="1454340"/>
    <dbReference type="KEGG" id="sso:SSO1324"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1325"/>
    <dbReference type="eggNOG" id="arCOG02741">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013181_2_2_2"/>
    <dbReference type="InParanoid" id="Q97YJ8"/>
    <dbReference type="PhylomeDB" id="Q97YJ8"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070284">
        <property type="term" value="F:phosphomethylpyrimidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="entry name" value="Radical SAM ThiC family, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009895">
        <property type="entry name" value="PRK13352.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557:SF1">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="THIAMINE BIOSYNTHESIS PROTEIN THIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01114">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00113">
        <property type="entry name" value="Radical_SAM_Phosphomethylpyrim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000152874" description="Phosphomethylpyrimidine synthase 1">
        <location>
            <begin position="1"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="184"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="225"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00089"/>
        </source>
    </evidence>
    <sequence length="433" mass="47831" checksum="036D1E66D85FD24E" modified="2001-10-01" version="1">MAIIEEARSGQITDEMKEISKLEGIPVEKVRNRISECKIILIRNAKYPSKKLVPIGKGLTTKVNINIGTSDEVVNLQMELEKVKVANRWGDTLMDLSTGGDLDDIRREIIRASELPVGTVPVYQVFIESFKKKSGGAYFTEDELLSTVEKHLKDGVAFMTIHAGITKELAIRALKSNRIIPIVSRGGDMIAGWMIHNNSENPYRKHWGYLLEMFKEYDAVISLGDALRPGATGDAHDEFQVGELLETARLVKSALKEGVQVMVEGPGHVPLNEIAWDVKLMKKLTGGVPYYVLGPLPIDVGAPYDHIASAIGAAISSAAGADLLCYLTPAEHLGLPTVKQVEEGAIAYRIAAHAGDVVKLGRRVRKWDDEVSYYRGKLEWDNMISRLIDPQRAYQVYTQFGIPKVKACTMCGGYCPMMWAMDQVRKIGSSSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-10-02" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YN5</accession>
    <name>Q97YN5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1289</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90283">
        <property type="entry name" value="H90283"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YN5"/>
    <dbReference type="STRING" id="273057.SSO1289"/>
    <dbReference type="PaxDb" id="273057-SSO1289"/>
    <dbReference type="EnsemblBacteria" id="AAK41527">
        <property type="protein sequence ID" value="AAK41527"/>
        <property type="gene ID" value="SSO1289"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1289"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1288"/>
    <dbReference type="eggNOG" id="arCOG03167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041527_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YN5"/>
    <dbReference type="PhylomeDB" id="Q97YN5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025420">
        <property type="entry name" value="DUF4143"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295:SF19">
        <property type="entry name" value="ARCHAEAL ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13635">
        <property type="entry name" value="DUF4143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="77"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4143" evidence="2">
        <location>
            <begin position="259"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="53318" checksum="7A96C0FC82719ADB" modified="2001-10-01" version="1">MRNSLLVFTLDCQVKIDSKTVDCQTNIDSFLTPNSNLIIVEVEEIKRILLDQESFLNDKLRKERIITRHYNYSITYNNAYLITGPRRAGKSIFTVQLTKGKEILRVDFDDERLSGIKANELNKFLEAGYQIKKKIEVIILDEIQNVKGWELFVSRIREIYPVIVTGSNARLMSSEMATYLTGRHMDFLVFPFSFKEYLRYKDVKVEETTRGIARLKEELRNYLQQGGFPETFKFDKNEYLRNLYSDIITRDVIIRCKTRKDVKGLADFLLENIGKEVSTRRVGNAFNLSHQSVENYFYCLSNAYLFLFVKRFTGKSLERYTLPRKVYVIDTGLFNTYKRLQMGKMIENLVFLELYKRYGQEVYYYSNGNAEVDFIIKDKFAIQVTYDEGGIDDREYKGLVRFSERFKDYKLIIITWDREGKEIRNGKEITLIPLWKFLVKERFAGEMIGKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YR1</accession>
    <name>Q97YR1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90280">
        <property type="entry name" value="C90280"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991891.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YR1"/>
    <dbReference type="FunCoup" id="Q97YR1">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1260"/>
    <dbReference type="PaxDb" id="273057-SSO1260"/>
    <dbReference type="EnsemblBacteria" id="AAK41498">
        <property type="protein sequence ID" value="AAK41498"/>
        <property type="gene ID" value="SSO1260"/>
    </dbReference>
    <dbReference type="GeneID" id="1454277"/>
    <dbReference type="KEGG" id="sso:SSO1260"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1255"/>
    <dbReference type="eggNOG" id="arCOG05318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084161_2_0_2"/>
    <dbReference type="InParanoid" id="Q97YR1"/>
    <dbReference type="PhylomeDB" id="Q97YR1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11613">
        <property type="entry name" value="SAF_AH_GD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013974">
        <property type="entry name" value="SAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044144">
        <property type="entry name" value="SAF_UxaA/GarD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052172">
        <property type="entry name" value="UxaA_altronate/galactarate_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30536:SF5">
        <property type="entry name" value="ALTRONATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30536">
        <property type="entry name" value="ALTRONATE/GALACTARATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08666">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00858">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAF" evidence="2">
        <location>
            <begin position="11"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="99" mass="11181" checksum="4C06F21F7F7DDDDD" modified="2001-10-01" version="1">MPYFLIHNKNDNVGVAIADIKTGQEVEGIYIEDMTQGPKIKAISDIPLGHKIALKDIRQSDIVIKYGRPIGSAIKDIKVGEHVHVHNIRSNRWGKWKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YS0</accession>
    <name>Q97YS0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISC1359</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1245</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90279">
        <property type="entry name" value="A90279"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923271.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YS0"/>
    <dbReference type="STRING" id="273057.SSO1245"/>
    <dbReference type="PaxDb" id="273057-SSO1245"/>
    <dbReference type="EnsemblBacteria" id="AAK41488">
        <property type="protein sequence ID" value="AAK41488"/>
        <property type="gene ID" value="SSO1245"/>
    </dbReference>
    <dbReference type="GeneID" id="1454267"/>
    <dbReference type="KEGG" id="sso:SSO1245"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1243"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067987_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YS0"/>
    <dbReference type="PhylomeDB" id="Q97YS0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033541">
        <property type="entry name" value="transpos_ISH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="91"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="350" mass="40583" checksum="29A5C4FEE0DC6091" modified="2001-10-01" version="1">MKHENTTKPAKFNRDFARSALKIIYSILTKILFLEELLSALLKASGSYLSRLGKDGRRALRKLNAVQVEDVRDALRKMGRMTLRGVRDKRVAVDFHAIPQYHANKSFLSRIKPTKGTSWGLVQAAIFLLGRTRSFLDVIPVTVKNVAEGFKAVMEVIVKELEEDKLRLVMVFADREFAVNEVIRYLLELGLDFVISAKAQMYKKYKGMLQDVDVSFGGVRYTGFLCVRHGTGAYLIILRKEDGKIIAFLVRREIDLHDAIVLAEMYRERWGIENAFRSLEEFRIRTRTCDVRKELVLVLLSYLLLNVWFLIRSWRKVKLWEFSVSLSNLLDREVRVEQGRVFREVKTSFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YU1</accession>
    <name>Q97YU1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Second ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1216</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90275">
        <property type="entry name" value="H90275"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923254.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YU1"/>
    <dbReference type="STRING" id="273057.SSO1216"/>
    <dbReference type="PaxDb" id="273057-SSO1216"/>
    <dbReference type="EnsemblBacteria" id="AAK41463">
        <property type="protein sequence ID" value="AAK41463"/>
        <property type="gene ID" value="SSO1216"/>
    </dbReference>
    <dbReference type="GeneID" id="1454242"/>
    <dbReference type="KEGG" id="sso:SSO1216"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1216"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="InParanoid" id="Q97YU1"/>
    <dbReference type="PhylomeDB" id="Q97YU1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF22">
        <property type="entry name" value="TNPB-LIKE PROTEIN MJ1635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="188"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="309"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="422" mass="50054" checksum="3736EAE1681642DB" modified="2001-10-01" version="1">MLKNLRIGKFEPEEEYVHFTYSIKNSEREKSKELIKEYRTLLQKAIDYLWSLTKIQVRKKNGNYKITLPKKKEVYKPLREELEKINHLASHYVDKAINDAFSILKSWRKRAIKGRASIEKPRVKKAYVRIKTTLRKVVGESVRITVRPHEYITFPWSKSWFSRRVRELELGEPIIKEEKVYLPFRYKLPWVTPVNFLAIDSNLYTLDAYDGEKFVTISLKQLYSLKYSMEVKRAKVQSFASKHTKRGRELMRKYSHRERNRVLDFVHKFVNALLDLYPMTFFAVEKLDKEGMFVDANESLSRKISRTVWRSIHRVLKYKAPLYGSFVKEVNPYLTSRSCPRCGFVSRKVGKTFECERCGFKLDRQLNASLNIYLKMCGFPHIREIARVWVGVIPLMGRRGMNVRDFGEAQGLRINIKYHEIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z33</accession>
    <name>Q97Z33_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HAD family hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90263">
        <property type="entry name" value="D90263"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989739.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z33"/>
    <dbReference type="STRING" id="273057.SSO1106"/>
    <dbReference type="PaxDb" id="273057-SSO1106"/>
    <dbReference type="EnsemblBacteria" id="AAK41363">
        <property type="protein sequence ID" value="AAK41363"/>
        <property type="gene ID" value="SSO1106"/>
    </dbReference>
    <dbReference type="GeneID" id="1454142"/>
    <dbReference type="KEGG" id="sso:SSO1106"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1103"/>
    <dbReference type="eggNOG" id="arCOG05919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1623454_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z33"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="19008" checksum="E71E660F46728EC1" modified="2001-10-01" version="1">MVNFAVWLDGVILKIDLTDSLYKIYKGDQISLPITYEVNDDWIKVYDRIREANLAILSPYDEITTKAILERINLKPTYVIANRGKTKPSREPYRILIEVTGWDPLQVVTLASSPLDLLSARFFDSRIKVICVKRYQDCSKYSPFLYSENLDDAISSLKRLKIIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-10" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z79</accession>
    <name>IF2A_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 2 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">aIF2-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">eIF-2-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif2a</name>
        <name type="synonym">aif2a</name>
        <name type="ordered locus">SSO1050</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="13366" last="13366">
            <title>Cryo-EM study of start codon selection during archaeal translation initiation.</title>
            <authorList>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Monestier A."/>
                <person name="Larquet E."/>
                <person name="Cladiere L."/>
                <person name="Klaholz B.P."/>
                <person name="Schmitt E."/>
                <person name="Mechulam Y."/>
            </authorList>
            <dbReference type="PubMed" id="27819266"/>
            <dbReference type="DOI" id="10.1038/ncomms13366"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (5.34 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="3">Assembles into a translation intitiation complex with leadered or leaderless mRNA, 30S ribosomes and tRNA(Met)i (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-9010365">
            <id>Q97Z79</id>
        </interactant>
        <interactant intactId="EBI-9010337">
            <id>Q980A5</id>
            <label>eif2g</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>2</experiments>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-alpha family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAK41314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="C90257"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="EMDB" id="EMD-8148"/>
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    <dbReference type="SASBDB" id="Q97Z79"/>
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        <property type="interactions" value="306"/>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_033458_0_2_2"/>
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        <property type="organism ID" value="6163"/>
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        <property type="term" value="F:ribosome binding"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
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    <sequence length="266" mass="30382" checksum="A94E66BBE2931272" modified="2001-10-01" version="1">MIYSRSKLPSEGEILIATVKQVFDYGSYVSLDEYGGLQAFLPWSEVSSKWVKNIRDVLKENRKVIVKVIRVDRRKGTVDVSLKKVTDDERRKKNLQWKKIQRLDKILELVSQKLKLSEKDAWEQVAWKLEAKYGDPITAIEKAVKEGEKILIDAGVPEIWVKPLLEEASKHAEERKVKMSGLITVRTNEPLGVEKIKEVISKALENIEQDYESLLNIKIYTIGAPRYRVDVVGTNPKEASEALNQIISNLIKIGKEENVDISVVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z93</accession>
    <name>Q97Z93_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine/threonine protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1038</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90255">
        <property type="entry name" value="E90255"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z93"/>
    <dbReference type="STRING" id="273057.SSO1038"/>
    <dbReference type="PaxDb" id="273057-SSO1038"/>
    <dbReference type="EnsemblBacteria" id="AAK41300">
        <property type="protein sequence ID" value="AAK41300"/>
        <property type="gene ID" value="SSO1038"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1038"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1032"/>
    <dbReference type="eggNOG" id="arCOG01182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095575_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z93"/>
    <dbReference type="PhylomeDB" id="Q97Z93"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052396">
        <property type="entry name" value="Meiotic_Drive_Suppr_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37171">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE YRZF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37171:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE YRZF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="30185" checksum="5979FDCBB3EE0685" modified="2001-10-01" version="1">MGWRRWKIILYKREKGIRCRDNSQKEYSSKGEVQYRELLATKHGRSTNNEILEENSNLVKISCFIYPTYSKEVEEELRKAGFDYAYSFGQKNLGSLKVLGKGKTGIVVLVEPYKVLKIRRTDAPKETLEIEAKLQIKAGEEVAPRVYDYGKNFILMEYIHGRNLMKNERRETIIDLLERARILEEKLIEHKELSRPWKNVIVSYNRTYIIDYDSATIKEKPRNVTKLLSNYLNRRDLGVKYSKSEITFDQLLSQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z95</accession>
    <name>Q97Z95_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MacB-like periplasmic core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90255">
        <property type="entry name" value="C90255"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z95"/>
    <dbReference type="STRING" id="273057.SSO1035"/>
    <dbReference type="PaxDb" id="273057-SSO1035"/>
    <dbReference type="EnsemblBacteria" id="AAK41298">
        <property type="protein sequence ID" value="AAK41298"/>
        <property type="gene ID" value="SSO1035"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1035"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1028"/>
    <dbReference type="eggNOG" id="arCOG08556">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065514_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z95"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="326"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="41404" checksum="5AA6E314E99D41BA" modified="2001-10-01" version="1">MLNMLKSIFKQFLTRNSIRDLVIFLLILLLFLPILHLFFFALFSSLTIYQVRATSYSNLSVVSIAGNNNTLVINQLERLFGNNSLAPFGYIYIGARLGGATVEAIAINNLSLAKKIFPISSMIAIGRYPEYGVYLVAGKMNQLNLQLGENVSVYMGKNIISLRVVGILYNAFFSAEGFAILPLTNINYTINSLDNVIILKDNYTALNEFKAFLSNTKYEGLPLSDWYSITRFGSSFTFSYFQLNSLRTTSEIVETIYILISLLLIFRIRSRFSNVLALFIINGVDEKRSFKLLILPFLVIGIIPLIVYIAYYYLYDVYWYPLSVVYPFIAGLIGLLVSSILIEYYTVFSAYSNQHKFLKVRSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZA7</accession>
    <name>Q97ZA7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Formate hydrogenlyase subunit 5 (HycE)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hycE</name>
        <name evidence="3" type="ordered locus">SSO1023</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90253">
        <property type="entry name" value="G90253"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989217.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZA7"/>
    <dbReference type="SMR" id="Q97ZA7"/>
    <dbReference type="STRING" id="273057.SSO1023"/>
    <dbReference type="PaxDb" id="273057-SSO1023"/>
    <dbReference type="EnsemblBacteria" id="AAK41286">
        <property type="protein sequence ID" value="AAK41286"/>
        <property type="gene ID" value="SSO1023"/>
    </dbReference>
    <dbReference type="GeneID" id="7797808"/>
    <dbReference type="KEGG" id="sso:SSO1023"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1014"/>
    <dbReference type="eggNOG" id="arCOG01547">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057086_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZA7"/>
    <dbReference type="PhylomeDB" id="Q97ZA7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052197">
        <property type="entry name" value="ComplexI_49kDa-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43485:SF1">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43485">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="2">
        <location>
            <begin position="172"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="391" mass="44254" checksum="1B61A0EB84022346" modified="2001-10-01" version="1">MKYYKWTQKGEGRKIGKIGDYCLYEKTITEEKCEENKPNITQTYGSFKFIYGPSAGGLLETIKFIITTNGEKILGIDAEVYKNREIVISGLTVDDALLRVERINAPFSASHTISFLLAVEDSLELEQDYPTQLKRIAEIELERIRNHLFVISRLTETTSLNVPTYHLLHLVEKVNRLIGKMCGHRYFFGVNAINGVNCDFGNLLRIIDITKEFKQIFDGLLESRIFIDRLQENGKIIDENSIGPAARAAGLAYDARKDFKALPYEDLGFRTVITQEADSFGRFLVRGMEIIESAKILVELHDEIKNSNNERGKNHKQGGGEGLARVESPSGDLAYYVKLNNGIIDSVSLLTPSQVNLNLFLKSVINTIFTDFQFNWESFGIWVSEIGVMLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZG2</accession>
    <name>Q97ZG2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase</fullName>
            <ecNumber evidence="9">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">tRNA wyosine derivatives biosynthesis protein Taw1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">taw1</name>
        <name evidence="11" type="ordered locus">SSO0956</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the wyosine derivatives biosynthesis pathway that catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine (imG-14) on guanosine-37 of tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TYW1 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="9"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90246">
        <property type="entry name" value="G90246"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZG2"/>
    <dbReference type="STRING" id="273057.SSO0956"/>
    <dbReference type="PaxDb" id="273057-SSO0956"/>
    <dbReference type="EnsemblBacteria" id="AAK41230">
        <property type="protein sequence ID" value="AAK41230"/>
        <property type="gene ID" value="SSO0956"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0956"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.952"/>
    <dbReference type="eggNOG" id="arCOG04174">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007952_3_0_2"/>
    <dbReference type="InParanoid" id="Q97ZG2"/>
    <dbReference type="PhylomeDB" id="Q97ZG2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01921">
        <property type="entry name" value="TYW1_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023993">
        <property type="entry name" value="TYW1_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03972">
        <property type="entry name" value="rSAM_TYW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="60"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK41230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="365" mass="42196" checksum="7795D22A24751429" modified="2001-10-01" version="1">MCDRMYNMQSNLRIDTVSEIFKELQKEKYHIIGTHSAYKKCHWTHSALVANRSCYKGKFYGIESHRCVQMTPTAAWCWFRCVHCWRLEPEDVGLEWDDTKMPAYDDPEYIVERSIEEHRKAVSGYLGRNDVSIQKVKDAMKPAHVAISLTGEPTLYEKLGDLIKEYHKRGMTTFLVTSGVRPDILASLEEEPTQLFVSLQAPNEIKHKMINRPVVANSWQLVMKTLEILPSFSSPTVIRFTMIKGYNMSEQDAKEFAKLMEIAMPTYIEIKAYMHVGPSTYRLSRDAMPKHKEIREFAKILGQYTGYKILSEHVPSRIVLLSKLDKPIKIGNAWNEKWNWSTIDMEDDMSGEYKEAEQGCTEGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZI0</accession>
    <name>Q97ZI0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SUF system FeS cluster assembly SufBD core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0928</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99244">
        <property type="entry name" value="A99244"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992360.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZI0"/>
    <dbReference type="STRING" id="273057.SSO0928"/>
    <dbReference type="PaxDb" id="273057-SSO0928"/>
    <dbReference type="EnsemblBacteria" id="AAK41208">
        <property type="protein sequence ID" value="AAK41208"/>
        <property type="gene ID" value="SSO0928"/>
    </dbReference>
    <dbReference type="GeneID" id="1455175"/>
    <dbReference type="KEGG" id="sso:SSO0928"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.928"/>
    <dbReference type="eggNOG" id="arCOG01715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_736928_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZI0"/>
    <dbReference type="PhylomeDB" id="Q97ZI0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990229">
        <property type="term" value="C:iron-sulfur cluster assembly complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43575">
        <property type="entry name" value="PROTEIN ABCI7, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43575:SF1">
        <property type="entry name" value="PROTEIN ABCI7, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="1">
        <location>
            <begin position="138"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="389" mass="43858" checksum="987E6D38B74CCD07" modified="2001-10-01" version="1">MQWLKLGVVDIQLAKKVINENFTQDKGEREKAFSLYLSKPYQLIHDSPTIKHYTEWDLYDSLNLTNISRQEVKYNDTANVIEIIDDTIKIPKNDIQIDDLLNDSIISSDEHKLVSLAYSLSRRITINNEGEYYVKHVLNKDKYFSPSHLIINVPNDKQIKVVYEVLNLSESSLITPIISVNIEDNSSLDFQFVNFSSDNSLLFSYIKANIKGTMHSSIFVNGNKMGHVQFSTRLEESSVSEFSSRAFGTHSNKIDVVNNVIHLGQKSVSNGFMKAISNDQAFTVVRGVATIDETAINSSTSIIGRSLVLGKDAKAVVSPMLEVKTGRVVMAKHSAAVSKIDENQIFYLQTRGLSRREAEGIIIRGFIIEEQDPETLKDRIEEILKSLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZK0</accession>
    <name>Q97ZK0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Soluble hydrogenase, small subunit</fullName>
            <ecNumber evidence="7">1.12.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO0906</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="1.12.-.-" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90241">
        <property type="entry name" value="E90241"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992330.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZK0"/>
    <dbReference type="STRING" id="273057.SSO0906"/>
    <dbReference type="PaxDb" id="273057-SSO0906"/>
    <dbReference type="EnsemblBacteria" id="AAK41188">
        <property type="protein sequence ID" value="AAK41188"/>
        <property type="gene ID" value="SSO0906"/>
    </dbReference>
    <dbReference type="GeneID" id="1455155"/>
    <dbReference type="KEGG" id="sso:SSO0906"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.908"/>
    <dbReference type="eggNOG" id="arCOG00082">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027686_1_1_2"/>
    <dbReference type="InParanoid" id="Q97ZK0"/>
    <dbReference type="PhylomeDB" id="Q97ZK0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004760">
        <property type="term" value="F:L-serine-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019265">
        <property type="term" value="P:glycine biosynthetic process, by transamination of glyoxylate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000251">
        <property type="entry name" value="Soluble hydrogenase, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152">
        <property type="entry name" value="AMINOTRANSFERASE CLASS V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152:SF39">
        <property type="entry name" value="SOLUBLE HYDROGENASE, SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="6">
        <location>
            <begin position="48"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="373" mass="41688" checksum="1F0015D14A48E4FB" modified="2001-10-01" version="1">MMLIPGPVNVPYSVLQASLNLVNHRSEKFRETVKTLEFLMNKHFGSTRVALLSGSGTLAVESMVYSLVKREEKVITFPYGEFGHRLEESLIRRGAKVISYQKKLGESFTVEEIKKAIEENKDATTVALVHNETSTGIAFRDLKKIADVVKGVGLKLLVDSVSGFAAYPLYVNQWKIDCVVTGSQKALASIPGVGFAALSEEGVNELVESDLPSYLDLSFHLKFQDKGETPFTPTVGAFFASRRAAELLDKEGIENRWKRHEACARYLREVMLEMGFKLLGNDTNFSNTVVAAFPPIPLDTFMSELKKRNIEISKGMGELRDKIVRIGILGVVDDRAIRRLVGSMGEILKKNVEFEVPRECKLPEELKVEVLWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZL1</accession>
    <name>Q97ZL1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myo-inositol-1-phosphate synthase GAPDH-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0886</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the myo-inositol 1-phosphate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90239">
        <property type="entry name" value="B90239"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZL1"/>
    <dbReference type="FunCoup" id="Q97ZL1">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0886"/>
    <dbReference type="PaxDb" id="273057-SSO0886"/>
    <dbReference type="EnsemblBacteria" id="AAK41169">
        <property type="protein sequence ID" value="AAK41169"/>
        <property type="gene ID" value="SSO0886"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0886"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.890"/>
    <dbReference type="eggNOG" id="arCOG04213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050011_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZL1"/>
    <dbReference type="PhylomeDB" id="Q97ZL1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052199">
        <property type="entry name" value="MIPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013021">
        <property type="entry name" value="Myo-inos-1-P_Synthase_GAPDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43125">
        <property type="entry name" value="INOSITOL-3-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43125:SF1">
        <property type="entry name" value="INOSITOL-3-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01658">
        <property type="entry name" value="Inos-1-P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015578">
        <property type="entry name" value="Myoinos-ppht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myo-inositol-1-phosphate synthase GAPDH-like" evidence="2">
        <location>
            <begin position="192"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="360" mass="39378" checksum="2F3B2B3CD576AA9F" modified="2001-10-01" version="1">MMIRVAIAGLGNCASMLIQGIEYYKSKGDNYYEGLITPVIGGYKITDIEVVAAFDVSKNKIGKDLAEAVFQPPNITPKIVNMEKKGVKVSAGPVLDGVAQHMTNVFNPTYDGTLEKALDELKSSKAEILVNLLPVGSENATRTYANIALMAGTAFINAIPVFIASDPSGYFPNKFKEKNLPLAGDDIKSQLGATIFHRSITSLFRLRGVKVEETYQLNVGGNTDFLNMKTEERLISKRISKTEAVTSTLDNGEVIKSEGKIRIGPSDYVPFLGNTKVAYIYVKGSAFAGMPIKVEASLEVDDKANCAAVLVDVIRAVKVALDRKIGGPLEKVSAFYFKHPPIQAKDDEEAYRWFKEFIEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZQ7</accession>
    <name>Q97ZQ7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2250 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0668</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90214">
        <property type="entry name" value="D90214"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991212.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZQ7"/>
    <dbReference type="STRING" id="273057.SSO0668"/>
    <dbReference type="PaxDb" id="273057-SSO0668"/>
    <dbReference type="EnsemblBacteria" id="AAK40971">
        <property type="protein sequence ID" value="AAK40971"/>
        <property type="gene ID" value="SSO0668"/>
    </dbReference>
    <dbReference type="GeneID" id="1454938"/>
    <dbReference type="KEGG" id="sso:SSO0668"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.668"/>
    <dbReference type="eggNOG" id="arCOG05388">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2140235_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZQ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="13203" checksum="94A4455241E3FAEF" modified="2001-10-01" version="1">MDELMKKILIHIYKYGPDNPWYMARRLLGESGWIAKYDMAQIEEACKKLEELGYLIRFKGSLKKSVTSSIKPWLKVKARELDHKPNGIYYDLSKEGRKVASLLYKEYRKENG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZS3</accession>
    <name>Q97ZS3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0506</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90196">
        <property type="entry name" value="C90196"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991402.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZS3"/>
    <dbReference type="STRING" id="273057.SSO0506"/>
    <dbReference type="PaxDb" id="273057-SSO0506"/>
    <dbReference type="EnsemblBacteria" id="AAK40826">
        <property type="protein sequence ID" value="AAK40826"/>
        <property type="gene ID" value="SSO0506"/>
    </dbReference>
    <dbReference type="GeneID" id="1455636"/>
    <dbReference type="KEGG" id="sso:SSO0506"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.501"/>
    <dbReference type="eggNOG" id="arCOG07281">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_846275_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZS3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="38378" checksum="9B5F38CD53BF45E3" modified="2001-10-01" version="1">MERIVELEEQIKKERNKEKRNTLYISLIQEYHNLYKKYKNTEFLDRALSIANKIEKKNPTVLNILGLIYLEKRRPDIAILTFKELLSNYNLNNEDKNVILYNLSLAYFQKGELLNAYNTLKGLVLNSRGEINVLSKKLLVKVCLALGEIRYIEEARELLEEFNIPSEELAIAYAALAKYYNDNKLLKKAKDIAIVLGNEKILADILSISDDEEDLRKAIQIYEKLGDHNSELKPLYKLSNKDPSLFAQILEKIGNLEDSKDKLVILYDIYRRTKILQFLKEAIKTAEKLQDYLFLARAYSELANYEEEVTNLRKAISFYEKYIEKSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZT6</accession>
    <name>Q97ZT6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phosphate transport system permease protein (PstA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pstA</name>
        <name evidence="7" type="ordered locus">SSO0491</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90194">
        <property type="entry name" value="E90194"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923006.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZT6"/>
    <dbReference type="FunCoup" id="Q97ZT6">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0491"/>
    <dbReference type="PaxDb" id="273057-SSO0491"/>
    <dbReference type="EnsemblBacteria" id="AAK40812">
        <property type="protein sequence ID" value="AAK40812"/>
        <property type="gene ID" value="SSO0491"/>
    </dbReference>
    <dbReference type="GeneID" id="1455622"/>
    <dbReference type="KEGG" id="sso:SSO0491"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.485"/>
    <dbReference type="eggNOG" id="arCOG00168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_2_0_2"/>
    <dbReference type="InParanoid" id="Q97ZT6"/>
    <dbReference type="PhylomeDB" id="Q97ZT6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051408">
        <property type="entry name" value="Phosphate_transprt_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42922">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN PSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42922:SF1">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN PSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="64"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="272" mass="29620" checksum="9F89194C6B455312" modified="2001-10-01" version="1">MRRKDTVIVSLITLLVILFLLPITFLLYTVVVNGSKVIISYGPRFFIDLPPNPGEGLGGIGPALEGSLYMVSLAVLISAPISIFTGVFLSFFERSKIASLIRASLELMVEFPTIVIGIVIFTILVVEIGLGLNGITAAMALAIVMLPYSTIQISEALRIPKKLYEEVAYSLGLTTWQVIKLVMYIGRRGIINGLLIGFAKIIGETAPIIFLTSSTANLYIKTFNTAVTGIPVMIYNYAFSGYSNLNEVAWGASFVLIILVTIIFVVVRKMAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZV6</accession>
    <name>Q97ZV6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helicase HerA central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90192">
        <property type="entry name" value="A90192"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922986.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZV6"/>
    <dbReference type="STRING" id="273057.SSO0469"/>
    <dbReference type="PaxDb" id="273057-SSO0469"/>
    <dbReference type="EnsemblBacteria" id="AAK40792">
        <property type="protein sequence ID" value="AAK40792"/>
        <property type="gene ID" value="SSO0469"/>
    </dbReference>
    <dbReference type="GeneID" id="7807413"/>
    <dbReference type="KEGG" id="sso:SSO0469"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.464"/>
    <dbReference type="eggNOG" id="arCOG00283">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_430008_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZV6"/>
    <dbReference type="PhylomeDB" id="Q97ZV6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051162">
        <property type="entry name" value="T4SS_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121:SF11">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJGR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase HerA central" evidence="1">
        <location>
            <begin position="164"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="582" mass="66756" checksum="7D29F258BD08D3F1" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZZ3</accession>
    <name>Q97ZZ3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein L-isoaspartate methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO0427</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99187">
        <property type="entry name" value="A99187"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922967.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZZ3"/>
    <dbReference type="FunCoup" id="Q97ZZ3">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0427"/>
    <dbReference type="PaxDb" id="273057-SSO0427"/>
    <dbReference type="EnsemblBacteria" id="AAK40752">
        <property type="protein sequence ID" value="AAK40752"/>
        <property type="gene ID" value="SSO0427"/>
    </dbReference>
    <dbReference type="GeneID" id="1455562"/>
    <dbReference type="KEGG" id="sso:SSO0427"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.419"/>
    <dbReference type="eggNOG" id="arCOG00978">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025402_0_1_2"/>
    <dbReference type="InParanoid" id="Q97ZZ3"/>
    <dbReference type="PhylomeDB" id="Q97ZZ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000529">
        <property type="entry name" value="tRNA (1-methyladenosine) methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049470">
        <property type="entry name" value="TRM61_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133:SF1">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017269">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="6">
        <location>
            <begin position="59"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="108"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR017269-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="256" mass="29043" checksum="3F35447F91A81C8A" modified="2001-10-01" version="1">MMPLNEGDPVVIWIDPKRVYISKLERGKRLDTDKGVVFYDNLIGLEYGTSLTLKTGVKAYLLKPTIQDLYSKGLKRPSQVLYPKDIGYILTTLSLNQVKTVIEAGTGSGFLTISLALSLSENAKIYTYDIREDMQKVAKFNANVLGVQDRIVFKLKDIREGIDEKEVDAIFLDMPDPWNAIPKLYESLKPSSTIIIFVPTVNQIEKTYFAMKNSGIIDIHVEELILREYQVKENAIRPRNIGIMHTGFIIRGRKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZZ9</accession>
    <name>Q97ZZ9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AAA family ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO0421</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90186">
        <property type="entry name" value="C90186"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZZ9"/>
    <dbReference type="FunCoup" id="Q97ZZ9">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0421"/>
    <dbReference type="PaxDb" id="273057-SSO0421"/>
    <dbReference type="EnsemblBacteria" id="AAK40746">
        <property type="protein sequence ID" value="AAK40746"/>
        <property type="gene ID" value="SSO0421"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0421"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.413"/>
    <dbReference type="eggNOG" id="arCOG01308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_12_2_2"/>
    <dbReference type="InParanoid" id="Q97ZZ9"/>
    <dbReference type="PhylomeDB" id="Q97ZZ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19529">
        <property type="entry name" value="RecA-like_VCP_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000007">
        <property type="entry name" value="AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000057">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000008">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF199">
        <property type="entry name" value="AAA FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="7">
        <location>
            <begin position="30"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="6">
        <location>
            <begin position="128"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="234"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="509"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK40746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="769" mass="86347" checksum="A90EBD42C0ED11AB" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q980E4</accession>
    <name>Q980E4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Long-chain-fatty-acid--CoA ligase (FadD-1)</fullName>
            <ecNumber evidence="3">6.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fadD-1</name>
        <name evidence="3" type="ordered locus">SSO0369</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90180">
        <property type="entry name" value="D90180"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990671.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980E4"/>
    <dbReference type="FunCoup" id="Q980E4">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0369"/>
    <dbReference type="PaxDb" id="273057-SSO0369"/>
    <dbReference type="EnsemblBacteria" id="AAK40699">
        <property type="protein sequence ID" value="AAK40699"/>
        <property type="gene ID" value="SSO0369"/>
    </dbReference>
    <dbReference type="GeneID" id="1455509"/>
    <dbReference type="KEGG" id="sso:SSO0369"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.363"/>
    <dbReference type="eggNOG" id="arCOG00857">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_2"/>
    <dbReference type="InParanoid" id="Q980E4"/>
    <dbReference type="PhylomeDB" id="Q980E4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="2">
        <location>
            <begin position="384"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="454" mass="51562" checksum="00422D21851371B3" modified="2001-10-01" version="1">MSLATLLYKWFKETPSKTFLIDKNQSLTYEQVTREVVYVASNISPGDTVVHIMLNSLQSVINYLAIFWAGGKAVAVDPQTSAEDLKFILEDSNPDLIVTDEEIYKREYDILKSYQVIVNKRGKDVFTKPYEYREDEVGLVYYYAGIVGRTMQVLHSANRVELNSATLYNSIGLKEIRGILTVPLAHVLGNSVLGITLEAGGKLYIMSKFNVDEAIESINKYNINYLSTVPTVYDYLNNEEKRKVDSLELCVSSAAPLYPSTVNTFKSKYGKDIVQQYGFTEGLILTFQPKELSGVISIGKPLPGVEIKIVDDKGNENNEGELWVKAPWLMLGYKDREETARVFNEGWLRTGDLVKIDEKGLLYFRGVKKRMLKFKGYPIFPRDLEEILKTHPMVEDVKVIGEDAGNLGQQPVALVKVKEHKAGIEDELLNYVNSRVAFYKRLKKVYVVDRIERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q980F1</accession>
    <name>Q980F1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mn2+-dependent serine/threonine protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90179">
        <property type="entry name" value="D90179"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990660.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980F1"/>
    <dbReference type="STRING" id="273057.SSO0361"/>
    <dbReference type="PaxDb" id="273057-SSO0361"/>
    <dbReference type="EnsemblBacteria" id="AAK40691">
        <property type="protein sequence ID" value="AAK40691"/>
        <property type="gene ID" value="SSO0361"/>
    </dbReference>
    <dbReference type="GeneID" id="1455501"/>
    <dbReference type="KEGG" id="sso:SSO0361"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.354"/>
    <dbReference type="eggNOG" id="arCOG01186">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090895_0_0_2"/>
    <dbReference type="InParanoid" id="Q980F1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="26113" checksum="BFD1288A34A1B22F" modified="2001-10-01" version="1">MFKNSYSQSRIIKKDNNIYVQKCYMTIFGIKWYFISTFFWNYPYVADPKERFVRELDFFLDNAWKDTVVVPKLIDIDAKELCITREFIEGDEINEENIEIMAKGLREIHEVGYVLGDTKISNFVIVDKSKLAVIDAEQSFKSNNLYYRAWDLLVFFLFLSYKMVNISHFQEVAKRFLDEYMPDKNIVKEFFDMKNINLMGLYPPTHLYVLKNLMSNFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q980F2</accession>
    <name>Q980F2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cysteine synthase B (CysM)</fullName>
            <ecNumber evidence="3">2.5.1.47</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cysM</name>
        <name evidence="3" type="ordered locus">SSO0360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90179">
        <property type="entry name" value="C90179"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990658.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980F2"/>
    <dbReference type="FunCoup" id="Q980F2">
        <property type="interactions" value="208"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0360"/>
    <dbReference type="PaxDb" id="273057-SSO0360"/>
    <dbReference type="EnsemblBacteria" id="AAK40690">
        <property type="protein sequence ID" value="AAK40690"/>
        <property type="gene ID" value="SSO0360"/>
    </dbReference>
    <dbReference type="GeneID" id="1455500"/>
    <dbReference type="KEGG" id="sso:SSO0360"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.352"/>
    <dbReference type="eggNOG" id="arCOG01430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_2"/>
    <dbReference type="InParanoid" id="Q980F2"/>
    <dbReference type="PhylomeDB" id="Q980F2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000082">
        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="2">
        <location>
            <begin position="16"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="297" mass="32623" checksum="380F348826D7857F" modified="2001-10-01" version="1">MSNPLHVFSNEQELLEGMWPTPLLKLRIGNDTWAKLEFYNPFSRSVKDRTAWFLFRQALLKNATHIVEATSGNTGIALASLSSIFGMRFTMFLPVVAPKVYKVFVKLLGGNVIEAGNSTNDVIPLVKKFAEMTNALNLDQFNNPINVIAHYETTAREIDEQLSSIGKKPSRIIATMGTGGHIAGIAKYFKEKYGNDVEIIGVQPSENSRIPGIKRQNGSNSLLRDVKIDRVVDVSLEEAIDGTISVARTSGILIGISAGATVAAYKKISDVSSTTVLIFPDDAFKYVDILEQTLKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q980N7</accession>
    <name>Q980N7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Adenylate cyclase, cyaB-type, putative (CyaB)</fullName>
            <ecNumber evidence="2">4.6.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cyaB</name>
        <name evidence="2" type="ordered locus">SSO0253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.6.1.1" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90167">
        <property type="entry name" value="A90167"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990516.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980N7"/>
    <dbReference type="STRING" id="273057.SSO0253"/>
    <dbReference type="PaxDb" id="273057-SSO0253"/>
    <dbReference type="EnsemblBacteria" id="AAK40592">
        <property type="protein sequence ID" value="AAK40592"/>
        <property type="gene ID" value="SSO0253"/>
    </dbReference>
    <dbReference type="GeneID" id="1455402"/>
    <dbReference type="GeneID" id="44129222"/>
    <dbReference type="KEGG" id="sso:SSO0253"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.247"/>
    <dbReference type="eggNOG" id="arCOG01723">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105244_2_0_2"/>
    <dbReference type="InParanoid" id="Q980N7"/>
    <dbReference type="PhylomeDB" id="Q980N7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07890">
        <property type="entry name" value="CYTH-like_AC_IV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008173">
        <property type="entry name" value="Adenylyl_cyclase_CyaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023577">
        <property type="entry name" value="CYTH_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00318">
        <property type="entry name" value="cyaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21028:SF2">
        <property type="entry name" value="CYTH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21028">
        <property type="entry name" value="SI:CH211-156B7.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01928">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01118">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51707">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYTH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="186" mass="21820" checksum="1B47C630B438C868" modified="2001-10-01" version="1">MIEKEVKIVVEYPSLEELRESLGKDMKLLDVEKQEDVYFNYVYRNFKITDEAVRIRINNKGIELTYKGPKLHSSLKAREEISITVNDLNKTIELLRKIGFYPVITIKKTRINYLDKSFIVSLDLVKDLGQFIEIEAINEISDQEIQNYTSNFIRKYKIKGKLTTKSYLELLIDKYEASSKSNSNTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q980T2</accession>
    <name>Q980T2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Archaeal Rqc2 homolog aRqcH</fullName>
            <shortName evidence="1">aRqcH</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rqcH</name>
        <name evidence="3" type="ordered locus">SSO0195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably part of the ribosome quality control system (RQC). May mediate the addition of alanine residues (Ala tailing) to incompletely synthesized nascent chains from stalled ribosomes, leading to their degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with stalled 50S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NEMF family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90160">
        <property type="entry name" value="F90160"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922912.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980T2"/>
    <dbReference type="SMR" id="Q980T2"/>
    <dbReference type="FunCoup" id="Q980T2">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0195"/>
    <dbReference type="PaxDb" id="273057-SSO0195"/>
    <dbReference type="EnsemblBacteria" id="AAK40541">
        <property type="protein sequence ID" value="AAK40541"/>
        <property type="gene ID" value="SSO0195"/>
    </dbReference>
    <dbReference type="GeneID" id="1455351"/>
    <dbReference type="GeneID" id="27426490"/>
    <dbReference type="KEGG" id="sso:SSO0195"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.195"/>
    <dbReference type="eggNOG" id="arCOG01695">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003612_2_1_2"/>
    <dbReference type="InParanoid" id="Q980T2"/>
    <dbReference type="PhylomeDB" id="Q980T2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.310.10">
        <property type="entry name" value="ibrinogen binding protein from staphylococcus aureus domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00844_A">
        <property type="entry name" value="RqcH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051608">
        <property type="entry name" value="RQC_Subunit_NEMF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043681">
        <property type="entry name" value="RqcH_archaeal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041120">
        <property type="entry name" value="RqcH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05833">
        <property type="entry name" value="NFACT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="2">
        <location>
            <begin position="401"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="351"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="609" mass="69902" checksum="78F94D15F4A5631F" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Z0</accession>
    <name>Q980Z0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Iron (III) ABC transporter, periplasmic-binding protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90153">
        <property type="entry name" value="C90153"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988954.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Z0"/>
    <dbReference type="STRING" id="273057.SSO0128"/>
    <dbReference type="PaxDb" id="273057-SSO0128"/>
    <dbReference type="DNASU" id="1453309"/>
    <dbReference type="EnsemblBacteria" id="AAK40482">
        <property type="protein sequence ID" value="AAK40482"/>
        <property type="gene ID" value="SSO0128"/>
    </dbReference>
    <dbReference type="GeneID" id="1453309"/>
    <dbReference type="KEGG" id="sso:SSO0128"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.124"/>
    <dbReference type="eggNOG" id="arCOG04233">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_2_8_2"/>
    <dbReference type="InParanoid" id="Q980Z0"/>
    <dbReference type="PhylomeDB" id="Q980Z0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01143">
        <property type="entry name" value="YvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050902">
        <property type="entry name" value="ABC_Transporter_SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535:SF34">
        <property type="entry name" value="MOLYBDATE-BINDING PROTEIN MOLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="22"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="273" mass="31185" checksum="194AD965E69FBBD6" modified="2001-10-01" version="1">MSVRVYNPLQDDYIKIQRPITKIVSLDPASTEIIFLLGLGEKVKATDAFSYRPEDARRTLKIGSYTHVNVDILKQINPDIIFTTAGAQKELTRKLIGLGFNVYPLPVPTNIASILNNVLLIGNVLGVYEKARELYYSLYNVIDGLRARCYKKKIKVYIELDLGGPITVGFPTHISDGISLLGSTNIFDDISEAYFTPKDEEILKRDPDIIIYEPKRLTDYEKERFYSILRKRDLFQLLNKRLIFTKGDYLAHMGPSFITDVLIWLKSVLFSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Z3</accession>
    <name>Q980Z3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90152">
        <property type="entry name" value="H90152"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988949.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Z3"/>
    <dbReference type="SMR" id="Q980Z3"/>
    <dbReference type="STRING" id="273057.SSO0124"/>
    <dbReference type="PaxDb" id="273057-SSO0124"/>
    <dbReference type="EnsemblBacteria" id="AAK40479">
        <property type="protein sequence ID" value="AAK40479"/>
        <property type="gene ID" value="SSO0124"/>
    </dbReference>
    <dbReference type="GeneID" id="1453306"/>
    <dbReference type="KEGG" id="sso:SSO0124"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.120"/>
    <dbReference type="eggNOG" id="arCOG05348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_926288_0_0_2"/>
    <dbReference type="InParanoid" id="Q980Z3"/>
    <dbReference type="PhylomeDB" id="Q980Z3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052541">
        <property type="entry name" value="SQRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755:SF1">
        <property type="entry name" value="FAD-DEPENDENT PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="5"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="300" mass="34536" checksum="DCB690676FD2C285" modified="2001-10-01" version="1">MEAKKILIVGAGYAGLNAYYELGKHLDKTLIADKAQFIFYTAYLQKLIFNKSIQYATNIKPTIINKVKEIDLERKIVKIENGTEIQGHKLILALGCKRESQLDIIRKIIEKDRVSISVENYLDEYLGIQLAFYLRKLNKEVSYYGPVLKWLGEKVSSKVLEFLEKNGIRLSEKSDDIIPACEPNEVIGDFLPVNDKLEYKNGVFVIGDMIKNYPKLGELAMREGVYVGRLLSKKINESFRPIFINIIDTGKGEAIHIRSNILWNGNFESVKVSKIRVIMKRFIERYYIIRKGKMGVLYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q981A6</accession>
    <name>Q981A6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1234</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90144">
        <property type="entry name" value="A90144"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981A6"/>
    <dbReference type="PaxDb" id="273057-SSO0043"/>
    <dbReference type="EnsemblBacteria" id="AAK40408">
        <property type="protein sequence ID" value="AAK40408"/>
        <property type="gene ID" value="SSO0043"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0043"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075479_2_1_2"/>
    <dbReference type="InParanoid" id="Q981A6"/>
    <dbReference type="PhylomeDB" id="Q981A6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="110" mass="12671" checksum="5ADAC3A63B0C81F3" modified="2001-10-01" version="1">MGVEGSTMARTLRHIPNLMYYPLPPIEDMPWREKWLTEIKPVLDAMDLERVLGEGALVYLKLLIVMVLYSCSYRDAVKMVNVNVVVAWFVGRKVGKSTTVSRNLLYFIIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q981D5</accession>
    <name>Q981D5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO5023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90140">
        <property type="entry name" value="B90140"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981D5"/>
    <dbReference type="STRING" id="273057.SSO5023"/>
    <dbReference type="PaxDb" id="273057-SSO5023"/>
    <dbReference type="EnsemblBacteria" id="AAK40377">
        <property type="protein sequence ID" value="AAK40377"/>
        <property type="gene ID" value="SSO5023"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO5023"/>
    <dbReference type="HOGENOM" id="CLU_3094292_0_0_2"/>
    <dbReference type="InParanoid" id="Q981D5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="51" mass="5929" checksum="C624E7AD31FD9E86" modified="2001-10-01" version="1">MIRAFLLLTLFFRPIYSELALVLSLILILLILLLSSLSLLIKILRLLKNIY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-08-29" modified="2026-01-28" version="149" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UWX9</accession>
    <name>ILVC_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ketol-acid reductoisomerase (NADP(+))</fullName>
            <shortName evidence="1">KARI</shortName>
            <ecNumber evidence="1">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acetohydroxy-acid isomeroreductase</fullName>
            <shortName evidence="1">AHIR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ketol-acid reductoisomerase type 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ketol-acid reductoisomerase type I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ilvC</name>
        <name type="synonym">ilvC-1</name>
        <name type="synonym">ilvC1</name>
        <name type="ordered locus">SSO0576</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
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            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="PIR" id="C90204">
        <property type="entry name" value="C90204"/>
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    <dbReference type="RefSeq" id="WP_009991091.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="AlphaFoldDB" id="Q9UWX9"/>
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        <property type="interactions" value="205"/>
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    <dbReference type="STRING" id="273057.SSO0576"/>
    <dbReference type="PaxDb" id="273057-SSO0576"/>
    <dbReference type="EnsemblBacteria" id="AAK40890">
        <property type="protein sequence ID" value="AAK40890"/>
        <property type="gene ID" value="SSO0576"/>
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    <dbReference type="GeneID" id="1454857"/>
    <dbReference type="GeneID" id="44129579"/>
    <dbReference type="KEGG" id="sso:SSO0576"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.584"/>
    <dbReference type="eggNOG" id="arCOG04465">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_033821_0_1_2"/>
    <dbReference type="InParanoid" id="Q9UWX9"/>
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        <property type="reaction ID" value="UER00056"/>
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    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
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        <property type="entry name" value="KARI"/>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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        <property type="entry name" value="PRK05479.1"/>
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    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="KARI_C"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="KARI_N"/>
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    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000116">
        <property type="entry name" value="IlvC_gammaproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151400" description="Ketol-acid reductoisomerase (NADP(+))">
        <location>
            <begin position="1"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="2">
        <location>
            <begin position="5"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="3">
        <location>
            <begin position="186"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="28"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
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        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
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    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="86"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
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        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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            <label>2</label>
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        <location>
            <position position="198"/>
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        <ligand>
            <name>Mg(2+)</name>
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            <label>1</label>
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            <label>2</label>
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            <label>2</label>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="255"/>
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        <ligand>
            <name>substrate</name>
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    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01197"/>
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    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
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    <sequence length="335" mass="36811" checksum="3246B80649CA0516" modified="2000-05-01" version="1">MKCTSKIYTDNDANLDLIKGKRIAVLGYGSQGRAWAQNLRDSGLNVVVGLEREGKSWELAKSDGITPLHTKDAVKDADIIIFLVPDMVQRTLWLESVQPYMKKGADLVFAHGFNIHYKLIDPPKDSDVYMIAPKGPGPTVREYYKAGGGVPALVAVHQDVSGTALHKALAIAKGIGATRAGVIPTTFKEETETDLFGEQVILVGGIMELMRAAFETLVEEGYQPEVAYFETINELKMLVDLVYEKGISGMLKAVSDTAKYGGMTVGKFVIDESVRKRMKEALQRIKSGKFAEEWVEEYGRGMPTVVNGLSNVQNSLEEKIGNQLRDLVQKGKPKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX94</accession>
    <name>RS4E_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein eS4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">30S ribosomal protein S4e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 4" type="primary">rps4e</name>
        <name type="ordered locus">SSO0705</name>
        <name type="ORF">C10_025</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="3">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90218">
        <property type="entry name" value="H90218"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991269.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="E=1-239"/>
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    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="E=1-239"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="E=1-239"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="E=1-239"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="E=1-239"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="E=1-239"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="E=1-239"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="E=1-239"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q9UX94"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q9UX94"/>
    <dbReference type="FunCoup" id="Q9UX94">
        <property type="interactions" value="180"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0705"/>
    <dbReference type="PaxDb" id="273057-SSO0705"/>
    <dbReference type="EnsemblBacteria" id="AAK41007">
        <property type="protein sequence ID" value="AAK41007"/>
        <property type="gene ID" value="SSO0705"/>
    </dbReference>
    <dbReference type="GeneID" id="1454974"/>
    <dbReference type="KEGG" id="sso:SSO0705"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.706"/>
    <dbReference type="eggNOG" id="arCOG04093">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060400_0_0_2"/>
    <dbReference type="InParanoid" id="Q9UX94"/>
    <dbReference type="PhylomeDB" id="Q9UX94"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_eS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_eS4_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_eS4_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="Ribosomal_eS4_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_eS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003312">
        <property type="entry name" value="PRK04313.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000130863" description="Small ribosomal subunit protein eS4">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding" evidence="1">
        <location>
            <begin position="37"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000312" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="CAB57598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="239" mass="27519" checksum="1A72881FA85860F5" modified="2000-05-01" version="1">MAHITRFEAPWFLMISKKQYKWTVRPNAGPHSIEKSIPLAVVIRDYLKLAGTIREAKHIIFDGKVLVDGKVRKDYKYPVGLMDIVSIPSADLYFRVLPDNVRFMRFSKISADEARYKYVRIINKTTIKEGRIQLNLEDGRNILVDKETAKNFKTLMTLKIELPSQQILDSFTISERSYAIFVGGRNVGIHGIVKNINLSKFKSRKYSVITLESRDGNTYQTNIMNVMSIGREKSDLRVD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-20" modified="2025-06-18" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXB1</accession>
    <name>SYI_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Isoleucine--tRNA ligase</fullName>
            <ecNumber>6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Isoleucyl-tRNA synthetase</fullName>
            <shortName>IleRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ileS</name>
        <name type="ordered locus">SSO0722</name>
        <name type="ORF">C10_008</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90220">
        <property type="entry name" value="E90220"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXB1"/>
    <dbReference type="SMR" id="Q9UXB1"/>
    <dbReference type="FunCoup" id="Q9UXB1">
        <property type="interactions" value="302"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0722"/>
    <dbReference type="PaxDb" id="273057-SSO0722"/>
    <dbReference type="EnsemblBacteria" id="AAK41020">
        <property type="protein sequence ID" value="AAK41020"/>
        <property type="gene ID" value="SSO0722"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0722"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.720"/>
    <dbReference type="eggNOG" id="arCOG00807">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_1_1_2"/>
    <dbReference type="InParanoid" id="Q9UXB1"/>
    <dbReference type="PhylomeDB" id="Q9UXB1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07961">
        <property type="entry name" value="Anticodon_Ia_Ile_ABEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00818">
        <property type="entry name" value="IleRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000083">
        <property type="entry name" value="Isoleucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000325">
        <property type="entry name" value="Isoleucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000041">
        <property type="entry name" value="Isoleucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033709">
        <property type="entry name" value="Anticodon_Ile_ABEc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050081">
        <property type="entry name" value="Ile-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19302">
        <property type="entry name" value="DUF5915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000098593" description="Isoleucine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="534"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="537"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="986" mass="114714" checksum="0177A3C31280F5A1" modified="2000-05-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="144" xmlns="https://uniprot.org/uniprot">
    <accession>O06457</accession>
    <name>PURK_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N5-carboxyaminoimidazole ribonucleotide synthase</fullName>
            <shortName evidence="1">N5-CAIR synthase</shortName>
            <ecNumber evidence="1">6.3.4.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-(carboxyamino)imidazole ribonucleotide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purK</name>
        <name type="ordered locus">SSO1065</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="FEMS Microbiol. Lett." volume="154" first="173" last="180">
            <title>Identification and sequence analysis of Sulfolobus solfataricus purE and purK genes.</title>
            <authorList>
                <person name="Soerensen I.S."/>
                <person name="Dandanell G."/>
            </authorList>
            <dbReference type="PubMed" id="9311113"/>
            <dbReference type="DOI" id="10.1016/S0378-1097(97)00320-0"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + hydrogencarbonate + ATP = 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:19317"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PurK/PurT family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.18" evidence="1"/>
    <dbReference type="EMBL" id="Y13143">
        <property type="protein sequence ID" value="CAA73603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90259">
        <property type="entry name" value="A90259"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989884.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O06457"/>
    <dbReference type="SMR" id="O06457"/>
    <dbReference type="FunCoup" id="O06457">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1065"/>
    <dbReference type="PaxDb" id="273057-SSO1065"/>
    <dbReference type="EnsemblBacteria" id="AAK41328">
        <property type="protein sequence ID" value="AAK41328"/>
        <property type="gene ID" value="SSO1065"/>
    </dbReference>
    <dbReference type="GeneID" id="1454107"/>
    <dbReference type="GeneID" id="44129999"/>
    <dbReference type="KEGG" id="sso:SSO1065"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1062"/>
    <dbReference type="eggNOG" id="arCOG01597">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011534_0_0_2"/>
    <dbReference type="InParanoid" id="O06457"/>
    <dbReference type="PhylomeDB" id="O06457"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00942"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034028">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004638">
        <property type="term" value="F:phosphoribosylaminoimidazole carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054350">
        <property type="entry name" value="PurT/PurK_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004679">
        <property type="entry name" value="PRK06019.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01161">
        <property type="entry name" value="purK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609:SF5">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609">
        <property type="entry name" value="PURINE BIOSYNTHESIS PROTEIN 6/7, PUR6/7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22660">
        <property type="entry name" value="RS_preATP-grasp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000075020" description="N5-carboxyaminoimidazole ribonucleotide synthase">
        <location>
            <begin position="1"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="1">
        <location>
            <begin position="106"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="148"/>
            <end position="154"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="177"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="256"/>
            <end position="257"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01928"/>
        </source>
    </evidence>
    <sequence length="365" mass="42360" checksum="A9EE75D029EE199C" modified="1997-07-01" version="1">MFSVLDWKPKIGILGGGQLGWMIVLEGRKYPFTFYVLENDKNAPACRIADRCFSPQDYKEFVDSSDVITFEFEHVYEKALEYAEYSGKLLPRLNSVELKRERYKEKLFYRQHNLPTPRFYVAEDGEEALKILREEFNNVGVIKESKGGYDGKGQYFIFNDVEKYQFLREKKEKMVVEEYVKFDFEASIIIARDKRGVFISYPPTYNYNEKGILVYNYGPYNNQNIVEIARRLSEELDYVGIMGVEVFVVNGKVLINEFAPRVHNTGHYTLDGALISQFEQHLRAIIGMELGPSTILSPSGMVNILGTDKIPVEVLKYGKVYWYSKSEVRKRRKMGHVNVVGNNLEEVKQKIDKIMQLIYTNGLDL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="153" xmlns="https://uniprot.org/uniprot">
    <accession>P80857</accession>
    <name>SODF_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Superoxide dismutase [Fe]</fullName>
            <ecNumber>1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">sod</name>
        <name type="ordered locus">SSO0316</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1999" name="J. Biochem." volume="125" first="186" last="193">
            <title>An azide-insensitive superoxide dismutase from a hyperthermophilic archaeon, Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Yamano S."/>
                <person name="Maruyama T."/>
            </authorList>
            <dbReference type="PubMed" id="9880816"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Eur. J. Biochem." volume="268" first="1794" last="1801">
            <title>Phenylmethanesulfonyl fluoride inactivates an archaeal superoxide dismutase by chemical modification of a specific tyrosine residue: cloning, sequencing and expression of the gene coding for Sulfolobus solfataricus superoxide dismutase.</title>
            <authorList>
                <person name="De Vendittis E."/>
                <person name="Ursby T."/>
                <person name="Rullo R."/>
                <person name="Gogliettino M.A."/>
                <person name="Masullo M."/>
                <person name="Bocchini V."/>
            </authorList>
            <dbReference type="PubMed" id="11248699"/>
            <dbReference type="DOI" id="10.1046/j.1432-1327.2001.02052.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="1997" name="Biochim. Biophys. Acta" volume="1343" first="23" last="30">
            <title>Iron superoxide dismutase from the archaeon Sulfolobus solfataricus: average hydrophobicity and amino acid weight are involved in the adaptation of proteins to extreme environments.</title>
            <authorList>
                <person name="Dello Russo A."/>
                <person name="Rullo R."/>
                <person name="Nitti G."/>
                <person name="Masullo M."/>
                <person name="Bocchini V."/>
            </authorList>
            <dbReference type="PubMed" id="9428655"/>
            <dbReference type="DOI" id="10.1016/S0167-4838(97)00105-2"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-211</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="1999" name="J. Mol. Biol." volume="286" first="189" last="205">
            <title>Iron superoxide dismutase from the archaeon Sulfolobus solfataricus: analysis of structure and thermostability.</title>
            <authorList>
                <person name="Ursby T."/>
                <person name="Adinolfi B.S."/>
                <person name="Al-Karadaghi S."/>
                <person name="de Vendittis E."/>
                <person name="Bocchini V."/>
            </authorList>
            <dbReference type="PubMed" id="9931259"/>
            <dbReference type="DOI" id="10.1006/jmbi.1998.2471"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH IRON IONS</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text>Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text>Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1"/>
    <dbReference type="EMBL" id="AB012620">
        <property type="protein sequence ID" value="BAA75509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y15326">
        <property type="protein sequence ID" value="CAA75583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
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</entry>
<entry dataset="Swiss-Prot" created="2001-10-18" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>P95962</accession>
    <name>Y099_SACS2</name>
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        <recommendedName>
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            <taxon>Thermoproteota</taxon>
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            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the UPF0173 family.</text>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
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        </source>
    </evidence>
    <sequence length="227" mass="25108" checksum="0CF15D903E30364E" modified="1997-05-01" version="1">MAQLRWLGHAATLLTFGNKNVIIDPMIKDNPLSPVKLDYFKNNLDIIIVTHDHYDHLGDTVELLRMNPKAKLFATYDLEAHLAETYKISEESIIPANVGGFVEVDGIKLALTKAVHSSTHSDPTGAIVSAEGITVYHAGDTGLFEDMKLIGEVFKPDYALLPIGGRFTMDPYQASISVELIKPKKGAIPIHYNTWDLIKVDVNDFVKLVKNKGYNPIVLQPGQTITL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2025-06-18" version="149" xmlns="https://uniprot.org/uniprot">
    <accession>P95970</accession>
    <name>SYK_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Lysine--tRNA ligase</fullName>
            <ecNumber>6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Lysyl-tRNA synthetase</fullName>
            <shortName>LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">lysS</name>
        <name type="ordered locus">SSO0090</name>
        <name type="ORF">C04_032</name>
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    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 3 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6"/>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75398">
        <property type="entry name" value="S75398"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95970"/>
    <dbReference type="SMR" id="P95970"/>
    <dbReference type="FunCoup" id="P95970">
        <property type="interactions" value="349"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0090"/>
    <dbReference type="PaxDb" id="273057-SSO0090"/>
    <dbReference type="EnsemblBacteria" id="AAK40448">
        <property type="protein sequence ID" value="AAK40448"/>
        <property type="gene ID" value="SSO0090"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0090"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.87"/>
    <dbReference type="eggNOG" id="arCOG00408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008255_6_0_2"/>
    <dbReference type="InParanoid" id="P95970"/>
    <dbReference type="PhylomeDB" id="P95970"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00775">
        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000151">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00252">
        <property type="entry name" value="Lys_tRNA_synth_class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001756">
        <property type="entry name" value="PRK00484.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918:SF15">
        <property type="entry name" value="LYSINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918">
        <property type="entry name" value="LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152714" description="Lysine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="494" mass="57801" checksum="AF19750B74CE2443" modified="1997-05-01" version="1">MLMNWDERRVKIVEELRKNGIEPYPHKYEVTHLIKDIKLIAKSQGNKSHEPFMFNISTAGRVANIRRHGKASFVDIFDEGEKLQLYMRVDELKEKYDKFFIYVGRGDIIGVKGDLFYTMKGELSLLVKDYQLLSKALIEPPDWSKLSPEFRYAHRYVDFLYNDNARKAMEIRYTIIREIREFLYSKGFIEVETPIVQPVYGGALAKPFKTHVNYLNEDWYLRIALELYLKRYIIGGFNKVFEIGKVFRNEDIDVTHNPEFTLLELYWAYADYNDIMNLTEDLLKSVVKKVTNSTKIVYGKYEIDFEGPFKRISMYDSLSEILGKDVESMSDNELKELMKKYNLTPRGNQYVRGLMIEKLFDKLVTPTLTNPTFITDYPIETTPLCKPHRNKPGLVERFEMFIAGMEVANAYTELNDPILQDKLFREEQEMFRRGDEEAHPYDKDFVRALSYGMPPTGGLGIGIDRIVMLVTNNYSIKEVIPFPMISSKVILEDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="137" xmlns="https://uniprot.org/uniprot">
    <accession>P95986</accession>
    <name>RS13_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Small ribosomal subunit protein uS13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">30S ribosomal protein S13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps13</name>
        <name evidence="6" type="synonym">rps13AB</name>
        <name type="ordered locus">SSO0074</name>
        <name type="ORF">C04_048</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7 8 9" key="3">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3">Located at the top of the head of the 30S subunit (PubMed:39753558), it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement.</text>
    </comment>
    <comment type="function">
        <text evidence="3">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). uS9, uS13 and uS19 stabilize the codon:anticodon duplex (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 3">Part of the 30S ribosomal subunit (PubMed:39753558). Forms a loose heterodimer with protein S19 (PubMed:39753558). Forms two bridges to the 50S subunit in the 70S ribosome. Interacts with eS25 (PubMed:39753558).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75414">
        <property type="entry name" value="S75414"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988886.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="O=1-165"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="O=1-165"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="O=1-165"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="O=1-165"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="O=1-165"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="O=1-165"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="O=1-165"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="O=1-165"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="P95986"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="P95986"/>
    <dbReference type="FunCoup" id="P95986">
        <property type="interactions" value="272"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0074"/>
    <dbReference type="PaxDb" id="273057-SSO0074"/>
    <dbReference type="EnsemblBacteria" id="AAK40436">
        <property type="protein sequence ID" value="AAK40436"/>
        <property type="gene ID" value="SSO0074"/>
    </dbReference>
    <dbReference type="GeneID" id="1455327"/>
    <dbReference type="KEGG" id="sso:SSO0074"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.75"/>
    <dbReference type="eggNOG" id="arCOG01722">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103849_0_0_2"/>
    <dbReference type="InParanoid" id="P95986"/>
    <dbReference type="PhylomeDB" id="P95986"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.50:FF:000001">
        <property type="entry name" value="30S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.910.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="entry name" value="30s ribosomal protein s13, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_uS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="Rbsml_uS13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_uS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019977">
        <property type="entry name" value="Ribosomal_uS13_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_uS13_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003140">
        <property type="entry name" value="PRK04053.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03629">
        <property type="entry name" value="uS13_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000132191" description="Small ribosomal subunit protein uS13">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="139"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="153"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="165" mass="18629" checksum="8F4DAAEBE8736751" modified="1997-05-01" version="1">MSQQFKYVVRIFGQDVDGTMKLPYALAMVKGIGYNTAKAIIRKLGMDPNARLGELSDAEVKKVESVISDHTIKGLPSWLYNRRKDYESGLDLHLVTSDLIFYVRNDIEREKKSRSWRGVRHSLGLKVRGQRTRTTGRTGMTIGVARKKAAQPQSQQSSSQQQKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX47</accession>
    <name>Q7LX47_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase small domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74065">
        <property type="entry name" value="S74065"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX47"/>
    <dbReference type="FunCoup" id="Q7LX47">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2158"/>
    <dbReference type="PaxDb" id="273057-SSO2158"/>
    <dbReference type="EnsemblBacteria" id="AAK42334">
        <property type="protein sequence ID" value="AAK42334"/>
        <property type="gene ID" value="SSO2158"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2158"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2251"/>
    <dbReference type="eggNOG" id="arCOG00910">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2021600_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX47"/>
    <dbReference type="PhylomeDB" id="Q7LX47"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051720">
        <property type="entry name" value="rRNA_MeTrfase/Polyamine_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290:SF0">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="15043" checksum="FD241A0034D10360" modified="2011-05-31" version="1">MEIDMESLDTVKNMKSELDLDIELINADATQFYSKFDTVIQNPPFGVVNRGIDIKFLQTAFSISDVVYSIHKSNERSREIIITMAKDYGFSTEILSERFRLKPYYPWHMRRVHEFLVDIYFFSKISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX87</accession>
    <name>Q7LX87_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PaREP1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74021">
        <property type="entry name" value="S74021"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990136.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX87"/>
    <dbReference type="SMR" id="Q7LX87"/>
    <dbReference type="PaxDb" id="273057-SSO2109"/>
    <dbReference type="DNASU" id="1453615"/>
    <dbReference type="EnsemblBacteria" id="AAK42288">
        <property type="protein sequence ID" value="AAK42288"/>
        <property type="gene ID" value="SSO2109"/>
    </dbReference>
    <dbReference type="GeneID" id="1453615"/>
    <dbReference type="KEGG" id="sso:SSO2109"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2195"/>
    <dbReference type="eggNOG" id="arCOG03721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115256_2_0_2"/>
    <dbReference type="InParanoid" id="Q7LX87"/>
    <dbReference type="PhylomeDB" id="Q7LX87"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="13992" checksum="F1B3E0E927EA98BD" modified="2011-05-31" version="1">MSISTSAEVYYEEAEEFLSKGDLVQACEKYYKAAEEAIKLLVIENNLKEITNNVKNKGRWKSENLFKASKLLRSNNTEIPILWKSAWTLHVEGFHELSLNEKEVKKLKEDVRKLVIFAVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXI4</accession>
    <name>Q7LXI4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1177 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0789</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90228">
        <property type="entry name" value="H90228"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923082.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXI4"/>
    <dbReference type="STRING" id="273057.SSO0789"/>
    <dbReference type="PaxDb" id="273057-SSO0789"/>
    <dbReference type="EnsemblBacteria" id="AAK41087">
        <property type="protein sequence ID" value="AAK41087"/>
        <property type="gene ID" value="SSO0789"/>
    </dbReference>
    <dbReference type="GeneID" id="1455054"/>
    <dbReference type="KEGG" id="sso:SSO0789"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.794"/>
    <dbReference type="eggNOG" id="arCOG05881">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077081_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXI4"/>
    <dbReference type="PhylomeDB" id="Q7LXI4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009561">
        <property type="entry name" value="DUF1177"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06675">
        <property type="entry name" value="DUF1177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="32986" checksum="2BD7352995AC915C" modified="2011-05-31" version="1">MMLKSLIDAIEILESKDPLNLLRSKLKGVEYEEKKIGEVTFVKVYYGSGGREKVEILGRLGAIQMQNASKGLVSDADGAIVTLAVLLELLNLKEKGIALDLDVSFVTNISLNAKLIPHQPFNFMVPLIGLDEALKEEVDPKAGLILSIDSTKGNRLAKFDDFALTHVIKDGYILKLKDEVIDIYNRVTGHEVYLVSLTTGDLTPLDFNVYHISTLISPWLYTDSPIIGIATVSKQMIPGYVTGVQDIEMLEHASRFCLEMLKYVEGGGKVYDENELKELKEKLGESNLQRVKKWKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXI5</accession>
    <name>Q7LXI5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0788</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90228">
        <property type="entry name" value="G90228"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923081.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXI5"/>
    <dbReference type="STRING" id="273057.SSO0788"/>
    <dbReference type="PaxDb" id="273057-SSO0788"/>
    <dbReference type="EnsemblBacteria" id="AAK41086">
        <property type="protein sequence ID" value="AAK41086"/>
        <property type="gene ID" value="SSO0788"/>
    </dbReference>
    <dbReference type="GeneID" id="1455053"/>
    <dbReference type="KEGG" id="sso:SSO0788"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.793"/>
    <dbReference type="eggNOG" id="arCOG00340">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_9_2_2"/>
    <dbReference type="InParanoid" id="Q7LXI5"/>
    <dbReference type="PhylomeDB" id="Q7LXI5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF118">
        <property type="entry name" value="ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE (PRECURSOR)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="352" mass="40113" checksum="7D926E220D8D9792" modified="2011-05-31" version="1">MKLCDEYRIPLVPRGAGTSTIGQVLPIYSSIILDMNDMKEVIEIDDKWVKVTPSVKILRTLDYLRRRGKELRVYPSSFYIATLGGYIAGGDVGIGSFQYGYHFDNNGINSVRVLGVTGKYTLNGKDTLAVAQAAGTTGVITEAELAIIDYEDWKDQLIRLNDLEEVVKILKKLGEFRSKIRRITVEDYEALSLVGKDRLDKVGKWNVIVASTLSFGEEVNLRFLDELAFAAIYVTMSKLTKFKDYFYEVRLLSLDSFLRVVKQVKEALNSNVLIHGDVMTLRGEIIIYTVFISDKSNFEIIDSIMLKEGIPFEIHSIEVNDRVDEEYRLELMKKYKRIMDPHNILNPGKLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXI9</accession>
    <name>Q7LXI9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1286 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90228">
        <property type="entry name" value="C90228"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXI9"/>
    <dbReference type="PaxDb" id="273057-SSO0784"/>
    <dbReference type="DNASU" id="1455049"/>
    <dbReference type="EnsemblBacteria" id="AAK41082">
        <property type="protein sequence ID" value="AAK41082"/>
        <property type="gene ID" value="SSO0784"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0784"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.787"/>
    <dbReference type="eggNOG" id="arCOG05320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117041_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXI9"/>
    <dbReference type="PhylomeDB" id="Q7LXI9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009705">
        <property type="entry name" value="DUF1286"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06939">
        <property type="entry name" value="DUF1286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22282" checksum="0490AB76D64FB31B" modified="2011-05-31" version="1">MRKSVSRASSIITGLLVMSIGLNSTEYLSIYKLILFNKKTCMKLKTHFVFSIGLLTLASSFFGFSLIISAIISVLGNTLIDRLGHKEISTRYGKIPVRTPLTHTLPRSVFWGLISSMPVIIVLGLAYGMLNTKILTALIDGILVGPSHMLLDVFTENGIYVKRNGKWKRVALAHFSYDSSLINGLAIVLGIIMIFAAMELHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXN6</accession>
    <name>Q7LXN6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0674</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99215">
        <property type="entry name" value="A99215"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991219.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXN6"/>
    <dbReference type="SMR" id="Q7LXN6"/>
    <dbReference type="STRING" id="273057.SSO0674"/>
    <dbReference type="PaxDb" id="273057-SSO0674"/>
    <dbReference type="EnsemblBacteria" id="AAK40976">
        <property type="protein sequence ID" value="AAK40976"/>
        <property type="gene ID" value="SSO0674"/>
    </dbReference>
    <dbReference type="GeneID" id="1454943"/>
    <dbReference type="KEGG" id="sso:SSO0674"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.674"/>
    <dbReference type="eggNOG" id="arCOG00600">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1243045_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXN6"/>
    <dbReference type="PhylomeDB" id="Q7LXN6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="124"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="240" mass="27007" checksum="4C303E905D056935" modified="2011-05-31" version="1">MSVQINGAEIISLDPNAYVTDALFFMRRNNVRRLVVSNSSNIIGVFTIENAIRQILENRLEVRLNELKLRRPVYVEDNYIKSIVGTMIGESSDFVLYRGKYIITEKDVVRSFNWSSVKGSVEGISKDAIVVQPFTKISTCIETMLKNNIRHLPIVNGIALGIVSARDIAYSYDSVTGNTIVEKIMNVNLVTADDNSELSYAINLMIGRNVGSLIVKTRVKNKVKILTNRDLIKLIFSYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXP8</accession>
    <name>Q7LXP8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homoserine dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">thrA/hom</name>
        <name evidence="14" type="ordered locus">SSO0657</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C99213">
        <property type="entry name" value="C99213"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991197.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXP8"/>
    <dbReference type="FunCoup" id="Q7LXP8">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0657"/>
    <dbReference type="PaxDb" id="273057-SSO0657"/>
    <dbReference type="EnsemblBacteria" id="AAK40962">
        <property type="protein sequence ID" value="AAK40962"/>
        <property type="gene ID" value="SSO0657"/>
    </dbReference>
    <dbReference type="GeneID" id="1454929"/>
    <dbReference type="KEGG" id="sso:SSO0657"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.659"/>
    <dbReference type="eggNOG" id="arCOG01351">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_1_2_2"/>
    <dbReference type="InParanoid" id="Q7LXP8"/>
    <dbReference type="PhylomeDB" id="Q7LXP8"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000005">
        <property type="entry name" value="Homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000554">
        <property type="entry name" value="Homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022697">
        <property type="entry name" value="HDH_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004976">
        <property type="entry name" value="PRK06349.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43331">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43331:SF1">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036497">
        <property type="entry name" value="HDH_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Aspartate/homoserine dehydrogenase NAD-binding" evidence="13">
        <location>
            <begin position="7"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Homoserine dehydrogenase catalytic" evidence="12">
        <location>
            <begin position="144"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>L-homoserine</name>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAK40962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="314" mass="34690" checksum="38CF42C51D26AC10" modified="2011-05-31" version="1">MKLLLIGYGNVGKAFRRLLHEKKDNYPILQGVEIAGIITRQGLMVGDKENFSPDKQLTVLEAIDYINPDVIVDMSVPNYKDGEPSVSAYIKALSRKIHVITVNKAPLALKFREIFEVAEKTGAKIGFQGTVMSGTPSINLFRIQPVAEVSRIRGILNGTTNYILTRIYEGLSFNEALKEAKEKGYAEEDPTLDLNGFDAAAKLTILSNFIMNRSLKLGDFKFKGITEITNEEIRRAKSEGKKIKLIAYADNYVVQVSPQIIGPQDPLYYIDGVENALEIQNEIQRIIIRGPGAGPINAAYGALSDLVLLLEGCL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2019-10-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXU6</accession>
    <name>AIPS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Archaetidylinositol phosphate synthase</fullName>
            <shortName evidence="6">AIP synthase</shortName>
            <ecNumber evidence="5">2.7.8.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pgsA</name>
        <name evidence="9" type="ordered locus">SSO0556</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2014" name="Biochem. Biophys. Res. Commun." volume="443" first="86" last="90">
            <title>Ubiquitous distribution of phosphatidylinositol phosphate synthase and archaetidylinositol phosphate synthase in Bacteria and Archaea, which contain inositol phospholipid.</title>
            <authorList>
                <person name="Morii H."/>
                <person name="Ogawa M."/>
                <person name="Fukuda K."/>
                <person name="Taniguchi H."/>
            </authorList>
            <dbReference type="PubMed" id="24269814"/>
            <dbReference type="DOI" id="10.1016/j.bbrc.2013.11.054"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PATHWAY</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the formation of archaetidylinositol phosphate (AIP) from CDP-archaeol (CDP-ArOH or CDP-2,3-bis-(O-phytanyl)-sn-glycerol) and 1L-myo-inositol 1-phosphate (IP or 1D-myo-inositol 3-phosphate). AIP is a precursor of archaetidyl-myo-inositol (AI), an ether-type inositol phospholipid ubiquitously distributed in archaea membranes and essential for glycolipid biosynthesis in archaea.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1D-myo-inositol 3-phosphate = saturated 1-archaetidyl-1D-myo-inositol 3-phosphate + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36823"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:74004"/>
            <dbReference type="ChEBI" id="CHEBI:74006"/>
            <dbReference type="EC" id="2.7.8.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 Mg(2+) or Mn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.39" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
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        <property type="entry name" value="B90202"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="EnsemblBacteria" id="AAK40873">
        <property type="protein sequence ID" value="AAK40873"/>
        <property type="gene ID" value="SSO0556"/>
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    <dbReference type="GeneID" id="44129562"/>
    <dbReference type="KEGG" id="sso:SSO0556"/>
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        <property type="organism ID" value="6163"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:phospholipid biosynthetic process"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
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    </dbReference>
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    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000448365" description="Archaetidylinositol phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
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            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
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            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
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            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="67"/>
        </location>
        <ligand>
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            <label>1</label>
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        <ligand>
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            <label>2</label>
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        <location>
            <position position="70"/>
        </location>
        <ligand>
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        <location>
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        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O27726"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="UniProtKB" id="P9WPG7"/>
        </source>
    </evidence>
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    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="24269814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="6">
        <source>
            <dbReference type="PubMed" id="24269814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <evidence type="ECO:0000305" key="8">
        <source>
            <dbReference type="PubMed" id="24269814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="9">
        <source>
            <dbReference type="EMBL" id="AAK40873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK40873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="195" mass="22095" checksum="D6A1323EC4E8A967" modified="2011-05-31" version="1">MVSMLTRIRRQIKRVIEPLAKTLAQLKVSANFITMLGLIFAIVYYFEIMRSNTTLGIIFLVFSALMDAIDGEVARVSKTVSPLGSFLDSTLDRIEDILYISAFIFLGFSSYLVAIAVGLSLTISYIRAKAESLGLKMEGRGIIERGERIIFVFVILLLYIIVSKQVSLYLFYLFMLLTAITVIQRFYAVYSLLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXU7</accession>
    <name>Q7LXU7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="GeneID" id="1454839"/>
    <dbReference type="KEGG" id="sso:SSO0555"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.565"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2204008_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXU7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="103" mass="11707" checksum="21462045326D2AC6" modified="2011-05-31" version="1">MTAKKLILVTSESHPLHKVFMETLEELSKELNLEKEVKIEDYAFLADYGEKDEFDMPFLPQLLAQTDDGKIIPVLTKMPFDASLKPDKKAGKEEAIKKIKNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXV3</accession>
    <name>Q7LXV3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Coenzyme PQQ synthesis protein E (PqqE-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pqqE-1</name>
        <name evidence="8" type="ordered locus">SSO0546</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90201">
        <property type="entry name" value="A90201"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992824.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXV3"/>
    <dbReference type="SMR" id="Q7LXV3"/>
    <dbReference type="STRING" id="273057.SSO0546"/>
    <dbReference type="PaxDb" id="273057-SSO0546"/>
    <dbReference type="EnsemblBacteria" id="AAK40864">
        <property type="protein sequence ID" value="AAK40864"/>
        <property type="gene ID" value="SSO0546"/>
    </dbReference>
    <dbReference type="GeneID" id="54123227"/>
    <dbReference type="KEGG" id="sso:SSO0546"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.552"/>
    <dbReference type="eggNOG" id="arCOG00931">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_7_2"/>
    <dbReference type="InParanoid" id="Q7LXV3"/>
    <dbReference type="PhylomeDB" id="Q7LXV3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21123">
        <property type="entry name" value="SPASM_MftC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017200">
        <property type="entry name" value="PqqE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF34">
        <property type="entry name" value="TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE COFACTOR MODIFYING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037420">
        <property type="entry name" value="PQQ_syn_pqqE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="3"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK40864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="361" mass="41614" checksum="6090FDDE7104F81D" modified="2011-05-31" version="1">MINRKPFFIELELTYKCSQQCIYCHNPPKDLKFYIADKVPKPRSVIEMNELNLSEWISVINQIKRLDEKYEGIMDVVITGGEPLLRSDLEEILSYVTRQKLRHMLLSSGEPISDDRITKLLESGLEKIRINLTSHKYVFNIQSPIMHIKNEVAKKINIAKKFKDFGVKEVGGNIVLTSYYLDFLEEIAELAYKSNLDWIEIHSVIKVGHGYINQKFIVPDPNDERTIQEKLKRLIEIYGENFIQNYVDTDILYSRIPIPTNWGEVGLVIAPNGDIYPSSEATSIPLSKLGNIKNDSLVEIWFNNLLLNKIRSLSFLREPCKSCEVKKLCRGGFRFNAYITKNDLFAPDPSCQIVKDYIGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXW1</accession>
    <name>Q7LXW1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0537</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99200">
        <property type="entry name" value="A99200"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991038.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXW1"/>
    <dbReference type="PaxDb" id="273057-SSO0537"/>
    <dbReference type="EnsemblBacteria" id="AAK40856">
        <property type="protein sequence ID" value="AAK40856"/>
        <property type="gene ID" value="SSO0537"/>
    </dbReference>
    <dbReference type="GeneID" id="1454823"/>
    <dbReference type="KEGG" id="sso:SSO0537"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.536"/>
    <dbReference type="eggNOG" id="arCOG07218">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1021646_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXW1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="273" mass="30456" checksum="735683932A14E128" modified="2011-05-31" version="1">MAKSIGIGSILIIISIIIIGSVATIFYLENVDVNISVNPIYWRIYSNNYEVIAFAITATNNAPLTQTLNITLLSRYLVPGSNGVLKIIANSTTITLKSHSSSTAIVYLTFSSSTLFPEIEEFKVLVHGLYGYSKQFGDVNTIYTTGLTRNIALLMGINQNQLPTFSLVGSFTPSANFNYSYSIVYYQYNYTSNTSFLFTKFTISPLANQYLQPGKYNVTLSYLDHANSFLINIPRNTPVYFYLFVPMANFNMTVILQGQGVKYQGYFTVSIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXX1</accession>
    <name>Q7LXX1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90197">
        <property type="entry name" value="G90197"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923022.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXX1"/>
    <dbReference type="PaxDb" id="273057-SSO0520"/>
    <dbReference type="EnsemblBacteria" id="AAK40838">
        <property type="protein sequence ID" value="AAK40838"/>
        <property type="gene ID" value="SSO0520"/>
    </dbReference>
    <dbReference type="GeneID" id="1454805"/>
    <dbReference type="KEGG" id="sso:SSO0520"/>
    <dbReference type="eggNOG" id="arCOG07733">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2103615_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXX1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="104" mass="11494" checksum="35889193A0C6D5F3" modified="2011-05-31" version="1">MKKSVLTTLVIALVLLGTSLYLYSVNHPEKTVITIPSPYTYTVSINTTTLTAPIGVPFEGPIIATTFIHTNYTPPPYYASLWLETLAVGIVLLLISILLLRRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LYK1</accession>
    <name>Q7LYK1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74006">
        <property type="entry name" value="S74006"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LYK1"/>
    <dbReference type="STRING" id="273057.SSO2076"/>
    <dbReference type="PaxDb" id="273057-SSO2076"/>
    <dbReference type="EnsemblBacteria" id="AAK42257">
        <property type="protein sequence ID" value="AAK42257"/>
        <property type="gene ID" value="SSO2076"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2076"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2153"/>
    <dbReference type="eggNOG" id="arCOG04531">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2056162_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LYK1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="119" mass="12921" checksum="A880953F739586F4" modified="2011-05-31" version="1">MRRVGSKNCSRGGENRKVLLEALGKNNYKNSVKVYIIESIPAILIVSLMLGKYSSIINFALTLLSLSPVVIAIPAVLVGILISNKRIMGKYAYTKTRLLAYFLTVAMIVAGGLVGILHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TY7</accession>
    <name>Q97TY7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Aspartate aminotransferase (AspB-4)</fullName>
            <ecNumber evidence="8">2.6.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">aspB-4</name>
        <name evidence="8" type="ordered locus">SSO3245</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90510">
        <property type="entry name" value="B90510"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989665.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TY7"/>
    <dbReference type="FunCoup" id="Q97TY7">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3245"/>
    <dbReference type="PaxDb" id="273057-SSO3245"/>
    <dbReference type="EnsemblBacteria" id="AAK43337">
        <property type="protein sequence ID" value="AAK43337"/>
        <property type="gene ID" value="SSO3245"/>
    </dbReference>
    <dbReference type="GeneID" id="1453256"/>
    <dbReference type="KEGG" id="sso:SSO3245"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3344"/>
    <dbReference type="eggNOG" id="arCOG00492">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_6_2"/>
    <dbReference type="InParanoid" id="Q97TY7"/>
    <dbReference type="PhylomeDB" id="Q97TY7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000053">
        <property type="entry name" value="Aminotransferase, class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF19">
        <property type="entry name" value="KYNURENINE_ALPHA-AMINOADIPATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="25"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK43337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="380" mass="42795" checksum="2C85D67EEC938170" modified="2001-10-01" version="1">MVSRIGKEIEISPVELGSQIAKRVKINMASGTPDPRKMPIKEIKDAYSEVLNELGPKVLFYPGAGGQEELIREIEDKFLSVLNLTKRKDEKIVVTSGAQHAMELLGKYFLENDVIAVENPTFIETFNALKLRSSSVIPISLDPSGIDVNELETLLKVVKIRLLYVIPNCHNPAGVNMSECRRKRLIELAEKYDFYIIEDDPYRPIAGETPQPIKNYDNNGRVIYVSSFSKIIAPGLRVGFLIAKEDIAEKISLMEQLDFSTSTINQYVVARLLRKGVVTERSKELYEHYKSKMKILVDSLVDKGLDKFNEPKCGFFLLLDVGKDSWNVFHKAVEKGLSFVPAKPFFLRGGDTMARLSVSVATKEEIIEGVKILNEVIRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U24</accession>
    <name>Q97U24_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Oxidoreductase molybdopterin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90505">
        <property type="entry name" value="C90505"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991681.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U24"/>
    <dbReference type="FunCoup" id="Q97U24">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3201"/>
    <dbReference type="PaxDb" id="273057-SSO3201"/>
    <dbReference type="EnsemblBacteria" id="AAK43298">
        <property type="protein sequence ID" value="AAK43298"/>
        <property type="gene ID" value="SSO3201"/>
    </dbReference>
    <dbReference type="GeneID" id="1453217"/>
    <dbReference type="KEGG" id="sso:SSO3201"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3303"/>
    <dbReference type="eggNOG" id="arCOG00264">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094953_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U24"/>
    <dbReference type="PhylomeDB" id="Q97U24"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02109">
        <property type="entry name" value="arch_bact_SO_family_Moco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.420.10">
        <property type="entry name" value="Oxidoreductase, molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000572">
        <property type="entry name" value="OxRdtase_Mopterin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43032:SF4">
        <property type="entry name" value="OXIDOREDUCTASE MOLYBDOPTERIN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43032">
        <property type="entry name" value="PROTEIN-METHIONINE-SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56524">
        <property type="entry name" value="Oxidoreductase molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase molybdopterin-binding" evidence="1">
        <location>
            <begin position="23"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="199" mass="23617" checksum="4BE538F6E02C4867" modified="2001-10-01" version="1">MQEVQRPPNQKYIKNFIVYAEFGIPEVNLETYRLKVSGEVENSLSFTYEELMKLPRKEIVEDFHCVTGWSIKSVKWEGIPFKLLIEMAKVKNKVNWVMFYSLDGYTSIIPFEDLLKDNVIVALFMNGEKLPLKHGFPARPIIPHLYAWKSAKWLTEIEFIKDYVDGYWEERGYHERGNVWEEERFKGQGGKHLRRRPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U55</accession>
    <name>Q97U55_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90501">
        <property type="entry name" value="D90501"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U55"/>
    <dbReference type="STRING" id="273057.SSO3168"/>
    <dbReference type="PaxDb" id="273057-SSO3168"/>
    <dbReference type="EnsemblBacteria" id="AAK43267">
        <property type="protein sequence ID" value="AAK43267"/>
        <property type="gene ID" value="SSO3168"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3168"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3272"/>
    <dbReference type="eggNOG" id="arCOG01469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096331_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U55"/>
    <dbReference type="PhylomeDB" id="Q97U55"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051784">
        <property type="entry name" value="Nod_factor_ABC_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229:SF3">
        <property type="entry name" value="ABC-TYPE MULTIDRUG TRANSPORT SYSTEM, PERMEASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229">
        <property type="entry name" value="NODULATION PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="249" mass="27781" checksum="99A4A6E6D4B04418" modified="2001-10-01" version="1">MVVRVLKSSFLLAFTIFYGLSSIKRGFVYILTYLSIPLAELFLIYIITRGDFVKFAIVGGLISVMANNGFSLIGDLVFLRLESRLQDLLVATSIGPSDYIIGLILANLLYSSPGLILYIILAILYHVLTLSNFIPLLIDITLLLFATSSIGFFLASLVPHTRYGWGLAGLLSSILTIIPPVFYPYTLIPKSLYIPLLILPTTASAIFSQGITNLVSTSIEMLLLSFLVLLFESILFFYLSVRFSRWRER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2026-01-28" version="137" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U63</accession>
    <name>LIPA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 5">Lipoyl synthase</fullName>
            <ecNumber evidence="1 3">2.8.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Lip-syn</fullName>
            <shortName evidence="1">LS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lipoate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lipoic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Sulfur insertion protein LipA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 5" type="primary">lipA</name>
        <name type="ordered locus">SSO3158</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="Anal. Biochem." volume="351" first="44" last="49">
            <title>The activity of a thermostable lipoyl synthase from Sulfolobus solfataricus with a synthetic octanoyl substrate.</title>
            <authorList>
                <person name="Bryant P."/>
                <person name="Kriek M."/>
                <person name="Wood R.J."/>
                <person name="Roach P.L."/>
            </authorList>
            <dbReference type="PubMed" id="16500612"/>
            <dbReference type="DOI" id="10.1016/j.ab.2006.01.023"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2014" name="Biochem. J." volume="464" first="123" last="133">
            <title>Structures of lipoyl synthase reveal a compact active site for controlling sequential sulfur insertion reactions.</title>
            <authorList>
                <person name="Harmer J.E."/>
                <person name="Hiscox M.J."/>
                <person name="Dinis P.C."/>
                <person name="Fox S.J."/>
                <person name="Iliopoulos A."/>
                <person name="Hussey J.E."/>
                <person name="Sandy J."/>
                <person name="Van Beek F.T."/>
                <person name="Essex J.W."/>
                <person name="Roach P.L."/>
            </authorList>
            <dbReference type="PubMed" id="25100160"/>
            <dbReference type="DOI" id="10.1042/bj20140895"/>
        </citation>
        <scope>MUTAGENESIS OF SER-267</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine + 4 H(+) = [[Fe-S] cluster scaffold protein] + N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 5'-deoxyadenosine + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:16585"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:14568"/>
            <dbReference type="Rhea" id="RHEA-COMP:14569"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33722"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="2.8.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text evidence="3">Optimum temperature is 60 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. Lipoyl synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.8" evidence="1 3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90500">
        <property type="entry name" value="D90500"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989967.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U63"/>
    <dbReference type="SMR" id="Q97U63"/>
    <dbReference type="FunCoup" id="Q97U63">
        <property type="interactions" value="291"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3158"/>
    <dbReference type="PaxDb" id="273057-SSO3158"/>
    <dbReference type="EnsemblBacteria" id="AAK43259">
        <property type="protein sequence ID" value="AAK43259"/>
        <property type="gene ID" value="SSO3158"/>
    </dbReference>
    <dbReference type="GeneID" id="1453178"/>
    <dbReference type="GeneID" id="44128878"/>
    <dbReference type="KEGG" id="sso:SSO3158"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3264"/>
    <dbReference type="eggNOG" id="arCOG00660">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033144_2_3_2"/>
    <dbReference type="InParanoid" id="Q97U63"/>
    <dbReference type="PhylomeDB" id="Q97U63"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00593"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016992">
        <property type="term" value="F:lipoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000186">
        <property type="entry name" value="Lipoyl synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00206">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003698">
        <property type="entry name" value="Lipoyl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00510">
        <property type="entry name" value="lipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004019">
        <property type="entry name" value="PRK05481.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009544">
        <property type="entry name" value="PRK12928.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949">
        <property type="entry name" value="LIPOYL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949:SF0">
        <property type="entry name" value="LIPOYL SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005963">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00271">
        <property type="entry name" value="lipoyl_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01058">
        <property type="entry name" value="lipoyl_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000102394" description="Lipoyl synthase">
        <location>
            <begin position="1"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="43"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Loss of activity. Does not produce lipoyl product." evidence="4">
        <original>S</original>
        <variation>A</variation>
        <variation>C</variation>
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="16500612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="25100160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="16500612"/>
        </source>
    </evidence>
    <sequence length="285" mass="32111" checksum="B48A93B5C7FE13EC" modified="2001-10-01" version="1">MEKKVQIAVYENENFKRVAEIVKKKSIATVCEEALCPNIMECWGSGTATFMIMGSICTRGCRFCYVLKGKPSPLDDEEPKRVAEAVKEMELDYVVITSVDRDDLSDGGAQHFANVVKTVKELNPGIIVEVLTPDFRGNIDAVKKVIDAGVDVFAHNVETVRRLTPIVRDPRASYEQSLNVLKYAENVIKKSSILLGLGETWDEIVETMRDLRSVGVSILVLSQYMRPSRKQLEVKKRYTLEEFKELEEIAYSMGFSAVISLPLARTSYKAKEAYFRAIENAKNHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U68</accession>
    <name>Q97U68_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Second ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO3153</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90499">
        <property type="entry name" value="G90499"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924148.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U68"/>
    <dbReference type="STRING" id="273057.SSO3153"/>
    <dbReference type="PaxDb" id="273057-SSO3153"/>
    <dbReference type="EnsemblBacteria" id="AAK43254">
        <property type="protein sequence ID" value="AAK43254"/>
        <property type="gene ID" value="SSO3153"/>
    </dbReference>
    <dbReference type="GeneID" id="1453173"/>
    <dbReference type="KEGG" id="sso:SSO3153"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3259"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="InParanoid" id="Q97U68"/>
    <dbReference type="PhylomeDB" id="Q97U68"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF22">
        <property type="entry name" value="TNPB-LIKE PROTEIN MJ1635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="187"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="308"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK43254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="423" mass="49977" checksum="6D9FBC77CA8C2A03" modified="2001-10-01" version="1">MLKNLRIRKFEPEEEYVYFTYSIKNDKREKSKELIKEYGTLLQKAIDYLWSLTKIQVRKNGNYKITLPKKKEVYKPLREELEKINHLASHYVDKAINDAFSILKSWRKRAIKGRASIEKPGVKNAYVRIKTTLRKVVGESVRITVRPYEYITFSWSNLWFSRRVRELELGEPIIKEEKVYLPFRYKLPWATPLNFLAIDSNLYTLDAYDGEKFITISLKQLYSLKYSMEVKRAKVQSFASKHTKRGRELLRKYSHRERNRVLDFVRKFVNALLDLYPMTFFAVEKLSKESMFKDANGSLSRKISRTVWGSIHRVLKYKAPLYGSFVKEVNPHFTSRSCPRCGFVSRKVGKTFECERCGFKLDRQLNASLNIYLKMCGFPHIRDIPRVWVGVIPLMGRRGMNGFPRDFGEAQGLRIDIKYHEIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U71</accession>
    <name>Q97U71_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Permease, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO3149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90499">
        <property type="entry name" value="C90499"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991648.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U71"/>
    <dbReference type="FunCoup" id="Q97U71">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3149"/>
    <dbReference type="PaxDb" id="273057-SSO3149"/>
    <dbReference type="EnsemblBacteria" id="AAK43250">
        <property type="protein sequence ID" value="AAK43250"/>
        <property type="gene ID" value="SSO3149"/>
    </dbReference>
    <dbReference type="GeneID" id="1453169"/>
    <dbReference type="KEGG" id="sso:SSO3149"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3254"/>
    <dbReference type="eggNOG" id="arCOG00132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025894_0_2_2"/>
    <dbReference type="InParanoid" id="Q97U71"/>
    <dbReference type="PhylomeDB" id="Q97U71"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520:SF5">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="325"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="171"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="359" mass="39194" checksum="A5E7CBE6CFCBA370" modified="2001-10-01" version="1">MKSEDFLLVLASSVGGIVWGANTVIMALYLKSLGLNPLTIGVILGSAIIVNTLLSLFWAVLGDAYGRKKFVYISRGVGGLSFLLLLITPYAYLFINQGYGLISSILAEKSGDLDKAMAYRSSLNTIFSVVGSLLPIVLNYRSIIAVDSIVTFLSLLLLIPVKENYRGTRKVTLKISSFKVLGKLSTEAIIGLGAGVLLPMLSLWFNLRFHATASSLSPIYAISEITLALGTLTSPFLAKTLGRIRAIVITHFAAIALLFIMPFSTSLFIAGVIYVMRNVLMNSTGPLMNSLVFKVVKEEERSRVNSMLQLLDAIPRSLGPNLTGYLFYIGNLNVPFFITGSLYLVATILFYNFFKDIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U76</accession>
    <name>Q97U76_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">SSO3147</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Metabolite:H+ Symporter (MHS) family (TC 2.A.1.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90498">
        <property type="entry name" value="F90498"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924144.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U76"/>
    <dbReference type="STRING" id="273057.SSO3147"/>
    <dbReference type="PaxDb" id="273057-SSO3147"/>
    <dbReference type="EnsemblBacteria" id="AAK43245">
        <property type="protein sequence ID" value="AAK43245"/>
        <property type="gene ID" value="SSO3147"/>
    </dbReference>
    <dbReference type="GeneID" id="1453164"/>
    <dbReference type="KEGG" id="sso:SSO3147"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3251"/>
    <dbReference type="eggNOG" id="arCOG02690">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_6_2"/>
    <dbReference type="InParanoid" id="Q97U76"/>
    <dbReference type="PhylomeDB" id="Q97U76"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000960">
        <property type="entry name" value="Major facilitator superfamily MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000973">
        <property type="entry name" value="Major facilitator superfamily MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051084">
        <property type="entry name" value="H+-coupled_symporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43528">
        <property type="entry name" value="ALPHA-KETOGLUTARATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43528:SF1">
        <property type="entry name" value="ALPHA-KETOGLUTARATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="354"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="15"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK43245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="417" mass="45782" checksum="1AC969FE4BD0BA42" modified="2001-10-01" version="1">MLNEMSSLSSREIKLIFLNFMSYTFLVYNYSILLVFNSPYISQTLFKGSYVLSLLGVYGLLLIDVVMRVPGAYVLGPISDRYGRKLVTRISSLGSALPLVAVALIPDPSPLLLIILYVIQGFFTGGLSAGITVVGVEDLPERHRGWFGGSGFAVGGSAYLLASIVFFIIITVIGSYNYIEIGWRIMFLTSLLILPFGFLMPESLRFRRNKERVKSPAKLLISSYKRQFILSSTLTALWASMNALVNVMLPNFLYAVNHLSKVEIGYMSLIYSVIAIISAFMGGELSEKIGRKKVSMIGGIFGILLSPVFILMSLSSRSLIPIFVSILGFVSVFGGGGIMSYVNENFPTKIRSTGVSLSWNIGFLVGNFVPLLLITVLYLTSITLFPLVEMVTMIILGALIISVSVISHETKGNIEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UA4</accession>
    <name>Q97UA4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3-oxoacyl-(Acyl carrier protein) reductase (FabG-10)</fullName>
            <ecNumber evidence="4">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fabG-10</name>
        <name evidence="4" type="ordered locus">SSO3114</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90495">
        <property type="entry name" value="B90495"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990939.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UA4"/>
    <dbReference type="FunCoup" id="Q97UA4">
        <property type="interactions" value="364"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3114"/>
    <dbReference type="PaxDb" id="273057-SSO3114"/>
    <dbReference type="EnsemblBacteria" id="AAK43217">
        <property type="protein sequence ID" value="AAK43217"/>
        <property type="gene ID" value="SSO3114"/>
    </dbReference>
    <dbReference type="GeneID" id="7808210"/>
    <dbReference type="KEGG" id="sso:SSO3114"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3221"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_2"/>
    <dbReference type="InParanoid" id="Q97UA4"/>
    <dbReference type="PhylomeDB" id="Q97UA4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032787">
        <property type="term" value="P:monocarboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05333">
        <property type="entry name" value="BKR_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000115">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] reductase FabG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050259">
        <property type="entry name" value="SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004198">
        <property type="entry name" value="PRK05653.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009466">
        <property type="entry name" value="PRK12826.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879:SF2">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="252" mass="27517" checksum="4866C15FD486402D" modified="2001-10-01" version="1">MRKFCDLSGRIAIVTGGASGIGRAIAFKLASLGATVVLGDVKLEELKNVVNEITQQTSSKVLPLYVNVGDFNSAKEFYQKATEILGIDYVDILVNNAGINRDALFVKMTFEQWDEVMKVDLYSMFNMTKQVVDGMIRRNYGRIINMSSMSWLGNIGQANYSAAKAGVIGFTKTLARELAKYNITVNAITPGFIDTPMTRAVPEKVRQKIIERIPMGRVGSPEEVANLIAFLSSEEASYITGEVIGVTGGLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UC9</accession>
    <name>Q97UC9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3086</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90492">
        <property type="entry name" value="A90492"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UC9"/>
    <dbReference type="STRING" id="273057.SSO3086"/>
    <dbReference type="PaxDb" id="273057-SSO3086"/>
    <dbReference type="DNASU" id="1453111"/>
    <dbReference type="EnsemblBacteria" id="AAK43192">
        <property type="protein sequence ID" value="AAK43192"/>
        <property type="gene ID" value="SSO3086"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3086"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3194"/>
    <dbReference type="eggNOG" id="arCOG07339">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2166965_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UC9"/>
    <dbReference type="PhylomeDB" id="Q97UC9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DsrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="115" mass="13148" checksum="E744D2A664CE5322" modified="2001-10-01" version="1">MVSAMVKKIAYIVESNLGSYILGQMVVPQLEENRHNAEVIGMFFIHDNVYILMKDNPLAERLGKLSRERGIYLQACDQCVYMRNLADKLIEEAKIGCFPDFYAKVLDTVDMIITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UI8</accession>
    <name>Q97UI8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Xylose isomerase-like TIM barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90484">
        <property type="entry name" value="C90484"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993108.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UI8"/>
    <dbReference type="STRING" id="273057.SSO3029"/>
    <dbReference type="PaxDb" id="273057-SSO3029"/>
    <dbReference type="DNASU" id="1453049"/>
    <dbReference type="EnsemblBacteria" id="AAK43130">
        <property type="protein sequence ID" value="AAK43130"/>
        <property type="gene ID" value="SSO3029"/>
    </dbReference>
    <dbReference type="GeneID" id="1453049"/>
    <dbReference type="KEGG" id="sso:SSO3029"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3124"/>
    <dbReference type="eggNOG" id="arCOG01900">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050006_2_2_2"/>
    <dbReference type="InParanoid" id="Q97UI8"/>
    <dbReference type="PhylomeDB" id="Q97UI8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050312">
        <property type="entry name" value="IolE/XylAMocC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110">
        <property type="entry name" value="HYDROXYPYRUVATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110:SF41">
        <property type="entry name" value="INOSOSE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="1">
        <location>
            <begin position="21"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="268" mass="31218" checksum="69E840AE13F16FFF" modified="2001-10-01" version="1">MDWKLGIISDEISQDFEHAVKVIKELGASYVEVRNLWNKNVTQLLDSEFSEMKRVVEKYGLIISNLDSPSFKIYIDDEKGYKEHLQILRKVIELSKKLDIAYTRIFTFWYQGELRYYIDKLAERFNSAIDIAQSEGVILVIENEYSCFVGTGKELRTFLDKIRTKWVKVLWDPGNAFFARETPYPDGYELIKGDIMHLHIKDAMVKDGHFIWMPVGKGMINYKEQFRALRGSAHVISLETHYRNSANDPEASTRESFNGIIKILKELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UP9</accession>
    <name>Q97UP9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90475">
        <property type="entry name" value="D90475"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UP9"/>
    <dbReference type="STRING" id="273057.SSO2956"/>
    <dbReference type="PaxDb" id="273057-SSO2956"/>
    <dbReference type="EnsemblBacteria" id="AAK43059">
        <property type="protein sequence ID" value="AAK43059"/>
        <property type="gene ID" value="SSO2956"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2956"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3046"/>
    <dbReference type="eggNOG" id="arCOG06508">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134214_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UP9"/>
    <dbReference type="PhylomeDB" id="Q97UP9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase InsH N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="194" mass="22952" checksum="7825B5380BF58612" modified="2001-10-01" version="1">MVKAISTEKDLLLKVDKSFPWETFRSKLKSLYSKKPKWNVISLLKVLLIKFVFDISWNNLEGEIRDSKRFMDFLGGKVPPKSTVFFFYKKLQQTVIQEGETMRTTLMDELNKALDKVISEYREKGFELEVGREKNDRFEYYHIIDIFFREAYPGKRSLQNGLEKISLDLKEMGFNDVFLLMGYTTSKLKNFHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UQ9</accession>
    <name>Q97UQ9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">First ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO2943</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2009-08" db="PDB data bank">
            <title>Crystal structure of First ORF in transposon ISC1904 from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Chang C."/>
                <person name="Bigelow L."/>
                <person name="Bearden J."/>
                <person name="Joachimiak A."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 55-194</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90473">
        <property type="entry name" value="H90473"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924046.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3ILX">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B=55-194"/>
    </dbReference>
    <dbReference type="PDBsum" id="3ILX"/>
    <dbReference type="AlphaFoldDB" id="Q97UQ9"/>
    <dbReference type="SMR" id="Q97UQ9"/>
    <dbReference type="STRING" id="273057.SSO2943"/>
    <dbReference type="PaxDb" id="273057-SSO2943"/>
    <dbReference type="EnsemblBacteria" id="AAK43047">
        <property type="protein sequence ID" value="AAK43047"/>
        <property type="gene ID" value="SSO2943"/>
    </dbReference>
    <dbReference type="GeneID" id="1452966"/>
    <dbReference type="KEGG" id="sso:SSO2943"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3031"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="InParanoid" id="Q97UQ9"/>
    <dbReference type="PhylomeDB" id="Q97UQ9"/>
    <dbReference type="EvolutionaryTrace" id="Q97UQ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04762">
        <property type="entry name" value="HTH_MerR-trunc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1660.10:FF:000013">
        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2170:FF:000002">
        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1390:FF:000002">
        <property type="entry name" value="ORF1 in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="8" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="5">
        <location>
            <begin position="55"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="8">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="3ILX"/>
        </source>
    </evidence>
    <sequence length="194" mass="22642" checksum="B01BF8E4E8DAC725" modified="2001-10-01" version="1">MLRPKEACQRLGISYATLREYVKKGYIKPVILQSGKWRFREEDIERLMGIIRKRKVILYARVSSNTQKDDLANQVKYLEEQVKEYDLVITDIGSGLNMKRKGFLKLLRMILNNEVSRVITAYPDRLVRFGFEILEEVCKAHNCEIVVLNQEDKTPEEELVEDLATILVSFSGKLHGMRSQKYEKVKKCAEELKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UU8</accession>
    <name>Q97UU8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">First ORF in transposon ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2894</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90468">
        <property type="entry name" value="C90468"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UU8"/>
    <dbReference type="STRING" id="273057.SSO2894"/>
    <dbReference type="PaxDb" id="273057-SSO2894"/>
    <dbReference type="EnsemblBacteria" id="AAK43002">
        <property type="protein sequence ID" value="AAK43002"/>
        <property type="gene ID" value="SSO2894"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2894"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2982"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066381_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UU8"/>
    <dbReference type="PhylomeDB" id="Q97UU8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="3">
        <location>
            <begin position="193"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="249" mass="28319" checksum="D8AB05B86DD8B09C" modified="2001-10-01" version="1">MEAPVAGIDVSKDKLVVYFQGKYYEFQNNKQGFEGVKQVLPKGCKVAIESTGVYHINLAKYLGKEYDVRIINPLILKKFKDIRGKKSDKNDKKLAELVVSMGSEFTTSEAKELTSQWDFITRSIVRVKNRLRRDLILLGYKDSLSKKNLNEVLRGEDNIVLSEVRFLLDELERLEVRKREIEEELEDLVPKDSLIFTIPGIGRTLGCIILARVGDVKRFGDKKRFVAYCGLDPLVESSGKSVVSRGISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UW9</accession>
    <name>Q97UW9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3-hydroxybutyryl-CoA epimerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2872</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90465">
        <property type="entry name" value="D90465"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989827.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UW9"/>
    <dbReference type="STRING" id="273057.SSO2872"/>
    <dbReference type="PaxDb" id="273057-SSO2872"/>
    <dbReference type="EnsemblBacteria" id="AAK42979">
        <property type="protein sequence ID" value="AAK42979"/>
        <property type="gene ID" value="SSO2872"/>
    </dbReference>
    <dbReference type="GeneID" id="15298823"/>
    <dbReference type="KEGG" id="sso:SSO2872"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2958"/>
    <dbReference type="eggNOG" id="arCOG01283">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119412_2_1_2"/>
    <dbReference type="InParanoid" id="Q97UW9"/>
    <dbReference type="PhylomeDB" id="Q97UW9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="ChsH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="ChsH2_Znr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF6">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_ChsH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="2">
        <location>
            <begin position="64"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="ChsH2 C-terminal OB-fold" evidence="1">
        <location>
            <begin position="101"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20517" checksum="F7AF1573E556777E" modified="2001-10-01" version="1">MSLNEIRDKMQKEISQSLLMLDNAIKTTKLPIVNEQKTNNPLWIDVREIDLRYQIPVKRIFKFFEGLREGKILATKCPKCGSIYFPPQDDCPKCKISNLEWIEMPKEGEIVAYTVVNVKPPSFSHYQDYIVGIARMSNGVNVLGWVRAKEVRVGMKVKLEVIERKPEGYLTYELVPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UX9</accession>
    <name>Q97UX9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2861</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90464">
        <property type="entry name" value="B90464"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989840.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UX9"/>
    <dbReference type="FunCoup" id="Q97UX9">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2861"/>
    <dbReference type="PaxDb" id="273057-SSO2861"/>
    <dbReference type="EnsemblBacteria" id="AAK42969">
        <property type="protein sequence ID" value="AAK42969"/>
        <property type="gene ID" value="SSO2861"/>
    </dbReference>
    <dbReference type="GeneID" id="1452888"/>
    <dbReference type="KEGG" id="sso:SSO2861"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2948"/>
    <dbReference type="eggNOG" id="arCOG00777">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_3_3_2"/>
    <dbReference type="InParanoid" id="Q97UX9"/>
    <dbReference type="PhylomeDB" id="Q97UX9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000113">
        <property type="entry name" value="Thioesterase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660:SF1">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660">
        <property type="entry name" value="THIOESTERASE SUPERFAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="3">
        <location>
            <begin position="51"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="147" mass="16640" checksum="295799A610DF73A7" modified="2001-10-01" version="1">MDSQNYDIEKILQRYDSDYVFHYLGVKILEIKEGYAKTQIPFKKELTRRGNILNGGMIVASIDYTGGLAVLSINDGMDQVTQELKVNFLEPMYRGPFTVEANVIRKGRTAVTVEITFKDVEGSLGAIGLGTWYIIRDKKINMNVQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V06</accession>
    <name>Q97V06_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">AAA family ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2831</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90460">
        <property type="entry name" value="F90460"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990888.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V06"/>
    <dbReference type="STRING" id="273057.SSO2831"/>
    <dbReference type="PaxDb" id="273057-SSO2831"/>
    <dbReference type="EnsemblBacteria" id="AAK42941">
        <property type="protein sequence ID" value="AAK42941"/>
        <property type="gene ID" value="SSO2831"/>
    </dbReference>
    <dbReference type="GeneID" id="1452860"/>
    <dbReference type="KEGG" id="sso:SSO2831"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2916"/>
    <dbReference type="eggNOG" id="arCOG01308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_8_1_2"/>
    <dbReference type="InParanoid" id="Q97V06"/>
    <dbReference type="PhylomeDB" id="Q97V06"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003724">
        <property type="entry name" value="AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001025">
        <property type="entry name" value="ATPase family, AAA domain-containing 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF199">
        <property type="entry name" value="AAA FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="91"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="358"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="585" mass="66978" checksum="7146F310057D6A5E" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V18</accession>
    <name>Q97V18_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">FIXC protein homolog (FixC)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">fixC</name>
        <name evidence="5" type="ordered locus">SSO2819</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90459">
        <property type="entry name" value="B90459"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989025.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V18"/>
    <dbReference type="FunCoup" id="Q97V18">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2819"/>
    <dbReference type="PaxDb" id="273057-SSO2819"/>
    <dbReference type="EnsemblBacteria" id="AAK42929">
        <property type="protein sequence ID" value="AAK42929"/>
        <property type="gene ID" value="SSO2819"/>
    </dbReference>
    <dbReference type="GeneID" id="1452848"/>
    <dbReference type="KEGG" id="sso:SSO2819"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2904"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050977_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V18"/>
    <dbReference type="PhylomeDB" id="Q97V18"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039651">
        <property type="entry name" value="FixC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43624">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-QUINONE OXIDOREDUCTASE YDIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43624:SF2">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-QUINONE OXIDOREDUCTASE YDIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12831">
        <property type="entry name" value="FAD_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="395" mass="43305" checksum="4E7D420707397A0C" modified="2001-10-01" version="1">MKFDIVVVGAGPAGSSAAITAARGGAKVLLLERGPEPGSKNVSGAMIRLEDFSSVYDIASIPIERKVKNVDLILLSDSNRTRISVNVESNLATVARLKLDKWMAQQAEKAGALLITKTTVLGVEREGNEYKISTDRGEITADRVVLAEGVNALVSMKANIRPDLTPDHAAQTVKEVYSLNKDEINKRFGFKADDEGISWRILGTDPVPYAGFLYVYKDAVAIGAGIPMKILIKRKITPYTVLDEVKERLNFNELVKGASLREYSAKVIPEYGFPSWKACSGKVYLAGDAIGLVDPLTFNGIGPAVASGVVAGKAALESYECNKYEYELMKNKEVRRVVNARPLVKELIEEENFKYYVKTINDLLHGWVYSDFSKVKLDTSKLLKHLLLGMGVLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V32</accession>
    <name>Q97V32_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alcohol dehydrogenase (Zn containing) (Adh-12)</fullName>
            <ecNumber evidence="7">1.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">adh-12</name>
        <name evidence="7" type="ordered locus">SSO2800</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90457">
        <property type="entry name" value="B90457"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V32"/>
    <dbReference type="FunCoup" id="Q97V32">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2800"/>
    <dbReference type="PaxDb" id="273057-SSO2800"/>
    <dbReference type="EnsemblBacteria" id="AAK42913">
        <property type="protein sequence ID" value="AAK42913"/>
        <property type="gene ID" value="SSO2800"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2800"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2886"/>
    <dbReference type="eggNOG" id="arCOG01458">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_4_2"/>
    <dbReference type="InParanoid" id="Q97V32"/>
    <dbReference type="PhylomeDB" id="Q97V32"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08264">
        <property type="entry name" value="Zn_ADH_like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="6">
        <location>
            <begin position="11"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="331" mass="36852" checksum="A3A1F1F4EE177A61" modified="2001-10-01" version="1">MMKALTFDKNGIENLKFTDYKNPEIGNHEVLIRVKLAGVNPVDYYTVERLKVNPIPHIPGVEFSGEVVKVGDHVKSVSVGDRVTIYGRIFDGTCDMCMAGYETVCRNGGRIGVDANGGWAEYVAVEEKYVFKLPNEYTWEMGSSLTVAALTAYHALKEAQLSPSQTLVVFGASGNTGMFLVQLGKKFGAKVIAVSRKSWLRGYGADFVVDYNEVEEKVKEITNGKMADVVINSLGEQFWDKSFSVLGVRGKLVTFGTLLGGNVKVDLSQLYSKHISILGVNRGNRKDFIELLDICKDCKVKTWKIFKLEEGKEALKELFSKERDGRIFLYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V98</accession>
    <name>Q97V98_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF433 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2736</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90448">
        <property type="entry name" value="H90448"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990280.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V98"/>
    <dbReference type="STRING" id="273057.SSO2736"/>
    <dbReference type="PaxDb" id="273057-SSO2736"/>
    <dbReference type="EnsemblBacteria" id="AAK42847">
        <property type="protein sequence ID" value="AAK42847"/>
        <property type="gene ID" value="SSO2736"/>
    </dbReference>
    <dbReference type="GeneID" id="1452766"/>
    <dbReference type="KEGG" id="sso:SSO2736"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2816"/>
    <dbReference type="eggNOG" id="arCOG07520">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1472148_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V98"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007367">
        <property type="entry name" value="DUF433"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04255">
        <property type="entry name" value="DUF433"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="21662" checksum="054786C609654DD6" modified="2001-10-01" version="1">MELPGYDYIIVYKDIHFGRPFIAGTLIRPENVLYELAKDKTFDEVSKAFYNQINFKQIRECIKYAIDVMKILKYYKKVKPKVPRRLKRKLGPTNYTFIDKDDDNNSSKYDPKIKNSSTKVVDVLNKLYEGKEISQVAEELNIPKEAVIESILYSASLVDDFHLSLSAFEDPASVVIESFNYIRKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VC5</accession>
    <name>Q97VC5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrogenase expression/formation factor related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2708</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90445">
        <property type="entry name" value="E90445"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988637.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VC5"/>
    <dbReference type="STRING" id="273057.SSO2708"/>
    <dbReference type="PaxDb" id="273057-SSO2708"/>
    <dbReference type="DNASU" id="1452739"/>
    <dbReference type="EnsemblBacteria" id="AAK42820">
        <property type="protein sequence ID" value="AAK42820"/>
        <property type="gene ID" value="SSO2708"/>
    </dbReference>
    <dbReference type="GeneID" id="1452739"/>
    <dbReference type="KEGG" id="sso:SSO2708"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2790"/>
    <dbReference type="eggNOG" id="arCOG00636">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041631_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VC5"/>
    <dbReference type="PhylomeDB" id="Q97VC5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06061">
        <property type="entry name" value="PurM-like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011854">
        <property type="entry name" value="HypE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303:SF4">
        <property type="entry name" value="HYDROGENASE EXPRESSION_FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303">
        <property type="entry name" value="HYDROGENASE ISOENZYMES FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005644">
        <property type="entry name" value="Hdrgns_mtr_HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="2">
        <location>
            <begin position="35"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="3">
        <location>
            <begin position="157"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="341" mass="38182" checksum="4D410194503B5840" modified="2001-10-01" version="1">MHLGKINEDVFNKVIYPHLGERRKEVVVGPQHGVDTGAIDLQDGRVLVVKTDPVFIVPQFGFKKAAWFAVHILASDVMTSGIPPEYAVIDLNLPPRIKDEEFEEMWIGIHEALKEIGVMVVGGHTGVYEGTDYPMVGGFTMFGIGEKERLGMPSKVKVGDSVIMTKGPAIEATGLLTNLYPEYFKQRLSNEIFNEAYNMYWKMSCWKDGLIASRIGIHMMHDATEGGVFGALTEVARASSKGMRIYEERFFINRAVREVTKLVNIDPWISISEGTMIIVTDKGEEVRNTLIREGIEAEIIGEIVDQSGEVTMVKKDGSRVKIEHPLEDPFWRAFFDLASKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VD2</accession>
    <name>Q97VD2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TenA family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2700</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2013-07" db="PDB data bank">
            <title>Crystal structure of a TENA/THI-4 domain-containing protein (SSO2700) from Sulfolobus solfataricus P2 at 2.34 A resolution.</title>
            <authorList>
                <consortium name="Joint Center for Structural Genomics (JCSG)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.34 ANGSTROMS) IN COMPLEX WITH ZN(2+)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90444">
        <property type="entry name" value="E90444"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988625.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="4LQX">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.34 A"/>
        <property type="chains" value="A/B=1-303"/>
    </dbReference>
    <dbReference type="PDBsum" id="4LQX"/>
    <dbReference type="AlphaFoldDB" id="Q97VD2"/>
    <dbReference type="SMR" id="Q97VD2"/>
    <dbReference type="STRING" id="273057.SSO2700"/>
    <dbReference type="PaxDb" id="273057-SSO2700"/>
    <dbReference type="DNASU" id="1452731"/>
    <dbReference type="EnsemblBacteria" id="AAK42812">
        <property type="protein sequence ID" value="AAK42812"/>
        <property type="gene ID" value="SSO2700"/>
    </dbReference>
    <dbReference type="GeneID" id="1452731"/>
    <dbReference type="KEGG" id="sso:SSO2700"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2780"/>
    <dbReference type="eggNOG" id="arCOG06031">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_955157_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VD2"/>
    <dbReference type="EvolutionaryTrace" id="Q97VD2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19370">
        <property type="entry name" value="TenA_PqqC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039068">
        <property type="entry name" value="PqqC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017004">
        <property type="entry name" value="UCP032676_PqqC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032553">
        <property type="entry name" value="UCP032676_Znf-C2H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40279:SF3">
        <property type="entry name" value="4-AMINOBENZOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40279">
        <property type="entry name" value="PQQC-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16293">
        <property type="entry name" value="zf-C2H2_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032676">
        <property type="entry name" value="UCP032676_PQQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="6" id="KW-0002">3D-structure</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="UCP032676 C2H2 type zinc-finger" evidence="3">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Thiaminase-2/PQQC" evidence="2">
        <location>
            <begin position="69"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="4"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="4LQX"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="35201" checksum="FEE07D611BD4180B" modified="2001-10-01" version="1">MPLCPSCEMKFNSWEDLAKHMDLIANTNSDKSHVMWLNRNISMKRMEVNELANALERFFSTPNSLSMWIRTRFIERFYGDNPHPFIVAMQNPTKGVLLGYVIEHQHFLKNWVKVLSSIVFKTDKDDVLQYELENISVEFIGYNGRPAHYELLLRMGEALGMPREKILSTQPLPSTQSAIKTWRKIAESKTWLETMASMHSLELVADRSLVKYGAKLPYFNPEILSSDEYPQAVKDFLREGYEADVSHAGEALEMVEKYTEEMEMKEQVQITVLKSFDAFSKYLLARLERGFEIEPSLLKRVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VE2</accession>
    <name>Q97VE2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zinc-ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2686</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90443">
        <property type="entry name" value="C90443"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923956.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VE2"/>
    <dbReference type="STRING" id="273057.SSO2686"/>
    <dbReference type="PaxDb" id="273057-SSO2686"/>
    <dbReference type="EnsemblBacteria" id="AAK42802">
        <property type="protein sequence ID" value="AAK42802"/>
        <property type="gene ID" value="SSO2686"/>
    </dbReference>
    <dbReference type="GeneID" id="1452721"/>
    <dbReference type="KEGG" id="sso:SSO2686"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2765"/>
    <dbReference type="eggNOG" id="arCOG07264">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1145258_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VE2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="Zn_Ribbon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc-ribbon" evidence="2">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="256" mass="27558" checksum="E44D20B7DAB6B001" modified="2001-10-01" version="1">MPKQCPKCGYINPDDANYCSKCGYPLQYQPIISTNPPSTPSPTSPNPLQPDRLSTSFNILTKNLSIIFPPIIMLIIEVILLALFGAITAGISLISPVAFTVTALIFSIIIGIVDAIIFSITVHTTTYMARDAVMGAQLNLNNAFTNARNTLSRLYPIIAILIVLGILLGLSRSLGLGWIIMGLVGVLLYIVSAATILNRPMSLSETINWYSRAFGVDAGGAVVILIGSLLSLIPIVNIFAIPYTSILTYLMVRDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VQ8</accession>
    <name>Q97VQ8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleotidyl transferase AbiEii/AbiGii toxin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90428">
        <property type="entry name" value="E90428"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992728.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VQ8"/>
    <dbReference type="STRING" id="273057.SSO2555"/>
    <dbReference type="PaxDb" id="273057-SSO2555"/>
    <dbReference type="EnsemblBacteria" id="AAK42684">
        <property type="protein sequence ID" value="AAK42684"/>
        <property type="gene ID" value="SSO2555"/>
    </dbReference>
    <dbReference type="GeneID" id="1454011"/>
    <dbReference type="KEGG" id="sso:SSO2555"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2637"/>
    <dbReference type="eggNOG" id="arCOG06048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093138_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VQ8"/>
    <dbReference type="PhylomeDB" id="Q97VQ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="29439" checksum="BBDAF818E3A7FF0C" modified="2001-10-01" version="1">MTLTKEQLLNRGIEVIDKAENEGITLRVIGGAAIALIAKKGSELYPRVYKDIDYFGLSSQSTKISRFLEGIGLIPNKRFNALHGHIRLMFFDPIINSTVDVFLDEFVMCHKLVLKDRLRIMKYTIPTSDLFLTKMQIIQLTENDKKDIAALLYDVELGERDDEKTMNYNYIAKILSEDWGFYKTYTINHDRVLEFMKNDKNREIIEPKLVKLREIIEKHPKSIKWKMRAKIGEKVKWYEEPEEVNTNFTQQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2026-01-28" version="167" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W02</accession>
    <name>DPO4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>DNA polymerase IV</fullName>
            <shortName>Pol IV</shortName>
            <ecNumber>2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">dbh</name>
        <name type="synonym">dpo4</name>
        <name type="ordered locus">SSO2448</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Nucleic Acids Res." volume="29" first="4607" last="4616">
            <title>Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4): an archaeal DinB-like DNA polymerase with lesion-bypass properties akin to eukaryotic pol eta.</title>
            <authorList>
                <person name="Boudsocq F."/>
                <person name="Iwai S."/>
                <person name="Hanaoka F."/>
                <person name="Woodgate R."/>
            </authorList>
            <dbReference type="PubMed" id="11713310"/>
            <dbReference type="DOI" id="10.1093/nar/29.22.4607"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="19633" last="19638">
            <title>Low fidelity DNA synthesis by a Y family DNA polymerase due to misalignment in the active site.</title>
            <authorList>
                <person name="Kokoska R.J."/>
                <person name="Bebenek K."/>
                <person name="Boudsocq F."/>
                <person name="Woodgate R."/>
                <person name="Kunkel T.A."/>
            </authorList>
            <dbReference type="PubMed" id="11919199"/>
            <dbReference type="DOI" id="10.1074/jbc.M202021200"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2001" name="Cell" volume="107" first="91" last="102">
            <title>Crystal structure of a Y-family DNA polymerase in action: a mechanism for error-prone and lesion-bypass replication.</title>
            <authorList>
                <person name="Ling H."/>
                <person name="Boudsocq F."/>
                <person name="Woodgate R."/>
                <person name="Yang W."/>
            </authorList>
            <dbReference type="PubMed" id="11595188"/>
            <dbReference type="DOI" id="10.1016/S0092-8674(01)00515-3"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF TERNARY COMPLEX WITH DNA AND AN INCOMING NUCLEOTIDE</scope>
        <scope>MUTAGENESIS OF 105-ASP-GLU-106</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2009" name="Mol. Microbiol." volume="71" first="678" last="691">
            <title>Structural insight into recruitment of translesion DNA polymerase Dpo4 to sliding clamp PCNA.</title>
            <authorList>
                <person name="Xing G."/>
                <person name="Kirouac K."/>
                <person name="Shin Y.J."/>
                <person name="Bell S.D."/>
                <person name="Ling H."/>
            </authorList>
            <dbReference type="PubMed" id="19054331"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.2008.06553.x"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 1-244 IN COMPLEX WITH PCNA</scope>
        <scope>INTERACTION WITH PCNA1</scope>
        <scope>SUBUNIT</scope>
        <scope>DNA-BINDING</scope>
        <scope>MUTAGENESIS OF 342-GLU--THR-352</scope>
    </reference>
    <comment type="function">
        <text>Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. It is involved in translesional synthesis.</text>
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        <reaction>
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
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        <cofactor>
            <name>Mg(2+)</name>
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        <text>Binds 2 magnesium ions per subunit.</text>
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    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text>Highly active from 35 to 95 degrees Celsius. Thermostable.</text>
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    <comment type="subunit">
        <text evidence="2">Monomer. Interacts with the PCNA heterotrimer via PCNA1.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
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    <comment type="domain">
        <text>The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.</text>
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        <text evidence="3">Belongs to the DNA polymerase type-Y family.</text>
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    <evidence type="ECO:0000269" key="1">
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        </source>
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        <source>
            <dbReference type="PubMed" id="19054331"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PDB" id="1JX4"/>
        </source>
    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="PDB" id="2W9B"/>
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    </evidence>
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        <source>
            <dbReference type="PDB" id="3KHH"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PDB" id="4GC7"/>
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    </evidence>
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        <source>
            <dbReference type="PDB" id="4QWC"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PDB" id="4TQR"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="12">
        <source>
            <dbReference type="PDB" id="6L97"/>
        </source>
    </evidence>
    <sequence length="352" mass="40193" checksum="C0B0AD4B3A3E87D0" modified="2001-10-01" version="1">MIVLFVDFDYFYAQVEEVLNPSLKGKPVVVCVFSGRFEDSGAVATANYEARKFGVKAGIPIVEAKKILPNAVYLPMRKEVYQQVSSRIMNLLREYSEKIEIASIDEAYLDISDKVRDYREAYNLGLEIKNKILEKEKITVTVGISKNKVFAKIAADMAKPNGIKVIDDEEVKRLIRELDIADVPGIGNITAEKLKKLGINKLVDTLSIEFDKLKGMIGEAKAKYLISLARDEYNEPIRTRVRKSIGRIVTMKRNSRNLEEIKPYLFRAIEESYYKLDKRIPKAIHVVAVTEDLDIVSRGRTFPHGISKETAYSESVKLLQKILEEDERKIRRIGVRFSKFIEAIGLDKFFDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W34</accession>
    <name>Q97W34_SACS2</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="1" type="ordered locus">SSO10348</name>
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        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
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        <lineage>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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    </dbReference>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="72" mass="8548" checksum="781D04BCCB1BAC5B" modified="2001-10-01" version="1">MRKYMTNFTIDLDSYTCSSDPLEAIEYLFNNNNVIFKIKSANPYFEIIKDRYTINIIKQEGDTIYFIIRYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WA2</accession>
    <name>Q97WA2_SACS2</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
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                <person name="Schenk M.E."/>
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                <person name="Doolittle W.F."/>
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                <person name="Garrett R.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
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    </dbReference>
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        <location>
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            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAK42486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="255" mass="29529" checksum="291B60E7C406F890" modified="2001-10-01" version="1">MIRINVVGFKGGSGKSTISYHLARQLSEYYNVVLVDKTYSGTISRIYNINNNIFSFLKGRNELFYINKNNLSVINMSFLNEDDLNGIDFNAFKYLYSKLTKDSDIVIVDHSSIPHDFATEIELKAFMENFRNFAYNTVLVLSGDEIPIKKYLNYTVLLNDFVKNYAEKILGISLPTDVKFLKIVAVIINKILKGQESKLEEFIRGDEILQRSARFIIPYYLSLTQRHFKDIDPPKEISDITRYILDFLREPTSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WC4</accession>
    <name>Q97WC4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cobalamin biosynthesis precorrin isomerase, putative (CbiC)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cbiC</name>
        <name evidence="8" type="ordered locus">SSO2308</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CobH/CbiC family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99401">
        <property type="entry name" value="A99401"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991805.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WC4"/>
    <dbReference type="FunCoup" id="Q97WC4">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2308"/>
    <dbReference type="PaxDb" id="273057-SSO2308"/>
    <dbReference type="EnsemblBacteria" id="AAK42464">
        <property type="protein sequence ID" value="AAK42464"/>
        <property type="gene ID" value="SSO2308"/>
    </dbReference>
    <dbReference type="GeneID" id="1453791"/>
    <dbReference type="KEGG" id="sso:SSO2308"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2396"/>
    <dbReference type="eggNOG" id="arCOG02247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_827999_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WC4"/>
    <dbReference type="PhylomeDB" id="Q97WC4"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016993">
        <property type="term" value="F:precorrin-8X methylmutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03415">
        <property type="entry name" value="CbiX_CbiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10230">
        <property type="entry name" value="Cobalamin biosynthesis CobH/CbiC, precorrin-8X methylmutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002762">
        <property type="entry name" value="CbiX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003722">
        <property type="entry name" value="Cbl_synth_CobH/CbiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036588">
        <property type="entry name" value="CobH/CbiC_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004449">
        <property type="entry name" value="PRK05782.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43588">
        <property type="entry name" value="COBALT-PRECORRIN-8 METHYLMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43588:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-8 METHYLMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02570">
        <property type="entry name" value="CbiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01903">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63965">
        <property type="entry name" value="Precorrin-8X methylmutase CbiC/CobH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cobalamin biosynthesis precorrin-8X methylmutase CobH/CbiC" evidence="7">
        <location>
            <begin position="146"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="332" mass="36650" checksum="D19B36C99183E049" modified="2001-10-01" version="1">MDRQSNTAVILIGHGSRRETYNSDIENMINYLKEKISSPIYLTYNEFAKPDWRSLLNNVVREGYRRIVIGLVFLGRGNHVFRDIMGELGIQRLNSWVVSKISGKEVELYVTEPLSSSPLIGLALYYRLARALNALPTLEYTEDPYEIEESSMNLILSNLDVKDEREKRIIGKAIFASGNPELARYLKLTNLDAGIEAIKAGSEIVADVKMVSVGIRWRKVTCMIDNERTKELSKELGITRAAAAMRLSIGQGGKVVVIGNAPTALMETIKLVKEGIDIPFIVATPPGFTNAKESKEALIETKIPSVVLTGTYGGSGIAVAIINEIIKMAMEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WE0</accession>
    <name>Q97WE0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2286</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99399">
        <property type="entry name" value="A99399"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991829.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WE0"/>
    <dbReference type="STRING" id="273057.SSO2286"/>
    <dbReference type="PaxDb" id="273057-SSO2286"/>
    <dbReference type="EnsemblBacteria" id="AAK42448">
        <property type="protein sequence ID" value="AAK42448"/>
        <property type="gene ID" value="SSO2286"/>
    </dbReference>
    <dbReference type="GeneID" id="1453775"/>
    <dbReference type="KEGG" id="sso:SSO2286"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2379"/>
    <dbReference type="eggNOG" id="arCOG05897">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1933348_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WE0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="129" mass="15255" checksum="A38199A477CBDF8D" modified="2001-10-01" version="1">MESNLGNVINTLKRKKDKEVVIEFLKTIRKYLPPSDDFSITLWKSGIYEYILDRNGIAIMRIAEDEYLPYMTAYEKRMDYSQLPDNLVNNLDWKGILRQLRDIALEYAKRDKSFDKIADMVNNVILENL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-11-13" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WG4</accession>
    <name>CAPPA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoenolpyruvate carboxylase</fullName>
            <shortName evidence="1">PEPC</shortName>
            <shortName evidence="1">PEPCase</shortName>
            <ecNumber evidence="1">4.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ppcA</name>
        <name type="ordered locus">SSO2256</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="J. Bacteriol." volume="186" first="7754" last="7762">
            <title>Identification and functional verification of archaeal-type phosphoenolpyruvate carboxylase, a missing link in archaeal central carbohydrate metabolism.</title>
            <authorList>
                <person name="Ettema T.J."/>
                <person name="Makarova K.S."/>
                <person name="Jellema G.L."/>
                <person name="Gierman H.J."/>
                <person name="Koonin E.V."/>
                <person name="Huynen M.A."/>
                <person name="de Vos W.M."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="15516590"/>
            <dbReference type="DOI" id="10.1128/JB.186.22.7754-7762.2004"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>oxaloacetate + phosphate = phosphoenolpyruvate + hydrogencarbonate</text>
            <dbReference type="Rhea" id="RHEA:28370"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.1.1.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1 2">Mg(2+) cannot be replaced by Mn(2+).</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Allosterically inhibited by L-aspartate and L-malate. PEPC activity is not affected by allosteric activators of E.coli PEPC such as glucose 6-phosphate, fructose 1,6-bisphosphate, and acetyl coenzyme A.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">0.09 mM for phosphoenolpyruvate</KM>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 8.0.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 85 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PEPCase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.31" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90395">
        <property type="entry name" value="H90395"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991534.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WG4"/>
    <dbReference type="SMR" id="Q97WG4"/>
    <dbReference type="FunCoup" id="Q97WG4">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2256"/>
    <dbReference type="PaxDb" id="273057-SSO2256"/>
    <dbReference type="EnsemblBacteria" id="AAK42423">
        <property type="protein sequence ID" value="AAK42423"/>
        <property type="gene ID" value="SSO2256"/>
    </dbReference>
    <dbReference type="GeneID" id="1453750"/>
    <dbReference type="GeneID" id="44127989"/>
    <dbReference type="KEGG" id="sso:SSO2256"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2350"/>
    <dbReference type="eggNOG" id="arCOG04435">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_517433_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WG4"/>
    <dbReference type="PhylomeDB" id="Q97WG4"/>
    <dbReference type="BRENDA" id="4.1.1.31">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008964">
        <property type="term" value="F:phosphoenolpyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015977">
        <property type="term" value="P:carbon fixation"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01904">
        <property type="entry name" value="PEPcase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007566">
        <property type="entry name" value="PEP_COase_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02751">
        <property type="entry name" value="PEPCase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14010">
        <property type="entry name" value="PEPcase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006677">
        <property type="entry name" value="UCP006677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0021">Allosteric enzyme</keyword>
    <keyword id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000309615" description="Phosphoenolpyruvate carboxylase">
        <location>
            <begin position="1"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15516590"/>
        </source>
    </evidence>
    <sequence length="511" mass="58772" checksum="90E0795BB2EC2105" modified="2001-10-01" version="1">MRIIPRTMSTQHPDNAKVPEWAKSEVIEGEDEVKEAFLAYSMYGVHEVMWDAEGKDVDTHVVRKLLSNYPDYFREHILGKDLFLTYRLPNPKVEGADRKVFAETMESIPITYDLAEKFYGNGITIPVFEVILPMTTSSLEIISVARYYEKAVANEDELELYDGVKVKDLVGEIYPKVIEVIPLVEDRDSLQNINNIVEGYYKVIKPKYMRVFLARSDPAMNYGMITAVLSVKIALSELYKLSESLNFEIYPIIGVGSLPFRGHLSPENYEKVLEEYKGVYTYTIQSAFKYDYDYDKVKSAISSINNSRISPARILEKYEEDVLRKITILYTERYQPIIESLANAINDVSVLLPRRRARKLHIGLFGYSRSAGKVSLPRAISFVGSLYSIGIPPELIGISSLSNLDEKEWDIFKQNYVNFKHDLQTAARFLNWESFKLIKDIWKISEDTIAKIKEDIDYAESVIGIKLGGIDYDSRKHILMSSLFLLSFKEKILQESKKYLYEMALIRRSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WL7</accession>
    <name>Q97WL7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KAP NTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2199</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90389">
        <property type="entry name" value="B90389"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992094.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WL7"/>
    <dbReference type="STRING" id="273057.SSO2199"/>
    <dbReference type="PaxDb" id="273057-SSO2199"/>
    <dbReference type="EnsemblBacteria" id="AAK42369">
        <property type="protein sequence ID" value="AAK42369"/>
        <property type="gene ID" value="SSO2199"/>
    </dbReference>
    <dbReference type="GeneID" id="1453696"/>
    <dbReference type="KEGG" id="sso:SSO2199"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2295"/>
    <dbReference type="eggNOG" id="arCOG07313">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_297497_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WL7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011646">
        <property type="entry name" value="KAP_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07693">
        <property type="entry name" value="KAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KAP NTPase" evidence="2">
        <location>
            <begin position="47"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="861"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="981"/>
            <end position="1008"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="1012" mass="118728" checksum="B795FCC6FBB5231E" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WM0</accession>
    <name>Q97WM0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thermopsine related protein</fullName>
            <ecNumber evidence="2">3.4.23.42</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.23.42" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90388">
        <property type="entry name" value="G90388"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WM0"/>
    <dbReference type="STRING" id="273057.SSO2194"/>
    <dbReference type="PaxDb" id="273057-SSO2194"/>
    <dbReference type="EnsemblBacteria" id="AAK42366">
        <property type="protein sequence ID" value="AAK42366"/>
        <property type="gene ID" value="SSO2194"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2194"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2290"/>
    <dbReference type="eggNOG" id="arCOG03674">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_637155_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WM0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007981">
        <property type="entry name" value="Peptidase_A5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05317">
        <property type="entry name" value="Thermopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="417"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="443" mass="48298" checksum="2664BCFB6C693A90" modified="2001-10-01" version="1">MMYKVLLIIILLLPLSMPLSIPTTSQPSALAFPSGVTSYPLNTIIYTDFVMGRINISYLNIGSSYLPGGEYFTTGNASLQLNAMVLGEYWAQNVILFHQISNNTFYATLIVNLWNLSGPFSNTTSNSLVYQGLGVICYQGPTFKVTLPLSISLFMEIVNSTLNFGYNINGQKGIYFRYPIIGLFQLGGLSLLGLPNDLELVWGGPGGGSVVFMNVSSIANLYYFNGNTLTIVPNAYSIGFDTAESAYGVKVYSTFPSVFSPIVIETSGVNVPSVLWPIPPHVLVNQTSNKITVKLSISNKSLSGQAVYLETGFPPSVISSAVTNSSGIAVFPNNNYSFYVVYFPGNFTLSSTYYFSSPILNSLSSKFRSYYQDLLNFLNSAQNSFKKGIKSVLSKQETSITTTTLTSTTSSSSQFGVNLYIVLYILAFVIGMVISAILIRFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WR7</accession>
    <name>Q97WR7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90372">
        <property type="entry name" value="D90372"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WR7"/>
    <dbReference type="PaxDb" id="273057-SSO2050"/>
    <dbReference type="EnsemblBacteria" id="AAK42235">
        <property type="protein sequence ID" value="AAK42235"/>
        <property type="gene ID" value="SSO2050"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2050"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2130"/>
    <dbReference type="eggNOG" id="arCOG08427">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1607235_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WR7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="171" mass="19102" checksum="42ECA5C0635AB807" modified="2001-10-01" version="1">MKSVEIVMKFSFLLLLIITVISKTFMLGNYIIHVNVNENFPLVQSVENSTIGIKFVVSVKNFTVEPQTIDKLLAIKNSTNFSEIIVPYYLINKTFSIVYNGSYYANITISSVQVNDSNNFGGNLTNVSFISTSSSPLALNTFYSNGVIVMVLVATFSIVLYTILQTKKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WT0</accession>
    <name>Q97WT0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ATP-dependent RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO2036</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90370">
        <property type="entry name" value="G90370"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923742.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WT0"/>
    <dbReference type="FunCoup" id="Q97WT0">
        <property type="interactions" value="113"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2036"/>
    <dbReference type="PaxDb" id="273057-SSO2036"/>
    <dbReference type="EnsemblBacteria" id="AAK42222">
        <property type="protein sequence ID" value="AAK42222"/>
        <property type="gene ID" value="SSO2036"/>
    </dbReference>
    <dbReference type="GeneID" id="84061163"/>
    <dbReference type="KEGG" id="sso:SSO2036"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2117"/>
    <dbReference type="eggNOG" id="arCOG00558">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_3_2"/>
    <dbReference type="InParanoid" id="Q97WT0"/>
    <dbReference type="PhylomeDB" id="Q97WT0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="9">
        <location>
            <begin position="15"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="46"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="229"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="15"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="360" mass="40883" checksum="215828E877108071" modified="2001-10-01" version="1">MIRISIALLDHQEQLPNMFENLSEDLRKALNEAGYIKPTRVQEVVIPELMNGKSVIVQAKTGSGKTAAYVIPILERNSTALILSPTRELATQILDEIKKLGKYKQIDVSLIIGGMSYDDQRQSKIVVGTPGRLLDLWSKGKIDFSGYDFVIVDEADRMLDMGFIDDIRMILNHCNAKIAGFFSATIPDEIMTLAKEFSKDLREIVLDEYKPVEEVKQKFVKVRNDWNDKVSKLLEEINGEKILVFARTRDRARKLYYLLKGKGVNVGLLSGDMPQSVRLKNFYGFKKGKYNVLVATDLASRGIDIIDVNKVINFDIPRDVETYIHRVGRTGRMGRVGQAITFYTFREADMIKRINNLLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WU9</accession>
    <name>Q97WU9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0033 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO9500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfur carrier protein TusA family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90368">
        <property type="entry name" value="B90368"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989047.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WU9"/>
    <dbReference type="FunCoup" id="Q97WU9">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO9500"/>
    <dbReference type="PaxDb" id="273057-SSO9500"/>
    <dbReference type="EnsemblBacteria" id="AAK42201">
        <property type="protein sequence ID" value="AAK42201"/>
        <property type="gene ID" value="SSO9500"/>
    </dbReference>
    <dbReference type="GeneID" id="15297473"/>
    <dbReference type="KEGG" id="sso:SSO9500"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2091"/>
    <dbReference type="eggNOG" id="arCOG02062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165255_1_2_2"/>
    <dbReference type="InParanoid" id="Q97WU9"/>
    <dbReference type="PhylomeDB" id="Q97WU9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00291">
        <property type="entry name" value="SirA_YedF_YeeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="entry name" value="TusA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279:SF6">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SirA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="2">
        <location>
            <begin position="11"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="80" mass="8801" checksum="3464139D4731EB57" modified="2001-10-01" version="1">MSQEVRIAKTLDARGMYCPGPVLETAKAIKQINVGEVLEILATDPAAKPDIEAWARRTGNQVVDIQQQGGVTRILIKRMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WW6</accession>
    <name>Q97WW6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">First ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1995</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90366">
        <property type="entry name" value="B90366"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WW6"/>
    <dbReference type="PaxDb" id="273057-SSO1995"/>
    <dbReference type="EnsemblBacteria" id="AAK42185">
        <property type="protein sequence ID" value="AAK42185"/>
        <property type="gene ID" value="SSO1995"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1995"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2071"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074024_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WW6"/>
    <dbReference type="PhylomeDB" id="Q97WW6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025471">
        <property type="entry name" value="DUF4322"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033541">
        <property type="entry name" value="transpos_ISH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14210">
        <property type="entry name" value="DUF4322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4322" evidence="3">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="123"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="290" mass="33627" checksum="28C059892E26BEF7" modified="2001-10-01" version="1">MLNFQGEKVEKVAKTLIPACLWNNSVENKSRAYDVSPQTVRNYVEKQGMEVIEKLLERARKISLKVLKGVKEIDLSIDWTTKTWYGKPVKGSSEKGNSWNYATTKYKGKVLLLAFIPQVNGMTKDEIVKVLVEQVMAMGFKIRLITLDAGFYTVDVLNFISQFKYIVAVPVGDVKVYEEFDGDYKTNSKRHRRDEQVKFRLLVYSKGKVRRKSLVYFARATNLDLSKREVLDLYNKVRGPIETSYRNIKAFLPFTGSTKFVFRTLIFVLALVLYIPYSRGRWGERSLDYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WY8</accession>
    <name>Q97WY8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1971</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90363">
        <property type="entry name" value="D90363"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WY8"/>
    <dbReference type="STRING" id="273057.SSO1971"/>
    <dbReference type="PaxDb" id="273057-SSO1971"/>
    <dbReference type="EnsemblBacteria" id="AAK42163">
        <property type="protein sequence ID" value="AAK42163"/>
        <property type="gene ID" value="SSO1971"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1971"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2048"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113598_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WY8"/>
    <dbReference type="PhylomeDB" id="Q97WY8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="1">
        <location>
            <begin position="6"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="170" mass="19217" checksum="D67B01DEDCF23C7A" modified="2001-10-01" version="1">MEAPVAGIDVSKDKLIMYFQGKLYEFTNDRQGFEEIREILPKGCKVGIESTGVYHVNLAKYLMGEYDVRIINPFILKKFKDFRGKKSDKNDAKKLAEIVVSMGSGFVTSDARELTSQWDFVIRSIARVKNRLRRDLVLLGYRDSLSKKNLEEVLRGGDSIVLAEVSFGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X42</accession>
    <name>Q97X42_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Extradiol ring-cleavage dioxygenase class III enzyme subunit B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1913</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DODA-type extradiol aromatic ring-opening dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90356">
        <property type="entry name" value="C90356"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X42"/>
    <dbReference type="STRING" id="273057.SSO1913"/>
    <dbReference type="PaxDb" id="273057-SSO1913"/>
    <dbReference type="EnsemblBacteria" id="AAK42106">
        <property type="protein sequence ID" value="AAK42106"/>
        <property type="gene ID" value="SSO1913"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1913"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1975"/>
    <dbReference type="eggNOG" id="arCOG01730">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046582_2_1_2"/>
    <dbReference type="InParanoid" id="Q97X42"/>
    <dbReference type="PhylomeDB" id="Q97X42"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07363">
        <property type="entry name" value="45_DOPA_Dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014436">
        <property type="entry name" value="Extradiol_dOase_DODA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004183">
        <property type="entry name" value="Xdiol_dOase_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30096">
        <property type="entry name" value="4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30096:SF0">
        <property type="entry name" value="4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02900">
        <property type="entry name" value="LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006157">
        <property type="entry name" value="Doxgns_DODA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53213">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Extradiol ring-cleavage dioxygenase class III enzyme subunit B" evidence="6">
        <location>
            <begin position="7"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="259" mass="29935" checksum="42C16F6FB43A0429" modified="2001-10-01" version="1">MMMTVGLFISHGSPTILTEENKWKDLLRNIGKDVMEKYKPDTIIVSSPHFISWSGDYYIENSEKLECIQDYYGFPEETYKYCYEAYNDVELVNEIVMASDGIIKEDNRWGLDHGAWIPLLFMFPEYKPKVVTISITDNSPESHYAIGEKIRNAVEKLGRKVLFLATGSPTHRLDLFYFKITPKPTNFDIILMDMIKSGEFDKILRINELYPKEYQTAMPEGNLNTLYILLGFVKPKRAEILGYDTPWPGVSMLAASFYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X47</accession>
    <name>Q97X47_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Multidrug resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1909</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90355">
        <property type="entry name" value="F90355"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988999.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X47"/>
    <dbReference type="FunCoup" id="Q97X47">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1909"/>
    <dbReference type="PaxDb" id="273057-SSO1909"/>
    <dbReference type="EnsemblBacteria" id="AAK42101">
        <property type="protein sequence ID" value="AAK42101"/>
        <property type="gene ID" value="SSO1909"/>
    </dbReference>
    <dbReference type="GeneID" id="1453428"/>
    <dbReference type="KEGG" id="sso:SSO1909"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1970"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_34_0_2"/>
    <dbReference type="InParanoid" id="Q97X47"/>
    <dbReference type="PhylomeDB" id="Q97X47"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17504">
        <property type="entry name" value="MFS_MMR_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000025">
        <property type="entry name" value="Major facilitator superfamily MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="382"/>
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        </location>
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            <begin position="9"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="472" mass="51620" checksum="D54D35E3594F40FE" modified="2001-10-01" version="1">MDPKGTMRTLITLTLMLTLVNYVETMVIPALPKIEDQFSTTATTVAWVTSAYLIVGAIASPLFGKLGDRYGKKKVYLLSIGFYSLAVLLAGFSTNIYFLIFARAIQGLGYATFPLAIAIITDLFPKERVAWAQGILSATLAAGPALGLLVGSYIVQDLGWPYAFHTAFILSIILVIVSAKYIVEIPQKTKEKIDYLGATFLMLTVVPLLVYLSNGPNVGWTSTSQFLLIVISIIAFPIFLWVEGRTNEPLMRLDLFRIRNIMVANIAGLISGAGMFLMFTGLVYYLQLPKPYGLGLSIIESGLLMAPVALVMMIVGPIVGRLINNIGPKPLLMIGSIISIVGYLLLDTFRYSIYEVVVDVMITAVGLVNIMIPLVNMVAVSLPEEQRGIGIGMNTLIRTIGSSIGPVISTVFMDTYTTWIIYDFNNQIVPVAQVPSYSAFHYMYTAAVGIMFLNLIVALFTRNYVIKAGQHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X76</accession>
    <name>Q97X76_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1059 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="ordered locus">SSO1877</name>
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        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
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        <lineage>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="Pfam" id="PF06348">
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    </dbReference>
    <proteinExistence type="predicted"/>
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            <dbReference type="EMBL" id="AAK42066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13227" checksum="5E0F5E7A83E24D69" modified="2001-10-01" version="1">MPKYTFKCEDVGMDCGFEIKNAGTEDELLEMLKIHAKSSHGVTSIPPDLLNKIKQNIKKVGKYYFSCASVGMNCGFEIMGAQSEQELLDELAVHAKMSHGMSSIPQDTLNKIKQNIKVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X86</accession>
    <name>Q97X86_SACS2</name>
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        <recommendedName>
            <fullName evidence="3">DUF4242 domain-containing protein</fullName>
        </recommendedName>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
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                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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            </authorList>
            <dbReference type="PubMed" id="11427726"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="PATRIC" id="fig|273057.12.peg.1919"/>
    <dbReference type="eggNOG" id="arCOG05360">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2419886_0_0_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
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            <dbReference type="EMBL" id="AAK42056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="10614" checksum="E56FB535B6154479" modified="2001-10-01" version="1">MKVVVVHQWEDSQKEIVTNFFNQLVSMAKGKKLPKGMKLENIKISQDAKTAICEWDVENMDLLMQAAKQFNISWKITPLVPQTLYEHKSFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XC3</accession>
    <name>Q97XC3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1819</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42018.1"/>
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    </dbReference>
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        <property type="entry name" value="C90345"/>
    </dbReference>
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    <dbReference type="PaxDb" id="273057-SSO1819"/>
    <dbReference type="EnsemblBacteria" id="AAK42018">
        <property type="protein sequence ID" value="AAK42018"/>
        <property type="gene ID" value="SSO1819"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1819"/>
    <dbReference type="HOGENOM" id="CLU_2243980_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XC3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="104" mass="11738" checksum="CF705A50FC35792F" modified="2001-10-01" version="1">MFITDFNSPSFISSSLTLLTRSSTLPLSISLYTLLSRARAFIKISKGELPAWSGCKRSEYNLVLGEISLILLMSVWESHFSDINDNICKIEPHQFYSNIAAYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XD9</accession>
    <name>Q97XD9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-grasp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1806</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90343">
        <property type="entry name" value="C90343"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923602.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XD9"/>
    <dbReference type="FunCoup" id="Q97XD9">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1806"/>
    <dbReference type="PaxDb" id="273057-SSO1806"/>
    <dbReference type="EnsemblBacteria" id="AAK42002">
        <property type="protein sequence ID" value="AAK42002"/>
        <property type="gene ID" value="SSO1806"/>
    </dbReference>
    <dbReference type="GeneID" id="1454781"/>
    <dbReference type="KEGG" id="sso:SSO1806"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1864"/>
    <dbReference type="eggNOG" id="arCOG01340">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007415_3_1_2"/>
    <dbReference type="InParanoid" id="Q97XD9"/>
    <dbReference type="PhylomeDB" id="Q97XD9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051538">
        <property type="entry name" value="Acyl-CoA_Synth/Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043938">
        <property type="entry name" value="Ligase_CoA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334:SF2">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13549">
        <property type="entry name" value="ATP-grasp_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19045">
        <property type="entry name" value="Ligase_CoA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="5">
        <location>
            <begin position="468"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="660" mass="72695" checksum="C5A4142375A3470F" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XI0</accession>
    <name>Q97XI0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Orf in partial transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1760</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90337">
        <property type="entry name" value="C90337"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989066.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XI0"/>
    <dbReference type="STRING" id="273057.SSO1760"/>
    <dbReference type="PaxDb" id="273057-SSO1760"/>
    <dbReference type="EnsemblBacteria" id="AAK41954">
        <property type="protein sequence ID" value="AAK41954"/>
        <property type="gene ID" value="SSO1760"/>
    </dbReference>
    <dbReference type="GeneID" id="1454733"/>
    <dbReference type="KEGG" id="sso:SSO1760"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1808"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="InParanoid" id="Q97XI0"/>
    <dbReference type="PhylomeDB" id="Q97XI0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04762">
        <property type="entry name" value="HTH_MerR-trunc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1660.10:FF:000013">
        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2170:FF:000002">
        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1390:FF:000002">
        <property type="entry name" value="ORF1 in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="4">
        <location>
            <begin position="55"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="194" mass="22664" checksum="48F28D8DFB2F35C8" modified="2001-10-01" version="1">MLRPKEACQRLGISYATLREYVKKGYIKPVILQSGKWRFREEDVEKLMGIVKRRKVVLYARVSSSTQKDDLVNQVKYLEEQVKEYDQVITDIGSGLNMKRKGFLKLLRMILNNEVSRVVIAYPDRLVRFGFEILEEVCKSHNCEIVVINQEDKTPEEELVEDLVSILVLFSGKLYGMRSHEYEKVKKCVEELKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XN8</accession>
    <name>Q97XN8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1675</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90328">
        <property type="entry name" value="F90328"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989130.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XN8"/>
    <dbReference type="STRING" id="273057.SSO1675"/>
    <dbReference type="PaxDb" id="273057-SSO1675"/>
    <dbReference type="EnsemblBacteria" id="AAK41885">
        <property type="protein sequence ID" value="AAK41885"/>
        <property type="gene ID" value="SSO1675"/>
    </dbReference>
    <dbReference type="GeneID" id="1454664"/>
    <dbReference type="KEGG" id="sso:SSO1675"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1722"/>
    <dbReference type="eggNOG" id="arCOG04039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_701359_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XN8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="393" mass="45548" checksum="C34C6B6DEE606E03" modified="2001-10-01" version="1">MGTECNYNQPERTSIEGWKKYVSNCLVPVYEKLKLLQKVGEYLEQNTLNRSLDKIDPQLLPYFLGGVNENGDYIKGSLARLITYMYGVGVDTKKLANSINIGVKNPYSEAYVGKVNNLVIGFFTFTDVLNATAKQLELLFNKLNVSINSPSFAEIESNARNVDKFIQTVNDYIYRAKLILPMYNYYSFLIVTTRHLPASIFLDSYPEEFTKNPNLGYDFLGIGILHIPYISDKEQSARLGLTGHNRNTVSDLIFKIINNNLFMINKLYDVIENREIYDREHFTEEYYQFMTDVLRKSSIIVDASNLRYNYYMEIHYYQNLSVRICYASSPSYCEYGRTDRATFKYYSTISDMYNDPEIISILFMNGYININIKEDAGLNIYFRGQFGTEVILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XT7</accession>
    <name>Q97XT7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90322">
        <property type="entry name" value="D90322"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923522.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XT7"/>
    <dbReference type="STRING" id="273057.SSO1620"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1620"/>
    <dbReference type="EnsemblBacteria" id="AAK41835">
        <property type="protein sequence ID" value="AAK41835"/>
        <property type="gene ID" value="SSO1620"/>
    </dbReference>
    <dbReference type="GeneID" id="1454614"/>
    <dbReference type="KEGG" id="sso:SSO1620"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1662"/>
    <dbReference type="eggNOG" id="arCOG01417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053640_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XT7"/>
    <dbReference type="PhylomeDB" id="Q97XT7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="220"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="405" mass="46739" checksum="B02ABA452FCB76A2" modified="2001-10-01" version="1">MKITVVTSGLRSNYSGGSVHVNNVVRRLTKYFEVEFIPSISFFINNDRINEEIENIKKLGIKIPDFISFLSKFSNLKPSFLPYSLSVYNEYAERVKKVDSDFIYDPDYTTPESIFISNKVGIPLGLTLHEPLYSFSQSIFYTYYTLRPFFLQSLDYFLKRSIGLYLLTRSKANYISKSRYLSFIAAVSKGTLDSVKIKNSEKYILYPGNGVDKELLQYRTTKKEDYVIFWTTLIPPKGILNFLYIINLLKKRGISVRAKVSGKFIYDKFKRLFFNYIRDNGLDVEYLGFLDRKELYVTVSKAKLLIYPSLADGFSITILESLALGTPVIAYSIPTVHSVFKDIPAVKFVKEGDIRKFVDEVENELKTGNLQEAIYDKRVNSFIEFHNWDNVAENVAKIIKCSLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XV6</accession>
    <name>Q97XV6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1602</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90319">
        <property type="entry name" value="F90319"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XV6"/>
    <dbReference type="STRING" id="273057.SSO1602"/>
    <dbReference type="PaxDb" id="273057-SSO1602"/>
    <dbReference type="EnsemblBacteria" id="AAK41813">
        <property type="protein sequence ID" value="AAK41813"/>
        <property type="gene ID" value="SSO1602"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1602"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1640"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170813_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XV6"/>
    <dbReference type="PhylomeDB" id="Q97XV6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="113" mass="13671" checksum="85863ADCAF8A583C" modified="2001-10-01" version="1">MRRYENNLKGYEEILKMKPCTIVLEPTGVYAIRSSQYFKEKGIKVLQVSPNMLSREKEFRGKKTDFYDAEKLENMVDKAKEYEYNPLRELVTLYLFLNDIEVNYRNRLKRHYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XX0</accession>
    <name>Q97XX0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH gntR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1589</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90317">
        <property type="entry name" value="H90317"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923489.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XX0"/>
    <dbReference type="STRING" id="273057.SSO1589"/>
    <dbReference type="PaxDb" id="273057-SSO1589"/>
    <dbReference type="EnsemblBacteria" id="AAK41799">
        <property type="protein sequence ID" value="AAK41799"/>
        <property type="gene ID" value="SSO1589"/>
    </dbReference>
    <dbReference type="GeneID" id="84061013"/>
    <dbReference type="KEGG" id="sso:SSO1589"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1624"/>
    <dbReference type="eggNOG" id="arCOG04027">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_10_2_2"/>
    <dbReference type="InParanoid" id="Q97XX0"/>
    <dbReference type="PhylomeDB" id="Q97XX0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38445">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REPRESSOR YTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38445:SF9">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REPRESSOR YTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="14"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="117" mass="13411" checksum="2A3BD997671EFEE9" modified="2001-10-01" version="1">MTNITIRIDLNSKKQIYEQIANQIIELIAKGVLNPGDPLPSVREMAAMLSVNMLTVEKAYKYLEGEGFVTVYKKRFVVKTDIRDEKWKELMKEAVYRALAGGAKRDEILFFISALLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XY5</accession>
    <name>Q97XY5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cupin type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1570</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="H90315"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993255.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XY5"/>
    <dbReference type="STRING" id="273057.SSO1570"/>
    <dbReference type="PaxDb" id="273057-SSO1570"/>
    <dbReference type="EnsemblBacteria" id="AAK41783">
        <property type="protein sequence ID" value="AAK41783"/>
        <property type="gene ID" value="SSO1570"/>
    </dbReference>
    <dbReference type="GeneID" id="1454562"/>
    <dbReference type="KEGG" id="sso:SSO1570"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1606"/>
    <dbReference type="eggNOG" id="arCOG02994">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116722_4_2_2"/>
    <dbReference type="InParanoid" id="Q97XY5"/>
    <dbReference type="PhylomeDB" id="Q97XY5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051610">
        <property type="entry name" value="GPI/OXD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35848:SF6">
        <property type="entry name" value="CUPIN TYPE-2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35848">
        <property type="entry name" value="OXALATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="2">
        <location>
            <begin position="44"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="127" mass="14710" checksum="C358B210A953262F" modified="2001-10-01" version="1">MVDYLVSNVKEVKMDRVPVNGSKNAFIQWLVTKENGANYAVRKFTLLKDGIIPMHIHKYQETVIVTKGKCKVCVSDKVYELREGDYIFINSNVKHAIINEGDQLEFFCIIDYSDDMRIITLDEDCNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y11</accession>
    <name>Q97Y11_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1539</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90313">
        <property type="entry name" value="A90313"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923459.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y11"/>
    <dbReference type="FunCoup" id="Q97Y11">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1539"/>
    <dbReference type="PaxDb" id="273057-SSO1539"/>
    <dbReference type="EnsemblBacteria" id="AAK41760">
        <property type="protein sequence ID" value="AAK41760"/>
        <property type="gene ID" value="SSO1539"/>
    </dbReference>
    <dbReference type="GeneID" id="1454539"/>
    <dbReference type="KEGG" id="sso:SSO1539"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1577"/>
    <dbReference type="eggNOG" id="arCOG02691">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_0_2"/>
    <dbReference type="InParanoid" id="Q97Y11"/>
    <dbReference type="PhylomeDB" id="Q97Y11"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051084">
        <property type="entry name" value="H+-coupled_symporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43528">
        <property type="entry name" value="ALPHA-KETOGLUTARATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43528:SF1">
        <property type="entry name" value="ALPHA-KETOGLUTARATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0769">Symport</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="332"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="12"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="430" mass="46502" checksum="C64D5B917C9318A5" modified="2001-10-01" version="1">MVYGLNKQQWLAVFSTWLGWLMDGYTSIAYALVAVTISKIFFPSTIGILGLIATFGGFAVGALARPVGSLVFGNFIGDKIGRKNMLVLTILGFSLIASSKALLPSYETAGILAPLFLYIILFAEGMFAGAEYGGGTTLALESVPVGKRGFIGSFVQSGFGTGYFVISLVYSALYSMFGNEGFQTLGWRVLFATCILPGLITLIIRKMTDESPIFKDMKSGNEVVKIPIKELFKMSYSSVLIGLMITSGLLYINTATFSFYPTVLTIQGIPGTIVGLSVAIINLVSLFGVWFGGFLADVIKRGRKVPMLIYSIIFIFTVYPVLYLGLLKNVYLSTIVFSLQAFLEAMIFSTLPAFLAEQFSKKYRTTGVGFTYNGGAIGGGFAISATLALSTYLGLLYSWSINIIIAGIIMIMGIVLAKETYTGKEDPILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y49</accession>
    <name>Q97Y49_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">First ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1497</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="C90308"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y49"/>
    <dbReference type="STRING" id="273057.SSO1497"/>
    <dbReference type="PaxDb" id="273057-SSO1497"/>
    <dbReference type="DNASU" id="1454501"/>
    <dbReference type="EnsemblBacteria" id="AAK41722">
        <property type="protein sequence ID" value="AAK41722"/>
        <property type="gene ID" value="SSO1497"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1497"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1540"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2152736_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y49"/>
    <dbReference type="PhylomeDB" id="Q97Y49"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1390:FF:000002">
        <property type="entry name" value="ORF1 in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="2">
        <location>
            <begin position="8"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="111" mass="12749" checksum="9AA2F1986499DE22" modified="2001-10-01" version="1">MGIIRKRKVILYARVPSSTQKDELVNQVKYLEEQVKEYDQVISDIGSGLNMKRKGFLKLLRMILNNEVSRVVVAYPDRLVRLRNLRGSVQSTQLRNSSTKPEGQNTRTGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y82</accession>
    <name>Q97Y82_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA2/NAM7 helicase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90303">
        <property type="entry name" value="D90303"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y82"/>
    <dbReference type="SMR" id="Q97Y82"/>
    <dbReference type="STRING" id="273057.SSO1456"/>
    <dbReference type="PaxDb" id="273057-SSO1456"/>
    <dbReference type="EnsemblBacteria" id="AAK41683">
        <property type="protein sequence ID" value="AAK41683"/>
        <property type="gene ID" value="SSO1456"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1456"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1486"/>
    <dbReference type="eggNOG" id="arCOG00792">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1840678_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y82"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF495">
        <property type="entry name" value="HELICASE SENATAXIN ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="1">
        <location>
            <begin position="32"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="139" mass="15907" checksum="208D7502FDD506C7" modified="2001-10-01" version="1">MRLEIAEKYMAEAYEYLKKGDTIQASGKAYKVAEEIVKAIKFLISKLIGQDVGVISPYRTQVRKLDQELANYKPYVEVNTVDAFQGREKDVIIFSVTATNGLRFVTNRRRLNVAFTRPRYKLIVLGNENSIMSCQMTIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YA6</accession>
    <name>Q97YA6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR type III-associated protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1426</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2023" name="Curr. Res. Struct. Biol." volume="5" first="100098" last="100098">
            <title>Structure of the &lt;i&gt;Saccharolobus solfataricus&lt;/i&gt; type III-D CRISPR effector.</title>
            <authorList>
                <person name="Cannone G."/>
                <person name="Kompaniiets D."/>
                <person name="Graham S."/>
                <person name="White M.F."/>
                <person name="Spagnolo L."/>
            </authorList>
            <dbReference type="PubMed" id="36843655"/>
            <dbReference type="DOI" id="10.1016/j.crstbi.2023.100098"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (3.50 ANGSTROMS) OF 4-281</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90300">
        <property type="entry name" value="E90300"/>
    </dbReference>
    <dbReference type="PDB" id="8BMW">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="H/I=4-281"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YA6"/>
    <dbReference type="SMR" id="Q97YA6"/>
    <dbReference type="STRING" id="273057.SSO1426"/>
    <dbReference type="PaxDb" id="273057-SSO1426"/>
    <dbReference type="EnsemblBacteria" id="AAK41660">
        <property type="protein sequence ID" value="AAK41660"/>
        <property type="gene ID" value="SSO1426"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1426"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1452"/>
    <dbReference type="eggNOG" id="arCOG02658">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084433_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YA6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013411">
        <property type="entry name" value="CRISPR-assoc_RAMP_Csx7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052216">
        <property type="entry name" value="CRISPR_Csm3_endoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02581">
        <property type="entry name" value="cas_cyan_RAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35579">
        <property type="entry name" value="CRISPR SYSTEM CMS ENDORIBONUCLEASE CSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35579:SF6">
        <property type="entry name" value="DUF324 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="5" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="85"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="93"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="8BMW"/>
        </source>
    </evidence>
    <sequence length="281" mass="31527" checksum="D2AD7A1B56AB65AE" modified="2001-10-01" version="1">MVLMVNYTFIDKRVIKRTTMIEGDVETVSPLKIGGGKDNFDPSSLAKDSILKDVEGRPIIPGSSWKGIFRSTGERILRLRNIEVCSGIGKDYCLNNNRKERDFNSALKENVDQALEIFWDYTCLNCKVFGTMSVIGAVRFLDSLPISYSLNTRSMIAISRTEGAVARRALVTVEYVDVGSKFSFKMMGYNLPNYAIGYLITIMKNIHDGFTQVGGHKSRGFGFVKFGKVKFTDLGEKRIGDEDIQVKDVGDLVEGNGDEFFGRMKPFMEAFNNAKIPYPKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YC6</accession>
    <name>CAS5B_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated protein Cas5 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cas5b</name>
        <name type="ordered locus">SSO1400</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="J. Biol. Chem." volume="286" first="21643" last="21656">
            <title>Structural and functional characterization of an archaeal clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for antiviral defense (CASCADE).</title>
            <authorList>
                <person name="Lintner N.G."/>
                <person name="Kerou M."/>
                <person name="Brumfield S.K."/>
                <person name="Graham S."/>
                <person name="Liu H."/>
                <person name="Naismith J.H."/>
                <person name="Sdano M."/>
                <person name="Peng N."/>
                <person name="She Q."/>
                <person name="Copie V."/>
                <person name="Young M.J."/>
                <person name="White M.F."/>
                <person name="Lawrence C.M."/>
            </authorList>
            <dbReference type="PubMed" id="21507944"/>
            <dbReference type="DOI" id="10.1074/jbc.M111.238485"/>
        </citation>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35091 / DSM 1616 / JCM 8930 / NBRC 15331 / P1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the aCascade ribonucleoprotein complex, minimally composed of Csa2 and Cas5a, which binds crRNA. Other possible components of aCascade in strain P1 are Cas6b (SSO1437) and Csa5 (SSO1443), while SSO1399, Cas5b (SSO1400) and SSO1401 have sometimes been seen weakly associated. Csa2 is probably the major RNA-binding subunit. The Csa2-Cas5a-crRNA complex also binds target DNA homologous to crRNA, probably forming an R-loop. Purified aCascade forms a filament about 6 nm in width.</text>
    </comment>
    <comment type="miscellaneous">
        <text>The aCascade complex was purified from strain P1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR-associated protein Cas5 family. Subtype I-A/Apern subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90297">
        <property type="entry name" value="D90297"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988394.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YC6"/>
    <dbReference type="SMR" id="Q97YC6"/>
    <dbReference type="STRING" id="273057.SSO1400"/>
    <dbReference type="PaxDb" id="273057-SSO1400"/>
    <dbReference type="EnsemblBacteria" id="AAK41635">
        <property type="protein sequence ID" value="AAK41635"/>
        <property type="gene ID" value="SSO1400"/>
    </dbReference>
    <dbReference type="GeneID" id="1454414"/>
    <dbReference type="KEGG" id="sso:SSO1400"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1416"/>
    <dbReference type="eggNOG" id="arCOG02670">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1072073_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YC6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09753">
        <property type="entry name" value="Cas5_I-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013422">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010153">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5a-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053725">
        <property type="entry name" value="CRISPR_Cas5_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01874">
        <property type="entry name" value="cas_cas5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02593">
        <property type="entry name" value="CRISPR_cas5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000417893" description="CRISPR-associated protein Cas5 2">
        <location>
            <begin position="1"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="21507944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="263" mass="29956" checksum="A5BBE740FACE34CC" modified="2001-10-01" version="1">MKGVVIFGIHHWGFSVRVPRTSAGGSSYIVPPITTILGALSRGYCSDYAVKNNVSCTKEFIDKFSSDLFWVTYGTEEHMLMPYSDLLREERVPYRQSKNRSLDHVSDWFGVSAFGKVYCENVNFSILLLLNKENSEFWSKLGWQIISLGTKESLVTITDVKVVDVEESSDEDIYTIYYIPAECLTNTEEFEVVNMPVKSVYELSSKPSIGVFSNFLVPRKTPFIGGKVKVKKSNVRGDICKVYKMADKYVITFKEGLEKWYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YD8</accession>
    <name>Q97YD8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First part of one of two inversely orientated ORFs in a partial ISC1043</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO7898</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90295">
        <property type="entry name" value="H90295"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YD8"/>
    <dbReference type="STRING" id="273057.SSO7898"/>
    <dbReference type="PaxDb" id="273057-SSO7898"/>
    <dbReference type="EnsemblBacteria" id="AAK41623">
        <property type="protein sequence ID" value="AAK41623"/>
        <property type="gene ID" value="SSO7898"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7898"/>
    <dbReference type="HOGENOM" id="CLU_2550446_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YD8"/>
    <dbReference type="PhylomeDB" id="Q97YD8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="98" mass="11457" checksum="E0FB5A297489B879" modified="2001-10-01" version="1">MNPLFPNLIYDPPCPPFNEDQRLSPLTLEILLQILQWVPHEPQYHLPPPFPSLDRGVRLRYQVDLRIREVIQCRLVVLISSQESTRTARSLRTSRAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YF6</accession>
    <name>Q97YF6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="4">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pdhA-1</name>
        <name evidence="9" type="ordered locus">SSO1369</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90293">
        <property type="entry name" value="D90293"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992547.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YF6"/>
    <dbReference type="FunCoup" id="Q97YF6">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1369"/>
    <dbReference type="PaxDb" id="273057-SSO1369"/>
    <dbReference type="EnsemblBacteria" id="AAK41603">
        <property type="protein sequence ID" value="AAK41603"/>
        <property type="gene ID" value="SSO1369"/>
    </dbReference>
    <dbReference type="GeneID" id="1454382"/>
    <dbReference type="KEGG" id="sso:SSO1369"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1374"/>
    <dbReference type="eggNOG" id="arCOG01054">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029393_5_0_2"/>
    <dbReference type="InParanoid" id="Q97YF6"/>
    <dbReference type="PhylomeDB" id="Q97YF6"/>
    <dbReference type="BRENDA" id="2.3.1.190">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="8">
        <location>
            <begin position="57"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="345" mass="38371" checksum="9776A5A59941CE80" modified="2001-10-01" version="1">MIIKPEPSLKDLSYIIENAGLKASDLLNMYKRMLIIRYFEESIRKIYHEGKNPFNMASGRIRGEMHLSIGQEAVAVGTLYDIRDEDVVVSTHRPHHHAIAKGVDLKGLAAEILGKATGLCKGKGGHMHLFDKSKNFACSGIVGASFPQAAGAAFAFKYLGKDNVAISFAGEGAANHGTFAETLNIASAWELPLIIVIEDNKYADSTPKSFVMSTTFHYQRGLAYNVPSYLVDGMDVIDVYSTSKKAIERARKGFGPTLIEALTYRYVGHFEGDGEEYRTKEEVEFWSSLDPIRRLENRLLRLNYADSDILARLREEARKQVQEAIDFAINSKYPELTDAFGGVFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="138" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YP7</accession>
    <name>Q97YP7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligo/dipeptide transport, ATP binding protein (DppF-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dppF-1</name>
        <name evidence="6" type="ordered locus">SSO1277</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90282">
        <property type="entry name" value="C90282"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990037.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YP7"/>
    <dbReference type="FunCoup" id="Q97YP7">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1277"/>
    <dbReference type="PaxDb" id="273057-SSO1277"/>
    <dbReference type="EnsemblBacteria" id="AAK41514">
        <property type="protein sequence ID" value="AAK41514"/>
        <property type="gene ID" value="SSO1277"/>
    </dbReference>
    <dbReference type="GeneID" id="1454293"/>
    <dbReference type="KEGG" id="sso:SSO1277"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1274"/>
    <dbReference type="eggNOG" id="arCOG00184">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="InParanoid" id="Q97YP7"/>
    <dbReference type="PhylomeDB" id="Q97YP7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050319">
        <property type="entry name" value="ABC_transp_ATP-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776:SF7">
        <property type="entry name" value="D,D-DIPEPTIDE TRANSPORT ATP-BINDING PROTEIN DDPF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776">
        <property type="entry name" value="TRANSPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="15"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="325" mass="36913" checksum="1208EABC10002DA8" modified="2001-10-01" version="1">MVMRLPVGSTSNYVVKVENLKVYFTKGGMFTKKYVVKALDGVTLSIKEREIMGVVGESGSGKTTLGRVTIGLQKPTSGNVYIKADNKEINVNKAKLKDISNYVQMIYQDPYSSIDPIMRVYDVLATPLKYKKMSDIDKKIEEAMRLVDLPLELLENRVYQLSGGQRQRLSIARAIVVNPKYIVADEPTTMLDASLKGEILKIIKDARESKGFSFMYITHELPIAKIISDRIIVLYLGKIVELGNSNEIIKNPLHPYTQGLIEVYPRIDPSLRDKIKEIKIKVDIVRPDKGCVFYPRCPFAMPKCKEQEPELKEVSQNHYVACWLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YT3</accession>
    <name>Q97YT3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Heme-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1224</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90276">
        <property type="entry name" value="H90276"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923262.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YT3"/>
    <dbReference type="STRING" id="273057.SSO1224"/>
    <dbReference type="PaxDb" id="273057-SSO1224"/>
    <dbReference type="EnsemblBacteria" id="AAK41471">
        <property type="protein sequence ID" value="AAK41471"/>
        <property type="gene ID" value="SSO1224"/>
    </dbReference>
    <dbReference type="GeneID" id="1454250"/>
    <dbReference type="KEGG" id="sso:SSO1224"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1225"/>
    <dbReference type="eggNOG" id="arCOG07054">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103773_2_2_2"/>
    <dbReference type="InParanoid" id="Q97YT3"/>
    <dbReference type="PhylomeDB" id="Q97YT3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.150">
        <property type="entry name" value="Haem-degrading domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052517">
        <property type="entry name" value="GlcG_carb_metab_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005624">
        <property type="entry name" value="PduO/GlcC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038084">
        <property type="entry name" value="PduO/GlcC-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34309:SF1">
        <property type="entry name" value="PROTEIN GLCG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34309">
        <property type="entry name" value="SLR1406 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03928">
        <property type="entry name" value="HbpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143744">
        <property type="entry name" value="GlcG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15041" checksum="AED019143F4042AB" modified="2001-10-01" version="1">MNILNKLGISDELADMLINEAVRKAKELGVNVSIAIVDEGGELKAFKRMDGAPILTIQIAIDKSWTAVSFGIPTHEWYNILKDNPSLAMGFPKIPRLIIFGGGFPIIYKEQVIGGIGVSGASAEQDMEIAKSALKRLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YV6</accession>
    <name>Q97YV6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1197</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90273">
        <property type="entry name" value="F90273"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YV6"/>
    <dbReference type="STRING" id="273057.SSO1197"/>
    <dbReference type="PaxDb" id="273057-SSO1197"/>
    <dbReference type="EnsemblBacteria" id="AAK41445">
        <property type="protein sequence ID" value="AAK41445"/>
        <property type="gene ID" value="SSO1197"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1197"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1197"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113185_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YV6"/>
    <dbReference type="PhylomeDB" id="Q97YV6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="158" mass="17803" checksum="844EC409007DE144" modified="2001-10-01" version="1">MTKELTREEYYKALEKAVNEVILSMTGTRKDVAKRLVLGAVVGRNATEIAQEAEMDYETVLNNLDKAAQAKLIEVVKKLVGDHPVLLIVDDTHDHKLYARAMPVSRNGAQIFYCRAHKRFEPAIQLLVIGVKDLVNNQIYVSAFRKFIALGKGYITRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YZ1</accession>
    <name>Q97YZ1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Haloacetate dehalogenase</fullName>
            <ecNumber evidence="3">3.8.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily. S-2-haloalkanoic acid dehalogenase family.</text>
    </comment>
    <dbReference type="EC" id="3.8.1.3" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90269">
        <property type="entry name" value="B90269"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991585.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YZ1"/>
    <dbReference type="SMR" id="Q97YZ1"/>
    <dbReference type="STRING" id="273057.SSO1159"/>
    <dbReference type="PaxDb" id="273057-SSO1159"/>
    <dbReference type="EnsemblBacteria" id="AAK41409">
        <property type="protein sequence ID" value="AAK41409"/>
        <property type="gene ID" value="SSO1159"/>
    </dbReference>
    <dbReference type="GeneID" id="1454188"/>
    <dbReference type="KEGG" id="sso:SSO1159"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1152"/>
    <dbReference type="eggNOG" id="arCOG02291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_3_1_2"/>
    <dbReference type="InParanoid" id="Q97YZ1"/>
    <dbReference type="PhylomeDB" id="Q97YZ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018785">
        <property type="term" value="F:haloacetate dehalogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02588">
        <property type="entry name" value="HAD_L2-DEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006328">
        <property type="entry name" value="2-HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051540">
        <property type="entry name" value="S-2-haloacid_dehalogenase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01493">
        <property type="entry name" value="HAD-SF-IA-v2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01428">
        <property type="entry name" value="HAD_type_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316:SF3">
        <property type="entry name" value="HALOACID DEHALOGENASE, TYPE II (AFU_ORTHOLOGUE AFUA_2G07750)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43316">
        <property type="entry name" value="HYDROLASE, HALOACID DELAHOGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="198" mass="23010" checksum="DE52F43A827479D8" modified="2001-10-01" version="1">MGKEKKILAFDLYGTILDLSAISQEMRRKQLEYTWLLTIMGRYVDFDEITKIAISYTLGEDKIEEELNKWRSLRTHQDAIYLKDISTIADIYILSNGTTKTIEELLKLNGLSSYFKGIFSAERVKEYKPSPKVYRYFLEAVKGEAYLVSSNPFDVIGAKNSGMKGIYVNRRNMPVDPLGYEPDVVVRDFKELYEWLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z06</accession>
    <name>Q97Z06_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protease related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO1141</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90267">
        <property type="entry name" value="A90267"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923205.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z06"/>
    <dbReference type="SMR" id="Q97Z06"/>
    <dbReference type="STRING" id="273057.SSO1141"/>
    <dbReference type="PaxDb" id="273057-SSO1141"/>
    <dbReference type="EnsemblBacteria" id="AAK41392">
        <property type="protein sequence ID" value="AAK41392"/>
        <property type="gene ID" value="SSO1141"/>
    </dbReference>
    <dbReference type="GeneID" id="1454171"/>
    <dbReference type="KEGG" id="sso:SSO1141"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1132"/>
    <dbReference type="eggNOG" id="arCOG03665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006754_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z06"/>
    <dbReference type="PhylomeDB" id="Q97Z06"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000027">
        <property type="entry name" value="Peptidase S53 propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017001">
        <property type="entry name" value="Pept_S53_physarolisin-II_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF15">
        <property type="entry name" value="TRIPEPTIDYL-PEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17957">
        <property type="entry name" value="Big_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032623">
        <property type="entry name" value="Peptidase_SSO2181_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1245"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S53" evidence="9">
        <location>
            <begin position="196"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK41392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="1269" mass="138029" checksum="87C8F05CCF83DA84" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z23</accession>
    <name>Q97Z23_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90264">
        <property type="entry name" value="G90264"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989750.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z23"/>
    <dbReference type="STRING" id="273057.SSO1119"/>
    <dbReference type="PaxDb" id="273057-SSO1119"/>
    <dbReference type="EnsemblBacteria" id="AAK41374">
        <property type="protein sequence ID" value="AAK41374"/>
        <property type="gene ID" value="SSO1119"/>
    </dbReference>
    <dbReference type="GeneID" id="1454153"/>
    <dbReference type="KEGG" id="sso:SSO1119"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1114"/>
    <dbReference type="eggNOG" id="arCOG05895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_658272_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z23"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012440">
        <property type="entry name" value="DUF1641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017007">
        <property type="entry name" value="UCP032762"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07849">
        <property type="entry name" value="DUF1641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032762">
        <property type="entry name" value="UCP032762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="46461" checksum="4B980CE70418B830" modified="2001-10-01" version="1">MSVSTVDPLEEILKPENLSRLSKIVDALPTIEKVTDKITEMDKKGQVDFLLSLFDQTVSILDAVQKADLINTLISFGMDQLPKIQAIWPILEKLTSERALQLLSQIDIDSTLTALEKLSPIIKKLTDEKVLKVIDQIDYDSLIDSTSKLVPILSKLANERTVKTIEALDIDMLLNLASKMAPTLNKFASLMDQMSSKGQVDMLVNLMEQGISLLDAVQKADLINTLISFGMDQLPKIQAIWPILEKLTSERTLNLIQSLDLDSMFNALEALTPIMKQLTSDKAIKIIQQFDIASTLGALEAAMPLLKKLTDEKTVKIISQIDVNSLLMLTNKLVEMQKSGSLDRLMQLLEIISDPQFVNGLVTVMDKFSKAFKAWVNDIPNARPVGTMGLLRATSDKDVSYALGLMLELAKEVGKSFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z31</accession>
    <name>Q97Z31_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0033 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90263">
        <property type="entry name" value="G90263"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z31"/>
    <dbReference type="PaxDb" id="273057-SSO1109"/>
    <dbReference type="EnsemblBacteria" id="AAK41366">
        <property type="protein sequence ID" value="AAK41366"/>
        <property type="gene ID" value="SSO1109"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1109"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1106"/>
    <dbReference type="eggNOG" id="arCOG02072">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165242_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z31"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00291">
        <property type="entry name" value="SirA_YedF_YeeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="entry name" value="TusA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279:SF18">
        <property type="entry name" value="SULFUR CARRIER PROTEIN MJ0990-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SirA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="1">
        <location>
            <begin position="37"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="121" mass="13934" checksum="3AF7075DC83BFBFD" modified="2001-10-01" version="1">MCIQLQIAYCILGIRPSCCQNKIFYLYNVNSLTMIEEMDLTPLECPEPFMKVVAKLMKIENGELKIRYKDPKCREMLLEAMKLMNCKVLEDSQNNGVFFMHIKKESGSSEKPKKIELTGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z63</accession>
    <name>Q97Z63_SACS2</name>
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        <submittedName>
            <fullName evidence="6">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1069</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
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            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90259">
        <property type="entry name" value="E90259"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923185.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z63"/>
    <dbReference type="FunCoup" id="Q97Z63">
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    </dbReference>
    <dbReference type="STRING" id="273057.SSO1069"/>
    <dbReference type="PaxDb" id="273057-SSO1069"/>
    <dbReference type="EnsemblBacteria" id="AAK41332">
        <property type="protein sequence ID" value="AAK41332"/>
        <property type="gene ID" value="SSO1069"/>
    </dbReference>
    <dbReference type="GeneID" id="1454111"/>
    <dbReference type="KEGG" id="sso:SSO1069"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1065"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_16_0_2"/>
    <dbReference type="InParanoid" id="Q97Z63"/>
    <dbReference type="PhylomeDB" id="Q97Z63"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052962">
        <property type="entry name" value="AA_Transporter_AGT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547:SF1">
        <property type="entry name" value="ASPARTATE-PROTON SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="426"/>
            <end position="447"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="459"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="504"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="548"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="581"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="610"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="647" mass="69310" checksum="C4174F7215DCD4C4" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZA5</accession>
    <name>Q97ZA5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1025</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
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        <lineage>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="A99254"/>
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    <dbReference type="AlphaFoldDB" id="Q97ZA5"/>
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    <dbReference type="EnsemblBacteria" id="AAK41288">
        <property type="protein sequence ID" value="AAK41288"/>
        <property type="gene ID" value="SSO1025"/>
    </dbReference>
    <dbReference type="GeneID" id="15297572"/>
    <dbReference type="KEGG" id="sso:SSO1025"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1017"/>
    <dbReference type="eggNOG" id="arCOG03626">
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    <dbReference type="HOGENOM" id="CLU_088957_3_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="InterPro" id="IPR038730">
        <property type="entry name" value="HyfE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38601">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38601:SF1">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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            <end position="23"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="147"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23782" checksum="AAA989712EA0C70D" modified="2001-10-01" version="1">MINSEILELLSTFILISAFYIQGQAYFKPAIYAQAIQSTLIAILAFHLGISLFSIDYIILGITVILLRTVLITIFLFRGLVKEKPGARESSKGVASELILNLAFSIIASLVVYYFVLEKIPISTEINNTLMLLFAFVLLFQGLFLIISRKSTIFQFIGFIEEENSTILFGILLLPIPFLIEVSVFLDVLGLVIISSILTLEKAEHSRLDELKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZB1</accession>
    <name>Q97ZB1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sulfurtransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cysA-1</name>
        <name evidence="4" type="ordered locus">SSO1019</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90253">
        <property type="entry name" value="C90253"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZB1"/>
    <dbReference type="FunCoup" id="Q97ZB1">
        <property type="interactions" value="183"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1019"/>
    <dbReference type="PaxDb" id="273057-SSO1019"/>
    <dbReference type="EnsemblBacteria" id="AAK41282">
        <property type="protein sequence ID" value="AAK41282"/>
        <property type="gene ID" value="SSO1019"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1019"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1010"/>
    <dbReference type="eggNOG" id="arCOG02019">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031618_1_3_2"/>
    <dbReference type="InParanoid" id="Q97ZB1"/>
    <dbReference type="PhylomeDB" id="Q97ZB1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01449">
        <property type="entry name" value="TST_Repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051126">
        <property type="entry name" value="Thiosulfate_sulfurtransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43855">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43855:SF1">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00683">
        <property type="entry name" value="RHODANESE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="16"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="153"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="278" mass="32080" checksum="F091CAC2DF84B68C" modified="2001-10-01" version="1">MFFHIMLVSVEWLNNNLKDVKVVEIGYNPQINYYEGHIPGAVLINWRDFLDDNSRDFASPGKLSKVLGNAGINNDDLIVLYSEMNNRYAFYVYWILKAYGHSNLAILNGGIYKWLKEGYPIESDGIVVNKKSEYKARKPDWSSRILVWELLSKLKEFVLIDSRSKEEYDGLTTAPPEHKCEQTQMSGHIPGAKNIPWTILLNEDETMKSRDELERIFSWLSKEDKIVVYCRTGARASVAWYALKEVLGFKLVRLYDGSWVEYGNMVGVPVEKSVSDRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZE6</accession>
    <name>Q97ZE6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pus10 THUMP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0972</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90248">
        <property type="entry name" value="G90248"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992422.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZE6"/>
    <dbReference type="STRING" id="273057.SSO0972"/>
    <dbReference type="PaxDb" id="273057-SSO0972"/>
    <dbReference type="EnsemblBacteria" id="AAK41246">
        <property type="protein sequence ID" value="AAK41246"/>
        <property type="gene ID" value="SSO0972"/>
    </dbReference>
    <dbReference type="GeneID" id="1455213"/>
    <dbReference type="KEGG" id="sso:SSO0972"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.971"/>
    <dbReference type="eggNOG" id="arCOG01015">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_747264_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZE6"/>
    <dbReference type="PhylomeDB" id="Q97ZE6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055174">
        <property type="entry name" value="Pus10_THUMP_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011139">
        <property type="entry name" value="PRK14554.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22023">
        <property type="entry name" value="Pus10_THUMP_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pus10 THUMP" evidence="1">
        <location>
            <begin position="116"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="370" mass="42710" checksum="088BB7896B11421C" modified="2001-10-01" version="1">MINSSEGKSDNKIIEKATQILSKYPLCDSCLGRCFARLGYGLENKERGRAIKISLMLILDEKIKNHEIGDLSSIKRIMENLGPIAEKWYKLYFSSEFHSHPCYLCQNKIEDIKEDFSDKAFKLLSGLGVKSYVLGVELDEETKKKESKIIEEFTLMYYESIKHEIKREVGKTLSKRGYPPNMDNPEVEIVYRLSDLQVFIISKNIRTFYVYNRLNRNLPISSWFSKQGNEGLNTLLQRKIVFAFSEPTTVRILADYPIVIENEGRDKIDVGGYYIFKVMTVGKKELQAISAAKPTMRKYRVTVYSTSSLSDAIRVYGNIYDLYIDAKSFSELNEKLSKLKSQYEIIVLSVDLIDVKGRIKDIIENYLKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZE9</accession>
    <name>Q97ZE9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Type 2 DNA topoisomerase 6 subunit A</fullName>
            <ecNumber evidence="12">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Type II DNA topoisomerase VI subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 17" type="primary">top6A</name>
        <name evidence="17" type="ordered locus">SSO0969</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12 13">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11 12">Homodimer. Heterotetramer of two Top6A and two Top6B chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12 13">Belongs to the TOP6A family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="12"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90248">
        <property type="entry name" value="D90248"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992416.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZE9"/>
    <dbReference type="FunCoup" id="Q97ZE9">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0969"/>
    <dbReference type="PaxDb" id="273057-SSO0969"/>
    <dbReference type="EnsemblBacteria" id="AAK41243">
        <property type="protein sequence ID" value="AAK41243"/>
        <property type="gene ID" value="SSO0969"/>
    </dbReference>
    <dbReference type="GeneID" id="1455210"/>
    <dbReference type="KEGG" id="sso:SSO0969"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.968"/>
    <dbReference type="eggNOG" id="arCOG04143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037229_1_0_2"/>
    <dbReference type="InParanoid" id="Q97ZE9"/>
    <dbReference type="PhylomeDB" id="Q97ZE9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000655">
        <property type="entry name" value="Type 2 DNA topoisomerase 6 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1360.10:FF:000011">
        <property type="entry name" value="Type 2 DNA topoisomerase 6 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00132">
        <property type="entry name" value="Top6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049333">
        <property type="entry name" value="Topo_VI_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004085">
        <property type="entry name" value="TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003336">
        <property type="entry name" value="PRK04342.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848:SF0">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21180">
        <property type="entry name" value="TOP6A-Spo11_Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20768">
        <property type="entry name" value="Topo_VI_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01552">
        <property type="entry name" value="TPISMRASE6A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="DNA topoisomerase IV, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52041">
        <property type="entry name" value="TOPO_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 12 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12 13" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Spo11/DNA topoisomerase VI subunit A N-terminal" evidence="14">
        <location>
            <begin position="77"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Type II DNA topoisomerase VI subunit A all-beta" evidence="15">
        <location>
            <begin position="159"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Topoisomerase 6 subunit A/Spo11 TOPRIM" evidence="16">
        <location>
            <begin position="204"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="12 13">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF20768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AAK41243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="389" mass="45060" checksum="3B53DA9D5DA61428" modified="2001-10-01" version="1">MSSEFISKVDKEARRKAASILRDKFLNLVEQLKKGEPLVMEIPMRTLSNAIYDEKRKLLLLGEKKLKRNFLDMNEAKRFMQTVLMASIIYDALVSDEYPTIRDLYYRGKHSLLLKSIDGNKIVSEENTWDEQKESDSVIVDIEVFTSLLREEMLILSKEKGKVVGNLRIRSGNDTIDLSKTGHGAYAIEPTPDLIDFIDVDAEFVLVVEKDAVFQQLHRAGFWKQYKSILITSAGQPDRATRRFVRRLNEELKLPVYILTDADPYGWYIFSVFRIGSISLSYESERLATPDAKFLGVSMSDIFGNSRKKPYLSEAERKNYIIKAKEADIKRAEEIKNYEWFKTKAWEEEINTFLHRKAKLEIEAMASKGLKFLAFQYIPEKITNKDYIA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZH5</accession>
    <name>GATD_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit D</fullName>
            <shortName evidence="1">Glu-ADT subunit D</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatD</name>
        <name type="ordered locus">SSO0938</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparaginase 1 family. GatD subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90244">
        <property type="entry name" value="F90244"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992368.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZH5"/>
    <dbReference type="SMR" id="Q97ZH5"/>
    <dbReference type="FunCoup" id="Q97ZH5">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0938"/>
    <dbReference type="PaxDb" id="273057-SSO0938"/>
    <dbReference type="EnsemblBacteria" id="AAK41213">
        <property type="protein sequence ID" value="AAK41213"/>
        <property type="gene ID" value="SSO0938"/>
    </dbReference>
    <dbReference type="GeneID" id="1455180"/>
    <dbReference type="GeneID" id="44129866"/>
    <dbReference type="KEGG" id="sso:SSO0938"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.933"/>
    <dbReference type="eggNOG" id="arCOG01924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019134_2_1_2"/>
    <dbReference type="InParanoid" id="Q97ZH5"/>
    <dbReference type="PhylomeDB" id="Q97ZH5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08962">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00586">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006033">
        <property type="entry name" value="AsnA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011878">
        <property type="entry name" value="GatD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040918">
        <property type="entry name" value="GatD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037222">
        <property type="entry name" value="GatD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00519">
        <property type="entry name" value="asnASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02153">
        <property type="entry name" value="gatD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003217">
        <property type="entry name" value="PRK04183.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18195">
        <property type="entry name" value="GatD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500175">
        <property type="entry name" value="Glu_ADT_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141300">
        <property type="entry name" value="GatD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140064" description="Glutamyl-tRNA(Gln) amidotransferase subunit D">
        <location>
            <begin position="1"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase" evidence="2">
        <location>
            <begin position="92"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01068"/>
        </source>
    </evidence>
    <sequence length="444" mass="50017" checksum="90C5734E0CAED1EC" modified="2001-10-01" version="1">MQENYKGKAYDILKNLNIEEGDLIEIKKGDLRIRGILLPSYSKDDRIFVIKLDNGYNIGISIDNITEIKPIEKKSSKDRESERREVHNGAKSEIKIISTGGTIVSRVEYETGAVRPALTTEEIIQFLPEINEIAKVDAEVLFSILSENMKPEFWVKIAESVKKAFDEGNTGIVIAHGTDTMAYTASALAFSLRSLQGPVVLVGSQRSSDRPSSDSAINLLSAVITAKYAPFGEVVVNMHAESSDTYALVHRGVKVRKMHSSRRDAFQSVNDKPLAKVLWKERKLVMLNEKYISKKDETLLDAKFDNRVFLLYYYPGLDRDFLEQMLTNTKIRGIIIAGTGLGHTSSDHIELFRKATKDGIFIGMTTQCLFGRVNMNVYTTGRQLLDAGVTPLEDMLPEVALVKLMWVLAHEQDLEKIQNLMITNLVGEINPRHTLDLFPRWSYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2025-06-18" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZJ8</accession>
    <name>TOP3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA topoisomerase 3</fullName>
            <shortName evidence="4">SsTop3</shortName>
            <shortName evidence="5">SsTopA</shortName>
            <ecNumber evidence="2 3">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">DNA topoisomerase III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 8" type="primary">topA</name>
        <name evidence="8" type="ordered locus">SSO0907</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="J. Biol. Chem." volume="287" first="30282" last="30295">
            <title>Synergic and opposing activities of thermophilic RecQ-like helicase and topoisomerase 3 proteins in Holliday junction processing and replication fork stabilization.</title>
            <authorList>
                <person name="Valenti A."/>
                <person name="De Felice M."/>
                <person name="Perugino G."/>
                <person name="Bizard A."/>
                <person name="Nadal M."/>
                <person name="Rossi M."/>
                <person name="Ciaramella M."/>
            </authorList>
            <dbReference type="PubMed" id="22722926"/>
            <dbReference type="DOI" id="10.1074/jbc.m112.366377"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH LHR</scope>
        <scope>DNA-BINDING</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2018" name="Nucleic Acids Res." volume="46" first="861" last="872">
            <title>TopA, the Sulfolobus solfataricus topoisomerase III, is a decatenase.</title>
            <authorList>
                <person name="Bizard A.H."/>
                <person name="Yang X."/>
                <person name="Debat H."/>
                <person name="Fogg J.M."/>
                <person name="Zechiedrich L."/>
                <person name="Strick T.R."/>
                <person name="Garnier F."/>
                <person name="Nadal M."/>
            </authorList>
            <dbReference type="PubMed" id="29253195"/>
            <dbReference type="DOI" id="10.1093/nar/gkx1247"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
    </reference>
    <reference evidence="11 12" key="4">
        <citation type="submission" date="2019-06" db="PDB data bank">
            <title>Crystal structures of the Sulfolobus solfataricus topoisomerase III reveal that its C-terminal novel zinc finger part is a unique decatenation domain.</title>
            <authorList>
                <person name="Wang H.Q."/>
                <person name="Zhang J.H."/>
                <person name="Zheng X."/>
                <person name="Zheng Z.F."/>
                <person name="Dong Y.H."/>
                <person name="Huang L."/>
                <person name="Gong Y."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS)</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">Relaxes negatively supercoiled (SC) DNA in an ATP-independent manner at 75 degrees Celsius (PubMed:22722926, PubMed:29253195). From 50-85 degrees Celsius relaxes negatively SC DNA; at up to 100 degrees Celsius it unlinks catenated DNA to form circular double-stranded (ds)DNA molecules (PubMed:29253195). The promotion or prevention of DNA linking/unlinking is temperature-dependent in vitro and probably depends on other factors in vivo (PubMed:29253195). One study found single-stranded binding protein (SSB) does not enhance its activity (PubMed:22722926), while another study found it does (PubMed:29253195). Binds single-stranded (ss)DNA and less well to Holliday junction (HJ) or forked DNA (PubMed:22722926). Anneals complementary oligonucleotides to form HJ structures (PubMed:22722926).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="3">Single-strand (ss)DNA regions and DNA single-stranded binding protein stimulate relaxation.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text evidence="3">Optimum temperature is 75 degrees Celsius for topoisomerase activity (PubMed:29253195). Optimum temperature for decatenase is 75-90 degrees Celsius (PubMed:29253195).</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with helicase Lhr-Core.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="2 3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90241">
        <property type="entry name" value="G90241"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992334.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="6K8N">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B/C/D=1-668"/>
    </dbReference>
    <dbReference type="PDB" id="6K8O">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A=1-668"/>
    </dbReference>
    <dbReference type="PDBsum" id="6K8N"/>
    <dbReference type="PDBsum" id="6K8O"/>
    <dbReference type="AlphaFoldDB" id="Q97ZJ8"/>
    <dbReference type="SMR" id="Q97ZJ8"/>
    <dbReference type="FunCoup" id="Q97ZJ8">
        <property type="interactions" value="192"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0907"/>
    <dbReference type="PaxDb" id="273057-SSO0907"/>
    <dbReference type="EnsemblBacteria" id="AAK41190">
        <property type="protein sequence ID" value="AAK41190"/>
        <property type="gene ID" value="SSO0907"/>
    </dbReference>
    <dbReference type="GeneID" id="1455157"/>
    <dbReference type="KEGG" id="sso:SSO0907"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.910"/>
    <dbReference type="eggNOG" id="arCOG01527">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002929_5_2_2"/>
    <dbReference type="InParanoid" id="Q97ZJ8"/>
    <dbReference type="PhylomeDB" id="Q97ZJ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03362">
        <property type="entry name" value="TOPRIM_TopoIA_TopoIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.290.10:FF:000003">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.140:FF:000006">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="entry name" value="Topoisomerase I, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005739">
        <property type="entry name" value="TopoI_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034144">
        <property type="entry name" value="TOPRIM_TopoIII"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004438">
        <property type="entry name" value="PRK05776.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01057">
        <property type="entry name" value="topA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390:SF26">
        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPO_IA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0799">Topoisomerase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000462475" description="DNA topoisomerase 3">
        <location>
            <begin position="1"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="1">
        <location>
            <begin position="158"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="1">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="602"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="605"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="615"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="618"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="11 12">
        <location>
            <begin position="4"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="8"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="32"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="39"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="51"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="62"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="68"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="76"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="80"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="85"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="104"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="115"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="131"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="134"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="144"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="159"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="203"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="237"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="250"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="turn" evidence="13">
        <location>
            <begin position="259"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="264"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="285"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="300"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="332"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="turn" evidence="14">
        <location>
            <begin position="341"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="345"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="386"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="407"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="424"/>
            <end position="434"/>
        </location>
    </feature>
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        <location>
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            <end position="439"/>
        </location>
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    <feature type="strand" evidence="13">
        <location>
            <begin position="459"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="495"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="510"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="517"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="522"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="536"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="541"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="561"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="603"/>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
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        </location>
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    <feature type="helix" evidence="13">
        <location>
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            <end position="666"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01383"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="29253195"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PubMed" id="22722926"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PubMed" id="22722926"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PubMed" id="29253195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41190.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PDB" id="6K8N"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PDB" id="6K8O"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PDB" id="6K8N"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="14">
        <source>
            <dbReference type="PDB" id="6K8O"/>
        </source>
    </evidence>
    <sequence length="668" mass="76573" checksum="9EBC050056E4F085" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZL2</accession>
    <name>Q97ZL2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphoenolpyruvate synthase</fullName>
            <shortName evidence="12">PEP synthase</shortName>
            <ecNumber evidence="12">2.7.9.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Pyruvate, water dikinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ppsA-1</name>
        <name evidence="16" type="ordered locus">SSO0883</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>pyruvate + ATP + H2O = phosphoenolpyruvate + AMP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11364"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
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    <dbReference type="EnsemblBacteria" id="AAK41168">
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    <dbReference type="GeneID" id="1455135"/>
    <dbReference type="KEGG" id="sso:SSO0883"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.889"/>
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    <dbReference type="HOGENOM" id="CLU_007308_6_2_2"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PRK06464.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43030">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43030:SF1">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000854">
        <property type="entry name" value="PEP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="14">
        <location>
            <begin position="29"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme mobile" evidence="13">
        <location>
            <begin position="383"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme C-terminal" evidence="15">
        <location>
            <begin position="482"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AAK41168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="799" mass="89393" checksum="94743CA72475C9B8" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZR4</accession>
    <name>Q97ZR4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in a partial transposon ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO6050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90198">
        <property type="entry name" value="F90198"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZR4"/>
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    <dbReference type="EnsemblBacteria" id="AAK40845">
        <property type="protein sequence ID" value="AAK40845"/>
        <property type="gene ID" value="SSO6050"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO6050"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.524"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_207591_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZR4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="63" mass="7649" checksum="A187B6A85767180D" modified="2001-10-01" version="1">MIEESFKVRKARIRYVRKLSNKIFLFLYYTVLDSAWNLVNYLLFNFKSTCKKVLSFDSFVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZS8</accession>
    <name>Q97ZS8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methylenetetrahydrofolate reductase (NAD(P)H)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0501</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90195">
        <property type="entry name" value="E90195"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991392.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZS8"/>
    <dbReference type="STRING" id="273057.SSO0501"/>
    <dbReference type="PaxDb" id="273057-SSO0501"/>
    <dbReference type="EnsemblBacteria" id="AAK40820">
        <property type="protein sequence ID" value="AAK40820"/>
        <property type="gene ID" value="SSO0501"/>
    </dbReference>
    <dbReference type="GeneID" id="1455630"/>
    <dbReference type="KEGG" id="sso:SSO0501"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.494"/>
    <dbReference type="eggNOG" id="arCOG00475">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098912_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZS8"/>
    <dbReference type="PhylomeDB" id="Q97ZS8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38755">
        <property type="entry name" value="5,10-METHYLENETETRAHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38755:SF1">
        <property type="entry name" value="METHYLENE-TETRAHYDROFOLATE REDUCTASE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25991" checksum="FAEF254CEB579685" modified="2001-10-01" version="1">MQILVELHPKKKPEKLLNEIKILSSFDGFDIPDSPMGMPSPIPTFVASLIRYSLSLDRKTIIINQRLLDVNELFIRSLSITAKMLDAQIAFTKGDKPKYGKEVGYLSSDEAVNLAKEYGVKSGMMISLRKNENEIMARLDSNADFFLVLRMKDVDQIKYYGPKLIERAIPYLIVMTDKNRELVKSLDQPSFVENEIFSIINSLDSIGVKTVLISSLGDLSFFERLHARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZU4</accession>
    <name>Q97ZU4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Translocase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO0483</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90193">
        <property type="entry name" value="E90193"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922998.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZU4"/>
    <dbReference type="STRING" id="273057.SSO0483"/>
    <dbReference type="PaxDb" id="273057-SSO0483"/>
    <dbReference type="EnsemblBacteria" id="AAK40804">
        <property type="protein sequence ID" value="AAK40804"/>
        <property type="gene ID" value="SSO0483"/>
    </dbReference>
    <dbReference type="GeneID" id="1455614"/>
    <dbReference type="KEGG" id="sso:SSO0483"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.477"/>
    <dbReference type="eggNOG" id="arCOG05911">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169419_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZU4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK40804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="11993" checksum="7C6FA22341D085C5" modified="2001-10-01" version="1">MISNLSDFLVVVVVFILLMAGDKNAGNTTKSIGRFLGEIRKRQNEFKNELMRELNSVEATNNTPFTGSNFKYVKVTNQERVKELEAQIKRLQEELERLKASDGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZW0</accession>
    <name>Q97ZW0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0465</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90191">
        <property type="entry name" value="E90191"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922982.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZW0"/>
    <dbReference type="STRING" id="273057.SSO0465"/>
    <dbReference type="PaxDb" id="273057-SSO0465"/>
    <dbReference type="EnsemblBacteria" id="AAK40788">
        <property type="protein sequence ID" value="AAK40788"/>
        <property type="gene ID" value="SSO0465"/>
    </dbReference>
    <dbReference type="GeneID" id="15298041"/>
    <dbReference type="KEGG" id="sso:SSO0465"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.459"/>
    <dbReference type="eggNOG" id="arCOG05928">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369522_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZW0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="207" mass="23800" checksum="DF77DC63B27A5FF9" modified="2001-10-01" version="1">MRFEVTVDYLQGIGRKVLTSDGHVVELNPSLEKELSLIGVSSKLFAEGLIDAVTQNNGTYSFFLPAKKISDECENVLRIFEIWISTTNQTRKMLVIIINVEGNAQITLLRPELYNDFSKDLIEILAKRYICLKITMPFMYRSVIFDTFNSFKRLFDIIFEGIINLSGNIYMATISNDKKALLWKIDSTNIRYVSNNLIPSELLRLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZZ5</accession>
    <name>Q97ZZ5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator TrmB N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0426</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90186">
        <property type="entry name" value="G90186"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988755.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZZ5"/>
    <dbReference type="STRING" id="273057.SSO0426"/>
    <dbReference type="PaxDb" id="273057-SSO0426"/>
    <dbReference type="EnsemblBacteria" id="AAK40750">
        <property type="protein sequence ID" value="AAK40750"/>
        <property type="gene ID" value="SSO0426"/>
    </dbReference>
    <dbReference type="GeneID" id="1455560"/>
    <dbReference type="KEGG" id="sso:SSO0426"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.417"/>
    <dbReference type="eggNOG" id="arCOG02037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096706_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZZ5"/>
    <dbReference type="PhylomeDB" id="Q97ZZ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="19"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="243" mass="27689" checksum="B16DD0E41A2DBCBF" modified="2001-10-01" version="1">MSSELLEETISRVSKFASIFGISKSELKVYSYLLIYGRATAREVSNKLSLPYTKVYNVLSKLEGRGWVIKIDKRPAVYEAIPLRDVWNKIKITFQEKLDEFEKQFIEPISSIFSSNMAYNIVVIPRDNIMDNALRLLKDYSKMYLVAISYQEFVRSDIIDILRANSLKAETKIIVDKSVNLPDISSAQVKRAQSLFGSGIITSDSILLVIKNNDILTGLFSNHKYLIDIGTVYFNHLWSTLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q980C2</accession>
    <name>Q980C2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">tRNA pseudouridine synthase subunit A (TruB)</fullName>
            <ecNumber evidence="2">4.2.1.70</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">truB</name>
        <name evidence="2" type="ordered locus">SSO5761</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.70" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99183">
        <property type="entry name" value="B99183"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988802.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980C2"/>
    <dbReference type="STRING" id="273057.SSO5761"/>
    <dbReference type="PaxDb" id="273057-SSO5761"/>
    <dbReference type="EnsemblBacteria" id="AAK40721">
        <property type="protein sequence ID" value="AAK40721"/>
        <property type="gene ID" value="SSO5761"/>
    </dbReference>
    <dbReference type="GeneID" id="1455531"/>
    <dbReference type="KEGG" id="sso:SSO5761"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.388"/>
    <dbReference type="eggNOG" id="arCOG06042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174504_0_0_2"/>
    <dbReference type="InParanoid" id="Q980C2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004730">
        <property type="term" value="F:pseudouridylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dyskerin-like" evidence="1">
        <location>
            <begin position="16"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="89" mass="10351" checksum="AA4DA4E49DC323AA" modified="2001-10-01" version="1">MILNDFIYKIDNFCGYKNEWKIRKDSETSDKYGYYPEKRPIEIHIKNSIINLDKPPGPTSHEVAYWVKKMLNVTKAGHGGTLEPIHWAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-02-21" modified="2026-01-28" version="148" xmlns="https://uniprot.org/uniprot">
    <accession>Q980T8</accession>
    <name>DNLI_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA ligase</fullName>
            <ecNumber evidence="1">6.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polydeoxyribonucleotide synthase [ATP]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lig</name>
        <name type="ordered locus">SSO0189</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Mol. Cell" volume="11" first="275" last="282">
            <title>A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Dionne I."/>
                <person name="Nookala R.K."/>
                <person name="Jackson S.P."/>
                <person name="Doherty A.J."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="12535540"/>
            <dbReference type="DOI" id="10.1016/S1097-2765(02)00824-9"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>INTERACTION WITH PCNA3</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF 1-MET--LEU-30</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2006" name="Mol. Cell" volume="24" first="279" last="291">
            <title>A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA.</title>
            <authorList>
                <person name="Pascal J.M."/>
                <person name="Tsodikov O.V."/>
                <person name="Hura G.L."/>
                <person name="Song W."/>
                <person name="Cotner E.A."/>
                <person name="Classen S."/>
                <person name="Tomkinson A.E."/>
                <person name="Tainer J.A."/>
                <person name="Ellenberger T."/>
            </authorList>
            <dbReference type="PubMed" id="17052461"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2006.08.015"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) IN COMPLEXES WITH ATP AND PCNA</scope>
        <scope>PARTIAL PROTEIN SEQUENCE</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF 110-PHE-LEU-111</scope>
        <scope>COFACTOR</scope>
    </reference>
    <comment type="function">
        <text evidence="2">DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Interaction with PCNA enhances ligase activity. DNA polymerase I, DNA ligase and the flap endonuclease may be constitutively associated with the PCNA heterotrimer forming a scanning complex able to couple DNA synthesis and Okazaki fragment maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Ligase activity stimulated by PCNA heterotrimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3">Interacts with the PCNA heterotrimer, probably via subunit PCNA3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90159">
        <property type="entry name" value="H90159"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990427.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2HIV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.05 A"/>
        <property type="chains" value="A=1-601"/>
    </dbReference>
    <dbReference type="PDB" id="2HIX">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.87 A"/>
        <property type="chains" value="A=1-601"/>
    </dbReference>
    <dbReference type="PDB" id="7RPO">
        <property type="method" value="EM"/>
        <property type="resolution" value="4.16 A"/>
        <property type="chains" value="E=1-601"/>
    </dbReference>
    <dbReference type="PDB" id="7RPW">
        <property type="method" value="EM"/>
        <property type="resolution" value="4.38 A"/>
        <property type="chains" value="E=1-601"/>
    </dbReference>
    <dbReference type="PDB" id="7RPX">
        <property type="method" value="EM"/>
        <property type="resolution" value="4.20 A"/>
        <property type="chains" value="E=1-601"/>
    </dbReference>
    <dbReference type="PDBsum" id="2HIV"/>
    <dbReference type="PDBsum" id="2HIX"/>
    <dbReference type="PDBsum" id="7RPO"/>
    <dbReference type="PDBsum" id="7RPW"/>
    <dbReference type="PDBsum" id="7RPX"/>
    <dbReference type="AlphaFoldDB" id="Q980T8"/>
    <dbReference type="EMDB" id="EMD-24618"/>
    <dbReference type="EMDB" id="EMD-24624"/>
    <dbReference type="EMDB" id="EMD-24625"/>
    <dbReference type="SMR" id="Q980T8"/>
    <dbReference type="FunCoup" id="Q980T8">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0189"/>
    <dbReference type="PaxDb" id="273057-SSO0189"/>
    <dbReference type="EnsemblBacteria" id="AAK40535">
        <property type="protein sequence ID" value="AAK40535"/>
        <property type="gene ID" value="SSO0189"/>
    </dbReference>
    <dbReference type="GeneID" id="1455345"/>
    <dbReference type="KEGG" id="sso:SSO0189"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.188"/>
    <dbReference type="eggNOG" id="arCOG01347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005138_6_0_2"/>
    <dbReference type="InParanoid" id="Q980T8"/>
    <dbReference type="PhylomeDB" id="Q980T8"/>
    <dbReference type="BRENDA" id="6.5.1.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q980T8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07901">
        <property type="entry name" value="Adenylation_DNA_ligase_Arch_LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07969">
        <property type="entry name" value="OBF_DNA_ligase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000007">
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        <property type="entry name" value="Probable DNA ligase"/>
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        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
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        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
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        <property type="entry name" value="ATP-dep_DNA_ligase"/>
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        <property type="entry name" value="DNA_ligae_ATP-dep_bac/arc"/>
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        <property type="entry name" value="DNA_ligase_ATP-dep"/>
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        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
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        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
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        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
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        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
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        <property type="entry name" value="DNA_ligase_N_sf"/>
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        <property type="entry name" value="NA-bd_OB-fold"/>
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    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA LIGASE 1"/>
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        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
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        <property type="entry name" value="DNA_ligase_A_C"/>
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        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
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        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA_LIGASE_A2"/>
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        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000059618" description="DNA ligase">
        <location>
            <begin position="1"/>
            <end position="601"/>
        </location>
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    <feature type="active site" description="N6-AMP-lysine intermediate">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="binding site">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site">
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            <position position="265"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="No interaction with PCNA3, no stimulation by PCNA heterotrimer." evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Impairs interaction with PCNA." evidence="3">
        <original>FL</original>
        <variation>AA</variation>
        <location>
            <begin position="110"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="4"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="19"/>
            <end position="31"/>
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    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="35"/>
            <end position="37"/>
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    <feature type="helix" evidence="4">
        <location>
            <begin position="38"/>
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            <begin position="45"/>
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        <location>
            <begin position="52"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="63"/>
            <end position="74"/>
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    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="78"/>
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    <feature type="helix" evidence="4">
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        <location>
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        <location>
            <begin position="139"/>
            <end position="153"/>
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    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="156"/>
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    <feature type="helix" evidence="4">
        <location>
            <begin position="176"/>
            <end position="187"/>
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        <location>
            <begin position="188"/>
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    </feature>
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        <location>
            <begin position="191"/>
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    <feature type="helix" evidence="4">
        <location>
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        <location>
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        <location>
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        <location>
            <begin position="444"/>
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        <location>
            <begin position="458"/>
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        <location>
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        <location>
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        <location>
            <begin position="491"/>
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    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="505"/>
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    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="520"/>
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    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="528"/>
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        <location>
            <begin position="544"/>
            <end position="548"/>
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    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="554"/>
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    <feature type="strand" evidence="4">
        <location>
            <begin position="561"/>
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    <feature type="helix" evidence="4">
        <location>
            <begin position="571"/>
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    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="577"/>
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        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12535540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="17052461"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2HIV"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="2HIX"/>
        </source>
    </evidence>
    <sequence length="601" mass="67733" checksum="DA6814F4A6F0546E" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q980U1</accession>
    <name>Q980U1_SACS2</name>
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        <submittedName>
            <fullName evidence="3">Sarcosine oxidase, subunit alpha related (SoxA), putative (SoxA-like)</fullName>
            <ecNumber evidence="3">1.5.3.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">soxA-like</name>
        <name evidence="3" type="ordered locus">SSO0186</name>
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    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
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        <property type="protein sequence ID" value="AAK40532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="E90159"/>
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        <property type="component" value="Chromosome"/>
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        <property type="term" value="F:sarcosine oxidase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="entry name" value="Metab_Enz_Cyan_OpOx_G3PDH"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="483" mass="55527" checksum="AF8D33F8759F9DCC" modified="2001-10-01" version="1">MRSSKMDMPIVLSKRHRRLRSMKDCEPGLPYNEIVENGKRIDLCYSKQYKKYTKKIEIRVGTSILHNKLLRIFPNIVKIYSKHFINLPDLITENTEKRELIFIELENLIIGGGTSGLGVLSEISKEEKTLLISSNIESNTDIPYPLPQINKDEFSKLLRDTINENKDRILEGILLGKFDEGIGFLTKDKILMVRAKRVFLATGGRYIPPIFDGNDIPGIISKNLYLRYGNRITKAIALGNSDDIIKVLFKVEKRVIINNGVLFLSKYYKELIDELGVEIINTNNLKVKREKGGMLKIITNERSFDSNILVYAIVKQPKIDHSYNIGIPYNFSEYFHIYMPIHSIEGKTNDNIYIVGGMRGISDEYTSFLSGKTAVNEKYLDEFINNLKDYTYIYDYYYQKNPKENPSPYIYGNKGYVCECEDITLSEVNRQVGRGFSKVEEIKRTIGLGTGECQGKLCTYSLGSFLGDRQLITFRTPLYRMVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-01-23" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX10</accession>
    <name>PYRF_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="1">4.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">OMP decarboxylase</fullName>
            <shortName evidence="1">OMPDCase</shortName>
            <shortName evidence="1">OMPdecase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrF</name>
        <name type="ordered locus">SSO0616</name>
        <name type="ORF">C08_034</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>orotidine 5'-phosphate + H(+) = UMP + CO2</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the OMP decarboxylase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90208">
        <property type="entry name" value="H90208"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991148.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX10"/>
    <dbReference type="SMR" id="Q9UX10"/>
    <dbReference type="FunCoup" id="Q9UX10">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0616"/>
    <dbReference type="PaxDb" id="273057-SSO0616"/>
    <dbReference type="EnsemblBacteria" id="AAK40927">
        <property type="protein sequence ID" value="AAK40927"/>
        <property type="gene ID" value="SSO0616"/>
    </dbReference>
    <dbReference type="GeneID" id="1454894"/>
    <dbReference type="KEGG" id="sso:SSO0616"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.624"/>
    <dbReference type="eggNOG" id="arCOG00081">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067069_2_0_2"/>
    <dbReference type="InParanoid" id="Q9UX10"/>
    <dbReference type="PhylomeDB" id="Q9UX10"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04725">
        <property type="entry name" value="OMP_decarboxylase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01200_A">
        <property type="entry name" value="OMPdecase_type1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047595">
        <property type="entry name" value="OMPdecase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119:SF2">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000134618" description="Orotidine 5'-phosphate decarboxylase">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01200"/>
        </source>
    </evidence>
    <sequence length="222" mass="24701" checksum="48F3E83DB0F043FF" modified="2000-05-01" version="1">MLKSRVILAMDKPLSYQVLKEMENELYGIKVGLPLVLDLGVDKTRELLIGLDVEEIIVDFKLADIGYIMKSIVERLSFANSFIAHSFIGVKGSLDELKRYLDANSKNLYLVAVMSHEGWSTLFADYIKNVIREISPKGIVVGGTKLDHITQYRRDFEKMTIVSPGMGSQGGSYGDAVCAGADYEIIGRSIYNAGNPLTALRTINKIIEDKVMKCKGAIFRKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX46</accession>
    <name>COXX_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protoheme IX farnesyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Heme B farnesyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heme O synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ctaB</name>
        <name type="ordered locus">SSO0656</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>heme b + (2E,6E)-farnesyl diphosphate + H2O = Fe(II)-heme o + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:28070"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.141"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; heme O biosynthesis; heme O from protoheme: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40961" version="1"/>
        </conflict>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAB57646" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.5.1.141" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40961.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57646.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99213">
        <property type="entry name" value="B99213"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991195.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX46"/>
    <dbReference type="SMR" id="Q9UX46"/>
    <dbReference type="FunCoup" id="Q9UX46">
        <property type="interactions" value="307"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0656"/>
    <dbReference type="PaxDb" id="273057-SSO0656"/>
    <dbReference type="EnsemblBacteria" id="AAK40961">
        <property type="protein sequence ID" value="AAK40961"/>
        <property type="gene ID" value="SSO0656"/>
    </dbReference>
    <dbReference type="GeneID" id="1454928"/>
    <dbReference type="KEGG" id="sso:SSO0656"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.658"/>
    <dbReference type="eggNOG" id="arCOG00479">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029631_0_1_2"/>
    <dbReference type="InParanoid" id="Q9UX46"/>
    <dbReference type="UniPathway" id="UPA00834">
        <property type="reaction ID" value="UER00712"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048034">
        <property type="term" value="P:heme O biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00154">
        <property type="entry name" value="CyoE_CtaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448:SF2">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0350">Heme biosynthesis</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000327198" description="Protoheme IX farnesyltransferase">
        <location>
            <begin position="1"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="285" mass="31061" checksum="04D45BE29E753D73" modified="2008-04-08" version="2">MSLQQKIKAYLKLGKLGVVSLLDLAAIAGAFLAYKHGISLLPIIPMLIGGTLASMGAMIINSGIEINRDKVMSRTSNRPTVVGYVSRKEVIVVGSLLAVLGTALGFIDNALTAFFIALGVIIYVFVYTILLKPRTWLNIVIGGFAGSAAAWAGYTSLTNSLTLEAFLLGFLIFMWTPGHFWSLALKYREDYINAHYPMLPAVVGITTSARAIATSNALMIPIVLLMGYYISLIALIVFSVLSALLLFLSYRLILNPTKEEAMKSFIFSNIYLMLILLSIIIIKLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-11-02" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX49</accession>
    <name>NIKR_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative nickel-responsive regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0659</name>
        <name type="ORF">C09_008</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcriptional regulator.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="1">Binds 1 nickel ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90213">
        <property type="entry name" value="E90213"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991200.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX49"/>
    <dbReference type="SMR" id="Q9UX49"/>
    <dbReference type="STRING" id="273057.SSO0659"/>
    <dbReference type="PaxDb" id="273057-SSO0659"/>
    <dbReference type="EnsemblBacteria" id="AAK40964">
        <property type="protein sequence ID" value="AAK40964"/>
        <property type="gene ID" value="SSO0659"/>
    </dbReference>
    <dbReference type="GeneID" id="1454931"/>
    <dbReference type="KEGG" id="sso:SSO0659"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.661"/>
    <dbReference type="eggNOG" id="arCOG01008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113319_2_1_2"/>
    <dbReference type="InParanoid" id="Q9UX49"/>
    <dbReference type="PhylomeDB" id="Q9UX49"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010045">
        <property type="term" value="P:response to nickel cation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00476">
        <property type="entry name" value="NikR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050192">
        <property type="entry name" value="CopG/NikR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022988">
        <property type="entry name" value="Ni_resp_reg_NikR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719:SF2">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000139314" description="Putative nickel-responsive regulator">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00476"/>
        </source>
    </evidence>
    <sequence length="133" mass="15396" checksum="E2C9040A21234ED1" modified="2000-05-01" version="1">MSAEKISISLPKELYRELEDFIIRKGIPDRSKIFQIALRNYLDENRDGNEIIYGIINLVYDHEEASEALTEIQHEYNDSIISTLHLHVSEKICIEAIAVKGEKRKLVELNNKLGQIRGILKARLLISFPYEKT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2025-06-18" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXF3</accession>
    <name>PSB2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome subunit beta 2</fullName>
            <ecNumber evidence="1">3.4.25.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">20S proteasome beta subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Proteasome core protein PsmB 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">psmB2</name>
        <name type="ordered locus">SSO0766</name>
        <name type="ORF">C40_002</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK41063" version="1"/>
        </conflict>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAB57537" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57537.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41063.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90225">
        <property type="entry name" value="H90225"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXF3"/>
    <dbReference type="SMR" id="Q9UXF3"/>
    <dbReference type="FunCoup" id="Q9UXF3">
        <property type="interactions" value="246"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0766"/>
    <dbReference type="MEROPS" id="T01.002"/>
    <dbReference type="PaxDb" id="273057-SSO0766"/>
    <dbReference type="EnsemblBacteria" id="AAK41063">
        <property type="protein sequence ID" value="AAK41063"/>
        <property type="gene ID" value="SSO0766"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0766"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.765"/>
    <dbReference type="eggNOG" id="arCOG00970">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_7_2_2"/>
    <dbReference type="InParanoid" id="Q9UXF3"/>
    <dbReference type="PhylomeDB" id="Q9UXF3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03764">
        <property type="entry name" value="proteasome_beta_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000049">
        <property type="entry name" value="Proteasome subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02113_A">
        <property type="entry name" value="Proteasome_B_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019983">
        <property type="entry name" value="Pept_T1A_Psome_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03634">
        <property type="entry name" value="arc_protsome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-0647">Proteasome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0888">Threonine protease</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_0000026673" description="Removed in mature form; by autocatalysis" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000026674" description="Proteasome subunit beta 2">
        <location>
            <begin position="15"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="208" mass="22927" checksum="C940A8273AB25CB9" modified="2000-12-01" version="2" precursor="true">MGNELQLENKILKGTTTVGIRVNDGVILAADRRASAGFFVANKMVRKVLYITDKIGITTAGSVADLQFIYDVLKNIYHYNSITKYGPISIKGIATRLANVLSATKYFPYIVQILIGGYDDQPRLFNLDYLGDITEENYVATGSGSPVAMGVLEDEYNPKMTLDEAADLAKRAVFSAIKRDSFTGTGVIVAKIHSKGHEELEFYLNKKM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1994-02-01" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>P35027</accession>
    <name>RS10_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein uS10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">30S ribosomal protein S10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 8" type="primary">rps10</name>
        <name evidence="6" type="synonym">rps10AB</name>
        <name type="ordered locus">SSO0215</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1994" name="Biochem. Mol. Biol. Int." volume="33" first="927" last="937">
            <title>Organization of a Sulfolobus solfataricus gene cluster homologous to the Escherichia coli str operon.</title>
            <authorList>
                <person name="Ianniciello G."/>
                <person name="Gallo M."/>
                <person name="Arcari P."/>
                <person name="Bocchini V."/>
            </authorList>
            <dbReference type="PubMed" id="7527264"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="journal article" date="1993" name="Nucleic Acids Res." volume="21" first="1666" last="1666">
            <title>Primary structure of the elongation factor 1 alpha in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Arcari P."/>
                <person name="Gallo M."/>
                <person name="Ianniciello G."/>
                <person name="Dello Russo A."/>
                <person name="Bocchini V."/>
            </authorList>
            <dbReference type="PubMed" id="8369039"/>
            <dbReference type="DOI" id="10.1093/nar/21.7.1666"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference evidence="9 10 11" key="4">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="function">
        <text evidence="1">Involved in the binding of tRNA to the ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="X76767">
        <property type="protein sequence ID" value="CAA54163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X70701">
        <property type="protein sequence ID" value="CAA50034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="T11748">
        <property type="entry name" value="T11748"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990464.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="L=1-102"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="L=1-102"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="L=1-102"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="L=1-102"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="L=1-102"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="L=1-102"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="L=1-102"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="L=1-102"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="P35027"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="P35027"/>
    <dbReference type="FunCoup" id="P35027">
        <property type="interactions" value="220"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0215"/>
    <dbReference type="PaxDb" id="273057-SSO0215"/>
    <dbReference type="EnsemblBacteria" id="AAK40558">
        <property type="protein sequence ID" value="AAK40558"/>
        <property type="gene ID" value="SSO0215"/>
    </dbReference>
    <dbReference type="GeneID" id="42362087"/>
    <dbReference type="KEGG" id="sso:SSO0215"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.213"/>
    <dbReference type="eggNOG" id="arCOG01758">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122625_0_1_2"/>
    <dbReference type="InParanoid" id="P35027"/>
    <dbReference type="PhylomeDB" id="P35027"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000004">
        <property type="entry name" value="30S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_uS10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005729">
        <property type="entry name" value="Ribosomal_uS10_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01046">
        <property type="entry name" value="uS10_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000146660" description="Small ribosomal subunit protein uS10">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="7527264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="7">
        <source>
            <dbReference type="EMBL" id="CAA50034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="8">
        <source>
            <dbReference type="EMBL" id="CAA54163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="10">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="11">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="102" mass="12112" checksum="27D270026A28C3E4" modified="1995-02-01" version="2">MPTKARIRLWSTNVENLNYVITQIRGIVEKTGIEMRGPIPLPTSKLEVPIMRLPHGEGRKKWEKWEMRVHKRLIDIAADERVMRQLMRVRVPEDVYIEIQLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2025-06-18" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>P50317</accession>
    <accession>Q9UWT3</accession>
    <name>PGK_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Phosphoglycerate kinase</fullName>
            <ecNumber>2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">pgk</name>
        <name type="ordered locus">SSO0527</name>
        <name type="ORF">C22_024</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1995" name="Eur. J. Biochem." volume="233" first="800" last="808">
            <title>The phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from the thermophilic archaeon Sulfolobus solfataricus overlap by 8-bp. Isolation, sequencing of the genes and expression in Escherichia coli.</title>
            <authorList>
                <person name="Jones C.E."/>
                <person name="Fleming T.M."/>
                <person name="Cowan D.A."/>
                <person name="Littlechild J.A."/>
                <person name="Piper P.W."/>
            </authorList>
            <dbReference type="PubMed" id="8521845"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1995.800_3.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40847" version="1"/>
        </conflict>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAB57772" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.7.2.3"/>
    <dbReference type="EMBL" id="X80178">
        <property type="protein sequence ID" value="CAA56459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57772.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40847.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90198">
        <property type="entry name" value="H90198"/>
    </dbReference>
    <dbReference type="PIR" id="S63528">
        <property type="entry name" value="S63528"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P50317"/>
    <dbReference type="SMR" id="P50317"/>
    <dbReference type="FunCoup" id="P50317">
        <property type="interactions" value="294"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0527"/>
    <dbReference type="PaxDb" id="273057-SSO0527"/>
    <dbReference type="EnsemblBacteria" id="AAK40847">
        <property type="protein sequence ID" value="AAK40847"/>
        <property type="gene ID" value="SSO0527"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0527"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.526"/>
    <dbReference type="eggNOG" id="arCOG00496">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025427_0_2_2"/>
    <dbReference type="InParanoid" id="P50317"/>
    <dbReference type="PhylomeDB" id="P50317"/>
    <dbReference type="BRENDA" id="2.7.2.3">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="P50317"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000006">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000012">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF23">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000146071" description="Phosphoglycerate kinase">
        <location>
            <begin position="1"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="24"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="63"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="357"/>
            <end position="360"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="408" mass="44396" checksum="9EBF90068315C099" modified="1996-10-01" version="1">MGDLTIPTMDDIDIQSKKVLLRIDINSPVDENGKIIDDSRIKAHIGTIKELINKGNSVVLISHQGRPGDKDFTSLEEHAKLISKYLDREVIFVDDVIGPYAREMIKKLENNGILLLDNIRLISEELIEAPPQQHVKSFLVKKLAPLFDIYINDAFATAHRSQPSIVGFPLALPSAAGRVMEREVSALAKIFNAEDTPKIFTLGGGKVHDTIRIIENLVRKRIADRILTGGLVAELFSVAKGMNLNPKNMEILEKLGILSLIPRARKLLLSGAPIEIPVDYKVEINGNVIEEPASKVTGIIKDIGSTTAEIYSSFIKDAKVVVLRGPMGVIEDERFKSGSKSVLKASLEGQGYVIIGGGHMISALDEDMKIDSSKVHISTGGGALLLFLSGERLPALEALSMSVVNSGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-04-27" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>P58032</accession>
    <name>FLPA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase</fullName>
            <shortName>FIB</shortName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">flpA</name>
        <name type="ordered locus">SSO0940</name>
        <name type="ORF">C33_014</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="5289" last="5294">
            <title>In vitro reconstitution and activity of a C/D box methylation guide ribonucleoprotein complex.</title>
            <authorList>
                <person name="Omer A.D."/>
                <person name="Ziesche S."/>
                <person name="Ebhardt H."/>
                <person name="Dennis P.P."/>
            </authorList>
            <dbReference type="PubMed" id="11959980"/>
            <dbReference type="DOI" id="10.1073/pnas.082101999"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>MUTAGENESIS OF ALA-85 AND PRO-129</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2004" name="Mol. Microbiol." volume="54" first="980" last="993">
            <title>RNA-guided nucleotide modification of ribosomal and non-ribosomal RNAs in Archaea.</title>
            <authorList>
                <person name="Ziesche S.M."/>
                <person name="Omer A.D."/>
                <person name="Dennis P.P."/>
            </authorList>
            <dbReference type="PubMed" id="15522081"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.2004.04319.x"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2009" name="Proc. Natl. Acad. Sci. U.S.A." volume="106" first="13808" last="13813">
            <title>Structural organization of box C/D RNA-guided RNA methyltransferase.</title>
            <authorList>
                <person name="Ye K."/>
                <person name="Jia R."/>
                <person name="Lin J."/>
                <person name="Ju M."/>
                <person name="Peng J."/>
                <person name="Xu A."/>
                <person name="Zhang L."/>
            </authorList>
            <dbReference type="PubMed" id="19666563"/>
            <dbReference type="DOI" id="10.1073/pnas.0905128106"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) IN COMPLEXES WITH RNA; S-ADENOSYL-L-METHIONINE; NOP5 AND RPL7AE</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2011" name="Nature" volume="469" first="559" last="563">
            <title>Structural basis for site-specific ribose methylation by box C/D RNA protein complexes.</title>
            <authorList>
                <person name="Lin J."/>
                <person name="Lai S."/>
                <person name="Jia R."/>
                <person name="Xu A."/>
                <person name="Zhang L."/>
                <person name="Lu J."/>
                <person name="Ye K."/>
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        <scope>X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS) IN COMPLEX WITH GUIDE RNA; SUBSTRATE RNA AND RPL7AE</scope>
        <scope>SUBUNIT</scope>
        <scope>FUNCTION</scope>
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        <text evidence="1 2 3 5">Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 4 5">Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA. These sRNP particles form homodimers, giving rise to an asymmetric holoenzyme.</text>
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    <feature type="mutagenesis site" description="Loss of methyltransferase activity." evidence="2">
        <original>A</original>
        <variation>V</variation>
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    <feature type="mutagenesis site" description="Decreased methyltransferase activity." evidence="2">
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    <sequence length="232" mass="26422" checksum="CFFB34D2D4FA9CF2" modified="2001-04-27" version="1">MSEVITVKQTNMENIYECEFNDGSFRLCTRNLVPNFNVYGERLIKYEGVEYREWNAFRSKLAGAILKGLKTNPIRKGTKVLYLGAASGTTISHVSDIIELNGKAYGVEFSPRVVRELLLVAQRRPNIFPLLADARFPQSYKSVVENVDVLYVDIAQPDQTDIAIYNAKFFLKVNGDMLLVIKARSIDVTKDPKEIYKTEVEKLENSNFETIQIINLDPYDKDHAIVLSKYKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>P95896</accession>
    <name>AMID_SACS2</name>
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        <recommendedName>
            <fullName>Amidase</fullName>
            <ecNumber>3.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2122</name>
        <name type="ORF">C02016</name>
        <name type="ORF">C02_017</name>
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    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Extremophiles" volume="5" first="183" last="192">
            <title>Molecular and biochemical characterization of the recombinant amidase from hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="d'Abusco A.S."/>
                <person name="Ammendola S."/>
                <person name="Scandurra R."/>
                <person name="Politi L."/>
            </authorList>
            <dbReference type="PubMed" id="11453462"/>
            <dbReference type="DOI" id="10.1007/s007920100190"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>CHARACTERIZATION</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Enantioselective, active on 2- to 6-carbon aliphatic amides and on many aromatic amides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a monocarboxylic acid amide + H2O = a monocarboxylate + NH4(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35757"/>
            <dbReference type="ChEBI" id="CHEBI:83628"/>
            <dbReference type="EC" id="3.5.1.4"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text>Active from pH 4 to 9.</text>
        </phDependence>
        <temperatureDependence>
            <text>Active from 60 to 95 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text>Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.4"/>
    <dbReference type="EMBL" id="AF290611">
        <property type="protein sequence ID" value="AAK83092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y08256">
        <property type="protein sequence ID" value="CAA69448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74034">
        <property type="entry name" value="S74034"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990155.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95896"/>
    <dbReference type="SMR" id="P95896"/>
    <dbReference type="STRING" id="273057.SSO2122"/>
    <dbReference type="PaxDb" id="273057-SSO2122"/>
    <dbReference type="EnsemblBacteria" id="AAK42301">
        <property type="protein sequence ID" value="AAK42301"/>
        <property type="gene ID" value="SSO2122"/>
    </dbReference>
    <dbReference type="GeneID" id="1453628"/>
    <dbReference type="KEGG" id="sso:SSO2122"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2212"/>
    <dbReference type="eggNOG" id="arCOG01717">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_18_1_2"/>
    <dbReference type="InParanoid" id="P95896"/>
    <dbReference type="PhylomeDB" id="P95896"/>
    <dbReference type="BRENDA" id="3.5.1.4">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="3.5.2.B2">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="3.5.5.1">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.60">
        <property type="entry name" value="Glu-tRNAGln amidotransferase C subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005565">
        <property type="entry name" value="PRK07235.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF170">
        <property type="entry name" value="AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000105263" description="Amidase">
        <location>
            <begin position="1"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="1">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="504" mass="55655" checksum="A9103AB7D09A88EE" modified="1997-05-01" version="1">MGIKLPTLEDLREISKQFNLDLEDEELKSFLQLLKLQLESYERLDSLPDYTPRVKYQRTLGRPPMKEENPYGALVWITSIKGKEEGKLKGKRICIKDNVMIAGIPMLNGSKMLEGFVPHMDATVVSRILDEAGEIVAKTTCEDLCFSGGSHTSYPWPVLNPRNPEYMAGGSSSGSAVAVASGYCDMAVGGDQGGSIRIPSSWVGIYGLKPTHGLVPYTGAFSIEPTLDHLGPMANTVKDVALLLEVIAGRDELDSRQPDSLPPPPVKPYSKLIDGDVKDMKVGIVKEGFNWSNSEKDVDELVLDSAKKLEDYGIKVEDTSIPLHRMGLDIWTPIAIEGATATMILGSGVGWGRKGLFETQIADFFGNSLKSRARDLPNTVKGVLMLGYLMIKMYNNRYYAKARNLSIVLKEAYDSALRKYDALIMPTTPMKAMRYKSEPGFDEYFIMALGMINNTAPFDVTGHPAMNIPVGYSNGLPVGLMIIGRHFEEDKVLKLANVFERIKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>P95992</accession>
    <name>RS9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein uS9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>30S ribosomal protein S9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Ribosomal protein S9/S16</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">SSU ribosomal protein S9AB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 6" type="primary">rps9</name>
        <name evidence="5" type="synonym">rps9AB</name>
        <name type="ordered locus">SSO0068</name>
        <name type="ORF">C05003</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7 8 9" key="3">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). uS9, uS13 and uS19 stabilize the codon:anticodon duplex (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S small ribosomal subunit, interacts with aS33.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40430" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAA69534" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69534.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40430.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75420">
        <property type="entry name" value="S75420"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988877.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="K=1-137"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="K=1-137"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="K=1-137"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="K=1-137"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="K=1-137"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="K=1-137"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="K=1-137"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="K=1-137"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="P95992"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="P95992"/>
    <dbReference type="FunCoup" id="P95992">
        <property type="interactions" value="144"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0068"/>
    <dbReference type="PaxDb" id="273057-SSO0068"/>
    <dbReference type="EnsemblBacteria" id="AAK40430">
        <property type="protein sequence ID" value="AAK40430"/>
        <property type="gene ID" value="SSO0068"/>
    </dbReference>
    <dbReference type="GeneID" id="1455321"/>
    <dbReference type="KEGG" id="sso:SSO0068"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.69"/>
    <dbReference type="eggNOG" id="arCOG04243">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046483_4_0_2"/>
    <dbReference type="InParanoid" id="P95992"/>
    <dbReference type="PhylomeDB" id="P95992"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000051">
        <property type="entry name" value="30S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_A">
        <property type="entry name" value="Ribosomal_uS9_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019958">
        <property type="entry name" value="Ribosomal_uS9_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001749">
        <property type="entry name" value="PRK00474.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03627">
        <property type="entry name" value="uS9_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000111475" description="Small ribosomal subunit protein uS9">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="CAA69534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="137" mass="15705" checksum="127B4E7C72F8B410" modified="2002-01-23" version="3">MSEEQKLVISSARRKTARATCYIYAGKGRVFVNNVPIELIPIEMVRLKIMEPLLLAGNDIRSKIDAKIITYGGGIMGQADAARMALARALVKFTGSKELEKIYRAYDRTMLAGDPRQTESEKWMRYSARRWRQKSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX50</accession>
    <name>Q7LX50_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74062">
        <property type="entry name" value="S74062"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992162.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX50"/>
    <dbReference type="STRING" id="273057.SSO2155"/>
    <dbReference type="PaxDb" id="273057-SSO2155"/>
    <dbReference type="EnsemblBacteria" id="AAK42331">
        <property type="protein sequence ID" value="AAK42331"/>
        <property type="gene ID" value="SSO2155"/>
    </dbReference>
    <dbReference type="GeneID" id="1453658"/>
    <dbReference type="KEGG" id="sso:SSO2155"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2248"/>
    <dbReference type="eggNOG" id="arCOG06021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2127943_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX50"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="116" mass="13850" checksum="E5C262F50174A38A" modified="2011-05-31" version="1">MDKYKLALLGEAGAAGLDRGFSIRYKIFRESYSNEMYHWKYFQKYRRSFLEKPVYYVFSILGFIISLFGIEAVKKVNEIVERNAIDFYKNNFNEDDEDIMRILEDEKKHFAMSVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX88</accession>
    <name>Q7LX88_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74020">
        <property type="entry name" value="S74020"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX88"/>
    <dbReference type="PaxDb" id="273057-SSO2108"/>
    <dbReference type="DNASU" id="1453614"/>
    <dbReference type="EnsemblBacteria" id="AAK42287">
        <property type="protein sequence ID" value="AAK42287"/>
        <property type="gene ID" value="SSO2108"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2108"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2194"/>
    <dbReference type="HOGENOM" id="CLU_2257491_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX88"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="107" mass="12405" checksum="FAADDA3070070636" modified="2011-05-31" version="1">MAHGVNIYNVYSISIIALLASSFLLYAQEAKGLENAMLFLSIIFPYAFYLNGHILLYLVLPAYLIVTSSVTFHYYIYELIPTLIYYFGILPKFINYNVRLMTILWES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX91</accession>
    <name>Q7LX91_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fourth ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74016">
        <property type="entry name" value="S74016"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX91"/>
    <dbReference type="STRING" id="273057.SSO2104"/>
    <dbReference type="PaxDb" id="273057-SSO2104"/>
    <dbReference type="EnsemblBacteria" id="AAK42283">
        <property type="protein sequence ID" value="AAK42283"/>
        <property type="gene ID" value="SSO2104"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2104"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2187"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074024_2_0_2"/>
    <dbReference type="InParanoid" id="Q7LX91"/>
    <dbReference type="PhylomeDB" id="Q7LX91"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="109" mass="12828" checksum="15C47E29092DB89A" modified="2011-05-31" version="1">MFTSPGVQISIYPRIRSWSNKVRNPIETSYKSVKSFLPFTCSTKFIFRMLIFLLDVLVYSLYTILKDNVKMRTFKSLISKNIENISEIEIYLSKLEETLTNTTGLFLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXN7</accession>
    <name>Q7LXN7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90214">
        <property type="entry name" value="H90214"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991218.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXN7"/>
    <dbReference type="SMR" id="Q7LXN7"/>
    <dbReference type="STRING" id="273057.SSO0673"/>
    <dbReference type="PaxDb" id="273057-SSO0673"/>
    <dbReference type="EnsemblBacteria" id="AAK40975">
        <property type="protein sequence ID" value="AAK40975"/>
        <property type="gene ID" value="SSO0673"/>
    </dbReference>
    <dbReference type="GeneID" id="1454942"/>
    <dbReference type="KEGG" id="sso:SSO0673"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.673"/>
    <dbReference type="eggNOG" id="arCOG01776">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1207639_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXN7"/>
    <dbReference type="PhylomeDB" id="Q7LXN7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="86"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="232" mass="26194" checksum="A90B3CB6ED28EEAB" modified="2011-05-31" version="1">MIFSCPIDRSDVNEKLECEKGHKFGIIGGQIYDFLLKDVEVDKLLSRVIPIYESIWAPLGLLITSGKTYTSFLREIGEFIDGDLIIDVGTGTGKIFDFLNCKTCIGIDVSLRFLMYMKRKRTRVIAVRADANNLPLKSGIADGISSTLVLHMLSNPSFAIREMSRVLKSNGKCSIAVLANVNSIIGKTLSRWWKVNLRHYDYYINLLQENSLKVVERKELGPWEVINCIKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXR7</accession>
    <name>Q7LXR7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0621</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90209">
        <property type="entry name" value="E90209"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991155.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXR7"/>
    <dbReference type="STRING" id="273057.SSO0621"/>
    <dbReference type="PaxDb" id="273057-SSO0621"/>
    <dbReference type="EnsemblBacteria" id="AAK40932">
        <property type="protein sequence ID" value="AAK40932"/>
        <property type="gene ID" value="SSO0621"/>
    </dbReference>
    <dbReference type="GeneID" id="1454899"/>
    <dbReference type="KEGG" id="sso:SSO0621"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.629"/>
    <dbReference type="eggNOG" id="arCOG07225">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160443_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXR7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="130" mass="15058" checksum="FDB6DA778BE57D63" modified="2011-05-31" version="1">MTLSDVILRYLLSEEPIIEINENDISAEEFSSIDEISIGLRIIIIGKNRRRRLVDLGLLQIIAKCGHLDFIRDYLNMKFTLRDIYTKYNVYTELEYLAIQEDCAGLVNDLDLKCVLLKVKSTTEKRGTTR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2014-05-14" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXU0</accession>
    <accession>Q9UWX8</accession>
    <name>HJC_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Crossover junction endodeoxyribonuclease Hjc</fullName>
            <shortName evidence="2">Hjc</shortName>
            <ecNumber evidence="2">3.1.21.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Holliday junction resolvase Hjc</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hjc</name>
        <name type="ordered locus">SSO0575</name>
        <name type="ORF">ORF-c21_024</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>FUNCTION</scope>
        <scope>DNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2000" name="J. Biol. Chem." volume="275" first="25540" last="25546">
            <title>A conserved nuclease domain in the archaeal Holliday junction resolving enzyme Hjc.</title>
            <authorList>
                <person name="Kvaratskhelia M."/>
                <person name="Wardleworth B.N."/>
                <person name="Norman D.G."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="10940317"/>
            <dbReference type="DOI" id="10.1074/jbc.M003420200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>DNA-BINDING</scope>
        <scope>MUTAGENESIS OF GLU-12; ASP-42; GLU-55 AND LYS-57</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2000" name="J. Mol. Biol." volume="297" first="923" last="932">
            <title>Two Holliday junction resolving enzymes in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Kvaratskhelia M."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="10736227"/>
            <dbReference type="DOI" id="10.1006/jmbi.2000.3624"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>DNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="2992" last="2996">
            <title>Holliday junction resolution is modulated by archaeal chromatin components in vitro.</title>
            <authorList>
                <person name="Kvaratskhelia M."/>
                <person name="Wardleworth B.N."/>
                <person name="Bond C.S."/>
                <person name="Fogg J.M."/>
                <person name="Lilley D.M."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="11709558"/>
            <dbReference type="DOI" id="10.1074/jbc.M109496200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>DNA-BINDING</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="5442" last="5451">
            <title>Substrate recognition and catalysis by the Holliday junction resolving enzyme Hje.</title>
            <authorList>
                <person name="Middleton C.L."/>
                <person name="Parker J.L."/>
                <person name="Richard D.J."/>
                <person name="White M.F."/>
                <person name="Bond C.S."/>
            </authorList>
            <dbReference type="PubMed" id="15479781"/>
            <dbReference type="DOI" id="10.1093/nar/gkh869"/>
        </citation>
        <scope>MUTAGENESIS OF SER-32</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2006" name="J. Mol. Biol." volume="364" first="243" last="247">
            <title>PCNA activates the Holliday junction endonuclease Hjc.</title>
            <authorList>
                <person name="Dorazi R."/>
                <person name="Parker J.L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="17011573"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2006.09.011"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>INTERACTION WITH PCNA1</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF 137-ARG--LEU-143</scope>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="5509" last="5514">
            <title>Structure of Hjc, a Holliday junction resolvase, from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Bond C.S."/>
                <person name="Kvaratskhelia M."/>
                <person name="Richard D."/>
                <person name="White M.F."/>
                <person name="Hunter W.N."/>
            </authorList>
            <dbReference type="PubMed" id="11331763"/>
            <dbReference type="DOI" id="10.1073/pnas.091613398"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="3 4 5 7 9">A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination; may have some degree of sequence preference in a mobile junction. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are subsequently ligated to produce recombinant products. Can cleave all 4 strands 3 bases 3' of the junction center. Cleaves both mobile and immobile junctions. Modifies the structure of the 4-way DNA junction, a model Holliday junction structure. The protein forms multiple complexes with 4-way DNA, suggesting more than 1 homodimer can bind to each junction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 9">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2 7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="7 9">Autoinhibits at very high concentrations, possibly because of extreme junction distortion. Inhibition (and activity at low concentrations of enzyme) is stimulated by dsDNA and Sso7d (PubMed:11709558). Activity stimulated by PCNA subunit PCNA1 (PubMed:17011573).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 6 9 10">Homodimer (Probable). Interacts with PCNA subunit PCNA1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">A second Holliday junction resolving enzyme, Hje, with different substrate specificity exists in this organism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Holliday junction resolvase Hjc family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="2"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90204">
        <property type="entry name" value="B90204"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991087.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1HH1">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.15 A"/>
        <property type="chains" value="A=1-143"/>
    </dbReference>
    <dbReference type="PDB" id="4TKD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.01 A"/>
        <property type="chains" value="A/B/C/D=1-143"/>
    </dbReference>
    <dbReference type="PDB" id="4TKK">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=1-143"/>
    </dbReference>
    <dbReference type="PDBsum" id="1HH1"/>
    <dbReference type="PDBsum" id="4TKD"/>
    <dbReference type="PDBsum" id="4TKK"/>
    <dbReference type="AlphaFoldDB" id="Q7LXU0"/>
    <dbReference type="SMR" id="Q7LXU0"/>
    <dbReference type="STRING" id="273057.SSO0575"/>
    <dbReference type="PaxDb" id="273057-SSO0575"/>
    <dbReference type="EnsemblBacteria" id="AAK40889">
        <property type="protein sequence ID" value="AAK40889"/>
        <property type="gene ID" value="SSO0575"/>
    </dbReference>
    <dbReference type="GeneID" id="1454856"/>
    <dbReference type="GeneID" id="44129578"/>
    <dbReference type="KEGG" id="sso:SSO0575"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.583"/>
    <dbReference type="eggNOG" id="arCOG00919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139546_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LXU0"/>
    <dbReference type="PhylomeDB" id="Q7LXU0"/>
    <dbReference type="BRENDA" id="3.1.21.10">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q7LXU0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00523">
        <property type="entry name" value="Holliday_junction_resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01490">
        <property type="entry name" value="HJ_Resolv_Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002732">
        <property type="entry name" value="Hjc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014428">
        <property type="entry name" value="Hjc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040854">
        <property type="entry name" value="Hol_resolv_Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651">
        <property type="entry name" value="HOLLIDAY JUNCTION RESOLVASE HJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651:SF1">
        <property type="entry name" value="HOLLIDAY JUNCTION RESOLVASE HJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01870">
        <property type="entry name" value="Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004985">
        <property type="entry name" value="Hlld_jn_rslvs_ar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000429158" description="Crossover junction endodeoxyribonuclease Hjc">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No cleavage of 4-way junction DNA. Binds junction DNA normally." evidence="5">
        <original>E</original>
        <variation>Q</variation>
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No cleavage of fixed 4-way junction DNA. Binds junction DNA normally." evidence="8">
        <original>S</original>
        <variation>A</variation>
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No cleavage of 4-way junction DNA. Binds junction DNA normally." evidence="5">
        <original>D</original>
        <variation>N</variation>
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No cleavage of 4-way junction DNA. Binds junction DNA normally." evidence="5">
        <original>E</original>
        <variation>Q</variation>
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="1000-fold less cleavage of 4-way junction DNA. Binds junction DNA normally." evidence="5">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No interaction with PCNA1." evidence="9">
        <location>
            <begin position="137"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="25"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="42"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="50"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="65"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="86"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="94"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="98"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="105"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="113"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="10701121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="10736227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="10940317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="11331763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="11709558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="8">
        <source>
            <dbReference type="PubMed" id="15479781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="9">
        <source>
            <dbReference type="PubMed" id="17011573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="10"/>
    <evidence type="ECO:0007829" key="11">
        <source>
            <dbReference type="PDB" id="4TKD"/>
        </source>
    </evidence>
    <sequence length="143" mass="16010" checksum="22099074AECF1279" modified="2011-05-31" version="1">MNAKKRKGSAVERNIVSRLRDKGFAVVRAPASGSKRKDPIPDIIALKNGVIILIEMKSRKDIEGKIYVRREQAEGIIEFARKSGGSLFLGVKKPGVLKFIPFEKLRRTETGNYVADSEIEGLDLEDLVRLVEAKISRTLDNFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXV6</accession>
    <name>Q7LXV6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90200">
        <property type="entry name" value="F90200"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXV6"/>
    <dbReference type="STRING" id="273057.SSO0543"/>
    <dbReference type="PaxDb" id="273057-SSO0543"/>
    <dbReference type="EnsemblBacteria" id="AAK40861">
        <property type="protein sequence ID" value="AAK40861"/>
        <property type="gene ID" value="SSO0543"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0543"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.547"/>
    <dbReference type="eggNOG" id="arCOG08557">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_770788_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXV6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="41133" checksum="4E1118E968173D8C" modified="2011-05-31" version="1">MNVMKKYVLTYRSIISFFVGFMSFAFIALMKISGLSGFQIGLILSTISISQFIAYIILIAFSIRRNILVSAALELLIPIILIIQRSAFTFTLSAIFMGIVNSINSSVIIIQKEFTRKDYSIMMTLTYALSLAGLGIFYLSKIIGINTTLLLLFIPLLSLNLILTLMLRYEEKKESLSELYSWLKNVGKSVLVFGFFASLRRVLITSYIPLILLTLFYTYTPQEISLYLMIIQVPLIIFFYLGHKISVRIFITLSIVELILFLSLSIFYRESILLFLSLILLANMSSSLRAPVVEETVNKLLNFSTKISSFYHLMDTIFSALASILLAFMVKLDLFYGIFVIAGLSSLLPSLFVYKVMLQQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXW7</accession>
    <name>Q7LXW7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1234</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0524</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90198">
        <property type="entry name" value="D90198"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXW7"/>
    <dbReference type="STRING" id="273057.SSO0524"/>
    <dbReference type="PaxDb" id="273057-SSO0524"/>
    <dbReference type="EnsemblBacteria" id="AAK40843">
        <property type="protein sequence ID" value="AAK40843"/>
        <property type="gene ID" value="SSO0524"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0524"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.520"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075479_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXW7"/>
    <dbReference type="PhylomeDB" id="Q7LXW7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="312" mass="36136" checksum="AD8612C65B8EAC5E" modified="2011-05-31" version="1">MTRTLRNVPNLMYYPLPPIEDMPWREKWLTEIKPVLDTMDLERVLGEGALVYLKLLVVMVLYSCSYRDAVKMVNVNVVVAWFVGRKVGKSTLHDFVGRLYGVRKKLLEISFKLEEKCLPSYLPASAHLVDFMAWLVDSFLLDLPPGKRSVETFREKAELERREGNLERARKLLSLGRTKRRFEGRWTKKRGVSHYGLKAVAVISVSLFVRSITVKPANFSDKRFKSPLKGIKIADRGFSPSPTQLIAREKPFTTLRVHVEFFGTYLNAFWRPYGTTTWRNDVFLHVLGVIYNIKMFLAIQRRTPPGRRAVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXZ1</accession>
    <name>Q7LXZ1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HD/PDEase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75393">
        <property type="entry name" value="S75393"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988908.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXZ1"/>
    <dbReference type="FunCoup" id="Q7LXZ1">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0095"/>
    <dbReference type="PaxDb" id="273057-SSO0095"/>
    <dbReference type="EnsemblBacteria" id="AAK40452">
        <property type="protein sequence ID" value="AAK40452"/>
        <property type="gene ID" value="SSO0095"/>
    </dbReference>
    <dbReference type="GeneID" id="1453280"/>
    <dbReference type="KEGG" id="sso:SSO0095"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.92"/>
    <dbReference type="eggNOG" id="arCOG04430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026821_3_0_2"/>
    <dbReference type="InParanoid" id="Q7LXZ1"/>
    <dbReference type="PhylomeDB" id="Q7LXZ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000072">
        <property type="entry name" value="Metal dependent phosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
        <property type="entry name" value="HD_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="1">
        <location>
            <begin position="46"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="399" mass="47180" checksum="63AD603AA529E5DE" modified="2011-05-31" version="1">MKKVYDEIHAYIELDDREAKIIDMPEFQRLRRIKQTSLAYLVYPGATHTRFSHSLGTFYLTTILGEKFRQLGIITDEESTYLKLSALLHDIGQFPFSHSLEPLYLEKGLSNKDLRYMIISKSPNFREFFDNESIDYSKIIEILNGNSMMSSIVNSDVDVDRMDYLVRDSRHTGVQLGNIDLYRLLDTIFYGNNNEIVVQDKGIYSLENFFISRLHMYQAVYYHKTIIGYELMLREIFRTIYDCCDSSILSVENIRGLVYDSSISYWDDEWVFMILYTYLYSSNSPLYLKQKIRNFLDRRGPKVVYEEISYDNEMKGGDIKIKEIVDRLERNQIPRSSIYPIEEKIKILNKDKINIISKNNEMNIIRYKSTLINHIPETLTIRRIYVDHEYAKKARDVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-06-18" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LY02</accession>
    <name>Q7LY02_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Isopentenyl phosphate kinase</fullName>
            <ecNumber evidence="2">2.7.4.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">SSO0064</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>isopentenyl phosphate + ATP = isopentenyl diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:33963"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the isopentenyl phosphate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.26" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75424">
        <property type="entry name" value="S75424"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LY02"/>
    <dbReference type="FunCoup" id="Q7LY02">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0064"/>
    <dbReference type="PaxDb" id="273057-SSO0064"/>
    <dbReference type="EnsemblBacteria" id="AAK40425">
        <property type="protein sequence ID" value="AAK40425"/>
        <property type="gene ID" value="SSO0064"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0064"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.64"/>
    <dbReference type="eggNOG" id="arCOG00860">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070213_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LY02"/>
    <dbReference type="PhylomeDB" id="Q7LY02"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102043">
        <property type="term" value="F:isopentenyl phosphate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04241">
        <property type="entry name" value="AAK_FomA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024192">
        <property type="entry name" value="Fosfomycin_R_FomA-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040647">
        <property type="entry name" value="IPPK_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF1">
        <property type="entry name" value="ISOPENTENYL PHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016496">
        <property type="entry name" value="Kin_FomA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="12">
        <location>
            <begin position="14"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="17"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="11">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAK40425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="241" mass="26584" checksum="6BA97F0CC8FA46C9" modified="2011-05-31" version="1">MEMDMGSELGYDYRVLKLGGSLITCKDVPRCVKLEVLRRVSEEIRKFVNENPDKKIILLHGGGSFGHYEASIFDDNRIVRTSEAMQELNYIVAKHLLKSGIKAISVPGKFYTFDAVLSALEKDLVPLIYGDVKFDGSIISADDMSIDIAKRLNARLLFAIDKAGIIGRGGGVISELRGIDEVSILMQTNYYDITGGILSKIKKIFENNLNALIFDGSKTGNIYLALRGYNIGTLVRGNPNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TV2</accession>
    <name>Q97TV2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">First ORF in transposon ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1704</name>
        <name evidence="3" type="ordered locus">SSO1795</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90341">
        <property type="entry name" value="E90341"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TV2"/>
    <dbReference type="STRING" id="273057.SSO1704"/>
    <dbReference type="PaxDb" id="273057-SSO1704"/>
    <dbReference type="EnsemblBacteria" id="AAK41910">
        <property type="protein sequence ID" value="AAK41910"/>
        <property type="gene ID" value="SSO1704"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41988">
        <property type="protein sequence ID" value="AAK41988"/>
        <property type="gene ID" value="SSO1795"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1704"/>
    <dbReference type="KEGG" id="sso:SSO1795"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1754"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099723_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="1">
        <location>
            <begin position="40"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="243" mass="28270" checksum="C4FC96445A792596" modified="2001-10-01" version="1">MVEWEIEAKSSGEGVTSPYSRTEHCENIHEYRCDTSVGVIGIDVSKEHLVTSKGRVRKFTNNEKGYEEILTMKPNIIVIEPTGVYSTRPCQYFKERGVKVLLVSLAMERKGRKGKKTDFYDAEKLENMVNKSREYVNNPLKELVSLYLFMKDVQTKQENRLRRALFLVSDEEKLSEERLEKFSKGDFSDVKLYQLEYTGTVLREIQVLARTLIQTREELKEVRKMIEGNVPQDHVSVSEKFID</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TW0</accession>
    <name>Q97TW0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transposase ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1280</name>
        <name evidence="3" type="ordered locus">SSO1642</name>
        <name evidence="4" type="ordered locus">SSO1904</name>
        <name evidence="5" type="ordered locus">SSO2985</name>
        <name evidence="6" type="ordered locus">SSO3033</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90354">
        <property type="entry name" value="H90354"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923283.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TW0"/>
    <dbReference type="STRING" id="273057.SSO1280"/>
    <dbReference type="PaxDb" id="273057-SSO1280"/>
    <dbReference type="EnsemblBacteria" id="AAK41517">
        <property type="protein sequence ID" value="AAK41517"/>
        <property type="gene ID" value="SSO1280"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41856">
        <property type="protein sequence ID" value="AAK41856"/>
        <property type="gene ID" value="SSO1642"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK42095">
        <property type="protein sequence ID" value="AAK42095"/>
        <property type="gene ID" value="SSO1904"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK43090">
        <property type="protein sequence ID" value="AAK43090"/>
        <property type="gene ID" value="SSO2985"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK43135">
        <property type="protein sequence ID" value="AAK43135"/>
        <property type="gene ID" value="SSO3033"/>
    </dbReference>
    <dbReference type="GeneID" id="1454635"/>
    <dbReference type="KEGG" id="sso:SSO1280"/>
    <dbReference type="KEGG" id="sso:SSO1642"/>
    <dbReference type="KEGG" id="sso:SSO1904"/>
    <dbReference type="KEGG" id="sso:SSO2985"/>
    <dbReference type="KEGG" id="sso:SSO3033"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1279"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="80"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="321" mass="37398" checksum="6101884EE7A53051" modified="2001-10-01" version="1">MQTMILPKTELKSLAITLATNNINVISQDLDPEIVKAAPSLLTGNRGKYYLKVVRRGEKVISKGQRTFKFYPIYREVKGEINVVAVDETGLTVGEKEQEKAEGFLLYNWKRKGVKMRSLDLVYPLRLPLLVEVADLRSDSPSQFLLRSVREVSQYMEIDYVVADAGFLNLGVIKEMPVKTIVRGKSNLKGFKELSNVPLVEKRYEVKDKVYVAYRVLEFEGLYYYDVVYVKGKPRHFMFVTNFEGDPYELAELYRLRWQVEEGFKVRKARIRYVRKLSNKIFLFLYYTVLDSAWNLVNHLLFNFKSTCKKVLSFDSFVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U07</accession>
    <name>Q97U07_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase BadF/BadG/BcrA/BcrD type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90507">
        <property type="entry name" value="E90507"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989688.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U07"/>
    <dbReference type="FunCoup" id="Q97U07">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3218"/>
    <dbReference type="PaxDb" id="273057-SSO3218"/>
    <dbReference type="EnsemblBacteria" id="AAK43316">
        <property type="protein sequence ID" value="AAK43316"/>
        <property type="gene ID" value="SSO3218"/>
    </dbReference>
    <dbReference type="GeneID" id="1453235"/>
    <dbReference type="KEGG" id="sso:SSO3218"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3323"/>
    <dbReference type="eggNOG" id="arCOG03659">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016274_1_0_2"/>
    <dbReference type="InParanoid" id="Q97U07"/>
    <dbReference type="PhylomeDB" id="Q97U07"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045127">
        <property type="term" value="F:N-acetylglucosamine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd24080">
        <property type="entry name" value="ASKHA_NBD_StHK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052519">
        <property type="entry name" value="Euk-type_GlcNAc_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43190">
        <property type="entry name" value="N-ACETYL-D-GLUCOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43190:SF3">
        <property type="entry name" value="N-ACETYL-D-GLUCOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase BadF/BadG/BcrA/BcrD type" evidence="1">
        <location>
            <begin position="4"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="298" mass="32298" checksum="8D95F045010F417F" modified="2001-10-01" version="1">MILVGVDGGGSKTSAIAYTCSGSFLGKGLAGPANFHNVGVEEAVKNVNRAILLATKGMKPDVAYVGLAGVDSRYDYNIMAEALRSIAKNVYVDHDGFVALYAETRGNPGVIVIAGTGSVIVGYDGNRRVRFGGLGWLIADEGSAYWIGREALRAFGKMLDGRINKTIIADKIMKNLNINDVDDLIKWAYHEGHKVKDIASLAKIVDEAANEGDWIALDILKNAAFELASYAVQLAVKIGVDKIFLKGGMFNSSVYYNFFRNYLDNNNIKGVIAEHEPEQGALLLAFKYSECDSSVLNW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2025-02-05" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U12</accession>
    <name>TMCA2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Met) cytidine acetyltransferase TmcA 2</fullName>
            <ecNumber evidence="1 2">2.3.1.193</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tmcA2</name>
        <name evidence="4" type="ordered locus">SSO3214</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2020" name="Nature" volume="583" first="638" last="643">
            <title>Dynamic RNA acetylation revealed by quantitative cross-evolutionary mapping.</title>
            <authorList>
                <person name="Sas-Chen A."/>
                <person name="Thomas J.M."/>
                <person name="Matzov D."/>
                <person name="Taoka M."/>
                <person name="Nance K.D."/>
                <person name="Nir R."/>
                <person name="Bryson K.M."/>
                <person name="Shachar R."/>
                <person name="Liman G.L.S."/>
                <person name="Burkhart B.W."/>
                <person name="Gamage S.T."/>
                <person name="Nobe Y."/>
                <person name="Briney C.A."/>
                <person name="Levy M.J."/>
                <person name="Fuchs R.T."/>
                <person name="Robb G.B."/>
                <person name="Hartmann J."/>
                <person name="Sharma S."/>
                <person name="Lin Q."/>
                <person name="Florens L."/>
                <person name="Washburn M.P."/>
                <person name="Isobe T."/>
                <person name="Santangelo T.J."/>
                <person name="Shalev-Benami M."/>
                <person name="Meier J.L."/>
                <person name="Schwartz S."/>
            </authorList>
            <dbReference type="PubMed" id="32555463"/>
            <dbReference type="DOI" id="10.1038/s41586-020-2418-2"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP).</text>
    </comment>
    <comment type="function">
        <text evidence="2 3">Catalyzes the formation of 41 N(4)-acetylcytidine (ac(4)C) sites in RNA, almost always on the middle C of a CCG motif (PubMed:32555463). Modifications are found mostly in tRNA, with small amounts found in rRNA and mRNA (PubMed:32555463). Four ac(4)C sites (cytidine 1466, 1467, 1477 and 1478) were detected in 16S rRNA, presumably generated by TmcA1 and/or TmcA2 (PubMed:39753558).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>cytidine(34) in elongator tRNA(Met) + acetyl-CoA + ATP + H2O = N(4)-acetylcytidine(34) in elongator tRNA(Met) + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43788"/>
            <dbReference type="Rhea" id="RHEA-COMP:10693"/>
            <dbReference type="Rhea" id="RHEA-COMP:10694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.3.1.193"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a cytidine in RNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in RNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:82211"/>
            <dbReference type="Rhea" id="RHEA-COMP:15704"/>
            <dbReference type="Rhea" id="RHEA-COMP:19834"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a cytidine in tRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in tRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53876"/>
            <dbReference type="Rhea" id="RHEA-COMP:13670"/>
            <dbReference type="Rhea" id="RHEA-COMP:13671"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a cytidine in mRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in mRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:58480"/>
            <dbReference type="Rhea" id="RHEA-COMP:15145"/>
            <dbReference type="Rhea" id="RHEA-COMP:15146"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TmcA family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.193" evidence="1 2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90506">
        <property type="entry name" value="G90506"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992744.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U12"/>
    <dbReference type="SMR" id="Q97U12"/>
    <dbReference type="FunCoup" id="Q97U12">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3214"/>
    <dbReference type="PaxDb" id="273057-SSO3214"/>
    <dbReference type="EnsemblBacteria" id="AAK43310">
        <property type="protein sequence ID" value="AAK43310"/>
        <property type="gene ID" value="SSO3214"/>
    </dbReference>
    <dbReference type="GeneID" id="1453229"/>
    <dbReference type="KEGG" id="sso:SSO3214"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3316"/>
    <dbReference type="eggNOG" id="arCOG01951">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004652_1_0_2"/>
    <dbReference type="InParanoid" id="Q97U12"/>
    <dbReference type="PhylomeDB" id="Q97U12"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990883">
        <property type="term" value="F:18S rRNA cytidine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106162">
        <property type="term" value="F:mRNA N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051392">
        <property type="term" value="F:tRNA cytidine N4-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904812">
        <property type="term" value="P:rRNA acetylation involved in maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051391">
        <property type="term" value="P:tRNA acetylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003957">
        <property type="entry name" value="tRNA(Met) cytidine acetyltransferase TmcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000140">
        <property type="entry name" value="tRNA(Met) cytidine acetyltransferase TmcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01886">
        <property type="entry name" value="tRNA_acetyltr_TmcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007807">
        <property type="entry name" value="TcmA/NAT10_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032672">
        <property type="entry name" value="TmcA/NAT10/Kre33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013562">
        <property type="entry name" value="TmcA/NAT10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024914">
        <property type="entry name" value="tRNA_acetyltr_TmcA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925">
        <property type="entry name" value="N-ACETYLTRANSFERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925:SF5">
        <property type="entry name" value="RNA CYTIDINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13718">
        <property type="entry name" value="GNAT_acetyltr_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05127">
        <property type="entry name" value="NAT10_TcmA_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08351">
        <property type="entry name" value="TmcA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000461794" description="tRNA(Met) cytidine acetyltransferase TmcA 2">
        <location>
            <begin position="1"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="458"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="533"/>
            <end position="535"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2">
        <source>
            <dbReference type="PubMed" id="32555463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="770" mass="87983" checksum="99904FE0985BD162" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U13</accession>
    <name>Q97U13_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome C oxidase assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90506">
        <property type="entry name" value="F90506"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U13"/>
    <dbReference type="PaxDb" id="273057-SSO3213"/>
    <dbReference type="EnsemblBacteria" id="AAK43309">
        <property type="protein sequence ID" value="AAK43309"/>
        <property type="gene ID" value="SSO3213"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3213"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3315"/>
    <dbReference type="eggNOG" id="arCOG03699">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1010574_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U13"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="261" mass="28833" checksum="1C1FCAC2430A347E" modified="2001-10-01" version="1">MIVTYLSALETILAGTTIVFGGIVEGYGYGLSLGTNWPYTNDIMQLAAKKDPEAIHRILATLVGIFSLVILIIHPTLISIIGFVSVVFTALLGMATLYVLAGKLPSVFQGLHDIAAYTTFVSYFLIMLQGLGIFKIDIVSFLISAIVPPHFLYFVIFMGGVVTGTRRMKLKIGRPWERDKERNSWLQAAWTIHGIVALIFIIAVVLLHYWLTLIFTALEIIVGLWVWDSSNRNPLKPGMSIGLHQLFSILVVVAIILNSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U35</accession>
    <name>Q97U35_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">D-3-phosphoglycerate dehydrogenase, putative (SerA-2)</fullName>
            <ecNumber evidence="6">1.1.1.95</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">serA-2</name>
        <name evidence="6" type="ordered locus">SSO3187</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.95" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90503">
        <property type="entry name" value="H90503"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991700.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U35"/>
    <dbReference type="SMR" id="Q97U35"/>
    <dbReference type="FunCoup" id="Q97U35">
        <property type="interactions" value="197"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3187"/>
    <dbReference type="PaxDb" id="273057-SSO3187"/>
    <dbReference type="EnsemblBacteria" id="AAK43287">
        <property type="protein sequence ID" value="AAK43287"/>
        <property type="gene ID" value="SSO3187"/>
    </dbReference>
    <dbReference type="GeneID" id="1453206"/>
    <dbReference type="KEGG" id="sso:SSO3187"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3292"/>
    <dbReference type="eggNOG" id="arCOG01755">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_0_2"/>
    <dbReference type="InParanoid" id="Q97U35"/>
    <dbReference type="PhylomeDB" id="Q97U35"/>
    <dbReference type="BioCyc" id="MetaCyc:MONOMER-18075"/>
    <dbReference type="BRENDA" id="1.1.1.26">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd12165">
        <property type="entry name" value="2-Hacid_dh_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001100">
        <property type="entry name" value="D-isomer specific 2-hydroxyacid dehydrogenase catalytic region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF178">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE YGL185C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1 3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="16"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="93"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="300" mass="33812" checksum="41403FD2CDDF6CD3" modified="2001-10-01" version="1">MKIISTEKVPDECKNIISVKDENITEEDYKNAEILLTWPGRVNNDLIGKMPNLKVIQTFSAGVDDLDFSIIPSHVKVFSNAGAYSLSVAEHAWALILASAKGVGTKKRTIVYDVSEKTLLILGGGGIGSEVARIGKTAFRNYVIGISRSFKKPEWFDERHGMTMLREKIGEADIIVDTLPLNKQTRGLLSYDLLKDIRRNAIIVNVGRGETVDEEGIYKLLKERQDVRFGTDVFWRKNGKEDFYNTKLWELDNFTGTLHTAGAYGNESIMKRAMLIACLNVKKYIDRGIADNEVRREDYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U56</accession>
    <name>Q97U56_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MutT-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO3167</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90501">
        <property type="entry name" value="C90501"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989977.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U56"/>
    <dbReference type="FunCoup" id="Q97U56">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3167"/>
    <dbReference type="PaxDb" id="273057-SSO3167"/>
    <dbReference type="EnsemblBacteria" id="AAK43266">
        <property type="protein sequence ID" value="AAK43266"/>
        <property type="gene ID" value="SSO3167"/>
    </dbReference>
    <dbReference type="GeneID" id="1453185"/>
    <dbReference type="KEGG" id="sso:SSO3167"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3271"/>
    <dbReference type="eggNOG" id="arCOG01072">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040940_5_3_2"/>
    <dbReference type="InParanoid" id="Q97U56"/>
    <dbReference type="PhylomeDB" id="Q97U56"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03426">
        <property type="entry name" value="NUDIX_CoAse_Nudt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992:SF11">
        <property type="entry name" value="MITOCHONDRIAL COENZYME A DIPHOSPHATASE NUDT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="7">
        <location>
            <begin position="16"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK43266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="159" mass="18198" checksum="B3D48848BB64678F" modified="2001-10-01" version="1">MNISKLLQLPLTDDNESDAAVVVLIAKGQYILLIKRVSNPKDPWSGQMALPGGHRENNETAFQAAIRECEEEVGIRPNIRSSLGVFSPNNIKIKVRAYIALLDELIEPRPNPVEVDKVFWVHESELVRGDNAFYYKQYRIWGMTYRILSKLFEIAELKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UI2</accession>
    <name>Q97UI2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dihydrodipicolinate synthase (DapA-2)</fullName>
            <ecNumber evidence="3">4.3.3.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dapA-2</name>
        <name evidence="3" type="ordered locus">SSO3035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.3.3.7" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90485">
        <property type="entry name" value="B90485"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990702.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UI2"/>
    <dbReference type="FunCoup" id="Q97UI2">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3035"/>
    <dbReference type="PaxDb" id="273057-SSO3035"/>
    <dbReference type="EnsemblBacteria" id="AAK43137">
        <property type="protein sequence ID" value="AAK43137"/>
        <property type="gene ID" value="SSO3035"/>
    </dbReference>
    <dbReference type="GeneID" id="1453056"/>
    <dbReference type="KEGG" id="sso:SSO3035"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3134"/>
    <dbReference type="eggNOG" id="arCOG04172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_5_0_2"/>
    <dbReference type="InParanoid" id="Q97UI2"/>
    <dbReference type="PhylomeDB" id="Q97UI2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008675">
        <property type="term" value="F:2-dehydro-3-deoxy-phosphogluconate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00408">
        <property type="entry name" value="DHDPS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128:SF66">
        <property type="entry name" value="4-HYDROXY-2-OXOGLUTARATE ALDOLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="DIHYDRODIPICOLINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="2">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="302" mass="34047" checksum="710E674A835D61B2" modified="2001-10-01" version="1">MDGVFLTNVTPFTKTLELDLEGLKRIVEFAQNSGYKYIVPLGTAGEFSSLTVEEKVRVVDHVRRALSKGEIIAGASSTSFLETGELCKKYKEIGVDKIMVLPPYYLKGNEKGLMEYFFKVAELCDSTMVLYNNPSSTGVDLSPQLIKRLNDMIPSFRVLKEARYNVVEFKEVVRLVGDKIDVIDGLEERALLGLVLGAKGFTTSMGSFSSLPLMIYESYEKKRNIDTAIRLYDLLLEYRSFIKLPLTMSHLAKLGAWLRGLISSYATRPPLPNLDDVVSEDVKRKMIEKINQIISFEKNTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UI5</accession>
    <name>Q97UI5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90484">
        <property type="entry name" value="F90484"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924100.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UI5"/>
    <dbReference type="STRING" id="273057.SSO3031"/>
    <dbReference type="PaxDb" id="273057-SSO3031"/>
    <dbReference type="EnsemblBacteria" id="AAK43133">
        <property type="protein sequence ID" value="AAK43133"/>
        <property type="gene ID" value="SSO3031"/>
    </dbReference>
    <dbReference type="GeneID" id="1453052"/>
    <dbReference type="KEGG" id="sso:SSO3031"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3127"/>
    <dbReference type="eggNOG" id="arCOG06508">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134214_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UI5"/>
    <dbReference type="PhylomeDB" id="Q97UI5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase InsH N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="140" mass="16408" checksum="548F1135E2AFAB4B" modified="2001-10-01" version="1">MVKAISTEKDLLLKIDKSFPWETFRSKLKSLYSKKPKWDVILLLKVLLIKFVFDISWNNLEGEIRDSKSFMDFLGGKVPPKSTVFFFYKKLQQTVIQEGETMRTTLMDELNKALNKVISEYREFELEVGREKTIGSRTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UJ8</accession>
    <name>Q97UJ8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dehydrogenase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO3013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90482">
        <property type="entry name" value="D90482"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992668.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UJ8"/>
    <dbReference type="FunCoup" id="Q97UJ8">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3013"/>
    <dbReference type="PaxDb" id="273057-SSO3013"/>
    <dbReference type="EnsemblBacteria" id="AAK43115">
        <property type="protein sequence ID" value="AAK43115"/>
        <property type="gene ID" value="SSO3013"/>
    </dbReference>
    <dbReference type="GeneID" id="1453034"/>
    <dbReference type="KEGG" id="sso:SSO3013"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3105"/>
    <dbReference type="eggNOG" id="arCOG01622">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_1_4_2"/>
    <dbReference type="InParanoid" id="Q97UJ8"/>
    <dbReference type="PhylomeDB" id="Q97UJ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051450">
        <property type="entry name" value="Gfo/Idh/MocA_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377:SF1">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="2">
        <location>
            <begin position="127"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="319" mass="36095" checksum="1A17F81241F16768" modified="2001-10-01" version="1">MKIAVVGCDGFGKVHLRAMRNISGIEYYVFSRNEEKARECMKEFNAEGYFTRYEDVIKSNVDIVDLLVSHDQHYPMGVMAMKAGKHLMLEKPIARTIEEAMGLINTSKDTKRKFMVLEQFYFESSVRKAKELLPRLGNLSLIIVRSIHLYQPKGWRREKEKMGGGALIDGGVHFIDTLLNLGGEYESVRGVCGKYFSGIEGEDLTLATLRFRKGHLGLLMYSWTTPNPPKVPAFEIYGENGSIIEDPNTRVMGKAYGDLILNVDGKEERIEVEKVNPIEEEIRGFVKAVKNNTEVPMPPEIALRDLKAVLDIYKSCGYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97US0</accession>
    <name>Q97US0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1234</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2928</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90472">
        <property type="entry name" value="C90472"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97US0"/>
    <dbReference type="STRING" id="273057.SSO2928"/>
    <dbReference type="PaxDb" id="273057-SSO2928"/>
    <dbReference type="EnsemblBacteria" id="AAK43034">
        <property type="protein sequence ID" value="AAK43034"/>
        <property type="gene ID" value="SSO2928"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2928"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075479_2_1_2"/>
    <dbReference type="InParanoid" id="Q97US0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="99" mass="11370" checksum="9F07C4279B941129" modified="2001-10-01" version="1">MGVEGSTMARTLRHIPNLMYYPLPPIEDMPWREKWLTEIKPVLDAMDLERVLGEGALVYLKLLIVMVLYSCSYRDAVKMVNVSAFRKFIALGKGYTTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UT9</accession>
    <name>Q97UT9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lycopene cyclase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO2904</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Carotenoid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90469">
        <property type="entry name" value="F90469"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924028.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UT9"/>
    <dbReference type="STRING" id="273057.SSO2904"/>
    <dbReference type="PaxDb" id="273057-SSO2904"/>
    <dbReference type="EnsemblBacteria" id="AAK43013">
        <property type="protein sequence ID" value="AAK43013"/>
        <property type="gene ID" value="SSO2904"/>
    </dbReference>
    <dbReference type="GeneID" id="1452932"/>
    <dbReference type="KEGG" id="sso:SSO2904"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2994"/>
    <dbReference type="eggNOG" id="arCOG05416">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083013_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UT9"/>
    <dbReference type="BRENDA" id="5.5.1.19">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016872">
        <property type="term" value="F:intramolecular lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045436">
        <property type="term" value="F:lycopene beta cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017825">
        <property type="entry name" value="Lycopene_cyclase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03462">
        <property type="entry name" value="CarR_dom_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18916">
        <property type="entry name" value="Lycopene_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Lycopene cyclase" evidence="9">
        <location>
            <begin position="8"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Lycopene cyclase" evidence="9">
        <location>
            <begin position="126"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK43013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="226" mass="26664" checksum="C0A453C5634F9381" modified="2001-10-01" version="1">MEIFKPDYLMIDSMIFFPTLILSFLIKRNYVKLLKSIGIVSPIYLFWDFLATWRNSWTFNPKYVAGIYVIDLPIEEVMFFLVTPFATLLIFDFVMGKVRDKEVKWVPKVVCVAIPLLLLTLPFVYNYSYTFIDLVYLIMSLIVSLLIGRNLLASRNFWIFMGLSYIPFLVFDYFLTSDPVVIYGSHSIVGIRFITIPIEDFIYSFSMITFYTVFYIRGNSLWMKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UU0</accession>
    <name>Q97UU0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2903</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90469">
        <property type="entry name" value="E90469"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924027.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UU0"/>
    <dbReference type="STRING" id="273057.SSO2903"/>
    <dbReference type="PaxDb" id="273057-SSO2903"/>
    <dbReference type="EnsemblBacteria" id="AAK43012">
        <property type="protein sequence ID" value="AAK43012"/>
        <property type="gene ID" value="SSO2903"/>
    </dbReference>
    <dbReference type="GeneID" id="1452931"/>
    <dbReference type="KEGG" id="sso:SSO2903"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2993"/>
    <dbReference type="eggNOG" id="arCOG07726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1451472_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UU0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="186" mass="21467" checksum="2737F5E206A1DF44" modified="2001-10-01" version="1">MDETEIEKLYNGKLDDLYYLYSHANSEDIIRWMKNRKTAEMRTYEVEGDSEIVVVIPTADVNGKLARNVREVYKGFHIIFIESFGSLFNYARSVNFGLKSSLRLKPRWVIISNDDVLSVSGNIKDELSIVSRNVNLVMASRSNYHTYPVVLVKPNEYFIRGMKIFGKVLNFSPAEVYGEILSHKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UW8</accession>
    <name>Q97UW8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl-CoA c-acetyltransferase (Acetoacetyl-CoA thiolase) (AcaB-9)</fullName>
            <ecNumber evidence="4">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">acaB-9</name>
        <name evidence="4" type="ordered locus">SSO2874</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.9" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90465">
        <property type="entry name" value="E90465"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989826.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UW8"/>
    <dbReference type="FunCoup" id="Q97UW8">
        <property type="interactions" value="319"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2874"/>
    <dbReference type="PaxDb" id="273057-SSO2874"/>
    <dbReference type="EnsemblBacteria" id="AAK42980">
        <property type="protein sequence ID" value="AAK42980"/>
        <property type="gene ID" value="SSO2874"/>
    </dbReference>
    <dbReference type="GeneID" id="15298822"/>
    <dbReference type="KEGG" id="sso:SSO2874"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2959"/>
    <dbReference type="eggNOG" id="arCOG01278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_2"/>
    <dbReference type="InParanoid" id="Q97UW8"/>
    <dbReference type="PhylomeDB" id="Q97UW8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004720">
        <property type="entry name" value="PRK06064.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF6">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="3">
        <location>
            <begin position="239"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="395" mass="43201" checksum="5347B3CC682339C3" modified="2001-10-01" version="1">MRNVAIIGTGHTKFGVRTDVNLQELAWEAVKQALEEANIDQNEIQYFVVGNVGNWSSEELPAVIVGEYCNLTPKGTMRVEAACSTGSAAIRDAYLAIKSGEADIALVVGVEQMHQAPNPQVVELIGRAGGYFWEFENFGLTFPGYYALHASAYMAKYGMKEEDLGKIAIKNHYYGAKNPYAQFQKEITMEEYLKSKPVAYPLKLLDSSPITDGAAAIVLASEEVAKKITDSPVWIVSQGVASGTANLSRRTDFTHIEAAHIAAQQAYKRARIEFDNAWKNFDVADVHDCFTIAEIMAYEDLGFAKRGEGYLLAREEQTYIGGKIPVNVDGGLKAKGHPIGATGVSMAVSITRQLLYRAHKGTQVEVRNGMGITHNVGGTGHYAYVTIYSTRRPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UZ2</accession>
    <name>Q97UZ2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2846</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90462">
        <property type="entry name" value="E90462"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923997.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UZ2"/>
    <dbReference type="STRING" id="273057.SSO2846"/>
    <dbReference type="PaxDb" id="273057-SSO2846"/>
    <dbReference type="EnsemblBacteria" id="AAK42956">
        <property type="protein sequence ID" value="AAK42956"/>
        <property type="gene ID" value="SSO2846"/>
    </dbReference>
    <dbReference type="GeneID" id="1452875"/>
    <dbReference type="KEGG" id="sso:SSO2846"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2931"/>
    <dbReference type="eggNOG" id="arCOG01021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2079533_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UZ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="117" mass="12421" checksum="BBD266906DE94255" modified="2001-10-01" version="1">MEGKYKRAISTSTAIIIAVVVIILIVVGVVAYFQQMGSHAPTSSSSMTSQSSVISTSQSQTTTQQVIKIGALLPLTGQLSSVGAEAQQVLMYAQDQANALLKQLGVNTVVFHQFYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UZ9</accession>
    <name>Q97UZ9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter, permease (Branched chain amino acid)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO2839</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90461">
        <property type="entry name" value="E90461"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923992.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UZ9"/>
    <dbReference type="FunCoup" id="Q97UZ9">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2839"/>
    <dbReference type="PaxDb" id="273057-SSO2839"/>
    <dbReference type="EnsemblBacteria" id="AAK42948">
        <property type="protein sequence ID" value="AAK42948"/>
        <property type="gene ID" value="SSO2839"/>
    </dbReference>
    <dbReference type="GeneID" id="1452867"/>
    <dbReference type="KEGG" id="sso:SSO2839"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2924"/>
    <dbReference type="eggNOG" id="arCOG01269">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_1_0_2"/>
    <dbReference type="InParanoid" id="Q97UZ9"/>
    <dbReference type="PhylomeDB" id="Q97UZ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06582">
        <property type="entry name" value="TM_PBP1_LivH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052157">
        <property type="entry name" value="BCAA_transport_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11795:SF449">
        <property type="entry name" value="BRANCHED-CHAIN AMINO ACID TRANSPORT PERMEASE PROTEIN LIVH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11795">
        <property type="entry name" value="BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM PERMEASE PROTEIN LIVH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="224"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="305" mass="32716" checksum="23F29C72DB004DB9" modified="2001-10-01" version="1">MIDPLIINTIVYGSILGLSSLSLTLTYLTTKVPNFAHGIFLLFGSYMTFTIAEVLKQNPYISLPIAFLLGGAIALLQYYGVLLPLSMRGGGLVSQMVATLAFSILLYGGLNAYASYLGYVYNVQSRDVTLGFLDYEIGGIRAVFLISIILAIAFILSLYIFLNKTRLGISIRATVENSSLAESMGINTKLVKAISWFLAGGIASTAGSLYPLQFVFSPTLAYSILLSIFAASVLGGLMSLFGGFIGGFIEGFIEKYIMTELSIYLSPLIGVSPFVINQYSTLVPLIVVAAVLLIAPNGIMGVRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V53</accession>
    <name>Q97V53_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2782</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90454">
        <property type="entry name" value="E90454"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V53"/>
    <dbReference type="STRING" id="273057.SSO2782"/>
    <dbReference type="PaxDb" id="273057-SSO2782"/>
    <dbReference type="EnsemblBacteria" id="AAK42892">
        <property type="protein sequence ID" value="AAK42892"/>
        <property type="gene ID" value="SSO2782"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2782"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2864"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_2_2"/>
    <dbReference type="InParanoid" id="Q97V53"/>
    <dbReference type="PhylomeDB" id="Q97V53"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="151"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="276"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="374" mass="44200" checksum="E64F6511FC0E405E" modified="2001-10-01" version="1">MKLACEIYNTLRWADIYFYQRDGKGLTQTELRQLALDLRKQDDEYKQVYSQVVQQIADRYYEARQRFFEGLARFPKEKKPHKYYSLVYPQYGWKILQVREIRKGKKKLITLKLSNLGTFKVIVHRDFPLDKVKRVVVKLTRSERIYITFVVDHEFPKLPNTGKVVAIDVGIEKLIVTSDGEYFPNLRPYEKALWKVKHLHRELSRKKFLSNNWFKAKVKLARAYEYLKNLRTDLYMKLGKWFAEHYDVVVMEDIHVKQLIGKSLRSLRRRLSDVAFSELRDLIKYQLEKYGKKLILVNPAYTSKTCARCGYVKEDLSLSDRVFVCSNCGWIADRDYNASLNILKGAGSERSLVPVELRPLPVPALRYWHGRAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V62</accession>
    <name>Q97V62_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="4">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ilvB-4</name>
        <name evidence="11" type="ordered locus">SSO2774</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90453">
        <property type="entry name" value="D90453"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990225.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V62"/>
    <dbReference type="STRING" id="273057.SSO2774"/>
    <dbReference type="PaxDb" id="273057-SSO2774"/>
    <dbReference type="EnsemblBacteria" id="AAK42883">
        <property type="protein sequence ID" value="AAK42883"/>
        <property type="gene ID" value="SSO2774"/>
    </dbReference>
    <dbReference type="GeneID" id="1452802"/>
    <dbReference type="KEGG" id="sso:SSO2774"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2855"/>
    <dbReference type="eggNOG" id="arCOG01998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_1_2"/>
    <dbReference type="InParanoid" id="Q97V62"/>
    <dbReference type="PhylomeDB" id="Q97V62"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00568">
        <property type="entry name" value="TPP_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="10">
        <location>
            <begin position="4"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="8">
        <location>
            <begin position="197"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="9">
        <location>
            <begin position="369"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK42883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="531" mass="59516" checksum="96C406CD7C1C913E" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V72</accession>
    <name>Q97V72_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Electron transfer flavoprotein, subunit alpha (EtfA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">etfA</name>
        <name evidence="5" type="ordered locus">SSO2762</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90452">
        <property type="entry name" value="B90452"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990243.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V72"/>
    <dbReference type="FunCoup" id="Q97V72">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2762"/>
    <dbReference type="PaxDb" id="273057-SSO2762"/>
    <dbReference type="EnsemblBacteria" id="AAK42873">
        <property type="protein sequence ID" value="AAK42873"/>
        <property type="gene ID" value="SSO2762"/>
    </dbReference>
    <dbReference type="GeneID" id="1452792"/>
    <dbReference type="KEGG" id="sso:SSO2762"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2843"/>
    <dbReference type="eggNOG" id="arCOG00447">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034178_0_1_2"/>
    <dbReference type="InParanoid" id="Q97V72"/>
    <dbReference type="PhylomeDB" id="Q97V72"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000004">
        <property type="entry name" value="Electron transfer flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000300">
        <property type="entry name" value="Electron transfer flavoprotein alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF11">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN, SUBUNIT ALPHA (ETFA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="287" mass="30621" checksum="7C22AC44ED4D9EDD" modified="2001-10-01" version="1">MKTVVFSESPVFLRMAAAVAQSFGGEVIGISDNENVKFVNKLYIIDKIDEDGIVDLISSLSPDVILTGNVRRDRAIAGRVAGRLKLPIATDVVSLKVEDKKVTLNRVAYSGMGLVTLESELPIVITIANTQLQPKELQPSVEKVQLKEGRVKIVGRKSTSSGVNLATADIVVGVGRGIGSKENVKYAEELAKALGGAVGGSRPVAAELNWVSEDRQIGLSGLRIRPKLYIALGISGQPQHIAGIRDSKIIVAVNIDKNAPILENADYLVVGNAIDFCKAMLSKLGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V79</accession>
    <name>Q97V79_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="3">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">porB-2</name>
        <name evidence="7" type="ordered locus">SSO2756</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90451">
        <property type="entry name" value="C90451"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990254.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V79"/>
    <dbReference type="FunCoup" id="Q97V79">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2756"/>
    <dbReference type="PaxDb" id="273057-SSO2756"/>
    <dbReference type="EnsemblBacteria" id="AAK42866">
        <property type="protein sequence ID" value="AAK42866"/>
        <property type="gene ID" value="SSO2756"/>
    </dbReference>
    <dbReference type="GeneID" id="1452785"/>
    <dbReference type="KEGG" id="sso:SSO2756"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2835"/>
    <dbReference type="eggNOG" id="arCOG01601">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058423_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V79"/>
    <dbReference type="PhylomeDB" id="Q97V79"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03376">
        <property type="entry name" value="TPP_PFOR_porB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051479">
        <property type="entry name" value="PorB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008818">
        <property type="entry name" value="PRK11864.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008819">
        <property type="entry name" value="PRK11865.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42897:SF1">
        <property type="entry name" value="2-OXOACID OXIDOREDUCTASE (FERREDOXIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42897">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="6">
        <location>
            <begin position="49"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="299" mass="32788" checksum="C16E806155C0D0AE" modified="2001-10-01" version="1">MSLGLREILNKHNGILSGTSACPGCPENMAMRMLGMGLGKDVVLVVVAGCSSVIQGNAPYNAYNFPTVNVAFAAGPATAAGLARAYKQKGKNVTIVVWAGDGGSADIGFASLSGAAERNDNILYLTVDNEAYMNSGGQRSGSTPFGAITTTTPEGKKENKKNLPLLMIAHNVPYVATASVGYPHDYIEKLKKARQVEGFRYVHVLTPDPYGWLFDPSKTVEVAKLAVQTCYWPLFEYYNGKLNISSESLHCLDRRTRRPIRDFLSIQGRFKKLTDEQIKILEQYIDNLWEKLKSMLNNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VA4</accession>
    <name>Q97VA4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90448">
        <property type="entry name" value="B90448"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991728.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VA4"/>
    <dbReference type="PaxDb" id="273057-SSO2730"/>
    <dbReference type="EnsemblBacteria" id="AAK42841">
        <property type="protein sequence ID" value="AAK42841"/>
        <property type="gene ID" value="SSO2730"/>
    </dbReference>
    <dbReference type="GeneID" id="1452760"/>
    <dbReference type="KEGG" id="sso:SSO2730"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2810"/>
    <dbReference type="eggNOG" id="arCOG03169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061108_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VA4"/>
    <dbReference type="PhylomeDB" id="Q97VA4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301:SF8">
        <property type="entry name" value="ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301">
        <property type="entry name" value="DNA-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="14"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="377" mass="42924" checksum="560BDA95FE270337" modified="2001-10-01" version="1">MWFIYGRPTNNPFGREKEIESLLGLYKLGQPVGLIGPRRIGKTSLLLASLEHSSFPYSLISAEEFVRGEKGFDFAEFLSAYITSVTSTIYSKANYKIILEKGKSYLKQLRDLLGQVKVTFNIPELYSTIELTLDKAEKKKYLEKEFRDALDLPQILSEKFGLQRVVIAIDEFQYLRLAKQSIPGIFHIMRSKWQFHKNVTYVISGSLIGMMMELLSSRNQPFYQFFYLMKVNPFDRKTSIEFLRKGFEVNNLDVKSEEIELIVDQVDGFPAWLNLVGIKIVTEKRSANEVINSLVKDINLVTALENDLKRLSPTARAVLKNLASLGGAGSPKDLGLDLLTVTRGLKQLMNYGLVEKEGRGIYRIVDPILIKYLVSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VB4</accession>
    <name>Q97VB4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90446">
        <property type="entry name" value="H90446"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VB4"/>
    <dbReference type="STRING" id="273057.SSO2721"/>
    <dbReference type="PaxDb" id="273057-SSO2721"/>
    <dbReference type="EnsemblBacteria" id="AAK42831">
        <property type="protein sequence ID" value="AAK42831"/>
        <property type="gene ID" value="SSO2721"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2721"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2802"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113529_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VB4"/>
    <dbReference type="PhylomeDB" id="Q97VB4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025471">
        <property type="entry name" value="DUF4322"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14210">
        <property type="entry name" value="DUF4322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4322" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="164" mass="18507" checksum="A342C639F43F3202" modified="2001-10-01" version="1">MLNFKGKKAEEVARVLVSACLWNDSVEGRSKGYNVSPQTVRNYVSILEKFLDYDLVVYPLSKAINFLKALVKEQGNGVIEKLLESMRRISMEMLKGVKEVDISIDWTTKTWYGKPVKGLGSSAKGNSYNYATATTKYKGMVLFLVFVPQVNGMTKDEIVKFLME</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VE0</accession>
    <name>Q97VE0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mercury resistance operon repressor, probable (MerR)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">merR</name>
        <name evidence="5" type="ordered locus">SSO2688</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90443">
        <property type="entry name" value="E90443"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988609.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VE0"/>
    <dbReference type="STRING" id="273057.SSO2688"/>
    <dbReference type="PaxDb" id="273057-SSO2688"/>
    <dbReference type="EnsemblBacteria" id="AAK42804">
        <property type="protein sequence ID" value="AAK42804"/>
        <property type="gene ID" value="SSO2688"/>
    </dbReference>
    <dbReference type="GeneID" id="1452723"/>
    <dbReference type="KEGG" id="sso:SSO2688"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2769"/>
    <dbReference type="eggNOG" id="arCOG01681">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_5_3_2"/>
    <dbReference type="InParanoid" id="Q97VE0"/>
    <dbReference type="PhylomeDB" id="Q97VE0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051081">
        <property type="entry name" value="HTH_MetalResp_TranReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033788">
        <property type="entry name" value="HTH_metalloreg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154:SF36">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ARSR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="115" mass="12806" checksum="FA681474952DF5DA" modified="2001-10-01" version="1">MEPLTNELESLFSALADGTRLRIVLFLLDKGQATVDEISKSLGKSQSLISHHMACLRNCGIVKVRKDGKFSYYSISTPEIIELIKLSINHVKKYSQSILSCDVLAEEKGQVKLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VF2</accession>
    <name>Q97VF2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyl-hydrolase family 116 catalytic region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2674</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99442">
        <property type="entry name" value="A99442"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923952.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VF2"/>
    <dbReference type="FunCoup" id="Q97VF2">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2674"/>
    <dbReference type="CAZy" id="GH116">
        <property type="family name" value="Glycoside Hydrolase Family 116"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2674"/>
    <dbReference type="DNASU" id="1452711"/>
    <dbReference type="EnsemblBacteria" id="AAK42792">
        <property type="protein sequence ID" value="AAK42792"/>
        <property type="gene ID" value="SSO2674"/>
    </dbReference>
    <dbReference type="GeneID" id="1452711"/>
    <dbReference type="KEGG" id="sso:SSO2674"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2756"/>
    <dbReference type="eggNOG" id="arCOG03863">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027051_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VF2"/>
    <dbReference type="PhylomeDB" id="Q97VF2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000094">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006775">
        <property type="entry name" value="GH116_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052566">
        <property type="entry name" value="Non-lysos_glucosylceramidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654">
        <property type="entry name" value="BILE ACID BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654:SF0">
        <property type="entry name" value="NON-LYSOSOMAL GLUCOSYLCERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04685">
        <property type="entry name" value="DUF608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl-hydrolase family 116 catalytic region" evidence="1">
        <location>
            <begin position="305"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="648" mass="74597" checksum="4CE06BD8F9F9C2CE" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VF9</accession>
    <name>Q97VF9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2667</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90441">
        <property type="entry name" value="B90441"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VF9"/>
    <dbReference type="STRING" id="273057.SSO2667"/>
    <dbReference type="PaxDb" id="273057-SSO2667"/>
    <dbReference type="EnsemblBacteria" id="AAK42785">
        <property type="protein sequence ID" value="AAK42785"/>
        <property type="gene ID" value="SSO2667"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2667"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2749"/>
    <dbReference type="eggNOG" id="arCOG03824">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1352160_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VF9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="202" mass="23604" checksum="D5298B190984AE5A" modified="2001-10-01" version="1">MVLGYWLFIFSNYTICWTYTSWLRGSVKREAKEMKLLPFYMIGNNIFIDAHFLSSISKPYIRILLVRSNLKLEQEGEKLEPIPLDHVIKLLEDGYVDLKSLSEKKERLDSLDYLLSSLYNPGWYFRKREAEITSSSLLRIYDLAYKLVLKKLRDLSRVDVVFVSISFSNDSILINGKEDRIYTTLFKSDSNFKKAILNSLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VR1</accession>
    <name>Q97VR1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90428">
        <property type="entry name" value="B90428"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990124.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VR1"/>
    <dbReference type="FunCoup" id="Q97VR1">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2552"/>
    <dbReference type="PaxDb" id="273057-SSO2552"/>
    <dbReference type="EnsemblBacteria" id="AAK42681">
        <property type="protein sequence ID" value="AAK42681"/>
        <property type="gene ID" value="SSO2552"/>
    </dbReference>
    <dbReference type="GeneID" id="1454008"/>
    <dbReference type="KEGG" id="sso:SSO2552"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2634"/>
    <dbReference type="eggNOG" id="arCOG05967">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_432549_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VR1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021102">
        <property type="entry name" value="PNGase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056948">
        <property type="entry name" value="PNGaseA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31104">
        <property type="entry name" value="PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12222">
        <property type="entry name" value="PNGaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal" evidence="1">
        <location>
            <begin position="77"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="610" mass="68640" checksum="CFD8BFC0D2EC4FC2" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VT1</accession>
    <name>Q97VT1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2529</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90425">
        <property type="entry name" value="D90425"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990974.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VT1"/>
    <dbReference type="STRING" id="273057.SSO2529"/>
    <dbReference type="PaxDb" id="273057-SSO2529"/>
    <dbReference type="EnsemblBacteria" id="AAK42659">
        <property type="protein sequence ID" value="AAK42659"/>
        <property type="gene ID" value="SSO2529"/>
    </dbReference>
    <dbReference type="GeneID" id="1453986"/>
    <dbReference type="KEGG" id="sso:SSO2529"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2606"/>
    <dbReference type="eggNOG" id="arCOG01197">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130257_9_4_2"/>
    <dbReference type="InParanoid" id="Q97VT1"/>
    <dbReference type="PhylomeDB" id="Q97VT1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43449">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43449:SF1">
        <property type="entry name" value="POLYMERASE BETA NUCLEOTIDYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="24"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="122" mass="14116" checksum="3686B8F39D0DCF03" modified="2001-10-01" version="1">MGKAKSALKSQVELVKVAIEFINDISNEIRIEDVYVVGSRARGDYLETSDIDLVIISRDFEGLRYIDRLEKLGKHLRARVEFFAFTPEEWKEPKSLLIKEMKKEAKRLEDLAKEIGLSFLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VV4</accession>
    <name>Q97VV4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3211 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2502</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90422">
        <property type="entry name" value="E90422"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923904.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VV4"/>
    <dbReference type="STRING" id="273057.SSO2502"/>
    <dbReference type="PaxDb" id="273057-SSO2502"/>
    <dbReference type="EnsemblBacteria" id="AAK42636">
        <property type="protein sequence ID" value="AAK42636"/>
        <property type="gene ID" value="SSO2502"/>
    </dbReference>
    <dbReference type="GeneID" id="1453963"/>
    <dbReference type="KEGG" id="sso:SSO2502"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2581"/>
    <dbReference type="eggNOG" id="arCOG07265">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154341_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VV4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021578">
        <property type="entry name" value="STK_08120-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11485">
        <property type="entry name" value="STK_08120-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15309" checksum="49A2785507C2E951" modified="2001-10-01" version="1">MEITFSTQHNIQALAKILSDPIFTLYKILGAETVSVNGNEFDALISLGVTSLILHGTVYVGNNKVSYKFYSVGTQKGEGGVLEFDLFKEGEIRLIVEFEGRFASFIKFSLRRKIEKNIKRFDEEVRLERIKRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VV5</accession>
    <name>Q97VV5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alcohol dehydrogenase (Zn containing) (Adh-9)</fullName>
            <ecNumber evidence="8">1.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">adh-9</name>
        <name evidence="8" type="ordered locus">SSO2501</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90422">
        <property type="entry name" value="D90422"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923903.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VV5"/>
    <dbReference type="FunCoup" id="Q97VV5">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2501"/>
    <dbReference type="PaxDb" id="273057-SSO2501"/>
    <dbReference type="EnsemblBacteria" id="AAK42635">
        <property type="protein sequence ID" value="AAK42635"/>
        <property type="gene ID" value="SSO2501"/>
    </dbReference>
    <dbReference type="GeneID" id="1453962"/>
    <dbReference type="KEGG" id="sso:SSO2501"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2580"/>
    <dbReference type="eggNOG" id="arCOG01455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_2_2"/>
    <dbReference type="InParanoid" id="Q97VV5"/>
    <dbReference type="PhylomeDB" id="Q97VV5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08263">
        <property type="entry name" value="Zn_ADH10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="10"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="348" mass="36921" checksum="18156A63A96D260E" modified="2001-10-01" version="1">MKAAVYKGYGLPLEIEEVPNPAPKEGEVLVKVASTGICHSDLHLLSGELVGPLPNGFIIGHEIAGWVEKIGENVKNPYGLKEGDPVLVSWIVPCGICRYCANGKENYCKDNAKRLVGLIGINGGHAEYLTVPEIAVIPLAKNLDPYYSSPIACAYGTAYNALKSANATSGKSVVIVGAGGVGSAAIQLANSIGLNPIVAVDIDENKLKSAKLLGATHIINANENDARAKVLEALEDGADIVYETKPYPDFKLSLEVVKSGGTIVVTGLGGFSTLAQIPVTLFVSRGITLIGSLGYRPRIDLPDLVSLASSGKIDIKKLVSHIYTPDMINEAYENLKKGLHIRAIIRWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VW9</accession>
    <name>Q97VW9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyl transferase family 28 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2485</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90420">
        <property type="entry name" value="F90420"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923889.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VW9"/>
    <dbReference type="STRING" id="273057.SSO2485"/>
    <dbReference type="PaxDb" id="273057-SSO2485"/>
    <dbReference type="EnsemblBacteria" id="AAK42621">
        <property type="protein sequence ID" value="AAK42621"/>
        <property type="gene ID" value="SSO2485"/>
    </dbReference>
    <dbReference type="GeneID" id="7808240"/>
    <dbReference type="KEGG" id="sso:SSO2485"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2566"/>
    <dbReference type="eggNOG" id="arCOG01394">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_873241_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VW9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21015:SF22">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21015">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--N-ACETYLMURAMYL-(PENTAPEPTIDE) PYROPHOSPHORYL-UNDECAPRENOL N-ACETYLGLUCOSAMINE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl transferase family 28 C-terminal" evidence="2">
        <location>
            <begin position="169"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="318" mass="35703" checksum="BE99703FCCE2C70E" modified="2001-10-01" version="1">MTRILIIASGGGHTGFARAIAQYLPEKVDFVIPTNDPYSRQMISQYANKIYEIPKGREPDESSLVLFKRLFSIIVKSASIKKYDLIIATGSNHSIFPSFFQFLKGGKVYGIESQDRLITKGKAIGIISNYAKGVFLHWQEQRKLYEKKGIVVGPIVEKPKYESKDEGYILVTTGSMGFKRLFDAIVKSVIGKRIVIQTGKIDPTIYRNQNVTTFSFDPDLEKWIANASLVITHQGKTAMEAVVMYRKPVIMVYNNDWKSATTLSDAKKYAEILGATFLSDPITWSDYNVLLEAIENANEPNVFDTGTPNLVKHVLSEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2025-06-18" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VY2</accession>
    <name>LEUC_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-isopropylmalate dehydratase large subunit</fullName>
            <ecNumber evidence="1">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alpha-IPM isomerase</fullName>
            <shortName evidence="1">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuC</name>
        <name type="ordered locus">SSO2471</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99419">
        <property type="entry name" value="A99419"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VY2"/>
    <dbReference type="SMR" id="Q97VY2"/>
    <dbReference type="FunCoup" id="Q97VY2">
        <property type="interactions" value="209"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2471"/>
    <dbReference type="PaxDb" id="273057-SSO2471"/>
    <dbReference type="EnsemblBacteria" id="AAK42608">
        <property type="protein sequence ID" value="AAK42608"/>
        <property type="gene ID" value="SSO2471"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2471"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2548"/>
    <dbReference type="eggNOG" id="arCOG01698">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_3_4_2"/>
    <dbReference type="InParanoid" id="Q97VY2"/>
    <dbReference type="PhylomeDB" id="Q97VY2"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01027">
        <property type="entry name" value="LeuC_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011826">
        <property type="entry name" value="HAcnase/IPMdehydase_lsu_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006251">
        <property type="entry name" value="Homoacnase/IPMdehydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01343">
        <property type="entry name" value="hacA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02086">
        <property type="entry name" value="IPMI_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001614">
        <property type="entry name" value="PRK00402.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF2">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000076885" description="3-isopropylmalate dehydratase large subunit">
        <location>
            <begin position="1"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01027"/>
        </source>
    </evidence>
    <sequence length="416" mass="45253" checksum="5A342E12C6B88796" modified="2001-10-01" version="1">MMSAKTLTEKILSRASGKDVSPGDVIEAKVDLVAFHDLTGYHVIEVMEKANMIKVFDKSKLVIAFDHLAPPPDVRSAEIQGYIRKFVKSEGIPNFYDINYGILHEVMIEQYANPGQVILAADSHTTTSGAVGAFAQGMGASDIAAALITGKTWLVVPQPFKVVLEGKPAKWITGKDVALKLLGDFKADYFNGMTLEIFVKDPLSFPMDYRATVSNMGIEMNADALMFIPDQETKRYIKEMRGYEPELVTPDNGAKYVDEYTIQLDEMEPLVAAPHSVDNVKVVNELEGTPVDQVYIGSCTNGRLSDFEIAAKIMKGKKVKSRCIAIPASYRMFKEALERGYIQTLVDAGCIVTYGTCGPCLGGHFGIAGPGENIVSTSSRNFKGRMGSNEAKVYLSGPAVAAISALEGKITDPRVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W33</accession>
    <name>Q97W33_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90412">
        <property type="entry name" value="F90412"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989421.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W33"/>
    <dbReference type="SMR" id="Q97W33"/>
    <dbReference type="STRING" id="273057.SSO2409"/>
    <dbReference type="PaxDb" id="273057-SSO2409"/>
    <dbReference type="DNASU" id="1453884"/>
    <dbReference type="EnsemblBacteria" id="AAK42557">
        <property type="protein sequence ID" value="AAK42557"/>
        <property type="gene ID" value="SSO2409"/>
    </dbReference>
    <dbReference type="GeneID" id="1453884"/>
    <dbReference type="KEGG" id="sso:SSO2409"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2492"/>
    <dbReference type="eggNOG" id="arCOG05952">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850668_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W33"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="15249" checksum="F4DCBE5A4B433C19" modified="2001-10-01" version="1">MDKSLVAAEYLLRARRTLKEAQIAFEDGDLYYTVIRTLDTVENLAKVLLSLRDVFKYDLFWNTALFLNDGSMLEKKLRDLEYRLIPITLINESSLKTPTVVIRNKEAEMLVDEISKILEEVEKIYDEFHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W41</accession>
    <name>Q97W41_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC-2 type transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2404</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90411">
        <property type="entry name" value="F90411"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W41"/>
    <dbReference type="STRING" id="273057.SSO2404"/>
    <dbReference type="PaxDb" id="273057-SSO2404"/>
    <dbReference type="EnsemblBacteria" id="AAK42549">
        <property type="protein sequence ID" value="AAK42549"/>
        <property type="gene ID" value="SSO2404"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2404"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2484"/>
    <dbReference type="eggNOG" id="arCOG01462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055390_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W41"/>
    <dbReference type="PhylomeDB" id="Q97W41"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471:SF3">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN NATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="356"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="6">
        <location>
            <begin position="19"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="397" mass="43593" checksum="52824F74CBC9DB9A" modified="2001-10-01" version="1">MMLRTMIKKEILDLKRDRKLLLGTIVLPFVLLPLIGIILYASIAVSPPVIEIINYNQSNLPYIQIISNYITRNGGTVIYNNTNTSIVPDAVIIFPNDFNINASNISRQTFVYEKILISSNQEASNLVNNAIGYLSYTLVYNRIERLINSSNLQHVVNPDDVINPLLVKNFVVTITGRNASQSQANLSEMARIITLVLFPSATPVIFYVTDGIVGEKERKTLESLLASPISINSFIFSKVIIAVILGVLSSLGDILGIVIFSSLMSFTFGLPLSLSTPFILIVILTYLLAVLLTAALSVILLLALGGSMRNMQVINFLILSFGLIASFSALFINVANLQSPLNLVLLIPYEQLSLSLLYFVSGSTFVSLSLLLGVFAVCVIILIVSSRLFNSERLLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W93</accession>
    <name>Q97W93_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2344</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90404">
        <property type="entry name" value="H90404"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989515.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W93"/>
    <dbReference type="STRING" id="273057.SSO2344"/>
    <dbReference type="PaxDb" id="273057-SSO2344"/>
    <dbReference type="EnsemblBacteria" id="AAK42495">
        <property type="protein sequence ID" value="AAK42495"/>
        <property type="gene ID" value="SSO2344"/>
    </dbReference>
    <dbReference type="GeneID" id="1453822"/>
    <dbReference type="KEGG" id="sso:SSO2344"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2428"/>
    <dbReference type="eggNOG" id="arCOG05960">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1076116_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W93"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="261" mass="29863" checksum="A2449A6ED82E55F5" modified="2001-10-01" version="1">MFLLQQSEDSTSIKKLVRFFNINFPQMDLIVYGRGLELPAFTLGRALSSLKNKMINVLNIYDFLFICNPYIEVSNVVAFTGDENELRDLLDGLESLRVNGRIYFCGITNIKPRYNFVSITSFDKSFCEINLSLSILKILSNDNIKREKMILDQLNDISDLDDYVKNYANVFSLDQPILLSPIMLPAKSFLESLGVNVSSYFDTKILDSVQIVYTGVDMHIIKRVIFSLRSKGKKVTDILIDADPLLAPIYLSMIMFYLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WD7</accession>
    <name>Q97WD7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-oxoacyl-(Acyl carrier protein) reductase (FabG-6)</fullName>
            <ecNumber evidence="3">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fabG-6</name>
        <name evidence="3" type="ordered locus">SSO2289</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90399">
        <property type="entry name" value="D90399"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923822.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WD7"/>
    <dbReference type="FunCoup" id="Q97WD7">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2289"/>
    <dbReference type="PaxDb" id="273057-SSO2289"/>
    <dbReference type="EnsemblBacteria" id="AAK42451">
        <property type="protein sequence ID" value="AAK42451"/>
        <property type="gene ID" value="SSO2289"/>
    </dbReference>
    <dbReference type="GeneID" id="1453778"/>
    <dbReference type="KEGG" id="sso:SSO2289"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2383"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_2"/>
    <dbReference type="InParanoid" id="Q97WD7"/>
    <dbReference type="PhylomeDB" id="Q97WD7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005056">
        <property type="entry name" value="PRK06463.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF133">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="259" mass="28787" checksum="FA00904F8C4878FC" modified="2001-10-01" version="1">MVVSYHMRFKDKVILITGGTRGIGRAITEAFLREGGLPIVLYNSAEDEAKKLREKGVFTIKCDVGNREDVKKSKEIVEKEFRRVNVVVNNAGIMLTMPFEEFDEEKYNKMIRINLNGTIYTTYEFLPLLKASENGAIVNIASNAGIGTAAEGTTFYAITKASIIILTKRLAFELGKYGIRVNAVAPGWVETDMTLSGKSKEEVERLRDLFRNKTVLKTTGKPEDIANIVLFLASNEARYITGQVIVADGGRIDNLTHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WK6</accession>
    <name>Q97WK6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90390">
        <property type="entry name" value="E90390"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992074.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WK6"/>
    <dbReference type="STRING" id="273057.SSO2210"/>
    <dbReference type="PaxDb" id="273057-SSO2210"/>
    <dbReference type="EnsemblBacteria" id="AAK42380">
        <property type="protein sequence ID" value="AAK42380"/>
        <property type="gene ID" value="SSO2210"/>
    </dbReference>
    <dbReference type="GeneID" id="1453707"/>
    <dbReference type="KEGG" id="sso:SSO2210"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2306"/>
    <dbReference type="eggNOG" id="arCOG05913">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431570_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WK6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="187" mass="22082" checksum="A5295C6E2E301A74" modified="2001-10-01" version="1">MQTTSVEIYLNIYSFRRELEHFTIEEERDEWLIVKDKANEKYIVKEFADYGILIYPIYDLKDDILSSFSFQLSSVSKLKEVLYTPEKWIDRLDLRINDNSIEVTSLILDYLTGIDIINSLISSFGFEYAQLDDNSLIIKIRISRPLNHTLLDSYIKAIWHMLELYYSVKKAQEDIASKITLNYIKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WN6</accession>
    <name>Q97WN6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lysine exporter LysO family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90386">
        <property type="entry name" value="G90386"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992126.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WN6"/>
    <dbReference type="STRING" id="273057.SSO2175"/>
    <dbReference type="PaxDb" id="273057-SSO2175"/>
    <dbReference type="DNASU" id="1453677"/>
    <dbReference type="EnsemblBacteria" id="AAK42350">
        <property type="protein sequence ID" value="AAK42350"/>
        <property type="gene ID" value="SSO2175"/>
    </dbReference>
    <dbReference type="GeneID" id="1453677"/>
    <dbReference type="KEGG" id="sso:SSO2175"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2271"/>
    <dbReference type="eggNOG" id="arCOG01615">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078428_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WN6"/>
    <dbReference type="PhylomeDB" id="Q97WN6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015661">
        <property type="term" value="F:L-lysine efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005642">
        <property type="entry name" value="LysO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35804">
        <property type="entry name" value="LYSINE EXPORTER LYSO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35804:SF1">
        <property type="entry name" value="LYSINE EXPORTER LYSO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03956">
        <property type="entry name" value="Lys_export"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="33245" checksum="B923418827FB6CFB" modified="2001-10-01" version="1">MNGSSIFFIVLYLIFLIIGKIKYVKEAERIVDIVVILLILTISYWAGEEITSNQVFSLVISSLILSIFSIIITYFSGVAIRSFNNSTKLLVKTNKEKVPNRSNRQTIIKYMLPFISGWIFGLLFHINDNIVINLVDYELYALASVLGYTMGKDVNRKVILNSSKDSLISLFITILGDIILALVIYMLHIANLTISFAIALASGWYSYVGPLVAVSSNPYLGTLAFLINFLREQLTYVLVPFLLRVKFEPRSAVAVGGATAMDTTLPLYVEVLGKEYALSAMITGVILTLIIPIILPLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WS1</accession>
    <name>Q97WS1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90371">
        <property type="entry name" value="H90371"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923751.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WS1"/>
    <dbReference type="STRING" id="273057.SSO2045"/>
    <dbReference type="PaxDb" id="273057-SSO2045"/>
    <dbReference type="EnsemblBacteria" id="AAK42231">
        <property type="protein sequence ID" value="AAK42231"/>
        <property type="gene ID" value="SSO2045"/>
    </dbReference>
    <dbReference type="GeneID" id="1453558"/>
    <dbReference type="KEGG" id="sso:SSO2045"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2126"/>
    <dbReference type="eggNOG" id="arCOG03672">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_334538_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WS1"/>
    <dbReference type="PhylomeDB" id="Q97WS1"/>
    <dbReference type="BRENDA" id="3.4.23.42">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007981">
        <property type="entry name" value="Peptidase_A5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05317">
        <property type="entry name" value="Thermopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="850"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="875" mass="96499" checksum="46EDA4FCA6A39F0B" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WW7</accession>
    <name>Q97WW7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase AAA-type core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99366">
        <property type="entry name" value="A99366"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923713.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WW7"/>
    <dbReference type="STRING" id="273057.SSO1994"/>
    <dbReference type="PaxDb" id="273057-SSO1994"/>
    <dbReference type="EnsemblBacteria" id="AAK42184">
        <property type="protein sequence ID" value="AAK42184"/>
        <property type="gene ID" value="SSO1994"/>
    </dbReference>
    <dbReference type="GeneID" id="1453511"/>
    <dbReference type="KEGG" id="sso:SSO1994"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2070"/>
    <dbReference type="eggNOG" id="arCOG03237">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035814_3_1_2"/>
    <dbReference type="InParanoid" id="Q97WW7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000731">
        <property type="term" value="P:DNA synthesis involved in DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014555">
        <property type="entry name" value="RecF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32182:SF22">
        <property type="entry name" value="ATP-DEPENDENT ENDONUCLEASE, OLD FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32182">
        <property type="entry name" value="DNA REPLICATION AND REPAIR PROTEIN RECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13304">
        <property type="entry name" value="AAA_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029347">
        <property type="entry name" value="RecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase AAA-type core" evidence="1">
        <location>
            <begin position="24"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="328" mass="38261" checksum="2AA09818537241F5" modified="2001-10-01" version="1">MIKRLKIKNFKSYRDSEFEFEKVSIVVGPNGSGKTNLVDAFSFLKQLIRPLSYPPYPFIRWGDYKNVVFMQDENLDISFEIDGEYKGKDYHYEISLNKLQIKKEIINFDSYSMERRGNVIRYENKEMTIPDNLSVFNLFFAQPFIIAYNLNFTLPPELLNFMTNFLNDVGVFRIVPQIAVSPVHFTFPEALDENGRGLVKVISNNLSRINETKQVYDFLVENNISLRPVFTEDGNIRLHFIEKSENNKELILLPASVPDGFIKMLTIIIAVYLLKMSTIIIDEIENSLHLKYIERLVDIMRYSDSQFIATTHSPLIIDFMDPFRNNNS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2024-03-27" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WX4</accession>
    <name>CMR4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR system CMR subunit Cmr4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>CRISPR type III-B/RAMP module RAMP protein Cmr4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cmr4</name>
        <name type="ordered locus">SSO1987</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="Mol. Cell" volume="45" first="303" last="313">
            <title>Structure and mechanism of the CMR complex for CRISPR-mediated antiviral immunity.</title>
            <authorList>
                <person name="Zhang J."/>
                <person name="Rouillon C."/>
                <person name="Kerou M."/>
                <person name="Reeks J."/>
                <person name="Brugger K."/>
                <person name="Graham S."/>
                <person name="Reimann J."/>
                <person name="Cannone G."/>
                <person name="Liu H."/>
                <person name="Albers S.V."/>
                <person name="Naismith J.H."/>
                <person name="Spagnolo L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="22227115"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2011.12.013"/>
        </citation>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION IN CMR COMPLEX</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). The CMR complex degrades RNA complementary to the crRNA (target RNA) within UA dinucleotides, generating 3'-OH and 5'-phosphate ends. Activity is dependent on the 8 nt long 5' tag in the crRNA, an unpaired 3' flag on the target RNA, and is stimulated by ATP. Some cleavage of the guide crRNA can also be observed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the CMR ribonucleoprotein complex, consisting of crRNA plus Cmr1/Cmr2/Cmr3/Cmr4/Cmr5/Cmr6 at 1:1 and possibly 3 Cmr7 dimers. A Cmr2/Cmr3/Cmr7 subcomplex without crRNA can also be isolated. It does not cleave target RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR system Cmr4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90365">
        <property type="entry name" value="B90365"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WX4"/>
    <dbReference type="SMR" id="Q97WX4"/>
    <dbReference type="STRING" id="273057.SSO1987"/>
    <dbReference type="PaxDb" id="273057-SSO1987"/>
    <dbReference type="EnsemblBacteria" id="AAK42177">
        <property type="protein sequence ID" value="AAK42177"/>
        <property type="gene ID" value="SSO1987"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1987"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2063"/>
    <dbReference type="eggNOG" id="arCOG02657">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047795_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WX4"/>
    <dbReference type="PhylomeDB" id="Q97WX4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013410">
        <property type="entry name" value="CRISPR-assoc_RAMP_Cmr4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02580">
        <property type="entry name" value="cas_RAMP_Cmr4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36700">
        <property type="entry name" value="CRISPR SYSTEM CMR SUBUNIT CMR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36700:SF1">
        <property type="entry name" value="CRISPR SYSTEM CMR SUBUNIT CMR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418078" description="CRISPR system CMR subunit Cmr4">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="22227115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="308" mass="34725" checksum="BF65DC97DD6A9222" modified="2001-10-01" version="1">MIIMTYYTFVKPFFIKAVTHLHVSSGTSVEEEVDLPFQRDELNYPTIFASSLKGAIKSFLLKEYSNAREIIYKVLGSDENPDEASLGSFLDAVLFAIPARSIGVSGLDEAWVYVTTYELLRKVKSLLESMNTLSATQFTSLLDAINNILQESKNLIISDNAASVILNEDFYVELSNIQNEYIQSLLGDMLIENSTYNQSNQNSQSQGNHLVKPLIVLKDSIGREVINRSLIRVRRIKIKRSSKTVEEGGLWSEEYVPVNSLFFSSFLARGSKDTAYFADCILRKTRYLILGGKETIGKGIVELRWLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X12</accession>
    <name>Q97X12_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1943</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90360">
        <property type="entry name" value="B90360"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923665.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X12"/>
    <dbReference type="STRING" id="273057.SSO1943"/>
    <dbReference type="PaxDb" id="273057-SSO1943"/>
    <dbReference type="EnsemblBacteria" id="AAK42137">
        <property type="protein sequence ID" value="AAK42137"/>
        <property type="gene ID" value="SSO1943"/>
    </dbReference>
    <dbReference type="GeneID" id="1453464"/>
    <dbReference type="KEGG" id="sso:SSO1943"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2017"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066381_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X12"/>
    <dbReference type="PhylomeDB" id="Q97X12"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="1">
        <location>
            <begin position="236"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="357" mass="41455" checksum="3EC4ACF6E7F5B6A6" modified="2001-10-01" version="1">MVERETEAKSSGSGVTNPYHRTEHCDKIHAYRCDKEVGVIGIDVSKDHLITSRGRVRRYENSKKGYEEILKMKPCTIVLEPTGVYAIKPSQYFKERGVRVLQVSPNVLSREKEFRGKKTDFYDAEKLENMVDKAKEYDYNPLKELVTLYLFLKDIETKYKNRLKRALFLVSDNDKVSKDRLEKLAKGDFTQEELYQLEYTPLVLEEIKILAKNLLETQERLKEVRRMIEGQVPQDHVLLTIPGVGRLAAGVIIGVVGDVRRFPKPESFVAYCGLDPVVERSGKAVVSRGISKRGNKYLRSLFYFLAMRSYSRNPTLLKFYETHKDRLKGKKLYVALARKLARVVWSVWYNNRPYEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X48</accession>
    <name>Q97X48_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4898 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1908</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90355">
        <property type="entry name" value="E90355"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988998.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X48"/>
    <dbReference type="PaxDb" id="273057-SSO1908"/>
    <dbReference type="EnsemblBacteria" id="AAK42100">
        <property type="protein sequence ID" value="AAK42100"/>
        <property type="gene ID" value="SSO1908"/>
    </dbReference>
    <dbReference type="GeneID" id="1453427"/>
    <dbReference type="KEGG" id="sso:SSO1908"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1969"/>
    <dbReference type="eggNOG" id="arCOG08429">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2299469_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X48"/>
    <dbReference type="PhylomeDB" id="Q97X48"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032603">
        <property type="entry name" value="DUF4898"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16239">
        <property type="entry name" value="DUF4898"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="11475" checksum="AD110FEA2D40D757" modified="2001-10-01" version="1">MTAFPIDKLSEYIEDNLIEMIGKSGSVKFVKYVSPGLIVDAQKFFKTFIPFAKYYVVIVPADINNDKVKDELISMAKSSFNFTILYSYKLKDRLLIVGYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-01-24" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X98</accession>
    <name>Q97X98_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1359</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1856</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90348">
        <property type="entry name" value="E90348"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X98"/>
    <dbReference type="PaxDb" id="273057-SSO1856"/>
    <dbReference type="EnsemblBacteria" id="AAK42044">
        <property type="protein sequence ID" value="AAK42044"/>
        <property type="gene ID" value="SSO1856"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1856"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1905"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067987_1_0_2"/>
    <dbReference type="InParanoid" id="Q97X98"/>
    <dbReference type="PhylomeDB" id="Q97X98"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="89"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="257" mass="29596" checksum="4D315F3E1430C749" modified="2001-10-01" version="1">MKHENTTKPAKFNRDFARSALKIIYSILTKILFPEELLSALLKASGSYLSRLGKDGRRALRKLNAVQVEDVRDALRKMGRMTLRGVRDRRVAVDFHAIPQYHADKSFLSRIKPTKGTSWGLVQAAIFLLGRTRSFLDVIPVTVKNVAEGFKAVMEVIVKELEEDKLRLVMVFADREFAVNEVIRYLLELGLDFVISAKAQMYKKYKGMLQDVDVSFGGVRYIWISLRETWEWSLSHYPEEGRRQDYCFPREEGDGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XB1</accession>
    <name>Q97XB1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1834</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90346">
        <property type="entry name" value="G90346"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992713.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XB1"/>
    <dbReference type="STRING" id="273057.SSO1834"/>
    <dbReference type="PaxDb" id="273057-SSO1834"/>
    <dbReference type="EnsemblBacteria" id="AAK42030">
        <property type="protein sequence ID" value="AAK42030"/>
        <property type="gene ID" value="SSO1834"/>
    </dbReference>
    <dbReference type="GeneID" id="1453357"/>
    <dbReference type="KEGG" id="sso:SSO1834"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1894"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123170_2_1_2"/>
    <dbReference type="InParanoid" id="Q97XB1"/>
    <dbReference type="PhylomeDB" id="Q97XB1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="9"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="130" mass="15185" checksum="E553746153679721" modified="2001-10-01" version="1">MVSRYYDWLRQAERNLKSAELNFENGIYEETCYEAQQVAEKSVKSLLSYFHKEMRGHSITFLLQFSSISIPDWILKCAQELDKNYIPSRYPDVYDQGAPLDYYSKDDATSCLECARKILAWVKEIVGTSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XH4</accession>
    <name>Q97XH4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1766</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90338">
        <property type="entry name" value="A90338"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923574.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XH4"/>
    <dbReference type="PaxDb" id="273057-SSO1766"/>
    <dbReference type="EnsemblBacteria" id="AAK41960">
        <property type="protein sequence ID" value="AAK41960"/>
        <property type="gene ID" value="SSO1766"/>
    </dbReference>
    <dbReference type="GeneID" id="1454739"/>
    <dbReference type="KEGG" id="sso:SSO1766"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1814"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154343_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XH4"/>
    <dbReference type="PhylomeDB" id="Q97XH4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="146" mass="16636" checksum="01BCAC9518C0C77D" modified="2001-10-01" version="1">MGFNDVFLLMGYTTSKLKSFTAFRKYKGWGRKHGKSYFGFKVCNLVERRTNFVRDFKVGLANLSDLAFSFDNVKLMADRAWISRKDVLVKGVGSARLPVEGSGVEIREGKASSTTLRGVVMEVFFLNFYRDLEILSTRIRTKVLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XI4</accession>
    <name>Q97XI4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TIGR00266 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90336">
        <property type="entry name" value="F90336"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990004.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XI4"/>
    <dbReference type="FunCoup" id="Q97XI4">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1755"/>
    <dbReference type="PaxDb" id="273057-SSO1755"/>
    <dbReference type="EnsemblBacteria" id="AAK41949">
        <property type="protein sequence ID" value="AAK41949"/>
        <property type="gene ID" value="SSO1755"/>
    </dbReference>
    <dbReference type="GeneID" id="1454728"/>
    <dbReference type="KEGG" id="sso:SSO1755"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1797"/>
    <dbReference type="eggNOG" id="arCOG01907">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040551_0_1_2"/>
    <dbReference type="InParanoid" id="Q97XI4"/>
    <dbReference type="PhylomeDB" id="Q97XI4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="entry name" value="Mitochondrial biogenesis AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00266">
        <property type="entry name" value="TIGR00266 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43657:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43657">
        <property type="entry name" value="TRYPTOPHAN RNA-BINDING ATTENUATOR PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="TRAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="25160" checksum="EE219F27CA14F17F" modified="2001-10-01" version="1">MPQYEILGKDLQYLKVMLGEGEAIYVDAGHMIAKQSSVTMKTTMRGGFFGALKREITGGSFFVTELYGPGEVYLSGVFPGPIVQIQLQGNGILAESHSFLCAENGVNYDATLNKLSVGWLGGEGIFLAKFRGTGNVFLHAYGGLIERYLDPGERLQVEAAHLMAFEEGMRYGIQRVGGIKSILFSGEGLFFVDIEGPGKVWLHTLTVEQLAASLRPYLPQGQQGGPSFNIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XI9</accession>
    <name>Q97XI9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Partial transposase in ISC1234</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1747</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90335">
        <property type="entry name" value="H90335"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XI9"/>
    <dbReference type="PaxDb" id="273057-SSO1747"/>
    <dbReference type="EnsemblBacteria" id="AAK41943">
        <property type="protein sequence ID" value="AAK41943"/>
        <property type="gene ID" value="SSO1747"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1747"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179761_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XI9"/>
    <dbReference type="PhylomeDB" id="Q97XI9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="113" mass="13035" checksum="0B03ACD4EBF36BED" modified="2001-10-01" version="1">MAMIFVSLFVRSITVKPANFSDKRFKSPLKGIKIADKGFSPSPTQLIAREKPFTTLRAHVEFFGTYLNAFWRPYGTTTWRNDVFLHVLGVIYNIKMFLAIQRRTPPGRRAVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XL7</accession>
    <name>Q97XL7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Third ORF in transposon ISC1491</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90331">
        <property type="entry name" value="D90331"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XL7"/>
    <dbReference type="STRING" id="273057.SSO1699"/>
    <dbReference type="PaxDb" id="273057-SSO1699"/>
    <dbReference type="EnsemblBacteria" id="AAK41907">
        <property type="protein sequence ID" value="AAK41907"/>
        <property type="gene ID" value="SSO1699"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1699"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1750"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153635_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XL7"/>
    <dbReference type="PhylomeDB" id="Q97XL7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF17">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="139" mass="15403" checksum="7D3FDE32E5E9E46E" modified="2001-10-01" version="1">MISAATIYAEFGDVSRFSSSKAARAYAGFAPRTKQSGESESHSGMIRGNKYLRRAFFLVARVARKSERFKEYYDRLVSKWKSATKATCALGGKLACICYHVIKDGVYKGDVKKSFRAPRGKDVNVQDVDVGDVLDSLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XN7</accession>
    <name>Q97XN7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1676</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90328">
        <property type="entry name" value="G90328"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989129.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XN7"/>
    <dbReference type="STRING" id="273057.SSO1676"/>
    <dbReference type="PaxDb" id="273057-SSO1676"/>
    <dbReference type="EnsemblBacteria" id="AAK41886">
        <property type="protein sequence ID" value="AAK41886"/>
        <property type="gene ID" value="SSO1676"/>
    </dbReference>
    <dbReference type="GeneID" id="1454665"/>
    <dbReference type="KEGG" id="sso:SSO1676"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1723"/>
    <dbReference type="eggNOG" id="arCOG04025">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_485422_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XN7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="561" mass="64855" checksum="493FB4530F4D5C3D" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XS3</accession>
    <name>Q97XS3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1636</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90324">
        <property type="entry name" value="B90324"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923536.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XS3"/>
    <dbReference type="PaxDb" id="273057-SSO1636"/>
    <dbReference type="EnsemblBacteria" id="AAK41849">
        <property type="protein sequence ID" value="AAK41849"/>
        <property type="gene ID" value="SSO1636"/>
    </dbReference>
    <dbReference type="GeneID" id="1454628"/>
    <dbReference type="KEGG" id="sso:SSO1636"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1681"/>
    <dbReference type="eggNOG" id="arCOG08453">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_528569_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XS3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="484"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="515" mass="56710" checksum="4675B72242947FAA" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XW9</accession>
    <name>Q97XW9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5808 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90318">
        <property type="entry name" value="A90318"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XW9"/>
    <dbReference type="STRING" id="273057.SSO1590"/>
    <dbReference type="PaxDb" id="273057-SSO1590"/>
    <dbReference type="EnsemblBacteria" id="AAK41800">
        <property type="protein sequence ID" value="AAK41800"/>
        <property type="gene ID" value="SSO1590"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1590"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1625"/>
    <dbReference type="eggNOG" id="arCOG06014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_805659_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XW9"/>
    <dbReference type="PhylomeDB" id="Q97XW9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043831">
        <property type="entry name" value="DUF5808"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014574">
        <property type="entry name" value="UCP032908"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37810">
        <property type="entry name" value="IMMUNITY PROTEIN SDPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37810:SF5">
        <property type="entry name" value="IMMUNITY PROTEIN SDPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19124">
        <property type="entry name" value="DUF5808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032908">
        <property type="entry name" value="UCP032908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5808" evidence="2">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="347" mass="39583" checksum="E1315912FB7BF736" modified="2001-10-01" version="1">MKNMTLLDIIIYVEFTFLFINLFLLPNLQPPDILLGARVTTEFKKNKGKDIVNKYRLSLIGLTLLSLLLYYFVSFISVFIYVILLLLNVVYWRRIVIRRRGSLPVASSRYAVIDTKKKESRKWIITFIVAWGILIFTLVFGSLVYSSAPKLLPTHVGPTGTTFLVKTPLNFYKVEIINTVVLGLLTVLGVFITRTTNVINPAYPDRSYYAFLKFKEEVGIIAGIVGVFMTSLYTTISLFIWTIINLSELEVFIIFLISMLFTVIIVYVLRIGIEGARYLRDVIANDNLLNDDKYWKGGIIYVNPKDPRIWVPKRNGLGYTLNFGHGISILIILAVVFAVITTIILVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y13</accession>
    <name>Q97Y13_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1537</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90312">
        <property type="entry name" value="G90312"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992920.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y13"/>
    <dbReference type="FunCoup" id="Q97Y13">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1537"/>
    <dbReference type="PaxDb" id="273057-SSO1537"/>
    <dbReference type="EnsemblBacteria" id="AAK41758">
        <property type="protein sequence ID" value="AAK41758"/>
        <property type="gene ID" value="SSO1537"/>
    </dbReference>
    <dbReference type="GeneID" id="1454537"/>
    <dbReference type="KEGG" id="sso:SSO1537"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1575"/>
    <dbReference type="eggNOG" id="arCOG00511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_3_2_2"/>
    <dbReference type="InParanoid" id="Q97Y13"/>
    <dbReference type="PhylomeDB" id="Q97Y13"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07729">
        <property type="entry name" value="AHL_lactonase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051013">
        <property type="entry name" value="MBL_superfamily_lactonases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978:SF2">
        <property type="entry name" value="102 KBASES UNSTABLE REGION: FROM 1 TO 119443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978">
        <property type="entry name" value="QUORUM-QUENCHING LACTONASE YTNP-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="6">
        <location>
            <begin position="43"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="269" mass="31048" checksum="3776FEB34C95F147" modified="2001-10-01" version="1">MSVSKIYLLDHGKISADLAWFLPNPMTIEDMKNPKPRNWVDVSVMSAVIEHKDGVILFDTGIPDNVSGKWPQIALSVFPVTKYSEENKMENQLKKIGYKPEDIHFIILSHLHLDHTGSLELFKNSKPDVIVHEKELKYALLNLWLNKPVPYLLRDLEILREMNIVPMSTEYLEILPGVELLLIGGHTPGSILLKVRTQNDNNYIFTGDFIHVPEELSFESKGWLLGNAEEYYTRIRLLKSMQKLPRTNIIISHDPKLWEKYPKAPKELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y15</accession>
    <name>Q97Y15_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Multidrug resistance transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1535</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90312">
        <property type="entry name" value="E90312"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992166.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y15"/>
    <dbReference type="FunCoup" id="Q97Y15">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1535"/>
    <dbReference type="EnsemblBacteria" id="AAK41756">
        <property type="protein sequence ID" value="AAK41756"/>
        <property type="gene ID" value="SSO1535"/>
    </dbReference>
    <dbReference type="GeneID" id="1454535"/>
    <dbReference type="KEGG" id="sso:SSO1535"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1573"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_34_0_2"/>
    <dbReference type="InParanoid" id="Q97Y15"/>
    <dbReference type="PhylomeDB" id="Q97Y15"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17504">
        <property type="entry name" value="MFS_MMR_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF35">
        <property type="entry name" value="BLL0718 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01036">
        <property type="entry name" value="TCRTETB"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="8"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="474" mass="52278" checksum="8CC0D4FF3EEEA41D" modified="2001-10-01" version="1">MERAFLRYVPILAFITIMTMYVEMVILPSLPEIENQFSITSSEASWVLSSETLAGMTLAPFLGKLADSYGRKKVLISILVVYILSVFLTAISPNYSILLISRSIQGIGLSINPIAYTILRERIPDRELPIAQGIIASTFAIGAAVALPIGSYISQYFSWEFAYETAIPLLILSTIIIYVILPSSESKGSDRKIDDVGVILLSLSFLILGIGLTEAPTWGWTSLQFFTSLLLSILLFYLFALHISKSRNPIIDPYDFKNPNVAVPLISSFITGFGLFLTFQSLVFIFELPSPIGYGMTILQTGETMAPIALIILIAGPFFGRLVTKIGYMKVILTSSSVSSFLLLFLAYIISLRISVLELMGFLSLILFFISGMNVTRVTLLIASSKRERMSTLTGTNTSMRLMGNTLGPIVVGSLEDTFKTPIFEGYLNNIPIFAFVPSRLAFEYAFLISMATTVLVTILATRIRERPSTTTYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y73</accession>
    <name>Q97Y73_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3782 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99305">
        <property type="entry name" value="A99305"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993098.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y73"/>
    <dbReference type="STRING" id="273057.SSO1469"/>
    <dbReference type="PaxDb" id="273057-SSO1469"/>
    <dbReference type="EnsemblBacteria" id="AAK41696">
        <property type="protein sequence ID" value="AAK41696"/>
        <property type="gene ID" value="SSO1469"/>
    </dbReference>
    <dbReference type="GeneID" id="1454475"/>
    <dbReference type="KEGG" id="sso:SSO1469"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1505"/>
    <dbReference type="eggNOG" id="arCOG01424">
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    <dbReference type="HOGENOM" id="CLU_965117_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y73"/>
    <dbReference type="PhylomeDB" id="Q97Y73"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38753">
        <property type="entry name" value="SLR1441 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38753:SF1">
        <property type="entry name" value="SLR1441 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="36"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="32641" checksum="8EC450182980DC91" modified="2001-10-01" version="1">MSEKIIDELLNNPQLLSVLAEKIYGRLKDEIVIKKLEENSEAIRALQETVNKHTEAIQSLQEAVKEQGKAIQALQQVTQSLQETVNKHTEAIQSLQEAVKEQGKAIQALQQVTQSLQETVNKHTEAIQSLQEAVKKQGEAIEALQKAVLRLGREVKKLSIELGSFTNRAGKGMEKAMLKLYRKSLELHSVDPKKVAHGKIVDDEGVIEKGKVFEVDFYETNEYVYVFEIKNFADKGTYDQIIVRKKLFSAKYKNKKIKIFVVANFVDEKVKKKLEEEGVEVIASHVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YA3</accession>
    <name>Q97YA3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cas10/Cmr2 second palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1429</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2023" name="Curr. Res. Struct. Biol." volume="5" first="100098" last="100098">
            <title>Structure of the &lt;i&gt;Saccharolobus solfataricus&lt;/i&gt; type III-D CRISPR effector.</title>
            <authorList>
                <person name="Cannone G."/>
                <person name="Kompaniiets D."/>
                <person name="Graham S."/>
                <person name="White M.F."/>
                <person name="Spagnolo L."/>
            </authorList>
            <dbReference type="PubMed" id="36843655"/>
            <dbReference type="DOI" id="10.1016/j.crstbi.2023.100098"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (3.50 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90300">
        <property type="entry name" value="H90300"/>
    </dbReference>
    <dbReference type="PDB" id="8BMW">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="K=1-294"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YA3"/>
    <dbReference type="SMR" id="Q97YA3"/>
    <dbReference type="STRING" id="273057.SSO1429"/>
    <dbReference type="PaxDb" id="273057-SSO1429"/>
    <dbReference type="DNASU" id="1454442"/>
    <dbReference type="EnsemblBacteria" id="AAK41663">
        <property type="protein sequence ID" value="AAK41663"/>
        <property type="gene ID" value="SSO1429"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1429"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1456"/>
    <dbReference type="eggNOG" id="arCOG02666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_945339_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YA3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054767">
        <property type="entry name" value="Cas10-Cmr2_palm2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22335">
        <property type="entry name" value="Cas10-Cmr2_palm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="6" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas10/Cmr2 second palm" evidence="3">
        <location>
            <begin position="15"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="8BMW"/>
        </source>
    </evidence>
    <sequence length="294" mass="33479" checksum="13454196C9415E42" modified="2001-10-01" version="1">MEIIAGYRTTSDSRYIALIKADGNNAGKIFGNTVTFSEYVDKSFRLDFGVKKMFYDTLLDIMRASSDESIKKDLVSRILLGVLYLGGDDIMLLSPSAIAVPFAVKMFKRSLEYTGFTFKVGIISVKPDHPVQFAYGAVNALMEESKIHTGEKSSIGVLVFSSTLASEGVVKSDLKNYRKEKESFLVVSNDVDDVERLLNLMELDDFGKLMELYWNPEEGRKVIRDKIRSLERFVNYADTHDFYNTLAYLIRSKAKSEENSLIKRIIDLTIKGRDDFVFPLYDYYFILKSIRVGI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2019-01-16" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YD2</accession>
    <name>RN393_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR system ring nuclease SSO1393</fullName>
            <ecNumber evidence="3">4.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO1393</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2018" name="Nature" volume="562" first="277" last="280">
            <title>Ring nucleases deactivate type III CRISPR ribonucleases by degrading cyclic oligoadenylate.</title>
            <authorList>
                <person name="Athukoralage J.S."/>
                <person name="Rouillon C."/>
                <person name="Graham S."/>
                <person name="Grueschow S."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="30232454"/>
            <dbReference type="DOI" id="10.1038/s41586-018-0557-5"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>MUTAGENESIS OF SER-11 AND LYS-168</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2011-03" db="PDB data bank">
            <title>Crystal structure of the CRISPR-associated protein SSO1393 from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Petit P."/>
                <person name="Xu X."/>
                <person name="Beloglazova N."/>
                <person name="Brown G."/>
                <person name="Savchenko A."/>
                <person name="Yakunin A.F."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). A nuclease that degrades cyclic oligoadenylates (cOA), second messengers that induce an antiviral state important for defense against invading nucleic acids. Destruction of cOA deactivates the Csx1 ribonuclease, preventing uncontrolled degradation of cellular RNA. Slowly degrades cA4 (a tetraadenylate ring) into first a linear tetraadenylate product and secondly into a linear diadenylate product with 5'-OH and 2',3'-cyclic phosphate termini. Is 10-fold less active than SSO2081, suggesting it plays a minor role in cA4 degradation. There may be 2 active sites per homodimer (PubMed:30232454).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cyclic tetraadenylate = 2 5'-hydroxy-diadenylate 2',3'-cylic phosphate</text>
            <dbReference type="Rhea" id="RHEA:58012"/>
            <dbReference type="ChEBI" id="CHEBI:142457"/>
            <dbReference type="ChEBI" id="CHEBI:142458"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <text evidence="1">Does not require a metal cofactor.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <text evidence="1">kcat is 0.024 min(-1).</text>
        </kinetics>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cOA ring nuclease family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.-" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90296">
        <property type="entry name" value="F90296"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990074.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3QYF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A/B=1-321"/>
    </dbReference>
    <dbReference type="PDBsum" id="3QYF"/>
    <dbReference type="AlphaFoldDB" id="Q97YD2"/>
    <dbReference type="SMR" id="Q97YD2"/>
    <dbReference type="STRING" id="273057.SSO1393"/>
    <dbReference type="PaxDb" id="273057-SSO1393"/>
    <dbReference type="EnsemblBacteria" id="AAK41629">
        <property type="protein sequence ID" value="AAK41629"/>
        <property type="gene ID" value="SSO1393"/>
    </dbReference>
    <dbReference type="GeneID" id="1454408"/>
    <dbReference type="KEGG" id="sso:SSO1393"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1409"/>
    <dbReference type="eggNOG" id="arCOG01935">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082641_1_0_2"/>
    <dbReference type="InParanoid" id="Q97YD2"/>
    <dbReference type="PhylomeDB" id="Q97YD2"/>
    <dbReference type="EvolutionaryTrace" id="Q97YD2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09742">
        <property type="entry name" value="Csm6_III-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.196.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10770">
        <property type="entry name" value="Hypothetical protein VC1899 like domain (Restriction endonuclease-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1690">
        <property type="entry name" value="Uncharacterised CRISPR-associated protein family, UPF0236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016620">
        <property type="entry name" value="SSO1393"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013442">
        <property type="entry name" value="SSO1393-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054041">
        <property type="entry name" value="WHD_SSO1393"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040951">
        <property type="entry name" value="CRSPR_nucase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02619">
        <property type="entry name" value="putative CRISPR-associated protein, APE2256 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09651">
        <property type="entry name" value="Cas_APE2256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22165">
        <property type="entry name" value="WHD_SSO1393"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF014470">
        <property type="entry name" value="UCP014470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000446012" description="CRISPR system ring nuclease SSO1393">
        <location>
            <begin position="1"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="4">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="32-fold decrease in kcat for degradation of cA4." evidence="1">
        <original>S</original>
        <variation>A</variation>
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No degradation of cA4." evidence="1">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="2"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="11"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="16"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="20"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="34"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="44"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="67"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="87"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="119"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="146"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="158"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="164"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="185"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="turn" evidence="9">
        <location>
            <begin position="191"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="195"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="204"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="208"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="222"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="226"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="236"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="244"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="turn" evidence="9">
        <location>
            <begin position="249"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="252"/>
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        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="257"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="turn" evidence="9">
        <location>
            <begin position="264"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="30232454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="30232454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="30232454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5">
        <source ref="3"/>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="3QYF"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="3QYF"/>
        </source>
    </evidence>
    <sequence length="321" mass="37448" checksum="4D50669446A9BA64" modified="2001-10-01" version="1">MEVHVCSVGTSLLKNSLDDDNVRKEIERLGLKDWDRLKFDDDRQNRIKENFDSLRKMLLKFIRSKGRRASAELDSLFSTFEKLKHNKSEIYVFLYSTNTSNSQLAGEVIRDYLIEEGIRSELVTVKTISSEENFYEGIVDLFDKVIYRILKFKEQDNEVYINATPGLKPESIFLTLAGLLAGADLIYYKYQEFNDVVILPSPPITIRPKYLDWLIRFAISGYTLSEKRAEELGIPVRLLEAKMLVERKGEDAYRLKDWVRKLLGIYLPIGAQNKYYRVIVEGEGERTFDNEVEAYNYMESKRKEGKNVRVEVPDRVYFLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YE9</accession>
    <name>Q97YE9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1376</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="C90294"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="GeneID" id="1454389"/>
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    <dbReference type="HOGENOM" id="CLU_745186_0_0_2"/>
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        <property type="component" value="Chromosome"/>
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    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025904">
        <property type="entry name" value="Tubulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13809">
        <property type="entry name" value="Tubulin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="116"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="371" mass="41489" checksum="8631A2C7B4B2976B" modified="2001-10-01" version="1">MLEVVYIVGAFGSPLLTPPTLAVAGFLIAVLMGLEYGVLWVLTGESLNRELILVISNIVYSIAWSITALPFTPIYYTLQPYFSQPLLIYLYLALFIGSILLGVIPAIMYIRKYLQFNKIQKEFKQLELDYKSINQEYSEINTRLNKAKEEINNVMDKINKAKELHETALRDPDLIVTALDKKPEIPEVIRPNVNYVVAGLGGTGTALLEALIDYLVSKNVIKEDGTNPFLFVAFDTNQASIARLRKKYEYTPVGKLLYTFNNFEALTTANIISHNPWLAGLNVDVIQGSGMKRAIGYASYNTVKEAMMEDILRRLSDLIAKTRTNRIFLIVFTALGGGTGSGSFIHFTKDFISRLGRITGDSEPYVRVSGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YF1</accession>
    <name>Q97YF1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1374</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
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            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99294">
        <property type="entry name" value="A99294"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990012.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YF1"/>
    <dbReference type="STRING" id="273057.SSO1374"/>
    <dbReference type="PaxDb" id="273057-SSO1374"/>
    <dbReference type="EnsemblBacteria" id="AAK41608">
        <property type="protein sequence ID" value="AAK41608"/>
        <property type="gene ID" value="SSO1374"/>
    </dbReference>
    <dbReference type="GeneID" id="1454387"/>
    <dbReference type="KEGG" id="sso:SSO1374"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1379"/>
    <dbReference type="HOGENOM" id="CLU_982183_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YF1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="283" mass="30972" checksum="2A592C563B955F31" modified="2001-10-01" version="1">MMMEKIVMVAILILSIISLPLTIIYPHASSSLHLGPQRICFNITSNNYVSAVYKPIIQVLNLNARPNSTVNITLSLTNSSMPMMLKVPKFNLLGYTLGGKLVTINVNPLGSLCYGIPDLSISTPVFSAGVYMVFSGYITGVLSFSGNISKPLLIRLTDAGNLSIPIKIPLHASGKGIITLSNITYHVAISLVVKYSSLLNSGNYTILPLKPIGNFKGVPSYINGIYVIESNSYMTLLILYLYIVIAVGMIIAIIVKFRRKIIHIITFLKHFVKLTNPCLDKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YK6</accession>
    <name>Q97YK6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Second ORF in transposon ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO3256</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90287">
        <property type="entry name" value="B90287"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YK6"/>
    <dbReference type="SMR" id="Q97YK6"/>
    <dbReference type="STRING" id="273057.SSO3256"/>
    <dbReference type="PaxDb" id="273057-SSO3256"/>
    <dbReference type="EnsemblBacteria" id="AAK41553">
        <property type="protein sequence ID" value="AAK41553"/>
        <property type="gene ID" value="SSO3256"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3256"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1318"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387635_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YK6"/>
    <dbReference type="PhylomeDB" id="Q97YK6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="5">
        <location>
            <begin position="12"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="196" mass="23550" checksum="60219B94BFC20522" modified="2001-10-01" version="1">MITSSRPFHLLGKVVAIDVGIEKLVTTSDGQYFPNLKPFERALTKVRELHRSLSRKRFLSHNWFKAKVKLARVYEHVKNLRKDIYMKLGKYFAEYYDIVVMEDISVKKLVGKSLKVRRRLHDVGFYEFRTILEYQLIKYGKKFQWCFTNFTQNDTISMEEKNIRAELEKSAYFNTRSKKFSYLLKKLTENEFIYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YS5</accession>
    <name>Q97YS5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIP18 family-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90277">
        <property type="entry name" value="H90277"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923265.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YS5"/>
    <dbReference type="STRING" id="273057.SSO1234"/>
    <dbReference type="PaxDb" id="273057-SSO1234"/>
    <dbReference type="EnsemblBacteria" id="AAK41479">
        <property type="protein sequence ID" value="AAK41479"/>
        <property type="gene ID" value="SSO1234"/>
    </dbReference>
    <dbReference type="GeneID" id="1454258"/>
    <dbReference type="KEGG" id="sso:SSO1234"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1233"/>
    <dbReference type="eggNOG" id="arCOG01845">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1709223_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YS5"/>
    <dbReference type="PhylomeDB" id="Q97YS5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052339">
        <property type="entry name" value="Fe-S_Maturation_MIP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831:SF1">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="153" mass="18272" checksum="CF4E268A39981822" modified="2001-10-01" version="1">MKEKVVEILRQIYDPEIPINIYDLGLVREIRIEGKRIFVRLIFTANKGCTLADLVAVQVKYKLMKVFPDYNVEVKSDFNEEWNIGYATETGRLMLEEIYGKDAVEVLVNKTKIEELVSTNKVKLENFDPREYMRKAVEERYKKFREWYDKHKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YX0</accession>
    <name>Q97YX0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">First ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1181</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90271">
        <property type="entry name" value="G90271"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923232.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YX0"/>
    <dbReference type="PaxDb" id="273057-SSO1181"/>
    <dbReference type="DNASU" id="1454209"/>
    <dbReference type="EnsemblBacteria" id="AAK41430">
        <property type="protein sequence ID" value="AAK41430"/>
        <property type="gene ID" value="SSO1181"/>
    </dbReference>
    <dbReference type="GeneID" id="1454209"/>
    <dbReference type="KEGG" id="sso:SSO1181"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1179"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="InParanoid" id="Q97YX0"/>
    <dbReference type="PhylomeDB" id="Q97YX0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04762">
        <property type="entry name" value="HTH_MerR-trunc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1660.10:FF:000013">
        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2170:FF:000002">
        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1390:FF:000002">
        <property type="entry name" value="ORF1 in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="5">
        <location>
            <begin position="55"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="195" mass="22977" checksum="E27387BEC44FC100" modified="2001-10-01" version="1">MLRPKEACQRLGISYATLREYVKKGYIKPVILQSGKWRFREEDVERLMGIIRKRKVILYARVSSNTQKDDLVNQVKYLEEQVKEYDLVITDIGSKLNMKRKGFLKLLRMILNNEVSRVVVTYSDRLVRFGFEILEEVCKAHNCEIVVLNQEDKEEELVEDLVSILVSLSGKLYGMRSHEYEKVKKCAEELKNWKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YY3</accession>
    <name>Q97YY3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sugar ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">SSO1169</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. MalFG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90270">
        <property type="entry name" value="B90270"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YY3"/>
    <dbReference type="FunCoup" id="Q97YY3">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1169"/>
    <dbReference type="PaxDb" id="273057-SSO1169"/>
    <dbReference type="EnsemblBacteria" id="AAK41417">
        <property type="protein sequence ID" value="AAK41417"/>
        <property type="gene ID" value="SSO1169"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1169"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1161"/>
    <dbReference type="eggNOG" id="arCOG00160">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_2_2"/>
    <dbReference type="InParanoid" id="Q97YY3"/>
    <dbReference type="PhylomeDB" id="Q97YY3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050901">
        <property type="entry name" value="BP-dep_ABC_trans_perm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32243">
        <property type="entry name" value="MALTOSE TRANSPORT SYSTEM PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32243:SF50">
        <property type="entry name" value="MALTOSE_MALTODEXTRIN TRANSPORT SYSTEM PERMEASE PROTEIN MALG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="150"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="196"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="85"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK41417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="296" mass="32837" checksum="3E39790A6429B68F" modified="2001-10-01" version="1">MIHMMQIKKYRLFKILRLAISYLVLIIMAAFSIFPLYYVFMTSFSNAPNLISLAVSDLLPKHIYFTAYKYLFTTNLYGGSFPLWIRNSLILAGSTAIISVLLALITGYALSRLNVPAKKILATFIYIVTFFPYTASAVPLYLLFAKFHLLNYIGLILAYTPGTSIFAAFIAKLSIDSIPPSYEEIAMIDGLSRFGAFLRIVMRLALPVVALTALLGFSGTYLDFALAYSFLLPNVKEWTATIGLYYMAGLLNQASAPAYNIFAAGAVIMGIPLMALFIVSQRMMTRAYSNLAGVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z00</accession>
    <name>Q97Z00_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SRPBCC family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99268">
        <property type="entry name" value="A99268"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923213.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z00"/>
    <dbReference type="PaxDb" id="273057-SSO1149"/>
    <dbReference type="EnsemblBacteria" id="AAK41400">
        <property type="protein sequence ID" value="AAK41400"/>
        <property type="gene ID" value="SSO1149"/>
    </dbReference>
    <dbReference type="GeneID" id="1454179"/>
    <dbReference type="KEGG" id="sso:SSO1149"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1141"/>
    <dbReference type="eggNOG" id="arCOG05400">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131895_1_0_2"/>
    <dbReference type="InParanoid" id="Q97Z00"/>
    <dbReference type="PhylomeDB" id="Q97Z00"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="17185" checksum="24AAD746B85E3778" modified="2001-10-01" version="1">MFLSLSEIIIIMIEFVIEKSFNMPEDIVWRIARDVNSIPNYWKGVRELNVKEVDKNVYEGRIRFAFPSSGKVRIIINDDERTLTFEYLDGPIRGYNKVKISKGKISSEWKVTMTGLLKLTEGWNRDHFMEGTEHALEMILNATLSLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z46</accession>
    <name>Q97Z46_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine/threonine phosphatase type 1</fullName>
            <ecNumber evidence="2">3.1.3.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.16" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90261">
        <property type="entry name" value="G90261"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923195.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z46"/>
    <dbReference type="FunCoup" id="Q97Z46">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1090"/>
    <dbReference type="PaxDb" id="273057-SSO1090"/>
    <dbReference type="EnsemblBacteria" id="AAK41350">
        <property type="protein sequence ID" value="AAK41350"/>
        <property type="gene ID" value="SSO1090"/>
    </dbReference>
    <dbReference type="GeneID" id="1454129"/>
    <dbReference type="KEGG" id="sso:SSO1090"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1088"/>
    <dbReference type="eggNOG" id="arCOG01143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_0_10_2"/>
    <dbReference type="InParanoid" id="Q97Z46"/>
    <dbReference type="PhylomeDB" id="Q97Z46"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00144">
        <property type="entry name" value="MPP_PPP_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000232">
        <property type="entry name" value="Serine/threonine phosphatase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF496">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="1">
        <location>
            <begin position="97"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="269" mass="30554" checksum="47033B6578B33EF0" modified="2001-10-01" version="1">MMNLDEVKSVVSNAIEIYKSKYDSPFVGNFEVDGNVVFVGDTHGAIDVTSKVFSDFAENADLVVFLGDYVDRGEDQLGNLLLILRKMIEKPDKYIVLRGNHESPITNEYYGFKEEVKEKLGEENYEMFVDLFSYMPYAVVVNGYFCIHGGLPMGLEKVEEIKKLPRPDVNPDNPIAFQLLWNDPREGIDDLDFLPSIRGEGIYFFGKRVVDNFLENNSLKGIIRGHEAADGFRTDMGGKVITVFSSRYHHMRAGILVLKKDGEFDYAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z91</accession>
    <name>Q97Z91_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA 2',3'-cyclic phosphodiesterase</fullName>
            <shortName evidence="2">RNA 2',3'-CPDase</shortName>
            <ecNumber evidence="2">3.1.4.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO1040</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-end 2'-phospho-ribonucleotide-RNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11828"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17353"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173113"/>
            <dbReference type="EC" id="3.1.4.58"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 2H phosphoesterase superfamily. ThpR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.58" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90255">
        <property type="entry name" value="G90255"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989192.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z91"/>
    <dbReference type="STRING" id="273057.SSO1040"/>
    <dbReference type="PaxDb" id="273057-SSO1040"/>
    <dbReference type="EnsemblBacteria" id="AAK41302">
        <property type="protein sequence ID" value="AAK41302"/>
        <property type="gene ID" value="SSO1040"/>
    </dbReference>
    <dbReference type="GeneID" id="1454081"/>
    <dbReference type="KEGG" id="sso:SSO1040"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1034"/>
    <dbReference type="eggNOG" id="arCOG01736">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081251_3_4_2"/>
    <dbReference type="InParanoid" id="Q97Z91"/>
    <dbReference type="PhylomeDB" id="Q97Z91"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008664">
        <property type="term" value="F:RNA 2',3'-cyclic 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1140.10:FF:000009">
        <property type="entry name" value="RNA 2',3'-cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01940">
        <property type="entry name" value="RNA_CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014051">
        <property type="entry name" value="Phosphoesterase_HXTX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004175">
        <property type="entry name" value="RNA_CPDase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02258">
        <property type="entry name" value="2_5_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561">
        <property type="entry name" value="RNA 2',3'-CYCLIC PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561:SF1">
        <property type="entry name" value="RNA 2',3'-CYCLIC PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02834">
        <property type="entry name" value="LigT_PEase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoesterase HXTX" evidence="3">
        <location>
            <begin position="9"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoesterase HXTX" evidence="3">
        <location>
            <begin position="92"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 1" evidence="2">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 2" evidence="2">
        <location>
            <begin position="124"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="194" mass="22099" checksum="2D7CCE6468B7A25B" modified="2001-10-01" version="1">MRLFIGIEVPQSPKLLEIFEIVKNAGADIKLVEPYNIHITLLFLGEVREDRVEEVKDSMSGLKFNKFKVTLKGLGAFPNLTRPRVVWVGIVEGQQQLRQIRSYILNELLKRKIRPEDDKDFSPHLTIGRVKSYNSISNLINTINENMNVDIGTFEVNNVVLFKSTLTPKGPIYDKLFEVSSIDRGGSTEDNKTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZB9</accession>
    <name>Q97ZB9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1004</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90252">
        <property type="entry name" value="C90252"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989234.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZB9"/>
    <dbReference type="STRING" id="273057.SSO1004"/>
    <dbReference type="PaxDb" id="273057-SSO1004"/>
    <dbReference type="EnsemblBacteria" id="AAK41274">
        <property type="protein sequence ID" value="AAK41274"/>
        <property type="gene ID" value="SSO1004"/>
    </dbReference>
    <dbReference type="GeneID" id="1455241"/>
    <dbReference type="KEGG" id="sso:SSO1004"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1000"/>
    <dbReference type="eggNOG" id="arCOG00340">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_606425_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZB9"/>
    <dbReference type="PhylomeDB" id="Q97ZB9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF118">
        <property type="entry name" value="ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE (PRECURSOR)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="5">
        <location>
            <begin position="41"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="411" mass="46527" checksum="D89697A09345B308" modified="2001-10-01" version="1">MFMMKLDELEKELGSKLIMDNNVIDHYSKSPYLVSPVLSKMGKRVLGVVIAEDRYDIEFVVKFCDIHRIPLLARGAGTSTIGQVLPIHPSIVLDIQKLNKTMEYDEKYLKISPGVKVLDALNYLRKRGKELQVYPSSFYISTLGGYIAGGDVGIGSYQYGYHFHNGGIRRVKVVGSTGTFELTGENTLAVAQAAGTTGIIVEADIATVDYEDWKDQLVRFDELGKLVKFLKDVENERDKIRRITIEDQEALSLVAKNRAIPGKWNVILASTKSFGEEVEMKFLDELAFAAIYVTMSRLTNFSEYFYEVRLLSLDSFLKVVSQVKNALGSNVLIHGDVMTLRGETVIYTVFISDRQNFNIIDSIMTKEGIPFEIHSLVVNDRVDEEYRLELMKKYKRIVDPHNILNPGKLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZD0</accession>
    <name>Q97ZD0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glucan 1,4 alpha glucosidase (Glucoamylase)</fullName>
            <ecNumber evidence="2">3.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.2.1.3" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90250">
        <property type="entry name" value="G90250"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989251.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZD0"/>
    <dbReference type="SMR" id="Q97ZD0"/>
    <dbReference type="STRING" id="273057.SSO0990"/>
    <dbReference type="CAZy" id="GH15">
        <property type="family name" value="Glycoside Hydrolase Family 15"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO0990"/>
    <dbReference type="EnsemblBacteria" id="AAK41262">
        <property type="protein sequence ID" value="AAK41262"/>
        <property type="gene ID" value="SSO0990"/>
    </dbReference>
    <dbReference type="GeneID" id="1455229"/>
    <dbReference type="KEGG" id="sso:SSO0990"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.987"/>
    <dbReference type="eggNOG" id="arCOG03285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028187_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZD0"/>
    <dbReference type="PhylomeDB" id="Q97ZD0"/>
    <dbReference type="BRENDA" id="3.2.1.3">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004339">
        <property type="term" value="F:glucan 1,4-alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616:SF13">
        <property type="entry name" value="GLUCAN 1,4-ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH15-like" evidence="1">
        <location>
            <begin position="280"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="622" mass="72053" checksum="C7B411FD2B1ABE8F" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZJ3</accession>
    <name>ENO_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enolase</fullName>
            <ecNumber evidence="1">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eno</name>
        <name type="ordered locus">SSO0913</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible conversion of 2-phosphoglycerate (2-PG) into phosphoenolpyruvate (PEP). It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds a second Mg(2+) ion via substrate during catalysis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <text evidence="1">Fractions of enolase are present in both the cytoplasm and on the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90242">
        <property type="entry name" value="D90242"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992342.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZJ3"/>
    <dbReference type="SMR" id="Q97ZJ3"/>
    <dbReference type="FunCoup" id="Q97ZJ3">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0913"/>
    <dbReference type="PaxDb" id="273057-SSO0913"/>
    <dbReference type="EnsemblBacteria" id="AAK41195">
        <property type="protein sequence ID" value="AAK41195"/>
        <property type="gene ID" value="SSO0913"/>
    </dbReference>
    <dbReference type="GeneID" id="1455162"/>
    <dbReference type="GeneID" id="44129843"/>
    <dbReference type="KEGG" id="sso:SSO0913"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.915"/>
    <dbReference type="eggNOG" id="arCOG01169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031223_2_1_2"/>
    <dbReference type="InParanoid" id="Q97ZJ3"/>
    <dbReference type="PhylomeDB" id="Q97ZJ3"/>
    <dbReference type="BRENDA" id="4.2.1.11">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <feature type="chain" id="PRO_0000134035" description="Enolase">
        <location>
            <begin position="1"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <sequence length="419" mass="46745" checksum="BBD4685C8AA9B075" modified="2001-10-01" version="1">MINRFSIEKVKGLEIVDSRGNPTIRVFIRTSDGVESFGDAPAGASKGTREAVEVRDENGLTVKRAVDIVNYIIDPALHGIDVREQGIIDKLLKDIDSTENKSKLGGNTIIATSIAALKTASKALGLEVFKYISGPRLPKIPIPLLNIINGGLHAGNKLKIQEFIIVPIKFNTFKEALFAAIDVYRTLKGLITERYGKIYTAVGDEGGFSPPLEDTREALDLIYTSINNAGYEGKIYMGMDAAGSDFYDSKKEKYIIDGRELDPNQLLEFYLDLVKQYPIVYLEDPFEENSFDMFSQLQNKLSSTIITGDDLYTTNIKYLKIGIEKRSTKGVIVKPNQVGTISETFEFTNLARRNSMKLITSHRSGETEDNFIADFAVGIESDFIKVGAPARGERTSKYNKLLEIENKFGLEYEGKYFYL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2026-01-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZK4</accession>
    <name>RS34_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein aS34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps34a</name>
        <name evidence="4" type="ordered locus">SSO6759</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7 8 9" key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>DISULFIDE BONDS</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8 9 10 11 12 13 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7 8 9 10 11 12 13 14">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S small ribosomal subunit (PubMed:39753558). Interacts with uS3 and uS14 (PubMed:39753558).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ribosomal protein aS34 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90241">
        <property type="entry name" value="A90241"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992325.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="a=1-72"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="a=1-72"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="a=1-72"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="a=1-72"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="a=1-72"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="a=1-72"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="a=1-72"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="a=1-72"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q97ZK4"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q97ZK4"/>
    <dbReference type="STRING" id="273057.SSO6759"/>
    <dbReference type="PaxDb" id="273057-SSO6759"/>
    <dbReference type="EnsemblBacteria" id="AAK41184">
        <property type="protein sequence ID" value="AAK41184"/>
        <property type="gene ID" value="SSO6759"/>
    </dbReference>
    <dbReference type="GeneID" id="1455151"/>
    <dbReference type="KEGG" id="sso:SSO6759"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.904"/>
    <dbReference type="eggNOG" id="arCOG04103">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2629676_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZK4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00874">
        <property type="entry name" value="Ribosomal_aS34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000463551" description="Small ribosomal subunit protein aS34">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 8 9">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8 9">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8 9">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8 9">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8 10">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8 9">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8 9">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8 9">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="10">
        <source>
            <dbReference type="PDB" id="9FRL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="11">
        <source>
            <dbReference type="PDB" id="9FS6"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="12">
        <source>
            <dbReference type="PDB" id="9FS8"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="13">
        <source>
            <dbReference type="PDB" id="9FSF"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="14">
        <source>
            <dbReference type="PDB" id="9FY0"/>
        </source>
    </evidence>
    <sequence length="72" mass="8197" checksum="13D0F3447A968AEE" modified="2001-10-01" version="1">MSIKNRGSNAYGHLGWLTVYCRICNRKLIIGTDILYKCPKCEKKYSAYFCEADKRGLKGKCPYCGTELVPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZM4</accession>
    <name>Q97ZM4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Replication initiator protein WhiP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0867</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90237">
        <property type="entry name" value="E90237"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992287.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZM4"/>
    <dbReference type="STRING" id="273057.SSO0867"/>
    <dbReference type="PaxDb" id="273057-SSO0867"/>
    <dbReference type="EnsemblBacteria" id="AAK41156">
        <property type="protein sequence ID" value="AAK41156"/>
        <property type="gene ID" value="SSO0867"/>
    </dbReference>
    <dbReference type="GeneID" id="1455123"/>
    <dbReference type="KEGG" id="sso:SSO0867"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.877"/>
    <dbReference type="eggNOG" id="arCOG04268">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1109465_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZM4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="28835" checksum="F36FEE75EBC28AF9" modified="2001-10-01" version="1">MSEDYRKDDIEAIAQQISEEEKPGKESPRSKLVEAILLLLYARPLRTAEIAANLGYETKYISSYLSYWKKKGLVYQEGGRWHLSGKGENIARDIIESQNNSKFKEYLLLAKQVLESEKVRQTKNNKVEKKDDKKAQEVLWFIEGKTSKENKKQQKSNPTDCIKEILNKLDDDEKEILSHLLNKYREWGTTYIYLDQLQEEMKADTSWLFRVLKNLQIKRLLYIYQDPRMGVRIGLSKSIKETISSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZP1</accession>
    <name>Q97ZP1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO6661</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90235">
        <property type="entry name" value="B90235"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923132.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZP1"/>
    <dbReference type="STRING" id="273057.SSO6661"/>
    <dbReference type="PaxDb" id="273057-SSO6661"/>
    <dbReference type="EnsemblBacteria" id="AAK41137">
        <property type="protein sequence ID" value="AAK41137"/>
        <property type="gene ID" value="SSO6661"/>
    </dbReference>
    <dbReference type="GeneID" id="1455104"/>
    <dbReference type="KEGG" id="sso:SSO6661"/>
    <dbReference type="HOGENOM" id="CLU_2646100_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZP1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="76" mass="8046" checksum="7090B3F3F156F45A" modified="2001-10-01" version="1">MLINYDITLLVAFSSANCVPLVSFKPNVANADIIPHNAKNVITIQEFAKYCEGSPSRPTSSIGIKPPSTAAANCIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZQ9</accession>
    <name>Q97ZQ9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">7-carboxy-7-deazaguanine synthase</fullName>
            <shortName evidence="8">CDG synthase</shortName>
            <ecNumber evidence="8">4.3.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Archaeosine biosynthesis protein QueE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">queE</name>
        <name evidence="10" type="ordered locus">SSO0624</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>6-carboxy-5,6,7,8-tetrahydropterin + H(+) = 7-carboxy-7-carbaguanine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:27974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="EC" id="4.3.99.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </cofactor>
        <text evidence="8">Binds 1 S-adenosyl-L-methionine per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="8">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.3.99.3" evidence="8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90209">
        <property type="entry name" value="G90209"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZQ9"/>
    <dbReference type="FunCoup" id="Q97ZQ9">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0624"/>
    <dbReference type="PaxDb" id="273057-SSO0624"/>
    <dbReference type="EnsemblBacteria" id="AAK40934">
        <property type="protein sequence ID" value="AAK40934"/>
        <property type="gene ID" value="SSO0624"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0624"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.630"/>
    <dbReference type="eggNOG" id="arCOG02173">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066739_2_3_2"/>
    <dbReference type="InParanoid" id="Q97ZQ9"/>
    <dbReference type="PhylomeDB" id="Q97ZQ9"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:tRNA queuosine(34) biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00917">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024924">
        <property type="entry name" value="7-CO-7-deazaguanine_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42836">
        <property type="entry name" value="7-CARBOXY-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42836:SF1">
        <property type="entry name" value="7-CARBOXY-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000370">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="9">
        <location>
            <begin position="21"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="15"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="40"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="119"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK40934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="211" mass="24066" checksum="928541313698DA55" modified="2001-10-01" version="1">MVQMKYRVIEIFTSIQGEGEVIGIPSNFIRLATCNLRCVWCDTKYSWEIGTEMSIDEIIAKLDKKIKTTTITGGEPLLQNILPLAKELKSIGHKIVVETNGTIKPSEELRKIIDIFSVSPKLSNSGHKLKYDFADDWATYYKFVIVYPNKDIDEVVKFVESQSINPRKVILQPDGNRSDYINAINEIVQIVLDRGLQFRVLPQLHRIIGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZW8</accession>
    <name>Q97ZW8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF72 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0453</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90190">
        <property type="entry name" value="E90190"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZW8"/>
    <dbReference type="STRING" id="273057.SSO0453"/>
    <dbReference type="PaxDb" id="273057-SSO0453"/>
    <dbReference type="EnsemblBacteria" id="AAK40780">
        <property type="protein sequence ID" value="AAK40780"/>
        <property type="gene ID" value="SSO0453"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0453"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.449"/>
    <dbReference type="eggNOG" id="arCOG04291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1192595_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZW8"/>
    <dbReference type="PhylomeDB" id="Q97ZW8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.410">
        <property type="entry name" value="Protein of unknown function UPF0759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002763">
        <property type="entry name" value="DUF72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036520">
        <property type="entry name" value="UPF0759_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348:SF4">
        <property type="entry name" value="DUF72 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YECE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01904">
        <property type="entry name" value="DUF72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117396">
        <property type="entry name" value="TM1631-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="31587" checksum="6750721FBC7386A4" modified="2001-10-01" version="1">MFSSSLTSYVYTKPIILIFSQNLCKMIKIGTCGFTRKHFNYFDVLEVQETFYNFLSEERLNKLKELSIRNKVELTIKANQIITHEYNRITYKRFKKIIGDVKNYGYFRPTKEVMQALEITLNEAKFLNSRIIIFQTPPSFAPNNENIKNLKDFFSILDKSFIYGWEPRGEWNQNHEVLLKIFSQIDVIHVVDPFKNKSVDDKQIRYFRLHGLGSSEVNYRYKYTKADLEKLKEYVLSERKELIYVLFNNVYSFDDALSFKRMIEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2021-09-29" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q980A3</accession>
    <name>RPO7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo7</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">DNA-directed RNA polymerase, subunit E'</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo7</name>
        <name evidence="1" type="synonym">rpoE</name>
        <name type="ordered locus">SSO0415</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2008" name="Nature" volume="451" first="851" last="854">
            <title>The X-ray crystal structure of RNA polymerase from Archaea.</title>
            <authorList>
                <person name="Hirata A."/>
                <person name="Klein B.J."/>
                <person name="Murakami K.S."/>
            </authorList>
            <dbReference type="PubMed" id="18235446"/>
            <dbReference type="DOI" id="10.1038/nature06530"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.40 ANGSTROMS) OF THE RNA POLYMERASE COMPLEX</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 13-subunit RNA polymerase complex. Forms a stalk with Rpo4 that extends from the main structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1 2">Forms 2 domains with an elongated structure; Rpo4 packs into the hinge region between the 2 domains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90185">
        <property type="entry name" value="G90185"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988767.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2PMZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="E/T=1-180"/>
    </dbReference>
    <dbReference type="PDB" id="3HKZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="E/Q=1-180"/>
    </dbReference>
    <dbReference type="PDBsum" id="2PMZ"/>
    <dbReference type="PDBsum" id="3HKZ"/>
    <dbReference type="AlphaFoldDB" id="Q980A3"/>
    <dbReference type="SMR" id="Q980A3"/>
    <dbReference type="DIP" id="DIP-60644N"/>
    <dbReference type="FunCoup" id="Q980A3">
        <property type="interactions" value="260"/>
    </dbReference>
    <dbReference type="IntAct" id="Q980A3">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0415"/>
    <dbReference type="PaxDb" id="273057-SSO0415"/>
    <dbReference type="EnsemblBacteria" id="AAK40742">
        <property type="protein sequence ID" value="AAK40742"/>
        <property type="gene ID" value="SSO0415"/>
    </dbReference>
    <dbReference type="GeneID" id="1455552"/>
    <dbReference type="KEGG" id="sso:SSO0415"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.409"/>
    <dbReference type="eggNOG" id="arCOG00675">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117966_0_0_2"/>
    <dbReference type="InParanoid" id="Q980A3"/>
    <dbReference type="PhylomeDB" id="Q980A3"/>
    <dbReference type="BRENDA" id="2.7.7.6">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q980A3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04331">
        <property type="entry name" value="RNAP_E_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04460">
        <property type="entry name" value="S1_RpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00865">
        <property type="entry name" value="RNApol_arch_Rpo7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004519">
        <property type="entry name" value="RNAP_E/RPC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046399">
        <property type="entry name" value="RNApol_Rpo7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006333">
        <property type="entry name" value="PRK08563.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00448">
        <property type="entry name" value="rpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000453813" description="DNA-directed RNA polymerase subunit Rpo7">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="82"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="2"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="15"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="18"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="40"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="44"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="55"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="89"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="94"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="102"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="111"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="122"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="130"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="144"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="160"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="166"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="172"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="176"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18235446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="18235446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="3HKZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="3HKZ"/>
        </source>
    </evidence>
    <sequence length="180" mass="20329" checksum="2351BE9ADA4847C7" modified="2001-10-01" version="1">MYKLIKARSIVRIPPNEFGKPLNEIALNELRQQYQEKILKDLGLVLAILNVKTSEEGILVFGDGATYHEVEFDMITYVPVVQEVVEGEVLQVDNYGIFVNLGPMDGLVHISQITDDTLKYDNVRGIIFGEKSKKVIQKGDKVRARVISVASTVTGRLPRIALTMRQPYLGKLEWITQTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q980J0</accession>
    <name>Q980J0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phospho-2-dehydro-3-deoxyheptonate aldolase /chorismate mutase (AroG)</fullName>
            <ecNumber evidence="3">2.5.1.54</ecNumber>
            <ecNumber evidence="3">5.4.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">aroG</name>
        <name evidence="3" type="ordered locus">SSO0304</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.54" evidence="3"/>
    <dbReference type="EC" id="5.4.99.5" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90173">
        <property type="entry name" value="B90173"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990596.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980J0"/>
    <dbReference type="FunCoup" id="Q980J0">
        <property type="interactions" value="113"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0304"/>
    <dbReference type="PaxDb" id="273057-SSO0304"/>
    <dbReference type="EnsemblBacteria" id="AAK40641">
        <property type="protein sequence ID" value="AAK40641"/>
        <property type="gene ID" value="SSO0304"/>
    </dbReference>
    <dbReference type="GeneID" id="1455451"/>
    <dbReference type="KEGG" id="sso:SSO0304"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.297"/>
    <dbReference type="eggNOG" id="arCOG01049">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062599_0_0_2"/>
    <dbReference type="InParanoid" id="Q980J0"/>
    <dbReference type="PhylomeDB" id="Q980J0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052899">
        <property type="entry name" value="Class-I_DAHP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006268">
        <property type="entry name" value="DAHP_syn_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01361">
        <property type="entry name" value="DAHP_synth_Bsub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006421">
        <property type="entry name" value="PRK08673.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009239">
        <property type="entry name" value="PRK12595.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43018">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43018:SF2">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="2">
        <location>
            <begin position="79"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="331" mass="36265" checksum="4C191B10E26BF135" modified="2001-10-01" version="1">MILYVLKDRADYSILIEKLNENSASFKILNLYGKNLILAWPDQNVKGIIDNSIEMAVEVKKSYVLAGNDWKKQPTVVNVKDVEIGSKKVIVAAGPCAVENEEQVLTTAKAVKRAGASLLRGGAYKPRTSPYSFQGLGEEGVKILRRVGDEVGLPIVTEIMDTRDSNIFSQYVDMIQIGARNAQNFSLLKEVGKLGKPVLLKRGMGNTVEEWLQAAEYILLEGNGNTVLCERGIRTFEKSTRFTLDIGGMVAAKLMTHLPICADPSHPAGKRELVHSLALAAVAAGADMLLIEVHPHPEKALSDSEQQLTPESFEVLMNRIRTLARALGRDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q980K6</accession>
    <name>Q980K6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">Fructose-1,6-bisphosphate aldolase/phosphatase</fullName>
            <shortName evidence="15">FBP A/P</shortName>
            <shortName evidence="15">FBP aldolase/phosphatase</shortName>
            <ecNumber evidence="6 15">3.1.3.11</ecNumber>
            <ecNumber evidence="15">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">fbp</name>
        <name evidence="16" type="ordered locus">SSO0286</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 15">Homooctamer; dimer of tetramers.</text>
    </comment>
    <comment type="domain">
        <text evidence="15">Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the FBP aldolase/phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="6 15"/>
    <dbReference type="EC" id="4.1.2.13" evidence="15"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90171">
        <property type="entry name" value="B90171"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990571.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980K6"/>
    <dbReference type="SMR" id="Q980K6"/>
    <dbReference type="FunCoup" id="Q980K6">
        <property type="interactions" value="199"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0286"/>
    <dbReference type="PaxDb" id="273057-SSO0286"/>
    <dbReference type="EnsemblBacteria" id="AAK40625">
        <property type="protein sequence ID" value="AAK40625"/>
        <property type="gene ID" value="SSO0286"/>
    </dbReference>
    <dbReference type="GeneID" id="1455435"/>
    <dbReference type="GeneID" id="44129258"/>
    <dbReference type="KEGG" id="sso:SSO0286"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.281"/>
    <dbReference type="eggNOG" id="arCOG04180">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041630_0_0_2"/>
    <dbReference type="InParanoid" id="Q980K6"/>
    <dbReference type="PhylomeDB" id="Q980K6"/>
    <dbReference type="BRENDA" id="4.1.2.13">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q980K6"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02067">
        <property type="entry name" value="FBP_aldolase_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002803">
        <property type="entry name" value="FBPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036076">
        <property type="entry name" value="FBPase_V_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041126">
        <property type="entry name" value="FBP_aldo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38341">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE/PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38341:SF1">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01950">
        <property type="entry name" value="FBPase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015647">
        <property type="entry name" value="FBPtase_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111249">
        <property type="entry name" value="Sulfolobus fructose-1,6-bisphosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 15" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="11 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 15" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton acceptor; for FBP phosphatase activity" evidence="15">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor; for FBP aldolase activity" evidence="15">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DHAP; for FBP aldolase activity" evidence="15">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="103"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="241"/>
            <end position="242"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AAK40625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="382" mass="42708" checksum="4F26A35A37B7EE94" modified="2001-10-01" version="1">MKTTLSVIKADIGSLAGHHVVHPDTIAAANKILAEAKRNNIISDYYITYVGDDLQLIMTHNRGELDTRVHETAWNAFKEAAKIAKDLGLYAAGQDLLSDSFSGNLRGMGPGIAEIEFEERPAEPVVIFMADKTEPGAYNLPLYKIFADPFNTAGLVIDPTMHEGYKFEVLDVYEGESIMLNTPEESYDLLALIGTPSRYIVRRVYRKADNLQASVVSVERLNLIAGKYVGKDDPVMVVRAQHGLPALGEILEAFTVPYLVPGWMRGSHYGPLMPVSQKDARATRFDGPPRLISLGFNIKNGKLVGPTDLFDDPAFDEVRRTAVIIADYIRKHGPFMPHRLEPQEMEYTTLPLVIEKLKNRFKKETDMYKTKPSVYSHESSHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q980L0</accession>
    <name>Q980L0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0283</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90170">
        <property type="entry name" value="F90170"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980L0"/>
    <dbReference type="STRING" id="273057.SSO0283"/>
    <dbReference type="PaxDb" id="273057-SSO0283"/>
    <dbReference type="EnsemblBacteria" id="AAK40621">
        <property type="protein sequence ID" value="AAK40621"/>
        <property type="gene ID" value="SSO0283"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0283"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.277"/>
    <dbReference type="eggNOG" id="arCOG00286">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_504016_0_0_2"/>
    <dbReference type="InParanoid" id="Q980L0"/>
    <dbReference type="PhylomeDB" id="Q980L0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051162">
        <property type="entry name" value="T4SS_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121:SF11">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJGR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="231"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="542" mass="61905" checksum="D4FF77B173A520A0" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q980M8</accession>
    <name>Q980M8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integrase, probable(Amino-end)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0262</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90168">
        <property type="entry name" value="C90168"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980M8"/>
    <dbReference type="SMR" id="Q980M8"/>
    <dbReference type="PaxDb" id="273057-SSO0262"/>
    <dbReference type="EnsemblBacteria" id="AAK40602">
        <property type="protein sequence ID" value="AAK40602"/>
        <property type="gene ID" value="SSO0262"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0262"/>
    <dbReference type="eggNOG" id="arCOG07277">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167324_0_0_2"/>
    <dbReference type="InParanoid" id="Q980M8"/>
    <dbReference type="PhylomeDB" id="Q980M8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012922">
        <property type="entry name" value="ORF_D-335"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07935">
        <property type="entry name" value="SSV1_ORF_D-335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ORF D-335-like" evidence="1">
        <location>
            <begin position="9"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="99" mass="11199" checksum="46B742B80CCD77FD" modified="2001-10-01" version="1">MIREFPKTTNGDVIIRERKGKYYVYKLETINGEVKETYVGPLADVVETYEKLKDSSGGVRVSPTTWARWDLNPGPPPCQGDVLTRLDDGPLILGYCFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q980P4</accession>
    <name>Q980P4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD-dependent protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90166">
        <property type="entry name" value="A90166"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990501.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980P4"/>
    <dbReference type="SMR" id="Q980P4"/>
    <dbReference type="FunCoup" id="Q980P4">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0244"/>
    <dbReference type="PaxDb" id="273057-SSO0244"/>
    <dbReference type="EnsemblBacteria" id="AAK40584">
        <property type="protein sequence ID" value="AAK40584"/>
        <property type="gene ID" value="SSO0244"/>
    </dbReference>
    <dbReference type="GeneID" id="1455394"/>
    <dbReference type="KEGG" id="sso:SSO0244"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.239"/>
    <dbReference type="eggNOG" id="arCOG02231">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046973_1_0_2"/>
    <dbReference type="InParanoid" id="Q980P4"/>
    <dbReference type="PhylomeDB" id="Q980P4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028348">
        <property type="entry name" value="FAD-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049516">
        <property type="entry name" value="FAD-depend_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43106">
        <property type="entry name" value="DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43106:SF1">
        <property type="entry name" value="DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21688">
        <property type="entry name" value="FAD-depend_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038984">
        <property type="entry name" value="FAD_binding_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent protein C-terminal" evidence="1">
        <location>
            <begin position="263"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="469" mass="51942" checksum="9E9E2054575602B5" modified="2001-10-01" version="1">MSSYDVVIIGGGPAGLFAAYELAGLAKDNVSNYKILLVDKGARASKRTCPLLSPKEKCTFCDPCHIMYGFGGAGTFSSGIINLRPDIGGELHEITRSWDKAQELINYVDDIFVKFGAPKDRVFEPNMEKVREIQRRAAKVGAEFVPIRQRHMGTDKTPLIIENIVNYVEKRGIKIGELTEVIDIEKKGNKFFLKTSRGEIESRIVLVAPGRAGAKWFYEQSKKLGVDTIPGPLDIGVRVETESFVFDELTEAVWDPKVILYSKRYDDKVRTFCVNPRGYIMKEVYDDGTIGVNGETYVDKKSNNTNFAFLTTIKLSDPLEDTIEYGKSIARLMTRLGGGKPILQRLIDFQKGRRSTWERINRSTVKPTLRDVTPGDISMGLPYRVVDNLIDGLERLDSIAPGIFSSNTLLYAPEIKYYSVRAVVDNNMETVVDNLFAAGDGTGLSRGINVAAATGILAARGIAIKLGLD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q980T4</accession>
    <name>Y193_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein SSO0193</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0193</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90160">
        <property type="entry name" value="D90160"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990431.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980T4"/>
    <dbReference type="SMR" id="Q980T4"/>
    <dbReference type="FunCoup" id="Q980T4">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0193"/>
    <dbReference type="PaxDb" id="273057-SSO0193"/>
    <dbReference type="EnsemblBacteria" id="AAK40539">
        <property type="protein sequence ID" value="AAK40539"/>
        <property type="gene ID" value="SSO0193"/>
    </dbReference>
    <dbReference type="GeneID" id="1455349"/>
    <dbReference type="KEGG" id="sso:SSO0193"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.192"/>
    <dbReference type="eggNOG" id="arCOG01336">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095686_1_1_2"/>
    <dbReference type="InParanoid" id="Q980T4"/>
    <dbReference type="PhylomeDB" id="Q980T4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="entry name" value="Hypothetical protein ph0010, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.150">
        <property type="entry name" value="Hypothetical protein ph0010, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00645">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027623">
        <property type="entry name" value="AmmeMemoSam_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023472">
        <property type="entry name" value="Uncharacterised_MJ0810"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04335">
        <property type="entry name" value="AmmeMemoSam_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00296">
        <property type="entry name" value="TIGR00296 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016:SF0">
        <property type="entry name" value="AMME SYNDROME CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="AMMECR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="AMMECR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142388" description="Protein SSO0193">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="AMMECR1" evidence="1">
        <location>
            <begin position="15"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00645"/>
        </source>
    </evidence>
    <sequence length="227" mass="25617" checksum="17410EE11DD88390" modified="2001-10-01" version="1">MIQEELVQIQELNEEIGRLLIEIARKSIKEEFKLDKLDLSKYDNPILDKKGLAFVTLEKIAYNTSSLRGCIGYVEAVAPLKQIAASAAKAAAFSDPRFNPLQEDELANIIIEVTVLTKPEEIKVKDRWDLPKIIKVGEDGLIVEKGILHSGLLLPQVPMEYCWDEETFLAETCIKASLEPDCWLDSSVRIKRFHGIIFKETKPNGSDIIVIKPSDIKCKLNELINNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q981B5</accession>
    <name>Q981B5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90142">
        <property type="entry name" value="F90142"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981B5"/>
    <dbReference type="PaxDb" id="273057-SSO0032"/>
    <dbReference type="EnsemblBacteria" id="AAK40397">
        <property type="protein sequence ID" value="AAK40397"/>
        <property type="gene ID" value="SSO0032"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0032"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.33"/>
    <dbReference type="eggNOG" id="arCOG05904">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1029035_0_0_2"/>
    <dbReference type="InParanoid" id="Q981B5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="268" mass="31153" checksum="24CB45AF2FABDEB5" modified="2001-10-01" version="1">MMMDSLKLLSKYDNLTKILELTKEYASKLSLVFAIHAYFENEIISNVVKSLESKVKNIYEDYKFDRTLFVKNASKTLGIKEDDFAYYPYYAIPISKETKVKFIDNSTIPPKALIMKGVVRFTFMAYRSFQELEDHVASREEEDIVIEFENGKIKSHNRKRNIFTDANVVSKIISSNKEVLLNLTLPSSYYLIPSLVSMNVLPYENEVLVIREGESLDFRIINGKVSGDRVVMGDTLHPRFKLELYYDYKFKRILKEEIAEGLAYKIPL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q981B9</accession>
    <name>PURT_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Formate-dependent phosphoribosylglycinamide formyltransferase</fullName>
            <ecNumber evidence="1">6.3.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5'-phosphoribosylglycinamide transformylase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Formate-dependent GAR transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">GAR transformylase 2</fullName>
            <shortName evidence="1">GART 2</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-folate glycinamide ribonucleotide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylglycinamide formyltransferase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purT</name>
        <name type="ordered locus">SSO0026</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(1)-(5-phospho-beta-D-ribosyl)glycinamide + formate + ATP = N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24829"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:24830"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PurK/PurT family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40393" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.3.1.21" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40393.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90142">
        <property type="entry name" value="B90142"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989613.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981B9"/>
    <dbReference type="SMR" id="Q981B9"/>
    <dbReference type="STRING" id="273057.SSO0026"/>
    <dbReference type="PaxDb" id="273057-SSO0026"/>
    <dbReference type="EnsemblBacteria" id="AAK40393">
        <property type="protein sequence ID" value="AAK40393"/>
        <property type="gene ID" value="SSO0026"/>
    </dbReference>
    <dbReference type="GeneID" id="1455284"/>
    <dbReference type="GeneID" id="44128989"/>
    <dbReference type="KEGG" id="sso:SSO0026"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.29"/>
    <dbReference type="eggNOG" id="arCOG01598">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011534_1_3_2"/>
    <dbReference type="InParanoid" id="Q981B9"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00127"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043815">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase 2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000022">
        <property type="entry name" value="Formate-dependent phosphoribosylglycinamide formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01643">
        <property type="entry name" value="PurT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005862">
        <property type="entry name" value="PurT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054350">
        <property type="entry name" value="PurT/PurK_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048740">
        <property type="entry name" value="PurT_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006766">
        <property type="entry name" value="PRK09288.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43055">
        <property type="entry name" value="FORMATE-DEPENDENT PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43055:SF1">
        <property type="entry name" value="FORMATE-DEPENDENT PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21244">
        <property type="entry name" value="PurT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22660">
        <property type="entry name" value="RS_preATP-grasp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000319288" description="Formate-dependent phosphoribosylglycinamide formyltransferase">
        <location>
            <begin position="1"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="1">
        <location>
            <begin position="118"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="21"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="194"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="368"/>
            <end position="369"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="397" mass="44043" checksum="F1936F697D6D6783" modified="2008-02-26" version="2">MEIGTPLFEGSKKILLLGSGELGKEMAIEAQRMGLEVVALDRYDLAPAMHVAHRKYVVDMMNPNAIKAVVKRERPDAIIAEIEAINTDALIELESNGFRVVPNANAVKACMNRIELRRFAAEKLKLPTTKYAFAENEEEVKRACKDIGFPCLIKPEMSSSGHGHVLVNKIENVEEAYRESISHARGKSRRVIVEEFVKIDTELTVLTYRYHSNSDSIITKTIEPIEHKRPSYYYVESWHPSNVSQGVKETARGIAQKVAEELGGLGIYGVEIIVSGNRILFSEVAPRPHDTGLVTLASSDINEFQIHVRSAIGLPTPEVKLVSPAASHVILAQTENVWGPKFLNIEKAMEIPGVQVRLFGKPVTYEKRRMGVVLATGNSVEEALEKVRKASSIILVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-01-24" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UWW3</accession>
    <name>AATI_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">A-type ATP synthase subunit I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">atpI</name>
        <name type="ordered locus">SSO0559</name>
        <name type="ORF">C21_040</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90202">
        <property type="entry name" value="E90202"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991070.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UWW3"/>
    <dbReference type="SMR" id="Q9UWW3"/>
    <dbReference type="FunCoup" id="Q9UWW3">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0559"/>
    <dbReference type="PaxDb" id="273057-SSO0559"/>
    <dbReference type="EnsemblBacteria" id="AAK40876">
        <property type="protein sequence ID" value="AAK40876"/>
        <property type="gene ID" value="SSO0559"/>
    </dbReference>
    <dbReference type="GeneID" id="1454843"/>
    <dbReference type="KEGG" id="sso:SSO0559"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.569"/>
    <dbReference type="eggNOG" id="arCOG04138">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025558_0_0_2"/>
    <dbReference type="InParanoid" id="Q9UWW3"/>
    <dbReference type="PhylomeDB" id="Q9UWW3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004435">
        <property type="entry name" value="PRK05771.3-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000119235" description="A-type ATP synthase subunit I">
        <location>
            <begin position="1"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="363"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="435"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="555"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="583"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="612"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="649"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q57675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="11427726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="701" mass="80132" checksum="FBC53187B01B31EA" modified="2000-05-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX07</accession>
    <name>PYRI_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Aspartate carbamoyltransferase regulatory chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">pyrI</name>
        <name type="ordered locus">SSO0613</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in allosteric regulation of aspartate carbamoyltransferase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Contains catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PyrI family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40924" version="1"/>
        </conflict>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAB57687" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57687.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40924.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90208">
        <property type="entry name" value="E90208"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991142.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX07"/>
    <dbReference type="SMR" id="Q9UX07"/>
    <dbReference type="FunCoup" id="Q9UX07">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0613"/>
    <dbReference type="PaxDb" id="273057-SSO0613"/>
    <dbReference type="EnsemblBacteria" id="AAK40924">
        <property type="protein sequence ID" value="AAK40924"/>
        <property type="gene ID" value="SSO0613"/>
    </dbReference>
    <dbReference type="GeneID" id="1454891"/>
    <dbReference type="GeneID" id="44129615"/>
    <dbReference type="KEGG" id="sso:SSO0613"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.621"/>
    <dbReference type="eggNOG" id="arCOG04229">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128576_0_0_2"/>
    <dbReference type="InParanoid" id="Q9UX07"/>
    <dbReference type="PhylomeDB" id="Q9UX07"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009347">
        <property type="term" value="C:aspartate carbamoyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.20">
        <property type="entry name" value="Aspartate carbamoyltransferase regulatory subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.140">
        <property type="entry name" value="Aspartate carbamoyltransferase regulatory subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00002">
        <property type="entry name" value="Asp_carb_tr_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020545">
        <property type="entry name" value="Asp_carbamoyltransf_reg_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002801">
        <property type="entry name" value="Asp_carbamoylTrfase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020542">
        <property type="entry name" value="Asp_carbamoyltrfase_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036792">
        <property type="entry name" value="Asp_carbatrfase_reg_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036793">
        <property type="entry name" value="Asp_carbatrfase_reg_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00240">
        <property type="entry name" value="ATCase_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805:SF1">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01948">
        <property type="entry name" value="PyrI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02748">
        <property type="entry name" value="PyrI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57825">
        <property type="entry name" value="Aspartate carbamoyltransferase, Regulatory-chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54893">
        <property type="entry name" value="Aspartate carbamoyltransferase, Regulatory-chain, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000142343" description="Aspartate carbamoyltransferase regulatory chain">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="159" mass="17982" checksum="CE20EDD33442EDC3" modified="2002-08-02" version="2">MISSSKRDELIVSKIRNGTVIDHIPAGRALAVLRILGIRGSEGYRVALVMNVESKKIGRKDIVKIEDRVIDEKEASLITLIAPSATINIIRDYVVTEKRHLEVPKQIRGLIKCPNPQCITNNDVEAESRFITISIKPLKLKCKYCEMYITEDDVIRQIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-02-21" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q9Y8K9</accession>
    <name>Y428_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein SSO0428</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0428</name>
        <name type="ORF">C41_039</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AJ243289">
        <property type="protein sequence ID" value="CAB46015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90187">
        <property type="entry name" value="B90187"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9Y8K9"/>
    <dbReference type="SMR" id="Q9Y8K9"/>
    <dbReference type="STRING" id="273057.SSO0428"/>
    <dbReference type="PaxDb" id="273057-SSO0428"/>
    <dbReference type="EnsemblBacteria" id="AAK40753">
        <property type="protein sequence ID" value="AAK40753"/>
        <property type="gene ID" value="SSO0428"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0428"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.420"/>
    <dbReference type="eggNOG" id="arCOG05939">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172986_0_0_2"/>
    <dbReference type="InParanoid" id="Q9Y8K9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058494">
        <property type="entry name" value="DUF8181"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26552">
        <property type="entry name" value="DUF8181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000206881" description="Uncharacterized protein SSO0428">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <sequence length="111" mass="13123" checksum="00EEAE5B89123AD9" modified="1999-11-01" version="1">MMSEVKFNAYSKAIMEFEDEIKRIKKEIADDSKRLITLSDSYLSELRATAEKSLGDVQKLLNDEKKRQLDEIRNKYISEREKQLEEIRKEAEKNIDKVVNEVIRQLLGVFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="139" xmlns="https://uniprot.org/uniprot">
    <accession>O33772</accession>
    <name>HIS4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber>5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hisA</name>
        <name type="ordered locus">SSO0594</name>
        <name type="ORF">C08_055</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="J. Bacteriol." volume="179" first="4429" last="4432">
            <title>Evolutionary analysis of the hisCGABdFDEHI gene cluster from the archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Sensen C.W."/>
                <person name="Doolittle W.F."/>
                <person name="Brown J.R."/>
            </authorList>
            <dbReference type="PubMed" id="9209067"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16"/>
    <dbReference type="EMBL" id="U82227">
        <property type="protein sequence ID" value="AAB63020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90206">
        <property type="entry name" value="C90206"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991113.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O33772"/>
    <dbReference type="SMR" id="O33772"/>
    <dbReference type="FunCoup" id="O33772">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0594"/>
    <dbReference type="PaxDb" id="273057-SSO0594"/>
    <dbReference type="EnsemblBacteria" id="AAK40906">
        <property type="protein sequence ID" value="AAK40906"/>
        <property type="gene ID" value="SSO0594"/>
    </dbReference>
    <dbReference type="GeneID" id="1454873"/>
    <dbReference type="GeneID" id="44129596"/>
    <dbReference type="KEGG" id="sso:SSO0594"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.602"/>
    <dbReference type="eggNOG" id="arCOG00618">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_1_1_2"/>
    <dbReference type="InParanoid" id="O33772"/>
    <dbReference type="PhylomeDB" id="O33772"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04732">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01014">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023016">
        <property type="entry name" value="HisA/PriA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010113">
        <property type="entry name" value="PRK13586.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090:SF2">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142103" description="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="232" mass="25899" checksum="4E8C4D1CC7549186" modified="1998-01-01" version="1">MNEIIPSIDISFGKAVKRIRGVRGTGLVLGNPIELANKLYNEGYSRIHVVDLDAAEGVGNNEMYIKEICKIGFDWIQVGGGIRDVEKAKRLISLDVNALIFSTIVFTNFNLFYNIVREIGNNRVIVSIDYDDTKRVLISGWKERSMEIIDGIKKVNELELLGIILTYVTNEGTVKGIDYSVKDYAKLIRGVKEYAGGVSSDSDITFLKNVGFDYIIVGMAFYLNKIRGTNVV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2026-01-28" version="150" xmlns="https://uniprot.org/uniprot">
    <accession>O33775</accession>
    <name>HISX_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Histidinol dehydrogenase</fullName>
            <shortName>HDH</shortName>
            <ecNumber>1.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hisD</name>
        <name type="ordered locus">SSO0599</name>
        <name type="ORF">C08_051</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="J. Bacteriol." volume="179" first="4429" last="4432">
            <title>Evolutionary analysis of the hisCGABdFDEHI gene cluster from the archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Sensen C.W."/>
                <person name="Doolittle W.F."/>
                <person name="Brown J.R."/>
            </authorList>
            <dbReference type="PubMed" id="9209067"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>L-histidinol + 2 NAD(+) + H2O = L-histidine + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.23"/>
    <dbReference type="EMBL" id="U82227">
        <property type="protein sequence ID" value="AAB63023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90206">
        <property type="entry name" value="F90206"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991117.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O33775"/>
    <dbReference type="SMR" id="O33775"/>
    <dbReference type="FunCoup" id="O33775">
        <property type="interactions" value="261"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0599"/>
    <dbReference type="PaxDb" id="273057-SSO0599"/>
    <dbReference type="EnsemblBacteria" id="AAK40909">
        <property type="protein sequence ID" value="AAK40909"/>
        <property type="gene ID" value="SSO0599"/>
    </dbReference>
    <dbReference type="GeneID" id="1454876"/>
    <dbReference type="GeneID" id="44129599"/>
    <dbReference type="KEGG" id="sso:SSO0599"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.605"/>
    <dbReference type="eggNOG" id="arCOG04352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006732_3_0_2"/>
    <dbReference type="InParanoid" id="O33775"/>
    <dbReference type="PhylomeDB" id="O33775"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000001">
        <property type="entry name" value="Histidinol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000026">
        <property type="entry name" value="Histidinol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022695">
        <property type="entry name" value="Histidinol_DH_monofunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256:SF2">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS TRIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="HISTIDINOL DEHYDROGENASE HDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000099">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000135906" description="Histidinol dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>substrate</name>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>substrate</name>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="247"/>
        </location>
        <ligand>
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            <position position="247"/>
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        <ligand>
            <name>Zn(2+)</name>
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        <ligand>
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            <position position="384"/>
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            <position position="389"/>
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        <ligand>
            <name>Zn(2+)</name>
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        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="398" mass="43635" checksum="B989B35227ED564B" modified="1998-01-01" version="1">MISYSLPNERPNDFSRVIPVVKDIIESVKTKGDNALYELTEKLDKVKIDNIKAREEELKTQASKLDPKVKQAIDTAYEQLKAFHEMLVPPNIGGGYQGISFGVIWRSIEKIGIYVPSGKYSYPSTLLMAGIPAKVAKVKEIYVASPPTQEGTVNPALAYVAIKLGVNEVYKIGGAQAIAALAFGTESVKKVYKIVGPGNVYVQAAKYLVSSVVGIDGIEGPTELVIIADETAKAEYVALDMKAQAEHGPDTYIVLLSNDDELIRRVEEKIKNDKKIYYIIKTKNLDEAIEIANKIAPEHLSLYVKDAYTLMDKIVNAGAISLGNTPPAIIDYVAGPNHILPTNGWAKIRGGITVYDFIKPTMYANVRDINKQLLEASISLANYEGFIIHGKSIGARYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>P60849</accession>
    <accession>P74761</accession>
    <accession>Q97ZF6</accession>
    <name>ALBA1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA/RNA-binding protein Alba 1</fullName>
            <ecNumber evidence="13">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">albA1</name>
        <name evidence="14 15" type="synonym">sso10b</name>
        <name type="ordered locus">SSO0962</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2000-10" db="EMBL/GenBank/DDBJ databases">
            <title>Thermostable DNA-binding proteins from extremophilic Archaea, characterization and heterologous expression of 10kDa proteins from Sulfolobus solfataricus and Sulfolobus shibatae.</title>
            <authorList>
                <person name="Wu X.Q."/>
                <person name="Oppermann M."/>
                <person name="Knapp S."/>
                <person name="Berndt K.D."/>
                <person name="Bergman T."/>
                <person name="Joernvall H."/>
                <person name="Oppermann U.C.T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2002" name="Science" volume="296" first="148" last="151">
            <title>The interaction of Alba, a conserved archaeal chromatin protein, with Sir2 and its regulation by acetylation.</title>
            <authorList>
                <person name="Bell S.D."/>
                <person name="Botting C.H."/>
                <person name="Wardleworth B.N."/>
                <person name="Jackson S.P."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="11935028"/>
            <dbReference type="DOI" id="10.1126/science.1070506"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>ACETYLATION AT SER-2 AND LYS-16</scope>
        <scope>MASS SPECTROMETRY</scope>
        <scope>MUTAGENESIS OF LYS-16 AND LYS-17</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="16" key="4">
        <citation type="journal article" date="2005" name="J. Biol. Chem." volume="280" first="21122" last="21128">
            <title>Sir2 and the acetyltransferase, Pat, regulate the archaeal chromatin protein, Alba.</title>
            <authorList>
                <person name="Marsh V.L."/>
                <person name="Peak-Chew S.Y."/>
                <person name="Bell S.D."/>
            </authorList>
            <dbReference type="PubMed" id="15824122"/>
            <dbReference type="DOI" id="10.1074/jbc.m501280200"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>ACETYLATION AT LYS-16</scope>
        <scope>MUTAGENESIS OF LYS-16 AND LYS-17</scope>
    </reference>
    <reference evidence="16" key="5">
        <citation type="journal article" date="2007" name="Mol. Microbiol." volume="64" first="1540" last="1548">
            <title>An acetylase with relaxed specificity catalyses protein N-terminal acetylation in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Mackay D.T."/>
                <person name="Botting C.H."/>
                <person name="Taylor G.L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="17511810"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.2007.05752.x"/>
        </citation>
        <scope>ACETYLATION AT SER-2</scope>
        <scope>MUTAGENESIS OF SER-2</scope>
    </reference>
    <reference evidence="16" key="6">
        <citation type="journal article" date="2010" name="Biochem. J." volume="427" first="49" last="55">
            <title>Dimer-dimer stacking interactions are important for nucleic acid binding by the archaeal chromatin protein Alba.</title>
            <authorList>
                <person name="Jelinska C."/>
                <person name="Petrovic-Stojanovska B."/>
                <person name="Ingledew W.J."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="20082605"/>
            <dbReference type="DOI" id="10.1042/bj20091841"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF PHE-60</scope>
    </reference>
    <reference evidence="16" key="7">
        <citation type="journal article" date="2012" name="Nat. Commun." volume="3" first="1328" last="1328">
            <title>Alba shapes the archaeal genome using a delicate balance of bridging and stiffening the DNA.</title>
            <authorList>
                <person name="Laurens N."/>
                <person name="Driessen R.P."/>
                <person name="Heller I."/>
                <person name="Vorselen D."/>
                <person name="Noom M.C."/>
                <person name="Hol F.J."/>
                <person name="White M.F."/>
                <person name="Dame R.T."/>
                <person name="Wuite G.J."/>
            </authorList>
            <dbReference type="PubMed" id="23271660"/>
            <dbReference type="DOI" id="10.1038/ncomms2330"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH ALBA2</scope>
        <scope>MUTAGENESIS OF PHE-60</scope>
    </reference>
    <reference evidence="16" key="8">
        <citation type="journal article" date="2018" name="Mol. Microbiol." volume="109" first="192" last="208">
            <title>Insights into the post-translational modifications of archaeal Sis10b (Alba): lysine-16 is methylated, not acetylated, and this does not regulate transcription or growth.</title>
            <authorList>
                <person name="Cao J."/>
                <person name="Wang Q."/>
                <person name="Liu T."/>
                <person name="Peng N."/>
                <person name="Huang L."/>
            </authorList>
            <dbReference type="PubMed" id="29679495"/>
            <dbReference type="DOI" id="10.1111/mmi.13973"/>
        </citation>
        <scope>ACETYLATION AT SER-2; LYS-48; LYS-64 AND LYS-68</scope>
        <scope>DEAMIDATION AT ASN-31; GLN-32 AND ASN-58</scope>
        <scope>METHYLATION AT LYS-16; LYS-40; ASP-51; LYS-64; GLU-69; GLN-75; ASP-81 AND LYS-97</scope>
    </reference>
    <reference evidence="16" key="9">
        <citation type="journal article" date="2022" name="Biomolecules" volume="12" first="0" last="0">
            <title>Interplay between Alba and Cren7 Regulates Chromatin Compaction in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Cajili M.K.M."/>
                <person name="Prieto E.I."/>
            </authorList>
            <dbReference type="PubMed" id="35454068"/>
            <dbReference type="DOI" id="10.3390/biom12040481"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH ALBA2</scope>
    </reference>
    <reference evidence="16" key="10">
        <citation type="journal article" date="2023" name="Cell Rep." volume="42" first="112292" last="112292">
            <title>Nuclease activity of Plasmodium falciparum Alba family protein PfAlba3.</title>
            <authorList>
                <person name="Banerjee C."/>
                <person name="Nag S."/>
                <person name="Goyal M."/>
                <person name="Saha D."/>
                <person name="Siddiqui A.A."/>
                <person name="Mazumder S."/>
                <person name="Debsharma S."/>
                <person name="Pramanik S."/>
                <person name="Bandyopadhyay U."/>
            </authorList>
            <dbReference type="PubMed" id="36947546"/>
            <dbReference type="DOI" id="10.1016/j.celrep.2023.112292"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <reference evidence="17 18" key="11">
        <citation type="journal article" date="2002" name="EMBO J." volume="21" first="4654" last="4662">
            <title>Structure of Alba: an archaeal chromatin protein modulated by acetylation.</title>
            <authorList>
                <person name="Wardleworth B.N."/>
                <person name="Russell R.J.M."/>
                <person name="Bell S.D."/>
                <person name="Taylor G.L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="12198167"/>
            <dbReference type="DOI" id="10.1093/emboj/cdf465"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS)</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="19" key="12">
        <citation type="journal article" date="2005" name="Structure" volume="13" first="963" last="971">
            <title>Obligate heterodimerization of the archaeal Alba2 protein with Alba1 provides a mechanism for control of DNA packaging.</title>
            <authorList>
                <person name="Jelinska C."/>
                <person name="Conroy M.J."/>
                <person name="Craven C.J."/>
                <person name="Hounslow A.M."/>
                <person name="Bullough P.A."/>
                <person name="Waltho J.P."/>
                <person name="Taylor G.L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="16004869"/>
            <dbReference type="DOI" id="10.1016/j.str.2005.04.016"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS)</scope>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH ALBA2</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 4 5 7 9 10 12 13">Binds double-stranded DNA tightly but without sequence specificity (PubMed:11935028, PubMed:12198167, PubMed:20082605). Involved in DNA compaction (PubMed:16004869, PubMed:23271660, PubMed:35454068). Possesses DNA endonuclease activity (PubMed:36947546). Prevents transcription after DNA binding (PubMed:11935028). Binds single-stranded DNA and RNA in vitro (PubMed:20082605). Binds rRNA and mRNA in vivo (By similarity). May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes (By similarity). Binds double-stranded RNA (dsRNA) and exhibits RNA chaperone activity (By similarity). Required for normal growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5 7 9 10 12">Homodimer; homodimers assemble cooperatively on DNA with consecutive dimers binding end-to-end (PubMed:12198167, PubMed:20082605, PubMed:23271660). May form higher order oligomers, e.g. homotetramers (By similarity). Interacts with Alba 2; heterodimers lack cooperative DNA-binding behavior and result in more compact chromatin structures compared to Alba 1 homodimers (PubMed:16004869, PubMed:23271660, PubMed:35454068).</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-9021190">
            <id>P60849</id>
        </interactant>
        <interactant intactId="EBI-9021190">
            <id>P60849</id>
            <label>albA1</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>2</experiments>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-9021190">
            <id>P60849</id>
        </interactant>
        <interactant intactId="EBI-9021196">
            <id>Q97ZF4</id>
            <label>albA2</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>6</experiments>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 6">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1 4 6">Acetylated (PubMed:11935028). Acetylation at Lys-16 by the Pat acetylase decreases DNA-binding affinity (PubMed:15824122). Deacetylation at Lys-16 by the CobB deacetylase increases DNA-binding affinity (PubMed:11935028). Acetylation at Ser-2 is involved in the regulation of the turnover of the protein.</text>
    </comment>
    <comment type="mass spectrometry" mass="10538.0" method="MALDI" evidence="4"/>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the histone-like Alba family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4 6 11">Acetylation at Lys-16 is described as a mechanism of Alba 1 activity regulation (PubMed:11935028, PubMed:15824122). However, in one of the reported experiments, no acetylation at Lys-16 was detected (PubMed:29679495).</text>
    </comment>
    <comment type="sequence caution" evidence="16">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK41236" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="13"/>
    <dbReference type="EMBL" id="AJ298830">
        <property type="protein sequence ID" value="CAC12668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41236.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90247">
        <property type="entry name" value="E90247"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992406.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1H0X">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A/B=1-97"/>
    </dbReference>
    <dbReference type="PDB" id="1H0Y">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A=1-97"/>
    </dbReference>
    <dbReference type="PDB" id="2BKY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A/B=1-97"/>
    </dbReference>
    <dbReference type="PDB" id="4R3L">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.84 A"/>
        <property type="chains" value="B=2-7"/>
    </dbReference>
    <dbReference type="PDBsum" id="1H0X"/>
    <dbReference type="PDBsum" id="1H0Y"/>
    <dbReference type="PDBsum" id="2BKY"/>
    <dbReference type="PDBsum" id="4R3L"/>
    <dbReference type="AlphaFoldDB" id="P60849"/>
    <dbReference type="BMRB" id="P60849"/>
    <dbReference type="SMR" id="P60849"/>
    <dbReference type="DIP" id="DIP-48444N"/>
    <dbReference type="FunCoup" id="P60849">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="IntAct" id="P60849">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0962"/>
    <dbReference type="iPTMnet" id="P60849"/>
    <dbReference type="PaxDb" id="273057-SSO0962"/>
    <dbReference type="EnsemblBacteria" id="AAK41236">
        <property type="protein sequence ID" value="AAK41236"/>
        <property type="gene ID" value="SSO0962"/>
    </dbReference>
    <dbReference type="GeneID" id="42364119"/>
    <dbReference type="GeneID" id="84061577"/>
    <dbReference type="KEGG" id="sso:SSO0962"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.960"/>
    <dbReference type="eggNOG" id="arCOG01753">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110989_1_0_2"/>
    <dbReference type="InParanoid" id="P60849"/>
    <dbReference type="PhylomeDB" id="P60849"/>
    <dbReference type="EvolutionaryTrace" id="P60849"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000353"/>
        <property type="project" value="IntAct"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.20:FF:000008">
        <property type="entry name" value="DNA/RNA-binding protein Alba 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01122">
        <property type="entry name" value="AlbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013795">
        <property type="entry name" value="DNA/RNA-bd_Alba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00285">
        <property type="entry name" value="DNA-binding protein Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003088">
        <property type="entry name" value="PRK04015.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028732">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0007">Acetylation</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0158">Chromosome</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0226">DNA condensation</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="initiator methionine" description="Removed" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000151712" description="DNA/RNA-binding protein Alba 1">
        <location>
            <begin position="2"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>RNA</name>
            <dbReference type="ChEBI" id="CHEBI:33697"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>RNA</name>
            <dbReference type="ChEBI" id="CHEBI:33697"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>RNA</name>
            <dbReference type="ChEBI" id="CHEBI:33697"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>RNA</name>
            <dbReference type="ChEBI" id="CHEBI:33697"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>RNA</name>
            <dbReference type="ChEBI" id="CHEBI:33697"/>
        </ligand>
    </feature>
    <feature type="site" description="Not acetylated by pat acetylase" evidence="6">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="modified residue" description="N-acetylserine; by ard1 acetylase" evidence="4 8 11">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6,N6,N6-trimethyllysine; alternate" evidence="11">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6,N6-dimethyllysine; alternate" evidence="11">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine; by pat acetylase; alternate" evidence="4 6">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine; alternate" evidence="11">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="modified residue" description="Deamidated asparagine; partial" evidence="11">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="modified residue" description="Deamidated glutamine; partial" evidence="11">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine; partial" evidence="11">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine; partial" evidence="11">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="modified residue" description="Aspartate methyl ester; partial" evidence="11">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="modified residue" description="Deamidated asparagine; partial" evidence="11">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine; alternate; partial" evidence="11">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine; alternate; partial" evidence="11">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine; partial" evidence="11">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="modified residue" description="Glutamate methyl ester (Glu); partial" evidence="11">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine; partial" evidence="11">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="modified residue" description="Aspartate methyl ester; partial" evidence="11">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine; partial" evidence="11">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Decreases acetylation." evidence="8">
        <original>S</original>
        <variation>A</variation>
        <variation>E</variation>
        <variation>G</variation>
        <variation>L</variation>
        <variation>T</variation>
        <variation>P</variation>
        <variation>V</variation>
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Decreases DNA binding affinity." evidence="4">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Decreases DNA binding affinity. Unable to repress transcription. Abolishes acetylation." evidence="4 6">
        <original>K</original>
        <variation>E</variation>
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Decreases DNA binding affinity. Unable to repress transcription." evidence="4">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Decreases DNA binding affinity. No significant effect on acetylation." evidence="4 6">
        <original>K</original>
        <variation>E</variation>
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Decreases DNA binding affinity and cooperative side-by-side binding between homodimers." evidence="9 10">
        <original>F</original>
        <variation>A</variation>
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="strand" evidence="20">
        <location>
            <begin position="11"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="helix" evidence="20">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="strand" evidence="20">
        <location>
            <begin position="36"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="helix" evidence="20">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="turn" evidence="20">
        <location>
            <begin position="62"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="strand" evidence="20">
        <location>
            <begin position="65"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="strand" evidence="20">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="F0NHH1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P60848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="11935028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="12198167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="15824122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="16004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="8">
        <source>
            <dbReference type="PubMed" id="17511810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="9">
        <source>
            <dbReference type="PubMed" id="20082605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="10">
        <source>
            <dbReference type="PubMed" id="23271660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="11">
        <source>
            <dbReference type="PubMed" id="29679495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="12">
        <source>
            <dbReference type="PubMed" id="35454068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="13">
        <source>
            <dbReference type="PubMed" id="36947546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="14">
        <source>
            <dbReference type="PubMed" id="29679495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="15">
        <source>
            <dbReference type="PubMed" id="36947546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="16"/>
    <evidence type="ECO:0007744" key="17">
        <source>
            <dbReference type="PDB" id="1H0X"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="18">
        <source>
            <dbReference type="PDB" id="1H0Y"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="19">
        <source>
            <dbReference type="PDB" id="2BKY"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="20">
        <source>
            <dbReference type="PDB" id="2BKY"/>
        </source>
    </evidence>
    <sequence length="97" mass="10585" checksum="22C1BC9CCDB6B013" modified="2007-01-23" version="2">MSSGTPTPSNVVLIGKKPVMNYVLAALTLLNQGVSEIVIKARGRAISKAVDTVEIVRNRFLPDKIEIKEIRVGSQVVTSQDGRQSRVSTIEIAIRKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>P95871</accession>
    <name>TRI_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Tricorn protease homolog</fullName>
            <ecNumber>3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">tri</name>
        <name type="ordered locus">SSO2098</name>
        <name type="ORF">C06024</name>
        <name type="ORF">C06_007</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Degrades oligopeptides in a sequential manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S41B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-"/>
    <dbReference type="EMBL" id="Y08256">
        <property type="protein sequence ID" value="CAA69507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73091">
        <property type="entry name" value="S73091"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95871"/>
    <dbReference type="SMR" id="P95871"/>
    <dbReference type="STRING" id="273057.SSO2098"/>
    <dbReference type="MEROPS" id="S41.005"/>
    <dbReference type="PaxDb" id="273057-SSO2098"/>
    <dbReference type="EnsemblBacteria" id="AAK42276">
        <property type="protein sequence ID" value="AAK42276"/>
        <property type="gene ID" value="SSO2098"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2098"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2178"/>
    <dbReference type="eggNOG" id="arCOG03384">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005503_1_0_2"/>
    <dbReference type="InParanoid" id="P95871"/>
    <dbReference type="PhylomeDB" id="P95871"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd10828">
        <property type="entry name" value="cpPDZ_Tricorn-protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07562">
        <property type="entry name" value="Peptidase_S41_TRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.60:FF:000001">
        <property type="entry name" value="Tricorn protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.44">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.60">
        <property type="entry name" value="Tricorn protease N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005151">
        <property type="entry name" value="Tail-specific_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028204">
        <property type="entry name" value="Tricorn_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029414">
        <property type="entry name" value="Tricorn_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012393">
        <property type="entry name" value="Tricorn_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43253">
        <property type="entry name" value="TRICORN PROTEASE HOMOLOG 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43253:SF1">
        <property type="entry name" value="TRICORN PROTEASE HOMOLOG 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14685">
        <property type="entry name" value="PDZ_Tricorn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03572">
        <property type="entry name" value="Peptidase_S41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26550">
        <property type="entry name" value="Tricorn_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14684">
        <property type="entry name" value="Tricorn_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26549">
        <property type="entry name" value="Tricorn_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036421">
        <property type="entry name" value="Tricorn_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00245">
        <property type="entry name" value="TSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69304">
        <property type="entry name" value="Tricorn protease N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0720">Serine protease</keyword>
    <feature type="chain" id="PRO_0000207194" description="Tricorn protease homolog">
        <location>
            <begin position="1"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="region of interest" description="Six-bladed beta propeller" evidence="1">
        <location>
            <begin position="61"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Seven-bladed beta propeller" evidence="1">
        <location>
            <begin position="338"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-1" evidence="1">
        <location>
            <begin position="692"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="PDZ-like">
        <location>
            <begin position="771"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-2" evidence="1">
        <location>
            <begin position="865"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="756"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="974"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="1032"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="927"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer; via amide nitrogen" evidence="2">
        <location>
            <position position="975"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P96086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="1068" mass="123115" checksum="9F34FC13C9F3D4EC" modified="1997-05-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>P95914</accession>
    <name>Y2140_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Putative esterase SSO2140</fullName>
            <ecNumber>3.1.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2140</name>
        <name type="ORF">C01_016</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.-"/>
    <dbReference type="EMBL" id="Y08256">
        <property type="protein sequence ID" value="CAA69466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74052">
        <property type="entry name" value="S74052"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988336.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95914"/>
    <dbReference type="SMR" id="P95914"/>
    <dbReference type="FunCoup" id="P95914">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2140"/>
    <dbReference type="PaxDb" id="273057-SSO2140"/>
    <dbReference type="EnsemblBacteria" id="AAK42318">
        <property type="protein sequence ID" value="AAK42318"/>
        <property type="gene ID" value="SSO2140"/>
    </dbReference>
    <dbReference type="GeneID" id="1453645"/>
    <dbReference type="KEGG" id="sso:SSO2140"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2233"/>
    <dbReference type="eggNOG" id="arCOG00777">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_3_3_2"/>
    <dbReference type="InParanoid" id="P95914"/>
    <dbReference type="PhylomeDB" id="P95914"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000113">
        <property type="entry name" value="Thioesterase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660:SF1">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660">
        <property type="entry name" value="THIOESTERASE SUPERFAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156692" description="Putative esterase SSO2140">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="140" mass="15555" checksum="6E5EC61608033634" modified="1997-05-01" version="1">MEDPIKAIEEIFKKADQIFKFLDVKVINLEKGRAVVEIPYKEEFTRRGGVLHGGIIMSAIDITGGLAALTVNDAMDQVTQELKINFLEPMYKGPFTIEGKVLRKGSTVIVVEIEFKDADGKLGAKAIGSWYILRTKVQAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>P95937</accession>
    <name>YHIT_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized HIT-like protein SSO2163</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2163</name>
        <name type="ORF">C01_039</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="Y08256">
        <property type="protein sequence ID" value="CAA69441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74070">
        <property type="entry name" value="S74070"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992148.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95937"/>
    <dbReference type="SMR" id="P95937"/>
    <dbReference type="FunCoup" id="P95937">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2163"/>
    <dbReference type="PaxDb" id="273057-SSO2163"/>
    <dbReference type="EnsemblBacteria" id="AAK42339">
        <property type="protein sequence ID" value="AAK42339"/>
        <property type="gene ID" value="SSO2163"/>
    </dbReference>
    <dbReference type="GeneID" id="15299158"/>
    <dbReference type="KEGG" id="sso:SSO2163"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2256"/>
    <dbReference type="eggNOG" id="arCOG00419">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_3_2_2"/>
    <dbReference type="InParanoid" id="P95937"/>
    <dbReference type="PhylomeDB" id="P95937"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047627">
        <property type="term" value="F:adenylylsulfatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009150">
        <property type="term" value="P:purine ribonucleotide metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01277">
        <property type="entry name" value="HINT_subgroup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039384">
        <property type="entry name" value="HINT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47670">
        <property type="entry name" value="ADENYLYLSULFATASE HINT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47670:SF1">
        <property type="entry name" value="ADENYLYLSULFATASE HINT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000109835" description="Uncharacterized HIT-like protein SSO2163">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="HIT" evidence="1">
        <location>
            <begin position="3"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif">
        <location>
            <begin position="95"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <sequence length="139" mass="16011" checksum="D9745E8FFECBD50C" modified="1997-05-01" version="1">MCIFCNIVEGRDHGYIVYSNDRVVAFLDKFPITPGHTLVVPRTHYENFLEISEDVIPYLCTAVRKISIAVKKALKADGIRILTNIGKSAGQVVFHSHFHIVPTWSQDPDIMKDFVPRKEQSREYYEYVQKAIIETLKNI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1994-02-01" modified="2026-01-28" version="170" xmlns="https://uniprot.org/uniprot">
    <accession>Q06128</accession>
    <name>TRPE_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Anthranilate synthase component 1</fullName>
            <shortName>AS</shortName>
            <shortName>ASI</shortName>
            <ecNumber>4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">trpE</name>
        <name type="ordered locus">SSO0893</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1993" name="J. Bacteriol." volume="175" first="299" last="302">
            <title>Tryptophan biosynthesis genes trpEGC in the thermoacidophilic archaebacterium Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Tutino M.L."/>
                <person name="Scarano G."/>
                <person name="Marino G."/>
                <person name="Sannia G."/>
                <person name="Cubellis M.V."/>
            </authorList>
            <dbReference type="PubMed" id="8416906"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Biochem. Biophys. Res. Commun." volume="230" first="306" last="310">
            <title>Expression of Sulfolobus solfataricus trpE and trpG genes in E. coli.</title>
            <authorList>
                <person name="Tutino M.L."/>
                <person name="Tosco A."/>
                <person name="Marino G."/>
                <person name="Sannia G."/>
            </authorList>
            <dbReference type="PubMed" id="9016772"/>
            <dbReference type="DOI" id="10.1006/bbrc.1996.5951"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="1999" name="Proc. Natl. Acad. Sci. U.S.A." volume="96" first="9479" last="9484">
            <title>The crystal structure of anthranilate synthase from Sulfolobus solfataricus: functional implications.</title>
            <authorList>
                <person name="Knoechel T."/>
                <person name="Ivens A."/>
                <person name="Hester G."/>
                <person name="Gonzalez A."/>
                <person name="Bauerle R."/>
                <person name="Wilmanns M."/>
                <person name="Kirschner K."/>
                <person name="Jansonius J.N."/>
            </authorList>
            <dbReference type="PubMed" id="10449718"/>
            <dbReference type="DOI" id="10.1073/pnas.96.17.9479"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)</scope>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Cooperatively feedback inhibited by tryptophan.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the anthranilate synthase component I family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAA73379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="CAA90311.1"/>
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    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="H90239"/>
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    <dbReference type="PIR" id="JC5323">
        <property type="entry name" value="JC5323"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992309.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="PDB" id="1QDL">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A=1-421"/>
    </dbReference>
    <dbReference type="PDBsum" id="1QDL"/>
    <dbReference type="AlphaFoldDB" id="Q06128"/>
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    <dbReference type="DIP" id="DIP-6202N"/>
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        <property type="interactions" value="249"/>
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    <dbReference type="IntAct" id="Q06128">
        <property type="interactions" value="1"/>
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    <dbReference type="MINT" id="Q06128"/>
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        <property type="protein sequence ID" value="AAK41175"/>
        <property type="gene ID" value="SSO0893"/>
    </dbReference>
    <dbReference type="GeneID" id="1455142"/>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_006493_9_3_2"/>
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        <property type="reaction ID" value="UER00040"/>
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    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
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    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
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    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
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        <property type="entry name" value="Anthranilate_synth_I_arc_typ"/>
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        <property type="entry name" value="Chorismate_C"/>
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        <property type="entry name" value="PRK13574.1"/>
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        <property type="entry name" value="TrpE-arch"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANTHRANILATE SYNTHASE COMPONENT 1"/>
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        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0021">Allosteric enzyme</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
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        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
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        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
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    </feature>
    <feature type="binding site" evidence="1">
        <location>
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        <ligand>
            <name>chorismate</name>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="269"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="357"/>
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        <ligand>
            <name>chorismate</name>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="377"/>
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        <ligand>
            <name>chorismate</name>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="391"/>
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        <ligand>
            <name>chorismate</name>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="393"/>
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        <ligand>
            <name>chorismate</name>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="406"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA73379 and 2; CAA90311." evidence="3" ref="1 2">
        <original>A</original>
        <variation>R</variation>
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            <position position="337"/>
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    <feature type="strand" evidence="4">
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            <begin position="1"/>
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            <begin position="6"/>
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    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P00897"/>
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    <evidence type="ECO:0000305" key="3"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="421" mass="47652" checksum="BD49BD7771ECC6EE" modified="2001-06-20" version="2">MEVHPISEFASPFEVFKCIERDFKVAGLLESIGGPQYKARYSVIAWSTNGYLKIHDDPVNILNGYLKDLKLADIPGLFKGGMIGYISYDAVRFWEKIRDLKPAAEDWPYAEFFTPDNIIIYDHNEGKVYVNADLSSVGGCGDIGEFKVSFYDESLNKNSYERIVSESLEYIRSGYIFQVVLSRFYRYIFSGDPLRIYYNLRRINPSPYMFYLKFDEKYLIGSSPELLFRVQDNIVETYPIAGTRPRGADQEEDLKLELELMNSEKDKAEHLMLVDLARNDLGKVCVPGTVKVPELMYVEKYSHVQHIVSKVIGTLKKKYNALNVLSATFPAGTVSGAPKPMAMNIIETLEEYKRGPYAGAVGFISADGNAEFAIAIRTAFLNKELLRIHAGAGIVYDSNPESEYFETEHKLKALKTAIGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-02-05" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX62</accession>
    <name>Q7LX62_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74051">
        <property type="entry name" value="S74051"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX62"/>
    <dbReference type="STRING" id="273057.SSO2139"/>
    <dbReference type="PaxDb" id="273057-SSO2139"/>
    <dbReference type="EnsemblBacteria" id="AAK42317">
        <property type="protein sequence ID" value="AAK42317"/>
        <property type="gene ID" value="SSO2139"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2139"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2232"/>
    <dbReference type="eggNOG" id="arCOG03169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061108_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX62"/>
    <dbReference type="PhylomeDB" id="Q7LX62"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301:SF8">
        <property type="entry name" value="ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301">
        <property type="entry name" value="DNA-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="395" mass="45130" checksum="0DD8C3D80804FBA2" modified="2011-05-31" version="1">MGTHISRVPMKFIFGKPVDEPFDRENEIKQLVEMMNRRQPVAIIGVRRIGKTSVILKSLKIIETPKVYLSVEDFIEGKSFDLVSFLSLYSSLLITNTLNYVDPKRKISQIVKQKGKELLDKLRELIGYVKLSFNINLVEVEVFLNSVKRGGVKESIPQVLELPERLAEEFGLNTIVVAIDEFQYLKLASQNYPGIFHLLRSKWQFHNKVSYVISGSSVGLLEKIFYDKKEPFYQFFFPIYVKQFDKNTSFNFLKQGFRDEGKDYEDNALLLAIEELDGIPAWLNYFGLKSLQCNEVTSECAKKVIEDITFNDPIVRGIIQEEYNKLGKNARIILKYLAEKGGKGDLKGLELSRASINEGLKSLLGSGYIVKEERGKYAIIDPVIVKVLKSSTQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-03-27" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX75</accession>
    <name>Q7LX75_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74050">
        <property type="entry name" value="S74050"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX75"/>
    <dbReference type="STRING" id="273057.SSO2123"/>
    <dbReference type="PaxDb" id="273057-SSO2123"/>
    <dbReference type="EnsemblBacteria" id="AAK42302">
        <property type="protein sequence ID" value="AAK42302"/>
        <property type="gene ID" value="SSO2123"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2123"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2213"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_8_0_2"/>
    <dbReference type="InParanoid" id="Q7LX75"/>
    <dbReference type="PhylomeDB" id="Q7LX75"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="1">
        <location>
            <begin position="174"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="287" mass="34191" checksum="8A143C3741ED2AE5" modified="2011-05-31" version="1">MPTLGFRFRAYADEQTLRALKAQLKLACKIYNTLRWADIYFYQRDGKGLTQTELRQLALDLRKQDDEYKQLYSQVVQQIADRYYEAKKRFFEGLARFPKEKKPHKHYSLVYTQSGWKILHVREIRKGKKKLITLKLSNLGTFKVIIHRDFPLDKVKRVIVKLTRSERIYITFVVDHEFPKLPNTGKEVAIDVGVEKLLVTSDGEYFPNLRPLEKALWKVKHLHRELSRKKFLSHNWFKAKVKLARAYEHLKNLRTDLYMKLGKWFAEHYDVVVMEGIHAKQLVWVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX82</accession>
    <name>Q7LX82_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2114</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74026">
        <property type="entry name" value="S74026"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990146.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX82"/>
    <dbReference type="STRING" id="273057.SSO2114"/>
    <dbReference type="PaxDb" id="273057-SSO2114"/>
    <dbReference type="EnsemblBacteria" id="AAK42293">
        <property type="protein sequence ID" value="AAK42293"/>
        <property type="gene ID" value="SSO2114"/>
    </dbReference>
    <dbReference type="GeneID" id="1453620"/>
    <dbReference type="KEGG" id="sso:SSO2114"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2203"/>
    <dbReference type="eggNOG" id="arCOG00732">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142189_2_0_2"/>
    <dbReference type="InParanoid" id="Q7LX82"/>
    <dbReference type="PhylomeDB" id="Q7LX82"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027395">
        <property type="entry name" value="WH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37318">
        <property type="entry name" value="BSL7504 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37318:SF1">
        <property type="entry name" value="BSL7504 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13601">
        <property type="entry name" value="HTH_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="HTH arsR-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="103" mass="11862" checksum="FFF32849DFF87A6E" modified="2011-05-31" version="1">MSNEEERIKELIQFLNNRALNNSVRLAILIALYTFGQMTFSELLEYSRIPKSSLTMHLQILEEEGLAVSKKGFTVNGVRTFVRITDKGKETVKEYFKIIGDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX83</accession>
    <name>Q7LX83_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Metabolite permease, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2113</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAK42292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74025">
        <property type="entry name" value="S74025"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990144.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX83"/>
    <dbReference type="FunCoup" id="Q7LX83">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2113"/>
    <dbReference type="PaxDb" id="273057-SSO2113"/>
    <dbReference type="EnsemblBacteria" id="AAK42292">
        <property type="protein sequence ID" value="AAK42292"/>
        <property type="gene ID" value="SSO2113"/>
    </dbReference>
    <dbReference type="GeneID" id="1453619"/>
    <dbReference type="KEGG" id="sso:SSO2113"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2201"/>
    <dbReference type="eggNOG" id="arCOG01316">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018808_15_0_2"/>
    <dbReference type="InParanoid" id="Q7LX83"/>
    <dbReference type="PhylomeDB" id="Q7LX83"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050277">
        <property type="entry name" value="Sodium:Solute_Symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086:SF8">
        <property type="entry name" value="MONOCARBOXYLIC ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086">
        <property type="entry name" value="SODIUM/PROLINE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="421"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="450"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="488"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="526" mass="56211" checksum="2A662931D1FFE85A" modified="2011-05-31" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX90</accession>
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        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
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        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="S74018"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="PaxDb" id="273057-SSO2106"/>
    <dbReference type="EnsemblBacteria" id="AAK42285">
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        <property type="gene ID" value="SSO2106"/>
    </dbReference>
    <dbReference type="GeneID" id="1453612"/>
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    <dbReference type="PATRIC" id="fig|273057.12.peg.2192"/>
    <dbReference type="eggNOG" id="arCOG01191">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="15"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="132" mass="15055" checksum="6B656F1DB18883F1" modified="2011-05-31" version="1">MILNDMNFTTYRKLLTTAKDYLKASELSFEHNLYEASALNSEISAQLSLIFKLGIEPPRTHGIRELLSLIYAKLGDKRISDFTKENREKLIILENVRGKSQYGLPPVSRDEAEIALSTAQEILKLVESLWNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXE7</accession>
    <name>Q7LXE7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sugar phosphate nucleotydyl transferase</fullName>
            <ecNumber evidence="2">2.7.7.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0831</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AAK41126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="G90233"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="Q7LXE7">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0831"/>
    <dbReference type="PaxDb" id="273057-SSO0831"/>
    <dbReference type="EnsemblBacteria" id="AAK41126">
        <property type="protein sequence ID" value="AAK41126"/>
        <property type="gene ID" value="SSO0831"/>
    </dbReference>
    <dbReference type="GeneID" id="1455093"/>
    <dbReference type="KEGG" id="sso:SSO0831"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.846"/>
    <dbReference type="eggNOG" id="arCOG00667">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_1_2"/>
    <dbReference type="InParanoid" id="Q7LXE7"/>
    <dbReference type="PhylomeDB" id="Q7LXE7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04189">
        <property type="entry name" value="G1P_TT_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005908">
        <property type="entry name" value="G1P_thy_trans_l"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01208">
        <property type="entry name" value="rmlA_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42883">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE THYMIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42883:SF2">
        <property type="entry name" value="THYMIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="344" mass="37889" checksum="91B1145968D68607" modified="2011-05-31" version="1">MEAVILHGGQGTRLRPLTHTGPKQLIKVAGKPVSQWVLEQIRDAGIRDVIIVLGDNAPTRVVEYYGDGSKFGVRITYVYQGKARGLADAVYKVKDVVSDKFLVYLGDNLVPYDLSKFSSFKGSASILLAKVNNPNRFGVAVIKDGRVVKLIEKPKEQISDLALVGVYGFTREIFDVIESLKPSWRGELEITDAIQGLIDRGREVNYEIIQGWWKDTGTPKDILEANAFLLDRYAESRIEGVVDNSTVDGKVIIEKGAVIKNSVVRGPTYIGRDTKIINSYIGPFTSIGDQSEITDSEIEYSVILDGVKIRGVTLMDSLIGNNSTVEKGGRWQKLIIGENSSVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-03-27" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXH7</accession>
    <name>Q7LXH7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0796</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90229">
        <property type="entry name" value="G90229"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXH7"/>
    <dbReference type="STRING" id="273057.SSO0796"/>
    <dbReference type="PaxDb" id="273057-SSO0796"/>
    <dbReference type="EnsemblBacteria" id="AAK41094">
        <property type="protein sequence ID" value="AAK41094"/>
        <property type="gene ID" value="SSO0796"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0796"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.802"/>
    <dbReference type="eggNOG" id="arCOG08323">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102443_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXH7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="235" mass="27671" checksum="A4FA7B946A114115" modified="2011-05-31" version="1">MCRLISMSVELSTPFEQYYETLQRVKNRNFPLQYLEKMLDYLLEKYETNLFEIYEGILAIALNPEDLDIKMLLEEVRKIKEDFELYASLFENNTTIREYLEITINALGKLIDGLRKNEKKFYDEVNSTNPNFLLTTYYSLYLRYLTIVYAKLSKLNIQNDVKTKDSPFKNTLHFVNLIVGLLGMPILHYLKTKEVFPYISELSGVLLHTLTNEAPIPSRDSLGYYLFSRIHAKNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2021-09-29" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXK4</accession>
    <name>RPO4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo4</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo4</name>
        <name evidence="1 3" type="synonym">rpoF</name>
        <name type="ordered locus">SSO0751</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2008" name="Nature" volume="451" first="851" last="854">
            <title>The X-ray crystal structure of RNA polymerase from Archaea.</title>
            <authorList>
                <person name="Hirata A."/>
                <person name="Klein B.J."/>
                <person name="Murakami K.S."/>
            </authorList>
            <dbReference type="PubMed" id="18235446"/>
            <dbReference type="DOI" id="10.1038/nature06530"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF THE RNA POLYMERASE COMPLEX</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit is less well bound than the others.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 13-subunit RNA polymerase complex. Forms a stalk with Rpo7 that extends from the main structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic RPB4 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90223">
        <property type="entry name" value="G90223"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991330.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2PMZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="F/U=1-113"/>
    </dbReference>
    <dbReference type="PDB" id="3HKZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="F/R=1-113"/>
    </dbReference>
    <dbReference type="PDBsum" id="2PMZ"/>
    <dbReference type="PDBsum" id="3HKZ"/>
    <dbReference type="AlphaFoldDB" id="Q7LXK4"/>
    <dbReference type="SMR" id="Q7LXK4"/>
    <dbReference type="DIP" id="DIP-60648N"/>
    <dbReference type="FunCoup" id="Q7LXK4">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="IntAct" id="Q7LXK4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0751"/>
    <dbReference type="PaxDb" id="273057-SSO0751"/>
    <dbReference type="EnsemblBacteria" id="AAK41046">
        <property type="protein sequence ID" value="AAK41046"/>
        <property type="gene ID" value="SSO0751"/>
    </dbReference>
    <dbReference type="GeneID" id="1455013"/>
    <dbReference type="KEGG" id="sso:SSO0751"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.746"/>
    <dbReference type="eggNOG" id="arCOG01016">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165894_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXK4"/>
    <dbReference type="EvolutionaryTrace" id="Q7LXK4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00864">
        <property type="entry name" value="RNApol_arch_Rpo4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010924">
        <property type="entry name" value="Rpo4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011553">
        <property type="entry name" value="PRK14981.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39646:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT RPO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39646">
        <property type="entry name" value="RNA POLYMERASE RPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005053">
        <property type="entry name" value="RNA_pol_F_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000453811" description="DNA-directed RNA polymerase subunit Rpo4">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="3"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="16"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="23"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="32"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="37"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="49"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="56"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="66"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="72"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="80"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18235446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="11427726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="3HKZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <sequence length="113" mass="12811" checksum="DB070B2E7E4843DC" modified="2011-05-31" version="1">MSSVYIVEEHYIPYSVAKKLLTDVIRSGGSSNLLQRTYDYLNSVEKCDAESAQKVIEELSNIVSREDVRAILASICPTTSDEVRSILVMDTNKTYTSEDIQKIIDIIRKYIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXT1</accession>
    <name>Q7LXT1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cobalamin adenosyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO0588</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90205">
        <property type="entry name" value="F90205"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991107.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXT1"/>
    <dbReference type="SMR" id="Q7LXT1"/>
    <dbReference type="FunCoup" id="Q7LXT1">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0588"/>
    <dbReference type="PaxDb" id="273057-SSO0588"/>
    <dbReference type="EnsemblBacteria" id="AAK40901">
        <property type="protein sequence ID" value="AAK40901"/>
        <property type="gene ID" value="SSO0588"/>
    </dbReference>
    <dbReference type="GeneID" id="1454868"/>
    <dbReference type="KEGG" id="sso:SSO0588"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.597"/>
    <dbReference type="eggNOG" id="arCOG00489">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083486_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXT1"/>
    <dbReference type="PhylomeDB" id="Q7LXT1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:corrinoid adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1200.10">
        <property type="entry name" value="Cobalamin adenosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016030">
        <property type="entry name" value="CblAdoTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036451">
        <property type="entry name" value="CblAdoTrfase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029499">
        <property type="entry name" value="PduO-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00636">
        <property type="entry name" value="PduO_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213:SF0">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE MMAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01923">
        <property type="entry name" value="Cob_adeno_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89028">
        <property type="entry name" value="Cobalamin adenosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cobalamin adenosyltransferase-like" evidence="4">
        <location>
            <begin position="2"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="174" mass="20049" checksum="5E6893B5486B5A57" modified="2011-05-31" version="1">MFTRTGDDGNTNVISKRVGKDSPVVNLLGDIDELNSFIGLALTKIEWEDMQSDLMRVQTELFILGEEIIQDKGRINEETIKWLESRTVEYRKESGPVKLFVIPGGSEQASYLHVVRSIARRVERNAVAYSKELNFNKWIIVYLNRLSSLLFSMAIVANKRKNVKERIYDIGKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LYK2</accession>
    <name>Q7LYK2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator TrmB N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S73077">
        <property type="entry name" value="S73077"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990185.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LYK2"/>
    <dbReference type="STRING" id="273057.SSO2073"/>
    <dbReference type="PaxDb" id="273057-SSO2073"/>
    <dbReference type="EnsemblBacteria" id="AAK42255">
        <property type="protein sequence ID" value="AAK42255"/>
        <property type="gene ID" value="SSO2073"/>
    </dbReference>
    <dbReference type="GeneID" id="1453582"/>
    <dbReference type="KEGG" id="sso:SSO2073"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2150"/>
    <dbReference type="eggNOG" id="arCOG02037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237968_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LYK2"/>
    <dbReference type="PhylomeDB" id="Q7LYK2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="9"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="223" mass="25643" checksum="241885FE890621B0" modified="2011-05-31" version="1">MNSELEINLRKLGFSVYEAKVYLTLLNLCNSTMKELSENAEIPYQKVYEVVKSLEDKGLVRVIEGRPKKVKLIDPSISLKVYRDKIVSELDYAIGNVISYWSEKGKGEADRSLHIKGKTIVMRMIRELVDKSNKIKVVWDILPEWLIKILKKYKGNLTVITSSNNLSLNAEVKYVKNIKSKFIIFDDSVLVTFNDQDEIVVDSCRGCVLQAEEHFDLLTYKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-01-24" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TU7</accession>
    <name>Q97TU7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Second ORF in transposon ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1408</name>
        <name evidence="3" type="ordered locus">SSO1417</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90298">
        <property type="entry name" value="C90298"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TU7"/>
    <dbReference type="PaxDb" id="273057-SSO1408"/>
    <dbReference type="EnsemblBacteria" id="AAK41642">
        <property type="protein sequence ID" value="AAK41642"/>
        <property type="gene ID" value="SSO1408"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41650">
        <property type="protein sequence ID" value="AAK41650"/>
        <property type="gene ID" value="SSO1417"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1408"/>
    <dbReference type="KEGG" id="sso:SSO1417"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1429"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075002_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="14"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="229" mass="27140" checksum="EABD51CA3E219D83" modified="2001-10-01" version="1">MGEKEQEKAEGFLLYNWKRKGIKMRSLDLVYPLRLPLLVEIADLRSDSPSQFLLRSVMEVSQYMEIDYVVADAGFLNLGVIKEMPVKTIVRGKSNLKGFKELSNVPLVEKRYEVKDRVYVAYRVLKFEGLYYYDVVYVKGKPRHFMFVTNFEGDPYELAELYRLRWQVEEGFKVRKARIRYVRKLSNKIFLFLYYTVLDSAWNLVNHLLFNFKSTCKKGLSFDSFVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TV0</accession>
    <name>Q97TV0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease GajA/Old nuclease/RecF-like AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1710</name>
        <name evidence="3" type="ordered locus">SSO1789</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90340">
        <property type="entry name" value="H90340"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992506.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TV0"/>
    <dbReference type="PaxDb" id="273057-SSO1710"/>
    <dbReference type="EnsemblBacteria" id="AAK41915">
        <property type="protein sequence ID" value="AAK41915"/>
        <property type="gene ID" value="SSO1710"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41983">
        <property type="protein sequence ID" value="AAK41983"/>
        <property type="gene ID" value="SSO1789"/>
    </dbReference>
    <dbReference type="GeneID" id="1454762"/>
    <dbReference type="KEGG" id="sso:SSO1710"/>
    <dbReference type="KEGG" id="sso:SSO1789"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1759"/>
    <dbReference type="eggNOG" id="arCOG03240">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_586113_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041685">
        <property type="entry name" value="AAA_GajA/Old/RecF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051396">
        <property type="entry name" value="Bact_Antivir_Def_Nuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43581">
        <property type="entry name" value="ATP/GTP PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43581:SF4">
        <property type="entry name" value="ATP_GTP PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13175">
        <property type="entry name" value="AAA_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease GajA/Old nuclease/RecF-like AAA" evidence="1">
        <location>
            <begin position="204"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="464" mass="54020" checksum="D24C5C37F8A4E2BE" modified="2001-10-01" version="1">MEMAIYCGKTYSWANELCSKPLKEVKVVDYNSVVDWVNSQKERAFLIFGTDVIPYSLYEYPKIPVNETPLFKFMERGGVVIWTGDVPFFYIEKDGIKKELFSKGNPFPFKPISVMGHKPLSEKSENSIVGEMLKYDPKDSWRPVEPHPLLIPISIVKSHPYTLYSTWIYKYGKGAFVRLYDSPYVNTQYILSLPERLSSLGIGIRISNFRRFRDFKMIFPEFKIGVILGKNNVGKTTILEAIAMLGKNEDKIRKFRGNISTEIAETELFVNYTYYKAEFSYSQVNRSADVNVLLIYSHDIDFVIDDKVLPYVKSSLRKVTELLNSFDPNIFYVYLSSGNELRVLFNDRTDVSINELGYGYKSLLNFILLYVIYQPRIILIDDLEGFALHPDLLKMFYDLLLKIDVDLILITTQSSDIYAYLAEKRSDKVRFILINDDKYEVLTSEEVLDRLYYEDLRYTALKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TV1</accession>
    <name>Q97TV1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1439</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO7986</name>
        <name evidence="2" type="ordered locus">SSO8002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90298">
        <property type="entry name" value="D90298"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TV1"/>
    <dbReference type="PaxDb" id="273057-SSO7986"/>
    <dbReference type="EnsemblBacteria" id="AAK41643">
        <property type="protein sequence ID" value="AAK41643"/>
        <property type="gene ID" value="SSO7986"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK41651">
        <property type="protein sequence ID" value="AAK41651"/>
        <property type="gene ID" value="SSO8002"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7986"/>
    <dbReference type="KEGG" id="sso:SSO8002"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1430"/>
    <dbReference type="eggNOG" id="arCOG09924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2519932_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="84" mass="9470" checksum="CEFA8E9825CB92C0" modified="2001-10-01" version="1">MQTIQVSKTELKSLAITLATNNINVISQDLDLEIVKAAPSLLTGNRGKYYLKVVRQGEKVISKGQRTFKFYPIYREVKGEINVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TW1</accession>
    <name>Q97TW1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC774</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2242</name>
        <name evidence="2" type="ordered locus">SSO2898</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90468">
        <property type="entry name" value="H90468"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TW1"/>
    <dbReference type="STRING" id="273057.SSO2242"/>
    <dbReference type="PaxDb" id="273057-SSO2242"/>
    <dbReference type="EnsemblBacteria" id="AAK42409">
        <property type="protein sequence ID" value="AAK42409"/>
        <property type="gene ID" value="SSO2242"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK43007">
        <property type="protein sequence ID" value="AAK43007"/>
        <property type="gene ID" value="SSO2898"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2242"/>
    <dbReference type="KEGG" id="sso:SSO2898"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2336"/>
    <dbReference type="eggNOG" id="arCOG02133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139557_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="142" mass="16434" checksum="5F3D412E45F24F3F" modified="2001-10-01" version="1">MFSVISFWVSRGFFLVSKSWTNEVAALTQVILTLMEYINLKPRFHVREEIALSLASYLADLSSWRTTLFHFTLLYERLGSLRYAVSLSYAIDETKVTCVRGNYYVWVIREVVAKAISFFMVTSLRSGFHVVIMRGLRVSTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U09</accession>
    <name>Q97U09_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Second ORF in transposon ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO3217</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90507">
        <property type="entry name" value="C90507"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U09"/>
    <dbReference type="SMR" id="Q97U09"/>
    <dbReference type="PaxDb" id="273057-SSO3217"/>
    <dbReference type="EnsemblBacteria" id="AAK43314">
        <property type="protein sequence ID" value="AAK43314"/>
        <property type="gene ID" value="SSO3217"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3217"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3320"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_9_1_2"/>
    <dbReference type="InParanoid" id="Q97U09"/>
    <dbReference type="PhylomeDB" id="Q97U09"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="17"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="142"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK43314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="233" mass="26887" checksum="D85A7C86E9CA6603" modified="2001-10-01" version="1">MVVKLTRSERIYITFVVDHEFPKLPNTGKVVAIDVGVEKLLVTSDGEYFPNLRPYEKALWKVKHLHRELSRKKFLSNNWFKAKVKLARAYEYLKNLRTDLYMKLGKWFAEHYDVVVMEGIHAKQLVGKSLRSLRRRLSDVGFGELRDVLKYQLEKYGKKLILVNPAYTSKTCARCGYVKNDLSLSDRVFVCPNCGWIADRDYNASLNILKGAGSERPLVWSSALYQYSGKVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U17</accession>
    <name>Q97U17_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase, aldo/keto reductase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90506">
        <property type="entry name" value="B90506"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991668.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U17"/>
    <dbReference type="FunCoup" id="Q97U17">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3209"/>
    <dbReference type="PaxDb" id="273057-SSO3209"/>
    <dbReference type="EnsemblBacteria" id="AAK43305">
        <property type="protein sequence ID" value="AAK43305"/>
        <property type="gene ID" value="SSO3209"/>
    </dbReference>
    <dbReference type="GeneID" id="1453224"/>
    <dbReference type="KEGG" id="sso:SSO3209"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3311"/>
    <dbReference type="eggNOG" id="arCOG01619">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_3_2"/>
    <dbReference type="InParanoid" id="Q97U17"/>
    <dbReference type="PhylomeDB" id="Q97U17"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19072">
        <property type="entry name" value="AKR_AKR3F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43638:SF3">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43638">
        <property type="entry name" value="OXIDOREDUCTASE, ALDO/KETO REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="265" mass="30313" checksum="64F7B27EBDF14866" modified="2001-10-01" version="1">MRLCNKDVSQIGFGTWKIGGGYWNPDYSKDSHYIEILKYVISKGINVIDTAEMYGGGHSEELVGKAIENFDRDKIFIITKVWSNHLKYDDLIRSAKNSLKRLNAKYIDLYLIHWPNSSVPLEESLRAMEELVDQGIVNCIGVSNFDIKLLEETMSITKKYEITANEIEYNVENKSAEKDVIPFCERNNIKVIAYSPLARGNAKNNKILEEIGRKYNKTSVQVALNYLLRRSIPIPKASSKDHIDEILGALGWNLSDEDYERISKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U58</accession>
    <name>Q97U58_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO3164</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90501">
        <property type="entry name" value="A90501"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U58"/>
    <dbReference type="STRING" id="273057.SSO3164"/>
    <dbReference type="PaxDb" id="273057-SSO3164"/>
    <dbReference type="EnsemblBacteria" id="AAK43264">
        <property type="protein sequence ID" value="AAK43264"/>
        <property type="gene ID" value="SSO3164"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3164"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3269"/>
    <dbReference type="eggNOG" id="arCOG07342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1357967_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U58"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="208" mass="24509" checksum="FF879DE7015A6386" modified="2001-10-01" version="1">MIKSIILMPIYIYCIDKDKLIYCNNTGELYYVFEYTRNNELLLSKCRNSKCEQVDDVISELGKYRFANEIDNFDEIMGKIDEITSFLTKHNLKIYFIGDSSVLEAIYTPLLFYYKYFGLKEAKDKVNYVKSWLDKLILARRVLDKIGIMEFKSHMDTLDGRYAIWLNTEDESTSFISSEGDLVKCWISYNDCDLLIERKGKRICIKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U92</accession>
    <name>Q97U92_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SpoVT-AbrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO12018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90496">
        <property type="entry name" value="F90496"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989953.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U92"/>
    <dbReference type="STRING" id="273057.SSO12018"/>
    <dbReference type="PaxDb" id="273057-SSO12018"/>
    <dbReference type="EnsemblBacteria" id="AAK43229">
        <property type="protein sequence ID" value="AAK43229"/>
        <property type="gene ID" value="SSO12018"/>
    </dbReference>
    <dbReference type="GeneID" id="1453148"/>
    <dbReference type="KEGG" id="sso:SSO12018"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3234"/>
    <dbReference type="eggNOG" id="arCOG00818">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158484_9_2_2"/>
    <dbReference type="InParanoid" id="Q97U92"/>
    <dbReference type="PhylomeDB" id="Q97U92"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052975">
        <property type="entry name" value="Repressor-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860:SF7">
        <property type="entry name" value="TRANSCRIPTION REGULATOR, SPOVT_ABRB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="3"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="81" mass="9426" checksum="BDA92DA121F70CA8" modified="2001-10-01" version="1">MERRRVKVYKKGIIVIPKEIREKLGIKEGDIIELIVNGDRISIEKPLTLLDLFGIDGDEALEIAKEIIKERRKEVEKEIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U93</accession>
    <name>Q97U93_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90496">
        <property type="entry name" value="E90496"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989951.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U93"/>
    <dbReference type="SMR" id="Q97U93"/>
    <dbReference type="STRING" id="273057.SSO3128"/>
    <dbReference type="PaxDb" id="273057-SSO3128"/>
    <dbReference type="DNASU" id="1453147"/>
    <dbReference type="EnsemblBacteria" id="AAK43228">
        <property type="protein sequence ID" value="AAK43228"/>
        <property type="gene ID" value="SSO3128"/>
    </dbReference>
    <dbReference type="GeneID" id="1453147"/>
    <dbReference type="KEGG" id="sso:SSO3128"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3233"/>
    <dbReference type="eggNOG" id="arCOG02221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135601_2_1_2"/>
    <dbReference type="InParanoid" id="Q97U93"/>
    <dbReference type="PhylomeDB" id="Q97U93"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18689">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677:SF4">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="5"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="133" mass="15188" checksum="FC9ACDC9C4C55781" modified="2001-10-01" version="1">MRKKYVLDAGPLSLLFAGRKEIKKYFEEIYTGDAIIYMSEVNLAELLYIYILKKGKDVAIARHRYIRNSPIKVISPNERITENAALLKSKYSYLSLADAFLIATAKEVKGKVITTDEDIEKTKEVETIKIPLD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2014-06-11" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UL5</accession>
    <name>DMD_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Diphosphomevalonate decarboxylase</fullName>
            <shortName>DMD</shortName>
            <ecNumber>4.1.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2989</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2013" name="J. Biochem." volume="153" first="415" last="420">
            <title>Biochemical evidence supporting the presence of the classical mevalonate pathway in the thermoacidophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Nishimura H."/>
                <person name="Azami Y."/>
                <person name="Miyagawa M."/>
                <person name="Hashimoto C."/>
                <person name="Yoshimura T."/>
                <person name="Hemmi H."/>
            </authorList>
            <dbReference type="PubMed" id="23378249"/>
            <dbReference type="DOI" id="10.1093/jb/mvt006"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PATHWAY</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the decarboxylation of mevalonate 5-diphosphate (MVAPP) to isopentenyl diphosphate (IPP). Functions in the mevalonate (MVA) pathway leading to IPP, a key precursor for the biosynthesis of isoprenoid compounds such as archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(R)-5-diphosphomevalonate + ATP = isopentenyl diphosphate + ADP + phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:23732"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the diphosphomevalonate decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.33"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90479">
        <property type="entry name" value="G90479"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992640.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="4Z7C">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A=2-325"/>
    </dbReference>
    <dbReference type="PDB" id="4Z7Y">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B/C/D/E/F=2-325"/>
    </dbReference>
    <dbReference type="PDB" id="5GMD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.50 A"/>
        <property type="chains" value="A=1-325"/>
    </dbReference>
    <dbReference type="PDB" id="5GME">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A=1-325"/>
    </dbReference>
    <dbReference type="PDBsum" id="4Z7C"/>
    <dbReference type="PDBsum" id="4Z7Y"/>
    <dbReference type="PDBsum" id="5GMD"/>
    <dbReference type="PDBsum" id="5GME"/>
    <dbReference type="AlphaFoldDB" id="Q97UL5"/>
    <dbReference type="SMR" id="Q97UL5"/>
    <dbReference type="FunCoup" id="Q97UL5">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2989"/>
    <dbReference type="PaxDb" id="273057-SSO2989"/>
    <dbReference type="EnsemblBacteria" id="AAK43094">
        <property type="protein sequence ID" value="AAK43094"/>
        <property type="gene ID" value="SSO2989"/>
    </dbReference>
    <dbReference type="GeneID" id="1453013"/>
    <dbReference type="KEGG" id="sso:SSO2989"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3083"/>
    <dbReference type="eggNOG" id="arCOG02937">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040369_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UL5"/>
    <dbReference type="PhylomeDB" id="Q97UL5"/>
    <dbReference type="BRENDA" id="4.1.1.33">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00100"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97UL5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004163">
        <property type="term" value="F:diphosphomevalonate decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000087">
        <property type="entry name" value="Diphosphomevalonate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005935">
        <property type="entry name" value="Mev_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029765">
        <property type="entry name" value="Mev_diP_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053859">
        <property type="entry name" value="MVD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041431">
        <property type="entry name" value="Mvd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01240">
        <property type="entry name" value="mevDPdecarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977:SF3">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18376">
        <property type="entry name" value="MDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22700">
        <property type="entry name" value="MVD-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015950">
        <property type="entry name" value="Mev_P_decrbx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000429454" description="Diphosphomevalonate decarboxylase">
        <location>
            <begin position="1"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="18"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>(R)-5-diphosphomevalonate</name>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>(R)-5-diphosphomevalonate</name>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="139"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>(R)-5-diphosphomevalonate</name>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
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            <begin position="3"/>
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    </feature>
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        <location>
            <begin position="320"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="O23722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="23378249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="5GMD"/>
        </source>
    </evidence>
    <sequence length="325" mass="37028" checksum="01FB736096112177" modified="2001-10-01" version="1">MLKSVTVSAPSNIAVVKYWGKRGDERLNLPLNNSLSITLDDQLSVITKVTLNDKNIVIVNDRILSEDEMKEYAGRVLDTFKKIVGKEFHVKVESKSKFPINAGLASSAAGIAALAFSLNELLELNLKSEELSKIARLGSGSACRSMFGGFVVWNKGEREDGEDSYCYQIFRHDYWSELVDIIPILSEKEKKISSRKGMIRSAETSELMECRLKYIEKTFNEVIEAIRNRDEKKFYYLMMRHSNSMHAVILDSWPSFFYLNDTSIRIMEWIHDYGKAGYTFDAGPNPHIFTTERNIGDILEFLKSLEIKRIIVSKVGDGPKVLSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UQ5</accession>
    <name>Q97UQ5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposase ISC1173</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43053.1"/>
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        <property type="entry name" value="F90474"/>
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    <dbReference type="AlphaFoldDB" id="Q97UQ5"/>
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    <dbReference type="EnsemblBacteria" id="AAK43053">
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        <property type="gene ID" value="SSO2950"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2950"/>
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    <dbReference type="eggNOG" id="arCOG02133">
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    <dbReference type="HOGENOM" id="CLU_076276_0_1_2"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Transposase_27_IS1"/>
    </dbReference>
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        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293:SF1">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="42953" checksum="CA4AF700CF94E695" modified="2001-10-01" version="1">MNIFTMFSPTVGARHALARRLCFNSHLDHISPAEEYYSPQLINLYIGYEIFVRVTNMDLVTLAQLILMILRNLNLKPRKYELKDIALAAYSLGVQITKTGIPPSTLYYYLRKVGIRRRRESRPTCPSCNSNRVVKNGSSRGKAKYKCKACGRTFYDALKHRMDREQRERILKEYLNRMSMRAISRVEGKPLTTVYSLVRRVGIRAFTNLTILKGELKNFTAKFTVFDEFWTYFRVRHGVMREDLWIWTALADGVPFYEFGDRSYGTFRLGWLPRSEVNYTDYYCVYQVLDNRVAGKKYTYLVESHNSWCRLVRLARDTKAVTRSGRMAEYSLALLNVMYPHVFSRERTPLNEAYLKGVQYIRENLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>Q97US6</accession>
    <name>Q97US6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1058</fullName>
        </submittedName>
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        <lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
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    </dbReference>
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        <property type="entry name" value="C90471"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97US6"/>
    <dbReference type="PaxDb" id="273057-SSO11456"/>
    <dbReference type="EnsemblBacteria" id="AAK43026">
        <property type="protein sequence ID" value="AAK43026"/>
        <property type="gene ID" value="SSO11456"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11456"/>
    <dbReference type="HOGENOM" id="CLU_2893438_0_0_2"/>
    <dbReference type="InParanoid" id="Q97US6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="62" mass="6561" checksum="62BCBE0BB13F43FF" modified="2001-10-01" version="1">MKTPLSGVGLTEECVNYVVVLEPIVFSLPTSSSKPFSLYSLIALSKALFNSSISVVLIVSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97US7</accession>
    <name>Q97US7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2917</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90471">
        <property type="entry name" value="B90471"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924033.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97US7"/>
    <dbReference type="STRING" id="273057.SSO2917"/>
    <dbReference type="PaxDb" id="273057-SSO2917"/>
    <dbReference type="EnsemblBacteria" id="AAK43025">
        <property type="protein sequence ID" value="AAK43025"/>
        <property type="gene ID" value="SSO2917"/>
    </dbReference>
    <dbReference type="GeneID" id="1452944"/>
    <dbReference type="KEGG" id="sso:SSO2917"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3007"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154343_0_0_2"/>
    <dbReference type="InParanoid" id="Q97US7"/>
    <dbReference type="PhylomeDB" id="Q97US7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="147" mass="16794" checksum="7F7A74106FCB5211" modified="2001-10-01" version="1">MGFNDVFLLMGYTTSKLKNFTAFRKYKGSWGRKHGKSYFGFKVCNLVERRTNFVRDFKVGLANLSDLAFSFDNVKLMADRAWIFREDVLVKGVGSARLPVEGSGVEIREGKASSTTLRGVVIEVFFLNFYRDLEILSTRIRTKVLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UV2</accession>
    <name>Q97UV2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90467">
        <property type="entry name" value="G90467"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990806.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UV2"/>
    <dbReference type="STRING" id="273057.SSO2890"/>
    <dbReference type="PaxDb" id="273057-SSO2890"/>
    <dbReference type="DNASU" id="1452917"/>
    <dbReference type="EnsemblBacteria" id="AAK42998">
        <property type="protein sequence ID" value="AAK42998"/>
        <property type="gene ID" value="SSO2890"/>
    </dbReference>
    <dbReference type="GeneID" id="1452917"/>
    <dbReference type="KEGG" id="sso:SSO2890"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2978"/>
    <dbReference type="eggNOG" id="arCOG00505">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061385_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UV2"/>
    <dbReference type="PhylomeDB" id="Q97UV2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07726">
        <property type="entry name" value="ST1585-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037482">
        <property type="entry name" value="ST1585_MBL-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF4">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE MBLAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="267" mass="29258" checksum="9CAEC13F0A8B9361" modified="2001-10-01" version="1">MPCRGLHAIPAGPPEFPELATIYVVCGDKLNVMIDAGVTNSIMDSSFLEKLDLVILTHIHIDHIGLLPEILDRYKNAKVMIKNGFKKFLTTEDGVRRLNESSEKVLGDLYYIYGEFTKIDPDKVVEVEGGEMIDIGDGKTLKIIYTPGHAKHHISVVSDDILFTGDSGGAYFNGAVIPTTPPPLDYDNYVNSLRLQISLKPKVVGLAHGGLVSPNILEQHLDQMLGKESFDIGEIDIGGIGGEILRKQVEVNLRGLNEALGRNKKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V11</accession>
    <name>Q97V11_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Oxidoreductase molybdopterin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99460">
        <property type="entry name" value="A99460"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989015.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V11"/>
    <dbReference type="FunCoup" id="Q97V11">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2826"/>
    <dbReference type="PaxDb" id="273057-SSO2826"/>
    <dbReference type="EnsemblBacteria" id="AAK42936">
        <property type="protein sequence ID" value="AAK42936"/>
        <property type="gene ID" value="SSO2826"/>
    </dbReference>
    <dbReference type="GeneID" id="1452855"/>
    <dbReference type="KEGG" id="sso:SSO2826"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2911"/>
    <dbReference type="eggNOG" id="arCOG00266">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094953_1_1_2"/>
    <dbReference type="InParanoid" id="Q97V11"/>
    <dbReference type="PhylomeDB" id="Q97V11"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00321">
        <property type="entry name" value="SO_family_Moco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.420.10">
        <property type="entry name" value="Oxidoreductase, molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008335">
        <property type="entry name" value="Mopterin_OxRdtase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000572">
        <property type="entry name" value="OxRdtase_Mopterin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43032:SF4">
        <property type="entry name" value="OXIDOREDUCTASE MOLYBDOPTERIN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43032">
        <property type="entry name" value="PROTEIN-METHIONINE-SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00407">
        <property type="entry name" value="EUMOPTERIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56524">
        <property type="entry name" value="Oxidoreductase molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase molybdopterin-binding" evidence="1">
        <location>
            <begin position="52"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="209" mass="23903" checksum="91BFCB41D82165E7" modified="2001-10-01" version="1">MRLTNRRDFLKLLLISSSLLVLGRLSISEMQHAQNGLTPFGSWYVVQYSQIVPDIMLNNYVLTVDGEIENPLKLTYQDLLNMPSIEVKDTIQCVSDPYFLRANVLWKGIPLSYVIDMVKPKSNVIKVVGYGADGYTADLPIEKAKEPNVLIAYMADDKPLPQVHGYPVRLVVPGWWGYTYVKWLVRLHFTSKNILGYWESLGYPDYAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V34</accession>
    <name>Q97V34_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2798</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90456">
        <property type="entry name" value="H90456"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V34"/>
    <dbReference type="SMR" id="Q97V34"/>
    <dbReference type="STRING" id="273057.SSO2798"/>
    <dbReference type="PaxDb" id="273057-SSO2798"/>
    <dbReference type="EnsemblBacteria" id="AAK42911">
        <property type="protein sequence ID" value="AAK42911"/>
        <property type="gene ID" value="SSO2798"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2798"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2884"/>
    <dbReference type="eggNOG" id="arCOG00744">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1313174_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V34"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="209" mass="24063" checksum="E86BEBAC1AD69889" modified="2001-10-01" version="1">MMSIKILDDRDTLVLEFLVIYGYLTSYKLAKISDIPMATVWRILVNLKSLSLVAKQKKGFAITPRGLVFAYYLTKKENIRLEALQKLKEAWKYEGSVNEIRSFLEALNQFLKKYEISLISVCFNHPLSVISLMLPKAKELDEFSQRLLARFILRAFPTVVLPTGCKAIVSFDEKGEPYALAADCKDEGVHIFHKCPYINKYFSIEVRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V36</accession>
    <name>Q97V36_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2796</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90456">
        <property type="entry name" value="F90456"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991435.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V36"/>
    <dbReference type="STRING" id="273057.SSO2796"/>
    <dbReference type="PaxDb" id="273057-SSO2796"/>
    <dbReference type="EnsemblBacteria" id="AAK42909">
        <property type="protein sequence ID" value="AAK42909"/>
        <property type="gene ID" value="SSO2796"/>
    </dbReference>
    <dbReference type="GeneID" id="1452828"/>
    <dbReference type="KEGG" id="sso:SSO2796"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2882"/>
    <dbReference type="eggNOG" id="arCOG03428">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1014181_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V36"/>
    <dbReference type="PhylomeDB" id="Q97V36"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF4">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="98"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="166"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="274" mass="31765" checksum="9CDA34ACD7BE5705" modified="2001-10-01" version="1">MEDVIKQAEELLEKGRYNEVLKVLENFESVEVYLLRAEAYLRMNNSKAALVELDKGVQTFPFSYAILSAKAEVLENEGRLEEALENINKALEILPFSSEYRFIKARILFKLERYEEANIELTEVLRLNPNNVDARVMKAFCYYNMGLKLDALSEINRALNVKKEDSSLHELKGKIYFETGFYKLALSEFKIAAHYDPNNPDHYYNAAACYYNLKMYNDALTYMDLALSKGEKANYHILKALILKELNMEFSQEVEEAIKLDPSTKSIIENLFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V47</accession>
    <name>Q97V47_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2785</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90455">
        <property type="entry name" value="C90455"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923968.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V47"/>
    <dbReference type="PaxDb" id="273057-SSO2785"/>
    <dbReference type="EnsemblBacteria" id="AAK42898">
        <property type="protein sequence ID" value="AAK42898"/>
        <property type="gene ID" value="SSO2785"/>
    </dbReference>
    <dbReference type="GeneID" id="1452817"/>
    <dbReference type="KEGG" id="sso:SSO2785"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2869"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_2_2"/>
    <dbReference type="InParanoid" id="Q97V47"/>
    <dbReference type="PhylomeDB" id="Q97V47"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="175"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="301"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="399" mass="47004" checksum="1A26B262211F9DE6" modified="2001-10-01" version="1">MPTLGFRFRAHTDEQTLRALKAQLKLACEVYNTLRWADIYFYQRDGKGLTQTELRQLALDLRKQDDEYKQVYSQVVQQIADRYYEARQRFFEGLARFPKEKKPHKYYSLVYPQYGWKILQVREIRKGSKKKLITLKLSNLGVFKVIVHRDFPLDKVKRVVVKLTRSERIYITFVVEGYEFPKLPNTGKVVAIDVGVEKLLVTSDSEYFPNLRPLEKALWKVKHLHRELSRKKFLSNNWFKAKVKLARAYEHLKNLRTDLYMKLGKWFAEHYDVVVMEDIHVKQLIGKSLRSLRRRLSDVAFSELRDLIKYQLEKYGKKLILVNPAYTSKTCARCGYVKEDLSLSDRVFVCSNCGWIADRDYNASLNILRGSGSERSLVPVELRPLPVPALRYWHGRAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V67</accession>
    <name>Q97V67_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90452">
        <property type="entry name" value="G90452"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990235.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V67"/>
    <dbReference type="PaxDb" id="273057-SSO2768"/>
    <dbReference type="EnsemblBacteria" id="AAK42878">
        <property type="protein sequence ID" value="AAK42878"/>
        <property type="gene ID" value="SSO2768"/>
    </dbReference>
    <dbReference type="GeneID" id="1452797"/>
    <dbReference type="KEGG" id="sso:SSO2768"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2849"/>
    <dbReference type="eggNOG" id="arCOG08352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1375574_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V67"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="201" mass="23524" checksum="43A0979A4D07FCAA" modified="2001-10-01" version="1">MIFKYSNGTISSEGLTLCTVKVERNQIRVEGNYNFLLKREGLDSYEIYQYNSKIGEIKNFNLQYSIFNFVVSRPQLVAFKRGYENIVKIFTNSNTEVGEIKRVQDGLEGYLNDAYDPYIILIYLVVLSNFINVISYPKYRTSRVSKYRGLFYFIPLLLILVYLIPLPFYIDLAIYVALLIIFYYLLVIRRILILSPRAAHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V68</accession>
    <name>Q97V68_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90452">
        <property type="entry name" value="F90452"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V68"/>
    <dbReference type="STRING" id="273057.SSO2766"/>
    <dbReference type="PaxDb" id="273057-SSO2766"/>
    <dbReference type="EnsemblBacteria" id="AAK42877">
        <property type="protein sequence ID" value="AAK42877"/>
        <property type="gene ID" value="SSO2766"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2766"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2848"/>
    <dbReference type="eggNOG" id="arCOG07331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_604985_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V68"/>
    <dbReference type="PhylomeDB" id="Q97V68"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="191"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="259"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="452" mass="52042" checksum="CAD443478C5F0FA4" modified="2001-10-01" version="1">MMQTYFVPLAVDQNYNEINFHKQIAISLLNLDLEKKEKVVRASIIGWPLLIKKTEQGFLVLDQTLRVSSRILKYIYPPFNDVASEFSSMNDYTTFVSNLKKINLKRVSSNEITLIGLLNIEIDKLLKVAKNSVNANYQLFMLDSKLSDHDVKVIKDTLISLKAEAIFTITSLESLVKEVDDVRVRIKKGYASKLEATTKKYNELIENKKKEIDNEVQKANSEIYNETNSEISSRISRLTDITTRHIVVSLKYEGGIVGRDEFENSKNEFENLLNEFRQIKDSVAGKYLEKIKNLRKELDSLYSERNSEIENINKLMKDLDNVTNDFKNDANKVKENIENFIKYIESFYNTKLDMAEDSTLVIPFLIAKTNTGNTLVVQPQVYKGKTRGILGKVFKKSDLSEPLLNLQVFTEYLKTIDIIDNVKIHSIQINNALKEINDEGWRSLDSLEEIYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V85</accession>
    <name>Q97V85_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90450">
        <property type="entry name" value="E90450"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990261.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V85"/>
    <dbReference type="STRING" id="273057.SSO2750"/>
    <dbReference type="PaxDb" id="273057-SSO2750"/>
    <dbReference type="EnsemblBacteria" id="AAK42860">
        <property type="protein sequence ID" value="AAK42860"/>
        <property type="gene ID" value="SSO2750"/>
    </dbReference>
    <dbReference type="GeneID" id="1452779"/>
    <dbReference type="KEGG" id="sso:SSO2750"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2829"/>
    <dbReference type="eggNOG" id="arCOG03738">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_614847_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V85"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="38"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="449" mass="52234" checksum="ECE2DAEA9AD00B2F" modified="2001-10-01" version="1">MVCEIPRVTVTTWSSRSIILVGPTYRKYLEKTLEIVRNNEVASVVGQPGMGKTTLLKKIEEITKTDNLSIYLDLANKQSIDEEFWTKTTQFELREKALLELQKIKGKLGYSFWKRLMGVKFEDWLLKICNKYNNPFLRVYCLSYERNFDGMINALRDMKNLSKLVLLIDEIRDYHLSLIHRLINAGLDIPIIMAMPTEVYSKVSDLAIRRRIEESRIPLDDALTSEDIKEIVEAYCKDLTEDILPIIITLWNSKEITNVSSILQFIRNEMDKVEKVCEKNNIDCIRSELRKYTSLRNPEVDAKEFEKKVRELLSNISKEFGISYVHPRGKRIEVDGKSIVVGLFFIVDDQVYLGDVVFSNDGSLYNYEELKLLSKIEEVEHDKKVYKVRGRFVITNTATNINNIYTLNIPTLEVVKILNGDVFLLEERLKILFDQYQTPTRNETENLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VC2</accession>
    <name>Q97VC2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Extracellular solute-binding protein family 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2712</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90445">
        <property type="entry name" value="H90445"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988641.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VC2"/>
    <dbReference type="STRING" id="273057.SSO2712"/>
    <dbReference type="PaxDb" id="273057-SSO2712"/>
    <dbReference type="EnsemblBacteria" id="AAK42823">
        <property type="protein sequence ID" value="AAK42823"/>
        <property type="gene ID" value="SSO2712"/>
    </dbReference>
    <dbReference type="GeneID" id="1452742"/>
    <dbReference type="KEGG" id="sso:SSO2712"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2793"/>
    <dbReference type="eggNOG" id="arCOG00156">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_590033_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VC2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050490">
        <property type="entry name" value="Bact_solute-bd_prot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43649">
        <property type="entry name" value="ARABINOSE-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43649:SF29">
        <property type="entry name" value="OSMOPROTECTIVE COMPOUNDS-BINDING PROTEIN GGTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="53127" checksum="5F4D4490499672C5" modified="2001-10-01" version="1">MKALSTLAMAVIIIVVIAVVAAAAYLITSSSHHPSISTTTTPIIATNTTAPITLTVVTFSGQSANFIQYAGNLFHQLHPNVQVEVIQYPFSEYIQKELTALEAHSSQYDIIGFTSTSALDVASYLLPINESVFNFSDIIYPQEDFGGLYYNVSTNKTEVIGIAYETAVYLMAYNATIFNNQTLAQEFEQEYHMNFSPITYKNWSVVLDVDQFLTSHHITKYGFLIDDHVAHGIIDAFPAVFGWYYFRNNSLNMGNPAGLPNYNIMFEGRILPGFNYPLPSFNSSSGVQALITYRELVSYEPSPSQIQISYDNLPAFFSQGAGAFLFTSQLSYINNSKDVLLAPLPGGYAETGTDFLGISKYSSHPQLALEFLQFLVSPKVQEIAFLKYGKFPISKQAFLSLISNSSLPSYKREWLQETYYAALNATANPPNIPQTYPALIPSFNNEAFQFLTSPQYNETYAMNVLQQAANAWIKALSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VK0</accession>
    <name>Q97VK0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Enoyl CoA hydratase (PaaF-7)</fullName>
            <ecNumber evidence="4">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">paaF-7</name>
        <name evidence="4" type="ordered locus">SSO2624</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90436">
        <property type="entry name" value="A90436"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989350.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VK0"/>
    <dbReference type="FunCoup" id="Q97VK0">
        <property type="interactions" value="251"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2624"/>
    <dbReference type="PaxDb" id="273057-SSO2624"/>
    <dbReference type="EnsemblBacteria" id="AAK42744">
        <property type="protein sequence ID" value="AAK42744"/>
        <property type="gene ID" value="SSO2624"/>
    </dbReference>
    <dbReference type="GeneID" id="1452663"/>
    <dbReference type="KEGG" id="sso:SSO2624"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2706"/>
    <dbReference type="eggNOG" id="arCOG00239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_2"/>
    <dbReference type="InParanoid" id="Q97VK0"/>
    <dbReference type="PhylomeDB" id="Q97VK0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000009">
        <property type="entry name" value="Carnitinyl-CoA dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="266" mass="29753" checksum="C331801304B6DB06" modified="2001-10-01" version="1">MQVNYIYMYSTIQIEVIDNIGIIKLNRPDKLNAINFQMVDELVDVLNKLDNDDKIKVVIITGNGKAFSAGADVKEMLETPLEEIMKKGHMPLWEKLRTFKKPVIAALNGITAGGGLELAMACDIIIASESAKLGQPEINLGIMPGAGGTQRLTRVLGKYKAMELVLTGKLIDSKEAERYGLVNKVVPDNSLIDEAIRLAREIAEKPIISIILAKEAVARAWDTLLQQGLDFERRNFYLALNTKEAKEGMRAFLEKRKPRWENDKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VK1</accession>
    <name>Q97VK1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enoyl CoA hydratase (PaaF-6)</fullName>
            <ecNumber evidence="2">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">paaF-6</name>
        <name evidence="2" type="ordered locus">SSO2623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.17" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90435">
        <property type="entry name" value="H90435"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989349.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VK1"/>
    <dbReference type="FunCoup" id="Q97VK1">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2623"/>
    <dbReference type="PaxDb" id="273057-SSO2623"/>
    <dbReference type="EnsemblBacteria" id="AAK42743">
        <property type="protein sequence ID" value="AAK42743"/>
        <property type="gene ID" value="SSO2623"/>
    </dbReference>
    <dbReference type="GeneID" id="1452662"/>
    <dbReference type="KEGG" id="sso:SSO2623"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2705"/>
    <dbReference type="eggNOG" id="arCOG00239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_2_2"/>
    <dbReference type="InParanoid" id="Q97VK1"/>
    <dbReference type="PhylomeDB" id="Q97VK1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004725">
        <property type="entry name" value="PRK06072.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43459:SF1">
        <property type="entry name" value="EG:BACN32G11.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43459">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="249" mass="28058" checksum="AEE17188E656700E" modified="2001-10-01" version="1">MIKVDNKDGYVVVTISRADKLNALNLEMRNELISKLKEINANPKIRTVILTGEGRAFCVGADVNEFAPDFTIDLRETFYPIIREIRFSDKIYIAAINGVTAGACIGISLSTDFKFAKREVKFVTAFQRLGLASDTGVAYFLLKLTRDQRAYEIAVLGGEFTAEDAERWGLLKISENPLYDAEEMANKILNGPFQSYIAGKRMANLVLYNDLERFLEYESAIQGYLGKTEDFKEGISSFKEKREPKFKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VM3</accession>
    <name>Q97VM3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-L-isoaspartate(D-aspartate) O-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.77</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pcm</name>
        <name evidence="10" type="ordered locus">SSO2599</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12143"/>
            <dbReference type="Rhea" id="RHEA-COMP:12144"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90596"/>
            <dbReference type="ChEBI" id="CHEBI:90598"/>
            <dbReference type="EC" id="2.1.1.77"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90433">
        <property type="entry name" value="B90433"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989313.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VM3"/>
    <dbReference type="FunCoup" id="Q97VM3">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2599"/>
    <dbReference type="PaxDb" id="273057-SSO2599"/>
    <dbReference type="EnsemblBacteria" id="AAK42721">
        <property type="protein sequence ID" value="AAK42721"/>
        <property type="gene ID" value="SSO2599"/>
    </dbReference>
    <dbReference type="GeneID" id="1454048"/>
    <dbReference type="KEGG" id="sso:SSO2599"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2680"/>
    <dbReference type="eggNOG" id="arCOG00976">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055432_2_0_2"/>
    <dbReference type="InParanoid" id="Q97VM3"/>
    <dbReference type="PhylomeDB" id="Q97VM3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF0">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE(D-ASPARTATE) O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="236" mass="26972" checksum="8DF08EF5E20F83FF" modified="2001-10-01" version="1">MSAKDDILRSIKNSKLANAFIKVNREDFLPQLLKKYAYDPNYVDKPFYITPNVTTTALSLGMYMLDILNLGETQKVLEIGTGIGYYTALMAEVVGDNNVISLEIDDTIFEYAKNILLPKYPRIKLIKTDGSLGYDKEAPYDRIIIWAAAPTVPCKLYDQLRENGIMVVPIGSEKAQGLYRITKIGYEPKIERLGDVIFMKMRGLFGFYEDDDPNERRIKKIEEKVNRLLSKFMSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VM7</accession>
    <name>Q97VM7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2594</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90432">
        <property type="entry name" value="F90432"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VM7"/>
    <dbReference type="PaxDb" id="273057-SSO2594"/>
    <dbReference type="EnsemblBacteria" id="AAK42717">
        <property type="protein sequence ID" value="AAK42717"/>
        <property type="gene ID" value="SSO2594"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2594"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2675"/>
    <dbReference type="eggNOG" id="arCOG07174">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2191139_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VM7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="126" mass="14258" checksum="D12AA6C216524333" modified="2001-10-01" version="1">MKGKVINIESDITSWLRIMIGCKDTSCVKDTLNALLNRYGIGKNITEIVLENIDGLATYKDNKVIINVLKYDEIANEASGQSEIVSAFLLLSSLYSLVGTKRMEEIIRNEYGKESPIYKLYEILFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VQ7</accession>
    <name>Q97VQ7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2556</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90428">
        <property type="entry name" value="F90428"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VQ7"/>
    <dbReference type="STRING" id="273057.SSO2556"/>
    <dbReference type="PaxDb" id="273057-SSO2556"/>
    <dbReference type="EnsemblBacteria" id="AAK42685">
        <property type="protein sequence ID" value="AAK42685"/>
        <property type="gene ID" value="SSO2556"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2556"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2638"/>
    <dbReference type="eggNOG" id="arCOG08310">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2021541_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VQ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="125" mass="14330" checksum="E85B062D14946EBA" modified="2001-10-01" version="1">MTMEPLIAIDLNSNMSISQLESSVKKLFETFGALDVVFIIDDDSIVELDGNLVLTFYSVKELVETYKVLKKLSEVKSNRLRVTSVIRLERDLKRFPLVVITDRKIVGLNKNLIFVYDGDKVKARY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VR3</accession>
    <name>Q97VR3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90427">
        <property type="entry name" value="G90427"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923918.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VR3"/>
    <dbReference type="PaxDb" id="273057-SSO2550"/>
    <dbReference type="EnsemblBacteria" id="AAK42678">
        <property type="protein sequence ID" value="AAK42678"/>
        <property type="gene ID" value="SSO2550"/>
    </dbReference>
    <dbReference type="GeneID" id="15299136"/>
    <dbReference type="KEGG" id="sso:SSO2550"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2630"/>
    <dbReference type="eggNOG" id="arCOG08532">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2340316_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VR3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="100" mass="12115" checksum="64589AC4940DAECB" modified="2001-10-01" version="1">MKTMTMAEALNKIMKNRTTTLKLSYYPFLLAVKDNVPELDIMLKRKRELNYYLDKLYSESKKYLLKYAKDKYYCVCGRTFPSAYDFYTHVFREHKLSIRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VS4</accession>
    <name>Q97VS4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glucan phosphorylase, putative (GlgP or malP)</fullName>
            <ecNumber evidence="2">2.4.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">glgP or malP</name>
        <name evidence="2" type="ordered locus">SSO2538</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.1" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90426">
        <property type="entry name" value="D90426"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990987.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VS4"/>
    <dbReference type="FunCoup" id="Q97VS4">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2538"/>
    <dbReference type="CAZy" id="GT35">
        <property type="family name" value="Glycosyltransferase Family 35"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2538"/>
    <dbReference type="EnsemblBacteria" id="AAK42667">
        <property type="protein sequence ID" value="AAK42667"/>
        <property type="gene ID" value="SSO2538"/>
    </dbReference>
    <dbReference type="GeneID" id="1453994"/>
    <dbReference type="KEGG" id="sso:SSO2538"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2614"/>
    <dbReference type="eggNOG" id="arCOG01421">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_552792_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VS4"/>
    <dbReference type="PhylomeDB" id="Q97VS4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004645">
        <property type="term" value="F:1,4-alpha-oligoglucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052182">
        <property type="entry name" value="Glycogen/Maltodextrin_Phosph"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42655">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42655:SF1">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="264"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="465" mass="53630" checksum="7BA2501B832164CF" modified="2001-10-01" version="1">MIVSVTAEIGLDIGQNFAGGLGILEGDKFYAAARLGINYTVITLLYRRGYADGEGKQRELLSHLNKEWENEITVGDQRIMVEYLAYTLNTAKVIFANPLNTNLNDQLYVENSNDERFYKCLLLAKVAENYISERIGWDKVKFVDMQEACPAFLPLLRYFPRYRIVVHTPAPWGHPTFPSHLFKKEFSFEFPLDHVVMTDIGLSASIEGIVVSKKMLKHVSRTFPQHMHKIRAVTNGIEISRWRNPSLNMVKDLDDFMKKRIEVKRDSIKKLGKDTDKPTIAWLRRITGYKRPDFILRLIDDLKDDVFFIIGGLAHPKDYSAVEIERKFKEISDKRNNVIYIRGADANFMKLAIWASDIWTFTPYSGWEASGTSFMKAGINGIPSVASRDGAVTEIINDGYNGWLYGEDRDILLPFNVYDDNKEYEEFINKIKVALNKYYEVGYNAYKTFPAFCSIDRLMKEYGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W35</accession>
    <name>Q97W35_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase</fullName>
            <shortName evidence="5">HPPK</shortName>
            <ecNumber evidence="5">2.7.6.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">6-hydroxymethyl-7,8-dihydropterin diphosphokinase</fullName>
            <shortName evidence="5">6-HMPDK</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">7,8-dihydro-6-hydroxymethylpterin diphosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase</fullName>
            <shortName evidence="5">PPPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mptE</name>
        <name evidence="7" type="ordered locus">SSO2408</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of diphosphate from ATP to 6-hydroxymethyl-7,8-dihydropterin (6-HMD), leading to 6-hydroxymethyl-7,8-dihydropterin diphosphate (6-HMDP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal 6-HMPDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.3" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90412">
        <property type="entry name" value="D90412"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W35"/>
    <dbReference type="STRING" id="273057.SSO2408"/>
    <dbReference type="PaxDb" id="273057-SSO2408"/>
    <dbReference type="EnsemblBacteria" id="AAK42555">
        <property type="protein sequence ID" value="AAK42555"/>
        <property type="gene ID" value="SSO2408"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2408"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2490"/>
    <dbReference type="eggNOG" id="arCOG04303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093043_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W35"/>
    <dbReference type="PhylomeDB" id="Q97W35"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02131">
        <property type="entry name" value="HMPDK_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027510">
        <property type="entry name" value="HMPDK_MptE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002826">
        <property type="entry name" value="MptE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648">
        <property type="entry name" value="6-HYDROXYMETHYL-7,8-DIHYDROPTERIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648:SF1">
        <property type="entry name" value="6-HYDROXYMETHYL-7,8-DIHYDROPTERIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01973">
        <property type="entry name" value="MptE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="6-hydroxymethylpterin diphosphokinase MptE-like" evidence="6">
        <location>
            <begin position="31"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="204" mass="23145" checksum="2C714E6600153EC1" modified="2001-10-01" version="1">MIRSWFGFKERDDYISASILNYLITKEYDEAELKELIKGREIAIVGAGPQLDKINKLKEDVIIAADGAANYLVDIGIVPDIIVTDLDGLQTFPKNPIYVVLAHGDNINLLHKVKEMDKVIPNSQVMPFGRLRLYGGFTDGDRAVVLAKYMKASKIRLYAMDFQSGIVGKFSKPYYQRNVPASMIKRKKLEIARMIIEQVLNYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W64</accession>
    <name>Q97W64_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2373</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90408">
        <property type="entry name" value="E90408"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W64"/>
    <dbReference type="SMR" id="Q97W64"/>
    <dbReference type="FunCoup" id="Q97W64">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2373"/>
    <dbReference type="PaxDb" id="273057-SSO2373"/>
    <dbReference type="EnsemblBacteria" id="AAK42524">
        <property type="protein sequence ID" value="AAK42524"/>
        <property type="gene ID" value="SSO2373"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2373"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2458"/>
    <dbReference type="eggNOG" id="arCOG04150">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064992_3_1_2"/>
    <dbReference type="InParanoid" id="Q97W64"/>
    <dbReference type="PhylomeDB" id="Q97W64"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000076">
        <property type="entry name" value="KH domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019964">
        <property type="entry name" value="KH_domain_protein_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055211">
        <property type="entry name" value="KH_PNO1_2nd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03665">
        <property type="entry name" value="arCOG04150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010326">
        <property type="entry name" value="PRK13763.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826">
        <property type="entry name" value="RIBONUCLEASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="RNA-BINDING PROTEIN PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22891">
        <property type="entry name" value="KH_PNO1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="2">
        <location>
            <begin position="78"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="189" mass="21483" checksum="F9CC2CF2BA636833" modified="2001-10-01" version="1">MIILFITVEDEKLEVVKKVIGKLEQFTDTKIIYDERTKTFNILPKGQNQYETLKAVSVIRAIGLGFDEQSAFRLLSDEYTLDVIDLKALIGSNPDTIRRVKGRIIGENGKTKRIIQEYTGVDISIYGHYIGVLGPYDQVQIAKKAIELLIDGKEHSSVYKFLDKAEKDLIIYKTSKLRRKGISEIKDNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W68</accession>
    <name>Q97W68_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2369</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90408">
        <property type="entry name" value="A90408"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989474.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W68"/>
    <dbReference type="STRING" id="273057.SSO2369"/>
    <dbReference type="PaxDb" id="273057-SSO2369"/>
    <dbReference type="EnsemblBacteria" id="AAK42520">
        <property type="protein sequence ID" value="AAK42520"/>
        <property type="gene ID" value="SSO2369"/>
    </dbReference>
    <dbReference type="GeneID" id="1453847"/>
    <dbReference type="KEGG" id="sso:SSO2369"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2454"/>
    <dbReference type="eggNOG" id="arCOG05956">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170079_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W68"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="104" mass="12157" checksum="E722FD25B9F9D9A0" modified="2001-10-01" version="1">MDSGITIIAEKLIDPSVKKACKMMVKDDEIIKLVGISSKKLALNVIDKVSFWLVYENNNLLYCKLCNRGPFTKKGLYLHLSRVHRREIREMLEEELRHEIRTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WA8</accession>
    <name>Q97WA8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH marR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2327</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99403">
        <property type="entry name" value="A99403"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923833.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WA8"/>
    <dbReference type="STRING" id="273057.SSO2327"/>
    <dbReference type="PaxDb" id="273057-SSO2327"/>
    <dbReference type="EnsemblBacteria" id="AAK42480">
        <property type="protein sequence ID" value="AAK42480"/>
        <property type="gene ID" value="SSO2327"/>
    </dbReference>
    <dbReference type="GeneID" id="1453807"/>
    <dbReference type="KEGG" id="sso:SSO2327"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2413"/>
    <dbReference type="eggNOG" id="arCOG05969">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1032970_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WA8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053515">
        <property type="entry name" value="HTH-type_ArnR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041016">
        <property type="entry name" value="trans_reg_ArnR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF4">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13463">
        <property type="entry name" value="HTH_27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="HTH marR-type" evidence="2">
        <location>
            <begin position="23"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="270" mass="30955" checksum="4650C04E5F529912" modified="2001-10-01" version="1">MSYNVFDVLRELDSIVDFARAKLQWDILFFINSRGPSSISEIAEGTNNSKKAVIDAIRKLVEKELIIKVKYDVYDLSEKGKQVLNKLNYFTSHTVSTQKSVDGDNNVLSNNADNPSQNYYLLELIKMSLLNNGTLPIDKVSRELGISKQTVKYYLELFMRKKIFKKVNKKSLLGKSVQTVVLTSEGRKIAYKVPSLIKIRNNLFLRLLLKITFSISYESALMKLMVFFALSSPIIIYYDGDPPVRILGIVWLYMLVFTSLLSIFAYLTMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WH2</accession>
    <name>Q97WH2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Probable cation transport protein (CzcD)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">czcD</name>
        <name evidence="11" type="ordered locus">SSO2247</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90394">
        <property type="entry name" value="H90394"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923814.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WH2"/>
    <dbReference type="FunCoup" id="Q97WH2">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2247"/>
    <dbReference type="PaxDb" id="273057-SSO2247"/>
    <dbReference type="EnsemblBacteria" id="AAK42415">
        <property type="protein sequence ID" value="AAK42415"/>
        <property type="gene ID" value="SSO2247"/>
    </dbReference>
    <dbReference type="GeneID" id="1453742"/>
    <dbReference type="KEGG" id="sso:SSO2247"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2342"/>
    <dbReference type="eggNOG" id="arCOG01477">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_0_4_2"/>
    <dbReference type="InParanoid" id="Q97WH2"/>
    <dbReference type="PhylomeDB" id="Q97WH2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071577">
        <property type="term" value="P:zinc ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="entry name" value="Cation efflux protein, cytoplasmic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050681">
        <property type="entry name" value="CDF/SLC30A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562">
        <property type="entry name" value="CATION EFFLUX PROTEIN/ ZINC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562:SF17">
        <property type="entry name" value="RE54080P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="9">
        <location>
            <begin position="4"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="10">
        <location>
            <begin position="210"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK42415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="271" mass="30277" checksum="D9FF1514758F6904" modified="2001-10-01" version="1">MLRTFVLMFFTSLSFIVAYYITFSPLILAEFSHALIDFLTIAFSIIAIKFIGENEVAEGRFSYGLHRLEVVVAMINLIIIILLSLAVAYTSVTSLFKETTNDSFTLIVSSALASFLTYFASPKERNDLGKKGLYMHILSDFLGYIMGFIIGILIFISGIRELDPVGAIILVILNFALSIPLLKEAFLIFMEGSSVKIDHIMEELTKISPSVHHLHVWSICDHVKVATLHVKVSPNLTIAEADKIRGSICDFLKEKYGISHVTVQFETNVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WP3</accession>
    <name>Q97WP3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2167</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90385">
        <property type="entry name" value="H90385"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992139.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WP3"/>
    <dbReference type="SMR" id="Q97WP3"/>
    <dbReference type="FunCoup" id="Q97WP3">
        <property type="interactions" value="160"/>
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    <dbReference type="EnsemblBacteria" id="AAK42343">
        <property type="protein sequence ID" value="AAK42343"/>
        <property type="gene ID" value="SSO2167"/>
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    <dbReference type="PATRIC" id="fig|273057.12.peg.2261"/>
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    <dbReference type="HOGENOM" id="CLU_062658_5_1_2"/>
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        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="CDD" id="cd03424">
        <property type="entry name" value="NUDIX_ADPRase_Nudt5_UGPPase_Nudt14"/>
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        <property type="entry name" value="NUDIX hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
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        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
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        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
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        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839:SF18">
        <property type="entry name" value="NUDIX HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
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        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="29"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="166" mass="19341" checksum="91218A984B3CD2ED" modified="2001-10-01" version="1">MKIFSGKKFEVHIDKVKLPNGYERELEFVKHRGSVVIIPKINNEIILIRQFRPVIDKWIYELPAGTIEEGEDPLNTANRELIEEIGYEAGKMKEIISFYASPGITTEYMRLYLAEDLRYVGAKPEPYEIIEPIRLSIEEAIKMIRERKIEDAKTIIGIFTLKELLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WQ1</accession>
    <name>Q97WQ1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Indolepyruvate ferredoxin oxidoreductase beta subunit (IorB)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">iorB</name>
        <name evidence="3" type="ordered locus">SSO2069</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90374">
        <property type="entry name" value="D90374"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990192.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WQ1"/>
    <dbReference type="STRING" id="273057.SSO2069"/>
    <dbReference type="PaxDb" id="273057-SSO2069"/>
    <dbReference type="EnsemblBacteria" id="AAK42251">
        <property type="protein sequence ID" value="AAK42251"/>
        <property type="gene ID" value="SSO2069"/>
    </dbReference>
    <dbReference type="GeneID" id="1453578"/>
    <dbReference type="KEGG" id="sso:SSO2069"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2146"/>
    <dbReference type="eggNOG" id="arCOG01602">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087284_1_0_2"/>
    <dbReference type="InParanoid" id="Q97WQ1"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IorB_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
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        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
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        <property type="entry name" value="PRK06853.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43854">
        <property type="entry name" value="INDOLEPYRUVATE OXIDOREDUCTASE SUBUNIT IORB"/>
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    </dbReference>
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        <property type="entry name" value="INDOLEPYRUVATE OXIDOREDUCTASE SUBUNIT IORB"/>
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    </dbReference>
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        <property type="entry name" value="POR"/>
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    </dbReference>
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        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="12"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="194" mass="20781" checksum="A18D1AFBFAEF03FF" modified="2001-10-01" version="1">MAWVNILIAGVGGQGIITAGKIIAEAGNYSNTKVLIAETHGLAQRGGGVNVHVRIGDVNSPLIPLGKADYLVGLEAIEVLRNLNYASRKHTTIVVNNYVVRPVLPKVKLLTLQEIFDKLKGCKVYVIDANQIAIKAGNIKAANAAMLGFLYSLGAFEGLINEESFIKALKYESNIKAFKIAQAIKINGIDINGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WQ4</accession>
    <name>Q97WQ4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2064</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2010" name="Acta Crystallogr. F" volume="66" first="1160" last="1166">
            <title>The structure of SSO2064, the first representative of Pfam family PF01796, reveals a novel two-domain zinc-ribbon OB-fold architecture with a potential acyl-CoA-binding role.</title>
            <authorList>
                <person name="Krishna S.S."/>
                <person name="Aravind L."/>
                <person name="Bakolitsa C."/>
                <person name="Caruthers J."/>
                <person name="Carlton D."/>
                <person name="Miller M.D."/>
                <person name="Abdubek P."/>
                <person name="Astakhova T."/>
                <person name="Axelrod H.L."/>
                <person name="Chiu H.J."/>
                <person name="Clayton T."/>
                <person name="Deller M.C."/>
                <person name="Duan L."/>
                <person name="Feuerhelm J."/>
                <person name="Grant J.C."/>
                <person name="Han G.W."/>
                <person name="Jaroszewski L."/>
                <person name="Jin K.K."/>
                <person name="Klock H.E."/>
                <person name="Knuth M.W."/>
                <person name="Kumar A."/>
                <person name="Marciano D."/>
                <person name="McMullan D."/>
                <person name="Morse A.T."/>
                <person name="Nigoghossian E."/>
                <person name="Okach L."/>
                <person name="Reyes R."/>
                <person name="Rife C.L."/>
                <person name="van den Bedem H."/>
                <person name="Weekes D."/>
                <person name="Xu Q."/>
                <person name="Hodgson K.O."/>
                <person name="Wooley J."/>
                <person name="Elsliger M.A."/>
                <person name="Deacon A.M."/>
                <person name="Godzik A."/>
                <person name="Lesley S.A."/>
                <person name="Wilson I.A."/>
            </authorList>
            <dbReference type="PubMed" id="20944206"/>
            <dbReference type="DOI" id="10.1107/s1744309110002514"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH ZN(2+)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99374">
        <property type="entry name" value="A99374"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988836.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3IRB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B=1-144"/>
    </dbReference>
    <dbReference type="PDBsum" id="3IRB"/>
    <dbReference type="AlphaFoldDB" id="Q97WQ4"/>
    <dbReference type="SMR" id="Q97WQ4"/>
    <dbReference type="STRING" id="273057.SSO2064"/>
    <dbReference type="PaxDb" id="273057-SSO2064"/>
    <dbReference type="EnsemblBacteria" id="AAK42248">
        <property type="protein sequence ID" value="AAK42248"/>
        <property type="gene ID" value="SSO2064"/>
    </dbReference>
    <dbReference type="GeneID" id="1453575"/>
    <dbReference type="KEGG" id="sso:SSO2064"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2143"/>
    <dbReference type="eggNOG" id="arCOG01288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119412_2_0_2"/>
    <dbReference type="InParanoid" id="Q97WQ4"/>
    <dbReference type="PhylomeDB" id="Q97WQ4"/>
    <dbReference type="EvolutionaryTrace" id="Q97WQ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="ChsH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="ChsH2_Znr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF4">
        <property type="entry name" value="DUF35 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_ChsH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="5" id="KW-0002">3D-structure</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="2">
        <location>
            <begin position="36"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="ChsH2 C-terminal OB-fold" evidence="1">
        <location>
            <begin position="74"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="3IRB"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16467" checksum="EAA5BF52DCBD44F8" modified="2001-10-01" version="1">MSWEKSGKEGSLLRWYDVMEAERYEYTVGPAGEQFFNGLKQNKIIGSKCSKCGRIFVPARSYCEHCFVKIENYVEINKDEAYVDSYTIIYNDDEGNKLAQPVYIALIRFPNIEGGLLCYAEGNVKVGAKAKILSFQWPLRVKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WS2</accession>
    <name>Q97WS2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gdhA-4</name>
        <name evidence="10" type="ordered locus">SSO2044</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90371">
        <property type="entry name" value="G90371"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WS2"/>
    <dbReference type="SMR" id="Q97WS2"/>
    <dbReference type="FunCoup" id="Q97WS2">
        <property type="interactions" value="250"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2044"/>
    <dbReference type="PaxDb" id="273057-SSO2044"/>
    <dbReference type="EnsemblBacteria" id="AAK42230">
        <property type="protein sequence ID" value="AAK42230"/>
        <property type="gene ID" value="SSO2044"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2044"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2125"/>
    <dbReference type="eggNOG" id="arCOG01352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_1_2_2"/>
    <dbReference type="InParanoid" id="Q97WS2"/>
    <dbReference type="PhylomeDB" id="Q97WS2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000003">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="187"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="7">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="420" mass="46063" checksum="C500B0A5C9045842" modified="2001-10-01" version="1">MMEEVLSSSLYTQQVKKLYKVGELLGLDNETLETLSQPERIIQVKIQIRGSDGKLKTFMGWRSQHNSALGPYKGGIRYHPNVTQDEVEALSMIMTWKNSLLLLPYGGGKGGIRVDPKKLTKEELEQLSRKFIQAIYKYLGSELDIPAPDVNTDSQTMAWYLDEYIKITGNVDFAVFTGKPVELGGIGVRLYSTGLGVATIAKEAANKFIGGIEEARVIIQGFGNVGYYAAKFLSDMGAKIVGISDSKGGVINENGIDVGKAMEIKEKTGSVTNYPEGRKVTNEELLISDCNILVPAALENVINKFNAPKIKAKLIVEGANGPLTADADEIMRQRGIVVVPDILANAGGVVGSYVEWANNKMGQIISDEEAKKLIVDRMNNAFNTLYDYHQKKLEDHDLRTAAMALAVDRVVRAMKARGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WT8</accession>
    <name>Q97WT8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1286 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90369">
        <property type="entry name" value="F90369"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923734.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WT8"/>
    <dbReference type="STRING" id="273057.SSO2026"/>
    <dbReference type="PaxDb" id="273057-SSO2026"/>
    <dbReference type="EnsemblBacteria" id="AAK42213">
        <property type="protein sequence ID" value="AAK42213"/>
        <property type="gene ID" value="SSO2026"/>
    </dbReference>
    <dbReference type="GeneID" id="1453540"/>
    <dbReference type="KEGG" id="sso:SSO2026"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2106"/>
    <dbReference type="eggNOG" id="arCOG05320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117041_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WT8"/>
    <dbReference type="PhylomeDB" id="Q97WT8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009705">
        <property type="entry name" value="DUF1286"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06939">
        <property type="entry name" value="DUF1286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="20259" checksum="F916F8BA364D39D5" modified="2001-10-01" version="1">MNLVRLRTHYIFSTGLLTLLDSVLFHEYFYYALILSGIVSVIGNSLIDRIGHKEIATRYGYIPVRTPLTHTIPRSVVWGIVSVVPVFILLLIYYYGFSYHEYYFSLSNKVVLLILLNGVVVGPSHLFLDVFTERGIYVKKYGRWRRFALAHFRYDNPLVNGLAIIAGAVMIYLAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-10-02" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WV6</accession>
    <name>Q97WV6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1048</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90367">
        <property type="entry name" value="C90367"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WV6"/>
    <dbReference type="STRING" id="273057.SSO2006"/>
    <dbReference type="PaxDb" id="273057-SSO2006"/>
    <dbReference type="EnsemblBacteria" id="AAK42194">
        <property type="protein sequence ID" value="AAK42194"/>
        <property type="gene ID" value="SSO2006"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2006"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2081"/>
    <dbReference type="eggNOG" id="arCOG02127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124929_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WV6"/>
    <dbReference type="PhylomeDB" id="Q97WV6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13565">
        <property type="entry name" value="HTH_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="157" mass="18570" checksum="B7E05FED8E9BEFC3" modified="2001-10-01" version="1">MVQAYEEEKDARIKERILSVKLHLVDGKSEKKVSKMLNKGYSTIKLWVGKYKKEGLNGLRDKPRSGRPRKAEKGKIKQILEDQPQKYGIQEEYWTMRTFKIALQEQGIEYKKSRLYELVHELGYNLVKPRPTNVQAKKDMWDDFKIGRRGSLLSRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WW5</accession>
    <name>Q97WW5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO9455</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK67524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WW5"/>
    <dbReference type="PaxDb" id="273057-SSO9455"/>
    <dbReference type="EnsemblBacteria" id="AAK67524">
        <property type="protein sequence ID" value="AAK67524"/>
        <property type="gene ID" value="SSO9455"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO9455"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208840_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WW5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK67524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="41" mass="5020" checksum="4041B85944081C7C" modified="2001-10-01" version="1">MGREEFRLLLILLFPDLFNPENFTFNLVDTLIYTIDLFLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X26</accession>
    <name>Q97X26_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1926</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90358">
        <property type="entry name" value="C90358"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923656.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X26"/>
    <dbReference type="STRING" id="273057.SSO1926"/>
    <dbReference type="PaxDb" id="273057-SSO1926"/>
    <dbReference type="EnsemblBacteria" id="AAK42122">
        <property type="protein sequence ID" value="AAK42122"/>
        <property type="gene ID" value="SSO1926"/>
    </dbReference>
    <dbReference type="GeneID" id="1453449"/>
    <dbReference type="KEGG" id="sso:SSO1926"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2002"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066381_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X26"/>
    <dbReference type="PhylomeDB" id="Q97X26"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="1">
        <location>
            <begin position="6"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="2">
        <location>
            <begin position="195"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="317" mass="35936" checksum="0B9A76149B9231B1" modified="2001-10-01" version="1">MEAPVAGIDVSKDKLIVYFQGKLYEFTNDRQGFEEIRKILPSGCKVGIESTGVYHVNLAKYLNNEYDVRIINPFILKKFKGFRGKKSDKNDAKKLAEIVVSMGSGFVTSDARELTSQWDFVIRSIARVKNRLRRDLVLLGYRDSLSKKNLEEVLRGGGDSVVLSEVRFLLEELERLEGRKREIEKELEDFVPKDSLIFTIPGIGKTLGCIILARVGDVRRFSDKKRFVAYCGLDPVVESSGKSVVSRGISKRGDAVLRRAFYLAALTAIKVNLIIKRFYEEHKGRLRGKKLIVACARKLAVITWAVLYYNKPFDASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X97</accession>
    <name>Q97X97_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamine amidotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1857</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90348">
        <property type="entry name" value="F90348"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988526.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X97"/>
    <dbReference type="FunCoup" id="Q97X97">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1857"/>
    <dbReference type="PaxDb" id="273057-SSO1857"/>
    <dbReference type="EnsemblBacteria" id="AAK42045">
        <property type="protein sequence ID" value="AAK42045"/>
        <property type="gene ID" value="SSO1857"/>
    </dbReference>
    <dbReference type="GeneID" id="1453372"/>
    <dbReference type="KEGG" id="sso:SSO1857"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1907"/>
    <dbReference type="eggNOG" id="arCOG00090">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054974_3_1_2"/>
    <dbReference type="InParanoid" id="Q97X97"/>
    <dbReference type="PhylomeDB" id="Q97X97"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01741">
        <property type="entry name" value="GATase1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000033">
        <property type="entry name" value="Glutamine amidotransferase class-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695:SF5">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="1">
        <location>
            <begin position="28"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="209" mass="23890" checksum="C309F61D85B84F31" modified="2001-10-01" version="1">MKILAVYNHPVETLGTLKKFLPRVNEIMAEELKGDEQFEALIIMGGPMGVYERDKYHFLKVEIDLIKKGYKENKRILGVCLGSQLIAEALGGKVIKGGFGQEIGIQKVKLLGELKDFMREDELSVFQWHGDTFSLPQDAVLLAYSNNYFQAFKVKRALGVQFHLEVNSEMVSQWIKLYGGDESVANYVKRFEGELEDAAFKLIQYWMQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XL3</accession>
    <name>Q97XL3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1714</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90332">
        <property type="entry name" value="G90332"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XL3"/>
    <dbReference type="PaxDb" id="273057-SSO1714"/>
    <dbReference type="EnsemblBacteria" id="AAK41918">
        <property type="protein sequence ID" value="AAK41918"/>
        <property type="gene ID" value="SSO1714"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1714"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1762"/>
    <dbReference type="eggNOG" id="arCOG07238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1665580_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XL3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="158" mass="17717" checksum="77C1E8C0C6F02635" modified="2001-10-01" version="1">MLFSSFLLICFSLVILGFTRSLVLREFPVGDATYGLVSKILQTYSFLIYPFSFLCRSRYPSIGNHISILFCGSVFLKAKEIRKPLAPLETCIPICSKFLYALITSPPYTLSNTFSLFLPLCTSSRLYPCLLTCINALAPLPYLLMSSLSSTPDYSNYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XN2</accession>
    <name>Q97XN2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Partial transposase in ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1682</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90329">
        <property type="entry name" value="D90329"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XN2"/>
    <dbReference type="SMR" id="Q97XN2"/>
    <dbReference type="STRING" id="273057.SSO1682"/>
    <dbReference type="PaxDb" id="273057-SSO1682"/>
    <dbReference type="DNASU" id="1454670"/>
    <dbReference type="EnsemblBacteria" id="AAK41891">
        <property type="protein sequence ID" value="AAK41891"/>
        <property type="gene ID" value="SSO1682"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1682"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149102_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XN2"/>
    <dbReference type="PhylomeDB" id="Q97XN2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="50"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="123" mass="14616" checksum="092BE7CD5DDCCA19" modified="2001-10-01" version="1">MKNLRRDIYMKLGKYFAEHYDTVVMEDINVKKLVGKSLKVMRRRLHDIGFYEFRTILEYQLIKYGKKFTLVDPAYTSMTCARCGYVREDLTGVFSCSHLIRYHIVKLYQVLSNILYSLQVSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XN4</accession>
    <name>Q97XN4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90329">
        <property type="entry name" value="B90329"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992612.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XN4"/>
    <dbReference type="PaxDb" id="273057-SSO1680"/>
    <dbReference type="EnsemblBacteria" id="AAK41889">
        <property type="protein sequence ID" value="AAK41889"/>
        <property type="gene ID" value="SSO1680"/>
    </dbReference>
    <dbReference type="GeneID" id="1454668"/>
    <dbReference type="KEGG" id="sso:SSO1680"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1728"/>
    <dbReference type="eggNOG" id="arCOG03169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165245_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XN4"/>
    <dbReference type="PhylomeDB" id="Q97XN4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301:SF8">
        <property type="entry name" value="ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301">
        <property type="entry name" value="DNA-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11486" checksum="4E24C42FF284ADC0" modified="2001-10-01" version="1">MLFSVEPKESLKDLFNREEEVKKFLQCVNNERMIIVTGLRRVGKSSLVLAGLNSLNRGYILVDLRKIFDNVSKRITPDKLYEEIHFNLTKINKIYLDGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XP3</accession>
    <name>Q97XP3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-methylhydantoinase A related protein (HuyA-like)</fullName>
            <ecNumber evidence="3">3.5.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">huyA-like</name>
        <name evidence="3" type="ordered locus">SSO1668</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.-" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90328">
        <property type="entry name" value="A90328"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XP3"/>
    <dbReference type="STRING" id="273057.SSO1668"/>
    <dbReference type="PaxDb" id="273057-SSO1668"/>
    <dbReference type="DNASU" id="1454659"/>
    <dbReference type="EnsemblBacteria" id="AAK41880">
        <property type="protein sequence ID" value="AAK41880"/>
        <property type="gene ID" value="SSO1668"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1668"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1717"/>
    <dbReference type="eggNOG" id="arCOG01511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007154_1_1_2"/>
    <dbReference type="InParanoid" id="Q97XP3"/>
    <dbReference type="PhylomeDB" id="Q97XP3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF10">
        <property type="entry name" value="HYDANTOINASE_OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="2">
        <location>
            <begin position="20"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="1">
        <location>
            <begin position="207"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="529" mass="57621" checksum="66BA5769157EB0CD" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XR5</accession>
    <name>Q97XR5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIP18 family-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90325">
        <property type="entry name" value="C90325"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990902.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XR5"/>
    <dbReference type="SMR" id="Q97XR5"/>
    <dbReference type="STRING" id="273057.SSO1645"/>
    <dbReference type="PaxDb" id="273057-SSO1645"/>
    <dbReference type="DNASU" id="1454637"/>
    <dbReference type="EnsemblBacteria" id="AAK41858">
        <property type="protein sequence ID" value="AAK41858"/>
        <property type="gene ID" value="SSO1645"/>
    </dbReference>
    <dbReference type="GeneID" id="1454637"/>
    <dbReference type="KEGG" id="sso:SSO1645"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1693"/>
    <dbReference type="eggNOG" id="arCOG01852">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1998836_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XR5"/>
    <dbReference type="PhylomeDB" id="Q97XR5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="138" mass="16227" checksum="38E620A18E5AD1E3" modified="2001-10-01" version="1">MLEDVIDPETNYSIVKMGFIRNIEIEEGKIKVTLSPPTFWCPPLFLYMILEDVKRKLSESYNGVLIQVVDHHDAEKLTSCINNGKSFEECYKNEVEGNSYEAIRERFRVKRERDDRLSKLTLSINGEFCRLIYEAKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XR9</accession>
    <name>Q97XR9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1639</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90324">
        <property type="entry name" value="F90324"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XR9"/>
    <dbReference type="PaxDb" id="273057-SSO1639"/>
    <dbReference type="EnsemblBacteria" id="AAK41853">
        <property type="protein sequence ID" value="AAK41853"/>
        <property type="gene ID" value="SSO1639"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1639"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1686"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1302673_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XR9"/>
    <dbReference type="PhylomeDB" id="Q97XR9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="1">
        <location>
            <begin position="92"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="209" mass="24565" checksum="C38BAD975040B8C8" modified="2001-10-01" version="1">MTTLYLFLKDIETKYKNRLKRALFLVSDNDKVNKDRLERLAKGDFTREELYQLEYTPIVLEEIKILAKNLLEIQERLKEVRRMIEGQVPQDHVLLTIPGVGKLAAGIVGDIRRFPKPESFVAYCGLDPVVERSGRAVISRGISKRGNKYLRSLFYFLAVRNYSRNPTLLKFYETHKDRLKGRKLYVALARKLARVVWSVWYNNRPYEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XU4</accession>
    <name>Q97XU4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF973 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1611</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90321">
        <property type="entry name" value="C90321"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923515.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XU4"/>
    <dbReference type="PaxDb" id="273057-SSO1611"/>
    <dbReference type="EnsemblBacteria" id="AAK41826">
        <property type="protein sequence ID" value="AAK41826"/>
        <property type="gene ID" value="SSO1611"/>
    </dbReference>
    <dbReference type="GeneID" id="7798687"/>
    <dbReference type="KEGG" id="sso:SSO1611"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1653"/>
    <dbReference type="eggNOG" id="arCOG03768">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1036727_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XU4"/>
    <dbReference type="PhylomeDB" id="Q97XU4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009321">
        <property type="entry name" value="DUF973"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06157">
        <property type="entry name" value="DUF973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="29615" checksum="534FEDE690F95CF3" modified="2001-10-01" version="1">MESSIQALKNIKTGSLYYFLAIIIALVTEVVILRENSLANSFFVLIFELVAYGILLIGFSRMRTGFFQLSKTINTYIGTTGVNIFIIGIVALLFGAILPILIYIGGSLVFIGDVMIGYTIYKIGQRFQVNTEKIAGILVMISITSFIGYIISYFASDEVMRKMLQAPREEKLLIVPSDKSIGYGILKSNGDANVVVYSKESSEIISAQIMGYNFQALQINPKTVMAGENRIYIKFALSGVTFVPGNIYNLEILLANSEKLLASLVYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XU5</accession>
    <name>Q97XU5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99321">
        <property type="entry name" value="B99321"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923514.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XU5"/>
    <dbReference type="PaxDb" id="273057-SSO1610"/>
    <dbReference type="EnsemblBacteria" id="AAK41825">
        <property type="protein sequence ID" value="AAK41825"/>
        <property type="gene ID" value="SSO1610"/>
    </dbReference>
    <dbReference type="GeneID" id="1454604"/>
    <dbReference type="KEGG" id="sso:SSO1610"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1652"/>
    <dbReference type="eggNOG" id="arCOG08408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147149_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XU5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="148" mass="16357" checksum="886BCBDA866191FF" modified="2001-10-01" version="1">MISMQVKISGNQVLLVNNSYCAEGFLTFNGNTYTIPVPRQFLYSGTPVIFANSDFGGSVNPSFALEGTYTDLFKLFNIISLWSTGWYGMFINGQWVTYINAVVGYSSATGVGDTATIPSYVVESGLTLFHNEFLGVWTNPYVWYFSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XV1</accession>
    <name>Q97XV1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fluoroacetyl-CoA-specific thioesterase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO8469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90320">
        <property type="entry name" value="D90320"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923508.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XV1"/>
    <dbReference type="STRING" id="273057.SSO8469"/>
    <dbReference type="PaxDb" id="273057-SSO8469"/>
    <dbReference type="EnsemblBacteria" id="AAK41819">
        <property type="protein sequence ID" value="AAK41819"/>
        <property type="gene ID" value="SSO8469"/>
    </dbReference>
    <dbReference type="GeneID" id="7810242"/>
    <dbReference type="KEGG" id="sso:SSO8469"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1646"/>
    <dbReference type="eggNOG" id="arCOG04331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2505110_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XV1"/>
    <dbReference type="PhylomeDB" id="Q97XV1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025540">
        <property type="entry name" value="FlK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054485">
        <property type="entry name" value="FlK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36934">
        <property type="entry name" value="BLR0278 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36934:SF1">
        <property type="entry name" value="THIOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22636">
        <property type="entry name" value="FlK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fluoroacetyl-CoA-specific thioesterase-like" evidence="1">
        <location>
            <begin position="15"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="85" mass="9498" checksum="62005B97EB5EA0AE" modified="2001-10-01" version="1">MEKVFLIRQEHSSVVVGSGNVNVLSTPLMIAFMENVALELAQKYLEKGKTTVGYHVDVKHLMPISIGRKLKRATLIEVFNGKESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y28</accession>
    <name>Q97Y28_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1521</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90310">
        <property type="entry name" value="H90310"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y28"/>
    <dbReference type="STRING" id="273057.SSO1521"/>
    <dbReference type="PaxDb" id="273057-SSO1521"/>
    <dbReference type="EnsemblBacteria" id="AAK41743">
        <property type="protein sequence ID" value="AAK41743"/>
        <property type="gene ID" value="SSO1521"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1521"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1562"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1493042_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y28"/>
    <dbReference type="PhylomeDB" id="Q97Y28"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="21097" checksum="BA63D5E0575A558C" modified="2001-10-01" version="1">MYKPTVWLTPKLSYSINFERMTVRIANVGELPILGYPSNLSFYKDWKMKEARLVIKDGKAFLKVVFEKKPVRVEAKGSVVVDINIGEIVVGKDDTHYVRIPTRLSEVHHLKSLAEGLQRKYPRRWRENKHIRARISHFHAKVKRIMEDFAKKVGKWVVEIAEDFNANVTKLERLTNLIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y65</accession>
    <name>Q97Y65_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99306">
        <property type="entry name" value="A99306"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923419.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y65"/>
    <dbReference type="STRING" id="273057.SSO1479"/>
    <dbReference type="PaxDb" id="273057-SSO1479"/>
    <dbReference type="EnsemblBacteria" id="AAK41704">
        <property type="protein sequence ID" value="AAK41704"/>
        <property type="gene ID" value="SSO1479"/>
    </dbReference>
    <dbReference type="GeneID" id="1454483"/>
    <dbReference type="KEGG" id="sso:SSO1479"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1517"/>
    <dbReference type="eggNOG" id="arCOG06508">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134214_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y65"/>
    <dbReference type="PhylomeDB" id="Q97Y65"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase InsH N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="142" mass="16650" checksum="F360C477ABD3C4A9" modified="2001-10-01" version="1">MVKAISTEKDLLLKVDKSFPWETFRSKLKSLYSKKPKWDVILLLKVLLIKFVFDISWNNLEGEIRDSKRFMDFLGGKVPPKSTVFFFYKKLQQTVIQEGETMRTTLMDELNKALDKVISEYREKGFELEVGREKTIGSRTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y72</accession>
    <name>Q97Y72_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90305">
        <property type="entry name" value="B90305"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993099.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y72"/>
    <dbReference type="PaxDb" id="273057-SSO1470"/>
    <dbReference type="DNASU" id="1454476"/>
    <dbReference type="EnsemblBacteria" id="AAK41697">
        <property type="protein sequence ID" value="AAK41697"/>
        <property type="gene ID" value="SSO1470"/>
    </dbReference>
    <dbReference type="GeneID" id="1454476"/>
    <dbReference type="KEGG" id="sso:SSO1470"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1507"/>
    <dbReference type="eggNOG" id="arCOG01192">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153285_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y72"/>
    <dbReference type="PhylomeDB" id="Q97Y72"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="11"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="141" mass="16458" checksum="BC9E80703EDCD864" modified="2001-10-01" version="1">MNSKEIAEGYLREAKIRIKYAELSLNEDKDYAFCVRASQEIVELSIKAILRLLGIEYGKIHDPGKILVENMDRLPSWLKKEAENIAYISRWLRAEREPSMYGDEVEGIPPTELYTESYCRKALESAKYVYELSLKLYNEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YA8</accession>
    <name>Q97YA8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1424</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2023" name="Curr. Res. Struct. Biol." volume="5" first="100098" last="100098">
            <title>Structure of the &lt;i&gt;Saccharolobus solfataricus&lt;/i&gt; type III-D CRISPR effector.</title>
            <authorList>
                <person name="Cannone G."/>
                <person name="Kompaniiets D."/>
                <person name="Graham S."/>
                <person name="White M.F."/>
                <person name="Spagnolo L."/>
            </authorList>
            <dbReference type="PubMed" id="36843655"/>
            <dbReference type="DOI" id="10.1016/j.crstbi.2023.100098"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (3.50 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90300">
        <property type="entry name" value="C90300"/>
    </dbReference>
    <dbReference type="PDB" id="8BMW">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="A/B/C/D/E=1-139"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YA8"/>
    <dbReference type="SMR" id="Q97YA8"/>
    <dbReference type="STRING" id="273057.SSO1424"/>
    <dbReference type="PaxDb" id="273057-SSO1424"/>
    <dbReference type="EnsemblBacteria" id="AAK41658">
        <property type="protein sequence ID" value="AAK41658"/>
        <property type="gene ID" value="SSO1424"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1424"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1450"/>
    <dbReference type="eggNOG" id="arCOG07256">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1954761_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YA8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="3" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="8BMW"/>
        </source>
    </evidence>
    <sequence length="139" mass="15948" checksum="D3E24AC9F5BD4736" modified="2001-10-01" version="1">MRVKHYIQREFNYSVSSQDLLDIATRIAISAIKPKPKSNKPEPYVDSSTINSLLSFLQSRRNVNELLLYIMRQAGRDEIDEETGKLLLASLKDRELKDAVNLLGYVKWVYDTLTGLKVNYNNVKGVKTFKELVNILSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YG3</accession>
    <name>Q97YG3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90292">
        <property type="entry name" value="D90292"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923352.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YG3"/>
    <dbReference type="STRING" id="273057.SSO1360"/>
    <dbReference type="PaxDb" id="273057-SSO1360"/>
    <dbReference type="EnsemblBacteria" id="AAK41595">
        <property type="protein sequence ID" value="AAK41595"/>
        <property type="gene ID" value="SSO1360"/>
    </dbReference>
    <dbReference type="GeneID" id="1454374"/>
    <dbReference type="KEGG" id="sso:SSO1360"/>
    <dbReference type="eggNOG" id="arCOG06000">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870834_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YG3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="136" mass="15248" checksum="04BDEF7202C65546" modified="2001-10-01" version="1">MNLGKIIGLVVFISLLIVQFAILNNPINLVQTSFNQVNSIIIPLFTSEPKIGLIISHNELILTFNNTLDLPITLLNITGEYTYLSKPVTINPNEVKNVSVVVTNFKMFEMAVKDNSYNLTMVIKIFNTTFSQSEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YM7</accession>
    <name>Q97YM7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90284">
        <property type="entry name" value="F90284"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YM7"/>
    <dbReference type="PaxDb" id="273057-SSO1296"/>
    <dbReference type="EnsemblBacteria" id="AAK41533">
        <property type="protein sequence ID" value="AAK41533"/>
        <property type="gene ID" value="SSO1296"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1296"/>
    <dbReference type="HOGENOM" id="CLU_2313923_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YM7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="99" mass="11427" checksum="A9DDD456CB7BB7F7" modified="2001-10-01" version="1">MMLNTLSISGFFQIFTYTIHKSCIKTILVKLSASSTRKRSSGYKKKIVYMYFKKKKNILYSLAVFFLTVGIIDINIIISMNAITIAGIPYTWYNVYFPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YN0</accession>
    <name>Q97YN0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in partial transposon ISC1160</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO7610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK67515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YN0"/>
    <dbReference type="STRING" id="273057.SSO7610"/>
    <dbReference type="PaxDb" id="273057-SSO7610"/>
    <dbReference type="EnsemblBacteria" id="AAK67515">
        <property type="protein sequence ID" value="AAK67515"/>
        <property type="gene ID" value="SSO7610"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7610"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1293"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2730593_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YN0"/>
    <dbReference type="PhylomeDB" id="Q97YN0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK67515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="71" mass="8125" checksum="280E48A8D0E9DDC6" modified="2001-10-01" version="1">MTKDIVKIFVEQVSGNFNISITLDAGFNSIDVLNFILQFKYIIAVPVGDVKIYELMGNTRRTLRGTRRMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YP0</accession>
    <name>Q97YP0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO7578</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90283">
        <property type="entry name" value="C90283"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YP0"/>
    <dbReference type="PaxDb" id="273057-SSO7578"/>
    <dbReference type="DNASU" id="1454301"/>
    <dbReference type="EnsemblBacteria" id="AAK41522">
        <property type="protein sequence ID" value="AAK41522"/>
        <property type="gene ID" value="SSO7578"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7578"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1284"/>
    <dbReference type="eggNOG" id="arCOG08588">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2534836_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YP0"/>
    <dbReference type="PhylomeDB" id="Q97YP0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="83" mass="9557" checksum="EDB59C0E6963E63A" modified="2001-10-01" version="1">MEGEIRDSKMFMDFLGGKIPPKSTVFSFYKKLQQTVVQEGETMRTTLMDELNKALNKVISEYREKGFELEVGREKTIGSRTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YP4</accession>
    <name>Q97YP4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oligo/dipeptide transport, ATP binding protein. amino-end. (DppF-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">dppF-2</name>
        <name evidence="5" type="ordered locus">SSO1281</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90282">
        <property type="entry name" value="G90282"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923284.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YP4"/>
    <dbReference type="FunCoup" id="Q97YP4">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1281"/>
    <dbReference type="PaxDb" id="273057-SSO1281"/>
    <dbReference type="EnsemblBacteria" id="AAK41518">
        <property type="protein sequence ID" value="AAK41518"/>
        <property type="gene ID" value="SSO1281"/>
    </dbReference>
    <dbReference type="GeneID" id="1454297"/>
    <dbReference type="KEGG" id="sso:SSO1281"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1280"/>
    <dbReference type="eggNOG" id="arCOG00204">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="InParanoid" id="Q97YP4"/>
    <dbReference type="PhylomeDB" id="Q97YP4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050319">
        <property type="entry name" value="ABC_transp_ATP-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776:SF8">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776">
        <property type="entry name" value="TRANSPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="8"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="245" mass="27685" checksum="B454E53C0DAFD005" modified="2001-10-01" version="1">MDNNEIIVKVQNVWIKYPMGRVGLFRKLYVHAVNDVSLEIKKGEIFGLIGESGSGKTTLGKGILRLIETYSGSIYWKVDGGKLVDITKLNDNTLRRLRKDFQIIQQDPYGALDPRMSVYEILAEGLRLHKLISNKSEEVEQIYKILSIVKLTPPENFANKKPMELSGGQRQRVVVARALLLRPKFIVADEPISMLDASTRGQILEFILNDRDQRGTAYLFITHDISIASYVSDRIGSGVSPNYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YR0</accession>
    <name>Q97YR0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LOG family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1261</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90280">
        <property type="entry name" value="D90280"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YR0"/>
    <dbReference type="STRING" id="273057.SSO1261"/>
    <dbReference type="PaxDb" id="273057-SSO1261"/>
    <dbReference type="EnsemblBacteria" id="AAK41499">
        <property type="protein sequence ID" value="AAK41499"/>
        <property type="gene ID" value="SSO1261"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1261"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1257"/>
    <dbReference type="eggNOG" id="arCOG02431">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107614_0_1_2"/>
    <dbReference type="InParanoid" id="Q97YR0"/>
    <dbReference type="PhylomeDB" id="Q97YR0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041164">
        <property type="entry name" value="LDcluster4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18306">
        <property type="entry name" value="LDcluster4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102405">
        <property type="entry name" value="MCP/YpsA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="20623" checksum="821616AB77DBF40B" modified="2001-10-01" version="1">MEKGFIILMIISIAAHSEESNHELAEKARKFVRSIKPCNPILLLGGYWGLMRVVVDEALRENIKTVLILPIEKENIEIPREVICIKSGCEFRCRSVILVRSGDLLVSLGGGVGTEIEIMMAYAMGKPIYALTNTGLSTDYFAKSFPDYIDDRKIVKVKYFDNVERMAEEICEDEIKGKEVNFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z30</accession>
    <name>Q97Z30_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90263">
        <property type="entry name" value="H90263"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989743.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z30"/>
    <dbReference type="STRING" id="273057.SSO1110"/>
    <dbReference type="PaxDb" id="273057-SSO1110"/>
    <dbReference type="EnsemblBacteria" id="AAK41367">
        <property type="protein sequence ID" value="AAK41367"/>
        <property type="gene ID" value="SSO1110"/>
    </dbReference>
    <dbReference type="GeneID" id="1454146"/>
    <dbReference type="KEGG" id="sso:SSO1110"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1107"/>
    <dbReference type="eggNOG" id="arCOG02242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150444_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z30"/>
    <dbReference type="PhylomeDB" id="Q97Z30"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="15"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="111" mass="12433" checksum="565B08563B160303" modified="2001-10-01" version="1">MSLKLSESKEVIRCCYKISDTDVECLFKLVELNRPISAEELASIMKLSKTTVENSLKKLIEIGLIVRNKDGEEGKRIGRPKYLYAVIHNAETKIKQDLTNCASKILSATSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z35</accession>
    <name>Q97Z35_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1104</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90263">
        <property type="entry name" value="B90263"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z35"/>
    <dbReference type="STRING" id="273057.SSO1104"/>
    <dbReference type="PaxDb" id="273057-SSO1104"/>
    <dbReference type="EnsemblBacteria" id="AAK41361">
        <property type="protein sequence ID" value="AAK41361"/>
        <property type="gene ID" value="SSO1104"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1104"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1101"/>
    <dbReference type="eggNOG" id="arCOG07291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2217100_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z35"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="115" mass="13645" checksum="FF13FF4A61E3D17E" modified="2001-10-01" version="1">MRRRQKELLDDKEILLSALDKVDKFYVYLAGINSSEILLVTTLNVPNEIEVEGKKFKVVKYHPEDYLSQVVEKEDEIFRKYKIYYFVKAYMRKILDTLSSAEVERMSLDLKDNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z44</accession>
    <name>Q97Z44_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99262">
        <property type="entry name" value="A99262"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989726.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z44"/>
    <dbReference type="PaxDb" id="273057-SSO1092"/>
    <dbReference type="EnsemblBacteria" id="AAK41352">
        <property type="protein sequence ID" value="AAK41352"/>
        <property type="gene ID" value="SSO1092"/>
    </dbReference>
    <dbReference type="GeneID" id="1454131"/>
    <dbReference type="KEGG" id="sso:SSO1092"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1090"/>
    <dbReference type="eggNOG" id="arCOG07290">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1472147_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z44"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="183" mass="21068" checksum="FFA4FF8160263811" modified="2001-10-01" version="1">MKLNGIDISSIIAAETGYIITRYEFIDSFAEEFPAYVSYDLTNNALRKLIIFDPPKVGFNFYPNYKYKIKVKKSAETLYSLKGSDRVLIALKAYKKVIGEMNVLMTKLYFLGLKNGKPYRMLILNDVPIIASDKRELIDKLIGYLKENYNITVSNIPIIVDGIEYRERNDVRILDVDYATIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z67</accession>
    <name>Q97Z67_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD linked oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90258">
        <property type="entry name" value="G90258"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989887.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z67"/>
    <dbReference type="STRING" id="273057.SSO1063"/>
    <dbReference type="PaxDb" id="273057-SSO1063"/>
    <dbReference type="EnsemblBacteria" id="AAK41326">
        <property type="protein sequence ID" value="AAK41326"/>
        <property type="gene ID" value="SSO1063"/>
    </dbReference>
    <dbReference type="GeneID" id="1454105"/>
    <dbReference type="KEGG" id="sso:SSO1063"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1060"/>
    <dbReference type="eggNOG" id="arCOG00345">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_975268_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z67"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD linked oxidase N-terminal" evidence="1">
        <location>
            <begin position="10"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="304" mass="34929" checksum="7F8FCF649E938822" modified="2001-10-01" version="1">MIKIIFVLEVNPADENEVFQIISESKKDNKKVGIRGFGSHTKREIKPDIMIITTEKLNNFEISENKVIADSGADVKKIREEALQKGLLFPSIYDGSIGGLLALNEMSSISTAYGTPWNFTEWVDFINTFGKIRWRAVIGSQGLFGVITKASLKLYEKPSKVFIYEREITDKKELKLELRKLIGLKPIALLVEYEGDNKAFEIHASYVTQYDLQSYSKDEGIPMISEVSDKNSYIVEISDFVEDFISIAERVSPYYMYGIYGVNLLKVYVTDDSLLRDLKYYPRNKPHPVFYKLKRVLDFYNIFA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z97</accession>
    <name>Y1033_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Kelch domain-containing protein SSO1033</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO1033</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90255">
        <property type="entry name" value="A90255"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989202.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z97"/>
    <dbReference type="SMR" id="Q97Z97"/>
    <dbReference type="STRING" id="273057.SSO1033"/>
    <dbReference type="PaxDb" id="273057-SSO1033"/>
    <dbReference type="EnsemblBacteria" id="AAK41296">
        <property type="protein sequence ID" value="AAK41296"/>
        <property type="gene ID" value="SSO1033"/>
    </dbReference>
    <dbReference type="GeneID" id="84061512"/>
    <dbReference type="KEGG" id="sso:SSO1033"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1025"/>
    <dbReference type="eggNOG" id="arCOG05978">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_385724_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z97"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF166">
        <property type="entry name" value="NEURONAL IGCAM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0880">Kelch repeat</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000016653" description="Kelch domain-containing protein SSO1033">
        <location>
            <begin position="29"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="repeat" description="Kelch 1">
        <location>
            <begin position="59"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="Kelch 2">
        <location>
            <begin position="101"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="repeat" description="Kelch 3">
        <location>
            <begin position="146"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="Kelch 4">
        <location>
            <begin position="201"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="Kelch 5">
        <location>
            <begin position="250"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="repeat" description="Kelch 6">
        <location>
            <begin position="299"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III 1" evidence="2">
        <location>
            <begin position="323"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III 2" evidence="2">
        <location>
            <begin position="411"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III 3" evidence="2">
        <location>
            <begin position="505"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III 4" evidence="2">
        <location>
            <begin position="585"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00316"/>
        </source>
    </evidence>
    <sequence length="725" mass="78466" checksum="FE8F1220D013BAB6" modified="2001-10-01" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZD8</accession>
    <name>Q97ZD8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90249">
        <property type="entry name" value="G90249"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923157.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZD8"/>
    <dbReference type="STRING" id="273057.SSO0980"/>
    <dbReference type="PaxDb" id="273057-SSO0980"/>
    <dbReference type="EnsemblBacteria" id="AAK41254">
        <property type="protein sequence ID" value="AAK41254"/>
        <property type="gene ID" value="SSO0980"/>
    </dbReference>
    <dbReference type="GeneID" id="1455221"/>
    <dbReference type="KEGG" id="sso:SSO0980"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.979"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077283_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZD8"/>
    <dbReference type="PhylomeDB" id="Q97ZD8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="354" mass="40227" checksum="ECF4FF90FABED498" modified="2001-10-01" version="1">MTKELTREEYYKALEKAVNEVILSMTGTRKDVAKRLVLGAVVGRNATEIAQEAEMDYETVLNNLDKAAQAKLIEVVKKLVGDHPVLLIVDDTHDHKLYARAMPVSRNGAQIFYCRSHKRFEPAIQLLVIGVKDLVNNQIYVIHIIAYIPRKVEEELKRRGEEVKFKTKIDALLEFLSSLSGLNVKAKVFDSWYVNSRTLQGNTVGELKSSARVVEGGRSVPVSEFPQGEYLVEYLGTPIKLLVIDDYKGYGRRYFFSTDLNDTAEDIITTWENRWDIEVLIRELKALGLEGGSFLTWVRNSGFVALKALSLLVVQYFKYSTGLVLGAKRLARLIKSIYHEVGGIKKLFKRRRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZE4</accession>
    <name>Q97ZE4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-oxoacyl-(Acyl carrier protein) reductase (FabG-1)</fullName>
            <ecNumber evidence="2">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fabG-1</name>
        <name evidence="2" type="ordered locus">SSO0975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99249">
        <property type="entry name" value="A99249"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992424.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZE4"/>
    <dbReference type="FunCoup" id="Q97ZE4">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0975"/>
    <dbReference type="PaxDb" id="273057-SSO0975"/>
    <dbReference type="EnsemblBacteria" id="AAK41248">
        <property type="protein sequence ID" value="AAK41248"/>
        <property type="gene ID" value="SSO0975"/>
    </dbReference>
    <dbReference type="GeneID" id="1455215"/>
    <dbReference type="KEGG" id="sso:SSO0975"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.973"/>
    <dbReference type="eggNOG" id="arCOG01260">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_2"/>
    <dbReference type="InParanoid" id="Q97ZE4"/>
    <dbReference type="PhylomeDB" id="Q97ZE4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05344">
        <property type="entry name" value="BKR_like_SDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050259">
        <property type="entry name" value="SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879:SF5">
        <property type="entry name" value="SHORT-CHAIN ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="265" mass="29674" checksum="3BF920533E4E192C" modified="2001-10-01" version="1">MNINISGKRVLVTASTEGIGRGIAEAFLREGCNVVISSRNKDKIEKAILEMRKIGPSVWGFPADLTDFKSLEELVGYALKIMNGIDILVVNSGNPPKEPSYFFENTMEDWEYATKLYLLSAIKLTNLVYQYMKAQRWGRIFFLSSWTIKEPQRIFSLADITRAPLIQMAKLLSKELGEYNVNVNVILMGSFETEGAKRSLKKYAEKVGQPLDVIWKREVISQIPIGRTGDIKNELGSLLVFLSSDYAGYINGTSILIDGGMSRAI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZI6</accession>
    <name>GCSH1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable glycine cleavage system H protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gcvH1</name>
        <name type="ordered locus">SSO0920</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="1">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90243">
        <property type="entry name" value="C90243"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992352.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZI6"/>
    <dbReference type="SMR" id="Q97ZI6"/>
    <dbReference type="FunCoup" id="Q97ZI6">
        <property type="interactions" value="245"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0920"/>
    <dbReference type="PaxDb" id="273057-SSO0920"/>
    <dbReference type="EnsemblBacteria" id="AAK41202">
        <property type="protein sequence ID" value="AAK41202"/>
        <property type="gene ID" value="SSO0920"/>
    </dbReference>
    <dbReference type="GeneID" id="1455169"/>
    <dbReference type="KEGG" id="sso:SSO0920"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.921"/>
    <dbReference type="eggNOG" id="arCOG01303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097408_2_2_2"/>
    <dbReference type="InParanoid" id="Q97ZI6"/>
    <dbReference type="PhylomeDB" id="Q97ZI6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017453">
        <property type="entry name" value="GCV_H_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00527">
        <property type="entry name" value="gcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002270">
        <property type="entry name" value="PRK01202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715:SF3">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0450">Lipoyl</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000166282" description="Probable glycine cleavage system H protein 1">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="2">
        <location>
            <begin position="31"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="1">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01066"/>
        </source>
    </evidence>
    <sequence length="137" mass="15345" checksum="37F78CDD00BE38C8" modified="2001-10-01" version="1">MNEMKVGRYVVLTDRLYTETDEWVVLSNDNVAVIGITDYAQKKLRDIVGIELPQLQKEVKAGESVGVIESVKAAADIFSPLSGIIVEVNNKLLEHPEIINKDPYGEGWIFKLKASKLSEEKEKLLSPEKYIEKIKGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-03" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZI8</accession>
    <name>GCSPA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable glycine dehydrogenase (decarboxylating) subunit 1</fullName>
            <ecNumber evidence="1">1.4.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glycine cleavage system P-protein subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycine decarboxylase subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycine dehydrogenase (aminomethyl-transferring) subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gcvPA</name>
        <name type="ordered locus">SSO0918</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvP family. N-terminal subunit subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99243">
        <property type="entry name" value="A99243"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992350.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZI8"/>
    <dbReference type="SMR" id="Q97ZI8"/>
    <dbReference type="FunCoup" id="Q97ZI8">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0918"/>
    <dbReference type="PaxDb" id="273057-SSO0918"/>
    <dbReference type="EnsemblBacteria" id="AAK41200">
        <property type="protein sequence ID" value="AAK41200"/>
        <property type="gene ID" value="SSO0918"/>
    </dbReference>
    <dbReference type="GeneID" id="1455167"/>
    <dbReference type="GeneID" id="44129848"/>
    <dbReference type="KEGG" id="sso:SSO0918"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.919"/>
    <dbReference type="eggNOG" id="arCOG00077">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004620_0_2_2"/>
    <dbReference type="InParanoid" id="Q97ZI8"/>
    <dbReference type="PhylomeDB" id="Q97ZI8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00712">
        <property type="entry name" value="GcvPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023010">
        <property type="entry name" value="GcvPA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001696">
        <property type="entry name" value="PRK00451.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42806">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM P-PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42806:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006815">
        <property type="entry name" value="GcvPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000166989" description="Probable glycine dehydrogenase (decarboxylating) subunit 1">
        <location>
            <begin position="1"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00712"/>
        </source>
    </evidence>
    <sequence length="455" mass="51367" checksum="510B48D12A705EAA" modified="2001-10-01" version="1">MYKHPWLPNLDLTDEMLKEIGVNSLDDLFNDIPAEIKINRLLKVAKDKPLSEYEIEKEIYEKVKKNVELEAPPFIGAGICPHYIPNAVKFIIGRSEFYTSYTPYQPEISQGILQALFEYQSLMAELLEMDVVNASMYDWGSALAEAVLMANRINGKKTVLVPENANPFHKEVMRTWIQGKGIKIEEVKYDKNSGEVDIEDLEKKSSSNDVSAIYVQQPNFFGIFESNIEHIIDVAKHKKALSIIGVNPLSLGLIKPPGSYEADIVVGDGQELGLPLNFGGPLMGIFAVRWDMGLVRQMPGRIVGITRDVNNNMGFTLILQTREQFIKREKATSNITTNEALLALANAVYLSLLGKEGIRELAEEIYFRSHYAAKRLTEIDNVIMPFTSDFFEEFVIKFPIEYNIINDKLKERKLQGGLKLSIHTSLFCVTEVHDKKSIDLLVSTIQEAIKSVETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZM7</accession>
    <name>Q97ZM7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0863</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99237">
        <property type="entry name" value="B99237"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992284.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZM7"/>
    <dbReference type="PaxDb" id="273057-SSO0863"/>
    <dbReference type="EnsemblBacteria" id="AAK41153">
        <property type="protein sequence ID" value="AAK41153"/>
        <property type="gene ID" value="SSO0863"/>
    </dbReference>
    <dbReference type="GeneID" id="1455120"/>
    <dbReference type="KEGG" id="sso:SSO0863"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.874"/>
    <dbReference type="eggNOG" id="arCOG05984">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1127132_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZM7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="246" mass="29305" checksum="37DD9EC061AC4E2A" modified="2001-10-01" version="1">MMCERCNKRNAINVVGGRRLCEICSKDEITKRIKRELYPRKIIVYKDKILFAYPSYLSFIREILKNIINKIYAKFNLQYYEITLEPQHSILDDMWNLIVKSKRFSEENGISKIFLPFTADFLMAYLIYSITNQDYTYIKMIGLEYNVNNISFLIPFYNTSLYELQGFFTNESNNIFETKDEIFNEILAWERDTLKENYELFHAFHNSKKLFETKEKEYRCEGCGGVINSPVKYCARCSLIYSSPPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZN9</accession>
    <name>Q97ZN9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0845</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90235">
        <property type="entry name" value="D90235"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZN9"/>
    <dbReference type="STRING" id="273057.SSO0845"/>
    <dbReference type="PaxDb" id="273057-SSO0845"/>
    <dbReference type="EnsemblBacteria" id="AAK41139">
        <property type="protein sequence ID" value="AAK41139"/>
        <property type="gene ID" value="SSO0845"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0845"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.861"/>
    <dbReference type="eggNOG" id="arCOG07730">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1232811_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZN9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="224" mass="27012" checksum="601D26083592DEE1" modified="2001-10-01" version="1">MHSIRMGLLGDMRLYLDIETYRPKSAFVNEKVIAIGLIADQTDYKPESSNIWDLPKVEFKYFKEWKRDERSEEWEHDEYSVVTQFYKYLKELIEKWRRKEMYIEVVGFNILRFDIPLLIQKGVEHRVGSLEELNSLWHNTFTRDYLQMALPLNNMRFKGLQLEYLAEMLREKGVNVPKPYGKGEEVKSLYENKKYDEIIKHLEADLRITRIIDLNWPRLFKPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZQ8</accession>
    <name>Q97ZQ8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphoribosylformylglycinamidine synthase subunit PurS</fullName>
            <shortName evidence="6">FGAM synthase</shortName>
            <ecNumber evidence="6">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Formylglycinamide ribonucleotide amidotransferase subunit III</fullName>
            <shortName evidence="6">FGAR amidotransferase III</shortName>
            <shortName evidence="6">FGAR-AT III</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosylformylglycinamidine synthase subunit III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">purS</name>
        <name evidence="7" type="ordered locus">SSO6264</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PurS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90210">
        <property type="entry name" value="C90210"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991163.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZQ8"/>
    <dbReference type="STRING" id="273057.SSO6264"/>
    <dbReference type="PaxDb" id="273057-SSO6264"/>
    <dbReference type="EnsemblBacteria" id="AAK40938">
        <property type="protein sequence ID" value="AAK40938"/>
        <property type="gene ID" value="SSO6264"/>
    </dbReference>
    <dbReference type="GeneID" id="1454905"/>
    <dbReference type="GeneID" id="44129629"/>
    <dbReference type="KEGG" id="sso:SSO6264"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.634"/>
    <dbReference type="eggNOG" id="arCOG04462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164833_2_0_2"/>
    <dbReference type="InParanoid" id="Q97ZQ8"/>
    <dbReference type="PhylomeDB" id="Q97ZQ8"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1280.10">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase subunit PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01926">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003850">
        <property type="entry name" value="PurS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00302">
        <property type="entry name" value="phosphoribosylformylglycinamidine synthase subunit PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004450">
        <property type="entry name" value="PRK05783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02700">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="PurS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="84" mass="10039" checksum="AD728F5162E511ED" modified="2001-10-01" version="1">MKYYVELIIINKDSVRDPEGETIQKYVIEKYTNNIIETRVGKYIQFKIEANNEEDAKNLIEKIANEGRLYNPIIHKILVRVRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZY2</accession>
    <name>Q97ZY2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphate transport regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90188">
        <property type="entry name" value="F90188"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988731.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZY2"/>
    <dbReference type="STRING" id="273057.SSO0440"/>
    <dbReference type="PaxDb" id="273057-SSO0440"/>
    <dbReference type="EnsemblBacteria" id="AAK40765">
        <property type="protein sequence ID" value="AAK40765"/>
        <property type="gene ID" value="SSO0440"/>
    </dbReference>
    <dbReference type="GeneID" id="1455575"/>
    <dbReference type="KEGG" id="sso:SSO0440"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.433"/>
    <dbReference type="eggNOG" id="arCOG02640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1280848_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZY2"/>
    <dbReference type="PhylomeDB" id="Q97ZY2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="24851" checksum="EF095CEFA6B87E53" modified="2001-10-01" version="1">MGEGIATLAIEEQLQQISLKLLDETRVLYEFLSNTSNSNVNAMQIYSKVNGIKNDIEVSKYRLGEYIFKIREGLLDKDLYVEILNNLEKVSQNLDAATYRLSVILSKQMNIDNVINRLLIVICEKIITSITYFIEALRLLSVNPKNSYENARNVIKLEQEIDELYRSLELALFEKKLADFSYIMLLKDIADRLEDSEDLLKSSADNITYIAYERM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q980H1</accession>
    <name>Q980H1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH dehydrogenase subunit I (NuoI)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NuoI</name>
        <name evidence="7" type="ordered locus">SSO0326</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90175">
        <property type="entry name" value="F90175"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980H1"/>
    <dbReference type="FunCoup" id="Q980H1">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0326"/>
    <dbReference type="PaxDb" id="273057-SSO0326"/>
    <dbReference type="EnsemblBacteria" id="AAK40661">
        <property type="protein sequence ID" value="AAK40661"/>
        <property type="gene ID" value="SSO0326"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0326"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.320"/>
    <dbReference type="eggNOG" id="arCOG01543">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067218_4_5_2"/>
    <dbReference type="InParanoid" id="Q980H1"/>
    <dbReference type="PhylomeDB" id="Q980H1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3270:FF:000014">
        <property type="entry name" value="NADH dehydrogenase subunit I (NuoI)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01971">
        <property type="entry name" value="NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004541">
        <property type="entry name" value="PRK05888.2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849:SF35">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="107"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="188" mass="21435" checksum="4281AF9BF2CE6AEB" modified="2001-10-01" version="1">MEIFLSLSFSFLIIRCGGGLFMDKVKEYKKENIASLFAEHIQSIVTGIKYFVKPQRITLQYPEDSLTLPTGYRGMIRLYKDVCIGCTLCALICPADAMKMVTESGKKFPQINYGRCVFCGFCVDVCPVDALKETKVHDLVFNNRKQLIFDPDRFNVDIDDVPMQDKPVRKVKAIIDEKRGIKYVPADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q980S0</accession>
    <name>Q980S0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme A biosynthesis bifunctional protein CoaBC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA/pantothenate metabolism flavoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphopantothenoylcysteine synthetase/decarboxylase</fullName>
            <shortName evidence="3">PPCS-PPCDC</shortName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenoylcysteine decarboxylase</fullName>
                <shortName evidence="3">PPC decarboxylase</shortName>
                <shortName evidence="3">PPC-DC</shortName>
                <ecNumber evidence="3">4.1.1.36</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaC</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenate--cysteine ligase</fullName>
                <ecNumber evidence="3">6.3.2.5</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaB</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="3">Phosphopantothenoylcysteine synthetase</fullName>
                <shortName evidence="3">PPC synthetase</shortName>
                <shortName evidence="3">PPC-S</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="6" type="primary">dfp</name>
        <name evidence="3" type="synonym">coaBC</name>
        <name evidence="6" type="ordered locus">SSO0210</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(R)-4'-phosphopantothenate + L-cysteine + CTP = N-[(R)-4-phosphopantothenoyl]-L-cysteine + CMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19397"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="6.3.2.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>N-[(R)-4-phosphopantothenoyl]-L-cysteine + H(+) = (R)-4'-phosphopantetheine + CO2</text>
            <dbReference type="Rhea" id="RHEA:16793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="4.1.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the PPC synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.36" evidence="3"/>
    <dbReference type="EC" id="6.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90162">
        <property type="entry name" value="B90162"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990456.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980S0"/>
    <dbReference type="FunCoup" id="Q980S0">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0210"/>
    <dbReference type="PaxDb" id="273057-SSO0210"/>
    <dbReference type="EnsemblBacteria" id="AAK40553">
        <property type="protein sequence ID" value="AAK40553"/>
        <property type="gene ID" value="SSO0210"/>
    </dbReference>
    <dbReference type="GeneID" id="1455363"/>
    <dbReference type="GeneID" id="44129178"/>
    <dbReference type="KEGG" id="sso:SSO0210"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.207"/>
    <dbReference type="eggNOG" id="arCOG01704">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033319_0_3_2"/>
    <dbReference type="InParanoid" id="Q980S0"/>
    <dbReference type="PhylomeDB" id="Q980S0"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02225">
        <property type="entry name" value="CoaBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/pantothenate metabolism flavoprotein C-terminal" evidence="5">
        <location>
            <begin position="195"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenoylcysteine decarboxylase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenate--cysteine ligase" evidence="3">
        <location>
            <begin position="200"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="413" mass="45864" checksum="79569D38D49A5219" modified="2001-10-01" version="1">MHTYMTHPSKKIIGSISNELADKKILLAVTGSVAIYKSLDLARSLMRNGAEVSVIISKDAAKLISPEMFKWATGNNVVTKLTGDLEHVSLAEDNDVMIVAPSTANTMVKIAYGIADTPITATALNFVGMKKPLIIVPSMHLQMYISPQVADAADRLKRIGVEVIEPEIVGDLAHYPKLEYLTSRITSYVLRGKDLSGFNILATAGPTREYLDSVRFISNPSSGTMGISIANEAYFRGAKVRVICGPISSKIEPYVKDIVYVETTEEMLNEVVKSIENGKFNVVILAGAPADYKFKNKSDTKIDSHTEIPKVELERTPKISEYIRKYNVLLVGFSAETVNSDEELIEKAKIKMRRHGFDLIVANNVRRKDIGFSSEYNEVIVIDKNGDVRKIPKNFKTVIARKILDIVKEQLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q980T9</accession>
    <name>Q980T9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn-dependent metallo-hydrolase RNA specificity domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0188</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90159">
        <property type="entry name" value="G90159"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990426.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980T9"/>
    <dbReference type="STRING" id="273057.SSO0188"/>
    <dbReference type="PaxDb" id="273057-SSO0188"/>
    <dbReference type="EnsemblBacteria" id="AAK40534">
        <property type="protein sequence ID" value="AAK40534"/>
        <property type="gene ID" value="SSO0188"/>
    </dbReference>
    <dbReference type="GeneID" id="1455344"/>
    <dbReference type="KEGG" id="sso:SSO0188"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.187"/>
    <dbReference type="eggNOG" id="arCOG00545">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050517_0_0_2"/>
    <dbReference type="InParanoid" id="Q980T9"/>
    <dbReference type="PhylomeDB" id="Q980T9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF51">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn-dependent metallo-hydrolase RNA specificity" evidence="3">
        <location>
            <begin position="274"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="328" mass="37382" checksum="490374C28BCCF74B" modified="2001-10-01" version="1">MQSLVKILPNGAILIGKRFTIDGHHERPFRVVTHFHADHITGLEKSISISDGIIATPITLDILSLDYAIPPRKAFGLNYDIKMTFEDENIVLKKSDHVIGSAQVLITLENGLEIGYTGDFKNPGKGTPILHPDILIIEATYGRPDFRRPFKDDVESLFADYVRDALMYGPVRIYGYHGKLQEVMISLRKMGVDAPFIVGGKISKMTNIAIKYGYNISQVFDESQSEAKEIMRDSWYISFSHYNEFKRRNGKYYNFLLSGWEFKNVVKKIDEKSYTVSFSDHADFDDLIYYVERTSAKYIITDGGRRSYGKELAEYISKKLGKIAISMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q980U0</accession>
    <name>Q980U0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sarcosine oxidase, subunit beta (SoxB-like)</fullName>
            <ecNumber evidence="3">1.5.3.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">soxB-like</name>
        <name evidence="3" type="ordered locus">SSO0187</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.5.3.1" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90159">
        <property type="entry name" value="F90159"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990425.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980U0"/>
    <dbReference type="SMR" id="Q980U0"/>
    <dbReference type="FunCoup" id="Q980U0">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0187"/>
    <dbReference type="PaxDb" id="273057-SSO0187"/>
    <dbReference type="DNASU" id="1455343"/>
    <dbReference type="EnsemblBacteria" id="AAK40533">
        <property type="protein sequence ID" value="AAK40533"/>
        <property type="gene ID" value="SSO0187"/>
    </dbReference>
    <dbReference type="GeneID" id="1455343"/>
    <dbReference type="KEGG" id="sso:SSO0187"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.186"/>
    <dbReference type="eggNOG" id="arCOG00755">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_4_1_2"/>
    <dbReference type="InParanoid" id="Q980U0"/>
    <dbReference type="PhylomeDB" id="Q980U0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008115">
        <property type="term" value="F:sarcosine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF287">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="372" mass="42588" checksum="9021F6C08C30CB25" modified="2001-10-01" version="1">MIIIVGAGSHGLSLAYHLVKKGYSKKDITLVEWKRIGYGSSSRNAARYRYHFYSKENVEYAINAISYLKKQSKELEYNSLLLRTGYLWVINGEQEYSLFKKLDSFWSSYNIGGKFIECKDNYAYIKFEGQCYLAPQDGAFHHDYILYSYYYSIKDKVSTIYDKVVSLIKDDSKIKGVKLSSGKELYGDKVIVTAGAWSGEVLSSIGIEVPIFPEKKEIYITEPLRYFLEPLIIDSKLQIYFSQTLKGEIIGGIEDKREYKFEEFTISISNAIKFLKGIRELIKGIDGIGILRGWSGYYEMTPDSSQIMGYSNDWPEGLFIDAGYSGHGMMFAPYSGKIMADLIADNYKNKFIDIFSPSRFKLNKLINETLVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-03" modified="2026-01-28" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>Q980U7</accession>
    <name>HEM1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA reductase</fullName>
            <shortName evidence="1">GluTR</shortName>
            <ecNumber evidence="1">1.2.1.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemA</name>
        <name type="ordered locus">SSO0180</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-4-amino-5-oxopentanoate + tRNA(Glu) + NADP(+) = L-glutamyl-tRNA(Glu) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12344"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="EC" id="1.2.1.70"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamyl-tRNA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.70" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90158">
        <property type="entry name" value="G90158"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990412.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980U7"/>
    <dbReference type="SMR" id="Q980U7"/>
    <dbReference type="FunCoup" id="Q980U7">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0180"/>
    <dbReference type="PaxDb" id="273057-SSO0180"/>
    <dbReference type="DNASU" id="1455336"/>
    <dbReference type="EnsemblBacteria" id="AAK40526">
        <property type="protein sequence ID" value="AAK40526"/>
        <property type="gene ID" value="SSO0180"/>
    </dbReference>
    <dbReference type="GeneID" id="1455336"/>
    <dbReference type="KEGG" id="sso:SSO0180"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.178"/>
    <dbReference type="eggNOG" id="arCOG01036">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035113_0_0_2"/>
    <dbReference type="InParanoid" id="Q980U7"/>
    <dbReference type="PhylomeDB" id="Q980U7"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00316"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008883">
        <property type="term" value="F:glutamyl-tRNA reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019353">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process from glutamate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05213">
        <property type="entry name" value="NAD_bind_Glutamyl_tRNA_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.30">
        <property type="entry name" value="Glutamyl-tRNA reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00087">
        <property type="entry name" value="Glu_tRNA_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000343">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015896">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015895">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018214">
        <property type="entry name" value="GluRdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036453">
        <property type="entry name" value="GluRdtase_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036343">
        <property type="entry name" value="GluRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000751">
        <property type="entry name" value="PRK00045.4-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000752">
        <property type="entry name" value="PRK00045.4-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43013">
        <property type="entry name" value="GLUTAMYL-TRNA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43013:SF1">
        <property type="entry name" value="GLUTAMYL-TRNA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00745">
        <property type="entry name" value="GlutR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05201">
        <property type="entry name" value="GlutR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000445">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69742">
        <property type="entry name" value="Glutamyl tRNA-reductase catalytic, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69075">
        <property type="entry name" value="Glutamyl tRNA-reductase dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00747">
        <property type="entry name" value="GLUTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000114110" description="Glutamyl-tRNA reductase">
        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="52"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="115"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="190"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for activity" evidence="1">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00087"/>
        </source>
    </evidence>
    <sequence length="426" mass="49422" checksum="7A7CE6CAF76C54EF" modified="2001-10-01" version="1">MSKDEELLQNYCSILFTYKTIGISNLHLYYFRETEIKSLKQLINAEFAILQTCNRVEIYLYSDTNTLKEVNKIIQYLNNIHNEPIGNQARVLCGKDAAKHLFLVASGADSLSIGEYEILSQIRSTIDMFKKLGFSGKYLQIFFERAIKVGRKVREETSISKGKVGIYSLAIDEAKKRFNDFYDRRILVIGAGEMAQKITSMLHNEGAKDVTIMNRTIEKAKQLALKFGYNYEKLDLDKLGNFDVAFISIYHENLRLENKWNTLIVDITVPPLFTGNNVITLEELERISNLNFKAREEELAKINKLVEDGINELLYDYKKEIYTEFMSKIMKRIETIRENEILRAYKELEKLGINDQQAKEILDLMTRSIIKKSFQPLFDNIRSLIFNGENSINYINFLIDIFKDGNISGFETEKIKEKQVSERSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q981B0</accession>
    <name>Q981B0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar hook-basal body protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90143">
        <property type="entry name" value="C90143"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989601.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981B0"/>
    <dbReference type="STRING" id="273057.SSO0037"/>
    <dbReference type="PaxDb" id="273057-SSO0037"/>
    <dbReference type="EnsemblBacteria" id="AAK40402">
        <property type="protein sequence ID" value="AAK40402"/>
        <property type="gene ID" value="SSO0037"/>
    </dbReference>
    <dbReference type="GeneID" id="1455293"/>
    <dbReference type="KEGG" id="sso:SSO0037"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.38"/>
    <dbReference type="eggNOG" id="arCOG07214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_477883_0_0_2"/>
    <dbReference type="InParanoid" id="Q981B0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="575" mass="64035" checksum="317C85FAF6A4FEC0" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX93</accession>
    <name>RL5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl5</name>
        <name evidence="1" type="synonym">rpl5Ab</name>
        <name type="ordered locus">SSO0704</name>
        <name type="ORF">C10_026</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. May contact the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; contacts the 5S rRNA and probably tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90218">
        <property type="entry name" value="G90218"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX93"/>
    <dbReference type="SMR" id="Q9UX93"/>
    <dbReference type="FunCoup" id="Q9UX93">
        <property type="interactions" value="216"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0704"/>
    <dbReference type="PaxDb" id="273057-SSO0704"/>
    <dbReference type="EnsemblBacteria" id="AAK41006">
        <property type="protein sequence ID" value="AAK41006"/>
        <property type="gene ID" value="SSO0704"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0704"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.705"/>
    <dbReference type="eggNOG" id="arCOG04092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061015_3_0_2"/>
    <dbReference type="InParanoid" id="Q9UX93"/>
    <dbReference type="PhylomeDB" id="Q9UX93"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
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    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_A">
        <property type="entry name" value="Ribosomal_uL5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022804">
        <property type="entry name" value="Ribosomal_uL5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057266">
        <property type="entry name" value="Ribosomal_uL5_euk/arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003258">
        <property type="entry name" value="PRK04219.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000125069" description="Large ribosomal subunit protein uL5">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="182" mass="20652" checksum="3070C4B01860D448" modified="2000-05-01" version="1">MIKMAESTLPLSQNPMRKVKLSKVTVNIGLGESGDRLQKAYQLLQELTGAKPVYTISKKTIREFGIRKGQEIGVKVTLRGAQAEEFLRKVLEAIGYRLKKSSFDDYGNVSFGIAEHVILPGARYDPEIGIFGMDIAITLERAGYRISRRRRKKAKIPKRHRVTKEEAMEFLRNNFNVQLVEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-20" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q9V2S9</accession>
    <name>THSA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chaperonin subunit alpha</fullName>
            <ecNumber evidence="1">5.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Thermophilic factor 55 alpha</fullName>
            <shortName>TF55-alpha</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Thermosome subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">thsA</name>
        <name type="ordered locus">SSO0862</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1999" name="Curr. Biol." volume="9" first="1053" last="1056">
            <title>Recurrent paralogy in the evolution of archaeal chaperonins.</title>
            <authorList>
                <person name="Archibald J.M."/>
                <person name="Logsdon J.M. Jr."/>
                <person name="Doolittle W.F."/>
            </authorList>
            <dbReference type="PubMed" id="10508614"/>
            <dbReference type="DOI" id="10.1016/S0960-9822(99)80457-6"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="3">
        <citation type="journal article" date="2016" name="Structure" volume="24" first="364" last="374">
            <title>Structural and Functional Insights into the Evolution and Stress Adaptation of Type II Chaperonins.</title>
            <authorList>
                <person name="Chaston J.J."/>
                <person name="Smits C."/>
                <person name="Aragao D."/>
                <person name="Wong A.S."/>
                <person name="Ahsan B."/>
                <person name="Sandin S."/>
                <person name="Molugu S.K."/>
                <person name="Molugu S.K."/>
                <person name="Bernal R.A."/>
                <person name="Stock D."/>
                <person name="Stewart A.G."/>
            </authorList>
            <dbReference type="PubMed" id="26853941"/>
            <dbReference type="DOI" id="10.1016/j.str.2015.12.016"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS)</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; binds unfolded polypeptides and stimulates protein folding in a nucleotide-hydrolysis-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + an unfolded polypeptide = ADP + phosphate + a folded polypeptide.</text>
            <dbReference type="EC" id="5.6.1.7"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text evidence="3">Thermostable, it unfolds at 94 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="3">The 3 paralogous subunits (alpha, beta and gamma) form oligomeric ring complexes, composition is temperature dependent. Gamma is unstable at 75 degrees Celsius, at 80 degrees Celsius in vivo there are 94-95% alpha-beta complexes with 5-6% beta-only rings, and no detectable gamma-containing rings. In the beta subunit-only complex 2 rings of 9 subunits each stack back-to-back to form an octadecameric complex. In the mixed alpha-beta complex there are 2 stacked 8-membered rings with alternating alpha and beta subunits making a hexadecameric complex; in the stacked rings alpha subunits stack on the beta subunits and only the beta subunits bind ADP. Does not form complexes by itself, but with the beta subunit or the beta and gamma subunits in the presence of ATP; the gamma-containing complexes have 2 rings of 9 subunits making an octadecameric complex.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-16195227">
            <id>Q9V2S9</id>
        </interactant>
        <interactant intactId="EBI-16195251">
            <id>Q9V2T8</id>
            <label>thsB</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>7</experiments>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.7" evidence="1"/>
    <dbReference type="EMBL" id="AF181261">
        <property type="protein sequence ID" value="AAD56682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99237">
        <property type="entry name" value="A99237"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992283.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="4XCG">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.74 A"/>
        <property type="chains" value="A=1-559"/>
    </dbReference>
    <dbReference type="PDB" id="4XCI">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="A=1-559"/>
    </dbReference>
    <dbReference type="PDBsum" id="4XCG"/>
    <dbReference type="PDBsum" id="4XCI"/>
    <dbReference type="AlphaFoldDB" id="Q9V2S9"/>
    <dbReference type="SMR" id="Q9V2S9"/>
    <dbReference type="DIP" id="DIP-61906N"/>
    <dbReference type="FunCoup" id="Q9V2S9">
        <property type="interactions" value="270"/>
    </dbReference>
    <dbReference type="IntAct" id="Q9V2S9">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0862"/>
    <dbReference type="PaxDb" id="273057-SSO0862"/>
    <dbReference type="EnsemblBacteria" id="AAK41152">
        <property type="protein sequence ID" value="AAK41152"/>
        <property type="gene ID" value="SSO0862"/>
    </dbReference>
    <dbReference type="GeneID" id="1455119"/>
    <dbReference type="GeneID" id="44129799"/>
    <dbReference type="KEGG" id="sso:SSO0862"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.873"/>
    <dbReference type="eggNOG" id="arCOG01257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_2"/>
    <dbReference type="InParanoid" id="Q9V2S9"/>
    <dbReference type="PhylomeDB" id="Q9V2S9"/>
    <dbReference type="EvolutionaryTrace" id="Q9V2S9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000128401" description="Chaperonin subunit alpha">
        <location>
            <begin position="1"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="535"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="535"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAD56682." evidence="5" ref="1">
        <original>T</original>
        <variation>A</variation>
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAD56682." evidence="5" ref="1">
        <original>M</original>
        <variation>R</variation>
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="46"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="55"/>
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    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="61"/>
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    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="73"/>
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    <feature type="strand" evidence="8">
        <location>
            <begin position="89"/>
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    <feature type="helix" evidence="8">
        <location>
            <begin position="93"/>
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    <feature type="helix" evidence="8">
        <location>
            <begin position="117"/>
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    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="149"/>
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    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="154"/>
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    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="179"/>
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    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="194"/>
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    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="396"/>
            <end position="408"/>
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    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="412"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="415"/>
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    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="418"/>
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    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="437"/>
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    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="451"/>
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    <feature type="helix" evidence="8">
        <location>
            <begin position="464"/>
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    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="483"/>
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    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="486"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="490"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="495"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="501"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="504"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="524"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q6LX38"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="26853941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="10508614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="4XCG"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="4XCI"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="4XCI"/>
        </source>
    </evidence>
    <sequence length="559" mass="59675" checksum="46E6778E3A81F34C" modified="2001-06-20" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2026-01-28" modified="2026-01-28" version="1" xmlns="https://uniprot.org/uniprot">
    <accession>P0DY89</accession>
    <name>RS33_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein aS33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps33a</name>
        <name evidence="4" type="ordered locus">SSO6778.1</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558).</text>
    </comment>
    <comment type="function">
        <text evidence="2">Located near the mRNA exit channel, it binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S small ribosomal subunit. Interacts with uS7, uS9 and eS28 (PubMed:39753558).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ribosomal protein aS33 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="d=1-72"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="d=1-72"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="d=1-72"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="d=1-72"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="d=1-72"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="d=1-72"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="d=1-72"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="d=1-72"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00873">
        <property type="entry name" value="Ribosomal_aS33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000463550" description="Small ribosomal subunit protein aS33">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AE006641"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="72" mass="8384" checksum="65F01345695955DC" modified="2026-01-28" version="1">MEMLKIKLSSGREVEINDDTIAVLNEYVRTQMTLEELSKRLGLSGWEEAYELIKQVPAWVMWSPLPIYKKLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1994-02-01" modified="2026-01-28" version="141" xmlns="https://uniprot.org/uniprot">
    <accession>P35026</accession>
    <name>RS7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein uS7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 7">30S ribosomal protein S7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps7</name>
        <name evidence="6" type="synonym">rps7AB</name>
        <name type="ordered locus">SSO0217</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1994" name="Biochem. Mol. Biol. Int." volume="33" first="927" last="937">
            <title>Organization of a Sulfolobus solfataricus gene cluster homologous to the Escherichia coli str operon.</title>
            <authorList>
                <person name="Ianniciello G."/>
                <person name="Gallo M."/>
                <person name="Arcari P."/>
                <person name="Bocchini V."/>
            </authorList>
            <dbReference type="PubMed" id="7527264"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1993" name="Nucleic Acids Res." volume="21" first="1666" last="1666">
            <title>Primary structure of the elongation factor 1 alpha in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Arcari P."/>
                <person name="Gallo M."/>
                <person name="Ianniciello G."/>
                <person name="Dello Russo A."/>
                <person name="Bocchini V."/>
            </authorList>
            <dbReference type="PubMed" id="8369039"/>
            <dbReference type="DOI" id="10.1093/nar/21.7.1666"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 100-193</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference evidence="8 9 10" key="4">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Part of the 30S small ribosomal subunit, interacts with aS33.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="X76767">
        <property type="protein sequence ID" value="CAA54161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X70701">
        <property type="protein sequence ID" value="CAA50032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90163">
        <property type="entry name" value="A90163"/>
    </dbReference>
    <dbReference type="PIR" id="S33718">
        <property type="entry name" value="S33718"/>
    </dbReference>
    <dbReference type="PIR" id="T11746">
        <property type="entry name" value="T11746"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990466.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="H=1-193"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="H=1-193"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="H=1-193"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="H=1-193"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="H=1-193"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="H=1-193"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="H=1-193"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="H=1-193"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="P35026"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="P35026"/>
    <dbReference type="FunCoup" id="P35026">
        <property type="interactions" value="260"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0217"/>
    <dbReference type="PaxDb" id="273057-SSO0217"/>
    <dbReference type="EnsemblBacteria" id="AAK40560">
        <property type="protein sequence ID" value="AAK40560"/>
        <property type="gene ID" value="SSO0217"/>
    </dbReference>
    <dbReference type="GeneID" id="1455370"/>
    <dbReference type="KEGG" id="sso:SSO0217"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.215"/>
    <dbReference type="eggNOG" id="arCOG04254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063975_0_0_2"/>
    <dbReference type="InParanoid" id="P35026"/>
    <dbReference type="PhylomeDB" id="P35026"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="30S ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal protein S7 domain"/>
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        <property type="entry name" value="Ribosomal_uS7_A"/>
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        <property type="entry name" value="Ribosomal_uS7"/>
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        <property type="entry name" value="Ribosomal_uS7_dom"/>
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        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
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        <property type="entry name" value="PRK04027.1"/>
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        <property type="entry name" value="uS7_euk_arch"/>
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    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
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    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
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    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
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    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
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    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000124413" description="Small ribosomal subunit protein uS7">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: MT-4.">
        <original>G</original>
        <variation>S</variation>
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: MT-4.">
        <original>S</original>
        <variation>A</variation>
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: MT-4.">
        <original>A</original>
        <variation>S</variation>
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="sequence variant" description="In strain: MT-4.">
        <original>S</original>
        <variation>P</variation>
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="7527264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="7">
        <source>
            <dbReference type="EMBL" id="CAA50032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="10">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="193" mass="21998" checksum="C0BEC556EF96B764" modified="2001-06-20" version="3">MSLENLQLDIKVFGKWDTKVEIRDPSLKKYISLMPVYLPHTGGRHEHRRFGKAKVPIVERLINQIMRPGRNKGKKHLAYNIVKLAFDIIYLKTGQNPIQVLVRAIENSAPREEVTRIMYGGIVYYVAVDVSPQRRIDLALRHIATGAKDASFNNPKPIEEVLAEEIIAAANNDSKSFAIKRKEEIERIALSSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2025-06-18" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>P50382</accession>
    <name>TRPA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tryptophan synthase alpha chain</fullName>
            <ecNumber evidence="1">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpA</name>
        <name type="ordered locus">SSO0889</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="1995-07" db="EMBL/GenBank/DDBJ databases">
            <title>The tryptophan operon in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Tutino M.L."/>
                <person name="Cubellis M."/>
                <person name="Sannia G."/>
                <person name="Marino G."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpA family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAA90308" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="1"/>
    <dbReference type="EMBL" id="Z50014">
        <property type="protein sequence ID" value="CAA90308.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90239">
        <property type="entry name" value="E90239"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992303.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="5N2P">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.06 A"/>
        <property type="chains" value="A=4-244"/>
    </dbReference>
    <dbReference type="PDB" id="6HUL">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.55 A"/>
        <property type="chains" value="A=4-244"/>
    </dbReference>
    <dbReference type="PDBsum" id="5N2P"/>
    <dbReference type="PDBsum" id="6HUL"/>
    <dbReference type="AlphaFoldDB" id="P50382"/>
    <dbReference type="SMR" id="P50382"/>
    <dbReference type="FunCoup" id="P50382">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0889"/>
    <dbReference type="PaxDb" id="273057-SSO0889"/>
    <dbReference type="EnsemblBacteria" id="AAK41172">
        <property type="protein sequence ID" value="AAK41172"/>
        <property type="gene ID" value="SSO0889"/>
    </dbReference>
    <dbReference type="GeneID" id="44129820"/>
    <dbReference type="KEGG" id="sso:SSO0889"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.893"/>
    <dbReference type="eggNOG" id="arCOG01086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_0_2_2"/>
    <dbReference type="InParanoid" id="P50382"/>
    <dbReference type="PhylomeDB" id="P50382"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009621">
        <property type="entry name" value="PRK13125.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406:SF1">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE ALPHA CHAIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE, ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000098900" description="Tryptophan synthase alpha chain">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="6"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="35"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="50"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="59"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="79"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="89"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="106"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="112"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="117"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="120"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="129"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="144"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="158"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="174"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="189"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="198"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="210"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="227"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="5N2P"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="6HUL"/>
        </source>
    </evidence>
    <sequence length="245" mass="27387" checksum="AD73236D7CFAC70F" modified="2001-06-20" version="2">MEMGKMLVVYMTLGYPNVQSFKDFIIGAVENGADILELGIPPKYAKYDGPVIRKSYDKVKGLDIWPLIEDIRKDVGVPIIALTYLEDWVDQLENFLNMIKDVKLDGILFPDLLIDYIDDLDKIDGIIKNKGLKNVIFTSPSVPDLLIHKVSKISDLFLYYGVRPTTGVPIPVSVKQLINRVRNLVENKLIVGFGLSSESDLRDALSAGADGIAIGTVFIEEIERNGVKSAINLVKKFRAILDEYK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-20" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>P58176</accession>
    <name>SYL1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Leucine--tRNA ligase 1</fullName>
            <ecNumber evidence="1">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Leucyl-tRNA synthetase 1</fullName>
            <shortName evidence="1">LeuRS 1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuS1</name>
        <name type="ordered locus">SSO0504</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90195">
        <property type="entry name" value="H90195"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991397.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58176"/>
    <dbReference type="SMR" id="P58176"/>
    <dbReference type="FunCoup" id="P58176">
        <property type="interactions" value="281"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0504"/>
    <dbReference type="PaxDb" id="273057-SSO0504"/>
    <dbReference type="EnsemblBacteria" id="AAK40823">
        <property type="protein sequence ID" value="AAK40823"/>
        <property type="gene ID" value="SSO0504"/>
    </dbReference>
    <dbReference type="GeneID" id="1455633"/>
    <dbReference type="KEGG" id="sso:SSO0504"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.498"/>
    <dbReference type="eggNOG" id="arCOG00809">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004174_0_0_2"/>
    <dbReference type="InParanoid" id="P58176"/>
    <dbReference type="PhylomeDB" id="P58176"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07959">
        <property type="entry name" value="Anticodon_Ia_Leu_AEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000045">
        <property type="entry name" value="Leucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.20">
        <property type="entry name" value="Class I aminoacyl-tRNA synthetases (RS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.720">
        <property type="entry name" value="leucyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_A">
        <property type="entry name" value="Leu_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020791">
        <property type="entry name" value="Leu-tRNA-lgase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008957">
        <property type="entry name" value="PRK12300.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152144" description="Leucine--tRNA ligase 1">
        <location>
            <begin position="1"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="616"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="619"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <sequence length="934" mass="108527" checksum="350A9D7C8424DBD0" modified="2001-06-20" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1992-03-01" modified="2026-01-28" version="138" xmlns="https://uniprot.org/uniprot">
    <accession>P80053</accession>
    <name>DHE2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Glutamate dehydrogenase 2</fullName>
            <shortName>GDH-2</shortName>
            <ecNumber>1.4.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">gdhA-2</name>
        <name type="synonym">gdhA</name>
        <name type="ordered locus">SSO1907</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1992" name="Eur. J. Biochem." volume="203" first="81" last="87">
            <title>The protein sequence of glutamate dehydrogenase from Sulfolobus solfataricus, a thermoacidophilic archaebacterium. Is the presence of N-epsilon-methyllysine related to thermostability?</title>
            <authorList>
                <person name="Maras B."/>
                <person name="Consalvi V."/>
                <person name="Chiaraluce R."/>
                <person name="Politi L."/>
                <person name="de Rosa M."/>
                <person name="Bossa F."/>
                <person name="Scandurra R."/>
                <person name="Barra D."/>
            </authorList>
            <dbReference type="PubMed" id="1730244"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1992.tb19831.x"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-420</scope>
        <scope>ACETYLATION AT MET-2</scope>
        <scope>METHYLATION AT LYS-254; LYS-260; LYS-372; LYS-391 AND LYS-392</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamate + NAD(+) + H2O = 2-oxoglutarate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamate + NADP(+) + H2O = 2-oxoglutarate + NH4(+) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text>Homohexamer.</text>
    </comment>
    <comment type="PTM">
        <text>Methylation of lysine residues may play a role in the thermal stability of this enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90355">
        <property type="entry name" value="D90355"/>
    </dbReference>
    <dbReference type="PIR" id="S20286">
        <property type="entry name" value="S20286"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P80053"/>
    <dbReference type="SMR" id="P80053"/>
    <dbReference type="FunCoup" id="P80053">
        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1907"/>
    <dbReference type="iPTMnet" id="P80053"/>
    <dbReference type="PaxDb" id="273057-SSO1907"/>
    <dbReference type="EnsemblBacteria" id="AAK42099">
        <property type="protein sequence ID" value="AAK42099"/>
        <property type="gene ID" value="SSO1907"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1907"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1968"/>
    <dbReference type="eggNOG" id="arCOG01352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_1_2_2"/>
    <dbReference type="InParanoid" id="P80053"/>
    <dbReference type="BRENDA" id="1.4.1.3">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="P80053"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004354">
        <property type="term" value="F:glutamate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0007">Acetylation</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000182761" description="Glutamate dehydrogenase 2">
        <location>
            <begin position="2"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="modified residue" description="N-acetylmethionine" evidence="2">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine" evidence="2">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine" evidence="2">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine" evidence="2">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine" evidence="2">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-methyllysine" evidence="2">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AA sequence." evidence="3" ref="2">
        <original>E</original>
        <variation>EE</variation>
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AA sequence." evidence="3" ref="2">
        <original>K</original>
        <variation>KK</variation>
        <location>
            <position position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="1730244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="420" mass="46031" checksum="D9283C66D305EED5" modified="2008-03-18" version="3">MMEEVLSSSLYTQQVKKLYKVGELLGLDNETLETLSQPERIIQVKIQIRGSDGKLKTFMGWRSQHNSALGPYKGGVRYHPNVTQDEVEALSMIMTWKNSLLLLPYGGGKGGVRVDPKKLTREELEQLSRKYIQAIYKYLGSELDIPAPDVNTDSQTMAWFLDEYIKITGKVDFAVFTGKPVELGGIGVRLYSTGLGVATIAKEAANKFIGGVEEARVIIQGFGNVGYYAGKFLSEMGAKIVGVSDSKGGVINEKGIDVGKAIEIKEKTGSVINYPEGRKVTNEELLISDCDILIPAALENVINKFNAPKVKAKLIVEGANGPLTADADEIMRQRGIAVVPDILANAGGVVGSYVEWANNKMGEIISDEEAKKLIVDRMNNAFNTLYDYHQKKLEDHDLRTAAMALAVDRVVRAMKARGIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>P95990</accession>
    <name>RL18E_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L18e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl18e</name>
        <name type="ordered locus">SSO0070</name>
        <name type="ORF">C05001</name>
        <name type="ORF">C05_040</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75418">
        <property type="entry name" value="S75418"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988880.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95990"/>
    <dbReference type="SMR" id="P95990"/>
    <dbReference type="FunCoup" id="P95990">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0070"/>
    <dbReference type="PaxDb" id="273057-SSO0070"/>
    <dbReference type="EnsemblBacteria" id="AAK40432">
        <property type="protein sequence ID" value="AAK40432"/>
        <property type="gene ID" value="SSO0070"/>
    </dbReference>
    <dbReference type="GeneID" id="1455323"/>
    <dbReference type="KEGG" id="sso:SSO0070"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.71"/>
    <dbReference type="eggNOG" id="arCOG00780">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146465_0_0_2"/>
    <dbReference type="InParanoid" id="P95990"/>
    <dbReference type="PhylomeDB" id="P95990"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00329">
        <property type="entry name" value="Ribosomal_eL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021132">
        <property type="entry name" value="Ribosomal_eL18/eL18-A/B/_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022947">
        <property type="entry name" value="Ribosomal_eL18_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_uL15_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003079">
        <property type="entry name" value="PRK04005.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01106">
        <property type="entry name" value="RIBOSOMAL_L18E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000132803" description="Large ribosomal subunit protein eL18">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="119" mass="13492" checksum="0FA9A26B198AEE2E" modified="1997-05-01" version="1">MKVTGSTNITVRKLIRNLEKSKKPLWRKVAEELSIPSRKRPYINLYKINEHTKPNDIVVVPGKVLGIGKLDHEVTVIALDFSKSAIEKIRASGGQAMSIYKALETFKDFKGRSVRLMKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>P96039</accession>
    <name>RL10_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Acidic ribosomal protein P0 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>L10e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl10</name>
        <name evidence="1" type="synonym">rplP0</name>
        <name type="ordered locus">SSO0344</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="Biochim. Biophys. Acta" volume="1340" first="170" last="177">
            <title>Nucleotide sequence of a gene cluster encoding NusG and the L11-L1-L10-L12 ribosomal proteins from the thermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Geiger M."/>
                <person name="Groebner P."/>
                <person name="Piendl W."/>
            </authorList>
            <dbReference type="PubMed" id="9252104"/>
            <dbReference type="DOI" id="10.1016/S0167-4838(97)00073-3"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="miscellaneous">
        <text>Was called L10e in this organism; in this case 'e' is for E.coli-like, not eukaryotic-type protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99526" version="1"/>
        </conflict>
        <text>Truncated N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="U85262">
        <property type="protein sequence ID" value="AAB99526.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90177">
        <property type="entry name" value="B90177"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990632.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P96039"/>
    <dbReference type="SMR" id="P96039"/>
    <dbReference type="FunCoup" id="P96039">
        <property type="interactions" value="218"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0344"/>
    <dbReference type="PaxDb" id="273057-SSO0344"/>
    <dbReference type="EnsemblBacteria" id="AAK40673">
        <property type="protein sequence ID" value="AAK40673"/>
        <property type="gene ID" value="SSO0344"/>
    </dbReference>
    <dbReference type="GeneID" id="1455483"/>
    <dbReference type="KEGG" id="sso:SSO0344"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.334"/>
    <dbReference type="eggNOG" id="arCOG04288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053173_0_0_2"/>
    <dbReference type="InParanoid" id="P96039"/>
    <dbReference type="PhylomeDB" id="P96039"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00280">
        <property type="entry name" value="Ribosomal_uL10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022909">
        <property type="entry name" value="Ribosomal_uL10_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003095">
        <property type="entry name" value="PRK04019.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000154807" description="Large ribosomal subunit protein uL10">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="298"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="309"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="338" mass="37061" checksum="BBA6452BD72592DD" modified="2001-06-20" version="2">MKRLALALKQRKVASWKLEEVKELTELIKNSNTILIGNLEGFPADKLHEIRKKLRGKATIKVTKNTLFKIAAKNAGIDIEKLEQYLTGPNVFIFTKDNPFITNMFFENYKLRRYAMPGDKAEEEVVIPAGDTGMPAGPILSVFGKLKVQTKVQDGKVHVVKDTVVAKPGDVIPAEALPILQKLGIMPVYVKLKIKVAYHEGLVIPAESLKLDLEGYRSNITEAYRNAFTLAVEIAYPTPDVLKFTISKVFKNAIALASEIGYITPESAQAVISKAVAKAYALATAIGGKVDLGVQLPTVQQSQSQQPAAEEKKEEKKEEEKKGPSEEEIASGLASLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX64</accession>
    <name>Q7LX64_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter, ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO2137</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ABC transporter superfamily. Drug exporter-1 (DrugE1) (TC 3.A.1.105) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74048">
        <property type="entry name" value="S74048"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX64"/>
    <dbReference type="FunCoup" id="Q7LX64">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2137"/>
    <dbReference type="PaxDb" id="273057-SSO2137"/>
    <dbReference type="EnsemblBacteria" id="AAK42315">
        <property type="protein sequence ID" value="AAK42315"/>
        <property type="gene ID" value="SSO2137"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2137"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2228"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q7LX64"/>
    <dbReference type="PhylomeDB" id="Q7LX64"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043215">
        <property type="term" value="P:daunorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900753">
        <property type="term" value="P:doxorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000589">
        <property type="entry name" value="ABC transporter, ATP-binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005894">
        <property type="entry name" value="DrrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DrrA1/2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01188">
        <property type="entry name" value="drrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43582">
        <property type="entry name" value="LINEARMYCIN RESISTANCE ATP-BINDING PROTEIN LNRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43582:SF2">
        <property type="entry name" value="LINEARMYCIN RESISTANCE ATP-BINDING PROTEIN LNRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DrrA1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="12"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="339" mass="38225" checksum="2376649968765361" modified="2011-05-31" version="1">MRRLLKMRNVAIKAINLTKRFGKFVAVDHINFEVYEGEIFGFLGPNGAGKSTTIKMLTTVLKPSEGTATVSGYDVIKQPALVRQSIGVVPQEYTADEDLTGWENMMMMAGLYGIPKRIAEERAKELLEMVELTYAAKRKVETYSGGMRRRLEIAMSLISRPRILFLDEPTLGLDAQTRAAIWQYIMKLKEEYKMTIFVTTHYLEEADMYGDRIAIIDKGKILAIGSPKELKEKVGGDVVSLQTSNDELAIKIISSSKDGILDVRKASDGIRIKVRNGEEKAPEILESLVKNGIKVSRMSITEPTMDEVYMEFTGKSLRDEEASAQEMFAFRRTMRRART</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXE9</accession>
    <name>Q7LXE9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase FkbM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E99233">
        <property type="entry name" value="E99233"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXE9"/>
    <dbReference type="SMR" id="Q7LXE9"/>
    <dbReference type="STRING" id="273057.SSO0829"/>
    <dbReference type="PaxDb" id="273057-SSO0829"/>
    <dbReference type="EnsemblBacteria" id="AAK41124">
        <property type="protein sequence ID" value="AAK41124"/>
        <property type="gene ID" value="SSO0829"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0829"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.843"/>
    <dbReference type="eggNOG" id="arCOG01400">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079272_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXE9"/>
    <dbReference type="PhylomeDB" id="Q7LXE9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052514">
        <property type="entry name" value="SAM-dependent_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203">
        <property type="entry name" value="METHYLTRANSFERASE, FKBM FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203:SF15">
        <property type="entry name" value="SLL1173 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase FkbM" evidence="1">
        <location>
            <begin position="120"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="301" mass="34223" checksum="FDA77576BED21A59" modified="2011-05-31" version="1">MGVFSKLRKSVGFTRLLLRYVDNWFDILYLTIANKKLAFLEVKLKDNNRIFLKNSIYDLYALLSLLKKGWKVKEADENNAILCKEVCLKIRTKKGADIGHIDEIYECKTYGESFKGVVIDVGASNADSSIFFATNGAEKVIALEPFPESYELGKYNIKINNLDNKVILLPYALTNYEGYVDFIISSDNPNVNTVSPSTQVMGLLGIKFDQKIKVPTTTLASLIQKYNIDRVYLLKMDCEGCEYAILRDLPAEVLNKIENIILEFHDYPQDLPDILRKAGFNVNYENKPIGILKAYKNDKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXG2</accession>
    <name>Q7LXG2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase FkbM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0814</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90231">
        <property type="entry name" value="H90231"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923106.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXG2"/>
    <dbReference type="STRING" id="273057.SSO0814"/>
    <dbReference type="PaxDb" id="273057-SSO0814"/>
    <dbReference type="EnsemblBacteria" id="AAK41111">
        <property type="protein sequence ID" value="AAK41111"/>
        <property type="gene ID" value="SSO0814"/>
    </dbReference>
    <dbReference type="GeneID" id="1455078"/>
    <dbReference type="KEGG" id="sso:SSO0814"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.828"/>
    <dbReference type="eggNOG" id="arCOG01401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1076134_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXG2"/>
    <dbReference type="PhylomeDB" id="Q7LXG2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052514">
        <property type="entry name" value="SAM-dependent_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203">
        <property type="entry name" value="METHYLTRANSFERASE, FKBM FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203:SF15">
        <property type="entry name" value="SLL1173 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase FkbM" evidence="1">
        <location>
            <begin position="50"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="258" mass="29230" checksum="B1E1F71CEC98DEFB" modified="2011-05-31" version="1">MMVDLRPFTGIPAKAILSRRSDFAAFYQVIIENIYSHLLSSIREGDVVIDAGANIGLFSILASFKVKEKGRVIAIEPEPNNLKILKQNVELNGLNNVIIIPKALYDKPGKRVNMRGEGVGAFVSEDGEGTVETTTLDVIAEENRLRPRILKMDIEGAEGKALLGSINTVKYLELIEMEVHDEENMKQVEEILFRFKFKKKEVAIEDLRNVYRQFIRHPINILRIEAANRFSTTKRVLLSRINGGKVSLFPKQIVYYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXN1</accession>
    <name>Q7LXN1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-ethylammeline chlorohydrolase, putative (TrzA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">trzA</name>
        <name evidence="3" type="ordered locus">SSO0679</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90215">
        <property type="entry name" value="F90215"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991227.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXN1"/>
    <dbReference type="FunCoup" id="Q7LXN1">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0679"/>
    <dbReference type="PaxDb" id="273057-SSO0679"/>
    <dbReference type="EnsemblBacteria" id="AAK40981">
        <property type="protein sequence ID" value="AAK40981"/>
        <property type="gene ID" value="SSO0679"/>
    </dbReference>
    <dbReference type="GeneID" id="1454948"/>
    <dbReference type="KEGG" id="sso:SSO0679"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.680"/>
    <dbReference type="eggNOG" id="arCOG00695">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_2_1_2"/>
    <dbReference type="InParanoid" id="Q7LXN1"/>
    <dbReference type="PhylomeDB" id="Q7LXN1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01298">
        <property type="entry name" value="ATZ_TRZ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050287">
        <property type="entry name" value="MTA/SAH_deaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794:SF11">
        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794">
        <property type="entry name" value="AMINOHYDROLASE SSNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="2">
        <location>
            <begin position="53"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="411" mass="47536" checksum="9F521A17E0F58CDC" modified="2011-05-31" version="1">MQIRNNGYTLKNCRFIVNYDKILENVNIVIQNGYIKNVGREVEGDEVDCSEYIAIPGLVNAHTHTPMIILRGYYDDAELTEWLKKIWEFEKVFKLNEMNIASELAIMEMLSKGTTAFIDMYFNPEGVKEIAEKYGIRAYAGYTFLNSLFEPHEIDKKQRQLKTSELFKPIVNVHSIYSTSIETLKLAKQIAEETDTWIHIHVSETRSEIYEIKKKYGKFPVELLNELGIVKNAQLVHLGWIANWELRYVNQATHCPTSNMKLATAGFFPFKELMENGVNITIGTDGAASNNSLDMFREMKNAVLLQRHSYWDVGVKAFHAFRAATENGYKLINLKGGRIEEGYIADIVLLKKDKLYPLRKDRILSNIVYYTVGEYVGKVIVNGRIVYDEKVEREFDKRRRELLKLLDEIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-02-05" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXX5</accession>
    <name>Q7LXX5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Third ORF in transposon ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90197">
        <property type="entry name" value="B90197"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXX5"/>
    <dbReference type="PaxDb" id="273057-SSO0514"/>
    <dbReference type="EnsemblBacteria" id="AAK40833">
        <property type="protein sequence ID" value="AAK40833"/>
        <property type="gene ID" value="SSO0514"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0514"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.509"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036902_12_2_2"/>
    <dbReference type="InParanoid" id="Q7LXX5"/>
    <dbReference type="PhylomeDB" id="Q7LXX5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="1">
        <location>
            <begin position="16"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="137" mass="15931" checksum="8824272C457770AB" modified="2011-05-31" version="1">MKEVRKMIEGNVPQDHVLLTIPGIGKLAAGIIIGIVGDIRRFPRPESFVAYCGLDPVVERSGRIEVRKGISKRGNKYLRSLFYFLAETQYSRNPTLLKFYEAHKDKLKGRKLYTALARKLPKYRWSVWYNNKPYEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-02-05" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LY00</accession>
    <name>Q7LY00_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AIR synthase related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0076</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75412">
        <property type="entry name" value="S75412"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LY00"/>
    <dbReference type="STRING" id="273057.SSO0076"/>
    <dbReference type="PaxDb" id="273057-SSO0076"/>
    <dbReference type="EnsemblBacteria" id="AAK40437">
        <property type="protein sequence ID" value="AAK40437"/>
        <property type="gene ID" value="SSO0076"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0076"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.76"/>
    <dbReference type="eggNOG" id="arCOG00637">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_648312_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LY00"/>
    <dbReference type="PhylomeDB" id="Q7LY00"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02691">
        <property type="entry name" value="PurM-like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009186">
        <property type="entry name" value="Ni_metllenz_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270:SF2">
        <property type="entry name" value="HYDROGENASE EXPRESSION_FORMATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="THIAMINE-MONOPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006346">
        <property type="entry name" value="Ni_metllenz_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="1">
        <location>
            <begin position="103"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="2">
        <location>
            <begin position="224"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="48226" checksum="B3E93332781B30ED" modified="2011-05-31" version="1">MALYFALMDLEGIVRRLYPDIEKAKTKLIEEIRFYKSDRYNAEEISNVILTEVINSMKADDKFGFPKTNIRAGEAGLGSRGIGDNLIHTKLFELAGKQIEEYDDAGIRENVVVSIDGIHSRLSYFPFLAGFYATRATLRDIMVKGAYPLGLIVDIHLSDDSDIGMLIDFEAGVTTISKALDIPILAGSTLRIGGDLVLGDRISGAVGSIGILKSKDFLRKRVKKGLKILMTEGNGGGTIATTAIYNGMPDVISETLNIKDLLTCIIVRDYLSSDVYSMTDVTNGGIRADALEVSKITNLSFVIDEEKFLSLINPKVRKMLNEINIDPFGISIDSILIFTDNPDLVKKRLAQYNIRSEIVGYIDDFRQYPIITYDGKELKPQFRESPYTPIKKYFGNYSPYSIEYISNRLDYAIAEAKTKMDNVLKNLKTSFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2024-07-24" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LY08</accession>
    <name>Q7LY08_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transmembrane type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO0051</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75437">
        <property type="entry name" value="S75437"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LY08"/>
    <dbReference type="STRING" id="273057.SSO0051"/>
    <dbReference type="PaxDb" id="273057-SSO0051"/>
    <dbReference type="EnsemblBacteria" id="AAK40416">
        <property type="protein sequence ID" value="AAK40416"/>
        <property type="gene ID" value="SSO0051"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0051"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.54"/>
    <dbReference type="eggNOG" id="arCOG01463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_843639_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LY08"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052902">
        <property type="entry name" value="ABC-2_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43027">
        <property type="entry name" value="DOXORUBICIN RESISTANCE ABC TRANSPORTER PERMEASE PROTEIN DRRC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43027:SF1">
        <property type="entry name" value="DOXORUBICIN RESISTANCE ABC TRANSPORTER PERMEASE PROTEIN DRRC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="6">
        <location>
            <begin position="110"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="336" mass="37849" checksum="745CA9BA667513CE" modified="2011-05-31" version="1">MRTMNRILLTVSALVKDNLRNRFVIFWIIVFPIIITLLFGLVFGGFSSYFHVTVAVEGNYGLAKYLNSTEIFQGVTNVSLKYAAEHDYIYVISNGSSFTIYTSKQNQEFVPVLESVIEQYYQKVNNTYDFTVLQISNYTYYNYLISGIIGIISLSNGILSTIGVSASYFRDRIIERLATSPLKSYEWVISLIIYVIIITLISTTAVIILGILFGFIPIIGIEFLGFLVIATLLFGGLGAIIYGLTPKDKIFLSEIVANVLVFPLMFLSNAFFPPNVYPPIVRIFVEYQPLSVIITIIRDLIVYGISPNPILVSIVFLFTIVFLYAGGRLLRLREIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TW6</accession>
    <name>Q97TW6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1160</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="3">Third ORF in transposon ISC1160</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO7627</name>
        <name evidence="3" type="ordered locus">SSO7754</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2001-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK67517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK67518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97TW6"/>
    <dbReference type="PaxDb" id="273057-SSO7627"/>
    <dbReference type="EnsemblBacteria" id="AAK67517">
        <property type="protein sequence ID" value="AAK67517"/>
        <property type="gene ID" value="SSO7627"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAK67518">
        <property type="protein sequence ID" value="AAK67518"/>
        <property type="gene ID" value="SSO7754"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7627"/>
    <dbReference type="KEGG" id="sso:SSO7754"/>
    <dbReference type="eggNOG" id="arCOG07929">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192666_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK67517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK67518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="63" mass="7709" checksum="26A42AE492D94FE6" modified="2001-10-01" version="1">MVFRLYNKVNPIETSYRNIKSFLPFISSTRLAFRKLMFMIALYFLLTIFKDVKREDFYSSFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97TY9</accession>
    <name>Q97TY9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Winged helix-turn-helix transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="UCP032840"/>
    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="HTH_24"/>
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    </dbReference>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="70"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="12359" checksum="2714E43186E5D757" modified="2001-10-01" version="1">MELTPRLQDIINMLKNKGTMNVKDLALEIKVSPKTAKGYARELQRLGLVEMDQDGNIRLREYEEEAHIDSMKLLKTLENHENEITLLKKEIDTIKGELEKLKKKGKA</sequence>
</entry>
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    <accession>Q97U20</accession>
    <name>Q97U20_SACS2</name>
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        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO3205</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2008-06" db="PDB data bank">
            <title>Crystal structure of CBS domain-containing protein in complex with AMP (NP_344512.1) from SULFOLOBUS SOLFATARICUS at 1.80 A resolution.</title>
            <authorList>
                <consortium name="Joint Center for Structural Genomics (JCSG)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH AMP</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90505">
        <property type="entry name" value="G90505"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991674.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="3DDJ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A=1-277"/>
    </dbReference>
    <dbReference type="PDBsum" id="3DDJ"/>
    <dbReference type="AlphaFoldDB" id="Q97U20"/>
    <dbReference type="SMR" id="Q97U20"/>
    <dbReference type="FunCoup" id="Q97U20">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3205"/>
    <dbReference type="PaxDb" id="273057-SSO3205"/>
    <dbReference type="EnsemblBacteria" id="AAK43302">
        <property type="protein sequence ID" value="AAK43302"/>
        <property type="gene ID" value="SSO3205"/>
    </dbReference>
    <dbReference type="GeneID" id="1453221"/>
    <dbReference type="KEGG" id="sso:SSO3205"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3307"/>
    <dbReference type="eggNOG" id="arCOG00600">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_999709_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U20"/>
    <dbReference type="PhylomeDB" id="Q97U20"/>
    <dbReference type="EvolutionaryTrace" id="Q97U20"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17777">
        <property type="entry name" value="CBS_arch_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17778">
        <property type="entry name" value="CBS_arch_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="6" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="7"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="80"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="143"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="214"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
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        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
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        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>AMP</name>
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        </ligand>
    </feature>
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        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="3DDJ"/>
        </source>
    </evidence>
    <sequence length="277" mass="31258" checksum="34068F7A1F0363D6" modified="2001-10-01" version="1">MNIETLMIKNPPILSKEDRLGSAFKKINEGGIGRIIVANEKIEGLLTTRDLLSTVESYCKDSCSQGDLYHISTTPIIDYMTPNPVTVYNTSDEFTAINIMVTRNFGSLPVVDINDKPVGIVTEREFLLLYKDLDEIFPVKVFMSTKVQTIYKEVRLDQAVKLMLRRGFRRLPVIDDDNKVVGIVTVVNAIKQLAKAVDKLDPDYFYGKVVKDVMVTNLVTIDELASVNRAAAEMIVKRIGSLLILNKDNTIRGIITERDLLIALHHILVMEKFKEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U50</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO3174</name>
    </gene>
    <organism evidence="4 5">
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        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
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            <taxon>Thermoproteota</taxon>
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    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="A99502"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    </dbReference>
    <dbReference type="STRING" id="273057.SSO3174"/>
    <dbReference type="PaxDb" id="273057-SSO3174"/>
    <dbReference type="EnsemblBacteria" id="AAK43272">
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        <property type="gene ID" value="SSO3174"/>
    </dbReference>
    <dbReference type="GeneID" id="1453191"/>
    <dbReference type="KEGG" id="sso:SSO3174"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3278"/>
    <dbReference type="eggNOG" id="arCOG00606">
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    <dbReference type="HOGENOM" id="CLU_040681_12_1_2"/>
    <dbReference type="InParanoid" id="Q97U50"/>
    <dbReference type="PhylomeDB" id="Q97U50"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09836">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00571">
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    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="7"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="71"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="131" mass="14286" checksum="8E9ADD843D6C8134" modified="2001-10-01" version="1">MYVADLITRNPLTVKPETSIRDAAKIMKRENLGSLIIVDETNKPIGIVTERDILRAVADEVALDSPVSTIMTRGLITIPPNKDVTEALIIMYQNNVRHLAVVGQSGELVGVISIRDAAKAVNLLALDLSIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UC4</accession>
    <name>Q97UC4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TQO small subunit DoxD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO3091</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90492">
        <property type="entry name" value="F90492"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924130.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UC4"/>
    <dbReference type="STRING" id="273057.SSO3091"/>
    <dbReference type="PaxDb" id="273057-SSO3091"/>
    <dbReference type="EnsemblBacteria" id="AAK43197">
        <property type="protein sequence ID" value="AAK43197"/>
        <property type="gene ID" value="SSO3091"/>
    </dbReference>
    <dbReference type="GeneID" id="1453116"/>
    <dbReference type="KEGG" id="sso:SSO3091"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3199"/>
    <dbReference type="eggNOG" id="arCOG02863">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507450_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UC4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007301">
        <property type="entry name" value="DoxD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39157:SF1">
        <property type="entry name" value="DOXX FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39157">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04173">
        <property type="entry name" value="DoxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="TQO small subunit DoxD" evidence="2">
        <location>
            <begin position="10"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="178" mass="19769" checksum="DB9C8E429245EACC" modified="2001-10-01" version="1">MAKTMISDNFTLILRISMASIWLYAGILGKLLNSGFLNPSSEQYVGFTIQYLAQGSIIRQFLYIVAMPHPVLVGTLVMIGEISFGILTLIGLMSRLCSVVAFYTNLIYFLSASWTGAEEYGLNLLMMIINAYIIAYGSGNFSIDSLVLRKFNVVNNKILWIIVGSVIYIAVIVYLILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UH5</accession>
    <name>Q97UH5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter, binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO3043</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99486">
        <property type="entry name" value="A99486"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924107.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UH5"/>
    <dbReference type="FunCoup" id="Q97UH5">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3043"/>
    <dbReference type="PaxDb" id="273057-SSO3043"/>
    <dbReference type="EnsemblBacteria" id="AAK43144">
        <property type="protein sequence ID" value="AAK43144"/>
        <property type="gene ID" value="SSO3043"/>
    </dbReference>
    <dbReference type="GeneID" id="1453063"/>
    <dbReference type="KEGG" id="sso:SSO3043"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3141"/>
    <dbReference type="eggNOG" id="arCOG01534">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_8_3_2"/>
    <dbReference type="InParanoid" id="Q97UH5"/>
    <dbReference type="PhylomeDB" id="Q97UH5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08509">
        <property type="entry name" value="PBP2_TmCBP_oligosaccharides_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.76.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290:SF9">
        <property type="entry name" value="OLIGOPEPTIDE-BINDING PROTEIN APPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="687"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="Solute-binding protein family 5" evidence="5">
        <location>
            <begin position="83"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK43144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="710" mass="79133" checksum="CE0641748ADE928C" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UH8</accession>
    <name>Q97UH8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta-glucosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO3039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90485">
        <property type="entry name" value="F90485"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924105.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UH8"/>
    <dbReference type="SMR" id="Q97UH8"/>
    <dbReference type="FunCoup" id="Q97UH8">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3039"/>
    <dbReference type="CAZy" id="GH116">
        <property type="family name" value="Glycoside Hydrolase Family 116"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3039"/>
    <dbReference type="EnsemblBacteria" id="AAK43141">
        <property type="protein sequence ID" value="AAK43141"/>
        <property type="gene ID" value="SSO3039"/>
    </dbReference>
    <dbReference type="GeneID" id="1453060"/>
    <dbReference type="KEGG" id="sso:SSO3039"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3138"/>
    <dbReference type="eggNOG" id="arCOG03867">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027051_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UH8"/>
    <dbReference type="PhylomeDB" id="Q97UH8"/>
    <dbReference type="BRENDA" id="3.2.1.21">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="BRENDA" id="3.2.1.52">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006775">
        <property type="entry name" value="GH116_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024462">
        <property type="entry name" value="GH116_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052566">
        <property type="entry name" value="Non-lysos_glucosylceramidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654">
        <property type="entry name" value="BILE ACID BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654:SF0">
        <property type="entry name" value="NON-LYSOSOMAL GLUCOSYLCERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04685">
        <property type="entry name" value="DUF608"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12215">
        <property type="entry name" value="Glyco_hydr_116N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl-hydrolase family 116 N-terminal" evidence="2">
        <location>
            <begin position="12"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl-hydrolase family 116 catalytic region" evidence="1">
        <location>
            <begin position="333"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl-hydrolase family 116 catalytic region" evidence="1">
        <location>
            <begin position="536"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK43141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="803" mass="93418" checksum="2CAD55CDA1B009CD" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UJ4</accession>
    <name>Q97UJ4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90482">
        <property type="entry name" value="H90482"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924091.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UJ4"/>
    <dbReference type="STRING" id="273057.SSO3017"/>
    <dbReference type="PaxDb" id="273057-SSO3017"/>
    <dbReference type="EnsemblBacteria" id="AAK43119">
        <property type="protein sequence ID" value="AAK43119"/>
        <property type="gene ID" value="SSO3017"/>
    </dbReference>
    <dbReference type="GeneID" id="1453038"/>
    <dbReference type="KEGG" id="sso:SSO3017"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3110"/>
    <dbReference type="eggNOG" id="arCOG00138">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062161_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UJ4"/>
    <dbReference type="PhylomeDB" id="Q97UJ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052528">
        <property type="entry name" value="Sugar_transport-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23526">
        <property type="entry name" value="INTEGRAL MEMBRANE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23526:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="42926" checksum="19375E59F82D3698" modified="2001-10-01" version="1">MKYLKIFAILSNLANNLVSPFISFVSAYFGMNSEELALVTSATNAVPNISQYFLNFIKSKAKFLIFIGTLVNGLLWVISAFIPFNWIFLTVYFIITMSIGIANFGWNLIMDRVSRNSRGSTLSLYLVYGTIGALAATLVTGFITESNTELIREFFLISGIILVGSANLSRKIEVDTKLEENRVKSASFQMKRFLTTIFLFNLVLSLAWPIFPLAQVYKFHMNDENIAILSVETGVTTILFQRIVAKLTDTKRKLTLFLGSALYTIYPLSYALSDSIYMIYIVNLASGFTNAVGSVTYIAYIFDNSDDNTIRRNLAVYNLTVGCGVMTGSILGGVAYNYVTQFYNPIYSIDLMLILSSILRFSVSPLFLTIKDTPVVFHQFYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UN5</accession>
    <name>Q97UN5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Quinol oxidase-2, putative subunit (SoxI-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">soxI-like</name>
        <name evidence="2" type="ordered locus">SSO2968</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90477">
        <property type="entry name" value="B90477"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924079.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UN5"/>
    <dbReference type="STRING" id="273057.SSO2968"/>
    <dbReference type="PaxDb" id="273057-SSO2968"/>
    <dbReference type="EnsemblBacteria" id="AAK43073">
        <property type="protein sequence ID" value="AAK43073"/>
        <property type="gene ID" value="SSO2968"/>
    </dbReference>
    <dbReference type="GeneID" id="1452992"/>
    <dbReference type="KEGG" id="sso:SSO2968"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3059"/>
    <dbReference type="eggNOG" id="arCOG06033">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754788_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UN5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="148" mass="17188" checksum="55477E2CF9FB75B7" modified="2001-10-01" version="1">MATNRRMTSKDYALWIVTILAFFFFFLWVGDFGNLSYLTQSPITTYVETLWSVTYIAAGGVFAVFMGSIVFLSVKFRAPSEVQTIRRKVNVINYYYMALILDLLFAIIVTYEMFMMSTYQFITATLASADLLLFASIIYLVYKLYYQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UX8</accession>
    <name>Q97UX8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acetate--CoA ligase</fullName>
            <ecNumber evidence="7">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">acsA-9</name>
        <name evidence="11" type="ordered locus">SSO2863</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in the autotrophic CO(2) fixation pathway. Activates 3-hydroxypropionate to its CoA ester. Can also activate propionate, and to a lesser extent acrylate, acetate and butyrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-hydroxypropanoate + ATP + CoA = 3-hydroxypropanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:26534"/>
            <dbReference type="ChEBI" id="CHEBI:16510"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.36"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90464">
        <property type="entry name" value="C90464"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989839.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UX8"/>
    <dbReference type="FunCoup" id="Q97UX8">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2863"/>
    <dbReference type="PaxDb" id="273057-SSO2863"/>
    <dbReference type="EnsemblBacteria" id="AAK42970">
        <property type="protein sequence ID" value="AAK42970"/>
        <property type="gene ID" value="SSO2863"/>
    </dbReference>
    <dbReference type="GeneID" id="7794571"/>
    <dbReference type="KEGG" id="sso:SSO2863"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2949"/>
    <dbReference type="eggNOG" id="arCOG04201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_2"/>
    <dbReference type="InParanoid" id="Q97UX8"/>
    <dbReference type="PhylomeDB" id="Q97UX8"/>
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        <property type="term" value="F:3-hydroxypropionyl-CoA synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:acetate-CoA ligase activity"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
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        <property type="entry name" value="ACAS_N"/>
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        <property type="entry name" value="AMP-bd_C"/>
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        <property type="entry name" value="AMP-bd_C_sf"/>
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        <property type="entry name" value="AMP-binding_CS"/>
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        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
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        <property type="entry name" value="ANL_N_sf"/>
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    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF232">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="10">
        <location>
            <begin position="28"/>
            <end position="80"/>
        </location>
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    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="8">
        <location>
            <begin position="85"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="9">
        <location>
            <begin position="542"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR02188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK42970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="666" mass="75800" checksum="E2779A7118B7C2C5" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V10</accession>
    <name>Q97V10_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="29422" last="29422">
            <title>Diverse architectural properties of Sso10a proteins: Evidence for a role in chromatin compaction and organization.</title>
            <authorList>
                <person name="Driessen R.P."/>
                <person name="Lin S.N."/>
                <person name="Waterreus W.J."/>
                <person name="van der Meulen A.L."/>
                <person name="van der Valk R.A."/>
                <person name="Laurens N."/>
                <person name="Moolenaar G.F."/>
                <person name="Pannu N.S."/>
                <person name="Wuite G.J."/>
                <person name="Goosen N."/>
                <person name="Dame R.T."/>
            </authorList>
            <dbReference type="PubMed" id="27403582"/>
            <dbReference type="DOI" id="10.1038/srep29422"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 28-130</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90460">
        <property type="entry name" value="B90460"/>
    </dbReference>
    <dbReference type="PDB" id="4HW0">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B/C=28-130"/>
    </dbReference>
    <dbReference type="PDBsum" id="4HW0"/>
    <dbReference type="AlphaFoldDB" id="Q97V10"/>
    <dbReference type="SMR" id="Q97V10"/>
    <dbReference type="PaxDb" id="273057-SSO2827"/>
    <dbReference type="EnsemblBacteria" id="AAK42937">
        <property type="protein sequence ID" value="AAK42937"/>
        <property type="gene ID" value="SSO2827"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2827"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2912"/>
    <dbReference type="eggNOG" id="arCOG01055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159100_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V10"/>
    <dbReference type="PhylomeDB" id="Q97V10"/>
    <dbReference type="EvolutionaryTrace" id="Q97V10"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038723">
        <property type="entry name" value="ArnR1-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14947">
        <property type="entry name" value="HTH_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArnR1-like winged helix-turn-helix" evidence="1">
        <location>
            <begin position="33"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="47"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="4HW0"/>
        </source>
    </evidence>
    <sequence length="130" mass="15375" checksum="CE6DF93805364DF2" modified="2001-10-01" version="1">MTYGYNIFTVIKFSDNVYKLQLIYYSMQLERRKRGTMEIMFDILRNCEPKCGITRVIYGAGINYVVAQKYLDQLVKVGALNIKTENDRKIYEITEKGKLLRTHIEEFIKIRENLYSAKEKVSELLRTDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V19</accession>
    <name>Q97V19_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Electron transfer flavoprotein alpha and beta-subunit (EtfAB/fixAB)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">etfAB/fixAB</name>
        <name evidence="2" type="ordered locus">SSO2817</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90459">
        <property type="entry name" value="A90459"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989028.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V19"/>
    <dbReference type="FunCoup" id="Q97V19">
        <property type="interactions" value="133"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2817"/>
    <dbReference type="PaxDb" id="273057-SSO2817"/>
    <dbReference type="EnsemblBacteria" id="AAK42928">
        <property type="protein sequence ID" value="AAK42928"/>
        <property type="gene ID" value="SSO2817"/>
    </dbReference>
    <dbReference type="GeneID" id="1452847"/>
    <dbReference type="KEGG" id="sso:SSO2817"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2903"/>
    <dbReference type="eggNOG" id="arCOG00448">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024916_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V19"/>
    <dbReference type="PhylomeDB" id="Q97V19"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF17">
        <property type="entry name" value="PROTEIN FIXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="1">
        <location>
            <begin position="25"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="1">
        <location>
            <begin position="287"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="610" mass="67142" checksum="1A1E144BB93C8699" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V22</accession>
    <name>Q97V22_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2814</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="F90458"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989034.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V22"/>
    <dbReference type="PaxDb" id="273057-SSO2814"/>
    <dbReference type="EnsemblBacteria" id="AAK42925">
        <property type="protein sequence ID" value="AAK42925"/>
        <property type="gene ID" value="SSO2814"/>
    </dbReference>
    <dbReference type="GeneID" id="1452844"/>
    <dbReference type="KEGG" id="sso:SSO2814"/>
    <dbReference type="eggNOG" id="arCOG07722">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_189643_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V22"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="100" mass="11884" checksum="57401E9CA6C079A2" modified="2001-10-01" version="1">MLSLLSYFYILFIMNREVAVKFEDEENAFYLISDDGLIVKRNTIPKKIDILIVKQTDRSKIIEAVKMNYKLFECNNITKEECLVKVLNRVFPYCKTCRFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V45</accession>
    <name>Q97V45_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2787</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="E90455"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923969.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V45"/>
    <dbReference type="STRING" id="273057.SSO2787"/>
    <dbReference type="PaxDb" id="273057-SSO2787"/>
    <dbReference type="DNASU" id="1452819"/>
    <dbReference type="EnsemblBacteria" id="AAK42900">
        <property type="protein sequence ID" value="AAK42900"/>
        <property type="gene ID" value="SSO2787"/>
    </dbReference>
    <dbReference type="GeneID" id="1452819"/>
    <dbReference type="KEGG" id="sso:SSO2787"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2871"/>
    <dbReference type="eggNOG" id="arCOG03117">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044208_1_2_2"/>
    <dbReference type="InParanoid" id="Q97V45"/>
    <dbReference type="PhylomeDB" id="Q97V45"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051311">
        <property type="entry name" value="DedA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709">
        <property type="entry name" value="ALKALINE PHOSPHATASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709:SF6">
        <property type="entry name" value="UNDECAPRENYL PHOSPHATE TRANSPORTER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="7">
        <location>
            <begin position="27"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="194" mass="21876" checksum="0683DCD0DE9A30FC" modified="2001-10-01" version="1">MVYVFQGIGGYIILFLLMIGEGIGLPLPSEVIMPLVGYYSYLGSISLYLGVIVGTLGSLVGSIIAYIIGYKLGLAFLKRYGKYILIDEGKIEALHNWFLRYGNMAVFGFRFIPGLRALISYPAGIGQMKILKFLGFTFLGHTIWDIVLALIGYQLANQINFVITQAEKLGNYLFILVIVIIVIYILWRIFWKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V75</accession>
    <name>Q97V75_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Molybdopterin oxidoreductase, iron-sulfur binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2759</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="G90451"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V75"/>
    <dbReference type="STRING" id="273057.SSO2759"/>
    <dbReference type="PaxDb" id="273057-SSO2759"/>
    <dbReference type="EnsemblBacteria" id="AAK42870">
        <property type="protein sequence ID" value="AAK42870"/>
        <property type="gene ID" value="SSO2759"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2759"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2839"/>
    <dbReference type="eggNOG" id="arCOG01503">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_695629_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V75"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16371">
        <property type="entry name" value="DMSOR_beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050954">
        <property type="entry name" value="ET_IronSulfur_Cluster-Binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43177">
        <property type="entry name" value="PROTEIN NRFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43177:SF3">
        <property type="entry name" value="PROTEIN NRFC HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="53"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="86"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="409" mass="46418" checksum="346DE643FC96B278" modified="2001-10-01" version="1">MLLTYLTRYYHMERKLGFIFDHNKCIICNACVDACNKAYGDLNWRSLIVMTHGESKTALSIACNHCDNPTCMQVCPANAIEKNEMGIVRIRDDKCIGCGFCTWACPYEALKFNNEGVMSKCHFCYDRLAEGKGIPYCVEACPTGALAFGWIEKGEAEVNYLPPSEITKPRIVIKQPESKETLNASVLHTKKEENYLGLLAFTIGSEFALGYSLFKLPLWSIVSVILLVASLLLSVNHARKTDRFYRVIYNLKTSWLSREVLFSSIAILFYILSIAEPLFYYPAILFLTVGVISSIMIYMLKSRPSWYNVDTPVSFIGSIFTMSLPIVYFFSHNLTTIIPLIITLVIEIISNNRRSNVRMSLNVASIVISLISIVFPEIIIITEIINIVSEVTHRREFYEKVVYYGLPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VA3</accession>
    <name>Q97VA3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Agmatinase (Agmatine ureohydrolase) (SpeB-2)</fullName>
            <ecNumber evidence="6">3.5.3.11</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">speB-2</name>
        <name evidence="6" type="ordered locus">SSO2732</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="4">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.11" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90448">
        <property type="entry name" value="C90448"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991730.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VA3"/>
    <dbReference type="FunCoup" id="Q97VA3">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2732"/>
    <dbReference type="PaxDb" id="273057-SSO2732"/>
    <dbReference type="EnsemblBacteria" id="AAK42842">
        <property type="protein sequence ID" value="AAK42842"/>
        <property type="gene ID" value="SSO2732"/>
    </dbReference>
    <dbReference type="GeneID" id="1452761"/>
    <dbReference type="KEGG" id="sso:SSO2732"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2811"/>
    <dbReference type="eggNOG" id="arCOG01700">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VA3"/>
    <dbReference type="PhylomeDB" id="Q97VA3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11592">
        <property type="entry name" value="Agmatinase_PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005925">
        <property type="entry name" value="Agmatinase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01230">
        <property type="entry name" value="agmatinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="305" mass="34371" checksum="1A39497435E2E4E6" modified="2001-10-01" version="1">MRQLDALKSPRFTQISTFGRLPICEKNEEIKAAFLGIPFDDAVTYRPGARFGPMGIRQGSRLLRPYNQFLDTYPFDKLNACDMGDINVIPGYIEDTMNAIQTSLYEIISSKNLVPFIAGGDHSITLPILRTLYKKFGKINIVHFDSHYDFWDSYWGKKYTHGTWLRRAIEEGLIKEAVQGGIRASTFSKEDLRDKERLGIRSFTIRDLKYNLDTVIREINSLSGPTHVSIDIDVVDPAFAPGTGTPEVGGLTSFEIIEIVRKLRFDKLVGFDVVEVAPPYDMSEITAMLAANIIYEGMSVLSLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VF5</accession>
    <name>Q97VF5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter, ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2670</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90441">
        <property type="entry name" value="F90441"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VF5"/>
    <dbReference type="FunCoup" id="Q97VF5">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2670"/>
    <dbReference type="TCDB" id="3.A.1.5.9">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2670"/>
    <dbReference type="EnsemblBacteria" id="AAK42789">
        <property type="protein sequence ID" value="AAK42789"/>
        <property type="gene ID" value="SSO2670"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2670"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2753"/>
    <dbReference type="eggNOG" id="arCOG00182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="InParanoid" id="Q97VF5"/>
    <dbReference type="PhylomeDB" id="Q97VF5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019700">
        <property type="term" value="P:organic phosphonate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067:SF3">
        <property type="entry name" value="MALTOSE ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067">
        <property type="entry name" value="OLIGOPEPTIDE/DIPEPTIDE ABC TRANSPORTER, ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="48"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="362" mass="40533" checksum="50FDF81A7C4EBC97" modified="2001-10-01" version="1">MLSHNSLRYSKNLPCTININSLHTLLFSREEKSISNGYEYKCVMILEVHNLNVIYDEGNSRIIKAVNDVSFGVEKGEILGIIGESGSGKTTLISAILRAIRPPGKIISGKVIFNGMDIFSMTIDEFRKLLWKDISYVPQASQNALNPVLPISEIFYHEAISHGEADKKRVIERASELLKLVGLDPARVLKMYPFQLSGGMKQRVMIALSLLLNPKLILMDEPTSALDMLNQELLLKLIKNINQEMGVTIVYVTHDILNIAQIANRLLVMYKGYVMEEGKTEEIIKSPLNPYTSLLVSSIPSLKGEVKVINVPLDEPLVSKEKGCPFLARCSKAFGRCKEELPEIRLVYDRKVRCHLYGSNGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VI1</accession>
    <name>Q97VI1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rubrerythrin (Rr)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rr</name>
        <name evidence="2" type="ordered locus">SSO2642</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90438">
        <property type="entry name" value="D90438"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988550.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VI1"/>
    <dbReference type="STRING" id="273057.SSO2642"/>
    <dbReference type="PaxDb" id="273057-SSO2642"/>
    <dbReference type="EnsemblBacteria" id="AAK42763">
        <property type="protein sequence ID" value="AAK42763"/>
        <property type="gene ID" value="SSO2642"/>
    </dbReference>
    <dbReference type="GeneID" id="1452682"/>
    <dbReference type="KEGG" id="sso:SSO2642"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2725"/>
    <dbReference type="eggNOG" id="arCOG01097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095256_2_1_2"/>
    <dbReference type="InParanoid" id="Q97VI1"/>
    <dbReference type="PhylomeDB" id="Q97VI1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01041">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052753">
        <property type="entry name" value="Rbr2/Nigerythrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rr_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33746">
        <property type="entry name" value="RUBRERYTHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33746:SF4">
        <property type="entry name" value="RUBRERYTHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="1">
        <location>
            <begin position="3"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="144" mass="16034" checksum="413ABDCF0DB5E75B" modified="2001-10-01" version="1">MKELKGTKTAENLRHAFCGEAMANRRYLYFAKRADEEGYPEIAGLLRSIAEGETAHAFGHLDFIRQGGIGDPATDKPIGTLEQMLESAVAGETYEWTQMYPGYAKVAREEGFNEIAEWFETLARAEKSHAEKFTGVLNQLKGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VK7</accession>
    <name>Q97VK7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dipeptide ABC transporter permease protein (DppC-3)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dppC-3</name>
        <name evidence="9" type="ordered locus">SSO2617</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90435">
        <property type="entry name" value="B90435"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989341.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VK7"/>
    <dbReference type="FunCoup" id="Q97VK7">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2617"/>
    <dbReference type="PaxDb" id="273057-SSO2617"/>
    <dbReference type="EnsemblBacteria" id="AAK42737">
        <property type="protein sequence ID" value="AAK42737"/>
        <property type="gene ID" value="SSO2617"/>
    </dbReference>
    <dbReference type="GeneID" id="1454064"/>
    <dbReference type="KEGG" id="sso:SSO2617"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2698"/>
    <dbReference type="eggNOG" id="arCOG00748">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_1_2_2"/>
    <dbReference type="InParanoid" id="Q97VK7"/>
    <dbReference type="PhylomeDB" id="Q97VK7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050366">
        <property type="entry name" value="BP-dependent_transpt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386:SF1">
        <property type="entry name" value="D,D-DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DDPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN APPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="96"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="298" mass="32893" checksum="6F70D0929F79CB6B" modified="2001-10-01" version="1">MSESSGIRFMLKALIRDKAGLLGLIIVSLFLIWSLIQGILEILSSYLRKQYLGYILLPHNPFQYNLQLAFHPPSSTFLLGTNAEGEDILSRILYALPRDAFVAIVVVFSAIIIGGILGILAGYLGGIVDEILMRVTDAFLSLPALILVIAITVPLKATFFATILGLAVVWWPTYARFYRAQTLRIKNMDYISAAKLSNVSRISLFYRYIFLNAIDPVLAYAALDFGNVILTYSTLAFLGIGITPPIPELGEMAANGVTGLPQYWWWALFPGLTILIIVVGFVLLGDRLQDVVAGRIVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VL8</accession>
    <name>Q97VL8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transporter, ABC1 family, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2605</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90433">
        <property type="entry name" value="G90433"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989321.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VL8"/>
    <dbReference type="FunCoup" id="Q97VL8">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2605"/>
    <dbReference type="PaxDb" id="273057-SSO2605"/>
    <dbReference type="EnsemblBacteria" id="AAK42726">
        <property type="protein sequence ID" value="AAK42726"/>
        <property type="gene ID" value="SSO2605"/>
    </dbReference>
    <dbReference type="GeneID" id="1454053"/>
    <dbReference type="KEGG" id="sso:SSO2605"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2686"/>
    <dbReference type="eggNOG" id="arCOG01189">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006533_0_3_2"/>
    <dbReference type="InParanoid" id="Q97VL8"/>
    <dbReference type="PhylomeDB" id="Q97VL8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05121">
        <property type="entry name" value="ABC1_ADCK3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050154">
        <property type="entry name" value="UbiB_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566">
        <property type="entry name" value="CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566:SF113">
        <property type="entry name" value="PROTEIN ACTIVITY OF BC1 COMPLEX KINASE 7, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="460"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="104"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="496" mass="57516" checksum="DF5170C21E3DCEBB" modified="2001-10-01" version="1">MIRRLLEVFFKLAPRVLAYREFRNKILKEIPIDEKEMAEEAKKFVDTLIELGPTFIKFGQILSVRPDIMPETYIKELARLQDEVPPAPFSQVSKIIDEELGNSVKILKELSSASLGQVYLGEYNGKIVAIKVNRPGIKETVNEDIQVVKKLLPLLRFVFDESFIEIIKVFLEEFSRRIFEEMDYTKEAFYLNKIKEELSDYPSLRIPSIIKATKRVLVMEYIKGYKVTSEEAKKIVDNRILAYRVFRLFMYMLLNKDYFHADPHPGNIAVDEQGNLVLYDFGMSGKIDEKTRNLLIRAYVAMIRMDADSLVRVLDELGAIQPFADRRVLAKGLRLFMQAMQGIEVSELELEDFMKLADQVFFKFPLRMPSKLVLPFRMVNVLDGTCREIDKDFDFVKSSITFLEEEGYTTKVVIEQVRELVNGIWNRFRSFLLSYSQQQELINIQSSRKGSAITNYIPQMILVITIIFYAITKDIIITLLMVILAFSISLSGRKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VN5</accession>
    <name>Q97VN5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sulfolipid biosynthesis protein (SqdB)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">sqdB</name>
        <name evidence="3" type="ordered locus">SSO2583</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90431">
        <property type="entry name" value="E90431"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VN5"/>
    <dbReference type="FunCoup" id="Q97VN5">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2583"/>
    <dbReference type="PaxDb" id="273057-SSO2583"/>
    <dbReference type="EnsemblBacteria" id="AAK42708">
        <property type="protein sequence ID" value="AAK42708"/>
        <property type="gene ID" value="SSO2583"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2583"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2665"/>
    <dbReference type="eggNOG" id="arCOG04762">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040971_1_0_2"/>
    <dbReference type="InParanoid" id="Q97VN5"/>
    <dbReference type="PhylomeDB" id="Q97VN5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046507">
        <property type="term" value="F:UDPsulfoquinovose synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05255">
        <property type="entry name" value="SQD1_like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053578">
        <property type="entry name" value="UDP-sulfoquinovose_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041015">
        <property type="entry name" value="UDPsulfquin_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="389" mass="44686" checksum="1A63ADFF333A26C3" modified="2001-10-01" version="1">MMKVIILGADGYLGWALALRLAKRGHEVIGVDNLYTRDAVREVGSDSAFPLPDPEERISAARKLLDVDITFYRADVKDFDKLHEIIQRHKPEAIVHFAEQRSAPYSMIDVNHANYTLINNLTSTINLIYSIIKVDPSIHILKMGTMGEYGTPNYDILESPFVEVEINGKKDKIPFPKWASSWYHWSKVYDSYNLMWANKVWNLTITDIMQGPVYGTRTEEIIDEKLRTRFDFDEVWGTVVNRYCVEAVLGLPLTPYGKGGQTRGFISLEDSMQALTILLEHPPSQGEYRVVNQIHEVYSVGQIANMVKEVGESMGLTVKIQNVNNPRVEKEEHYYNVETKILPSLGFSPKKNLKNEIRNIIEDLIPYKNRLESFKHVILPKTDWRRGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VN9</accession>
    <name>Q97VN9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO10704</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99431">
        <property type="entry name" value="A99431"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923928.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VN9"/>
    <dbReference type="PaxDb" id="273057-SSO10704"/>
    <dbReference type="EnsemblBacteria" id="AAK42704">
        <property type="protein sequence ID" value="AAK42704"/>
        <property type="gene ID" value="SSO10704"/>
    </dbReference>
    <dbReference type="GeneID" id="1454031"/>
    <dbReference type="KEGG" id="sso:SSO10704"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2660"/>
    <dbReference type="eggNOG" id="arCOG03851">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165897_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VN9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="94" mass="11195" checksum="15F5D55EC80CBA25" modified="2001-10-01" version="1">MEPFYFKSYNRTVGIAHDINELEKEIERLGRDDPGCVEWHLREGHIVVWLNYIGERGLAEMLRGVGNVKEALARIREFKALKSRQRKRTRYYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VZ0</accession>
    <name>Q97VZ0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Phosphate permease, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO2461</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential transporter for phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99418">
        <property type="entry name" value="A99418"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923868.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VZ0"/>
    <dbReference type="FunCoup" id="Q97VZ0">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2461"/>
    <dbReference type="PaxDb" id="273057-SSO2461"/>
    <dbReference type="EnsemblBacteria" id="AAK42600">
        <property type="protein sequence ID" value="AAK42600"/>
        <property type="gene ID" value="SSO2461"/>
    </dbReference>
    <dbReference type="GeneID" id="7797350"/>
    <dbReference type="KEGG" id="sso:SSO2461"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2538"/>
    <dbReference type="eggNOG" id="arCOG02267">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015355_0_1_2"/>
    <dbReference type="InParanoid" id="Q97VZ0"/>
    <dbReference type="PhylomeDB" id="Q97VZ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF80">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="115"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="328" mass="35388" checksum="EEBD36B64A496D7A" modified="2001-10-01" version="1">MAITIIISMQNIIEYLLFIVGLISSYVIGANNNATSLGILFATNAIKRRYAYILNMVSILVGVVLGSLTMLHSVYGVVYGNSLYVAIGIFTSLSSSIITFYYLNKSGIPSSLSQTFYPSIAILTLISHGFIELDWVKFWVIVSSWVISPIVAIGFSLLLYLTLNKVVSAETRIIKQITIYKNLILFSSAFTSFIVGANAIGIIVSSALLSAPAYLVIPLYGISAALGVLTSQRIAIRVGFRITKLGYLGASSALIGSNVINEIFTILGIPLSITQTIIGGIVGLSFRSFTSDVRKQVIQIFRSWATSPIFAIILSLAIFGVIKSILGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W04</accession>
    <name>RTCA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>RNA 3'-terminal phosphate cyclase</fullName>
            <shortName>RNA cyclase</shortName>
            <shortName>RNA-3'-phosphate cyclase</shortName>
            <ecNumber>6.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rtcA</name>
        <name type="ordered locus">SSO2446</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.5.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK42586" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.5.1.4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42586.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90416">
        <property type="entry name" value="C90416"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993131.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W04"/>
    <dbReference type="SMR" id="Q97W04"/>
    <dbReference type="FunCoup" id="Q97W04">
        <property type="interactions" value="232"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2446"/>
    <dbReference type="PaxDb" id="273057-SSO2446"/>
    <dbReference type="EnsemblBacteria" id="AAK42586">
        <property type="protein sequence ID" value="AAK42586"/>
        <property type="gene ID" value="SSO2446"/>
    </dbReference>
    <dbReference type="GeneID" id="1453913"/>
    <dbReference type="GeneID" id="44128164"/>
    <dbReference type="KEGG" id="sso:SSO2446"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2524"/>
    <dbReference type="eggNOG" id="arCOG04125">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027882_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W04"/>
    <dbReference type="PhylomeDB" id="Q97W04"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003963">
        <property type="term" value="F:RNA-3'-phosphate cyclase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00874">
        <property type="entry name" value="RNA_Cyclase_Class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.20:FF:000002">
        <property type="entry name" value="RNA terminal phosphate cyclase-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00200">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017770">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03399">
        <property type="entry name" value="RNA_3prim_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF0">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005378">
        <property type="entry name" value="RNA3'_term_phos_cycl_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156436" description="RNA 3'-terminal phosphate cyclase">
        <location>
            <begin position="1"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="282"/>
            <end position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="337" mass="36845" checksum="53E7FD006751D9B0" modified="2002-05-27" version="2">MIEIDGSFGEGGGQILRTSLTLSVITGKPFRIFNIRANRPNPGLQRQHLWAVRAMKMISNAETKGDEVGSKELTFIPHEIKGNSIIDIDIGTAGSVTLIMQTMIPAIINKNMRIKIKGGTDVPKSPTIDYIRLVYLEILRKIGIESKVNLIKRGHYPEGGGEVIIENVNGNPSDFSLLELGKLIMIKGISHVSSLPSHIAERQKDSAKAILSKLGVQIEIETDVRQGEVSKGSGIALAAIGEKSIIGADSLGERGKRAEIVGEEAARKLIDNLNTKAAVDVHMSDMLMIFTSLFGGEYIGAELTSHAYTNMEIIRKFLDVKIEISGKRPFRFKAKIF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2017-03-15" modified="2025-06-18" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W08</accession>
    <name>TAW21_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA 4-demethylwyosine(37)-methyltransferase Taw21</fullName>
            <ecNumber evidence="3">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA(Phe):imG2 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">taw21</name>
        <name evidence="6" type="ordered locus">SSO2439</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2016" name="RNA" volume="22" first="1871" last="1883">
            <title>Evolution of tRNAPhe:imG2 methyltransferases involved in the biosynthesis of wyosine derivatives in Archaea.</title>
            <authorList>
                <person name="Urbonavicius J."/>
                <person name="Rutkiene R."/>
                <person name="Lopato A."/>
                <person name="Tauraite D."/>
                <person name="Stankeviciute J."/>
                <person name="Aucynaite A."/>
                <person name="Kaliniene L."/>
                <person name="van Tilbeurgh H."/>
                <person name="Meskys R."/>
            </authorList>
            <dbReference type="PubMed" id="27852927"/>
            <dbReference type="DOI" id="10.1261/rna.057059.116"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the C7-methylation of 4-demethylwyosine (imG-14) at position 37 in tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>4-demethylwyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = isowyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53056"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:13445"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:136979"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90415">
        <property type="entry name" value="G90415"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W08"/>
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    <dbReference type="EnsemblBacteria" id="AAK42582">
        <property type="protein sequence ID" value="AAK42582"/>
        <property type="gene ID" value="SSO2439"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2439"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2520"/>
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    <dbReference type="HOGENOM" id="CLU_022610_0_0_2"/>
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        <property type="organism ID" value="6163"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="term" value="F:tRNA methyltransferase activity"/>
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        <property type="term" value="P:tRNA N1-guanine methylation"/>
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    </dbReference>
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        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
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        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
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        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047729">
        <property type="entry name" value="tRNAMtaseTaw21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000439043" description="tRNA 4-demethylwyosine(37)-methyltransferase Taw21">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="148"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="175"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9V2G1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="27852927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="27852927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="262" mass="30468" checksum="4288B9F19B62E822" modified="2001-10-01" version="1">MKTLIKRLQDQQDIWKRIEIVGDIVIIGVPFNKKPEDLVEIANEILSTFPYVKSVWGRHRDVNGTYRLSTYVHLAGEKRSETVYKEHKCKYFLDFTKVFFSEKLSYEHLRVATQVKRDEIIINMFSGFGPFSILSAVLGRPKIVYSIDLNPYAYYYMMVNVELNKAYEVLPIYGDAFKRIYELEDADRIIAPLPELADKAYEVALQKVKKGGIIHLYTEVETNKGEDPVRIAMNKYRGSYFGRIVRSVNPHKYHVVVDIKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W11</accession>
    <name>Q97W11_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1404 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2436</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="D90415"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989385.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W11"/>
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    <dbReference type="EnsemblBacteria" id="AAK42579">
        <property type="protein sequence ID" value="AAK42579"/>
        <property type="gene ID" value="SSO2436"/>
    </dbReference>
    <dbReference type="GeneID" id="1453906"/>
    <dbReference type="KEGG" id="sso:SSO2436"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2516"/>
    <dbReference type="eggNOG" id="arCOG07321">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1393509_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009844">
        <property type="entry name" value="DUF1404"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07185">
        <property type="entry name" value="DUF1404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="22074" checksum="A4D9D68CC38DF3ED" modified="2001-10-01" version="1">MDVVQIAKYIGFSLLIISIIVYVFVDPVDRLLSYQGPILSGGLLGWYTLISNTPREKFIENDGEKIPVVSLLMRKRAIIFVTLGLVLIIPWLMPQIYPVVLKIQWLFVISFLCEFLGGFVIGYIIPSLKFTEKLLLFSLGFAGDTIYLLLLYLASDIFNIPYQLLLNSVIILVYGIKFPEGIVFAIYIMRKIGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W17</accession>
    <name>Q97W17_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Elongation factor 1-alpha, putative (TuF-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tuF-2</name>
        <name evidence="6" type="ordered locus">SSO2429</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AAK42573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90414">
        <property type="entry name" value="F90414"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989396.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="Q97W17">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2429"/>
    <dbReference type="PaxDb" id="273057-SSO2429"/>
    <dbReference type="EnsemblBacteria" id="AAK42573">
        <property type="protein sequence ID" value="AAK42573"/>
        <property type="gene ID" value="SSO2429"/>
    </dbReference>
    <dbReference type="GeneID" id="1453900"/>
    <dbReference type="KEGG" id="sso:SSO2429"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2507"/>
    <dbReference type="eggNOG" id="arCOG01562">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012821_2_0_2"/>
    <dbReference type="InParanoid" id="Q97W17"/>
    <dbReference type="PhylomeDB" id="Q97W17"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04165">
        <property type="entry name" value="GTPBP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03694">
        <property type="entry name" value="GTPBP_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03708">
        <property type="entry name" value="GTPBP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000014">
        <property type="entry name" value="Probable GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035531">
        <property type="entry name" value="GTPBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF9">
        <property type="entry name" value="GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="5">
        <location>
            <begin position="109"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="523" mass="58230" checksum="72A3853FD536FC27" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W55</accession>
    <name>Q97W55_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2385</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90409">
        <property type="entry name" value="F90409"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989456.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W55"/>
    <dbReference type="FunCoup" id="Q97W55">
        <property type="interactions" value="275"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2385"/>
    <dbReference type="PaxDb" id="273057-SSO2385"/>
    <dbReference type="EnsemblBacteria" id="AAK42533">
        <property type="protein sequence ID" value="AAK42533"/>
        <property type="gene ID" value="SSO2385"/>
    </dbReference>
    <dbReference type="GeneID" id="1453860"/>
    <dbReference type="KEGG" id="sso:SSO2385"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2467"/>
    <dbReference type="eggNOG" id="arCOG00358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044997_0_1_2"/>
    <dbReference type="InParanoid" id="Q97W55"/>
    <dbReference type="PhylomeDB" id="Q97W55"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01666">
        <property type="entry name" value="TGS_DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000016">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGS" evidence="3">
        <location>
            <begin position="276"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="351" mass="39917" checksum="046A96BF004865DE" modified="2001-10-01" version="1">MVTNLPAEAKAKWLKVMDAKTPEEKFQALQEFLKEVPKHKGTENLVYWAKRRMAELREEMEVRKSKKSGGFSLFVEKEGAGQAILIGDMLLRSFIMRKLTNVKQEFNELPVPGMVKYEDVQIQLVNPPKSLPLSKTIGLIRNADEVIIAINNKEDLSLIKSLLEDNNILLRKPKGRVVIERTRYGISGIRVVNLGKLIGIDEKVVRDYIESFGIKSAIVKIIGEVTLDDIEKSMFEAVSYKPTVVISKDRFDTGELPMLTIDQIDKLPKHLFDMLEVIRVYTKEPGEEPTKDPLILKKGSTVLDVARKLHSSLAENFRYARVWGKSVKFQGQKVGPSHILEDRDIVEIHVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-16" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W70</accession>
    <name>KHSE_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Homoserine kinase</fullName>
            <shortName evidence="1">HK</shortName>
            <shortName evidence="1">HSK</shortName>
            <ecNumber evidence="1">2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thrB</name>
        <name type="ordered locus">SSO2367</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-homoserine + ATP = O-phospho-L-homoserine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. Homoserine kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.39" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90407">
        <property type="entry name" value="G90407"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989478.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W70"/>
    <dbReference type="SMR" id="Q97W70"/>
    <dbReference type="FunCoup" id="Q97W70">
        <property type="interactions" value="209"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2367"/>
    <dbReference type="PaxDb" id="273057-SSO2367"/>
    <dbReference type="EnsemblBacteria" id="AAK42518">
        <property type="protein sequence ID" value="AAK42518"/>
        <property type="gene ID" value="SSO2367"/>
    </dbReference>
    <dbReference type="GeneID" id="1453845"/>
    <dbReference type="KEGG" id="sso:SSO2367"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2452"/>
    <dbReference type="eggNOG" id="arCOG01027">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041243_1_1_2"/>
    <dbReference type="InParanoid" id="Q97W70"/>
    <dbReference type="PhylomeDB" id="Q97W70"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004413">
        <property type="term" value="F:homoserine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00384">
        <property type="entry name" value="Homoser_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000870">
        <property type="entry name" value="Homoserine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002288">
        <property type="entry name" value="PRK01212.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00191">
        <property type="entry name" value="thrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF1">
        <property type="entry name" value="HOMOSERINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000676">
        <property type="entry name" value="Homoser_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00958">
        <property type="entry name" value="HOMSERKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0791">Threonine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156650" description="Homoserine kinase">
        <location>
            <begin position="1"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00384"/>
        </source>
    </evidence>
    <sequence length="311" mass="33649" checksum="4E39A20E5F83D507" modified="2001-10-01" version="1">MECKRARAYSSSANLGSGFDILSIAHTAFFDTVEICVENKNLNNIIVESNSKIPLEPNKNSATYPIVKIMEEIGIKASLKVRVIKGIPEGLGLGSSGASAAAAVMAFNNLFNLNLSKEDLVRYAMYGEIASSGSPHPDNVAASVFGGVVSVVSVSPVKVVEIPINYSFDILLFTPLNVHIEEKTKKAREMVPKTVTLSDYINNSRYISSLLLGFIKGERELIRLGLNDKIVEKARLPLFPYYPKIKEVAIKYDAIGACVSGAGPSILVLTDKMTDENKIVEEGTKTCNEFNVECKVIKAKIAGGVGIEGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W90</accession>
    <name>Q97W90_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulatory protein, AsnC family (AsnC)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">asnC</name>
        <name evidence="6" type="ordered locus">SSO2347</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90405">
        <property type="entry name" value="C90405"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989511.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W90"/>
    <dbReference type="STRING" id="273057.SSO2347"/>
    <dbReference type="PaxDb" id="273057-SSO2347"/>
    <dbReference type="EnsemblBacteria" id="AAK42498">
        <property type="protein sequence ID" value="AAK42498"/>
        <property type="gene ID" value="SSO2347"/>
    </dbReference>
    <dbReference type="GeneID" id="1453825"/>
    <dbReference type="KEGG" id="sso:SSO2347"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2431"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_4_2"/>
    <dbReference type="InParanoid" id="Q97W90"/>
    <dbReference type="PhylomeDB" id="Q97W90"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF8">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR PTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="5">
        <location>
            <begin position="8"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="153" mass="17786" checksum="58D5120AAAA97A1E" modified="2001-10-01" version="1">MNSSFYYLDDIDKKILNILQQDSRIPFSRLAKMLNLSEATIYVRIKRLKENGVIKGFYTEIDFDKIGLSVVAFILIKADPKKYDNILKQLVEMKEIYEIYDVTGEYYAVLKVRVPTREDLAKVLDKIGNMDGVTSTYTMLVLRSIKDKKELDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WB6</accession>
    <name>Q97WB6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flagella-related protein H, putative (FlaH)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">FlaH</name>
        <name evidence="4" type="ordered locus">SSO2318</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90402">
        <property type="entry name" value="A90402"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992492.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WB6"/>
    <dbReference type="STRING" id="273057.SSO2318"/>
    <dbReference type="PaxDb" id="273057-SSO2318"/>
    <dbReference type="EnsemblBacteria" id="AAK42472">
        <property type="protein sequence ID" value="AAK42472"/>
        <property type="gene ID" value="SSO2318"/>
    </dbReference>
    <dbReference type="GeneID" id="1453799"/>
    <dbReference type="KEGG" id="sso:SSO2318"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2406"/>
    <dbReference type="eggNOG" id="arCOG04148">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094838_1_0_2"/>
    <dbReference type="InParanoid" id="Q97WB6"/>
    <dbReference type="PhylomeDB" id="Q97WB6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004723">
        <property type="entry name" value="PRK06067.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF3">
        <property type="entry name" value="FLAGELLA-RELATED PROTEIN H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KaiC-like" evidence="3">
        <location>
            <begin position="8"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="234" mass="26062" checksum="A459CFA0B1F3AEBE" modified="2001-10-01" version="1">MEGYTVIIKTGNEDLDRRLSGLPFPALIMIEGDHGTGKSVLSAQFCYGLLIGGKKGYIITTEQTSKDYLKKMKDVKIDLIPFFLKGVLGIAPLNTNKFNWNSRLANKILEIIIDFIKRRKNMDFIIIDSLSIVATFAEIKQILQFMKDARVLVDLGKLILFTVHPDVFNEELKSRITSIVDVYFKLSATSIGGRRIKVLERIKTIGGIQGADAISFDIDPALGIKVVPLSLSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WC0</accession>
    <name>Q97WC0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposon ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2312</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90401">
        <property type="entry name" value="E90401"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923828.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WC0"/>
    <dbReference type="STRING" id="273057.SSO2312"/>
    <dbReference type="PaxDb" id="273057-SSO2312"/>
    <dbReference type="EnsemblBacteria" id="AAK42468">
        <property type="protein sequence ID" value="AAK42468"/>
        <property type="gene ID" value="SSO2312"/>
    </dbReference>
    <dbReference type="GeneID" id="1453795"/>
    <dbReference type="KEGG" id="sso:SSO2312"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2402"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066381_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WC0"/>
    <dbReference type="PhylomeDB" id="Q97WC0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="3">
        <location>
            <begin position="194"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="316" mass="35969" checksum="CCEDFFD1C808E95D" modified="2001-10-01" version="1">MEAPVAGIDVSKDKLVVYFQGKYYEFQNNKQGFEGVKQVLPKGCKVAIESTGVYHINLAKYLGKEYDVRIINPLVLKKFKNIRGKKSDKNDAKKLAELVISMGSEFTTSEAKELTSQWDFITRSIVRVKNRLRRDLILLGYKDSLSKKNLNEVLRGEDNIVLSEVRFLLDELERLEVRKREIEEELEDLVPKDSLIFTIPGIGRTLGCIILARVGDVKRFGDKKRFVAYCGLDPLVESSGKSVVSRGISRRGDAVLRRAFYLAALTAIKVNPIIKRFYEEHKENLKGKKLITACARKLAVITWAVLYYNKPFDASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WG7</accession>
    <name>Q97WG7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ferritin-like diiron domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2253</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90395">
        <property type="entry name" value="E90395"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WG7"/>
    <dbReference type="STRING" id="273057.SSO2253"/>
    <dbReference type="PaxDb" id="273057-SSO2253"/>
    <dbReference type="EnsemblBacteria" id="AAK42420">
        <property type="protein sequence ID" value="AAK42420"/>
        <property type="gene ID" value="SSO2253"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2253"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2347"/>
    <dbReference type="eggNOG" id="arCOG01098">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100475_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WG7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="2">
        <location>
            <begin position="4"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="80"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="185" mass="20822" checksum="AF8DE7C85ED83F81" modified="2001-10-01" version="1">MFVMALGKETEMGLKELFKANAEDFMSLMLVAEKLEQLGKKEEAKALREKALVELGHAKAIFETLLKNQELRGIVGEMAKEEQEQHVSEYNKVAMTAKQEGHEDIEKMLCSFADQEAKIAETIAKVSKVLNEMAKEEQEQHVSEYNKVAMTAKQEGHEDIEKMLCAFAEQEAKISETIKSVAKAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-06" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WI6</accession>
    <name>NCPP_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable inosine/xanthosine triphosphatase</fullName>
            <shortName evidence="1">ITPase/XTPase</shortName>
            <ecNumber evidence="1">3.6.1.73</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Non-canonical purine NTP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-standard purine NTP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-triphosphate phosphatase</fullName>
            <shortName evidence="1">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">SSO2230</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP to their respective diphosphate derivatives. Probably excludes non-canonical purines from DNA/RNA precursor pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>XTP + H2O = XDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28406"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59884"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ITP + H2O = IDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28330"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58280"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YjjX NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.73" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90393">
        <property type="entry name" value="A90393"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992042.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WI6"/>
    <dbReference type="SMR" id="Q97WI6"/>
    <dbReference type="FunCoup" id="Q97WI6">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2230"/>
    <dbReference type="PaxDb" id="273057-SSO2230"/>
    <dbReference type="EnsemblBacteria" id="AAK42400">
        <property type="protein sequence ID" value="AAK42400"/>
        <property type="gene ID" value="SSO2230"/>
    </dbReference>
    <dbReference type="GeneID" id="1453727"/>
    <dbReference type="KEGG" id="sso:SSO2230"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2327"/>
    <dbReference type="eggNOG" id="arCOG01221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087417_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WI6"/>
    <dbReference type="PhylomeDB" id="Q97WI6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103023">
        <property type="term" value="F:ITPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000002">
        <property type="entry name" value="Inosine/xanthosine triphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00648">
        <property type="entry name" value="Non_canon_purine_NTPase_YjjX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002786">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026533">
        <property type="entry name" value="NTPase/PRRC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050299">
        <property type="entry name" value="YjjX_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34699">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34699:SF2">
        <property type="entry name" value="NON-CANONICAL PURINE NTP PHOSPHATASE_PRRC1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01931">
        <property type="entry name" value="NTPase_I-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156364" description="Probable inosine/xanthosine triphosphatase">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00648"/>
        </source>
    </evidence>
    <sequence length="176" mass="19011" checksum="2DA06EB74FB4844B" modified="2001-10-01" version="1">MVTIALGSKNPVKISATKEALEILRLNWDLIATDIDSGVDKQPFCDQTYVGARNRALNAIKATNADIGLGIEGGVCNVYGKFIANAVVYVITKEGVENFAISSSFTLPSSIVSLILQGKELGEASDIIFKTINSKTKEGAVGLLTNNIIDRKTLYVQPIILALYPIYNTIINNTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WI7</accession>
    <name>Q97WI7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,4-dihydroxy-6-naphtoate synthase</fullName>
            <ecNumber evidence="3">4.1.99.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Menaquinone biosynthetic enzyme MqnD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mqnD</name>
        <name evidence="4" type="ordered locus">SSO2229</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of cyclic dehypoxanthine futalosine (cyclic DHFL) into 1,4-dihydroxy-6-naphthoate, a step in the biosynthesis of menaquinone (MK, vitamin K2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>cyclic dehypoxanthinylfutalosinate = 1,4-dihydroxy-6-naphthoate + dihydroxyacetone</text>
            <dbReference type="Rhea" id="RHEA:33087"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:64254"/>
            <dbReference type="ChEBI" id="CHEBI:64270"/>
            <dbReference type="EC" id="4.1.99.29"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MqnA/MqnD family. MqnD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.29" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90392">
        <property type="entry name" value="H90392"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992044.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WI7"/>
    <dbReference type="STRING" id="273057.SSO2229"/>
    <dbReference type="PaxDb" id="273057-SSO2229"/>
    <dbReference type="EnsemblBacteria" id="AAK42399">
        <property type="protein sequence ID" value="AAK42399"/>
        <property type="gene ID" value="SSO2229"/>
    </dbReference>
    <dbReference type="GeneID" id="1453726"/>
    <dbReference type="KEGG" id="sso:SSO2229"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2326"/>
    <dbReference type="eggNOG" id="arCOG00654">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_945334_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WI7"/>
    <dbReference type="PhylomeDB" id="Q97WI7"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13534">
        <property type="entry name" value="PBP2_MqnD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00996">
        <property type="entry name" value="MqnD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003773">
        <property type="entry name" value="Menaquinone_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030869">
        <property type="entry name" value="MqnD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37167">
        <property type="entry name" value="1,4-DIHYDROXY-6-NAPHTOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37167:SF1">
        <property type="entry name" value="1,4-DIHYDROXY-6-NAPHTOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02621">
        <property type="entry name" value="VitK2_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 3" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="113"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="288" mass="32811" checksum="7D0648B49E8347F8" modified="2001-10-01" version="1">MVTIRVGALADSGDLYPFIPLMEGKVKPEGFNLEFEVIPTVQDINEAVLKKEVDVSVPSVAMYPYIQDDYYILSNAVATAVDGITGMPILAIKEMSEDEIKRSRLIVHGPNTTAFTLYRLLIGKYGRLIIVRRVLDEIKALGKEGDVLVAVHEIKMMYALRKMGISVVRIGSMWDMWKTISNNTPMPMGMVVISKEVGKDLALRFKETYEKSKKYAEKHLDEIIPKDVQIMREAQKADLDEEIVRKTIWADIQEYNVPVDEIRRGLEFFFKITEEKGILPRVKNIDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WP4</accession>
    <name>Q97WP4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DedA family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90385">
        <property type="entry name" value="G90385"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992141.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WP4"/>
    <dbReference type="PaxDb" id="273057-SSO2166"/>
    <dbReference type="EnsemblBacteria" id="AAK42342">
        <property type="protein sequence ID" value="AAK42342"/>
        <property type="gene ID" value="SSO2166"/>
    </dbReference>
    <dbReference type="GeneID" id="7805656"/>
    <dbReference type="KEGG" id="sso:SSO2166"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2260"/>
    <dbReference type="eggNOG" id="arCOG03119">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369584_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WP4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051311">
        <property type="entry name" value="DedA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709">
        <property type="entry name" value="ALKALINE PHOSPHATASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709:SF10">
        <property type="entry name" value="SNARE ASSOCIATED GOLGI PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="22168" checksum="21D5C37A63FF6B01" modified="2001-10-01" version="1">MLTAQELIVIFVISFITNATPFFGAPYTLITTSILIKSGVSPLSLILAVVLSGLGASLSKLVMYALGIAIRKPLKDNKNILLIEKISKSYGFYAALFILTILPFLPLDDYIFLAGGVARLSVFRMILISIIGKTLKSGIEISIELTGISLIASIFSINEFELSIISIIVFILLGILLFKLDWEEILKRGEKFLREKLKINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WT6</accession>
    <name>Q97WT6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-haloalkanoic acid dehalogenase</fullName>
            <ecNumber evidence="2">3.8.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.8.1.2" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90369">
        <property type="entry name" value="H90369"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923736.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WT6"/>
    <dbReference type="FunCoup" id="Q97WT6">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2028"/>
    <dbReference type="PaxDb" id="273057-SSO2028"/>
    <dbReference type="EnsemblBacteria" id="AAK42215">
        <property type="protein sequence ID" value="AAK42215"/>
        <property type="gene ID" value="SSO2028"/>
    </dbReference>
    <dbReference type="GeneID" id="7805871"/>
    <dbReference type="KEGG" id="sso:SSO2028"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2108"/>
    <dbReference type="eggNOG" id="arCOG02291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_8_0_2"/>
    <dbReference type="InParanoid" id="Q97WT6"/>
    <dbReference type="PhylomeDB" id="Q97WT6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018784">
        <property type="term" value="F:(S)-2-haloacid dehalogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051828">
        <property type="entry name" value="HAD-like_hydrolase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191:SF2">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="212" mass="24589" checksum="E4B52ED49BEF59CD" modified="2001-10-01" version="1">MYRAIFVDFGNTLVGFKPAFYEKLQTILREHGYDVDIRRVFRAYVKAMAVNNYSQPTDIKEFLYNLNIPPSDRLISHIRGSDIRDGEAFIYDDVMEFLETIRSTNTKLILLSNSSPRTKKLLEELGLVKYFDDLVLSHEIGIVKPNPKIFAIAILKGGYPALHIGDIYEIDYVGARRSYVDAILLDRYDFYPEIKEKVRNLREIIPMVTKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WU6</accession>
    <name>Q97WU6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ring oxydation complex/ phenylacetic acid degradation related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90368">
        <property type="entry name" value="E90368"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923726.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WU6"/>
    <dbReference type="STRING" id="273057.SSO2018"/>
    <dbReference type="PaxDb" id="273057-SSO2018"/>
    <dbReference type="EnsemblBacteria" id="AAK42204">
        <property type="protein sequence ID" value="AAK42204"/>
        <property type="gene ID" value="SSO2018"/>
    </dbReference>
    <dbReference type="GeneID" id="12414702"/>
    <dbReference type="KEGG" id="sso:SSO2018"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2094"/>
    <dbReference type="eggNOG" id="arCOG04786">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093768_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WU6"/>
    <dbReference type="PhylomeDB" id="Q97WU6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.10:FF:000027">
        <property type="entry name" value="Ring oxydation complex/ phenylacetic acid degradation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052703">
        <property type="entry name" value="Aromatic_CoA_ox/epox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007814">
        <property type="entry name" value="PaaA_PaaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30458:SF0">
        <property type="entry name" value="1,2-PHENYLACETYL-COA EPOXIDASE, SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30458">
        <property type="entry name" value="PHENYLACETIC ACID DEGRADATION PROTEIN PAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05138">
        <property type="entry name" value="PaaA_PaaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="256" mass="30052" checksum="606E8042A005C5C1" modified="2001-10-01" version="1">MMVVVEYKKPKRVVGWGEYKGLRKFESIYDLPPEAVEVLFKLLSVQGDTEFASIEQHMPWLIHAPKLSDRVTISRIMVDEMRHGWQVIQILKEFGEEGKKIAEDLLSRRMGRHKLDAFNIPFNMWEDTLGFTFLIDRVGMFQLLAFEDSSFGPLSRIIPTMMMEEEFHINFGYTGIKKLVEEGKRDLAQALINKWFPRGLDMFGHSKSITIDLAYKYGIKKMRNDEMRQLYIKDIKELITPLGLELPDINRNRRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WU7</accession>
    <name>Q97WU7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Enoyl CoA hydratase (PaaF-4)</fullName>
            <ecNumber evidence="4">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">paaF-4</name>
        <name evidence="4" type="ordered locus">SSO2017</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90368">
        <property type="entry name" value="D90368"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WU7"/>
    <dbReference type="STRING" id="273057.SSO2017"/>
    <dbReference type="PaxDb" id="273057-SSO2017"/>
    <dbReference type="EnsemblBacteria" id="AAK42203">
        <property type="protein sequence ID" value="AAK42203"/>
        <property type="gene ID" value="SSO2017"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2017"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2093"/>
    <dbReference type="eggNOG" id="arCOG00239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_2"/>
    <dbReference type="InParanoid" id="Q97WU7"/>
    <dbReference type="PhylomeDB" id="Q97WU7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000157">
        <property type="entry name" value="Enoyl CoA hydratase (PaaF-4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="270" mass="30070" checksum="A616E21CDB1DBF71" modified="2001-10-01" version="1">MRLMGLSELNPKYFKIEVEDGVGIIKLNRSPANAHNLEMLRELDNIIVESRFDQNVKAILITSNIPRFFSAGFDINEIKDKSPEYIGLSSQFSKEVMLRMMSTKKLIIASINGHCMGGGLELALASDLRFGANDENIKFGMPEVANLALIPGEGGTQFLARLVGRSKAIYLIVTGKTLSPKEAYELGILDRLIEPEKLFEESFEFARQVAKGPSLAVGFTKLAVNEGMDLPWYNAFALEREMQNQALASEDAKEGARAFFEKRRPVFKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WV4</accession>
    <name>Q97WV4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-methyl hydantoinase A (HuyA-2)</fullName>
            <ecNumber evidence="4">3.5.2.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">huyA-2</name>
        <name evidence="4" type="ordered locus">SSO2008</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.14" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90367">
        <property type="entry name" value="E90367"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923719.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WV4"/>
    <dbReference type="FunCoup" id="Q97WV4">
        <property type="interactions" value="187"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2008"/>
    <dbReference type="PaxDb" id="273057-SSO2008"/>
    <dbReference type="EnsemblBacteria" id="AAK42196">
        <property type="protein sequence ID" value="AAK42196"/>
        <property type="gene ID" value="SSO2008"/>
    </dbReference>
    <dbReference type="GeneID" id="1453523"/>
    <dbReference type="KEGG" id="sso:SSO2008"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2083"/>
    <dbReference type="eggNOG" id="arCOG01511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002157_1_2_2"/>
    <dbReference type="InParanoid" id="Q97WV4"/>
    <dbReference type="PhylomeDB" id="Q97WV4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047423">
        <property type="term" value="F:N-methylhydantoinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049517">
        <property type="entry name" value="ACX-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF23">
        <property type="entry name" value="HYPOTHETICAL 5-OXOPROLINASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19278">
        <property type="entry name" value="Hydant_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="1">
        <location>
            <begin position="193"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Acetophenone carboxylase-like C-terminal" evidence="3">
        <location>
            <begin position="483"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="644" mass="71776" checksum="BE33904EDCCD7436" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X44</accession>
    <name>Q97X44_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hemerythrin HHE cation binding domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1911</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90356">
        <property type="entry name" value="A90356"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X44"/>
    <dbReference type="PaxDb" id="273057-SSO1911"/>
    <dbReference type="EnsemblBacteria" id="AAK42104">
        <property type="protein sequence ID" value="AAK42104"/>
        <property type="gene ID" value="SSO1911"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1911"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1973"/>
    <dbReference type="eggNOG" id="arCOG06046">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131319_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X44"/>
    <dbReference type="PhylomeDB" id="Q97X44"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="20212" checksum="604327976F7A0AB0" modified="2001-10-01" version="1">MLVLEVIKISLLFLHFTPLNISFFNIPRLMKIMDIIELLKFEHGVFRIRFYFLEKIGDFWQELEALHNFIVNVHAKMEDLYVFKDIPEARPYSNDHKLIEKYGDSIIKEKRKDWVPRYVKIVLDHNLNEEKYIFPKVKEKKDLVLDIIEQYGFENYQKATGIDIRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X72</accession>
    <name>Q97X72_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Terminal quinol oxidase, subunit, putative (DoxD-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">doxD-like</name>
        <name evidence="3" type="ordered locus">SSO1881</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90351">
        <property type="entry name" value="G90351"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991418.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X72"/>
    <dbReference type="STRING" id="273057.SSO1881"/>
    <dbReference type="PaxDb" id="273057-SSO1881"/>
    <dbReference type="EnsemblBacteria" id="AAK42070">
        <property type="protein sequence ID" value="AAK42070"/>
        <property type="gene ID" value="SSO1881"/>
    </dbReference>
    <dbReference type="GeneID" id="1453397"/>
    <dbReference type="KEGG" id="sso:SSO1881"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1934"/>
    <dbReference type="eggNOG" id="arCOG02862">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1544122_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X72"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007301">
        <property type="entry name" value="DoxD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04173">
        <property type="entry name" value="DoxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="TQO small subunit DoxD" evidence="2">
        <location>
            <begin position="12"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="173" mass="19119" checksum="50E806243B9B6618" modified="2001-10-01" version="1">MAQVTIEQTQKFLPMLRVTLGLMFFSAFIRRAINVPAKLDPNSSAYVGGKLITFLPHAWSPIKGMLESILLNPSLLYDFIILFTAIEAIFGLFMIIGFLTRLSGLVLAGMAWGIGLAAGWLGSTCVDEWQIASVEGAAAFMFVFTGSRWLSIDYILAKKSKGIKIGKYYIPLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X80</accession>
    <name>Q97X80_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vitamin K epoxide reductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">SSO1874</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VKOR family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90350">
        <property type="entry name" value="G90350"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992687.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X80"/>
    <dbReference type="FunCoup" id="Q97X80">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1874"/>
    <dbReference type="PaxDb" id="273057-SSO1874"/>
    <dbReference type="EnsemblBacteria" id="AAK42062">
        <property type="protein sequence ID" value="AAK42062"/>
        <property type="gene ID" value="SSO1874"/>
    </dbReference>
    <dbReference type="GeneID" id="1453389"/>
    <dbReference type="KEGG" id="sso:SSO1874"/>
    <dbReference type="eggNOG" id="arCOG03749">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1901974_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X80"/>
    <dbReference type="PhylomeDB" id="Q97X80"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12918">
        <property type="entry name" value="VKOR_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.130">
        <property type="entry name" value="VKOR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012932">
        <property type="entry name" value="VKOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038354">
        <property type="entry name" value="VKOR_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011480">
        <property type="entry name" value="PRK14889.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07884">
        <property type="entry name" value="VKOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00756">
        <property type="entry name" value="VKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0874">Quinone</keyword>
    <keyword evidence="9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Vitamin K epoxide reductase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK42062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="126" mass="14452" checksum="2B5AC80610BDF263" modified="2001-10-01" version="1">MIKKIGIGFSLIGLIDSLYLFILTRLEKPLMYCNISSLVNCSKVEFSQFSTFLGIPDALLGTIFFSIMLILWILMFTEELKYLWIVGSVFTIYLIYTEILIGNICLYCTIAHLSCLIQGFIIFHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XB7</accession>
    <name>Q97XB7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99346">
        <property type="entry name" value="A99346"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923614.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XB7"/>
    <dbReference type="STRING" id="273057.SSO1827"/>
    <dbReference type="PaxDb" id="273057-SSO1827"/>
    <dbReference type="EnsemblBacteria" id="AAK42024">
        <property type="protein sequence ID" value="AAK42024"/>
        <property type="gene ID" value="SSO1827"/>
    </dbReference>
    <dbReference type="GeneID" id="1453351"/>
    <dbReference type="KEGG" id="sso:SSO1827"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1888"/>
    <dbReference type="eggNOG" id="arCOG09710">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1393637_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XB7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="195" mass="22169" checksum="F389D2F4576A1276" modified="2001-10-01" version="1">MEKFREEAILGTLLFLSSFLLHNQYFYLSIILVYALASYLGLKYIPPIVSSILLIFSSLHAFLVLIPYPLLFLDKRFTISFLLSTFFAMLTENYLILLLAFALDRRGLISSGLFLLILGGVFMNVEYGNAAFFLIVAGVISTLIETKIKIDHKTSAVVYLSSLLIFYKIPYLIPLLLCFSPLTSLLFSWVIHFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XI1</accession>
    <name>Q97XI1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protease, putative</fullName>
            <ecNumber evidence="1">3.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1759</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90337">
        <property type="entry name" value="A90337"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989064.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XI1"/>
    <dbReference type="PaxDb" id="273057-SSO1759"/>
    <dbReference type="EnsemblBacteria" id="AAK41952">
        <property type="protein sequence ID" value="AAK41952"/>
        <property type="gene ID" value="SSO1759"/>
    </dbReference>
    <dbReference type="GeneID" id="1454731"/>
    <dbReference type="KEGG" id="sso:SSO1759"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1805"/>
    <dbReference type="eggNOG" id="arCOG03202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053295_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XI1"/>
    <dbReference type="PhylomeDB" id="Q97XI1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001539">
        <property type="entry name" value="Peptidase_U32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051454">
        <property type="entry name" value="RNA/ubiquinone_mod_enzymes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30217:SF10">
        <property type="entry name" value="23S RRNA 5-HYDROXYCYTIDINE C2501 SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30217">
        <property type="entry name" value="PEPTIDASE U32 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01136">
        <property type="entry name" value="Peptidase_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="379" mass="43798" checksum="49C041F625FA0E16" modified="2001-10-01" version="1">MKLVVATNFDDSLLEGLKRYPDVKYIFGSFKRTITGHGRAGFIVPHIKEEQFETHISLAHSYGIKFLYTMNTNTLLGKEYDTEFIGKVMKEVDKLVNFGVDGFIVALPFLIRLIRTEYPDLEVSASSFSRIRNVREVEEYTNLGVNTIIMHEDANRDFKLLKEVAALSRANRFEIELILNNSCLYGCPFRLTHDNISSVTSMVNGVNDVWFEYPVLLCATDVLNDPANLIRSRWIRPEDIKYYEEIGINRFKIAGRNKKTDWILRVVKAYAERKYEGDLLDLVSYPQGRAATKAVQMVNGPSSYFILTSVRIDNTKFPKGWIKFFFTNDCDTRSCKECKYCDIVAERVMTVNGEPFKSSEWSIRQPYPINIIPKFKERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XP6</accession>
    <name>Q97XP6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytosine permease (CodB)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">codB</name>
        <name evidence="7" type="ordered locus">SSO1665</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90327">
        <property type="entry name" value="F90327"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990099.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XP6"/>
    <dbReference type="STRING" id="273057.SSO1665"/>
    <dbReference type="PaxDb" id="273057-SSO1665"/>
    <dbReference type="EnsemblBacteria" id="AAK41877">
        <property type="protein sequence ID" value="AAK41877"/>
        <property type="gene ID" value="SSO1665"/>
    </dbReference>
    <dbReference type="GeneID" id="1454656"/>
    <dbReference type="KEGG" id="sso:SSO1665"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1714"/>
    <dbReference type="eggNOG" id="arCOG03447">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035711_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XP6"/>
    <dbReference type="PhylomeDB" id="Q97XP6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015209">
        <property type="term" value="F:cytosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000026">
        <property type="entry name" value="Permease for cytosine/purines uracil thiamine allantoin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030191">
        <property type="entry name" value="CodB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30569:SF0">
        <property type="entry name" value="CYTOSINE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30569">
        <property type="entry name" value="CYTOSINE TRANSPORTER CODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="453" mass="48817" checksum="EF43F53B35471585" modified="2001-10-01" version="1">MTGKEEISSKYDDYSLKEVPKDSRYGFFNVFLVFSSVYGAIAVIWAGGALGYGLTFSQAIIAVLSGTVVLGILGSLTAAVGAYSGLSTYVMWRHPLGRWGGKIAGLLLITITTGIGWYAVETWLFGIVMSEIFPNNPFFSVGVAAIWGGILMTIMTYVGYRMLSFLSYFTIPFHIWLIAIGIAIVLALKGGFHTVMAAVPTSHMSLLDGISATIGLYSAGTIISPDISRFAKSAKDAGYAWFAHIIFLYPFLILGGVAIVLATGSYLITNAMLELGMGVGVLLIIVFGQFIINTDNLYSGSLSLVNLIPMRREIASVINGVIGTAIAAYVGFSAGSSITPFENFISLLGDFLPAMGGIVLADFYIVKKYVNKIQDPHKRYEFVPNNKYYNINIAGILALALGSIIGYFVNAGIPAINSLVTGFLSYIIIYYIIKAMGKSPEILPFNYEGGILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XP7</accession>
    <name>Q97XP7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH asnC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1664</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90327">
        <property type="entry name" value="E90327"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923555.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XP7"/>
    <dbReference type="STRING" id="273057.SSO1664"/>
    <dbReference type="PaxDb" id="273057-SSO1664"/>
    <dbReference type="EnsemblBacteria" id="AAK41876">
        <property type="protein sequence ID" value="AAK41876"/>
        <property type="gene ID" value="SSO1664"/>
    </dbReference>
    <dbReference type="GeneID" id="1454655"/>
    <dbReference type="KEGG" id="sso:SSO1664"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1713"/>
    <dbReference type="eggNOG" id="arCOG01587">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129671_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XP7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF8">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR PTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="11"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="159" mass="18294" checksum="997AD1EDFD8784E1" modified="2001-10-01" version="1">MSWEEVFRNYLDDKDIRILKLLNQDANISDAKLGKLVGLSKTAVRLRRVKLQNSGILKIVGVVVLQNLGIPYADLLVKLKNDIVLREQFINKLIANDLVYEVTEYMGDWDLLIRLFHNNIAKLKDESLKIISDKAVQDYRISYVVKTYKAWGKSILSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XS0</accession>
    <name>Q97XS0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">First ORF in transposon ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO8568</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90324">
        <property type="entry name" value="E90324"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XS0"/>
    <dbReference type="PaxDb" id="273057-SSO8568"/>
    <dbReference type="EnsemblBacteria" id="AAK41852">
        <property type="protein sequence ID" value="AAK41852"/>
        <property type="gene ID" value="SSO8568"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO8568"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1685"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170813_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XS0"/>
    <dbReference type="PhylomeDB" id="Q97XS0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="81" mass="9540" checksum="21263C32917623BB" modified="2001-10-01" version="1">MRRYENSKKGYEEILKMKPCVIVLEPTGVYAIRPSQYFKEKGIKVLQVSPNVLSREKELRGKKTDFYDAEKLENMVNRAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XS2</accession>
    <name>Q97XS2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1637</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90324">
        <property type="entry name" value="C90324"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923537.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XS2"/>
    <dbReference type="PaxDb" id="273057-SSO1637"/>
    <dbReference type="DNASU" id="1454629"/>
    <dbReference type="EnsemblBacteria" id="AAK41850">
        <property type="protein sequence ID" value="AAK41850"/>
        <property type="gene ID" value="SSO1637"/>
    </dbReference>
    <dbReference type="GeneID" id="12417821"/>
    <dbReference type="KEGG" id="sso:SSO1637"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1682"/>
    <dbReference type="eggNOG" id="arCOG07234">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2127951_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XS2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="114" mass="12581" checksum="11E9FABB5A7E2E64" modified="2001-10-01" version="1">MKGTNTIYIIISSILLAYIMQIFVLYPFTAVAIGIPLGLLSRKYSAIGGFLIGFLSSLSLYLIYPINAVSKIAEIIGQLIGINPFLVVLLYPLIYGTISLISALLFYYIVRLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XT5</accession>
    <name>Q97XT5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1622</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90322">
        <property type="entry name" value="F90322"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923524.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XT5"/>
    <dbReference type="STRING" id="273057.SSO1622"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1622"/>
    <dbReference type="EnsemblBacteria" id="AAK41837">
        <property type="protein sequence ID" value="AAK41837"/>
        <property type="gene ID" value="SSO1622"/>
    </dbReference>
    <dbReference type="GeneID" id="1454616"/>
    <dbReference type="KEGG" id="sso:SSO1622"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1665"/>
    <dbReference type="eggNOG" id="arCOG01417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_654920_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XT5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="241"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="419" mass="49221" checksum="FA46CFB0BB596A98" modified="2001-10-01" version="1">MTNMLKIRAPNIFMKDYVGGAEYVTLNWLKRFKKIEYEIVPDVDLVYLDLCMNKKVQKDNILEIIDKLKLHVDNVVLDYYLDCKLHNNTDIFKKILDNEIILDMKIGTNVNISQFFNKHILEFINGRPVGYDFLYYSLKYSKKYIGILQHLGDIPISLKFYIYHFKELLNYLESLAELMYLLASVIREKTWVQALIHNKDKVHLLTPSRGAIKKAGLDKLNYTVIFPYVGLDSTILNYSCKNKDNYAVFFARPYRTKGILEAIKIFKLARKKGYLQRLYIIGGNINDITSKYNNKSDIVNLGRIKDKNEVYKILSKAQMNIYPSISDAFGITILESLAVYTPVIMYYNEANYEYFSKSKLVKIVKLFNKYEFLNKINEIINNNLEIDEYTYNLILSHLDWNKIVNNIEEHLLNIINDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XX2</accession>
    <name>Q97XX2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90317">
        <property type="entry name" value="F90317"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XX2"/>
    <dbReference type="STRING" id="273057.SSO1588"/>
    <dbReference type="PaxDb" id="273057-SSO1588"/>
    <dbReference type="EnsemblBacteria" id="AAK41797">
        <property type="protein sequence ID" value="AAK41797"/>
        <property type="gene ID" value="SSO1588"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1588"/>
    <dbReference type="HOGENOM" id="CLU_2257493_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XX2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="103" mass="11925" checksum="C0917F3C373D48E7" modified="2001-10-01" version="1">MIMMIRSITHQEVPTAISTQIPLPTQRPTTLNYMMRTTRGARNILLVTGFLSISNTQYININSLMMTLSIHSTIFVISSLYMRFRFGICELLDSLMLLRYNDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XX6</accession>
    <name>Q97XX6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF973 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1583</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90317">
        <property type="entry name" value="B90317"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923483.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XX6"/>
    <dbReference type="STRING" id="273057.SSO1583"/>
    <dbReference type="PaxDb" id="273057-SSO1583"/>
    <dbReference type="DNASU" id="1454572"/>
    <dbReference type="EnsemblBacteria" id="AAK41793">
        <property type="protein sequence ID" value="AAK41793"/>
        <property type="gene ID" value="SSO1583"/>
    </dbReference>
    <dbReference type="GeneID" id="7797633"/>
    <dbReference type="KEGG" id="sso:SSO1583"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1617"/>
    <dbReference type="eggNOG" id="arCOG03768">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075237_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XX6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009321">
        <property type="entry name" value="DUF973"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06157">
        <property type="entry name" value="DUF973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="30711" checksum="CF5E99A7B7EBEBA5" modified="2001-10-01" version="1">MSQQNTELEGIGKLRSGSLFMILAVLLAAIGILVIISAGMLGGMFSAASGNVSGVIASGIGLLVGIAIVILIGAIIGLIGILRIRSGFGILKSLGRDVGIGEIGTTLYLVGLIIIIIGALLTIVLIGFPILILGEIIALIGGILIGIGFYKVGEIYNEGLVKIGGILIVIPIDLINFVGFILAYVGLGKVRPLPTVAQQPLVPQVYQVGQGTIRNNGYAYITLYSSTPASIISAKIEGANIMSSAINPTVLQIGNNEVTIFFGNVQSLAPNTTYIITLTINIGGNIINISTTAVYQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XZ4</accession>
    <name>Q97XZ4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Orf in partial transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99315">
        <property type="entry name" value="B99315"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923471.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XZ4"/>
    <dbReference type="PaxDb" id="273057-SSO1564"/>
    <dbReference type="EnsemblBacteria" id="AAK41777">
        <property type="protein sequence ID" value="AAK41777"/>
        <property type="gene ID" value="SSO1564"/>
    </dbReference>
    <dbReference type="GeneID" id="1454556"/>
    <dbReference type="KEGG" id="sso:SSO1564"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1599"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="InParanoid" id="Q97XZ4"/>
    <dbReference type="PhylomeDB" id="Q97XZ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04762">
        <property type="entry name" value="HTH_MerR-trunc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2170:FF:000002">
        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1390:FF:000002">
        <property type="entry name" value="ORF1 in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="55"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="191" mass="22394" checksum="1C73BCE1D9933BF0" modified="2001-10-01" version="1">MLRPKEVCQRLGISYPTLREYVKKGYIKPVILQSGKWRFNEEDVEKLMGIVKRRKVVLYARVSSKQKDELVNQVKYLEEQVKEYDLVITDIGSGLNMKRKGFLKLLRMILNNEVSRVVIAYPDRLVRFGFEILEETCKAHGCEIVVLNQEEKEEELVEDLISVLVSFSEKLHGMRSHEYEKVKKCAEELKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y74</accession>
    <name>Q97Y74_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1468</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90304">
        <property type="entry name" value="H90304"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990770.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y74"/>
    <dbReference type="STRING" id="273057.SSO1468"/>
    <dbReference type="PaxDb" id="273057-SSO1468"/>
    <dbReference type="EnsemblBacteria" id="AAK41695">
        <property type="protein sequence ID" value="AAK41695"/>
        <property type="gene ID" value="SSO1468"/>
    </dbReference>
    <dbReference type="GeneID" id="1454474"/>
    <dbReference type="KEGG" id="sso:SSO1468"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1502"/>
    <dbReference type="eggNOG" id="arCOG03167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041527_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y74"/>
    <dbReference type="PhylomeDB" id="Q97Y74"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025420">
        <property type="entry name" value="DUF4143"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295:SF19">
        <property type="entry name" value="ARCHAEAL ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13635">
        <property type="entry name" value="DUF4143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="41"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4143" evidence="2">
        <location>
            <begin position="214"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="48256" checksum="F38BE8D3BF154A97" modified="2001-10-01" version="1">MYIEEIKRVLSDQRDVLEEKMSRKLVERDVPNLLKYLEVPNALAILGVRRSGKSTLSLLLLKRKKFAYVNFDDERFYNLNIGDLNKVLQAIYELYGVDVDYIVLDEIHNVKGWELFVSRLRDVKKLIITGSNSKMLSGELATYLTGRHSDYILFPFSFQEYLKYKEVKVEYFSTRVISTLKNELEKYTEVGGFPEALMLGKEQVNVIYNDILFKDIVSRYKIREIEKFREFARTVISYYSNEISLSSIAKILGLNKRTVEKWASGLREAYLTFFIPRYGEKLKQRLTYNKKVYVVDVGIVSNLAIKGKDKGRIIENLVAIKLLRELQGEEKLYYMRNNYEVDFYDEKNSRLIQVTYTSDKIENREIRGLIEGYRLTKAKKLLIITWDLEEKIQVEGVNIEVLPLYRFLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YB8</accession>
    <name>Q97YB8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1058</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90298">
        <property type="entry name" value="F90298"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YB8"/>
    <dbReference type="PaxDb" id="273057-SSO1410"/>
    <dbReference type="EnsemblBacteria" id="AAK41645">
        <property type="protein sequence ID" value="AAK41645"/>
        <property type="gene ID" value="SSO1410"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1410"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1435"/>
    <dbReference type="eggNOG" id="arCOG02754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062982_1_2_2"/>
    <dbReference type="InParanoid" id="Q97YB8"/>
    <dbReference type="PhylomeDB" id="Q97YB8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053172">
        <property type="entry name" value="Tn903_transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34631:SF3">
        <property type="entry name" value="ISSOD12 TRANSPOSASE TNPA_ISSOD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="109"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="262" mass="30764" checksum="FD087D1EE9501A65" modified="2001-10-01" version="1">MGKSKYKRDWSKYDENVITRYKLMFPFYVFEHWWDLLAEENRNARTKYKAPKEFNEFLAFLHIFLPYRAIEGVLRALEQLKIIPTSLDYSTIWERVRNMNITFPEASDELEVIADATGIGTNTGGQYIVAKWGKTRDSKFLKIEIVMDNNEFNVVNAEVTSNEVESVKTVKDLQDKGKKFYGDKAYDANEVYKTGVEVVVPPKKNASTKRHLARRKAVREFRKLGYDRWREEKGYGVRWRVESLFSVKRTFGESVRQVLLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YC4</accession>
    <name>Q97YC4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ATP-dependent RNA helicase related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1402</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90297">
        <property type="entry name" value="F90297"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YC4"/>
    <dbReference type="STRING" id="273057.SSO1402"/>
    <dbReference type="PaxDb" id="273057-SSO1402"/>
    <dbReference type="EnsemblBacteria" id="AAK41637">
        <property type="protein sequence ID" value="AAK41637"/>
        <property type="gene ID" value="SSO1402"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1402"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1418"/>
    <dbReference type="eggNOG" id="arCOG01444">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031100_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YC4"/>
    <dbReference type="PhylomeDB" id="Q97YC4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054712">
        <property type="entry name" value="Cas3-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006474">
        <property type="entry name" value="Helicase_Cas3_CRISPR-ass_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01587">
        <property type="entry name" value="cas3_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF16">
        <property type="entry name" value="CRISPR-ASSOCIATED NUCLEASE_HELICASE CAS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22590">
        <property type="entry name" value="Cas3-like_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="43"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="397"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="572" mass="65544" checksum="5A2CACD9E456A75B" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YD9</accession>
    <name>Q97YD9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second part of one of two inversely orientated ORFs in a partial ISC1043</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO7896</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90295">
        <property type="entry name" value="G90295"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YD9"/>
    <dbReference type="STRING" id="273057.SSO7896"/>
    <dbReference type="PaxDb" id="273057-SSO7896"/>
    <dbReference type="EnsemblBacteria" id="AAK41622">
        <property type="protein sequence ID" value="AAK41622"/>
        <property type="gene ID" value="SSO7896"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7896"/>
    <dbReference type="HOGENOM" id="CLU_3113256_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YD9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="50" mass="5715" checksum="4888C1CBFFE597DA" modified="2001-10-01" version="1">MKKEYTKFSLPLSLPWISLFHSCNAWLLLGNNPSMVASILRSSTVFESRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YG6</accession>
    <name>Q97YG6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1356</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90292">
        <property type="entry name" value="A90292"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YG6"/>
    <dbReference type="STRING" id="273057.SSO1356"/>
    <dbReference type="PaxDb" id="273057-SSO1356"/>
    <dbReference type="EnsemblBacteria" id="AAK41592">
        <property type="protein sequence ID" value="AAK41592"/>
        <property type="gene ID" value="SSO1356"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1356"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1362"/>
    <dbReference type="eggNOG" id="arCOG03852">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1665579_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YG6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="18971" checksum="96F444D0612B9AB0" modified="2001-10-01" version="1">MEMEKEKVLNILRNSSNLPLSLIKEFLSDKDKDIKHEAWNYVILNVKDKEFLLELLSFHDTGTRYRAWNSVPEFIISGRLTLEEVISRKRYFLEMLKDDNKVVRALSWYVTLKPLLEMKIVKMEEILSYSPFLCELINSEFHDVVLDTMDEFRITCKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YG8</accession>
    <name>Q97YG8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyl-hydrolase family 116 catalytic region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1353</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90291">
        <property type="entry name" value="F90291"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923346.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YG8"/>
    <dbReference type="FunCoup" id="Q97YG8">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1353"/>
    <dbReference type="CAZy" id="GH116">
        <property type="family name" value="Glycoside Hydrolase Family 116"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1353"/>
    <dbReference type="DNASU" id="1454368"/>
    <dbReference type="EnsemblBacteria" id="AAK41589">
        <property type="protein sequence ID" value="AAK41589"/>
        <property type="gene ID" value="SSO1353"/>
    </dbReference>
    <dbReference type="GeneID" id="1454368"/>
    <dbReference type="KEGG" id="sso:SSO1353"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1359"/>
    <dbReference type="eggNOG" id="arCOG03865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027051_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YG8"/>
    <dbReference type="PhylomeDB" id="Q97YG8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006775">
        <property type="entry name" value="GH116_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024462">
        <property type="entry name" value="GH116_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052566">
        <property type="entry name" value="Non-lysos_glucosylceramidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654">
        <property type="entry name" value="BILE ACID BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654:SF0">
        <property type="entry name" value="NON-LYSOSOMAL GLUCOSYLCERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04685">
        <property type="entry name" value="DUF608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12215">
        <property type="entry name" value="Glyco_hydr_116N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl-hydrolase family 116 N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl-hydrolase family 116 catalytic region" evidence="1">
        <location>
            <begin position="329"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="663" mass="75908" checksum="5CB8B86BA56091C6" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YJ1</accession>
    <name>Q97YJ1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO7710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99289">
        <property type="entry name" value="A99289"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YJ1"/>
    <dbReference type="PaxDb" id="273057-SSO7710"/>
    <dbReference type="EnsemblBacteria" id="AAK41568">
        <property type="protein sequence ID" value="AAK41568"/>
        <property type="gene ID" value="SSO7710"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7710"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1333"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183488_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YJ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="96" mass="11560" checksum="0F4C5D5B6F35AF51" modified="2001-10-01" version="1">MDDVVVGKDDVKYVRIPTRLAEAHHWKSLAESLQRKYSRRWRSNREILLRIRSFHLKARRIMEDFARKVGKWVVDEAKRMGANVVKLERLTNMIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YK8</accession>
    <name>Q97YK8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Repressor in ring oxydation complex/ phenylacetic acid degradation pathway related protein (PaaX)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">paaX</name>
        <name evidence="4" type="ordered locus">SSO1315</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90286">
        <property type="entry name" value="H90286"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923308.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YK8"/>
    <dbReference type="STRING" id="273057.SSO1315"/>
    <dbReference type="PaxDb" id="273057-SSO1315"/>
    <dbReference type="EnsemblBacteria" id="AAK41551">
        <property type="protein sequence ID" value="AAK41551"/>
        <property type="gene ID" value="SSO1315"/>
    </dbReference>
    <dbReference type="GeneID" id="1454330"/>
    <dbReference type="KEGG" id="sso:SSO1315"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1316"/>
    <dbReference type="eggNOG" id="arCOG07233">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067515_2_0_2"/>
    <dbReference type="InParanoid" id="Q97YK8"/>
    <dbReference type="PhylomeDB" id="Q97YK8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048846">
        <property type="entry name" value="PaaX-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012906">
        <property type="entry name" value="PaaX-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013225">
        <property type="entry name" value="PaaX_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011965">
        <property type="entry name" value="PaaX_trns_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30319">
        <property type="entry name" value="PHENYLACETIC ACID REGULATOR-RELATED TRANSCRIPTIONAL REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30319:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PAAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07848">
        <property type="entry name" value="PaaX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08223">
        <property type="entry name" value="PaaX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20803">
        <property type="entry name" value="PaaX_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF020623">
        <property type="entry name" value="PaaX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcriptional repressor PaaX-like N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Transcriptional repressor PaaX-like central Cas2-like" evidence="3">
        <location>
            <begin position="89"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Transcriptional repressor PaaX-like C-terminal" evidence="2">
        <location>
            <begin position="175"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="266" mass="31577" checksum="F156F2E54456C25B" modified="2001-10-01" version="1">MKIQSLFFTLYGDYIKDAGGTISSKSLIIILKEFGFSEGAIRAGLHRMKKAGLIVSERGKDKKIRYKLSEKGLLRLLEGTRRVYEKTRRRWDGKWRIVVYNIPENNREVRDRLRRELKWLGFGMLAQSTWISPNPIEDTLRKFINDLYNSTNSVKVDIFVADYLDQPNHLVERCWNLVEVEQAYKSFLEEWSPMLKKVNSMKSNEAFVTRIELVHEYRKFLNIDPDLPEDLLPQNWIGYKAYDLFMKLREELTPKANEFFYKVYEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="142" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YP3</accession>
    <name>Q97YP3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oligo/dipeptide transport, ATP binding protein. (DppD-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">dppD-2</name>
        <name evidence="5" type="ordered locus">SSO1282</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90282">
        <property type="entry name" value="H90282"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990045.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YP3"/>
    <dbReference type="FunCoup" id="Q97YP3">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1282"/>
    <dbReference type="PaxDb" id="273057-SSO1282"/>
    <dbReference type="EnsemblBacteria" id="AAK41519">
        <property type="protein sequence ID" value="AAK41519"/>
        <property type="gene ID" value="SSO1282"/>
    </dbReference>
    <dbReference type="GeneID" id="1454298"/>
    <dbReference type="KEGG" id="sso:SSO1282"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1281"/>
    <dbReference type="eggNOG" id="arCOG00181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="InParanoid" id="Q97YP3"/>
    <dbReference type="PhylomeDB" id="Q97YP3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067:SF3">
        <property type="entry name" value="MALTOSE ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067">
        <property type="entry name" value="OLIGOPEPTIDE/DIPEPTIDE ABC TRANSPORTER, ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="323" mass="36248" checksum="2E55AF497CF35F86" modified="2001-10-01" version="1">MSLQVRDLNISYKIGKGSIRAINRVYFQLEKGDSLGVIGETGSGKTTIANAIMRALPENAEYSGSIFFNNKDILKMDQETYRKEIRWKKISIIPQYSMNAFNPIKRVGEQLVKIMMENDSNIDTSKASERIFYLFNQLSLPPEIYKKLPDELSGGQRQRVVIASALLLDPELVIADEPTTALDVINQARVISLIKREIVDKQKTLIYITHDIAVVAGVAKKVLTLYAGEIMEFGDSRKIFKEPLHPYSKGLLSSVPDIRFGKVKKVHYIPGDPPDLSKEIKGCPFAPRCSIAKDICKVEKPKLRYVDGRYVACHLVGGEENGQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2018-09-12" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YU3</accession>
    <name>CS2H_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carbon disulfide hydrolase</fullName>
            <shortName evidence="3">CS(2) hydrolase</shortName>
            <ecNumber evidence="2">3.13.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1214</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Nature" volume="478" first="412" last="416">
            <title>Evolution of a new enzyme for carbon disulphide conversion by an acidothermophilic archaeon.</title>
            <authorList>
                <person name="Smeulders M.J."/>
                <person name="Barends T.R."/>
                <person name="Pol A."/>
                <person name="Scherer A."/>
                <person name="Zandvoort M.H."/>
                <person name="Udvarhelyi A."/>
                <person name="Khadem A.F."/>
                <person name="Menzel A."/>
                <person name="Hermans J."/>
                <person name="Shoeman R.L."/>
                <person name="Wessels H.J."/>
                <person name="van den Heuvel L.P."/>
                <person name="Russ L."/>
                <person name="Schlichting I."/>
                <person name="Jetten M.S."/>
                <person name="Op den Camp H.J."/>
            </authorList>
            <dbReference type="PubMed" id="22012399"/>
            <dbReference type="DOI" id="10.1038/nature10464"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>PATHWAY</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the conversion of carbon disulfide into hydrogen sulfide and carbon dioxide, with carbonyl sulfide as an intermediate. Likely plays a key role in sulfur metabolism in S.solfataricus. Does not show carbonic anhydrase activity (hydration of CO(2) to carbonate).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>carbon disulfide + 2 H2O = 2 hydrogen sulfide + CO2 + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:38143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:23012"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="EC" id="3.13.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">86 uM for carbon disulfide</KM>
            <Vmax evidence="2">96.0 nmol/min/ug enzyme towards hydrogen sulfide formation</Vmax>
        </kinetics>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sulfur metabolism; hydrogen sulfide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a hexadecameric catenane homooligomer, through interactions of two interlocked octameric rings.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="3.13.1.5" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90275">
        <property type="entry name" value="F90275"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923252.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YU3"/>
    <dbReference type="SMR" id="Q97YU3"/>
    <dbReference type="STRING" id="273057.SSO1214"/>
    <dbReference type="PaxDb" id="273057-SSO1214"/>
    <dbReference type="EnsemblBacteria" id="AAK41461">
        <property type="protein sequence ID" value="AAK41461"/>
        <property type="gene ID" value="SSO1214"/>
    </dbReference>
    <dbReference type="GeneID" id="1454240"/>
    <dbReference type="KEGG" id="sso:SSO1214"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1214"/>
    <dbReference type="eggNOG" id="arCOG02860">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084253_1_1_2"/>
    <dbReference type="InParanoid" id="Q97YU3"/>
    <dbReference type="PhylomeDB" id="Q97YU3"/>
    <dbReference type="SABIO-RK" id="Q97YU3"/>
    <dbReference type="UniPathway" id="UPA00140"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03379">
        <property type="entry name" value="beta_CA_cladeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43175:SF3">
        <property type="entry name" value="CARBON DISULFIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43175">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="beta-carbonic anhydrase, cab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000444998" description="Carbon disulfide hydrolase">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="G0WXL9"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="22012399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="22012399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="204" mass="23678" checksum="AA05BEF5269714B4" modified="2001-10-01" version="1">MISEYVDEEIKRREDYSLRRLKGIPNDRRLWILTCMDERVHVEEALGIRPEDAHIYRNAGGIVTDDAIRSASLTTNFFGTKEIIVITHTDCGMIRFTGDEVAKYFLDKGVKVNELQIDPLLPSLRLQSTEDFTKWFKFFRDLGANSPDDIALKNAEILKNHPLIPKNVTISAYVYEVETHKLRKPHQRLYELTSRFEHGTVVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YW3</accession>
    <name>Q97YW3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phytoene dehydrogenase related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90272">
        <property type="entry name" value="F90272"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992797.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YW3"/>
    <dbReference type="STRING" id="273057.SSO1188"/>
    <dbReference type="PaxDb" id="273057-SSO1188"/>
    <dbReference type="EnsemblBacteria" id="AAK41437">
        <property type="protein sequence ID" value="AAK41437"/>
        <property type="gene ID" value="SSO1188"/>
    </dbReference>
    <dbReference type="GeneID" id="1454216"/>
    <dbReference type="KEGG" id="sso:SSO1188"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1187"/>
    <dbReference type="eggNOG" id="arCOG01521">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_651528_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YW3"/>
    <dbReference type="PhylomeDB" id="Q97YW3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10668">
        <property type="entry name" value="PHYTOENE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10668:SF103">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="430" mass="49142" checksum="0810096D96C5A872" modified="2001-10-01" version="1">MRYDVIIIGAGHNGLVSSIYLAQKGLKVLVIEARDRPGGMADTAEYKGVKYSRASYVLGLFPKRIQEELGIVFPTIDSDIADVFVTEEGKVVNIWRNKEKRLEELKRLGQYKYPKMEELLFKIKEKIEQEMQYVIKPPSFEDFKKAVEGTELEIFTQPSRKFLNEYLDEEFQPYFSYGFMYDLPAYVVAYYFSLDWKIVKGGMGKVGEMLMSRARQLGVDFLFNTKISEIVIKDNVATGVRTDNDKIVESKIVINAASPVLLNKLTNGLLKVHHPEFRPRWKRDTLVLRELPNLPDYMRSHLDTLFTLPIGEVTIPSAVDNSLGGHVMTIMGSYEEAKEFFPDLEKKVIYIDRLDAYKLEREYNAPYGDMNHMPMYTEYLFDGRPVKGWGYATPIKNLYITGSGTYPGGQVTGVPGRNAAMKIMTDLGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YW9</accession>
    <name>Q97YW9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Second ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1182</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90271">
        <property type="entry name" value="H90271"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923233.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YW9"/>
    <dbReference type="STRING" id="273057.SSO1182"/>
    <dbReference type="PaxDb" id="273057-SSO1182"/>
    <dbReference type="EnsemblBacteria" id="AAK41431">
        <property type="protein sequence ID" value="AAK41431"/>
        <property type="gene ID" value="SSO1182"/>
    </dbReference>
    <dbReference type="GeneID" id="1454210"/>
    <dbReference type="KEGG" id="sso:SSO1182"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="InParanoid" id="Q97YW9"/>
    <dbReference type="PhylomeDB" id="Q97YW9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF22">
        <property type="entry name" value="TNPB-LIKE PROTEIN MJ1635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="188"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="309"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="422" mass="50137" checksum="24C798069548524D" modified="2001-10-01" version="1">MLKNLRIGKFEPEEEYVHFTYSIKNSEREKSKELIKEYRTLLQKAIDYLWNLTKIQVRKKNGNYKITLPKKKEVYKPLREELEKINHLASHYVDKAINDAFSILKSWRKRAIKGRASIEKPRVKKAYVRIKTTLRKVVGESVRITVRPYEYITFSWSKSWFSRRVRELELGEPIIKEEKVYLSFRYKLPWVTPLNFLAIDSNLYTLDAYDGEKFVTISLKQLYSMKYSMEVKRAKVQSFASKYTKRGRELMRKYSHRERNSVLDFVRKFVNALLDLYPITFFAVEKLNKESMFKDANDSLSRKISRTVWRSIHRVLRYKAPLYGSFVKEVNPHLTSRSCPRCGFVSRKVGKTFECERCGFKLDRQLNASLNIYLKMCGFPHIRDIPRVWVGVIPLMGRRGMNVRDFGEAQGLRIDIEYHEIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YZ0</accession>
    <name>Q97YZ0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90269">
        <property type="entry name" value="C90269"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991584.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YZ0"/>
    <dbReference type="PaxDb" id="273057-SSO1160"/>
    <dbReference type="DNASU" id="1454189"/>
    <dbReference type="EnsemblBacteria" id="AAK41410">
        <property type="protein sequence ID" value="AAK41410"/>
        <property type="gene ID" value="SSO1160"/>
    </dbReference>
    <dbReference type="GeneID" id="1454189"/>
    <dbReference type="KEGG" id="sso:SSO1160"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1153"/>
    <dbReference type="eggNOG" id="arCOG07292">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754787_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YZ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43449">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43449:SF3">
        <property type="entry name" value="POLYMERASE NUCLEOTIDYL TRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="148" mass="17210" checksum="CC77FB4C65C532EF" modified="2001-10-01" version="1">MQRIILENMELFRKATFLLRREGVLAIVFFGSRVMGKYKKDSDLDVLIIVKDEAKELNFSDARLRFVKDTNIYLDTVIMTNKEFKNNLTLGTVLMGVSIAFCVTYDEINVYEELEKWSNDIRKYGAVLQLPYGSFIVGRTIRKCKISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YZ4</accession>
    <name>Q97YZ4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90268">
        <property type="entry name" value="G90268"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991589.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YZ4"/>
    <dbReference type="STRING" id="273057.SSO1155"/>
    <dbReference type="PaxDb" id="273057-SSO1155"/>
    <dbReference type="EnsemblBacteria" id="AAK41406">
        <property type="protein sequence ID" value="AAK41406"/>
        <property type="gene ID" value="SSO1155"/>
    </dbReference>
    <dbReference type="GeneID" id="1454185"/>
    <dbReference type="KEGG" id="sso:SSO1155"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1149"/>
    <dbReference type="eggNOG" id="arCOG03167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041527_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YZ4"/>
    <dbReference type="PhylomeDB" id="Q97YZ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025420">
        <property type="entry name" value="DUF4143"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295:SF19">
        <property type="entry name" value="ARCHAEAL ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13635">
        <property type="entry name" value="DUF4143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="41"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4143" evidence="2">
        <location>
            <begin position="234"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="50735" checksum="39FE75686CDFB3B2" modified="2001-10-01" version="1">MNRELILKALIDWNFWYKNQFVGIEREYSDQLLDLLEKGLAISVIGVKRSGKSTIINQVVKKLIEKKGEDPFDTLIVNFEDSRFGDIRTANDLFSLYELYKELRKKKKDSKPYIFLDEVQKIKGWEGFVRSIVDRKEANIVISGSTASVNNKSVREVLAGRHLILEVFPLSFKEFLEFKNIRLETELDMIAKESEIKVLFSEYLSYGGFPLVVSNERDKEKILLQLYEDIIFKDVISECNIRNEEEAKNLAIFYISNVGNKIRFRKISRSLNIPFRNVQRYTECMKNAYLIFFVKALSPKLSEMAKYDKKVYCIDNGISNVLGYRLNQNIGSLFENLIFLELLRRYGINNVFYYRGRRGEVDFVVKVKDEIREIYQVTYQLSDVERELKGIEEFLKIRKTKAYIITFDDEGEIKVGDDLVKVVKGWKWLLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z49</accession>
    <name>Q97Z49_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAM-dependent MTase TRM10-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1087</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90261">
        <property type="entry name" value="D90261"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989721.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z49"/>
    <dbReference type="STRING" id="273057.SSO1087"/>
    <dbReference type="PaxDb" id="273057-SSO1087"/>
    <dbReference type="DNASU" id="1454126"/>
    <dbReference type="EnsemblBacteria" id="AAK41347">
        <property type="protein sequence ID" value="AAK41347"/>
        <property type="gene ID" value="SSO1087"/>
    </dbReference>
    <dbReference type="GeneID" id="1454126"/>
    <dbReference type="KEGG" id="sso:SSO1087"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1085"/>
    <dbReference type="eggNOG" id="arCOG00967">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061952_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z49"/>
    <dbReference type="PhylomeDB" id="Q97Z49"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053623">
        <property type="entry name" value="TRM10_methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016742">
        <property type="entry name" value="tRNA_m1G_mtfrase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041071">
        <property type="entry name" value="Trm10_mtase_Thprot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018978">
        <property type="entry name" value="tRNA_m1G_mtfrase_arc_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="4">
        <location>
            <begin position="82"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="308" mass="35524" checksum="BE112E1BA6A094F2" modified="2001-10-01" version="1">MILGKAFARYLTNTLGVETLKITTLKKFFKTGYLQSIAINMLLYDYGISKKHDYGKLASVEERIKILKGKGEEITDYIFLKNGEIKIPSYIIPENPQFIIDLGNMDLLQDEEKISLEQQILVSIKTIREYLFDYNLKLAHTPDSFKLEGRNKIEILNYIPKDNAIVLNPYGDTIANEEIIRNTKFFIIGGIVDKGRRLKNATYELSRRYGYDELPQVKISLRNSTVGVPDRINSIIEILLKVIVGYNLEEAIISTQSNADKVSRLIKELNTLDKFDYNTILDLKNWLKIDDRLLKLALKKSKFRVHLP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z69</accession>
    <name>GCSH2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable glycine cleavage system H protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gcvH2</name>
        <name type="ordered locus">SSO1061</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="1">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90258">
        <property type="entry name" value="E90258"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989890.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z69"/>
    <dbReference type="SMR" id="Q97Z69"/>
    <dbReference type="FunCoup" id="Q97Z69">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1061"/>
    <dbReference type="PaxDb" id="273057-SSO1061"/>
    <dbReference type="EnsemblBacteria" id="AAK41324">
        <property type="protein sequence ID" value="AAK41324"/>
        <property type="gene ID" value="SSO1061"/>
    </dbReference>
    <dbReference type="GeneID" id="1454103"/>
    <dbReference type="KEGG" id="sso:SSO1061"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1057"/>
    <dbReference type="eggNOG" id="arCOG01303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097408_2_2_2"/>
    <dbReference type="InParanoid" id="Q97Z69"/>
    <dbReference type="PhylomeDB" id="Q97Z69"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002270">
        <property type="entry name" value="PRK01202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715:SF3">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0450">Lipoyl</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000166283" description="Probable glycine cleavage system H protein 2">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="2">
        <location>
            <begin position="32"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="1">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01066"/>
        </source>
    </evidence>
    <sequence length="148" mass="16299" checksum="813A7EA090AF0542" modified="2001-10-01" version="1">MVVESNCEIPENLYYFIEGKNTVWAKLESPDTIVVGITDLAQTMAGKIVKVRIKKKGTKVEKGRPVATMESGKWAGPVPAPVTGEVVEVNAEAEKSPIIINQDPYGKGWLVKMKMSNPEELKQLFSGQAAIQKLKELIASEKLTCKRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-10-11" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z78</accession>
    <name>NOP10_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Ribosome biogenesis protein Nop10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">nop10</name>
        <name type="ordered locus">SSO7115</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in ribosome biogenesis; more specifically in 18S rRNA pseudouridylation and in cleavage of pre-rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90257">
        <property type="entry name" value="D90257"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989170.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z78"/>
    <dbReference type="SMR" id="Q97Z78"/>
    <dbReference type="FunCoup" id="Q97Z78">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO7115"/>
    <dbReference type="PaxDb" id="273057-SSO7115"/>
    <dbReference type="EnsemblBacteria" id="AAK41315">
        <property type="protein sequence ID" value="AAK41315"/>
        <property type="gene ID" value="SSO7115"/>
    </dbReference>
    <dbReference type="GeneID" id="1454094"/>
    <dbReference type="KEGG" id="sso:SSO7115"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1047"/>
    <dbReference type="eggNOG" id="arCOG00906">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196480_1_0_2"/>
    <dbReference type="InParanoid" id="Q97Z78"/>
    <dbReference type="PhylomeDB" id="Q97Z78"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.40">
        <property type="entry name" value="H/ACA ribonucleoprotein complex, subunit Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00803">
        <property type="entry name" value="Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007264">
        <property type="entry name" value="H/ACA_rnp_Nop10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036756">
        <property type="entry name" value="H/ACA_rnp_Nop10_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023532">
        <property type="entry name" value="Nop10_arc-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009623">
        <property type="entry name" value="PRK13130.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13305:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13305">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NOP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04135">
        <property type="entry name" value="Nop10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144210">
        <property type="entry name" value="Nop10-like SnoRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <feature type="chain" id="PRO_0000149026" description="Ribosome biogenesis protein Nop10">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="56" mass="6669" checksum="0165A095186A4FA9" modified="2001-10-01" version="1">MKWKMRKCPKDNIYTFKDTCQICGSKTVIPHPSRFSPEDKYVKYRIELKKGITLNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZA4</accession>
    <name>Q97ZA4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Formate hydrogenlyase subunit 4 (HycD)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hycD</name>
        <name evidence="6" type="ordered locus">SSO1026</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B99254">
        <property type="entry name" value="B99254"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZA4"/>
    <dbReference type="STRING" id="273057.SSO1026"/>
    <dbReference type="PaxDb" id="273057-SSO1026"/>
    <dbReference type="EnsemblBacteria" id="AAK41289">
        <property type="protein sequence ID" value="AAK41289"/>
        <property type="gene ID" value="SSO1026"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1026"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1018"/>
    <dbReference type="eggNOG" id="arCOG01545">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_2_0_2"/>
    <dbReference type="InParanoid" id="Q97ZA4"/>
    <dbReference type="PhylomeDB" id="Q97ZA4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009326">
        <property type="term" value="C:formate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019645">
        <property type="term" value="P:anaerobic electron transport chain"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052561">
        <property type="entry name" value="ComplexI_Subunit1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43359">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43359:SF1">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="320" mass="35394" checksum="CB1B5314AC6B3ED7" modified="2001-10-01" version="1">MGSMAPNEAQIVVLTITQVIVVILLSPLYQGIYDRGKAIIEGRKGPSVLQPYYDIIKLLRKETVISNNSLSLFVYAPYIVFGIYLLISFVIPVVYPVPILLTPTVDFLGGALLFSLAAFIKIIASLESGSNFVALGVSRILSFTFLSEATLITVFFGVALITGTNNPYVTLDYVSQSLSHYFQLDHIFVSISFFMLWLFETGKLPVESPGLSEMGMIDDGVLYEYSGKLLAILKWGSYIKQYLLGSVLLNVFIFPWFLQVGIIGSLIDIAVMFGKWLLLILISVIINTTLAKLRLFKVQDYLAVAFLLSLLSLTLTVLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZB4</accession>
    <name>Q97ZB4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH dehydrogenase</fullName>
            <ecNumber evidence="7">1.6.99.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1010</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.99.3" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90252">
        <property type="entry name" value="H90252"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989228.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZB4"/>
    <dbReference type="FunCoup" id="Q97ZB4">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1010"/>
    <dbReference type="PaxDb" id="273057-SSO1010"/>
    <dbReference type="EnsemblBacteria" id="AAK41279">
        <property type="protein sequence ID" value="AAK41279"/>
        <property type="gene ID" value="SSO1010"/>
    </dbReference>
    <dbReference type="GeneID" id="12417994"/>
    <dbReference type="KEGG" id="sso:SSO1010"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1005"/>
    <dbReference type="eggNOG" id="arCOG01067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_815399_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZB4"/>
    <dbReference type="PhylomeDB" id="Q97ZB4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051169">
        <property type="entry name" value="NADH-Q_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42913:SF3">
        <property type="entry name" value="64 KDA MITOCHONDRIAL NADH DEHYDROGENASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42913">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="2"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="331" mass="37101" checksum="F030AFADA6D99921" modified="2001-10-01" version="1">MILILGGGFAGVSAYNQNKENSLVVDRKDYFLLTPWIIDFICGMKKLEDIIVKYKKVILGNVQKIDFKNKKVILDNSKELTYDKLIVSLGHHQNLPRLKGAKEYAHKIETLEDAIELKRRLNEVKDITIIGGGATGVELAGNIKGKKITLVQRRNRLLPTMSTASSKKAEDLLRELGVNLMLGVEAIEIKKDSVVTSYGEIKTELTIFAGGLKGPQIVGNLHANKNHRLLVDKNLKSIEYNDVYGAGDCVTFEDKEIPMTADIAVAAGRVVMKNILGNEIEFKPKRLATTIRIRNEFFGDFGENYVEGKFAKILKDISYLESLLLPRRLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZC6</accession>
    <name>Q97ZC6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nicotinate-nucleotide pyrophosphorylase [carboxylating]</fullName>
            <ecNumber evidence="6">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO0996</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>nicotinate beta-D-ribonucleotide + CO2 + diphosphate = quinolinate + 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Hexamer formed by 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the NadC/ModD family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90251">
        <property type="entry name" value="D90251"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989246.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZC6"/>
    <dbReference type="FunCoup" id="Q97ZC6">
        <property type="interactions" value="232"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0996"/>
    <dbReference type="PaxDb" id="273057-SSO0996"/>
    <dbReference type="EnsemblBacteria" id="AAK41267">
        <property type="protein sequence ID" value="AAK41267"/>
        <property type="gene ID" value="SSO0996"/>
    </dbReference>
    <dbReference type="GeneID" id="1455234"/>
    <dbReference type="KEGG" id="sso:SSO0996"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.993"/>
    <dbReference type="eggNOG" id="arCOG01482">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039622_2_0_2"/>
    <dbReference type="InParanoid" id="Q97ZC6"/>
    <dbReference type="PhylomeDB" id="Q97ZC6"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034213">
        <property type="term" value="P:quinolinate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000030">
        <property type="entry name" value="Nicotinate-nucleotide pyrophosphorylase, carboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179:SF3">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="8">
        <location>
            <begin position="22"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="7">
        <location>
            <begin position="104"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="275" mass="30848" checksum="06A3B09D6EAB4490" modified="2001-10-01" version="1">MIDWIYVKRLLDYLEEDVMPEDITTKFLEGIRARGIVRCKDSGILAGNRFIVPFLKYLGFSNINGEKDGREVKKGDIVLNFEGDADILTVERLILNFLGKLSGIATITNLMVKKAKEVNPYVRIAGTRKTTPGFRLFEKYAIEVGGGDPHRYNLSDAVLIKDNHITLYGNLEELIKRVKFSVSFTKIIEVEVSSYEDAIRAFKAGADVILLDNMKPYEILPIVNELKSKVLLEASGGITPDNVIDYAKTGVDVISSGYITHSYRSLDFSLDVEKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZE8</accession>
    <name>IF5A_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 5A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Hypusine-containing protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">eIF-5A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif5a</name>
        <name type="ordered locus">SSO0970</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Functions by promoting the formation of the first peptide bond.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-5A family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90248">
        <property type="entry name" value="E90248"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992418.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="8PUT">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="E/F/G/H=1-131"/>
    </dbReference>
    <dbReference type="PDBsum" id="8PUT"/>
    <dbReference type="AlphaFoldDB" id="Q97ZE8"/>
    <dbReference type="SMR" id="Q97ZE8"/>
    <dbReference type="FunCoup" id="Q97ZE8">
        <property type="interactions" value="197"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0970"/>
    <dbReference type="PaxDb" id="273057-SSO0970"/>
    <dbReference type="EnsemblBacteria" id="AAK41244">
        <property type="protein sequence ID" value="AAK41244"/>
        <property type="gene ID" value="SSO0970"/>
    </dbReference>
    <dbReference type="GeneID" id="1455211"/>
    <dbReference type="KEGG" id="sso:SSO0970"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.969"/>
    <dbReference type="eggNOG" id="arCOG04277">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102600_3_0_2"/>
    <dbReference type="InParanoid" id="Q97ZE8"/>
    <dbReference type="PhylomeDB" id="Q97ZE8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04467">
        <property type="entry name" value="S1_aIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000038">
        <property type="entry name" value="Translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000334">
        <property type="entry name" value="Translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00085">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048670">
        <property type="entry name" value="IF5A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
        <property type="entry name" value="Trans_elong_IF5A_hypusine_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022847">
        <property type="entry name" value="Transl_elong_IF5A_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003076">
        <property type="entry name" value="PRK03999.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21485">
        <property type="entry name" value="IF5A-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00302">
        <property type="entry name" value="IF5A_HYPUSINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0385">Hypusine</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142504" description="Translation initiation factor 5A">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="modified residue" description="Hypusine" evidence="1">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="4"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="8"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="16"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="34"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="49"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="54"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="64"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="73"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="84"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="90"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="93"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="104"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="115"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="124"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="8PUT"/>
        </source>
    </evidence>
    <sequence length="131" mass="14492" checksum="D0C5EB9A85E1B5F5" modified="2001-10-01" version="1">MSITYTTVGELKVGSYVVIDGEPCRVVEVTKAKTGKHGSAKANVVAIGVFSGAKKTLMAPVDQQVEVPIIEKHIGQIIADMGNKIQVMDLESYETFEIEKPTEDELASKIKPNAELEYWEIMGRRKIVRVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2026-01-28" version="136" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZF0</accession>
    <name>TOP6B_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type 2 DNA topoisomerase 6 subunit B</fullName>
            <ecNumber evidence="1">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Type II DNA topoisomerase VI subunit B</fullName>
            <shortName evidence="1">TopoVI-B</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">top6B</name>
        <name type="ordered locus">SSO0968</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Heterotetramer of two Top6A and two Top6B chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TOP6B family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90248">
        <property type="entry name" value="C90248"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992415.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZF0"/>
    <dbReference type="SMR" id="Q97ZF0"/>
    <dbReference type="FunCoup" id="Q97ZF0">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0968"/>
    <dbReference type="PaxDb" id="273057-SSO0968"/>
    <dbReference type="EnsemblBacteria" id="AAK41242">
        <property type="protein sequence ID" value="AAK41242"/>
        <property type="gene ID" value="SSO0968"/>
    </dbReference>
    <dbReference type="GeneID" id="1455209"/>
    <dbReference type="KEGG" id="sso:SSO0968"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.967"/>
    <dbReference type="eggNOG" id="arCOG01165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006403_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZF0"/>
    <dbReference type="PhylomeDB" id="Q97ZF0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16933">
        <property type="entry name" value="HATPase_TopVIB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00823">
        <property type="entry name" value="TopoIIB_Trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.50:FF:000014">
        <property type="entry name" value="Type 2 DNA topoisomerase 6 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000091">
        <property type="entry name" value="Type 2 DNA topoisomerase 6 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000062">
        <property type="entry name" value="Type 2 DNA topoisomerase 6 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00322">
        <property type="entry name" value="Top6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005734">
        <property type="entry name" value="TopoVI_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015320">
        <property type="entry name" value="TopoVI_B_transducer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003218">
        <property type="entry name" value="PRK04184.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01052">
        <property type="entry name" value="top6b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444:SF1">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09239">
        <property type="entry name" value="Topo-VIb_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006553">
        <property type="entry name" value="TopoVI_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0799">Topoisomerase</keyword>
    <feature type="chain" id="PRO_0000145471" description="Type 2 DNA topoisomerase 6 subunit B">
        <location>
            <begin position="1"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="97"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="106"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00322"/>
        </source>
    </evidence>
    <sequence length="530" mass="60578" checksum="87105FDC9AEF5B0E" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZF8</accession>
    <name>Q97ZF8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90247">
        <property type="entry name" value="C90247"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZF8"/>
    <dbReference type="PaxDb" id="273057-SSO0960"/>
    <dbReference type="EnsemblBacteria" id="AAK41234">
        <property type="protein sequence ID" value="AAK41234"/>
        <property type="gene ID" value="SSO0960"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0960"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.957"/>
    <dbReference type="eggNOG" id="arCOG05979">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691538_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZF8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="171" mass="19299" checksum="AC83D2BB558B3080" modified="2001-10-01" version="1">MLNMPYVDVSSKICRPNEAREIKEGDIILVYPATLNVNGKIVTFPPLSLVSDQCTHEIKKLSWIEGIILTREVFHNTAFLKCENYIEGEIEILEPTLLTAFTFKQAVGRKIKGYISKGIKGVPLLKVNDQPIVSIDKGRVNVGLCFLDNRDVLVRLFGYSIFYYITSTLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZJ1</accession>
    <name>Q97ZJ1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromatin protein Cren7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90242">
        <property type="entry name" value="F90242"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992346.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZJ1"/>
    <dbReference type="STRING" id="273057.SSO0915"/>
    <dbReference type="PaxDb" id="273057-SSO0915"/>
    <dbReference type="EnsemblBacteria" id="AAK41197">
        <property type="protein sequence ID" value="AAK41197"/>
        <property type="gene ID" value="SSO0915"/>
    </dbReference>
    <dbReference type="GeneID" id="1455164"/>
    <dbReference type="KEGG" id="sso:SSO0915"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.916"/>
    <dbReference type="eggNOG" id="arCOG04323">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2177997_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZJ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12000" checksum="7FEA1C93984F1613" modified="2001-10-01" version="1">MPKKKQIDPYVCPNCGTKAEKAQKTWQLVSPLPDSYGRITITVMGSFVCPNCGHKWKSVVSKIKAGGSSVEIEGKKGVKKIESKDSEEKANEGEVIELDLSDLDEDEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZN4</accession>
    <name>Q97ZN4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISC1190</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0850</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90236">
        <property type="entry name" value="A90236"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923139.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZN4"/>
    <dbReference type="PaxDb" id="273057-SSO0850"/>
    <dbReference type="EnsemblBacteria" id="AAK41144">
        <property type="protein sequence ID" value="AAK41144"/>
        <property type="gene ID" value="SSO0850"/>
    </dbReference>
    <dbReference type="GeneID" id="1455111"/>
    <dbReference type="KEGG" id="sso:SSO0850"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.866"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066381_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZN4"/>
    <dbReference type="PhylomeDB" id="Q97ZN4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transp_IS110-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047650">
        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS110-like N-terminal" evidence="1">
        <location>
            <begin position="6"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="2">
        <location>
            <begin position="194"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="316" mass="35835" checksum="9B7B0CA5DEF391EC" modified="2001-10-01" version="1">MEAPVAGIDVSKDKLIVYFQGKFYEFPNDKQGFEEVKKVLPRGCKVGIESTGVYHVNLAKCLMNEYEVRVINPLVIKKFKDFRGKKSDKNDAKKLAELVVNMGSEFTLSDARELTSQWDFIVKSIVRVKNRLRRDLILLGYSDSLSRKNLSEVLEGGDNIILSEVKFLLDELERLEARKREVEKRLEDVVPRDSLIFTIPGIGKTLGLIILARVGDVRRFDDKRKFVAYCGLDPVVESSGGSVVSRGISRRGDAVLRRAFYLAALTAIKVNPVIKRFYEEHKGRLKGKKLIVACARKLAVITWAVLYYNKPFDASE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-26" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q980C3</accession>
    <name>TRUB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Probable tRNA pseudouridine synthase B</fullName>
            <ecNumber>5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>tRNA pseudouridine(55) synthase</fullName>
            <shortName>Psi55 synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">truB</name>
        <name type="ordered locus">SSO0393</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pseudouridine synthase TruB family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99183">
        <property type="entry name" value="A99183"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988804.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980C3"/>
    <dbReference type="SMR" id="Q980C3"/>
    <dbReference type="FunCoup" id="Q980C3">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0393"/>
    <dbReference type="PaxDb" id="273057-SSO0393"/>
    <dbReference type="EnsemblBacteria" id="AAK40720">
        <property type="protein sequence ID" value="AAK40720"/>
        <property type="gene ID" value="SSO0393"/>
    </dbReference>
    <dbReference type="GeneID" id="1455530"/>
    <dbReference type="KEGG" id="sso:SSO0393"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.387"/>
    <dbReference type="eggNOG" id="arCOG00987">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032087_3_0_2"/>
    <dbReference type="InParanoid" id="Q980C3"/>
    <dbReference type="PhylomeDB" id="Q980C3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21148">
        <property type="entry name" value="PUA_Cbf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000043">
        <property type="entry name" value="RNA-guided pseudouridylation complex pseudouridine synthase subunit Cbf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003280">
        <property type="entry name" value="PRK04270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000121971" description="Probable tRNA pseudouridine synthase B">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="2">
        <location>
            <begin position="163"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="251" mass="28668" checksum="FA184048749B5328" modified="2001-10-01" version="1">MLPIGIENATKIMNYISKGGKEYVCVMQVHCEYNKEELAKIISSFKGEIYQRPPVRSSVKRRLRIRRIYDIEILDMDKKLVLLRVNCDSGTYMRKLCHDIGIIYGCGAHMRELRRTRSGIFTESTNLVKLHELSEAIYLYKNCKDETELRRVLIPMEFATCEIPKIVIEDSAVNALAYGAQLAVPGVVAYQNFKKNDTVAVLTLKGELVATGNALMDSEELENAKKGIVVNLSRVFMQRDIYPKAWKKHES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q980I6</accession>
    <name>Q980I6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14 15">Shikimate kinase</fullName>
            <shortName evidence="14">SK</shortName>
            <ecNumber evidence="4 14 15">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">aroK</name>
        <name evidence="18" type="ordered locus">SSO0308</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>shikimate + ATP = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the GHMP kinase family. Archaeal shikimate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="4 14 15"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90173">
        <property type="entry name" value="F90173"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980I6"/>
    <dbReference type="FunCoup" id="Q980I6">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0308"/>
    <dbReference type="PaxDb" id="273057-SSO0308"/>
    <dbReference type="EnsemblBacteria" id="AAK40645">
        <property type="protein sequence ID" value="AAK40645"/>
        <property type="gene ID" value="SSO0308"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0308"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.301"/>
    <dbReference type="eggNOG" id="arCOG01025">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073768_0_0_2"/>
    <dbReference type="InParanoid" id="Q980I6"/>
    <dbReference type="PhylomeDB" id="Q980I6"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00370">
        <property type="entry name" value="Shik_kinase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010189">
        <property type="entry name" value="SK_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01920">
        <property type="entry name" value="Shik_kin_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF3">
        <property type="entry name" value="SHIKIMATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005758">
        <property type="entry name" value="Shikimt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12 14" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="16">
        <location>
            <begin position="51"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="17">
        <location>
            <begin position="216"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="NCBIfam" id="TIGR01920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AAK40645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="275" mass="30128" checksum="228FD1A99D48C05B" modified="2001-10-01" version="1">MGEVCEIMQAYGGVSIVNALPSWYGSSMAINLKVKVEIREGKRVYSQESELIKTILNYFKEKYSIPDIEVDIESELPQKSGLKSSSAVSVALIAEIAKQYDLRNINPPILSAILSLKAGVSYTGALDDAVASYCGGIAFTYNKMFRIVKLENLEDNLSILILAKGGRQKPVNLNELRKYSHVFEEIFKIALKDYLTAMKMNGILIANILGYSLEPIEIALKKGALAAGISGNGPSYFAVSKNGEEGPIYESLKKFGDVIIVRPVSLDCKDLSIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q980L6</accession>
    <name>Q980L6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90169">
        <property type="entry name" value="G90169"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990555.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980L6"/>
    <dbReference type="STRING" id="273057.SSO0276"/>
    <dbReference type="PaxDb" id="273057-SSO0276"/>
    <dbReference type="EnsemblBacteria" id="AAK40614">
        <property type="protein sequence ID" value="AAK40614"/>
        <property type="gene ID" value="SSO0276"/>
    </dbReference>
    <dbReference type="GeneID" id="1455424"/>
    <dbReference type="KEGG" id="sso:SSO0276"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.270"/>
    <dbReference type="eggNOG" id="arCOG00999">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145829_0_0_2"/>
    <dbReference type="InParanoid" id="Q980L6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11679">
        <property type="entry name" value="archaeal_Sm_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.60">
        <property type="entry name" value="Like-Sm ribonucleoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028277">
        <property type="entry name" value="Lsm_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037156">
        <property type="entry name" value="Lsm_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14894">
        <property type="entry name" value="Lsm_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="6"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="146" mass="16514" checksum="A439248F1FF1E7E7" modified="2001-10-01" version="1">MSSGRRISSELNNLIDKTVIVKTTNGKTYTGQLYAYELSPFIISLTNVKDDSNNTFYKLMINGNSISEIIIKNPPLFEPREFAELVEKSLNLRPADIKVYDETSVVTILEKIKVSQNGVEGSGPMAQRIYDLYNDYINKKKKELGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-07-05" modified="2026-01-28" version="120" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UWX4</accession>
    <name>PDXT_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase subunit PdxT</fullName>
            <ecNumber evidence="1">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pdx2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase glutaminase subunit</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdxT</name>
        <name type="ordered locus">SSO0571</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90203">
        <property type="entry name" value="H90203"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991085.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UWX4"/>
    <dbReference type="SMR" id="Q9UWX4"/>
    <dbReference type="FunCoup" id="Q9UWX4">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0571"/>
    <dbReference type="PaxDb" id="273057-SSO0571"/>
    <dbReference type="EnsemblBacteria" id="AAK40887">
        <property type="protein sequence ID" value="AAK40887"/>
        <property type="gene ID" value="SSO0571"/>
    </dbReference>
    <dbReference type="GeneID" id="1454854"/>
    <dbReference type="GeneID" id="44129576"/>
    <dbReference type="KEGG" id="sso:SSO0571"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.580"/>
    <dbReference type="eggNOG" id="arCOG00034">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069674_2_0_2"/>
    <dbReference type="InParanoid" id="Q9UWX4"/>
    <dbReference type="PhylomeDB" id="Q9UWX4"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903600">
        <property type="term" value="C:glutaminase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:L-glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008614">
        <property type="term" value="P:pyridoxine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000041">
        <property type="entry name" value="Glutamine amidotransferase subunit pdxT, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135692" description="Pyridoxal 5'-phosphate synthase subunit PdxT">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="52"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="145"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01615"/>
        </source>
    </evidence>
    <sequence length="200" mass="21693" checksum="C0C53CC0D1172045" modified="2000-05-01" version="1">MKIGIIAYQGSFEEHFLQLKRAFDKLSLNGEIISIKIPKDLKGVDGVIIPGGESTTIGLVAKRLGLLDELKEKITSGLPVLGTCAGAIMLAKEVSDAKVGKTSQPLIGTMNISVIRNYYGRQKESFEAIVDLSKIGKDKAHVVFIRAPAIAKVWGKAQSLAELNGVTVFAEENNMLATTFHPELSDTTSIHEYFLHLVKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-23" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UWY6</accession>
    <name>DGGGP_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Digeranylgeranylglyceryl phosphate synthase</fullName>
            <shortName>DGGGP synthase</shortName>
            <shortName>DGGGPS</shortName>
            <ecNumber>2.5.1.42</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>(S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Geranylgeranylglycerol-phosphate geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ubiA-2</name>
        <name type="ordered locus">SSO0583</name>
        <name type="ORF">C21_016</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2004" name="J. Biol. Chem." volume="279" first="50197" last="50203">
            <title>(S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase from the thermoacidophilic archaeon Sulfolobus solfataricus. Molecular cloning and characterization of a membrane-intrinsic prenyltransferase involved in the biosynthesis of archaeal ether-linked membrane lipids.</title>
            <authorList>
                <person name="Hemmi H."/>
                <person name="Shibuya K."/>
                <person name="Takahashi Y."/>
                <person name="Nakayama T."/>
                <person name="Nishino T."/>
            </authorList>
            <dbReference type="PubMed" id="15356000"/>
            <dbReference type="DOI" id="10.1074/jbc.M409207200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>COFACTOR</scope>
        <scope>PH DEPENDENCE</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C2 hydroxyl of (S)-3-O-geranylgeranylglyceryl phosphate (GGGP). This reaction is the second ether-bond-formation step in the biosynthesis of archaeal membrane lipids. Cannot use other prenyl donors, i.e. farnesyl diphosphate (FPP) and phytyl diphosphate. Moreover, 4-hydroxybenzoate, 1,4-dihydroxy 2-naphthoate, homogentisate, and alpha-glycerophosphate do not function as prenyl acceptor substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>sn-3-O-(geranylgeranyl)glycerol 1-phosphate + (2E,6E,10E)-geranylgeranyl diphosphate = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18109"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="EC" id="2.5.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="2">Magnesium. Can also use Ca(2+), but less efficiently.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Inhibited by EDTA in vitro.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="2">Optimum pH is 6.0.</text>
        </phDependence>
    </comment>
    <comment type="pathway">
        <text>Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UbiA prenyltransferase family. DGGGP synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.42"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99205">
        <property type="entry name" value="A99205"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923036.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UWY6"/>
    <dbReference type="SMR" id="Q9UWY6"/>
    <dbReference type="FunCoup" id="Q9UWY6">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0583"/>
    <dbReference type="PaxDb" id="273057-SSO0583"/>
    <dbReference type="EnsemblBacteria" id="AAK40896">
        <property type="protein sequence ID" value="AAK40896"/>
        <property type="gene ID" value="SSO0583"/>
    </dbReference>
    <dbReference type="GeneID" id="1454863"/>
    <dbReference type="KEGG" id="sso:SSO0583"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.591"/>
    <dbReference type="eggNOG" id="arCOG00476">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073311_1_1_2"/>
    <dbReference type="InParanoid" id="Q9UWY6"/>
    <dbReference type="PhylomeDB" id="Q9UWY6"/>
    <dbReference type="BRENDA" id="2.5.1.42">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047295">
        <property type="term" value="F:geranylgeranylglycerol-phosphate geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13961">
        <property type="entry name" value="PT_UbiA_DGGGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01286">
        <property type="entry name" value="DGGGP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023547">
        <property type="entry name" value="DGGGP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050475">
        <property type="entry name" value="Prenyltransferase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723:SF1">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000350691" description="Digeranylgeranylglyceryl phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15356000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="15356000"/>
        </source>
    </evidence>
    <sequence length="282" mass="30915" checksum="0E98AFDD6590B052" modified="2000-05-01" version="1">MSLKSYMQLVRIHNVIGAALGAIMGFLVSSQWYLELKGILLSALVVGLIAAGGYVINDVYDVEIDKINKPYRPIPSGKISVNKAKALSIALFIIGIALSILLNIYALVIALVTAIGLIYYAKDLKKTGFYGNLLVATTTALSIFYGGLAFFSDNWLLRIIIPTLYAFFLTLIREIVKGIEDYNGDSLNNVKTLATTLGINKSWRIAKILLVLLLIISPLPFFIGFNLIYLILLILVFIPFTILSIIQKETIEGASKARTYLKISAISGIIAFLLGSLPFFKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="148" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX04</accession>
    <name>PYRDB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit</fullName>
            <shortName>DHOD B</shortName>
            <shortName>DHODase B</shortName>
            <shortName>DHOdehase B</shortName>
            <ecNumber>1.3.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Dihydroorotate oxidase B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Orotate reductase (NADH)</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pyrD</name>
        <name type="ordered locus">SSO0610</name>
        <name type="ORF">C08_040</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of dihydroorotate to orotate with NAD(+) as electron acceptor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(S)-dihydroorotate + NAD(+) = orotate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.3.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="1">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of 2 PyrK and 2 PyrD type B subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.14"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90208">
        <property type="entry name" value="B90208"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991136.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX04"/>
    <dbReference type="SMR" id="Q9UX04"/>
    <dbReference type="FunCoup" id="Q9UX04">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0610"/>
    <dbReference type="PaxDb" id="273057-SSO0610"/>
    <dbReference type="EnsemblBacteria" id="AAK40921">
        <property type="protein sequence ID" value="AAK40921"/>
        <property type="gene ID" value="SSO0610"/>
    </dbReference>
    <dbReference type="GeneID" id="1454888"/>
    <dbReference type="GeneID" id="44129612"/>
    <dbReference type="KEGG" id="sso:SSO0610"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.618"/>
    <dbReference type="eggNOG" id="arCOG00603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042042_0_1_2"/>
    <dbReference type="InParanoid" id="Q9UX04"/>
    <dbReference type="PhylomeDB" id="Q9UX04"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00945"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004589">
        <property type="term" value="F:dihydroorotate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04740">
        <property type="entry name" value="DHOD_1B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000027">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00224">
        <property type="entry name" value="DHO_dh_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050074">
        <property type="entry name" value="DHO_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033888">
        <property type="entry name" value="DHOD_1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053488">
        <property type="entry name" value="DHODH_Type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024920">
        <property type="entry name" value="Dihydroorotate_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041011">
        <property type="entry name" value="dihydoor_dh_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109:SF1">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (FUMARATE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148417" description="Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit">
        <location>
            <begin position="1"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="42"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="67"/>
            <end position="71"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="178"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="229"/>
            <end position="230"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="251"/>
            <end position="252"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="290" mass="31154" checksum="65AA1512509A78CC" modified="2000-05-01" version="1">MITIKNITLNDPLIIASGIIPDVPNYVETICEKYKPSAITTKTVTLNPLEPHKPPTVIKLHDGIYMNAIGLGNPGAKAINEIGVLCPLIVSVGGSSINEIKEVVEVVQSKAKIIEINVSSPNRKGYGESLSKLIGDIIESVKSVTKLPVFVKLGPWDNVIELAGKALEKGADGLTLINTIKGLIIDVETFKPILYYGTGGVSGRCLYPIALRIIKDVYEEYGVDIIGVGGVYDWTDVIGMLAAGAKLVGLGTVLIEKGFSVIEEIRKGLQSYLLEKGLKFEDIIGISVRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX83</accession>
    <accession>Q53VM8</accession>
    <name>KADA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Adenylate kinase</fullName>
            <shortName>AK</shortName>
            <ecNumber>2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">adkA</name>
        <name type="ordered locus">SSO0694</name>
        <name type="ORF">C10_036</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Biosci. Biotechnol. Biochem." volume="66" first="2112" last="2124">
            <title>Novel trimeric adenylate kinase from an extremely thermoacidophilic archaeon, Sulfolobus solfataricus: molecular cloning, nucleotide sequencing, expression in Escherichia coli, and characterization of the recombinant enzyme.</title>
            <authorList>
                <person name="Okajima T."/>
                <person name="Kitaguchi D."/>
                <person name="Fujii K."/>
                <person name="Matsuoka H."/>
                <person name="Goto S."/>
                <person name="Uchiyama S."/>
                <person name="Kobayashi Y."/>
                <person name="Tanizawa K."/>
            </authorList>
            <dbReference type="PubMed" id="12450122"/>
            <dbReference type="DOI" id="10.1271/bbb.66.2112"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 1-14; 34-47; 65-84; 119-127; 136-147 AND 175-192</scope>
        <scope>SUBUNIT STRUCTURE</scope>
        <scope>CHARACTERIZATION</scope>
        <source>
            <strain>ATCC 35091 / DSM 1616 / IFO 15331 / JCM 8930 / P1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal adenylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3"/>
    <dbReference type="EMBL" id="AB006440">
        <property type="protein sequence ID" value="BAD95988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90217">
        <property type="entry name" value="D90217"/>
    </dbReference>
    <dbReference type="PIR" id="JC7902">
        <property type="entry name" value="JC7902"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991247.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX83"/>
    <dbReference type="SMR" id="Q9UX83"/>
    <dbReference type="FunCoup" id="Q9UX83">
        <property type="interactions" value="114"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0694"/>
    <dbReference type="PaxDb" id="273057-SSO0694"/>
    <dbReference type="EnsemblBacteria" id="AAK40995">
        <property type="protein sequence ID" value="AAK40995"/>
        <property type="gene ID" value="SSO0694"/>
    </dbReference>
    <dbReference type="GeneID" id="1454962"/>
    <dbReference type="KEGG" id="sso:SSO0694"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.694"/>
    <dbReference type="eggNOG" id="arCOG01039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119371_0_0_2"/>
    <dbReference type="InParanoid" id="Q9UX83"/>
    <dbReference type="PhylomeDB" id="Q9UX83"/>
    <dbReference type="BRENDA" id="2.7.4.3">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00234">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023477">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003122">
        <property type="entry name" value="PRK04040.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13207">
        <property type="entry name" value="AAA_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000131826" description="Adenylate kinase">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; BAD95988." evidence="3" ref="1">
        <original>A</original>
        <variation>R</variation>
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12450122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="195" mass="21326" checksum="4E293837D8492D3C" modified="2000-05-01" version="1">MKIGIVTGIPGVGKTTVLSFADKILTEKGISHKIVNYGDYMLNTALKEGYVKSRDEIRKLQIEKQRELQALAARRIVEDLSLLGDEGIGLIDTHAVIRTPAGYLPGLPRHVIEVLSPKVIFLLEADPKIILERQKRDSSRARTDYSDTAVINEVIQFARYSAMASAVLVGASVKVVVNQEGDPSIAASEIINSLM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX98</accession>
    <name>RS17_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein uS17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">30S ribosomal protein S17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps17</name>
        <name evidence="5" type="synonym">rps17AB</name>
        <name type="ordered locus">SSO0709</name>
        <name type="ORF">C10_021</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7 8 9" key="3">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8 9 10 11 12 13 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7 8 9 10 11 12 13 14">Binds 1 Zn(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90219">
        <property type="entry name" value="C90219"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991272.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="R=1-114"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="R=1-114"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="R=1-114"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="R=1-114"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="R=1-114"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="R=1-114"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="R=1-114"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="R=1-114"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="Q9UX98"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
    <dbReference type="EMDB" id="EMD-50716"/>
    <dbReference type="EMDB" id="EMD-50717"/>
    <dbReference type="EMDB" id="EMD-50724"/>
    <dbReference type="EMDB" id="EMD-50725"/>
    <dbReference type="EMDB" id="EMD-50727"/>
    <dbReference type="EMDB" id="EMD-50854"/>
    <dbReference type="SMR" id="Q9UX98"/>
    <dbReference type="FunCoup" id="Q9UX98">
        <property type="interactions" value="213"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0709"/>
    <dbReference type="PaxDb" id="273057-SSO0709"/>
    <dbReference type="EnsemblBacteria" id="AAK41010">
        <property type="protein sequence ID" value="AAK41010"/>
        <property type="gene ID" value="SSO0709"/>
    </dbReference>
    <dbReference type="GeneID" id="1454977"/>
    <dbReference type="KEGG" id="sso:SSO0709"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.709"/>
    <dbReference type="eggNOG" id="arCOG04096">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073626_0_3_2"/>
    <dbReference type="InParanoid" id="Q9UX98"/>
    <dbReference type="PhylomeDB" id="Q9UX98"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_A">
        <property type="entry name" value="Ribosomal_uS17_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_uS17_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019978">
        <property type="entry name" value="Ribosomal_uS17_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_uS17_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006345">
        <property type="entry name" value="PRK08572.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF9">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000128504" description="Small ribosomal subunit protein uS17">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 8 9">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8 9">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8 9">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8 9">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="CAB57594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="10">
        <source>
            <dbReference type="PDB" id="9FRL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="11">
        <source>
            <dbReference type="PDB" id="9FS6"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="12">
        <source>
            <dbReference type="PDB" id="9FS8"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="13">
        <source>
            <dbReference type="PDB" id="9FSF"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="14">
        <source>
            <dbReference type="PDB" id="9FY0"/>
        </source>
    </evidence>
    <sequence length="114" mass="12962" checksum="423808B4DAC5833C" modified="2000-05-01" version="1">MVSKGKTVKDPGIPNITIPEKVCEDEDCPYHGSLRVRGITLEGVIVKYRGTKAAVIERQYLYYDSKYKRYERRRSRIHAHVPPCINVREGDKVIIGECRPLSKSISFVVLGKVS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>P50386</accession>
    <name>TRPF_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <shortName>PRAI</shortName>
            <ecNumber>5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">trpF</name>
        <name type="ordered locus">SSO0892</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="1995-07" db="EMBL/GenBank/DDBJ databases">
            <title>The tryptophan operon in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Tutino M.L."/>
                <person name="Cubellis M."/>
                <person name="Sannia G."/>
                <person name="Marino G."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24"/>
    <dbReference type="EMBL" id="Z50014">
        <property type="protein sequence ID" value="CAA90310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90239">
        <property type="entry name" value="G90239"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992306.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P50386"/>
    <dbReference type="SMR" id="P50386"/>
    <dbReference type="FunCoup" id="P50386">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0892"/>
    <dbReference type="PaxDb" id="273057-SSO0892"/>
    <dbReference type="EnsemblBacteria" id="AAK41174">
        <property type="protein sequence ID" value="AAK41174"/>
        <property type="gene ID" value="SSO0892"/>
    </dbReference>
    <dbReference type="GeneID" id="1455141"/>
    <dbReference type="KEGG" id="sso:SSO0892"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.895"/>
    <dbReference type="eggNOG" id="arCOG01983">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076364_3_0_2"/>
    <dbReference type="InParanoid" id="P50386"/>
    <dbReference type="PhylomeDB" id="P50386"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894:SF1">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000154415" description="N-(5'-phosphoribosyl)anthranilate isomerase">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="204" mass="23342" checksum="0697F1FF5D2213BD" modified="1996-10-01" version="1">MVKLKICGNATLSDIIEFSKLDVDYLGIITDVVSQRFVKSEFLTFVKRYVEKPIVNVKVNVQISEIERELLVSDYFQIHRVLDDSELELLKSYDFRKRIILYVPASFEYKKYLERAIDTVDMVLVDSVKKGVGVDYNVVSSFLKDYPYLGVGGKISIDNISNFIDLNPAWLDISSSIEIYPGKKDINMVKKIVEVVKYGSSSNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>P95988</accession>
    <name>RS11_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Small ribosomal subunit protein uS11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">30S ribosomal protein S11</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">SSU ribosomal protein S11AB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps11</name>
        <name evidence="5" type="synonym">rps11AB</name>
        <name type="ordered locus">SSO0072</name>
        <name type="ORF">C04_050</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="3">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF RIBOSOMAL 30S INITIATION COMPLEX</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>RRNA-BINDING</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located on the platform of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Component of the small ribosomal subunit (PubMed:39753558). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:39753558). Located near the mRNA exit channel, it binds 16S (small) rRNA (PubMed:39753558).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S small ribosomal subunit. In inititation complexes on leaderless mRNA, it interacts with eS1 and eS26 (PubMed:39753558).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75416">
        <property type="entry name" value="S75416"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988884.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="9FHL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="M=1-132"/>
    </dbReference>
    <dbReference type="PDB" id="9FRA">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="M=1-132"/>
    </dbReference>
    <dbReference type="PDB" id="9FRK">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="M=1-132"/>
    </dbReference>
    <dbReference type="PDB" id="9FRL">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="M=1-132"/>
    </dbReference>
    <dbReference type="PDB" id="9FS6">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="M=1-132"/>
    </dbReference>
    <dbReference type="PDB" id="9FS8">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="M=1-132"/>
    </dbReference>
    <dbReference type="PDB" id="9FSF">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="M=1-132"/>
    </dbReference>
    <dbReference type="PDB" id="9FY0">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="M=1-132"/>
    </dbReference>
    <dbReference type="PDBsum" id="9FHL"/>
    <dbReference type="PDBsum" id="9FRA"/>
    <dbReference type="PDBsum" id="9FRK"/>
    <dbReference type="PDBsum" id="9FRL"/>
    <dbReference type="PDBsum" id="9FS6"/>
    <dbReference type="PDBsum" id="9FS8"/>
    <dbReference type="PDBsum" id="9FSF"/>
    <dbReference type="PDBsum" id="9FY0"/>
    <dbReference type="AlphaFoldDB" id="P95988"/>
    <dbReference type="EMDB" id="EMD-50445"/>
    <dbReference type="EMDB" id="EMD-50709"/>
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        <property type="gene ID" value="SSO0072"/>
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    <dbReference type="PATRIC" id="fig|273057.12.peg.73"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072439_6_1_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="F:rRNA binding"/>
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        <property type="entry name" value="Ribosomal protein S11"/>
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    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
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        <property type="entry name" value="Ribosomal_uS11"/>
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        <property type="entry name" value="arch_S11P"/>
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    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
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    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
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        <property type="entry name" value="RIBOSOMAL_S11"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000123283" description="Small ribosomal subunit protein uS11">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="9FHL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="9FRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="9FRK"/>
        </source>
    </evidence>
    <sequence length="132" mass="14041" checksum="952B9E3999A1DEC3" modified="1997-11-01" version="2">MSSRREIRWGIAHIYASQNNTLLTISDLTGAEIISRASGGMVVKADREKSSPYAAMLAANKAASDALEKGIMALHIKVRAPGGYGSKTPGPGAQPAIRALARAGFIIGRIEDVTPIPHDTIRRPGGRRGRRV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2026-01-28" version="157" xmlns="https://uniprot.org/uniprot">
    <accession>P95989</accession>
    <name>RPO3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo3</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1 4">DNA-directed RNA polymerase subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo3</name>
        <name evidence="1 3" type="synonym">rpoD</name>
        <name type="ordered locus">SSO0071</name>
        <name evidence="5" type="ORF">C04_051</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="8 9 10" key="3">
        <citation type="journal article" date="2008" name="Nature" volume="451" first="851" last="854">
            <title>The X-ray crystal structure of RNA polymerase from Archaea.</title>
            <authorList>
                <person name="Hirata A."/>
                <person name="Klein B.J."/>
                <person name="Murakami K.S."/>
            </authorList>
            <dbReference type="PubMed" id="18235446"/>
            <dbReference type="DOI" id="10.1038/nature06530"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.40 ANGSTROMS) OF THE RNA POLYMERASE COMPLEX IN COMPLEX WITH IRON-SULFUR (3FE-4S)</scope>
        <scope>X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS) IN COMPLEX WITH RPO11 AND IRON-SULFUR (3FE-4S)</scope>
        <scope>IRON-SULFUR-BINDING</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF CYS-183; CYS-203; CYS-206 AND CYS-209</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="1 2">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 13-subunit RNA polymerase complex.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-9022065">
            <id>P95989</id>
        </interactant>
        <interactant intactId="EBI-9022031">
            <id>Q980K0</id>
            <label>rpo11</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>2</experiments>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">The presence of the iron-sulfur cluster is required for assembly of the RNA polymerase complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2 6">X-ray crystallography in this and other archaea shows this protein binds a 3Fe-4S cluster, although a 4Fe-4S cluster has been suggested to be present in this protein.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75417">
        <property type="entry name" value="S75417"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988881.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2PA8">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.76 A"/>
        <property type="chains" value="D=1-265"/>
    </dbReference>
    <dbReference type="PDB" id="2PMZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="D/S=1-265"/>
    </dbReference>
    <dbReference type="PDB" id="3HKZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="D/O=1-265"/>
    </dbReference>
    <dbReference type="PDBsum" id="2PA8"/>
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    <dbReference type="DIP" id="DIP-60646N"/>
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        <property type="protein sequence ID" value="AAK40433"/>
        <property type="gene ID" value="SSO0071"/>
    </dbReference>
    <dbReference type="GeneID" id="1455324"/>
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    <dbReference type="PATRIC" id="fig|273057.12.peg.72"/>
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        <property type="taxonomic scope" value="Archaea"/>
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        <property type="component" value="Chromosome"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0000428">
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    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0003">3Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000132765" description="DNA-directed RNA polymerase subunit Rpo3">
        <location>
            <begin position="1"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 8 9">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 8 10">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 8 9">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Abolishes iron-sulfur-binding, causing aggregation of Rpo3 and preventing the functional Rpo3-Rpo11 subcomplex from being formed; when associated with S-203; S-206 and S-209." evidence="2">
        <original>C</original>
        <variation>S</variation>
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abolishes iron-sulfur-binding, causing aggregation of Rpo3 and preventing the functional Rpo3-Rpo11 subcomplex from being formed; when associated with S-183; S-206 and S-209." evidence="2">
        <original>C</original>
        <variation>S</variation>
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abolishes iron-sulfur-binding, causing aggregation of Rpo3 and preventing the functional Rpo3-Rpo11 subcomplex from being formed; when associated with S-183; S-203 and S-209." evidence="2">
        <original>C</original>
        <variation>S</variation>
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abolishes iron-sulfur-binding, causing aggregation of Rpo3 and preventing the functional Rpo3-Rpo11 subcomplex from being formed; when associated with S-183; S-203 and S-206." evidence="2">
        <original>C</original>
        <variation>S</variation>
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="3"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="10"/>
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        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="51"/>
            <end position="53"/>
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    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="55"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="72"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="79"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="turn" evidence="11">
        <location>
            <begin position="90"/>
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    <feature type="strand" evidence="11">
        <location>
            <begin position="93"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="103"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="107"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="111"/>
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        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="115"/>
            <end position="118"/>
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    <feature type="strand" evidence="11">
        <location>
            <begin position="129"/>
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    <feature type="strand" evidence="11">
        <location>
            <begin position="138"/>
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    <feature type="turn" evidence="11">
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            <begin position="150"/>
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        <location>
            <begin position="154"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="159"/>
            <end position="172"/>
        </location>
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        <location>
            <begin position="179"/>
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    </feature>
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        <location>
            <begin position="188"/>
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        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="194"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="200"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="208"/>
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        </location>
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    <feature type="strand" evidence="11">
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            <begin position="234"/>
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        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="238"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18235446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="11427726"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PubMed" id="18235446"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PubMed" id="8899719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <evidence type="ECO:0000305" key="7">
        <source>
            <dbReference type="PubMed" id="18235446"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="2PA8"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="10">
        <source>
            <dbReference type="PDB" id="3HKZ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="11">
        <source>
            <dbReference type="PDB" id="2PA8"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="12">
        <source>
            <dbReference type="PDB" id="2PMZ"/>
        </source>
    </evidence>
    <sequence length="265" mass="30294" checksum="C5B9B863A78250AA" modified="1997-05-01" version="1">MSINLLHKDDTRIDLVFEGYPLEFVNAIRRASMLYVPIMAVDDVYFIENNSPLYDEILAHRLALIPFMSEEALDTYRWPEECIECTENCEKCYTKIYIEAEAPNEPRMIYSKDIKSEDPSVVPISGDIPIVLLGTNQKISLEARLRLGYGKEHAKFIPVSLSVVRYYPKVEILANCEKAVNVCPEGVFELKDGKLSVKNELSCTLCEECLRYCNGSIRISFVEDKYILEIESVGSLKPERILLEAGKSIIRKIEELEKKLVEVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXH5</accession>
    <name>Q7LXH5_SACS2</name>
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        <recommendedName>
            <fullName evidence="2">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0798</name>
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    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
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    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="A90230"/>
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    <dbReference type="EnsemblBacteria" id="AAK41096">
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        <property type="gene ID" value="SSO0798"/>
    </dbReference>
    <dbReference type="GeneID" id="1455063"/>
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    <dbReference type="PATRIC" id="fig|273057.12.peg.804"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121774_5_0_2"/>
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    </dbReference>
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        <location>
            <begin position="16"/>
            <end position="118"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="130" mass="14961" checksum="2BEFAABED8B6080A" modified="2011-05-31" version="1">MKGNGFLFDASALYPLLDYIDKIDVKKIYILTLTFYEVGNAIWKEYYIHKKVKDPITLSMLFNDLLRRFNVVEDPPLDKVMKVAIDKGLTYYDASYVYVAESLGLTLVSNNRELIRKANAITLEELIKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXJ3</accession>
    <name>Q7LXJ3_SACS2</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name evidence="1" type="ordered locus">SSO0778</name>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="SSO0778"/>
    </dbReference>
    <dbReference type="GeneID" id="15297721"/>
    <dbReference type="KEGG" id="sso:SSO0778"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.779"/>
    <dbReference type="eggNOG" id="arCOG03142">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431674_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXJ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="177" mass="20041" checksum="925ACF76854B47B9" modified="2011-05-31" version="1">MKIKNVAIITDSPKVYKNILDELKRRNIQISENGEVKIVIDSVKERSDILRYVGRIIAISRGKQEFNELLIGIDTNSPYLTVVALIDGELIEYRRSYGLQPLINAISEILITYPAKRKIIGVGIGNKYGKEIYSVLSMIYENVKSIDEKYTNAKSPFNQIKDKDIRAAYSIALRASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXL0</accession>
    <name>Q7LXL0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GTP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0743</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
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    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RelA/SpoT family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90222">
        <property type="entry name" value="H90222"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXL0"/>
    <dbReference type="FunCoup" id="Q7LXL0">
        <property type="interactions" value="229"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0743"/>
    <dbReference type="PaxDb" id="273057-SSO0743"/>
    <dbReference type="EnsemblBacteria" id="AAK41039">
        <property type="protein sequence ID" value="AAK41039"/>
        <property type="gene ID" value="SSO0743"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0743"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.739"/>
    <dbReference type="eggNOG" id="arCOG00357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037276_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LXL0"/>
    <dbReference type="PhylomeDB" id="Q7LXL0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01669">
        <property type="entry name" value="TGS_MJ1332_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01899">
        <property type="entry name" value="Ygr210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000002">
        <property type="entry name" value="GTP pyrophosphokinase (RelA/SpoT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.470:FF:000001">
        <property type="entry name" value="GTP-binding protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="YGR210-like_G4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007171">
        <property type="entry name" value="PRK09602.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF1">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="YGR210-like_G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="9"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="5">
        <location>
            <begin position="326"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="408" mass="45206" checksum="C027769569227C07" modified="2011-05-31" version="1">MSTKSEHMITIGIIGKTNVGKSTFFAAATLKDVEIANRPFVTINPNEGIGYVKVKCAHTEFNVKCNPKNSICIEDYRFIPVKLVDVAGLIPGAHEGRGLGNKFLDDLRQADALIHVIDASGSTNEEGVPVEPGSRDPEEDIKFIESELDEWFYSIINKDWAKFARTSDLSGKDLVDALLSKLSGVSVNRSHIIETLKVTKLENLKLMQWTEQDLRVFAKTLRSVSKPMIIAANKSDLPQARNNIRRLKEKYKWAIPTSAASEMALRKAAKAGIISYIPGDSDFTILKPLNEKQKSALEYIRTSVLQVYGSTGVQQAINTAIFDALNMIVTYPVEDERKLTDKNGNVLPDAILLKKGSTPKDLANAIHTELAKGFLYAIDVKRKMRVGENYQLQNNDVIKIVSSTARPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXR0</accession>
    <name>Q7LXR0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Glutamate--LysW ligase ArgX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">rimK-2</name>
        <name evidence="17" type="ordered locus">SSO0645</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the ATP-dependent formation of a covalent bond between the amino group of glutamate and the gamma-carboxyl group of the C-terminal glutamate residue in LysW.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[amino-group carrier protein]-C-terminal-L-glutamate + L-glutamate + ATP = [amino-group carrier protein]-C-terminal-gamma-(L-glutamyl)-L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9693"/>
            <dbReference type="Rhea" id="RHEA-COMP:13311"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:78525"/>
            <dbReference type="ChEBI" id="CHEBI:136714"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homotetramer. Interacts with LysW.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RimK family. LysX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90211">
        <property type="entry name" value="G90211"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991181.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXR0"/>
    <dbReference type="FunCoup" id="Q7LXR0">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0645"/>
    <dbReference type="PaxDb" id="273057-SSO0645"/>
    <dbReference type="EnsemblBacteria" id="AAK40950">
        <property type="protein sequence ID" value="AAK40950"/>
        <property type="gene ID" value="SSO0645"/>
    </dbReference>
    <dbReference type="GeneID" id="1454917"/>
    <dbReference type="KEGG" id="sso:SSO0645"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.647"/>
    <dbReference type="eggNOG" id="arCOG01589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054353_2_1_2"/>
    <dbReference type="InParanoid" id="Q7LXR0"/>
    <dbReference type="PhylomeDB" id="Q7LXR0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043774">
        <property type="term" value="F:coenzyme F420-2 alpha-glutamyl ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009085">
        <property type="term" value="P:lysine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000025">
        <property type="entry name" value="Alpha-aminoadipate--LysW ligase LysX protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000058">
        <property type="entry name" value="Alpha-aminoadipate--LysW ligase LysX protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000055">
        <property type="entry name" value="Glutamate--LysW ligase ArgX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054562">
        <property type="entry name" value="LysX/ArgX_preATP_grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011870">
        <property type="entry name" value="LysX_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="Rp_bS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02144">
        <property type="entry name" value="LysX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF2">
        <property type="entry name" value="COENZYME GAMMA-F420-2:ALPHA-L-GLUTAMATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22626">
        <property type="entry name" value="LysX_preATP_grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="16">
        <location>
            <begin position="91"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00059231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00064640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AAK40950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="282" mass="31574" checksum="D6227216F1CE3610" modified="2011-05-31" version="1">MKIALVVDIIRQEEKLIIKALNERQIDYDVINVGQEPIPFNKALSKYDVGIIRPVSMYRALYSSAILEAAGVHTINSTDAIGTCGDKILTYSKLFKHGIPIPDSIIAASPDAAMKAYEQMGFPLIDKPPIGSWGRMVSLIRDVYEGKTIIEHREMLNNSALKVHIIQEYIKYKERDIRCIVIGDELLGCYARNIPPNEWRANVALGGNPTPIQLDDNIRELAVKSVKLVKGEFVSIDILEHKSKGYVINELNDVPEFKGFMLATGINVAEKLVDHIVKTYRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXV7</accession>
    <name>Q7LXV7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transposase ISC1173</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO0541</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Absolutely required for transposition of IS1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase 27 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90200">
        <property type="entry name" value="E90200"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923028.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXV7"/>
    <dbReference type="PaxDb" id="273057-SSO0541"/>
    <dbReference type="EnsemblBacteria" id="AAK40860">
        <property type="protein sequence ID" value="AAK40860"/>
        <property type="gene ID" value="SSO0541"/>
    </dbReference>
    <dbReference type="GeneID" id="34442640"/>
    <dbReference type="KEGG" id="sso:SSO0541"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.543"/>
    <dbReference type="eggNOG" id="arCOG02133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076276_0_1_2"/>
    <dbReference type="InParanoid" id="Q7LXV7"/>
    <dbReference type="PhylomeDB" id="Q7LXV7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005063">
        <property type="entry name" value="Transposase_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051354">
        <property type="entry name" value="Transposase_27_IS1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033558">
        <property type="entry name" value="transpos_IS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293:SF1">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03400">
        <property type="entry name" value="DDE_Tnp_IS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK40860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="316" mass="36825" checksum="03FDA6F378B75CE0" modified="2011-05-31" version="1">MNLVALAQLILFVLRNLNLKPRKHKPEEIALAIAAYVMGVQITKLNIPPSTLYYYTRKLGVKRRKDVRPRCPSCNSDRVIKNGSSRGKSKYKCKVCGRTFYGTASHRMSREQRERILREYTNRMSLRGISKVEGRPLTTVYSSVKRAALKAYVDLSILQAQLKAFRSKFAVLDESWTYVRVRRGPRRQNLWIWNALIDGVPFFVTGDRDYNTFRLLWLSLPKCEVHYTDDYPIYQVLYHHVVGKKYTHTVESYNSFCRSHLARLARDTKAVNRSRAMIDYSLALLNVMYPQVFTDEKTPLNLAYLSGVQYIRDHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXW5</accession>
    <name>Q7LXW5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0526</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90198">
        <property type="entry name" value="G90198"/>
    </dbReference>
    <dbReference type="PIR" id="S63530">
        <property type="entry name" value="S63530"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991050.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXW5"/>
    <dbReference type="STRING" id="273057.SSO0526"/>
    <dbReference type="PaxDb" id="273057-SSO0526"/>
    <dbReference type="EnsemblBacteria" id="AAK40846">
        <property type="protein sequence ID" value="AAK40846"/>
        <property type="gene ID" value="SSO0526"/>
    </dbReference>
    <dbReference type="GeneID" id="1454813"/>
    <dbReference type="KEGG" id="sso:SSO0526"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.525"/>
    <dbReference type="eggNOG" id="arCOG05981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1064043_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXW5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="261" mass="29894" checksum="58FC2C7492816E8D" modified="2011-05-31" version="1">MLLEAQKLIFDIFKKDKNDEIKEPFYADEYGEWLIVSHNKPLVYISNILQKSIKKAGLKNHDLYVIQYIEDEKIRNLASIKGMICGNGNIKELDLATTIKNSLSDTLTVGEIKIFKLKICGILFIFFYIDVVVRNLKETRGSVKLLFPPIGVSLYEIPYTLQSLIKSVIEKTLGVNCSVSEIDTGDGTRLKAVAECRIQQTLESIEPLKKALEYFSFSEPKISLNRSNIRQIELQIFINQLKTKALIPLIWDRFIIDSLRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXY3</accession>
    <name>Q7LXY3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">JAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0111</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75378">
        <property type="entry name" value="S75378"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXY3"/>
    <dbReference type="SMR" id="Q7LXY3"/>
    <dbReference type="STRING" id="273057.SSO0111"/>
    <dbReference type="PaxDb" id="273057-SSO0111"/>
    <dbReference type="EnsemblBacteria" id="AAK40466">
        <property type="protein sequence ID" value="AAK40466"/>
        <property type="gene ID" value="SSO0111"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0111"/>
    <dbReference type="eggNOG" id="arCOG01138">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173000_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXY3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051929">
        <property type="entry name" value="VirAsm_ModProt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34858">
        <property type="entry name" value="CYSO-CYSTEINE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34858:SF1">
        <property type="entry name" value="CYSO-CYSTEINE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="13557" checksum="05D131E448F32927" modified="2011-05-31" version="1">MDNLKEKCGIICNNTFYELKNISRTEYEFICDPSDFYTTVKGKCSDDIQAIVHTHEESCEPSYKDIMSMKIWNIPWIIISKKCIKSILYLNGSILELDIHSLLSQELYHSLMKLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXZ8</accession>
    <name>Q7LXZ8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-terminal acetyltransferase complex ard1 subunit</fullName>
            <ecNumber evidence="4">2.3.1.128</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0082</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.128" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75406">
        <property type="entry name" value="S75406"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988894.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXZ8"/>
    <dbReference type="STRING" id="273057.SSO0082"/>
    <dbReference type="PaxDb" id="273057-SSO0082"/>
    <dbReference type="DNASU" id="1453270"/>
    <dbReference type="EnsemblBacteria" id="AAK40442">
        <property type="protein sequence ID" value="AAK40442"/>
        <property type="gene ID" value="SSO0082"/>
    </dbReference>
    <dbReference type="GeneID" id="1453270"/>
    <dbReference type="KEGG" id="sso:SSO0082"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.81"/>
    <dbReference type="eggNOG" id="arCOG00833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_23_0_2"/>
    <dbReference type="InParanoid" id="Q7LXZ8"/>
    <dbReference type="PhylomeDB" id="Q7LXZ8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051556">
        <property type="entry name" value="N-term/lysine_N-AcTrnsfr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919:SF8">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="151" mass="17742" checksum="8318DDB06C2285B7" modified="2011-05-31" version="1">MVIITDATEADLDQIFQIETESFEDPYPYSLLRAYLFLANKLYLVAKQREKVVGYIIGIIQYGYRGHIVSIAVEPIYRKQGIGAKLLNEIEERFKLNGARYSYLEVNTNNLSAISFYRANGYLIMYVRKNYYGRDKHAFVMVKNLYYKYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="145" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U18</accession>
    <name>Q97U18_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ser/thr protein kinase, putative</fullName>
            <ecNumber evidence="5">2.7.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO3207</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.1.-" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90506">
        <property type="entry name" value="A90506"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991669.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U18"/>
    <dbReference type="STRING" id="273057.SSO3207"/>
    <dbReference type="PaxDb" id="273057-SSO3207"/>
    <dbReference type="EnsemblBacteria" id="AAK43304">
        <property type="protein sequence ID" value="AAK43304"/>
        <property type="gene ID" value="SSO3207"/>
    </dbReference>
    <dbReference type="GeneID" id="1453223"/>
    <dbReference type="KEGG" id="sso:SSO3207"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3310"/>
    <dbReference type="eggNOG" id="arCOG03682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_418989_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U18"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="238"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="366"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK43304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="669" mass="77369" checksum="8E0FADD7739F0888" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UC6</accession>
    <name>Q97UC6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YncE family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90492">
        <property type="entry name" value="D90492"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989115.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UC6"/>
    <dbReference type="STRING" id="273057.SSO3089"/>
    <dbReference type="PaxDb" id="273057-SSO3089"/>
    <dbReference type="EnsemblBacteria" id="AAK43195">
        <property type="protein sequence ID" value="AAK43195"/>
        <property type="gene ID" value="SSO3089"/>
    </dbReference>
    <dbReference type="GeneID" id="1453114"/>
    <dbReference type="KEGG" id="sso:SSO3089"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3197"/>
    <dbReference type="eggNOG" id="arCOG02562">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_722823_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UC6"/>
    <dbReference type="PhylomeDB" id="Q97UC6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051200">
        <property type="entry name" value="Host-pathogen_enzymatic-act"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47197:SF3">
        <property type="entry name" value="DIHYDRO-HEME D1 DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47197">
        <property type="entry name" value="PROTEIN NIRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="406" mass="43871" checksum="04679D587CC36BC9" modified="2001-10-01" version="1">MNKKQLIKALSSYQLWLIVGLIVIILIGVGAAYIMLKQSQSSSIPSSTQTTNSSVSTTTSPQYSFLVFTQKGIAEIINPSSTTPFQGYTHSINISTNVPQQVYWWVEYPFENAAQSRYYLIPVNNGTVYAIDANSLKIVKEFNFGNSIGFIGVAYSPSQQLVAVADGPSGILALINVSSLNVLWEQHFVSPVNGRTYYPCDVRWSPDGNYLIVPMRFNNSVDLIALNGSLIKVLPTAAGSQPYMVSPNMQGTMVAVEYVGNNSVGFYSLPSLKLLGILQMPNGTIPQRGVFTPNGDYYLEAPSNTNQVFVISTSTFQIVKTIDLPSFSVKGLADIELMPGGQYAYVVIHGNVQSGGIIALISVSSLTVSSYVPLSTAPAVIIPVQNQVATYLVDNVLLPPVTGLHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UG3</accession>
    <name>Q97UG3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Maltose ABC transporter, permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO3059</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90487">
        <property type="entry name" value="G90487"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UG3"/>
    <dbReference type="FunCoup" id="Q97UG3">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3059"/>
    <dbReference type="TCDB" id="3.A.1.5.8">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3059"/>
    <dbReference type="EnsemblBacteria" id="AAK43158">
        <property type="protein sequence ID" value="AAK43158"/>
        <property type="gene ID" value="SSO3059"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3059"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3156"/>
    <dbReference type="eggNOG" id="arCOG00748">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_8_0_2"/>
    <dbReference type="InParanoid" id="Q97UG3"/>
    <dbReference type="PhylomeDB" id="Q97UG3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42729">
        <property type="entry name" value="OLIGO/DIPEPTIDE TRANSPORT, PERMEASE PROTEIN (DPPC-2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42729:SF1">
        <property type="entry name" value="OLIGO_DIPEPTIDE TRANSPORT, PERMEASE PROTEIN (DPPC-2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="82"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="323" mass="35332" checksum="76EB277FA29A736B" modified="2001-10-01" version="1">MKVSKYYNRIRKALFNNKFILAGIVIILVDFAIGIVGAFWTPYPVSETFGISLPPSSAHILGTDEFGHDVLSVMMASTLTSLIVGISVGALIAIISTLIGLFGGYYGGKISGVIIDILTITALTIPGVILLVIIEAYFRAASSTINITLSYIIVVIGLAITSWAFGAKQIRAQVLSISKRDYIIASRLIGEKSWRIIFNQILPSILPLTVAQFLFGVLYGILSLITAEFWGVLPTNINNLGTMLFFISSNGAYLSNQWWWILGAIIPIMVLGAGLGILNIGIDEFIDPRLKEVKPKVIPEKIVILEEQEIIEVPMLGNYKNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UR1</accession>
    <name>Q97UR1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">2-hydroxyhepta-2, 4-diene-1,7-dioate isomerase (HpcE-3)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hpcE-3</name>
        <name evidence="4" type="ordered locus">SSO2941</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90473">
        <property type="entry name" value="F90473"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992607.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UR1"/>
    <dbReference type="STRING" id="273057.SSO2941"/>
    <dbReference type="PaxDb" id="273057-SSO2941"/>
    <dbReference type="EnsemblBacteria" id="AAK43045">
        <property type="protein sequence ID" value="AAK43045"/>
        <property type="gene ID" value="SSO2941"/>
    </dbReference>
    <dbReference type="GeneID" id="1452964"/>
    <dbReference type="KEGG" id="sso:SSO2941"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3029"/>
    <dbReference type="eggNOG" id="arCOG00235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_3_4_2"/>
    <dbReference type="InParanoid" id="Q97UR1"/>
    <dbReference type="PhylomeDB" id="Q97UR1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051121">
        <property type="entry name" value="FAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796:SF4">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="55"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="288" mass="32558" checksum="A0E5927AE83B658D" modified="2001-10-01" version="1">MVRYVSFYVGNLRKIGVVEGNYVYEISEIGKEEERGNSYRLDEINVDIPIQPSAIICTLVNSPKMVGVNDKNEAKEMVKSPKFFIKLPTVAIAHRQPIISPPDAIRPEVEIGVITRFKMKDVSRSDVKKYILGYTVFNDITYPPGLKEDSYYAMRRDPTDGNVKKLLMRGTHFRNKVRDTFAPIGPYIVTEEEIGDVNSLRMRSYYNGELVQDGYSSEFIFSIEEILSELSKIVTIPPFSVISTGSIGYTNVSDASEFNLKPIESALMVAEVEKIGRLENPIKIYKSS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2019-07-03" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UZ1</accession>
    <name>GLCS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glucose-binding protein GlcS</fullName>
            <shortName evidence="4">GBP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">glcS</name>
        <name evidence="9" type="ordered locus">SSO2847</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="J. Bacteriol." volume="181" first="4285" last="4291">
            <title>Glucose transport in the extremely thermoacidophilic Sulfolobus solfataricus involves a high-affinity membrane-integrated binding protein.</title>
            <authorList>
                <person name="Albers S.V."/>
                <person name="Elferink M.G."/>
                <person name="Charlebois R.L."/>
                <person name="Sensen C.W."/>
                <person name="Driessen A.J."/>
                <person name="Konings W.N."/>
            </authorList>
            <dbReference type="PubMed" id="10400586"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 13-43</scope>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Mol. Microbiol." volume="39" first="1494" last="1503">
            <title>Sugar transport in Sulfolobus solfataricus is mediated by two families of binding protein-dependent ABC transporters.</title>
            <authorList>
                <person name="Elferink M.G."/>
                <person name="Albers S.V."/>
                <person name="Konings W.N."/>
                <person name="Driessen A.J."/>
            </authorList>
            <dbReference type="PubMed" id="11260467"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3 7 8">Part of the ABC transporter complex GlcSTUV involved in glucose uptake (Probable). Binds glucose. Can also bind galactose and mannose (PubMed:10400586, PubMed:11260467).</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Binding of glucose is strongly inhibited by galactose and mannose.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="2">Optimum pH is 1.5.</text>
        </phDependence>
    </comment>
    <comment type="subunit">
        <text evidence="7">The complex is composed of two ATP-binding proteins (GlcV), two transmembrane proteins (GlcT and GlcU) and a solute-binding protein (GlcS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="7">Extracellular side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the bacterial solute-binding protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90462">
        <property type="entry name" value="F90462"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923998.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UZ1"/>
    <dbReference type="SMR" id="Q97UZ1"/>
    <dbReference type="FunCoup" id="Q97UZ1">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2847"/>
    <dbReference type="TCDB" id="3.A.1.1.13">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2847"/>
    <dbReference type="EnsemblBacteria" id="AAK42957">
        <property type="protein sequence ID" value="AAK42957"/>
        <property type="gene ID" value="SSO2847"/>
    </dbReference>
    <dbReference type="GeneID" id="1452876"/>
    <dbReference type="GeneID" id="27429113"/>
    <dbReference type="KEGG" id="sso:SSO2847"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2932"/>
    <dbReference type="eggNOG" id="arCOG00150">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_491464_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UZ1"/>
    <dbReference type="PhylomeDB" id="Q97UZ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050490">
        <property type="entry name" value="Bact_solute-bd_prot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054925">
        <property type="entry name" value="GlcS_GBP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040930">
        <property type="entry name" value="ABC_arch_GlcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43649">
        <property type="entry name" value="ARABINOSE-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43649:SF29">
        <property type="entry name" value="OSMOPROTECTIVE COMPOUNDS-BINDING PROTEIN GGTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0762">Sugar transport</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000447612" description="Glucose-binding protein GlcS">
        <location>
            <begin position="1"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="7">
        <location>
            <begin position="39"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="526"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="7">
        <location>
            <begin position="547"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10400586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="11260467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="10400586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="11260467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <evidence type="ECO:0000305" key="7">
        <source>
            <dbReference type="PubMed" id="10400586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="8">
        <source>
            <dbReference type="PubMed" id="11260467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="554" mass="61128" checksum="1A70B61AA280525C" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V24</accession>
    <name>Q97V24_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90458">
        <property type="entry name" value="D90458"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989036.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V24"/>
    <dbReference type="PaxDb" id="273057-SSO2812"/>
    <dbReference type="EnsemblBacteria" id="AAK42923">
        <property type="protein sequence ID" value="AAK42923"/>
        <property type="gene ID" value="SSO2812"/>
    </dbReference>
    <dbReference type="GeneID" id="1452842"/>
    <dbReference type="KEGG" id="sso:SSO2812"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2899"/>
    <dbReference type="eggNOG" id="arCOG08318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1044351_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V24"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="266" mass="29572" checksum="71E215229ABC9518" modified="2001-10-01" version="1">MKWYQILIIVVAVILVISGIVILVHNTSQQQINVTKVVFPTQAQISSILGNGWNITGLFEENYSSAVNQQYPGGSALYQEYIQRANQTITLNVIQLNSTSTTLKGIKLGKYLVSANITGNSSKFDLSNIVNLEIETLKSGNGLMPTLNPLLFPANQSVSLLEFGNAITKNYTIYFETIMYNNSYISIDIANSSNISSLFTYLLKSTGQNVTVSSINGMKYFNLSFISQYGSVYYFVGINNNDNYLIFIQSQTPKAFQFFVEISTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V48</accession>
    <name>Q97V48_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO11139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90455">
        <property type="entry name" value="B90455"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V48"/>
    <dbReference type="STRING" id="273057.SSO11139"/>
    <dbReference type="PaxDb" id="273057-SSO11139"/>
    <dbReference type="EnsemblBacteria" id="AAK42897">
        <property type="protein sequence ID" value="AAK42897"/>
        <property type="gene ID" value="SSO11139"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11139"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2911714_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V48"/>
    <dbReference type="PhylomeDB" id="Q97V48"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025471">
        <property type="entry name" value="DUF4322"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14210">
        <property type="entry name" value="DUF4322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4322" evidence="1">
        <location>
            <begin position="7"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="61" mass="7029" checksum="C6BED48BBDC6DB69" modified="2001-10-01" version="1">MPIPSLNIQQIVYKLLSMINFHGRKGEEATKTLVSTSLHNDSVEKRFQSLQRITTNSEELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V51</accession>
    <name>Q97V51_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2783</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90454">
        <property type="entry name" value="G90454"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993331.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V51"/>
    <dbReference type="STRING" id="273057.SSO2783"/>
    <dbReference type="PaxDb" id="273057-SSO2783"/>
    <dbReference type="EnsemblBacteria" id="AAK42894">
        <property type="protein sequence ID" value="AAK42894"/>
        <property type="gene ID" value="SSO2783"/>
    </dbReference>
    <dbReference type="GeneID" id="1452813"/>
    <dbReference type="KEGG" id="sso:SSO2783"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2866"/>
    <dbReference type="eggNOG" id="arCOG03706">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153003_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V51"/>
    <dbReference type="PhylomeDB" id="Q97V51"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="6"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="139" mass="15986" checksum="81B6CAACEEF76BE3" modified="2001-10-01" version="1">MDEIKYVDTNVILSLVEEDANFERARKLKGLTNLVTGEITQLELNSFYSRKLKDEIKARASTLYLLQFVNVKVIEADWNKLFRKALDLSYKLELKTLDVLQIASAFLSGSKIFITFDKEIISKREIVKKYTGVSVDDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V83</accession>
    <name>Q97V83_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2752</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90450">
        <property type="entry name" value="G90450"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990259.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V83"/>
    <dbReference type="STRING" id="273057.SSO2752"/>
    <dbReference type="PaxDb" id="273057-SSO2752"/>
    <dbReference type="EnsemblBacteria" id="AAK42862">
        <property type="protein sequence ID" value="AAK42862"/>
        <property type="gene ID" value="SSO2752"/>
    </dbReference>
    <dbReference type="GeneID" id="1452781"/>
    <dbReference type="KEGG" id="sso:SSO2752"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2831"/>
    <dbReference type="eggNOG" id="arCOG07330">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_711010_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V83"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="388" mass="44341" checksum="98AFA8114B5B3002" modified="2001-10-01" version="1">MIRPIILPPKSPFSDIKGGVGTPYVIYDSKRDKHWFLFTGWSDLKGLKREGFVAPIDRSLNVDFSGIKKILPSDFPMQVDYSNNGIRGFYNEARDEFYITSTHGNDVYFFAFDQEWKLKGYKLLIKDLKKDSGFPIRPTGAYRNMHDAFAVAPNADSSGVKLYMVRSVDDLEKVRVEDLGDIGAWARSNDVLDFTLIPRFQIFIEMNTVSKWMLQSFIGPNLDELYVTEDVNKVSFLQGSLMPLLGLDDSFVQVGHPHYTTEPDGKPKLFFVSFRDTYTTRIDTGKEGYTHEIWVEYVDTFIFDPRSYGELRGKFKGRESKWYYVPNANRLIVSVNGEAELQLKADINDEVSDSVVLKRGINIIDKPTTWIRLSSSTEIGATIKALVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VE5</accession>
    <name>Q97VE5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90442">
        <property type="entry name" value="H90442"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988601.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VE5"/>
    <dbReference type="STRING" id="273057.SSO2681"/>
    <dbReference type="PaxDb" id="273057-SSO2681"/>
    <dbReference type="EnsemblBacteria" id="AAK42799">
        <property type="protein sequence ID" value="AAK42799"/>
        <property type="gene ID" value="SSO2681"/>
    </dbReference>
    <dbReference type="GeneID" id="1452718"/>
    <dbReference type="KEGG" id="sso:SSO2681"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2763"/>
    <dbReference type="eggNOG" id="arCOG03872">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1840675_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VE5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="139" mass="14980" checksum="7567BEFDCF758376" modified="2001-10-01" version="1">MQKYRKGLENALVTVLLILVAIAAVSLISYYFFGVLRHSMITTGLSISNVQMVGGILTGDLINQGASNITSITIQVRPQNNATVISNFTNSSLNIPPGHSYAFTLAVPKAIEGNNYVITVLVKYDNGQTYATSVEVIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VG7</accession>
    <name>Q97VG7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partial transposase in ISC1173</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2659</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90440">
        <property type="entry name" value="B90440"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VG7"/>
    <dbReference type="STRING" id="273057.SSO2659"/>
    <dbReference type="PaxDb" id="273057-SSO2659"/>
    <dbReference type="EnsemblBacteria" id="AAK42777">
        <property type="protein sequence ID" value="AAK42777"/>
        <property type="gene ID" value="SSO2659"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2659"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2741"/>
    <dbReference type="eggNOG" id="arCOG02133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1830678_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VG7"/>
    <dbReference type="PhylomeDB" id="Q97VG7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051354">
        <property type="entry name" value="Transposase_27_IS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293:SF1">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="140" mass="16942" checksum="54FCE1169191B172" modified="2001-10-01" version="1">MSYTLKIFILKFYIANCFVSILNIYKLNCYYLRFHIISRFTRSKVNYTDYYCVYQVLDNRVVGKKYTYLVESHNSWCRSHLARLARDTKAVTRSMRMTEYSLALLNVIYPHIFSRGRTPLNEAYLKGVQYIRENLIEFYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VH9</accession>
    <name>Q97VH9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3501 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2644</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90438">
        <property type="entry name" value="F90438"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988552.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VH9"/>
    <dbReference type="SMR" id="Q97VH9"/>
    <dbReference type="STRING" id="273057.SSO2644"/>
    <dbReference type="PaxDb" id="273057-SSO2644"/>
    <dbReference type="EnsemblBacteria" id="AAK42765">
        <property type="protein sequence ID" value="AAK42765"/>
        <property type="gene ID" value="SSO2644"/>
    </dbReference>
    <dbReference type="GeneID" id="1452684"/>
    <dbReference type="KEGG" id="sso:SSO2644"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2727"/>
    <dbReference type="eggNOG" id="arCOG06034">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097886_1_0_2"/>
    <dbReference type="InParanoid" id="Q97VH9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021890">
        <property type="entry name" value="DUF3501"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12007">
        <property type="entry name" value="DUF3501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="22420" checksum="977BA26C6F989243" modified="2001-10-01" version="1">MVKITLQDILPWRTYEKVRMDRIRRIIEVKNKRRIELGDRLTLLFENRDTVLQQIQEMVYLDKKEKNEDILEEIKIYSTLLPCDGKIKASLYIHSYDFKDLDWVYDNLKGIYNSVFLRVGNKLIQGIPEGGRDQGREFSTVQYLTFDLEGEKSTDMEVHVIHEKYRYSTKIDKSLAEDLIREAYDICE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VM9</accession>
    <name>Q97VM9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF429 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2591</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90432">
        <property type="entry name" value="D90432"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989301.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VM9"/>
    <dbReference type="PaxDb" id="273057-SSO2591"/>
    <dbReference type="EnsemblBacteria" id="AAK42715">
        <property type="protein sequence ID" value="AAK42715"/>
        <property type="gene ID" value="SSO2591"/>
    </dbReference>
    <dbReference type="GeneID" id="1454042"/>
    <dbReference type="KEGG" id="sso:SSO2591"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2672"/>
    <dbReference type="eggNOG" id="arCOG04409">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121772_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VM9"/>
    <dbReference type="PhylomeDB" id="Q97VM9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018036">
        <property type="entry name" value="DUF429_subgr"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF024051">
        <property type="entry name" value="DUF429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19735" checksum="A2C2C2C29FCB5B4E" modified="2001-10-01" version="1">MYCGIDLAVRRKTAVAILIDNEIKIIELVTNEEIIESCKVAKITAIDSPLSYHNGFRNVDKEMIKRKLRVLPPSFMKTLVQRAIQLKEKLYNVIETHPTSSMKIVGLNWKDLHEIKDYVDAALCAMAAMAYDLGYAEEIRADDGIIYLVSKRFPFELKRKNYFEFYLKGPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VS9</accession>
    <name>Q97VS9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2531</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90425">
        <property type="entry name" value="F90425"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990977.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VS9"/>
    <dbReference type="STRING" id="273057.SSO2531"/>
    <dbReference type="PaxDb" id="273057-SSO2531"/>
    <dbReference type="EnsemblBacteria" id="AAK42661">
        <property type="protein sequence ID" value="AAK42661"/>
        <property type="gene ID" value="SSO2531"/>
    </dbReference>
    <dbReference type="GeneID" id="1453988"/>
    <dbReference type="KEGG" id="sso:SSO2531"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2608"/>
    <dbReference type="eggNOG" id="arCOG01141">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1656964_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VS9"/>
    <dbReference type="PhylomeDB" id="Q97VS9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN VPS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="11"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="190" mass="22145" checksum="D5E00BA9AF040157" modified="2001-10-01" version="1">MREISIEWDGKILILSDIHYPYCNTEEINKIMLSEKPSLTVLLGDIVVSKSEDYRNFINSLKIRKNIIYVRGDEDKFRGDFDLVKIKNNGRRFILLHGHQYFNESDEYSIAKILKKINDNIPPFLFCIFFRIMLMNFNATIILGHSHALRYFKSINCVNAGTLSNVENLYNDRGYVVIEKGNVKLVQSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W20</accession>
    <name>Q97W20_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Lactoylglutathione lyase (Glyoxalase I), putative</fullName>
            <ecNumber evidence="4">4.4.1.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2426</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylmalonyl-CoA epimerase family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.5" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90414">
        <property type="entry name" value="C90414"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989399.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W20"/>
    <dbReference type="FunCoup" id="Q97W20">
        <property type="interactions" value="255"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2426"/>
    <dbReference type="PaxDb" id="273057-SSO2426"/>
    <dbReference type="EnsemblBacteria" id="AAK42570">
        <property type="protein sequence ID" value="AAK42570"/>
        <property type="gene ID" value="SSO2426"/>
    </dbReference>
    <dbReference type="GeneID" id="1453897"/>
    <dbReference type="KEGG" id="sso:SSO2426"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2504"/>
    <dbReference type="eggNOG" id="arCOG02706">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_5_2_2"/>
    <dbReference type="InParanoid" id="Q97W20"/>
    <dbReference type="PhylomeDB" id="Q97W20"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004493">
        <property type="term" value="F:methylmalonyl-CoA epimerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046491">
        <property type="term" value="P:L-methylmalonyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07249">
        <property type="entry name" value="MMCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017515">
        <property type="entry name" value="MeMalonyl-CoA_epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051785">
        <property type="entry name" value="MMCE/EMCE_epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03081">
        <property type="entry name" value="metmalonyl_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43048">
        <property type="entry name" value="METHYLMALONYL-COA EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43048:SF3">
        <property type="entry name" value="METHYLMALONYL-COA EPIMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13669">
        <property type="entry name" value="Glyoxalase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="3">
        <location>
            <begin position="5"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="142" mass="15874" checksum="0AEB85D6533CE76A" modified="2001-10-01" version="1">MQIENLDHIGIVVGNLDEAIKFYQNTFGMKLVHYELIPERGIKVAFLVSNEKNETSIELLEPIDHNDMSNTVAKFLKNRGQGLHHLAIKVSDINRALEELTAKGLQLVDTSPRKGARGHLVAFLHPKSVMGVLLELVQAKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W38</accession>
    <name>Q97W38_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribbon-helix-helix protein CopG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO10342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99412">
        <property type="entry name" value="A99412"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989429.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W38"/>
    <dbReference type="PaxDb" id="273057-SSO10342"/>
    <dbReference type="EnsemblBacteria" id="AAK42552">
        <property type="protein sequence ID" value="AAK42552"/>
        <property type="gene ID" value="SSO10342"/>
    </dbReference>
    <dbReference type="GeneID" id="42363357"/>
    <dbReference type="KEGG" id="sso:SSO10342"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2487"/>
    <dbReference type="eggNOG" id="arCOG03845">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_206914_1_0_2"/>
    <dbReference type="InParanoid" id="Q97W38"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="1">
        <location>
            <begin position="3"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="58" mass="6889" checksum="CA751055F4425B2C" modified="2001-10-01" version="1">MRVVTFKVEEDLLELLDRYAIKYGLNRSEAIRKAIEKMVRDELSKERVPVARVEKIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W48</accession>
    <name>Q97W48_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90410">
        <property type="entry name" value="G90410"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989443.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W48"/>
    <dbReference type="STRING" id="273057.SSO2395"/>
    <dbReference type="PaxDb" id="273057-SSO2395"/>
    <dbReference type="EnsemblBacteria" id="AAK42542">
        <property type="protein sequence ID" value="AAK42542"/>
        <property type="gene ID" value="SSO2395"/>
    </dbReference>
    <dbReference type="GeneID" id="1453869"/>
    <dbReference type="KEGG" id="sso:SSO2395"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2477"/>
    <dbReference type="eggNOG" id="arCOG05953">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727317_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W48"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="151" mass="18100" checksum="74F266EF950A032F" modified="2001-10-01" version="1">MSEDEIIDLLFNTQRDIRQGITIKKENNEVLNKLNEIEKLLQYLIKNKERASQQNGSLCEQVLSSTYVILDENMGDIPRKYFIIDLNSSRKLVTFKDTIDLLNLYFSQTINEQDLDDKLPKRLVPLFRFMRRNGLIYFDYDKKRYFIVDYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="111" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W87</accession>
    <name>Q97W87_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Plant-type L-asparaginase</fullName>
            <ecNumber evidence="1">3.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">L-asparagine amidohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ansA-like</name>
        <name evidence="11" type="ordered locus">SSO2350</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-asparagine + H2O = L-aspartate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="EC" id="3.5.1.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90405">
        <property type="entry name" value="F90405"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989505.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W87"/>
    <dbReference type="SMR" id="Q97W87"/>
    <dbReference type="FunCoup" id="Q97W87">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2350"/>
    <dbReference type="MEROPS" id="T02.002"/>
    <dbReference type="PaxDb" id="273057-SSO2350"/>
    <dbReference type="EnsemblBacteria" id="AAK42501">
        <property type="protein sequence ID" value="AAK42501"/>
        <property type="gene ID" value="SSO2350"/>
    </dbReference>
    <dbReference type="GeneID" id="1453828"/>
    <dbReference type="KEGG" id="sso:SSO2350"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2434"/>
    <dbReference type="eggNOG" id="arCOG04779">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021603_1_2_2"/>
    <dbReference type="InParanoid" id="Q97W87"/>
    <dbReference type="PhylomeDB" id="Q97W87"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14950">
        <property type="entry name" value="Asparaginase_2_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.30:FF:000001">
        <property type="entry name" value="Isoaspartyl peptidase/L-asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.30">
        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF6">
        <property type="entry name" value="N(4)-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="169"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="191"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="10">
        <location>
            <begin position="140"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAK42501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="276" mass="29107" checksum="804341CF3132592E" modified="2001-10-01" version="1">MRYNLPVLVVHGGAGSWQIADQDKAKLTISEALERGYYEFRKGSAIEAVVEAIYYMEESGVFDAGKGSVRNSAGYIEMDAGIMVGNTLQVGGIMGLREGSAIKKALEILFQNRHVLMIGSNYNSSNSNNSAIYESKVSGDTVGAVALDQHGNLVAGTSTGGIKGKLPGRVGDSPIPGAGYYATSNVAVSSTGIGEIILRILPAKEVDILVSLGFTIDDALRAVVNKVTKTFGKDNIGMIGLDKYGNASAYYNTKGMARGVISSDGVKKVYVFEGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WA0</accession>
    <name>Q97WA0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2337</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99404">
        <property type="entry name" value="A99404"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WA0"/>
    <dbReference type="STRING" id="273057.SSO2337"/>
    <dbReference type="PaxDb" id="273057-SSO2337"/>
    <dbReference type="EnsemblBacteria" id="AAK42488">
        <property type="protein sequence ID" value="AAK42488"/>
        <property type="gene ID" value="SSO2337"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2337"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2422"/>
    <dbReference type="eggNOG" id="arCOG07319">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144587_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WA0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="154" mass="17969" checksum="83CC78FB30A23ED9" modified="2001-10-01" version="1">MIFVINNSLYVILYISFNNFSASNLKVYKTYCITLMSVIEKFVKNIEKLNDNEEVIMLENLWIKKITNFPINLQVIEEEDGEKLHLFVLKGAEAILLHKPTNIFLYITNLTSVELETLRYITIKKKCEEADEDFVSLAYEYISFKNKAKIGIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WH5</accession>
    <name>Q97WH5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ferric uptake regulation protein (Fur)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fur</name>
        <name evidence="1" type="ordered locus">SSO2244</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90394">
        <property type="entry name" value="E90394"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991550.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WH5"/>
    <dbReference type="STRING" id="273057.SSO2244"/>
    <dbReference type="PaxDb" id="273057-SSO2244"/>
    <dbReference type="EnsemblBacteria" id="AAK42412">
        <property type="protein sequence ID" value="AAK42412"/>
        <property type="gene ID" value="SSO2244"/>
    </dbReference>
    <dbReference type="GeneID" id="1453739"/>
    <dbReference type="KEGG" id="sso:SSO2244"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2339"/>
    <dbReference type="eggNOG" id="arCOG01868">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096072_4_4_2"/>
    <dbReference type="InParanoid" id="Q97WH5"/>
    <dbReference type="PhylomeDB" id="Q97WH5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900376">
        <property type="term" value="P:regulation of secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:001101">
        <property type="entry name" value="Ferric-uptake regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202:SF7">
        <property type="entry name" value="FERRIC UPTAKE REGULATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="ZINC UPTAKE REGULATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="136" mass="15616" checksum="593415867A83C867" modified="2001-10-01" version="1">MEVDLANLLRQRNLKVTPQRIAILKLIMRGGHYSGEQIYEELKKTEPSISLSTVYNTLETLKESGILNSFEANGITWFEINRKPHVNVFCIDSNRIIDLDIEMDNFMENLTKNGLDIKNVNIIVYADCSKLGKRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WI0</accession>
    <name>Q97WI0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90393">
        <property type="entry name" value="G90393"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923811.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WI0"/>
    <dbReference type="STRING" id="273057.SSO2237"/>
    <dbReference type="PaxDb" id="273057-SSO2237"/>
    <dbReference type="EnsemblBacteria" id="AAK42406">
        <property type="protein sequence ID" value="AAK42406"/>
        <property type="gene ID" value="SSO2237"/>
    </dbReference>
    <dbReference type="GeneID" id="1453733"/>
    <dbReference type="KEGG" id="sso:SSO2237"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2333"/>
    <dbReference type="eggNOG" id="arCOG01145">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041441_5_0_2"/>
    <dbReference type="InParanoid" id="Q97WI0"/>
    <dbReference type="PhylomeDB" id="Q97WI0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523:SF1">
        <property type="entry name" value="CALCINEURIN-LIKE PHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="325" mass="36850" checksum="D9CE267BD96D4177" modified="2001-10-01" version="1">MVSLFKRNKHSLNSSTNLRLLFTSDLHASYTVFKKFINAGKIYKVNALIIGGDIAGKVLLPIIDLGNNSYNVQEKIVTSSELNSLIERFKSEGTYYAILSKGEYEEASQNKKAQDELFKKAIIETLHEWIKIAEERLKDYKIPIFINLGNDDPEYLFDVLKESEIFKVGEGEVFDLNGYEIVSYGYVNPTPWNTFREKKEEDIYSDLSKIVSKINNYSKAIYNFHAPPYNTNLDNAPLLDENLKPIIRGGEIVYTHVGSKSIRKIIEETKPLLGLHGHIHESRGFDKINNTIVINPGSEYSSGLLHAALVVLEGEFIKAHQFILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WK0</accession>
    <name>Q97WK0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acetyl-CoA synthetase (Acetate-CoA ligase) (AcsA-8)</fullName>
            <ecNumber evidence="8">6.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">acsA-8</name>
        <name evidence="8" type="ordered locus">SSO2216</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="8"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90391">
        <property type="entry name" value="C90391"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992066.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WK0"/>
    <dbReference type="FunCoup" id="Q97WK0">
        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2216"/>
    <dbReference type="PaxDb" id="273057-SSO2216"/>
    <dbReference type="EnsemblBacteria" id="AAK42386">
        <property type="protein sequence ID" value="AAK42386"/>
        <property type="gene ID" value="SSO2216"/>
    </dbReference>
    <dbReference type="GeneID" id="1453713"/>
    <dbReference type="KEGG" id="sso:SSO2216"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2312"/>
    <dbReference type="eggNOG" id="arCOG01529">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_2"/>
    <dbReference type="InParanoid" id="Q97WK0"/>
    <dbReference type="PhylomeDB" id="Q97WK0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF232">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="7">
        <location>
            <begin position="3"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="62"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="461"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="571" mass="64643" checksum="9662C463913ACB87" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WL2</accession>
    <name>Q97WL2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Spermatogenesis-associated protein 20-like TRX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90389">
        <property type="entry name" value="G90389"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WL2"/>
    <dbReference type="FunCoup" id="Q97WL2">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2204"/>
    <dbReference type="PaxDb" id="273057-SSO2204"/>
    <dbReference type="DNASU" id="1453701"/>
    <dbReference type="EnsemblBacteria" id="AAK42374">
        <property type="protein sequence ID" value="AAK42374"/>
        <property type="gene ID" value="SSO2204"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2204"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2300"/>
    <dbReference type="eggNOG" id="arCOG02007">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014051_4_1_2"/>
    <dbReference type="InParanoid" id="Q97WL2"/>
    <dbReference type="PhylomeDB" id="Q97WL2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02955">
        <property type="entry name" value="SSP411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024705">
        <property type="entry name" value="Ssp411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004879">
        <property type="entry name" value="Ssp411-like_TRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899:SF1">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03190">
        <property type="entry name" value="Thioredox_DsbH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006402">
        <property type="entry name" value="UCP006402_thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 20-like TRX" evidence="1">
        <location>
            <begin position="18"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="658" mass="75018" checksum="AC6A2F87E61239DE" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WL8</accession>
    <name>Q97WL8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NurA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99389">
        <property type="entry name" value="A99389"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992096.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WL8"/>
    <dbReference type="PaxDb" id="273057-SSO2197"/>
    <dbReference type="EnsemblBacteria" id="AAK42368">
        <property type="protein sequence ID" value="AAK42368"/>
        <property type="gene ID" value="SSO2197"/>
    </dbReference>
    <dbReference type="GeneID" id="1453695"/>
    <dbReference type="KEGG" id="sso:SSO2197"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2292"/>
    <dbReference type="eggNOG" id="arCOG07312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_820444_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WL8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018977">
        <property type="entry name" value="NurA_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09376">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00933">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NurA" evidence="1">
        <location>
            <begin position="55"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="363" mass="42109" checksum="9B1EB42C553DFE19" modified="2001-10-01" version="1">MNTNEIIKKVNELASKDVNERGKINMAVNLIFDDIISNSIDLKIGILDEIIDSEHIACAVDGSKYEIELSDVTLILARAVKILGKKKDKKSVPSEIAEDFKIIENYYDKNVISNKSILFMLTLETSLIEKCEECDVIFIDGPIVDPPTYYEEDVEIENILSLDKLALYRSTVLRRLKGKNKLIVGIVKNFSHRILIKEFLNNYPALMKARENYIIFSIMYKYRLEKSEFNKPVYLGWISWDSLINNQIILDDLKGISKAYNRYKKVFNDSLSIYSLYYQYNTTSPIVRIDVLAEREPDFTPIKYLNIWAMQSIREVTLLNKLADELSEINSQDAKKYALLFNLARENYLDINDKLAELMMRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WP1</accession>
    <name>Q97WP1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90386">
        <property type="entry name" value="B90386"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992136.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WP1"/>
    <dbReference type="PaxDb" id="273057-SSO2170"/>
    <dbReference type="EnsemblBacteria" id="AAK42345">
        <property type="protein sequence ID" value="AAK42345"/>
        <property type="gene ID" value="SSO2170"/>
    </dbReference>
    <dbReference type="GeneID" id="15299166"/>
    <dbReference type="KEGG" id="sso:SSO2170"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2265"/>
    <dbReference type="eggNOG" id="arCOG08320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_663238_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WP1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="395" mass="45044" checksum="80581E491C8210D0" modified="2001-10-01" version="1">MGLLEVYSNPEKPEILCSLIDDKGNRKEIMLIKLQDNGVHIYKTEEHYILPPIPQIDSLIKDVIEEVAEELKVDSIVYNYGNIDTNSETLRLSKEWFDMERLALASSKHVALSSDVNSRVIVGVVRFPNNAYAATVLRSEDSFPILQIFIDMSYNPPIIKKYNELGQVVESRRENIENFEDYLKSLINEEEYTLIYREFVEYNLLPAENPIQNGKTIYAGCIFKYLIGFNVGKKPSSVKKHKLARLLRAIMYLDRISNNIGVDVIIGNPSPISYLPLSIDKLKNKVESKVTKKHGLSSIHYSGVSSDVVKDVNFTSKDILSIIPIAFIILADSKKKFEEYVERIINGPTADGLDLLDEYVRQNLSNNFIAYLANLEEVLILYNDIIQDLEDNEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WS0</accession>
    <name>Q97WS0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4898 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99372">
        <property type="entry name" value="A99372"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923752.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WS0"/>
    <dbReference type="PaxDb" id="273057-SSO2046"/>
    <dbReference type="EnsemblBacteria" id="AAK42232">
        <property type="protein sequence ID" value="AAK42232"/>
        <property type="gene ID" value="SSO2046"/>
    </dbReference>
    <dbReference type="GeneID" id="1453559"/>
    <dbReference type="KEGG" id="sso:SSO2046"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2127"/>
    <dbReference type="eggNOG" id="arCOG08429">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2299469_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WS0"/>
    <dbReference type="PhylomeDB" id="Q97WS0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032603">
        <property type="entry name" value="DUF4898"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16239">
        <property type="entry name" value="DUF4898"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="11530" checksum="A94B67C5B6C86B62" modified="2001-10-01" version="1">MVVIPIEELSEHVEDSLIKMISKSGSVKFVRFVSPSLIVDAQKFFKTFVPTAKYYVVIVPDNVKNEKVKDELISMSRNSFNFTILYSYKLMDKILIIGYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WS4</accession>
    <name>Q97WS4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Allantoin permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2042</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90371">
        <property type="entry name" value="E90371"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923748.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WS4"/>
    <dbReference type="FunCoup" id="Q97WS4">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2042"/>
    <dbReference type="PaxDb" id="273057-SSO2042"/>
    <dbReference type="EnsemblBacteria" id="AAK42228">
        <property type="protein sequence ID" value="AAK42228"/>
        <property type="gene ID" value="SSO2042"/>
    </dbReference>
    <dbReference type="GeneID" id="1453555"/>
    <dbReference type="KEGG" id="sso:SSO2042"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2123"/>
    <dbReference type="eggNOG" id="arCOG03448">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021555_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WS4"/>
    <dbReference type="PhylomeDB" id="Q97WS4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015851">
        <property type="term" value="P:nucleobase transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11485">
        <property type="entry name" value="SLC-NCS1sbd_YbbW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4160.10:FF:000017">
        <property type="entry name" value="Permease for cytosine/purines uracil thiamine allantoin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF0">
        <property type="entry name" value="PURINE-URACIL PERMEASE NCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="434"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="492" mass="54490" checksum="D9B8774F1813A729" modified="2001-10-01" version="1">MSEQEINHVNLDLTEYNQGTTVVPDSYYNPNIAPLPKNAKTWTWINYATIWAGMIHNVPAFMLAGLLTFEFGPLIALMIIAIAYFTLLIALYLNGHIGTKWGIPFPSSIRPMFGIRGARVPVIMRAISALFWFSVETYAGGLILDALISIFYPSWSTISADLLGMPLHLTISFFLFWFLNVLVLFKGMDDIKKFELIAGPLVIIILGGLMIHAITLANGLSSLFQIRGNNVSLPNIALAISTMAGFWATLVLNIPDFTRFSRSQKDQLIGQTIGLPILTLLFSFIAVGLASAVIYIYNIPSNDTINYVNPVNIMYLFTDNPYITLILGISLVIATISVNVAANIVSPVYDLISLFPKKLNTWSKSAIVSAILGLLYAPWLWYNNASSIENVINLIGAGLGSVAGVMIAHYWILGKTEIKLADLFKPNGRYWYVSGYNVNALVAMIIGFSVPVIGFLIPKLSLLYDYGWYLGLFLSIAIYLGLERKREVKMEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WU5</accession>
    <name>Q97WU5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ring oxydation complex/ phenylacetic acid degradation related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90368">
        <property type="entry name" value="F90368"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923727.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WU5"/>
    <dbReference type="STRING" id="273057.SSO2019"/>
    <dbReference type="PaxDb" id="273057-SSO2019"/>
    <dbReference type="EnsemblBacteria" id="AAK42205">
        <property type="protein sequence ID" value="AAK42205"/>
        <property type="gene ID" value="SSO2019"/>
    </dbReference>
    <dbReference type="GeneID" id="7805859"/>
    <dbReference type="KEGG" id="sso:SSO2019"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2095"/>
    <dbReference type="eggNOG" id="arCOG04786">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093768_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WU5"/>
    <dbReference type="PhylomeDB" id="Q97WU5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.10:FF:000026">
        <property type="entry name" value="Ring oxydation complex/ phenylacetic acid degradation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052703">
        <property type="entry name" value="Aromatic_CoA_ox/epox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007814">
        <property type="entry name" value="PaaA_PaaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30458:SF0">
        <property type="entry name" value="1,2-PHENYLACETYL-COA EPOXIDASE, SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30458">
        <property type="entry name" value="PHENYLACETIC ACID DEGRADATION PROTEIN PAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05138">
        <property type="entry name" value="PaaA_PaaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="234" mass="27470" checksum="0B5BAA7B859E5B5E" modified="2001-10-01" version="1">MQKFNSVRELSQDLKNTLIEIIVLRADFELGMVEQISPWLVNAPTVDDRLFTAKLVSDELNHGWQLVRLLEEFNVKDRVVKIEEARLGIHMLDISNMPLFNWEDVIAFVFLVDRAGLYQLRVIKDCSFEPLANLASSMIKEEESHLFFSKNVLKAYQNKNRLQAALNFWFPRALEMLYKVKSLNEVHLRDLNVSELISDKLIEDYVRSTNDEMRNFGFVEVNYDKNLHYNIVLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-07-11" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WX3</accession>
    <name>CMR5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR system CMR subunit Cmr5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>CRISPR type III-B/RAMP module-associated protein Cmr5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cmr5</name>
        <name type="ordered locus">SSO1988</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="Mol. Cell" volume="45" first="303" last="313">
            <title>Structure and mechanism of the CMR complex for CRISPR-mediated antiviral immunity.</title>
            <authorList>
                <person name="Zhang J."/>
                <person name="Rouillon C."/>
                <person name="Kerou M."/>
                <person name="Reeks J."/>
                <person name="Brugger K."/>
                <person name="Graham S."/>
                <person name="Reimann J."/>
                <person name="Cannone G."/>
                <person name="Liu H."/>
                <person name="Albers S.V."/>
                <person name="Naismith J.H."/>
                <person name="Spagnolo L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="22227115"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2011.12.013"/>
        </citation>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>FUNCTION IN CMR COMPLEX</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). The CMR complex degrades RNA complementary to the crRNA (target RNA) within UA dinucleotides, generating 3'-OH and 5'-phosphate ends. Activity is dependent on the 8 nt long 5' tag in the crRNA, an unpaired 3' flag on the target RNA, and is stimulated by ATP. Some cleavage of the guide crRNA can also be observed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the CMR ribonucleoprotein complex, consisting of crRNA plus Cmr1/Cmr2/Cmr3/Cmr4/Cmr5/Cmr6 at 1:1 and possibly 3 Cmr7 dimers. A Cmr2/Cmr3/Cmr7 subcomplex without crRNA can also be isolated. It does not cleave target RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR system Cmr5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90365">
        <property type="entry name" value="C90365"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992995.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WX3"/>
    <dbReference type="SMR" id="Q97WX3"/>
    <dbReference type="STRING" id="273057.SSO1988"/>
    <dbReference type="PaxDb" id="273057-SSO1988"/>
    <dbReference type="EnsemblBacteria" id="AAK42178">
        <property type="protein sequence ID" value="AAK42178"/>
        <property type="gene ID" value="SSO1988"/>
    </dbReference>
    <dbReference type="GeneID" id="1453505"/>
    <dbReference type="GeneID" id="27428314"/>
    <dbReference type="KEGG" id="sso:SSO1988"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2064"/>
    <dbReference type="eggNOG" id="arCOG02654">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691615_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WX3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09654">
        <property type="entry name" value="Cmr5_III-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.30">
        <property type="entry name" value="AF1862-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023101">
        <property type="entry name" value="AF1862-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010160">
        <property type="entry name" value="CRISPR-assoc_prot_Cmr5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01881">
        <property type="entry name" value="cas_Cmr5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158568">
        <property type="entry name" value="AF1862-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000418080" description="CRISPR system CMR subunit Cmr5">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="22227115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="156" mass="18145" checksum="F6C86AAF3786277C" modified="2001-10-01" version="1">MTEVQEEKDYKLAIEAFNLVYNALDKKAREKFRSRARDMVEEIYTSGFIPTLFYIISKAGLENNNDKLTALLNSPSSKSIDLGSSDEASYMAYLFVILYYLSERNIINKEFLINKLKNNRLELIDKLYELSPIISARIKRYLMAIKRLAEALIEVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-08-29" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WY4</accession>
    <name>Y1976_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>UPF0145 protein SSO1976</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO1976</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0145 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90363">
        <property type="entry name" value="H90363"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989373.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WY4"/>
    <dbReference type="SMR" id="Q97WY4"/>
    <dbReference type="STRING" id="273057.SSO1976"/>
    <dbReference type="PaxDb" id="273057-SSO1976"/>
    <dbReference type="EnsemblBacteria" id="AAK42167">
        <property type="protein sequence ID" value="AAK42167"/>
        <property type="gene ID" value="SSO1976"/>
    </dbReference>
    <dbReference type="GeneID" id="1453494"/>
    <dbReference type="KEGG" id="sso:SSO1976"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2053"/>
    <dbReference type="eggNOG" id="arCOG02287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117144_1_1_2"/>
    <dbReference type="InParanoid" id="Q97WY4"/>
    <dbReference type="PhylomeDB" id="Q97WY4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.70">
        <property type="entry name" value="Hypothetical protein apc22750. Chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00338">
        <property type="entry name" value="UPF0145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035439">
        <property type="entry name" value="UPF0145_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002765">
        <property type="entry name" value="UPF0145_YbjQ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068:SF2">
        <property type="entry name" value="UPF0145 PROTEIN SCO3412"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068">
        <property type="entry name" value="UPF0145 PROTEIN YBJQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01906">
        <property type="entry name" value="YbjQ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117782">
        <property type="entry name" value="YbjQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000138503" description="UPF0145 protein SSO1976">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="114" mass="12255" checksum="FD6538760DF418B1" modified="2001-08-29" version="1">MSYKEGDVLVTNGEQSPGYKIVEIKGLVVGITVRSRGLGKNIIASLRSLLGGEIKEYVELAEQARLQALQRMVDNAKALGANAVVNVRFDSNELSEAMDEIIAYGTAVVVEKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X19</accession>
    <name>Q97X19_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1933</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90359">
        <property type="entry name" value="B90359"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992187.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X19"/>
    <dbReference type="STRING" id="273057.SSO1933"/>
    <dbReference type="PaxDb" id="273057-SSO1933"/>
    <dbReference type="EnsemblBacteria" id="AAK42129">
        <property type="protein sequence ID" value="AAK42129"/>
        <property type="gene ID" value="SSO1933"/>
    </dbReference>
    <dbReference type="GeneID" id="1453456"/>
    <dbReference type="KEGG" id="sso:SSO1933"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2008"/>
    <dbReference type="eggNOG" id="arCOG05914">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978675_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X19"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="284" mass="31682" checksum="07C6CF5A4C8EA048" modified="2001-10-01" version="1">MNAKKPIILTIIVLISAFIVVLASTHPTTQESGSTTDTFHYSTLDEIHTYNISPITNFISYYVNNTNATLALSLASQIIGNGTINFISFNNTFAIIGYFKASSPLSSFAFTIINKTLVKHGFVYGQYNTLLYDYKNTSAFGFDGHYFYIVKDNASFNTTFFLLKYLYTSQKIFASQPSINVIAYGNTTYGSFQLFSSNSQLILKSTGNFSSVTKLLKYFNITIGNFTVAGKVVFKNATVVVYSINATYYNRSLFVMIGINKQDNYSIVITSHQEISLAEVLKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X29</accession>
    <name>Q97X29_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISC1229</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AAK42119.1"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X29"/>
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    <dbReference type="EnsemblBacteria" id="AAK42119">
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        <property type="gene ID" value="SSO1924"/>
    </dbReference>
    <dbReference type="GeneID" id="1453446"/>
    <dbReference type="KEGG" id="sso:SSO1924"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1999"/>
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    <dbReference type="InParanoid" id="Q97X29"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Transpos_IS110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033542">
        <property type="entry name" value="transpos_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055:SF13">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33055">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS1111A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS116/IS110/IS902 C-terminal" evidence="1">
        <location>
            <begin position="236"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="357" mass="41506" checksum="37C3991C484A1527" modified="2001-10-01" version="1">MVETLSEAKSSATGVTNPYRRTEHCDKIHEYRCDKGVGVIGIDVSKDYLITSRGRVRKYENNKEGYEEILEMKPCTIVLEPMGVYAIRPSQYFKEKGIKVLQVSPNMLSREKEFRGKKTDFYDAEKIENMVDKAKEYEYNPLRELVTLYLFLKDIEVKYKNRLERTLFLVSDNNKISKERLEKLAKGDFTQEELYQLEYTPIVVEEIKVLAKTLLETQEKLKEVRRMIEEQVPDNHVLLTIPGIGRLAAGIIIGIVGDIRRFPKPESFVAYCGLDPVVERSGKAVVSKGISKRGNKYLRSLLYFLAGRNYSRNPNLLKFYETHKDRLHGRKLYAALARKLARVVWSVWYNNKPYEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X49</accession>
    <name>Q97X49_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO1906</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90355">
        <property type="entry name" value="C90355"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X49"/>
    <dbReference type="FunCoup" id="Q97X49">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1906"/>
    <dbReference type="PaxDb" id="273057-SSO1906"/>
    <dbReference type="EnsemblBacteria" id="AAK42098">
        <property type="protein sequence ID" value="AAK42098"/>
        <property type="gene ID" value="SSO1906"/>
    </dbReference>
    <dbReference type="GeneID" id="1453425"/>
    <dbReference type="KEGG" id="sso:SSO1906"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1967"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_16_0_2"/>
    <dbReference type="InParanoid" id="Q97X49"/>
    <dbReference type="PhylomeDB" id="Q97X49"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052962">
        <property type="entry name" value="AA_Transporter_AGT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547:SF1">
        <property type="entry name" value="ASPARTATE-PROTON SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="427"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="454"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="482"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="516"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="547"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="580"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="616" mass="67722" checksum="0C37831E17D05DAD" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X57</accession>
    <name>Q97X57_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1898</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
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        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="A90354"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1898"/>
    <dbReference type="EnsemblBacteria" id="AAK42088">
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        <property type="gene ID" value="SSO1898"/>
    </dbReference>
    <dbReference type="GeneID" id="1453415"/>
    <dbReference type="KEGG" id="sso:SSO1898"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1953"/>
    <dbReference type="eggNOG" id="arCOG02703">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129117_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X57"/>
    <dbReference type="PhylomeDB" id="Q97X57"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Diverse_substrate_MTase"/>
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        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942">
        <property type="entry name" value="METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942:SF4">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="46"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="190" mass="21813" checksum="93053F7B101D77EE" modified="2001-10-01" version="1">MQIIMSENDRYINPLILDNPLRFLVSSPNKIISRFSEYLKEDYTIVDLGCGPGFFTTVLARMVKTVYAIDPDERAISKLKEKVQKLSLNNVIPYVAPAQKLEFIRDKSIDFVFSNLMLCCTSDHDGALREIKRILKDSGLAYISVTRSFITKDKKDVSGKEWKEILSQFKVVKEGKSLLERWAIVQQKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XE2</accession>
    <name>Q97XE2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1212</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1804</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90342">
        <property type="entry name" value="H90342"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923600.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XE2"/>
    <dbReference type="STRING" id="273057.SSO1804"/>
    <dbReference type="PaxDb" id="273057-SSO1804"/>
    <dbReference type="EnsemblBacteria" id="AAK41999">
        <property type="protein sequence ID" value="AAK41999"/>
        <property type="gene ID" value="SSO1804"/>
    </dbReference>
    <dbReference type="GeneID" id="1454778"/>
    <dbReference type="KEGG" id="sso:SSO1804"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1861"/>
    <dbReference type="eggNOG" id="arCOG07925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154343_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XE2"/>
    <dbReference type="PhylomeDB" id="Q97XE2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="137" mass="15527" checksum="D3F74EC6929BD4A0" modified="2001-10-01" version="1">MGYTTSKLKSFTAFRKYKGWGRKHGKSYFGFKVCNLVERRTNFVRDFKVGLANLSDLAFSFDNVKLMADRAWIFREDVLVKGVGSAGLPVEGSGVEIREGKASSTTLRGVVMEVFFLNLYRDLEILSTRIRTKVLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XJ9</accession>
    <name>Q97XJ9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">dTDP-Glucose 4,6-dehydratase (RfbB-2)</fullName>
            <ecNumber evidence="2">4.2.1.46</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rfbB-2</name>
        <name evidence="2" type="ordered locus">SSO1734</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.46" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90334">
        <property type="entry name" value="F90334"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XJ9"/>
    <dbReference type="FunCoup" id="Q97XJ9">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1734"/>
    <dbReference type="PaxDb" id="273057-SSO1734"/>
    <dbReference type="EnsemblBacteria" id="AAK41933">
        <property type="protein sequence ID" value="AAK41933"/>
        <property type="gene ID" value="SSO1734"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1734"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1778"/>
    <dbReference type="eggNOG" id="arCOG01371">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_14_2"/>
    <dbReference type="InParanoid" id="Q97XJ9"/>
    <dbReference type="PhylomeDB" id="Q97XJ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="106"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="350" mass="41170" checksum="E0AE232998718175" modified="2001-10-01" version="1">MNRVQLSQRSSPIFPQPAQVAPTRLKQGTEEFLREKYFSEDRVNYLEADLAKSRLNFAGLVVFSCFICSSPPSYIQTFLQKFSSSQLFLYFIHLVFLREKRDSHTDVDRSIYKPQDFVSTNVFGTVNVLEAARRYNFKYVHISTDEVYGEECSDENSSVNPSSPYSASKASADLFVKSYIKTYNVEAIIVRPSNNYGPRQFPEKFIPKAIIRTILGIYVPIYGNGEQERDWIYVEDTARVIFDLIRSAEWRGEVYNIPGGYRVKNIQLIRLLERVIKKEIKVKYVSDRPGHDRRYCMINTKLNYTTTPLEEGLRKTYDWYANNNWWWSSLVNDEFFKSDEPWRPYEDKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XQ3</accession>
    <name>Q97XQ3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SpoVT-AbrB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO8620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90326">
        <property type="entry name" value="G90326"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989998.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XQ3"/>
    <dbReference type="STRING" id="273057.SSO8620"/>
    <dbReference type="PaxDb" id="273057-SSO8620"/>
    <dbReference type="EnsemblBacteria" id="AAK41870">
        <property type="protein sequence ID" value="AAK41870"/>
        <property type="gene ID" value="SSO8620"/>
    </dbReference>
    <dbReference type="GeneID" id="1454649"/>
    <dbReference type="KEGG" id="sso:SSO8620"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1706"/>
    <dbReference type="eggNOG" id="arCOG00816">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158484_9_3_2"/>
    <dbReference type="InParanoid" id="Q97XQ3"/>
    <dbReference type="PhylomeDB" id="Q97XQ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052975">
        <property type="entry name" value="Repressor-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860:SF6">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="79" mass="8859" checksum="751B9813839A77A5" modified="2001-10-01" version="1">MEEVKVTRNYQITIPYEIRQKLGIKIGDKLIVTVDGDKIVIEKKKGNISSLNLTLGKKITDEEINETINEAGREIADSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XQ9</accession>
    <name>Q97XQ9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1651</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99326">
        <property type="entry name" value="A99326"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992502.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XQ9"/>
    <dbReference type="PaxDb" id="273057-SSO1651"/>
    <dbReference type="EnsemblBacteria" id="AAK41864">
        <property type="protein sequence ID" value="AAK41864"/>
        <property type="gene ID" value="SSO1651"/>
    </dbReference>
    <dbReference type="GeneID" id="1454643"/>
    <dbReference type="KEGG" id="sso:SSO1651"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1699"/>
    <dbReference type="eggNOG" id="arCOG02221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135601_3_0_2"/>
    <dbReference type="InParanoid" id="Q97XQ9"/>
    <dbReference type="PhylomeDB" id="Q97XQ9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18689">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="3"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="131" mass="14673" checksum="8A34674A09B74193" modified="2001-10-01" version="1">MKVVTDTGVLVEVLEGSKLGEKFIQLVDSGKIEPIITNLTLIELSYIICRKYGIDKARELVKKLLDSNYFEVVNAFDFAENIVEIKCNNSLSIIDASVIATAKALGISALFKMEKELKDKKFNNLIFIENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XR6</accession>
    <name>Q97XR6_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1643</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90325">
        <property type="entry name" value="B90325"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923543.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XR6"/>
    <dbReference type="STRING" id="273057.SSO1643"/>
    <dbReference type="PaxDb" id="273057-SSO1643"/>
    <dbReference type="EnsemblBacteria" id="AAK41857">
        <property type="protein sequence ID" value="AAK41857"/>
        <property type="gene ID" value="SSO1643"/>
    </dbReference>
    <dbReference type="GeneID" id="1454636"/>
    <dbReference type="KEGG" id="sso:SSO1643"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1692"/>
    <dbReference type="eggNOG" id="arCOG01930">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043951_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XR6"/>
    <dbReference type="PhylomeDB" id="Q97XR6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42889">
        <property type="entry name" value="BLR3681 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42889:SF1">
        <property type="entry name" value="BLR3681 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="11"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="320" mass="37488" checksum="CD32994973D8D4A5" modified="2001-10-01" version="1">MAKGWIDCFYDYHKSLTPEDYFMDYNTFKYYGSNRMIEDVFINGYVDVAITQPQYLQYFYRESFGNTEEFGKLALNNPNRFVIGTRWDPRDGEEGKEKLEEDVRRYRVKPWQMRHVKLYTAEWKDVNGKLSRGWRLDSKEAFEFIEFSKSLGIDILVAHKGPTVWPLDKDAFDITDVDAAASSFPEIKFVVTHIGLPRLDDFVWTAVQDKNIYAGLAVASAFIHKRPRYFAQIMAELLFWLGPDRILMGSDYAIWNPKWILEEFAKFELPEDVKKEYGVELTLDIKKKILFENASKLWGIPLPPRDDEVGKRAKPLIVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XS7</accession>
    <name>Q97XS7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Coenzyme PQQ synthesis protein E (PqqE-3)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pqqE-3</name>
        <name evidence="8" type="ordered locus">SSO1632</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90323">
        <property type="entry name" value="F90323"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923532.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XS7"/>
    <dbReference type="FunCoup" id="Q97XS7">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1632"/>
    <dbReference type="PaxDb" id="273057-SSO1632"/>
    <dbReference type="EnsemblBacteria" id="AAK41845">
        <property type="protein sequence ID" value="AAK41845"/>
        <property type="gene ID" value="SSO1632"/>
    </dbReference>
    <dbReference type="GeneID" id="1454624"/>
    <dbReference type="KEGG" id="sso:SSO1632"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1674"/>
    <dbReference type="eggNOG" id="arCOG00938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_0_2"/>
    <dbReference type="InParanoid" id="Q97XS7"/>
    <dbReference type="PhylomeDB" id="Q97XS7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21123">
        <property type="entry name" value="SPASM_MftC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000325">
        <property type="entry name" value="Radical SAM domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034391">
        <property type="entry name" value="AdoMet-like_SPASM_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017200">
        <property type="entry name" value="PqqE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF7">
        <property type="entry name" value="PQQA PEPTIDE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037420">
        <property type="entry name" value="PQQ_syn_pqqE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01387">
        <property type="entry name" value="BtrN-like_SPASM_domain_contain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="32"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="378" mass="43083" checksum="C77335CC47F12EDE" modified="2001-10-01" version="1">MIPISVLVTNAGTVSFHIKGEYNKDRPSKFSDVFRPVVTWNLTRKCNLKCLHCYINASPEGEDGSTTEEALRLIDEMAEMRIPLIIMSGGEPLMRRDFFELASYARIKGIKLALSTNGTLITESVAKKLKELDFSYVGISLDSYDPEFHDRFRGLNGAFNMTVKGIKNAINVGLNVGLRFTITARNIHQIDEYVKLALELGVKRITFYHLSASGRGKELREWMYTPEEYVGFINKMIDYAIKLSGKIEIETTLGQFDGVYIAKKLARDEEELEKYMKFVENSGGCGRKMISIYPNGDVYPCQFIDFYKLGNVREKPLKEIIKNIPDLFINTDKYLRGEYCDKCKYKSACKGGDRARAYYWNGDVYGDDPLCPLKTLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XU3</accession>
    <name>Q97XU3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase FkbM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1613</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90321">
        <property type="entry name" value="E90321"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923516.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XU3"/>
    <dbReference type="SMR" id="Q97XU3"/>
    <dbReference type="STRING" id="273057.SSO1613"/>
    <dbReference type="PaxDb" id="273057-SSO1613"/>
    <dbReference type="EnsemblBacteria" id="AAK41828">
        <property type="protein sequence ID" value="AAK41828"/>
        <property type="gene ID" value="SSO1613"/>
    </dbReference>
    <dbReference type="GeneID" id="1454607"/>
    <dbReference type="KEGG" id="sso:SSO1613"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1655"/>
    <dbReference type="eggNOG" id="arCOG01400">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080078_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XU3"/>
    <dbReference type="PhylomeDB" id="Q97XU3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052514">
        <property type="entry name" value="SAM-dependent_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203">
        <property type="entry name" value="METHYLTRANSFERASE, FKBM FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203:SF15">
        <property type="entry name" value="SLL1173 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase FkbM" evidence="1">
        <location>
            <begin position="50"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="222" mass="25724" checksum="587B8E6C0F8F8C78" modified="2001-10-01" version="1">MFNIKDNYMEFTYKDKRLRFYGFLLNGHVYNQFISFEYGKIDFKNKTVIDIGANIGDSSIYFAINGAKEVYAIEPMPKLFNYLTENIRFNNITNIIPLNVAIGNEEGLIKIPNIDVGLDAYTKNFQNSENGNIIPVKTLRSIISEYVISENIVLKIDCEGCEYDAIYSLDNTTLDKISQIALEYHYGPYKLSEFLIEKGFKVEYTKPKRYNEHEIGILYAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y67</accession>
    <name>Q97Y67_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase ISC1476</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1475</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90305">
        <property type="entry name" value="G90305"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y67"/>
    <dbReference type="SMR" id="Q97Y67"/>
    <dbReference type="STRING" id="273057.SSO1475"/>
    <dbReference type="PaxDb" id="273057-SSO1475"/>
    <dbReference type="EnsemblBacteria" id="AAK41702">
        <property type="protein sequence ID" value="AAK41702"/>
        <property type="gene ID" value="SSO1475"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1475"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1514"/>
    <dbReference type="eggNOG" id="arCOG00683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040997_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y67"/>
    <dbReference type="PhylomeDB" id="Q97Y67"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="entry name" value="YqgF/RNase H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF21">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="167"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="297"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="416" mass="48649" checksum="46D482660744E178" modified="2001-10-01" version="1">MKRSNTVRLVVSKEQHEVLKRLGIATAKCWNEVNWLRMKQYKEGKRVDFGITEKAVYEKYKSVLRVNAQQVARKNAEDWRSFFSLTREKKEGKLPSWFKPRPPGYWKDGRLIVIIRNDRYTVDERNRVVYLKDFNLSLKFKGRLKWHGKQGRLEIIYDEARKIWYAHIPVEVETEKVKEGIKASIDLGIVNLATVYVEDGSWYLFKGGSALSHYENYSKRITIVQKTLHRHRQRRSRRLKLLHEKRSRFLKHALNSMVRRIMEILREKNVGEVVVGYPKEIAKHHGNKLTVNFWNYSYIIKRLEDIGEELGIKVITVDEAYTSKTCSLCGEAHEDGRVKRGLFVCPRTRKAINADLNGAINILHIPESRGSEGNFHRGIGVIGLKTQPVTLSDLSKIKNHYNYSISDYTILHYIQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y99</accession>
    <name>Q97Y99_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase S9 prolyl oligopeptidase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90301">
        <property type="entry name" value="D90301"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923399.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y99"/>
    <dbReference type="STRING" id="273057.SSO1433"/>
    <dbReference type="ESTHER" id="sulso-SSO1433">
        <property type="family name" value="AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1433"/>
    <dbReference type="EnsemblBacteria" id="AAK41667">
        <property type="protein sequence ID" value="AAK41667"/>
        <property type="gene ID" value="SSO1433"/>
    </dbReference>
    <dbReference type="GeneID" id="1454446"/>
    <dbReference type="KEGG" id="sso:SSO1433"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1460"/>
    <dbReference type="eggNOG" id="arCOG05706">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1375587_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y99"/>
    <dbReference type="PhylomeDB" id="Q97Y99"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="1">
        <location>
            <begin position="53"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="194" mass="22146" checksum="07F2F57C0E77BD40" modified="2001-10-01" version="1">MMKSLVIHGKNSSPEKVEWLAKPLRKFGEVIVPEFEIEAKEGVEKALTYDFDCIAGHSRGGLIALIAAALKGKWAIAVSAPSDRRKQKEYLSKFPPGTVQYNIYQDLMKMPEYELEYSSLSYADRLKKVLLIHGEDDEMVLKEHSITLCDTIKRMGGDCELHIIPKMKHTPLGQQYQTIWSIIEDWIKRNSSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YC3</accession>
    <name>Q97YC3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HD Cas3-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1403</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90297">
        <property type="entry name" value="G90297"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988400.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YC3"/>
    <dbReference type="STRING" id="273057.SSO1403"/>
    <dbReference type="PaxDb" id="273057-SSO1403"/>
    <dbReference type="DNASU" id="1454417"/>
    <dbReference type="EnsemblBacteria" id="AAK41638">
        <property type="protein sequence ID" value="AAK41638"/>
        <property type="gene ID" value="SSO1403"/>
    </dbReference>
    <dbReference type="GeneID" id="1454417"/>
    <dbReference type="KEGG" id="sso:SSO1403"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1419"/>
    <dbReference type="eggNOG" id="arCOG01443">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1286398_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YC3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10013">
        <property type="entry name" value="Cas3''_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006483">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038257">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01596">
        <property type="entry name" value="cas3_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51643">
        <property type="entry name" value="HD_CAS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD Cas3-type" evidence="4">
        <location>
            <begin position="5"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="214" mass="25060" checksum="46D02BED0D6C1E01" modified="2001-10-01" version="1">MTCWAFYGMETFKQHSLGILNFFRDNFSYIIPIISYRTGIDKETVRKSVEIGVSLHDIGKTSKYYDMSYFGHEFYSGYLVYKILRECCDSELKPLIALAAMSHHQGMEGRTLNEMILKGNYTRIPSFYELREECRNDIVEILGEIGVKVKDFPQKVTRSDVKSWFQKLNIKWKNLYVIILGPLMISDTVVANKNRGGDQYNKIIEEYEKWINVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YE4</accession>
    <name>Q97YE4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase ISC1217</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1382</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90295">
        <property type="entry name" value="A90295"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923366.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YE4"/>
    <dbReference type="PaxDb" id="273057-SSO1382"/>
    <dbReference type="EnsemblBacteria" id="AAK41616">
        <property type="protein sequence ID" value="AAK41616"/>
        <property type="gene ID" value="SSO1382"/>
    </dbReference>
    <dbReference type="GeneID" id="1454395"/>
    <dbReference type="KEGG" id="sso:SSO1382"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1387"/>
    <dbReference type="eggNOG" id="arCOG09888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077283_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YE4"/>
    <dbReference type="PhylomeDB" id="Q97YE4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006783">
        <property type="entry name" value="Transposase_ISC1217"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04693">
        <property type="entry name" value="DDE_Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="354" mass="40310" checksum="81CB749EBAB3A00C" modified="2001-10-01" version="1">MTEELTREEYYKALEKAVNEVILSMTGTRKDVAKRLLLGAVVGRNATEIAQEAEMDYETVLNNLDKAAQAKLIEIVKKLVGDHPVLLIIDDTHDHKLYARAMPVSRNGAQIFYCRAHKRFESAIQLLVIGVKDLVNNQIYVIHIIAYIPRKVEEELKRRGEEVKFKTKIDALLEFLSSLSGLNVKSKVFDSWYVNSRTLQGNTMGELKSSARVVEGGRSVPVGEFPQGEYLVEYLGTPIKLLVIDDYKGYGRRYFFSTDLNDTAEDIITTWENRWDIEVLIRELKALGLEGGSFLTWVRNSGFVALKALSLLVVQYFKYSTGLRLGAKRLARLIKSIYREAGGIKKLFKRRRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YE5</accession>
    <name>Q97YE5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1381</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90294">
        <property type="entry name" value="H90294"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992588.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YE5"/>
    <dbReference type="STRING" id="273057.SSO1381"/>
    <dbReference type="PaxDb" id="273057-SSO1381"/>
    <dbReference type="EnsemblBacteria" id="AAK41615">
        <property type="protein sequence ID" value="AAK41615"/>
        <property type="gene ID" value="SSO1381"/>
    </dbReference>
    <dbReference type="GeneID" id="1454394"/>
    <dbReference type="KEGG" id="sso:SSO1381"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1386"/>
    <dbReference type="eggNOG" id="arCOG01439">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1029036_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YE5"/>
    <dbReference type="PhylomeDB" id="Q97YE5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041165">
        <property type="entry name" value="Cas6_N_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019267">
        <property type="entry name" value="CRISPR-assoc_Cas6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010156">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01877">
        <property type="entry name" value="cas_cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17952">
        <property type="entry name" value="Cas6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10040">
        <property type="entry name" value="CRISPR_Cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas6 N-terminal" evidence="6">
        <location>
            <begin position="2"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="CRISPR-associated protein Cas6 C-terminal" evidence="5">
        <location>
            <begin position="103"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="233" mass="27317" checksum="6A9FB450BEAF7CB8" modified="2001-10-01" version="1">MFLSFSPTYSKITESKEPHKPLRITVIKDHGKPLYSTGKNKIVLKTENTYTFTVNTLLEDVVKEVVKTESVNKEIYNTKFHVELENIIVKEEFKINDARFYKIHFKTPTLLQPPRPSIKRKGNRYVLFPYVPLLFYSIASHWNRYMNNKIVGVTGSKTLYYFREVNYKIRPLTAYYGNIPNKGFVGWVLFELKARKGSNLRENIRRLLDYANYFGIGKSRNIGFGEVDVRPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YF3</accession>
    <name>Q97YF3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Partial transposase ISC1173</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1372</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Absolutely required for transposition of IS1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase 27 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90293">
        <property type="entry name" value="G90293"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YF3"/>
    <dbReference type="PaxDb" id="273057-SSO1372"/>
    <dbReference type="EnsemblBacteria" id="AAK41606">
        <property type="protein sequence ID" value="AAK41606"/>
        <property type="gene ID" value="SSO1372"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1372"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1377"/>
    <dbReference type="eggNOG" id="arCOG02133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135438_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YF3"/>
    <dbReference type="PhylomeDB" id="Q97YF3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005063">
        <property type="entry name" value="Transposase_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051354">
        <property type="entry name" value="Transposase_27_IS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33293:SF1">
        <property type="entry name" value="INSERTION ELEMENT IS1 1 PROTEIN INSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03400">
        <property type="entry name" value="DDE_Tnp_IS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="129" mass="15376" checksum="708F772155144F5D" modified="2001-10-01" version="1">MYGVMREDLWIWTVLADGVPFYEFGDRSYGTFRYLLGWLTRSEVNYTDYYCVYQVLDNRVAGKKYTYLVESHNSWCRLARLARDTKAVNRSIRMAEYSLALSNVMYPRVFSREGTPLNEAYLKEVQYIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YI1</accession>
    <name>Q97YI1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetyl-CoA synthetase (Acetate-CoA ligase) amino-end. (AcsA-2)</fullName>
            <ecNumber evidence="6">6.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">acsA-2</name>
        <name evidence="6" type="ordered locus">SSO1342</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90290">
        <property type="entry name" value="C90290"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YI1"/>
    <dbReference type="SMR" id="Q97YI1"/>
    <dbReference type="FunCoup" id="Q97YI1">
        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1342"/>
    <dbReference type="PaxDb" id="273057-SSO1342"/>
    <dbReference type="EnsemblBacteria" id="AAK41578">
        <property type="protein sequence ID" value="AAK41578"/>
        <property type="gene ID" value="SSO1342"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1342"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1346"/>
    <dbReference type="eggNOG" id="arCOG04201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_968455_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YI1"/>
    <dbReference type="PhylomeDB" id="Q97YI1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051087">
        <property type="entry name" value="Mitochondrial_ACSM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605:SF10">
        <property type="entry name" value="ACYL-COA SYNTHETASE MEDIUM CHAIN FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605">
        <property type="entry name" value="ACYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="44"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="287" mass="32614" checksum="C756759EF9ABAA27" modified="2001-10-01" version="1">MLEVDLREIRFHNYDELVRTFKWNIPSRFNIGESILDRKVKEGVGDNIAIYYEDEEGDHFVYTFAQLKSLSDSLITILREIGVKRGDVVGIYLQPRVETVISILSLYRLGAISLSISPLMGVEAVEYRIRQSDAKAIIIEGSRKEVREKLRNVTKIIVADEARDESEINFDEVKKTSGVHYAVDTKSDDPAQLFYTSGSTGAPKGVLHAHRFLLGHIPAYQLYFEMAPREEDVFYTPADWGWIGAIGDVILPSLYFGKPIVAYRRLGKFSPKDTLAIMQKCELPPHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YJ4</accession>
    <name>Q97YJ4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1329</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90288">
        <property type="entry name" value="F90288"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923322.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YJ4"/>
    <dbReference type="PaxDb" id="273057-SSO1329"/>
    <dbReference type="EnsemblBacteria" id="AAK41565">
        <property type="protein sequence ID" value="AAK41565"/>
        <property type="gene ID" value="SSO1329"/>
    </dbReference>
    <dbReference type="GeneID" id="1454344"/>
    <dbReference type="KEGG" id="sso:SSO1329"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1329"/>
    <dbReference type="eggNOG" id="arCOG05713">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_671964_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YJ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="401" mass="47543" checksum="6F41D7448B9E56DE" modified="2001-10-01" version="1">MNRRDRALLKKVGSYYLQYKGILDVEKLLSTFNGTEEVPLDDYTLSYIYYYLMYLITASVVRSAEDAKKSRLTYREFSIEYSNDLYGELNVDLSIPVYSMGLVAYHSFTEGYNAPEYGILGYITRRIYSIIKEKRSKIEVRKEQVPRYFNFLDDFDQTFSQLDKIKEEFPDGYFRDPSYTDPDWLLRAYKAYYTAKELEEIKVGIRTPKRPINKKLIKFIMWKLYELYVFYLVVNYLESKGYEIKKEEEKYIAIRGNRRLSIIFNASLSQSSLRKVDYIEDIEKYKGRPDISLVNERPIIFECKYSSKVSYITMGRFKIMAYTYEYDPSLAVLVYPGLEIKQINFDNEDEATMELDGIARAKSGILDFWYNSHLLYMIIIDPLESDENNLSKIDKIMKEHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="124" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YK2</accession>
    <name>Q97YK2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Antibiotic ABC transporter, ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1319</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90287">
        <property type="entry name" value="F90287"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923314.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YK2"/>
    <dbReference type="FunCoup" id="Q97YK2">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1319"/>
    <dbReference type="PaxDb" id="273057-SSO1319"/>
    <dbReference type="EnsemblBacteria" id="AAK41557">
        <property type="protein sequence ID" value="AAK41557"/>
        <property type="gene ID" value="SSO1319"/>
    </dbReference>
    <dbReference type="GeneID" id="1454336"/>
    <dbReference type="KEGG" id="sso:SSO1319"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1321"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q97YK2"/>
    <dbReference type="PhylomeDB" id="Q97YK2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF18">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="297" mass="33537" checksum="A00D03E555834DE4" modified="2001-10-01" version="1">MIVVSNIVKKYGNKIALDNVSLEVKKREFVSLIGPNGAGKTTLIRILLTLMKPDRGEVQILGENPFKSKRIFKEVGYVQEIPNYPPFLTGKQVLELSAKIRGVDKSEVNRVLEFVEMENYANNPIVKYSKGMVQRIAIAEALLGNPSILIMDEPNMGIDPIFSLKTRELLNKLKRKDNVSILMTSHELEDVKKLSDRIFMIYKGKIVFSGTVEDMVKEFLGIQVVVETDEIEKAEGVLKGIEYVKGIFNDGNRLIAKLNEDRREELLKDLVTSGVKVKGFYIDLNLEEAYMRALGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YL1</accession>
    <name>Q97YL1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ring oxydation complex/ phenylacetic acid degradation related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1312</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90286">
        <property type="entry name" value="E90286"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YL1"/>
    <dbReference type="STRING" id="273057.SSO1312"/>
    <dbReference type="PaxDb" id="273057-SSO1312"/>
    <dbReference type="EnsemblBacteria" id="AAK41548">
        <property type="protein sequence ID" value="AAK41548"/>
        <property type="gene ID" value="SSO1312"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1312"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1313"/>
    <dbReference type="eggNOG" id="arCOG04786">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093768_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YL1"/>
    <dbReference type="PhylomeDB" id="Q97YL1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052703">
        <property type="entry name" value="Aromatic_CoA_ox/epox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007814">
        <property type="entry name" value="PaaA_PaaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30458:SF0">
        <property type="entry name" value="1,2-PHENYLACETYL-COA EPOXIDASE, SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30458">
        <property type="entry name" value="PHENYLACETIC ACID DEGRADATION PROTEIN PAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05138">
        <property type="entry name" value="PaaA_PaaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="261" mass="30721" checksum="5508C9DC4ACE9B86" modified="2001-10-01" version="1">MCKYISMIPVEYKKPKRVIGWGDYKGLRKFESIYDLPFEAVDVLLKLLTVQGDTEFASIEQHMPWLFHAPKLSDRVTISRIMVDEMRHGWQVIQLLKEFGEEGKKIAENLLWTRMGRHKLDAFNIPFNMWEDTLGFTFLIDRVGLYQLLAFEDSSFGPLSRIIPTMLMEEEFHINFGYTGIKRLVEEGKRDLAQALINKWFPRGLDMFGHSKSYTIDLAYKYGIKRMRNDEMRQLYIKDVAELIAPLGLELPDVNRNRRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YL5</accession>
    <name>Q97YL5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Circularly permuted ATP-grasp type 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1308</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99286">
        <property type="entry name" value="A99286"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YL5"/>
    <dbReference type="STRING" id="273057.SSO1308"/>
    <dbReference type="PaxDb" id="273057-SSO1308"/>
    <dbReference type="DNASU" id="1454323"/>
    <dbReference type="EnsemblBacteria" id="AAK41544">
        <property type="protein sequence ID" value="AAK41544"/>
        <property type="gene ID" value="SSO1308"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1308"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1309"/>
    <dbReference type="eggNOG" id="arCOG07294">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017048_2_0_2"/>
    <dbReference type="InParanoid" id="Q97YL5"/>
    <dbReference type="PhylomeDB" id="Q97YL5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051680">
        <property type="entry name" value="ATP-dep_Glu-Cys_Ligase-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025841">
        <property type="entry name" value="CP_ATPgrasp_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016450">
        <property type="entry name" value="UCP005522"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34595">
        <property type="entry name" value="BLR5612 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34595:SF7">
        <property type="entry name" value="SLL1039 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14403">
        <property type="entry name" value="CP_ATPgrasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005522">
        <property type="entry name" value="UCP005522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Circularly permuted ATP-grasp type 2" evidence="1">
        <location>
            <begin position="75"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="449" mass="52093" checksum="7F3CE7A8FD01333B" modified="2001-10-01" version="1">MYYISLNVKRRKLNKDNNYNEFLTDPIYSRLIENVEKLKDKVFHLSKIVNLQAYMEGFTFYTSSNYRSTPIDVIPRIITSELFFKISNYLVARSYVLNKFIREVYQEGSNPIPDWIVKTTPYFRPEMMGFSPPKDIYIHVYGADIVRVNGVPFILEDNLRIPSGISYSYKAFEYVDRFLPELKEGYDNRVLQINGLDYLYDILRYVSGNKDPIIVILTDGNYNSAYFEHKFISEKLGIILAEPNDIYVKDKEVVVRTVDEGEVHVDVIYRRIEDLDYLTPGLMKAYLRGWVTLANAPGTGVADDKATFVWIPYLAEKYGIKLENVIQPYTICLYEKENLEKVITQPTRYVIKKREGYGGIGLAIVKDDNINVLKEIYKEYENFIAQEVLDFDTVISVVNDSFYETYADMRIFTYYDKVASAVLSRVGIMGSRITNNSSGGMVKPLWIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="108" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YS6</accession>
    <name>Q97YS6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Toluene-4-monooxygenase system protein E. (TmoE)</fullName>
            <ecNumber evidence="3">1.14.13.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tmoE</name>
        <name evidence="3" type="ordered locus">SSO1233</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.13.-" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90277">
        <property type="entry name" value="G90277"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992604.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YS6"/>
    <dbReference type="FunCoup" id="Q97YS6">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1233"/>
    <dbReference type="PaxDb" id="273057-SSO1233"/>
    <dbReference type="EnsemblBacteria" id="AAK41478">
        <property type="protein sequence ID" value="AAK41478"/>
        <property type="gene ID" value="SSO1233"/>
    </dbReference>
    <dbReference type="GeneID" id="1454257"/>
    <dbReference type="KEGG" id="sso:SSO1233"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1232"/>
    <dbReference type="eggNOG" id="arCOG08361">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061948_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YS6"/>
    <dbReference type="PhylomeDB" id="Q97YS6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01058">
        <property type="entry name" value="AAMH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012078">
        <property type="entry name" value="MP_mOase_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003430">
        <property type="entry name" value="Phenol_Hydrox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02332">
        <property type="entry name" value="Phenol_Hydrox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000040">
        <property type="entry name" value="MMOH_comp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="376" mass="45080" checksum="7D6140073761AB4E" modified="2001-10-01" version="1">MTRLEDLEWYKRYKQMFGAFKSGPEGDPFFRDYEYRGYKKVWSTWPMLEKKLGRKKPSEYQVVTYALSYWADPRSPTYIYDKGPFELGTEHITQKWYKHFRDNSPFIKPLFERGEWHDYEDPYKLTYWTYNSMADDNETFLDKIYEEIVNTKYDWNLNEEVLELYKNVYDPLRYVFHIMQMESMYLATMAPTSSIANVFIFMGMDHLRRVQRISQRVKMLDIVYPSLGFGKETRKVFEESPIFQPTREVLEKMLVTYDVGEALVAFNLAVKFVLDELILQHLTQPFSKLGDEMIKHIHLSFYNDTLRHRHQAQELFKYAFSKEPSLKDVIKPWVKDWQEMGFKATEGFRDVLKGEYDNAIRQIRKAHSEYLGGIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YU4</accession>
    <name>Q97YU4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Hydrogenase formation/expression factor related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1213</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A31 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90275">
        <property type="entry name" value="E90275"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923251.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YU4"/>
    <dbReference type="STRING" id="273057.SSO1213"/>
    <dbReference type="PaxDb" id="273057-SSO1213"/>
    <dbReference type="EnsemblBacteria" id="AAK41460">
        <property type="protein sequence ID" value="AAK41460"/>
        <property type="gene ID" value="SSO1213"/>
    </dbReference>
    <dbReference type="GeneID" id="1454239"/>
    <dbReference type="KEGG" id="sso:SSO1213"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1211"/>
    <dbReference type="eggNOG" id="arCOG04429">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631731_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YU4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06070">
        <property type="entry name" value="H2MP_like-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="entry name" value="HybD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="HYDROGENASE 1 MATURATION PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302:SF1">
        <property type="entry name" value="HYDROGENASE 2 MATURATION PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01750">
        <property type="entry name" value="HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="HybD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="162" mass="18168" checksum="67586E1D0B0504E1" modified="2001-10-01" version="1">MKAIIGVGNRLMKDDGFGSCLAEVIINKVKNAVVVDLGLGNLLSVDLDKYDTVIIIDVANINEEYGIFKITSTSKGILEQSLHELGLNTILKLYEDKQFYIIVCKPEEIQIGYGLSKDCLLRIEKLIPEFKAFLKKLGIDADFNVMDIIEEIKERCVNTLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YV1</accession>
    <name>Q97YV1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MCM C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90274">
        <property type="entry name" value="E90274"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923246.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YV1"/>
    <dbReference type="STRING" id="273057.SSO1204"/>
    <dbReference type="PaxDb" id="273057-SSO1204"/>
    <dbReference type="EnsemblBacteria" id="AAK41452">
        <property type="protein sequence ID" value="AAK41452"/>
        <property type="gene ID" value="SSO1204"/>
    </dbReference>
    <dbReference type="GeneID" id="1454231"/>
    <dbReference type="KEGG" id="sso:SSO1204"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1203"/>
    <dbReference type="eggNOG" id="arCOG03169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2115588_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YV1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048907">
        <property type="entry name" value="WHD_MCM_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21100">
        <property type="entry name" value="WHD_MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal" evidence="1">
        <location>
            <begin position="43"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="114" mass="13393" checksum="20456AEA5CF39614" modified="2001-10-01" version="1">MTLFGNFYAVRKLDFEIALRETIGEGKKIMLEEFNHFLSNKENKQLYCNIMNVLKLASKWKDIKNGVEIRMGKVDDKVFSNALQNLVNFNFVSKVDDEYKIVDLMLKEIDFNKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YV9</accession>
    <name>Q97YV9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Exopolyphosphatase (Metaphosphatase), putative</fullName>
            <ecNumber evidence="2">3.6.1.11</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1193</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.1.11" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90273">
        <property type="entry name" value="C90273"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992804.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YV9"/>
    <dbReference type="SMR" id="Q97YV9"/>
    <dbReference type="FunCoup" id="Q97YV9">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1193"/>
    <dbReference type="PaxDb" id="273057-SSO1193"/>
    <dbReference type="DNASU" id="1454221"/>
    <dbReference type="EnsemblBacteria" id="AAK41442">
        <property type="protein sequence ID" value="AAK41442"/>
        <property type="gene ID" value="SSO1193"/>
    </dbReference>
    <dbReference type="GeneID" id="1454221"/>
    <dbReference type="KEGG" id="sso:SSO1193"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1193"/>
    <dbReference type="eggNOG" id="arCOG05138">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025908_4_2_2"/>
    <dbReference type="InParanoid" id="Q97YV9"/>
    <dbReference type="PhylomeDB" id="Q97YV9"/>
    <dbReference type="BRENDA" id="3.6.1.11">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004309">
        <property type="term" value="F:exopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd24052">
        <property type="entry name" value="ASKHA_NBD_HpPPX-GppA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050273">
        <property type="entry name" value="GppA/Ppx_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003695">
        <property type="entry name" value="Ppx_GppA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30005">
        <property type="entry name" value="EXOPOLYPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30005:SF0">
        <property type="entry name" value="RETROGRADE REGULATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02541">
        <property type="entry name" value="Ppx-GppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ppx/GppA phosphatase N-terminal" evidence="1">
        <location>
            <begin position="22"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="417" mass="46106" checksum="488B009488E7A78A" modified="2001-10-01" version="1">MISAVIDCGYNSFRMVVYQVFRNGTFRALGSSKSFVRIGEGLKEGDTIPEEKVEKAERTFTIFKRILNGINVDEVKIVATSAFRYASNGNEVRLRLSKIIENEVRVISGEEEGSYAALGMLNTLPIPDGIFFELGGGSLEIAEVTSGNIIRVHQLPIGALKLVSLPEREIRKKVSDELSTINIKKANIMVGSGGNVRALAKLDLKLSSFPTKSVHGYLLSSKQISKYASLLPSLDDESRKSLPGISKERALTIHSASVIVDELIKYLNGSDMIVSLFGMREGVLTEGKKLDKMNWLEEISYSNAIDPPFGIFKEVMSEVDSKYSFYVASSALLSLIFKMVGYFNPFRACYRFIKESVLPGFTLDEVLLIGLICEAASGKVKKKHVKLLKDDITKKELLSFGNIVKNSIDKYVVGVRV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2014-05-14" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YX6</accession>
    <name>HJE_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Crossover junction endodeoxyribonuclease Hje</fullName>
            <shortName evidence="6">Hje</shortName>
            <ecNumber evidence="5">3.1.21.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Holliday junction resolvase Hje</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hje</name>
        <name type="ordered locus">SSO1176</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Mol. Biol." volume="295" first="193" last="202">
            <title>An archaeal Holliday junction resolving enzyme from Sulfolobus solfataricus exhibits unique properties.</title>
            <authorList>
                <person name="Kvaratskhelia M."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="10623519"/>
            <dbReference type="DOI" id="10.1006/jmbi.1999.3363"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2000" name="J. Mol. Biol." volume="297" first="923" last="932">
            <title>Two Holliday junction resolving enzymes in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Kvaratskhelia M."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="10736227"/>
            <dbReference type="DOI" id="10.1006/jmbi.2000.3624"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="350" first="1" last="6">
            <title>The endonuclease Hje catalyses rapid, multiple turnover resolution of Holliday junctions.</title>
            <authorList>
                <person name="Parker J.L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="15921693"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.04.056"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2003" name="Acta Crystallogr. D" volume="59" first="171" last="173">
            <title>Crystallization and preliminary X-ray diffraction studies of Hje, a HolliDay junction resolving enzyme from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Middleton C.L."/>
                <person name="Parker J.L."/>
                <person name="Richard D.J."/>
                <person name="White M.F."/>
                <person name="Bond C.S."/>
            </authorList>
            <dbReference type="PubMed" id="12499561"/>
        </citation>
        <scope>PRELIMINARY CRYSTALLIZATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="5442" last="5451">
            <title>Substrate recognition and catalysis by the Holliday junction resolving enzyme Hje.</title>
            <authorList>
                <person name="Middleton C.L."/>
                <person name="Parker J.L."/>
                <person name="Richard D.J."/>
                <person name="White M.F."/>
                <person name="Bond C.S."/>
            </authorList>
            <dbReference type="PubMed" id="15479781"/>
            <dbReference type="DOI" id="10.1093/nar/gkh869"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF SER-30</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3 5">A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Acts only on 4-way DNA junctions in a sequence non-specific manner; introduces paired nicks in opposing strands 2 bases 3' of the point of strand exchange only on continuous strands of 4-way junction DNA. Cleaves both mobile and immobile junctions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2 5">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2 5">73 nM for 4-way junction DNA at 5 mM MgCl2</KM>
            <KM evidence="2 5">66 nM for 4-way junction DNA at 15 mM MgCl2</KM>
            <text>kcat is 4.8 min(-1) and 10 min(-1) at 5 mM and 15 mM MgCl2 respectively.</text>
        </kinetics>
        <temperatureDependence>
            <text evidence="2 5">Optimum temperature is 60 degrees Celsius. Could be higher with a more thermally stable substrate.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">A second Holliday junction resolving enzyme, Hjc, with different substrate specificity exists in this organism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the Holliday junction resolvase Hjc family. Hje subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90271">
        <property type="entry name" value="A90271"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991563.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1OB8">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B=1-135"/>
    </dbReference>
    <dbReference type="PDB" id="1OB9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-135"/>
    </dbReference>
    <dbReference type="PDBsum" id="1OB8"/>
    <dbReference type="PDBsum" id="1OB9"/>
    <dbReference type="AlphaFoldDB" id="Q97YX6"/>
    <dbReference type="SMR" id="Q97YX6"/>
    <dbReference type="STRING" id="273057.SSO1176"/>
    <dbReference type="PaxDb" id="273057-SSO1176"/>
    <dbReference type="EnsemblBacteria" id="AAK41424">
        <property type="protein sequence ID" value="AAK41424"/>
        <property type="gene ID" value="SSO1176"/>
    </dbReference>
    <dbReference type="GeneID" id="1454203"/>
    <dbReference type="GeneID" id="44130107"/>
    <dbReference type="KEGG" id="sso:SSO1176"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1169"/>
    <dbReference type="eggNOG" id="arCOG00919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881165_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YX6"/>
    <dbReference type="PhylomeDB" id="Q97YX6"/>
    <dbReference type="BRENDA" id="3.1.21.10">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q97YX6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00523">
        <property type="entry name" value="Holliday_junction_resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002732">
        <property type="entry name" value="Hjc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014428">
        <property type="entry name" value="Hjc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040854">
        <property type="entry name" value="Hol_resolv_Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651">
        <property type="entry name" value="HOLLIDAY JUNCTION RESOLVASE HJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651:SF1">
        <property type="entry name" value="HOLLIDAY JUNCTION RESOLVASE HJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01870">
        <property type="entry name" value="Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004985">
        <property type="entry name" value="Hlld_jn_rslvs_ar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000429159" description="Crossover junction endodeoxyribonuclease Hje">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="100- to 1000-fold reduction in kcat." evidence="4">
        <original>S</original>
        <variation>A</variation>
        <variation>C</variation>
        <variation>T</variation>
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="23"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="32"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="39"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="91"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="95"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="102"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="107"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="111"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="114"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10623519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="10736227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="15479781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="15921693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="6">
        <source>
            <dbReference type="PubMed" id="10623519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="1OB8"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="1OB9"/>
        </source>
    </evidence>
    <sequence length="135" mass="15452" checksum="13036E9997223BC2" modified="2001-10-01" version="1">MNRDIGKNAERELVSILRGEGFNAVRIPTSNSSPNPLPDIFATKGNTLLSIECKSTWENKVKVKEHQVRKLLDFLSMFTMKGVPLIAIKFKQVHEWRVLVPEKAEDIIVTIDNSIPIEDLFKILEKRIEEKILTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YX8</accession>
    <name>Q97YX8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Na(+)/Ca(2+) exchanging protein related</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1174</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="G90270"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991565.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YX8"/>
    <dbReference type="FunCoup" id="Q97YX8">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1174"/>
    <dbReference type="PaxDb" id="273057-SSO1174"/>
    <dbReference type="EnsemblBacteria" id="AAK41422">
        <property type="protein sequence ID" value="AAK41422"/>
        <property type="gene ID" value="SSO1174"/>
    </dbReference>
    <dbReference type="GeneID" id="1454201"/>
    <dbReference type="KEGG" id="sso:SSO1174"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1167"/>
    <dbReference type="eggNOG" id="arCOG02881">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077877_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YX8"/>
    <dbReference type="PhylomeDB" id="Q97YX8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:calcium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="INNER MEMBRANE PROTEIN YRBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="60"/>
        </location>
    </feature>
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            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <begin position="129"/>
            <end position="145"/>
        </location>
    </feature>
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            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="5"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="166"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="307" mass="33728" checksum="DCC6926D8462D0E4" modified="2001-10-01" version="1">MLLFWLEFIGIFIAIFVSAELLAKGADELEDFLGQGITGGIILGFLTALPETIFVIVASLEGSFDVALGSAIGGNVLLFTLGIGLVGVFYAFKWRTALQISSEYSVENVFLVLTTIGMLLVLIYGKLNIISGLLLILIYVIYVVYRVAKFRREGMEREKGNLMKPVGFIIAGGVLLVVFSHYFVEYINEIAIMFNVPAIWLSLVIAPIAGELEEKISAFRLVLMSKDGGSLSILSFVGSKIENATVLLGIIGIFTDYELQQALPEYASSLIANMIALYVLFDKRLKTFESVILIGVYVIIVILSFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YY7</accession>
    <name>Q97YY7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycoside hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90269">
        <property type="entry name" value="F90269"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YY7"/>
    <dbReference type="STRING" id="273057.SSO1163"/>
    <dbReference type="CAZy" id="GH15">
        <property type="family name" value="Glycoside Hydrolase Family 15"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO1163"/>
    <dbReference type="EnsemblBacteria" id="AAK41413">
        <property type="protein sequence ID" value="AAK41413"/>
        <property type="gene ID" value="SSO1163"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1163"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1157"/>
    <dbReference type="eggNOG" id="arCOG03286">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1029040_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YY7"/>
    <dbReference type="PhylomeDB" id="Q97YY7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616:SF13">
        <property type="entry name" value="GLUCAN 1,4-ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="30755" checksum="D2C3DBE2C8563E3E" modified="2001-10-01" version="1">MKYQIKEITESNYHLLPQDLSVWEDRDAYHFWTEAFNVLGLQSVAEIYKALNFSNYTIVLNYEKELNQSILKNFWNNDYFASALGVSVIFEGGKSESILTPEPPSVDSATLLPIDFGYLPPTSNYSISNFKLVNRTLFLTGGLARFPNDMYHYSQSLYDASAPEPPWMITTFFEALYLEELGKSNEALNLMYWAYNHSQHGLLPEAIDPKYAYPLPTTSPLTWSSAMFVITALNYKPPSESQSVSPLLYVIIVIILATIVITVISVLRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="98" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z04</accession>
    <name>Q97Z04_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transmembrane type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO1143</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="AAK41394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="C90267"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="AlphaFoldDB" id="Q97Z04"/>
    <dbReference type="STRING" id="273057.SSO1143"/>
    <dbReference type="PaxDb" id="273057-SSO1143"/>
    <dbReference type="EnsemblBacteria" id="AAK41394">
        <property type="protein sequence ID" value="AAK41394"/>
        <property type="gene ID" value="SSO1143"/>
    </dbReference>
    <dbReference type="GeneID" id="15297458"/>
    <dbReference type="KEGG" id="sso:SSO1143"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1134"/>
    <dbReference type="eggNOG" id="arCOG01468">
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    <dbReference type="HOGENOM" id="CLU_1163753_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z04"/>
    <dbReference type="PhylomeDB" id="Q97Z04"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ABC2_TM"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR30413:SF8">
        <property type="entry name" value="TRANSPORT PERMEASE PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="ABC_TM2"/>
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            <end position="229"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAK41394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="237" mass="26607" checksum="4C62FB542AA55CFA" modified="2001-10-01" version="1">MLSEFLNLIVMQLKMIRAYLPVFLFFSILFPIGFMLVFGYISIRSLTPFIVAGTITFYVSIGVLTSVAQSLAFERNAGRFSLMIATGIPREFYAISIALSNGIATLIMIPIILVLGSYLLHVEIKSIPFLLIALVSSLFMASMLGMALGLGIRNIYAVNQYSTIISFVLSFFAPVYFPVAFIPLPFRYLTYIEPTTYVSQALYYAFIGNPLSLLWSLGIIIFGFIFVILSRYVIRRQ</sequence>
</entry>
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    <accession>Q97Z22</accession>
    <name>Q97Z22_SACS2</name>
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        <recommendedName>
            <fullName evidence="4">PIN domain-containing protein</fullName>
        </recommendedName>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2013" name="J. Biomol. NMR" volume="57" first="363" last="368">
            <title>A PilT N-terminus domain protein SSO1118 from hyperthemophilic archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Xuan J."/>
                <person name="Song X."/>
                <person name="Chen C."/>
                <person name="Wang J."/>
                <person name="Feng Y."/>
            </authorList>
            <dbReference type="PubMed" id="24170369"/>
            <dbReference type="DOI" id="10.1007/s10858-013-9794-y"/>
        </citation>
        <scope>STRUCTURE BY NMR OF 40-150</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="H90264"/>
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        <property type="chains" value="A=40-150"/>
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    <dbReference type="EnsemblBacteria" id="AAK41375">
        <property type="protein sequence ID" value="AAK41375"/>
        <property type="gene ID" value="SSO1118"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1118"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1115"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PDB" id="2MDT"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="16270" checksum="22F5F38B5DA69D02" modified="2001-10-01" version="1">MDNLLKFSGFNISSSGSTVDTLILLYPKLINLACLYIFNMFAVISPSAFGKLKEILGSNKNYKFVITTLGVSFAIKSGIDIDSALDRGVIVRAFSHKPPKVGNLPQYESEAIMVAFELNALLIAEDKDVINKAKELGVNAIPIEELLASS</sequence>
</entry>
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    <accession>Q97Z47</accession>
    <name>Q97Z47_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1088</name>
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    <organism evidence="7 8">
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        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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            </authorList>
            <dbReference type="PubMed" id="11427726"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="PaxDb" id="273057-SSO1088"/>
    <dbReference type="EnsemblBacteria" id="AAK41349">
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        <property type="gene ID" value="SSO1088"/>
    </dbReference>
    <dbReference type="GeneID" id="1454128"/>
    <dbReference type="KEGG" id="sso:SSO1088"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1087"/>
    <dbReference type="eggNOG" id="arCOG05332">
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    <dbReference type="HOGENOM" id="CLU_1088271_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22680">
        <property type="entry name" value="FHA_ArnA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="Zn_Ribbon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="5">
        <location>
            <begin position="131"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="26"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="23232" checksum="A1F713B85CF20EDB" modified="2001-10-01" version="1">MTWRCPVCGYENADDSLFCIKCGTKKPEQQVEQPPAPPVEQQASGQQSVEQQPADQNQLSQQSATSEQQPVTEQQAPQQVVVEQPSETAKTEQPQSATTTVSKYYLLFINTPNPAFNKTKLPLDFDIFPSISIGRSPENVIVIPDPEVSRKHAIISFENNELYLEDLNSTNGTYVYDGKVFQPVKGKIKVQHNSIIKLGNNTVVRIVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z60</accession>
    <name>Q97Z60_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">First ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1073</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90259">
        <property type="entry name" value="H90259"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z60"/>
    <dbReference type="STRING" id="273057.SSO1073"/>
    <dbReference type="PaxDb" id="273057-SSO1073"/>
    <dbReference type="EnsemblBacteria" id="AAK41335">
        <property type="protein sequence ID" value="AAK41335"/>
        <property type="gene ID" value="SSO1073"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1073"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1070"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="InParanoid" id="Q97Z60"/>
    <dbReference type="PhylomeDB" id="Q97Z60"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2170:FF:000002">
        <property type="entry name" value="First ORF in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1390:FF:000002">
        <property type="entry name" value="ORF1 in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="4">
        <location>
            <begin position="8"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="146" mass="16974" checksum="4B444B89A2640667" modified="2001-10-01" version="1">MGIIRKRKVILYARVSSNTQKDDLVNQVKYLEEQVKEYDQVITDIGSKLNMKRKGFLKLLRMILNNEVSRVVVAYPDKLVRFGFEILEETYKAHGCEIVVINQEDKTPEELVEDLVSTLVSFSGKLYGMRSHKYEKVKKCAEELKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z61</accession>
    <name>Q97Z61_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Second ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1072</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90259">
        <property type="entry name" value="G90259"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923187.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z61"/>
    <dbReference type="STRING" id="273057.SSO1072"/>
    <dbReference type="PaxDb" id="273057-SSO1072"/>
    <dbReference type="EnsemblBacteria" id="AAK41334">
        <property type="protein sequence ID" value="AAK41334"/>
        <property type="gene ID" value="SSO1072"/>
    </dbReference>
    <dbReference type="GeneID" id="1454113"/>
    <dbReference type="KEGG" id="sso:SSO1072"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1069"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="InParanoid" id="Q97Z61"/>
    <dbReference type="PhylomeDB" id="Q97Z61"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF22">
        <property type="entry name" value="TNPB-LIKE PROTEIN MJ1635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="188"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="309"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="422" mass="50155" checksum="132C9ED380ECE181" modified="2001-10-01" version="1">MLKNLRIRKFEPEEEYVHFTYSIKNSEREKSKELIKEYRTLLQKAIDYLWNLTKIQVRKKNGNYKITLPKKKEVYKPLREELEKINHLASHYVDKAINDAFSILKSWRKRAIKGRASIEKPRVKKAYVRIKTTLRKVVGESVRITVRPHEYITFPWSKSWFSRRVRELELGEPIIKEEKVYLPFRYKLPWVTPVNFLAIDSNLYTLDAYDGEKFVTISLKQLYSLKYSMEVKRAKVQSFASKHTKRGRELLRKYSHRERNRVLDFVHKFVNTLLDLYPMTFFAVEKLNKESMFKDANGSLSRKISRTVWRSIHRVLKYKAPLYGSFVKEVNPHLTSRSCPRCGFVSRKVGKTFECERCGFKLDRQLNASLNIYLKMCGFPHIREIARVWVGVIPLMGRRGMNVRDFGEAQGLRVDIKYHEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZB2</accession>
    <name>Q97ZB2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nickel/cobalt efflux system</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">hoxN</name>
        <name evidence="9" type="ordered locus">SSO1018</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the NiCoT transporter (TC 2.A.52) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90253">
        <property type="entry name" value="B90253"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZB2"/>
    <dbReference type="STRING" id="273057.SSO1018"/>
    <dbReference type="PaxDb" id="273057-SSO1018"/>
    <dbReference type="EnsemblBacteria" id="AAK41281">
        <property type="protein sequence ID" value="AAK41281"/>
        <property type="gene ID" value="SSO1018"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1018"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1009"/>
    <dbReference type="eggNOG" id="arCOG06023">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036094_2_1_2"/>
    <dbReference type="InParanoid" id="Q97ZB2"/>
    <dbReference type="PhylomeDB" id="Q97ZB2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044750">
        <property type="term" value="F:high-affinity nickel cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098716">
        <property type="term" value="P:nickel cation import across plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004688">
        <property type="entry name" value="Ni/Co_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011541">
        <property type="entry name" value="Ni/Co_transpt_high_affinity"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31611">
        <property type="entry name" value="HIGH-AFFINITY NICKEL TRANSPORT PROTEIN NIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31611:SF0">
        <property type="entry name" value="HIGH-AFFINITY NICKEL TRANSPORT PROTEIN NIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03824">
        <property type="entry name" value="NicO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0533">Nickel</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="223"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="297"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK41281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="373" mass="42460" checksum="8E8C26B58AADDBF6" modified="2001-10-01" version="1">MLQNSRKIYMMDRQSIYAMRSILNTLTQTISYKNLVTLSLFYVLNVLLTALLFLFLYTLPQKPLTLNTDSGELIGTFITLGVLSYTFGLRHAVDADHLAAIDNVTRKMLQEGKNPVFVGTFFSFGHSTVVILLSLAVMMASRLVTNSLPSLENTGNLIETLISGGFLYLLGLLNTIVFYELYSFYKEGKYDKQKLEEILQKRGFMNKYFNKLFKIITNQWQMYIVGFLFGLGFDTATEVAILSISAILSSTYIHIPIYIILLYPILFSLGMSLVDTTDGLFMRFAYGWAFLSPLRKIWYNLTITLISILIAFGIGTIELLGLLQAEFNLTGLFWDQIAALNNVYWETIGYYVILTFAITWIGSGMIYRIKKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZD7</accession>
    <name>Q97ZD7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12 13">Pyruvate kinase</fullName>
            <ecNumber evidence="3 12 13">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">pyK</name>
        <name evidence="16" type="ordered locus">SSO0981</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="3 12 13"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90249">
        <property type="entry name" value="H90249"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923158.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZD7"/>
    <dbReference type="SMR" id="Q97ZD7"/>
    <dbReference type="FunCoup" id="Q97ZD7">
        <property type="interactions" value="226"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0981"/>
    <dbReference type="PaxDb" id="273057-SSO0981"/>
    <dbReference type="EnsemblBacteria" id="AAK41255">
        <property type="protein sequence ID" value="AAK41255"/>
        <property type="gene ID" value="SSO0981"/>
    </dbReference>
    <dbReference type="GeneID" id="1455222"/>
    <dbReference type="GeneID" id="27427302"/>
    <dbReference type="KEGG" id="sso:SSO0981"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.980"/>
    <dbReference type="eggNOG" id="arCOG04120">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015439_0_2_2"/>
    <dbReference type="InParanoid" id="Q97ZD7"/>
    <dbReference type="PhylomeDB" id="Q97ZD7"/>
    <dbReference type="BRENDA" id="2.7.1.40">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 13 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 16" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="14">
        <location>
            <begin position="1"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="15">
        <location>
            <begin position="331"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR01064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AAK41255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="452" mass="49884" checksum="1856968BD88FB754" modified="2001-10-01" version="1">MRKTKIVATLGPSSEEKVKELAEYVDVFRINFAHGDETSHRKYFDLIRTYAPESSIIVDLPGPKLRLGELKEPIEVKKGDKIVFSQKDGIPVDDELFYSAVKENSDILIADGTIRVRVKSKAKDRVEGTVIEGGILLSRKGINIPNVNLKSGITDNDLKLLKRALDLGADYIGLSFVISENDVKKVKEFVGDEAWVIAKIEKSEALKNLTNIVNESDGIMVARGDLGVETGLENLPLIQRRIVRTSRVFGKPVILATQVLTSMINSPIPTRAEIIDISNSIMQGVDSIMLSDETAIGNYPVESVRTLHNIISNVEKSVKHRPIGPLNSESDAIALAAVNASKVSKADVIVVYSRSGNSILRVSRLRPERNIIGVSPDPRLAKKFKLCYGVIPISINKKMQSIDEIIDVSAKLMQEKIKDLKFKKIVIVGGDPKQEAGKTNFVIVKTLEQQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZI2</accession>
    <name>Q97ZI2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0925</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_000604_48_2_2"/>
    <dbReference type="InParanoid" id="Q97ZI2"/>
    <dbReference type="PhylomeDB" id="Q97ZI2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 6, CHLOROPLASTIC"/>
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    </dbReference>
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        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 6, CHLOROPLASTIC"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="19"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="162" mass="17770" checksum="A6A4C582D4B16463" modified="2001-10-01" version="1">MVTLKIEDLHVEVEGKEILRGINLEVRSGEIHALMGPNGSGKTSLSLAIMGHPKYKITKGRIVLDGEDITNLEPHEKAKKGIFLAFQNPIEIAGVRLSTLLVAEYNKVSDSSASVMEVISKVKNISKEVGIADSLLNRGVFEGFSWRRKEESRDLTNASIKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2025-06-18" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZL0</accession>
    <name>ACYP_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Acylphosphatase</fullName>
            <ecNumber>3.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Acylphosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">acyP</name>
        <name type="ordered locus">SSO0887</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="Proteins" volume="62" first="64" last="79">
            <title>Structure, conformational stability, and enzymatic properties of acylphosphatase from the hyperthermophile Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Corazza A."/>
                <person name="Rosano C."/>
                <person name="Pagano K."/>
                <person name="Alverdi V."/>
                <person name="Esposito G."/>
                <person name="Capanni C."/>
                <person name="Bemporad F."/>
                <person name="Plakoutsi G."/>
                <person name="Stefani M."/>
                <person name="Chiti F."/>
                <person name="Zuccotti S."/>
                <person name="Bolognesi M."/>
                <person name="Viglino P."/>
            </authorList>
            <dbReference type="PubMed" id="16287076"/>
            <dbReference type="DOI" id="10.1002/prot.20703"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.27 ANGSTROMS)</scope>
        <scope>STRUCTURE BY NMR</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>ACTIVE SITE</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>an acyl phosphate + H2O = a carboxylate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">0.36 mM for benzoylphosphate at 25 degrees Celsius</KM>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 4.7-5.7.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 81 degrees Celsius. Thermostable up to 100.8 degrees Celsius. Poorly active at 25 degrees Celsius. Enzymatic activity is increased 4-fold by temperature increase from 25 to 45 degrees Celsius and more than 9-fold at 98 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acylphosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="C90239"/>
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        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-101"/>
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    <dbReference type="PDB" id="2BJD">
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        <property type="resolution" value="1.27 A"/>
        <property type="chains" value="A/B=1-101"/>
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    <dbReference type="PDB" id="2BJE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A/C/E/G=1-101"/>
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    <dbReference type="PDB" id="4OIX">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.55 A"/>
        <property type="chains" value="A=1-101"/>
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    <dbReference type="PDB" id="4OJ1">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A/B=1-101"/>
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    <dbReference type="PDB" id="4OJ3">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B=1-101"/>
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    <dbReference type="PDB" id="4OJG">
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        <property type="resolution" value="1.38 A"/>
        <property type="chains" value="A/B=1-101"/>
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        <property type="resolution" value="1.60 A"/>
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    <dbReference type="PaxDb" id="273057-SSO0887"/>
    <dbReference type="EnsemblBacteria" id="AAK41170">
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    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0887"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.891"/>
    <dbReference type="eggNOG" id="arCOG01674">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141932_2_1_2"/>
    <dbReference type="InParanoid" id="Q97ZL0"/>
    <dbReference type="PhylomeDB" id="Q97ZL0"/>
    <dbReference type="BRENDA" id="3.6.1.7">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q97ZL0"/>
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    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="DisProt" id="DP00513"/>
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        <property type="entry name" value="Acylphosphatase"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Acylphosphatase_CS"/>
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        <property type="entry name" value="PRK14440.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ACYLPHOSPHATASE"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="Acylphosphatase/BLUF domain-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00151">
        <property type="entry name" value="ACYLPHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000326870" description="Acylphosphatase">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Acylphosphatase-like" evidence="1">
        <location>
            <begin position="15"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="25"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="50"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="54"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="96"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16287076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="1Y9O"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="2BJD"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="4OJ3"/>
        </source>
    </evidence>
    <sequence length="101" mass="11636" checksum="9BC4BF537EA7B4FA" modified="2001-10-01" version="1">MKKWSDTEVFEMLKRMYARVYGLVQGVGFRKFVQIHAIRLGIKGYAKNLPDGSVEVVAEGYEEALSKLLERIKQGPPAAEVEKVDYSFSEYKGEFEDFETY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZN6</accession>
    <name>Q97ZN6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0848</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90235">
        <property type="entry name" value="G90235"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923137.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZN6"/>
    <dbReference type="STRING" id="273057.SSO0848"/>
    <dbReference type="PaxDb" id="273057-SSO0848"/>
    <dbReference type="EnsemblBacteria" id="AAK41142">
        <property type="protein sequence ID" value="AAK41142"/>
        <property type="gene ID" value="SSO0848"/>
    </dbReference>
    <dbReference type="GeneID" id="1455109"/>
    <dbReference type="KEGG" id="sso:SSO0848"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.864"/>
    <dbReference type="eggNOG" id="arCOG07252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1485950_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZN6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="182" mass="20699" checksum="4B81BAFA77E1958E" modified="2001-10-01" version="1">MIIKSIRILFKFSYPYTLDLRDMIRILKDLSYVISSNLPLTPPGSMILGSVTALKDDLIVEFNNITGTISFFIQSLDKIDLVEEDFSRIVGNLNLGKIDYIELSLEMILNGKLNLNINMLGGEVVGLEVSSERKNVRINSYMAMANSYSVLITYKLNEIKDLDKVTEEIKRDIQELENKGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZP6</accession>
    <name>Q97ZP6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO6566</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90230">
        <property type="entry name" value="G90230"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZP6"/>
    <dbReference type="STRING" id="273057.SSO6566"/>
    <dbReference type="PaxDb" id="273057-SSO6566"/>
    <dbReference type="EnsemblBacteria" id="AAK41102">
        <property type="protein sequence ID" value="AAK41102"/>
        <property type="gene ID" value="SSO6566"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO6566"/>
    <dbReference type="HOGENOM" id="CLU_2327380_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZP6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="98" mass="11361" checksum="95165F3D48427D14" modified="2001-10-01" version="1">MRRWGLHIVRGIGGMRLSLGFLSTLGRKRRIRVYFARATNLDLPKGEVLRLYNKVRGPIFWGIKAFLPFTTKFVFRVLVFVLVLYSLYIQGDDGEGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q980E0</accession>
    <name>Q980E0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Heme biosynthesis related protein (NirJ-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nirJ-like</name>
        <name evidence="8" type="ordered locus">SSO0373</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90180">
        <property type="entry name" value="H90180"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990676.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980E0"/>
    <dbReference type="SMR" id="Q980E0"/>
    <dbReference type="STRING" id="273057.SSO0373"/>
    <dbReference type="PaxDb" id="273057-SSO0373"/>
    <dbReference type="EnsemblBacteria" id="AAK40703">
        <property type="protein sequence ID" value="AAK40703"/>
        <property type="gene ID" value="SSO0373"/>
    </dbReference>
    <dbReference type="GeneID" id="1455513"/>
    <dbReference type="KEGG" id="sso:SSO0373"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.367"/>
    <dbReference type="eggNOG" id="arCOG00938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_859484_0_0_2"/>
    <dbReference type="InParanoid" id="Q980E0"/>
    <dbReference type="PhylomeDB" id="Q980E0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000377">
        <property type="entry name" value="Radical SAM domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF34">
        <property type="entry name" value="TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE COFACTOR MODIFYING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="20"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK40703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="322" mass="37351" checksum="864CAAF65AF1968F" modified="2001-10-01" version="1">MNKDLKAIKWFITTQILKDPFNPAYATFKVTSRCNLRCTFCSPSYYSGELGEGSTERVKKIIDNLRDSSIVVLSFEGGEPTYRPDILDLLEYAHDGSFYIMLTTNGYRLKDEDFLTKLADKIDFLHYSIDEYHWNVKELENLCRFRKYGLKVNVQTVVTRYNLYKLEEKVRKVAECGYKILAMPAVDYPNSKVKLAPDPYEFYKVLYDLKRKYGSTLNNSWGFINAIIGKVPKRVVSYAITIYPNGDLPYPDDINGEIVGNVAEEPLNKILQSSKVKELQQKMLENQAKFEYLHLQTASFNNIRDLASYVSEMLRWRFTGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q980I1</accession>
    <name>Q980I1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0314</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90174">
        <property type="entry name" value="C90174"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980I1"/>
    <dbReference type="PaxDb" id="273057-SSO0314"/>
    <dbReference type="EnsemblBacteria" id="AAK40650">
        <property type="protein sequence ID" value="AAK40650"/>
        <property type="gene ID" value="SSO0314"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0314"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.307"/>
    <dbReference type="eggNOG" id="arCOG04235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095574_0_0_2"/>
    <dbReference type="InParanoid" id="Q980I1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin-like fold" evidence="2">
        <location>
            <begin position="11"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="274" mass="32241" checksum="97B642474C0CE8AE" modified="2001-10-01" version="1">MQGKLNCEIEMEVQIFTHKNCVECNMLLEYLENKGLLGKVKIIDTELYPFLAFEKGVISTPSIFIDGKLVYAGIVDFEEFERILSGQKVTKQIDKASLVEKLMLGIVDSFAATAWLYINRDFDSFLAQKDFVMAVTGLSLLDEREGDEYYNYLRNVMVKEGDRYLEEWKPRMLRNISSNFIREIFWLYERKISKETLTQKYPVEVFAHWLMIRSGAVGRVGLRIYPLSDSTVMKRVLEAYNFMLENYDEIFNKVQKEQMELKTKNKEQVRYLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q980J3</accession>
    <name>Q980J3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="5">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tkt-1</name>
        <name evidence="9" type="ordered locus">SSO0297</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90172">
        <property type="entry name" value="G90172"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980J3"/>
    <dbReference type="FunCoup" id="Q980J3">
        <property type="interactions" value="189"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0297"/>
    <dbReference type="PaxDb" id="273057-SSO0297"/>
    <dbReference type="EnsemblBacteria" id="AAK40638">
        <property type="protein sequence ID" value="AAK40638"/>
        <property type="gene ID" value="SSO0297"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0297"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.294"/>
    <dbReference type="eggNOG" id="arCOG01053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_4_1_2"/>
    <dbReference type="InParanoid" id="Q980J3"/>
    <dbReference type="PhylomeDB" id="Q980J3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47514">
        <property type="entry name" value="TRANSKETOLASE N-TERMINAL SECTION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47514:SF1">
        <property type="entry name" value="TRANSKETOLASE N-TERMINAL SECTION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transketolase N-terminal" evidence="8">
        <location>
            <begin position="45"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK40638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="281" mass="30940" checksum="F1DE17A20C58B2C1" modified="2001-10-01" version="1">MGIDIVPMEKGGGSDGTPISIEELDKLRQVAEKARRNVIKMLFYDQTIHVGSSLSSIEILTTLIFKHIRTDSSLVNKDWLILSKGHAAPALYAVLAEKGYIKEEELWRIQDITGLLQGHPETFIPGVDMSTGSLGQGLSFGIGVATGIKMANGTGRVYVIMGDGEQDEGEIWEAMTHAVVRNLDNLIAFIEMNNFQLDGSTDEIKPKNFLPKVWEAVGWKVLNCDGHDFISITNAVNEAYKASKPVVIFAKTVRGKGFPPIENTHKQRSSPDDARKYLLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q980K9</accession>
    <name>Q980K9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0284</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90170">
        <property type="entry name" value="G90170"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990566.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980K9"/>
    <dbReference type="STRING" id="273057.SSO0284"/>
    <dbReference type="PaxDb" id="273057-SSO0284"/>
    <dbReference type="EnsemblBacteria" id="AAK40622">
        <property type="protein sequence ID" value="AAK40622"/>
        <property type="gene ID" value="SSO0284"/>
    </dbReference>
    <dbReference type="GeneID" id="1455432"/>
    <dbReference type="KEGG" id="sso:SSO0284"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.278"/>
    <dbReference type="eggNOG" id="arCOG07188">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168021_0_0_2"/>
    <dbReference type="InParanoid" id="Q980K9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="121" mass="14056" checksum="722FEF054A9E0B3C" modified="2001-10-01" version="1">MDIANINNLIKHDDEYVSQVLPLLPSEGVLRQACFLYFNYDTSRNKIRSPIMFFLLLLLPFEQIKDALDYIRGEINSVNELYLIECIKNTSAQENFLPYQIMGNKERLILTKNALVLIDSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-08-10" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q980L4</accession>
    <name>PSB1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome subunit beta 1</fullName>
            <ecNumber evidence="1">3.4.25.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">20S proteasome beta subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Proteasome core protein PsmB 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">psmB1</name>
        <name type="ordered locus">SSO0278</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99170">
        <property type="entry name" value="A99170"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990557.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980L4"/>
    <dbReference type="SMR" id="Q980L4"/>
    <dbReference type="FunCoup" id="Q980L4">
        <property type="interactions" value="231"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0278"/>
    <dbReference type="PaxDb" id="273057-SSO0278"/>
    <dbReference type="EnsemblBacteria" id="AAK40616">
        <property type="protein sequence ID" value="AAK40616"/>
        <property type="gene ID" value="SSO0278"/>
    </dbReference>
    <dbReference type="GeneID" id="1455426"/>
    <dbReference type="KEGG" id="sso:SSO0278"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.272"/>
    <dbReference type="eggNOG" id="arCOG00970">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_7_2_2"/>
    <dbReference type="InParanoid" id="Q980L4"/>
    <dbReference type="PhylomeDB" id="Q980L4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000079">
        <property type="entry name" value="Proteasome subunit beta 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02113_A">
        <property type="entry name" value="Proteasome_B_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019983">
        <property type="entry name" value="Pept_T1A_Psome_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03634">
        <property type="entry name" value="arc_protsome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-0647">Proteasome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0888">Threonine protease</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_0000397454" description="Removed in mature form; by autocatalysis" evidence="1">
        <location>
            <begin position="1"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000397455" description="Proteasome subunit beta 1">
        <location>
            <begin position="7"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02113"/>
        </source>
    </evidence>
    <sequence length="196" mass="21376" checksum="506E2B6031B86DD5" modified="2001-10-01" version="1" precursor="true">MEELPATAVGLKVNDGIVLASERRLSYGGYVLSKQAKKVYKINKFLMAGAGIYGDLQTLTRIMNVEIKYYEVSTGKPISVHAAAKLLSVILYQYKVMPFISEILFGGVDEKGPQLYVLDPIGSLIEDNYAAVGSGARIAIGVLESEYDPNMSLDVATQLITKAIKASIERDITSGDGIDLAIIDKKGNYENKFIPY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-10" modified="2026-01-28" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q980M1</accession>
    <name>PAN_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome-activating nucleotidase</fullName>
            <shortName evidence="1">PAN</shortName>
            <ecNumber evidence="1">3.6.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Proteasomal ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Proteasome regulatory ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Proteasome regulatory particle</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pan</name>
        <name type="ordered locus">SSO0271</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90169">
        <property type="entry name" value="B90169"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990546.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980M1"/>
    <dbReference type="SMR" id="Q980M1"/>
    <dbReference type="FunCoup" id="Q980M1">
        <property type="interactions" value="266"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0271"/>
    <dbReference type="PaxDb" id="273057-SSO0271"/>
    <dbReference type="EnsemblBacteria" id="AAK40609">
        <property type="protein sequence ID" value="AAK40609"/>
        <property type="gene ID" value="SSO0271"/>
    </dbReference>
    <dbReference type="GeneID" id="1455419"/>
    <dbReference type="KEGG" id="sso:SSO0271"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.265"/>
    <dbReference type="eggNOG" id="arCOG01306">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_2_0_2"/>
    <dbReference type="InParanoid" id="Q980M1"/>
    <dbReference type="PhylomeDB" id="Q980M1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022623">
        <property type="term" value="C:proteasome-activating nucleotidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036402">
        <property type="term" value="F:proteasome-activating activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000033">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00553">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023501">
        <property type="entry name" value="Nucleotidase_PAN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003069">
        <property type="entry name" value="PRK03992.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01242">
        <property type="entry name" value="proteasome-activating nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0647">Proteasome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000084752" description="Proteasome-activating nucleotidase">
        <location>
            <begin position="1"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Docks into pockets in the proteasome alpha-ring to cause gate opening" evidence="1">
        <location>
            <begin position="391"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="15"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="365"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00553"/>
        </source>
    </evidence>
    <sequence length="393" mass="44142" checksum="05578B92D5041A83" modified="2001-10-01" version="1">MSGDFDTIRDASSPDEVQLVRLLEEKIKSLQIEIENLRKELNYYKAEMEKMLSPPLIEAVVLDVLPDGRVLVRSSSGPNLVVNIASHIDQKLIKPGISVALNQRGSTILEVLPQKEDPIVKTMEIIERPNVTYSEIGGLEEQIRELREVVELPLKNPEIFREIGVEPPKGVLLYGPPGTGKTMLAKAVATESNAVFIHVVASEFAQKFVGEGARIVRELFEMAKRKAPSIIFIDEIDAIGAKRIDIGTSGEREIQRTLMQLLAELDGFDPLDNVKIIAATNRIDILDPALLRPGRFDRIIEVPLPDFKGRTEIFNIYLKKMKIEDNINLELLSQLTEGFSGADIKNVCVEAAYMAIRDGRNKVTMNDLVEAINKINVKRNKMESMKERREKYS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-09-21" modified="2026-01-28" version="146" xmlns="https://uniprot.org/uniprot">
    <accession>Q980M3</accession>
    <name>HFLX_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTPase HflX</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GTP-binding protein HflX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hflX</name>
        <name type="ordered locus">SSO0269</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="J. Bacteriol." volume="193" first="2861" last="2867">
            <title>An HflX-Type GTPase from Sulfolobus solfataricus binds to the 50S ribosomal subunit in all nucleotide-bound states.</title>
            <authorList>
                <person name="Blombach F."/>
                <person name="Launay H."/>
                <person name="Zorraquino V."/>
                <person name="Swarts D.C."/>
                <person name="Cabrita L.D."/>
                <person name="Benelli D."/>
                <person name="Christodoulou J."/>
                <person name="Londei P."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="21478358"/>
            <dbReference type="DOI" id="10.1128/JB.01552-10"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH 50S SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="J. Biochem." volume="148" first="103" last="113">
            <title>Functional study on GTP hydrolysis by the GTP-binding protein from Sulfolobus solfataricus, a member of the HflX family.</title>
            <authorList>
                <person name="Huang B."/>
                <person name="Wu H."/>
                <person name="Hao N."/>
                <person name="Blombach F."/>
                <person name="van der Oost J."/>
                <person name="Li X."/>
                <person name="Zhang X.C."/>
                <person name="Rao Z."/>
            </authorList>
            <dbReference type="PubMed" id="20400571"/>
            <dbReference type="DOI" id="10.1093/jb/mvq039"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF WILD-TYPE IN COMPLEX WITH GDP AND OF MUTANTS PRO-235 AND SER-235</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>MUTAGENESIS OF ASN-189; THR-193; THR-213; GLY-235 AND PHE-236</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2010" name="Proteins" volume="78" first="705" last="713">
            <title>Structure of the ribosome associating GTPase HflX.</title>
            <authorList>
                <person name="Wu H."/>
                <person name="Sun L."/>
                <person name="Blombach F."/>
                <person name="Brouns S.J."/>
                <person name="Snijders A.P."/>
                <person name="Lorenzen K."/>
                <person name="van den Heuvel R.H."/>
                <person name="Heck A.J."/>
                <person name="Fu S."/>
                <person name="Li X."/>
                <person name="Zhang X.C."/>
                <person name="Rao Z."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="19787775"/>
            <dbReference type="DOI" id="10.1002/prot.22599"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH GDP</scope>
        <scope>FUNCTION AS A GTPASE</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 4">GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis. Specific for GTP.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="2 4">GTPase activity is stimulated by the presence of 50S ribosomal subunits. Hydrolysis is probably regulated by the HflX N-terminal domain.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">5.3 uM for GTP</KM>
        </kinetics>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3 4">Monomer. Associates with the 50S ribosomal subunit. Does not associate with 70S ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">May associate with membranes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. HflX GTPase family.</text>
    </comment>
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        <property type="gene ID" value="SSO0269"/>
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    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000412528" description="GTPase HflX">
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            <end position="356"/>
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    <feature type="domain" description="Hflx-type G" evidence="1">
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    </feature>
    <feature type="binding site" evidence="1 5 6">
        <location>
            <begin position="186"/>
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        <ligand>
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    </feature>
    <feature type="binding site" evidence="1">
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        <ligand>
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    <feature type="binding site" evidence="1">
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        <ligand>
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    <feature type="mutagenesis site" description="Loss of GTPase activity." evidence="3">
        <original>N</original>
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    <feature type="mutagenesis site" description="Loss of GTPase activity." evidence="3">
        <original>T</original>
        <variation>N</variation>
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            <position position="193"/>
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    </feature>
    <feature type="mutagenesis site" description="Decrease in GTPase activity." evidence="3">
        <original>T</original>
        <variation>V</variation>
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of GTPase activity." evidence="3">
        <original>G</original>
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        <location>
            <position position="235"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Decrease in GTPase activity." evidence="3">
        <original>G</original>
        <variation>S</variation>
        <location>
            <position position="235"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Increase in KM for GTP and in GTPase activity." evidence="3">
        <original>F</original>
        <variation>P</variation>
        <location>
            <position position="236"/>
        </location>
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        <location>
            <begin position="2"/>
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            <begin position="10"/>
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    <feature type="helix" evidence="7">
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    <feature type="turn" evidence="7">
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    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="341"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00900"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="21478358"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6">
        <source>
            <dbReference type="PubMed" id="20400571"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="2QTF"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="3KXK"/>
        </source>
    </evidence>
    <sequence length="356" mass="40538" checksum="602D4DBBE3BEAB1C" modified="2001-10-01" version="1">MKTAALFVSKEFEEEAIALVEGANYKVTSIYKLPKSPNVKFYIQYDKLQQIKNDEEISTLIIFEQLKPRHFINIRRELKGKEVLDKILLLLEIFALHAGSKEAKMQIELARLKYELPIIKETYTKSKIGEQQGPLGAGTYGVESTIKFYKRRINKLMKELESIKIFKEKSIESNKRNNIPSIGIVGYTNSGKTSLFNSLTGLTQKVDTKLFTTMSPKRYAIPINNRKIMLVDTVGFIRGIPPQIVDAFFVTLSEAKYSDALILVIDSTFSENLLIETLQSSFEILREIGVSGKPILVTLNKIDKINGDLYKKLDLVEKLSKELYSPIFDVIPISALKRTNLELLRDKIYQLATQLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2026-01-28" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>Q980M5</accession>
    <name>TFE_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Transcription factor E</fullName>
            <shortName>TFE</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>TFIIE subunit alpha homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Transcription initiation factor TFIIE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">tfe</name>
        <name type="ordered locus">SSO0266</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="EMBO Rep." volume="2" first="133" last="138">
            <title>The archaeal TFIIEalpha homologue facilitates transcription initiation by enhancing TATA-box recognition.</title>
            <authorList>
                <person name="Bell S.D."/>
                <person name="Brinkman A.B."/>
                <person name="van der Oost J."/>
                <person name="Jackson S.P."/>
            </authorList>
            <dbReference type="PubMed" id="11258705"/>
            <dbReference type="DOI" id="10.1093/embo-reports/kve021"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH TBP AND RNA POLYMERASE</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="J. Biol. Chem." volume="278" first="48267" last="48274">
            <title>An extended winged helix domain in general transcription factor E/IIE alpha.</title>
            <authorList>
                <person name="Meinhart A."/>
                <person name="Blobel J."/>
                <person name="Cramer P."/>
            </authorList>
            <dbReference type="PubMed" id="13679366"/>
            <dbReference type="DOI" id="10.1074/jbc.M307874200"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 1-110</scope>
        <scope>DOMAIN</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 3">Monomer (Probable). Interaction with RNA polymerase subunits RpoF and RpoE is necessary for Tfe stimulatory transcription activity. Able to interact with Tbp and RNA polymerase in the absence of DNA promoter. Interacts both with the preinitiation and elongation complexes.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The winged helix domain is involved in binding to DNA in the preinitiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TFE family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90168">
        <property type="entry name" value="F90168"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990539.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1Q1H">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="A=1-110"/>
    </dbReference>
    <dbReference type="PDBsum" id="1Q1H"/>
    <dbReference type="AlphaFoldDB" id="Q980M5"/>
    <dbReference type="SMR" id="Q980M5"/>
    <dbReference type="STRING" id="273057.SSO0266"/>
    <dbReference type="PaxDb" id="273057-SSO0266"/>
    <dbReference type="EnsemblBacteria" id="AAK40605">
        <property type="protein sequence ID" value="AAK40605"/>
        <property type="gene ID" value="SSO0266"/>
    </dbReference>
    <dbReference type="GeneID" id="1455415"/>
    <dbReference type="GeneID" id="44129239"/>
    <dbReference type="KEGG" id="sso:SSO0266"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.261"/>
    <dbReference type="eggNOG" id="arCOG04270">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100097_0_0_2"/>
    <dbReference type="InParanoid" id="Q980M5"/>
    <dbReference type="PhylomeDB" id="Q980M5"/>
    <dbReference type="EvolutionaryTrace" id="Q980M5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01909">
        <property type="entry name" value="TFE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016481">
        <property type="entry name" value="TF_E_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF7">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006373">
        <property type="entry name" value="TF_E_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000318948" description="Transcription factor E">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="HTH TFE/IIEalpha-type">
        <location>
            <begin position="4"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="3"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="7"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="36"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="64"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="77"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="84"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11258705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="1Q1H"/>
        </source>
    </evidence>
    <sequence length="178" mass="21173" checksum="F7A734DA4030D40F" modified="2001-10-01" version="1">MVNAEDLFINLAKSLLGDDVIDVLRILLDKGTEMTDEEIANQLNIKVNDVRKKLNLLEEQGFVSYRKTRDKDSGWFIYYWKPNIDQINEILLNRKRLILDKLKTRLEYEKNNTFFICPQDNSRYSFEEAFENEFKCLKCGSQLTYYDTDKIKSFLEQKIRQIEEEIDKETKLGANKNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q980N0</accession>
    <name>Q980N0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription elongation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90168">
        <property type="entry name" value="A90168"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990531.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980N0"/>
    <dbReference type="STRING" id="273057.SSO0260"/>
    <dbReference type="PaxDb" id="273057-SSO0260"/>
    <dbReference type="EnsemblBacteria" id="AAK40600">
        <property type="protein sequence ID" value="AAK40600"/>
        <property type="gene ID" value="SSO0260"/>
    </dbReference>
    <dbReference type="GeneID" id="1455410"/>
    <dbReference type="KEGG" id="sso:SSO0260"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.256"/>
    <dbReference type="eggNOG" id="arCOG04136">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165872_0_0_2"/>
    <dbReference type="InParanoid" id="Q980N0"/>
    <dbReference type="PhylomeDB" id="Q980N0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007808">
        <property type="entry name" value="Elf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038567">
        <property type="entry name" value="T_Elf1_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011482">
        <property type="entry name" value="PRK14892.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934:SF0">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="12251" checksum="E317E327A50B465F" modified="2001-10-01" version="1">MGGKRRKRTKIIRVKPKLPKTFECPRCGKVSVSVKIKDNIATITCGSCGLYTEVEVPPVFDEANAYSKFIDMYLEGRLQIKERTNENTEEENEIEGESEEISHRQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q980N8</accession>
    <name>Q980N8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Proliferating-cell nucleolar antigen p120 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO0252</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90166">
        <property type="entry name" value="H90166"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990515.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980N8"/>
    <dbReference type="FunCoup" id="Q980N8">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0252"/>
    <dbReference type="PaxDb" id="273057-SSO0252"/>
    <dbReference type="EnsemblBacteria" id="AAK40591">
        <property type="protein sequence ID" value="AAK40591"/>
        <property type="gene ID" value="SSO0252"/>
    </dbReference>
    <dbReference type="GeneID" id="1455401"/>
    <dbReference type="KEGG" id="sso:SSO0252"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.246"/>
    <dbReference type="eggNOG" id="arCOG00973">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_7_0_2"/>
    <dbReference type="InParanoid" id="Q980N8"/>
    <dbReference type="PhylomeDB" id="Q980N8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016428">
        <property type="term" value="F:tRNA (cytidine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1170:FF:000019">
        <property type="entry name" value="RNA methylase, NOL1/NOP2/sun family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031341">
        <property type="entry name" value="Methyltr_RsmF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF74">
        <property type="entry name" value="TRNA (CYTOSINE(48)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17125">
        <property type="entry name" value="Methyltr_RsmF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="7">
        <location>
            <begin position="54"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK40591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="339" mass="38851" checksum="971DE3993608F2CE" modified="2001-10-01" version="1">MENRIAKYLSAHYNLFQISPSYKAKRLASKYRFLDYMIERYLNMFSTEEELEEYLNSCSFPLKKSIRCNTLKTDCKKLEEIMLEKGFVLEKVKWLQHGYVVKRTPPKPSLGSTLEYLMGYYHIQGLASMVPAYVLNPSYDDFVLDMAAAPGGKTTQLSQIMQNKGLVIAVEKKRSRIRALLSNVNRLGAENVVLIKTDALNLININKSQFDKILLDAPCSGEGLIQKDPTRRYKTTMDDLRDFAHLQLSLIEIAYELLKKGGYIVYSTCSVAPEEDELIVNFAIEELGMKAMSVEGYPASNGYIEYYTTRFSNDVKNCLRFFPHKQGTEGFFLCLLKKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-03-27" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q980S9</accession>
    <name>METK_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-adenosylmethionine synthase</fullName>
            <shortName evidence="1">AdoMet synthase</shortName>
            <ecNumber evidence="1">2.5.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Methionine adenosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mat</name>
        <name type="ordered locus">SSO0199</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AdoMet synthase 2 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40544" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40544.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90161">
        <property type="entry name" value="A90161"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990439.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="4HPV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.21 A"/>
        <property type="chains" value="A/B=1-404"/>
    </dbReference>
    <dbReference type="PDB" id="4K0B">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.39 A"/>
        <property type="chains" value="A/B=1-404"/>
    </dbReference>
    <dbReference type="PDB" id="4L2Z">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.49 A"/>
        <property type="chains" value="A/B=1-404"/>
    </dbReference>
    <dbReference type="PDB" id="4L7I">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.19 A"/>
        <property type="chains" value="A/B=1-404"/>
    </dbReference>
    <dbReference type="PDB" id="4WS9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-404"/>
    </dbReference>
    <dbReference type="PDBsum" id="4HPV"/>
    <dbReference type="PDBsum" id="4K0B"/>
    <dbReference type="PDBsum" id="4L2Z"/>
    <dbReference type="PDBsum" id="4L7I"/>
    <dbReference type="PDBsum" id="4WS9"/>
    <dbReference type="AlphaFoldDB" id="Q980S9"/>
    <dbReference type="SMR" id="Q980S9"/>
    <dbReference type="FunCoup" id="Q980S9">
        <property type="interactions" value="197"/>
    </dbReference>
    <dbReference type="MINT" id="Q980S9"/>
    <dbReference type="STRING" id="273057.SSO0199"/>
    <dbReference type="PaxDb" id="273057-SSO0199"/>
    <dbReference type="EnsemblBacteria" id="AAK40544">
        <property type="protein sequence ID" value="AAK40544"/>
        <property type="gene ID" value="SSO0199"/>
    </dbReference>
    <dbReference type="GeneID" id="1455354"/>
    <dbReference type="KEGG" id="sso:SSO0199"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.198"/>
    <dbReference type="eggNOG" id="arCOG01678">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057642_0_0_2"/>
    <dbReference type="InParanoid" id="Q980S9"/>
    <dbReference type="PhylomeDB" id="Q980S9"/>
    <dbReference type="BRENDA" id="2.5.1.6">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q980S9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.280">
        <property type="entry name" value="S-adenosylmethionine synthetase, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00136">
        <property type="entry name" value="S_AdoMet_synth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027790">
        <property type="entry name" value="AdoMet_synthase_2_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042544">
        <property type="entry name" value="AdoMet_synthase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002795">
        <property type="entry name" value="S-AdoMet_synthetase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003365">
        <property type="entry name" value="PRK04439.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003366">
        <property type="entry name" value="PRK04439.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36697">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36697:SF1">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01941">
        <property type="entry name" value="AdoMet_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000150041" description="S-adenosylmethionine synthase">
        <location>
            <begin position="1"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="139"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="4"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="10"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="14"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="17"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="59"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="70"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="77"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="84"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="95"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="99"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="107"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="128"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="131"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
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    <evidence type="ECO:0000255" key="1">
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    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="4HPV"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="4L7I"/>
        </source>
    </evidence>
    <sequence length="404" mass="44663" checksum="F6BD144BC36861C4" modified="2002-03-27" version="2">MRNINVQLNPLSDIEKLQVELVERKGLGHPDYIADAVAEEASRKLSLYYLKKYGVILHHNLDKTLVVGGQATPRFKGGDIIQPIYIIVAGRATTEVKTESGIDQIPVGTIIIESVKEWIRNNFRYLDAERHVIVDYKIGKGSSDLVGIFEASKRVPLSNDTSFGVGFAPLTKLEKLVYETERHLNSKQFKAKLPEVGEDIKVMGLRRGNEVDLTIAMATISELIEDVNHYINVKEQVRNQILDLASKIAPGYNVRVYVNTGDKIDKNILYLTVTGTSAEHGDDGMTGRGNRGVGLITPMRPMSLEATAGKNPVNHVGKLYNVLANLIANKIAQEVKDVKFSQVQVLGQIGRPIDDPLIANVDVITYDGKLTDETKNEISGIVDEMLSSFNKLTELILEGKATLF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-28" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q980V1</accession>
    <name>PTH_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidyl-tRNA hydrolase</fullName>
            <shortName evidence="1">PTH</shortName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pth</name>
        <name type="ordered locus">SSO0175</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="3227" last="3235">
            <title>Peptidyl-tRNA hydrolase from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Fromant M."/>
                <person name="Ferri-Fioni M.-L."/>
                <person name="Plateau P."/>
                <person name="Blanquet S."/>
            </authorList>
            <dbReference type="PubMed" id="12799450"/>
            <dbReference type="DOI" id="10.1093/nar/gkg428"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-10</scope>
        <scope>FUNCTION</scope>
        <scope>CHARACTERIZATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text>Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PTH2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90158">
        <property type="entry name" value="B90158"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990400.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="1XTY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B/C/D=1-120"/>
    </dbReference>
    <dbReference type="PDBsum" id="1XTY"/>
    <dbReference type="AlphaFoldDB" id="Q980V1"/>
    <dbReference type="SMR" id="Q980V1"/>
    <dbReference type="FunCoup" id="Q980V1">
        <property type="interactions" value="239"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0175"/>
    <dbReference type="PaxDb" id="273057-SSO0175"/>
    <dbReference type="EnsemblBacteria" id="AAK40521">
        <property type="protein sequence ID" value="AAK40521"/>
        <property type="gene ID" value="SSO0175"/>
    </dbReference>
    <dbReference type="GeneID" id="1453348"/>
    <dbReference type="KEGG" id="sso:SSO0175"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.171"/>
    <dbReference type="eggNOG" id="arCOG04228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073661_2_2_2"/>
    <dbReference type="InParanoid" id="Q980V1"/>
    <dbReference type="PhylomeDB" id="Q980V1"/>
    <dbReference type="BRENDA" id="3.1.1.29">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q980V1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000001">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00628">
        <property type="entry name" value="Pept_tRNA_hydro_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034759">
        <property type="entry name" value="Pept_tRNA_hydro_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003314">
        <property type="entry name" value="PRK04322.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF11">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000120304" description="Peptidyl-tRNA hydrolase">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="2"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="56"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="81"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="88"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="107"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12799450"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1XTY"/>
        </source>
    </evidence>
    <sequence length="120" mass="13140" checksum="A90F6A3ADC714B91" modified="2001-10-01" version="1">MIKMVIVVRSDIKMGKGKIAAQVAHAAVTLVVSIINSNNLRWKEWLNEWLHQGQPKIIVKVNSLDEIISRAKKAETMNLPFSIIEDAGKTQLEPGTITCLGIGPAPENLVDSITGDLKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Y2</accession>
    <name>Q980Y2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Primase (Carboxy-end)</fullName>
            <ecNumber evidence="6">2.7.7.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0140</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.-" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90154">
        <property type="entry name" value="C90154"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922898.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Y2"/>
    <dbReference type="STRING" id="273057.SSO0140"/>
    <dbReference type="PaxDb" id="273057-SSO0140"/>
    <dbReference type="EnsemblBacteria" id="AAK40490">
        <property type="protein sequence ID" value="AAK40490"/>
        <property type="gene ID" value="SSO0140"/>
    </dbReference>
    <dbReference type="GeneID" id="1453317"/>
    <dbReference type="KEGG" id="sso:SSO0140"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.131"/>
    <dbReference type="eggNOG" id="arCOG06914">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_511565_0_0_2"/>
    <dbReference type="InParanoid" id="Q980Y2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004968">
        <property type="entry name" value="DNA_primase/NTPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006500">
        <property type="entry name" value="Helicase_put_C_phage/plasmid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014015">
        <property type="entry name" value="Helicase_SF3_DNA-vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045455">
        <property type="entry name" value="NrS-1_pol-like_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051620">
        <property type="entry name" value="ORF904-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014818">
        <property type="entry name" value="Phage/plasmid_primase_P4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01613">
        <property type="entry name" value="primase_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35372">
        <property type="entry name" value="ATP BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35372:SF2">
        <property type="entry name" value="SF3 HELICASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08706">
        <property type="entry name" value="D5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19263">
        <property type="entry name" value="DUF5906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03288">
        <property type="entry name" value="Pox_D5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00885">
        <property type="entry name" value="D5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51206">
        <property type="entry name" value="SF3_HELICASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SF3 helicase" evidence="5">
        <location>
            <begin position="172"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="532" mass="61911" checksum="70B2DA2BF393E170" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q981B8</accession>
    <name>Q981B8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acetoin utilization protein AcuC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">SSO0028</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Ketone degradation; acetoin degradation.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90142">
        <property type="entry name" value="C90142"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989612.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981B8"/>
    <dbReference type="FunCoup" id="Q981B8">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0028"/>
    <dbReference type="PaxDb" id="273057-SSO0028"/>
    <dbReference type="EnsemblBacteria" id="AAK40394">
        <property type="protein sequence ID" value="AAK40394"/>
        <property type="gene ID" value="SSO0028"/>
    </dbReference>
    <dbReference type="GeneID" id="1455285"/>
    <dbReference type="KEGG" id="sso:SSO0028"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.30"/>
    <dbReference type="eggNOG" id="arCOG00324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_8_0_2"/>
    <dbReference type="InParanoid" id="Q981B8"/>
    <dbReference type="PhylomeDB" id="Q981B8"/>
    <dbReference type="UniPathway" id="UPA00040"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045150">
        <property type="term" value="P:acetoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09994">
        <property type="entry name" value="HDAC_AcuC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003085">
        <property type="entry name" value="AcuC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0006">Acetoin catabolism</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="5">
        <location>
            <begin position="21"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="351" mass="39389" checksum="D24B14CE052B1170" modified="2001-10-01" version="1">MHKTAFIWTDEYYNYSFPDYHPFKSLRESMTKRLLEERGAFHFITLVEPKSIPEEALQLVHSKEYIEFVKYKSKEGQGYLDDGDTPAFKGIYEAALIRVSGSVKALELIKSGEFNHTINIGGGFHHAKRNRAAGFCVFNDVALISKLGESFFSRIAIVDIDGHHADGTQELLIDDNNILKISLHMFHPNFFPGTGDVNEIGLGKGEGYTINIPLPPGTGDDGYLLAFDEIVVPAIERYKPELIILVAGGDSHFNDPLVELKLSTHGYLDVVTKIHRLVHEYSNGRLIMLGGGGYNYDATARIWTISIAEIAGIYDLEYETLHDSFSTKSSQFVMEKIRNTISLIKKIHSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q981C1</accession>
    <name>Q981C1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidate cytidylyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90141">
        <property type="entry name" value="H90141"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989615.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q981C1"/>
    <dbReference type="STRING" id="273057.SSO0024"/>
    <dbReference type="PaxDb" id="273057-SSO0024"/>
    <dbReference type="DNASU" id="1455282"/>
    <dbReference type="EnsemblBacteria" id="AAK40391">
        <property type="protein sequence ID" value="AAK40391"/>
        <property type="gene ID" value="SSO0024"/>
    </dbReference>
    <dbReference type="GeneID" id="1455282"/>
    <dbReference type="KEGG" id="sso:SSO0024"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.27"/>
    <dbReference type="eggNOG" id="arCOG01879">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1280846_0_0_2"/>
    <dbReference type="InParanoid" id="Q981C1"/>
    <dbReference type="PhylomeDB" id="Q981C1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="24138" checksum="6B6562608F0DEE73" modified="2001-10-01" version="1">MLITISDIAWGIALSIWVAIVVLYISKLISKKFGAYVTRKAIHMLGGGIVAVLSPFVFNSPLVPIITSYLIMAYLITIRVLRKEMSWFMDKENLGEVFFAFSFGTVLLVMFILDPNYWTSTDLIYVAVLPLIFMSFGDGITGIIRNFVYKRRVKGLWGSMGMLIFCATVGYFVLNIPGLIAGILATVAELLPYIDDNISVPFLSFLFLYLFVRVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="150" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX08</accession>
    <name>PYRB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate carbamoyltransferase catalytic subunit</fullName>
            <ecNumber evidence="1">2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartate transcarbamylase</fullName>
            <shortName evidence="1">ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrB</name>
        <name type="ordered locus">SSO0614</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of carbamoyl phosphate and aspartate to form carbamoyl aspartate and inorganic phosphate, the committed step in the de novo pyrimidine nucleotide biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterooligomer of catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90208">
        <property type="entry name" value="F90208"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991143.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX08"/>
    <dbReference type="SMR" id="Q9UX08"/>
    <dbReference type="FunCoup" id="Q9UX08">
        <property type="interactions" value="270"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0614"/>
    <dbReference type="PaxDb" id="273057-SSO0614"/>
    <dbReference type="EnsemblBacteria" id="AAK40925">
        <property type="protein sequence ID" value="AAK40925"/>
        <property type="gene ID" value="SSO0614"/>
    </dbReference>
    <dbReference type="GeneID" id="1454892"/>
    <dbReference type="GeneID" id="44129616"/>
    <dbReference type="KEGG" id="sso:SSO0614"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.622"/>
    <dbReference type="eggNOG" id="arCOG00911">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_1_2_2"/>
    <dbReference type="InParanoid" id="Q9UX08"/>
    <dbReference type="PhylomeDB" id="Q9UX08"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000021">
        <property type="entry name" value="Aspartate carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002032">
        <property type="entry name" value="PRK00856.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF6">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00101">
        <property type="entry name" value="ATCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000113259" description="Aspartate carbamoyltransferase catalytic subunit">
        <location>
            <begin position="1"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="303" mass="34738" checksum="11738155AFD16070" modified="2000-05-01" version="1">MRLRHVVSSLDLTRDDYFRIFELADKFYDVKKLNYLSGKVVSLAFFEPSTRTAQSFHTAAIKLGADVIGFASEESTSIAKGENLADTIRMLNNYSNCIVMRHKFDGAALFASEISDIPIINAGDGKHEHPTQAVIDLYTVYKAFGEIDGRTFALLGDLKYARTVNSLLRALTRFKPKKVFLISPSQLKVRREILDELNYPVIETENPYDVIQEIDVLYVTRIQKERFVDEVEYEKVKESYVVDLKLVNMMKKDGIILHPLPRVTEIDRRVDKTVNAKYFYQASLAVPVRMALFYEVLGDREDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-07-11" modified="2026-01-28" version="146" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX21</accession>
    <name>PURQ_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit PurQ</fullName>
            <shortName evidence="1">FGAM synthase</shortName>
            <ecNumber evidence="1">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formylglycinamide ribonucleotide amidotransferase subunit I</fullName>
            <shortName evidence="1">FGAR amidotransferase I</shortName>
            <shortName evidence="1">FGAR-AT I</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutaminase PurQ</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purQ</name>
        <name type="ordered locus">SSO0628</name>
        <name type="ORF">C08_022</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90210">
        <property type="entry name" value="D90210"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991164.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX21"/>
    <dbReference type="SMR" id="Q9UX21"/>
    <dbReference type="FunCoup" id="Q9UX21">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0628"/>
    <dbReference type="PaxDb" id="273057-SSO0628"/>
    <dbReference type="EnsemblBacteria" id="AAK40939">
        <property type="protein sequence ID" value="AAK40939"/>
        <property type="gene ID" value="SSO0628"/>
    </dbReference>
    <dbReference type="GeneID" id="1454906"/>
    <dbReference type="GeneID" id="44129630"/>
    <dbReference type="KEGG" id="sso:SSO0628"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.635"/>
    <dbReference type="eggNOG" id="arCOG00102">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001031_3_1_2"/>
    <dbReference type="InParanoid" id="Q9UX21"/>
    <dbReference type="PhylomeDB" id="Q9UX21"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01740">
        <property type="entry name" value="GATase1_FGAR_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00421">
        <property type="entry name" value="PurQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010075">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurQ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01737">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002957">
        <property type="entry name" value="PRK03619.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47552">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47552:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13507">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001586">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000100619" description="Phosphoribosylformylglycinamidine synthase subunit PurQ">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00421"/>
        </source>
    </evidence>
    <sequence length="224" mass="24686" checksum="8D8C25A34ED62D76" modified="2000-05-01" version="1">MIAIIKFPGTTCETDVYKALIEAGVPTVIVKYKDFDPDRYNGVILPGGFSFGDYLRAGSIAASTETMKKVKQMAEDGKIVIGICNGFQILVESGLLKGALLPNLKLRFISKWVYLKVIRADTILTKGLDKKIIRMPIAHAEGRYYVDDIDYAKTHMVLQYCDENGNISEDVNPNGSLLNIASIANEEGNVIGMMPHPERASFKLTSIDGTVDGLILLRRLGEWA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX29</accession>
    <name>PUR5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="1">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purM</name>
        <name type="ordered locus">SSO0636</name>
        <name type="ORF">C08_014</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIR synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99211">
        <property type="entry name" value="A99211"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991170.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX29"/>
    <dbReference type="SMR" id="Q9UX29"/>
    <dbReference type="FunCoup" id="Q9UX29">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0636"/>
    <dbReference type="PaxDb" id="273057-SSO0636"/>
    <dbReference type="EnsemblBacteria" id="AAK40944">
        <property type="protein sequence ID" value="AAK40944"/>
        <property type="gene ID" value="SSO0636"/>
    </dbReference>
    <dbReference type="GeneID" id="1454911"/>
    <dbReference type="GeneID" id="44129635"/>
    <dbReference type="KEGG" id="sso:SSO0636"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.640"/>
    <dbReference type="eggNOG" id="arCOG00639">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047116_0_0_2"/>
    <dbReference type="InParanoid" id="Q9UX29"/>
    <dbReference type="PhylomeDB" id="Q9UX29"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000020">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000011">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520:SF12">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148289" description="Phosphoribosylformylglycinamidine cyclo-ligase">
        <location>
            <begin position="1"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00741"/>
        </source>
    </evidence>
    <sequence length="323" mass="35400" checksum="17202798E4C5A401" modified="2000-05-01" version="1">MVSEEYKKAGVDLEKLRNYHNMISQIISSTYKNTIIGAGHYSGVIKIGSLNIAMHTDGVGTKTFLALQTRIIKPVGIDCVAMNVNDLICVGAKPVALVDYIALERPMDNVVNEIIDGIVQGAKEADVEVIGGETAIMPDVIRGFDLSCTAIGVVDKLKTGAEIRPGDYVLGLESSGIHANGYSLVRKLIEEGKLSLDEYKNELLKPTKIYVKPILEVMNMIKGAAHVTGGAFSKLKRLTSYKIVLNMPDPPQIFKTIEKAGVAHEEMYKVFNMGIGIVLFVSEELMKEVKTKLEGYGTVYELGRVYNGNGITIKTYKNEILRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX86</accession>
    <name>RL30_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL30</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L30</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl30</name>
        <name type="synonym">rpl30Ab</name>
        <name type="ordered locus">SSO0697</name>
        <name type="ORF">C10_033</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90217">
        <property type="entry name" value="G90217"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991253.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX86"/>
    <dbReference type="SMR" id="Q9UX86"/>
    <dbReference type="FunCoup" id="Q9UX86">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0697"/>
    <dbReference type="PaxDb" id="273057-SSO0697"/>
    <dbReference type="EnsemblBacteria" id="AAK40998">
        <property type="protein sequence ID" value="AAK40998"/>
        <property type="gene ID" value="SSO0697"/>
    </dbReference>
    <dbReference type="GeneID" id="1454965"/>
    <dbReference type="KEGG" id="sso:SSO0697"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.697"/>
    <dbReference type="eggNOG" id="arCOG04086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055156_6_0_2"/>
    <dbReference type="InParanoid" id="Q9UX86"/>
    <dbReference type="PhylomeDB" id="Q9UX86"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.15.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01371_A">
        <property type="entry name" value="Ribosomal_uL30_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005997">
        <property type="entry name" value="Ribosomal_uL30_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004711">
        <property type="entry name" value="PRK06049.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01309">
        <property type="entry name" value="uL30_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000104631" description="Large ribosomal subunit protein uL30">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="158" mass="18596" checksum="8E7051574A650A90" modified="2000-05-01" version="1">MVELLGIIRIRGWAKAPWYINETLNMLRLRYNFNTMVYPKTSQILGMLNKVSPYITWGEIDPDTLKLLIINRLETVKGDKVSDSYVKEVLKIENIDAMVKQLYEGKIYLHKLDEYFKLPIRLHPPRGGFKGSVKRPYKNKGEFGYRGDKINELMRRMV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>O33776</accession>
    <name>HIS2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Phosphoribosyl-ATP pyrophosphatase</fullName>
            <shortName>PRA-PH</shortName>
            <ecNumber>3.6.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hisE</name>
        <name type="ordered locus">SSO6223</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="J. Bacteriol." volume="179" first="4429" last="4432">
            <title>Evolutionary analysis of the hisCGABdFDEHI gene cluster from the archaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Sensen C.W."/>
                <person name="Doolittle W.F."/>
                <person name="Brown J.R."/>
            </authorList>
            <dbReference type="PubMed" id="9209067"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRA-PH family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40910" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.6.1.31"/>
    <dbReference type="EMBL" id="U82227">
        <property type="protein sequence ID" value="AAB63024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40910.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90206">
        <property type="entry name" value="G90206"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991118.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O33776"/>
    <dbReference type="SMR" id="O33776"/>
    <dbReference type="FunCoup" id="O33776">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO6223"/>
    <dbReference type="PaxDb" id="273057-SSO6223"/>
    <dbReference type="EnsemblBacteria" id="AAK40910">
        <property type="protein sequence ID" value="AAK40910"/>
        <property type="gene ID" value="SSO6223"/>
    </dbReference>
    <dbReference type="GeneID" id="1454877"/>
    <dbReference type="GeneID" id="44129600"/>
    <dbReference type="KEGG" id="sso:SSO6223"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.606"/>
    <dbReference type="eggNOG" id="arCOG02677">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123337_1_1_2"/>
    <dbReference type="InParanoid" id="O33776"/>
    <dbReference type="PhylomeDB" id="O33776"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11534">
        <property type="entry name" value="NTP-PPase_HisIE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1080">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01020">
        <property type="entry name" value="HisE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945:SF1">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101386">
        <property type="entry name" value="all-alpha NTP pyrophosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000136398" description="Phosphoribosyl-ATP pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="94" mass="10853" checksum="CBD50DBE2C9382E6" modified="2005-09-27" version="3">MSNEIVDELYKIILDRIEKRPTGSYTAEIVNKGKPYVARKVGEESVETIVASLAENKERFISEVADLIYHLLVLMALEGVTPEDIYRELERRRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-08-29" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>P95957</accession>
    <name>Y104_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized aminotransferase SSO0104</fullName>
            <ecNumber>2.6.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>AspB-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0104</name>
        <name type="ORF">C04018</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Mol. Microbiol." volume="22" first="175" last="191">
            <title>Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Sensen C.W."/>
                <person name="Klenk H.P."/>
                <person name="Singh R.K."/>
                <person name="Allard G."/>
                <person name="Chan C.C."/>
                <person name="Liu Q.Y."/>
                <person name="Penny S.L."/>
                <person name="Young F."/>
                <person name="Schenk M.E."/>
                <person name="Gaasterland T."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Charlebois R.L."/>
            </authorList>
            <dbReference type="PubMed" id="8899719"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.1996.tb02666.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-"/>
    <dbReference type="EMBL" id="Y08257">
        <property type="protein sequence ID" value="CAA69547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S75385">
        <property type="entry name" value="S75385"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988922.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P95957"/>
    <dbReference type="SMR" id="P95957"/>
    <dbReference type="FunCoup" id="P95957">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0104"/>
    <dbReference type="PaxDb" id="273057-SSO0104"/>
    <dbReference type="EnsemblBacteria" id="AAK40460">
        <property type="protein sequence ID" value="AAK40460"/>
        <property type="gene ID" value="SSO0104"/>
    </dbReference>
    <dbReference type="GeneID" id="1453288"/>
    <dbReference type="KEGG" id="sso:SSO0104"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.101"/>
    <dbReference type="eggNOG" id="arCOG00492">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_6_2"/>
    <dbReference type="InParanoid" id="P95957"/>
    <dbReference type="PhylomeDB" id="P95957"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000053">
        <property type="entry name" value="Aminotransferase, class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF19">
        <property type="entry name" value="KYNURENINE_ALPHA-AMINOADIPATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000123932" description="Uncharacterized aminotransferase SSO0104">
        <location>
            <begin position="1"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="401" mass="45572" checksum="BA5F6D8232FF59B4" modified="1997-05-01" version="1">MFERFLSTETKYLRTSEIRDLLKLTEGKNVISLAGGLPDPQTFPVEEIKKIADDILLNSADKALQYTATAGISEFRRELVNLSRLRGISGIDERNVFVTVGSQEALFMIFNILLDPGDNVVVEAPTYLAALNAMRTRKPNFISITVTEMGPDLDELERKIKDAHSNGKKVKLMYVIPTAQNPAGTTMNTEDRKRLLEIASKYDFLIFEDDAYGFLVFEGESPPPIKAFDKEGRVIYTSTFSKILAPGLRLGWVIAHEDFIKEMELYKQNVDLHTPSLSQYIAMEAIRRGIIQNNLPKIRRVYKEKRDVMLEAIETYFPNDARWTKPVGGMFVFAWLPQKIDTTKMLEKALQRGVAYVPGSSFYADYSGKNTMRINFSFPKKEELIEGIKRLGDTIKHELST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX56</accession>
    <name>Q7LX56_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF973 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74059">
        <property type="entry name" value="S74059"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923789.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX56"/>
    <dbReference type="STRING" id="273057.SSO2146"/>
    <dbReference type="PaxDb" id="273057-SSO2146"/>
    <dbReference type="EnsemblBacteria" id="AAK42324">
        <property type="protein sequence ID" value="AAK42324"/>
        <property type="gene ID" value="SSO2146"/>
    </dbReference>
    <dbReference type="GeneID" id="1453651"/>
    <dbReference type="KEGG" id="sso:SSO2146"/>
    <dbReference type="eggNOG" id="arCOG03871">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1782570_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LX56"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="14257" checksum="21A5F42F4837D7A2" modified="2011-05-31" version="1">MDMASRRKNARGLSGAVTALILVIASVIIALVVVGFAFGLFGAFTGQGTVTQVGTATLSASTLTLTVTLKNTGASTQVTGVLINGNSGSVSGMTTISAGVNTYTITISIGSISTTLRGLVGSTISLTLILSNGQTVTVSAIVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-06-18" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LX59</accession>
    <name>Q7LX59_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Second ORF in transposon ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2143</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S74055">
        <property type="entry name" value="S74055"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LX59"/>
    <dbReference type="STRING" id="273057.SSO2143"/>
    <dbReference type="PaxDb" id="273057-SSO2143"/>
    <dbReference type="EnsemblBacteria" id="AAK42321">
        <property type="protein sequence ID" value="AAK42321"/>
        <property type="gene ID" value="SSO2143"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2143"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2237"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_9_1_2"/>
    <dbReference type="InParanoid" id="Q7LX59"/>
    <dbReference type="PhylomeDB" id="Q7LX59"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="17"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="142"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="213" mass="24791" checksum="78C2948155EED79A" modified="2011-05-31" version="1">MVVKLTRSERIYITFVVDHEFPKLPNTGKEVAIDVGIEKLIVTSDGEYFPNLRPLEKALWKVKHLHRELSRKKFLSNNWFKAKVKLARAYEHLKNLRRDLYMKLGKWFAEHYDVAVMEGIHVKQLIGKSLRSLRRRLSDVAFSELRDLIKYQLEKYGKKLILVNPAYTSKTCAKCGYVKEDLSLSDRVFVCPNCGWIADRDYNASLNILRGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXD0</accession>
    <name>Q7LXD0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Microtubule binding protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1256</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AAK41495.1"/>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="AAK41495">
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        <property type="gene ID" value="SSO1256"/>
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    <dbReference type="GeneID" id="71027426"/>
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            <begin position="47"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="464" mass="53701" checksum="A06FA539D20118C3" modified="2011-05-31" version="1">MLKDDILKLLKEDAEFRKQVEEILGISFINVTLADLKDILKGLLASMDKLKSSVDQLVDAQRRAEERIAKLENAVEQLVEAQKRTDERITKLEESTKKLEQAVQELIEAQKKHDERITKLEESTKKLEQAVQELIEAQKKHDERITKLEESTKKLEQAVQELIEAQKKHDERITKLEESTKKLEQAVQELIEAQKKHDERITKLEESTKKLEQAVQELIEAQKKHDERITKLEESTKKLEQAVQELIEAQKKHDERITKLEESIQKLVDAQRRAEERIAKLENAVEQLVEAQKRTDERITKLEEVTMKLVESQLGMQNEIRELRKALGSMGKRWGRDFEKLIIEIVDELAKQEGLDLKYVNKFTYKDDNGLFGLKGVEYDVDLLIKDTKVYLIEIKSYVEKDDVNWAAHKFNVIEKALGLKNTIKMIFAVDATNLATKKGEELGIKVVYGSQSEEEEKGEVKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-04-02" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXF0</accession>
    <name>Q7LXF0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0828</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D99233">
        <property type="entry name" value="D99233"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923118.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXF0"/>
    <dbReference type="PaxDb" id="273057-SSO0828"/>
    <dbReference type="EnsemblBacteria" id="AAK41123">
        <property type="protein sequence ID" value="AAK41123"/>
        <property type="gene ID" value="SSO0828"/>
    </dbReference>
    <dbReference type="GeneID" id="1455090"/>
    <dbReference type="KEGG" id="sso:SSO0828"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.842"/>
    <dbReference type="eggNOG" id="arCOG07726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2285180_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXF0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="101" mass="11881" checksum="2BD427F7100CAABD" modified="2011-05-31" version="1">MSVENISFFGEFLIISPYYILRNNGILFDETYINGVEDFDLFLNILSTGSYKSIRFNIEHLGGRSLGTDDKRFLRNYINMIYLNYKIVNKVIKIKKINIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXF3</accession>
    <name>Q7LXF3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YfhO family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99233">
        <property type="entry name" value="A99233"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXF3"/>
    <dbReference type="STRING" id="273057.SSO0825"/>
    <dbReference type="PaxDb" id="273057-SSO0825"/>
    <dbReference type="EnsemblBacteria" id="AAK41120">
        <property type="protein sequence ID" value="AAK41120"/>
        <property type="gene ID" value="SSO0825"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0825"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.836"/>
    <dbReference type="eggNOG" id="arCOG06018">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_367873_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXF3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="325"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="370"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="405"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="730"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="760" mass="87507" checksum="92DCA1D987078A29" modified="2011-05-31" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXF9</accession>
    <name>Q7LXF9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TRM5/TYW2-like methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO0818</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90232">
        <property type="entry name" value="C90232"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923109.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXF9"/>
    <dbReference type="SMR" id="Q7LXF9"/>
    <dbReference type="STRING" id="273057.SSO0818"/>
    <dbReference type="PaxDb" id="273057-SSO0818"/>
    <dbReference type="EnsemblBacteria" id="AAK41114">
        <property type="protein sequence ID" value="AAK41114"/>
        <property type="gene ID" value="SSO0818"/>
    </dbReference>
    <dbReference type="GeneID" id="1455081"/>
    <dbReference type="KEGG" id="sso:SSO0818"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.830"/>
    <dbReference type="eggNOG" id="arCOG01400">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080078_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXF9"/>
    <dbReference type="PhylomeDB" id="Q7LXF9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRM5/TYW2-like methyltransferase" evidence="3">
        <location>
            <begin position="102"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="244" mass="28127" checksum="CA1197451AF5A006" modified="2011-05-31" version="1">MEFIPPKRLLELIRQYSTTFENWHSVMLSIYLHKERIKCKLRNGQLLYLKPNEIVLLAGLKIVELNEDSVRFQFNGREICLKGWKLSYPGDSFSDYWRIDVRGKRVLDIGAGIGDSPIYFSIAGAKEVVAVEVDSKKVELMRENLKANSINNVIVVDKGVGIKDDENYISWPKLISNYGPFDVAKIDCDGCEKIIVPYIKEIPSLLIEWDYNYKDIVSNLTKNGYKVKVEHTLKNLGFIYAKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXL4</accession>
    <name>Q7LXL4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosome assembly factor SBDS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO0737</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SDO1/SBDS family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90222">
        <property type="entry name" value="B90222"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991311.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXL4"/>
    <dbReference type="FunCoup" id="Q7LXL4">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0737"/>
    <dbReference type="PaxDb" id="273057-SSO0737"/>
    <dbReference type="EnsemblBacteria" id="AAK41033">
        <property type="protein sequence ID" value="AAK41033"/>
        <property type="gene ID" value="SSO0737"/>
    </dbReference>
    <dbReference type="GeneID" id="1455000"/>
    <dbReference type="KEGG" id="sso:SSO0737"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.733"/>
    <dbReference type="eggNOG" id="arCOG04187">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043216_2_0_2"/>
    <dbReference type="InParanoid" id="Q7LXL4"/>
    <dbReference type="PhylomeDB" id="Q7LXL4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.900">
        <property type="entry name" value="SBDS protein C-terminal domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002140">
        <property type="entry name" value="Sdo1/SBDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046928">
        <property type="entry name" value="SDO1/SBDS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018978">
        <property type="entry name" value="SDO1/SBDS_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037188">
        <property type="entry name" value="Sdo1/SBDS_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00291">
        <property type="entry name" value="RNA_SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927:SF4">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SDO1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20268">
        <property type="entry name" value="SBDS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09377">
        <property type="entry name" value="SBDS_domain_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109728">
        <property type="entry name" value="Hypothetical protein AF0491, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS central" evidence="3">
        <location>
            <begin position="101"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS C-terminal" evidence="4">
        <location>
            <begin position="165"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="26200" checksum="4E4A012EC3A0BFCE" modified="2011-05-31" version="1">MTKERDYVIVKYESHGERFEILAKPKEALAFRSGKSISLSDVVVSDTIYKDVKKGLKASPASLKKVFGTTDFETIVKEILLKGELPVTAEQRKEMLETKRKQIIDFIHRNAVDPKTNLPIPPTRLEMAMEQARIQIDLNKDVEAQAMQIVKEISKIIPIKIARALLSIKVPSEYSSKVKSQLHNLGEVKKANWLEDGTLLAELEIPAGAQQDVIDKLNSLTKGEVEVKVLQVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXV0</accession>
    <name>Q7LXV0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mechanosensitive ion channel MscS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0550</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90201">
        <property type="entry name" value="D90201"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923030.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXV0"/>
    <dbReference type="STRING" id="273057.SSO0550"/>
    <dbReference type="PaxDb" id="273057-SSO0550"/>
    <dbReference type="EnsemblBacteria" id="AAK40867">
        <property type="protein sequence ID" value="AAK40867"/>
        <property type="gene ID" value="SSO0550"/>
    </dbReference>
    <dbReference type="GeneID" id="1454834"/>
    <dbReference type="KEGG" id="sso:SSO0550"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.559"/>
    <dbReference type="eggNOG" id="arCOG01568">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074283_0_0_2"/>
    <dbReference type="InParanoid" id="Q7LXV0"/>
    <dbReference type="PhylomeDB" id="Q7LXV0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221:SF1">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="2">
        <location>
            <begin position="126"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="314" mass="35725" checksum="C8A2B40354992EE8" modified="2011-05-31" version="1">MRMSLRIAILLFLLFLAFSLGFVIQYFLQVFGITNQSTINSVIFYLRIALYVFIGLWIVSSISEAIRVYSQRRLGIRANVIANSVRYFGYVIVFLLVLLPLGVGSSTLIAGSTFAGLIIGLALQPVLSNFFAGLLIMLTGYIVVGDKIRVLSTQVPFFPAQFPAYKYFSTDFIEQGYKGTVVEIDLFYSRVLLENLRELRVPNIVLLNSAVLDYTSKYSEEHVINVRVEFPLAFIDINKLEDIVKEELKDFNVVEGPYINEQSDKDHVIVLARLKVGVSEDWRKIKSNALKRLLRLRQELIDKKSQDSQQTKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXV2</accession>
    <name>Q7LXV2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug resistance related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO0548</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90201">
        <property type="entry name" value="B90201"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="AlphaFoldDB" id="Q7LXV2"/>
    <dbReference type="FunCoup" id="Q7LXV2">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO0548"/>
    <dbReference type="DNASU" id="1454832"/>
    <dbReference type="EnsemblBacteria" id="AAK40865">
        <property type="protein sequence ID" value="AAK40865"/>
        <property type="gene ID" value="SSO0548"/>
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    <dbReference type="GeneID" id="54123226"/>
    <dbReference type="KEGG" id="sso:SSO0548"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.553"/>
    <dbReference type="eggNOG" id="arCOG00132">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_701358_0_0_2"/>
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        <property type="component" value="Chromosome"/>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
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        <property type="entry name" value="MFS"/>
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        <property type="entry name" value="MFS_dom"/>
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        <property type="entry name" value="MFS_trans_sf"/>
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    <dbReference type="InterPro" id="IPR050171">
        <property type="entry name" value="MFS_Transporters"/>
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    <dbReference type="PANTHER" id="PTHR23517:SF3">
        <property type="entry name" value="INTEGRAL MEMBRANE TRANSPORT PROTEIN"/>
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    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
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    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="20"/>
            <end position="40"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="75"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
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        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
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        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
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        <location>
            <begin position="243"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="367"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="18"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK40865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="393" mass="44180" checksum="66968D60357670FE" modified="2011-05-31" version="1">MGLKQTLKEIYYIVADKRLLYFSLSLGISTFAYSLLTYYFPIIMTNLDITTFTIGIIYSIISFLYVVFNIPLGAIVDKIGSKNAVTLSALMAVPLFLLMGTLNPLLFIISLVIFESVIRIVNSLGAHRFMLNYTNAGKAFGVFSLITSILAAIGIMLGGYLLQHSVSTLLFTIIAVLFGISGIIRFLELPKDENKVEVKRSLRLGFKYLKEDKKLLLYLLVSILSSGLSLETYYVTIYFVKDLLLPLTFVGVLYSSYALIMAFLPLLFSVILSKRSNFKNLSILIFSNSVIFFLVPLFTNVYILFLLFYAWTVIVAMESIVDYNVAQSVTKPEIRGTQVALISTFVRIFSVFYNAIVGLLFQITPLYSFYFTGFLGILAIFTIKFAEFIGVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U33</accession>
    <name>Q97U33_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO3189</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="B90504"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
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    <dbReference type="STRING" id="273057.SSO3189"/>
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    <dbReference type="EnsemblBacteria" id="AAK43289">
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        <property type="gene ID" value="SSO3189"/>
    </dbReference>
    <dbReference type="GeneID" id="1453208"/>
    <dbReference type="KEGG" id="sso:SSO3189"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3294"/>
    <dbReference type="eggNOG" id="arCOG03465">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034270_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U33"/>
    <dbReference type="PhylomeDB" id="Q97U33"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF7">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="335"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="408"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="515" mass="56185" checksum="ED7BDF35AB065B64" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2014-10-29" modified="2025-06-18" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UA0</accession>
    <name>KDAD_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>2-dehydro-3-deoxy-D-arabinonate dehydratase</fullName>
            <ecNumber evidence="6">4.2.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">2-keto-3-deoxy-D-arabinonate dehydratase</fullName>
            <shortName evidence="3">KdaD</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">kdaD</name>
        <name evidence="8" type="ordered locus">SSO3118</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="J. Biol. Chem." volume="281" first="27378" last="27388">
            <title>Identification of the missing links in prokaryotic pentose oxidation pathways: evidence for enzyme recruitment.</title>
            <authorList>
                <person name="Brouns S.J."/>
                <person name="Walther J."/>
                <person name="Snijders A.P."/>
                <person name="van de Werken H.J."/>
                <person name="Willemen H.L."/>
                <person name="Worm P."/>
                <person name="de Vos M.G."/>
                <person name="Andersson A."/>
                <person name="Lundgren M."/>
                <person name="Mazon H.F."/>
                <person name="van den Heuvel R.H."/>
                <person name="Nilsson P."/>
                <person name="Salmon L."/>
                <person name="de Vos W.M."/>
                <person name="Wright P.C."/>
                <person name="Bernander R."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="16849334"/>
            <dbReference type="DOI" id="10.1074/jbc.M605549200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>INDUCTION BY D-ARABINOSE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="J. Mol. Biol." volume="379" first="357" last="371">
            <title>Structural insight into substrate binding and catalysis of a novel 2-keto-3-deoxy-D-arabinonate dehydratase illustrates common mechanistic features of the FAH superfamily.</title>
            <authorList>
                <person name="Brouns S.J."/>
                <person name="Barends T.R."/>
                <person name="Worm P."/>
                <person name="Akerboom J."/>
                <person name="Turnbull A.P."/>
                <person name="Salmon L."/>
                <person name="van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="18448118"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2008.03.064"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 6-298 IN COMPLEX WITH THE SUBSTRATE ANALOG 2-OXOBUTANOATE; THE PRODUCT 2,5-DIOXOPENTANOATE; CALCIUM AND MAGNESIUM</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Participates in a pentose oxidation pathway that converts D-arabinonate to 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-dehydro-3-deoxy-D-arabinonate = 2,5-dioxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:35807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16699"/>
            <dbReference type="ChEBI" id="CHEBI:58136"/>
            <dbReference type="EC" id="4.2.1.141"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
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        <text evidence="1 2">Homotetramer.</text>
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        <text evidence="1">Expression is strongly increased by growth on D-arabinose, both at the mRNA and at the protein level.</text>
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        <text evidence="5">Belongs to the FAH family.</text>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UE7</accession>
    <name>Q97UE7_SACS2</name>
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        <submittedName>
            <fullName evidence="2">Partial transposase in ISC1190</fullName>
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        <name evidence="2" type="ordered locus">SSO3075</name>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UF8</accession>
    <name>Q97UF8_SACS2</name>
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    </organism>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90488">
        <property type="entry name" value="D90488"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UF8"/>
    <dbReference type="PaxDb" id="273057-SSO3062"/>
    <dbReference type="EnsemblBacteria" id="AAK43163">
        <property type="protein sequence ID" value="AAK43163"/>
        <property type="gene ID" value="SSO3062"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO3062"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3162"/>
    <dbReference type="eggNOG" id="arCOG04558">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2010197_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UF8"/>
    <dbReference type="PhylomeDB" id="Q97UF8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052775">
        <property type="entry name" value="IUN_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190:SF1">
        <property type="entry name" value="SI:CH211-201H21.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190">
        <property type="entry name" value="SI:CH211-201H21.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="123" mass="14034" checksum="7EF1BB3EDDE23100" modified="2001-10-01" version="1">MGFRVSKYRYSSISNSKRPLVKSFKTVEDVHGKGGVGNEIVKPIRLKAQSKHAFDAITELCETYFKVLEFLAISPLTNLALAYLKYPRLTECIHHLYIMGGTIYGRGNITPIAEYNFWVDQMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UG0</accession>
    <name>Q97UG0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH bat-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90488">
        <property type="entry name" value="B90488"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988416.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UG0"/>
    <dbReference type="STRING" id="273057.SSO3061"/>
    <dbReference type="PaxDb" id="273057-SSO3061"/>
    <dbReference type="EnsemblBacteria" id="AAK43161">
        <property type="protein sequence ID" value="AAK43161"/>
        <property type="gene ID" value="SSO3061"/>
    </dbReference>
    <dbReference type="GeneID" id="1453080"/>
    <dbReference type="KEGG" id="sso:SSO3061"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3159"/>
    <dbReference type="eggNOG" id="arCOG02272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104910_1_0_2"/>
    <dbReference type="InParanoid" id="Q97UG0"/>
    <dbReference type="PhylomeDB" id="Q97UG0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH bat-type" evidence="1">
        <location>
            <begin position="190"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="245" mass="28439" checksum="6109D983FA2CF3D1" modified="2001-10-01" version="1">MLSLTYNWKAFKLLKKYELVSHKYINVMVSHHGCWSELTSVNYIDTLINIEYSSFEILKASRIAMIRGSKENIKGQIKNIITKDRSIIDFDISEIVSDNNKYSIYLISMLQEAENTMISKLRAKGTSIIGTTIRDGKEIYDVIVPRDVKTLVVDAIMKDDRVKIINFVEKDVNEELIFKVISRNSFEMLLTEKERMLLRKARELGYFNVPRDRNSQELAKQLGVSKMNISFSLRKILKKISDLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UI3</accession>
    <name>Q97UI3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Xylose isomerase-like TIM barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO3034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90485">
        <property type="entry name" value="A90485"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924102.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UI3"/>
    <dbReference type="STRING" id="273057.SSO3034"/>
    <dbReference type="PaxDb" id="273057-SSO3034"/>
    <dbReference type="EnsemblBacteria" id="AAK43136">
        <property type="protein sequence ID" value="AAK43136"/>
        <property type="gene ID" value="SSO3034"/>
    </dbReference>
    <dbReference type="GeneID" id="1453055"/>
    <dbReference type="KEGG" id="sso:SSO3034"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3133"/>
    <dbReference type="eggNOG" id="arCOG01896">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1418748_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UI3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050312">
        <property type="entry name" value="IolE/XylAMocC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110">
        <property type="entry name" value="HYDROXYPYRUVATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110:SF21">
        <property type="entry name" value="XYLOSE ISOMERASE-LIKE TIM BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="1">
        <location>
            <begin position="20"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="191" mass="22021" checksum="234CBD4B2B43246E" modified="2001-10-01" version="1">MEIVFTSLAYPELSLEDVVERVSRFGFDGLELRVADDGKHLKPEFPINRKALEILSSIKISDLAGYTRFHFSDESERKRNEKALETLIKMANSLNALGVRVYGGEFSEKSAIHRIAESLNRMNRIAEDYSVKILIETHDSLAKKDNIVKLLQELEEDIGFVYDPANVIYRQWCFTKLFHLKDIILVLSKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UL3</accession>
    <name>Q97UL3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2991</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99480">
        <property type="entry name" value="A99480"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992644.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UL3"/>
    <dbReference type="STRING" id="273057.SSO2991"/>
    <dbReference type="PaxDb" id="273057-SSO2991"/>
    <dbReference type="EnsemblBacteria" id="AAK43096">
        <property type="protein sequence ID" value="AAK43096"/>
        <property type="gene ID" value="SSO2991"/>
    </dbReference>
    <dbReference type="GeneID" id="1453015"/>
    <dbReference type="KEGG" id="sso:SSO2991"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3085"/>
    <dbReference type="eggNOG" id="arCOG08504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1425148_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UL3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="187" mass="20784" checksum="6821EACEA1649AA5" modified="2001-10-01" version="1">MSLNILFLEFILASSFLLIISTVLQFYLESKLPGLTKDLDKVVFLAKLGALLSLIKLLSSDKISDILEGTMIAGPLNIKIEELKNYISANWDSLKGYISILNEKIKDVDRIIFLSKEVSVTMSHIVNENKISLVLLFCSSLFLLLNFVGIAFLFSGLAFGILAIAIASSLNCVKYVDELKDFFSKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UM2</accession>
    <name>Q97UM2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO11614</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90478">
        <property type="entry name" value="G90478"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992581.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UM2"/>
    <dbReference type="STRING" id="273057.SSO11614"/>
    <dbReference type="PaxDb" id="273057-SSO11614"/>
    <dbReference type="EnsemblBacteria" id="AAK43086">
        <property type="protein sequence ID" value="AAK43086"/>
        <property type="gene ID" value="SSO11614"/>
    </dbReference>
    <dbReference type="GeneID" id="1453005"/>
    <dbReference type="KEGG" id="sso:SSO11614"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3073"/>
    <dbReference type="eggNOG" id="arCOG07259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2713017_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UM2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="70" mass="8403" checksum="7CDFFBEBBCAD9CD6" modified="2001-10-01" version="1">MNYSKFWARFKEWALTTNDEVILPHKLRKIVEIIKRNPDITLVRLAGYLDTDALYLARYLRNSYKNIVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UM3</accession>
    <name>Q97UM3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Epimerase, putative</fullName>
            <ecNumber evidence="2">5.1.3.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.3.2" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90478">
        <property type="entry name" value="F90478"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UM3"/>
    <dbReference type="STRING" id="273057.SSO2981"/>
    <dbReference type="PaxDb" id="273057-SSO2981"/>
    <dbReference type="EnsemblBacteria" id="AAK43085">
        <property type="protein sequence ID" value="AAK43085"/>
        <property type="gene ID" value="SSO2981"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2981"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3072"/>
    <dbReference type="eggNOG" id="arCOG03016">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_917087_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UM3"/>
    <dbReference type="PhylomeDB" id="Q97UM3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF13">
        <property type="entry name" value="UDP-D-APIOSE_UDP-D-XYLOSE SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="303" mass="35165" checksum="A73A4B53522E01DF" modified="2001-10-01" version="1">MKILTFGGTGFVGSNFVRYAISKGHDVLVYARSMNDYTKALQNIGANIVFSYDNYLKDIDCLVYFIGAMWTKDPREFEYLQVQFPTEIGRKFFQVNSGKFVYISSIGVSENIESKERPIAEESPHGEGLKPNTLHGITKLEGERNISKFPNYVILRFPIVYGPYSRILMWRAFMWLVSHGIGLKNDNMFSVVSTRNTSKAIELACKYKRSDYFYITDKEPANLTSLFSDAVKAINREIRSWLPFSVKLIEPFKSSHEMLKMAYDVLSRELVYSYKKAERELNYIPEDVRIETFKEMAKYYKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UR6</accession>
    <name>Q97UR6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-methylhydantoinase A (HyuA-3)</fullName>
            <ecNumber evidence="4">3.5.2.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hyuA-3</name>
        <name evidence="4" type="ordered locus">SSO2936</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.14" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90473">
        <property type="entry name" value="A90473"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UR6"/>
    <dbReference type="FunCoup" id="Q97UR6">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2936"/>
    <dbReference type="PaxDb" id="273057-SSO2936"/>
    <dbReference type="EnsemblBacteria" id="AAK43040">
        <property type="protein sequence ID" value="AAK43040"/>
        <property type="gene ID" value="SSO2936"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2936"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3024"/>
    <dbReference type="eggNOG" id="arCOG01511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002157_1_2_2"/>
    <dbReference type="InParanoid" id="Q97UR6"/>
    <dbReference type="PhylomeDB" id="Q97UR6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047423">
        <property type="term" value="F:N-methylhydantoinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049517">
        <property type="entry name" value="ACX-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF23">
        <property type="entry name" value="HYPOTHETICAL 5-OXOPROLINASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19278">
        <property type="entry name" value="Hydant_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="1">
        <location>
            <begin position="199"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Acetophenone carboxylase-like C-terminal" evidence="3">
        <location>
            <begin position="563"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK43040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="650" mass="71886" checksum="50D542B533EE9920" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UT8</accession>
    <name>Q97UT8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phytoene synthetase (CrtB)</fullName>
            <ecNumber evidence="2">2.5.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">crtB</name>
        <name evidence="2" type="ordered locus">SSO2905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.-" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90469">
        <property type="entry name" value="G90469"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009993322.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UT8"/>
    <dbReference type="FunCoup" id="Q97UT8">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2905"/>
    <dbReference type="PaxDb" id="273057-SSO2905"/>
    <dbReference type="EnsemblBacteria" id="AAK43014">
        <property type="protein sequence ID" value="AAK43014"/>
        <property type="gene ID" value="SSO2905"/>
    </dbReference>
    <dbReference type="GeneID" id="1452933"/>
    <dbReference type="KEGG" id="sso:SSO2905"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2995"/>
    <dbReference type="eggNOG" id="arCOG02936">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037269_1_2_2"/>
    <dbReference type="InParanoid" id="Q97UT8"/>
    <dbReference type="PhylomeDB" id="Q97UT8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046905">
        <property type="term" value="F:15-cis-phytoene synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051996">
        <property type="term" value="F:squalene synthase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00683">
        <property type="entry name" value="Trans_IPPS_HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
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        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019845">
        <property type="entry name" value="Squalene/phytoene_synthase_CS"/>
    </dbReference>
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        <property type="entry name" value="Trans_IPPS_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033904">
        <property type="entry name" value="Trans_IPPS_HH"/>
    </dbReference>
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        <property type="entry name" value="BIFUNCTIONAL LYCOPENE CYCLASE/PHYTOENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01212">
        <property type="entry name" value="Phytoene_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01018">
        <property type="entry name" value="Squalene/Phytoene_Synthase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01045">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK43014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="277" mass="33131" checksum="092F13AF3C26C4A2" modified="2001-10-01" version="1">MIEKLEEIFKKGSVTYYNSSYFFPKKIREDVTKLYAFVRVFDDLVDSVPQKIEEYYNLRKLYQLEKSGYNTGNIVLQNFVELARRKGFEDSWIDAFLDAMESDIYKKVYYSIEELLKYMYGSAEVVGLFMMKILDLHNDSMYYARMLGRAMQFLNFIRDVQEDLYLGRQYLPVEDMEKFGIDKMECNEGFIEFIRYEISRYFEFEREAEKGFKFIPARYLVPIKTASEMYKWSALRIKKSPCIVLSKKVKPRRTKIVITVIKNFAGVYLWRSLNPII</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UW7</accession>
    <name>Q97UW7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Medium-chain-fatty-acid--CoA ligase (AlkK-4)</fullName>
            <ecNumber evidence="3">6.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">alkK-4</name>
        <name evidence="3" type="ordered locus">SSO2875</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.-" evidence="3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90465">
        <property type="entry name" value="F90465"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989824.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UW7"/>
    <dbReference type="STRING" id="273057.SSO2875"/>
    <dbReference type="PaxDb" id="273057-SSO2875"/>
    <dbReference type="EnsemblBacteria" id="AAK42981">
        <property type="protein sequence ID" value="AAK42981"/>
        <property type="gene ID" value="SSO2875"/>
    </dbReference>
    <dbReference type="GeneID" id="1452900"/>
    <dbReference type="KEGG" id="sso:SSO2875"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2960"/>
    <dbReference type="eggNOG" id="arCOG00856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_5_2"/>
    <dbReference type="InParanoid" id="Q97UW7"/>
    <dbReference type="PhylomeDB" id="Q97UW7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12119">
        <property type="entry name" value="ttLC_FACS_AlkK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050237">
        <property type="entry name" value="ATP-dep_AMP-bd_enzyme"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004837">
        <property type="entry name" value="PRK06187.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43767:SF11">
        <property type="entry name" value="MEDIUM-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="2">
        <location>
            <begin position="447"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="537" mass="60974" checksum="A2BD3F3E6E86F681" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UY3</accession>
    <name>Q97UY3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2856</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90463">
        <property type="entry name" value="F90463"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UY3"/>
    <dbReference type="STRING" id="273057.SSO2856"/>
    <dbReference type="PaxDb" id="273057-SSO2856"/>
    <dbReference type="EnsemblBacteria" id="AAK42965">
        <property type="protein sequence ID" value="AAK42965"/>
        <property type="gene ID" value="SSO2856"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2856"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2943"/>
    <dbReference type="eggNOG" id="arCOG00132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870837_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UY3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050171">
        <property type="entry name" value="MFS_Transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517:SF14">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517">
        <property type="entry name" value="RESISTANCE PROTEIN MDTM, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="136" mass="14995" checksum="0CECD6B614903E69" modified="2001-10-01" version="1">MIVAFQDLVGRLISKRMWLIVIGTLIYTSGYFGVAFISNFLVASIDIAIITIAEMIVTPLSQAIANSLTNQSSRGRQIGLYSMVTGIGRVSGSSLISELMNYYLYTPVILWGIMSSFGLVSAAIYLYQIKIKRIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UY6</accession>
    <name>Q97UY6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Permease, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2853</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90463">
        <property type="entry name" value="C90463"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UY6"/>
    <dbReference type="STRING" id="273057.SSO2853"/>
    <dbReference type="PaxDb" id="273057-SSO2853"/>
    <dbReference type="EnsemblBacteria" id="AAK42962">
        <property type="protein sequence ID" value="AAK42962"/>
        <property type="gene ID" value="SSO2853"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2853"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2938"/>
    <dbReference type="eggNOG" id="arCOG00132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1080183_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UY6"/>
    <dbReference type="PhylomeDB" id="Q97UY6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050171">
        <property type="entry name" value="MFS_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517:SF14">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517">
        <property type="entry name" value="RESISTANCE PROTEIN MDTM, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="257" mass="28639" checksum="CA6FD7109950B5E8" modified="2001-10-01" version="1">MEEINRIAIIGGVRSFSGSVIWPFIGFALYKVYGFSLDTVSIFYLIQALINVVASVSGGIVVDYLGRRNAMMLSMIVSSFALFTAYLVNLPIPIAGLILLQTFFNNIYNVSSTAIVGDIYKRADLVKAFSRQRVGINAGWALGPLIGGYIFTFYGFRTLLLVSSLIAIIPIFLVRLLPEFKGGGNILNFNVNKQFILFLISTFLTFVLVGQLGFGLLTYYNAQLHFTEFQVSILSVVFHQIISLERYCVSSFKRELI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2019-07-03" modified="2025-06-18" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UZ0</accession>
    <name>GLCT_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucose import system permease protein GlcT</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">glcT</name>
        <name evidence="7" type="ordered locus">SSO2848</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="J. Bacteriol." volume="181" first="4285" last="4291">
            <title>Glucose transport in the extremely thermoacidophilic Sulfolobus solfataricus involves a high-affinity membrane-integrated binding protein.</title>
            <authorList>
                <person name="Albers S.V."/>
                <person name="Elferink M.G."/>
                <person name="Charlebois R.L."/>
                <person name="Sensen C.W."/>
                <person name="Driessen A.J."/>
                <person name="Konings W.N."/>
            </authorList>
            <dbReference type="PubMed" id="10400586"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Mol. Microbiol." volume="39" first="1494" last="1503">
            <title>Sugar transport in Sulfolobus solfataricus is mediated by two families of binding protein-dependent ABC transporters.</title>
            <authorList>
                <person name="Elferink M.G."/>
                <person name="Albers S.V."/>
                <person name="Konings W.N."/>
                <person name="Driessen A.J."/>
            </authorList>
            <dbReference type="PubMed" id="11260467"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="5 6">Part of the ABC transporter complex GlcSTUV involved in glucose uptake. Responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The complex is composed of two ATP-binding proteins (GlcV), two transmembrane proteins (GlcT and GlcU) and a solute-binding protein (GlcS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90462">
        <property type="entry name" value="G90462"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923999.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UZ0"/>
    <dbReference type="SMR" id="Q97UZ0"/>
    <dbReference type="FunCoup" id="Q97UZ0">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2848"/>
    <dbReference type="TCDB" id="3.A.1.1.13">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO2848"/>
    <dbReference type="EnsemblBacteria" id="AAK42958">
        <property type="protein sequence ID" value="AAK42958"/>
        <property type="gene ID" value="SSO2848"/>
    </dbReference>
    <dbReference type="GeneID" id="1452877"/>
    <dbReference type="GeneID" id="27429114"/>
    <dbReference type="KEGG" id="sso:SSO2848"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2934"/>
    <dbReference type="eggNOG" id="arCOG00157">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UZ0"/>
    <dbReference type="PhylomeDB" id="Q97UZ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054947">
        <property type="entry name" value="GlcT_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052730">
        <property type="entry name" value="Sugar_ABC_transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040931">
        <property type="entry name" value="ABC_arch_GlcT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43759:SF1">
        <property type="entry name" value="GLUCOSE IMPORT SYSTEM PERMEASE PROTEIN GLCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43759">
        <property type="entry name" value="TREHALOSE TRANSPORT SYSTEM PERMEASE PROTEIN SUGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0762">Sugar transport</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000447613" description="Glucose import system permease protein GlcT">
        <location>
            <begin position="1"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="2">
        <location>
            <begin position="63"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="11260467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="10400586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6">
        <source>
            <dbReference type="PubMed" id="11260467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="286" mass="32286" checksum="2478218F158ECB59" modified="2001-10-01" version="1">MMKKGTIILVAPTAIFSAILLYLVIWNAVMSFMNWSLLNSKPTFVGLETYSIVIRTFQFTNSLLHSIELSVILVVIGNILGIFIAALLYFLNSNKARSTFLSIVIYPLSISMAVNGLIWLWLFNIHIGVDWLLVRIGLPQFPWLSSTSTMFPSLVLVSVWAYTGIAALFYLAGFMNIDKTIVEAARLDGTSAFKILYKILIPNSLNSFIIATALLFLFSFRIFDLPYVLSGGTTNIFLQTSELYMYYLFTVEYFSQATAVATMITLIATIIIIPYALTVIRRWIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UZ3</accession>
    <name>Q97UZ3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Last part of transposase in ISC1250</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO11281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90462">
        <property type="entry name" value="C90462"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UZ3"/>
    <dbReference type="STRING" id="273057.SSO11281"/>
    <dbReference type="PaxDb" id="273057-SSO11281"/>
    <dbReference type="EnsemblBacteria" id="AAK42954">
        <property type="protein sequence ID" value="AAK42954"/>
        <property type="gene ID" value="SSO11281"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11281"/>
    <dbReference type="eggNOG" id="arCOG04918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2353343_0_0_2"/>
    <dbReference type="InParanoid" id="Q97UZ3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="96" mass="11483" checksum="0CCC48EC58494476" modified="2001-10-01" version="1">MCTMLDFFTERFYTGSSQLPRNFGSESNNLIESFNSLLERRFGKFHSPWRMLQIARTIANNYNLLTYFLIIVIILHCTYSSRFIRNIHNNFFIELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V49</accession>
    <name>Q97V49_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Second ORF in transposon ISC1225</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO11138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99455">
        <property type="entry name" value="A99455"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V49"/>
    <dbReference type="STRING" id="273057.SSO11138"/>
    <dbReference type="PaxDb" id="273057-SSO11138"/>
    <dbReference type="EnsemblBacteria" id="AAK42896">
        <property type="protein sequence ID" value="AAK42896"/>
        <property type="gene ID" value="SSO11138"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO11138"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2868"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2821097_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V49"/>
    <dbReference type="PhylomeDB" id="Q97V49"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252">
        <property type="entry name" value="THIRD ORF IN TRANSPOSON ISC1160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33252:SF2">
        <property type="entry name" value="TRANSPOSASE IS4-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="66" mass="7505" checksum="B3AF0952AED49AD0" modified="2001-10-01" version="1">MKQISLETLKGVEEMDLSLDWTSWYGKLVEGLGSSEKGYSWNYATTTKYEVKILILAFIPQVNDKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="109" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V97</accession>
    <name>Q97V97_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Deacetylase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2737</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2013-09" db="PDB data bank">
            <title>Biochemical characterization of a novel thermostable dinuclear carboxypeptidase from the thermoacidophilic archaeum Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Bauvois C."/>
                <person name="Demarez M."/>
                <person name="Durisotti V."/>
                <person name="Roovers M."/>
                <person name="Dutoit R."/>
                <person name="Gigot D."/>
                <person name="Dupuy J."/>
                <person name="Wattiez R."/>
                <person name="Legrain C."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.34 ANGSTROMS) IN COMPLEX WITH ZN(2+)</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90449">
        <property type="entry name" value="A90449"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990278.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="4MMO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.34 A"/>
        <property type="chains" value="A/B=1-437"/>
    </dbReference>
    <dbReference type="PDBsum" id="4MMO"/>
    <dbReference type="AlphaFoldDB" id="Q97V97"/>
    <dbReference type="SMR" id="Q97V97"/>
    <dbReference type="FunCoup" id="Q97V97">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2737"/>
    <dbReference type="PaxDb" id="273057-SSO2737"/>
    <dbReference type="EnsemblBacteria" id="AAK42848">
        <property type="protein sequence ID" value="AAK42848"/>
        <property type="gene ID" value="SSO2737"/>
    </dbReference>
    <dbReference type="GeneID" id="1452767"/>
    <dbReference type="KEGG" id="sso:SSO2737"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2817"/>
    <dbReference type="eggNOG" id="arCOG01110">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029469_2_1_2"/>
    <dbReference type="InParanoid" id="Q97V97"/>
    <dbReference type="PhylomeDB" id="Q97V97"/>
    <dbReference type="EvolutionaryTrace" id="Q97V97"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05681">
        <property type="entry name" value="M20_dipept_Sso-CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005034">
        <property type="entry name" value="PRK06446.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF8">
        <property type="entry name" value="DI- AND TRIPEPTIDASE DUG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="7" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="4">
        <location>
            <begin position="180"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="208"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="4MMO"/>
        </source>
    </evidence>
    <sequence length="437" mass="48961" checksum="10FC39960190272B" modified="2001-10-01" version="1">MDEELYTLIEFLKKPSISATGEGIDETANYLKETVEKLLGVKANLEKTKGHPVVYAEINVNAKKTLLIYNHYDVQPVDPISEWKRAPFSATIENDRIYARGASDNKGTLMARLFAIKHLLDKNELNVNVKLLYEGEEEIGSVNLEDYIEKNTNKLKADSVIMEGAGLDPKGRPQIVLGVKGLLYVELVLDYGTKDLHSSNAPLVRNPCIDLAKIISTLVDMGGRVLIEGFYDDVRELTEEERELIKKYDIDVEELKKALGFKELKYNEKEKIAEALLTYPTCNVDGFECGYTGKGSKTIVPHRAFAKLDFRLVPNQDPYKVFELLKKHLQKAGFNGEILAHGFEYPVRTSVNSTVVKAMIESAKKVYGTEPQVIPNSAGTQPMGLFVYKLGIRDAVSAIGAGGYYSNAHAPNENIKIDDYYKAIKHTEEFLKLYPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VB5</accession>
    <name>Q97VB5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Aminotransferase</fullName>
            <ecNumber evidence="4">2.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO2720</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90446">
        <property type="entry name" value="G90446"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988652.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VB5"/>
    <dbReference type="FunCoup" id="Q97VB5">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2720"/>
    <dbReference type="PaxDb" id="273057-SSO2720"/>
    <dbReference type="EnsemblBacteria" id="AAK42830">
        <property type="protein sequence ID" value="AAK42830"/>
        <property type="gene ID" value="SSO2720"/>
    </dbReference>
    <dbReference type="GeneID" id="1452749"/>
    <dbReference type="KEGG" id="sso:SSO2720"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2801"/>
    <dbReference type="eggNOG" id="arCOG00916">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_4_0_2"/>
    <dbReference type="InParanoid" id="Q97VB5"/>
    <dbReference type="PhylomeDB" id="Q97VB5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43094">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43094:SF1">
        <property type="entry name" value="AMINOTRANSFERASE CLASS-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="444" mass="50629" checksum="9D124D51F81991BC" modified="2001-10-01" version="1">MGEIEDKKIIRDHTFKTWSKQKGWDPIIVSSAKGVYFYDVEGKKYLDFSSQFVNVNLGYGNERVINSIKEQLDRLQYINPSFGADIRVKATKALLKVMPRNISKFFYSTSGTEANEAAIKISRFYKKPRYKILARYRSYHGSTEGSISLTGDYRRWFVEPNTMNGVVRIPEPYCFRCPLKLKYPDCGIACATYVDYVIRQEKNVAAMIIEPITGTNGVIVPPKEYMPLIRKIAKENDVLFIADEVMTGWGRVGEWFAVNLWDIHPDILTTAKGASASYVPIGITGVSKEIGEFFEDEVFAHGHTFEAHPVSLSAIPAVIEEYERLNILSHVKVMGDYLGKRLQELKERHRSIGDVRGVGLFWAIELVKDKNNTPFGGYDDKYEGYTTFVDVLARRLLIEKNTYVYNGPSWFIISPPLIINKEEIDEGVDAIDDILKEADKEFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VG2</accession>
    <name>Q97VG2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-oxoacyl-(Acyl carrier protein) reductase (FabG-8)</fullName>
            <ecNumber evidence="2">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fabG-8</name>
        <name evidence="2" type="ordered locus">SSO2664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="2"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90440">
        <property type="entry name" value="G90440"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988581.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VG2"/>
    <dbReference type="STRING" id="273057.SSO2664"/>
    <dbReference type="PaxDb" id="273057-SSO2664"/>
    <dbReference type="EnsemblBacteria" id="AAK42782">
        <property type="protein sequence ID" value="AAK42782"/>
        <property type="gene ID" value="SSO2664"/>
    </dbReference>
    <dbReference type="GeneID" id="1452701"/>
    <dbReference type="KEGG" id="sso:SSO2664"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2746"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_2"/>
    <dbReference type="InParanoid" id="Q97VG2"/>
    <dbReference type="PhylomeDB" id="Q97VG2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF122">
        <property type="entry name" value="NAD(P)-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="252" mass="27822" checksum="B2F26BEAC58BE39A" modified="2001-10-01" version="1">MIKLDLSGKVCLITGGTSGIGLKTAELFSKLGANVYVIGRREANFLSKNIYFKKVDLTRREDVLSFIEWYEKNVGTIHVLINNASQNSRYSVLDIPIKEWDEMISLNLTAPFLLSRMAAKLMIKRGIKGKIINISAIQSKFPLERSFAYVTTKGGLISMGRSLAVDLGKYGIQVITVLPGPIYSKDDEPPASLDKRAATLLGRMGRMIEISHLLAFLASDLNSFITGTEIIIDGGRIISRKPDPEEITKGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VG3</accession>
    <name>Q97VG3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1404 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2663</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90440">
        <property type="entry name" value="F90440"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988577.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VG3"/>
    <dbReference type="PaxDb" id="273057-SSO2663"/>
    <dbReference type="EnsemblBacteria" id="AAK42781">
        <property type="protein sequence ID" value="AAK42781"/>
        <property type="gene ID" value="SSO2663"/>
    </dbReference>
    <dbReference type="GeneID" id="1452700"/>
    <dbReference type="KEGG" id="sso:SSO2663"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2745"/>
    <dbReference type="eggNOG" id="arCOG06032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109678_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VG3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009844">
        <property type="entry name" value="DUF1404"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07185">
        <property type="entry name" value="DUF1404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="21004" checksum="9A17910FCEDA014F" modified="2001-10-01" version="1">MILSLVKDEKITFRPLVLPIVLLAIAVNPFTEGLEFYSPAIYMISHYLVYFSGIFVGYKYFKGDIISLILGLIPPILWHLPYFFAIGAAFITYRAILEITLLTGGILVGSSIRYVKFYLKVTLFALWMLGDSALAIIFIVSSPLYSNIVYKFSPYSPSTLPIAGVAMFIAMNVFLGYVIARYIKGIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VI8</accession>
    <name>Q97VI8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90437">
        <property type="entry name" value="E90437"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VI8"/>
    <dbReference type="STRING" id="273057.SSO2635"/>
    <dbReference type="PaxDb" id="273057-SSO2635"/>
    <dbReference type="EnsemblBacteria" id="AAK42756">
        <property type="protein sequence ID" value="AAK42756"/>
        <property type="gene ID" value="SSO2635"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2635"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2719"/>
    <dbReference type="eggNOG" id="arCOG00643">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_574416_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VI8"/>
    <dbReference type="PhylomeDB" id="Q97VI8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017003">
        <property type="entry name" value="UCP032675"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038049">
        <property type="entry name" value="SelD_rel_HyperS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032675">
        <property type="entry name" value="UCP032675"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="465" mass="52456" checksum="DFBEA2CD030C9089" modified="2001-10-01" version="1">MMAIDKLIDKFRVNLDKYKKMGLNPLSLATGCAVKVDLIDTVYPAIRKIRDELVKRNIEILPREDADIFVSREKIYIKRVINGGEFDADRAVSLIQVNQETSGNPDKFAEFLLKVYTSIKTTRKLTIGKGHSIVTSNPKGEVAVLDLFRLEGGKERSYTVANNDTIQIVDPLDDPGSQMQVDVAISNSLNDLFTKGVFQDLRMIPVVDAPMDDLKEQLLKNAENYSREYSIELLSDVQPNSKTLMIGATVIGKSDHELPTYYNRVNENMEILVTRPVGELTPINVFMWMLTVPELIEDMEARGITIQRVEEAKRKALMYMRKPNNEVAKIIYDHLPPFGGSFDENSHIAMTTDVTGPGLFVIKEFAEKAQVDVELFDIPVIDPDIHEFATENFIIPNSTAGTNGAIVIFAHKRVIDEIFDELKRKSQEPYIIGKVTGKGNGTVIVPPTITKYIHRNNVLRQFKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VI9</accession>
    <name>Q97VI9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90437">
        <property type="entry name" value="D90437"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988539.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VI9"/>
    <dbReference type="STRING" id="273057.SSO2634"/>
    <dbReference type="PaxDb" id="273057-SSO2634"/>
    <dbReference type="EnsemblBacteria" id="AAK42755">
        <property type="protein sequence ID" value="AAK42755"/>
        <property type="gene ID" value="SSO2634"/>
    </dbReference>
    <dbReference type="GeneID" id="1452674"/>
    <dbReference type="KEGG" id="sso:SSO2634"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2718"/>
    <dbReference type="eggNOG" id="arCOG02021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1830677_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VI9"/>
    <dbReference type="PhylomeDB" id="Q97VI9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="28"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="140" mass="16309" checksum="558342E1701FDDE1" modified="2001-10-01" version="1">MMLITERRSPFYPNIQNVPPSVIRKLVKNKAITLIDVRQPWEYEDHHIPGSILLPLDYFKELFPLVLSKNNLVKNGVAIICEHANRSTWLVYTEPSLFKEVKVYNMLGGIELWMIMGYEIEKGMDDNGTLWYKLLTKNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VM0</accession>
    <name>Q97VM0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thermopsin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2602</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90433">
        <property type="entry name" value="E90433"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VM0"/>
    <dbReference type="PaxDb" id="273057-SSO2602"/>
    <dbReference type="EnsemblBacteria" id="AAK42724">
        <property type="protein sequence ID" value="AAK42724"/>
        <property type="gene ID" value="SSO2602"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2602"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2683"/>
    <dbReference type="eggNOG" id="arCOG03671">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_302416_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VM0"/>
    <dbReference type="PhylomeDB" id="Q97VM0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="963"/>
            <end position="983"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="990" mass="111430" checksum="DBE2517A7FBA596A" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="144" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VQ4</accession>
    <name>Q97VQ4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Dihydrolipoamide dehydrogenase (PdhD-4)</fullName>
            <ecNumber evidence="12">1.8.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pdhD-4</name>
        <name evidence="12" type="ordered locus">SSO2559</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="12"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99429">
        <property type="entry name" value="A99429"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992731.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VQ4"/>
    <dbReference type="FunCoup" id="Q97VQ4">
        <property type="interactions" value="249"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2559"/>
    <dbReference type="PaxDb" id="273057-SSO2559"/>
    <dbReference type="EnsemblBacteria" id="AAK42688">
        <property type="protein sequence ID" value="AAK42688"/>
        <property type="gene ID" value="SSO2559"/>
    </dbReference>
    <dbReference type="GeneID" id="1454015"/>
    <dbReference type="KEGG" id="sso:SSO2559"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2641"/>
    <dbReference type="eggNOG" id="arCOG01068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_3_2"/>
    <dbReference type="InParanoid" id="Q97VQ4"/>
    <dbReference type="PhylomeDB" id="Q97VQ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0274">FAD</keyword>
    <keyword evidence="3 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5 9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="11">
        <location>
            <begin position="1"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="10">
        <location>
            <begin position="308"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK42688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="409" mass="45197" checksum="1F5F4FC27FEA39FA" modified="2001-10-01" version="1">MKIAVIGSGPAGVSASLELAKNNKVVLVEKEERLGGTCVLYGCIPSKAMLHPIHLASELEKLGKNITFDLGELRKLGQDASYRLSKGVEYMLEDNGVEVIHGIASLRSGQLIVNNETIQVDYVVLATGTYREVVKGIIYSEDLPYLNKDFKSVIIVGGDVGGVEFGWMLRKLGKEVVLVDKQSSLLPYLDKEVSNAITNYFSKIGVRLYLNRSVKEMKEKEVILENGEKLTADVVYMTFGRKPSIQGFEEINHNPFVIVDEYLRTSLSNVFAAGDIIGTHTAHEAMYAGKIAATNIMGGKKVFNKQGIPKVVYTHPTIAYVGSMEGKCVKINLVELGRAITEKEIDGFLKLCIKDDKIVGAQAFMKDAEEVISLISFLIRYNIKVNEIKDYVAPHPSYIEALTELLNRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-11-03" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VT7</accession>
    <name>PHP_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Aryldialkylphosphatase</fullName>
            <ecNumber>3.1.8.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Paraoxonase</fullName>
            <shortName>SsoPox</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Phosphotriesterase-like lactonase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">php</name>
        <name type="ordered locus">SSO2522</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2005" name="Extremophiles" volume="9" first="297" last="305">
            <title>A thermostable phosphotriesterase from the archaeon Sulfolobus solfataricus: cloning, overexpression and properties.</title>
            <authorList>
                <person name="Merone L."/>
                <person name="Mandrich L."/>
                <person name="Rossi M."/>
                <person name="Manco G."/>
            </authorList>
            <dbReference type="PubMed" id="15909078"/>
            <dbReference type="DOI" id="10.1007/s00792-005-0445-4"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 1-10</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="J. Mol. Biol." volume="379" first="1017" last="1028">
            <title>Structural basis for natural lactonase and promiscuous phosphotriesterase activities.</title>
            <authorList>
                <person name="Elias M."/>
                <person name="Dupuy J."/>
                <person name="Merone L."/>
                <person name="Mandrich L."/>
                <person name="Porzio E."/>
                <person name="Moniot S."/>
                <person name="Rochu D."/>
                <person name="Lecomte C."/>
                <person name="Rossi M."/>
                <person name="Masson P."/>
                <person name="Manco G."/>
                <person name="Chabriere E."/>
            </authorList>
            <dbReference type="PubMed" id="18486146"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2008.04.022"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS)</scope>
        <scope>COFACTOR</scope>
        <scope>METAL-BINDING SITES</scope>
        <scope>SUBUNIT</scope>
        <scope>CARBOXYLATION AT LYS-137</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Has a low paraoxonase activity. Also active, but with a lower activity, against other organo-phosphorus insecticides such as Dursban, Coumaphos, pNP-butanoate or parathion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol.</text>
            <dbReference type="EC" id="3.1.8.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="3">Binds 1 Co(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="3">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Inactivated by EDTA and o-phenanthroline.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">0.06 mM for paraoxon (at pH 8.0)</KM>
            <KM evidence="2">0.205 mM for methyl-paraoxon (at pH 8.0)</KM>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 7.0-9.0.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Thermostable.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Phosphotriesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.8.1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY775568">
        <property type="protein sequence ID" value="AAW47234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90424">
        <property type="entry name" value="F90424"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988477.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2VC5">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A/B/C/D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="2VC7">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.05 A"/>
        <property type="chains" value="A/B/C/D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="3UF9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.68 A"/>
        <property type="chains" value="A/B/C/D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="4KER">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A/B/C/D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="4KES">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B/C/D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="4KET">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B/C/D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="4KEU">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B/C/D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="4KEV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.65 A"/>
        <property type="chains" value="A/B/C/D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="4KEZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.85 A"/>
        <property type="chains" value="A/B/C/D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="4KF1">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B/C/D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="5VRI">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.15 A"/>
        <property type="chains" value="A/B/C/D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="5VRK">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.40 A"/>
        <property type="chains" value="A/B=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="5VSA">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B/C/D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="5W3U">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B/C/D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="5W3W">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.95 A"/>
        <property type="chains" value="A/B/C/D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="5W3Z">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.55 A"/>
        <property type="chains" value="A/B/C/D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="8SF2">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.50 A"/>
        <property type="chains" value="D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="8SF9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="8SFA">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.32 A"/>
        <property type="chains" value="D/S/b/t=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="8SFB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.40 A"/>
        <property type="chains" value="D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="8SFC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.40 A"/>
        <property type="chains" value="D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="8SFD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.50 A"/>
        <property type="chains" value="D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="8SFK">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.40 A"/>
        <property type="chains" value="D=1-314"/>
    </dbReference>
    <dbReference type="PDB" id="8SFM">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.50 A"/>
        <property type="chains" value="D=1-314"/>
    </dbReference>
    <dbReference type="PDBsum" id="2VC5"/>
    <dbReference type="PDBsum" id="2VC7"/>
    <dbReference type="PDBsum" id="3UF9"/>
    <dbReference type="PDBsum" id="4KER"/>
    <dbReference type="PDBsum" id="4KES"/>
    <dbReference type="PDBsum" id="4KET"/>
    <dbReference type="PDBsum" id="4KEU"/>
    <dbReference type="PDBsum" id="4KEV"/>
    <dbReference type="PDBsum" id="4KEZ"/>
    <dbReference type="PDBsum" id="4KF1"/>
    <dbReference type="PDBsum" id="5VRI"/>
    <dbReference type="PDBsum" id="5VRK"/>
    <dbReference type="PDBsum" id="5VSA"/>
    <dbReference type="PDBsum" id="5W3U"/>
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<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VV2</accession>
    <name>Q97VV2_SACS2</name>
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        <name type="synonym">Sulfolobus solfataricus</name>
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            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
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        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
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                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
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                <person name="Van der Oost J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="408"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="489" mass="53544" checksum="283CBA711E603F87" modified="2001-10-01" version="1">MTFIRYFYELTDLRNFMSSKLDEYLARIDRLPTWGLSYALLWAIGFSYFITLYDAVGNIGAALPYIPFINASQASLIASLGLFGYIPGSLGLGYLADRIGRRTVLIITVLLTAIGSLGMALSINFPMLAIFRFIEGAGIGGDLNLAMVYVSEFAPSAKRGKYANWIYLAGWIAVGVGATIAALLVTGLPSVGWRLAFGIAAIMAVAALVFRIRAPETVRFLVKKGRINEAESIVRIMEETAMKRARVQSLPEPKIINYSYQTTNPFSILAKRVFAKRLIGLILFWFFIYFVQYTFSTLWDYYGKFIGYSGTMLNQFVLLTGFSALGDTLMAFLLLIIIERTDRRLITQIGSIGWLVGMIVASYFAVHFNLLGMALTLGLITNAIGGGMSYLAGYLISSESFPTAARSTGFAITDGLGHLGGAIGPLLLFPLVVATGPINAWAIEAFPVVIAAVILWFTVPRTVGVRLEEINEIHLTPQQPQGGIIKSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VV3</accession>
    <name>Q97VV3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">4-hydroxybutyryl-CoA dehydratase (AbfD-1)</fullName>
            <ecNumber evidence="6">4.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">abfD-1</name>
        <name evidence="6" type="ordered locus">SSO2503</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.-" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90422">
        <property type="entry name" value="F90422"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923905.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VV3"/>
    <dbReference type="FunCoup" id="Q97VV3">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2503"/>
    <dbReference type="PaxDb" id="273057-SSO2503"/>
    <dbReference type="EnsemblBacteria" id="AAK42637">
        <property type="protein sequence ID" value="AAK42637"/>
        <property type="gene ID" value="SSO2503"/>
    </dbReference>
    <dbReference type="GeneID" id="15296775"/>
    <dbReference type="KEGG" id="sso:SSO2503"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2582"/>
    <dbReference type="eggNOG" id="arCOG02143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023920_1_0_2"/>
    <dbReference type="InParanoid" id="Q97VV3"/>
    <dbReference type="PhylomeDB" id="Q97VV3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3140.10">
        <property type="entry name" value="4-hydroxybutyryl-coa dehydratase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004925">
        <property type="entry name" value="HpaB/PvcC/4-BUDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024719">
        <property type="entry name" value="HpaB/PvcC/4-BUDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024674">
        <property type="entry name" value="HpaB/PvcC/4-BUDH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36117">
        <property type="entry name" value="4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36117:SF3">
        <property type="entry name" value="4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03241">
        <property type="entry name" value="HpaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11794">
        <property type="entry name" value="HpaB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000331">
        <property type="entry name" value="HpaA_HpaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpaB/PvcC/4-BUDH N-terminal" evidence="5">
        <location>
            <begin position="4"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="HpaB/PvcC/4-BUDH C-terminal" evidence="4">
        <location>
            <begin position="268"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="463" mass="52338" checksum="A41A57252D10AA80" modified="2001-10-01" version="1">MRSKEDFLKSLKDGRNLYYRGKLVEDITTHQILKTAALHAAKLYEYADRVYEDNKMGKMSKFFKVPWTSQDLLDRHKLIYDLTMYCNGVFNISQAIGSDAIFALMITAKQVDRKYGTDYSKRVEKYFERVAKEDLTLATAQTDVKGDRSKRPSEQVDPDMYVRVVDVKSDGIVVRGAKAHTTQSAVSDEIIVIPTRVMRDSDKDYAVAFAVPANTKGLKMYIRPIDEIEGNSSSVLSRKDYELETLTVFNDVFVPWDRVFLFKEYDYAGTLAMLFATFHRFTALSYRSATMNLYLGASKVASQVNGIENEKHVRDDIVDIILYKEIMRSSAIAAAVYPVNMEGIAVPNPLFTNVGKLYSNMHFHDVVRDLIDIAGGIIATMPSQEDLESDEGKNIVKYLRGSVDGEERAKVLKLAKELGASTFTGYLLTGMIHAEGSMEASKIELFRSYNFKEAENLVKRVLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-30" modified="2025-06-18" version="129" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VW8</accession>
    <name>SYTC_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Threonine--tRNA ligase catalytic subunit</fullName>
            <ecNumber>6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Threonyl-tRNA synthetase catalytic subunit</fullName>
            <shortName evidence="5">ThrRS-cat</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">thrS-cat</name>
        <name type="ordered locus">SSO2486</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="Proc. Natl. Acad. Sci. U.S.A." volume="101" first="10260" last="10265">
            <title>A freestanding proofreading domain is required for protein synthesis quality control in Archaea.</title>
            <authorList>
                <person name="Korencic D."/>
                <person name="Ahel I."/>
                <person name="Schelert J."/>
                <person name="Sacher M."/>
                <person name="Ruan B."/>
                <person name="Stathopoulos C."/>
                <person name="Blum P."/>
                <person name="Ibba M."/>
                <person name="Soell D."/>
            </authorList>
            <dbReference type="PubMed" id="15240874"/>
            <dbReference type="DOI" id="10.1073/pnas.0403926101"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2005" name="J. Biol. Chem." volume="280" first="571" last="577">
            <title>A unique hydrophobic cluster near the active site contributes to differences in borrelidin inhibition among threonyl-tRNA synthetases.</title>
            <authorList>
                <person name="Ruan B."/>
                <person name="Bovee M.L."/>
                <person name="Sacher M."/>
                <person name="Stathopoulos C."/>
                <person name="Poralla K."/>
                <person name="Francklyn C.S."/>
                <person name="Soell D."/>
            </authorList>
            <dbReference type="PubMed" id="15507440"/>
            <dbReference type="DOI" id="10.1074/jbc.M411039200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr) (PubMed:15240874). Also activates L-serine and transfers it to tRNA(Thr); unlike most archaea the editing function is found in a freestanding protein (ACQ980D1) (PubMed:15240874). In vitro when both subunits are present, or if the 2 subunits are fused, L-seryl-tRNA(Thr) is no longer produced, the 2 subunits edit incorrectly charged L-seryl-tRNA(Thr) (PubMed:15240874). Has no activity on correctly acylated L-seryl-tRNA(Ser) or L-threonyl-tRNA(Thr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="4">Inhibited by 1 uM borrelidin (BN).</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">55 mM for L-serine activation</KM>
            <KM evidence="3">0.11 mM for L-threonine activation</KM>
            <KM evidence="4">100 uM for L-threonine activation</KM>
            <text evidence="3">kcat is 1.3 sec(-1) for L-serine, 1.9 for L-threonine at 60 degrees Celsius (PubMed:15240874).</text>
        </kinetics>
    </comment>
    <comment type="subunit">
        <text evidence="1 6">Homodimer (By similarity). Probably interacts with its editing subunit (AC Q980D1); a subunit fusion (in the order edit-catalytic) is fully functional.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90420">
        <property type="entry name" value="G90420"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923890.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VW8"/>
    <dbReference type="SMR" id="Q97VW8"/>
    <dbReference type="FunCoup" id="Q97VW8">
        <property type="interactions" value="279"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2486"/>
    <dbReference type="PaxDb" id="273057-SSO2486"/>
    <dbReference type="EnsemblBacteria" id="AAK42622">
        <property type="protein sequence ID" value="AAK42622"/>
        <property type="gene ID" value="SSO2486"/>
    </dbReference>
    <dbReference type="GeneID" id="7808241"/>
    <dbReference type="KEGG" id="sso:SSO2486"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2567"/>
    <dbReference type="eggNOG" id="arCOG00401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008554_0_1_2"/>
    <dbReference type="InParanoid" id="Q97VW8"/>
    <dbReference type="PhylomeDB" id="Q97VW8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000002">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000001">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF44">
        <property type="entry name" value="THREONINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000101113" description="Threonine--tRNA ligase catalytic subunit">
        <location>
            <begin position="1"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Catalytic" evidence="2">
        <location>
            <begin position="139"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9YDW0"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="15240874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="15507440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="15240874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6">
        <source>
            <dbReference type="PubMed" id="15240874"/>
        </source>
    </evidence>
    <sequence length="545" mass="63231" checksum="D9CEA476A1A192B1" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W10</accession>
    <name>Q97W10_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SIS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2437</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90415">
        <property type="entry name" value="E90415"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923854.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W10"/>
    <dbReference type="STRING" id="273057.SSO2437"/>
    <dbReference type="PaxDb" id="273057-SSO2437"/>
    <dbReference type="EnsemblBacteria" id="AAK42580">
        <property type="protein sequence ID" value="AAK42580"/>
        <property type="gene ID" value="SSO2437"/>
    </dbReference>
    <dbReference type="GeneID" id="1453907"/>
    <dbReference type="KEGG" id="sso:SSO2437"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2518"/>
    <dbReference type="eggNOG" id="arCOG00059">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077866_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W10"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="1">
        <location>
            <begin position="10"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="275" mass="31255" checksum="EF50385ADBF4BE3F" modified="2001-10-01" version="1">MYADLIEREITQDYKVNLDLKLDNAYIAGAGDSYAVALTIEGKTNGRFKALDPFEGLHYENLDRPLVIVSVSGRPKSNILLATKFKGKTRLYVITANEDSQLARLADYLILIPYKPIIPLPGTLSFLMSLSAVYSLAGEQADEIKESGRLLNLANNPFFVGHKENYGIAYYAMLKFAEIFGYATNSERFEQFCHSPIFMTANRQIVLFRSDNEREKELINSIDYTDTQATDCSGAFCNSIILIKSIVNKMRKEKWDKIYFLENKKILNISSKMIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W14</accession>
    <name>Q97W14_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Carbon monoxide dehydrogenase, small chain (CutC-1)</fullName>
            <ecNumber evidence="7">1.2.7.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cutC-1</name>
        <name evidence="7" type="ordered locus">SSO2433</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.4" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99415">
        <property type="entry name" value="A99415"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W14"/>
    <dbReference type="STRING" id="273057.SSO2433"/>
    <dbReference type="PaxDb" id="273057-SSO2433"/>
    <dbReference type="EnsemblBacteria" id="AAK42576">
        <property type="protein sequence ID" value="AAK42576"/>
        <property type="gene ID" value="SSO2433"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2433"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2512"/>
    <dbReference type="eggNOG" id="arCOG01925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052511_3_1_2"/>
    <dbReference type="InParanoid" id="Q97W14"/>
    <dbReference type="PhylomeDB" id="Q97W14"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:anaerobic carbon-monoxide dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.120:FF:000003">
        <property type="entry name" value="Carbon monoxide dehydrogenase, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000020">
        <property type="entry name" value="Xanthine dehydrogenase iron-sulfur subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.120">
        <property type="entry name" value="[2Fe-2S]-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002888">
        <property type="entry name" value="2Fe-2S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036884">
        <property type="entry name" value="2Fe-2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051452">
        <property type="entry name" value="Diverse_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053590">
        <property type="entry name" value="Glyceraldehyde_DH_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041020">
        <property type="entry name" value="glyceraldDH_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44379">
        <property type="entry name" value="OXIDOREDUCTASE WITH IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44379:SF5">
        <property type="entry name" value="OXIDOREDUCTASE WITH IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01799">
        <property type="entry name" value="Fer2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47741">
        <property type="entry name" value="CO dehydrogenase ISP C-domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="6">
        <location>
            <begin position="13"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="171" mass="19152" checksum="A9321AF83EF84A7B" modified="2001-10-01" version="1">MIYMKVFEKDQKVKVHLKVNGQDYEVETEPRRLLVHVLRELGFTGVHIGCDTSNCGACTVIMDGRSVKSCTILAVEADGAEITTIEGLAKDGKLHPIQEAFWEKHGLQCGYCTPGMIMEAYWLLKENPNPTEEEIREGISGNLCRCTGYQNIIEAVKLASEKLRMEIPKAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-07-24" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W57</accession>
    <name>Q97W57_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nmd3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90409">
        <property type="entry name" value="D90409"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W57"/>
    <dbReference type="SMR" id="Q97W57"/>
    <dbReference type="STRING" id="273057.SSO2383"/>
    <dbReference type="PaxDb" id="273057-SSO2383"/>
    <dbReference type="EnsemblBacteria" id="AAK42531">
        <property type="protein sequence ID" value="AAK42531"/>
        <property type="gene ID" value="SSO2383"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2383"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2465"/>
    <dbReference type="eggNOG" id="arCOG04149">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065087_1_1_2"/>
    <dbReference type="InParanoid" id="Q97W57"/>
    <dbReference type="PhylomeDB" id="Q97W57"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="1">
        <location>
            <begin position="9"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="240" mass="27387" checksum="F41B454072BE7AFA" modified="2001-10-01" version="1">MKFMSGKFCVLCGKQNVDLIDSLCVDCYVKSKKLIETPKRVTGKYCKICGAQWIRGKWIRTSATSLASVVEDIIARELGSKMRIDKNVDEFAFDIKSIWKDPSGNSFATIEFRGKVRGKSFSQEAVISLEMERELCDSCFRKKTRYYEAIVQLRSKGKIGVDDKKRAFFESFFSTSIVDNISDVIEGREGVDYYFMSKSVARKLVSMISSIVDVEVNESYQNEKMKNGKREAKLVISLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W89</accession>
    <name>Q97W89_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Geranylgeranyl hydrogenase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2348</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90405">
        <property type="entry name" value="D90405"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989508.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W89"/>
    <dbReference type="STRING" id="273057.SSO2348"/>
    <dbReference type="PaxDb" id="273057-SSO2348"/>
    <dbReference type="EnsemblBacteria" id="AAK42499">
        <property type="protein sequence ID" value="AAK42499"/>
        <property type="gene ID" value="SSO2348"/>
    </dbReference>
    <dbReference type="GeneID" id="1453826"/>
    <dbReference type="KEGG" id="sso:SSO2348"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2432"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066799_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W89"/>
    <dbReference type="PhylomeDB" id="Q97W89"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF20">
        <property type="entry name" value="HYDROGENASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="3"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="332" mass="37641" checksum="DD1E851D746D85DF" modified="2001-10-01" version="1">MTRIAIVGAGPAGLSLAYFLKGSRKVEATVYESMPEPGLKPCAWGLITGIENIIPIPNETIISEIRGFRIYLDNKLVFDIRTDNKLGYIIDKPLFLKRLSQEVNVEFNSKVVKKDDKYYINDKSLDHDKVIFATGHYSVSKSMSIPAIQYITDYEIDKEVVEFYFYSGFLGYAWVFPDREGSKIGIGGYAEVPELKERLKQILKGRIRTFHGARVTDYGVIEDRLDGNYTGEALGTVYAITGEGIRPSIISSKILADSILNRKDFKKEFKKSKLYWSLNWHAKIIKMTKENDPGTARLSKALLNNDPQLILKFAIGEFNRVDLIKIFGRSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W97</accession>
    <name>Q97W97_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transport protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90404">
        <property type="entry name" value="D90404"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923840.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W97"/>
    <dbReference type="PaxDb" id="273057-SSO2338"/>
    <dbReference type="DNASU" id="1453818"/>
    <dbReference type="EnsemblBacteria" id="AAK42491">
        <property type="protein sequence ID" value="AAK42491"/>
        <property type="gene ID" value="SSO2338"/>
    </dbReference>
    <dbReference type="GeneID" id="1453818"/>
    <dbReference type="KEGG" id="sso:SSO2338"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2424"/>
    <dbReference type="eggNOG" id="arCOG05344">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_800770_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W97"/>
    <dbReference type="PhylomeDB" id="Q97W97"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="302"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="329" mass="36520" checksum="5EF578F785B288B0" modified="2001-10-01" version="1">MVGNKVKSFMLPIISYAVPTFVLVYPSFFVSWLSESMHLAYWEVFAIVALPFLGRIIGSFIYQVFKNSVISYCFPFLGFLVILQNFLGALIFVRFLVGVIFGLLTSYAVESAVKSGRNVLVGFTTAGWPIGWVISYVAYVLLKNWNVINISGILIMLLALFELNGKEFGERSKISVSFPRLTSILIYVSALTPAFILQVMPSFLEVIKLTWLILPSYLLSIPTYVLLPIISEKVGIRKVLVISSIIVILSCVITFLLLPMMVIVFTSIGLGILGITPKYLAIRGEDPKRMGLALNIGSVMGLILPVVYGLMPFSSEFLLSILMVLMILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WD9</accession>
    <name>Q97WD9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable acyl-coenzyme a thioester hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2287</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90399">
        <property type="entry name" value="B90399"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991827.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WD9"/>
    <dbReference type="SMR" id="Q97WD9"/>
    <dbReference type="FunCoup" id="Q97WD9">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2287"/>
    <dbReference type="PaxDb" id="273057-SSO2287"/>
    <dbReference type="EnsemblBacteria" id="AAK42449">
        <property type="protein sequence ID" value="AAK42449"/>
        <property type="gene ID" value="SSO2287"/>
    </dbReference>
    <dbReference type="GeneID" id="1453776"/>
    <dbReference type="KEGG" id="sso:SSO2287"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2380"/>
    <dbReference type="eggNOG" id="arCOG00773">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073524_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WD9"/>
    <dbReference type="PhylomeDB" id="Q97WD9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052816">
        <property type="term" value="F:long-chain fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040170">
        <property type="entry name" value="Cytosol_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049">
        <property type="entry name" value="ACYL COENZYME A THIOESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049:SF16">
        <property type="entry name" value="PROTEIN VDLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="2">
        <location>
            <begin position="7"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="HotDog ACOT-type" evidence="2">
        <location>
            <begin position="165"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="318" mass="36244" checksum="97D52EFD4CD33AE4" modified="2001-10-01" version="1">MSYLKISDTIVETFRFIHYEQSNFLNRLHGGDMLFFLVETGMISASKVAMGTTLLASLDDVVFKKPVRLGDIVKVRAKTVYIGNTSLEVEVRAFVRDEEVVSAYATYVKVDDLLRPTPVNVKIMAESEDDKRKIDEAKKRRENRLSKIVNRQKMRFYVDDITDGLRYRISNVIHVSPELTYDGRIMSAGKLLKLMDDLGGIICLRYLDHNSSNLYDNSFNAVVTVAVKGLAFYSPIRLNDIILIRAGLVYVGNTSADILINVVREDLNGAKEHVATAYFTYVRVDKDGKPIKIPEYTPVTEREKRLYEEALTRRGLRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2015-10-14" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WH1</accession>
    <name>NURA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA double-strand break repair nuclease NurA</fullName>
            <ecNumber evidence="4">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nurA</name>
        <name evidence="8" type="ordered locus">SSO2248</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2014" name="FEBS Lett." volume="588" first="4637" last="4644">
            <title>Molecular architecture of the HerA-NurA DNA double-strand break resection complex.</title>
            <authorList>
                <person name="Byrne R.T."/>
                <person name="Schuller J.M."/>
                <person name="Unverdorben P."/>
                <person name="Foerster F."/>
                <person name="Hopfner K.P."/>
            </authorList>
            <dbReference type="PubMed" id="25447518"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2014.10.035"/>
        </citation>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH HERA</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="journal article" date="2012" name="Nucleic Acids Res." volume="40" first="3183" last="3196">
            <title>Structural and functional insights into DNA-end processing by the archaeal HerA helicase-NurA nuclease complex.</title>
            <authorList>
                <person name="Blackwood J.K."/>
                <person name="Rzechorzek N.J."/>
                <person name="Abrams A.S."/>
                <person name="Maman J.D."/>
                <person name="Pellegrini L."/>
                <person name="Robinson N.P."/>
            </authorList>
            <dbReference type="PubMed" id="22135300"/>
            <dbReference type="DOI" id="10.1093/nar/gkr1157"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS)</scope>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH HERA</scope>
        <scope>MUTAGENESIS OF LYS-202; ILE-295 AND PHE-300</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2015" name="PLoS ONE" volume="10" first="e0142345" last="e0142345">
            <title>NurA Is Endowed with Endo- and Exonuclease Activities that Are Modulated by HerA: New Insight into Their Role in DNA-End Processing.</title>
            <authorList>
                <person name="De Falco M."/>
                <person name="Catalano F."/>
                <person name="Rossi M."/>
                <person name="Ciaramella M."/>
                <person name="De Felice M."/>
            </authorList>
            <dbReference type="PubMed" id="26560692"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0142345"/>
        </citation>
        <scope>FUNCTION AS AN ENDONUCLEASE</scope>
        <scope>FUNCTION AS AN EXONUCLEASE</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>DNA-BINDING</scope>
        <scope>MUTAGENESIS OF ASP-58</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2018" name="Extremophiles" volume="22" first="581" last="589">
            <title>The Sulfolobus solfataricus RecQ-like DNA helicase Hel112 inhibits the NurA/HerA complex exonuclease activity.</title>
            <authorList>
                <person name="De Falco M."/>
                <person name="Massa F."/>
                <person name="Rossi M."/>
                <person name="De Felice M."/>
            </authorList>
            <dbReference type="PubMed" id="29488113"/>
            <dbReference type="DOI" id="10.1007/s00792-018-1018-7"/>
        </citation>
        <scope>FUNCTION AS AN ENDO- AND EXONUCLEASE</scope>
        <scope>ACTIVITY REGULATION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2 4">Involved in DNA double-strand break (DSB) repair (PubMed:22135300). Probably acts with HerA to stimulate resection of the 5' strand and produce the long 3' single-strand that is required for RadA loading (By similarity). NurA and HerA together stimulate the end-resection of six nucleotides of a linear DNA substrate (PubMed:26560692). Processes linear double-stranded (ds)DNA probes with 3' or 5' single-stranded overhangs or blunt ends (PubMed:22135300, PubMed:26560692). Has endonuclease activity on single-stranded (ss)DNA and nicking activity on dsDNA without HerA as well as 5'- and 3'-exonuclease activity on ssDNA (PubMed:26560692). Binds ssDNA, dsDNA, forked and bubble DNA equally well (PubMed:26560692).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="2 4">Nuclease activity requires the presence of HerA (PubMed:22135300). Another report shows endo- and exonuclease activity in the absence of HerA; HerA stimulates the exo- but not endonuclease (PubMed:26560692, PubMed:29488113). LhrC-Core (Hel112) inhibits the exonuclease activity of the HerA-NurA complex on ss- and dsDNA, has no effect on the nicking activity of NurA (PubMed:29488113). Endo- and exonuclease activities are inhibited by ATP; ATP may subtract divalent ions from the reaction preventing nuclease activity, HerA can alleviate ATP inhibition (PubMed:26560692).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3 4 5">Homodimer (PubMed:22135300, PubMed:25447518, PubMed:26560692). Forms a complex with HerA (PubMed:22135300, PubMed:25447518, PubMed:26560692).</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-10110462">
            <id>Q97WH1</id>
        </interactant>
        <interactant intactId="EBI-10110451">
            <id>Q97WG8</id>
            <label>herA</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>3</experiments>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NurA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90395">
        <property type="entry name" value="A90395"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991544.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2YGK">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B=1-339"/>
    </dbReference>
    <dbReference type="PDBsum" id="2YGK"/>
    <dbReference type="AlphaFoldDB" id="Q97WH1"/>
    <dbReference type="EMDB" id="EMD-2808"/>
    <dbReference type="SMR" id="Q97WH1"/>
    <dbReference type="IntAct" id="Q97WH1">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="MINT" id="Q97WH1"/>
    <dbReference type="STRING" id="273057.SSO2248"/>
    <dbReference type="PaxDb" id="273057-SSO2248"/>
    <dbReference type="EnsemblBacteria" id="AAK42416">
        <property type="protein sequence ID" value="AAK42416"/>
        <property type="gene ID" value="SSO2248"/>
    </dbReference>
    <dbReference type="GeneID" id="1453743"/>
    <dbReference type="GeneID" id="44127982"/>
    <dbReference type="KEGG" id="sso:SSO2248"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2343"/>
    <dbReference type="eggNOG" id="arCOG00367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_835811_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WH1"/>
    <dbReference type="EvolutionaryTrace" id="Q97WH1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053461">
        <property type="entry name" value="DSB_repair_nuclease_NurA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018977">
        <property type="entry name" value="NurA_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041033">
        <property type="entry name" value="NurA_Sulf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09376">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00933">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000434029" description="DNA double-strand break repair nuclease NurA">
        <location>
            <begin position="1"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Loss of exonuclease but not endonuclease activity." evidence="4">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Interacts with HerA but lacks activity." evidence="2">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Does not interact with HerA. Does not affect dimerization. Strong decrease in nucleolytic activity." evidence="2">
        <original>I</original>
        <variation>E</variation>
        <location>
            <position position="295"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Decreases interaction with HerA. Does not affect dimerization. Decreases nucleolytic activity." evidence="2">
        <original>F</original>
        <variation>E</variation>
        <location>
            <position position="300"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="3"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="39"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="124"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="127"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="136"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="151"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="162"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="194"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="197"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="turn" evidence="10">
        <location>
            <begin position="208"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="212"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="216"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="226"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="233"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="248"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="270"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="280"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q8U1N8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="22135300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="25447518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="26560692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="29488113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="6">
        <source>
            <dbReference type="PubMed" id="22135300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <evidence type="ECO:0000312" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="2YGK"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="2YGK"/>
        </source>
    </evidence>
    <sequence length="339" mass="39125" checksum="ACF81EEF43C015A8" modified="2001-10-01" version="1">MIRKIYDKLVESHNEIKNQIYNIANYLKQEIQDKVNEYWNEYVINHEQSETCKFVAIDGGSFGRPMRIGIVYAVGAESVIGDNKGVKTLSEDGQIGIFKPGNDAQERISLLMEALELSLALRDGSKGDYILMDGSLSKKIGNKVDIQQFSDEELKLIRNVDLNGIISIKDERKMRDLLMLLNQFLVSKIIEEYDGNVLWISKVSRGRDLFGTDYPDITVLELFTEKRGFSKLIIKNIDIEKISEIPEIEVLRKMEYTTFYTRLDNGKRVIRVDIVGRVDEKIVKEIMDRLSGVSIKGYPFPLLKAHMDVRFSAMDREKIIKLVGSKLHKDIEWWPSQFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WI8</accession>
    <name>Q97WI8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transport protein, hypothetical</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO2228</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90392">
        <property type="entry name" value="G90392"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992046.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WI8"/>
    <dbReference type="STRING" id="273057.SSO2228"/>
    <dbReference type="PaxDb" id="273057-SSO2228"/>
    <dbReference type="EnsemblBacteria" id="AAK42398">
        <property type="protein sequence ID" value="AAK42398"/>
        <property type="gene ID" value="SSO2228"/>
    </dbReference>
    <dbReference type="GeneID" id="1453725"/>
    <dbReference type="KEGG" id="sso:SSO2228"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2325"/>
    <dbReference type="eggNOG" id="arCOG01478">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076277_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WI8"/>
    <dbReference type="PhylomeDB" id="Q97WI8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF30">
        <property type="entry name" value="TRANSPORT PROTEIN, HYPOTHETICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="7">
        <location>
            <begin position="14"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK42398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="294" mass="33720" checksum="A06B83639B3C3E45" modified="2001-10-01" version="1">MISGKVLRRVSIIFLFSSISLIPIFLSELIAGILFKSYVLMADSYHTLIDFLMSFIFYFTLIKINTRSKRFPWGLYNLESLVVLTASIFIVYLSTNLILSMFDNAITLSISPWYSIILFVSSIYSLTLFLIERRYVEIQIVKNDMIHSLLDGVTEVVSGVTLILQNTVLMDTVTLLILAFTLSDVVREIKDSIVSILGASIDSPMKFQLINELRSKNIPIINLYLKKCGSFYAVYTYIGLPKDISLEKAYKIKRKTKRIIKKYDNIAYVDVILVPLTEIKKKKILEQVNVLYSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-07-05" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WJ1</accession>
    <name>RNP4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease P protein component 4</fullName>
            <shortName evidence="1">RNase P component 4</shortName>
            <ecNumber evidence="1">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Rpp21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnp4</name>
        <name type="ordered locus">SSO2225</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90392">
        <property type="entry name" value="D90392"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992052.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WJ1"/>
    <dbReference type="SMR" id="Q97WJ1"/>
    <dbReference type="STRING" id="273057.SSO2225"/>
    <dbReference type="PaxDb" id="273057-SSO2225"/>
    <dbReference type="EnsemblBacteria" id="AAK42395">
        <property type="protein sequence ID" value="AAK42395"/>
        <property type="gene ID" value="SSO2225"/>
    </dbReference>
    <dbReference type="GeneID" id="1453722"/>
    <dbReference type="KEGG" id="sso:SSO2225"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2321"/>
    <dbReference type="eggNOG" id="arCOG04345">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079140_3_1_2"/>
    <dbReference type="InParanoid" id="Q97WJ1"/>
    <dbReference type="PhylomeDB" id="Q97WJ1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00757">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016432">
        <property type="entry name" value="RNP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004878">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000153863" description="Ribonuclease P protein component 4">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00757"/>
        </source>
    </evidence>
    <sequence length="106" mass="12788" checksum="04A19EB3373346A3" modified="2001-10-01" version="1">MRRKNQIKKRIIELIELAYNTAKSGNLELAREYVKLAEMYSRKGKVEIPLKYKRMFCRKCYTPLIIGVTERRRMRSKILIRTCLICNWQRRYVLSGNKRSDKENES</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WJ2</accession>
    <name>Q97WJ2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">SSO10051</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90392">
        <property type="entry name" value="C90392"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992055.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WJ2"/>
    <dbReference type="SMR" id="Q97WJ2"/>
    <dbReference type="STRING" id="273057.SSO10051"/>
    <dbReference type="PaxDb" id="273057-SSO10051"/>
    <dbReference type="EnsemblBacteria" id="AAK42394">
        <property type="protein sequence ID" value="AAK42394"/>
        <property type="gene ID" value="SSO10051"/>
    </dbReference>
    <dbReference type="GeneID" id="1453721"/>
    <dbReference type="KEGG" id="sso:SSO10051"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2320"/>
    <dbReference type="eggNOG" id="arCOG01346">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095994_3_1_2"/>
    <dbReference type="InParanoid" id="Q97WJ2"/>
    <dbReference type="PhylomeDB" id="Q97WJ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000967">
        <property type="term" value="P:rRNA 5'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051925">
        <property type="entry name" value="RNA-binding_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40065">
        <property type="entry name" value="RNA-BINDING PROTEIN YHBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40065:SF3">
        <property type="entry name" value="RNA-BINDING PROTEIN YHBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRM" evidence="3">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK42394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="89" mass="10364" checksum="44976AD97ACE8FDE" modified="2001-10-01" version="1">MYSQEIKDLIKKTRAERADIRIGKYGVTEGMINEIKRRLNEHKVVKVKIGIKEIDDRKEFAKRVAEMVGAKLIEVRGYTFILAKIDSDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WK9</accession>
    <name>Q97WK9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO2207</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90390">
        <property type="entry name" value="B90390"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WK9"/>
    <dbReference type="STRING" id="273057.SSO2207"/>
    <dbReference type="PaxDb" id="273057-SSO2207"/>
    <dbReference type="EnsemblBacteria" id="AAK42377">
        <property type="protein sequence ID" value="AAK42377"/>
        <property type="gene ID" value="SSO2207"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2207"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2303"/>
    <dbReference type="eggNOG" id="arCOG07314">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1243075_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WK9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="222" mass="26098" checksum="76A0A9D1E08E3389" modified="2001-10-01" version="1">MKMSWIRDTSFLMECVKNENIKIEINSSNYFMSFNLLNGKYNLSLFSSHDIRISYDGNRLIDMHNLRVLKDNEARVHISNMIRNIKVNMSNEINNLAIMYNIPVKILYENLEAIFNLDFSLLSCLDYGLDYFLLHLTNNFAKQSSQFEVVKKLKFILGNERGCIKAILSLSNTYESDSFLFSSDCINFQTDVNAFSKFLRDYRTLNVKYIEVIDYLKQRLPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WW4</accession>
    <name>Q97WW4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR-associated protein Csa5 (Type I-A)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1996</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90366">
        <property type="entry name" value="C90366"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923715.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WW4"/>
    <dbReference type="SMR" id="Q97WW4"/>
    <dbReference type="STRING" id="273057.SSO1996"/>
    <dbReference type="PaxDb" id="273057-SSO1996"/>
    <dbReference type="EnsemblBacteria" id="AAK42186">
        <property type="protein sequence ID" value="AAK42186"/>
        <property type="gene ID" value="SSO1996"/>
    </dbReference>
    <dbReference type="GeneID" id="1453513"/>
    <dbReference type="KEGG" id="sso:SSO1996"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2073"/>
    <dbReference type="eggNOG" id="arCOG03823">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1811533_0_0_2"/>
    <dbReference type="InParanoid" id="Q97WW4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09750">
        <property type="entry name" value="Csa5_I-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010157">
        <property type="entry name" value="CRISPR-assoc_Cas5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01878">
        <property type="entry name" value="cas_Csa5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09702">
        <property type="entry name" value="Cas_Csa5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14323" checksum="5F84784BE69B860B" modified="2001-10-01" version="1">MRVANLLAVGFLYSESPTLVDRFANALSKEAVTKVLYDVQRIVQMGIDRGEIVSTTTMIQGKEYPAVNVSSSEGKYTVVGYLPTNQDIEYFLRMIEEDVYYARKAGALAMSIANRTKLVSKSEQGGEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97WZ0</accession>
    <name>Q97WZ0_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1969</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90363">
        <property type="entry name" value="B90363"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991918.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97WZ0"/>
    <dbReference type="PaxDb" id="273057-SSO1969"/>
    <dbReference type="EnsemblBacteria" id="AAK42161">
        <property type="protein sequence ID" value="AAK42161"/>
        <property type="gene ID" value="SSO1969"/>
    </dbReference>
    <dbReference type="GeneID" id="54122934"/>
    <dbReference type="KEGG" id="sso:SSO1969"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2045"/>
    <dbReference type="eggNOG" id="arCOG02221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135601_2_0_2"/>
    <dbReference type="InParanoid" id="Q97WZ0"/>
    <dbReference type="PhylomeDB" id="Q97WZ0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18689">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677:SF4">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="5"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="132" mass="15036" checksum="1CB09DEB0E0258C8" modified="2001-10-01" version="1">MRRKILFDTGFFHVYFSGLNEEAKKAVEEVYTGKSVGYTLDLNLAEFLYTYGKLNGVEEANVRLSLILNSPIKIVSTNKELALRAGELKVKYQNLSIVDCFLVAFAEKENTVIYTTDSEIKRVYKNTIILHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X31</accession>
    <name>Q97X31_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1922</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90357">
        <property type="entry name" value="F90357"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988444.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X31"/>
    <dbReference type="PaxDb" id="273057-SSO1922"/>
    <dbReference type="EnsemblBacteria" id="AAK42117">
        <property type="protein sequence ID" value="AAK42117"/>
        <property type="gene ID" value="SSO1922"/>
    </dbReference>
    <dbReference type="GeneID" id="7807172"/>
    <dbReference type="KEGG" id="sso:SSO1922"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1994"/>
    <dbReference type="eggNOG" id="arCOG00726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121774_2_1_2"/>
    <dbReference type="InParanoid" id="Q97X31"/>
    <dbReference type="PhylomeDB" id="Q97X31"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09873">
        <property type="entry name" value="PIN_Pae0151-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044153">
        <property type="entry name" value="PIN_Pae0151-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051619">
        <property type="entry name" value="TypeII_TA_RNase_PINc/VapC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901:SF1">
        <property type="entry name" value="EXONUCLEASE VAPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901">
        <property type="entry name" value="RIBONUCLEASE VAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="2">
        <location>
            <begin position="3"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="131" mass="14935" checksum="5FC6E487816F5E46" modified="2001-10-01" version="1">MRVIDSSSLVKFFSKEKGWEKVVEIISEGVMTLDLSIKEVANSLWKKILLGEMKEDVVIKILSDLLKREALLIVSQDEYLIEAFKIANRNKITVYDSLFIALAKSNNLELVTSDKKQYEVAIKEGVNTRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="138" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X64</accession>
    <name>Q97X64_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ATP-dependent RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO1889</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99353">
        <property type="entry name" value="A99353"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992772.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X64"/>
    <dbReference type="FunCoup" id="Q97X64">
        <property type="interactions" value="112"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1889"/>
    <dbReference type="PaxDb" id="273057-SSO1889"/>
    <dbReference type="EnsemblBacteria" id="AAK42080">
        <property type="protein sequence ID" value="AAK42080"/>
        <property type="gene ID" value="SSO1889"/>
    </dbReference>
    <dbReference type="GeneID" id="1453407"/>
    <dbReference type="KEGG" id="sso:SSO1889"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1945"/>
    <dbReference type="eggNOG" id="arCOG00558">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_3_2"/>
    <dbReference type="InParanoid" id="Q97X64"/>
    <dbReference type="PhylomeDB" id="Q97X64"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="29"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="197"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK42080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="343" mass="38702" checksum="4978655596931443" modified="2001-10-01" version="1">MFENLSEDLRKALSEAGYIKPTKVQEVVIPELLNGKSVIAQAKTGSGKTAAYVIPILERNSTALILSPTRELATQILDEIRKLGKYKQIDVSLIIGGMSYDGQRQSRIVVGTPGRLLDLWSKGKIDFSGYDVIIVDEADRMLDMGFIDDIRMILNHSNAKITGFFSATIPDEIMTLAKEFSKDLREIVLDEYKPVEEVKQIFVKVRNDWSDKVSKLLEEISGEKILVFARTRDRARKLYFLLRGKGVNVGLLSGDMPQSVRLKNFYGFKKGKYNVLVATDLASRGIDIVDVDKVINFDIPRDVETYIHRVGRTGRMGRVGQAVTFYTFREADMIKRINNLLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="119" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X92</accession>
    <name>Q97X92_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Multidrug-efflux transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO1863</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90349">
        <property type="entry name" value="C90349"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988517.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X92"/>
    <dbReference type="STRING" id="273057.SSO1863"/>
    <dbReference type="PaxDb" id="273057-SSO1863"/>
    <dbReference type="EnsemblBacteria" id="AAK42050">
        <property type="protein sequence ID" value="AAK42050"/>
        <property type="gene ID" value="SSO1863"/>
    </dbReference>
    <dbReference type="GeneID" id="1453377"/>
    <dbReference type="KEGG" id="sso:SSO1863"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1912"/>
    <dbReference type="eggNOG" id="arCOG00132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025894_3_1_2"/>
    <dbReference type="InParanoid" id="Q97X92"/>
    <dbReference type="PhylomeDB" id="Q97X92"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520:SF5">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="272"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="357"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="5"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="398" mass="42744" checksum="2DB8614DF5B6FA8E" modified="2001-10-01" version="1">MNKISVFSLTISRILRSLSAGIIFVILPYLVLVELKYSSLILGFIYTVGTFATAILGLAVGYLADLYGRKNSLILVSLFLPLSVLLIFINHSLIALFIASALGGYSATGAVAGGGIGGIVAPIQNALITELTDNEDRTFYFSLFTFLSGISASIGSLVAGFFTSKQGFLFAIILGFLSVLALFPIRAKNIKARSASLKSKVVIGKFSITGLLNGISVGLITPFLIPYFIIVYHTPKSEMSIYTFLSSLIASTIILLAPVLDKKIGFLKSIAITRGIGALLSIIMPLIRVFPISLGIYLILPGMRVLALPIQQRAMTEMVSQDEVGRAMGINQVTRLAASSGSTGLTGYLFSESQIDVPFLASGIIMALNIYMYYKFFGGKNEVNRPSRGSSLLKPTRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XA2</accession>
    <name>Q97XA2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Second ORF in transposon ISC1359</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO1847</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99348">
        <property type="entry name" value="A99348"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XA2"/>
    <dbReference type="STRING" id="273057.SSO1847"/>
    <dbReference type="PaxDb" id="273057-SSO1847"/>
    <dbReference type="EnsemblBacteria" id="AAK42040">
        <property type="protein sequence ID" value="AAK42040"/>
        <property type="gene ID" value="SSO1847"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1847"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881170_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XA2"/>
    <dbReference type="PhylomeDB" id="Q97XA2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase IS4-like" evidence="2">
        <location>
            <begin position="38"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="148" mass="17561" checksum="2AFC48F8BE0181EC" modified="2001-10-01" version="1">MAELDISGFLCVRHGSGAYLIILRKEDDKIIAFLVRREMDLYDAIVLAEMYRERWGIENAFRSLEEFRIRTRTCDVRKELVLVLLSYLLLNVWFLIRSWRKVKLWEFSVSLSNLLDREVRVEPERAFREVKTSFPQTPANPMHLLPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XF8</accession>
    <name>Q97XF8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1784</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90340">
        <property type="entry name" value="A90340"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989710.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XF8"/>
    <dbReference type="PaxDb" id="273057-SSO1784"/>
    <dbReference type="EnsemblBacteria" id="AAK41976">
        <property type="protein sequence ID" value="AAK41976"/>
        <property type="gene ID" value="SSO1784"/>
    </dbReference>
    <dbReference type="GeneID" id="1454755"/>
    <dbReference type="KEGG" id="sso:SSO1784"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1834"/>
    <dbReference type="eggNOG" id="arCOG07308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754798_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XF8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="148" mass="16203" checksum="5F66F178B9A91901" modified="2001-10-01" version="1">MKKHIRYIIDGYSTLREADIKNVLKSLLSNLDDDVRVSKKGYKISLSFRTPDNVRIMQCEEIDELLSRIGVMVTRIIVSSVITYEYEGAGVGATVGGIVGGGSTKRLDVGLLIAVLSGLAGYSVGKLIEKGEAILLSCEKRNGRWVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XJ5</accession>
    <name>Q97XJ5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90335">
        <property type="entry name" value="B90335"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990354.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XJ5"/>
    <dbReference type="PaxDb" id="273057-SSO1740"/>
    <dbReference type="EnsemblBacteria" id="AAK41937">
        <property type="protein sequence ID" value="AAK41937"/>
        <property type="gene ID" value="SSO1740"/>
    </dbReference>
    <dbReference type="GeneID" id="1454716"/>
    <dbReference type="KEGG" id="sso:SSO1740"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1785"/>
    <dbReference type="eggNOG" id="arCOG03724">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1718297_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XJ5"/>
    <dbReference type="PhylomeDB" id="Q97XJ5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58064">
        <property type="entry name" value="Influenza hemagglutinin (stalk)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="152" mass="18229" checksum="B03FB045A7341C42" modified="2001-10-01" version="1">MADDILYRTLGELLSNYVKTLLEKSGQGKRLSEQEYLILMNYYNSKNMDYVFTELRNIRNELRGIMEIIDRKSKEAIDYADKSRKEAMEYAEKNKKEVIDYVDKTKKEMLDYVDKRFEAIDKRFEAIDKRFDDLNNRIDDLRELILTLAKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XM4</accession>
    <name>Q97XM4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1689</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90330">
        <property type="entry name" value="D90330"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XM4"/>
    <dbReference type="STRING" id="273057.SSO1689"/>
    <dbReference type="PaxDb" id="273057-SSO1689"/>
    <dbReference type="EnsemblBacteria" id="AAK41899">
        <property type="protein sequence ID" value="AAK41899"/>
        <property type="gene ID" value="SSO1689"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1689"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1741"/>
    <dbReference type="eggNOG" id="arCOG07734">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2191145_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XM4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="124" mass="14383" checksum="08C69A2CF9B553C6" modified="2001-10-01" version="1">MVEHHCFKYLYGKTILMELEKYAKFLEDYANFVKNNKPIINIPLSPEELLSEASRIRALSRVKRENGLIIVKLAEGNAEHWAHFEGEVIMTFDNLYRPLKIEIEIKDTMDSEKVLKDANLIGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XT2</accession>
    <name>Q97XT2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99323">
        <property type="entry name" value="A99323"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923527.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XT2"/>
    <dbReference type="PaxDb" id="273057-SSO1625"/>
    <dbReference type="EnsemblBacteria" id="AAK41840">
        <property type="protein sequence ID" value="AAK41840"/>
        <property type="gene ID" value="SSO1625"/>
    </dbReference>
    <dbReference type="GeneID" id="1454619"/>
    <dbReference type="KEGG" id="sso:SSO1625"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1668"/>
    <dbReference type="eggNOG" id="arCOG07244">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1275385_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XT2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="216" mass="25276" checksum="53F7133C8F97F7B0" modified="2001-10-01" version="1">MRLRDSLEKLPSIILNPITLSPIFYIDSEGNLFVVNFTEQFEKQAEEYVKYFLNYKYGNKIYDRIKEKIIRCAKENINKCENLSSDEFVALIDANRFMAQRSWISANDISIHAHLRGALLALLDNVVLIRLYNPFYSDYKTLKDLEGVYAITKIFLYYLNDSLLRKLANVSLNDAIERIANDLPKDKYNTPLIDFYTPFIFDVDDKILPPKLLLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XX7</accession>
    <name>Q97XX7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">4Fe-4S ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1581</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99317">
        <property type="entry name" value="A99317"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923482.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XX7"/>
    <dbReference type="STRING" id="273057.SSO1581"/>
    <dbReference type="PaxDb" id="273057-SSO1581"/>
    <dbReference type="EnsemblBacteria" id="AAK41792">
        <property type="protein sequence ID" value="AAK41792"/>
        <property type="gene ID" value="SSO1581"/>
    </dbReference>
    <dbReference type="GeneID" id="1454571"/>
    <dbReference type="KEGG" id="sso:SSO1581"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1616"/>
    <dbReference type="eggNOG" id="arCOG03696">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022113_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XX7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051684">
        <property type="entry name" value="Electron_Trans/Redox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30176">
        <property type="entry name" value="FERREDOXIN-TYPE PROTEIN NAPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30176:SF3">
        <property type="entry name" value="FERREDOXIN-TYPE PROTEIN NAPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
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            <end position="113"/>
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        </location>
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            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
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        <location>
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            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="257"/>
        </location>
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            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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        </location>
    </feature>
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        <location>
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        </location>
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        <location>
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        </location>
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        <location>
            <begin position="518"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="72518" checksum="41FAB11EC4500A58" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XX8</accession>
    <name>Q97XX8_SACS2</name>
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        <submittedName>
            <fullName evidence="12">Molybdopterin oxidoreductase, molybdopterin binding subunit</fullName>
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    </protein>
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        <name evidence="12" type="ordered locus">SSO1580</name>
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    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mopterin_OxRdtase"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TETRATHIONATE REDUCTASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Molybdopterin"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TAT_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TAT"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7" id="KW-0408">Iron</keyword>
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    <keyword evidence="10" id="KW-0472">Membrane</keyword>
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    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK41791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="1050" mass="118373" checksum="83215B83B5829AD2" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y29</accession>
    <name>Q97Y29_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90310">
        <property type="entry name" value="G90310"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y29"/>
    <dbReference type="STRING" id="273057.SSO1520"/>
    <dbReference type="PaxDb" id="273057-SSO1520"/>
    <dbReference type="EnsemblBacteria" id="AAK41742">
        <property type="protein sequence ID" value="AAK41742"/>
        <property type="gene ID" value="SSO1520"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1520"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1561"/>
    <dbReference type="eggNOG" id="arCOG01191">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2079532_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y29"/>
    <dbReference type="PhylomeDB" id="Q97Y29"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="117" mass="13930" checksum="3420E14682494C64" modified="2001-10-01" version="1">MPFMWSKHYNYMLKLVIFELFGKEYEGHGIRELISYLSKLLKESEYEDLAKKINELIGEYRQQLIAIEDAYIDSGYENIEYESEDLKHLIEVTEIIVKFLEKVIKIVKLGLSSDFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y89</accession>
    <name>Q97Y89_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Csa3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1444</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90302">
        <property type="entry name" value="F90302"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923409.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y89"/>
    <dbReference type="SMR" id="Q97Y89"/>
    <dbReference type="STRING" id="273057.SSO1444"/>
    <dbReference type="PaxDb" id="273057-SSO1444"/>
    <dbReference type="EnsemblBacteria" id="AAK41677">
        <property type="protein sequence ID" value="AAK41677"/>
        <property type="gene ID" value="SSO1444"/>
    </dbReference>
    <dbReference type="GeneID" id="1454456"/>
    <dbReference type="KEGG" id="sso:SSO1444"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1473"/>
    <dbReference type="eggNOG" id="arCOG01446">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107894_2_0_2"/>
    <dbReference type="InParanoid" id="Q97Y89"/>
    <dbReference type="PhylomeDB" id="Q97Y89"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010163">
        <property type="entry name" value="Csa3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054588">
        <property type="entry name" value="Csa3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01884">
        <property type="entry name" value="cas_HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22662">
        <property type="entry name" value="Csa3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Csa3 N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="203" mass="23039" checksum="80183AADF76CE9C2" modified="2001-10-01" version="1">MILILTLGFDEKFQYRALMRQGKNVDKVIIIGGFEEEKAKKALESLVNFLRTVNVPFEIVEVDPRDFENVIENVGKVIVNNAGKDFMVNLSGGMRLMILAVFSAFLLTGIDATIEIETEDLTKVYYFRVSDVTSPSLFLTKAHLTILKAIKDGYSSANMISKTTGIPLTTTWRRLNELRKEKFIDEFNKLTIKGEILLKIYGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Y95</accession>
    <name>Q97Y95_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90301">
        <property type="entry name" value="H90301"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923403.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Y95"/>
    <dbReference type="STRING" id="273057.SSO1438"/>
    <dbReference type="PaxDb" id="273057-SSO1438"/>
    <dbReference type="EnsemblBacteria" id="AAK41671">
        <property type="protein sequence ID" value="AAK41671"/>
        <property type="gene ID" value="SSO1438"/>
    </dbReference>
    <dbReference type="GeneID" id="1454450"/>
    <dbReference type="KEGG" id="sso:SSO1438"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1467"/>
    <dbReference type="eggNOG" id="arCOG07299">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_848874_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Y95"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09724">
        <property type="entry name" value="CsaX_III-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022297">
        <property type="entry name" value="CRISPR-assoc_prot_CsaX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03876">
        <property type="entry name" value="cas_csaX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="359" mass="40165" checksum="A6DCE6D86E8E43EB" modified="2001-10-01" version="1">MEVPLYNIFGDNYVIQVATEAGNATIYNNKVEIEDDELRNVLNLAYKIAKNNEDAAAERRGKAKKKKGEEGETATSNLIIPLSGNDKNPWSETLKCYNFPTTVALSDVFKNFSQVNECEEVSAPSFVKPEFYEFGRSPGMVERARRVKLEVEPHYLIIAAAGWVLTRLGKAKVSEGDYVGVNVFTTTRGILYSLIQNVNGIIPGIKPETAFGLWIARKVVSSVTNPTVSVVRIYTMSDAIGQNPTTINGGFSIDLTKLLEKKDLLTERLEAIARNALSISSNMREIARNALSISSNMRERYIVLANYIYEYLTGSKRLEDLLYFANRDLIMNLNSNDEKVRDLKLISSYVNGELMKGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2023-11-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YH3</accession>
    <name>Q97YH3_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1904</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1348</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99291">
        <property type="entry name" value="A99291"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YH3"/>
    <dbReference type="STRING" id="273057.SSO1348"/>
    <dbReference type="PaxDb" id="273057-SSO1348"/>
    <dbReference type="EnsemblBacteria" id="AAK41584">
        <property type="protein sequence ID" value="AAK41584"/>
        <property type="gene ID" value="SSO1348"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1348"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1354"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1159101_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YH3"/>
    <dbReference type="PhylomeDB" id="Q97YH3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="1">
        <location>
            <begin position="172"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="239" mass="28526" checksum="5513EE8E81E0E479" modified="2001-10-01" version="1">MHFTYSIKNSEREKSKELIKEYRTLLQKAIDYLWNLTKIQVRKKNGNYKITLPKKKEVYKPLREELEKINHLASHYVDKAINDAFSILKSWRKRAIKGRASIEKPRVKKAYVRIKTTLRKVVGESVRITVRPHEYITFSWSKSWFSRRVKELELGEPIIKEEKVYLPFRYKLPWVTPVNFLAIDSNLYTLDAYDGEKFVTISLKQLYSLKYSMEVKRAKVQSFASKHTKRERVDEEVFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YQ2</accession>
    <name>Q97YQ2_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">6-phospho-N-acetylmuramidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90281">
        <property type="entry name" value="F90281"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990030.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YQ2"/>
    <dbReference type="STRING" id="273057.SSO1272"/>
    <dbReference type="PaxDb" id="273057-SSO1272"/>
    <dbReference type="EnsemblBacteria" id="AAK41509">
        <property type="protein sequence ID" value="AAK41509"/>
        <property type="gene ID" value="SSO1272"/>
    </dbReference>
    <dbReference type="GeneID" id="1454288"/>
    <dbReference type="KEGG" id="sso:SSO1272"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1268"/>
    <dbReference type="eggNOG" id="arCOG06045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065324_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YQ2"/>
    <dbReference type="PhylomeDB" id="Q97YQ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008589">
        <property type="entry name" value="MupG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043797">
        <property type="entry name" value="MupG_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38435">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38435:SF2">
        <property type="entry name" value="DUF871 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19200">
        <property type="entry name" value="MupG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phospho-N-acetylmuramidase N-terminal" evidence="1">
        <location>
            <begin position="6"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="335" mass="38920" checksum="570894F6C59631E0" modified="2001-10-01" version="1">MTKRRIGFSIFPGWKEIKDEQISLLKNARDLGFSEIFMGIGPGTHWRTPVNEALSVAKEILKEANKLDYYAFVDINPQILKELNASPRNLSMFMEIGFKGVRADYGFSKEEIVEMSKQMTVELNPFEISEEEVDFIARNANLERIKACHNYYPVLYSGISKEIFIEKNRIFKERGIEIGAFISNPKFNLRTTLEVLRYVEPFDSANYLFKFVDRVLIGDPIPDYESLRQVSEVFKSDITKIRIKVYDESVGKRLDKKFAVENYREFAIVCYTRDNIYEGETCYTKLFKNAVAVRGREVWIFTRDLGIGPYRLVGEIDDINMEIVKMSSEIMLISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YR7</accession>
    <name>Q97YR7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1252</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90279">
        <property type="entry name" value="D90279"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YR7"/>
    <dbReference type="PaxDb" id="273057-SSO1252"/>
    <dbReference type="EnsemblBacteria" id="AAK41491">
        <property type="protein sequence ID" value="AAK41491"/>
        <property type="gene ID" value="SSO1252"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1252"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1246"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123170_1_0_2"/>
    <dbReference type="InParanoid" id="Q97YR7"/>
    <dbReference type="PhylomeDB" id="Q97YR7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="7"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="117" mass="13745" checksum="591BB5CF9941B656" modified="2001-10-01" version="1">MLMSFLLNNAKNFLHDARRDFEEGIWNLSVFHSEQALQLCVKYKLYIHLGDYPKTHDLKVLINSLGRFEKVEIDELMLDFLVQSYISSRYLPYTFSKESAEKALNFVENMMRGLGCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YT8</accession>
    <name>Q97YT8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH bat-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90276">
        <property type="entry name" value="C90276"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923257.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YT8"/>
    <dbReference type="PaxDb" id="273057-SSO1219"/>
    <dbReference type="EnsemblBacteria" id="AAK41466">
        <property type="protein sequence ID" value="AAK41466"/>
        <property type="gene ID" value="SSO1219"/>
    </dbReference>
    <dbReference type="GeneID" id="1454245"/>
    <dbReference type="KEGG" id="sso:SSO1219"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1220"/>
    <dbReference type="eggNOG" id="arCOG02272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104910_1_0_2"/>
    <dbReference type="InParanoid" id="Q97YT8"/>
    <dbReference type="PhylomeDB" id="Q97YT8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH bat-type" evidence="1">
        <location>
            <begin position="151"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="228" mass="27188" checksum="C3D75FFFBBC4BA61" modified="2001-10-01" version="1">MSDRLLEITFSVEHENCWTNLVGKYVVKTLRFSVDTERNFIRSLIVVDKKYKELINKIKRNNSFIGYSLVSLTTDNKKILFDFRKRYKNSVMDNINSVDGIILDGFKYDGKEYWRVLLYESYVNELREKLKSKGNVEFISFHELDITEDELTPYELKTLILAYKNGYFDFPRRIKSDKISKLINISKSTFTYHLRSAESRIIKRYIDDLKFYNVINNVSKQEEEKKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YT9</accession>
    <name>Q97YT9_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">methylmalonate-semialdehyde dehydrogenase (CoA acylating)</fullName>
            <ecNumber evidence="1">1.2.1.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO1218</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.1.27" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90276">
        <property type="entry name" value="B90276"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923256.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YT9"/>
    <dbReference type="FunCoup" id="Q97YT9">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1218"/>
    <dbReference type="PaxDb" id="273057-SSO1218"/>
    <dbReference type="EnsemblBacteria" id="AAK41465">
        <property type="protein sequence ID" value="AAK41465"/>
        <property type="gene ID" value="SSO1218"/>
    </dbReference>
    <dbReference type="GeneID" id="1454244"/>
    <dbReference type="KEGG" id="sso:SSO1218"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1219"/>
    <dbReference type="eggNOG" id="arCOG01252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_10_2"/>
    <dbReference type="InParanoid" id="Q97YT9"/>
    <dbReference type="PhylomeDB" id="Q97YT9"/>
    <dbReference type="BRENDA" id="1.2.1.27">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004491">
        <property type="term" value="F:methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07085">
        <property type="entry name" value="ALDH_F6_MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000002">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase (Acylating)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000003">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase [acylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010061">
        <property type="entry name" value="MeMal-semiAld_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01722">
        <property type="entry name" value="MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866">
        <property type="entry name" value="MALONATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866:SF4">
        <property type="entry name" value="MALONATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="4">
        <location>
            <begin position="24"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="492" mass="54605" checksum="57B9D0558AF43284" modified="2001-10-01" version="1">MGKVLGEVKKDYGKLKLYINGEFIDSKTDTIGKAYNPAKDEIIAEVPFSAKDEVEEAIQSAQEAFEKWREVPITTRIQYLFALKNRLEEYSETIARIIVQNHGKTIQEARGDMRRTIENVEAAISAAYTLYKGEHLDQVSQEVDETVVREPLGVFGIITPFNFPTMVPFWFLPYAIVLGNTVVVKPSEITPVPMDFIIRIFDEIKLPRGVVNVVHGAKDVVDEFLTNKLVQGVTFVGSTRVGKYIYENAGKNGKKAIVQAGAKNFVVVMPDADLNKAIPSIVSAFFGNAGQRCLAAANLVAVGNIYDEVKRKFIEASKQLKIGYGLDESVDMGPVVTKDAKKRIIGYIEKGIEEGAKLLLDGRDVKVPEYPNGYFLGPTVFDEVTPEMVIAKEEIFGPVASIIHVKNLDEAINIINRSNYGNASSIFTTSGYYARKFRREVNTGNIGINIGVAAPMAFFPFGGRKESFFGILHGQIDSVDFFTDKKVVITRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YZ7</accession>
    <name>Q97YZ7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metalloprotease TldD/E C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90268">
        <property type="entry name" value="D90268"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923216.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YZ7"/>
    <dbReference type="STRING" id="273057.SSO1152"/>
    <dbReference type="PaxDb" id="273057-SSO1152"/>
    <dbReference type="EnsemblBacteria" id="AAK41403">
        <property type="protein sequence ID" value="AAK41403"/>
        <property type="gene ID" value="SSO1152"/>
    </dbReference>
    <dbReference type="GeneID" id="1454182"/>
    <dbReference type="KEGG" id="sso:SSO1152"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1146"/>
    <dbReference type="eggNOG" id="arCOG00322">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_4_1_2"/>
    <dbReference type="InParanoid" id="Q97YZ7"/>
    <dbReference type="PhylomeDB" id="Q97YZ7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43666:SF1">
        <property type="entry name" value="CONSERVED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43666">
        <property type="entry name" value="TLDD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="1">
        <location>
            <begin position="193"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="409" mass="46123" checksum="B5AB9BC6831AA522" modified="2001-10-01" version="1">MNEEMVKYVQEVKGEEVGLLRVKTRRIIIKLVESKVATIQRMTNSYYFLALKKDSQGFLGRINNLNEKPKLTPVDFFPIMSKNEREYKMVKNNTNILSLFDDLSPLLSLIQSEYPLYGIISIDDVERSLVTSKGFNGTESNLSISGYFRAKNKEHSGQWAFSSTSFSSSQVKDSIKKANELASITGKYEITEGKYDVILSPLVMGNLMESVARMASGYAIMSGMSMLKPGEKAGSDKFTLLDTPKEDRPNSWGFDDEGTFTYNKAIIENGVFTTPLLNNELSNVFKLPSTGNAGWIYPTAWNLEVKEGDTSFESLLSGNVVFINNVWYTRFQNYAEGDFSTVARDATVVYRNGNPVGVIGRVRIADNLKRILKNIVELSRERYSVRWWDAPMQGVYPYALVKDVRLSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z15</accession>
    <name>Q97Z15_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heterodisulfite reductase, subunit B (HdrB-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hdrB-1</name>
        <name evidence="2" type="ordered locus">SSO1129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90265">
        <property type="entry name" value="H90265"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989758.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z15"/>
    <dbReference type="FunCoup" id="Q97Z15">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1129"/>
    <dbReference type="PaxDb" id="273057-SSO1129"/>
    <dbReference type="EnsemblBacteria" id="AAK41383">
        <property type="protein sequence ID" value="AAK41383"/>
        <property type="gene ID" value="SSO1129"/>
    </dbReference>
    <dbReference type="GeneID" id="1454162"/>
    <dbReference type="KEGG" id="sso:SSO1129"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1123"/>
    <dbReference type="eggNOG" id="arCOG00342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052147_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z15"/>
    <dbReference type="PhylomeDB" id="Q97Z15"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43682">
        <property type="entry name" value="LACTATE UTILIZATION PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43682:SF1">
        <property type="entry name" value="LACTATE UTILIZATION PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cysteine-rich" evidence="1">
        <location>
            <begin position="233"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="437" mass="50700" checksum="238C73ECAF43E2BF" modified="2001-10-01" version="1">MLSQDEKQIQREVREAFPMSDDVDWNEVYQRIIYRYSTPHGLEHVKEEMYKLEDKGEIIIHHIKPYNNPVEAQTLNGSPKKIPTTKLWHHKSCGQCGHIPGYPTSVFWVMNKLEIDYLDEPHQTSCTGWNYHASGASNPVALAGVYVRNMWRAYETGYFPLIHCGTSFGHYKEVRNMIILHKEIRDKLRPIMRKLDMDIVIPEEVVHYSEWLYVMSKKAAQQKKYNLDNIKAAVHTPCHVYKLVPEDTVYDPEVFQGRRPAAPSGTVQNFGAKLVDYSTWWDCCGFGFRHILTEREFSRSFALFKKVIPAVEEGNADIFVTSDTGCVTTLDKSQWAGKAHGFNYNLPVLADAQFAALAMGADPYIIAQIHWHATDVEGFLRKIGVPVDDYKEKFVQYLQDLREGKTEPQYLYPKHRKIDFYLSLPDRVKWYKKEVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="139" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z19</accession>
    <name>Q97Z19_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Dihydrolipoamide dehydrogenase (PdhD-1)</fullName>
            <ecNumber evidence="12">1.8.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pdhD-1</name>
        <name evidence="12" type="ordered locus">SSO1123</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="12"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90265">
        <property type="entry name" value="C90265"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989754.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z19"/>
    <dbReference type="FunCoup" id="Q97Z19">
        <property type="interactions" value="249"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1123"/>
    <dbReference type="PaxDb" id="273057-SSO1123"/>
    <dbReference type="EnsemblBacteria" id="AAK41378">
        <property type="protein sequence ID" value="AAK41378"/>
        <property type="gene ID" value="SSO1123"/>
    </dbReference>
    <dbReference type="GeneID" id="1454157"/>
    <dbReference type="KEGG" id="sso:SSO1123"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1118"/>
    <dbReference type="eggNOG" id="arCOG01068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_2_2"/>
    <dbReference type="InParanoid" id="Q97Z19"/>
    <dbReference type="PhylomeDB" id="Q97Z19"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0274">FAD</keyword>
    <keyword evidence="3 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5 9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="11">
        <location>
            <begin position="3"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="10">
        <location>
            <begin position="327"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAK41378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="446" mass="48816" checksum="8BA32C48A3F20298" modified="2001-10-01" version="1">MKYDIVIIGGGTAGYVAGSILARKGKKVLVAEKEKFGGVCVNFGCVPSIFLFDATFLLNRFKEIVYYIGLDGEIEYKDLLFSKRNEIIDYLSNAGRKLIEDSGGETELGEVEIISPSTVKVNGRIVEFDNLIIATGSKPMVPSINGIENTLSEDDAVNLNSVPSSMVIIGGGYAGVEIAQMYSRLGSQVTLLSRSKILPTFPEDARSIIKDSLEFDGVNIEENIRIVKIHDGKVITEKGEVEGNVIVYATGRRPQLPKGIEILGLSINECGIVVDKYRRVKNNVYAIGDVIDKERKTAHSAILDAVIASLHILKDATFLPLIDNLKIPQVLYTDPQVGIVGNDKEAKEFSVFPFAATTRAIINGFKDGYVKLGINERNEIVFGEVIGDKAEELINILTLVVNNRIRIESLALMSFVHPSFSEAIVNAAKGFFDLDVDKYKSKDGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z54</accession>
    <name>Q97Z54_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">SSO1080</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC-4 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90260">
        <property type="entry name" value="G90260"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989716.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z54"/>
    <dbReference type="STRING" id="273057.SSO1080"/>
    <dbReference type="PaxDb" id="273057-SSO1080"/>
    <dbReference type="EnsemblBacteria" id="AAK41342">
        <property type="protein sequence ID" value="AAK41342"/>
        <property type="gene ID" value="SSO1080"/>
    </dbReference>
    <dbReference type="GeneID" id="1454121"/>
    <dbReference type="KEGG" id="sso:SSO1080"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1080"/>
    <dbReference type="eggNOG" id="arCOG02312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_2"/>
    <dbReference type="InParanoid" id="Q97Z54"/>
    <dbReference type="PhylomeDB" id="Q97Z54"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050250">
        <property type="entry name" value="Macrolide_Exporter_MacB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30572:SF4">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE YTRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30572">
        <property type="entry name" value="MEMBRANE COMPONENT OF TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="MacB-like periplasmic core" evidence="9">
        <location>
            <begin position="22"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="ABC3 transporter permease C-terminal" evidence="8">
        <location>
            <begin position="269"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK41342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="398" mass="42858" checksum="BD6C391604EE36C9" modified="2001-10-01" version="1">MKILDFISYALNSLKERKVRAILTILGIVVGPATIISINSMVLGYSHTIISQISNFLSPYDIIVTPTGRGLPLSQYLILQLETILGVKMVIPFYSFPALIRTPNGYEGATVFAVNINQLKIAAPAISLSSGYFPAAEVSYEASIGYQLGNPQGGYSPIRPNQVIQTIIFYNGNNFTKTFLVTGVLNEYGSFLGVDIDKSIIVPLSFGQSISSSYSGAIIIVSSLGEVNEVVNEIKQKFGNSLDIVVAEEFIQLIDNTLQSLNGLLVSAGATSFIVSFMGVTTTMFTTVVERTKEIGILRALGFTRFDVLTMFLVEASVMGFIGSITGLALGSVVALILTQEHFGLGFSFLKGLSVSPVYSPTFMLLVLIFSTILSVIAALGPAYNASKLDPNKALRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z73</accession>
    <name>Q97Z73_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">OsmC family peroxiredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90258">
        <property type="entry name" value="A90258"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989896.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z73"/>
    <dbReference type="SMR" id="Q97Z73"/>
    <dbReference type="STRING" id="273057.SSO1056"/>
    <dbReference type="PaxDb" id="273057-SSO1056"/>
    <dbReference type="DNASU" id="1454099"/>
    <dbReference type="EnsemblBacteria" id="AAK41320">
        <property type="protein sequence ID" value="AAK41320"/>
        <property type="gene ID" value="SSO1056"/>
    </dbReference>
    <dbReference type="GeneID" id="1454099"/>
    <dbReference type="KEGG" id="sso:SSO1056"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1052"/>
    <dbReference type="eggNOG" id="arCOG03686">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1860694_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z73"/>
    <dbReference type="PhylomeDB" id="Q97Z73"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052924">
        <property type="entry name" value="OsmC/Ohr_hydroprdx_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35368">
        <property type="entry name" value="HYDROPEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35368:SF1">
        <property type="entry name" value="HYDROPEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="OsmC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15293" checksum="C610FE907DABAC85" modified="2001-10-01" version="1">MTVITFTAEGRLDGDKVIVDLNGTEIKVGLLGSDNPTPEEFCLASAISCLILTVYYIAREKGVTINSIEGYIEGKMDTKGFQGVEEVPPGLLEVSYELIVYSKDSRISDILKEAEERCPMRDTLTRSVKVNINWKIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z74</accession>
    <name>Q97Z74_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90257">
        <property type="entry name" value="H90257"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989897.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z74"/>
    <dbReference type="STRING" id="273057.SSO1055"/>
    <dbReference type="PaxDb" id="273057-SSO1055"/>
    <dbReference type="EnsemblBacteria" id="AAK41319">
        <property type="protein sequence ID" value="AAK41319"/>
        <property type="gene ID" value="SSO1055"/>
    </dbReference>
    <dbReference type="GeneID" id="1454098"/>
    <dbReference type="KEGG" id="sso:SSO1055"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1051"/>
    <dbReference type="eggNOG" id="arCOG05920">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754796_0_0_2"/>
    <dbReference type="InParanoid" id="Q97Z74"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="148" mass="17427" checksum="805B14AD48526495" modified="2001-10-01" version="1">MKEKVGNIELEVEAIVEINGKDYKVVNVPNADEYKGFPPSWEFVKSHMLTWRPYFKAKMIEINNQLIPAVEDFLLNLDEDMYDLLLDIYYTFKVNKPSIETNISTVLTRQIDKLEEKFGRRFNEEEKTKLYIKYGIEVAILRDIGVIN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-20" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZH0</accession>
    <name>TRM1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.216</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA 2,2-dimethylguanosine-26 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA(guanine-26,N(2)-N(2)) methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA(m(2,2)G26)dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trm1</name>
        <name type="ordered locus">SSO0943</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90245">
        <property type="entry name" value="D90245"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992377.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZH0"/>
    <dbReference type="SMR" id="Q97ZH0"/>
    <dbReference type="FunCoup" id="Q97ZH0">
        <property type="interactions" value="276"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0943"/>
    <dbReference type="PaxDb" id="273057-SSO0943"/>
    <dbReference type="EnsemblBacteria" id="AAK41219">
        <property type="protein sequence ID" value="AAK41219"/>
        <property type="gene ID" value="SSO0943"/>
    </dbReference>
    <dbReference type="GeneID" id="1455186"/>
    <dbReference type="KEGG" id="sso:SSO0943"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.939"/>
    <dbReference type="eggNOG" id="arCOG01219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010862_5_1_2"/>
    <dbReference type="InParanoid" id="Q97ZH0"/>
    <dbReference type="PhylomeDB" id="Q97ZH0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160104">
        <property type="term" value="F:tRNA (guanine(26)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000272">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00290">
        <property type="entry name" value="tRNA_dimethyltr_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022923">
        <property type="entry name" value="TRM1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003331">
        <property type="entry name" value="PRK04338.1-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00308">
        <property type="entry name" value="TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF3">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000147693" description="tRNA (guanine(26)-N(2))-dimethyltransferase">
        <location>
            <begin position="1"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Trm1 methyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00290"/>
        </source>
    </evidence>
    <sequence length="378" mass="42874" checksum="1604FE962A88A0B2" modified="2001-10-01" version="1">MKLKEVTEGKVRIFVPDPTGYMVEGKFDPSWAPVFYNPKMTFNRDLSVIVVSILKPKIIVDALSATGIRGIRYYVESWKSEQLILNDKNPNATSLIQINAKNNGIENAKIYNKDANALLYEVKSDYIDIDPFGSPAPFILSSLNAATRNGIVAFTATDLSPLEGSSPTSCRRKYDAINHKLSSSKELGLRVLIGKIIREAAILEKTVYPLFSFYADYYYRLFVRVENGARKADDNINKHLKYFGECPRCGFQTFVEENCKTKCPVCGEIFSIIGPLYIGPLYSMEFLKRIMDLYSNFNYLTSFNRIQKLLNVIEKEARFKNVFYNISKLASKLKISAIPPIESILECLGDASRTHFAPTGIRTDKEYEEITKCIKSLR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-03" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZI9</accession>
    <name>GCSPB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable glycine dehydrogenase (decarboxylating) subunit 2</fullName>
            <ecNumber evidence="1">1.4.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glycine cleavage system P-protein subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycine decarboxylase subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycine dehydrogenase (aminomethyl-transferring) subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gcvPB</name>
        <name type="ordered locus">SSO0917</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvP family. C-terminal subunit subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90242">
        <property type="entry name" value="H90242"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992348.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZI9"/>
    <dbReference type="SMR" id="Q97ZI9"/>
    <dbReference type="FunCoup" id="Q97ZI9">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0917"/>
    <dbReference type="PaxDb" id="273057-SSO0917"/>
    <dbReference type="EnsemblBacteria" id="AAK41199">
        <property type="protein sequence ID" value="AAK41199"/>
        <property type="gene ID" value="SSO0917"/>
    </dbReference>
    <dbReference type="GeneID" id="1455166"/>
    <dbReference type="GeneID" id="44129847"/>
    <dbReference type="KEGG" id="sso:SSO0917"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.918"/>
    <dbReference type="eggNOG" id="arCOG00076">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004620_5_0_2"/>
    <dbReference type="InParanoid" id="Q97ZI9"/>
    <dbReference type="PhylomeDB" id="Q97ZI9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000224">
        <property type="entry name" value="Probable glycine dehydrogenase (decarboxylating) subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000014">
        <property type="entry name" value="Probable glycine dehydrogenase (decarboxylating) subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00713">
        <property type="entry name" value="GcvPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023012">
        <property type="entry name" value="GcvPB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049316">
        <property type="entry name" value="GDC-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003346">
        <property type="entry name" value="PRK04366.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="GLYCINE DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21478">
        <property type="entry name" value="GcvP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000167031" description="Probable glycine dehydrogenase (decarboxylating) subunit 2">
        <location>
            <begin position="1"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00713"/>
        </source>
    </evidence>
    <sequence length="508" mass="56742" checksum="65F18BCD42AB3185" modified="2001-10-01" version="1">MWRQAKWNEPLIFELNKSGAARQGFLINKDEEIRSQIKEMKIPKNLLRENEPDLPSLSELEVVRHFVRLSQMNFGVDIGIMPLGSCTMKYNPKIEEKATAITEFHHPLEDEDYIQGILEMIYELQNWFSEITGMDECSLQVPAGSAGEFAGVLMIKKYHEEHNRNYKDTILVADTAHGTNPASAAMAGYKVMYVKSNAEGLVDMDILREIVNDKTAGFMLTNPNTLGLFEENILEISKIIHSTNAVLYYDGANLNGVLGIARPGDMGFDIVHLNLHKTFAVPHGGGGPGAGAICAKGELVNYLPYPMVEKVNGKYKLSKIPKNSIGKIATFYGNVGNLARSFAYILGLGPQGIQMIGKMSTLATNYLIAKLRDVKELELIAPNRHRKHEVVFSVKQLMENYGVSANDVAKALLDNGFYAPTIYFPPIVEEALMIEPTETETKETLDMFAETLKKIVNDAKINPEQVMKSPNNTSIARLDQAYANHPSTITPTYRVLRLRRLGKIDYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZJ2</accession>
    <name>Q97ZJ2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90242">
        <property type="entry name" value="E90242"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992344.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZJ2"/>
    <dbReference type="STRING" id="273057.SSO0914"/>
    <dbReference type="PaxDb" id="273057-SSO0914"/>
    <dbReference type="EnsemblBacteria" id="AAK41196">
        <property type="protein sequence ID" value="AAK41196"/>
        <property type="gene ID" value="SSO0914"/>
    </dbReference>
    <dbReference type="GeneID" id="89231095"/>
    <dbReference type="KEGG" id="sso:SSO0914"/>
    <dbReference type="eggNOG" id="arCOG05982">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2340321_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZJ2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="102" mass="11610" checksum="C29FC53FDA7A62C1" modified="2001-10-01" version="1">MLAPSMASIVFLAYGLLSPIYSRFFKDKISNERLFLVAWSLAPHLVGLIYSPSFFIALLVLISLCVTLFIVYKGKFRIIYSGIIFLFMAVIIQIFINPLTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZL5</accession>
    <name>Q97ZL5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UbiD family decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO0879</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiD family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90238">
        <property type="entry name" value="F90238"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZL5"/>
    <dbReference type="FunCoup" id="Q97ZL5">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0879"/>
    <dbReference type="PaxDb" id="273057-SSO0879"/>
    <dbReference type="EnsemblBacteria" id="AAK41165">
        <property type="protein sequence ID" value="AAK41165"/>
        <property type="gene ID" value="SSO0879"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0879"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.886"/>
    <dbReference type="eggNOG" id="arCOG01671">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023348_4_1_2"/>
    <dbReference type="InParanoid" id="Q97ZL5"/>
    <dbReference type="PhylomeDB" id="Q97ZL5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1670.10">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049381">
        <property type="entry name" value="UbiD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049383">
        <property type="entry name" value="UbiD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048304">
        <property type="entry name" value="UbiD_Rift_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00148">
        <property type="entry name" value="UbiD family decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108">
        <property type="entry name" value="3-OCTAPRENYL-4-HYDROXYBENZOATE CARBOXY-LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108:SF17">
        <property type="entry name" value="FERULIC ACID DECARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01977">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20696">
        <property type="entry name" value="UbiD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20695">
        <property type="entry name" value="UbiD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143968">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like N-terminal" evidence="3">
        <location>
            <begin position="17"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like Rift-related" evidence="2">
        <location>
            <begin position="130"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like C-terminal" evidence="4">
        <location>
            <begin position="325"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK41165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="488" mass="55414" checksum="98964184DB5F462E" modified="2001-10-01" version="1">MLKYSNHMAFKDLREYIEFMKKKGKLIEVDDEVSVDLEIAEITRKATYAHLPPLLFKRVKNYENWKIVSNIFYSIESLYEIFGTNKLESISEGFLSNLSNMPITFFDKIKSLREILGLGKVMPKAKSPSFKEEKNLDLTKIPAIKTWPKDAGRYLTFSITITKDPETDVHNLSVYRVQILNEKEAIIHWQAFKRGALTAKKYLEKGISKIPIAVVTGVDPAIAFTAASPVPHGIDKYMFAGILRGEGIDVAELDNQLLVPSHSEVVLTGYVDLNDMRLEGPFGDHMGYYTPADYYPVFKLERVYIREDPIFHVTSVGKPPLEDAWIGKAVERIFLPFAKMLVPELIDMNLPEYGLFTGIGIFSIKKYYPGQAKRVMMALWGTGQLSLLKIIIVVDQDIDVHDINQVIYAIAANVDPKRDVWVIENALTDSLDPSVPFPPLGSKLGIDATRKFKEEMGKEWPEEVRSDEVVAKKADQILNKIIKRYQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZN8</accession>
    <name>Q97ZN8_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0846</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90235">
        <property type="entry name" value="E90235"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923135.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZN8"/>
    <dbReference type="STRING" id="273057.SSO0846"/>
    <dbReference type="PaxDb" id="273057-SSO0846"/>
    <dbReference type="EnsemblBacteria" id="AAK41140">
        <property type="protein sequence ID" value="AAK41140"/>
        <property type="gene ID" value="SSO0846"/>
    </dbReference>
    <dbReference type="GeneID" id="1455107"/>
    <dbReference type="KEGG" id="sso:SSO0846"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.862"/>
    <dbReference type="eggNOG" id="arCOG07250">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1406034_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZN8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="193" mass="22978" checksum="EACD38940A75D8A1" modified="2001-10-01" version="1">MIDLCVVKCDENEVKKKSKEIVEGLKEIYDNFNDSLIKEIRVEESVFGIRGSYNYNSKILTLYCINCVICVETIVHEIIHSNSYKRARDMYFEGLTEFLTLYYLKKRVRACLDHRFIDEICRINKEYEIYATFWGNLALIIGIKELWKYYSKGYNHNNIDNLVKNDIYKASFELAKRYNTKLMDLIDVIEKLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-02" modified="2026-01-28" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZQ6</accession>
    <name>RL34_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL34</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L34e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl34e</name>
        <name type="ordered locus">SSO6374</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90217">
        <property type="entry name" value="B90217"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991243.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZQ6"/>
    <dbReference type="SMR" id="Q97ZQ6"/>
    <dbReference type="FunCoup" id="Q97ZQ6">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO6374"/>
    <dbReference type="PaxDb" id="273057-SSO6374"/>
    <dbReference type="EnsemblBacteria" id="AAK40993">
        <property type="protein sequence ID" value="AAK40993"/>
        <property type="gene ID" value="SSO6374"/>
    </dbReference>
    <dbReference type="GeneID" id="1454960"/>
    <dbReference type="KEGG" id="sso:SSO6374"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.693"/>
    <dbReference type="eggNOG" id="arCOG04168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118652_2_0_2"/>
    <dbReference type="InParanoid" id="Q97ZQ6"/>
    <dbReference type="PhylomeDB" id="Q97ZQ6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00349">
        <property type="entry name" value="Ribosomal_eL34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_eL34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038562">
        <property type="entry name" value="Ribosomal_eL34_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018065">
        <property type="entry name" value="Ribosomal_eL34_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047868">
        <property type="entry name" value="Ribosomal_L34e_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003143">
        <property type="entry name" value="PRK04059.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01250">
        <property type="entry name" value="RIBOSOMALL34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01145">
        <property type="entry name" value="RIBOSOMAL_L34E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000131857" description="Large ribosomal subunit protein eL34">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="88" mass="10196" checksum="684C0CDDCC50E0D3" modified="2001-10-01" version="1">MPRPALRSRSLRRVYVKLPSGKTVIHYERKRNDIAICAICKKPLHGVKTNFLHKYSKSEKRPERPFGGYLCSSCLTQLIKATVRQQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZT4</accession>
    <name>Q97ZT4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF72 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0494</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90194">
        <property type="entry name" value="G90194"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923008.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZT4"/>
    <dbReference type="STRING" id="273057.SSO0494"/>
    <dbReference type="PaxDb" id="273057-SSO0494"/>
    <dbReference type="EnsemblBacteria" id="AAK40814">
        <property type="protein sequence ID" value="AAK40814"/>
        <property type="gene ID" value="SSO0494"/>
    </dbReference>
    <dbReference type="GeneID" id="1455624"/>
    <dbReference type="KEGG" id="sso:SSO0494"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.487"/>
    <dbReference type="eggNOG" id="arCOG04291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046519_0_1_2"/>
    <dbReference type="InParanoid" id="Q97ZT4"/>
    <dbReference type="PhylomeDB" id="Q97ZT4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.410">
        <property type="entry name" value="Protein of unknown function UPF0759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002763">
        <property type="entry name" value="DUF72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036520">
        <property type="entry name" value="UPF0759_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348:SF4">
        <property type="entry name" value="DUF72 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YECE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01904">
        <property type="entry name" value="DUF72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117396">
        <property type="entry name" value="TM1631-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="26861" checksum="99C7D18D67FD2A43" modified="2001-10-01" version="1">MKIFVGTSGWTYDWNDDDTLEWYVKNSGLNAVEVNMSFYRYPTRKQVERWSKFKEIRWIVKVNRRITHMKRLKDFQSWKEFQQIVDSLNPDFYLFQLPPTFKRNSENEKRVFQFAEVLKDKMAVEFRDIQWYLKPLNVTCVIVSIDSPIGTYVIKNNDYIYLRLHGRDVWYTYDYSEKELKELADKIISLNPKYVYVFFNNNHWMLDNGRYMLKILTEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZU7</accession>
    <name>Q97ZU7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Elongator complex protein 3</fullName>
            <ecNumber evidence="15">2.3.1.311</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">tRNA uridine(34) acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">SSO0480</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="17">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELP3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.311" evidence="15"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90193">
        <property type="entry name" value="B90193"/>
    </dbReference>
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        <property type="protein sequence ID" value="AAK40801"/>
        <property type="gene ID" value="SSO0480"/>
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    <dbReference type="PATRIC" id="fig|273057.12.peg.474"/>
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        <property type="component" value="Chromosome"/>
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        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
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    </dbReference>
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        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11 17" id="KW-0408">Iron</keyword>
    <keyword evidence="12 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="19">
        <location>
            <begin position="11"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="18">
        <location>
            <begin position="306"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
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            <position position="31"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
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        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030769"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00044771"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAK40801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="473" mass="54708" checksum="3DD75FAA5D013670" modified="2001-10-01" version="1">MQVIRKPTRMLSGVTIVSIMTHPHSCPHGKCVFCPGGVDVDTPQSYYGREPTLMRAIENDYDPFHQVQSRLRQYVENGHTPSKVELIIMGGTFLSLPIDYQDWFVTYALEAMNRFPSYDKPSFVYLEDAQVKNEKAEVRCVGMTIETKPDWAKEWHADQMLRLGATKVELGVQTVYDDILKFTNRGHTVKDSIESTRILKDSGFKVVYHVMLGLPKSDPDKDLEAFKTIFSDPNFRPDMLKIYPTLVVETAPLANLWRRGLYKPYGTETLIELISEMYRYIPKWVRVMRIQRDIPANVILDGNKKGNLRELVEKKVLEKGIKINEIRFREVGMMWQHRGLLPDHTKIHLYKEVYEASEGTEIFLSFEDEKEILIGYLRLRITSNKAHRKEIDGKTAIVRELHVYGIEVPIGSWDELGFQHRGYGSKLLSEAEKIAREEFDMRKISVLSGIGAREYYAKRGYVKEGPYMSKQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-04-02" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZV5</accession>
    <name>Q97ZV5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translation elongation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="B90192"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922987.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZV5"/>
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    <dbReference type="STRING" id="273057.SSO0470"/>
    <dbReference type="PaxDb" id="273057-SSO0470"/>
    <dbReference type="DNASU" id="1455603"/>
    <dbReference type="EnsemblBacteria" id="AAK40793">
        <property type="protein sequence ID" value="AAK40793"/>
        <property type="gene ID" value="SSO0470"/>
    </dbReference>
    <dbReference type="GeneID" id="15298034"/>
    <dbReference type="KEGG" id="sso:SSO0470"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.466"/>
    <dbReference type="eggNOG" id="arCOG01564">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_913990_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZV5"/>
    <dbReference type="PhylomeDB" id="Q97ZV5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000154">
        <property type="entry name" value="Selenocysteine-specific translation elongation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF11">
        <property type="entry name" value="SELENOCYSTEINE-SPECIFIC ELONGATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="33641" checksum="53829CF4539BDE7A" modified="2001-10-01" version="1">MYYGSIITVLSSDKNKLTGIAEKLGKLHETAKTKIYYRKKGEYIRSILLTTEYPEKIIDLAEALSLSSTAILYIPETLTWMDGELALLIDSLNTHNKIVISNLDQGKINNILGSLSSFTKFDLYNDIPDLSETEEEDKGIVYVDRVFTVKGVGTVVTGFSFTNVEVHEKLVALPYNKEVEIKSIQVLDEDQKSVSTGVRIGFALKNVKEEEIEDLLYLVKPNVKVVKEIEGQITNYKWSTINQGQNHIIVKGHGIAANIKVDNQKAKIMSSVPIPIVDNRILVLNVNVRQGKPRVAGYISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZW7</accession>
    <name>Q97ZW7_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lysine transporter LysE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO0455</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90190">
        <property type="entry name" value="F90190"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988702.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZW7"/>
    <dbReference type="STRING" id="273057.SSO0455"/>
    <dbReference type="PaxDb" id="273057-SSO0455"/>
    <dbReference type="EnsemblBacteria" id="AAK40781">
        <property type="protein sequence ID" value="AAK40781"/>
        <property type="gene ID" value="SSO0455"/>
    </dbReference>
    <dbReference type="GeneID" id="1455591"/>
    <dbReference type="KEGG" id="sso:SSO0455"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.450"/>
    <dbReference type="eggNOG" id="arCOG01947">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087840_1_2_2"/>
    <dbReference type="InParanoid" id="Q97ZW7"/>
    <dbReference type="PhylomeDB" id="Q97ZW7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38825">
        <property type="entry name" value="LYSINE EXPORTER PROTEIN (LYSE/YGGA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38825:SF2">
        <property type="entry name" value="LYSINE TRANSPORTER LYSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK40781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="20439" checksum="DC8879477C7935D2" modified="2001-10-01" version="1">MLLTYLGLGIILGLSMAAPPGPVNAMIANESMKSWLHGSSIGAGAMTADLIFFIIVYFIQGYIPTPIRNALYIIGGIFMLYLSYLTIRAKMPSKSIKGNYFIGLSMGLTNPYQISWWITVGISIIRSLSILIIPGFFVGILIWIIAFPKAVNMLGSKYVKYVKVISSVILVIFGVYLLYEGILNVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-28" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q980F9</accession>
    <name>RL39_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein eL39</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">50S ribosomal protein L39e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl39e</name>
        <name type="ordered locus">SSO5672</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40683" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40683.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90178">
        <property type="entry name" value="D90178"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990648.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980F9"/>
    <dbReference type="SMR" id="Q980F9"/>
    <dbReference type="FunCoup" id="Q980F9">
        <property type="interactions" value="116"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO5672"/>
    <dbReference type="PaxDb" id="273057-SSO5672"/>
    <dbReference type="EnsemblBacteria" id="AAK40683">
        <property type="protein sequence ID" value="AAK40683"/>
        <property type="gene ID" value="SSO5672"/>
    </dbReference>
    <dbReference type="GeneID" id="15298160"/>
    <dbReference type="KEGG" id="sso:SSO5672"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.344"/>
    <dbReference type="eggNOG" id="arCOG04177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181948_4_0_2"/>
    <dbReference type="InParanoid" id="Q980F9"/>
    <dbReference type="PhylomeDB" id="Q980F9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1620.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein-like L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.10">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00629">
        <property type="entry name" value="Ribosomal_eL39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000077">
        <property type="entry name" value="Ribosomal_eL39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020083">
        <property type="entry name" value="Ribosomal_eL39_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023626">
        <property type="entry name" value="Ribosomal_eL39_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002316">
        <property type="entry name" value="PRK01242.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19970:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19970">
        <property type="entry name" value="RIBOSOMAL PROTEIN L39E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00832">
        <property type="entry name" value="Ribosomal_L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48662">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00051">
        <property type="entry name" value="RIBOSOMAL_L39E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000127062" description="Large ribosomal subunit protein eL39">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="51" mass="6127" checksum="C2A4B1FCACEDEEBF" modified="2003-03-28" version="2">MSRNKPVAKKFRLAKALKANSPIPIWIVLKTRGRVRYNPLRRNWRRNDLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>Q980I0</accession>
    <name>Q980I0_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO0315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90174">
        <property type="entry name" value="D90174"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922937.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980I0"/>
    <dbReference type="STRING" id="273057.SSO0315"/>
    <dbReference type="PaxDb" id="273057-SSO0315"/>
    <dbReference type="EnsemblBacteria" id="AAK40651">
        <property type="protein sequence ID" value="AAK40651"/>
        <property type="gene ID" value="SSO0315"/>
    </dbReference>
    <dbReference type="GeneID" id="1455461"/>
    <dbReference type="KEGG" id="sso:SSO0315"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.309"/>
    <dbReference type="eggNOG" id="arCOG07278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_441893_0_0_2"/>
    <dbReference type="InParanoid" id="Q980I0"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="640" mass="73391" checksum="77556028ABEDF3BE" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-16" modified="2026-01-28" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q980I4</accession>
    <name>AROD_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-dehydroquinate dehydratase</fullName>
            <shortName evidence="1">3-dehydroquinase</shortName>
            <ecNumber evidence="1">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Type I DHQase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Type I dehydroquinase</fullName>
            <shortName evidence="1">DHQ1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroD</name>
        <name type="ordered locus">SSO0311</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="PIR" id="H90173">
        <property type="entry name" value="H90173"/>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
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    <dbReference type="AlphaFoldDB" id="Q980I4"/>
    <dbReference type="SMR" id="Q980I4"/>
    <dbReference type="FunCoup" id="Q980I4">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0311"/>
    <dbReference type="PaxDb" id="273057-SSO0311"/>
    <dbReference type="EnsemblBacteria" id="AAK40647">
        <property type="protein sequence ID" value="AAK40647"/>
        <property type="gene ID" value="SSO0311"/>
    </dbReference>
    <dbReference type="GeneID" id="1455457"/>
    <dbReference type="KEGG" id="sso:SSO0311"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.303"/>
    <dbReference type="eggNOG" id="arCOG02097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064444_2_0_2"/>
    <dbReference type="InParanoid" id="Q980I4"/>
    <dbReference type="PhylomeDB" id="Q980I4"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00086"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046279">
        <property type="term" value="P:3,4-dihydroxybenzoate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00502">
        <property type="entry name" value="DHQase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00214">
        <property type="entry name" value="AroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
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    <dbReference type="InterPro" id="IPR001381">
        <property type="entry name" value="DHquinase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050146">
        <property type="entry name" value="Type-I_3-dehydroquinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010091">
        <property type="entry name" value="PRK13576.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43699">
        <property type="entry name" value="3-DEHYDROQUINATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43699:SF1">
        <property type="entry name" value="3-DEHYDROQUINATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01487">
        <property type="entry name" value="DHquinase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000138838" description="3-dehydroquinate dehydratase">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="30"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00214"/>
        </source>
    </evidence>
    <sequence length="220" mass="25651" checksum="61BE4B4573C3750B" modified="2001-10-01" version="1">MRPLIVASLPIKKIEDLKLIENFLDADLIELRLDYLREREVSLISDYYEFLDKYKKKLIVTLRDKGEGGINQLADELKIKILNELYERQYLYDIEVSFLQKYDIPYDNRIVSVHYFNYLPTLEKIKEIVSKFSEKAFSVKIAVPSLKGYKEVLLPLLEYENVTVIPMSNNSLERIAVGLLGSKLVYSYAIEPLAQGQLYYKKVIQIFNYINDITTSSLVT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2013-12-11" modified="2026-01-28" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q980J9</accession>
    <name>CSL4_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Exosome complex component Csl4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">csl4</name>
        <name type="ordered locus">SSO0292</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="EMBO Rep." volume="4" first="889" last="893">
            <title>An exosome-like complex in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Evguenieva-Hackenberg E."/>
                <person name="Walter P."/>
                <person name="Hochleitner E."/>
                <person name="Lottspeich F."/>
                <person name="Klug G."/>
            </authorList>
            <dbReference type="PubMed" id="12947419"/>
            <dbReference type="DOI" id="10.1038/sj.embor.embor929"/>
        </citation>
        <scope>INTERACTION WITH EXOSOME</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2006" name="Mol. Microbiol." volume="62" first="1076" last="1089">
            <title>Characterization of native and reconstituted exosome complexes from the hyperthermophilic archaeon Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Walter P."/>
                <person name="Klein F."/>
                <person name="Lorentzen E."/>
                <person name="Ilchmann A."/>
                <person name="Klug G."/>
                <person name="Evguenieva-Hackenberg E."/>
            </authorList>
            <dbReference type="PubMed" id="17078816"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.2006.05393.x"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH EXOSOME</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2008" name="Biochemistry" volume="47" first="13158" last="13168">
            <title>Rrp4 and Csl4 are needed for efficient degradation but not for polyadenylation of synthetic and natural RNA by the archaeal exosome.</title>
            <authorList>
                <person name="Evguenieva-Hackenberg E."/>
                <person name="Roppelt V."/>
                <person name="Finsterseifer P."/>
                <person name="Klug G."/>
            </authorList>
            <dbReference type="PubMed" id="19053279"/>
            <dbReference type="DOI" id="10.1021/bi8012214"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2010" name="FEBS Lett." volume="584" first="2931" last="2936">
            <title>The evolutionarily conserved subunits Rrp4 and Csl4 confer different substrate specificities to the archaeal exosome.</title>
            <authorList>
                <person name="Roppelt V."/>
                <person name="Klug G."/>
                <person name="Evguenieva-Hackenberg E."/>
            </authorList>
            <dbReference type="PubMed" id="20488184"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2010.05.014"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2012" name="Biochimie" volume="94" first="1578" last="1587">
            <title>Heterogeneous complexes of the RNA exosome in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Witharana C."/>
                <person name="Roppelt V."/>
                <person name="Lochnit G."/>
                <person name="Klug G."/>
                <person name="Evguenieva-Hackenberg E."/>
            </authorList>
            <dbReference type="PubMed" id="22503705"/>
            <dbReference type="DOI" id="10.1016/j.biochi.2012.03.026"/>
        </citation>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2013" name="RNA Biol." volume="10" first="415" last="424">
            <title>The archaeal DnaG protein needs Csl4 for binding to the exosome and enhances its interaction with adenine-rich RNAs.</title>
            <authorList>
                <person name="Hou L."/>
                <person name="Klug G."/>
                <person name="Evguenieva-Hackenberg E."/>
            </authorList>
            <dbReference type="PubMed" id="23324612"/>
        </citation>
        <scope>INTERACTION WITH DNAG</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3 4 5">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Increases the RNA binding and the efficiency of RNA degradation. Helpful for the interaction of the exosome with A-poor RNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3 6 7">Component of the archaeal exosome complex. Forms a trimer of Rrp4 and/or Csl4 subunits. The trimer associates with a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. Interacts with DnaG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90172">
        <property type="entry name" value="A90172"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990582.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980J9"/>
    <dbReference type="SMR" id="Q980J9"/>
    <dbReference type="STRING" id="273057.SSO0292"/>
    <dbReference type="PaxDb" id="273057-SSO0292"/>
    <dbReference type="EnsemblBacteria" id="AAK40632">
        <property type="protein sequence ID" value="AAK40632"/>
        <property type="gene ID" value="SSO0292"/>
    </dbReference>
    <dbReference type="GeneID" id="1455442"/>
    <dbReference type="KEGG" id="sso:SSO0292"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.287"/>
    <dbReference type="eggNOG" id="arCOG00676">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067135_1_1_2"/>
    <dbReference type="InParanoid" id="Q980J9"/>
    <dbReference type="PhylomeDB" id="Q980J9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00975">
        <property type="entry name" value="Exosome_Csl4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039771">
        <property type="entry name" value="Csl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025721">
        <property type="entry name" value="Exosome_cplx_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030850">
        <property type="entry name" value="Exosome_Csl4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF034126">
        <property type="entry name" value="PRK09521.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE CSL4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT CSL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14382">
        <property type="entry name" value="ECR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0271">Exosome</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000424721" description="Exosome complex component Csl4">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="66"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12947419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="17078816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="19053279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="20488184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="22503705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="7">
        <source>
            <dbReference type="PubMed" id="23324612"/>
        </source>
    </evidence>
    <sequence length="189" mass="21004" checksum="A0D53D88B49E6FDB" modified="2001-10-01" version="1">MRTQGELTLPGEELAVIEEFMTGDGTYEQNGIVRAAVVGKIFYDMLNRKSNVLGFKKIIFQHLKKAKYVIGIVNSIKEDSALVSIVGIEERGLASPISAYLHISQISNKKINNVTDAIKIGDVIKAKLLSYTFPLALTIKMKDLGVIYARCSRCGYLLIKQDENNLKCQRCGNIEQRKIGSYMVKKGGN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-03-20" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q980R9</accession>
    <name>NAT_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">N-alpha-acetyltransferase</fullName>
            <shortName evidence="6">NAT</shortName>
            <ecNumber evidence="2 3 4">2.3.1.255</ecNumber>
            <ecNumber evidence="2 3">2.3.1.258</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Amino-terminal acetyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">N-terminal acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ard1</name>
        <name type="ordered locus">SSO0209</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2007" name="Mol. Microbiol." volume="64" first="1540" last="1548">
            <title>An acetylase with relaxed specificity catalyses protein N-terminal acetylation in Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Mackay D.T."/>
                <person name="Botting C.H."/>
                <person name="Taylor G.L."/>
                <person name="White M.F."/>
            </authorList>
            <dbReference type="PubMed" id="17511810"/>
            <dbReference type="DOI" id="10.1111/j.1365-2958.2007.05752.x"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="J. Struct. Funct. Genomics" volume="11" first="167" last="180">
            <title>The Scottish Structural Proteomics Facility: targets, methods and outputs.</title>
            <authorList>
                <person name="Oke M."/>
                <person name="Carter L.G."/>
                <person name="Johnson K.A."/>
                <person name="Liu H."/>
                <person name="McMahon S.A."/>
                <person name="Yan X."/>
                <person name="Kerou M."/>
                <person name="Weikart N.D."/>
                <person name="Kadi N."/>
                <person name="Sheikh M.A."/>
                <person name="Schmelz S."/>
                <person name="Dorward M."/>
                <person name="Zawadzki M."/>
                <person name="Cozens C."/>
                <person name="Falconer H."/>
                <person name="Powers H."/>
                <person name="Overton I.M."/>
                <person name="van Niekerk C.A."/>
                <person name="Peng X."/>
                <person name="Patel P."/>
                <person name="Garrett R.A."/>
                <person name="Prangishvili D."/>
                <person name="Botting C.H."/>
                <person name="Coote P.J."/>
                <person name="Dryden D.T."/>
                <person name="Barton G.J."/>
                <person name="Schwarz-Linek U."/>
                <person name="Challis G.L."/>
                <person name="Taylor G.L."/>
                <person name="White M.F."/>
                <person name="Naismith J.H."/>
            </authorList>
            <dbReference type="PubMed" id="20419351"/>
            <dbReference type="DOI" id="10.1007/s10969-010-9090-y"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2013" name="Proc. Natl. Acad. Sci. U.S.A." volume="110" first="14652" last="14657">
            <title>Implications for the evolution of eukaryotic amino-terminal acetyltransferase (NAT) enzymes from the structure of an archaeal ortholog.</title>
            <authorList>
                <person name="Liszczak G."/>
                <person name="Marmorstein R."/>
            </authorList>
            <dbReference type="PubMed" id="23959863"/>
            <dbReference type="DOI" id="10.1073/pnas.1310365110"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.98 ANGSTROMS) IN COMPLEX WITH ZINC ION AND ACETYL-COA</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>MUTAGENESIS OF LEU-33; PRO-34; GLU-35; TYR-37; HIS-88; TYR-125; GLU-127; ARG-129 AND TYR-154</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2015" name="Sci. Rep." volume="5" first="8673" last="8673">
            <title>Structural basis for substrate-specific acetylation of Nalpha-acetyltransferase Ard1 from Sulfolobus solfataricus.</title>
            <authorList>
                <person name="Chang Y.Y."/>
                <person name="Hsu C.H."/>
            </authorList>
            <dbReference type="PubMed" id="25728374"/>
            <dbReference type="DOI" id="10.1038/srep08673"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOGS</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>MUTAGENESIS OF GLU-35; ARG-100; THR-105 AND ASN-132</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2016" name="ChemBioChem" volume="17" first="214" last="217">
            <title>Multiple conformations of the loop region confers heat-resistance on ssArd1, a Thermophilic NatA.</title>
            <authorList>
                <person name="Chang Y.Y."/>
                <person name="Hsu C.H."/>
            </authorList>
            <dbReference type="PubMed" id="26593285"/>
            <dbReference type="DOI" id="10.1002/cbic.201500568"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.49 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG</scope>
        <scope>MUTAGENESIS OF SER-75 AND SER-82</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3 4">Displays alpha (N-terminal) acetyltransferase activity. Catalyzes the covalent attachment of an acetyl moiety from acetyl-CoA to the free alpha-amino group at the N-terminus of a protein (PubMed:17511810, PubMed:23959863, PubMed:25728374). NAT is able to acetylate the alpha-amino group of methionine, alanine and serine N-terminal residue substrates, however it has a preference for Ser-N-terminal substrates (PubMed:17511810, PubMed:23959863, PubMed:25728374).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 4">
            <text>N-terminal L-alanyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-alanyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50500"/>
            <dbReference type="Rhea" id="RHEA-COMP:12701"/>
            <dbReference type="Rhea" id="RHEA-COMP:12702"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64718"/>
            <dbReference type="ChEBI" id="CHEBI:83683"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3 4">
            <text>N-terminal L-seryl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50504"/>
            <dbReference type="Rhea" id="RHEA-COMP:12703"/>
            <dbReference type="Rhea" id="RHEA-COMP:12704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>N-terminal L-methionyl-L-leucyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-leucyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50520"/>
            <dbReference type="Rhea" id="RHEA-COMP:12711"/>
            <dbReference type="Rhea" id="RHEA-COMP:12712"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133377"/>
            <dbReference type="ChEBI" id="CHEBI:133378"/>
            <dbReference type="EC" id="2.3.1.258"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>N-terminal L-methionyl-L-glutamyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-glutamyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50488"/>
            <dbReference type="Rhea" id="RHEA-COMP:12696"/>
            <dbReference type="Rhea" id="RHEA-COMP:12697"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133359"/>
            <dbReference type="ChEBI" id="CHEBI:133360"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">54 uM for Ser-N-terminal peptide</KM>
            <KM evidence="4">67.17 uM for acetyl-CoA (at 65 degrees Celsius with Ser-N-terminal peptide (Alba))</KM>
            <KM evidence="3">400 uM for Met-N-terminal peptide</KM>
            <text evidence="3 4">kcat is 33.57 min(-1) for acetyltransferase activity with acetyl-CoA (at 65 degrees Celsius with Ser-N-terminal peptide (Alba)) (PubMed:25728374). kcat is 3.3 min(-1) for acetyltransferase activity with Ser-N-terminal peptide (PubMed:23959863). kcat is 0.73 min(-1) for acetyltransferase activity with Met-N-terminal peptide (PubMed:23959863).</text>
        </kinetics>
        <temperatureDependence>
            <text evidence="5">Thermostable.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2 3">NAT does not require a binding partner for activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="7">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40554" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.255" evidence="2 3 4"/>
    <dbReference type="EC" id="2.3.1.258" evidence="2 3"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40554.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90162">
        <property type="entry name" value="C90162"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_173645072.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="2X7B">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.95 A"/>
        <property type="chains" value="A=1-167"/>
    </dbReference>
    <dbReference type="PDB" id="4LX9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.98 A"/>
        <property type="chains" value="A=1-167"/>
    </dbReference>
    <dbReference type="PDB" id="4R3K">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.13 A"/>
        <property type="chains" value="A=1-167"/>
    </dbReference>
    <dbReference type="PDB" id="4R3L">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.84 A"/>
        <property type="chains" value="A=1-167"/>
    </dbReference>
    <dbReference type="PDB" id="5C88">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.49 A"/>
        <property type="chains" value="A/B=1-167"/>
    </dbReference>
    <dbReference type="PDB" id="6AG4">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.26 A"/>
        <property type="chains" value="A=1-167"/>
    </dbReference>
    <dbReference type="PDB" id="6AG5">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.32 A"/>
        <property type="chains" value="A=1-167"/>
    </dbReference>
    <dbReference type="PDBsum" id="2X7B"/>
    <dbReference type="PDBsum" id="4LX9"/>
    <dbReference type="PDBsum" id="4R3K"/>
    <dbReference type="PDBsum" id="4R3L"/>
    <dbReference type="PDBsum" id="5C88"/>
    <dbReference type="PDBsum" id="6AG4"/>
    <dbReference type="PDBsum" id="6AG5"/>
    <dbReference type="AlphaFoldDB" id="Q980R9"/>
    <dbReference type="SMR" id="Q980R9"/>
    <dbReference type="FunCoup" id="Q980R9">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0209"/>
    <dbReference type="PaxDb" id="273057-SSO0209"/>
    <dbReference type="DNASU" id="1455364"/>
    <dbReference type="EnsemblBacteria" id="AAK40554">
        <property type="protein sequence ID" value="AAK40554"/>
        <property type="gene ID" value="SSO0209"/>
    </dbReference>
    <dbReference type="GeneID" id="1455364"/>
    <dbReference type="KEGG" id="sso:SSO0209"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.208"/>
    <dbReference type="eggNOG" id="arCOG00833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_23_0_2"/>
    <dbReference type="InParanoid" id="Q980R9"/>
    <dbReference type="PhylomeDB" id="Q980R9"/>
    <dbReference type="BRENDA" id="2.3.1.255">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q980R9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120518">
        <property type="term" value="F:protein N-terminal-methionine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008999">
        <property type="term" value="F:protein-N-terminal-alanine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000200">
        <property type="entry name" value="N-alpha-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091:SF4">
        <property type="entry name" value="N-TERMINAL AMINO-ACID N(ALPHA)-ACETYLTRANSFERASE NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000281644" description="N-alpha-acetyltransferase">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="12"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 12">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 10">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10 11">
        <location>
            <begin position="92"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10 11">
        <location>
            <begin position="100"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 10">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10 11">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10 11">
        <location>
            <begin position="139"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 12">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Plays an important role in substrate specificity" evidence="4">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="site" description="Plays an important role in modulating multiple conformations of loop regions and contributes to protein thermostability" evidence="5">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="site" description="Plays an important role in modulating multiple conformations of loop regions and contributes to protein thermostability" evidence="5">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="20- and 2-fold decrease of the catalytic efficiency and affinity for Ser-N-terminal peptide. 11-fold decrease of the catalytic efficiency for Met-N-terminal peptide, but almost same affinity compared to the wild-type." evidence="3">
        <original>L</original>
        <variation>A</variation>
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="20-fold decrease of the catalytic efficiency for Ser-N-terminal peptide, but almost same affinity compared to the wild-type. 18-fold decrease of the catalytic efficiency for Met-N-terminal peptide, but almost same affinity compared to the wild-type." evidence="3 4">
        <original>P</original>
        <variation>A</variation>
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Slight increase of the catalytic efficiency for Ser-N-terminal peptide, but 4-fold decrease of the affinity compared to the wild-type. 6-fold increase of the catalytic efficiency for Met-N-terminal peptide and slight decrease of the affinity compared to the wild-type." evidence="3 4">
        <original>E</original>
        <variation>A</variation>
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Strong decrease of the acetyltransferase activity with Ser-N-terminal peptide such as Alba. 2-fold increase of acetyltransferase activity for Ala-N-terminal peptide such as Hjc compared to the wild-type." evidence="4">
        <original>E</original>
        <variation>F</variation>
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of acetyltransferase activity for Ser and Met-N-terminal peptide; when associated with Gln-127." evidence="3">
        <original>E</original>
        <variation>Q</variation>
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Alters the N-terminal substrate specificity and allows large N-terminal end residue of the substrate to be accommodated in a substrate-binding pocket. 4-fold increase of the acetyltransferase activity with Met-N-terminal peptide such as SSB compared to the wild-type. 2-fold increase of acetyltransferase activity with Ala-N-terminal peptide such as Hjc." evidence="4">
        <original>E</original>
        <variation>V</variation>
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Low acetyltransferase activity with Ala-, Met- and Ser-N-terminal peptide." evidence="4">
        <original>E</original>
        <variation>W</variation>
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="34-fold decrease of the catalytic efficiency for Ser-N-terminal peptide and slight decrease of the affinity compared to the wild-type. Loss of acetyltransferase activity for Met-N-terminal peptide." evidence="3">
        <original>Y</original>
        <variation>A</variation>
        <location>
            <position position="37"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Same catalytic efficiency and slight decrease of the affinity for Ser-N-terminal peptide compared to the wild-type. 3-fold decrease of the catalytic efficiency and 1.3-fold increase of the affinity for Met-N-terminal peptide compared to the wild-type." evidence="3">
        <original>Y</original>
        <variation>F</variation>
        <location>
            <position position="37"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Has a melting temperature about 3 degrees Celsius lower than that of the wild-type." evidence="5">
        <original>S</original>
        <variation>A</variation>
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Has a melting temperature about 3 degrees Celsius lower than that of the wild-type." evidence="5">
        <original>S</original>
        <variation>A</variation>
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="2.5- and 1.5-fold decrease of the catalytic efficiency and affinity for Ser-N-terminal peptide compared to the wild-type, respectively. Loss of acetyltransferase activity for Met-N-terminal peptide." evidence="3">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="2.5-fold decrease of the catalytic efficiency for Ser-N-terminal peptide, but almost same affinity compared to the wild-type. Loss of acetyltransferase activity for Met-N-terminal peptide." evidence="3">
        <original>H</original>
        <variation>F</variation>
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="2.5-fold decrease of the catalytic efficiency for Ser-N-terminal peptide, but 1.5-fold increase of the affinity compared to the wild-type. Loss of acetyltransferase activity for Met-N-terminal peptide." evidence="3">
        <original>H</original>
        <variation>Q</variation>
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="7-fold decrease of the affinity, with no significant difference in the catalytic efficiency. Same fold compared to the wild-type." evidence="4">
        <original>R</original>
        <variation>A</variation>
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="3-fold decrease of the affinity, with no significant difference in the catalytic efficiency. Same fold compared to the wild-type." evidence="4">
        <original>T</original>
        <variation>A</variation>
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Same catalytic efficiency and 1.7-fold decrease of the affinity for Ser-N-terminal peptide compared to the wild-type. 1.5- and 2.5-fold decrease of the catalytic efficiency and affinity for Met-N-terminal peptide compared to the wild-type, respectively." evidence="3">
        <original>Y</original>
        <variation>A</variation>
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Same catalytic efficiency and slight decrease of the affinity for Ser-N-terminal peptide compared to the wild-type. Loss of acetyltransferase activity for Met-N-terminal peptide." evidence="3">
        <original>E</original>
        <variation>A</variation>
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="1.3-fold decrease of the catalytic efficiency for Ser-N-terminal peptide, but almost same affinity compared to the wild-type. 1.7-fold decrease of the catalytic efficiency and 1.3-fold increase of the affinity for Met-N-terminal peptide compared to the wild-type." evidence="3">
        <original>E</original>
        <variation>H</variation>
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="2.3-fold decrease of the catalytic efficiency and 1.3-fold increase of the affinity for Ser-N-terminal peptide compared to the wild-type. 5-fold decrease of the catalytic efficiency and slight decrease of the affinity for Met-N-terminal peptide compared to the wild-type. Loss of acetyltransferase activity for Ser and Met-N-terminal peptide; when associated with Gln-35." evidence="3">
        <original>E</original>
        <variation>Q</variation>
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Slight decrease of the catalytic efficiency and of the affinity for Ser-N-terminal peptide compared to teh wild-type. 2.5-fold increase of the catalytic efficiency and almost the same affinity for Met-N-terminal peptide compared to the wild-type." evidence="3">
        <original>R</original>
        <variation>A</variation>
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="4.5-fold decrease of the affinity, with no significant difference in the catalytic efficiency. Same fold compared to the wild-type." evidence="4">
        <original>N</original>
        <variation>A</variation>
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="1.3-fold decrease of the catalytic efficiency for Ser-N-terminal peptide, but same affinity compared to the wild-type. 6.5-fold decrease of the catalytic efficiency for Met-N-terminal peptide, but same affinity compared to the wild-type." evidence="3">
        <original>Y</original>
        <variation>A</variation>
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Almost same catalytic efficiency for Ser-N-terminal peptide and slight decrease of the affinity compared to the wild-type. 5-fold decrease of the catalytic efficiency for Met-N-terminal peptide and 1.5-fold decrease of the affinity compared to the wild-type." evidence="3">
        <original>Y</original>
        <variation>F</variation>
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="strand" evidence="14">
        <location>
            <begin position="13"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="helix" evidence="14">
        <location>
            <begin position="19"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="helix" evidence="14">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="helix" evidence="14">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="helix" evidence="14">
        <location>
            <begin position="50"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="strand" evidence="14">
        <location>
            <begin position="54"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="strand" evidence="14">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="76"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="strand" evidence="14">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="helix" evidence="14">
        <location>
            <begin position="96"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="strand" evidence="14">
        <location>
            <begin position="100"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="helix" evidence="14">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="strand" evidence="14">
        <location>
            <begin position="122"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="helix" evidence="14">
        <location>
            <begin position="133"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="strand" evidence="14">
        <location>
            <begin position="145"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="strand" evidence="15">
        <location>
            <begin position="154"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="strand" evidence="14">
        <location>
            <begin position="160"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="17511810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="23959863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="25728374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="26593285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="6">
        <source>
            <dbReference type="PubMed" id="17511810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <evidence type="ECO:0000305" key="8">
        <source>
            <dbReference type="PubMed" id="26593285"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="2X7B"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="10">
        <source>
            <dbReference type="PDB" id="4LX9"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="11">
        <source>
            <dbReference type="PDB" id="4R3K"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="12">
        <source>
            <dbReference type="PDB" id="4R3L"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="13">
        <source>
            <dbReference type="PDB" id="4R3K"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="14">
        <source>
            <dbReference type="PDB" id="4R3L"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="15">
        <source>
            <dbReference type="PDB" id="5C88"/>
        </source>
    </evidence>
    <sequence length="167" mass="19448" checksum="410D4CA4F7CEA60E" modified="2007-03-20" version="2">MELAEKDKGRDFTLRNARMDDIDQIIKINRLTLPENYPYYFFVEHLKEYGLAFFVAIVDNSVVGYIMPRIEWGFSNIKQLPSLVRKGHVVSIAVLEEYRRKGIATTLLEASMKSMKNDYNAEEIYLEVRVSNYPAIALYEKLNFKKVKVLKGYYADGEDAYLMARPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q980X4</accession>
    <name>Q980X4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">D-arabino 3-hexulose 6-phosphate formaldehyde lyase (HpS-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hpS-1</name>
        <name evidence="3" type="ordered locus">SSO0151</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIS family. PHI subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90155">
        <property type="entry name" value="C90155"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990377.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980X4"/>
    <dbReference type="FunCoup" id="Q980X4">
        <property type="interactions" value="189"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0151"/>
    <dbReference type="PaxDb" id="273057-SSO0151"/>
    <dbReference type="EnsemblBacteria" id="AAK40498">
        <property type="protein sequence ID" value="AAK40498"/>
        <property type="gene ID" value="SSO0151"/>
    </dbReference>
    <dbReference type="GeneID" id="1453325"/>
    <dbReference type="KEGG" id="sso:SSO0151"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.149"/>
    <dbReference type="eggNOG" id="arCOG00068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094236_1_1_2"/>
    <dbReference type="InParanoid" id="Q980X4"/>
    <dbReference type="PhylomeDB" id="Q980X4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05005">
        <property type="entry name" value="SIS_PHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017552">
        <property type="entry name" value="PHI/rmpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03127">
        <property type="entry name" value="RuMP_HxlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43443">
        <property type="entry name" value="3-HEXULOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43443:SF1">
        <property type="entry name" value="3-HEXULOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="2">
        <location>
            <begin position="41"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="209" mass="22981" checksum="6DE48593FD6AE114" modified="2001-10-01" version="1">MSSSLDDYPLSLKTMYDIAEFILRAAKAIKPEQTIKMVNELENFYKNNRNGKVLVMGAGRSGLVGRAFAMRLLHLGFNSYVLGETIVPAIGKNDIVVAISGSGRTKLILTAAEAAKEAGAKLISITSYFDSPLAKISDVVIEIPGRTKYSKNEDYFARQILGITEPLAPLGTLFEDTTQIFLDGIVAELMIRLKKTEEDLRLIHANIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Y9</accession>
    <name>Q980Y9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cobalamin biosynthesis protein G (CbiG)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbiG</name>
        <name evidence="1" type="ordered locus">SSO0129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90153">
        <property type="entry name" value="D90153"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988956.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Y9"/>
    <dbReference type="FunCoup" id="Q980Y9">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0129"/>
    <dbReference type="PaxDb" id="273057-SSO0129"/>
    <dbReference type="EnsemblBacteria" id="AAK40483">
        <property type="protein sequence ID" value="AAK40483"/>
        <property type="gene ID" value="SSO0129"/>
    </dbReference>
    <dbReference type="GeneID" id="1453310"/>
    <dbReference type="KEGG" id="sso:SSO0129"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.125"/>
    <dbReference type="eggNOG" id="arCOG00652">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1010546_0_0_2"/>
    <dbReference type="InParanoid" id="Q980Y9"/>
    <dbReference type="PhylomeDB" id="Q980Y9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052553">
        <property type="entry name" value="CbiG_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038029">
        <property type="entry name" value="GbiG_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37477">
        <property type="entry name" value="COBALT-PRECORRIN-5A HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37477:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-5A HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159672">
        <property type="entry name" value="CbiG N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="293" mass="33080" checksum="59570AB9F3E1C8E9" modified="2001-10-01" version="1">MYVSRILVNGVNKDLVKQIKGLFSELGYWIVYNDPDIIISVNSINNTIRKYFAKFHNKIIINVVPDGSSVIPLTKEHSGGSLISSFLADSLGANLVLTTSTGVKGLYSVEEFSWLNGFSIYGYDRKIVNSLNKKLVTEGKVNVYVDSYKGNYILYDGYSLVDHKTKADLIISENVDSMEDKSSNRLYLIPSGIYIGIRYLESTPLDVLVYSLRMTLKSLYILNDRIDYIVVPSSLKNDKKLHQITKYYYSNIIYGSDVDERSSCEALLKELKVKVLLKDVRRAFGVITCLGVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2026-01-28" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UWU0</accession>
    <name>HMGCS_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydroxymethylglutaryl-CoA synthase</fullName>
            <shortName evidence="1">HMG-CoA synthase</shortName>
            <shortName evidence="1">HMGCS</shortName>
            <ecNumber evidence="1">2.3.3.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0535</name>
        <name type="ORF">C22_016</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds that are building blocks of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.10"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:10189"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with acetoacetyl-CoA thiolase that catalyzes the precedent step in the pathway and with a DUF35 protein. The acetoacetyl-CoA thiolase/HMG-CoA synthase complex channels the intermediate via a fused CoA-binding site, which allows for efficient coupling of the endergonic thiolase reaction with the exergonic HMGCS reaction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Archaeal HMG-CoA synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.10" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90199">
        <property type="entry name" value="G90199"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991040.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UWU0"/>
    <dbReference type="SMR" id="Q9UWU0"/>
    <dbReference type="FunCoup" id="Q9UWU0">
        <property type="interactions" value="152"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0535"/>
    <dbReference type="PaxDb" id="273057-SSO0535"/>
    <dbReference type="EnsemblBacteria" id="AAK40854">
        <property type="protein sequence ID" value="AAK40854"/>
        <property type="gene ID" value="SSO0535"/>
    </dbReference>
    <dbReference type="GeneID" id="1454821"/>
    <dbReference type="KEGG" id="sso:SSO0535"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.533"/>
    <dbReference type="eggNOG" id="arCOG01767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039592_7_0_2"/>
    <dbReference type="InParanoid" id="Q9UWU0"/>
    <dbReference type="PhylomeDB" id="Q9UWU0"/>
    <dbReference type="UniPathway" id="UPA00058">
        <property type="reaction ID" value="UER00102"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000046">
        <property type="entry name" value="UPF0219 protein M1627_1703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01409">
        <property type="entry name" value="HMG_CoA_synth_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004656">
        <property type="entry name" value="HMG_CoA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00748">
        <property type="entry name" value="HMG_CoA_syn_Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003274">
        <property type="entry name" value="PRK04262.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000057625" description="Hydroxymethylglutaryl-CoA synthase">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="1">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>(3S)-3-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>(3S)-3-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>(3S)-3-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>(3S)-3-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>(3S)-3-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <note>ligand shared with acetoacetyl-CoA thiolase</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>(3S)-3-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>(3S)-3-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01409"/>
        </source>
    </evidence>
    <sequence length="350" mass="37923" checksum="A0840A9CCB57B0DF" modified="2000-05-01" version="1">MLSGILGWGAYIPRYRIKVEDIAKMWGYDEAVVKSLGLTEKSVPGHDEDSTTIAWESSINAIKRAQVDTSKIRLVLFGSESKVYAVKPTSTILIDALGINNYSATADMEFACRAASVGLRLASSFVLHNNDSYALVIGADTAQSNPGDVLELSSAAAGVAFVVGKTDEKHSAAVIEYSSSYTSDTPDFWRRDGTPYPVHGEGFTGEPAYFHHIISAVNDLLQNSGLKISDFDYFVFHQPNGKFPIQVAKKLGVPLEKVKQGLVSPYIGNPYNASALLGLAKVLDIAKPGERILVAPFGSGAGSDAFSILVSEGILAKQKLAKTVEYYINNKKLVSYAEYAKYTNKIKVYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-10-24" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UWU1</accession>
    <name>ARGDC_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>Arginine decarboxylase proenzyme</fullName>
            <shortName>ADC</shortName>
            <shortName>ArgDC</shortName>
            <ecNumber>4.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Pyruvoyl-dependent arginine decarboxylase</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Arginine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Arginine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="ordered locus">SSO0536</name>
        <name type="ORF">C22_015</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="J. Biol. Chem." volume="283" first="25829" last="25838">
            <title>Crenarchaeal arginine decarboxylase evolved from an S-adenosylmethionine decarboxylase enzyme.</title>
            <authorList>
                <person name="Giles T.N."/>
                <person name="Graham D.E."/>
            </authorList>
            <dbReference type="PubMed" id="18650422"/>
            <dbReference type="DOI" id="10.1074/jbc.M802674200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>SUBUNIT</scope>
        <scope>MASS SPECTROMETRY</scope>
        <scope>SELF-PROCESSING</scope>
        <scope>CLEAVAGE SITE</scope>
        <scope>PYRUVATE FORMATION AT SER-82</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically catalyzes the decarboxylation of L-arginine to agmatine. Is also able to decarboxylate L-canavanine, although less efficiently. Has no S-adenosylmethionine decarboxylase (AdoMetDC) activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-arginine + H(+) = agmatine + CO2</text>
            <dbReference type="Rhea" id="RHEA:17641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="EC" id="4.1.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="2">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Highly competitively inhibited by L-argininamide and L-arginine methyl ester. Also inhibited by alpha-difluoromethylarginine. Is not stimulated by potassium chloride as observed for other decarboxylases.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">150 uM for L-arginine</KM>
            <Vmax evidence="2">1.1 umol/min/mg enzyme</Vmax>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 6.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 80 degrees Celsius. Highly thermostable. Retains 80% activity after incubation at 90 degrees Celsius for 10 minutes.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Amine and polyamine biosynthesis; agmatine biosynthesis; agmatine from L-arginine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterooctamer of four alpha and four beta chains arranged as a tetramer of alpha/beta heterodimers.</text>
    </comment>
    <comment type="PTM">
        <text evidence="2">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.</text>
    </comment>
    <comment type="mass spectrometry" mass="6123.0" method="Electrospray" evidence="2">
        <molecule>Arginine decarboxylase alpha chain</molecule>
        <text>Pyruvoyl group-containing alpha subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text>A chimeric protein containing the beta subunit of SSO0536 and the alpha subunit of SSO0585 (SpeH) has ArgDC activity and no AdoMetDC activity, implicating residues responsible for substrate specificity in the beta subunit. But additional factors in the alpha subunit are required for efficient cleavage and turnover.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the prokaryotic AdoMetDC family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.19"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90199">
        <property type="entry name" value="H90199"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991039.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UWU1"/>
    <dbReference type="SMR" id="Q9UWU1"/>
    <dbReference type="FunCoup" id="Q9UWU1">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0536"/>
    <dbReference type="PaxDb" id="273057-SSO0536"/>
    <dbReference type="EnsemblBacteria" id="AAK40855">
        <property type="protein sequence ID" value="AAK40855"/>
        <property type="gene ID" value="SSO0536"/>
    </dbReference>
    <dbReference type="GeneID" id="1454822"/>
    <dbReference type="KEGG" id="sso:SSO0536"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.534"/>
    <dbReference type="eggNOG" id="arCOG00279">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125470_2_1_2"/>
    <dbReference type="InParanoid" id="Q9UWU1"/>
    <dbReference type="PhylomeDB" id="Q9UWU1"/>
    <dbReference type="BRENDA" id="4.1.1.19">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q9UWU1"/>
    <dbReference type="UniPathway" id="UPA00186">
        <property type="reaction ID" value="UER00284"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008792">
        <property type="term" value="F:arginine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006527">
        <property type="term" value="P:L-arginine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.90.10:FF:000005">
        <property type="entry name" value="Arginine decarboxylase proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.90.10">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00464">
        <property type="entry name" value="AdoMetDC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01298">
        <property type="entry name" value="ArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003826">
        <property type="entry name" value="AdoMetDC_fam_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027549">
        <property type="entry name" value="ArgDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017716">
        <property type="entry name" value="S-AdoMet_deCOase_pro-enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03330">
        <property type="entry name" value="SAM_DCase_Bsu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866:SF2">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02675">
        <property type="entry name" value="AdoMet_dc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_0000030149" description="Arginine decarboxylase beta chain">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000030150" description="Arginine decarboxylase alpha chain">
        <location>
            <begin position="82"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="1">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="1">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for catalytic activity" evidence="1">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autolysis">
        <location>
            <begin position="81"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="2">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18650422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="134" mass="15247" checksum="E88793740CBA829D" modified="2000-05-01" version="1" precursor="true">MSEREVLQKINSPEGKEDRIIGKHVFGNLYDIDAERLNDKEFLEKLVLEAVDIAHMKLVEIKAWSFGGKKGGVSVIALVEESHIALHTWNEYNYATLDVYTCGEDSDPQSAFAHIVNALNPKRYQMFYADRSSQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-01-24" modified="2026-01-28" version="143" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UWW8</accession>
    <name>AATB_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">A-type ATP synthase subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpB</name>
        <name type="ordered locus">SSO0564</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane. The B chain is a regulatory subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90203">
        <property type="entry name" value="A90203"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991076.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UWW8"/>
    <dbReference type="SMR" id="Q9UWW8"/>
    <dbReference type="FunCoup" id="Q9UWW8">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0564"/>
    <dbReference type="PaxDb" id="273057-SSO0564"/>
    <dbReference type="EnsemblBacteria" id="AAK40880">
        <property type="protein sequence ID" value="AAK40880"/>
        <property type="gene ID" value="SSO0564"/>
    </dbReference>
    <dbReference type="GeneID" id="1454847"/>
    <dbReference type="KEGG" id="sso:SSO0564"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.573"/>
    <dbReference type="eggNOG" id="arCOG00865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022916_0_0_2"/>
    <dbReference type="InParanoid" id="Q9UWW8"/>
    <dbReference type="PhylomeDB" id="Q9UWW8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005724">
        <property type="entry name" value="ATPase_A1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01041">
        <property type="entry name" value="ATP_syn_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF4">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000144667" description="A-type ATP synthase subunit B">
        <location>
            <begin position="1"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00310"/>
        </source>
    </evidence>
    <sequence length="463" mass="51090" checksum="3BCB147339C5C1EF" modified="2000-05-01" version="1">MLSVREFSNISMIKGPLVYVQGVTDASYNELVEIEMPNGERRRGLVIDSQMGIAIVQVFEGTTGVSPTGTKIRMLGRGLEVKISEEMLGRIFNPLGDSLDNGPPVIKGEKRDINGSPLNPAAREYPEEFIQTGISAIDGLNALLRGQKLPIFSGSGLPANMLAAQIAKQATVRGEESNFAVVFAAIGARYDDALFFRKFFEETGAINRVAMIVSLANEPPVMKTLTPKTALTLAEYLAFEQDMHVLAILIDMTNYCEALREISAAREEVPGRGGYPGYMYTDLATIYERAGKVIGKKGSITQMPILTMPNDDITHPIPDLTGYITEGQIVLDRALYNKGIYPPINVLMSLSRLAKDGIGEGKTRDDHKDVSNQLFASYARAVDTRGLAAIIGEDSLSEVDRKYLLFGELFERKFVSQGFNENRSIETTLDIGWEILSVLPESELTNIKTQYIQKYHPNYRGKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-10-24" modified="2025-06-18" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UWY8</accession>
    <name>SPEH_SACS2</name>
    <protein>
        <recommendedName>
            <fullName>S-adenosylmethionine decarboxylase proenzyme</fullName>
            <shortName>AdoMetDC</shortName>
            <shortName>SAMDC</shortName>
            <ecNumber>4.1.1.50</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName>S-adenosylmethionine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>S-adenosylmethionine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="primary">speH</name>
        <name type="ordered locus">SSO0585</name>
        <name type="ORF">C21_014</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1991" name="Eur. J. Biochem." volume="199" first="395" last="400">
            <title>S-adenosylmethionine decarboxylase from the thermophilic archaebacterium Sulfolobus solfataricus. Purification, molecular properties and studies on the covalently bound pyruvate.</title>
            <authorList>
                <person name="Cacciapuoti G."/>
                <person name="Porcelli M."/>
                <person name="De Rosa M."/>
                <person name="Gambacorta A."/>
                <person name="Bertoldo C."/>
                <person name="Zappia V."/>
            </authorList>
            <dbReference type="PubMed" id="1649051"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1991.tb16136.x"/>
        </citation>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SCHIFF BASE FORMATION</scope>
        <source>
            <strain>DSM 5833 / MT-4</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2008" name="J. Biol. Chem." volume="283" first="25829" last="25838">
            <title>Crenarchaeal arginine decarboxylase evolved from an S-adenosylmethionine decarboxylase enzyme.</title>
            <authorList>
                <person name="Giles T.N."/>
                <person name="Graham D.E."/>
            </authorList>
            <dbReference type="PubMed" id="18650422"/>
            <dbReference type="DOI" id="10.1074/jbc.M802674200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <scope>MASS SPECTROMETRY</scope>
        <scope>SELF-PROCESSING</scope>
        <scope>CLEAVAGE SITE</scope>
        <scope>PYRUVATE FORMATION AT SER-71</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine. Has no arginine decarboxylase (ArgDC) activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>S-adenosyl-L-methionine + H(+) = S-adenosyl 3-(methylsulfanyl)propylamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:15981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.1.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="2 3">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2 3">Competitively inhibited by methylglyoxal bis-guanylhydrazone. Irreversibly inhibited by NaBH(4) in vitro.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">96 uM for S-adenosyl-L-methionine</KM>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 7.4.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 75 degrees Celsius. Thermostable. Retains 20% activity after incubation at 100 degrees Celsius for 1 hour, 80% after 16 hours at 70 degrees Celsius, while no loss of activity is observed after 16 hours at 50 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.</text>
    </comment>
    <comment type="PTM">
        <text evidence="3">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.</text>
    </comment>
    <comment type="mass spectrometry" mass="6258.0" method="Electrospray" evidence="3">
        <molecule>S-adenosylmethionine decarboxylase alpha chain</molecule>
        <text>Pyruvoyl group-containing alpha subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text>A chimeric protein containing the beta subunit of SSO0536 and the alpha subunit of SSO0585 (SpeH) has ArgDC activity and no AdoMetDC activity, implicating residues responsible for substrate specificity in the beta subunit. But additional factors in the alpha subunit are required for efficient cleavage and turnover.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the prokaryotic AdoMetDC family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.50"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90205">
        <property type="entry name" value="C90205"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UWY8"/>
    <dbReference type="SMR" id="Q9UWY8"/>
    <dbReference type="FunCoup" id="Q9UWY8">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0585"/>
    <dbReference type="PaxDb" id="273057-SSO0585"/>
    <dbReference type="EnsemblBacteria" id="AAK40898">
        <property type="protein sequence ID" value="AAK40898"/>
        <property type="gene ID" value="SSO0585"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0585"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.594"/>
    <dbReference type="eggNOG" id="arCOG00279">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125470_2_1_2"/>
    <dbReference type="InParanoid" id="Q9UWY8"/>
    <dbReference type="PhylomeDB" id="Q9UWY8"/>
    <dbReference type="BRENDA" id="4.1.1.50">
        <property type="organism ID" value="6163"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q9UWY8"/>
    <dbReference type="UniPathway" id="UPA00331">
        <property type="reaction ID" value="UER00451"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004014">
        <property type="term" value="F:adenosylmethionine decarboxylase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.90.10:FF:000005">
        <property type="entry name" value="Arginine decarboxylase proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.90.10">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00464">
        <property type="entry name" value="AdoMetDC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003826">
        <property type="entry name" value="AdoMetDC_fam_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017716">
        <property type="entry name" value="S-AdoMet_deCOase_pro-enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03330">
        <property type="entry name" value="SAM_DCase_Bsu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866:SF2">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02675">
        <property type="entry name" value="AdoMet_dc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <keyword id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_0000030151" description="S-adenosylmethionine decarboxylase beta chain">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000030152" description="S-adenosylmethionine decarboxylase alpha chain">
        <location>
            <begin position="71"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="1">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for catalytic activity" evidence="1">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autolysis">
        <location>
            <begin position="70"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="3">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="1649051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="18650422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="124" mass="14044" checksum="002511155E18D68A" modified="2000-05-01" version="1" precursor="true">MMMGVELAFPKVVGKQVYGSLYECDEDVLKDTKRLEQIIKEAADIGNMNILDIKSWKIGEGVSVVAIILESHITIHTWPEYRFATVDVYSCGPHTSPLNAFRYIVEKLGAKRYTINEADRSSEF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="135" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX05</accession>
    <name>PYRC_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroorotase</fullName>
            <shortName evidence="1">DHOase</shortName>
            <ecNumber evidence="1">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrC</name>
        <name type="ordered locus">SSO0611</name>
        <name type="ORF">C08_039</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90208">
        <property type="entry name" value="C90208"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX05"/>
    <dbReference type="SMR" id="Q9UX05"/>
    <dbReference type="FunCoup" id="Q9UX05">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0611"/>
    <dbReference type="PaxDb" id="273057-SSO0611"/>
    <dbReference type="EnsemblBacteria" id="AAK40922">
        <property type="protein sequence ID" value="AAK40922"/>
        <property type="gene ID" value="SSO0611"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0611"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.619"/>
    <dbReference type="eggNOG" id="arCOG00689">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_1_1_2"/>
    <dbReference type="InParanoid" id="Q9UX05"/>
    <dbReference type="PhylomeDB" id="Q9UX05"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004038">
        <property type="term" value="F:allantoinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01318">
        <property type="entry name" value="DHOase_IIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00220_A">
        <property type="entry name" value="PyrC_classI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004722">
        <property type="entry name" value="DHOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050138">
        <property type="entry name" value="DHOase/Allantoinase_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024403">
        <property type="entry name" value="DHOase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001541">
        <property type="entry name" value="PRK00369.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668:SF2">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12890">
        <property type="entry name" value="DHOase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000147280" description="Dihydroorotase">
        <location>
            <begin position="1"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="56"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="277"/>
            <end position="278"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="1">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00220"/>
        </source>
    </evidence>
    <sequence length="390" mass="44333" checksum="F66C94D6988E41E7" modified="2000-05-01" version="1">MMILWIMGKAYLSKEIRDVCINFDRRIKEIKSICKPDIALPKGTLILPGAIDLHTHIRGLKLAYKEDVVSGTSEASYGGITLIGDMPNSVPFVNTMETITAKLREFEYYSRVDYFVYSGVTKDLKKVDKFPIAGYKIFPEDLEKEETLEVLKSMKLKILHPEVPLALRGNRKLRLNIWYEIGALYYVKGYQNVHITHATNIRTVRLAKELGFTVDITPHHLLVDREKECLTKVNPPIRDTNERLWLLQAINEVDTVVSDHAPHASFEKQQPYEICPPGIAALSFTVPFILTLVSKGIISIDRAVELISTNPARILNIPYGEIKENNYANFTIIQFKDWRYSTKYSKVIETPLDGFPLRASIYMTIIQGKVGSLEGEVFPVKGINPFGENK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UXA8</accession>
    <name>RL3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein uL3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">50S ribosomal protein L3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl3</name>
        <name evidence="1" type="synonym">rpl3Ab</name>
        <name type="ordered locus">SSO0719</name>
        <name type="ORF">C10_011</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L24e.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90220">
        <property type="entry name" value="C90220"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991283.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UXA8"/>
    <dbReference type="SMR" id="Q9UXA8"/>
    <dbReference type="FunCoup" id="Q9UXA8">
        <property type="interactions" value="150"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0719"/>
    <dbReference type="PaxDb" id="273057-SSO0719"/>
    <dbReference type="EnsemblBacteria" id="AAK41018">
        <property type="protein sequence ID" value="AAK41018"/>
        <property type="gene ID" value="SSO0719"/>
    </dbReference>
    <dbReference type="GeneID" id="1454985"/>
    <dbReference type="KEGG" id="sso:SSO0719"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.718"/>
    <dbReference type="eggNOG" id="arCOG04070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033361_2_0_2"/>
    <dbReference type="InParanoid" id="Q9UXA8"/>
    <dbReference type="PhylomeDB" id="Q9UXA8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_A">
        <property type="entry name" value="Ribosomal_uL3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019928">
        <property type="entry name" value="Ribosomal_uL3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03626">
        <property type="entry name" value="L3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003261">
        <property type="entry name" value="PRK04231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000077222" description="Large ribosomal subunit protein uL3">
        <location>
            <begin position="1"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="351" mass="39670" checksum="06D86BA37FA38008" modified="2000-05-01" version="1">MGHRKLASPRRGSAGLRPRKRSSELLPTPRTWPQINSQNPKLLGFVGYKVGMTHVFMIDDWPNSPTNGKEIYMPVTVLEVPPIIPLALRAYAIDGKGEPNVITEYWSSSSLQFLDITRRIHSISSFLKDDESKKKFDERFNTKLDLIKSNLDRIVYFRLLVSTQPRKIPSLGKKAPDLVEIQIGGGEKKSQLDYALNILGKEITIRDVFKEGQLIDVVGVTKGKGFAGVIKRYSVVELPRWHKHRKGSRKIGTRGPSLGTPSYTPQPGQLGFHRRTEYNKRIIKIGDEPKEINPAGGFVRYGIVRNTYVLLEGSILGSKKRPIFLREPVRPSYVFENPPKITYVNLLSQQG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2026-01-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXK6</accession>
    <name>RSMA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable ribosomal RNA small subunit methyltransferase A</fullName>
            <ecNumber evidence="1 5">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">rRNA adenine N-6-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rsmA</name>
        <name evidence="3 6" type="synonym">ksgA</name>
        <name evidence="6" type="ordered locus">SSO0749</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2025" name="Nat. Commun." volume="16" first="348" last="348">
            <title>Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.</title>
            <authorList>
                <person name="Bourgeois G."/>
                <person name="Coureux P.D."/>
                <person name="Lazennec-Schurdevin C."/>
                <person name="Madru C."/>
                <person name="Gaillard T."/>
                <person name="Duchateau M."/>
                <person name="Chamot-Rooke J."/>
                <person name="Bourcier S."/>
                <person name="Mechulam Y."/>
                <person name="Schmitt E."/>
            </authorList>
            <dbReference type="PubMed" id="39753558"/>
            <dbReference type="DOI" id="10.1038/s41467-024-55718-5"/>
        </citation>
        <scope>PROBABLE FUNCTION</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Probably dimethylates adenosine 1475 in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S ribosomal particle; the expected dimethylation of A1476 was not seen (Probable) (PubMed:39753558).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1 5"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90223">
        <property type="entry name" value="E90223"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXK6"/>
    <dbReference type="FunCoup" id="Q7LXK6">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0749"/>
    <dbReference type="PaxDb" id="273057-SSO0749"/>
    <dbReference type="EnsemblBacteria" id="AAK41044">
        <property type="protein sequence ID" value="AAK41044"/>
        <property type="gene ID" value="SSO0749"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0749"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.744"/>
    <dbReference type="eggNOG" id="arCOG04131">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041220_5_0_2"/>
    <dbReference type="InParanoid" id="Q7LXK6"/>
    <dbReference type="PhylomeDB" id="Q7LXK6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031167">
        <property type="term" value="P:rRNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011489">
        <property type="entry name" value="PRK14896.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000463554" description="Probable ribosomal RNA small subunit methyltransferase A">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="11427726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="39753558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="AAK41044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK41044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="194" mass="22600" checksum="3731A0E8C31A9780" modified="2011-05-31" version="1">MDNGIRPILEIGCGKGNITRFLEPDICIELDDKMIEYLKNFNLVIADARYLPVLRGQLVSSLPYQITSDFFKEVIKLNNIRKLTLILQKDFVDKIFNDSTYISFLLNYIYNIQIKDIIPPSCFSPRPKVYSIITIFNRIREYDKEVDSILSCISRYRNKTLRKASKLCGFSSNNDLKVREFKPWQVLELLNSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXN5</accession>
    <name>Q7LXN5_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdopterin-guanine dinucleotide biosynthesis protein B (MobB)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mobB</name>
        <name evidence="2" type="ordered locus">SSO0675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90215">
        <property type="entry name" value="B90215"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991221.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXN5"/>
    <dbReference type="SMR" id="Q7LXN5"/>
    <dbReference type="FunCoup" id="Q7LXN5">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0675"/>
    <dbReference type="PaxDb" id="273057-SSO0675"/>
    <dbReference type="DNASU" id="1454944"/>
    <dbReference type="EnsemblBacteria" id="AAK40977">
        <property type="protein sequence ID" value="AAK40977"/>
        <property type="gene ID" value="SSO0675"/>
    </dbReference>
    <dbReference type="GeneID" id="1454944"/>
    <dbReference type="GeneID" id="44129670"/>
    <dbReference type="KEGG" id="sso:SSO0675"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.675"/>
    <dbReference type="eggNOG" id="arCOG00532">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068199_2_2_2"/>
    <dbReference type="InParanoid" id="Q7LXN5"/>
    <dbReference type="PhylomeDB" id="Q7LXN5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052539">
        <property type="entry name" value="MGD_biosynthesis_adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004435">
        <property type="entry name" value="MobB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00176">
        <property type="entry name" value="mobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40072:SF1">
        <property type="entry name" value="MOLYBDOPTERIN-GUANINE DINUCLEOTIDE BIOSYNTHESIS ADAPTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40072">
        <property type="entry name" value="MOLYBDOPTERIN-GUANINE DINUCLEOTIDE BIOSYNTHESIS ADAPTER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03205">
        <property type="entry name" value="MobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin-guanine dinucleotide biosynthesis protein B (MobB)" evidence="1">
        <location>
            <begin position="4"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="169" mass="18828" checksum="F8CB0E5E5850B1B1" modified="2011-05-31" version="1">MACIFHIIGKKDTGKTSVIESVLREIKKDNLKVAVVKHSHHKLDLAGKDTYRYRNSGSDLILFQEGEEESVLFMPSVSSLTLITLLPVDVILVEGFSNVDIGKKYIINNVNEVEAISKQVINDIKKECQKTNRALILDNVKVEVTSDNALLLTLYNLMKVLGVKNVSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-31" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>Q7LXS5</accession>
    <name>Q7LXS5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UPF0056 membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO0607</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UPF0056 (MarC) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90207">
        <property type="entry name" value="G90207"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991132.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7LXS5"/>
    <dbReference type="STRING" id="273057.SSO0607"/>
    <dbReference type="PaxDb" id="273057-SSO0607"/>
    <dbReference type="EnsemblBacteria" id="AAK40918">
        <property type="protein sequence ID" value="AAK40918"/>
        <property type="gene ID" value="SSO0607"/>
    </dbReference>
    <dbReference type="GeneID" id="1454885"/>
    <dbReference type="KEGG" id="sso:SSO0607"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.615"/>
    <dbReference type="eggNOG" id="arCOG01997">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079909_1_0_2"/>
    <dbReference type="InParanoid" id="Q7LXS5"/>
    <dbReference type="PhylomeDB" id="Q7LXS5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002771">
        <property type="entry name" value="Multi_antbiot-R_MarC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508:SF1">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01914">
        <property type="entry name" value="MarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK40918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="202" mass="21609" checksum="E105AF705A2F27C7" modified="2011-05-31" version="1">MSELVSIPEIAFKLYAIMDPLSILPYLIALYEEFNRNSQNKISWRFLVNKLSIAVTVLLLFFSILGGPLLAFLGISVSALEIGGGIILVYLGVDTLGGFQQLKFLSSRIEEAIITPIATPLIVGPGTMTGLITLSVSNNLIILILGSLLAALLVYLSLLLGPIIIRILGNTGTVAVGRFMAIIIAAFGVQLIIDGISQIKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U05</accession>
    <name>Q97U05_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO3224</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90507">
        <property type="entry name" value="H90507"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010924168.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97U05"/>
    <dbReference type="STRING" id="273057.SSO3224"/>
    <dbReference type="PaxDb" id="273057-SSO3224"/>
    <dbReference type="EnsemblBacteria" id="AAK43319">
        <property type="protein sequence ID" value="AAK43319"/>
        <property type="gene ID" value="SSO3224"/>
    </dbReference>
    <dbReference type="GeneID" id="1453238"/>
    <dbReference type="KEGG" id="sso:SSO3224"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3326"/>
    <dbReference type="eggNOG" id="arCOG03654">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031607_0_0_2"/>
    <dbReference type="InParanoid" id="Q97U05"/>
    <dbReference type="PhylomeDB" id="Q97U05"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="354"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="24"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK43319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="446" mass="48998" checksum="B12F9867328A3358" modified="2001-10-01" version="1">MSTSSISKAQQNQEPKRHLGFIDIVFLSMGSQSPFLSILTYGVEAFIIAGFLAPIAIILGTLLVLLNGLVVYELSKRFTKAGGYYTYAFYSLTKRLGFETGWLYVLYSTTYGVAYIFGTTYILYHILGINPWIVGLGLLSIASFFAIMGIKISTKYAVFTSLLEIGIMTTLALVFLSSTGFHFYNPLSFHVNLGTLAYAILFGSSIPTGYGAIAPVSGEAKDARKTISRAIITVILLGGLLAAFDIYAIGDHLLFYNISANNVDILHLIENRFGLITFIFVLFAAINDGILGSLGFIIATSRTIFAMSYTNLLPKVFSKFENYKGPTNAVILSVILYFITSLFGLYLINNPFVAFGVLGAIALFSSLFVHVAANFSLIKISIKKFRKRLFQIIVGISAVTFSIFELINSLSSASPIEVYIFMSFIIIGFLVAETLSMMEEESEQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q97U23</accession>
    <name>Q97U23_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">SSO3202</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="D90505"/>
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    </dbReference>
    <dbReference type="STRING" id="273057.SSO3202"/>
    <dbReference type="PaxDb" id="273057-SSO3202"/>
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        <property type="gene ID" value="SSO3202"/>
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    <dbReference type="KEGG" id="sso:SSO3202"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3304"/>
    <dbReference type="eggNOG" id="arCOG02569">
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    <dbReference type="HOGENOM" id="CLU_015263_5_3_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902476">
        <property type="term" value="P:chloride transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04594">
        <property type="entry name" value="CBS_pair_voltage-gated_CLC_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Voltage_gated_ClC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000018">
        <property type="entry name" value="Chloride transporter, ClC family"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050368">
        <property type="entry name" value="ClC-type_chloride_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43427">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43427:SF6">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="8" id="KW-0868">Chloride</keyword>
    <keyword evidence="7" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <end position="47"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <end position="104"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <end position="203"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="215"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="291"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="327"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="360"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="469"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="536"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAK43299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="595" mass="64147" checksum="B77F28838C000E7F" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UG4</accession>
    <name>Q97UG4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Maltose ABC transporter, permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO3058</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="F90487"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UG4"/>
    <dbReference type="FunCoup" id="Q97UG4">
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    </dbReference>
    <dbReference type="STRING" id="273057.SSO3058"/>
    <dbReference type="TCDB" id="3.A.1.5.8">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="PaxDb" id="273057-SSO3058"/>
    <dbReference type="EnsemblBacteria" id="AAK43157">
        <property type="protein sequence ID" value="AAK43157"/>
        <property type="gene ID" value="SSO3058"/>
    </dbReference>
    <dbReference type="GeneID" id="1453076"/>
    <dbReference type="KEGG" id="sso:SSO3058"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3155"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_0_2"/>
    <dbReference type="InParanoid" id="Q97UG4"/>
    <dbReference type="PhylomeDB" id="Q97UG4"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163:SF6">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="98"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK43157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="322" mass="35108" checksum="5C6D72904B067970" modified="2001-10-01" version="1">MATFFVVITVNFILPRLMPGSALAVVISLIEGQNGVAATAGSSAIALEVSQLEAAFGLTPAPWYVQYAHYIQGIFTLNLGASITYYPTPVLSIILSGLPWTLFLVVVGAIIAFFLGSYLGSIAGFNRRSRKDLVILIITSILAALPSFVILMYLEMWLSVQHGIFLISFPSKINTSPNSIMALSRFYTLPIIALMFTSLSGFVLGMRNNMLHTIRDNYVTYAEILGLDHNRIRKMVYKNSLLPNITAFAIILGLAISQSLTVEGLLTTPGVGFFFGLALTSKDLPLLQGIFLFIAIMLILSIAIVEAIYTILDPRARVSESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="122" xmlns="https://uniprot.org/uniprot">
    <accession>Q97UK6</accession>
    <name>Q97UK6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-oxoacyl-(Acyl carrier protein) reductase (FabG-9)</fullName>
            <ecNumber evidence="1">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fabG-9</name>
        <name evidence="1" type="ordered locus">SSO3004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.100" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK43107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90481">
        <property type="entry name" value="D90481"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992654.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97UK6"/>
    <dbReference type="FunCoup" id="Q97UK6">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO3004"/>
    <dbReference type="PaxDb" id="273057-SSO3004"/>
    <dbReference type="EnsemblBacteria" id="AAK43107">
        <property type="protein sequence ID" value="AAK43107"/>
        <property type="gene ID" value="SSO3004"/>
    </dbReference>
    <dbReference type="GeneID" id="1453026"/>
    <dbReference type="KEGG" id="sso:SSO3004"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.3096"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_2"/>
    <dbReference type="InParanoid" id="Q97UK6"/>
    <dbReference type="PhylomeDB" id="Q97UK6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052625">
        <property type="entry name" value="Chl_b_Red"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24314">
        <property type="entry name" value="NON-SPECIFIC LIPID TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24314:SF20">
        <property type="entry name" value="STEROL CARRIER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK43107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="299" mass="33097" checksum="552501D22F11E0D5" modified="2001-10-01" version="1">MKKVSIITGGAKGIGAAIGYRLGKQGYAVVIADVDEEAGKYRENHFRGEGIDSFFIKTDVSSEMDVSNMVEKVYGRYGRIDVLVNNAGIGFSGKSIEEQTLDEWRRVIETNLTGVWLCSKYAVKYMKNNGGVIVNIASTRAFQSEPNTEPYSASKGGIIALTHSLAVSLSKYNIRVVSISPGWIDTSRWQVPPRESTLSGLDHGQHLTRRVGKPEDVASLVTFLVSDDASWISGVNFTIDGGMTVKMIYIDENVIQDSLTMLFNDVEVSYQIRKLIEKIKKADNKENIKSKLDELLRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="134" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V00</accession>
    <name>Q97V00_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter, ATP binding protein (Branched chain amino acid)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">SSO2838</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90461">
        <property type="entry name" value="D90461"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923991.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V00"/>
    <dbReference type="FunCoup" id="Q97V00">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2838"/>
    <dbReference type="PaxDb" id="273057-SSO2838"/>
    <dbReference type="EnsemblBacteria" id="AAK42947">
        <property type="protein sequence ID" value="AAK42947"/>
        <property type="gene ID" value="SSO2838"/>
    </dbReference>
    <dbReference type="GeneID" id="1452866"/>
    <dbReference type="KEGG" id="sso:SSO2838"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2923"/>
    <dbReference type="eggNOG" id="arCOG00924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q97V00"/>
    <dbReference type="PhylomeDB" id="Q97V00"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015803">
        <property type="term" value="P:branched-chain amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015807">
        <property type="term" value="P:L-amino acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03224">
        <property type="entry name" value="ABC_TM1139_LivF_branched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052156">
        <property type="entry name" value="BCAA_Transport_ATP-bd_LivF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43820:SF7">
        <property type="entry name" value="BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43820">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="2"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="236" mass="26441" checksum="55CFD3A4E479B6BA" modified="2001-10-01" version="1">MLETVKLNAGYGKLQILFDVDFIASKKQIITIVGPNGSGKSTFLKTLVGLTNVISGKIIFEGDDITRFPPYKRSRKGLVYVAQIDNVFRSLTVEENFKLSSYNLSENEYNERMEIIFTLFSQIRDILKRKVYQLSGGQRQMVAISLALLRKPKILLLDEPTAQLAPKIANEVFKKIVEIRDLTEVGIILVEQNAKKALEIADNAYLFTSGRVLFKGKPVELLSNPDLGKIFLGLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V64</accession>
    <name>Q97V64_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90453">
        <property type="entry name" value="B90453"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990228.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V64"/>
    <dbReference type="STRING" id="273057.SSO2772"/>
    <dbReference type="PaxDb" id="273057-SSO2772"/>
    <dbReference type="DNASU" id="1452800"/>
    <dbReference type="EnsemblBacteria" id="AAK42881">
        <property type="protein sequence ID" value="AAK42881"/>
        <property type="gene ID" value="SSO2772"/>
    </dbReference>
    <dbReference type="GeneID" id="1452800"/>
    <dbReference type="KEGG" id="sso:SSO2772"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2853"/>
    <dbReference type="eggNOG" id="arCOG06010">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110562_0_0_2"/>
    <dbReference type="InParanoid" id="Q97V64"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031594">
        <property type="entry name" value="OFeT_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16955">
        <property type="entry name" value="OFeT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="208" mass="22703" checksum="25BCA2BD0B550C8C" modified="2001-10-01" version="1">MDVSVFLAALGISLLELTEAGAIAAIYHNIYKNNLPFIYAVLGVAIVLIPTFTLGKLIYLVPLNYVLLASAVILFYFGYRLIRSARRSFKGIKKKGGEEKEEGLSVVFTVSAIEGLEASLVILALIPQSYSSAVMGAILASVLVVILTAILKSQVARIRLPHLKFVLSALLFSLGTLWISEVVFDIPDLILPLFFLAYLGINYVIIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V71</accession>
    <name>Q97V71_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Electron transfer flavoprotein, subunit beta (EtfB)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">etfB</name>
        <name evidence="2" type="ordered locus">SSO2763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90452">
        <property type="entry name" value="C90452"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990241.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V71"/>
    <dbReference type="FunCoup" id="Q97V71">
        <property type="interactions" value="214"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2763"/>
    <dbReference type="PaxDb" id="273057-SSO2763"/>
    <dbReference type="EnsemblBacteria" id="AAK42874">
        <property type="protein sequence ID" value="AAK42874"/>
        <property type="gene ID" value="SSO2763"/>
    </dbReference>
    <dbReference type="GeneID" id="1452793"/>
    <dbReference type="KEGG" id="sso:SSO2763"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2844"/>
    <dbReference type="eggNOG" id="arCOG00446">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060196_2_2_2"/>
    <dbReference type="InParanoid" id="Q97V71"/>
    <dbReference type="PhylomeDB" id="Q97V71"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000072">
        <property type="entry name" value="Protein FixA homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF20">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN, SUBUNIT BETA (ETFB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="1">
        <location>
            <begin position="19"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="242" mass="26560" checksum="E02DEEB7F5BB4B6C" modified="2001-10-01" version="1">MNIIVGFKIVPDDQMIKVVGDKLNTDAPLKVSTYDKNAIEEAIRLKEKFGGKVTGITVGNNDRKSIREALAMGVDEVIAILVKDPDVYVTAMAIAENVKTLNPDIILFSEMTTDSGTSALPAYVSELLGLPYISNVKSLKIEGNKVVADRGMVSYLETVESSLPIIVSVGGEINTPRIPSVKQIMESSKKPVKEVKFDAQSKVRIREIKPMIVNRKRIVIEESDINKAVDKLIEYLKSDGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="102" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V91</accession>
    <name>Q97V91_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2744</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90449">
        <property type="entry name" value="G90449"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V91"/>
    <dbReference type="STRING" id="273057.SSO2744"/>
    <dbReference type="PaxDb" id="273057-SSO2744"/>
    <dbReference type="EnsemblBacteria" id="AAK42854">
        <property type="protein sequence ID" value="AAK42854"/>
        <property type="gene ID" value="SSO2744"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2744"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2823"/>
    <dbReference type="eggNOG" id="arCOG01290">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015525_2_2_2"/>
    <dbReference type="InParanoid" id="Q97V91"/>
    <dbReference type="PhylomeDB" id="Q97V91"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040086">
        <property type="entry name" value="MJ0683-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43432:SF6">
        <property type="entry name" value="RADICAL SAM CORE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43432">
        <property type="entry name" value="SLR0285 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01084">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="4">
        <location>
            <begin position="28"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="261" mass="30276" checksum="31BBFDC6497CEA5A" modified="2001-10-01" version="1">MVKMIVKSIRVKSALSKSGLKELNYSLNPYLGCVFSCPYCYAPNFTPDREASENWGKVVAVKENLLEVLEREVKIYKRGVVGISTITDAYQPIEALRKLTRESLKILLKNGFRVSIQTKSPLVLRDIDVLLEYKDRVDVGFTVTSLNNKLEPNAPPAKGRLRALEELSNEGLETWIFLGPIIQGVNDTEVENIIREISNIKARVIFDSFHYYRGLKFKEGSIHWWVNLKNKILENCKRYSIECHEQSEDWIYEKKRYYKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="113" xmlns="https://uniprot.org/uniprot">
    <accession>Q97V96</accession>
    <name>Q97V96_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="6">4-hydroxybutyryl-CoA dehydratase (AbfD-2)</fullName>
            <ecNumber evidence="6">4.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">abfD-2</name>
        <name evidence="6" type="ordered locus">SSO2738</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.-" evidence="6"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90449">
        <property type="entry name" value="B90449"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990277.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97V96"/>
    <dbReference type="SMR" id="Q97V96"/>
    <dbReference type="FunCoup" id="Q97V96">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2738"/>
    <dbReference type="PaxDb" id="273057-SSO2738"/>
    <dbReference type="EnsemblBacteria" id="AAK42849">
        <property type="protein sequence ID" value="AAK42849"/>
        <property type="gene ID" value="SSO2738"/>
    </dbReference>
    <dbReference type="GeneID" id="1452768"/>
    <dbReference type="KEGG" id="sso:SSO2738"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2818"/>
    <dbReference type="eggNOG" id="arCOG02143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023920_1_0_2"/>
    <dbReference type="InParanoid" id="Q97V96"/>
    <dbReference type="PhylomeDB" id="Q97V96"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3140.10">
        <property type="entry name" value="4-hydroxybutyryl-coa dehydratase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004925">
        <property type="entry name" value="HpaB/PvcC/4-BUDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024719">
        <property type="entry name" value="HpaB/PvcC/4-BUDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024674">
        <property type="entry name" value="HpaB/PvcC/4-BUDH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36117">
        <property type="entry name" value="4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36117:SF3">
        <property type="entry name" value="4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03241">
        <property type="entry name" value="HpaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11794">
        <property type="entry name" value="HpaB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000331">
        <property type="entry name" value="HpaA_HpaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpaB/PvcC/4-BUDH N-terminal" evidence="5">
        <location>
            <begin position="5"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="HpaB/PvcC/4-BUDH C-terminal" evidence="4">
        <location>
            <begin position="282"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK42849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="502" mass="56726" checksum="1526325F42FEB3AE" modified="2001-10-01" version="1">MVLRTGEEYVNAIRHRNKVEIYVMGKEVKDVTEHPFLKPSVLAFKATYDAAHEEDSESLARAWSSFINEEVNRFTHIHRSPEDLAAKVKLLRKISHKTGACFQRCVGWDALNAIYVTSRLMAERGKKEYYERFVEYLKYVQKNDVALAGAMTDVKGIRTLRPHEQPHAYLRVTQVTKDGIYVSGAKANITGIGAAEEIVVMPTRAMSEKDMDFAVAFSIPLDTEGIKVIIGRQVNDGRRLEEGEIDGLPYRTNHEGLVIFDNVFVPMERVFMLGDWQMSGILVEIFSSYHRQGYGGCKSGLGDVIIGAAYNLAKQIGVEKAPHIQNKLNEMVLLNENMYGSGIAASLDAVKLCEDGCWWVNPMFANVTKQLVTRFPYEITKFSTDIAGGIIGTAPSEWDLKNPKLKPYIEKYLQGMMDYSTEDRLRMVRLLENVGFSVAFLIESVHGAGSPEAQRIAINRVYDYEYAEKIAKYLSQIEKRIDFKENAEPWRKTDTEKIASSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="101" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VC7</accession>
    <name>Q97VC7_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Regucalcin homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2705</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMP-30/CGR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90445">
        <property type="entry name" value="B90445"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988632.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VC7"/>
    <dbReference type="FunCoup" id="Q97VC7">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2705"/>
    <dbReference type="PaxDb" id="273057-SSO2705"/>
    <dbReference type="EnsemblBacteria" id="AAK42817">
        <property type="protein sequence ID" value="AAK42817"/>
        <property type="gene ID" value="SSO2705"/>
    </dbReference>
    <dbReference type="GeneID" id="1452736"/>
    <dbReference type="KEGG" id="sso:SSO2705"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2786"/>
    <dbReference type="eggNOG" id="arCOG05370">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036110_3_1_2"/>
    <dbReference type="InParanoid" id="Q97VC7"/>
    <dbReference type="PhylomeDB" id="Q97VC7"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004341">
        <property type="term" value="F:gluconolactonase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019853">
        <property type="term" value="P:L-ascorbic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000139">
        <property type="entry name" value="SMP-30/Gluconolaconase/LRE domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005511">
        <property type="entry name" value="SMP-30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907:SF47">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01790">
        <property type="entry name" value="SMP30FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="4">
        <location>
            <begin position="15"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="285" mass="32195" checksum="3FA045545790DA5B" modified="2001-10-01" version="1">MEPNLITLSNYKATLGEGPVYDKELNKLFWVDIEGKRIIVNDLNTGTEVFYEMPDLISSLCVIDDKRVIATIRHGFYIVNLDRGEIQKIAEVETDMESNRFNDGKCDKLGRYWAGTMNMNERKPTGSFYKLDTGKITKILEGLTVSNGLGWDPDNTQMFLIDSPVRKVFVFDFDLNKGEIYNRRVAVDFGNEPGNPDGMAVDEEGYIWVAHWGGGKVSRWNPKTGKKLFEIKVPATYVTSVTFGTQELDQLFITTAGKSQDPLAGKTFTTKANVRGLQNFRFKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VG4</accession>
    <name>Q97VG4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO2662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90440">
        <property type="entry name" value="E90440"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923947.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VG4"/>
    <dbReference type="STRING" id="273057.SSO2662"/>
    <dbReference type="PaxDb" id="273057-SSO2662"/>
    <dbReference type="EnsemblBacteria" id="AAK42780">
        <property type="protein sequence ID" value="AAK42780"/>
        <property type="gene ID" value="SSO2662"/>
    </dbReference>
    <dbReference type="GeneID" id="1452699"/>
    <dbReference type="KEGG" id="sso:SSO2662"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2744"/>
    <dbReference type="eggNOG" id="arCOG04031">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870836_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VG4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="136" mass="14867" checksum="C9012D9220EF4CFA" modified="2001-10-01" version="1">MSRLAVSQSRIRPSRLMLYISLTETILLAGLFYAGLATLFYDKFPLNAYSEALILSSHITLAMLVGFFGSAMLAQSVREGIRSVYLFSLLNLLFIGIAAAGGLAFYGLLIPDYAYLMALGFFGSLFCTSSVLFYAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VH6</accession>
    <name>Q97VH6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter, ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">SSO2647</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90439">
        <property type="entry name" value="A90439"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009988557.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VH6"/>
    <dbReference type="FunCoup" id="Q97VH6">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2647"/>
    <dbReference type="PaxDb" id="273057-SSO2647"/>
    <dbReference type="EnsemblBacteria" id="AAK42768">
        <property type="protein sequence ID" value="AAK42768"/>
        <property type="gene ID" value="SSO2647"/>
    </dbReference>
    <dbReference type="GeneID" id="1452687"/>
    <dbReference type="KEGG" id="sso:SSO2647"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2730"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q97VH6"/>
    <dbReference type="PhylomeDB" id="Q97VH6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF18">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK42768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="309" mass="34907" checksum="D1FE3037A1075041" modified="2001-10-01" version="1">MIKTIDISKTFKIKNKEIKAVDDVSFEIERNSTGALVGPNGAGKTTIIKMLSTLIIPSSGDALVNGYSITKEEKKVRESIGLVTVSERLFYYRLTALENLIFFASLQNLSLSDARKRAKEVLEIVGLSEWGNIPYMKFSTGMQRKLALARALITDPPVLLLDEPTLGLDPLSARMFRDLVRRIGRDKTILLTSHYMKDIEELAEKIILLKKGKAIAEGNKDNLKNYLGKVIEVEVNEYPLTLGKYVVQTSTNYYILRIPEKELDELKDYRVIKEEEPSLEDVYIYLIGDVEDSARLEAVRRGGWGGRWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VT2</accession>
    <name>Q97VT2_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Metabolite transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">SSO2528</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90425">
        <property type="entry name" value="C90425"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990972.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VT2"/>
    <dbReference type="FunCoup" id="Q97VT2">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2528"/>
    <dbReference type="PaxDb" id="273057-SSO2528"/>
    <dbReference type="EnsemblBacteria" id="AAK42658">
        <property type="protein sequence ID" value="AAK42658"/>
        <property type="gene ID" value="SSO2528"/>
    </dbReference>
    <dbReference type="GeneID" id="1453985"/>
    <dbReference type="KEGG" id="sso:SSO2528"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2604"/>
    <dbReference type="eggNOG" id="arCOG02691">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_5_2"/>
    <dbReference type="InParanoid" id="Q97VT2"/>
    <dbReference type="PhylomeDB" id="Q97VT2"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045:SF1">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="367"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="394"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="7"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAK42658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="426" mass="45936" checksum="F5B8E4498246AB44" modified="2001-10-01" version="1">MKDETKVSIAAMIGIAFEFYDFLIFGFVSSILASLFFPSTNKIVSLLDTLAVFATGFAGRPLGAIVFGHLGDKIGRKYTLIVTMSLMGLSSLFTGLLPSYAVLGILAPTLLTVLRLLQGFSLGGEFGGGITLSAEFAKPTNRAFYIGIAQMAQGVGPLMATGLIFIFSSIMSPPAFASTGWRILFIIGAFIAVIGVIIRLKISESPVFKNVREMGQISKFPLAEAFRLYWKRILLGLGFIIGGTTLTYATSVFAASYLETVIGVPAKTVSLALTIGYIVEAICILAFSLLADKIGRKPMMITTAVGLLILVYPYFYLISTGQFSLILLAQILYSTIGSFSTAAYAAALTELFPTKVRYTALSFDYHVGVAVFGGTTPFIASYLIYATGYKLAPVYWGIAGMVITLIAYLLYKETKGTIFEGQERVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VX8</accession>
    <name>Q97VX8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO10449</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90419">
        <property type="entry name" value="E90419"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923880.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VX8"/>
    <dbReference type="SMR" id="Q97VX8"/>
    <dbReference type="PaxDb" id="273057-SSO10449"/>
    <dbReference type="EnsemblBacteria" id="AAK42612">
        <property type="protein sequence ID" value="AAK42612"/>
        <property type="gene ID" value="SSO10449"/>
    </dbReference>
    <dbReference type="GeneID" id="15296746"/>
    <dbReference type="KEGG" id="sso:SSO10449"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2552"/>
    <dbReference type="eggNOG" id="arCOG01055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159725_2_0_2"/>
    <dbReference type="InParanoid" id="Q97VX8"/>
    <dbReference type="PhylomeDB" id="Q97VX8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038723">
        <property type="entry name" value="ArnR1-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14947">
        <property type="entry name" value="HTH_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArnR1-like winged helix-turn-helix" evidence="1">
        <location>
            <begin position="4"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="95" mass="11072" checksum="1027395B64731D18" modified="2001-10-01" version="1">MAKKRSKLEIIQAILEACKSGSPKTRIMYGANLSYALTGRYIKMLMDLEIIKQEGKQYMLTKKGEELLEDIRRFNDMRKNMDQLKEKINSVLSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q97VZ4</accession>
    <name>Q97VZ4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO2457</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90417">
        <property type="entry name" value="E90417"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923864.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97VZ4"/>
    <dbReference type="STRING" id="273057.SSO2457"/>
    <dbReference type="PaxDb" id="273057-SSO2457"/>
    <dbReference type="EnsemblBacteria" id="AAK42596">
        <property type="protein sequence ID" value="AAK42596"/>
        <property type="gene ID" value="SSO2457"/>
    </dbReference>
    <dbReference type="GeneID" id="15296728"/>
    <dbReference type="KEGG" id="sso:SSO2457"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2534"/>
    <dbReference type="eggNOG" id="arCOG00423">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076809_0_0_2"/>
    <dbReference type="InParanoid" id="Q97VZ4"/>
    <dbReference type="PhylomeDB" id="Q97VZ4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052968">
        <property type="entry name" value="Nucleotide_metab_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42146">
        <property type="entry name" value="3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42146:SF1">
        <property type="entry name" value="OLIGORIBONUCLEASE NRNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDH" evidence="1">
        <location>
            <begin position="6"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="239"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK42596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="329" mass="37422" checksum="096DC11C46F095B0" modified="2001-10-01" version="1">MEYYAIVHNDFDGTASAAVYARAIKSLPKNVFFTEPNKLHSLLASLELRGVYNVMIADLGINASTFNEIIRNLKKLIDQGANVEWFDHHVWKDEWKEELKKIGVSVYHDTSTCGAGVIYKYKNPNDEFSRRLSSADCSVDIWLHDDPMGEKLRRIVENNKDYSWKNELIKMFYNGILWDDTFDKMLEEVVSRELEGYKKVMKSYKLIQINGHKVVVAVRWKGPPDISYASQFLMTRTDASVFVSANGKSISFRSKEIDVRQFAVKLGGGGHPLAAGAQLKVPIIYRFLNRIGIKGPMLNWVTKIVQNTIADVGFITYQESKKSSASSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="115" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W61</accession>
    <name>Q97W61_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetyl-CoA c-acetyltransferase (Acetoacetyl-CoA thiolase) (AcaB-4)</fullName>
            <ecNumber evidence="7">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">acaB-4</name>
        <name evidence="7" type="ordered locus">SSO2377</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.9" evidence="7"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90408">
        <property type="entry name" value="H90408"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W61"/>
    <dbReference type="FunCoup" id="Q97W61">
        <property type="interactions" value="316"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO2377"/>
    <dbReference type="PaxDb" id="273057-SSO2377"/>
    <dbReference type="EnsemblBacteria" id="AAK42527">
        <property type="protein sequence ID" value="AAK42527"/>
        <property type="gene ID" value="SSO2377"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO2377"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.2461"/>
    <dbReference type="eggNOG" id="arCOG01282">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_0_0_2"/>
    <dbReference type="InParanoid" id="Q97W61"/>
    <dbReference type="PhylomeDB" id="Q97W61"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF107">
        <property type="entry name" value="ACETYL-COA ACETYLTRANSFERASE, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="5">
        <location>
            <begin position="5"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="6">
        <location>
            <begin position="271"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAK42527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="397" mass="42855" checksum="B2AE787015E250B3" modified="2001-10-01" version="1">MPESVYIASAVRTPIGKFGGALRNLSPVDLGSIVIREALRRANVEPGKIDMAIMGNVLRAGHGQDIARQCAISAGIPFEIDGFSVDMVCSSGMISVITASQMIKSGDADIIVAGGTENMSQAMFAIKSDIRWGVKMLMNRNIELIDTMLYDGLTDPFQYKVMGQEADMVAKSHNISRKELDEVAYQSHLRAHKATVNGYFKSEIVEIKADGKVINTDEGIRADTSLDKLSSLPPAFTDDGLHTAGNSSQISDGAAALVLVSEKAAKELKIEPIARILGYSWVGIESWRFTEAPIFAIKKLLSKLDTDINHFDYFENNEAFAVNNVLINRYLGIPYDRLNVFGGAIALGHPIGASGARIIVTLLNVLSKMHGTRGIASICHGIGGSTAIAIELLKEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-10-02" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97W98</accession>
    <name>Q97W98_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ORF D-335-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO10237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90404">
        <property type="entry name" value="C90404"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97W98"/>
    <dbReference type="PaxDb" id="273057-SSO10237"/>
    <dbReference type="EnsemblBacteria" id="AAK42490">
        <property type="protein sequence ID" value="AAK42490"/>
        <property type="gene ID" value="SSO10237"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO10237"/>
    <dbReference type="eggNOG" id="arCOG07277">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167324_2_0_2"/>
    <dbReference type="InParanoid" id="Q97W98"/>
    <dbReference type="PhylomeDB" id="Q97W98"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012922">
        <property type="entry name" value="ORF_D-335"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07935">
        <property type="entry name" value="SSV1_ORF_D-335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ORF D-335-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="90" mass="10208" checksum="5AE75F77EFED0635" modified="2001-10-01" version="1">MGNKVFTFGDIRIREVKGKYYVYLIEKDSEGKRRDNYVGPLDEIVKGYMRGTGFEPVQAFASGASVRPLCPSSGTPAYQYFILTRYKVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X33</accession>
    <name>Q97X33_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90357">
        <property type="entry name" value="D90357"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990338.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X33"/>
    <dbReference type="SMR" id="Q97X33"/>
    <dbReference type="PaxDb" id="273057-SSO1921"/>
    <dbReference type="EnsemblBacteria" id="AAK42115">
        <property type="protein sequence ID" value="AAK42115"/>
        <property type="gene ID" value="SSO1921"/>
    </dbReference>
    <dbReference type="GeneID" id="7798816"/>
    <dbReference type="KEGG" id="sso:SSO1921"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1988"/>
    <dbReference type="eggNOG" id="arCOG03706">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153003_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X33"/>
    <dbReference type="PhylomeDB" id="Q97X33"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="3"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="136" mass="15649" checksum="4369C4DD93418879" modified="2001-10-01" version="1">MSIFVDTNVIISFVEQDVNYNKALKIREINDLMTSEVTVLELYSFFSRKLKDEILAEASTKYALKVSNVKVVELDMNRLFRKSLELSTKLQLKTLDVLQISSALLLNAKSFITFDKDILKKRDLIEKIVKISVSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>Q97X79</accession>
    <name>Q97X79_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO1875</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H90350">
        <property type="entry name" value="H90350"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992688.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97X79"/>
    <dbReference type="STRING" id="273057.SSO1875"/>
    <dbReference type="PaxDb" id="273057-SSO1875"/>
    <dbReference type="EnsemblBacteria" id="AAK42063">
        <property type="protein sequence ID" value="AAK42063"/>
        <property type="gene ID" value="SSO1875"/>
    </dbReference>
    <dbReference type="GeneID" id="1453390"/>
    <dbReference type="KEGG" id="sso:SSO1875"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1926"/>
    <dbReference type="eggNOG" id="arCOG03862">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1529295_0_0_2"/>
    <dbReference type="InParanoid" id="Q97X79"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK42063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="175" mass="20260" checksum="EFDE007822988124" modified="2001-10-01" version="1">MAYRCCETTFETERDFKKHIRSKYWHRINHSDSLNLRIRNDVLYNILRNPKLLFGISNVIVPIQDKAIVYLPLSKDKTGLVLKRSIVGKLEGPKLNIDLIEYKFTSDKLIYNFGFKVRKINEENSQLVILSSMMVNIGILGRLLPSAVIKELQKMVTPQVFVDKYLSKNISSLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-01-24" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XA1</accession>
    <name>Q97XA1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">First ORF in transposon ISC1359</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO1850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90348">
        <property type="entry name" value="B90348"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XA1"/>
    <dbReference type="STRING" id="273057.SSO1850"/>
    <dbReference type="PaxDb" id="273057-SSO1850"/>
    <dbReference type="EnsemblBacteria" id="AAK42041">
        <property type="protein sequence ID" value="AAK42041"/>
        <property type="gene ID" value="SSO1850"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1850"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1904"/>
    <dbReference type="eggNOG" id="arCOG03902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067987_1_0_2"/>
    <dbReference type="InParanoid" id="Q97XA1"/>
    <dbReference type="PhylomeDB" id="Q97XA1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS4-like" evidence="1">
        <location>
            <begin position="114"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="282" mass="32683" checksum="A03A4C09902DE99E" modified="2001-10-01" version="1">MKYRQNREDENFCRKVCIYEGGLEHMKHENTTKPAKFNRDFARSALKIIYSVLTKILFPEELLSALLKASGSYLSRLGKDGRRALRKLNAVQVEDVRDALRKMGRMTLRGVRDRRVAVDFHAIPQYHADKSFLSRIKPTKGTSWGLVQAAIFLLGRTRSFLDVIPVTVKNVAEGFKAVMEVIVKELEEDKLRLVMVFADREFAVNEVIRYLLELGLDFVISAKAQMYKKYKGMLQDVDVSFGGVRYIWISLRETWEWSLSHYPEEGRRQDYCFPREEGDGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XA9</accession>
    <name>Q97XA9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase, putative amino-end</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO9043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK42032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90347">
        <property type="entry name" value="A90347"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XA9"/>
    <dbReference type="PaxDb" id="273057-SSO9043"/>
    <dbReference type="EnsemblBacteria" id="AAK42032">
        <property type="protein sequence ID" value="AAK42032"/>
        <property type="gene ID" value="SSO9043"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO9043"/>
    <dbReference type="eggNOG" id="arCOG02759">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2613804_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XA9"/>
    <dbReference type="PhylomeDB" id="Q97XA9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1290">
        <property type="entry name" value="Transposase IS200-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002686">
        <property type="entry name" value="Transposase_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036515">
        <property type="entry name" value="Transposase_17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33360:SF3">
        <property type="entry name" value="PUTATIVE AMINO-END-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33360">
        <property type="entry name" value="TRANSPOSASE FOR INSERTION SEQUENCE ELEMENT IS200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01797">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143422">
        <property type="entry name" value="Transposase IS200-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS200-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK42032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="78" mass="8928" checksum="EF8FC595B88563D6" modified="2001-10-01" version="1">MPKYLRSTLVDEDVEYTKEILRSIAEELSCKIISLEVMPNHIHLFVNCPSRYVPSYLAKTTLRLLVTVQINMGTRGMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-06-18" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>Q97XP1</accession>
    <name>Q97XP1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase ISC1316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">SSO1672</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C90328">
        <property type="entry name" value="C90328"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97XP1"/>
    <dbReference type="PaxDb" id="273057-SSO1672"/>
    <dbReference type="EnsemblBacteria" id="AAK41882">
        <property type="protein sequence ID" value="AAK41882"/>
        <property type="gene ID" value="SSO1672"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO1672"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1719"/>
    <dbReference type="eggNOG" id="arCOG00684">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_0_0_2"/>
    <dbReference type="InParanoid" id="Q97XP1"/>
    <dbReference type="PhylomeDB" id="Q97XP1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF11">
        <property type="entry name" value="RNA-GUIDED DNA ENDONUCLEASE RV2885C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="175"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="300"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAK41882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="395" mass="46590" checksum="017D2BA24EA27DFD" modified="2001-10-01" version="1">MPTLGFRFRAYTDEQTLRALKAQLKLACKIYNTLRWADIYFYQRDGKGLTQTELRQLALDLRKQDDEYKKLYSQVVQQIADRYYEARQRFFDGLARFPKERKPHKYYSLVYTQSGWKILQVREIRKGSKKKLITLKLSNLGTFKVIVHRDFPLDKVKRVIVKLTRSERIYITFVVDHEFPKLPNTGKVVAIDVGVEKLLVTSDGEYFPNLRPYEKALWKVKHLHRELSRKKFLSNNWFKAKVKLARAYEYLKNLRTDLYMKLGKWFAEHYDVVVMEDIHVKQLIGKSLRSLRRRLSDVAFSELRDLIKYQLEKYGKKLILVNPAYTSKTCAKCGYVKEDLSLSDRVFVCPNCGWIADRDYNASLNILRGSGSERPLVWSSALYQYQHFGTGMAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YB4</accession>
    <name>Q97YB4_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Partial ORF in transposon ISC1491</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO7998</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90299">
        <property type="entry name" value="B90299"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YB4"/>
    <dbReference type="PaxDb" id="273057-SSO7998"/>
    <dbReference type="EnsemblBacteria" id="AAK41649">
        <property type="protein sequence ID" value="AAK41649"/>
        <property type="gene ID" value="SSO7998"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO7998"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1439"/>
    <dbReference type="eggNOG" id="arCOG03585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180515_0_0_2"/>
    <dbReference type="InParanoid" id="Q97YB4"/>
    <dbReference type="PhylomeDB" id="Q97YB4"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="76" mass="8950" checksum="8DEC9FE4D56D5A22" modified="2001-10-01" version="1">MESEIKTRGFAIDVSRDKLTVAKAELVITQEKREVEVNEVREFKYDNEGIEELIKFLGEYKEGIMESIGPYFFYLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-10-08" version="106" xmlns="https://uniprot.org/uniprot">
    <accession>Q97YU6</accession>
    <name>Q97YU6_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbon monoxide dehydrogenase, large chain (CutA-1)</fullName>
            <ecNumber evidence="4">1.2.7.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cutA-1</name>
        <name evidence="4" type="ordered locus">SSO1209</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.7.4" evidence="4"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90275">
        <property type="entry name" value="B90275"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010923249.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97YU6"/>
    <dbReference type="FunCoup" id="Q97YU6">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1209"/>
    <dbReference type="PaxDb" id="273057-SSO1209"/>
    <dbReference type="EnsemblBacteria" id="AAK41457">
        <property type="protein sequence ID" value="AAK41457"/>
        <property type="gene ID" value="SSO1209"/>
    </dbReference>
    <dbReference type="GeneID" id="1454236"/>
    <dbReference type="KEGG" id="sso:SSO1209"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1208"/>
    <dbReference type="eggNOG" id="arCOG01167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_2_0_2"/>
    <dbReference type="InParanoid" id="Q97YU6"/>
    <dbReference type="PhylomeDB" id="Q97YU6"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:anaerobic carbon-monoxide dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.50">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.365.10">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046867">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908:SF132">
        <property type="entry name" value="ALDEHYDE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="MoCoBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20256">
        <property type="entry name" value="MoCoBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde oxidase/xanthine dehydrogenase a/b hammerhead" evidence="3">
        <location>
            <begin position="10"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK41457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="688" mass="75078" checksum="B2B0C8002EDB45A7" modified="2001-10-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="131" xmlns="https://uniprot.org/uniprot">
    <accession>Q97Z14</accession>
    <name>Q97Z14_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Heterodisulfide reductase, subunit A (HdrA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hdrA</name>
        <name evidence="3" type="ordered locus">SSO1131</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A90266">
        <property type="entry name" value="A90266"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009989759.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97Z14"/>
    <dbReference type="SMR" id="Q97Z14"/>
    <dbReference type="FunCoup" id="Q97Z14">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO1131"/>
    <dbReference type="PaxDb" id="273057-SSO1131"/>
    <dbReference type="EnsemblBacteria" id="AAK41384">
        <property type="protein sequence ID" value="AAK41384"/>
        <property type="gene ID" value="SSO1131"/>
    </dbReference>
    <dbReference type="GeneID" id="1454163"/>
    <dbReference type="KEGG" id="sso:SSO1131"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.1124"/>
    <dbReference type="eggNOG" id="arCOG02235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020302_1_0_2"/>
    <dbReference type="InParanoid" id="Q97Z14"/>
    <dbReference type="PhylomeDB" id="Q97Z14"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051691">
        <property type="entry name" value="Metab_Enz_Cyan_OpOx_G3PDH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42949">
        <property type="entry name" value="ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42949:SF3">
        <property type="entry name" value="ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="2">
        <location>
            <begin position="5"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK41384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="368" mass="40665" checksum="519589E19E97E75A" modified="2001-10-01" version="1">MASKSVLVIGAGPAGLSATKELANMGVNVVVVEREPFLGGTPKRLKYSLLFPELRPASEVIDPLVKTVQENGNVKIYTESIVDAAKNTSDGFEISIKDKNGKVKVEKANAIIAASGFEHFDSRRKYEYGYGIIPNIYQISDIEGMLHENKLVTTKGTPPKRVAILLCVGSRDATVGNTYCSRVCCAVSIKQAMEIKQRIPDAVVHIYYMDIRTYGLMEDKLYWKSQLDYRVGYIRGRISEFMRGPNDTVIIKGEDTMNLNRALTVPYDMVILANGMELGLGSKQVAKILGLEFEEHGFVKPLDPDRLPVQSTKKGIFLAGAITGPKTISDSITEGYAAAMKAYEYITHGIWEESDFAKKTAEMKVVHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZI5</accession>
    <name>Q97ZI5_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome assembly chaperone family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0921</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90243">
        <property type="entry name" value="D90243"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009992353.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZI5"/>
    <dbReference type="STRING" id="273057.SSO0921"/>
    <dbReference type="PaxDb" id="273057-SSO0921"/>
    <dbReference type="EnsemblBacteria" id="AAK41203">
        <property type="protein sequence ID" value="AAK41203"/>
        <property type="gene ID" value="SSO0921"/>
    </dbReference>
    <dbReference type="GeneID" id="1455170"/>
    <dbReference type="KEGG" id="sso:SSO0921"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.922"/>
    <dbReference type="eggNOG" id="arCOG00347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075000_0_1_2"/>
    <dbReference type="InParanoid" id="Q97ZI5"/>
    <dbReference type="PhylomeDB" id="Q97ZI5"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610:SF3">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK41203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="28179" checksum="C2C1C37E84076AB3" modified="2001-10-01" version="1">MGRLSEVFEVRERYVPQIGRPSYLLVSLPDAGLVGSISGEFLINYLNLREYGEIYSNKHLPPISHVVNGVAKSPIRLYHYDNFLLLHSWIAIPANSINHLANLVVSYAEKYGIQTIISITGVPVPNRLDLEKPTPYWVASSEDFAKELDSLNLMKKFVEGYVSGPYAPILFQSAKKLIRNLLIVVESFLDLPDPEAAAVALDILSKMLGFKVDTSSLLKEAEEIRERIKGLMQQTRQELPNYSGLRPSTYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZP9</accession>
    <name>Q97ZP9_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable integrase from SSV1 (Orf D-335)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO6468</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK41057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90225">
        <property type="entry name" value="B90225"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009991345.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZP9"/>
    <dbReference type="SMR" id="Q97ZP9"/>
    <dbReference type="PaxDb" id="273057-SSO6468"/>
    <dbReference type="EnsemblBacteria" id="AAK41057">
        <property type="protein sequence ID" value="AAK41057"/>
        <property type="gene ID" value="SSO6468"/>
    </dbReference>
    <dbReference type="GeneID" id="34442650"/>
    <dbReference type="KEGG" id="sso:SSO6468"/>
    <dbReference type="eggNOG" id="arCOG07277">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184189_0_0_2"/>
    <dbReference type="InParanoid" id="Q97ZP9"/>
    <dbReference type="PhylomeDB" id="Q97ZP9"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012922">
        <property type="entry name" value="ORF_D-335"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07935">
        <property type="entry name" value="SSV1_ORF_D-335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ORF D-335-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK41057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="73" mass="8453" checksum="AAC5CC13315CEE83" modified="2001-10-01" version="1">MKETSRYKYGDIIRERKGRYYVYKLETINGEVKETYVGPLVDVVKTYEKLKENGVWVSPTMGPLGFEPRTSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>Q97ZU3</accession>
    <name>Q97ZU3_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec-independent protein translocase protein TatC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">tatC</name>
        <name evidence="6" type="ordered locus">SSO0484</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Forms a complex with TatA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TatC family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F90193">
        <property type="entry name" value="F90193"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010922999.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q97ZU3"/>
    <dbReference type="FunCoup" id="Q97ZU3">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0484"/>
    <dbReference type="PaxDb" id="273057-SSO0484"/>
    <dbReference type="EnsemblBacteria" id="AAK40805">
        <property type="protein sequence ID" value="AAK40805"/>
        <property type="gene ID" value="SSO0484"/>
    </dbReference>
    <dbReference type="GeneID" id="84059029"/>
    <dbReference type="GeneID" id="84061977"/>
    <dbReference type="KEGG" id="sso:SSO0484"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.478"/>
    <dbReference type="eggNOG" id="arCOG01919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031942_3_2_2"/>
    <dbReference type="InParanoid" id="Q97ZU3"/>
    <dbReference type="PhylomeDB" id="Q97ZU3"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009977">
        <property type="term" value="F:proton motive force dependent protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002033">
        <property type="entry name" value="TatC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00945">
        <property type="entry name" value="tatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371:SF0">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01840">
        <property type="entry name" value="TATCFAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAK40805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="294" mass="33405" checksum="163A76543C4698BF" modified="2001-10-01" version="1">MTERTREVEKLEERPLIEHLRELAYRLRRILIALAITFMIYFMLGLELVRVYLPYPFFGLKYVLAEVPVLYPSLFDSISVQLTQLFIYNELPKGVKLIIINLFDPLFASFYISLYLAIFTTIPIIVREIWAFVAPGLYEHEKKLFKSIIFPAFLLFALGSAFAYFLLIPLMLRIILLYATALGSAVEPTLGLRSFVSTVMTLLIATGLSFELPLIMGGLTGVGVVKSITWLKNWRWGVLVSFIIAWIISPGTTGGIIETVIGITLSTLYFIGALISKFMEKGRKKESQKTLERI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2026-01-28" version="132" xmlns="https://uniprot.org/uniprot">
    <accession>Q980F0</accession>
    <name>MOAA_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable GTP 3',8-cyclase</fullName>
            <ecNumber evidence="1">4.1.99.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Molybdenum cofactor biosynthesis protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">moaA</name>
        <name type="ordered locus">SSO0362</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="1"/>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90179">
        <property type="entry name" value="E90179"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990662.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980F0"/>
    <dbReference type="SMR" id="Q980F0"/>
    <dbReference type="FunCoup" id="Q980F0">
        <property type="interactions" value="203"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0362"/>
    <dbReference type="PaxDb" id="273057-SSO0362"/>
    <dbReference type="EnsemblBacteria" id="AAK40692">
        <property type="protein sequence ID" value="AAK40692"/>
        <property type="gene ID" value="SSO0362"/>
    </dbReference>
    <dbReference type="GeneID" id="1455502"/>
    <dbReference type="GeneID" id="44129336"/>
    <dbReference type="KEGG" id="sso:SSO0362"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.356"/>
    <dbReference type="eggNOG" id="arCOG00930">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_0_1_2"/>
    <dbReference type="InParanoid" id="Q980F0"/>
    <dbReference type="PhylomeDB" id="Q980F0"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21117">
        <property type="entry name" value="Twitch_MoaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_A">
        <property type="entry name" value="MoaA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040064">
        <property type="entry name" value="MoaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013485">
        <property type="entry name" value="MoaA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="MoaA_twitch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02668">
        <property type="entry name" value="moaA_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001199">
        <property type="entry name" value="PRK00164.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF0">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01383">
        <property type="entry name" value="cyclic_pyranopterin_phosphate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000153028" description="Probable GTP 3',8-cyclase">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="4"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
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        <location>
            <position position="248"/>
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            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="250"/>
            <end position="252"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="308" mass="35196" checksum="A53FEF977CBAD49B" modified="2001-10-01" version="1">MIDRFGRPLEDLRITLTHACNFECFFCHMEGEEGDNYLLSKEDILLVAKVARKFGINSVKLTGGEPTLRRDLVEIVRGLKQLGYEDVSMTTNGFLLKDLAHKLKLAGLDRINISLHAVSRDTFKKITGVDAFDRVIEGIKSAIDVGLVPVKLNFVVNRRNREEAFKFIELSQNLGVNEIHLIELHPVGLGKLAFKEHDDLREIEEYIEKISIKKLIRKKHFRPRYVLPSGLIVEVVKPYANPIFCAGCNRIRLSVDGKLKTCLYREDNVIDVLDILKGEYSEDVKEELLGRAFMIAIAIREPNFKYKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2026-01-28" version="104" xmlns="https://uniprot.org/uniprot">
    <accession>Q980F8</accession>
    <name>Y352_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-binding protein SSO0352</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">SSO0352</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDCD5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90178">
        <property type="entry name" value="E90178"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990649.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="PDB" id="6IQC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.49 A"/>
        <property type="chains" value="A=1-118"/>
    </dbReference>
    <dbReference type="PDB" id="6IQO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.11 A"/>
        <property type="chains" value="A/B=1-118"/>
    </dbReference>
    <dbReference type="PDBsum" id="6IQC"/>
    <dbReference type="PDBsum" id="6IQO"/>
    <dbReference type="AlphaFoldDB" id="Q980F8"/>
    <dbReference type="SMR" id="Q980F8"/>
    <dbReference type="FunCoup" id="Q980F8">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0352"/>
    <dbReference type="PaxDb" id="273057-SSO0352"/>
    <dbReference type="EnsemblBacteria" id="AAK40684">
        <property type="protein sequence ID" value="AAK40684"/>
        <property type="gene ID" value="SSO0352"/>
    </dbReference>
    <dbReference type="GeneID" id="1455494"/>
    <dbReference type="KEGG" id="sso:SSO0352"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.345"/>
    <dbReference type="eggNOG" id="arCOG04179">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122978_3_0_2"/>
    <dbReference type="InParanoid" id="Q980F8"/>
    <dbReference type="PhylomeDB" id="Q980F8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.140:FF:000004">
        <property type="entry name" value="DNA-binding protein PAE3044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="entry name" value="PDCD5-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="HAMAP" id="MF_00026">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022889">
        <property type="entry name" value="DNA_bind_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003268">
        <property type="entry name" value="PRK04239.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01984">
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    </dbReference>
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        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="Double-stranded DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000121565" description="DNA-binding protein SSO0352">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="9"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="65"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="86"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="90"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00026"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="6IQC"/>
        </source>
    </evidence>
    <sequence length="118" mass="14054" checksum="ADE29463BC47A3EC" modified="2001-10-01" version="1">MSTPNSYDDEELEELLRRKAAQEQKRIEEERKRKAELESQKESILRVILTPEARQRLTNIKLVKPEFAESLENQLIALAQSGRIKIPITDEELKQILEQISQQNRRDFKIQIRERGWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2024-03-27" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q980H8</accession>
    <name>Q980H8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Heavy-metal chelation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">SSO0318</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G90174">
        <property type="entry name" value="G90174"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980H8"/>
    <dbReference type="STRING" id="273057.SSO0318"/>
    <dbReference type="PaxDb" id="273057-SSO0318"/>
    <dbReference type="DNASU" id="1455464"/>
    <dbReference type="EnsemblBacteria" id="AAK40654">
        <property type="protein sequence ID" value="AAK40654"/>
        <property type="gene ID" value="SSO0318"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO0318"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.312"/>
    <dbReference type="eggNOG" id="arCOG03216">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1217596_0_0_2"/>
    <dbReference type="InParanoid" id="Q980H8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007161">
        <property type="entry name" value="DUF364"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025251">
        <property type="entry name" value="DUF4213"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04016">
        <property type="entry name" value="DUF364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13938">
        <property type="entry name" value="DUF4213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159713">
        <property type="entry name" value="Dhaf3308-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4213" evidence="2">
        <location>
            <begin position="10"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Putative heavy-metal chelation" evidence="1">
        <location>
            <begin position="100"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAK40654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="26285" checksum="D3E820B19E536511" modified="2001-10-01" version="1">MTIMILREIIDELSYQLKQRRIINVCVSPAYTSVMLDDQSIGISHTITDGEIEGAGEIIGKNAYDVVINNLDSNLQRSLSLAILNALGSMIDFTQGDPINLYSGGKLCVFGFSPQLSYSNFDSVIVYDFMSTENKKVGNTEIKPYSSLSREVCSTALIFASSIVNNTIDRIISQISANHLILTGISSVDAPITLKNHGFEVLGKLFPVEKYRVFRTICEGGSNKLLSKYIMRYFKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="125" xmlns="https://uniprot.org/uniprot">
    <accession>Q980J1</accession>
    <name>Q980J1_SACS2</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Chorismate mutase/prephenate dehydratase, putative (TyrA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tyrA</name>
        <name evidence="4" type="ordered locus">SSO0302</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A99173">
        <property type="entry name" value="A99173"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990595.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980J1"/>
    <dbReference type="FunCoup" id="Q980J1">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0302"/>
    <dbReference type="PaxDb" id="273057-SSO0302"/>
    <dbReference type="EnsemblBacteria" id="AAK40640">
        <property type="protein sequence ID" value="AAK40640"/>
        <property type="gene ID" value="SSO0302"/>
    </dbReference>
    <dbReference type="GeneID" id="1455450"/>
    <dbReference type="KEGG" id="sso:SSO0302"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.296"/>
    <dbReference type="eggNOG" id="arCOG00245">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_812860_0_0_2"/>
    <dbReference type="InParanoid" id="Q980J1"/>
    <dbReference type="PhylomeDB" id="Q980J1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.59.10">
        <property type="entry name" value="Chorismate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008244">
        <property type="entry name" value="Chor_mut/prephenate_DH_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010950">
        <property type="entry name" value="Chorismate_mutase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036979">
        <property type="entry name" value="CM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050812">
        <property type="entry name" value="Preph/Arog_dehydrog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01791">
        <property type="entry name" value="CM_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363:SF0">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001499">
        <property type="entry name" value="Chor_mut_pdh_Tpr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00830">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="Chorismate mutase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51168">
        <property type="entry name" value="CHORISMATE_MUT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="3">
        <location>
            <begin position="93"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAK40640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <sequence length="346" mass="39380" checksum="00E57BBCC86E88FC" modified="2001-10-01" version="1">MTEEITRLREEIDKVDEQLVKLLSYRLELSRKIGKAKSNSNISVTDENREMKVREKWIANAKKYNIPNSLVESILPLIFSYSKLVQINPGEKERVVIYGYGGMAKSIVSILSLAGHEVSITGRDLSKAEMLANQFKCVSMSLLKAIDWGDIIIFAIPPSAILNNSDELFSKALKDKIVMDISSSKFKIFGFLEELSRKLEFRYISTHPLFGPIEYPIGERVVIIPSKTSSNDDVMKVENFWRKSGLVPVITDVETHEKAMAIVQVLTHYYLLGLSNAIDTLSLELGVDYSNFHTTNFRELNKILKRVKDLKNVITEIQNQNPYSYKVRNIGLEELKKIKEELEGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2025-02-05" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>Q980L8</accession>
    <name>Q980L8_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome assembly chaperone family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">SSO5544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E90169">
        <property type="entry name" value="E90169"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980L8"/>
    <dbReference type="STRING" id="273057.SSO5544"/>
    <dbReference type="PaxDb" id="273057-SSO5544"/>
    <dbReference type="EnsemblBacteria" id="AAK40612">
        <property type="protein sequence ID" value="AAK40612"/>
        <property type="gene ID" value="SSO5544"/>
    </dbReference>
    <dbReference type="KEGG" id="sso:SSO5544"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.268"/>
    <dbReference type="eggNOG" id="arCOG00347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2461931_0_0_2"/>
    <dbReference type="InParanoid" id="Q980L8"/>
    <dbReference type="PhylomeDB" id="Q980L8"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610:SF3">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="46"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAK40612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="9973" checksum="B4EE0B7351CC51AD" modified="2001-10-01" version="1">MVGPLALFTLHSEIEDLPALVLLPYADRERTDPVAAATAIEVLNKMLSLNVSVDELYEEAKRIEEDLQRQMELLQKELSRGSADRVYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-10-01" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q980Q1</accession>
    <name>Q980Q1_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MBL fold metallo-hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">SSO0234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B90165">
        <property type="entry name" value="B90165"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009990491.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q980Q1"/>
    <dbReference type="STRING" id="273057.SSO0234"/>
    <dbReference type="PaxDb" id="273057-SSO0234"/>
    <dbReference type="EnsemblBacteria" id="AAK40577">
        <property type="protein sequence ID" value="AAK40577"/>
        <property type="gene ID" value="SSO0234"/>
    </dbReference>
    <dbReference type="GeneID" id="1455387"/>
    <dbReference type="KEGG" id="sso:SSO0234"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.232"/>
    <dbReference type="eggNOG" id="arCOG05932">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131312_0_0_2"/>
    <dbReference type="InParanoid" id="Q980Q1"/>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAK40577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="20688" checksum="B97567F327E6C1A4" modified="2001-10-01" version="1">MSIAKIQRNVYVIGGKLIDDNDTNAYLVYDDEKNYYTLIDTTTGANIRYVIESLLEILKGKDKLKYVILTSCKFENSGGLYYIYNLFKPITIAHFPDSIMIRKGECENRLYTPSPISLEIKQKQYILDNLLILLSKNITNGNIVVKYNDVLFSGSNTKITQYSKYIKYICDVNKCRSVSD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-04-23" modified="2026-01-28" version="128" xmlns="https://uniprot.org/uniprot">
    <accession>Q9UX32</accession>
    <name>ARLY_SACS2</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate lyase</fullName>
            <shortName evidence="1">ASAL</shortName>
            <ecNumber evidence="1">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argH</name>
        <name type="ordered locus">SSO0639</name>
    </gene>
    <organism>
        <name type="scientific">Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)</name>
        <name type="synonym">Sulfolobus solfataricus</name>
        <dbReference type="NCBI Taxonomy" id="273057"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Saccharolobus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="Genome" volume="43" first="116" last="136">
            <title>Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="Charlebois R.L."/>
                <person name="Singh R.K."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Allard G."/>
                <person name="Chow C."/>
                <person name="Confalonieri F."/>
                <person name="Curtis B."/>
                <person name="Duguet M."/>
                <person name="Erauso G."/>
                <person name="Faguy D."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Gordon P."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C."/>
                <person name="Kushwaha N."/>
                <person name="Lafleur E."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Penny S.L."/>
                <person name="She Q."/>
                <person name="St Jean A."/>
                <person name="van der Oost J."/>
                <person name="Young F."/>
                <person name="Zivanovic Y."/>
                <person name="Doolittle W.F."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
            </authorList>
            <dbReference type="PubMed" id="10701121"/>
            <dbReference type="DOI" id="10.1139/gen-43-1-116"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="7835" last="7840">
            <title>The complete genome of the crenarchaeon Sulfolobus solfataricus P2.</title>
            <authorList>
                <person name="She Q."/>
                <person name="Singh R.K."/>
                <person name="Confalonieri F."/>
                <person name="Zivanovic Y."/>
                <person name="Allard G."/>
                <person name="Awayez M.J."/>
                <person name="Chan-Weiher C.C.-Y."/>
                <person name="Clausen I.G."/>
                <person name="Curtis B.A."/>
                <person name="De Moors A."/>
                <person name="Erauso G."/>
                <person name="Fletcher C."/>
                <person name="Gordon P.M.K."/>
                <person name="Heikamp-de Jong I."/>
                <person name="Jeffries A.C."/>
                <person name="Kozera C.J."/>
                <person name="Medina N."/>
                <person name="Peng X."/>
                <person name="Thi-Ngoc H.P."/>
                <person name="Redder P."/>
                <person name="Schenk M.E."/>
                <person name="Theriault C."/>
                <person name="Tolstrup N."/>
                <person name="Charlebois R.L."/>
                <person name="Doolittle W.F."/>
                <person name="Duguet M."/>
                <person name="Gaasterland T."/>
                <person name="Garrett R.A."/>
                <person name="Ragan M.A."/>
                <person name="Sensen C.W."/>
                <person name="Van der Oost J."/>
            </authorList>
            <dbReference type="PubMed" id="11427726"/>
            <dbReference type="DOI" id="10.1073/pnas.141222098"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 35092 / DSM 1617 / JCM 11322 / P2</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAK40947" version="1"/>
        </conflict>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAB57662" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="Y18930">
        <property type="protein sequence ID" value="CAB57662.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE006641">
        <property type="protein sequence ID" value="AAK40947.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D90211">
        <property type="entry name" value="D90211"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_014511588.1">
        <property type="nucleotide sequence ID" value="NC_002754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9UX32"/>
    <dbReference type="SMR" id="Q9UX32"/>
    <dbReference type="FunCoup" id="Q9UX32">
        <property type="interactions" value="298"/>
    </dbReference>
    <dbReference type="STRING" id="273057.SSO0639"/>
    <dbReference type="PaxDb" id="273057-SSO0639"/>
    <dbReference type="EnsemblBacteria" id="AAK40947">
        <property type="protein sequence ID" value="AAK40947"/>
        <property type="gene ID" value="SSO0639"/>
    </dbReference>
    <dbReference type="GeneID" id="1454914"/>
    <dbReference type="GeneID" id="44129639"/>
    <dbReference type="KEGG" id="sso:SSO0639"/>
    <dbReference type="PATRIC" id="fig|273057.12.peg.644"/>
    <dbReference type="eggNOG" id="arCOG01748">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027272_2_0_2"/>
    <dbReference type="InParanoid" id="Q9UX32"/>
    <dbReference type="PhylomeDB" id="Q9UX32"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001974">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814:SF1">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000137870" description="Argininosuccinate lyase">
        <location>
            <begin position="1"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="444" mass="50263" checksum="817B75F74C3C60CE" modified="2003-04-23" version="2">MLYRKWGSEKDEVVNYTSSVDSDREIIEEVKLTMKAHVISLYLTGYLGKETARKILVALNEFKEIGQGYEDIHEALEDFLIKKVGEDAGWIGLGRSRNDHVATALRLRLRNKLIELLTDINSLRKILLDKAKEHITTIFPSYTHLQLAQPTTFAHYLTYIEEELASRWEIIFSTLKQVNKSPLGSGAIVGTNVKIDREKEAELLGFDSIIYNTLSATSSRADILSTISELTVLMVVLSRIAEDLIFFSSNKLIKLPDSHVSTSSLMPQKRNPVTMEILRAKAAESIGMLTSLLSIYKGLPTGYNLDLQEMNKYYWLVINYTKSSIGVLSSLFSQIQVNKINIDESSLATDDAELLSISKKVPYRSTYFEIAKKVREGSYKSTLKIEDSINMKAVIGSPNFDLMANLIKIRETKLKEDEKEIEEYKLKIISKLGELQVIENEIGE</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>